LOCUS       DAOQNJ010000001        16594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_1330_length_16594_cov_7.690429, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000001 DAOQNJ010000000
VERSION     DAOQNJ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16594
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1330_length_16594_cov_7.690429"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLG52_00005"
     CDS             complement(<1..1120)
                     /locus_tag="PLG52_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNB39847.1"
                     /translation="MPRLTLRPSLLATILLLVTLLASCKSPQLNSDITITVTADGTTR
                     DITVPQGQTVSQALESAGITVNSLDKTEPPLYTVIGNGDLVTLIRVEEVFETEEIVVP
                     FERQVVRNETLPEGETRLVQAGVNGLEEITYRKILEDKVETSQSIVKTTVIKEALPEI
                     VMVGAQTSFVPLNIPGTLAYLAGGNAWIMEGSTANRRVIVSTGDLDGRIFTLSPNGEY
                     LVFTRKSSKPADEEINTLWAVRTRNLDPKLISLQAKNVVHFAAWIPGTNSIAYSTVEP
                     RSAAPGWQANNDLYRVSITNSGSPKRILYPSSGGVYGWWGMNFAFAPDGRLAYSRPDG
                     IGLVDQDSGRLKPLLDITPLNTHSDWAWIPPLVWGADGN"
     gene            complement(1140..1709)
                     /locus_tag="PLG52_00010"
     CDS             complement(1140..1709)
                     /locus_tag="PLG52_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39848.1"
                     /translation="MKTKNYIYLFIVSLVSASLACSINVGGPEYPAQPISASPTDAQA
                     LRDMIQQAMVTGAETGVITLSVTESQLTGYMAEKLSQQTNPPFTDPQILLRNGQMQMY
                     GKVTQGWFTANMLITMNVTIDETTGQPKIQITSADFGPIPAPEGVNNAISAIIEEAFT
                     GSFGPVAVGFRLQTITIADGIMTLTGRIK"
     gene            1754..2323
                     /gene="scpB"
                     /locus_tag="PLG52_00015"
     CDS             1754..2323
                     /gene="scpB"
                     /locus_tag="PLG52_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNB39849.1"
                     /translation="MSESEPITNEELSLAAKIEAMLFVSADPVPVQQLSQALDVTASV
                     VERGLKELDEVLKARGLRLQRNAGRVQLTTAPEIAGLVETFLGLEAITHLSRAALETL
                     AIIAYQQPVTRPQIDSIRGVNSDGMMKSLLSKGLIQESGRTEGPGRPILYSTTPEFLQ
                     HFGLSSIMELPPLATPIETEPEPQELLKG"
     gene            2326..3093
                     /locus_tag="PLG52_00020"
     CDS             2326..3093
                     /locus_tag="PLG52_00020"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB39850.1"
                     /translation="MQERLQKILAQAGYGSRRACEEFIIEGRVSVNGQIAILGQKADL
                     AVDTIKVDGKSLSKPETMTYIALYKPRNVLSAAEGQDDRKTVRDLIPAPGHLYPVGRL
                     DFDSEGLILMTNDGDLTNKLTHPRYAHEKEYRVLLARKPDTEQLDAWRRGVVLPEGDR
                     TQPANVHFISTSGKGAWIRVVMGEGKKRQIREVGKLLGLPVVRIIRVRIGTLKLGDLK
                     PRQWRYLTEDEIRELKGDKVPKMEQKPRPKRTTTRRA"
     gene            3116..4444
                     /locus_tag="PLG52_00025"
     CDS             3116..4444
                     /locus_tag="PLG52_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNB39851.1"
                     /translation="MKRLNFIVIIALVASTLGACQVLPRINDTRPNFLIIVTDDQRYD
                     TMEYMPNTQELIFDQGVTFSKGYITTPFCCPSRASILTGLYAHNHWVQVNEDPLEFRT
                     MVEDIHRNGYYTGLVGKYLNSWKGDMRPEYDFWVSFWGGTMKNYYNPDLNVNGTWSKH
                     TGYSTYLFRDFGVQFIEEASKQSKPFLLMYTPNAPHAPFTPAEEDVSLLTDLAPFRPL
                     SFNEEDVSDKPDSISNMAPLSEEDIKSIDNTRRRQLLTLMALDRTIPDLINKLKETGE
                     LDNTVIIFISDNGKHWGEHRLDSKSTAYEESVKVPFAIRYPPLIPTPYVDNRIVANID
                     IAPTIYELSETRLPDLMDGTSLVSLFSGETEWRDHILLEAWPDRGAWSAIHTGDYVYI
                     ETQGDLSEFYDLTLDPFEMDNAINDPQYAEMIAQLQQYLQTEKQPKTSPP"
     regulatory      4505..4608
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4848..5225
                     /gene="gcvH"
                     /locus_tag="PLG52_00030"
     CDS             4848..5225
                     /gene="gcvH"
                     /locus_tag="PLG52_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNB39852.1"
                     /translation="MNNPTNLKYAKSDEWFDPSTGAVGISDYAQGQLSDIVFVEILVD
                     EGEEVTVGKAIASVESVKASSEIYAPVAGKVTAVNKDLSNTPETINTDPYGAAWMIKI
                     DGSASADLMDAAAYETHCAGRSH"
     gene            5289..6638
                     /gene="gcvPA"
                     /locus_tag="PLG52_00035"
     CDS             5289..6638
                     /gene="gcvPA"
                     /locus_tag="PLG52_00035"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNB39853.1"
                     /translation="MTYIPISPKERDAMLETVGVKSLDDLFDAIPAKHRFPELSLPPA
                     LTEMEAAAHLGDMAATNENVREHLISFLGAGAYHHYVPSVVDHMLRRGEFYTAYTPYQ
                     PEISQGTLQAIFEYQSLMTNLTGMDVSNASHYDGATATAEAVNLAYAQYRGKRKKVVM
                     SPTVHPQYREVIRTYSQGMGIEEAGDDPNVDLEAGPEGLMSLVDDKTMLVVVQYPDFF
                     GRIYDYTKLIEDAHAKGALVCVVANPTALLMLKTPGSMGADIVVGEAQPFGIPLWYGG
                     PYLGFFTTKKSYVHKMAGRLIGETVDVRGNRSYVLTLTAREQHIKRERATSNICSNQG
                     LLALAASVYMSVLGKQGLQEVANLCYQKAHYAANELGKIDGFGLCFNDPFFHEFVLCC
                     PKPASEVNQYLLDNGILGGYDLGKDYPSLKNHLLVAVTEMNSKDDIDTLVEILKEMN"
     gene            6638..8125
                     /gene="gcvPB"
                     /locus_tag="PLG52_00040"
     CDS             6638..8125
                     /gene="gcvPB"
                     /locus_tag="PLG52_00040"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNB39854.1"
                     /translation="MATIIEPTIFELSTPGRRGVKMPASDVPEVALPPKDLLRSELPL
                     PELAEVDVVRHYMKLSTFNYSVDSGFYPLGSCTMKYNPKINEDTCRLPGFLYTHPLQP
                     VETVQGNLALMFETQEWLKEISGFAGVTLQPAAGAHGEFTGVLMMAAYHKLRGDTKRT
                     KMLIPDAAHGTNPASVGMLGMQVVTIPSDSRGNVDLEKLKAACDDTVVGMMLTNPNTL
                     GMFDEHIEEVVNMVKDCGGLMYGDGANLNALLGIVRPGDLGFDVMHFNLHKTFSVPHG
                     GGGPGSGPVGVSQRLADFLPSPLVGIIEEGDEDTAPLYGFVTPKNSIGRMKAFHGHFG
                     GGLVRAYTYIAMHGPDGLKDVSQYAVLNANYLQARLRSTYHIPYDRICMHEFVMEGQF
                     EGSDVRALDISKRLMDYKFHPPTNYFPLIVHEALMIEPTETENKDTLDRFADALIAIA
                     EEAKTQPDLLHNAPSTTQFGRMDEVKAARELVLCCWLPENYEQMV"
     gene            8233..8769
                     /locus_tag="PLG52_00045"
     CDS             8233..8769
                     /locus_tag="PLG52_00045"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB39855.1"
                     /translation="MTTIQTKTEIISALQGSNKHMQEWFSAISAIEYFTRQGDTWSAA
                     DNVDHVIKAVKPIVLALKLPRLVLKIFFGRPQHSSRPYEEVCRIYSEAILKGAKASGQ
                     FLPSENPVQDKERAKTMQLQKAEKIIQDLISALEKWEDSTLDQCQLPHPIIGNLTMRE
                     MLFFTIHHNVRHASHEGD"
     gene            8879..9682
                     /locus_tag="PLG52_00050"
     CDS             8879..9682
                     /locus_tag="PLG52_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39856.1"
                     /translation="MKIFKYWASEKVRTDVYGEIKEFNIYGGSNISTADAAQNAIQKA
                     ELIKRKIEGDRSAFEEYEVDIREEILQVVDDKNIITRNRYGAQVLNTEYMLILDIDKP
                     KPAGLMGLFKKASGESDKDKIFNMVRTQAASPKYAGLGFRLYETYQGARVIVTGRDFN
                     ARESSTFELMKDFNTDPLYAAICRKQGCFRARLTPKPNRVQMKAYKVKYPRSEPDALF
                     DLWLRDYEQASRSFSVCRFVEQIGPSFGTSEALRLHDEITGANLHLPLA"
     gene            9802..10608
                     /locus_tag="PLG52_00055"
     CDS             9802..10608
                     /locus_tag="PLG52_00055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB39857.1"
                     /translation="MLNKILFHRFPNIKSLLTRLQYEFLSTLKLNKDVMFMNFGYTAH
                     HQGQEPIPLDPDDEKHRYPLQLYHHVARHADWTNADALEVSSGRGGGASFIMRHFKPR
                     SYVGVDFSAKAIEFCREHHKVDGLRFEHGNAEDLQFPDNSFDIVVNVEASLYYPNIKK
                     FFQHVKRVLKPGGYFLYTDLRYEEKVEEWHAQIKEMGFKVIKEEDITENVLKAMEMDR
                     ERRIHLVKTYIPRILQKQFFHFAGLDVESPTSAQPHLEQRRYWYFALQKM"
     gene            10680..12368
                     /locus_tag="PLG52_00060"
     CDS             10680..12368
                     /locus_tag="PLG52_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39858.1"
                     /translation="MKKYPFFGLISILVLSSLILSACGVSEDYEEDFSTEEVATEEIV
                     TEEATQASSDATSITPSAPGDVIYDFGFTPEENGFSFENYGDEIPATNLTADELRRMF
                     GDEVCADINGDECILTPPAAQWMEELNTDMSGGHCEGMAVLSLVMYTGQVSPSEFGGN
                     TASELDITDKALQREIAYWWATQAVDPTASSIISGTPMEILDLVEKMDVKGETYTIGI
                     YNENGDGHAITPFGVEDKGDGLYAILVYDNNYPGETRELFIDSRDNSWTYETAINPAV
                     ETDVWSGNADTQTLDLTPTSARLEPQVCPFCEGGYSSVGKLAAATQPLNQIFLDGEGH
                     ILITDDNGNRLGYVDGKIVNEIPGASYTKYRMLANGETPEPIYAVPADLDITVTIDGS
                     ALTEETLTDLVLIGPGYSIGVEGIYLTPGQVDVAYFYPTDEMIAYETTSDESPSIIFG
                     VENPDADYYFEVYGADMIGGGTITASLDSKAGDLLINTEQLTGEGSFNFYLTRITDEA
                     EEEFSAEDITLQEGALIYVNYAEWTDANPEGIYFGVDLNGDGEIDDEYVVDDAN"
     gene            complement(12437..13060)
                     /locus_tag="PLG52_00065"
     CDS             complement(12437..13060)
                     /locus_tag="PLG52_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HNB39859.1"
                     /translation="MSHPVITVTPETPVHDALAMFKKEHIRRAPVVKDGKLVGIVSES
                     DLLNASPSPVTSLSVWEMNYLISKVTVKQVMSKKLITVDVNTPIEEAARIMADKKLGG
                     LPVMRSDKLVGIITETDLFKIFLELMGARTKALRVTAEIDDKPGTLAKLTKAVSDAGG
                     NFISFGQFSGDDTSSKILTFKVAGLKKDEVTSALSKVVKKFWDIRQS"
     gene            complement(13254..14612)
                     /locus_tag="PLG52_00070"
     CDS             complement(13254..14612)
                     /locus_tag="PLG52_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB39860.1"
                     /translation="MSNYDKVDAFIDKNLSQSLDELKKYVAQPSISAQNLGLKECAQL
                     VKEMLEKRGFTAEIMATEGAPVVFAERKGNVDKTILIYNHYDVQPPEPLELWDSPPFE
                     PEIRDGKMYGRGVSDDKGHLTSRLHAIDAILAEDGELPCNIKFIIEGEEETASVHLHE
                     FISNNVERLKADACIWEFGSVDHRDRPQQYLGLRGICYVELSVTSLGTDVHSGIGGSI
                     FPNAAWRLTWALSTLKGVDERIRIPGFYDDVVQPTDRDREFMEKLPDLAEDYKTRFGV
                     QKFIKGLEGGMDLKLEEVFTPTCTICGLTSGYQGPGSKTVQPASASAKVDFRLVPNQM
                     PKDILKKLRAHLDAEGFSDVEIKFLGGEPAARTDPDHPFVKLVSETAEEVYDAKMDIV
                     PMIGGSGPNYPFVHELGLPVVCMGIGYPNTRAHAPNENFRIDLYAQHAKHTARVIREF
                     AK"
     gene            complement(14670..15242)
                     /locus_tag="PLG52_00075"
     CDS             complement(14670..15242)
                     /locus_tag="PLG52_00075"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNB39861.1"
                     /translation="MSPSKKSKPENKVVTAILRILAVLAVIGITVYVYSIRNHVEEFA
                     NYGYFGAFLIMLIANATVILPAPGVAIVFAMGSMLNPLGVALAAGVGGMLGEMTGYLA
                     GYSGQAAVENTTVYNRIVPYVQKYGAWVILVMSAIPNPFFDLAGIAAGIAKIPVWQFM
                     LACWVGQTIKMSMFAFAGAYSIDWIARLYK"
     gene            complement(15220..15642)
                     /locus_tag="PLG52_00080"
     CDS             complement(15220..15642)
                     /locus_tag="PLG52_00080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB39862.1"
                     /translation="MVIELFADKAPVTVNNFVFLSREGYYDGVIFHRVISNFMAQGGD
                     PTGTGRGGPGYKFQDEFHPSLKHSKRGILSMANAGPGTNGSQFFITHGPTPHLDNRHT
                     VFGEVVEGEDVLMSIPERDPMKLNAPAVKIIRVTIEEI"
     gene            complement(15766..16389)
                     /gene="udk"
                     /locus_tag="PLG52_00085"
     CDS             complement(15766..16389)
                     /gene="udk"
                     /locus_tag="PLG52_00085"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNB39863.1"
                     /translation="MMHKIPLIIGVAGGSGSGKTTVAQELLRRVGADRIAYLQHDSYY
                     KDLRMLPSTQHSEINFDHPDTLETELLIKHIIDLREFKAVQIPIYDFSTDTRKTETIT
                     VEPRNVILVEGILLYVEPELRKLFDVKLFVDADADIRFIRRLHRDITERGRSTESVIE
                     QYYATVRPMHLEFVEPSKRYADVIIPEGGHNVAALDMVTARIELLLK"
BASE COUNT         4366 a         3843 c         3995 g         4390 t
ORIGIN      
        1 cgtttccgtc tgcgccccaa acgagaggcg gaatccacgc ccagtcacta tgcgtattca
       61 gcggagtaat atctagtaat ggttttaagc gaccgctatc ttgatccact aagccaattc
      121 catcaggacg agaataagcg agcctaccat ctggggcgaa ggcaaaattc atgccccacc
      181 aaccatacac accaccactg cttggatata agatgcgttt gggtgaacca ctgtttgtaa
      241 tgctgacgcg gtacaggtca ttgtttgctt gccaacctgg tgccgcactg cgcggctcaa
      301 cggtggaata cgcgatcgaa ttcgtgcctg gaatccatgc tgcaaagtga actacatttt
      361 ttgcttgcaa tgagatcaat ttagggtcaa ggttgcgggt gcgaactgcc caaagggtat
      421 tgatctcttc gtctgctggc ttgctggatt tgcgcgtaaa aaccaaatat tcaccgttag
      481 gcgagagagt gaagatgcgc ccatctaaat ctccggtgct gacgataaca cggcgattgg
      541 cggtggagcc ttccatgatc caagcattgc cgccagctag ataggctaac gtgccgggaa
      601 tgtttagcgg cacaaaagaa gtttgtgcgc cgaccatgac aatctcaggt aaagcttctt
      661 tgatcaccgt agtttttaca atggactgac tggtttctac tttatcttct aatatcttgc
      721 gatatgtgat ttcttcaagt ccgttcacgc ccgcctgaac gagacgggtt tcgccttcgg
      781 gcaaggtttc gttgcggacg acttgtcgtt caaacgggac gacgatctct tcggtttcga
      841 aaacttcttc cacccgaatt aaggtgacga ggtcgccatt gccaatgacg gtatagagcg
      901 gcggctctgt cttatcgagg ctgtttacgg tgatccctgc actttccaac gcctgtgaga
      961 cggtttgccc ttgcggaaca gtgatgtctc tagtagtgcc atctgcagtg actgtgattg
     1021 taatgtcact attgagctgc ggcgacttac aagaagccaa cagtgtaacc aaaagcaaga
     1081 tggtggcgag caagctcggg cggagggtga ggcggggcat gtcgggtata atccagccgc
     1141 tatttgatgc gcccggtcag ggtcatgatg ccgtctgcga tggtgatggt ttgtaatcga
     1201 aagccaacag ctacgggacc gaatgaaccg gtgaaggctt cttcaataat ggcactaatg
     1261 gcgttgttga caccttctgg cgcagggatg ggaccaaagt ctgcagatgt gatctgtatt
     1321 ttgggttgac ctgtggtctc atcgatcgtt acgttcatgg taatgagcat attggctgta
     1381 aaccaaccct gtgtaacttt gccgtacatt tgcatttgcc cgttgcgaag caagatttgt
     1441 ggatcagtaa acggcgggtt tgtttgttga gaaagttttt ctgccatata gccggttagc
     1501 tgactttcgg tgacggaaag ggtgattacg ccagtttcgg ctccagtgac catggcttgt
     1561 tggatcatat cgcgtagtgc ttgggcgtcg gttggtgagg ctgaaatggg ctgagccgga
     1621 tattctggtc cgccgacatt gatagagcat gctagtgatg cactgacaag cgaaacaata
     1681 aaaaggtaaa tatagttttt agttttcatg attttgtgaa cgtttgaaat atggactgcg
     1741 gagcaattat agcatgagcg aatctgaacc cattacaaat gaagaattga gcctggcggc
     1801 aaaaatcgag gcgatgttat tcgtttcagc ggacccggta ccagtgcaac aactgtcgca
     1861 ggcgttggat gtgactgcgt ctgttgtgga acgtgggctg aaagaattag acgaagtatt
     1921 aaaagctcgt ggcttgcggc ttcagcgcaa tgcagggcgt gtgcagttga ccaccgcccc
     1981 tgagattgct ggattggtgg aaaccttcct tgggttggag gctattacgc acctcagccg
     2041 agcagcgtta gaaacattgg cgatcattgc ttaccaacag ccggtgacgc gtccacagat
     2101 cgattcgatt cgtggtgtga atagcgacgg tatgatgaaa agcttgttga gcaaaggctt
     2161 gattcaggaa tctggacgca cagaaggtcc gggtcgtcct attctttatt caaccacgcc
     2221 ggaattttta cagcactttg gtttgagctc cattatggaa ttgccgccat tggcaacccc
     2281 aattgaaacc gaacctgaac cgcaagaatt gttgaaaggt taattatgca ggaacgtttg
     2341 caaaagattc tcgcacaggc tgggtatggt tcccgccgtg catgcgagga gtttattatt
     2401 gaggggcgag tttcggtcaa tgggcagatt gcaatcttgg ggcagaaggc agatctggca
     2461 gtggatacga ttaaggtgga cggtaagtcg ctttcaaagc ctgaaacgat gacgtatatc
     2521 gccttataca agcctcgtaa tgtcctctct gctgcagagg ggcaagacga tcgtaaaacg
     2581 gttcgagatt tgattcccgc tcctggacat ttatatccag taggacgcct cgattttgac
     2641 tccgaaggct tgattctcat gaccaatgat ggggatctca cgaacaagtt gacacatccg
     2701 cgatatgcac atgaaaaaga atatcgcgtg ctgctcgctc gtaaaccgga tactgaacag
     2761 cttgatgctt ggcgccgagg cgttgtgttg ccagaaggtg accgtaccca gcctgcgaat
     2821 gttcacttta tttccacatc tggcaaaggt gcatggattc gtgtggtgat gggggaaggg
     2881 aagaaacgtc agattcggga agtgggtaaa ttgcttggtt tgcctgtggt acgcattatt
     2941 cgtgtgcgta tcggcacatt aaaattaggt gatttgaaac cccgacaatg gcgatattta
     3001 actgaggatg agattcggga attaaaaggg gataaggttc cgaagatgga gcaaaagcca
     3061 cgcccgaaac gaacaacaac ccgtcgggcg taacggtctc ttacaaggta ctgtaatgaa
     3121 aagactcaat tttatagtca ttattgcact ggtagcttcc acgttaggag cttgccaggt
     3181 ccttccgcgt atcaatgata cacgcccgaa tttcctcatc attgtgacag atgaccagcg
     3241 ttatgacaca atggaataca tgcctaacac tcaagaatta atatttgacc agggcgtgac
     3301 cttttccaaa ggatacatca ccactccgtt ttgttgtccg agccgtgcca gcattctaac
     3361 cgggttgtat gctcataatc attgggtgca ggtcaatgaa gacccactcg aattccggac
     3421 gatggtggaa gatattcacc gcaacggcta ttacactggc ttggttggga aatacctgaa
     3481 ttcatggaaa ggggatatgc gccccgaata tgatttttgg gtctcatttt ggggtggaac
     3541 catgaagaac tattacaatc ccgatttgaa tgtgaatggg acatggtcca aacataccgg
     3601 gtattcaacc tatttgttta gagactttgg cgttcagttt attgaagaag ctagtaagca
     3661 gtcgaagcct tttcttctga tgtatacgcc aaatgcgccg catgctcctt ttacgcctgc
     3721 ggaagaagat gtcagtttac taactgactt ggctcctttc cgcccattga gtttcaacga
     3781 agaagatgtt tctgataagc cagattcgat ctccaatatg gctcccttat ccgaagagga
     3841 tattaagtct attgataata cgcgtagacg tcagttgctg actctcatgg cgcttgatcg
     3901 caccatccct gacctcatca ataaattgaa agaaacgggc gagttagata acactgtcat
     3961 tatttttatc tcggataatg gcaagcattg gggcgagcat cgcttggact caaaaagcac
     4021 agcgtatgaa gaatcggtta aggtgccatt tgcgatacgt tatccacctc tgattccaac
     4081 tccatatgtg gataatcgca tcgttgctaa tattgatatt gcaccgacga tttatgaact
     4141 atctgaaacg cgtttgccgg acttaatgga cgggacttct ttagtttccc ttttttcggg
     4201 ggaaaccgaa tggcgagacc acatcctgct cgaagcctgg ccagaccgtg gtgcttggtc
     4261 tgccattcat acgggtgatt acgtttatat tgaaacacag ggcgatctgt ctgagtttta
     4321 tgatttaacg cttgatccct tcgaaatgga caatgccatc aatgacccac agtatgcaga
     4381 gatgattgcc caattacagc aataccttca aacagagaag cagccaaaaa cttcgccgcc
     4441 ttaatagtta catttctccc ttgactctgt cctattcaaa ccttagaatt tgaataacaa
     4501 ttgaacggat tgcagggaag agatccccgc aagaggacgc cgaaggtgca aatctggaat
     4561 caggcgaaac cagatgaatc tctcaggcaa aaggaccctg caaccgcaac cactctggaa
     4621 aactccccgc caaagggata ccgacggtgt aaagttagtt cggtagtcag atagtctgat
     4681 agtgaacgcg ttaggtagtc atatacatgg cttcaaaaca aaagagctat tagatgaaat
     4741 aactacaaga ctaccccaat ctctcaggtt ccattacaga ggacgcgcga taataatatt
     4801 gcgcgtcctc ttttgattcg ccaatacctc acactaggag acataaaatg aacaacccca
     4861 ccaacctcaa gtacgcgaaa tctgacgaat ggtttgaccc atccactggc gccgtcggca
     4921 tttctgatta tgctcaggga caactctctg atattgtttt tgttgaaatt ctggttgacg
     4981 aaggggagga agtgactgtt ggaaaagcta ttgcttctgt ggagtctgta aaagcttcgt
     5041 ctgagattta tgctcccgtg gctggcaagg tcactgctgt gaataaagac ctctccaata
     5101 ctcctgaaac cattaacacc gatccctatg gtgcggcgtg gatgatcaag atcgatggta
     5161 gcgcttctgc cgatttaatg gatgccgctg cctacgaaac tcattgtgcc ggaagaagcc
     5221 actaactatt gcattcagcc atcagcttat tgttgaaagc tggcggctga tcgctttgga
     5281 gttttattat gacctatatt ccaattagcc cgaaagaacg ggatgcgatg ttggagactg
     5341 tcggcgttaa gtcgctcgac gatctctttg atgcaattcc agccaaacat cgtttccctg
     5401 agttgagctt gccgcctgcg ctcactgaaa tggaagctgc tgctcattta ggcgatatgg
     5461 ctgccacgaa tgagaatgtg cgcgagcatc taatctcttt cctcggtgcg ggtgcctatc
     5521 atcactacgt tccttctgta gtagatcaca tgctacgccg tggtgagttc tatactgctt
     5581 atactcccta tcagcctgaa atttcacaag gtactttgca agccatcttt gaatatcaat
     5641 cgctgatgac caacctcact ggcatggacg tctctaatgc atctcactac gatggcgcaa
     5701 cagctactgc tgaagccgtg aaccttgctt atgcccagta tcgcggcaaa cgcaagaagg
     5761 tcgttatgtc gccaactgtc cacccacagt atcgcgaagt gattcgtacc tactcacagg
     5821 ggatgggaat tgaagaagcg ggagacgacc caaacgtcga tcttgaggcg ggacccgaag
     5881 gattgatgtc gcttgtagat gacaagacca tgctcgtcgt ggtgcaatat cccgatttct
     5941 ttgggcgtat ctacgattac accaaactca tcgaagatgc tcatgcaaaa ggcgcattgg
     6001 tttgtgttgt tgcgaatccc accgccttgc tcatgctcaa aacgcccggc tcgatgggcg
     6061 cggatattgt tgtgggcgaa gcgcagccgt ttggcattcc gctctggtat ggcggaccct
     6121 accttggctt cttcaccacc aagaaatctt atgttcataa aatggcaggg cgtcttatcg
     6181 gtgaaacggt ggacgtacgc ggcaatcgct cttatgtgtt gactctcact gcacgtgaac
     6241 aacacatcaa acgcgagcgt gccacgtcta acatctgctc caaccaaggt ttactggcgc
     6301 ttgccgcatc ggtatatatg tctgtgcttg gcaagcaagg tttgcaagag gtcgccaacc
     6361 tgtgctacca aaaagcgcac tacgccgcca atgaacttgg caagatcgat ggctttggtc
     6421 tatgcttcaa cgacccgttc ttccatgaat ttgtgttgtg ttgccccaag cctgccagcg
     6481 aagtgaatca atacttgctc gataacggca tccttggcgg ttatgacctc ggcaaagatt
     6541 atccttcgct caagaatcat ttgcttgtgg cagtcactga aatgaacagc aaagatgata
     6601 ttgatactct tgttgaaatc ttaaaggaga tgaactaatg gctacgatta ttgaaccgac
     6661 catttttgaa ctctccactc cagggcgacg cggcgtgaaa atgcccgctt cagacgtgcc
     6721 cgaagttgct ctgcctccca aagatttgct tcgctctgag ttgcctctcc ccgaactcgc
     6781 cgaagtggac gtggttcgtc actacatgaa gctctccact ttcaactatt cggtggatag
     6841 tggcttctat cctttgggtt cctgtactat gaagtacaac ccgaagatca acgaagacac
     6901 ctgccgattg ccgggcttcc tctacactca tccattgcag cctgttgaaa ccgtgcaagg
     6961 taacctcgcc ttgatgttcg agacgcaaga atggctcaaa gagatcagcg gttttgctgg
     7021 cgtgaccctt cagcctgcgg ctggtgcaca cggcgaattc accggcgtct tgatgatggc
     7081 ggcatatcac aaattgcgcg gtgacaccaa acgcaccaag atgcttatcc ctgatgcagc
     7141 ccatggaacc aatcccgctt ccgttggtat gcttggtatg caagttgtga ccatcccctc
     7201 cgactcgcgc ggtaatgtcg acttggaaaa actcaaagcc gcctgtgacg ataccgtggt
     7261 cggcatgatg ctcaccaacc cgaacaccct tggcatgttc gacgagcaca tcgaagaagt
     7321 tgtaaacatg gtcaaggatt gcggtggttt gatgtacggt gacggcgcga acctcaatgc
     7381 gctgctcggc atcgtccgcc caggtgacct tggcttcgac gtgatgcact tcaatttaca
     7441 caaaaccttc tccgttccgc atggcggcgg tggacctggt tctggtccag tcggtgtcag
     7501 ccagcgcttg gcagatttcc tcccatctcc attggttgga atcattgagg aaggggacga
     7561 agataccgcc ccgttgtatg gttttgtgac ccctaagaac agcattggac gtatgaaagc
     7621 gttccacgga cactttggcg gcggtcttgt ccgcgcatac acatacattg ccatgcacgg
     7681 acccgatggc ttgaaagatg tctcgcagta tgctgtgctc aatgctaatt acttgcaggc
     7741 acgtttacgt agtacttatc acatcccata tgaccgtatc tgcatgcatg agttcgtaat
     7801 ggaaggacag ttcgaaggtt cagatgtgcg cgcattggat atttccaagc gcttgatgga
     7861 ttataaattc catccgccta caaactactt cccccttatt gttcacgagg cactgatgat
     7921 cgaaccaacc gaaaccgaga acaaagatac cctcgaccgt tttgccgacg cgcttatcgc
     7981 cattgcggaa gaagccaaga cccagcccga cctgctccac aacgcaccca gcaccacgca
     8041 attcggtcgc atggacgagg tcaaagccgc gagagagttg gtcctctgct gctggctgcc
     8101 tgagaattac gaacagatgg tgtagcgaat taccgtagat taattattgg cagggctggt
     8161 atttgccagc cctgcctgta tttaagaaac ttcttgttga tttaatggtt taaaccgtaa
     8221 aggaaaaact ttatgacaac catccaaacc aaaactgaga ttatttccgc tttgcaaggc
     8281 tcaaataagc atatgcaaga atggttttct gctatttctg ctatagagta tttcacgcgc
     8341 caaggagata cttggtctgc cgctgataat gttgaccatg tcattaaagc cgtgaagccg
     8401 attgtgctag ctttaaagtt accgcgcttg gtattgaaaa tcttctttgg aagaccacaa
     8461 cattcttcga gaccctatga agaagtatgc agaatttatt cagaagcaat tttaaaagga
     8521 gcaaaagcct cagggcaatt cctgccaagc gaaaaccctg ttcaagataa agaacgggct
     8581 aaaaccatgc aattgcagaa ggcagaaaaa attattcaag acttaatttc tgctttagaa
     8641 aaatgggaag atagcactct tgatcagtgt cagttacccc atccgattat tggaaacctg
     8701 accatgcgag agatgctttt ctttaccatt catcataatg tacgtcatgc aagccatgaa
     8761 ggagactagt atctggaaca tcctccgtat ccccaatatg gaggatgttt ttatttaaga
     8821 gctgcccata taattttttt gagttttatg tagatttgat gaacaaagga gaaaatatat
     8881 gaaaatattc aaatattggg cttcagaaaa agtacgaaca gatgtttacg gggagatcaa
     8941 agaattcaat atctatggcg gctcaaatat ctcaacagct gatgctgcgc aaaatgcaat
     9001 tcaaaaagcg gagttaatca aaagaaaaat tgaaggggat agaagcgctt ttgaagaata
     9061 tgaagtggat attcgcgaag agatacttca agtggtggat gataagaaca ttattacccg
     9121 taatcgatat ggcgctcaag tcttgaacac ggaatacatg cttattcttg atatcgacaa
     9181 accaaaacca gccggtttaa tggggctttt taagaaggcg tcgggtgaaa gtgataaaga
     9241 caaaattttc aacatggtgc gaactcaggc ggctagcccc aaatatgccg ggctaggctt
     9301 tcgcctttac gagacctatc aaggtgcacg tgtaatcgta accggtagag actttaatgc
     9361 ccgtgagagt tctacatttg aactgatgaa agactttaat acagaccctt tgtatgcagc
     9421 gatctgccgt aaacagggat gttttcgtgc cagacttact ccaaaaccaa atcgagttca
     9481 aatgaaggct tataaggtga aatatccgcg tagtgagcca gatgcgcttt tcgacctttg
     9541 gctgcgtgat tacgaacagg cgagtcgatc tttcagtgta tgcaggtttg tggagcaaat
     9601 cggaccaagt ttcggtacgt ctgaagcgct ccgtttacat gatgaaataa caggcgctaa
     9661 tcttcacctt ccgttagcgt agttacctcg tcagctttga aaggtaatgt tttcaagaaa
     9721 tgctcgaggt tataattgat ataaccatac aaaaagcatc caattttgga tgctttttgt
     9781 caactttata taggagagag aatgcttaat aaaatattgt tccatcgctt cccgaatatc
     9841 aaaagtctgt tgacccgctt gcaatacgag tttctctcca cgttgaagtt aaacaaagac
     9901 gtgatgttta tgaattttgg gtataccgct caccatcagg gacaagagcc cattccgctt
     9961 gaccctgatg atgagaaaca tcgctatccg ctgcagttgt atcatcacgt cgcccggcat
    10021 gcagattgga ccaatgctga cgcgttagag gtcagctcgg ggcggggagg aggcgcgtct
    10081 tttatcatgc gtcatttcaa accacgttct tacgtcggcg tggatttttc tgccaaggct
    10141 atcgagttct gccgtgaaca tcataaagtg gatggacttc gatttgagca tggcaacgca
    10201 gaagatttgc aatttcccga taactctttt gatattgtgg tcaatgtaga ggcatctttg
    10261 tattacccga atatcaagaa gtttttccag catgtgaaac gtgtcttgaa gccgggtgga
    10321 tatttcttgt acaccgacct gcgctacgaa gaaaaagtgg aagaatggca tgcacaaatt
    10381 aaagagatgg gcttcaaagt gatcaaggaa gaagatatta cagagaatgt tctcaaagca
    10441 atggaaatgg atcgcgagcg ccgtattcat cttgtgaaga cctatattcc gcgcattttg
    10501 caaaaacagt tcttccattt tgccgggttg gatgtagagt ctccaacttc agcacagcct
    10561 catttagagc aacgccgtta ttggtatttt gctttacaaa agatgtaaaa ataataagaa
    10621 aaatagcctg cgtgacgatt gttacgcagg tttgatgtaa taaattcact agaggaaata
    10681 tgaaaaagta cccgtttttt ggattgatct cgattttggt tttgagtagt ttgattcttt
    10741 cagcttgtgg cgtttcagaa gactatgaag aagacttctc cactgaagaa gttgctacag
    10801 aagaaattgt cacagaagaa gcgacacaag caagtagtga tgcgacttct ataacaccca
    10861 gcgcccctgg cgatgtgatt tacgattttg gctttacccc agaagaaaac gggtttagtt
    10921 ttgaaaacta tggtgatgag atccctgcta ctaatttgac ggcagatgag ttacgccgta
    10981 tgtttggtga tgaagtttgt gcagatatta atggggatga atgtattctc accccgccag
    11041 cagcacagtg gatggaggag ctcaacaccg atatgtccgg tggtcattgc gaaggcatgg
    11101 ctgtgttaag cttggtgatg tataccggtc aggtttctcc ctctgagttt ggtggaaata
    11161 cggctagtga attagatatc actgataaag cactgcaacg tgaaattgcg tactggtggg
    11221 caacccaagc cgtagaccca acagcgtcca gcatcatcag cggcacaccc atggagattt
    11281 tggatctagt tgaaaaaatg gatgtgaagg gcgaaaccta taccattggt atttataatg
    11341 aaaacggcga tggtcatgcc attactccgt ttggggtcga agacaaaggg gatggtttgt
    11401 atgctattct ggtttacgac aataactacc ctggcgagac ccgcgagtta ttcatcgact
    11461 cacgtgataa ttcttggacc tacgaaactg caatcaaccc agcagtggag acggacgtct
    11521 ggtctggaaa tgcagatact caaaccctcg atctgactcc gacttcagct cgcctcgaac
    11581 cccaagtttg ccccttctgt gaaggcggat actctagtgt aggcaaactt gccgcagcaa
    11641 cccagcctct taaccagatc ttcttggatg gcgaaggtca catactgatt accgatgaca
    11701 atggcaatcg ccttggctat gtagacggca agattgtgaa tgaaattccc ggcgcctcct
    11761 ataccaaata tcgtatgctt gccaacggag agacccccga gcctatttat gccgtccctg
    11821 ctgatttaga tatcaccgtt accattgacg gctccgcctt gaccgaagaa accctcaccg
    11881 acctcgtttt gatcggaccc ggctattcta ttggagtgga aggcatttat ctcacaccgg
    11941 gtcaggtgga tgtggcgtat ttctacccaa cggatgaaat gattgcttat gaaaccactt
    12001 ccgatgaatc tcccagcatt attttcggtg tggaaaatcc agatgcggat tactacttcg
    12061 aagtgtatgg cgccgatatg atcggcggcg gaaccattac cgcctcactt gatagcaagg
    12121 ctggagactt gctcatcaat acggaacaat tgactggcga aggctctttc aacttctatc
    12181 tgacccgcat tacggatgaa gccgaagaag aattcagcgc cgaagacatt actttgcaag
    12241 aaggcgcatt gatctacgtc aactacgccg aatggaccga tgccaaccct gaaggtatct
    12301 actttggcgt ggacctcaat ggagacggcg agatcgatga cgaatatgta gtagatgatg
    12361 ccaactaagc tattgaacac ttaaataaaa agagcgcatg gagaggagaa gtccatgcgc
    12421 tcttttcttt gttaatttag ctctggcgaa tatcccagaa tttttttaca accttgctca
    12481 atgcactggt aacttcatct ttcttcaaac cagctacctt gaaagtgagg atcttgctgg
    12541 aagtatcgtc cccagagaat tgaccaaatg agatgaaatt gccacctgca tctgataccg
    12601 ccttggtgag tttggcaaga gtaccgggtt tgtcgtcaat ttcggcagtc acacgcaaag
    12661 ctttggtgcg ggcgcccatc agttcaagaa aaatcttgaa gagatcggtc tcagtaatga
    12721 tgcctacgag tttatcggag cgcataacag gcaaaccacc taatttttta tctgccataa
    12781 tgcgggcagc ttcttcgata ggggtgttga catcgacggt gatcaacttc ttgctcataa
    12841 cctgtttgac ggtgacttta ctaataaggt agttcatttc ccacacgctc aagctggtga
    12901 cgggagaagg tgaggcgttc aaaaggtcac tttcagaaac aatgccgaca agttttccgt
    12961 ctttgacaac aggtgcccgg cggatgtgtt ctttcttgaa catagcaagt gcatcatgaa
    13021 ccggggtttc cggagtgacg gtaattacag ggtgagacat tctttcgccg acgaacattg
    13081 tttcctccaa tggggtgaag ttatattgtg aaatttatta caatcacatt atacgcagaa
    13141 tttgcaaaaa gaaagtcact aaattggagt gaaaaaggtc actttaggca tgtcattggc
    13201 gaccgataca aaagaccacg aacaatgatt gctcgtggtc ttttgtcttg tacttacttg
    13261 gcaaactctc gaataaccct tgccgtatgc ttggcatgtt gcgcataaag atcaatacgg
    13321 aagttctcgt taggggcgtg cgcccttgta ttcgggtacc caattcccat gcacacaacg
    13381 ggcaatccaa gttcatgcac gaacggataa ttaggtccag aaccgccgat catggggaca
    13441 atgtccatct tcgcatcgta gacttcttct gcagtttcac tgaccagttt gacgaaagga
    13501 tgatccggat ctgtccttgc cgcgggctcg ccacccaaga atttaatttc gacgtcgctg
    13561 aagccttcgg catccaaatg ggcgcgcagt tttttgagga tgtctttcgg catttgattc
    13621 ggcacaaggc ggaagtccac ttttgcagag gcagaggcag gctgaacggt tttcgagccg
    13681 ggaccttggt atccgctggt gagaccgcaa atggtgcaag taggcgtgaa aacttcttca
    13741 agtttcaaat ccatgccgcc ttccaagcct ttgatgaatt tctgcacccc aaagcgggtt
    13801 ttgtaatctt ctgccaggtc agggagtttt tccatgaact cacggtcgcg gtctgttggt
    13861 tgcaccacat catcataaaa accgggaatg cgaatccttt catccacgcc tttgagcgtg
    13921 ctcagtgccc aggtcaatct ccatgcggca ttggggaaga tgctgccacc aatgccagag
    13981 tgaacgtctg tgcccagtga ggtgaccgat aattccacat agcaaattcc acgcagcccc
    14041 aaatactgct gtgggcggtc gcgatgatca acactgccaa attcccaaat gcaggcatcg
    14101 gctttgagtc gttctacatt attggagata aactcgtgca agtgcacact ggcggtttct
    14161 tcttcgcctt caatgatgaa cttgatattg caaggtaatt cgccatcttc tgcaaggatc
    14221 gcatcgatcg catgcaagcg tgatgtgaga tgacctttat catcgctcac accgcgccca
    14281 tacatcttgc catcccgaat ctctggctca aaaggcgggc tatcccataa ttccaatggc
    14341 tctggcggct gaacgtcgta atgattgtag ataaggatgg ttttatccac gttgcctttg
    14401 cgttctgcaa agacaacagg cgctccctct gttgccatga tctcagctgt gaacccccgc
    14461 ttttccagca tttcttttac aagttgggca cattccttca gtcccaagtt ttgagcgctg
    14521 atacttggtt gggcaacata ctttttgagt tcatctaaac tctggcttag atttttatcg
    14581 atgaaggcat cgactttgtc ataattgctc atctgaaact cctttgctga gataaaaata
    14641 aacctgacag agcacctgtc aggttttggt tatttatata aacgggcgat ccagtcaatg
    14701 gaataggctc cggcaaaagc aaacatagac attttgattg tttgcccaac ccagcatgcc
    14761 aacataaact gccagacggg aattttcgca atgcctgctg caatgccagc caagtcaaaa
    14821 aatggatttg gaattgccga cataacgaga atgacccagg caccatactt ttgcacatat
    14881 ggcacaatgc ggttgtagac cgttgtattt tctacagcgg cttgcccgct atagcctgcc
    14941 aaatagcctg tcatttcacc taacataccg ccgacccctg cagccagtgc aaccccaagt
    15001 ggattcagca tactgcccat ggcgaaaaca atcgccacac ccggagccgg tagaatcacg
    15061 gttgcatttg caatcaacat gatgaggaac gcgccaaaat aaccataatt ggcaaattcc
    15121 tctacgtgat tacggatact gtacacatag accgtaatgc caatcaccgc cagtactgcc
    15181 agaatgcgca gtatggcggt gaccacttta ttttcaggct tagatttctt cgatggtgac
    15241 acggatgatc tttacagccg gggcgttgag cttcatcggg tcgcgttcag gaatggacat
    15301 caacacatct tcgccttcca ccacttcacc gaacacggtg tgacggttat ccaagtgcgg
    15361 ggtgggtccg tgagtaatga agaactgcga accgtttgtg ccgggtcctg cattcgccat
    15421 ggaaaggatg ccgcgcttgc tatgcttcag gcttgggtgg aattcatcct gaaatttata
    15481 gccgggtccg ccgcgtccgg taccggtcgg gtcgccgcct tgtgccataa agtttgagat
    15541 cacacgatga aagattacac cgtcataata gccttcgcgc gaaagaaaca caaagttatt
    15601 cacggttaca ggcgccttat cagcgaacag ttcgatcacc atcgtaccgt tatctgtttc
    15661 catgtgagct ttgtattttt tcttggggtc gatctgcatt gccggggggg tgctccattg
    15721 ttttgccata cgtctctcct tgagtagatg aatataaaat tgggtttatt tgagcagcaa
    15781 ttcgattctt gctgtaacca tatccagagc ggcaacgtta tgcccgccct ctgggataat
    15841 gacatctgca tagcgctttg aaggttctac aaattccaaa tgcattgggc gcacagttgc
    15901 ataatattgt tcgataacag attcagtgga tcgtccgcgt tcggtgatgt ctctgtgtag
    15961 gcggcgaatg aagcgaatgt ctgcatccgc atctacaaaa agttttacat caaaaagctt
    16021 ccgtagctca ggctccacat acaaaagaat cccttccact aaaatcacat ttcggggttc
    16081 tacagtgatt gtctctgtct tacgtgtgtc cgttgaaaaa tcataaatcg gaatctgcac
    16141 ggctttgaac tcgcgcaagt caataatatg tttgattaat aactcagtct caagcgtgtc
    16201 tggatgatca aaattgattt cagaatgttg cgtagatggc aacattcgca aatctttgta
    16261 atatgaatcg tgttgaagat aagcaattcg atctgccccc actcttctta ataattcctg
    16321 tgcaaccgtt gttttgcccg aacctgaccc gcccgcaact ccaataatca aagggatttt
    16381 atgcatcata tgacgagatt atacccgcca ctctcattgg gatgcaaacg gctccggacg
    16441 aagccggagc ctgtttccta accattgaac tttattttcg ctggaagggt ttcgtcagtg
    16501 cattccaaat tgggcaacgg tcgtaaatgc ccaaaaaagc aacaaccgcg ccaatcgcta
    16561 caacgatcca atttctgtca ggcaaaccat acat
//
LOCUS       DAOQNJ010000002        15433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_1520_length_15433_cov_9.869879, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000002 DAOQNJ010000000
VERSION     DAOQNJ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1520_length_15433_cov_9.869879"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..3282)
                     /locus_tag="PLG52_00090"
     CDS             complement(55..3282)
                     /locus_tag="PLG52_00090"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNB39864.1"
                     /translation="MLGVTLSLLLSLLVPSGWVSARPEQALGTISFVPNIETAGVVVS
                     GTGLPATAQLSYRRAGDVNWQPGHALLRIKDGRLIGSLFNLAASTSYEVRVSDGVTEV
                     SGSFTTQADELLFAPTNIIYVNASAQASGDGSASAPFRTIQEGVNRAAPGTQVLVADG
                     IYSESVTFPASGTAGSWIQVKAQGGGAILDGSASVLATDWNPVEGKANVWFTKISPGI
                     KYLARDGLRFYQYDFLSDLYDQRGHNNEPMSEGWFYDPINGRLYVRINREPHKYTWQV
                     PKFNAAFMVDGRDWIWIEGFEMRYYGTEFGCGVCSKNSSHVVIRKNKIHHIQNPVFVE
                     WTGGEDRGNDTRIEYNEMYDTLTGGDWAWGAIKSTAMESMAIVLRGHTGAIVRGNTIH
                     HYFNGIYTSSSAALDNPGVAFDADIYNNYVYHIGDDAFEPEGTAVNHRFRNNKVDSTL
                     VGISLAPVTMGPVWIVRNTFTNFTARSVKWDRGSDGWVLMYHNTSFTTYTNTHSVELI
                     SAVKNSILRNNIFHANGYSVEAHNPGSTGHDWNYNDWFALNSNAPFMWEGISYATLNQ
                     WCRSTGLDCNGYEDSPGLSTDMTLLSNSPNIDRGVLIPGINDQFMGSAPDLGAYESVY
                     GTSPATPVATNTPFPLPTDTPTATPVYSSPVVTSITLADPTPNNAELVRFFVNFSEPV
                     NNVDVNDFMMTATGSISNYTILDAYPVGSAYAITVYTGVGDGTLRLDLVDNDSILSAT
                     SMHLGGVGIGNGNFSLGNVYVINKNAPFVTSILRADPSPSNAGSVRFSITFSESVSGV
                     DAADFVLSTSGLSEAGIASISGSGTAYMVTVNTGIGSGTLRLDLNDDDSVVDSAGAPV
                     GGIGVGNGNFAAGETYTIDRNIPLIETVSFSSDGAQDGWVMESKETSNKGGTLNSNAI
                     TFRVGDNAQDNQYRSILQFSTGALPDNAIITQMILTMQLETIVGTNPFTTHRNMWVDI
                     RQGAFGSFGPLQIGALQISDFQAPASLYNAGTIQNNAVGGWYWVNFDSGVFSLVNLKG
                     ATQFRLGFQLDDDDDRKDDYLSFFSGNYSVQSARPQLTIKYYIPK"
     gene            complement(3429..4064)
                     /locus_tag="PLG52_00095"
     CDS             complement(3429..4064)
                     /locus_tag="PLG52_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNB39865.1"
                     /translation="MTIPHETSPVYAAGAKLEEAKSAMILLHGRGASAQDILSLSTYL
                     NFPGMAYLAPQAEGSIWYPNRLIAPVESNEPYLSAAIAKIDGLVKHVEAGGIPVEKIF
                     IGGFSQGASLSSEFVIRNPRPYGGLLIFSGGYVWQSGKPRTAEGNLKGVPVFNGCSNV
                     DPHIPLERVKETTALLTSMGAEVNEQIYPNMDHTIMDEEIEQARALVEKRL"
     gene            complement(4061..5023)
                     /locus_tag="PLG52_00100"
     CDS             complement(4061..5023)
                     /locus_tag="PLG52_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HNB39866.1"
                     /translation="MNPIKGLHHVTAVARDPQRNVDFYRNVLGQRLVKRTVNFDSPDT
                     YHFYFADETGTPGSVLTFFAWPNMRRGVRGNGETNTVAYNVPVGSLSFWEKHLKQNGI
                     VPGNIQSRFNQNVLPFTDPDGMNLELVEVENLPAIQHWEDGPIPREYALHGFHSVTLW
                     VDEVEPTASLLTEGMGYRFAGAEEDRQRFKGDTNALGHIVDVVERPQKMPAGFGAGSI
                     HHIAFRVPTDDEQLKYQSILGEAGFRVSPVMDRSYFHSIYFREQGGVLFEIATDTPGF
                     MVDEPKDLLGIKLKLPAWVEPNRDEIEASLLPLTLNPIEKASLS"
     gene            complement(5123..5953)
                     /gene="speE"
                     /locus_tag="PLG52_00105"
     CDS             complement(5123..5953)
                     /gene="speE"
                     /locus_tag="PLG52_00105"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNB39867.1"
                     /translation="MSIWFTEELHPYYRKGIRVKRVLADEQTKYQHLQVVETEFFGNA
                     MILDGIIQLTERDNMGYHEMIVHVPMLAVEKPKRVLLIGGGDGGSLQQVLRYPSVEEV
                     SICELDQRVVDLSREHFAASFGNPWVDPRSKLLIRDAFGYLEENPGTFDVIISDTTDP
                     IGMAERLFSDEFYKLMVRALAPGGAAATQCEQPFFDTELIKTIYQTAKSLTKNPAYYY
                     TNVPTYPGGGIGFMYVSNTPWENGLKTPYPPGVNQYINPEIHKAAFALPEFFRKELYG
                     "
     gene            complement(6027..6965)
                     /locus_tag="PLG52_00110"
     CDS             complement(6027..6965)
                     /locus_tag="PLG52_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNB39868.1"
                     /translation="MSESKPPIYWISDYLTPDDILNHAVHKVILHKHTPFQEMWIVDC
                     GTYGKGLILDGIWQSSTGDDFLYHESLVQPACVNHGDPKRGLVLGGGEGATIRELLKW
                     KNMERVVMVDLDREVVEACKEHFPEMHQGAFDDPRVELVIQDALAYLDSSPSEWDVII
                     SDLTDPIEHGPSFMLFTKEYFAKAQKALRPGGYFLLQAGPVSPPSMDMHIRLVSTLKA
                     VFKYVHSYHIHVPMLGHPWGFILAGDTPIDVRPAPEMVDAILADKTNGKFKMFDGITL
                     LGLMQSPKHIRDAIAKETTVYTIEHPPVFSTQELPK"
     gene            complement(7039..7422)
                     /gene="speD"
                     /locus_tag="PLG52_00115"
     CDS             complement(7039..7422)
                     /gene="speD"
                     /locus_tag="PLG52_00115"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNB39869.1"
                     /translation="MNASLKLGEHYIFDLSDCNREILMDSEKAYSLFAQAVRDSGLTV
                     VDEGFYKFSPHGFTAFLLLAESHASLHAWPEHNYCAIDLFTCAIGQDFMPVLMRLKEA
                     FGADDFSVRKLDREASVETKMPIAM"
     gene            7735..8541
                     /locus_tag="PLG52_00120"
     CDS             7735..8541
                     /locus_tag="PLG52_00120"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB39870.1"
                     /translation="MENPLEHLHNETHQAWEANAEVWDKRMGDEGNDFFNLLCWPALA
                     SFLPSKSDAHILDIACGNGLTTRRLAEMGYQVTAFDFSANLIEYAKQRLDKHGSKTSL
                     HVIDATNEAQLLSLGKNKFDCALSNMALFDMANIEPLFQTLPQLLKPGGTFIFSITHP
                     CFNNASSMHVVEEMDDGEIKTIYAIKTSRYMTPYSMRGLALRNQPKPQVYFERPLQYY
                     LNLGFKNGFVLDGFEERAFPPETPQTTPLGWGGKFSEIPAALVIRMRLNS"
     gene            8574..>9751
                     /gene="hemW"
                     /locus_tag="PLG52_00125"
     CDS             8574..>9751
                     /gene="hemW"
                     /locus_tag="PLG52_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNB39871.1"
                     /translation="MPDTSLYLHIPFCKHRCAYCDFNTYAGQESLIPAYVEALIHEIE
                     FIGNQQPDFKVPTIFFGGGTPSLLSGPQFDSIMSALSSAFALSPTAEVTIEANPGTIS
                     PEKLEAIRKAGINRISFGVQSSVTEELRMLERSHDFFTVIEAVSTARKAGFDNLNLDL
                     IYALPEQTLTSWQTTVKRILELQPEHISAYALTLEHGTPFGKWADKGILPLPDPDLAA
                     DMYEWAMDFLVENDYVQYEISNWAKSTSEHMCKHNIQYWRSLPYLGLGAGAHGYAGGY
                     RYSNVLRIKTYIDRLSDTQSARLPFPLTPATVNQHKQTQRDDISDYMINNLRLVETGI
                     ADSDFRSRFGAGLLDVFPKEFEELIRNGLIEKKTSDGSEVFRLTHRGRLLGNQVFMRF
                     V"
     assembly_gap    9752..9851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9852..12544)
                     /gene="ppdK"
                     /locus_tag="PLG52_00130"
     CDS             complement(<9852..12544)
                     /gene="ppdK"
                     /locus_tag="PLG52_00130"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNB39872.1"
                     /translation="MSKWVYLYNEVKEVEKLAKGSWDGVKAIVGGKGAGLMDMTRANV
                     PVPPFFTVTTEACNAYQKAGKFPQGMWEQELKALKEIERKTGKKFGDPKNPLLVSCRS
                     GAKFSMPGMMNTILNIGLTDIVAEGMIKATNNDRFVWDSYRRVVEMFGTVVMDIEDEA
                     FEHPLEAYKEKKGYKLDIEMRAEDWKEVVGIYKQAYKKACGQEFPQDPYKQLSLATEA
                     VFRSWNGKRAVAYRRKEGISDALGTAVNICTMVFGNMGDDSATGVAFTRNPSTGEKKM
                     MGEYLLNAQGEDVVAGIRNTDAIENLKKYMPKAYAQFMEITARLEKHYTDMQDVEFTI
                     ERGKLWMLQTRVGKRTAKAAVKIAVDMANEGLINKDQAVQRVTPDQVDALLHPQFNDE
                     AMNQAEKEGKFLAKGVNASPGAAVGQIYFDADKAEEYAKEHKQDTIMVRPFTKPDDVH
                     GMIAAKGVLTSEGGATSHAAVVARQFGIPCVVGATAIKIDLEKRIMHVGEIVLNEGDW
                     VSVDGTTGKVFAGKIPTSEPSLEEQKELMTLLKWADDISARKNIREEVEPGYPTRGLQ
                     VWANADYPKDARRARSYGAVGIGLCRTEHMFFEPERLPIVQRMILAETSEGRTEALNL
                     LLPHQRKDFDGLFEAMDGYPVIIRLIDPPLHEFMPDEEKLLEEVITMRVKGETKDLAE
                     KEKLLESIKGMHESNPMMGLRGVRLSISMPEIVEMQVRAIFEAAADCTLRGIKVKPEI
                     MIPLTGTVKELDWIQPRLERIGKAVMQEKKIKFAYKFGSMIEIPRAAVTADHIATQAQ
                     FFSFGTNDLTQMTFGYSRDDAERNFLITYVQEGILVKNPFQTLDRNGVGKLMQRAIFE
                     GRAVRPDLEVGICGEHGGDPDSIEWCHLIGNNYVSCSPFRVP"
     gene            12748..13191
                     /locus_tag="PLG52_00135"
     CDS             12748..13191
                     /locus_tag="PLG52_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB39873.1"
                     /translation="MTPTAKILVIDDDAAITELMSMLLKTFGFEVLTTNIGEEGIRLA
                     REANPNVILLDLMMPDLDGWQVCKEIRKTSTVPILILSAINDPRMVASVLDVGADDFL
                     VKPVPSGVLVANIKKLIRRNTGALDMSKLKKTTASLQQANHALHP"
     gene            complement(13255..14019)
                     /locus_tag="PLG52_00140"
     CDS             complement(13255..14019)
                     /locus_tag="PLG52_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB39874.1"
                     /translation="MNKIKLNGIELAYERRGMGTPLMLIHGYPLDHSIWNDVAAQLEN
                     DFDLIIPDVRGFGESTTVDAPYSLSDIANDLAALLDSLGVGPTALAGHSMGGYIALAF
                     AKSFPQRVTGLALVSSQAASDTPERKEGRYKTAADVVQKGVQIVADAMTDKLSPNQSV
                     RDFVRPLIAKQSVAGVTGALKAMAERDDMTSFIASYAQPLVLIHGDADELIPLERARE
                     IKSLVSSAQLVELAGVGHMPMLEMPNETASALKKLK"
     gene            complement(14026..14562)
                     /locus_tag="PLG52_00145"
     CDS             complement(14026..14562)
                     /locus_tag="PLG52_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNB39875.1"
                     /translation="MNYVFFDLESQFLFDEVGGRDHADKLKVACAVTYSTAKNDFTVY
                     WENDVPALLAELKAATKVIGFNLISFDYRVLQPYANQVRLASIPTLDMLVDIHKNLGF
                     RVSLDNLASASLGATKTADGIQSVQWFRNGELDKVAEYCKADVDITRRVFEFGRDNGF
                     VYYKSKLGSKLKTPVKWK"
     gene            complement(14559..>15433)
                     /locus_tag="PLG52_00150"
     CDS             complement(14559..>15433)
                     /locus_tag="PLG52_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNB39876.1"
                     /translation="GVLQHLMPELLKMKGVTQSNPHIHEVWVHTLAVVDQLEDLLTSS
                     LRLDFDPEKTSDMFMGLISLKIGRYREQIAKHFANQLNPSRSHRSLLMFSALYHDVCK
                     PDTKTIDENGRIRFFDHDIKGAEVVADRARAFNLSNDEVERLHTIVRHHMRFHFFADR
                     LEREGQPPSRKAVYRFFRDSGAAGVDLILLALADLRGTKGNELTQVTWTAYLDIARIL
                     LENYWEHPEEVVNPPRLVDGNELMKELNLQPGKVVGVLLESIRENQAAGKIVDKEQAL
                     EFARVELTKGVNEA"
BASE COUNT         4059 a         3932 c         3468 g         3874 t
ORIGIN      
        1 ggaaaacctt tcagcttttt atgctggaag gttattaaga atttctcatc aaaatcactt
       61 gggaatgtaa tacttaatgg tcaactgtgg acgcgccgat tgaaccgagt aattcccgct
      121 gaaaaacgag aggtaatcat cttttcggtc atcgtcatca tccaattgaa aaccaaggcg
      181 aaactgagta gcccctttca aattaacaag tgagaaaacg cccgagtcaa aattaaccca
      241 ataccaccct cccacggcat tgttctgaat tgttccggca ttatacaaac tggcaggcgc
      301 ttgaaaatct gaaatctgca atgcaccgat ctgcaacgga ccaaaactac caaatgcccc
      361 ctgacgaata tccacccaca tattgcgatg ggtcgtaaag ggattcgttc caacaatagt
      421 ctctagttgc atcgtcaata tcatttgtgt aatgatggca ttatcaggca atgcccctgt
      481 ggagaactgt agaatggaac gatattgatt atcttgtgca ttatcaccta cacggaaggt
      541 gatcgcatta gagttcaagg ttccaccttt attgctggtc tctttcgact ccatcaccca
      601 accgtcttgg gctccgtctg aagagaaaga aacagtttca atcaacggga tattgcggtc
      661 aatcgtatag gtctcgccag cagcgaagtt cccattcccc actccaattc ctcccacagg
      721 ggcgccagcc gaatcaacaa cactatcatc gtcattcaag tcaagccgga gggttccact
      781 tccaatacct gtgttgacag tcaccatata ggcagtgcct gaaccgctta tggatgcaat
      841 gcccgcctca ctcaaacccg aagtggacag cacaaaatca gccgcatcca caccgctaac
      901 ggattcggag aatgtaatgc tgaagcgcac acttccagcg ttggaagggc ttgggtcagc
      961 acgcaggatg gaggtcacga atggggcgtt tttgttaatc acatagacat tgcccaatga
     1021 gaaattcccg ttaccaatcc caactccacc caaatgcata gacgttgcgc tcaagatgct
     1081 gtcgttatcc acaagatcaa gacgcagggt tccatcccct acaccggtat acactgtaat
     1141 cgcatacgcg cttccgacag ggtacgcatc aaggatggtg taatttgaaa tggaacctgt
     1201 tgcggtcatc ataaaatcat tgacatctac attgttgacc ggctctgaaa agttcacaaa
     1261 aaagcgtaca agctctgcat tatttggtgt tgggtctgct aaagtaatgg aagtaaccac
     1321 cggcgacgaa taaacaggcg tcgcggttgg agtatcagtt ggcaacggaa aaggtgtgtt
     1381 ggttgcgaca ggtgtagcgg gagatgttcc atacacagac tcatatgcgc caagatcagg
     1441 cgcactgccc ataaattgat cgttaattcc aggaatcaga acgccacggt caatgttggg
     1501 gctgtttgat aacaaagtca tatctgtgga taagcccggt gaatcttcat agccattgca
     1561 atctaaccct gttgaacggc accactgatt caaagtggca tatgaaatgc cttcccacat
     1621 aaaaggagca tttgaattca atgcaaacca atcgttgtaa ttccaatcat gacccgttga
     1681 accggggttg tgcgcctcta cagaataacc gtttgcatgg aaaatgttat tccgcaagat
     1741 tgagtttttc acagcgctaa tcagctcaac tgaatgggta ttggtataag tcgtaaaact
     1801 agtgttgtga tacatcagta cccaaccatc tgagccgcga tcccacttca cactgcgagc
     1861 cgtaaagttc gtgaaggtgt tccgtacaat ccaaactgga cccatggtta caggggcaag
     1921 cgaaatacca actagagtcg aatccacttt gttatttcga aaacgatgat taacggctgt
     1981 cccttcaggc tcaaacgcat catccccgat atgatagaca tagttgttgt aaatatctgc
     2041 atcaaaggca acaccagggt tgtctaatgc agccgaggag ctggtgtaaa tgccattgaa
     2101 ataatggtgg atggtattgc cacgtacaat ggcgcccgta tgcccccgca agacaattgc
     2161 catagactcc attgccgtac tcttaatagc gccccatgcc caatcgccac cggtaagtgt
     2221 gtcgtacatt tcgttgtatt cgatacgagt atcattgccg cggtcttctc cgcctgtcca
     2281 ttctacaaag accgggtttt gaatatgatg aattttgttc ttgcgaatca caacatgcga
     2341 agaattttta gaacaaacgc cacagccaaa ttctgtgcca tagtagcgca tctcaaaacc
     2401 ttcgatccaa atccaatcac gtccatctac cataaacgcg gcattgaact ttggaacttg
     2461 ccatgtatat ttatgcggct cacgattgat gcgcacatac aaacgaccat tgatagggtc
     2521 ataaaaccaa ccttcactca tcggctcatt gttatgtccg cgttggtcgt aaaggtcact
     2581 caaaaaatca tattgataaa aacgcaatcc atcccgcgcc agatacttaa tccccgggct
     2641 aatcttcgta aaccagacat tggctttgcc ttcaacagga ttccaatccg ttgctaaaac
     2701 cgaagcggac ccatctaaaa tcgcgcctcc gccctgtgcc ttgacctgaa tccagcttcc
     2761 agccgttccc gaagccggga atgtgaccga ctcactgtaa atcccatctg ccaccaaaac
     2821 ttgcgtgcct ggtgctgcac gattgacccc ttcctgtatc gtacgaaatg gcgcagaggc
     2881 agacccatcc ccacttgcct gtgcagatgc atttacataa ataatattcg tgggcgcaaa
     2941 aagtaactca tcggcttgag tggtaaacga accagaaact tctgttacac catcagaaac
     3001 gcgcacttca taagaggtag atgcagccaa gttaaacaag ctaccaatca aacgtccgtc
     3061 tttgatgcgc aaaagggcat gccctggctg ccagtttaca tcccctgccc ggcggtaaga
     3121 caactgagcc gtggccggca gccccgttcc gctgacgaca actcccgccg tttcaatatt
     3181 gggcacaaaa ctaattgtcc ccaaagcctg ttctgggcgt gcactgaccc atccactcgg
     3241 cacaagcagt gataacagga gcgaaagggt tacacccagc actataaatt tcgatatact
     3301 accatgcgtg ttcataaaaa ccatttacag ggatggaaac aaaaccctgt ctgttttcac
     3361 tatagcacgc acctataaaa atacaattct ggtaccatga caaaaaacgc ccctttcggg
     3421 gcgatttctt ataacctttt ctcaaccaag gctcttgctt gctcgatctc ttcgtccata
     3481 atcgtgtggt ccatgtttgg gtaaatttgc tcgtttactt ctgcccccat ggaagtcagg
     3541 agtgccgtgg tctctttcac tcgctccaac ggaatatgag gatccacatt cgaacagccg
     3601 ttgaaaaccg gtacaccttt caagttgcct tccgcagtgc ggggtttccc gctttgccaa
     3661 acatatccgc cgctaaagat caacagccca ccatacgggc gcggattgcg aatcacaaat
     3721 tcgctggaaa gactggcgcc ttgagagaaa ccgccaataa atattttttc gacaggaata
     3781 ccgcccgctt caacatgttt taccaaccca tcgatcttcg cgatggcggc tgaaaggtac
     3841 ggttcattgg attcaacagg cgcgattaaa cggttcgggt accaaatgga tccttccgct
     3901 tggggggcga gatacgccat gcccggaaag ttcaagtatg tggaaaggga cagaatatct
     3961 tgtgccgatg cgccacgtcc atgaaggagt atcatcgcag atttggcttc ttccagtttc
     4021 gcacccgctg catagacagg ggatgtttcg tgaggaatgg tcatgaaagg ctcgctttct
     4081 caatagggtt aagggttaat ggcagtaagc tggcttcgat ctcatctcga ttgggttcta
     4141 cccaggcggg cagtttcaac tttatgccca gcaaatcctt gggttcatcg accatgaaac
     4201 cgggtgtgtc ggttgcgatc tcaaacaaca caccgccttg ctcccgaaaa taaatggaat
     4261 ggaaatagct gcgatccatt accgggctta cgcgaaaccc cgcctcaccc aagatggact
     4321 gatatttcag ttgttcatca tccgtgggca cgcgaaaagc aatatgatgg atggaaccag
     4381 ccccaaaccc tgcaggcatc ttttgcgggc gctcaaccac atccacaatg tgacctaggg
     4441 cattcgtatc acccttaaag cgttggcggt cttcctctgc cccggcgaag cgatagccca
     4501 tgccttcggt caagagcgaa gcggttggtt caacttcatc cacccacaaa gtgacgctat
     4561 gaaatccatg caaagcatat tcacgtggga tgggtccatc ttcccaatgc tgaatggcgg
     4621 gcaaattttc cacttccacc aattccaggt tcatgccatc ggggtccgta aaaggaagaa
     4681 cattctgatt aaagcggctt tgaatattgc cggggacaat gccgttttgt ttcaggtgtt
     4741 tttcccaaaa ggaaagtgaa ccaaccggga cgttatatgc aacggtattg gtttcgccat
     4801 tgccgcgcac gccgcgccgc atattgggcc aagcgaagaa ggtcaataca ctgccgggtg
     4861 tgccggtttc atctgcaaaa tagaagtgat aggtatcggg cgagtcgaag ttaactgtgc
     4921 gcttgaccaa gcgttgccct aaaacatttc gataaaaatc cacatttcgt tgtgggtcac
     4981 gtgccacggc tgtgacatga tgtaaacctt taatagggtt catatttctc tcctattgcc
     5041 tagacgcact ctatcgcaaa aatatccgtt tgaatttcgt atgaaaagcc tccaaagtta
     5101 attttggagg cttggtctta ttttacccgt acaattcttt gcggaagaat tctggcaaag
     5161 caaacgccgc tttgtggatc tctgggttga tgtattggtt cacaccgggt ggatagggcg
     5221 ttttcaaacc gttttcccaa ggagtgttcg aaacgtacat gaaccctatc ccaccgccgg
     5281 ggtatgtggg gacgttggtg tagtaatagg cgggattctt ggtgagtgac ttggcggttt
     5341 gataaatggt tttgatcaac tcggtgtcaa agaaaggctg ctcgcattga gtcgcagcgg
     5401 caccaccagg agctagggca cgtaccatca gcttgtaaaa ttcatcggaa aagagacgct
     5461 ctgccatgcc aataggatcg gtggtatctg agatgatgac atcgaaggtg ccgggatttt
     5521 cttctaggta gccaaaggcg tcccgaatga gcaacttgga gcgtggatca acccatgggt
     5581 tcccgaaaga ggcggcgaag tgttcgcgtg agaggtccac gacgcgttga tccagttcgc
     5641 agatagaaac ttcctcgacg gaaggatatc gcaagacttg ttgaagcgag ccaccgtcgc
     5701 cgccgccgat aagcaacact cgcttgggct tctccaccgc cagcatgggc acgtggacga
     5761 tcatttcatg atagcccatg ttgtcgcgct cggtcagttg gatgatgcca tcgaggatca
     5821 tggcgttgcc aaaaaattcg gtttcgacaa cttgcaagtg ttgatatttt gtctgttcat
     5881 cggcaagcac gcgtttgaca cggatgcctt tgcgatagta agggtgtaac tcttcagtga
     5941 accaaatgct cattcgttat cctttcaagt aaagaccgcc gaccattctg ttgtcggcgg
     6001 tcttgtaacg gcttaggtct ttactactat ttcggtaatt cctgcgtgct gaatacgggc
     6061 ggatgttcaa tggtgtaaac tgtggtctct tttgcaatcg catcgcggat atgctttggc
     6121 gattgcatca gaccgagcaa agtgatgccg tcgaacattt tgaatttgcc gttggtcttg
     6181 tcggcaagaa tggcatcgac catctccggt gcagggcgga cgtcgatggg cgtatcacca
     6241 gcaaggataa agccccaagg atgcccgagc ataggcacat ggatatgata ggaatgcaca
     6301 tacttgaaaa ccgccttgag cgtagaaacg aggcggatat gcatgtccat tgaaggcggc
     6361 gaaacgggac ccgcttgaag aaggaagtaa ccgccggggc gaagtgcttt ttgcgcctta
     6421 gcaaaatatt ccttggtgaa caacatgaaa gacggtccat gttcgatggg gtcggtcaag
     6481 tcggagatga tgacatccca ttcagaggga gagctatcga gataggcaag tgcatcttga
     6541 atgaccaatt ccacgcgtgg gtcgtcaaac gcaccctgat gcatttcagg gaaatgctcc
     6601 ttgcaggctt caaccacttc acggtcaagg tcgaccatga cgacgcgttc catgtttttc
     6661 cacttgagca attcacggat ggtcgcacct tccccgccgc caaggacgag tccgcgcttg
     6721 gggtcaccgt gattgacaca ggcaggttga acaagcgact cgtgatagag gaaatcatcg
     6781 ccagtggatg attgccaaat gccatcgagg atgagaccct tgccgtaggt gccacagtcc
     6841 acgatccaca tttcctggaa tggggtgtgt ttatgcagga tgaccttatg aactgcatgg
     6901 ttcaaaatat catcaggggt taggtagtca gagatccaat agatcggggg ttttgattca
     6961 gacattcttt ttccttggtg aatgaaaata atttagatct acggagtata tcaaaacgat
     7021 agaagataga ccacttaact acattgcgat cggcattttg gtttcaacag acgcttcacg
     7081 gtcgagcttg cgcacagaga agtcatccgc gccaaaggct tctttgaggc gcatcaaaac
     7141 aggcataaag tcttgcccaa tagcacaggt aaataagtcg atggcgcaat aattatgctc
     7201 gggccaggca tgcaggctag catgcgactc cgcaaggaga agaaaagcgg taaagccatg
     7261 cgggctaaat ttataaaatc cttcgtctac aacggtcaga ccgctatcgc gcacagcctg
     7321 agcaaacaac gagtaagcct tctcgctatc catgaggatc tcacggttgc agtcggagag
     7381 atcgaaaatg taatgctctc ccaatttcaa actagcgttc attcacacct ccggtgttaa
     7441 aaaattaaag aacccaccca agaacctttc ccaaacgatc cgcaggatgt ttccctgcgt
     7501 aacggttgag accgtcctta agtagactct gttgcgccga tttatactat ggactggtgt
     7561 agtgtcaatc tttttcacca gtgaatattt ttcattgtca aagcctgttg aaatcaaaat
     7621 catcattgca agcaagatac aggtcataat catgactcaa agcaagccta cataaacaat
     7681 ttgattcacg tataaaataa gccgcaagac catttcaaat ctttaagagg ttcaatggaa
     7741 aacccgctgg aacatttgca taatgaaacc caccaagcat gggaagccaa tgccgaggta
     7801 tgggacaaac gcatgggcga cgaaggcaat gattttttca atctgctgtg ctggcccgct
     7861 ctcgcctcct tcctaccctc aaaatcagat gcccacattc tggatattgc ctgtggaaat
     7921 ggcttaacca cccggcgact tgccgaaatg ggttaccaag tcaccgcttt cgacttttcc
     7981 gccaatctaa tcgaatatgc caaacaacgg cttgacaagc acggctcaaa aacatccctg
     8041 catgttatag acgccacaaa cgaagctcaa cttctctctc ttggcaagaa taaattcgac
     8101 tgtgcgctct ccaatatggc gctcttcgac atggcaaata tcgaaccgct ctttcaaacc
     8161 ctaccgcaat tgcttaaacc gggtggaact tttattttct ccatcaccca tccctgcttt
     8221 aataatgctt cgtccatgca tgtcgtagaa gaaatggacg atggcgagat caaaaccatc
     8281 tacgccatca aaacctcgcg ctatatgaca ccctactcca tgcgcggact cgctcttcgc
     8341 aaccaaccca aaccgcaagt ctattttgaa cgtccgttgc aatactactt aaaccttggc
     8401 ttcaaaaatg gcttcgtcct cgatggcttc gaagaacgtg cgtttccacc agaaacaccg
     8461 caaaccacac cgcttggctg gggcggcaag ttcagcgaaa tcccagccgc actcgtcata
     8521 cgcatgcggc taaactctta gccttcaagg cggttatact caacccatat caaatgcccg
     8581 acacctccct ctacctccac atccctttct gcaaacaccg ctgcgcctat tgcgacttca
     8641 acacctatgc cgggcaagaa tctttgattc ccgcttacgt cgaagcctta atccatgaga
     8701 ttgaatttat cggaaatcaa caaccagatt tcaaagtacc aaccatattt tttggcggag
     8761 gaaccccctc gcttctttct ggacctcaat ttgattccat tatgtcagct ttatcttccg
     8821 cctttgcctt gtcccccacg gcagaggtga ccatcgaagc caaccctggc accatctcgc
     8881 cagaaaaact ggaagccatt cgaaaagcag gcatcaaccg cattagcttc ggtgtgcaat
     8941 cttctgtcac agaagaattg cgcatgcttg aacgctctca tgattttttc accgtcatcg
     9001 aagccgtatc cactgcccga aaagccggtt tcgataatct caaccttgac ctaatctacg
     9061 ccttgccaga acaaaccctc acatcttggc aaactactgt caaacgcatt ttggaattac
     9121 agcccgaaca catctctgct tatgcactca cccttgagca tggcactccc ttcggcaaat
     9181 gggcagacaa aggcatcctc ccccttccag atcccgacct cgccgccgat atgtatgaat
     9241 gggctatgga ttttctagtt gagaatgact atgtccaata cgagatttca aactgggcaa
     9301 aatcaacttc tgaacatatg tgtaaacata atattcaata ttggcgctca cttccatacc
     9361 tcggtcttgg tgctggcgcg catggctatg caggcggcta tcgctattcg aatgttttac
     9421 gcatcaaaac gtatatagac cggttaagtg acacacaatc tgcccgtcta ccttttcctc
     9481 ttacaccagc tacagttaac cagcacaaac aaactcaacg cgacgatatc tctgattaca
     9541 tgatcaataa tttacggctg gtagaaaccg gtatcgctga ttcagatttc aggtccaggt
     9601 ttggagccgg gcttttagat gtctttccaa aagagttcga ggagcttatc cgcaatgggc
     9661 tcatcgaaaa aaagacctcc gatggctcgg aggtctttag gcttactcat cgcggaaggc
     9721 ttttggggaa tcaagtcttc atgcggtttg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nggcacacgg aagggggaac aggaaacata gttgttacca atgaggtgac
     9901 accattcgat agagtcgggg tcgccaccgt gttcaccaca gataccaact tcaagatcag
     9961 gtcggacggc acgtccttca aagatggcgc gctgcatcag cttgccaaca ccgttgcgat
    10021 cgagagtctg gaagggattc ttgacgagga taccttcctg cacataagta atgaggaagt
    10081 tgcgttcggc atcgtcacgc gaataaccga aggtcatttg tgtaaggtca ttcgtaccaa
    10141 aggagaagaa ctgcgcctgg gtggcaatgt ggtctgctgt aacagcagca cgcggaatct
    10201 cgatcatgga gccaaattta taggcgaact tgatcttctt ttcctgcatc acggctttac
    10261 cgatgcgttc gaggcgcggc tggatccagt ctaactcttt cacggttccg gtcaacggga
    10321 tcatgatctc aggcttgacc ttgataccgc gcagggtgca gtcggcagcg gcttcaaaga
    10381 tggcacgcac ttgcatttcc acgatctcgg gcatcgagat gctcaaacgc acgccgcgca
    10441 aacccatcat cgggttggat tcgtgcatgc ccttgatcga ttcaagcagt ttttcttttt
    10501 ctgccaagtc tttcgtctca cccttcactc gcatggtaat aacttcttca aggagttttt
    10561 cttcgtcggg catgaattca tgcaaaggtg ggtcgatcaa acgaataata acggggtaac
    10621 cgtccatcgc ttcgaagaga ccgtcaaagt ccttgcgctg atgcggtaag agcaagttga
    10681 gcgcttcagt acgtccttcg ctggtttcag cgagaatcat gcgttgcaca atcggcaagc
    10741 gttcaggttc aaagaacata tgctccgtgc ggcacaaacc aatacccaca gctccataag
    10801 agcgagcacg gcgagcatct tttggataat cagcatttgc ccaaacttgt aaaccacggg
    10861 ttgggtaacc gggctccact tcttcgcgaa tgtttttgcg agcggaaata tcatctgccc
    10921 acttgagcag ggtcattaat tctttctgtt cttcaagaga aggttcagag gttgggatct
    10981 ttccagcaaa gactttacca gttgtgccgt ctacagagac ccaatcacct tcgttgagca
    11041 caatttcacc cacatgcata atgcgctttt caaggtcgat cttgatggct gtagcaccca
    11101 ccacgcatgg gataccaaat tggcgagcca caacggcggc gtgagaagta gcgccacctt
    11161 cactggtcaa cacacccttg gcagcgatca taccgtggac atcatctggc ttggtaaatg
    11221 gacgtaccat gatggtatct tgcttgtgtt ccttggcata ttcttcagct ttatcggcat
    11281 caaaataaat ttgaccaaca gccgcacctg gagaagcatt cacacccttg gcaaggaact
    11341 taccttcttt ttcagcctga ttcatagctt catcgttaaa ttgcgggtga agtaaggcat
    11401 ccacctgatc tggggtgaca cgttgaaccg cctgatcttt attgatcaga ccttcatttg
    11461 ccatatccac agcgatcttc acagcagctt tggcagtgcg tttgcccaca cgagtctgaa
    11521 gcatccacaa ctttccgcgt tcaatggtga attccacatc ctgcatatcg gtgtagtgct
    11581 tttcaaggcg ggcggtaatt tccatgaact gagcatatgc cttgggcata tacttcttga
    11641 ggttttcaat agcatccgta ttgcggatac cagctaccac atcttcacct tgtgcattaa
    11701 gcaggtactc acccatcatc ttcttttcgc cagtggatgg gttacgcgtg aatgccacac
    11761 cggtagcaga gtcatcgccc atgttcccga agaccatcgt acaaatattg acggcggttc
    11821 cgagcgcatc agagatacct tccttgcggc ggtatgcaac agcacgtttt ccattccagg
    11881 agcggaatac agcttcagtt gctaatgaga gttgtttata aggatcttgg gggaattcct
    11941 gaccacaggc tttcttgtac gcttgcttat aaatccccac aacttctttc caatcttccg
    12001 ccctcatttc aatatcgagt ttataacctt ttttctcttt atatgcttcc aatggatgtt
    12061 caaaggcttc gtcttcgatg tccatcacga cggttccgaa catttcaacc acacgtcgat
    12121 atgagtccca cacaaaccgg tcgttgttcg ttgccttgat cataccttct gctacgatat
    12181 cggtcagacc gatattgaga atggtattca tcatgcccgg cattgagaac ttagcacctg
    12241 agcgacacga gaccaaaagc ggatttttcg gatcaccgaa ttttttgccg gtcttgcgct
    12301 cgatctcttt cagcgctttc aattcctgct cccacattcc ttgcgggaat ttcccagctt
    12361 tttgatatgc attgcacgct tctgtagtaa cggtaaagaa aggtggaacc ggcacattcg
    12421 cgcgagtcat atccattaag cccgcacctt tgccgccgac gatggctttg acaccatccc
    12481 atgagccttt ggctaatttt tcaacttcct tcacctcatt atagagataa acccatttag
    12541 acatttcgac ctcctgatcg agtaatattg tcactctata tcttgattgt gaaatattgt
    12601 accaaaaaca gtccaattag cacctgacaa aaatcatgct aagagtggat gtcaatcagg
    12661 gaaattacta attcttattc aaaagaacct gttaatacat tgcaaagtac attgtcagtc
    12721 aaatcaggga taatgtgcct agatattatg acacctacag caaaaatcct tgtgatcgat
    12781 gatgatgctg ccatcacaga gttgatgagc atgttactaa aaacctttgg gtttgaagtg
    12841 ctcacaacca acattggcga agaaggcatc cgccttgcgc gtgaagccaa cccaaacgtc
    12901 atcctactcg atctaatgat gcctgattta gacggatggc aggtttgcaa agaaattcgc
    12961 aaaacaagta ccgtgccgat cctgattctt tctgcaatca acgatccacg catggttgcg
    13021 agcgttttgg atgttggcgc agacgatttc ctcgtcaaac cggtgcccag cggcgtgttg
    13081 gttgcgaata tcaagaaatt gattcgacgc aacacaggcg cactggatat gtccaagtta
    13141 aaaaaaacga cggcttccct ccaacaagct aatcacgcgc ttcaccccta gcccgaagct
    13201 ggttctacaa aataaaaatg cccatccccg ttagctggat gggcattttc tttgctattt
    13261 gagctttttc aacgccgaag ccgtttcgtt tggcatttcc agcatgggca tatgccccac
    13321 cccagccaac tccaccaact gcgcagacga gacaagtgac ttaatctccc gcgcacgttc
    13381 gagcggtatc aactcatctg catctccgtg aatcaacaca agaggctgag cataagatgc
    13441 tatgaacgaa gtcatatcgt cacgctccgc catggctttg agtgcccccg tcacaccagc
    13501 cacgctttgc ttcgcaatca acgggcggac aaaatctcgc acagactgat tgggcgaaag
    13561 tttatccgtc atcgcatcgg cgacgatctg aactcctttt tgaacaacat ctgccgccgt
    13621 tttatatcta ccttctttac gttcaggggt atctgaggct gcttgagagg aaaccaaagc
    13681 caaaccggtc acacgctgtg gaaaagactt cgcaaatgcg agagcgatat aacctcccat
    13741 ggaatgacca gccaaggctg ttggacccac tccgagcgag tccagaagcg cggcaaggtc
    13801 gttggcaatg tctgagagag agtaaggtgc gtcaacagta gttgattcgc cgaatccgcg
    13861 cacatcgggg atgatcaggt cgaaatcatt ttcgagttga gcagcgacat cattccaaat
    13921 cgaatggtcc aaaggatagc cgtggatgag catcaaaggg gttcccatgc cgcgccgttc
    13981 gtatgccagt tcaataccgt taagtttgat tttgttcata tcgctttact tccacttgac
    14041 cggggttttc agcttgctgc ccagcttaga cttgtaatac acgaagccat tatcgcgccc
    14101 gaattcaaag acgcggcgag tgatatctac atcggctttg cagtattcag caactttatc
    14161 cagctcgccg ttacgaaacc actgcacaga ctgaatgcca tctgctgttt tggttgcccc
    14221 aagagatgca ctcgccaaat tatcaaggct cactctaaac cctaaattct tgtggatatc
    14281 taccaacata tccaaagttg gaatggatgc gagccgaacc tgatttgcat acggctgcaa
    14341 gactcgataa tcaaagctaa tgaggttgaa cccaatgacc ttggtagcgg ctttcaactc
    14401 cgccagcaaa gcagggacat cattctccca atacactgta aagtcatttt ttgcagtgga
    14461 atatgtgacc gcgcatgcga ccttcaattt atcggcatga tcacgcccgc ctacttcatc
    14521 aaaaagaaat tgagactcga ggtcgaagaa tacatagttc atgcttcatt cactcctttc
    14581 gtaagttcta cacgtgcaaa ctctaaagct tgttctttat ctacgatttt gccagcagct
    14641 tgattctcac gaattgactc aagcaacacg cccacaactt ttcctggctg aaggttcagt
    14701 tctttcatca actcattccc atccactaac cgcggcgggt tgacgacttc ttcagggtgt
    14761 tcccaataat tctccagcaa aatacgagca atatcgagat acgccgtcca agtcacttgg
    14821 gtcagttcgt tgcctttggt tccgcgcaaa tctgccaagg cgaggaggat taaatcgacc
    14881 ccggcggcgc cgctatcgcg aaagaagcga tacacagctt tgcgagaagg cggctgtcct
    14941 tccctttcca agcgatcggc aaagaaatga aaacgcatat gatgccgaac gatggtatgc
    15001 aagcgctcaa cttcgtcatt gcttaagttg aaagcccgag cgcggtctgc aacgacttct
    15061 gcccctttga tgtcgtggtc aaagaagcgg atgcgcccat tttcgtcgat agtttttgtg
    15121 tctggcttgc agacgtcatg atataaagca gaaaacatca acaacgaacg atgggagcgg
    15181 cttgggttga gttgattggc aaaatgtttg gcgatttgtt cgcggtaacg cccgatcttc
    15241 aaactaatca gccccataaa catatcgctg gtcttttcgg ggtcaaagtc taaccgtaga
    15301 gacgaggtga gcaaatcttc aagctggtct accacagcga gagtgtgtac ccacacttcg
    15361 tggatgtgcg gattggattg ggtaacgccc ttcattttca acaattcagg cataaggtgt
    15421 tgtaaaacgc cca
//
LOCUS       DAOQNJ010000003        15023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_1589_length_15023_cov_8.026456, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000003 DAOQNJ010000000
VERSION     DAOQNJ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15023
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1589_length_15023_cov_8.026456"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLG52_00155"
     CDS             <1..342
                     /locus_tag="PLG52_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HNB39877.1"
                     /translation="SHHMIVVGNDDESMAVAANELAKCGGGQIVVMDGKVIGQVEFPI
                     AGLMSNERADVVAKKAATVLEGFKMCGSELNNPNMQLSLMALVVIPELRISDKGLVDV
                     TQFDFIPVLED"
     gene            358..1074
                     /locus_tag="PLG52_00160"
     CDS             358..1074
                     /locus_tag="PLG52_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB39878.1"
                     /translation="MANFPFQRLQTDLAALINKTPAGQRLPSEPELAKQLGVSRATLR
                     EAMRSFETQGLIRRRQGSGTFVVGKVQALDSGLEVLESLETLAKRIGLEVSVSDLSIE
                     AIAADADLAASLNVTVGAKLTRVRRVIRAEGRPVAYLVDTLPEDFVHAKDVPSDFHGS
                     VLDFLLNRGDLLASSRANVSAIGATADVAKPLEIQRGDVLLHFNSQLFDSEGKVVDYS
                     LSYFIPGYFRFHIVRRVGNG"
     gene            1128..2327
                     /locus_tag="PLG52_00165"
     CDS             1128..2327
                     /locus_tag="PLG52_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNB39879.1"
                     /translation="MTDTTKEIQKRVQSSREDIIEFMRDIVAIPSMDSQIGPVGERIQ
                     AEMKKLGFDEVRFDKMGNTIGRIGNGKRVMVYDSHIDTVGIGDPSSWQWDPFKGKIED
                     GILYARGACDEKGSTPGMIYGLAIARDLGLLDGWTVYYFGNMEEWCDGIAPNTFAEVD
                     PGIKPDFVVIGEPTKMLVYRGHKGRLEMKVTAKGKSAHAASNHLGDNAIYKLLPVIAG
                     IRDLEPKLGDHEFLGHGKITVSDMHVKTPSINAVPDEAVIFIDRRMTFGETKEAVKKQ
                     VEDLIPAEFKDTVKLEELFYDEPSYTGFVFPVDKYFPAWAYEENHPLVQAGQEARKFI
                     GLPEAKPSKWNFSTNGIYWAGKAGIPSIGFGPGDEETAHTVNDSVSLEDMVKATEFYA
                     ILPSLIK"
     gene            2341..2880
                     /locus_tag="PLG52_00170"
     CDS             2341..2880
                     /locus_tag="PLG52_00170"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNB39880.1"
                     /translation="MKILEVRRHSIRHAGGDHLSQAGVALARNVGQNIGPFDFVSTST
                     LPRAFDTAIAMGFAVNEQNPLMNTYGGAVEREAPWPMPFFHYSEIVKQAGAAAKYAHD
                     LMDYYLGILNHIPQGGSALVVNHGGVVELGVVACLPDADFSAWGDAVEYCEGARLFWD
                     GGKFVNGEVLRLMYNKESL"
     gene            2877..3851
                     /locus_tag="PLG52_00175"
     CDS             2877..3851
                     /locus_tag="PLG52_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB39881.1"
                     /translation="MRKALPVLFILTMLLLACGSNTAKPKVALMLARGGLGDKAFNDS
                     ANEGLQKAAQELNAEVTTFDYAEDRVEANIRQAAEDGYSLIIGLGSENSQAIATVAKE
                     FPETKFAIIDATAEGDNVTSVTFSELEGDFLAGALSALMSENGKVGYLGGADVLVIRR
                     IQYGFEQGVKYINPDAEIVVQYIGGKDDFSGFAKPDEAQTIADQMYSDGVDIIYAAAG
                     GSTLGAIKAAESAGKPIVTTGSDQRYLAPNVVATSRTKNMNEAVFMLIQKFVDDSLPS
                     GTIQLDYKSGGIGLAPLADFVPASVSEAFLFVKSDIEDGAIKVEPFTE"
     gene            3898..4878
                     /locus_tag="PLG52_00180"
     CDS             3898..4878
                     /locus_tag="PLG52_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNB39882.1"
                     /translation="MQTNFRGRDFIGDLDFSKEEVETVLDVAWDLKRKRALGESHALL
                     RDKTLAMLFFFTSTRTRGSFEAGMAQLGGHAAFIDSETTQISHGDTAKEIGEIFGRYF
                     DGIAIRQCDWNFGNQYINDVAKASRVPILNMQCDVYHPFQCLADLMTIQEKKGRDLKK
                     KKVVVSWAYAASYSKPISVPQSLILQMTRFGCDVVLAHPPEFKLMPEIMQQAKDNAKK
                     YGVGFDVTDNMDEAFKDADVVYPKSWGPMLTTTGKEEGKALIEKYKDWITDERRMKLT
                     KDDSIYMHCLPADRGLEVTDGVIDGPHSVVYDEAENRLHAQKAVMALTMS"
     gene            4942..5895
                     /gene="arcC"
                     /locus_tag="PLG52_00185"
     CDS             4942..5895
                     /gene="arcC"
                     /locus_tag="PLG52_00185"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNB39883.1"
                     /translation="MTNLAVIAVGGNSLIIDDKKVTVESQYQAAKQTCVHIADMIEQG
                     WDVAIGHGNGPQVGYILRRSEIAAKTAGMHEVPLDVCGADSQGAIGYAIQQNLQNILY
                     QRGIKKKVVTVITQTLVDKTDPAFSKPAKPIGSFMDKEEAERRQQEQGWSVVEDAGRG
                     WRRVVASPLPKEIVELETVQTLLEKGIITITVGGGGIPVVDPGDGNYEGVAAVVDKDY
                     ASSLLARSIGANLFVISTAVEKVAINFGKPEQKWLDQITLAEAKAYLAEGIHFAKGSM
                     APKIQAIIWYLEAMPNGKALITNPENIGRALKGETGTWIVP"
     gene            5949..7964
                     /locus_tag="PLG52_00190"
     CDS             5949..7964
                     /locus_tag="PLG52_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39884.1"
                     /translation="MKKLTDRLNNIQFTPRSTVWFLLLIAALTYTLFFWQRGFYWDEF
                     PWAWIYYRLGPAMLTKTFTTSRPFWGMIYQLTMPVIGPNPWIWQLLAIVLRWVTAVLL
                     WKILRALYPEHPKPALWASLFFLVFPGMGQQFIAFMYSHFYIVLSALLLSMYLAIRAI
                     QDEKRRWLYFSISYVLAFVNFITMEYFYFIEFGLLIVFYQLLPGTFKERAKKTLLYFL
                     PYLAIFIGITVWRTFFFEFQNASYQFVLLDKLKADVFSGLLYFLNQILVSFWRTVFEA
                     WILPFLTIGTTGFGPLTLSLMLVLLFSSILLTGVYLFAFKAESANGLDFAKRAGLVGL
                     AFWGLSGGSVWMIGNLPQFNFSLDRFMLPFMLGSSILLASLIALIKSPRWQMILVALL
                     IGFAGSRHFRLEEIYRSDWLTQQELFTQMTERIPGLKKGTILISNDIPVTYYSDNSLT
                     GPLNWIYSPQGEMNVILYFASIRVGKTLPSVEPGQQHQLYYIGPTFYGNTDNILVVNF
                     EPPGCFRVVDPEVEVDNRLLSPAVRDVAQYSNQSVILFEKQNQLPGQLYGGAPEADWC
                     HYFEQAELARQQGDWETVTHLGDAAFSLSDYPNDPLERFVYIEGYAHQSNWKKAVELS
                     KETYKISKTYVGPLLCRLWGRIERETEASSEQVAALELVRSEFGCLP"
     gene            8096..9367
                     /locus_tag="PLG52_00195"
     CDS             8096..9367
                     /locus_tag="PLG52_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB39885.1"
                     /translation="MRNLFARLAVLALALMLVLSACGGQPAATEEAPAATDAAPAATD
                     APAATDAAPAAEPLTFGMLLVGPYNDNGWSQAHYEAGKYVEEQLGAKMLYLDKVNPAD
                     RPGTTPDQLAEELVAQGATLIIFNSDDMKDASTTFAKNHPDVYVIMASGDQVWSDGKA
                     FEAMPNLTNIMGRMEYGKMMAGCAAALASETGKIGYLGPLINDETRRLAASAYLGAKY
                     CYEKAGNDPASLEFKVTWIGFWFNIPGVTLDPVQVSNDFYTTDYDVVISGIDNPVNLG
                     EAAKLKAEGKNVSGVAYDYVAACEAAPDVCIGVPYFNWGPEYVKAISSAQDGSWQSQF
                     IWAGPDWADINNPDTSTVGFVKGPALADDDAATLDAFIAELAGGLNLWSGPINLQDGS
                     AYLADGEVATDQQIWYLPQLLEGMDGLSAAE"
     gene            9444..10937
                     /locus_tag="PLG52_00200"
     CDS             9444..10937
                     /locus_tag="PLG52_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB39886.1"
                     /translation="MKVELKNIHKHFGKTYANKGINLNIPSGSIQGILGENGAGKSTL
                     MKILSGFIQADPGGEIVLDGKSVVMQSPADAIRFGVGMLHQDPLDFPPMKLIDNFLLG
                     MSGKLFPNRKETVQDFNLLAKEYGFNFDPENYVDSLTVGERQQLEIMRLLFLGARVFI
                     LDEPTTGISAAQKIKLFDTLRTFPEQAITTIFVSHKLEDVESLCDRVAVLRAGELVGE
                     FTPPYETKKFVSTMFGKEITLGERQPAKTGDVVLSLKDVSLEDPRVRIDHVNVDIRAG
                     EVIGLAGLEGSGQGMFIRACAGLVRPVGGKVMLKGKNLTGKPYHTFKHEGVNFLPAAR
                     LEEGLVPGLSLTDHFVLAEEPKGVFIDKAYGQKLAEDRIKSFNIRGTPASMIESLSGG
                     NQQRAELALLQTPLSLVLLEHPTRGLDIESVIYIWSKLKDRCKQEGAAIIFISSDLEE
                     VLQYSDRLLVFFSGKVSEPIDAATTSVEQLGQLIGGKGWTKKGDIHA"
     gene            10930..>11680
                     /locus_tag="PLG52_00205"
     CDS             10930..>11680
                     /locus_tag="PLG52_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39887.1"
                     /translation="MLKTKAGGFIFQLIAFLLAMGFVSLILLAVGASPIEAYKNMLTG
                     SVGSMRKFAEVLVSFVPLLLVTAGLLITFSAGLWNIGVEGQIVLGAIGSVWVLRQFVD
                     STVSPAIVMTLAILGGMVAGALWASLAGALKTFGGVNEIFGGLGLNFVSTALTLYLIF
                     GPWKREGVASMSGTEPFPDAFSLPQLPDLRISPYALVIALIAIVIVYFILQGTYFGLK
                     IKAVGKNIRAAYLLGIPTWQYSLYSFLICGAL"
     assembly_gap    11681..11780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11950..12948
                     /locus_tag="PLG52_00210"
     CDS             11950..12948
                     /locus_tag="PLG52_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB39888.1"
                     /translation="MNQETLLVGLAGVLAAAAPIIFAVMGETLSERAGVINLSMNGTI
                     LLTAMSGFAVAYKTDSLILGFLAGMLIGALVALVVAFASITLKQSQVAIGFVLALTCR
                     DLAYFLGNPFMGVTGPRLQATPIPGLSDIPILGPVFFRQEVITYVSFLAIFIAWLWIY
                     RTRPGLMLQGIGEKPAAAYVRGANVNLMRYVYTVLGGALIGLAGPTYSLGVRAGWMGT
                     ISGLDGIGWIALSITIFGGWNPARAAFGSYLFAFLQWLGLVLQPVLTQVPSQVLQVAP
                     FPLMILTLLLVNIGNAEWVERTLAAMPESARKFTAKLLRAMRASPPAALGVPFERD"
     gene            13027..14295
                     /gene="thrC"
                     /locus_tag="PLG52_00215"
     CDS             13027..14295
                     /gene="thrC"
                     /locus_tag="PLG52_00215"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNB39889.1"
                     /translation="MNKFNGYRCSICGSEYLPGQVTYTCPKDGGNLDVVLDVDTIKKK
                     FQPEDLISRTEASLWRYLPLLPVNEPVGDSTPLHASGWTPVFALPRLAEKLHLKHLWL
                     KDESRNPTASFKDRASALVVTRARELKSEIVVTASTGNAGAALAGMAAAVGQKAVIFA
                     PKTAPQAKVAQLLVFGAKVILVDGTYDDAFDLTVKASNEFGWYCRNTGYNPFTLEGKK
                     TAAFEIWEWWIEAHRDWHKKDSTLDNHSPLTIFVSVGDGNIISGIHKGFKDLLALGWI
                     SNMPRIIGVQAEGSAAIANAFHAGTETITPVSATTIADSISVDLPRDGVRAVRAAQQT
                     DGTYIVVKDEDIIKAIAELGKIGVFAEPAGATAYAGLVKATSQGVVGSDDPVLALNTG
                     SGLKDVRAALQAVQSAPIIEPTLEAVKKIL"
     gene            14292..>15023
                     /locus_tag="PLG52_00220"
     CDS             14292..>15023
                     /locus_tag="PLG52_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNB39890.1"
                     /translation="MKKINDQWSLPFRYAMGIIIFILVVAFLIYAREAVTMLVIAGFI
                     AYLISPAVLILKERTGWSRKVAVNVVYFTALIVLVGIPATLTPIFFDEIQLVAQDVLE
                     LSQDFSNFLSEPIQMGGLVIHLEQVGESLAHIQDSILTPLPEEALQLIEATSINILWV
                     LIILVAVHLFMSEWPKMHSWLIHLAPEPYQDDMRELYNRLRNVWMAYLRGQIVLMLVV
                     GVTFTIAWAVIGIPGALVLGMIAGLF"
BASE COUNT         3509 a         3353 c         3948 g         4113 t
ORIGIN      
        1 agccatcata tgatcgtggt gggcaacgat gatgaaagca tggctgttgc cgcgaatgaa
       61 ctggcgaagt gtggcggcgg tcagatcgtg gtgatggatg gcaaggtgat tgggcaggtt
      121 gagttcccaa ttgcaggttt gatgtcgaat gaacgtgccg atgtggtggc aaagaaagcc
      181 gcaacggtgc ttgaaggttt caagatgtgc ggcagtgaat tgaataaccc caatatgcaa
      241 ttgagtttga tggcattggt ggttatccct gaattgagaa tttctgataa gggtctggtg
      301 gatgtgaccc aattcgattt cattcctgtg ttggaagact aagagagaga acaacctatg
      361 gctaattttc cttttcaacg attacaaact gacctcgctg cgttgatcaa taaaacacca
      421 gcagggcagc gcttgccttc tgaacctgag ttggcaaaac aattgggtgt gtcgcgcgcc
      481 accttgcgcg aagcgatgcg ctcttttgaa acacagggct tgatccgccg cagacaaggt
      541 tcgggtactt ttgtggtggg taaagtgcaa gcattggata gcgggcttga agtgctggaa
      601 agcctcgaga cccttgccaa gcgcatcggg ttggaagtct cggtcagtga cttgagcatt
      661 gaagccatcg ctgccgatgc agatttggcg gcttcgttga acgtcactgt gggtgccaag
      721 ctgacccgtg ttcgacgtgt catccgcgcc gaaggacgcc cggttgcgta tctggtggat
      781 accttgcctg aggattttgt gcacgccaaa gatgtccctt cggatttcca cggctctgtg
      841 ctggactttt tgctgaatcg cggtgacctg ttagcttctt ctcgcgccaa tgtgagcgcc
      901 atcggtgcca cagccgatgt ggcaaagccc ctcgaaattc aacgtggcga cgtgctcttg
      961 cacttcaatt ctcagctttt tgattctgaa ggcaaagtgg tcgactactc attgagctac
     1021 ttcatccccg gttacttccg cttccatatc gtccggcggg ttgggaacgg gtaattacat
     1081 atattcagga ttctctacga accctgtgta attctaagga gaaaaatatg accgatacaa
     1141 caaaagaaat tcaaaaaaga gttcaatctt cgcgagaaga tattatcgaa ttcatgcgcg
     1201 acattgttgc aattccatcc atggactctc agattggtcc tgtgggagaa cgcattcaag
     1261 cagagatgaa gaaactgggc ttcgatgaag tccgctttga caagatgggc aataccattg
     1321 ggcgtattgg caacggcaaa cgggtgatgg tttacgactc gcacatcgat acggtgggta
     1381 tcggtgaccc gtcatcctgg caatgggatc cattcaaggg caagatcgaa gatggtattt
     1441 tgtacgctcg tggtgcctgt gatgaaaaag ggtcaacccc cggcatgatc tacggtctcg
     1501 ccattgcacg tgacctcggt ctgctcgatg gctggacggt ctattacttt ggaaatatgg
     1561 aagaatggtg cgacggcatc gctcctaata ctttcgccga agtagatccg ggcatcaaac
     1621 ccgattttgt cgtcatcggt gaaccgacca agatgctggt ctatcgcgga cacaaaggtc
     1681 gtttagagat gaaggtgacc gctaaaggca agtctgcgca tgcggcaagc aatcaccttg
     1741 gtgataatgc catttataaa cttctgcctg tgatcgccgg cattcgcgat ttagaaccga
     1801 agttgggtga ccatgaattt ctcggtcacg gcaagatcac ggtcagcgat atgcacgtca
     1861 aaacgccttc catcaacgct gtcccagatg aggcggtgat cttcatcgac cgccgcatga
     1921 cctttggtga gactaaagaa gcggtcaaga aacaagttga agatttgatc cctgctgaat
     1981 ttaaagacac cgtcaagctt gaagaattgt tctacgatga gccttcttat acgggtttcg
     2041 ttttccccgt tgataaatat ttcccggcat gggcgtatga agaaaatcat ccgctggtgc
     2101 aagctgggca agaagctcgc aagttcatcg gtttgcccga agcaaaaccc agcaaatgga
     2161 acttctctac gaacggtatt tattgggctg gtaaagcggg cattccctcg attggctttg
     2221 gccctggtga tgaagagacg gctcataccg ttaatgattc tgtgtcgctg gaagatatgg
     2281 tcaaggcgac agagttttat gccatcctcc caagcttgat caaataaaca atttacggct
     2341 atgaaaattt tagaggtacg acgtcattcg attcgccatg ctggcggcga ccatttgagt
     2401 caagccggcg ttgctctggc tcgtaatgtt ggtcagaaca tcggtccctt cgattttgtg
     2461 tcaacatcca cattgccgcg tgcatttgat accgccatcg caatgggatt tgcggtcaat
     2521 gagcaaaatc cgctcatgaa tacgtacggt ggcgccgtag agcgagaagc tccctggccc
     2581 atgccgttct tccattattc agagatcgtc aagcaggcag gggcggctgc gaagtacgca
     2641 catgacttga tggattacta cctgggtatt ctcaatcaca ttccgcaagg cggttcggcg
     2701 ttggtggtga accacggcgg tgtggtggaa ctgggtgttg tggcatgcct gcccgatgca
     2761 gatttttctg catggggtga tgctgtggag tattgcgaag gtgcccgtct cttttgggat
     2821 ggcggcaaat ttgtgaacgg cgaagtgttg cggcttatgt ataacaagga gagtttgtga
     2881 gaaaagcttt accggttctt tttatcttaa ccatgttgct tttagcatgc ggcagcaaca
     2941 cagccaaacc caaagtggct ctgatgttgg cgcgcggcgg cttgggcgac aaagccttta
     3001 acgatagtgc gaatgaaggt ttgcaaaaag cggctcaaga attaaatgca gaggtcacaa
     3061 cctttgacta tgccgaagat cgtgtagagg caaacatccg ccaagcggca gaagatgggt
     3121 atagcctcat cattggattg ggttctgaaa attctcaagc cattgctacg gttgccaaag
     3181 aatttcctga aacgaagttc gccatcatcg atgcaactgc agaaggcgac aacgtcacct
     3241 cggtcacatt cagtgaactc gaaggcgatt ttcttgcagg agctttatct gctttgatga
     3301 gtgagaatgg caaggttggt tatctgggcg gggcagatgt gcttgtgatc cgccgtattc
     3361 agtatggctt tgagcaaggc gtgaaataca tcaaccccga tgctgaaatt gttgttcagt
     3421 atatcggcgg caaagatgat ttcagcggct tcgccaaacc tgatgaagct caaacgattg
     3481 ccgatcaaat gtatagcgat ggtgtcgata ttatttatgc ggcggcgggt gggtccactc
     3541 tcggggcgat caaggcggct gaaagcgcag gcaagcccat cgtcacgacc ggaagcgacc
     3601 agcgttattt agctcccaat gtggtggcga ccagtcgcac caagaacatg aacgaggctg
     3661 tctttatgct gatccaaaaa tttgtggatg acagcctgcc aagcggaacg atccaactgg
     3721 attacaagtc tggtggcatt ggtcttgcgc ctcttgccga ctttgtgcct gcttctgttt
     3781 ctgaggcttt tctctttgtg aaatcggata ttgaagacgg tgccatcaag gttgagccgt
     3841 ttacagaata ataaaggttg gtttaacgac caactagaaa agataaggag ataaattatg
     3901 caaacgaatt ttcgtggtcg tgattttatc ggcgacctcg acttctcgaa agaagaagtc
     3961 gaaaccgttt tagatgtggc gtgggatcta aaacgcaaac gcgccttggg tgaatctcat
     4021 gccttgctgc gcgacaagac tctggctatg ttgttcttct tcacgtccac ccgcacccgc
     4081 gggtcgttcg aagcgggcat ggcgcagttg ggtggtcatg ctgcctttat tgacagcgaa
     4141 accacacaga tctctcatgg cgacactgcc aaggaaatcg gtgagatctt cggacgctat
     4201 tttgacggca ttgccatccg ccaatgtgac tggaacttcg gcaatcaata catcaatgat
     4261 gttgccaaag ccagccgtgt gccgatcctt aatatgcaat gcgatgtcta tcatcccttc
     4321 cagtgccttg cggacctgat gaccattcag gaaaagaagg gacgtgacct caagaaaaag
     4381 aaggtggtgg tttcttgggc gtatgcggct tcctattcca agcccatctc tgtgccacaa
     4441 tctctaattc ttcagatgac ccgctttggc tgcgatgtgg tgttggcgca tccaccagag
     4501 ttcaagctta tgcctgagat catgcaacag gcgaaagaca atgccaagaa atatggcgtt
     4561 ggcttcgatg tgaccgacaa catggatgaa gctttcaagg atgccgatgt ggtctacccc
     4621 aagagttggg gtcctatgct gaccaccacg ggtaaggaag aaggcaaagc actgattgaa
     4681 aagtacaagg actggatcac ggacgaacgc cgcatgaagc tgaccaagga tgactccatc
     4741 tatatgcact gtctgcccgc tgaccgcggc ttggaagtga ccgatggcgt gattgatggt
     4801 ccgcactcag tggtgtacga cgaagccgaa aatcgcctgc atgctcagaa ggcggtcatg
     4861 gctttgacga tgtcttaagt taatttgcag gggcacactc cagtgtgccc ctgctagtct
     4921 tttgctcagg agactttata gatgacaaat cttgctgtga ttgcagtggg cggtaactcg
     4981 ctcattattg atgataagaa agtaacagta gaaagccaat atcaggcggc aaagcaaacc
     5041 tgtgtgcaca ttgccgatat gatcgaacaa ggctgggatg tggcgattgg gcatggcaat
     5101 ggtccacagg ttggctacat tttgcgccgc tctgaaattg ctgccaaaac ggctggcatg
     5161 catgaagtcc ctttggatgt gtgcggtgct gatagccagg gcgcaatcgg gtacgccatt
     5221 caacaaaatt tgcagaacat tctctaccaa cgtggcatca agaaaaaggt tgttaccgtc
     5281 atcactcaaa ccttggtaga taaaacagac ccggcatttt caaagccagc caaacccatt
     5341 ggcagcttta tggataaaga agaagcggaa cgccgtcagc aggaacaagg ctggtctgtg
     5401 gtggaagatg caggtcgtgg ttggaggcgg gtcgtcgcct cgcctttgcc aaaagaaatc
     5461 gtggaattgg aaaccgttca aaccctgttg gaaaaaggaa tcatcaccat tacagtaggc
     5521 ggcggcggca ttccggtcgt tgaccctggc gatggaaact atgaaggcgt ggcggctgtg
     5581 gttgacaagg attacgcctc ttctttgtta gcccgctcta ttggtgcgaa tctatttgtc
     5641 atttcaacgg cggtggaaaa agttgccatc aactttggta aacccgagca aaaatggctg
     5701 gaccagatca cactagccga agccaaagcg tacttggctg agggaatcca ttttgccaaa
     5761 ggctccatgg cgccaaagat ccaagcgatt atttggtatc tcgaagccat gccgaacggc
     5821 aaagctctaa tcaccaaccc cgaaaatata gggcgcgcgc tcaaaggcga aaccggtact
     5881 tggattgttc catagacgat gtaattccgc tgccaatttc ggcagcggat tcgtttacaa
     5941 ttgttccaat gaaaaaattg actgaccgtt tgaacaacat ccaatttaca ccgcgcagta
     6001 cggtttggtt tttgctgtta attgccgcgc taacgtatac tcttttcttt tggcagcgcg
     6061 gtttttattg ggatgaattc ccatgggcat ggatctacta tcggttgggt cctgctatgc
     6121 tcacgaaaac atttaccacc agccgtccgt tttgggggat gatctatcaa ctgaccatgc
     6181 ctgtcatcgg tcccaacccg tggatatggc aattacttgc catcgtctta cgatgggtga
     6241 cagccgttct gctttggaaa attttacgcg cactttatcc tgaacatccc aaacctgctt
     6301 tgtgggcaag tttgttcttc cttgtattcc ccggcatggg gcagcagttc atcgcattca
     6361 tgtacagcca tttttatatt gtgctgtctg ctttgttgct ttccatgtat cttgcaattc
     6421 gcgccattca agatgaaaaa agacgctggc tttatttttc aatatcgtat gttctggctt
     6481 tcgttaattt cattacgatg gagtattttt acttcattga atttggtctg ctgattgttt
     6541 tttaccagct cttgccgggc acgttcaaag aacgtgcgaa aaagacgctt ctttattttc
     6601 tcccttatct tgccatcttt attggcatca cggtttggcg cactttcttt ttcgaatttc
     6661 aaaatgcgag ttatcaattt gttttgctcg ataaactaaa agcggatgtt ttctctggac
     6721 tgctttattt cttaaatcaa attctggtca gtttttggcg caccgtgttt gaggcgtgga
     6781 ttttgccttt cctcaccatt ggcaccacag gctttggtcc gctcacactg tctttgatgc
     6841 tggttctgtt attctccagc attctcctca ctggagtgta tctcttcgcg ttcaaagcag
     6901 agtctgcaaa cggtttggat tttgccaaac gagcaggctt ggtcggcttg gcgttttggg
     6961 gcttatctgg cggctccgtg tggatgattg gcaatttgcc gcagttcaat ttttcgctag
     7021 accgtttcat gttgccgttt atgctaggct ctagcatttt gttggcaagc ttgatcgcgt
     7081 tgatcaaaag tccgcgctgg cagatgattt tagtggcgct gttaattggc tttgcaggta
     7141 gtagacattt tcggttggaa gagatctatc gtagtgattg gttgacccaa caggaattgt
     7201 ttacacaaat gacggaacga atccctgggt tgaaaaaagg aactatctta atttctaatg
     7261 acattcctgt gacatattac tctgataatt cactcacagg accattaaac tggatttaca
     7321 gtccgcaagg cgaaatgaat gtgattttgt attttgcatc cattcgtgtg gggaagacat
     7381 tgccaagcgt agagccaggt caacagcacc agttgtatta tattggaccg accttttatg
     7441 gcaataccga taatattttg gtggtgaatt ttgagccacc tggttgtttc cgcgtggtcg
     7501 acccggaggt ggaagtggat aatcgtttgc tttcccctgc ggtgcgtgat gtggcgcagt
     7561 attcgaatca gagtgtgatt ctgttcgaga aacagaatca gttaccgggt cagctttatg
     7621 gaggcgcgcc ggaggcggat tggtgtcatt attttgagca ggctgagctg gcgcgtcaac
     7681 agggcgattg ggaaactgtt acccatttgg gcgatgcggc gttttccttg agcgattatc
     7741 ctaacgaccc gctggaaagg tttgtataca tagaaggata cgctcatcaa tcgaactgga
     7801 aaaaggcggt agaattatca aaggaaacct acaagatttc taaaacatat gtaggtcctt
     7861 tgttatgccg tttatggggg cgtatagagc gggaaacgga agcttcttcg gagcaggttg
     7921 cggctttgga gttggttcga agtgagtttg ggtgcttacc ttaaaaatac gttgtacctt
     7981 tcttcgcaca tagaaagttt agtaaaattg tttagacaac tgaaaggagg ttcgaaagaa
     8041 gatttcgttc ttggtcagtc gtcaaacaga aaatatttca aaaaggagaa gaagtatgcg
     8101 taatttattt gcacgcctcg ccgttttggc gttggcacta atgctcgttt tgtctgcctg
     8161 tggtggacag cccgcagcga ccgaggaggc tcctgccgcc acggatgccg ctcctgctgc
     8221 aacagatgca cccgctgcta ctgatgctgc tcccgctgct gagcccctta ccttcggtat
     8281 gttgctcgtt ggtccgtaca acgacaatgg ctggagccag gctcactatg aagctggtaa
     8341 gtatgtcgaa gaacagctcg gcgccaagat gctctacctc gacaaagtta accctgcaga
     8401 tcgccccggt accacccccg accagctcgc tgaagaattg gtggcacagg gcgctaccct
     8461 cattatcttc aactctgatg atatgaagga tgcctccacc accttcgcca agaatcaccc
     8521 tgatgtgtat gtcatcatgg cttctggtga tcaggtttgg tctgacggca aggcgtttga
     8581 agccatgccc aacctcacca acatcatggg acgcatggaa tacggcaaga tgatggctgg
     8641 ttgtgccgct gctctcgctt ctgagaccgg taagatcggt tacctcggac cgctaatcaa
     8701 cgatgaaacc cgccgcctcg ctgcctctgc ctacctcggt gcgaaatact gctacgagaa
     8761 ggctggtaat gatcctgctt ccttggaatt caaggtgact tggattggct tctggttcaa
     8821 catccccggc gtgacccttg accctgttca ggtttcgaac gatttctaca ccaccgatta
     8881 cgatgtggtg atctctggta tcgataaccc tgtcaacctt ggtgaagctg caaaactcaa
     8941 ggctgaaggc aagaatgtct ctggtgtggc atatgactat gtcgccgctt gtgaagctgc
     9001 tcctgatgtt tgtatcggtg ttccgtactt caactggggt cctgaatatg tgaaggcgat
     9061 ctcctccgct caggatggtt cctggcagtc tcagttcatt tgggctggac ccgattgggc
     9121 tgacatcaac aacccagaca cctccacggt tggtttcgtg aagggacctg ccttggctga
     9181 cgatgatgcc gctaccctcg atgcatttat tgccgaactc gctggtggac tgaacctttg
     9241 gtctggtccg atcaacctgc aagatggaag cgcctacctc gccgacggtg aagttgctac
     9301 agaccagcag atctggtacc tgccgcaatt gctcgaaggt atggatggct tgagcgcagc
     9361 cgaataatgt agttcccttt gtaggggcgc ggacaccgcg cccctacatt ttcatttcac
     9421 cctcatggag gacccgcacc accatgaaag tcgaactcaa gaacattcac aagcacttcg
     9481 gcaagaccta tgccaataag ggaattaatc ttaatattcc ttctggctcg atacagggta
     9541 ttttggggga gaacggcgca gggaaaagca ccctgatgaa gatcttatcc gggttcattc
     9601 aagcggaccc aggtggtgag atcgttttag acggaaaatc tgtagtgatg caatctccag
     9661 cagatgccat ccgatttggg gtggggatgt tgcatcagga cccgctcgat tttcctccca
     9721 tgaaattgat tgataacttt ttgcttggca tgtcgggaaa acttttcccg aatagaaaag
     9781 aaaccgtaca ggactttaac ctgctggcaa aagaatatgg ttttaatttt gaccccgaaa
     9841 attacgtaga ttctctaacg gttggtgaac gccaacagtt ggagatcatg cgtttgttat
     9901 tccttggtgc gcgtgtgttc attttggatg agccgacgac gggaattagt gctgcgcaaa
     9961 agataaaatt gtttgatacg cttcgaacat ttccagaaca agccattaca acaatttttg
    10021 tgtcacataa attggaagat gttgaaagct tgtgcgaccg tgtcgccgtg ttgcgagcag
    10081 gagaactggt tggtgaattt acaccgcctt atgaaaccaa gaaatttgtt tcaaccatgt
    10141 ttggcaaaga gattacttta ggtgaacgtc aacctgccaa aacgggtgat gttgtgcttt
    10201 cgttgaaaga tgtctctttg gaagaccccc gtgttcgcat cgatcatgtg aatgtcgata
    10261 ttcgtgctgg agaagtgatt ggtcttgctg gtttggaagg ttctgggcaa ggcatgttta
    10321 ttcgtgcatg tgctgggctc gtccgcccgg tgggtggtaa ggttatgctt aaaggtaaga
    10381 atttgacggg gaagccgtat cacactttca agcatgaagg tgtcaacttc cttcccgccg
    10441 caagattgga agaaggctta gtgcccggct tgagcctgac cgaccacttc gtccttgcgg
    10501 aagagccgaa gggcgtgttc attgataagg catatggtca aaaactggcg gaagatcgca
    10561 tcaaatcctt caacattcgc ggtacgcctg ccagtatgat tgagtcgctt tcgggtggaa
    10621 atcaacagcg ggcagaattg gcgcttttac agacgccact atcgctggtc ttattggagc
    10681 acccaactcg tggcttggat attgagtcgg tgatctatat ttggagcaaa ctcaaagacc
    10741 gctgcaaaca agagggcgca gccatcatct ttatttcgtc tgacttggaa gaagttttac
    10801 agtacagtga ccgtttgcta gtgttcttta gcggtaaggt ttcagaaccg atcgatgctg
    10861 cgacaaccag tgtggaacaa ctaggtcaat tgatcggcgg taagggttgg acgaaaaaag
    10921 gagacatcca tgcttaagac caaagcgggt gggtttattt ttcaattaat cgcgttcctg
    10981 cttgctatgg gatttgtctc gttaattctg ttggcagtag gcgcttctcc tatcgaagcg
    11041 tataaaaata tgctgaccgg ctcggttggc tctatgcgta aattcgccga agtgttggtg
    11101 tcatttgtgc cattgcttct ggtaacggct ggtttgctta ttaccttctc tgccgggctt
    11161 tggaatatcg gtgtggaagg tcagattgtt ttaggtgcca ttggctcggt gtgggtctta
    11221 cgtcaattcg tagatagtac ggtttctcct gctattgtga tgactctggc gattttaggc
    11281 ggaatggttg ctggtgcctt gtgggcatcg ttggcgggcg ctttgaaaac ctttggcggt
    11341 gtgaatgaaa tttttggtgg cttggggctg aactttgtct ccacggcatt gaccttgtac
    11401 ctaattttcg gtccatggaa gcgtgaaggg gtggcgtcca tgagcggaac cgaaccgttc
    11461 cctgatgcat tctctttgcc gcaattgcca gatttacgca ttagcccgta tgctttagtg
    11521 attgcattga tcgcaattgt tattgtgtat ttcatcttac aaggtactta ctttggtttg
    11581 aagatcaaag ccgtaggtaa gaacatccgc gctgcgtatt tacttggtat tcctacctgg
    11641 caatattctt tatactcttt cctcatctgt ggagccttgg nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn ttgccagtcg ctcttttctt tgcggcgttg aacattggta
    11821 gcattcaatt gccaattgtg atgcaattgg attcgtcact ttcgggtgtg ctgcaaggtg
    11881 ttttggtgct cttcgttttg ctcactgaag gggctcgcca aaaatttggg aagaagggat
    11941 aaggcatcca tgaatcaaga gactcttctt gttggcttgg cgggcgtact tgctgcagcg
    12001 gctcccatta tttttgcggt gatgggcgag accctttctg aacgagctgg cgtcatcaat
    12061 ttatccatga acggaactat tctgttgact gctatgagcg ggtttgcagt cgcttataaa
    12121 acagatagct tgattcttgg ttttcttgct ggcatgttga tcggtgcgct tgttgcttta
    12181 gtggttgcat ttgccagcat taccctcaaa caatctcaag tagcaattgg ctttgttttg
    12241 gcgctcacat gccgcgactt ggcatacttt ctcggcaacc catttatggg tgttacaggt
    12301 ccgcgtttgc aagcgactcc cattcctggg ctgagcgata ttccgattct cggtcctgta
    12361 tttttccggc aggaagtgat tacctatgtc agcttcttgg caatcttcat tgcctggtta
    12421 tggatttatc gcacgcgccc cggtctaatg ctacaaggca ttggcgaaaa accagcagcc
    12481 gcctatgtgc gtggcgcgaa tgtgaatcta atgcgctatg tttatacagt tctgggcggt
    12541 gctttgatcg gtttagcagg acccacgtat tcgttgggcg tccgcgctgg atggatggga
    12601 accatttccg gcttggacgg aatcgggtgg atcgcccttt ccatcacgat ctttggtggt
    12661 tggaatccgg ctcgcgctgc gtttggttca tacctcttcg ctttcttgca atggcttggg
    12721 ctggttttgc aaccggtgct tacccaagtc ccttcgcaag tcttgcaggt tgcgcctttc
    12781 ccgttgatga tcctcaccct cttgctcgtc aatataggca atgcggaatg ggttgagcgc
    12841 acattggcag ccatgccaga atctgcgcga aagtttacgg cgaagttatt acgagccatg
    12901 cgtgcctcgc cgccagctgc tttgggcgtc ccattcgagc gtgattagtt gcaacataaa
    12961 aggactctcg tatgagggtc ctttttgatt tttatatcgg gtaaaattaa gccctagagg
    13021 aaatgtatga ataaattcaa cgggtatcgt tgttcaattt gtgggagtga gtatcttcca
    13081 gggcaggtca catatacgtg tccaaaagat ggcgggaact tggatgtggt cttagacgtt
    13141 gataccatca agaagaaatt tcagcctgaa gatctcatct ctcgaacaga ggcttcgctg
    13201 tggaggtatt taccgctgtt gccggtcaat gaacccgtag gcgattccac gcctcttcac
    13261 gcctccggct ggaccccggt ctttgccctg ccacgattgg cagaaaaact tcacctgaag
    13321 catctctggc tcaaagatga gtcgcgcaac cctactgcct cgttcaaaga tagagctagc
    13381 gccttggtcg taacgcgtgc ccgcgagctc aaatcggaga tcgtagtcac tgcttcaacg
    13441 ggcaacgccg gagcggcatt agcaggcatg gctgccgccg ttggacaaaa agcagttatt
    13501 tttgcgccca aaaccgctcc acaagcgaag gttgcgcaat tgctggtctt tggtgcaaaa
    13561 gtcattctgg tagatggaac ctatgatgac gcctttgacc tgaccgttaa agcctcaaat
    13621 gaatttggtt ggtactgtcg taatacaggc tataacccgt tcacactgga aggtaagaag
    13681 accgccgctt ttgagatctg ggaatggtgg attgaagccc atcgtgattg gcacaagaaa
    13741 gatagtacct tggataatca ttctccgtta actatctttg tctctgtagg ggacgggaat
    13801 attatctctg gcatccacaa aggcttcaaa gatttattgg cactcggctg gatttcaaat
    13861 atgcctcgca ttattggtgt gcaagccgaa gggtctgcag cgattgctaa tgccttccat
    13921 gcagggacag aaaccatcac acccgtatct gccacaacca ttgcagatag catctcggtc
    13981 gatttgccgc gagacggtgt ccgtgcagtg cgggcagctc agcagaccga tggcacgtat
    14041 attgttgtca aagatgagga tattatcaaa gccattgcag agctgggaaa gatcggcgtc
    14101 tttgccgaac ctgctggggc gacggcatat gccggtttgg tcaaagccac aagccagggc
    14161 gtggttggaa gcgatgatcc tgttctcgcc ctgaatacag gcagtggact caaagatgtg
    14221 cgagcggcat tgcaggcagt acagtccgcg ccgattattg aaccaacttt agaagcggtg
    14281 aaaaagattt tatgaaaaaa attaacgatc agtggagcct cccctttcga tatgcgatgg
    14341 ggattatcat ttttatcctt gtcgtagcat ttttgattta tgcgcgcgaa gcggtgacca
    14401 tgttggtgat cgctggcttt attgcatatt tgattagtcc ggcagttttg atattaaaag
    14461 aaagaacggg ttggtctcgt aaagtagcgg tcaatgtggt gtatttcacg gcattgatcg
    14521 tgttggtggg aatcccagcc acgttgaccc cgatcttctt tgatgaaatt caactggtag
    14581 ctcaagatgt tttagagctc tcgcaagact ttagtaattt tctttctgaa cctattcaaa
    14641 tgggtggctt ggtaattcat ttggagcaag tgggcgagag cttggcgcac attcaagata
    14701 gtattttgac gccgttgcct gaagaggcgc ttcagttaat cgaagcgact tctatcaata
    14761 tattgtgggt gttgattatt ttggtggcag ttcatttatt catgtctgaa tggccgaaaa
    14821 tgcattcttg gctgattcat ttggcacctg agccttatca ggatgatatg cgtgaactct
    14881 ataaccgttt gcgcaatgtg tggatggcgt acctgcgtgg tcagatcgtg ttgatgttag
    14941 tggtaggtgt gacctttacg attgcttggg cagtgatcgg tattcctggg gctttggtgt
    15001 tgggtatgat cgcaggcttg ttc
//
LOCUS       DAOQNJ010000004        14568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_1677_length_14568_cov_9.948460, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000004 DAOQNJ010000000
VERSION     DAOQNJ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14568
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1677_length_14568_cov_9.948460"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..639
                     /locus_tag="PLG52_00225"
     CDS             247..639
                     /locus_tag="PLG52_00225"
                     /inference="COORDINATES: protein motif:HMM:NF036989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39891.1"
                     /translation="MTETEKITINLGAVDLGKIDLLVEQGHYSNRTDFIRSAIRSQLD
                     KHVLEVQQAVTRYSFGVGAFFHSRADFEKYKAKGDKIKIDVIGIYHVPDDVSVALALE
                     VVESIKVYGIFQARSELKDALQKAGKIR"
     gene            636..1931
                     /locus_tag="PLG52_00230"
     CDS             636..1931
                     /locus_tag="PLG52_00230"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB39892.1"
                     /translation="MTSEQQVPENWAIRFFTIFAGQAFSLFGSALVQFALIWYLTQKT
                     GSATVLATASLFGMLPQVLLGPIAGTIVDRGNRRLIMILSDSLIAISTLLLAYLFWSG
                     AAEIWHIYVALTIRSAGGAFHYPAMASSTSLMVPDKHLSRVAGANQTLQGLISIVAPP
                     VGALLVAAMSTQNILMIDVITAIMGVTPLLFFTIPQPPRKAEQTATQTSFLKDLGAGF
                     KYMASWPGLLMLGLLATVLNFLLAPLTALMPLLVTKYFKLGALELGSLDSFFGIGMIA
                     GGITLGVWGGFKKRIVTSLSGIILTSFSILAIAGAPSNAFWMVVAAMAFMGFMMPMVN
                     GPIHALFQSVVEPDMQGRVMSLISSVAQAMMPIGFILAGPITDAISFQAWFWVAGILN
                     LLIGVSGFFIPAVINIEENRKAYHPAESNESTPITEFSN"
     assembly_gap    2051..2150
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2151..3806
                     /locus_tag="PLG52_00235"
     CDS             <2151..3806
                     /locus_tag="PLG52_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39893.1"
                     /translation="FALGLAGLWASGNTQSVILNGLLPLTDAAGYYTDALRLQYGSNV
                     SNFTAMRPLYAGLLSFFLWISNNNLMIAVSIFTAITGFSCYLACKEIQKTHGAEVAVF
                     FLILTFLYYRHHSGTTMSESLGVPVSLLGVALLWKGIYSKNEGTTLFGIGMIALALNI
                     RPGAMFVLPGLLLWGGWIFRKEKKFSFKFFISGLTVVLSVFYINSRMIDFVAGPSGVA
                     FENFSWAFYGLSSGGKSWTYIFEAHPELRLLQDSEVTPTIYKLAFKQILNNPSLMIKG
                     SFFYWRMFFSDSWYNAYAFVAGENYWISEIARWGMYILGALGIYKLLKNYQDPYTSLA
                     GITAVGVLTSVPFVPPTDAYRVRLYAATIPFFILLPGMGVSLLLDAVPQRLLSPKFTF
                     LNKNSTSAIFSIVLILTLLLSPIYIKTAGTSPTFSQTSCPASLDSFLIRYDDGSSFTV
                     YRESQVFIDQMPDFHMSTFRRNAHSLPDMNLITTLENIEPPKTITYSIDLLTNNQAII
                     IAEPELLPVSGVYLLLCGKFIYEPNFTPYSFFYVEQVFSVEGH"
     gene            3855..3944
                     /locus_tag="PLG52_00240"
     tRNA            3855..3944
                     /locus_tag="PLG52_00240"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3891..3893,aa:Ser,seq:gga)
     gene            complement(4040..4699)
                     /locus_tag="PLG52_00245"
     CDS             complement(4040..4699)
                     /locus_tag="PLG52_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39894.1"
                     /translation="MKDDVRKYIYGAIAVFLVGVLAWVGIVFVSACGVSLSCNRGNTL
                     PDTTPVPTLIPATLPPMTVESNIVAPSDKCYVAGSDLIGAWVDAGSPEADVFEFTDAN
                     GATCEATFEDVQPLFTKANLWYSGSLSCISCHSVDLAVSPAQLDMSSYEGILAGSRRT
                     DDAPKGTDILGGGKWKSSLLYQFIVEAHADVPGHDAALSSDLMVYAGTPLPDVKATPT
                     P"
     gene            complement(4712..5263)
                     /locus_tag="PLG52_00250"
     CDS             complement(4712..5263)
                     /locus_tag="PLG52_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB39895.1"
                     /translation="MRLFKQLVAVFAVLAVLLAILMTFSYDIIKIQWVTFMAIQPSFD
                     DQEKPLAVPAQSIPVDGAAYLPGMGEPTNPVPADETSIARGKEIYQINCMMCHGETGQ
                     GNGTVAAFLVKKKPADLTSEPVQAKSDGSWFLTISNGIWNPNNTLFPERQFSGQMPPL
                     AENLSIRERWDVINYLRTLKASQ"
     gene            complement(5260..5802)
                     /locus_tag="PLG52_00255"
     CDS             complement(5260..5802)
                     /locus_tag="PLG52_00255"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNB39896.1"
                     /translation="MTETKNTVVDILAVFSDLAPAADAIEQLRALGVSDAHMNVISGV
                     PVTEAMLGRPSQWTNVPRIALGGAILGFGAGAFLAFVAPYWYPYPIQVSTQAFVPGPP
                     TVVVLFELTMLGMLLSTFLGVFLDSYFPNYRPMKYVPEISDGKIAIYVECPDCDEKKI
                     TDLLKKLGAESVKPAEAQHL"
     gene            complement(5812..7239)
                     /gene="nrfD"
                     /locus_tag="PLG52_00260"
     CDS             complement(5812..7239)
                     /gene="nrfD"
                     /locus_tag="PLG52_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNB39897.1"
                     /translation="MSDNKHHAAHSHEEHLREKHLMLDPLPRGRMNEMVMESMLEKPT
                     WKFWVIFGFLAFVVVYFLFYKWAILITKGQGEAGVMRPDYWGIFLVNTVFWIGISHAG
                     TFISAILRVFRAEFRRPFTRAAELMTTFGLVQAGFSIFMHMGRVWLFYWLMPYPNERM
                     LWPNLHSPLSWDLLAITTYLLSSTMYLFLPLIPDVAMARDRSTGWRKNLYKILALGFR
                     GTEGEWTHLRNAMNIFAFAIIPVMFSVHTIVSWDFAAATRPGWSSTIFGPYFIVGALH
                     SGMGAAAVVLAVIRSTMKHMDYFVRGEHFDAIGKLMLIISMAWAYFFFNDYMVQWYGG
                     DKWTQQLLHFHEAGPLGWMWFGMLFCNVVVPWAILWNSKWRSTPWAITVVGLIINVGM
                     WFERYIIIPISVTINRMPFTWRMYTPGIEVPMGIGTVALFILLYMAASKLIPLIPVWE
                     VQEGQMAHELKKFGRETVVSVSELE"
     gene            complement(7242..7967)
                     /locus_tag="PLG52_00265"
     CDS             complement(7242..7967)
                     /locus_tag="PLG52_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB39898.1"
                     /translation="MIRPEEIKMSAEETGKWGMVINQDICTGCQACVAACAMENNVTF
                     VGEEDAGYGRSMHWIRIERFWEGEYPEVKMTPQQPMLCQQCGHATCEPVCPAFATVHS
                     QAEQLNLQVYNRCVGTRYCANNCPYQVRAFNWRDYQRPEPLPNQFNPDVTVRRMGVME
                     KCTFCIQRIHKAQDKAKAEGREVMDGEFTTACAQACPANAIVFGRVDQPESMVSQLAH
                     KGHGVKHLEELGTLPNVTYFKGG"
     gene            complement(7970..>9391)
                     /locus_tag="PLG52_00270"
     CDS             complement(7970..>9391)
                     /locus_tag="PLG52_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNB39899.1"
                     /translation="TGAKADHWVPVRPGTEGLVALAIGKLVSELRGGATPRVFSGVKP
                     AEVAEAAGVELAALEHLAEMFANSNALAIPGGPALGQSNGLEIAEAVLALNALSDNFG
                     KPGGVYISALAPNQTEYSRPASAEEMAAFIEQLKSGAIKALFVHGVNPVFELPASLGF
                     KEALANVELISFATFPDETAAEAKYVLPDHHGLESWGYQRVATGVSLPVLSGAQPVVS
                     PFYDTRATADVLIAAAQLAGGKFAEALPYKDEVEFIQTKISSLLSDANGSFTATDPVT
                     FMAYFQQYGGWYKNADSLVAPDGSAVLDSNIKAAEPEFAGEGEFFFVPFVSPTLAEAG
                     ANKPWLQELPDPTTTVMWNTWIEMNPETAHELGIENDDVVRVSSEAGEVEAYVYLYPA
                     IRPDTIAMPFGQGHTAYGRYAEKRGVNPADVLGAHFNGAGDLAFAGMKVKVEKVSSAV
                     RGVGLSRMESRMGVYGEALGEEH"
     assembly_gap    9392..9491
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9492..9991)
                     /locus_tag="PLG52_00275"
     CDS             complement(<9492..9991)
                     /locus_tag="PLG52_00275"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNB39900.1"
                     /translation="MSENFSRRDFLKLAGLGAATTAILTGCGPASRYVKREPYQQMPE
                     YNSVGQSTYYATTCRECAAGCGLVVRTYQGRALKTEGNANHPLNLGKTCARGQATLHG
                     LYNPDRVQGPSTGDWDSAIQTVADAFKTYKPSEIAFLMGMAPDHLFDLVSDLAKETGM
                     NAPVRYG"
     gene            complement(10013..10570)
                     /locus_tag="PLG52_00280"
     CDS             complement(10013..10570)
                     /locus_tag="PLG52_00280"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNB39901.1"
                     /translation="MIKKFFDWLKQPLGLIAVGIVFLAVFSAAAYGLWTTQQAPAQPV
                     PGFPHKTHVAFGIQCLYCHPGAAKGPAAGIPSQAKCWACHNQIVKTRDSALLAPLKDA
                     VLSGTSLEWVPVAIVPDFVQFNHRAHIAAGKNCEECHGDMTQVTIAQNPQVMNMGWCL
                     NCHIQNAGEDQAKLIKLTDCGTCHY"
     gene            10956..11204
                     /locus_tag="PLG52_00285"
     CDS             10956..11204
                     /locus_tag="PLG52_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39902.1"
                     /translation="MKTKNIFNACIFLIAILTLTLVITNFNMPVAAAKDLPVTVLQQA
                     TPTPPVDSSEIGSTSGILVMGVVIVLIIAVPLILQKRK"
     gene            11281..11988
                     /locus_tag="PLG52_00290"
     CDS             11281..11988
                     /locus_tag="PLG52_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB39903.1"
                     /translation="MPEKILIVEDEIALQETLAYNLKKEGYTVETVGDGRSALEAARK
                     LKPDLIVLDIMLPEMDGFEVARTLRKEMTTGILMLTARDDEIDRVVGLEVGADDYLTK
                     PFSMRELIARVKAQLRRARLLREEMGKSVEETPHETLTFDNLIINLTRREVTLNGTPV
                     QLKPKEYELLLFLAEHRRQMLSREFILERVWGWDYIGDSRTVDVHIRWLRQKIEESSS
                     EPKRIVTVRGGGYRFEG"
     gene            12038..13276
                     /locus_tag="PLG52_00295"
     CDS             12038..13276
                     /locus_tag="PLG52_00295"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB39904.1"
                     /translation="MNQFAYATAIVFLIIAAWFAWKYYSLRKNVNQLAKSLHDPKIPL
                     PHLREIEELLSMLESRKATFSAELFSLQTENERLATVLEQLTDGVLIADADGRVQFAN
                     PAASKLFGTHNPTQQSVAQVVRSHQLIEAWKRCQQTRQLQIEAVEIATRKQYLQIIVI
                     PDRHEGGSLLLAQDLTQVRKLETVRRDFISNVSHELRTPLASLKALTETLQGGALADP
                     EAGPRFLDRIHTEVDALTQMTQELLDLSRIESGQVQLNFEMISPRKLIYSAADRMKAQ
                     VERAHLKLDVKCEDGLQNIKADKNRLEQVLVNLIHNAVKFNKPGGSITLSAEPTSGGV
                     RCAVRDTGVGVPMESLNRIFERFYRVDSSRTGSGTGLGLSISKHIIEAHGGKIWAESE
                     EGRGSTFFFIVPLDPSKAKE"
     gene            complement(13239..>14568)
                     /locus_tag="PLG52_00300"
     CDS             complement(13239..>14568)
                     /locus_tag="PLG52_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB39905.1"
                     /translation="RPVIDQTGTSDKTYLFFTVAYLSVDTILFMALWILVTHRSVYLS
                     ISTKIQLGLGILLLILSDTIFLVQDIQGLYFETVRFTDLTYNLSSAFIGAAALYQWST
                     WVELHKVQITKYWVMNLHRLFSDVLLFLSFCLFVYGHQSNQFISFEAVSFWVGCLILI
                     SIALQRLDGREIYILNKNLQKLNLELEGRVQARTAELTVVNADLEKAMRAKDEFMAAM
                     SHELRTPLTGILGGAGALQGSTDDTLSDKQKKSVNLIERNGHRLLSLVNDLIDYSRLQ
                     SGKQILFKSPYALTDICIAALRSVETDAKRKKQSVSLQSQPEGIIVDTDVTLMKKMLI
                     HLLRNASKFTPEGGEFGISLTEKSEQHCVEVVVWDTGIGIKKSDIANIFTPFVQLDAT
                     LARKYEGTGLGLAIVNKLVELFDGKMDVFSTPGSGSRFVILLPWKGPTEQ"
BASE COUNT         3945 a         3494 c         3303 g         3626 t
ORIGIN      
        1 gacaggggac tttttttatg gaaattgatc caaaaattat ttcaatatgg ataaggtctt
       61 tcgcaaataa aaaactctta cgaaattatt tattcatcgt cacgtgatgc atgttagtaa
      121 agaatgttgc cagaaaacga aataaacatc catataacat ccatttggat gtaaaaaact
      181 tgacaattcc aaaaaaacac aataaaatac aaaaaatacc atccatacag atggtaaagg
      241 agagaaatga cggaaacaga gaaaattaca ataaacttag gtgccgtaga tttaggcaag
      301 attgatctgc tggtcgaaca aggacactac tcgaaccgca cagacttcat ccgctcggcg
      361 attcggtcac aactagataa gcacgtgctt gaggtacagc aagcagtcac ccgctattcc
      421 tttggcgtcg gtgcattctt tcatagccgt gcagactttg aaaaatataa agccaagggc
      481 gataagatca aaatcgatgt cattgggata taccatgttc cagatgatgt ttccgtagct
      541 cttgcgctgg aagtcgtcga gtccattaaa gtgtatggca tcttccaagc gcgtagtgaa
      601 ttaaaagacg ctcttcagaa agcagggaaa atcagatgac aagcgaacaa caagttccag
      661 aaaactgggc tatccgtttc ttcaccatct ttgcaggtca ggctttttca ttattcggct
      721 ctgcattagt tcagtttgca ttaatctggt accttaccca aaagacaggt tctgcaaccg
      781 tacttgccac cgcatcttta ttcgggatgc ttccacaggt acttttagga cccattgcag
      841 gaaccattgt ggatcgaggc aaccgtcgct tgatcatgat cctatcagac tcactgattg
      901 cgatctctac gcttcttctc gcctacttat tctggtctgg cgctgcagaa atttggcata
      961 tctatgtagc cctaaccatc cgatctgcag gcggagcttt ccactacccc gcaatggcgt
     1021 catctacctc gttaatggtc cccgataaac acctctcgcg tgtagccggt gccaaccaaa
     1081 ccttgcaagg gcttattagc atcgttgctc caccagtagg cgctctgctt gtcgcagcca
     1141 tgtctacaca aaatattttg atgattgacg taattactgc cattatgggt gtcacaccgt
     1201 tgcttttctt caccattcca caaccgcccc gtaaagctga gcaaaccgct acgcaaacca
     1261 gctttcttaa agacctcggt gcaggtttca aatatatggc atcctggcct ggtctactca
     1321 tgttaggttt actggcaact gtattgaact tcctcctcgc tcccctcaca gcgctcatgc
     1381 cattgttagt tacgaaatac ttcaaattag gtgctctcga attaggcagt ctcgactcat
     1441 tcttcggcat cggcatgatt gccggcggaa ttacccttgg ggtttggggt ggtttcaaaa
     1501 aacggatcgt cacttccctc tcgggtatca tcctcactag tttttctatc cttgccattg
     1561 caggtgcccc ttcaaatgcc ttctggatgg tggttgcagc catggcattc atgggattta
     1621 tgatgcccat ggtcaatgga ccgatccatg ccttatttca gtctgttgta gagcccgaca
     1681 tgcaaggaag agtcatgtca ttgataagca gcgttgctca agccatgatg ccaatcggtt
     1741 ttatccttgc aggtcccatt acggatgcaa ttagttttca agcatggttc tgggtggcag
     1801 gtattcttaa cctgcttatt ggggtgagcg gttttttcat cccagcagtt atcaatatag
     1861 aagagaaccg aaaagcctat catcccgctg aaagtaacga gtcaacacct ataacagaat
     1921 ttagcaatta atttattaaa ttcatcctcc aagatgatgg ttacaggtcg cgccttatgc
     1981 tgcgacctgt aactcattta aggcatagat aattcattta taataactat tctatgttaa
     2041 taaaaatcaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttgcattgg
     2161 gattagctgg attatgggca tcagggaaca ctcagtctgt aattttaaat ggactgcttc
     2221 cgcttacaga tgctgctggt tattatactg atgcactacg attgcaatat ggatcaaatg
     2281 tttccaactt tactgcgatg cgtccgctgt atgcaggatt attatctttt tttctttgga
     2341 tatcgaataa caacctaatg attgctgtat caattttcac ggcaattaca ggtttttctt
     2401 gttacttagc ttgtaaagag atccaaaaaa cacatggggc tgaagttgct gtatttttct
     2461 taattctaac ctttctatat tatcgccacc acagcggaac taccatgtcg gaaagtttag
     2521 gagttccagt aagcttgctt ggtgtagctt tactttggaa ggggatttat tcaaaaaatg
     2581 aaggaactac tttatttgga atagggatga ttgctttagc cctgaatatt cgcccaggag
     2641 ctatgtttgt tctacccgga cttttactat ggggaggttg gatatttcga aaagaaaaaa
     2701 agttttcttt caaattcttt atctctggac taactgtagt tttatctgtg ttttatataa
     2761 atagtcgcat gatagatttt gttgctggtc caagtggagt agcatttgaa aacttctcgt
     2821 gggcatttta tggcttatct tccggcggca agtcttggac ttatattttt gaagcacacc
     2881 ctgaattacg tctcctacaa gactcagaag taacacctac catttataaa ctcgctttta
     2941 aacaaatact gaacaatcca tctcttatga ttaagggttc atttttttac tggcgaatgt
     3001 ttttctctga ttcatggtac aacgcctatg cttttgtagc tggtgaaaac tattggatca
     3061 gtgaaatagc acgatggggc atgtatattc ttggagcatt agggatttac aaattactaa
     3121 aaaactatca agatccgtat acaagtctag ctggaattac agcagtagga gtactaacct
     3181 cagtaccctt cgtcccgccg acagatgcat atagagttcg gctttacgcg gcgacaattc
     3241 cttttttcat tctattacca ggaatgggag tctctctatt gctcgatgca gttcctcaac
     3301 gactcttaag ccctaaattt acttttctta ataaaaactc aacaagtgct atcttctcaa
     3361 ttgtattaat acttacactt cttctatcac caatatacat aaaaacagca ggaacctcgc
     3421 ctacattttc acaaacaagt tgcccggcta gtttagatag ttttctcata aggtatgatg
     3481 atggtagctc tttcactgtt tatagagaga gccaagtgtt tattgatcaa atgccagatt
     3541 ttcatatgag tacttttcgt cggaatgctc acagtctgcc agatatgaat ctcataacaa
     3601 ccctagaaaa tatagaaccg cctaaaacaa tcacttactc catcgactta ttaacgaaca
     3661 atcaggctat tattattgcc gaacctgaat tattgcctgt ttcaggtgta tacctgcttc
     3721 tttgcggcaa atttatttat gaacctaatt tcactccata ctccttcttt tacgtcgagc
     3781 aagtcttttc agttgaaggg cattaaactt gccaatatag aaacgcatgg tataatactg
     3841 cccgctgtta gtttggagag gtcgcgtagt ctggcttagc gcgcacgctt ggaaagcgtg
     3901 taacccacaa aggttcgcgg gttcgaatcc cgccctctcc gccagccatg cccctcaatg
     3961 aggggctttc tttttatcaa attttctcta acaaaaaagg acctgatcat gaaatcaggt
     4021 cctcttttta ttccgggtac tacggtgttg gtgtagcttt cacatcaggg agcggagttc
     4081 ctgcatatac catcaggtca gaggatagag ccgcatcatg cccagggaca tcggcatgtg
     4141 cctctacaat gaactgatac aacagggacg atttccactt cccaccgcca aggatatcgg
     4201 tacccttagg tgcatcatcc gtacggcgtg acccagcaag gatgccttca tagctgctca
     4261 tgtccaattg ggcgggagaa acagccaaat caacagagtg acaggaaatg caggagagag
     4321 agccggaata ccaaaggtta gctttagtga ataaaggctg cacatcctca aaagtggctt
     4381 cacaggttgc accattggca tctgtgaact caaaaacatc tgcttctgga gagcctgcat
     4441 ccacccaagc gccgatcaaa tcggagccag caacatagca tttatcagag ggtgccacta
     4501 tgttggattc cacagtcata ggcggaagtg tggctggaat caaggttgga acaggggtgg
     4561 tatcaggcaa agtattgccg cggttgcagg aaagagaaac accacaagca ctcacaaaca
     4621 cgatccctac ccaagccagc acgcctacca gaaatacggc gattgcaccg taaatatatt
     4681 ttcgaacatc gtctttcatt gcatgcatct cctactgact ggcttttagc gtgcggaggt
     4741 agttgatcac atcccaacgc tcgcgaatgc tgagattttc agccaaaggt ggcatttgcc
     4801 cggagaactg tcgttctggg aaaagagtgt tgttgggatt ccaaataccg ttagagatcg
     4861 tcaggaacca gctaccatcc gatttagctt gaacaggttc actagtgagg tcagcaggct
     4921 tcttctttac gaggaatgct gccacggttc cgttcccctg cccagtttcg ccatggcaca
     4981 tcatgcagtt gatctgatag atctcttttc cacgggcgat agaggtttca tctgcaggaa
     5041 ccggattggt gggctcaccc atgccaggca aatacgccgc gccatccact ggaatggact
     5101 gtgcaggaac tgccaaaggt ttttcctgat catcaaaaga gggttgaatc gccataaatg
     5161 tcacccactg gatcttaatg atgtcatatg agaatgtcat caagatggca aggaggacgg
     5221 cgagaacagc aaatacagca acaagttgtt tgaaaagtct cataggtgtt gtgcctccgc
     5281 aggcttcaca gattcagcgc caagtttctt tagcaggtct gtgattttct tctcatcgca
     5341 gtctgggcac tctacataga tagcgatctt gccatcactg atctctggca catacttcat
     5401 tggacgatag ttcgggaagt agctatcaag aaacacacca aggaaggtgg aaagaagcat
     5461 gccaagcatg gttaattcaa agagaacaac cacagtagga ggacccggta caaaagcttg
     5521 agtgctaact tggatcgggt acggatacca atagggcgcc acaaaagcga ggaaggcacc
     5581 tgcaccaaaa ccgagaattg cgccacctaa agcaatgcgc ggcacattcg tccattggct
     5641 ggggcgaccc aacatagctt cagtgaccgg aacaccagag atgacattca tgtgagcatc
     5701 actaacgccc aaggcgcgga gttgttcaat ggcgtccgca gcaggcgcga ggtccgaaaa
     5761 gaccgcgagt atatcgacaa cagtattttt cgtttcagtc atcgtatcct cctattcaag
     5821 ttcgctgaca gagaccacgg tctcacgtcc aaatttcttg agttcgtgtg ccatctgacc
     5881 ttcctgcact tcccaaacag ggataagcgg aatcaacttg gaagccgcca tataaagcag
     5941 gatgaagagg gcaacagtac cgatacccat ggggacttcg ataccaggtg tgtacatacg
     6001 ccaagtgaac ggcatacggt tgatggtgac agagatcggg ataatgatgt agcgttcgaa
     6061 ccacataccg acgttgatga tcaaaccgac aacggtgatc gcccatggtg tggagcgcca
     6121 tttggaattc caaaggatcg cccaaggcac tacaacgtta cagaacaaca tgccaaacca
     6181 catccatcca agcggaccgg cttcatggaa gtggagtaac tgctgtgtcc acttatcgcc
     6241 accataccac tgtaccatgt aatcgttgaa gaagaagtat gcccaagcca tggaaataat
     6301 cagcatgagc ttaccaatcg catcgaaatg ttcaccgcgc acaaagtaat ccatgtgttt
     6361 catggtgctg cgaattacag ccaatacaac cgcagccgca cccataccgg aatgcaaagc
     6421 acccacgata aagtacggac caaagatggt ggaagaccaa ccagggcggg tcgccgcagc
     6481 aaagtcccaa gacacgatgg tatgaacaga gaacatgacc gggatgatcg caaaggcgaa
     6541 aatgttcatc gcgttgcgga ggtgggtcca ctccccttct gtaccgcgga agccaagagc
     6601 aaggatctta tacaaattct tgcgccagcc agtggagcgg tcacgagcca tcgctacatc
     6661 agggatgagc ggcaggaaga gatacatggt ggaagaaagc aagtaagttg tgatcgctaa
     6721 caagtcccat gaaagtggag agtgaaggtt gggccacaac atgcgttcat tggggtaagg
     6781 cattagccag tagaaaagcc aaacacgacc catatgcatg aagatcgaga agccagcttg
     6841 caccaaaccg aaggtggtca tcaattctgc cgcacgggta aacggacggc gaaattctgc
     6901 ccggaacacg cgcaaaatcg cggagatgaa tgtacccgca tgactgatac cgatccaaaa
     6961 cacggtattg accaggaaga taccccaata gtctgggcgc atcacaccgg cttcaccctg
     7021 accttttgta atgaggatcg cccacttata gaagaggaag tacaccacta caaatgccaa
     7081 gaaaccaaag atcacccaga atttccatgt gggtttttcc agcatggact ccatgaccat
     7141 ttcgttcatg cgaccgcgag gaagcgggtc gagcatcaag tgtttttcac gcaggtgttc
     7201 ttcgtgcgaa tgcgctgcgt gatgtttatt atctgacata attaccctcc cttgaagtag
     7261 gtcacgtttg gaagcgtgcc aagttcttca agatgtttga cgccatgtcc tttgtgagcc
     7321 aattgagaga ccatcgattc aggctggtcc acacgtccga agacaatggc gttggcaggg
     7381 caggcttggg cgcaagcagt ggtgaattca ccatccatta cttcgcgtcc ttcggctttg
     7441 gctttatctt gcgccttgtg aatgcgctgg atacagaaag tacacttctc catcacaccc
     7501 atacgacgaa cagtcacatc agggttgaac tgattaggaa gcggttcagg gcgctgataa
     7561 tcacgccagt tgaaagcacg aacctggtag gggcagttgt ttgcgcagta acgggtaccc
     7621 acgcagcggt tgtaaacctg caagttcaac tgctcagcct gagaatggac cgtggcaaac
     7681 gccgggcaga ccggttcaca agtggcatga ccgcactgct ggcacagcat tggctgttgc
     7741 ggggtcatct tgacctcagg atattcaccc tcccaaaaac gttcaatgcg gatccagtgc
     7801 atggaacgcc catacccagc atcttcttca ccgacgaaag tgacattatt ttccattgca
     7861 caagccgcca cacaagcctg gcagccggta caaatatctt ggttgataac cattccccat
     7921 ttgccggttt cttcggcgct cattttgatt tcttcgggtc gaatcatgat taatgctcct
     7981 cacccagcgc ctcgccgtac acgcccatgc ggctttccat gcgggaaaga ccaacaccac
     8041 gtacagcgct ggagaccttt tcaactttga ctttcatacc agcaaaagca agatcaccgg
     8101 ctccgttaaa gtgagcgcca agcacatcag cggggttgac cccacgcttc tcagcataac
     8161 gcccataagc cgtatgaccc tgaccgaacg gcattgcaat cgtatcgggg cggatcgccg
     8221 gatacaagta cacatacgct tccacttcac cggcttcgct gctgacgcga accacatcgt
     8281 cattttcaat gccaagttcg tgagcggttt caggattcat ttcgatccag gtattccaca
     8341 tgaccgttgt agtggggtca gggagttcct gtaaccaagg tttgttcgcg cccgcctctg
     8401 cgagagtcgg agagacgaac ggcacgaaaa agaattcacc ttcgccagca aattcaggct
     8461 cagcggcttt gatattgcta tcaagcacag cagagccatc tggcgctacc aaagagtcgg
     8521 cgttcttata ccaaccgcca tactgctgga agtacgccat gaaggttaca gggtctgtag
     8581 cagtgaagga gccattcgca tcactcagca aagagctgat cttggtctga atgaactcaa
     8641 cttcatcttt atatggaagg gcctcggcaa acttgccgcc agccaattga gccgcagcaa
     8701 ttaacacatc agccgtggcg cgagtatcgt agaagggaga aacaacgggc tgtgcgccag
     8761 agagcaccgg caaagacacg ccagtggcta cacgctggta accccaagac tcaagtccgt
     8821 ggtggtcggg caacacatat tttgcctcag cggcggtttc atcggggaag gttgcaaacg
     8881 agataagttc tacattcgca agcgcttctt tgaagccgag agaagcaggc aattcaaaga
     8941 ccgggttcac accgtgaaca aacaaggctt tgatcgcgcc gctcttcaac tgttcgataa
     9001 aagcagccat ttcttcggca gaagcagggc ggctatattc ggtctggttc ggggcgagag
     9061 cagaaatgta cacgccaccg ggtttaccga agttatcaga gagagcattc aacgccaaca
     9121 ccgcttcggc aatttccaaa ccgttggatt gaccaagggc aggaccgcca ggaatggcaa
     9181 gcgcgtttga attggcaaac atttcagcga ggtgttccaa cgccgccaac tcgacgccag
     9241 ctgcttcagc gacttcagcc ggtttcacgc cggagaagac gcgcggtgtt gcgccgccgc
     9301 gaagctctga gacgagttta ccgattgcaa gggcaaccaa gccttcggtc ccggggcgaa
     9361 ccggcaccca atggtcagcc ttggcaccag tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nccatagcgg acaggagcat tcatgccggt ttcttttgca aggtcagaga
     9541 caaggtcgaa gaggtgatct ggtgccatac ccatgaggaa agcgatctca ctgggtttgt
     9601 aggttttgaa ggcatcggct acggtttgaa tagcagagtc ccaatctccg gtggaaggac
     9661 cttgaacacg gtctgggttg tataagccgt gaagagtggc ttgcccgcgt gcacaggtct
     9721 taccgagatt gaggggatga ttggcattgc cttcggtctt gagagcacga ccctgatagg
     9781 tgcgcaccac gagaccacat cccgccgcac attcgcggca tgtagtggcg taataggtac
     9841 tctgcccgac cgaattgtat tcaggcatct gctgataagg ttcgcgctta acataacgcg
     9901 aagcgggacc acaacctgtg aggatcgcag tggtcgccgc tcctaaacca gccagtttta
     9961 gaaaatcacg ccgggaaaaa ttttcgctca tataggtttc ctttcacccg gtttaatagt
    10021 gacacgttcc gcagtctgtc agtttgatca atttggcttg atcttcaccg gcattctgga
    10081 tgtgacaatt caaacaccag cccatgttca tcacttgtgg attttgggca atggttactt
    10141 gagtcatatc gccgtgacat tcttcacagt tcttccctgc cgcaatatgc gcacgatggt
    10201 tgaactgtac aaagtctggc acgatggcaa cggggaccca ttcaagcgat gtgccactca
    10261 ggactgcgtc cttaagtggg gcaagcaaag cactatcacg ggtcttaacg atctgattgt
    10321 ggcatgccca gcactttgct tgtgatggaa tgcccgccgc aggacccttc gccgcgcccg
    10381 gatggcaata caaacattga ataccgaagg caacatgggt cttatgaggg aaccctggaa
    10441 caggttgagc aggtgcttgt tgggtagtcc ataacccata agcagccgcg ctaaacactg
    10501 ccaggaacac gatccccacc gcgatcagac caagaggctg tttcagccaa tcgaaaaact
    10561 ttttgatcat ggatgctcct gaaataaact aagcgtaacg ccgccattgt tgccaatggt
    10621 ggcgtttgca aaattaccaa cttcttaatg acaaacatgt agcgcttttg ctggtctctc
    10681 tctttataaa aagaacccac ccaaattact ttacaagaca gccgcgctct gagggtattt
    10741 ttcgtgcatc tgcctgcact gatgcacaac ttgtaaatta tacatcgaac gtgcaaaaag
    10801 gtacaagtta tccgatttgt gttttaagcc caaacaggac ttttttggtc aaaatttctc
    10861 aacttaacgc actttaatat tgatgtgatt ttgatgttat gttttaaaag aacactccga
    10921 acaaaagaat ggtttcacct tataatgaac ctacgatgaa aacaaaaaat attttcaacg
    10981 cgtgcatttt tttgatagcc atcctaactc tcacgttggt gatcacaaac tttaacatgc
    11041 ctgtagctgc cgccaaagat ttgccggtca ctgttttaca gcaagccacc cctacccctc
    11101 ccgttgacag ttcagagatc ggctccacaa gcggaatcct ggtcatgggt gtggtcatcg
    11161 ttctgatcat cgctgtgccg ttgatcctcc aaaaaagaaa ataaaacgga ttggcttgaa
    11221 gttcaagcca ttaacctcac cttaacagat cagggattcc tttatggtat agtgccactc
    11281 atgcctgaaa agatattgat cgttgaagac gagatcgctc ttcaagaaac ccttgcctat
    11341 aacctaaaaa aagagggcta taccgttgaa accgtaggcg atggtcgctc tgcgctcgaa
    11401 gctgcccgta aactaaaacc agacttgatc gtgctcgaca ttatgttgcc agaaatggat
    11461 ggcttcgaag tggcgcgcac attacgcaaa gaaatgacaa ctggcattct gatgctcacc
    11521 gcccgcgatg acgagatcga ccgtgttgtt gggctagaag tgggcgccga cgattaccta
    11581 accaagcctt tctcgatgcg tgaattgata gcacgcgtca aagcgcaatt acgccgtgcc
    11641 cgcctattaa gagaagagat ggggaaatct gtagaagaaa cgccacacga aaccctcacc
    11701 tttgataacc tcatcatcaa cctcacccgc cgcgaagtga cattaaacgg cacccccgta
    11761 caactgaagc caaaagaata tgagcttttg ctctttcttg ccgaacaccg ccggcaaatg
    11821 ctgtcacgtg aatttattct ggaacgcgtt tggggttggg actatattgg cgatagccgc
    11881 actgtcgatg tgcatatacg ttggttgaga caaaagatcg aagaaagttc aagtgagccg
    11941 aaacgtattg ttaccgtccg tggcggcggg tatcggtttg aaggatgaag tatgaggagc
    12001 aacctacggc tctctcatta cgtattgtga cccacgtatg aatcaatttg cttatgcaac
    12061 agctatcgta tttctcatca ttgcggcgtg gttcgcatgg aagtattaca gcctgagaaa
    12121 aaacgtcaat cagttggcga aatctctgca tgaccctaaa attccgctcc cccatttaag
    12181 agaaattgaa gaactgctca gcatgcttga gtcgcgcaag gcaactttct ctgctgagct
    12241 tttctctctg caaacagaaa atgaacgcct tgcgacagtc ctcgaacagc ttaccgatgg
    12301 cgtgctcatt gccgatgccg atggccgggt gcaatttgca aaccctgcgg caagcaaact
    12361 ctttggcact cacaacccca cacaacaaag cgtggcacag gtggtgcgca gtcatcaatt
    12421 aattgaagcg tggaagcgct gccagcaaac ccgtcaactt caaattgagg cagtagaaat
    12481 cgccacacgc aaacagtatt tgcagatcat cgtcattcca gaccgccacg aaggtgggag
    12541 tttattgctc gcgcaagacc tcactcaggt gcgcaaactc gaaaccgtac ggcgggactt
    12601 tatctcgaac gtctcccatg aattgcggac gccgcttgct tcgctaaagg cgctcaccga
    12661 gacattacaa ggcggcgctc tcgcggaccc ggaagctggt ccgcgtttct tagaccgaat
    12721 ccacactgaa gttgacgcgc tgactcagat gacccaagaa ctgctcgacc tttctcgcat
    12781 tgagtctgga caagtgcagc tcaactttga aatgatctcg ccgcgtaaac tcatctactc
    12841 tgccgcagac cgaatgaaag cacaagtgga acgtgcgcat ttgaaattag atgtaaagtg
    12901 tgaagatggg ttgcaaaata tcaaagccga taaaaatcgg ttggaacagg tgttggtcaa
    12961 cctgattcac aatgcggtca aattcaataa gcctgggggg agtatcactc tgagtgcaga
    13021 gccaacttcg ggcggggtac gatgcgcagt gcgggataca ggtgtaggag tccctatgga
    13081 gagtctcaat cgcatctttg aacgcttcta tcgcgttgat tcatcccgca ccggttctgg
    13141 cacaggatta gggctttcga tttcaaagca tattatcgaa gcgcacggcg ggaagatttg
    13201 ggcagaaagt gaagagggac gcggcagtac atttttcttt attgttccgt tggacccttc
    13261 caaggcaaaa gaataacaaa acgacttcca gaaccggggg tgctaaaaac gtccatcttg
    13321 ccatcaaaca actccacgag tttatttaca attgcaagcc caagccccgt gccttcatat
    13381 ttacgcgcta atgtagcatc taattgaaca aagggagtga agatgttggc aatatcactt
    13441 ttcttaatgc caattcccgt atcccaaacg acgacctcaa cacagtgttg ttcagatttt
    13501 tcggtcagcg aaatcccaaa ctctccgcct tcgggggtga atttactggc atttcgcagc
    13561 aaatgaataa gcattttttt catcaatgtc acatcggtgt ctacaataat gccttcaggc
    13621 tgagattgta acgaaacgct ttgcttcttg cgcttggcat cggtttctac agaccgtaat
    13681 gctgcaatac agatatccgt aagagcatat gggctcttaa acaaaatttg ttttccgctc
    13741 tgcaaacgtg aataatcgat caggtcgttc acgagagata aaaggcggtg tccatttctc
    13801 tctataaggt tcactgattt cttttgttta tcgctcaacg tatcgtcagt tgaaccttga
    13861 agggctccgg ctccacccag aatgccagta agcggggtgc gcaattcatg actcatggca
    13921 gccatgaatt catcttttgc ccgcatggct ttctccaagt ccgcattgac cacggtcaac
    13981 tcggctgtac gtgcctgaac acgcccttct aactccaggt taagtttctg aagatttttg
    14041 ttcaaaatat agatttcgcg cccatccaaa cgctgtaagg caatcgagat caaaatcaaa
    14101 caccccaccc aaaaactaac tgcttcaaaa ctaataaatt ggttactctg gtgtccatat
    14161 acaaagagac aaaatgacaa aaataagagc acatctgaga agagccgatg aagattcatc
    14221 acccaatatt tagtaatctg aaccttgtgt aattctaccc atgtagacca ctggtataag
    14281 gcagccgcac cgataaaggc agaactaagg ttatatgtca aatctgtgaa gcggaccgtc
    14341 tcaaagtata gaccttgaat atcctgcaca aggaagatcg tatcgctaag tattaataaa
    14401 aggatgccca atccaagttg tatttttgtg ctaattgaaa gatagacact ccgatgagtt
    14461 acgagaatcc acaatgccat aaacaatatg gtatctactg atagatacgc tactgtaaag
    14521 aacaagtagg tcttatctga tgtccctgtt tggtcaataa ctggtctt
//
LOCUS       DAOQNJ010000005        13925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_1826_length_13925_cov_8.999279, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000005 DAOQNJ010000000
VERSION     DAOQNJ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13925
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1826_length_13925_cov_8.999279"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLG52_00305"
     CDS             <1..463
                     /locus_tag="PLG52_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39906.1"
                     /translation="QIGFGLPYAPYYFTYKNPFAKEAATYGYGEGFSEAADYLAQKPN
                     AKELTAYVYNGMGTFSYFFPGKTIVLKRANVLYGDFVTITDELRNSDYLVLYPIVREK
                     QPETEKIFSELEGVVKPEKVIYINGLEYIQIYKVTDIPETVYEKLLAKNEP"
     gene            644..4636
                     /locus_tag="PLG52_00310"
     CDS             644..4636
                     /locus_tag="PLG52_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB39907.1"
                     /translation="MNFNSSNGRFSEKSIGRVLTGIAFLYQAIAVVVVVWALVVAAQW
                     IREPFLGSFYEHTLVFTDPAADSTGVWAFNKIVNSGDQLIAINGTDVKNSHDVREVLS
                     SFVPGENVTATVKFKNGETRDLSVELQRFPEDAVSIYLILPSIISFVFLVLSIWTFGL
                     RRNEPSGRAFALFASSFAIVTGTIFNLWTSHEFTTIWTAAVAIAGGANIALALSFPAS
                     PRLIINRPYLRWMGFVLSFVLTAITIPYLFNSQNPTEYIAKWQNLYYFAAVGILSLLG
                     SNLYYSLYSQSPVVKLQARTTLIGAVFAFLPLTVYLVFGNIFSIKFSPWLFLPVVIFP
                     AIMGYNIMRFRFLRTDDLARRGVMYVLLTAIVTMGYALVAAGAAFLFGNSLPQNYLAG
                     FFILVLAIVLEPLRIRLQNLTDVLFFRGSRVVAEQLEDFSHKLTTALDLNAISSLLRD
                     QIASTLAPDQIHIFTYDSLNDFFVASPGDGRRTTTDIRFTSASPLVSYFRSERLPLYL
                     DNGTDLPDVLKPEQSRIALLGARLFIAFPGKDKPNGWLALGPRLTNQAYSPSDLRFLE
                     NISDQASVAIERVQTVAHLERRIQEMNALTRVSQGVNVTLTFDDVLELIYAQTAQIVP
                     TSHFHITLYDKDSDYYYYGFCLENNERMHERENQPFSSNVGLSPDVIRKSRPIITQDY
                     IRECQARNHTPTIENIFAWIGVPLNAGAESIGALSVGSRDASTIYTRAQVDLLQAIAD
                     QTAGAIVKARLLKETQERAHQLTTLNEITRQLTSTLELNPLLQNILENAVGILNCEAG
                     SLLLVDDQTDELVFRVTVGPVAGNLIGKRLARGTGIVGRAVETRKPVIENEEQTSNRF
                     NGVDQQTGFVSRSLLAVPLQAKDRVIGVVEVINRRDGLPFVQNDQTLLTAFAGQAAVA
                     IENARLYTLTDQELANRVEELSVMQRIDRELNASLEMDRAMRITLDWALRQSGADAGL
                     IGTLEETKLRVMAHQGLDEQMASLPDQVMKIELPSMLAAVETGSPQNVEVASGASKNK
                     LLMASQTQMVIPIRRETMVIGLLYLESSSGSQVDIDFLTRLTDHAAIAISNAQLYSEV
                     QRANLAKSDFVSFVAHELKNPMTSIKGYTELLASGAVGQITEMQTNFLGTIKSNVERM
                     STLVSDLNDNSKIEAGRLRLEFKATQASDLVDEVIRTFKRQLEDKKQTLELSIPPNLP
                     AMWADRIRVGQVLTNLVSNAFKYTPESGAIQVGVEESPNHWDANGAPRVIHLWVKDSG
                     IGMTPEDQQKIFQKFFRSDDPKAREAPGTGLGLNITKSLVEMQGGQIWFESEYRHGTT
                     FHFTVPVAEE"
     gene            4643..5803
                     /locus_tag="PLG52_00315"
     CDS             4643..5803
                     /locus_tag="PLG52_00315"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNB39908.1"
                     /translation="MAIVSSVGTAQALDGREAGLQATHQALNKLGANVPGLAVVIASH
                     QYQAREVLNGVSSLLGDTPMIGFSSPAGLTRDGLHPHSVVVALLSGDFQAETLWLPGY
                     AQSGRETAQKIEQHASARVERQSVLFFADGFNGDAEQFCNSLPQALDVTGALSSGDLH
                     TGNTYQLMGAQTGAGCMTTAFLRGNLHIGVGSDHGWDPVGNQFRVTRSRGFWLRTLDG
                     RPASEAYAGLFGQPARDWAFPPLSYLARLYPLGMEQSDGMVVRAPIRVEADGSFRMNA
                     VIRDGVDAFLLVGSRASCERAAKTAAQHALLKLGDAKPRLALVLVDVAWQMLLKAQPG
                     AEIAAVQEILGDNVPVAGGYTLGQVVTGDNETKPKFLNQHIIVIVFGEPKEQ"
     gene            5947..8577
                     /locus_tag="PLG52_00320"
     CDS             5947..8577
                     /locus_tag="PLG52_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNB39909.1"
                     /translation="MTDKKAPTKKPTRKQTPEQDIQAGDSSVAVGGSVTDSAIIHGQT
                     IVLADRFWRDLKPALPPDAIKEATAAFLAYLTDRHYYLSLKGMGVSDRVPLRLKLLDL
                     YVPLKARMELPEGETWKRDLSLAGRDIAEYEEPLHFGDPVPLLNVLKSYSGVVVLGDP
                     GAGKTTFVKYLAVRLARGEGAKMGLDDYLPILLPLAAFANALQSDDIRLDDFIADYFS
                     GIGADLPIGPMLKEALKAGRALILLDGLDEVRDINMRNTVVERVVDFYTFHRHQGNKF
                     VLTSRVVGYRAVRPSAEDLAECTIVDFEDDEIEDFVERWTTALEKQAQGHTQVAQADA
                     EADRRDLLDAIQHNAGVRQLASTPLLLTILALMKRQGVTLPERRVQLYDQYVSTLLST
                     WNRARSLSGRAPGRDIDEIQTVRILAPLALWMHEVSPGVGLVGREDMRRKLEELFHER
                     GDLSPIQAARQFLMDVREHAALLLERGPGEYGFIHLTFEEYLAAVALALMGQGNSQPI
                     IETLSRHVGEQAWREVTLLTIGYLGIRQQLPKIAGEVVDALVDVQPGPPGEAVVLAGD
                     AVLDTYPNGVPLKSRDHVIEALVNTMQDGNIRPELRRHSGILLGRLGWRPGDLDRFVE
                     VPAGEFQFGVRKEPKNIPQMYFIAKYPVTNIQYARFVKEDGYQNREFWSDLGWEWRTG
                     KFDQRTMEAIERDWLEHRPLAKRSTPYYWHNIDVSNPIVPVVGVCWFEAEAYCKWLSK
                     KIVAVPEGYTIRLLTSEEWERAARGTDGREYPWGDGFIKSAANTWDSDATGSGLGGST
                     AVVTFAQGSSPLDVWDMSGNVWEWTGSWYDEDRKYRIVRGGSWIGYQWFARSSFCNWS
                     IPLMFNDDLGFRVAIAPKIA"
     gene            8586..8981
                     /locus_tag="PLG52_00325"
     CDS             8586..8981
                     /locus_tag="PLG52_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39910.1"
                     /translation="MAQKSKQGSHVSAGDGSIVIGGDVSGSNVSLFNNNVKLTPLFKT
                     IEKAVEKNNNLAPSDKADVTAELQEIRNELESTAPDETFLARRFRNIKRMAPEIVEVA
                     FETLKNPVGGVAEVVKRVAAKMAEDANAK"
     gene            9031..9711
                     /locus_tag="PLG52_00330"
     CDS             9031..9711
                     /locus_tag="PLG52_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39911.1"
                     /translation="MKRTFFSLLLVLTLTACFAPVDALPAPTAIASDTPQMAIPSPTI
                     LPATAIPAVDTPTPQVATSGFCQDARNLQLIKDLRTAIQNRDGELLATLVNPTTGVGV
                     RFIRNGNVITYFDNIKFIFETTYAADWGLAAGSGEPVKGSFQEIVLPSLDRAFTANAV
                     ITCNELQVGGATYTPEWPYADMDFYSVHTPGTDQYAGMDWETWAVGLVQQDGNPMLAA
                     LIHYAWEP"
     gene            9854..10273
                     /locus_tag="PLG52_00335"
     CDS             9854..10273
                     /locus_tag="PLG52_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNB39912.1"
                     /translation="MSCADEYTPQLRARGYRMTSQRLAILHVLHHTGKHLSPVEVYDQ
                     ARTELPSLTETTVYRTLEFLAENGLVRPALTSSGHLAYEIARHEHHHLVCRTCGSEME
                     VEHELLKSMYETLETESGYKLTDSHLTFFGLCPECQK"
     gene            10288..11226
                     /locus_tag="PLG52_00340"
     CDS             10288..11226
                     /locus_tag="PLG52_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNB39913.1"
                     /translation="MLYSPVPMHIPDGFLSLVVSIICWVVTALIVGAAISRTNRSLGE
                     KQIPLMGVMAAFIFAAQMINFPVAGGTSGHLLGGALAAIVLGPWAGMLVMTAVIAVQA
                     LLFQDGGLLVMGANILNMGLLTAAIGYGLYRSVAGQSKSVKLAVAGVAAWLSVMAGAL
                     LTSIELWLSGTSNLQIVIPAMLGVHALIGLGEALITVSALAFIMQTRPDLLGAGSESA
                     KGSPTWAYVGGAIALFVVLLSPFASADPDGLERVATDMGFINAGQSAPYQIIPDYTIP
                     FLGETAISTIVAGVVGVIVVAAIIILVGRGMKAKSS"
     gene            11285..12076
                     /gene="cbiQ"
                     /locus_tag="PLG52_00345"
     CDS             11285..12076
                     /gene="cbiQ"
                     /locus_tag="PLG52_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNB39914.1"
                     /translation="MHFDAFDRYHEKESFLHRLDPRVKVLVTVTFIISNALLPDAAWV
                     AFAFAWLFLLACNLLSKLGITYTLKRSIVAFPFALIAITILFSMPGKPLATFHLMMSE
                     YTITDMGLLRFLSILVRSWLSVQMAILLVATTRFPDLIHAFEHLRVPAVLTTIIAFLY
                     RYLFVLTDEVFRLLRAREARSAAVAGSRSGRGVMWRAKIAGNMAGQLFLRSYERSDRI
                     YNAMVARGYAGHLYTLNPHEMKSSDYFSTAAAVAIIFITHILGRF"
     gene            12076..12831
                     /locus_tag="PLG52_00350"
     CDS             12076..12831
                     /locus_tag="PLG52_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB39915.1"
                     /translation="MPVTHITEQFSQPQSSNDVLSVNDLHFSYPDGHVALSGVSLKLY
                     EGDKVALVGPNGAGKSTLMLHLNGILGGKGEVEIAGMRLTRDNLPAIRARVGLVFQNP
                     DDQLFSPTVFEDVAFGPLHMGLPEAEVRARVDTALEAVRMTAYRDRLSHHLSVGEKKR
                     IAIATVLSMNPSLLVLDEPSAGLDPRARRTLIHLLRDLPITMLVSTHDMAMVKELFPR
                     TVVMDEGRVVADGPTSEILENETLLTAHGLEKP"
     gene            12913..13362
                     /locus_tag="PLG52_00355"
     CDS             12913..13362
                     /locus_tag="PLG52_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39916.1"
                     /translation="MIIRPDLGGRIFSGTAGAVLLILACTGLTIPFPGVLFICLGLPL
                     AVFAFGYAFGAAQTQIDEDGVSQRNFFFVPKKFTWNEIINGKIASETYNSKDSSGWTT
                     HRTRTYALFVNKEGKKIYVNANSSGPEEWWDAMRRISKEKLGERFDG"
     gene            complement(13420..>13925)
                     /locus_tag="PLG52_00360"
     CDS             complement(13420..>13925)
                     /locus_tag="PLG52_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNB39917.1"
                     /translation="KKLKVFVNPTGRFVVGGPMGDAGVTGRKIIVDTYGGMGRHGGGA
                     FSGKDPTKVDRSAAYAARYVAKNVVAAGLADRVEVQVAYAIGVAHPLSINVETFGTAK
                     IADDKIAKLIDEHFDLRPGAIIRDLGLRKPIYRKTAAYGHFGRDDIEFPWERTDKAAA
                     LKSAAGL"
BASE COUNT         3311 a         3227 c         3616 g         3771 t
ORIGIN      
        1 gcagattggg tttgggttgc cgtatgcgcc ttattacttt acgtacaaga atccgtttgc
       61 taaagaggcg gcgacatatg ggtatggcga gggtttttct gaggcagcag attatttggc
      121 acaaaagcca aatgcaaaag agttgacggc atatgtctat aacggtatgg gtaccttctc
      181 atatttcttc cctgggaaga ccatcgtttt gaagcgagcc aatgttttgt atggtgattt
      241 tgtgaccatt acggacgaac tacgaaactc tgattatttg gttttgtatc cgattgttcg
      301 agaaaaacag ccggagacag aaaaaatctt cagcgagttg gaaggggttg tgaagcctga
      361 aaaggtcatc tacatcaacg gattggaata tatccaaatc tataaagtga ccgatattcc
      421 cgaaacagtg tatgagaagt tattggcgaa aaatgaaccg taagatatta aacggcgcga
      481 tgagcgccgt tttgcttggg ctacagagta ggggatttta ggatgggaga aacaggcaaa
      541 agtccagaaa gaggcgggtt ttcctatccc accttacgcc tttgtcatga aagtaccata
      601 tgatttgggt gggattgtag agtaaaattt cagtcattaa gctatgaatt tcaactcatc
      661 caacggtcga ttctctgaaa agtccatagg gcgcgtcctg acggggattg cattcttgta
      721 tcaggcgata gctgtggttg tcgttgtgtg ggcgctggtg gtggctgcac agtggatccg
      781 tgagcctttc ttgggctctt tctacgagca tacactggtt tttacagacc cggcagccga
      841 ctcgaccggg gtttgggcgt tcaataagat tgtaaatagc ggcgatcagt tgattgccat
      901 caatgggacg gacgttaaga acagccatga tgtgcgtgaa gtactttcaa gttttgtgcc
      961 cggcgaaaat gtaactgcaa ctgtaaaatt taagaatggc gagactcgcg acctgagtgt
     1021 ggagttgcag cgcttccctg aagatgcggt tagtatttat ctcattctcc cgtcgattat
     1081 cagttttgtt tttcttgtcc ttagtatttg gacttttggt ttacgtcgca atgaaccttc
     1141 tggtcgtgcg tttgctttat ttgcatcctc ttttgcaatt gtcaccggaa cgatcttcaa
     1201 cttgtggacc agccacgaat ttacaaccat atggacggcg gctgttgcta ttgcgggtgg
     1261 ggcaaatatt gcgttggctt tatcgtttcc ggcttcacca aggcttatta tcaatcgtcc
     1321 ttatctccgt tggatgggtt ttgttcttag ttttgttttg acggcgatta cgatcccata
     1381 tcttttcaat tcacaaaacc ccaccgagta cattgcaaag tggcaaaatc tttactactt
     1441 tgcggctgtt ggtattttgt cgctccttgg atccaattta tattattcac tgtattcaca
     1501 atcgcctgtt gttaagctac aagcacgcac tacattgatc ggcgccgtct ttgcattttt
     1561 accgttgacc gtctatttgg tcttcggtaa tattttctct atcaagtttt ctccgtggct
     1621 gtttctgccg gttgtgattt tccccgcgat catgggctac aacatcatgc gcttccgctt
     1681 cttgcgtact gatgaccttg ctcggcgggg cgtgatgtat gttttgttaa ctgcaatcgt
     1741 cactatgggc tatgccctag tagctgctgg tgctgcgttc ttatttggaa actccctgcc
     1801 tcagaattat ctggcgggct ttttcatttt ggtgctggcg atcgttttgg aaccgcttcg
     1861 catccgcctc caaaacctga cggatgttct attcttccgt ggttcgcggg tggtggcaga
     1921 acagttggaa gacttctctc ataaacttac caccgctcta gatttgaatg ccatcagttc
     1981 gctcctgcgg gatcagatcg cctcaaccct cgccccagac cagattcaca tctttacata
     2041 cgactccctc aatgatttct tcgtcgcctc tcccggcgac ggacgccgta ctacgactga
     2101 tattcgtttt acatctgcaa gcccattggt gagttacttc aggagcgaac gcttgccgct
     2161 gtatttggac aatggtaccg acctgcctga tgtgcttaag ccggaacagt cgcgtattgc
     2221 gttgttgggt gcccgtttgt tcattgcatt ccccggtaag gataagccga atggctggct
     2281 ggctttgggt ccgcgcctga caaatcaggc atattctcca agcgatttgc gcttccttga
     2341 aaatatcagc gaccaagcat ccgttgctat tgaacgcgtg cagacggtgg cgcacttgga
     2401 acgccgaatt caagaaatga acgcgttgac ccgcgtttca caaggcgtaa acgttacgct
     2461 gacctttgat gatgtcttgg aattaatcta tgctcaaacc gcacagatcg ttccaacttc
     2521 tcacttccat atcacgctat atgataaaga cagtgattat tactattatg gtttctgtct
     2581 tgaaaataat gagcgtatgc acgaacgtga aaaccaaccc ttctcgtcca atgttgggct
     2641 ttctccagat gttatccgca agagccgccc gatcattact caagattaca ttcgtgaatg
     2701 tcaggcgcgg aatcacactc ccaccatcga aaatatcttt gcatggatcg gtgtgccgct
     2761 taacgctggc gctgaatcga ttggtgccct tagtgttggt agccgtgatg cctccacgat
     2821 ctatactcgc gctcaagtag acctattgca ggcgattgca gaccaaaccg ctggcgcaat
     2881 cgttaaagcg cgtttgctga aagagactca ggaacgtgcg catcaattga caaccttgaa
     2941 tgagattacg cgtcagttga cttcgacgct tgagttgaat ccgcttttgc agaacatttt
     3001 ggaaaacgct gtaggcattt tgaactgcga ggcagggagt ttgttgctgg tcgatgatca
     3061 aacagatgaa ctagttttcc gcgtaacggt aggacctgta gctggcaacc ttatcggcaa
     3121 gcgtcttgct cgcggaacgg gcattgtggg gcgggcggtt gaaacgcgca agcctgtgat
     3181 tgaaaatgaa gagcagacgt cgaaccgctt caatggagtg gatcaacaaa cagggtttgt
     3241 gagccgctcg ttactcgccg ttccactgca agcgaaagac cgcgtaattg gcgtggtgga
     3301 agtcatcaac cgccgcgatg gtctgccctt tgtacagaat gaccaaacct tgttaactgc
     3361 gtttgcgggt caggctgcgg ttgctatcga aaatgcgcgt ttgtataccc ttacagatca
     3421 agaacttgcc aatcgcgtag aagaactttc ggttatgcaa cgcattgacc gtgaattgaa
     3481 tgccagcctt gaaatggatc gtgccatgcg catcacactc gattgggcgt tgcgacaatc
     3541 tggtgcagat gctggtttga tcggtacgct cgaagaaaca aaattgcgtg tgatggcaca
     3601 tcaaggcttg gacgaacaaa tggcttcgct acctgaccag gtgatgaaga tcgaattgcc
     3661 gtccatgttg gcggctgttg aaacaggttc accgcaaaat gtggaagtag ccagcggggc
     3721 ttcaaaaaat aaactactga tggcatcaca gactcaaatg gtgattccca tccgtcgtga
     3781 aacgatggtg attgggttgc tgtatttgga gagttccagc ggctcgcaag tggatatcga
     3841 ctttttgacc cgccttaccg accatgccgc tatcgcgatt tcgaatgctc agctgtatag
     3901 cgaagtgcaa cgcgccaacc ttgccaagag cgacttcgtc tctttcgtgg cgcatgagct
     3961 taagaaccca atgacctcga tcaaaggcta taccgagtta ttagccagcg gggcggtggg
     4021 acagatcacc gaaatgcaaa ccaacttctt gggtacgatc aagtcgaatg tggagcgtat
     4081 gtccacattg gtatcggacc tcaacgataa ttccaaaatc gaagcgggtc gcctacgctt
     4141 ggaattcaaa gccacacaag cttctgattt ggtggatgaa gtcattcgca cctttaaacg
     4201 tcaacttgaa gataagaagc aaacccttga attaagcatc ccaccaaact tgcctgccat
     4261 gtgggcagat cgcatccgcg tgggacaggt gttgaccaac cttgttagca atgccttcaa
     4321 atacacacca gaaagcggcg ccattcaagt tggtgtagaa gaaagtccga atcattggga
     4381 cgccaatggg gctccacgtg ttatccattt gtgggtgaaa gacagtggta ttggtatgac
     4441 acctgaagat cagcagaaga tcttccagaa attcttccgt tccgatgacc ccaaggctcg
     4501 cgaagcgcca ggcacaggtc tcggtttgaa cattaccaaa agccttgtag aaatgcaggg
     4561 cgggcagatt tggttcgaga gtgaatatcg acacggcaca accttccatt tcactgtgcc
     4621 agtggcagag gagtaatcag cgatggcgat agtctcttct gttggaaccg ctcaggcgtt
     4681 agatggacgc gaagccgggt tgcaggcaac acatcaggcg ttgaacaagc ttggggcgaa
     4741 tgtccccggg ctggcagtgg tgatcgcctc tcatcaatat caagcgcgtg aagtgttgaa
     4801 tggcgtatcc agtttgttag gcgatacgcc gatgatcggg tttagctctc ctgccggatt
     4861 gacgcgtgat gggcttcatc cacactcggt ggttgtggca ttactcagcg gtgattttca
     4921 ggcagagacg ctttggttgc cggggtatgc tcaatctggg cgtgagacgg ctcaaaaaat
     4981 cgaacaacac gcttctgcgc gcgtggagcg tcagtctgtg cttttctttg cagacggttt
     5041 caatggcgat gcagaacagt tttgtaattc acttccacaa gctttggatg tgaccggcgc
     5101 gctttcaagc ggcgatttac acacgggcaa tacctatcaa ttgatgggcg cgcaaaccgg
     5161 cgctggttgt atgaccactg ctttcttgcg tggcaacctt catatcggtg tcggctcaga
     5221 ccacggttgg gaccccgttg ggaatcaatt ccgtgtcact cgttcacgcg gtttctggct
     5281 tcgcacttta gatggtcgtc cagcttctga ggcgtatgct ggtttgtttg ggcaacctgc
     5341 tcgcgattgg gcattcccac cattgagcta tcttgcgcgt ttgtatccgc ttggtatgga
     5401 gcaaagcgac ggcatggttg tgcgtgcgcc gattcgagtg gaagccgatg gtagcttccg
     5461 catgaacgcg gtgattcgtg atggcgtgga cgctttctta ttggtgggga gccgcgcctc
     5521 ttgcgaacgt gctgccaaaa cagcagctca acacgccctt ctcaagctgg gggatgcaaa
     5581 accccgcctc gcgcttgtgc tagtagatgt tgcctggcaa atgctgctca aagcccagcc
     5641 cggtgccgag atcgccgctg tgcaagaaat ccttggcgat aatgtacccg tggcaggcgg
     5701 ctatactctt gggcaagtgg tgactggcga taacgaaacc aagcctaaat ttctcaacca
     5761 acatatcatt gtcatcgttt ttggcgagcc aaaagaacaa taaaataaag tacaaaagcc
     5821 gcccgaaagg cggctttttc gtatagtcac gtaagggctt tcccagaaac acccttcctt
     5881 gcaagatggt aaagccaccc atacccagat tattgtatac tgaatctaca cacatcgagg
     5941 ccgggtatga ctgacaaaaa agccccaacc aagaaaccaa ctagaaaaca aacaccggaa
     6001 caagacattc aggcgggaga ttcaagcgtt gccgtaggcg gttccgtcac ggatagcgcc
     6061 attatccatg gacagacgat tgtgcttgca gaccgcttct ggcgggacct caagcctgct
     6121 ctgcctcccg atgcgatcaa agaagccact gcggcttttc tcgcctatct cacagaccga
     6181 cattactatt tgagcctgaa aggtatgggg gtctcagacc gtgtgccact gaggctaaaa
     6241 ctgctggacc tctatgttcc gctaaaagcg cgtatggaac tcccagaagg ggagacttgg
     6301 aagcgtgacc taagcctagc tgggcgcgat atagcggaat atgaagagcc tttgcatttt
     6361 ggtgaccccg tcccattgct gaatgttctt aagagttatt ctggggtcgt tgtattaggt
     6421 gaccctggcg ctggtaagac gaccttcgtc aagtaccttg ctgtccgttt ggcgcgtggg
     6481 gaaggcgcaa agatggggct agatgactat ctcccgatct tgctcccatt ggcagcattt
     6541 gcaaatgcac tacagagtga tgacatccgc ttggatgatt tcattgccga ttatttctct
     6601 ggcattggtg ccgatcttcc cattggaccc atgctcaaag aagcactcaa agctgggcgt
     6661 gcgctcattt tgttagacgg actcgatgaa gtccgtgata tcaacatgcg caatacagtg
     6721 gttgaacggg tagtagactt ttatacgttc caccgtcatc agggcaataa atttgtgctc
     6781 accagccgcg tagtagggta tcgcgcagta cgcccctcgg ctgaagattt agctgaatgc
     6841 accatcgttg actttgaaga tgacgagatc gaagatttcg tagaacgatg gacaactgct
     6901 cttgagaaac aagcacaagg gcatacccaa gtggctcaag cagacgccga agcagaccgc
     6961 cgtgacctct tagatgccat tcaacataat gcaggtgtac gtcaacttgc ttccacccca
     7021 ttgttgctca ccattctggc gttaatgaaa cgtcaggggg tgaccctgcc agaacggcgc
     7081 gtacagcttt acgatcaata cgtctccact cttctctcca cttggaatcg tgcccgcagc
     7141 ttaagcggac gcgctcccgg acgtgacatc gatgagattc aaaccgtgcg tattctcgcg
     7201 ccgttagcct tgtggatgca tgaagtcagc ccgggtgtgg gattggtagg gcgtgaagat
     7261 atgcgccgaa aattggaaga actcttccat gaacgcggcg atctttctcc cattcaagcg
     7321 gcacgtcaat ttttgatgga tgtgcgtgaa catgccgccc ttctgctcga acgtggaccg
     7381 ggtgagtatg gcttcatcca tttaaccttt gaagaatatc tcgcagcagt ggctttggca
     7441 ttgatggggc aaggcaattc ccagccgatt attgaaactt tatctcgcca tgtaggcgaa
     7501 caagcttggc gtgaagtgac cttgcttacc atcgggtatc ttggcatccg tcagcaattg
     7561 ccaaagatcg ccggcgaagt cgtcgatgca ttggtagacg ttcaaccggg tccgcccgga
     7621 gaagcggtgg tgttggctgg cgatgccgta ttggatactt atcctaatgg cgtaccgctt
     7681 aagagccggg accatgtcat tgaagccttg gtcaacacca tgcaagatgg caatatccgc
     7741 ccagagctgc ggcggcattc tgggatattg cttggtcgcc ttggttggcg ccccggagac
     7801 ttggaccggt ttgtggaagt gcccgctggt gagttccagt ttggggtccg gaaagagccg
     7861 aaaaatattc cgcaaatgta cttcattgcg aagtatccgg ttacgaacat ccaatatgcg
     7921 cgttttgtga aagaagatgg atatcagaac cgcgaatttt ggagcgacct gggttgggaa
     7981 tggcgcacag ggaagttcga tcagcgcacg atggaagcga ttgaacgtga ttggctggag
     8041 catcggccgc tggcaaaacg cagcacaccg tattattggc ataacattga tgtaagcaac
     8101 ccaattgtgc cggtagtggg ggtgtgttgg tttgaagctg aagcgtattg caaatggctt
     8161 tcgaaaaaga ttgtggctgt gccagaaggc tacaccatac gtttgctcac cagtgaagaa
     8221 tgggaacgtg cggctcgcgg tacagatgga cgtgaatacc catggggcga tgggtttatc
     8281 aagtctgctg ccaacacatg ggatagcgat gcaaccggtt cggggctggg tggtagtact
     8341 gctgtggtta cctttgctca aggtagtagt ccattggatg tttgggatat gagcggtaac
     8401 gtttgggagt ggacaggttc ttggtacgac gaagatagaa aatatcgcat tgtgcgtggc
     8461 ggttcatgga ttggttatca gtggttcgcg cgttcttctt tttgtaactg gtcgattcct
     8521 ttgatgttca atgatgatct aggttttcgt gtggcaattg cacccaagat tgcataggag
     8581 taaatatggc acaaaaaagt aaacaaggaa gtcatgtaag cgctggcgat ggcagtattg
     8641 tgatcggtgg agatgtaagc ggtagcaacg tttcgctttt caataacaat gtgaaactca
     8701 cacctttgtt taagaccatc gaaaaagcgg ttgaaaaaaa taataatctc gccccttcag
     8761 ataaagccga tgtgactgcc gaattacaag agattcgcaa cgagctggaa agcactgccc
     8821 ccgatgaaac ttttcttgcc cggcgcttcc gaaacatcaa acgcatggca cccgaaattg
     8881 ttgaagttgc ctttgaaaca ttgaaaaatc ctgtgggtgg tgtggcagaa gtggtcaagc
     8941 gggttgcggc aaagatggct gaagacgcaa acgcaaaata gtacaatgaa ctggcggaga
     9001 ccccgccagt tttcttttga aaggataccc atgaaacgga cgtttttctc tctgttactt
     9061 gttctgaccc ttacggcttg cttcgctcct gtggatgcac ttccggctcc aactgccatt
     9121 gcttctgata caccccaaat ggcaattccc tccccgacta ttttgcctgc caccgcaatc
     9181 cctgcagtgg atactcctac accacaagtt gctacatcag gtttttgcca agatgcgcga
     9241 aacctgcaac tcattaaaga tttacgtacc gccattcaaa accgtgacgg tgaattactc
     9301 gctaccttgg ttaacccaac tacgggtgta ggtgtgcgtt ttattcgtaa tggaaatgtc
     9361 atcacctact ttgataacat caaatttatc tttgaaacta cttatgccgc agattggggg
     9421 cttgctgctg gtagtgggga accggtgaaa ggctcttttc aagagattgt cttgccttcc
     9481 ctagaccgag ctttcactgc aaatgcggtg attacctgta acgaactcca agtaggtggt
     9541 gcaacctata ctccagaatg gccttatgca gatatggact tttactccgt ccacacgccg
     9601 ggcacagatc aatatgccgg catggattgg gagacttggg cagtaggtct tgtgcaacaa
     9661 gatggcaacc ccatgcttgc tgccctcatc cactatgcgt gggagccgta gtaatctaaa
     9721 gacagactaa gaaaacatgg acggggcaat atgtgggggt attatcccgt ccatgtgctg
     9781 tttaatgtaa gaaaaccccg gctgtgatat tctaataact atactattgc aagaagttgc
     9841 aataaggaga gctatgtctt gtgctgatga gtacacccca caattacgcg cccgcgggta
     9901 ccgcatgacc tctcaacggt tggcgattct gcacgtgctt catcacacag gcaaacacct
     9961 ctcgccggtt gaggtttacg atcaggctcg cactgaactt cccagcctta ccgaaaccac
    10021 agtctatcgg acgttggaat ttctggctga gaatggcttg gtacgcccgg cattgaccag
    10081 ttctgggcac cttgcctatg agatcgcccg acatgagcac catcatttgg tttgccgcac
    10141 ctgtggaagc gaaatggagg tggaacacga attacttaaa tccatgtatg aaaccttgga
    10201 aaccgaaagc ggctataagt tgaccgatag ccaccttact ttttttggtc tttgtccaga
    10261 gtgtcaaaaa taaaaggaga gattgacatg ttgtattccc cagttccaat gcatatcccc
    10321 gatggtttct tgagcctggt tgtttcgatc atctgttggg tcgtaactgc cttaatcgtg
    10381 ggtgctgcga tttcccgcac aaatcgttcc ttaggtgaaa aacagatccc gttaatgggt
    10441 gtgatggcag cttttatctt cgctgcgcag atgattaact tcccggttgc aggtgggaca
    10501 tctggtcatc ttttaggcgg tgctttggcg gcgattgttt taggtccatg ggctggcatg
    10561 ttagtgatga cggctgttat tgcggtgcaa gcgttgctat ttcaagatgg cggtctgctc
    10621 gtaatgggtg cgaatatcct caatatgggg ctgttaacgg ctgccattgg ttatggtctt
    10681 taccgttctg tggcaggtca atcaaaatcg gtcaagttgg cagttgctgg ggttgccgcc
    10741 tggctttctg tcatggcggg agctttatta acctccattg aactttggct tagtggaact
    10801 tcgaatttgc agattgttat ccctgccatg ttgggtgtgc atgccctaat tgggttgggt
    10861 gaggcgttga ttacggtttc agcgttggca ttcattatgc aaacccgacc agatttgctt
    10921 ggagcaggtt ctgagtctgc aaaaggaagc ccaacttggg cttatgtggg tggtgccatt
    10981 gctttatttg tcgtattact ttctcccttt gcttcagcag acccagatgg cttggagcgc
    11041 gttgctacag atatgggctt tatcaatgca gggcaatcag cgccatatca gattattcca
    11101 gattacacaa ttccattcct aggtgaaact gccatttcta caattgtagc tggtgtagtg
    11161 ggtgtgattg tagttgccgc tattattatc ttagttggtc gtggaatgaa ggcgaaatct
    11221 tcataagttg atatagaatc gaggtgcgta aattaataat ttacgcacct cggaataggt
    11281 ttatatgcat tttgatgctt ttgatcgtta tcatgaaaag gaaagttttc ttcaccggct
    11341 cgacccgcgt gtgaaggttt tggtaacggt tacttttatt atttcgaacg cactattgcc
    11401 agatgctgca tgggttgctt ttgcctttgc ttggcttttc ttactggctt gtaacctgct
    11461 ttcaaagctt ggaattacat acaccctcaa gcgatctatt gtcgcttttc ccttcgcatt
    11521 gatcgctatt actatacttt tttcaatgcc aggaaaaccg ctcgctacat ttcacttgat
    11581 gatgtctgag tacaccatca ctgatatggg tctgcttcgg tttttgagta tcttggttcg
    11641 ttcttggcta tcggtgcaga tggcgatcct tttggtggct accacgcgct ttccagattt
    11701 gatccatgct tttgaacatt tacgcgtccc agccgtgttg acaacgatca ttgcctttct
    11761 ttatcgttat ctgtttgtgc taacggacga ggttttccgt ctgctgcggg cgagggaagc
    11821 gcgttcggca gcagtagcag gctcaaggtc tggcagaggc gtgatgtggc gtgcgaaaat
    11881 tgccgggaat atggcgggtc aattattcct acgtagttac gaacgcagtg accgcatcta
    11941 caatgccatg gttgctcggg gatatgcggg tcatctctat acgttgaatc cgcatgagat
    12001 gaaatccagc gattattttt cgaccgcagc tgctgtagct attattttta tcactcatat
    12061 tcttggaagg ttctaatgcc tgttactcat attaccgaac aattttcaca acctcagtct
    12121 agcaacgatg tgttgagtgt aaacgactta catttttcat accctgatgg gcatgttgcc
    12181 ttgagtggcg tatcccttaa gttatatgaa ggagataagg tcgcattggt gggcccgaat
    12241 ggggcgggga agtccacgtt gatgctgcac ttgaatggaa tcttaggtgg taaaggtgaa
    12301 gtggaaattg cgggtatgcg cctcacacgt gacaatttgc ctgccatccg tgcgagagtg
    12361 ggtctcgtat tccagaaccc agatgaccaa cttttttcgc ccactgtatt tgaagatgta
    12421 gcctttggac ctttgcatat ggggttgcct gaagcggaag tccgcgcacg agtggacact
    12481 gcccttgaag cggtacggat gacagcctat cgagatcgtc tgtctcatca cttgagtgta
    12541 ggcgagaaga agcgcattgc cattgcaaca gtactttcca tgaatcctag tctgctggtg
    12601 ttggatgaac cttcagccgg gttagacccc cgtgcgcgca gaacgctgat tcaccttttg
    12661 cgcgatttgc cgatcactat gctggtgtct acgcatgata tggcgatggt gaaagaactc
    12721 ttcccacgca ctgtagtgat ggacgagggg cgggttgttg ccgatggacc aacctcagaa
    12781 attctggaaa atgaaacatt gcttacggct catgggttgg aaaagccata actaatcaat
    12841 aggaaagatg ggtactcccc atcttttttt atttttacta gaatatgggc atttcaatct
    12901 caaggagatc ccatgattat cagaccagac cttggcggac gtattttttc tggaactgct
    12961 ggcgccgtcc ttcttatttt ggcttgtaca ggactaacca ttcctttccc tggcgtgctc
    13021 tttatctgtt tggggttgcc cttggctgtg ttcgcttttg gctatgcatt tggcgctgca
    13081 caaacccaga tcgatgaaga tggtgtttca caaagaaact ttttctttgt gcccaagaaa
    13141 ttcacatgga atgaaatcat aaatggaaag atcgcatccg aaacatataa ctcaaaagat
    13201 agctcagggt ggactaccca ccgtactcga acttatgctt tgtttgtaaa caaagaaggc
    13261 aaaaagattt atgtcaatgc caatagttcg ggtcctgaag aatggtggga tgcgatgagg
    13321 cggatatcca aagagaagtt aggggagcga tttgacgggt aagttgaaga tataaaaaga
    13381 gcttctgaat tttcagaagc tctttttata aagtctgtat tacaacccag cagcactttt
    13441 caaagcagca gctttgtcag tgcgctccca cgggaattcg atgtcatcac gaccgaagtg
    13501 accgtaagca gcggtcttgc ggtaaatcgg cttgcgcaaa ccgaggtcac ggatgatcgc
    13561 gccggggcgg agatcaaagt gttcatcgat gagcttggcg atcttgtcat ctgcgatctt
    13621 ggcagtaccg aaggtttcca cgttgatgga gagcgggtga gccacgccaa tggcgtaagc
    13681 aacttgaact tccacgcggt cagccaaacc agcagcaacc acgttctttg ccacgtagcg
    13741 ggcggcatat gcagcggagc gatctacctt cgtggggtct ttcccagaga aggcaccgcc
    13801 gccatgacgg cccataccac cgtaagtatc gacgatgatc ttgcgtccgg tcacgccggc
    13861 atcgcccatc ggaccaccaa cgacaaagcg accggtcggg ttgacgaaga ccttgagctt
    13921 cttat
//
LOCUS       DAOQNJ010000006        13708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_1875_length_13708_cov_7.352523, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000006 DAOQNJ010000000
VERSION     DAOQNJ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13708
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1875_length_13708_cov_7.352523"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLG52_00365"
     CDS             <1..248
                     /locus_tag="PLG52_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB39918.1"
                     /translation="VNPERQGQGIARKIIEQLLEQNPRPLYLMCIEHNGPMYEKFGFR
                     ALGYDESPRYFQRMQKLFDVAKVVRRVNESLLVMKLE"
     gene            258..848
                     /locus_tag="PLG52_00370"
     CDS             258..848
                     /locus_tag="PLG52_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNB39919.1"
                     /translation="MKNVLYTLVGVMAGFALAGLLLFISRAPAGEPIKLLPAPTKEPI
                     AVHVVGAVPRPGLYEFAEGSRVQDAIDAAGGLLASASVDTINLAALLEDGQQLNIPFK
                     AGEEPSTSNSSDDTSLVLPGATEASSSGSSQDLININTASVEELDSLPGIGPTIAQRI
                     IDYRDENGPFQTIEDILNVSGVGPSTFDQIKDLITT"
     gene            848..1396
                     /locus_tag="PLG52_00375"
     CDS             848..1396
                     /locus_tag="PLG52_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB39920.1"
                     /translation="MGFDHFSAIAPIYARVTYSKKEMMREVASLPVQGRLLDVGGGTG
                     RVSSAIREYVDEVIIADVSFGMLARAPRPSLQPVCGASETLPFADNFFERVIMVDALH
                     HVANHAHTAHEMFRVLKPGGVLVIEEPDIRTFGVKLIALAEKLLLMRSHFLAPDGIMK
                     LFDIGEKETKSVNGTAWVIIKK"
     gene            complement(1430..2578)
                     /locus_tag="PLG52_00380"
     CDS             complement(1430..2578)
                     /locus_tag="PLG52_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB39921.1"
                     /translation="MNFDLTEEQKTWKKIVHDFAEKELRPKAREVDEKEEFNWEAFRK
                     AGPVGLASMSIPEEYGGSNVDMISNAIAFEELGWGCGSTALAFTAHNGLGTAPIALYG
                     SEELKKKWLPLVGNGKNKLGALALTEPGAGSDIQGGVTTRAEKDGDHWVINGAKMWNT
                     NASIAEYIITLVRTDPKGGSKSLSMILVPTDSKGLSIAPAEKKMGLRGSPTHAVTFDN
                     VRVPLGNLIGPEGRGLQQTLSTLDAGRISIGAISLGLARAGMEAAIQYSKERKAFNQS
                     LADFQAIQFKIANMEMEIELARTYIYKAAWLKDEGRPYSKEAAIAKLYATEMAERVLY
                     DAIQIHGGYGYSREYNVERMYRDARLMTIGEGTSEIQRLVISRHVLAG"
     gene            complement(2592..2936)
                     /locus_tag="PLG52_00385"
     CDS             complement(2592..2936)
                     /locus_tag="PLG52_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNB39922.1"
                     /translation="MKNTVLLCTKFGMGQTDIASLNQKLISKYLSLLLEGNQLPAAIC
                     FYTDGVKLVTGISPVLEQLSQLEQKGVRLIICSTCLDEMGISRDDVKVGIVGGMPDII
                     EAQTKADKVITL"
     gene            complement(2940..3173)
                     /locus_tag="PLG52_00390"
     CDS             complement(2940..3173)
                     /locus_tag="PLG52_00390"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNB39923.1"
                     /translation="MDELKYDLLTELTNRMQADLLESFLEANGIDVELFQESVGHSIY
                     PVTIDLLGRVQVFVPKDKIEQAKILLEEFNAEE"
     gene            complement(3176..4003)
                     /locus_tag="PLG52_00395"
     CDS             complement(3176..4003)
                     /locus_tag="PLG52_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HNB39924.1"
                     /translation="MQSYFKRQIAFPQDHGSWVFIFSPLMVGLFAGKTFQHATIYLIT
                     AALSAFLIRQPITILVKAISGRRSAADLPAARFWLLVYGIIAAAALIGLILFGYSYLI
                     YLAAPGAPVFAWHLWLVSKRAERKQLGVEVLATGVLALVAPAAFWVGIGKYDPHGWWL
                     WLFTWLQSAASIVYAYLRLSQRDMSKEQAQATSRAAWWKMGSRALLYTSFNLLLSAGL
                     GISALVPLFIFVPFLVQWLETLWGITHPSVGWKPIQIGMRQLVVSVLWTALFIVFWR"
     gene            4121..4813
                     /locus_tag="PLG52_00400"
     CDS             4121..4813
                     /locus_tag="PLG52_00400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB39925.1"
                     /translation="MAKKKLAKKNNPQTASSQKTIQYVAGGVILLVVAFFVWQYFPKS
                     APKQDAAICEQFADIPVADQYDSAPPMKVDAAKKYFATVEMENGGQFKMELYPDKAPI
                     TVNSFVFLSCKGFYNGVTFHRVLEGFMAQGGDPTGTGAGGPGYQFVNEDSDLVFDKAG
                     VVAMANAGRDTNGSQFFITFAPADFLNGGYTIFGQVTEGMDVVNAITRRDPQQSPTFE
                     GDKIISVLISEE"
     gene            4870..5109
                     /locus_tag="PLG52_00405"
     CDS             4870..5109
                     /locus_tag="PLG52_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39926.1"
                     /translation="MTDKQSRNETHGCIVCGKLHQMLVVYNVDGTFFDLKVMSSDGKK
                     VVHSTRPLVACERHTKEEVESAVARVYGTQQEDDE"
     gene            complement(5110..5451)
                     /locus_tag="PLG52_00410"
     CDS             complement(5110..5451)
                     /locus_tag="PLG52_00410"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HNB39927.1"
                     /translation="MILTVLQYIACVLTILVGAYSLFAPKNAGKFTGLTPEGGRGITE
                     IRVVLGMFFVALGAYPILFPSDASFHMLGFAYVLVGIARLISIFVDKSSEQSNWISVA
                     SEFILGAILLF"
     gene            complement(5463..6005)
                     /gene="rsmD"
                     /locus_tag="PLG52_00415"
     CDS             complement(5463..6005)
                     /gene="rsmD"
                     /locus_tag="PLG52_00415"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNB39928.1"
                     /translation="MISGTAKGRKLKAVPGDTTRPVMDRVKESLFNILAGDVIDSNWW
                     DLFAGTGAIGIEALSRGAAFVKFTDMNRMPIETIKDNVETCKFTAQADIRRGDAFTLL
                     AGTADQQFEYIYIAPPQYKKMWSEALNLVDDNMEWLTEDGTVIVQIDPTEYEEIELHN
                     LVEGEQRKYGGTLLIFYDRK"
     gene            complement(6014..6988)
                     /locus_tag="PLG52_00420"
     CDS             complement(6014..6988)
                     /locus_tag="PLG52_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNB39929.1"
                     /translation="MTTHRERIQACFEGEQTDRTPIALWRHFPEEDQNPETLAAATIK
                     FQQTYDFDIVKVTPSSSFMVKDWGVDDEWQNNPEGSREYTKRAIQKPQDWETLKLLDP
                     TAPHLAAQLQCLKLVREGIGAETPVLQTVFNPMSQAKNLAGNDLLLEHIKKHPEAVMK
                     GLETITKSTVKFIEAVHEIGADGIFYAIQHAQGRILELDEYKNIALPYDQQVLKPAEE
                     FWCNLLHLHGKDVYFSLLRLMNFQIVNWHDRETYPSLSEAKSLYRGVVCGGIRQETLY
                     SGTPAQVKEEAANAIQQTKGKRFILGTGCVVYYQSAHENIMAAIESTR"
     gene            complement(6993..7592)
                     /locus_tag="PLG52_00425"
     CDS             complement(6993..7592)
                     /locus_tag="PLG52_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB39930.1"
                     /translation="MSHTIYPLKQISSDTLPRIAEIHMGDAGLLSKLGHPFVLKYFEY
                     AIKDTRAFGFYAQEDETGEIMGFSLASPEPSSLTSKLTEDKGWFIKNIFKVVFTRPLA
                     FLQMVISSITIRRQMYEPNSIECVYFTVDPKFRGHGLGRSLQKALMDEGRKQGYKKIY
                     ASVETWNIASLKATQANGLKIIKTFREGIYHRHRLESEL"
     gene            complement(7597..>7917)
                     /locus_tag="PLG52_00430"
     CDS             complement(7597..>7917)
                     /locus_tag="PLG52_00430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB39931.1"
                     /translation="ANVLRFARSSYAGEFLYWSVIEHAMQKGCTTFDLGRSLIGSGND
                     AFKSKWAPEKKPLAYWYWLAPGHELPSLNQKNPKFQFAIATWKRLPSFIVRPLGPYII
                     GGLV"
     assembly_gap    7918..8017
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8018..8497)
                     /locus_tag="PLG52_00435"
     CDS             complement(<8018..8497)
                     /locus_tag="PLG52_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39932.1"
                     /translation="MNIQRVDSLNYPALEPQLSYIGLDSWMNFIHDVYGHSVHRFVAL
                     DGDRPLGALSLLEVKSPVFGHYLATAPFGNYGGFAFENHEARNLLLDEAQRLAQDIKA
                     EYVSIRFDDIASPPVDSWVEHPVYSTYLINLSATPDDLLKRFSSDHRNHVRKSLKKGH
                     "
     gene            complement(8494..9327)
                     /locus_tag="PLG52_00440"
     CDS             complement(8494..9327)
                     /locus_tag="PLG52_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB39933.1"
                     /translation="MTPSPRLLLTIDYEPWFAPSRRYDSWSTEQRHDLDANISQQLLD
                     PILEILGASKATFYLVGEMVQWYSSLPEKILHAGHEVGFHCHVHRKLNSIQEIEKDLI
                     ASASWIKQYNVRGYRAPMINTVEEVYPLLERHGFTYSSSIYAPTGVVQKKNNIWEIPA
                     STLPFRKTKTDFHAPRYMDLNLILGGEFPYSSSFMSGLFRRTVFNIIERELKAGKSPV
                     IFLHPYEIITPPNYFQRFAKDLLYYPLLIPFSFNKSQFLKDLLKHFPTSTMQDYIKEL
                     P"
     gene            complement(9343..10884)
                     /locus_tag="PLG52_00445"
     CDS             complement(9343..10884)
                     /locus_tag="PLG52_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39934.1"
                     /translation="MKKISQHPVLILLVINLAIGLMTFRSYGLAWDEPLFYDYGQALK
                     YAYTPANWFSGNFDVTQSFGSSGSDHANRGPAYLLVAMPLVSFLEWLGLDLASAWHLV
                     NFLTFNLGIYLLYRLASKWISKESALAVATLFAFQPLLWGHAFINPKDIPFLTFFLGS
                     VLFGFELIDEWKAKNQYPIPKLLLASFFLGIATSIRILGPLAAILNVLYALVQGIKIS
                     ELIKRPVIWLYACISLVIMFAAWPYLWLNPLDKFVEVFVFMSDNPTQLNVLFAGSNYQ
                     AGEIPLRYFPVLLRYTLTEPTYFLIAIGILLAFWKSDNKRRLTFAILLTWFGILVAYI
                     LLRRPAMYDGYRHFLFILPPLFIFAGFAFEAMFNWLKQGWQKAILTSVILTFGILPII
                     QLHPYQYAYYNNFAGGVGGVFRKYETEYWLTCYREAVLGLDQIAETGAQLFVRREPYI
                     AAYYANSNITIRDFRTEQKDMKSGDYYLVNTRSNEDLKSLRDAPTVLEVSRMGATFCV
                     VKQVP"
     gene            complement(10890..11351)
                     /locus_tag="PLG52_00450"
     CDS             complement(10890..11351)
                     /locus_tag="PLG52_00450"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB39935.1"
                     /translation="MSKAKTSHASRVYLSQLMQPEHANHHGNVHGGWIMKLVDEAGAL
                     SCMRHAQMRVVTVAVDSLVFREPIKIGDLVTFTAEVTYTGRTSMEAEVQVTAEDPITG
                     ERTHTNTAYLVYVGLDDNNRPAPIPQLVAETEEEKARLELAQQRQKRRIQK"
     gene            complement(11354..11728)
                     /locus_tag="PLG52_00455"
     CDS             complement(11354..11728)
                     /locus_tag="PLG52_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39936.1"
                     /translation="MAQVSFIPIYSTKGDADAFLLYPYLFNRTGDWIGFVTPARDVYS
                     VLGEYVGTLTNDPRIVAKRAIDETKPRVAPPPAPKRVQLPANVPLAPMMPELSHSLVD
                     VLIDMPEKLHTIDSGESRQDMD"
     gene            complement(11740..12204)
                     /locus_tag="PLG52_00460"
     CDS             complement(11740..12204)
                     /locus_tag="PLG52_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNB39937.1"
                     /translation="MILTDSRERTRFFKFAFVGAIGAAIDFGVMNLLSHVILELPLVF
                     AGSISFICAVISNFTWNRYWTYPESRSRPLLSQLGMFFAVNTAGMLIRIPILHYLEPP
                     LLQFVEDSFHTTYLTAEFYAKNLTLATAVGIVMLWNYFVNRYWTYNDIDDDN"
     gene            complement(12201..13160)
                     /locus_tag="PLG52_00465"
     CDS             complement(12201..13160)
                     /locus_tag="PLG52_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB39938.1"
                     /translation="MRILITGAAGFLGSHLCDRLLADGHEIIGMDNFITGNPDNIAHL
                     AGNEKFQFFKRDVSNYIFVPGKVDAILHFASPASPNPQSKSGYFNLPIQTMKAGALGT
                     HNCLGIARREGSRFLLASTSEIYGDPEIHPQAETYPGNVDPVGERAVYDEAKRFAESM
                     TMAYHRFHSVNTSIVRIFNTYGPRMDLEDGRALPNLLKQALLGEPLTVYGTGSQTRSF
                     CFVDDLVDGIVKLLYSKEHLPVNIGNPVEMSILEFAEIINKVTGNTAGIKYIENARSA
                     RDPQRRRPDITRAKDILNWEPKVNLEEGIRRTIPFFKQKLGLA"
     gene            complement(13184..>13708)
                     /locus_tag="PLG52_00470"
     CDS             complement(13184..>13708)
                     /locus_tag="PLG52_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39939.1"
                     /translation="THSDWAWIPPLVWGADGNGLYFVSHAPAPAPISGEESPYFDLSA
                     ISFKNEAAVSLVNSAGMFSYPAISPIQYGSREAAFQIAYLQSIFIDQSDSSRYRLVVM
                     DRDGSNSRALFPPADKNGLEPQTPVWAPAAVEGQSGEFIAVMYQGNLWLIDSGNSNTY
                     QVTGDGLITRLDWK"
BASE COUNT         3622 a         3050 c         3337 g         3599 t
ORIGIN      
        1 cagtgaaccc tgagcgacaa gggcagggta ttgcgcgaaa aatcattgag cagttgctgg
       61 agcaaaaccc gcgcccactg tatttgatgt gtattgaaca taatggacct atgtatgaaa
      121 aatttgggtt tcgtgctctt ggttatgatg aatctccgcg atattttcaa cggatgcaaa
      181 agttatttga tgttgccaaa gtagttcggc gtgtgaatga aagtttacta gtaatgaagt
      241 tggagtagaa tcgcggcatg aaaaatgtgc tttatacgtt ggtgggtgtc atggctggtt
      301 ttgcattagc aggtttgctt ttgtttatct cacgcgcgcc tgctggggag ccaatcaagt
      361 tattgcctgc accaaccaaa gaaccgattg cagttcatgt tgttggtgct gtgccgcgcc
      421 cgggtttata tgagtttgcg gaaggctccc gtgtgcaaga tgcaatcgat gctgccggtg
      481 ggcttctcgc aagtgccagt gttgatacga ttaacttggc tgcattgctt gaagatgggc
      541 aacaactcaa tattccattt aaggcgggcg aagaaccctc aacttcaaac tctagtgacg
      601 atacttccct tgtgcttcct ggtgcaactg aagcaagctc ttctggatct tctcaagatt
      661 tgattaacat caatacggca tctgtcgaag agttagatag ccttccgggt attggtccaa
      721 ccatcgctca acgcatcatt gattatcgtg atgagaacgg tccattccaa acgattgaag
      781 atattttgaa cgtctctggg gtaggtcctt ctacatttga ccaaatcaag gatttgataa
      841 caacctaatg ggttttgatc acttttctgc cattgctccg atttatgcgc gtgtcacata
      901 ctctaaaaaa gaaatgatgc gcgaagttgc ttctctacca gttcaaggac gtttgctgga
      961 tgtaggcggt ggcaccggac gtgtttcttc tgccattcgt gaatatgtgg atgaggtcat
     1021 cattgccgat gtttcatttg ggatgttagc ccgtgcgcct cgaccttccc ttcagcctgt
     1081 gtgcggcgct tctgaaactt tacccttcgc cgataatttt tttgaacggg tcatcatggt
     1141 ggatgctttg catcatgtgg cgaatcatgc tcatactgct catgaaatgt ttcgtgtgct
     1201 aaagcctggt ggcgtgctcg tcatcgaaga accggatata cgtacctttg gggtgaagtt
     1261 aattgcttta gcagagaaac tgctgctcat gcgcagtcac ttcctcgcgc cagatggaat
     1321 tatgaaatta tttgatatag gggaaaaaga aaccaaatcg gtgaatggaa cagcttgggt
     1381 catcatcaaa aaataaaaaa gcggaggcat ggaagcctcc gcttttttat tatccagcta
     1441 acacatgcct tgaaatgacc agcctctgaa tttcactggt cccttcgcca atggtcatga
     1501 gacgtgcatc gcggtacatt cgctccacgt tgtattcacg gctataccca tacccaccat
     1561 ggatttgaat ggcatcgtaa agcactcgtt ctgccatttc agtagcatac aacttggcaa
     1621 ttgcagcttc cttgctatac ggacgtcctt cgtccttgag ccaggcggct ttgtaaatgt
     1681 atgttcgcgc cagttcaatt tccatttcca tgtttgcgat cttgaattgg atggcttgaa
     1741 aatcagcgag agactggttg aacgctttac gttctttgga atattgaatt gcagcttcca
     1801 tgccagcgcg cgccaaacca agcgagattg cgccaatgct gatgcgtcct gcatccaaag
     1861 tggaaagagt ctgttgtaag cctctgcctt ccggtccaat taagtttcca agcggaacac
     1921 gcacgttgtc gaaggtcact gcatgggtgg gggagccgcg caagcccatt ttcttttcgg
     1981 caggtgcaat gctcaagcct ttagagtcgg ttggcacgag gatcatgctc aacgattttg
     2041 aaccgccttt cgggtcggtg cggacgaggg tgatgatgta ttcggcgata ctggcattgg
     2101 tgttccacat ctttgcgcca ttaatcaccc agtgatcgcc atctttctcg gcgcgagtgg
     2161 tgaccccacc ttgaatatca gaaccagcac caggttcggt gagagctaag gcgccaagtt
     2221 tattctttcc gttgcctacc aaaggcagcc attttttctt tagttcttca ctaccataca
     2281 aagcaatagg agctgtccct aagccattgt gagccgtgaa agcaagcgcc gttgagccac
     2341 aaccccagcc taattcttca aaggcaatgg catttgagat catgtctaca ttggaaccgc
     2401 catattcttc gggaatactc atgcttgcca aaccaacagg cccagccttg cggaacgctt
     2461 cccaattaaa ttcttctttt tcatccactt cacgtgcttt ggggcgtaac tccttttcag
     2521 caaagtcatg gacgatcttt ttccacgttt tttgttcttc agttagatcg aaattcataa
     2581 taactcctaa tttatagcgt aatgacttta tccgcttttg tttgtgcttc aattatatcg
     2641 ggcatcccgc ctacaatgcc tacttttaca tcgtcacgag aaattcccat ttcatcaaga
     2701 caggtagagc aaataataag gcgaacccct ttttgttcca attgagaaag ttgctccagt
     2761 accggagaaa taccggttac cagcttaacc ccatctgtat aaaaacaaat ggcagcagga
     2821 agttgattcc cttccagtaa caggctcaaa tacttgctaa tcaatttctg atttagagag
     2881 gcaatatccg tttgccccat tccaaatttt gtacaaagta gaacggtgtt tttcatttct
     2941 tattcctcag cattgaattc ttcaagcagt atttttgcct gctctatttt gtctttgggt
     3001 acaaaaacct gcacgcgccc taataagtcg attgtgaccg gatagatgga gtgccccaca
     3061 gattcttgaa ataactccac atcaatgcca ttcgcttcga gaaaactttc cagtaaatct
     3121 gcttgcatgc gatttgtgag ttcggttaat aggtcatatt ttagctcgtc catttttacc
     3181 tccagaacac tataaataat gcagtccata gaacgctcac aactaattgg cgcataccaa
     3241 tttggatagg cttccaaccc acagatggat gggtgattcc ccaaagcgtt tccagccatt
     3301 gcaccaaaaa tgggacgaag atgaacagcg gcacaagagc ggatatcccc aagcccgccg
     3361 agagaagcag gttgaaggat gtatataaca atgcgcggct tcccatcttc caccacgcgg
     3421 ctcgactcgt cgcctgtgcc tgttccttcg acatatccct ttgactcagc cggagataag
     3481 catacacaat gctggcagct gattgcagcc atgtaaataa ccaaagccac caaccatgtg
     3541 ggtcgtattt gccaatgcca acccaaaaag cagcgggtgc gaccagtgcc aatacaccag
     3601 ttgccagcac ttctacaccc agttgttttc gttcagcgcg tttacttact agccacaaat
     3661 gccatgcaaa gaccggtgcc cctggcgcag ccaagtagat aagataactg taaccaaaga
     3721 ggataagtcc gatcaaggct gcggcggcga tgatgccata gaccagcaac cagaaacggg
     3781 cggcgggaag atccgcagcg gagcgccgtc cactgatggc tttgacgaga atcgtgattg
     3841 gttgtcgaat caggaatgca gataacgctg cagtgatgag gtaaatcgtt gcgtgttgaa
     3901 aggttttgcc agcaaaaaga ccgaccatta acgggctgaa aatgaaaacc catgagccgt
     3961 gatcttgcgg aaaagcgatt tgccgtttga agtacgattg cattttggca agcatacagg
     4021 aaaaagaatt cgaacgcaag gtgccatctc tcatgtgttt attaaggaaa gaaggtcaag
     4081 gttgttataa ttgagaaaat tttttaggag agaaatttgt atggcaaaaa agaagttggc
     4141 taagaaaaat aatccacaaa ccgcatcttc acaaaagact atccagtatg ttgctggcgg
     4201 tgtcattttg cttgtggttg cattttttgt ttggcaatat tttccgaaga gtgcccctaa
     4261 acaagatgcc gcgatttgtg aacagtttgc tgatattcct gtagcagacc agtatgactc
     4321 agctccgccc atgaaggtgg atgctgccaa aaaatatttt gcaactgttg aaatggaaaa
     4381 tggtggtcag ttcaaaatgg aactttaccc cgataaagcg ccgatcactg tcaatagctt
     4441 tgttttcttg tcttgcaaag gcttttacaa cggcgtgacc ttccaccgtg tgctcgaagg
     4501 attcatggca caaggcggtg accctaccgg caccggtgcg ggcggacctg gctatcagtt
     4561 tgtgaatgaa gatagcgact tggtctttga taaagctggt gtggtggcga tggcaaatgc
     4621 tgggcgtgat acgaacggca gccagttctt tatcaccttt gccccggcag atttcttgaa
     4681 tggcggctat actatttttg gtcaagtgac cgaaggcatg gacgtggtca atgcgattac
     4741 ccgacgtgac ccgcaacaaa gcccaacctt tgaaggcgat aagattatct ctgtattgat
     4801 ttcagaggaa taattcaaaa aagagacagt cactttcagg gtgactgtct cttttcgtta
     4861 ggagtgatga tgaccgataa acaatctcgt aatgaaacac acggctgtat cgtttgtgga
     4921 aagctacatc aaatgcttgt tgtctacaat gtagatggaa ccttttttga cctcaaagta
     4981 atgagctcgg atggaaagaa ggttgtgcat tctacgcgcc cgttggtggc ttgcgaaaga
     5041 cacacaaaag aagaagttga atctgctgtt gcacgagtct atggtacaca acaagaagat
     5101 gacgagtagc taaaacaaaa gaatcgcacc aaggataaat tcacttgcaa cactgatcca
     5161 atttgattgc tcgctagatt tatccacgaa aattgaaatc aatcttgcaa tcccaaccag
     5221 cacgtatgcg aatccgagca tatgaaacga cgcatcagag gggaagagga tgggatatgc
     5281 tcctaatgct acaaagaaca ttccaagcac aacgcgaatc tcagtaatgc cacgtccgcc
     5341 ttctggggta agccctgtaa attttccggc gttctttggg gcaaataaag agtaagcgcc
     5401 caccaaaatg gtcaacacac aagcgatata ttgaaggact gtcaaaatca tggctgctcc
     5461 ttttattttc tatcgtagaa gatcaacaac gttccgccat attttctttg ttccccttcc
     5521 acgagattgt gcaattcgat ttcttcatat tcggtcgggt cgatctgcac aatcacggtt
     5581 ccatcttcgg tcagccattc catgttgtca tcgaccaaat tcaacgcttc tgaccacatc
     5641 tttttatatt gcggcggcgc aatataaata tattcaaatt gttgatcggc tgtcccggct
     5701 agcagcgtaa atgcatcgcc acggcgaatg tctgcttgtg ctgtgaattt gcaagtctct
     5761 acattatctt tgatagtttc aatgggcatg cgattcatat ctgtaaactt tacgaatgca
     5821 gccccgcggc tcaacgcttc gataccgatt gcgcctgtgc ctgcgaacaa gtcccaccag
     5881 tttgagtcaa ttacatcacc tgctaaaata ttgaatagcg actctttgac acgatccatc
     5941 actgggcgcg tggtatctcc cggcaccgct tttaactttc tgcctttggc agttcccgaa
     6001 atcactctta ggctcatcgt gtgctctcaa tggcagccat aatattttca tgcgctgatt
     6061 gatagtagac tacgcagccc gtgccgagga taaagcgttt gcctttggtc tgctgaatag
     6121 cattcgccgc ctcttctttg acctgagccg gggtgcccga gtagagcgtc tcctggcgga
     6181 tgcctccgca taccacacca cggtataggc tcttcgcctc actcaacgat gggtatgttt
     6241 cgcggtcgtg ccagttaacg atctgaaaat tcatcaggcg caacaaagag aaatacacat
     6301 ccttgccatg caaatggagc aagttgcacc aaaattcttc ggcgggtttt aatacttgct
     6361 ggtcataagg caaagcaata ttcttgtact catccaattc cagaatccgt ccttgcgcat
     6421 gttgaatcgc gtagaagatg ccatctgcgc caatttcatg cacagcttct ataaacttga
     6481 ccgtgctttt tgtaatggtt tccaatccct tcatcacggc ttcaggatgt tttttgatgt
     6541 gttccaaaag caagtcattg cctgctaaat ttttcgcctg cgacatagga ttgaaaaccg
     6601 tttgaagcac tggcgtctct gccccaatcc cttcgcggac gagcttcaag cattgaagtt
     6661 gtgcggcgag gtgtggcgca gttgggtcaa gaagcttaag tgtttcccaa tcctgtggtt
     6721 tttgaatggc acgcttggta tattctcgcg agccttcggg gttattctgc cattcgtcgt
     6781 ctacgcccca gtctttgacc ataaacgaag aggatggcgt gacttttaca atgtcaaaat
     6841 catacgtctg ctggaattta atcgtggcgg cggcaagcgt ttccggattt tggtcttctt
     6901 cagggaagtg ccgccaaagg gcgatgggag ttcggtcggt ctgctcacct tcaaaacagg
     6961 cttgaatgcg ttctcgatgg gtggtcatgc tcttacaact cgctttccag gcggtgcctg
     7021 tgataaatac cttctctaaa ggtcttgatg attttcaatc cattcgcttg cgtggctttc
     7081 aagctggcaa tgttccaagt ttctacgctg gcgtagattt ttttataccc ctgtttacgt
     7141 ccctcgtcca tgagggcttt ttgcagggag cgtcccagtc cgtgcccgcg gaatttcggg
     7201 tcgacggtga aataaacaca ttcgatactg ttcggctcat acatctgtct gcggatggtg
     7261 atgctggaaa tgaccatctg caaaaaagct agcggacggg taaaaacaac tttgaagatg
     7321 ttcttgatga accagccttt atcttcagta agtttagatg tcagtgatga aggttcagga
     7381 gaggcaagac taaatcccat aatttcaccc gtctcatctt cttgagcgta aaaaccaaag
     7441 gcgcgtgtgt ccttgattgc gtactcaaag tacttgagca caaagggatg tcccaacttg
     7501 gagagcaaac cagcatcacc catatgtatt tcagcgatgc gtggaagtgt atcagacgaa
     7561 atttgtttaa gagggtaaat cgtatgcgac ataggactac accaatccac cgatgatata
     7621 aggtcccaac gggcggacga taaaactggg aaggcgcttc caagtggcaa tggcaaattg
     7681 gaatttagga ttcttctgat tgagtgatgg caattcatgc cccggtgcaa gccaatacca
     7741 ataagcaagc ggctttttct ctggtgccca tttggatttg aatgcgtcgt tccctgaacc
     7801 aatcaaactg cgaccgaggt caaaagtggt gcatcccttt tgcatggcat gttcgatcac
     7861 agaccagtac aagaattcac ctgcatagga tgaacgtgca aatcgaagta cgtttgcnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg tccctttttg agagatttgc
     8041 gaacgtgatt gcgatggtcg gatgagaaac gcttgagcag gtcgtctgga gtggcagaga
     8101 gatttatcag ataggtagag taaacggggt gttcaaccca gctgtcgacg gggggagagg
     8161 cgatatcgtc aaatcgaata gaaacgtact cggctttgat atcctgtgca aggcgctggg
     8221 cttcatccaa caaaagattg cgcgcttcat ggttctcaaa tgcgaagccg ccatagttac
     8281 caaaaggtgc tgtagcaaga taatgaccaa aaacgggact cttcacctca agcaatgaaa
     8341 gcgcgcccaa tgggcgatcg ccatcgagag cgacaaaacg atggacagaa tgcccataca
     8401 catcatgaat gaaattcatc catgagtcga gcccgatata acttaattgc ggttcaaggg
     8461 ctggatagtt aagagagtca actcgttgaa tgttcatgga agctctttga tgtaatcttg
     8521 catagtggag gttgggaaat gcttgagtag gtctttgagg aattgagatt tgttgaagct
     8581 aaacggaatt agcaatggat aataaagcag gtctttagca aaacgctgaa aataatttgg
     8641 cggcgtgata atttcatacg gatgcaaaaa gatgacagga cttttgcctg ctttgagttc
     8701 acgttcaatg atgttgaaaa cagtacggcg gaaaagtccg ctcatgaagc tggagctata
     8761 agggaattcg ccaccgagga tgaggttgag gtccatatag cgtggtgcat gaaaatcagt
     8821 ttttgtttta cggaatggga gagtgctggc agggatttcc catatgttgt tctttttttg
     8881 cactacgccc gtgggcgcat aaattgaaga actgtaggta aagccatgtc tttcaagaag
     8941 tgggtaaact tcttcaactg tgttgatcat cggcgcacga tacccgcgga cgttgtattg
     9001 cttgatccag ctggcagagg cgataaggtc tttttcaatt tcttgaatgc tgttgagctt
     9061 tcgatggaca tggcaatgaa aaccaacttc atgccctgca tgcagaattt tttctggcag
     9121 gcttgaatac cattgcacca tttcgccaac aagatagaaa gtcgcttttg atgcgccgag
     9181 gatttcaaga atggggtcaa gcaattgctg tgagatgttt gcgtctaaat catgacgttg
     9241 ttctgtgctc catgaatcat acctgcgcga aggcgcaaac cagggttcgt agtcgatggt
     9301 gaggagcagg cgcggagaag gtgtcatggc gcggggatta tattacggga cttgctttac
     9361 gacacaaaat gtcgcgccca tacgagaaac ttcgagcacg gtgggagcat cgcgcaaaga
     9421 cttgaggtct tcgttcgagc gggtgttgac aagataataa tcgccagact tcatgtcttt
     9481 ttgctcggtg cggaaatcgc ggattgtgat attagaattc gcgtaatatg ccgcgatgta
     9541 cggttcgcgt cggacgaaaa gttgggcacc tgtttctgcg atttgatcca agccgagaac
     9601 ggcttcgcga taacaggtca gccagtattc ggtctcatac ttgcggaaga ctccgccaac
     9661 tccgcccgcg aagttgttgt agtaagcata ttgataaggg tgaagttgaa tgatggggag
     9721 aattccaaaa gttaagatga cggaagtgag tatggctttt tgccagcctt gttttagcca
     9781 gttgaacata gcttcaaatg cgaagccagc aaaaatgaac aatggtggaa gaataaaaag
     9841 aaagtgacga taaccatcgt acatggcggg acggcgcaaa agaatatacg ctacaagaat
     9901 gccaaaccat gtcaacaaga tggcgaaggt caggcgtctt ttattgtctg atttccaaaa
     9961 ggcgagcaga ataccgatgg caattagaaa ataagtcggt tcggtgaggg tatagcgcaa
    10021 caagacggga aaataacgca gtggaatttc accagcttga taattacttc cagcaaagag
    10081 tacgtttagt tgagttgggt tatcagacat gaagacaaaa acttcaacga atttgtccaa
    10141 tgggttcagc caaaggtagg gccatgctgc gaacatgata accaaagata tgcatgcgta
    10201 tagccaaatt acagggcgct ttattagttc ggagattttg atgccttgaa ccagtgcata
    10261 aaggacattc agaatcgcgg cgagtggacc taggatacga atagaggtag cgattccgag
    10321 gaagaaagag gcgagcaaca atttaggaat tgggtattgg ttttttgctt tccattcgtc
    10381 aatcagctca aagccaaaca gaacagagcc gagaaagaaa gttaagaagg ggatatcctt
    10441 cgggttgatg aaggcgtgcc cccagagcag aggttgaaag gcgaaaagag tcgcaactgc
    10501 aagcgcagac tctttgctta tccattttga ggcgaggcga tagaggaggt aaatgccaag
    10561 gttgaaagta aggaagttaa ccaggtgcca ggctgaggcg aggtcgagtc caagccattc
    10621 taaaaaagag acgagcggca tggctaccaa aagataagca ggtccgcgat tggcatggtc
    10681 gctgccgctc gagccaaacg attgtgtgac atcaaaattt ccgctaaacc aatttgcggg
    10741 ggtgtaggcg tatttgagcg cttgaccgta atcgtagaag agcggctcgt cccacgccaa
    10801 gccataagaa cgaaacgtca tcaatccaat agcaagattg atgacgagca atatcaaaac
    10861 aggatgttga cttatttttt tcaagaggat tacttttgaa tacgtctctt ttgacgttgt
    10921 tgtgccaatt cgaggcgtgc tttttcttct tcagtttctg cgaccaattg cgggatgggc
    10981 gcagggcggt tgttgtcatc caaaccgaca tagacgagat aggcggtatt ggtatgagtg
    11041 cgttcgcctg tgatggggtc ttctgcggtc acttgtactt cggcttccat ggaggtgcgc
    11101 ccggtgtagg tcacttcggc ggtgaaggtg acgaggtctc cgattttaat gggttcgcgg
    11161 aaaacgagcg agtcaacggc gacggtgaca acgcgcattt gggcgtgacg catgcaagaa
    11221 agtgcaccgg cttcatcaac tagtttcatg atccaaccgc catggacatt gccatgatgg
    11281 ttggcatgtt cgggttgcat aagttgtgag agatacacac gcgaagcatg ggatgttttt
    11341 gcttttgaca tgactaatcc atatcctgac gggactcgcc actatcaatt gtgtggagtt
    11401 tttccggcat gtctataaga acatccacga gggagtgact gagttctggc atcataggtg
    11461 ccaacggaac gtttgcaggc agttgaactc ttttgggcgc gggcggaggg gcaacacgag
    11521 gtttggtttc atcgatagcg cgtttcgcta cgatgcgcgg atcattcgtc agtgtgccaa
    11581 cgtattcacc caataccgaa tatacatccc gagctggtgt gacgaagccg atccaatctc
    11641 ctgttcgatt aaagagataa ggatataaaa ggaacgcatc tgcgtcccct ttagtgctgt
    11701 agattggaat aaatgatact tgtgccataa cgtcctgcct taattgtcgt catcaatatc
    11761 attatacgtc cagtaccggt tgacgaaata gttccataac atcacaatgc ccactgctgt
    11821 cgcaagagtc aaatttttgg catagaactc ggctgtaaga taggtcgtgt gaaaagagtc
    11881 ttcgacaaat tgcagcaacg gcggttcgag ataatgaagg atgggaatcc gaatcaacat
    11941 gccagccgta ttgactgcaa agaacattcc caactggctc agaagcggac gggaacgcga
    12001 ttcgggatac gtccaataac ggttccaagt gaagttactg atcactgcac agatgaaaga
    12061 gatgctccct gcgaatacca acggcaattc aagaatgacg tgtgaaagca gattcatcac
    12121 gccaaaatcg atggcggcgc caatggcacc tacgaacgcg aatttgaaaa agcgcgttcg
    12181 ttctcttgag tctgtcagga tcatgccagc cccaattttt gtttgaagaa tggaatcgtg
    12241 cgccggatgc cttcctctaa attgactttc ggttcccaat tcaagatgtc ttttgcacgg
    12301 gtgatatcag ggcggcgacg ttgtgggtcg cgagcactac gggcattttc aatgtactta
    12361 ataccagctg tgttgccggt gactttgttg atgatctctg caaattccaa aatggacatt
    12421 tctaccgggt tgccaatgtt gaccggtaaa tgttccttgg aatacaacaa ctttacgatg
    12481 ccgtccacca aatcgtcaac gaagcagaaa gaacgggttt ggctgccagt tccatacacg
    12541 gtaagaggct ctcctaacaa tgcctgtttc agcaagttag gcaaggcacg cccatcttcc
    12601 aaatccatgc gtggaccgta cgtattaaag atgcgcacaa tgctggtgtt gacactatgg
    12661 aaacggtgat acgccatggt catggattct gcaaaacgct tggcttcatc atagacggcg
    12721 cgctcaccga ccgggtctac attaccagga tatgtttctg cctgcgggtg aatttcagga
    12781 tcaccataaa tttcactggt agaggcaagc aagaagcgcg agccttcacg ccttgcaatg
    12841 cccaagcagt tatgggtgcc taaagcgcca gccttcatgg tttgaatggg taaattgaaa
    12901 taaccagatt ttgattgtgg gttggggctg gcaggagagg caaaatgcag aatggcatct
    12961 actttgccag gaacgaaaat atagtttgat acatcacgtt taaaaaattg aaacttttca
    13021 ttccccgcca agtgggcaat attatctggg ttgcccgtaa taaaattatc catgccaatg
    13081 atctcgtgac catctgctag taagcggtcg cagagatgag agccaagaaa ccctgccgca
    13141 cctgtgatca aaattcgcat tatgttacac ctcttctttt tatttacttc cagtccaacc
    13201 gggtgatcaa tccatcaccg gtgacttgat aggtattgct attgccacta tcgataagcc
    13261 aaagatttcc ttggtacatc acagcaataa attcgccact ttgcccttcc accgcagcgg
    13321 gagcccacac gggtgtttga ggttccaacc catttttatc cgcaggcgga aagagtgccc
    13381 gactgtttga accatcccgg tccattacaa ccaaacgata gcggcttgag tcgctctggt
    13441 cgatgaagat ggactgtaaa taggcaatct gaaatgccgc ttctctactt ccatattgga
    13501 tcggagaaat ggcaggatat gagaacatgc ctgctgaatt gaccaaagac actgccgcct
    13561 cattcttaaa cgagatggct gagaggtcaa aataaggcga ctcctcgccg gaaatgggtg
    13621 ccggagccgg agcatggctg acgaagtata aaccgtttcc gtctgcgccc caaacgagag
    13681 gcggaatcca cgcccagtca ctatgcgt
//
LOCUS       DAOQNJ010000007        13581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_1897_length_13581_cov_8.266746, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000007 DAOQNJ010000000
VERSION     DAOQNJ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13581
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_13581_cov_8.266746"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PLG52_00475"
     CDS             <1..590
                     /locus_tag="PLG52_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNB39940.1"
                     /translation="RKTGIFTSWAECEKQVKGVVGAQFKAFESEAEADAAYLAKYEDY
                     IGKVSTSGRWREASIKPLLPSVCVDAACSGAPGTLEYRGVNTETGEEIFHSGPYADGT
                     NNVGEFLAIVHALTWQEKHNIHIPIYSDSENAISWVHTGKCRTKLAHTSKNSILFAII
                     KSAENWLAENELPEGKIIKWDTNVWGENPADFGRK"
     gene            656..2236
                     /locus_tag="PLG52_00480"
     CDS             656..2236
                     /locus_tag="PLG52_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNB39941.1"
                     /translation="MRFFGLSWSLGRLRGVDIRFHFSMLFSIPIAYYLFDPIDLRGVV
                     EALLWLVGFTIFILLHEVGHAFAAQMVGVEVKSIVVWLLGGLTNLAYKPEKPAHNFFV
                     YAAGPLMNMLLAFLCVAAYILTAMLFLPFSKDPQTYIWIQTFQNLFFSLAIVNLILAL
                     FNLIPVYPLDGGNIFHAAMEWLFGKTNADRITLVVGIPFLLVLIVFGVISRDYVLLAF
                     CVLIALSISTLNHSLLRNINLGITYLFKRAGYYYLKGDYERAAQLYTAEIEKQPDDIN
                     NYLARAGCYLAMAQRERALADVDRVLKLHPNHFLALELRGEILMLNKDYDAAFAHFKQ
                     VQHINANWAVPYFDLASLYMERGELQPAIENFNKAVVLQPRMPLFYIIRSLAHFKAGD
                     LASAHHDQDVAVGLSPEEALVMVDVNLIVYEDHWDWAKDYYERILTKNPRNAFALQGF
                     AEACLVNQKWDEAVTFFSRAIEQNPREARLYVGRGKAYVELEKLQEAKVDFEKAVASS
                     EKLHLKRQAENFLRTLDI"
     gene            2251..2820
                     /locus_tag="PLG52_00485"
     CDS             2251..2820
                     /locus_tag="PLG52_00485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNB39942.1"
                     /translation="MKTALLVIDIQKDYFPGGKFPLVEPLAAAEKAYGLLQCFRENNE
                     KHVHIQHISLKPDATFFVRGTEGSDIHDAVAHFEGEPIVYKHYPNSFRETTLFDMLKG
                     WGIERVVITGMMTHMCVDATVRAAADFGFQVWVAEDACATRDLEHNGTVVPADHVHRA
                     FLAAFKSYGQVMMSEQVIARLAAEKANAR"
     gene            complement(2890..3708)
                     /locus_tag="PLG52_00490"
     CDS             complement(2890..3708)
                     /locus_tag="PLG52_00490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB39943.1"
                     /translation="MTAFPPNAYELIRPLLLNMDIHLIGRSILAKQTPAQIFVDNHEH
                     PKALFAQAGHRYILAGDSERDSFNLGIQKLFTNVIFPREIAAGHEGFALYYDSSTWEK
                     RIDTLLSGKKTIHADREYYVCKKLKHKWQDFLPEGFEIKTVDAALLADSNLKHLETLK
                     EEMTSERPSVEDFLAKSFGVCAIYQGELAGWCLSEYNANGRCEIGIETISDYRKRGLA
                     TALTLAFLEHAFSHGITEIGWHCFKRNEASAKTALKAGFEKVCDYQSFIVLLKE"
     gene            complement(3739..4347)
                     /locus_tag="PLG52_00495"
     CDS             complement(3739..4347)
                     /locus_tag="PLG52_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HNB39944.1"
                     /translation="MNFISTLGFAGTIIDLLRAAPQLVRLLRERKSFGVSVDTSGTSC
                     VVSLGWTTYGIITNQIFVALASGAIASFFFIITVAALRLGRSAREFRVTPLWFIVLFA
                     AVTFFGKTGLGLMLSISALISNVPQIRVAYKEENLSGLSLGTWLLTLSGGLIWLIYGI
                     LQKDLTIMVSTIFQSVTSAIIIGLKLFKQRTENTTATRFISD"
     gene            4406..5533
                     /locus_tag="PLG52_00500"
     CDS             4406..5533
                     /locus_tag="PLG52_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB39945.1"
                     /translation="MQTTLDPERQKQAKEYARIRRRLWLVDTLLNAAYAIVWLVFGWS
                     ISLREWLTTITTNEWLLVALYVAIFGGIFSVFTLPLGYYSGFILPHRFDQSNQSLKDW
                     VIDRIKGLAIGAPLGLLMLELLYLALRLTGSLWWLYAAGGLLLFNVILSNLAPVLIMP
                     LFNKYVPLGDEHKDLEERLLNLAKRANTKVKGVFKFDMSKRTKAANAALTGMGNTRRI
                     ILGDTLINEFTPDEIETVLAHELGHHVHKDIPFLIISGTLLTTIGLFIASQTLNYSVT
                     FFSFNSVADVAAFPALSLILGAYGLLTQPLENYLSRWRENMADDYALVSTGKHEAFAS
                     AFTRLANQNLGEIDPEKWVVFMFYSHPPLGERIAKARKFTA"
     gene            complement(5591..6451)
                     /locus_tag="PLG52_00505"
     CDS             complement(5591..6451)
                     /locus_tag="PLG52_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNB39946.1"
                     /translation="MKIVTDCAADMSNEELKQLGIVQAPLFIQFPEGEVNSADISADE
                     FYNRLEAMRPQIPTTAQPSSGIFAELYRKLAQIDKNILSVHISSGLSGTINSARDGGE
                     QVKGEANVNFWDTLTLSGGERFQVMAAALADKAGWAMDKVQERLTKIREKTEVIYTLD
                     TLEYLARGGRIGRVQAMAGALLNLKPVIRVDTDGKYSTVAKGRSIGKSMSIIADHLVE
                     KYGNAPVWVTVLHGRFAEKADQLAEEFKQKLNVAKMEVQRISPVLGVHTGPGIVGAAV
                     VPMELMGDLV"
     gene            complement(6448..7080)
                     /locus_tag="PLG52_00510"
     CDS             complement(6448..7080)
                     /locus_tag="PLG52_00510"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB39947.1"
                     /translation="MQQRSEETRSKIIFSAVKLFSAKGYNTASVDDICADAGISKGAF
                     YHHFKTKQDLFLALLDGWLETIDNAIEASKDKTVPETFMQITEAFPYIFATANEGLPM
                     FLEFWLQASRDEKIWQASVKPYRRYHKYFTDLIKKGVDEGSFVEVDPDLASRMIISTA
                     MGLLLQSLMDPKGAKWEKVARDSTTMLVASMLKNKNTIQTTNQSNQKRKP"
     gene            complement(7240..8277)
                     /locus_tag="PLG52_00515"
     CDS             complement(7240..8277)
                     /locus_tag="PLG52_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNB39948.1"
                     /translation="MNLLEVLQFALSKVWETFSGLWPLLLLSVLISVILKLYVDQDRV
                     AAFLKRNQSNSIAMSTALAIGTPFCSCGTTAVVLGMMASTIPWAPIVAFMVASPLTSP
                     QELFYSAGLFGWPFAITFFVASIILGLTGGAIAAFAESKGWLANQGRFASDKAPRADL
                     KPAPVSQTVFQPQALLQGQAQFATQTAGISLSLPVIASEPIPTKKVVTFKQFADETFT
                     TSWKLLAMFLGFAFLGYFLNGLIPQEWIKTAFGSGNAYSVPLAAILGIPFYLNTEASM
                     PLVKAFMDSGMSAGAALAFLITGAGTSIGALAGAMTIARWRVIGIVLGTLVTGAIVLG
                     MLYDLLAPVFL"
     gene            complement(8586..8897)
                     /locus_tag="PLG52_00520"
     CDS             complement(8586..8897)
                     /locus_tag="PLG52_00520"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB39949.1"
                     /translation="MKFDPVLFAKAIADETRQKIMSECCCCELSVSEIVEKIGFSQPT
                     ISHHLAILRDANLVNVREEGKQTFYTLNQEHIAVCCGQIMVKFAPESVTTENLLKTLK
                     A"
     gene            complement(8980..10788)
                     /gene="pepF"
                     /locus_tag="PLG52_00525"
     CDS             complement(8980..10788)
                     /gene="pepF"
                     /locus_tag="PLG52_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNB39950.1"
                     /translation="MTTPLPRSKVKKNQTWNAESVFKTPEAFDTELQNILADLPKIKK
                     YQGHLGENIEMFLEAFQAIDKLEKRTAKVRVYATMASAVDANDEQGAAMSSKAMSMLA
                     QVGAATSFVEPELLSMGEAKLRQWVNEDSRMKLYEHFLNDLFRKQAHVRNAEVEELLG
                     MVRDPFGAVRNTAGMLANADFKFKPAKDSKGRKLELTQSTRSALIESADRKVRQTSWE
                     NYNDKYLEFKNTLAGNLAASIKQNVFNMKARNFNSSLEATLFNGDVPVEMFHNLLDIF
                     KKKLPMWHKYWRIRRKALGVKVLHPYDVWAPLTTKKHKVPFETAVDWICEGLAPMGDE
                     YVETMRKGCLEDRWVDWSPNAGKREGAFSTRVPSDTHPFIMMSYTDDVGSMSTLSHEL
                     GHSIHAWYASRAQPMMYYLYPSIIAETASNFNQAMTRAHLLKTKKDKYFQIALLEEAM
                     DNFHRYFFIMPTLARFELETHQRAEKGQSLTADYMNNLMADLFSEGFGGEMHLDRERV
                     GITWGTFTTHLYIDYYSFQYAIGISAANAIAKRVLDGVPGAAQDHINFLKAGSSMSPM
                     EVYKTAGIDMTSTQPIEDAFAVLEEYIDRLGELTGK"
     gene            complement(10901..12781)
                     /locus_tag="PLG52_00530"
     CDS             complement(10901..12781)
                     /locus_tag="PLG52_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB39951.1"
                     /translation="MKTIVRSLALLSIASLLLASCTLFGSSTEPAANALSEVQATQSL
                     TDLVTIELTTQYDTAAVYNTVGQTVRFKYLVKMVKNDLPDGTPANVTITGITATCPAL
                     NTINNLNDRFDPGEVLECTGDYLITQADLDKGSIANVVTANAYSVNSNTVTTTVPTVS
                     PKALTLTKTASTTTYGAANQQITFTYTIKNSGTVQLGPAQFTIADTGISNNQPFNCGN
                     ADATIAPGATLNCNATYTVTAADMNATTISHSATVSGGGVTSQPAALSLTKGSAPAAA
                     SGTTVQHTVREGEWLWQIARCYSADPNQTVSANPQVDPKNLKIGTTVTVPNVGTVGNR
                     AVHAPPELCVQLHTVQSGDTWTSIADKYGADPGLTQLSNSNNMIVGKQVKVPLYTKGL
                     NIPLSNAVATVSPTTTGMSLTVTPNTTIYTQVDQIITFNYVIKNNGSTTLGPTQFMIN
                     DALMSPTAFKCGLENSTIAPGATLTCSNAYKTTQNDLAVANLQFSTTASGTNVPTTTS
                     VATTLTKGTAQLSLTVTPSATTYNTAGQTITFTYTIKNTGSGDLGPTQFFINDPLMNP
                     ATLNCGAANTTLAPNATVQCTNNYTITDADLGAVNIQFSSSAGGTGAPTSQSVPTTLT
                     KQ"
     gene            complement(12915..13400)
                     /locus_tag="PLG52_00535"
     CDS             complement(12915..13400)
                     /locus_tag="PLG52_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39952.1"
                     /translation="MANGQVKKIATPKAAGYFIVYKVQPTDKSLKQIAREQLADEKLW
                     EKIVLDRETANPKGFFWDEIPANSFDPRWTLLLPPSEFAAFTISVNTPIHRAPNGEFL
                     AGANITEKYFYARKTVVVSGQKVWADVTKTNPARFGNKPYWILVKDGTSKTNPPISGP
                     S"
BASE COUNT         3307 a         2918 c         3490 g         3866 t
ORIGIN      
        1 gacgcaagac cggcatcttc acgtcttggg cggaatgtga aaaacaggtg aagggcgtgg
       61 tgggtgcgca attcaaagcg tttgaaagcg aagccgaagc agacgcagcc tatttagcca
      121 agtacgaaga ctatattggg aaggtatcta ccagtggacg ctggcgtgag gcaagcatca
      181 aacctttgtt gccgtctgtt tgtgtggatg ccgcatgcag tggggcgccc ggcacgttgg
      241 aatatcgcgg tgtaaatact gaaacaggcg aagagatctt tcattcgggt ccatatgccg
      301 atgggacgaa taatgttggg gagtttttgg cgattgtcca tgcattgaca tggcaggaaa
      361 aacataatat ccatatccca atctattcag actcggaaaa tgcaatttca tgggttcata
      421 ccggtaaatg ccgtacaaag ttagcacata cctcaaagaa ttctatttta tttgccatca
      481 tcaaaagtgc ggagaactgg ctggcagaaa atgaattgcc agaaggcaag atcattaagt
      541 gggataccaa tgtatggggc gaaaacccgg cagattttgg tcgaaagtga aaagtgagaa
      601 tccattcaat ggtgagaatc aatttcagtt cccgtatgag gcgggggttt aagtcatgag
      661 attttttggt ttatcttggt cgctgggtcg cctgcgcgga gtggatatcc gtttccattt
      721 ttcgatgctg tttagcattc cgattgctta ttatcttttt gacccgattg acctgcgtgg
      781 tgtggttgaa gctttattgt ggttggttgg ctttacgata tttattcttt tgcatgaagt
      841 ggggcatgcg tttgccgcgc aaatggtagg cgtggaagtg aaaagcattg tggtctggct
      901 cttgggcggg ttgacaaacc ttgcgtataa accagagaag ccagcacata atttctttgt
      961 ttatgcagcc ggacctttga tgaatatgct gttggcgttt ttgtgtgtgg cggcatatat
     1021 tcttacagcc atgctttttt tgccgttttc gaaagacccg caaacttata tttggataca
     1081 aacttttcag aaccttttct tttcgcttgc aattgtcaat ttgattttgg cattgttcaa
     1141 ccttatcccg gtttacccgt tggatggcgg taatattttt cacgctgcca tggaatggtt
     1201 atttggtaaa accaatgcag accgcatcac tcttgtagtg ggtattccat ttttgctggt
     1261 attgattgtc ttcggcgtta tctctcgtga ttatgtcttg cttgcttttt gtgtgctgat
     1321 cgcactttcg atcagtacct tgaatcattc cttgctcagg aatatcaatc ttggaattac
     1381 ctatctcttt aagcgagctg gctattatta tttgaagggt gattacgaac gtgccgcgca
     1441 actttataca gctgagatcg aaaaacagcc tgacgatatt aataattatt tggcaagagc
     1501 tgggtgttat cttgccatgg ctcagcgtga gcgagcactt gccgatgtag accgtgtgct
     1561 caaattacat ccgaatcatt ttctcgcact tgaattgcgt ggtgagattc tgatgctcaa
     1621 taaagattac gatgctgcgt ttgcacattt caagcaggtg caacacataa atgcgaattg
     1681 ggcagttcct tactttgacc ttgcttcact ctatatggaa cgtggtgagt tacagcctgc
     1741 aattgagaac tttaataagg ctgttgtctt gcagccgcgc atgcctttgt tctatatcat
     1801 ccgttcactg gcgcatttta aggcgggcga cctcgcgtct gcgcatcatg accaagatgt
     1861 ggcggtggga ctttcgccag aagaagcctt agtgatggtg gatgtcaact tgattgtgta
     1921 tgaagaccat tgggattggg cgaaggatta ctatgagcgt atcttgacaa aaaatccgcg
     1981 gaatgccttt gcgcttcagg gcttcgccga agcttgcctc gtcaaccaaa aatgggatga
     2041 ggctgtgacc tttttctcgc gtgcaataga gcagaatcca cgagaggcac ggctttatgt
     2101 ggggcgggga aaagcatatg tagaattaga aaaactccaa gaggcaaaag tagattttga
     2161 aaaagctgtt gcgtcgtcgg aaaaattgca tttgaaaaga caggcagaaa atttcttaag
     2221 aaccttggac atataggaaa aggatgtgtt atgaaaacag ctttacttgt gattgatata
     2281 caaaaagatt atttccccgg cgggaaattc ccgctcgtag aaccgcttgc ggctgcagaa
     2341 aaagcatatg gactgctaca atgttttcgc gaaaacaacg aaaagcatgt tcatattcaa
     2401 catatttcgc tcaagcccga tgcgacattc tttgtccgtg gaacagaagg ctcagatatt
     2461 catgatgcgg tagcgcattt tgaaggcgaa cctatcgtct acaaacatta ccccaattcg
     2521 ttccgggaga cgactttgtt cgatatgctt aagggatggg gaatagagcg tgtagtcatc
     2581 acagggatga tgacccatat gtgcgtggat gcaactgtcc gtgcggcggc tgattttggg
     2641 tttcaggttt gggtggcaga agatgcttgt gccacgcgcg atttggaaca taatggcact
     2701 gttgtccccg cggaccatgt ccatagggcg tttttggcgg cgttcaagtc gtatgggcag
     2761 gtgatgatgt cggagcaggt gatcgcgaga ttggctgcag agaaagccaa tgctaggtaa
     2821 gtagagtcaa ttatcggcta agaaagaagc gaagaagctg tcacgttttt tgtgacagct
     2881 tcttttatcc tattctttga gtaagacgat aaacgactgg taatcacaga ctttttcgaa
     2941 gccggctttg agcgctgttt tcgcagaggc ttcgttgcgc ttgaaacaat gccagccgat
     3001 ttcggttatg ccatgagaga aggcatgttc gagaaaagcg agagtgagtg ccgtagcgag
     3061 cccgcgcttg cgataatcac tgatggtttc gatgccgatc tcgcagcgac cattggcatt
     3121 gtattcagag agacaccaac ctgccaattc gccttggtag atggcacata ccccaaaact
     3181 tttagcgagg aaatcttcaa cgctggggcg ttctgaggtc atctcttctt tgagcgtctc
     3241 gaggtgtttt aggttggaat ctgcaagaag tgctgcgtct acagttttta tttcaaatcc
     3301 ttctggtaga aaatcttgcc atttgtgctt taactttttg cagacatagt attcgcgatc
     3361 tgcatggatt gtcttttttc ctgaaagcag agtatcaatt cttttctccc atgtagatga
     3421 atcatagtaa agcgcaaagc cttcatgccc tgctgcgatc tcgcgtggaa aaatgacgtt
     3481 tgtaaataat ttttgaattc cgagattgaa gctatctctt tcggagtcac cggcgagaat
     3541 atatctgtga cctgcttggg caaacaaggc tttggggtgt tcgtgattgt ccacaaagat
     3601 ctgggctggt gtttgtttgg cgagaattga acgaccgatg aggtgaatat ccatgttgag
     3661 cagcagcggg cggatgagtt cgtaggcgtt gggagggaat gcggtcaaag ttgagtttcc
     3721 aatcttttgt ctttttagtt aatctgaaat gaaccgtgtg gcggtggtat tttctgttcg
     3781 ttgtttgaag agcttcaaac caataatgat cgcgctggta accgactgaa agatagtgga
     3841 aaccataatc gtgaggtctt tttgcagaat tccatagatc agccagatta acccacctga
     3901 gagagtcagc aaccatgtgc ccagcgaaag cccagaaagg ttttcttctt tgtacgctac
     3961 tcgtatttgt gggacgttcg aaatcaatgc gctgatcgaa agcattagcc ccagacctgt
     4021 ttttccaaaa aaagttactg ctgcaaagag gacgataaac caaagcgggg tcacgcgaaa
     4081 ttcacgcgcg cttcgcccaa ggcgcaaggc agccactgtg ataataaaaa agaagctggc
     4141 tatggcgcca gatgcaagtg cgacgaaaat ttgatttgtg atgatgccgt aagtagtcca
     4201 cccgaggctg acgacacagc tcgtgccaga agtgtccact gagacgccga aagatttccg
     4261 ttcacgcaaa agccgtacca gttgtggcgc ggcgcgaaga aggtcgatga tggtgcctgc
     4321 aaatccaaga gttgagatga aattcatttt tgtcctaata gggaagtttt actggtgata
     4381 gtgagcgcat attataattc cgcctatgca aacgaccctt gaccccgaac gacaaaaaca
     4441 agcaaaagag tatgcacgta ttcgccgccg cctttggctg gttgatacac tattgaatgc
     4501 cgcctatgcc attgtctggc ttgtattcgg ttggagcatt tctctgcgcg agtggctcac
     4561 tacgatcaca accaatgaat ggttgttagt cgcattatat gttgccatct ttggcggcat
     4621 attctctgtc tttactctgc cgcttggtta ttacagtggc tttattctcc cacatcgttt
     4681 tgaccaatcc aatcaatcgc tcaaagattg ggttatcgac cgcatcaagg gacttgccat
     4741 tggtgcgccg cttggtttgt tgatgcttga attgctctat cttgcattgc gcctcactgg
     4801 gagtctctgg tggttatatg ctgctggtgg attgctactc tttaatgtca ttctttccaa
     4861 ccttgcgccg gtgctcatta tgccgttgtt caataaatat gttccgcttg gcgatgaaca
     4921 taaagacctt gaagaacgtc tgctcaatct tgcaaaacgc gccaatacca aagtaaaagg
     4981 tgtcttcaag ttcgatatgt ccaaacgtac caaagctgcc aatgccgcat tgaccggtat
     5041 gggcaatacg cgtcgtatca ttcttggcga taccctcatc aacgaattta caccggatga
     5101 gatcgagacg gttcttgcac acgagttggg gcatcacgtc cataaagata ttcccttctt
     5161 gattatctcc ggtacgcttc ttacgaccat tggtctgttc attgcctctc aaaccttaaa
     5221 ctattctgtg acctttttct cctttaattc tgtcgcagat gtggctgctt tccctgcact
     5281 ctctctaatt cttggcgcat atggcttgtt gactcaacct ctcgaaaatt atctttctcg
     5341 ctggcgtgaa aacatggcag atgattatgc cttagtgtcc acgggcaaac atgaagcctt
     5401 tgcttctgcc ttcactcgtt tggcaaacca aaaccttggc gagatcgacc ctgagaaatg
     5461 ggtggtcttc atgttctact cccatccgcc attaggcgag cggattgcga aagctagaaa
     5521 gtttacggct taaaagtata ggtcacgctt caagcgtgac ctatttattt cttgtcttcc
     5581 aatacttttc ttacaccaga tctcccatca attccatcgg cacaactgcc gcaccgacaa
     5641 tgccgggacc tgtatgcacg cccaatacag gcgagatgcg ctgtacttcc attttggcga
     5701 cattcaattt ttgtttgaat tcttcggcga gttgatctgc tttctcagcg aagcgaccgt
     5761 gcaaaaccgt aacccaaact ggagcatttc catatttctc aacgagatgg tctgcgataa
     5821 tggacatgga tttgccaatg gaacgtccct ttgcaacggt gctatatttt ccgtctgtat
     5881 ccacacggat gacaggcttg agattgagta aggctccagc catagcttgc acacgcccga
     5941 tgcgaccacc acgagcgaga tattccaatg tatcgagagt gtagatcact tcggtcttct
     6001 cgcggatttt ggtcaggcgc tcctgaactt tgtccattgc ccaacccgct ttgtctgcca
     6061 atgccgctgc catcacttga aagcgctctc caccagagag ggtgagcgta tcccaaaagt
     6121 tgacgtttgc ttcgcctttc acttgttcgc caccatctcg tgccgagttg atcgtgccgc
     6181 tcagtccaga agagatatgc accgatagaa tatttttatc tatttgtgcc agtttgcggt
     6241 acaactcggc gaagataccg ctggatggct gtgctgtagt cggaatttgt gggcgcattg
     6301 cctccaaacg attgtagaac tcatcggcag aaatatctgc tgagtttact tcgccttcag
     6361 ggaattggat aaacaggggg gcttgtacaa ttcctaactg tttcaattcc tcattggaca
     6421 tatctgctgc gcagtctgtt acgattttca tggtttcctc ttttggttag attgattcgt
     6481 tgtttgaata gtatttttgt ttttcaacat gctagcgacc agcatggtgg tgctgtcacg
     6541 tgcgaccttt tcccattttg cacctttggg gtccatgagg ctttgtagca acaatcccat
     6601 tgcggttgaa atgatcatgc gtgaggctaa gtcagggtcc acttcgacga atgagccttc
     6661 gtccacgcct tttttgatga ggtcagtgaa gtatttgtga tagcggcgat aaggcttcac
     6721 gcttgcttgc catatctttt catcgcggct ggcttgtaac cagaactcaa ggaacatggg
     6781 caagccttcg tttgccgttg cgaagatata ggggaatgct tctgtgatct gcatgaaggt
     6841 ctcagggaca gttttgtcct tcgaggcttc gatggcgtta tcgatggttt ccagccagcc
     6901 atcaagcagg gcaaggaaaa ggtcttgctt ggtcttgaag tgatgataga acgcgccttt
     6961 gctaatgcca gcatccgcgc agatatcgtc tacactggcg gtgttgtagc ctttggctga
     7021 aaacagtttg acggctgaga aaatgatctt cgaacgggtt tcttcactgc gttgttgcat
     7081 cttgtccttg agacgagtca ggtgctgaaa aaggaataaa accgactggt cggtttgttt
     7141 ttacctctga aactttgcac tgtcaagatg agggaactca tgtttaaaag aaaagcggac
     7201 agagcccaaa ggttctgtcc gctatgttga gttgaacgat tataagaata ctggtgccag
     7261 taaatcgtac aacatgccca gaacaatagc gcctgttacc aaggttccaa ggacgatgcc
     7321 gatcacacgc caacgggcga tggtcattgc acctgccaag gcgccgatag acgtccccgc
     7381 gccggtgatg aggaaggcga gagccgcgcc tgcactcatg ccagagtcca tgaaggcttt
     7441 gacgagtggc atagaggctt cggtgttgag gtagaaggga atgccaagga tggcagccaa
     7501 aggaacgcta tatgcgttgc cagaaccaaa cgctgtctta atccattctt gggggatcaa
     7561 gccgttgagg aaatacccaa ggaaggcgaa gccaaggaac atagcaagca gtttccatga
     7621 ggtggtgaag gtttcgtctg caaactgttt gaaggttacg acctttttgg ttggaatggg
     7681 ttcggatgcg atcacaggca atgacaatga aataccagct gtttgtgtcg caaattgtgc
     7741 ctgtccttgt aagagggctt ggggctggaa aacggtttga gaaactggag ccggcttgag
     7801 gtctgctcga ggcgctttat ctgatgcgaa acgtccctgg ttggcgagcc atccttttga
     7861 ttcggcgaag gctgcaatgg cgccgccagt caaacccaag atgatggatg caacgaagaa
     7921 tgtgatagcg aagggccagc caaacaaacc ggcactataa aaaagttctt gcggagaggt
     7981 gagaggggag gcgaccatga atgcaacaat gggcgcccat ggaatggtgg atgccatcat
     8041 gccaagcacg acggcggttg taccgcatga gcagaaaggc gtgccaattg ctaacgctgt
     8101 agacatggca atgctgttac tttggttgcg tttgagaaat gcagcgacac ggtcttggtc
     8161 aacatatagt ttgagaatga cgctgatgag aacgctcaag aggagcaggg gccataagcc
     8221 tgaaaaagtt tcccaaactt tgctgagtgc gaattgaagg acttcaagaa gattcattga
     8281 tcatctccaa aataaagatg tgagtttaga aggatattca tggttttatc tttcatatag
     8341 aagtttatct ataagtaaaa gcaaaaaaat ttaccaataa tgtctctctg cctgccaaat
     8401 ggcaaacaga gatcgagctt agcagcaacc gctggggcag ttgccgcctt cacaacattc
     8461 gctttcgcaa caatcgttgg tgacgacagg taattcgttc atggaatact cctttcgtat
     8521 agaagtttgt ctatggaact gattgcaaaa aacgggatca tgtcacggcc gtggacatat
     8581 cccggttatg ccttgagggt tttcaacagg ttttcagtag taacggattc gggcgcaaac
     8641 ttgaccatga tctgcccgca acatactgca atatgttcct ggttgagggt atagaaagtt
     8701 tgtttgcctt cttcgcgcac attgaccaaa ttggcatcgc gcaatatggc gaggtgatgc
     8761 gaaatggtgg gctgtgaaaa gccaattttc tcgacgatct cgctgaccga gagttcgcaa
     8821 caacaacact cgctcatgat tttctggcgg gtttcgtctg caatggcttt ggcaaacagg
     8881 acagggtcaa atttcatgcg gttattatat agatgtttgt ctatttgtca agataaaaaa
     8941 aactccgagt ctttaagacc tcggagtttt gagagctggt tatttacccg ttaattcgcc
     9001 gagtctatca atgtattctt ccagcacggc aaatgcatct tcgatgggtt gggtggaagt
     9061 catgtcaatg ccggcggttt tgtaaacctc cataggggac atggaggaac ccgccttgag
     9121 aaagttgata tggtcttgcg ccgcgccggg gactccgtct agcacgcgtt tagcgatggc
     9181 gtttgcagcg gagattccga tagcgtattg gaaggagtaa taatcaatat aaagatgcgt
     9241 ggtgaatgtt ccccaagtga taccgacccg ttcccggtcg aggtgcatct ctccgccaaa
     9301 gccttcgctg aataggtctg ccatgaggtt gttcatataa tcggctgtga gagactgtcc
     9361 cttttcggcg cgttgatgcg tttctaattc gaaacgagca agagtgggca tgatgaaaaa
     9421 gtagcggtgg aagttatcca tggcttcttc gagtagagcg atctggaagt atttatcttt
     9481 tttcgtcttg agcaggtggg cgcgggtcat tgcctgatta aagttcgagg cggtctctgc
     9541 aatgatggag ggatagagat aatacatcat cggctgcgca cggctggcat accaagcatg
     9601 aatggagtga cccaattcat gcgagagcgt gctcatcgag ccgacatcat ctgtatagct
     9661 catcatgatg aaaggatgtg tatcacttgg gacgcgagta gagaatgctc cttcacgttt
     9721 gcccgcattg ggagaccagt ccacccaacg gtcttcgagg caacctttgc gcatggtttc
     9781 aacatattca tcgcccatgg gcgcgagacc ttcacagatc cagtccacag cggtttcgaa
     9841 tggaactttg tgtttctttg tagtcaacgg cgcccagaca tcataagggt gaaggacttt
     9901 tacacctaat gccttgcggc gaatgcgcca atacttgtgc cacatgggta gttttttctt
     9961 aaaaatatcc aataggttat ggaacatttc gaccggaaca tcgccattga aaagtgttgc
    10021 ttccagtgaa gagttgaaat tccgtgcttt catgttgaat acgttttgtt tgatcgaagc
    10081 agccagattc cctgccagag tattcttgaa ttcaagatat ttatcgttgt aattttccca
    10141 agaagtttgg cgcactttac ggtctgctga ctcaatgaga gcacttcttg tgctttgagt
    10201 caattcgagc tttctgcctt tgctgtcttt ggcgggcttg aatttgaaat cggcatttgc
    10261 caacattcca gccgtattgc ggactgcccc gaacgggtcg cgtaccatgc caagcaattc
    10321 ttccacttcg gcattacgca catgcgcttg tttgcggaat aagtcattga ggaagtgctc
    10381 gtacagtttc atgcgcgagt cttcgttcac ccattgacgt aatttcgcct cacccatgga
    10441 cagcaattcc ggctccacaa atgatgtcgc tgccccaact tgtgccagca tggacatggc
    10501 tttgctgctc atcgcagcgc cttgctcgtc gtttgcgtcc acggcgctcg ccatggtggc
    10561 atacacgcgc acctttgcag tgcgcttttc gagcttatct atcgcttgaa acgcttcaag
    10621 aaacatttcg atgttttcac caagatgccc ttggtatttt ttgatttttg gaaggtcagc
    10681 tagaatattt tgcaattcag tatcgaaggc ttcaggcgtt ttaaagacac tttctgcatt
    10741 ccaagtttgg tttttcttta cttttgagcg gggtagtggt gtagtcatga tacagttcct
    10801 ttcaatgtgc gctgaaagat aaataaaaat gtactccgta tttttagcta tgttgccaaa
    10861 atatcggagt acattttgtc atcttcctga aacgatagac ttactgcttg gttagcgtgg
    10921 ttggaacact ttgcgatgtg ggagcgcccg tcccaccggc gctgctgctg aattggatgt
    10981 tcacagcgcc caaatctgcg tcggtgatcg tatagttatt tgtacactgc acggttgcat
    11041 tgggtgctag tgtggtattg gctgcaccgc aatttaatgt ggctggattc atcaatgggt
    11101 cattaatgaa gaattgcgta ggacctagat ctccactgcc agtgttcttg atcgtgtagg
    11161 tgaaggtgat ggtctgccca gcggtgttgt atgtggtggc actcggtgtt actgtaaggc
    11221 tgagttgagc agttcctttt gtgagggtgg tggcaactga agttgtagtt ggtacgttcg
    11281 ttccagatgc tgttgtactg aattgaaggt ttgccactgc caggtcgttt tgggtggttt
    11341 tataggcgtt ggaacaggta agggtcgcgc cgggggcgat ggtcgaattt tccaatccgc
    11401 acttgaatgc cgtagggctc atcagcgcat cgttgatcat gaattgggtc ggtccaagag
    11461 tcgtagaccc gttattcttg atgacatagt taaaggtgat gatctggtct acctgtgtat
    11521 aaatagtcgt attcggggtg accgtcagag acattcccgt ggttgttgga gaaaccgtgg
    11581 caaccgcatt ggaaagtgga atatttaaac ccttggtata aagtggaact ttaacctgct
    11641 tgccgacgat catattattg ctgttagaga gttgtgtgag cccagggtct gcgccatatt
    11701 tatcggcgat ggacgtccac gtatcgccag attgaacagt atggagctga acacaaagct
    11761 ctggcggtgc atggacggcg cgattcccga cagtgccaac attgggaaca gtgacagtag
    11821 tgccgatctt caagtttttc gggtccactt gcgggttcgc gctaacggtc tgattgggat
    11881 ctgccgaata acagcgggcg atttgccata gccattctcc ctcgcggacg gtgtgttgca
    11941 cggttgttcc cgaagcagca gcgggcgcgg aacctttggt tagtgaaaga gccgcgggtt
    12001 gtgaggtcac accgccacct gaaacggttg cagagtgaga aatagtcgtc gcgttcatat
    12061 ctgcagctgt aacggtgtag gtggcgttgc agttcaaggt cgcaccgggg gcgatggttg
    12121 catctgcatt gccgcagttg aacggctgat tattgctaat acccgtatcg gcaatggtga
    12181 attgtgccgg acctaattga acagttccgc tattcttaat ggtataggta aatgtaattt
    12241 gctgatttgc tgcaccataa gttgtggtgc ttgccgtttt cgtaagcgtg agtgctttgg
    12301 gcgaaacggt tggcactgtg gttgtcactg tgtttgaatt aactgaatac gcattggcag
    12361 tgactacatt tgcgattgaa cctttatcga ggtctgcttg ggtaataaga taatctccag
    12421 tgcattcaag cacttcgccg gggtcgaagc ggtcattgag attattgata gtgttgagcg
    12481 ccgggcaagt ggctgtaatg cctgtgatcg taacatttgc cggagtgccg tcaggcaggt
    12541 catttttgac cattttgacg agatacttaa agcgaacagt ttgcccaacg gtattgtaga
    12601 ctgctgctgt atcgtattga gttgtaagct caatcgtgac taaatctgta aggctttgag
    12661 ttgcctgaac ttcagacaat gcatttgcgg cgggttctgt tgagctgcca aagagcgtgc
    12721 aagatgccag cagtaaactg gcaatgctca acagggcaag cgagcggacg atggttttca
    12781 tgaccacttc ctttcgtttt gtaaaattgt acgaccactt ttattatatc ctctatacat
    12841 ctggattaag aatcatcccg cagggttcgt aagtctactc tgcgggatga taatgcgagg
    12901 attaaagcaa caaattaact gggaccggat ataggtggat ttgttttgga tgtaccgtcc
    12961 ttaacaagga tccagtaggg cttgttcccg aatcgtgcgg gatttgtctt ggtcacatct
    13021 gcccaaactt tttgcccact gacaacgact gtcttgcgcg cataaaaata tttctcagtg
    13081 atattcgcgc cagccagaaa ttctccatta ggcgctctat gaataggcgt gttgacggag
    13141 atggtaaacg ctgcaaattc agaaggcggc agcaacaaag tccaacgtgg gtcgaaagag
    13201 ttggcgggga tctcgtccca gaaaaagcct ttgggattcg cggtttcacg gtctagaacg
    13261 atcttctccc agagcttttc atcggcaagc tgttcacgag caatttgttt caagcttttg
    13321 tctgtaggct gaactttata tacaataaaa tacccggctg cttttggagt tgctattttc
    13381 ttaacttgtc cgtttgccat ttttctctcc tttgtttttg aaccacaact atagaatacc
    13441 cgaatagaag tggatgtcaa tagctttatt tgttgaagcc aagtcccttt gcgaggattt
    13501 tatcaattgt acgtttgggt aagattgaca tgacccaatt tgaaaatgga ttggggtcta
    13561 ctgcatagcg cacgcgagga t
//
LOCUS       DAOQNJ010000008        13491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_1917_length_13491_cov_8.564082, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000008 DAOQNJ010000000
VERSION     DAOQNJ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13491
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1917_length_13491_cov_8.564082"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLG52_00540"
     CDS             <1..566
                     /locus_tag="PLG52_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB39953.1"
                     /translation="ERLGSDIQRISDATEKMHRLLSELLDLSRVGRVASEKQNVPFEA
                     IVTEALKRVEGQIKEKQVEVQVRSGFPVVHVDKERLIEVIQNLVDNAVKFMGKQPSPH
                     IEIGFNMQDGNPIFFVRDNGIGIKKEFQKKIFGLFDKLNPATDGTGVGLALVKRIIEV
                     HGGSIWVESQEGMGATFYFSLEKNNEN"
     gene            649..1212
                     /locus_tag="PLG52_00545"
     CDS             649..1212
                     /locus_tag="PLG52_00545"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNB39954.1"
                     /translation="MSLKHTILGFLSYTSLSGYDLKKAFDRSVQHFWPANQSQIYRTL
                     AELEEEGFVTKEVIEREERLDIKLYHITTNGRNELHQWLIHPLPERDDREPFLIQVYF
                     SGQLKDTEVLHLFRQKLRETEERLAVYESVYEMIQNTPKRIHDDRAIFFSVLTLEAGY
                     INNKSIAEWLRSAIERIENKNYEINIG"
     gene            1214..2053
                     /locus_tag="PLG52_00550"
     CDS             1214..2053
                     /locus_tag="PLG52_00550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNB39955.1"
                     /translation="MTKTIRNIIIVSLFTVGGGWLGIWLNNVTGNTMPPMQSLGALVW
                     LVSPALAGFALRTFGGDGWKDSGFGLHLLSSWKWYLLAILIYPLASLLTFGLGTLFGI
                     TSAEGFSTQGFSAYLSAVGAIFIGSLMKNFFEEFAWRSYLTPRLEAAGAHPIVNHVLV
                     GILWMSWHLPYYYYYLDRTVLKSSITTSIPVFIALGYLVMIPTAILFGELRLISKSTW
                     VVFALHQVINAVSMPLLINGFIEVKGPLAPVFTPTNEGLFVSLLFGVTGWLLMKKRLQ
                     TQA"
     gene            2160..3737
                     /locus_tag="PLG52_00555"
     CDS             2160..3737
                     /locus_tag="PLG52_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB39956.1"
                     /translation="MEREKLAFFNPATNTKFGEVVVNTPEEVQQAVREMRQAFPEWSR
                     KSIQERADILHKLQHVLIEQRDEISNVLNMDCGKSRQDGLLELFVAIDMLVQYRHNAK
                     KWLRRQRVSSGLYIFKRCYIEHRPHGVTAIISPWNYPFTLSLPPMVSALLAGNTVVLK
                     ASEVTAATGVLIEKLIKSVPELAPFVRVVHGDGRVGAALVASKPDYIFMTGSTATGKK
                     IMQTAAETLTPVALELGGKDAMIVLEDAELEAAARWGAWGAFFNTGQTCMSVERVYVV
                     ESKYDEFVNLAVQQAQELKSGYSTELNSPFFMGPVTDQRQVKIIDGHLQDALDKGAKI
                     LTGGKRDGMFYDPIVLVNVDHSMKIMRDETFGPVMPIVKVKNEEEAIHLANDNAYGLG
                     ASIWSNDISHAKRVADRVEAGSIIINDSIAQFGVPMLPFGGTKDSGFGRTHGKEGLLQ
                     FTRPYSFAIGGAPVKMDVATVLREMGHYELATMIMGVLFGTTLKQKVETFLQFFRRGK
                     KQAPPAGETSSNTAAAD"
     gene            complement(3805..5535)
                     /locus_tag="PLG52_00560"
     CDS             complement(3805..5535)
                     /locus_tag="PLG52_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNB39957.1"
                     /translation="MTDYKSQIWRINTRTQELKREPVPETWQRLGGRGLIARIMVDEV
                     DATCDPLGPGNKLIFTPGLLVGHILSSTDRISVGGKSPLTGGIKEANAGGRTGYHMAF
                     MGIHALILEDMPEKDGYWVLHLSLNDGAKWERADELVGLGVYETAPKLIEKYGDKVAI
                     SLIGPGGEMRMKSAGIQNLDKDKIPSRIAARGGLGAVMGSKGLKAIVFDHIGGQKPPI
                     ADVEAFKVAQKDYTQSVMNHPQSVTYREYGTQAMPQMTQNFAALPTHNFSRGTFDDVE
                     KISGETMREFILNRGKPSDPSHACMAGCTIKCSNVFGGEDGKIIVSPLEYETVGLMGT
                     NLDINSLDSIGRMNWQVNDLGLDTIEVGGALGVAAEAGIMRWGSEEDGLKLIDEIRKG
                     TELGRVLGDGAVAVGKKYGVERVPAVKGQSMSAYEPRSIKGTGVTYATTPQGADHTCG
                     LTIRAQVNHLDPTQQKDASLNAQLNMAGYDTIGACIFAGFGYAATPDGVVKRLLKARY
                     GWEDVPDNVLQALGKETIKLEREFNKRAGFTKEDDRLPKWMMEEALPENGSVFDVSED
                     VLDHIFDGIE"
     gene            complement(5538..6899)
                     /locus_tag="PLG52_00565"
     CDS             complement(5538..6899)
                     /locus_tag="PLG52_00565"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNB39958.1"
                     /translation="MNTTLDRSKLEKLLKQEEELFHKTHPKSYELYQRARKSLHGGVP
                     MLWMIRWAGSFPVFVKEAKGAHFTDVDGNNYIDFCLGDTGAMTGHAPEATVKAITEQI
                     QKGITLMLPYEDVIWVGEELQRRFKLPYWQFALTATDANRFALRMARLITGRPKVLVF
                     NYCYHGSVDETFITLDEEGTPISRPNNMGPQIDPRETSKVIEFNDIAALETALSARDV
                     AVVLAEPAMTNIGIIHPDPGYHEALRALTRKYGTYLIIDETHTICAGPGGYTASYGLE
                     PDFITLGKPLAGGVPAAIYGFTEEVSKAFSAKLAMDDADVGGIGGTLAGNALSIAAMK
                     ATLQNVLTDEFYAKATTLQEQFTAGVEGVIAEFKLPWIVKRLGNRSEYWFRPTPPKNG
                     GEAHAAVDPELDRYMHLYALNRGILMTPFHNMALISPETTQDDVDHHTKVFREAVKSL
                     FGE"
     gene            7013..8113
                     /locus_tag="PLG52_00570"
     CDS             7013..8113
                     /locus_tag="PLG52_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB39959.1"
                     /translation="MTETPSANLLDSLDLNKRFKTSAYFTICFNSLPSSLHLVLSSDK
                     KQDAVDAILAEMELEKFNFTTVFKNWSTEDFQNSQPSDDYVFEYVFKSEAGRMLIHFT
                     ARDLSYITVEFLYDHDQKGSEEWVLAMNHKLRSKFGAPKKPQFKVLMHQDGMFGMEEV
                     STNDFKSIDIAELYNDDFVEVDKAISTFMGKQESGIILLHGEPGTGKTTYIKHLICKF
                     IDREFIFIQNDFVSDLLKPNFIAFLLQNKNAVLIIEDAEKVVVPREHSSEASVVSTIL
                     QLTDGLFSDFLNIKIICTFNTDIERIDKALLRKGRMIAKYNFSALSPAKTAALAQKLG
                     HEHVEGSLTLADIFGYDKLDFKEATKRKIGFV"
     gene            complement(8110..9390)
                     /locus_tag="PLG52_00575"
     CDS             complement(8110..9390)
                     /locus_tag="PLG52_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB39960.1"
                     /translation="MTNQLYQDNFSHVTPAWSRIFNFVADRAEGSYIYTDDGKKLLDF
                     TSGIGVTNTGHCHPKVVEAIREQAGLFLHAQANIVIHKPMLQLIEELRKVVPPSIDSF
                     YFANSGAEALENAVKIAKAVTGRQNIIVFNGSFHGRTHATMALTTSKTIYRTGFAPLP
                     AGIYVAPFPYAFNLKMTEEQASQYALEQLEYLLASQTAPKETAAILIESVLGEGGYVV
                     PPASFMKGLREICDKHGIMLIFDEVQSGFGRTGKWFALEHFGVVPDIITAAKGIASGM
                     PLSGVFSRTEIMKKVDTGSIGGTYGGNAVACAAGVATIRAMREEKMLENATERGIQLM
                     TGLHKLQEEYPQIGDVRGKGLMVGTEFIVDGSQAKAKPLVKEIIHKAEEKGLLLLSCG
                     TYDNILRWIPPLNVSSEQINAGLKIFGEAVKGSV"
     gene            complement(9478..11385)
                     /locus_tag="PLG52_00580"
     CDS             complement(9478..11385)
                     /locus_tag="PLG52_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNB39961.1"
                     /translation="MPNGYNGKILHVDLTKGSLTVEEPNEAFYRKYLGGSAMGMHYIL
                     RDMPKGADPLGPENVLTLFTGVTTGAAISGQSRLNANAKSPISGGIGDSQSGGFFPAE
                     LKFAGFDGIVVKGKSPKPVYLCIIDGKFELRDASHLLGKLSGEVDDIIHKEVDPKAEI
                     LQHGIGAENGVLYSSLVSMSNRHNGRTGMGLVMASKNLKAVVVRGTGKIKLANQKALT
                     ELNRIGPKAIPENGDMDGLAKFGTAVVVLFNNTIGTLPTRNYNEGQFEGCEPISGEKM
                     AETVLKERDTCYACVVKCKRVVEIKDGPYKVDPRYGGPEYETLGTFGSYCGVDDLAAV
                     SLANQICNEYAVDTIAAGATIAFAMECYEKGIITKEQTAGLDLKFGNAEAMIAALNML
                     VKGEGEFGKTLGLGSERAAAKWGNGADECLITVKGAEAPAHMPQAKRSLALIYAVNPF
                     GADHQSSEHDPYYEEGVGDFNLDRLKQIGLGSPQPAYSLTEEKVRFAYETEVFYSMLD
                     SAELCQFVYGPTWTVYDGEQTAQMISSVTGWDITLNELMEVGRRRLDLFRVFNAREGL
                     ARNADKLPKKFFKELKGTGPTAGFALTHEEVESALDTYYKMAGWTNNGIPTRETLKKH
                     DVEWAAEYLPA"
     gene            complement(11604..12803)
                     /locus_tag="PLG52_00585"
     CDS             complement(11604..12803)
                     /locus_tag="PLG52_00585"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNB39962.1"
                     /translation="MKVLLVGVGGVGEAIAAIAKPREWMELMVLADYNVKRAEEVQKK
                     LGDPKRFPVEFIDASNQKNIEDLAKKHQVDLIMNSVDPIFNQQIFDAAYNAGVKYMDM
                     AMTLSEPHPTDPFNKPGVKLGDYQFEKAKEWEKKGLLALVGFGVEPGMADVFARYAAD
                     HLFDEIEEVGIRDGANITIEGYDFAPNFSIWTTIEECLNPPVIWQNGDWHTTAPFSEP
                     EVFDFPEIGPVEVVNVEHEEVLLVPRWVKAKRVTFKYGLGNEFIEVLKTLHKLGLDNK
                     NKINVKGVQVAPRDVVAACLPDPAHLGDKMKGKTCAGTWVKGTKDGKKKEVYLYQVAD
                     NEECMKTWGCQAVVAQTAFTTVIAMDLLEHGAWKGTGVLGPEAFEPTVFMEKMADYGF
                     PYGIKNM"
     gene            complement(13128..>13491)
                     /locus_tag="PLG52_00590"
     CDS             complement(13128..>13491)
                     /locus_tag="PLG52_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB39963.1"
                     /translation="AEQLNVSPGMIRQRYNRLVDLGYLKVVAVTNPLMMGKRTMSMIG
                     IRTDGRKMMEVANKIAKFDEVVYIVATSGRYDLMIEVFCADHEDLFNFMAEKLAKVDG
                     VRETESFIYLKIAKEIYF"
BASE COUNT         3549 a         3459 c         2977 g         3506 t
ORIGIN      
        1 ttgagaggtt aggttcagat attcaacgca tatcggatgc gacagaaaaa atgcaccgtt
       61 tgctcagtga attacttgac ctttcaagag tagggcgcgt tgcgagtgaa aaacaaaatg
      121 tcccctttga ggcgatcgta acagaagccc taaagcgtgt agaaggtcaa attaaagaga
      181 agcaggtcga ggtccaagta agaagcggat ttccagtcgt tcatgttgat aaggaaaggt
      241 taatcgaggt catccaaaac cttgtagata atgccgttaa attcatgggc aagcaacctt
      301 ccccacatat tgaaattggt ttcaatatgc aagatggaaa cccgattttt tttgtgcgtg
      361 acaatggcat aggtattaag aaagagtttc aaaagaaaat tttcgggcta tttgacaagc
      421 tcaaccccgc tacagatgga actggcgttg gtttagcatt ggtcaaacga attattgagg
      481 ttcatggcgg cagtatatgg gtggaatcgc aggaaggcat gggggcaaca ttttatttct
      541 cacttgaaaa aaataacgaa aactagtgta ggcttttcct tgacaggagt cttggaataa
      601 tatatagtac taatcatata atacaagtac tatataaact acacgcatat gtcattaaaa
      661 cacacaatcc tcggattcct ctcctatact tccctttctg ggtacgacct caagaaagcc
      721 ttcgaccgtt ctgttcagca cttctggccc gcaaaccaaa gtcagattta ccgcacactt
      781 gcagagctcg aagaagaggg ttttgtaacc aaagaagtca tcgaacgaga agagcggctg
      841 gatattaagc tgtatcacat cacgacgaat gggcgtaacg aattgcatca atggctcatt
      901 caccctcttc ctgagcgtga tgaccgtgaa ccttttctaa tccaagtcta tttcagtggt
      961 cagctcaaag acacagaagt ccttcatctt tttcggcaaa agctaaggga aaccgaagag
     1021 cgcctggctg tttacgaaag tgtctatgaa atgatccaaa acactccgaa acgcatccat
     1081 gatgaccgcg ccatcttttt cagcgtactt acgcttgaag ccggttatat caacaacaag
     1141 tcaattgccg aatggctacg ttctgcgatt gagcgtatcg agaacaaaaa ctacgaaatt
     1201 aatataggtt aacatgacaa aaactattcg caacattatt attgtttcgc tcttcaccgt
     1261 aggtggaggt tggttaggca tctggcttaa taacgtcact ggcaatacca tgccacccat
     1321 gcaaagctta ggggctcttg tttggctagt aagcccagcc ctggcggggt tcgctctgcg
     1381 taccttcggt ggagatggct ggaaggattc tggttttgga cttcacctcc tctcaagctg
     1441 gaagtggtac ctgttagcga ttctgattta tccgctcgcc tctctcttga cctttggctt
     1501 gggaaccctc tttggcatca cttccgctga aggcttttcc acccaaggct ttagtgcata
     1561 tctttctgcg gtcggggcaa tatttatagg ctctctgatg aaaaatttct ttgaggagtt
     1621 tgcctggcgc agttacttaa ctccgcgcct tgaagccgca ggtgcgcacc caatagtcaa
     1681 ccatgtcctt gtgggcatct tatggatgag ttggcacctg ccctactact attactacct
     1741 agaccgcaca gtgttaaaaa gttccatcac tacaagcata cctgttttta tcgcactggg
     1801 ctatttggta atgataccca ctgctatatt attcggtgag cttcgtctca ttagcaaatc
     1861 tacatgggtt gtttttgcgc tacaccaagt cattaatgcg gtcagtatgc ctttgcttat
     1921 taatggattt atcgaggtga aaggtccgct cgcgccggtc tttacgccta caaatgaagg
     1981 gctctttgtt tcgctcttat tcggcgtcac aggctggcta ctgatgaaaa aaagattgca
     2041 aactcaggca taataaagag gagaaaacag actcttttct cactttttgg agccaatttc
     2101 cataaacaac tggttataat tggaaaagaa aaacaatttc aaatgccaag gaacctttca
     2161 tggaacgcga aaagctagca tttttcaatc ctgccaccaa cacaaagttt ggtgaggttg
     2221 tcgtaaacac tcctgaagaa gtgcaacaag ccgttcgcga aatgcggcag gcattcccag
     2281 aatggagccg taaaagcatc caagaacggg cggacattct tcacaagttg caacatgtgc
     2341 taatcgagca acgtgacgaa atcagcaatg tgctcaacat ggactgtggc aaaagccgtc
     2401 aggacgggtt gcttgagtta ttcgttgcta tagacatgct tgtccaatat cgccacaatg
     2461 ctaaaaaatg gctccgccgt caacgggttt cctctgggct ttacattttc aaacgctgtt
     2521 acattgaaca tcgcccccac ggtgtgaccg ccattatctc accatggaac tacccattta
     2581 cattatctct accacccatg gtctcagcct tacttgctgg gaatactgta gttctaaaag
     2641 cctctgaagt gactgctgct acaggcgtat tgatcgaaaa gctcattaag agcgtaccag
     2701 agttagcgcc atttgtacgt gttgttcatg gtgacggcag agtgggtgca gccttggttg
     2761 ccagcaagcc agactacatt tttatgactg gctccacagc caccggcaag aagatcatgc
     2821 aaaccgcagc agagacactt acccccgtag ccttggaact tggcgggaaa gacgccatga
     2881 tcgtgcttga agatgcagag ctagaagccg ctgctcgttg gggagcctgg ggagccttct
     2941 tcaataccgg tcagacgtgc atgtctgtag aacgtgtcta tgtggtcgag tcaaaatatg
     3001 acgagtttgt aaatctggca gtgcagcagg cacaagagct aaagagcggt tattccaccg
     3061 aactcaatag cccattcttt atgggacccg tcactgacca gcgccaagtt aaaatcattg
     3121 atggacattt gcaagatgcg ttggataaag gcgccaaaat tcttacaggc ggcaaacgtg
     3181 acgggatgtt ctatgacccg attgtgcttg tcaatgtaga ccacagcatg aagattatgc
     3241 gcgatgaaac atttggtccg gttatgccca tagttaaagt gaagaacgaa gaagaagcca
     3301 tacacctcgc aaatgacaat gcttatggtt taggcgcttc catttggagc aacgatatca
     3361 gccatgccaa gcgagtcgcg gatcgggttg aggcgggatc aatcatcatc aatgatagta
     3421 tcgctcaatt tggcgtccct atgctgcctt ttggcggcac aaaggactca ggttttggtc
     3481 gcacacacgg caaagaaggc ttattacaat ttactcgtcc atatagcttt gctattggcg
     3541 gtgcgcccgt caaaatggat gttgcaaccg ttttgcgcga aatgggacac tatgagttag
     3601 caaccatgat tatgggagtt ctatttggca ccaccctcaa acaaaaagta gagacctttt
     3661 tacaattctt ccgtaggggc aaaaaacaag ccccgccagc cggcgagacc agttccaata
     3721 cagctgcagc tgattaaaaa actgccctgt aggaattcta cagggcagtt tttattctca
     3781 atcttgcaga ctacttatgt tcttctattc aattccatca aaaatatgat caagcacatc
     3841 ttcgctcaca tcaaagacag aaccattttc tggcaaggct tcttccatca tccatttggg
     3901 taaacggtca tcttctttgg taaaccctgc gcgtttgttg aactcacgct ctagtttaat
     3961 agtctccttg ccaagagctt gtaacacgtt atccggcaca tcttcccaac cataacgcgc
     4021 cttcaatagg cgtttcacta ccccatcagg agtcgccgcg taaccaaagc ctgcaaaaat
     4081 acatgcaccg atggtatcgt agcccgccat gttcaactgc gcgttgagag acgcatcctt
     4141 ttgctgagtg gggtcaaggt gattaacctg agcacggatc gttaacccac aagtatggtc
     4201 tgcgccttgc ggagtggtcg cataagtcac acccgtccct ttaatggaac gcggttcata
     4261 tgcggacata gactgacctt tgaccgcagg aacacgttca actccatatt tctttccaac
     4321 cgctactgcc ccatcgccaa ggacacgtcc aagttctgtg ccttttcgaa tttcatcaat
     4381 caatttcaag ccgtcttctt cactgcccca gcgcataatt cccgcttcgg cagcgacccc
     4441 taatgctcca cctacttcga tggtatccaa accaaggtca ttaacttgcc aattcatgcg
     4501 tccaatggag tcgagcgaat tgatatcaag gttggtgccc atcaaaccaa ccgtctcata
     4561 ttccaacggc gaaacaatga tcttgccatc ttcaccgcca aacacgtttg agcacttgat
     4621 cgtacacccc gccatacaag catgagaggg gtcggagggt ttgccacgat tcaaaataaa
     4681 ctcgcgcatg gtttcgccgc taatcttttc tacatcgtca aaagtgccac gtgagaaatt
     4741 atgagttggc aacgctgcaa aattctgggt catctgcggc atggcttgcg taccgtactc
     4801 acggtatgtc acagactgcg gatggttcat gaccgattgg gtgtagtctt tctgagcaac
     4861 cttgaatgct tctacatcag caattggagg tttttgtccg cctatgtggt caaacacaat
     4921 agctttcaaa cccttcgagc ccatcaccgc acctaacccg ccacgcgcag caatacgcga
     4981 agggatttta tctttgtcta gattctgaat ccctgctgac ttcatacgca tctcgccacc
     5041 aggaccgatg agtgaaatgg caactttatc gccatatttt tcgatcagtt tcggagcagt
     5101 ttcatacaca cccaagccaa caagctcatc ggctcgctcc cattttgcgc catcgttcaa
     5161 agacaagtgc aaaacccagt agccatcttt ttccggcata tcttcaagaa tgagtgcatg
     5221 gatacccata aacgccatgt gatagcccgt gcgcccgccc gcattggctt ccttgattcc
     5281 gcccgtcagc ggagacttac cgcctaccga aatacggtct gtggaggaaa ggatatgccc
     5341 tactaacaag ccgggcgtaa aaatcaactt attgccgggt ccaagtgggt cacacgttgc
     5401 atccacttca tccaccatga tgcgtgcaat gagtccgcgc ccgccgaggc gttgccaggt
     5461 ttcagggaca ggttcacgct tcaattcttg agtacgagta ttaatacgcc agatttgaga
     5521 tttatagtca gtcattatca ctctccgaac aaactcttca cagcttctct aaaaactttt
     5581 gtatgatgat ccacatcatc ttgagtggtt tcaggtgaga tgagcgccat gttatgaaag
     5641 ggcgtcatca atataccgcg atttagcgca tacaagtgca tgtaacgatc caattctgga
     5701 tctacggcag catgagcttc gccgccgttc tttggaggtg tgggacggaa ccagtattca
     5761 gagcgatttc ctaaacgctt cacaatccac ggaagcttga attctgcaat cacgccttcg
     5821 actcccgctg taaactgttc ctgtaacgtc gtcgctttgg catagaactc atcagtgagc
     5881 acattctgaa gtgttgcctt catcgccgca atggacagag cattacctgc caatgtgcca
     5941 ccaataccac cgacatcggc atcgtccatg gcaagtttag cagaaaatgc tttcgagact
     6001 tcctctgtaa aaccatagat cgcggcggga acgcctccag cgagaggttt gccgagggtg
     6061 atgaaatcag gctcaagacc gtaagaggcg gtgtatccgc cgggaccagc acaaatggtg
     6121 tgcgtctcgt caatgatgag ataggtcccg tatttgcgcg tcaatgcacg caaagcttca
     6181 tgatagccgg gatcagggtg gatgatgcca atattcgtca tggcaggttc ggcaagtaca
     6241 actgccacat cgcgagcgga aagcgccgtt tcgagggcgg caatatcatt gaattcaatg
     6301 accttgctag tttcgcgcgg gtcgatctgt ggacccatat tgtttgggcg agagatgggc
     6361 gttccttctt catcaagcgt gatgaaggtt tcatcgacag aaccgtgata gcaatagttg
     6421 aaaactaaaa ccttggggcg ccctgtgata aggcgtgcca tgcgaagcgc aaagcgattc
     6481 gcatctgtag cagtaagagc aaattgccaa tacggcaact tgaagcggcg ttgaagttcc
     6541 tcacctaccc aaatgacatc ttcatagggc aacattaggg taatgccctt ttgaatttgc
     6601 tcagtaatgg ctttaaccgt cgcttcggga gcgtgacctg tcatggcgcc cgtatcgccg
     6661 agacaaaagt cgatatagtt gttgccatcc acgtctgtaa aatgtgcccc tttggcttct
     6721 ttgacaaaca ccggaaaaga ccccgcccag cgaatcatcc acaacatggg cacaccacca
     6781 tgcagagatt tgcgcgcccg ttgatacagc tcatacgatt tggggtgggt cttatgaaag
     6841 agttcttctt cttgtttgag aagtttttcg agtttggaac gatcaagggt agtgttcatg
     6901 gttctctcta ttttttcagg attgtcaaaa tctcaaatcg actgtatatt accgaagcga
     6961 gggggcggac tatcaacccg tgcgccgcct ctatttggag aaaatactat atatgactga
     7021 aacgccttct gccaacctgc tcgactcatt agacttgaac aaacgtttca agacttctgc
     7081 ctatttcacc atctgtttta actcattgcc ttcgagcttg caccttgtgt tatccagcga
     7141 caagaaacaa gatgccgtgg atgccatcct tgccgaaatg gagttggaaa aattcaactt
     7201 caccaccgtg ttcaagaatt ggtctactga agattttcag aattcacaac ccagcgacga
     7261 ctatgtgttc gagtatgttt tcaaaagtga agctgggcgc atgctcattc actttacggc
     7321 tcgcgacctg tcgtacatca cagtagaatt tctttacgac catgatcaaa aaggcagtga
     7381 agaatgggta ctggcgatga accacaaatt acgctcaaag tttggagcac ccaaaaagcc
     7441 acaattcaaa gtccttatgc atcaggacgg aatgttcggt atggaagaag tgagcacaaa
     7501 tgatttcaag tccatcgata ttgccgaact ctacaatgat gattttgtcg aagtggataa
     7561 agccatttcc acattcatgg gcaagcaaga gtctggcatc atccttttgc atggtgagcc
     7621 cggaaccggc aagaccactt acatcaaaca cctgatctgc aagtttatcg accgcgaatt
     7681 catctttatt caaaatgatt tcgtcagcga cttgttgaaa ccaaatttca tagcttttct
     7741 actgcaaaac aagaatgcag ttctcatcat tgaagatgca gagaaagttg tcgtgccgcg
     7801 agaacactcc tctgaagcat ccgtcgtctc aaccattttg caattgaccg acggtctctt
     7861 cagcgacttt ctcaacatca agatcatctg caccttcaac accgatatcg agcgcattga
     7921 caaagctctc ttacgcaaag ggcgtatgat tgccaagtac aacttctctg cgctctcccc
     7981 tgcaaagacg gctgccctcg cccaaaaact aggtcacgaa catgtggaag gcagcctcac
     8041 ccttgcagac attttcggct atgacaaact cgatttcaaa gaagccacca aacgcaagat
     8101 cggtttcgtc taaacagagc cttttacagc ctcgccaaaa atcttcaaac cagcgttgat
     8161 ctgttcagaa gagacattca atggcggaat ccaacgcaaa atattatcgt aggtaccaca
     8221 ggagagaagg agcaaaccct tctcttctgc tttatggatg atctctttga ccaacggctt
     8281 ggctttcgcc tgactgccat ccacgataaa ttcggtgcca accatcaagc ctttaccgcg
     8341 cacatcacca atttgcggat attcttcctg caatttatga agcccggtca tcaattggat
     8401 tccgcgttcc gtggcgttct ccaacatctt ctcttcacgc atcgcgcgga tggtagcaac
     8461 accagccgcg caggcaactg cgttcccacc ataggtccca ccaatggagc cagtatccac
     8521 tttcttcatg atctcggtgc gactaaagac gcccgaaagc ggcattccag aggcaatccc
     8581 ttttgcagca gtgataatat ctggaacaac accaaagtgt tccaacgcaa accacttgcc
     8641 cgtgcgacca aaaccagatt gcacttcatc gaaaatcagc ataatgccgt gcttgtcgca
     8701 gatctcacgc aatcccttca tgaaggaggc aggcggcacc acatacccgc cctcacccaa
     8761 tacagactcg atcaagatag cggcggtctc tttaggagcc gtttgagaag ctagcaaata
     8821 ttccaattgc tctaatgcat attgactggc ttgttcttcg gtcatcttca ggttgaaagc
     8881 atatgggaac ggcgctacgt aaatgccagc aggcaaaggc gcaaagccgg tgcgatagat
     8941 tgtcttagag gtggtcaatg ccatagtggc atgcgtgcgc ccatgaaagg agccgttgaa
     9001 gacaataatg ttttgcctac ccgtgacagc cttggcgatc ttcacggcat tttccaacgc
     9061 ctctgcgcca gagttggcaa agtagaaact atcaatcgaa ggcggaacta ctttacgcag
     9121 ttcttcaatc agttgcaaca tcggtttgtg gatgacgatg ttcgcctgag catgcaagaa
     9181 caagccagcc tgctcacgaa tcgcctccac cactttgggg tgacaatgcc ccgtgtttgt
     9241 gacgccaatt ccactggtga aatccagcag tttcttgccg tcatcggtat aaatatatga
     9301 accctcagca cggtccgcta cgaaattgaa aatgcggctc caggcagggg ttacatgaga
     9361 aaaattatct tgatagagtt gattggtcat gatttcctta ttgaccagaa agagcacaga
     9421 gttttccctg aatttcttct tggactctgt gctctcagtg atcagattgt atttacatta
     9481 tgccggaagg tattccgctg cccactctac atcatgcttc ttcaaagttt cgcgggtagg
     9541 aatgccgtta ttcgtccaac ctgccatctt gtaataggta tcgagagcag attcaacttc
     9601 ttcatgagtc agagcgaagc ccgctgtggg acctgtcccc ttcaattcct tgaagaattt
     9661 cttaggcaat ttatccgcat tgcgagccaa accttcacga gcattgaaga cacggaagag
     9721 atccaaacgt cggcgcccga cttccatcaa ttcgtttagg gtaatatccc aacccgttac
     9781 cgagctgatc atctgtgcag tttgttcgcc atcatagacc gtccatgtcg gaccgtacac
     9841 aaactggcag agttctgctg agtcgagcat cgagtaaaag acttcagtct cgtatgcgaa
     9901 gcggactttt tcctctgtaa gtgagtatgc aggttggggc gaacccaaac caatctgttt
     9961 gaggcggtcg aggttgaaat cacctacacc ttcttcatag tacgggtcgt gttcagaaga
    10021 ttggtgatct gcgccgaagg gattgaccgc ataaatgagc gccaacgagc gtttggcttg
    10081 cggcatgtgt gcaggagctt cagcgccttt gaccgtgatg aggcattcat ctgcaccatt
    10141 tccccacttg gcggcagcac gttctgagcc caaaccaagg gttttgccga attcaccctc
    10201 acccttcacc agcatattca aagcagcgat cattgcttcg gcattaccaa atttcaagtc
    10261 aagaccagcg gtctgttctt tggtgataat gcctttttca tagcactcca tcgcaaaagc
    10321 gatggttgca ccagccgcaa ttgtatctac ggcatattca ttacaaatct ggttggcaag
    10381 agataccgcc gcaagatcat ccacaccgca gtatgaacca aacgtgccaa gagtttcgta
    10441 ctcaggacca ccatagcgcg gatcaacctt ataaggaccg tctttgatct ctaccacgcg
    10501 tttgcatttg accacgcatg catagcaagt atcacgttcc ttcaagaccg tctcagccat
    10561 tttttcgccg ctgatcggtt cacaaccttc gaactgacct tcgttatagt tacgggttgg
    10621 caatgtacca atggtgttat tgaacaatac caccactgcc gtaccgaatt tcgcaagccc
    10681 gtccatatca ccattttcag gaatggcttt cggaccgata cgatttaact cagtaagagc
    10741 cttttgattg gcaagtttaa tcttgccggt acctcgcacc accacagcct tgagattctt
    10801 agatgccatc accaaaccca tgcccgtacg cccattatga cgattagaca tagaaacgag
    10861 ggatgaatac agtaccccat tttcagcgcc aatcccatgc tgaaggatct cagccttcgg
    10921 gtccacttct ttgtggatga tatcgtccac ttcgcccgaa agcttgccca gcaaatgcga
    10981 ggcgtcgcgt aattcaaatt ttccatcaat aatgcagaga tatacaggct tgggtgactt
    11041 ccccttcaca acaatgccat caaaaccggc aaacttcaat tcagccggga agaacccacc
    11101 gctctgcgaa tcaccaatac cgccactgat cggcgacttg gcatttgcat tcaaacggct
    11161 ctgacctgag attgccgccc cagttgtaac accggtaaac aaggtcaaaa cattttcagg
    11221 tccaagcggg tcagcgccct tcggcatatc gcgcaagatg tagtgcattc ccattgcgct
    11281 cccgcctaaa tacttgcggt aaaaagcctc attcggctcc tccaccgtca gagagccctt
    11341 cgtcagatca acgtgcaaaa tctttccgtt ataaccgttt ggcatggtta ctcctccgta
    11401 agaattgatg atggtaatta taacaagcgg gtcttacttc tcacaacagg cagtttccga
    11461 aagcgacagc ttaaccgctt cgattaagtc ttttcctaca ctccccgccg cgcttccttc
    11521 ctaactgaca gcggataccg ctttctcgaa cctttcacac ccgaagcaaa agggcgtccc
    11581 gtaaagagtc gcccttttat aaactacata ttcttaatgc cgtatgggaa gccgtaatct
    11641 gccatttttt ccatgaagac agtcggctca aacgcctcag gtccgagcac acctgtgccc
    11701 ttccaagcgc catgttccag caaatccata gcaatcacag tggtaaatgc cgtttgagcc
    11761 accactgcct ggcaccccca agtcttcatg cattcttcgt tatccgcaac ctgatacaga
    11821 tatacttctt tcttcttccc atcttttgtg ccttttaccc aggttccggc acatgtcttg
    11881 cctttcatct tgtcacctaa gtgagctggg tctggtaaac aagccgccac cacatctcgc
    11941 ggcgcaactt gcacgccttt cacattgatc ttgttcttat tatccaagcc aagtttatga
    12001 agggttttca acacttcaat aaattcattc cccaacccat acttgaaggt cacacgcttt
    12061 gccttcaccc agcgcggcac gagcaatact tcctcatgct ccacgttgac gacttcaacg
    12121 ggtccaattt cagggaagtc gaacacttca ggctcagaga acggagccgt ggtatgccaa
    12181 tctccgtttt gccagatgac gggcggattc aaacattctt caatagttgt ccaaattgaa
    12241 aaattcggcg caaaatcata gccttcaatg gtgatattag cgccatcgcg aatgcccact
    12301 tcttcgatct cgtcaaagag atggtcagct gcatagcgag caaagacatc tgccatgcca
    12361 ggttccacgc caaacccaac aagcgccaac aaacctttct tctcccactc tttggctttc
    12421 tcaaattgat aatcgcccaa cttaaccccc ggtttattga acgggtcggt tgggtgcggc
    12481 tcggaaaggg tcatcgccat gtccatatat ttcacgccag cattgtaggc ggcatcgaag
    12541 atctgttggt tgaagatcgg gtctactgaa ttcatgatga gatccacttg gtgtttcttt
    12601 gccaagtctt cgatattttt ctggttgcta gcatcaatga attcgaccgg gaagcgttta
    12661 gggtctccca gcttcttttg cacttcttct gctcgtttga cgttgtaatc tgccagcacc
    12721 atcaactcca tccactcgcg cggcttggca atggcggcta tggcttcccc aactccaccc
    12781 actccaacta gcaatacttt catgagatct gattctccgt ttggttttat aacccgcttt
    12841 tgcgagttag attgactgaa aaaagaatcc cgccgcataa aggcggcggg acagactgaa
    12901 gctaccaatt gcgattcata tcgctggctt gatttcctgg aaaaatggaa atcatgagtg
    12961 aagtcatata aaccttcaag aaggtatccc ccacagtctt tagtacacga gctagggctt
    13021 tcattagaac ctttcagttg gctctgcgtt ctggattcgt ttccagtgca gacaaagttt
    13081 tggaacgctg cagcaggcat aaggtccata ctgaagcgga attcccatta gaaataaatc
    13141 tctttggcga tcttcaagta aatgaaggat tcggtttcgc gaacgccatc cacttttgcc
    13201 agtttttcag ccatgaaatt gaacaaatcc tcatggtcgg cacaaaagac ttcgatcatc
    13261 aggtcatagc gaccgctggt ggcaacgatg tatacgactt cgtcaaattt cgcgatcttg
    13321 ttcgccactt ccatcatttt gcgtccatcg gtacgaatac cgatcatgga catagtgcgt
    13381 ttacccatca tgagcggatt ggtaaccgcc accaccttga ggtagccaag gtcaaccaga
    13441 cggttatagc gttgccgtat catgccgggc gacacattga gctgttcggc a
//
LOCUS       DAOQNJ010000009        13014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_2037_length_13014_cov_8.088510, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000009 DAOQNJ010000000
VERSION     DAOQNJ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13014
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2037_length_13014_cov_8.088510"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLG52_00595"
     CDS             complement(<1..187)
                     /locus_tag="PLG52_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HNB39964.1"
                     /translation="MPNLTERQKTLLMLIIRDYIDTAQPIGSKRLVEHYHISLSSATI
                     RNEMAALTEMGYLRQPHT"
     gene            complement(322..2559)
                     /locus_tag="PLG52_00600"
     CDS             complement(322..2559)
                     /locus_tag="PLG52_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB39965.1"
                     /translation="MTLDKGTLIHNRYRIVDILGQGGMGSVYRAIDENLGVAVALKEN
                     LFTTDEYARQFRLEAVILANLRHPNLPRVSDHFVLGDLGQYLVMDFIEGEDLRYRMER
                     LGMLEEEDAVHIGAAMCDALAYLHTRKPPILHRDIKPGNVKISPDGHIYLVDFGLAKV
                     YQSASQATTTGARAMTPGYSPPEQYGTARTDPRTDIYSLGATLYAALTGVIPEDGLAR
                     AMDNAQLTPLRKRNSKVSRKLAASIEKAMAVDPSDRFQTAEEFKAGLLSSKSMTQQAP
                     GSYKVIPPPPDAVAASSHTHEVTVVDEALPVDGKKDRKALVGPVPAAGSNAHEDQPFV
                     SPLKRQKERERKRRMALWRFALFAGILLLVLGSVVFFVPGVLPADVRAMVPFIGATAT
                     PTFTLTPVPTQTLPATFTATLEPTSTPTLRPTNTPTATFTPTPLPGETITITPVASSG
                     GTEVFMGGGAGQIAFASDRTGAPQIFLVDLRGESAFQVTNMQGGACQPAWSPDGLRIA
                     FISPCKGNEDIYTNTSLYIINADGTSLATLDASPGGNFDPAWSPDGKTIAFTSARTGQ
                     MEIFMLPVDDPESITQITSGAAKVESRMPTWSPDGSKLVYVVKRVGVYQIWMMNADGT
                     DAAQIIRSGQAYNDYSPVWSPRNDLIIFNQRCAKTYCNPYLLSTSPTDRSKEQGLKLP
                     FNVIFIEDIDFSPDGFYLAYEGVGDSNNFDIFFMTLSGGDKVRLTTDPANDFQPTWRP
                     VIQNP"
     gene            complement(2651..3646)
                     /locus_tag="PLG52_00605"
     CDS             complement(2651..3646)
                     /locus_tag="PLG52_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNB39966.1"
                     /translation="MQTLIDFGVSFIIALQRLGEWQIPIMAFFSRLGTEEFFFIVLPL
                     LFWNIDSALGIRVAFILAVSNFINLIGKVGFAGPRPYWVSSHIQPWWPETSFGIPSGH
                     AQHAMSVWGIIALYKKEKWITIVCVILIFMIGFSRLVLGAHFPHDVILGWLLGAFLLW
                     LYVRYWDATVIWLSNMNFSKQVGVAFLVSMIFVVIGMIVTNTRSDFQIPESWMTNALR
                     SGIAPDPIDPNGVFTAVGTFFGLAVGAAWTHLRGGYQVDGPVWKRVLRYVIGLAGVLI
                     LWQGLGVIFPRGDGLVVYSLRFVRYSLVGWWISGGALWVFKHFNLTTPSSRPTSI"
     gene            3718..6081
                     /locus_tag="PLG52_00610"
     CDS             3718..6081
                     /locus_tag="PLG52_00610"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HNB39967.1"
                     /translation="MEKTSVRNWDWISNLIMLFAVLFSAWRLQTADWTEGLGQVRNVA
                     VLGFALGLGVGYSQFKRKPIFFLVAGYTVAILIWQLLGTIPFDKDQTYLLDKFSILFG
                     RIFTSLGELFAGRAVEDQFFVVALLCIPYWFASLYSGFQLTRRSNFLGTILPNGVLML
                     IIHLYHYTNQDYTWMLGVYLFLALLLLSRLKYLADRRKWSKERIQVQSDSGLDITNTT
                     VAVATVLILLAWGSPYILPATTEGKQIWREVYGQIFPKDRFKNVFASVEKENQPKARN
                     FRPELALGTQTPQGNSIVFQVYVPLEAKKFPRLYWRGQVYDRYENGRWLTTSDAEVRQ
                     NPATGDITIPDSADRQRLSFTFDVMTSGQTTVYLPPQPIWINHNTILLSSDISNKTGE
                     DAILDIMAVRASPPFQDGDLYRVSSLLADPTAEELRLSGDAYPDWVKEKYLQLPENFS
                     PRIQALAQQITAPYNTPYDKTVAVTNYLRREIEYTGVIYLPNEDVDPLEYVLFDAKRA
                     FCNYYATAQVLMLRSVGIPARLAVGYAQGQPNIQKSLYAVREKDLHAWPEVYFPEYGW
                     IEFEPTTNQQPIERPEAREDLAQIAPFANNSNQPLFKPDVEFTPDQEPNVPDGGSASA
                     RSWLDWLASLLPWLGGVFFILLGIVLKRRFAPQVTVASVMKRAIDRSGWNAPRWLRRW
                     LAFATQPAIQRHFHNINISLRLLKRPQPVHVTAAERAQTLKGLLPSASASIDSLLNEL
                     QAELFTPNGGNEAIARRAAWDILSKTVQSKLKIIILGYNYEGIKETS"
     gene            6088..7800
                     /locus_tag="PLG52_00615"
     CDS             6088..7800
                     /locus_tag="PLG52_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB39968.1"
                     /translation="MIENSTRPRTVHDQLSALAVLAGKIRAELGNAKLKLPKESLEQL
                     HYLQSTLETVGNKMEVFQREHGNMLALVETGQVINSSLELDEVLRIVMDNIVRLTKAE
                     RGFLMLRNEGGEMINRVARNWEMESINPSEKNVSRSVVQRVIDTGESVVTTNAQEDQR
                     FVGQESIVAFNLRSILCVPLKVKNDLIGVIYADNRIRAGIFAEAEKDLLEAFANQAAI
                     AIDNARLFSSLKGTLEEVTALKNLMDNVFASIASGVITADVQDTITLANRAAETILGS
                     PTQDIIGHHLKDALASVSGELAPHLMEVRKTDKPIVDLEISHNLPKRGNVDWRLNLSP
                     LKDAGQKTQGVAIVLDDLTERKKLEAQRRLFERMVSPAVIEQLDPNGLQLGGKRTEIT
                     ALFADIRGFTTYSEHLAPERLVSILNLYLAAMADAVLAQEGTVDKFMGDAIMAWFNAP
                     IPQPDHTLRAVKTALAIRESIENLYKELSSDSHLAFGVGIHFGDAVLGLIGTEKRLEY
                     TAISDTVNTAKRLQENSARNQIIISQDAYERVKNDVNVKPISEITVKGRSKPIEVFEV
                     LGLK"
     gene            complement(7849..8937)
                     /locus_tag="PLG52_00620"
     CDS             complement(7849..8937)
                     /locus_tag="PLG52_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB39969.1"
                     /translation="MTLAKKVLDLAIQIQQIASPTFAEGPRGEFIRDLFIQEKLDDVS
                     MDSLGNVYARLPGKQKKARALIVSAHLDTVFPAGTKLQIKEEAGKVFGPGIGDNSIGV
                     AALFGILWGIRERKIELKHDVWFVANVGEEGLGDLRGMRGVVERFGNDELGYLVLEGL
                     ALGHVYHRAIGVRRYRITAKTRGGHSWSDYGQPSAVHELAAMVTQLTQVRLPREPRTT
                     MNVGTIHGGTGVNVLAAEAKCELDLRSEDPVVLAKIVSQVEDIILHWNHEGVKIQAEI
                     IGERPAGQVASDHPLVKLAVESLNAQGMEAILTTGSTDANVPLSKGIPALVMGITTGG
                     GAHTMGEYIDTEPIERGMESLIRFVELS"
     gene            complement(8980..9990)
                     /locus_tag="PLG52_00625"
     CDS             complement(8980..9990)
                     /locus_tag="PLG52_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNB39970.1"
                     /translation="MTSNFALAGKLTREYLLPLNGSPRLDSPGGNLLYAMSGLAVWDT
                     SISLIARVNREYPQEWISDFQTRGLDVDGIYVDPESDRVDIRSFIAYTDTNERSITNA
                     VSHFARREMTFPKSLLGYHPQDPTIKDLRETDPLSPAARFVPKEYRNIGFVHLCPFDF
                     TSQSQLVNLFKGGSNQMVSLDPAPGYMKPKFWRELRVVLQGVTAFLPSEDELRSLFWG
                     ESHDLWEMAQRISEHGPQIIVIKRGPQGQLVYDAASKRRYEVPAYTARIMDPTGVGDA
                     FCGGFLAGYQRTNDPLQAALHGNVSASLKIEGVGVYATLDAMPGLADARLHALKEMAR
                     EI"
     gene            complement(9996..10559)
                     /locus_tag="PLG52_00630"
     CDS             complement(9996..10559)
                     /locus_tag="PLG52_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNB39971.1"
                     /translation="MTTYCDYCNSHPEDTFNKTYHDTQYGFPIDDDNELFERLVLEIN
                     QAGLSWILILKRAQNFRKAFHKFNIKKVAKYTEADRERLLNDEGIIRNKLKVNAAIVN
                     AQKILELQKEYGSFKGWLDAHHPLTKDEWTKLFKKTFVFTGGEIVNEFLMSTGYLPGA
                     HQKDCPTFKKVAAKKPAWMQPSKARKS"
     gene            10626..>13014
                     /locus_tag="PLG52_00635"
     CDS             10626..>13014
                     /locus_tag="PLG52_00635"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39972.1"
                     /translation="MPIPVMTTKLRVPPLRTKVVIRQRLLERLQEGLNHSLTLVSAPA
                     GFGKTTLLSEWLGEVNLKVAWLSLDDSDHDTIHFITYLVSALQVIDKSIGESVLGALK
                     SHQPPAVDTLLVALLNDIDSVSQDFILVLDDYHEVDSKPVDEALRFLVEYMPPQMHLI
                     IASREDPPLPLARLRAKGQLTELRAADLRFTMQEAADFLNQVMGLDLSGQDITALETR
                     TEGWVAGLQLAALSMQGHDDIRGFIQSFTGAHHFVLDYLLEEVLSKQPQEVQHFLLRT
                     SILERLNGPLCEAVTGMPSQKMLEELERSNLFIVPLDGERNWYRYHHLFGDLLRQRSE
                     QHIASNEVAELHLNASAWFEQNGDLADAFRHAMAAKDQNRAADIAERAWLGMDETFQS
                     GQWLGWVKQLSGSVRRLRPVLCVQIAWAYMDASKADESESFLQEAEQCLREAAHTMVV
                     VEEEQFKTLPARIAIARAYNSQTKGESLATVKLAQEAVRLSPETNQFMRAQATAILAG
                     AYWANGELTAAYDAMNHWAEQARLAGNTMFAIMTASGLADILQEQGRLRNAMQIYEDA
                     LKFAADVGAEVVPFTAHHHLGMALLLNEMGANDAAEKHLQLSLELGQAATIIDWQYRR
                     NFALARFKETAGDLNAALYFFDEAKRTYVKTPIPLTRPVDAFKVRVYLKQGLLAQTHE
                     WAQKFSMDDELSFLHEFEHITFARVLLAKKDSKNVSQFLARLLQAAEDGARIGSMLEI
                     LILQALALHAQGQLTQALLPLKRAVALAEAEGYVRIFEEGKPMQELLAKLSLTPEG"
BASE COUNT         3572 a         3066 c         3065 g         3311 t
ORIGIN      
        1 atgtatgcgg ctgacgcaga tagcccatct cggtcaatgc cgccatctca tttcgaatgg
       61 tcgccgacga caggctgata tggtaatgct ccaccaaccg cttcgagccg attggctgag
      121 cggtgtcgat gtaatcacga atgatcaaca tcaggagggt cttctgtcgt tcggtgaggt
      181 ttggcatggt caaaaattta gcactccaaa tttcagagtg ccagtttata acggaataat
      241 aatatcagcg gggtatggag gcgtcaataa gaggagtgta agaattaata agaaaactcc
      301 cgcttgcgcg ggagttttta tttatgggtt ctggataacc ggtcgccacg tcggctggaa
      361 gtcgttggca ggatctgtcg taaggcggac tttgtccccg ccagaaaggg tcataaagaa
      421 gatatcgaag ttattcgagt ctcctacccc ttcatacgca aggtagaaac catccggcga
      481 gaaatcgatg tcctcaataa agataacatt gaacgggagt ttcaacccct gttctttgct
      541 acggtctgta ggggatgtgc tcaataaata tggattacag tatgtcttgg cacagcgttg
      601 attaaagatg ataaggtcat ttcgcggcga ccagacggga gaatagtcgt tgtatgcctg
      661 tccactgcgg atgatctggg ctgcatcagt tccatcagca ttcatcatcc aaatttgata
      721 gacacccaca cgtttgacta catataccag ctttgatcca tctggtgacc aagtcggcat
      781 gcgagactca actttagcgg caccacttgt aatctgagta atagattctg gatcatcgac
      841 tggaagcatg aaaatttcca tttgtccagt acgggcagac gtgaatgcaa ttgtttttcc
      901 atcaggcgac caagcagggt cgaagttacc accaggggaa gcatctaagg ttgctaaact
      961 ggtcccatcc gcattgatta tatataagct tgtattggta tatatgtctt cattcccctt
     1021 acatggagaa ataaaggcaa ttcgcagacc atcaggtgac catgccggtt ggcaagcacc
     1081 accctgcatg ttagtgactt ggaaggcact ttcaccacgt aagtccacta aaaatatctg
     1141 aggagcaccc gttcggtcag aagcaaaggc aatttgccct gctcccccac ccataaatac
     1201 ttctgtcccc cctgaggaag caactggtgt aatggtaatt gtttcgcctg gcaatggtgt
     1261 tggggtaaaa gtagcagtag gggtgttagt cggtctcagt gtaggggtag aggtgggttc
     1321 caatgtagcc gtaaaagttg ctggcaatgt ctgtgttggg acaggtgtaa gtgtaaaagt
     1381 tggcgtcgct gttgccccaa taaaaggtac catagcacga acatctgccg gcaaaacacc
     1441 tggaacaaaa aagacaacac tacctagaac taacaataaa atacctgcaa aaagtgcaaa
     1501 acgccataaa gccatacgac gtttacgttc tcgttccttt tgtctcttta aaggagatac
     1561 aaaaggctgg tcttcatgtg catttgaccc agcagcaggg acgggtccta ctaaagcctt
     1621 tcggtctttt ttaccatcta caggcaaagc ttcatcaacc actgttactt catgggtgtg
     1681 agatgaagca gcaacggcat ctggcggagg cggaattacc ttgtaagagc cgggtgcctg
     1741 ttgcgtcatt gacttagatg aaagcaaccc agctttgaat tcttcagctg tttggaaacg
     1801 gtcgctaggg tcaactgcca tcgctttttc aatagaagca gctaattttc gtgaaacctt
     1861 tgaatttctc ttgcgcaatg gagttaattg tgcattatcc attgcacgcg ccaaaccatc
     1921 ttctggaatc acaccagtca atgcagcgta taaagttgcg cccaaagagt aaatatcggt
     1981 acgcgggtca gtacgggcag taccatactg ttctggaggg gaatagccag gggtcatggc
     2041 acgagcaccc gttgtggtag cttggctcgc gctttgatat acctttgcca acccaaaatc
     2101 cactagataa atatgaccat ctggcgaaat tttgacattg cccggtttga tatcacgatg
     2161 aagaatgggc ggcttacgag tgtgcagata cgccaaggcg tcacacatgg ctgcgccaat
     2221 atgaacagca tcttcttcct ctaacatacc tagccgttcc atacgatagc gaagatcttc
     2281 gccctcgatg aagtccatga ccaagtattg ccccaaatca cctaaaacaa aatggtcaga
     2341 aacgcgtggc aagttgggat gacgcaaatt tgccaagatt acggcttcaa gacggaactg
     2401 acgagcatac tcatctgtag taaacagatt ttctttgagt gctacagcca caccaagatt
     2461 ttcatctatg gcgcgatata cagagcccat cccgccttgc ccaaggatgt ctacgattcg
     2521 ataccgatta tgtatgagag tgcctttatc cagcgtcatt gaaatccatt cacctgcaat
     2581 atataaattt ccagcaaaga ctgtagattt aaaaaaatgt tactactttg atttggacac
     2641 gcgcattata tcagatggag gttggacgag aagatggtgt agttaaattg aaatgtttga
     2701 aaacccagag ggctccacca gaaatccacc accctaccaa ggagtaacgg acaaagcgaa
     2761 gagaatacac caccaaacca tcaccacgag gaaaaataac accaagccct tgccacaaaa
     2821 tgagaactcc agccaaaccg atgacatagc gaagtacacg cttccaaacc ggaccgtcta
     2881 cctgatagcc acctcgaagg tgagtccacg ctgcacctac tgccagcccg aagaaggtcc
     2941 caactgcagt gaagactccg ttcgggtcga taggatcagg cgcgataccg gagcgaagcg
     3001 cgttcgtcat ccatgattct gggatttgga aatcacttct cgtatttgta acaatcatcc
     3061 caataaccac aaaaatcata gaaactaaaa aagcaactcc cacttgcttt gaaaagttca
     3121 tattggaaag ccagataacg gttgcatccc aatagcggac atatagccac aacaaaaagg
     3181 cgcctaacaa ccaaccaagg atgacatcat gcggaaaatg tgcgccaaga actaaacgcg
     3241 aaaagccgat catgaaaata agaatgacac agacaatcgt gatccatttt tcttttttgt
     3301 acaaagcaat gatgccccaa acgctcatgg catgttgtgc gtgccctgaa gggatgccaa
     3361 acgaggtttc gggccaccat ggctggatgt gcgagctgac ccaatatggg cgcggaccag
     3421 caaaaccaac tttaccaata agattaataa aattgctcac agccaaaata aaagccactc
     3481 gaatgccaag tgctgagtca atgttccaaa aaaggagcgg caaaacaata aagaaaaatt
     3541 cttctgtgcc taatcggctg aagaaagcca tgatgggaat ttgccattcg cccaaccgtt
     3601 gaagtgcaat tatgaaagac acaccaaagt caataagggt ctgcatctag tcctccagta
     3661 gggtttttct taggaagtga attgacgtaa caatgcgacc cattgtacac tcaaagaatg
     3721 gaaaagacat cggtgcgtaa ttgggattgg atatccaatc tgattatgct ttttgcagtg
     3781 ttattttctg cctggcgctt acaaaccgca gactggactg aaggcttggg gcaagttcgc
     3841 aatgtggcgg tacttggttt tgcacttggg ctcggggtag gatacagcca atttaagcga
     3901 aagcctatat tttttctggt tgcagggtat acagttgcta ttttgatatg gcaattattg
     3961 gggacaattc catttgataa agaccaaact tatttattag ataaattctc gatcttgttt
     4021 ggaagaatat ttacatctct tggtgaactt tttgctggta gagctgtgga agatcagttc
     4081 tttgtcgtag ctttattatg catcccttat tggtttgcta gtctttatag tgggtttcag
     4141 ttaactaggc gttctaactt tttgggcacc atccttccaa atggtgtgtt gatgctgatc
     4201 atccatctct atcactatac caaccaagat tacacttgga tgcttggggt ttatcttttc
     4261 cttgcattat tactactcag ccggttgaaa taccttgcag accgtagaaa atggtcaaag
     4321 gaaagaattc aggttcaaag tgatagtggg cttgatatca caaatacaac cgtggctgtt
     4381 gccaccgtct tgattttatt ggcatggggg tcgccatata ttctgccagc gacaacagaa
     4441 ggaaagcaaa tttggcgcga agtgtacggg cagatctttc cgaaagatcg cttcaaaaat
     4501 gtatttgctt ctgtggaaaa agaaaaccag cctaaagcac ggaacttccg cccagagtta
     4561 gcccttggca cacaaacccc acaaggcaat tcgattgtct ttcaggtgta tgttcccctc
     4621 gaagcaaaga aatttccgcg cctctactgg cgcggacaag tgtatgaccg ttatgaaaac
     4681 ggacgttggc taacaaccag tgatgcagaa gttcgtcaga accctgccac tggtgatatt
     4741 actatccctg atagcgcaga ccgtcagcgg ctttctttca cctttgacgt gatgacctct
     4801 gggcagacga ctgtttatct tccaccgcag cctatatgga ttaatcacaa cacgattctg
     4861 ctttcttcgg atatttcgaa taaaactgga gaagatgcca ttcttgacat tatggcagtt
     4921 cgcgcctctc ccccatttca agatggggac ttgtaccgcg tcagttcctt gcttgcagac
     4981 ccaacagcag aagaactgcg tttaagtgga gatgcatatc cggattgggt caaagagaaa
     5041 tatcttcaac tgcctgaaaa cttctcgcca cgcattcagg cactcgctca gcaaatcaca
     5101 gctccctata acacccccta cgacaaaaca gtcgctgtta caaactatct gcgcagggaa
     5161 attgaataca ctggtgtgat ttaccttccc aatgaagatg ttgacccgct tgagtatgta
     5221 ttgtttgatg ctaagagagc tttctgcaat tactatgcca cggctcaggt tctcatgcta
     5281 cgcagtgttg ggatccctgc ccgtttggcg gtgggatatg cccaagggca gccaaatatt
     5341 caaaaaagtt tgtatgcggt tcgtgagaaa gacctgcatg cctggcccga agtttatttt
     5401 cctgagtatg gttggattga atttgaaccc accacaaatc aacaaccgat cgaaagacca
     5461 gaagctcgcg aagatttagc tcaaattgct ccattcgcaa acaactcaaa ccagccacta
     5521 tttaaaccag atgtggaatt cacgccagac caagaaccaa atgtgccaga cggagggtca
     5581 gcttcagcga ggtcttggct agattggtta gcatcgctcc tgccgtggtt gggaggcgtg
     5641 ttctttatcc tccttggaat tgttctcaaa cgacgctttg ctccacaggt gaccgttgct
     5701 tctgtgatga aacgagcaat agaccgtagc ggctggaacg cgccacgttg gctgcgtcgt
     5761 tggctagcgt tcgccaccca gcctgcgatc caaaggcatt ttcataatat caatatcagc
     5821 cttcgattat tgaaacgccc acagcccgtg catgtgactg cggcagaacg ggcacagacc
     5881 ctaaaggggt tgctgcccag tgcgtctgca tcaatcgaca gccttttgaa cgagcttcaa
     5941 gctgagttat tcacccctaa cggtgggaat gaggcaattg cccgccgtgc agcatgggat
     6001 atcctttcta aaaccgtaca aagtaaacta aaaattatca tcttgggata caattacgag
     6061 ggtatcaagg aaacctcgta acaccccatg attgagaact ccactcgtcc ccgcaccgtt
     6121 cacgatcaac tatctgccct tgccgtgctt gccggtaaga ttcgcgcgga acttggcaac
     6181 gcgaaattga agttgccgaa ggaatcgtta gaacagctac attacttgca gtccactttg
     6241 gaaacggttg gcaacaaaat ggaagttttc cagcgtgagc acggcaatat gttggcgctt
     6301 gtggaaaccg gtcaggtcat taactcctct ctagagttgg atgaagtgct gcgcatcgta
     6361 atggataata ttgtgcgcct caccaaagcg gagcgcggtt ttctcatgtt gcgaaacgaa
     6421 ggcggggaaa tgatcaaccg cgtggctcgc aactgggaaa tggaatccat taacccttct
     6481 gaaaaaaatg tcagccgttc ggttgttcag cgtgtgattg atacgggtga gtcggttgtc
     6541 actaccaatg cacaagaaga tcaacgcttt gttgggcagg aaagtatcgt tgccttcaat
     6601 ctacgttcga ttttgtgcgt accccttaaa gtaaaaaatg acctcatcgg cgttatttat
     6661 gccgataaca gaatccgtgc agggattttc gcagaagctg aaaaagattt gttggaagcg
     6721 tttgccaacc aagccgccat tgccattgac aatgcacgct tgttctcgtc tctcaaaggt
     6781 acacttgaag aagtcacggc tttgaaaaac ctaatggaca acgtctttgc ttccattgcc
     6841 agtggcgtca tcacagcaga tgtgcaagat acgatcactc ttgccaaccg agcagcagaa
     6901 actatcctcg gctcccccac tcaagacatt attggtcatc atcttaaaga tgcgcttgct
     6961 tcggtttcag gtgaattggc tccgcatttg atggaagttc gcaagaccga caaacctatc
     7021 gttgaccttg aaatcagtca caacctgccc aaacgcggga atgtagactg gcgcttgaat
     7081 ctctctccgc taaaagatgc gggacagaaa actcaaggcg tagcaatcgt tttagacgac
     7141 ctcacagagc gaaagaagtt agaggcgcaa cgtcgcctgt ttgaacggat ggtctctcca
     7201 gccgtcatcg aacaacttga cccgaacggt ttacaactcg gcggtaaacg cactgaaatc
     7261 accgctttgt ttgcagatat tcgtgggttc acgacctata gtgaacacct cgcaccagaa
     7321 cgtttggtga gcattctcaa tttatatctc gctgccatgg cagatgccgt tctcgcacaa
     7381 gaaggcaccg tagacaagtt catgggtgat gctattatgg cttggttcaa tgctcccatc
     7441 ccacagccag accatacatt gcgtgcggta aaaactgcgc ttgccattcg cgaatcgatt
     7501 gagaatttgt ataaagaact ctccagtgac tcacatcttg ccttcggcgt aggcattcac
     7561 tttggagatg cagttctggg tttgattggt acagaaaagc gccttgagta cacagctatc
     7621 agcgacacag tcaacactgc caaacgctta caagaaaatt ctgcccgcaa ccaaattatc
     7681 attagtcaag atgcgtacga gcgtgtaaaa aatgatgtaa acgtaaaacc gatctcagag
     7741 atcacggtta agggacgttc aaaaccgata gaagtttttg aagtgcttgg gttaaaataa
     7801 aacaaaaggc ttccaaatgg aagccttttt gattcgaagt aatggcactt aagacaattc
     7861 tacaaaccga atcaaacttt ccattcctcg ctcaatcggt tccgtatcaa tatactcgcc
     7921 catcgtgtgt gccccaccgc cagttgtaat tcccatcaca agggcgggaa ttcctttgct
     7981 caatgggaca ttggcatctg tagagcctgt agtcagaata gcttccattc cttgtgcatt
     8041 taaactttct accgctaact tgaccaaagg atgatctgag gcaacttgtc ctgcaggtcg
     8101 ttctccaata atttctgctt gaatcttcac gccttcatga ttccaatgca aaataatatc
     8161 ttccacttgc gagacgatct tcgccaatac aaccgggtct tcggagcgca aatctaattc
     8221 gcacttggct tctgcagcca acacattcac acctgtcccg ccgtgaatgg tgccaacgtt
     8281 cattgtggtg cgtggttctc gcggtaaacg aacctgcgtt aactgagtga ccatcgccgc
     8341 cagttcatga acagccgagg gttgaccata gtctgaccag gaatgtccac cgcgggtctt
     8401 tgctgtgata cgatagcgcc tcactccgat cgcgcgatga tatacatgcc caagcgccaa
     8461 gccttccaaa acaagataac caagttcatc gttgccaaag cgctccacca cgccacgcat
     8521 accgcgcagg tcgccgagcc cctcctcccc cacattcgcc acaaaccaca catcatgctt
     8581 cagctcaatc ttgcgttcgc ggattcccca aagaatgccg aacaacgccg ccaccccgat
     8641 ggaattatcc ccaatgccgg gaccaaaaac cttgcccgct tcttccttaa tctgcaactt
     8701 ggttcccgcc gggaacaccg tgtctaagtg ggcagatacg atcaacgccc gagccttttt
     8761 ctgtttcccc ggcaaccgtg catagacgtt ccccaacgag tccatggaca catcgtcaag
     8821 tttctcttgt atgaacaagt cacgtataaa ctcgccacgc ggaccttccg caaaggtagg
     8881 agaggcaatt tgttgaattt gaatagcgag gtcgaggact tttttggcta gtgtcatgat
     8941 ggctgcaatc gtaaatcgtc agtcacaaat cgtcaattgt tagatttctc ttgccatttc
     9001 tttcaaggca tgcaaacggg catctgccaa accgggcatg gcatctagcg tagcgtaaac
     9061 gccaactcct tctattttta gtgaagcaga gacatttcca tgcaaagccg cttgcaaggg
     9121 gtcgttcgtg cgctggtatc cagcgaggaa tccgccacag aaagcgtcac caactccggt
     9181 cgggtccatg atgcgggcag tataggctgg cacttcataa cgtcgtttcg aggcggcatc
     9241 atatacaagc tgaccttgag gtccacgctt gataacaatg atctgaggtc catgctcact
     9301 gatacgttgt gccatttccc aaaggtcatg gctttctccc caaaacagcg agcgcaattc
     9361 atcttcagat ggaaggaagg cagtgacgcc ttgcaacaca acgcgtagtt ctcgccaaaa
     9421 ttttggtttc atataaccgg gggcagggtc taaactaacc atctgatttg agccgccctt
     9481 gaaaagatta accaattggc tctggctggt aaagtcgaaa ggacaaagat gtacaaagcc
     9541 gatgttgcga tattccttcg gaacaaatcg tgctgcagga gaaagtggat cggtctcgcg
     9601 taagtctttt atagttgggt cttgagggtg atacccaagc agggatttgg gaaaggtcat
     9661 ttcgcgacga gcaaaatgtg aaacggcatt ggtaatgctg cgttcattgg tatcggtata
     9721 tgcaataaaa ctacggatat caacacggtc gctttcaggg tctacataaa taccatcaac
     9781 atcaagacct cgagtttgaa aatcacttat ccattcttgc ggatattctc gatttaccct
     9841 tgcaattaat gaaatagatg tatcccatac agccaagccg ctcatggcat ataaaagatt
     9901 cccgccgggt gaatccaaac gtgggcttcc attcagggga agaaggtatt cgcgagtaag
     9961 tttgcctgct aatgcgaagt tggatgtcat tcaggttatg attttctggc tttggagggt
    10021 tgcatccaag ctggcttttt ggctgctact tttttgaagg ttggacagtc tttttgatgc
    10081 gcgccgggca gatagcccgt ggacataaga aactcgttga cgatctcacc acctgtgaaa
    10141 acaaaggtct tcttaaatag cttggtccat tcgtctttgg tgagtggatg gtgagcatct
    10201 aaccagcctt taaaggaacc atattctttt tgcaattcaa gaatcttctg cgcattgacg
    10261 attgcagcat tgaccttcaa cttattacga ataatacctt catcgttcaa aagcctttcg
    10321 cggtctgcct cagtgtattt ggcgactttc ttgatattga atttatgaaa ggctttgcga
    10381 aagttctgtg ctcgtttcag gatcaaaatc catgaaagcc cagcctggtt gatctcaagc
    10441 acgagccgtt cgaagagttc attatcatca tcaataggaa agccatattg agtatcgtgg
    10501 taggttttat tgaacgtgtc ttcagggtga gaattgcaat agtcgcagta agtggtcatg
    10561 agtatatttt actatctcgc taaacccttg cggagattcc agctaggcac tttataatta
    10621 cccacatgcc gatccctgtt atgacgacaa agttacgtgt ccccccgctg cggacaaagg
    10681 ttgtgatccg ccagcgctta ctggaacggt tgcaggaagg gttgaaccac tcgctgacat
    10741 tggtttctgc accagcaggt tttggaaaaa caactttgtt gagtgaatgg ttgggagaag
    10801 taaacctaaa agttgcatgg ctgtctttgg atgatagcga ccatgacaca atacacttca
    10861 ttacatattt ggtctcagcg ttacaggtca tagacaagag tataggtgaa agtgtacttg
    10921 gcgcacttaa atctcatcag ccacctgcag tagacacctt attggtagcc ttactcaatg
    10981 acatagactc agtctcacaa gactttatcc tagtattaga tgattatcac gaagtagact
    11041 ccaagcctgt ggatgaagct ttgcgcttct tggtagagta catgccgcca caaatgcact
    11101 tgataatcgc ttcacgtgaa gacccgcccc tgccgttggc acgtctgcgg gcgaaggggc
    11161 aattgaccga actgcgggca gcagatttac gcttcaccat gcaagaagct gcggattttc
    11221 tcaatcaggt aatggggttg gatctttctg ggcaagatat tacagcatta gaaactcgta
    11281 ctgaaggttg ggtggcaggt ctgcaattgg cagcactctc catgcagggt cacgatgaca
    11341 tcaggggttt tatccaatcg tttaccggag cgcatcattt tgtgttggat tacttactag
    11401 aggaagtatt aagtaaacag cctcaagaag ttcagcattt tttgttgcgc acgtcaattc
    11461 tggaacgatt aaatggtccg ctatgtgagg ctgtgacagg tatgcccagc cagaaaatgt
    11521 tggaagaatt ggaacgctca aatttattca tcgttccatt agatggcgaa cggaattggt
    11581 atcgctatca ccatttgttc ggtgatttgt tgcggcagcg gtcagagcaa catattgcct
    11641 cgaacgaagt ggcagagttg cacttaaacg ccagcgcatg gtttgagcaa aatggtgatt
    11701 tggcagacgc attccgtcat gccatggctg ccaaagacca aaaccgagca gcagatatag
    11761 ctgagcgagc gtggctcggc atggacgaaa cctttcagtc tgggcagtgg cttgggtggg
    11821 tgaaacagct atcagggagt gtgaggcgtt tgcgtcctgt gttgtgtgtg cagatcgctt
    11881 gggcatacat ggatgccagc aaagcagatg aaagcgaatc ctttttacaa gaggcagaac
    11941 agtgtttgcg cgaagcagca catacgatgg ttgtcgttga agaagaacag ttcaaaaccc
    12001 tgccagctcg aattgccatt gcccgcgcct acaattcaca gacaaaaggg gaaagtctcg
    12061 caacggtgaa gttggcacaa gaagcagtaa ggcttagccc tgaaacaaat caatttatgc
    12121 gtgcgcaagc gacagcaatt ttagcaggtg catattgggc aaatggcgaa ttgactgccg
    12181 cgtatgacgc gatgaaccat tgggcagagc aagcgcggtt ggcaggcaat accatgtttg
    12241 cgatcatgac tgcatctggc ttggcagata tcttacaaga gcaagggcgc ttacgcaatg
    12301 ccatgcaaat atatgaagat gctttgaaat ttgcagcaga cgtaggcgcg gaagttgtcc
    12361 cttttacagc gcatcatcat ttagggatgg cattgttgtt gaacgagatg ggcgccaatg
    12421 atgccgcaga aaaacatctc caactaagtt tggaactagg gcaagccgcc acaataatag
    12481 attggcagta tcgtcgcaac tttgcattgg cacgcttcaa agaaactgct ggcgatttaa
    12541 acgccgcatt gtattttttt gacgaagcca aacgcaccta tgtgaagaca ccgattccgt
    12601 taacacgccc tgtagatgca ttcaaagttc gtgtatatct caagcagggt ttgctcgccc
    12661 aaacacacga gtgggcacag aagttttcaa tggacgatga attaagtttc ttgcacgagt
    12721 ttgagcacat caccttcgcg cgcgtgttgc ttgcgaaaaa agattcaaaa aacgtatcac
    12781 agtttctagc gcgtctctta caggcagctg aggatggcgc acgcattgga agtatgctcg
    12841 agattttgat cttacaagca cttgcgcttc acgcgcaggg acagttgaca caagcactcc
    12901 tcccgctaaa gcgggctgtg gctctcgctg aagcagaagg ctacgtgcgc atctttgaag
    12961 aaggcaagcc catgcaggaa ttgctggcaa agttaagcct tacccctgaa ggca
//
LOCUS       DAOQNJ010000010        12158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_2311_length_12158_cov_8.620838, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000010 DAOQNJ010000000
VERSION     DAOQNJ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2311_length_12158_cov_8.620838"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLG52_00640"
     CDS             complement(<1..202)
                     /locus_tag="PLG52_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB39973.1"
                     /translation="MSLPTQQAILITGASSGIGKHLALRLAERGCIVYATVRKEKDMM
                     ALSEIPNVTPLLMDVRNARHIQS"
     gene            242..883
                     /locus_tag="PLG52_00645"
     CDS             242..883
                     /locus_tag="PLG52_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNB39974.1"
                     /translation="MSRNYVVPLTEIRREQMVVNSRFIATLAPAFSIDEARTFMKRIR
                     EEFADASHNVPVYVIGGGNTVTEYFSDDGEPSGTSGKPALTVLRGSGLGDAVLVVTRY
                     FGGTLLGTGGLVKAYTEAAQSVVNVVERGRRVPVHVALAAIPYNLLERVRLLTTKNGG
                     SILGEEYAADITLTLQFPVEYYTGFQTDLRELTAGKIQAEVIETKEEIIRVDK"
     gene            945..3143
                     /locus_tag="PLG52_00650"
     CDS             945..3143
                     /locus_tag="PLG52_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB39975.1"
                     /translation="MKDKNITIPTIETNRELQGEFFRAVILSIMALTGVFLVFAILIF
                     HELLLRTLIISGGVFFLSVFILFLIKRKKVFLASVFFVGFLWLLVASLTYLTGGVTAP
                     IFAGFLIVILFSGLIFNFRIGVSVALWTVLFGSVLIFAHSKGFLPLAVDYSNGARLAI
                     YTFFFFIALYIQKAAIDSTKLATEKAYISENQYQLFLENIPTITYINDISPEARTLYV
                     SPQVENLLGYSPKTFLQDPALWMKIINEDDVENVLLKNQASVETGEPFKVEYRLMTKS
                     GNVVWVRDEAKLVHDDHGEPAYWLGVWTDITKEKSSEKTLAYMVQTQSMRNNQLQTAS
                     EVSRAATSILELSELMPKVVELIRSHFDYYYVGIFLVEKATNNAILAAATGSAGKEML
                     AKGHSLPIANSSMIGWSILNNRARIALDVGKDAVRFKNPLLPLTRSEVAFPLRFHGRV
                     IGAMTIQSALPSAFTDADITALQTMADQVANAIETARLLDEREALIDELETKNAELER
                     YTYTVSHDLKSPLVTIRGYVGYLKESAVKGDMQRFEVDMKRVIKATETMQALLNDLLE
                     LSRVGRVINSLTNVPFEDMVKDVLTLVVEPQKGQTEWFVVQKGLPTVRCDRTRILEVL
                     QNLILNSIKFMGDQPKPSIRIGQAGFDNKTNFPIFYVADNGIGIEPKYHETVFGLFNR
                     LDQKEEGTGIGLTLVKRIIEVHGGRIWLESEGRGKGTTFYFTLPPAEMKP"
     gene            3258..3749
                     /locus_tag="PLG52_00655"
     CDS             3258..3749
                     /locus_tag="PLG52_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNB39976.1"
                     /translation="MSNSNRTKLLLDIGTFIAFLITMDPRFSGLAIHEWLSLALAGTI
                     IVHLLLNWDWIVNVTRRLFGKANANARVNYVLNWLLFIDGILIMLSGIMISEVAIPSL
                     GLALPMNFAWRRLHDMSANLALLIMGLHLAMHWNWIVTTFKRVFLGGFKKKQPVIAAI
                     EEA"
     gene            3749..4066
                     /locus_tag="PLG52_00660"
     CDS             3749..4066
                     /locus_tag="PLG52_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39977.1"
                     /translation="MMNTLFRVLIILVIATIIGGLMYVGVSASNSSPNGFPEFEGGGE
                     RPPFAEGSEFRPDGDNDGERGERSGFGFPGGIIKAVVLMSVAGGIYSGIVWAGKKTKQ
                     TLA"
     gene            complement(4108..5427)
                     /locus_tag="PLG52_00665"
     CDS             complement(4108..5427)
                     /locus_tag="PLG52_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB39978.1"
                     /translation="MRLSTKLILSFMLISIFSTGIIVVSSRILASREFERFVGDRYTA
                     DLADDLARYYSRNQTWDGVEQEFQHGDHDHHSLYFSIADPQGNIVVAGNEYNVGDPCS
                     QEEFEEGYPIQSNNKTVGVLLLPSAPEQNPLDYEFLRRLNGSIFLSAIATLVFALLIG
                     ILLSRSILRPVRELTKATHHMADGNLGGQVPVRSRDEIGELAQSFNKMSADLARSFNL
                     RKQMTADIAHELRTPISLILGHAEGVKDGVLKPNAENFEIIREEAERLEHLVSDLRTL
                     SLADAGELSVDFQPVDINNFMSDIHAHYLTLFNQKRITLDLETASATLMANLDPNRFA
                     QVLKNILDNALRHTPEGGRVELKTQLTGSSIQLSVKDSGDGVHPEEATHLFDRFYRVD
                     ESRTRDDGGSGLGLAIAKSIVEMHKGRIWAESEKSKGLKVIIEILKI"
     gene            complement(5438..6121)
                     /locus_tag="PLG52_00670"
     CDS             complement(5438..6121)
                     /locus_tag="PLG52_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB39979.1"
                     /translation="MPQKILIVDDERRMVSLLQSYLEQEGYHVLTAYNGREALEVARR
                     ENPDLVVLDIMMPEMNGYEFMNARRAESDTPIIMLTAKVEDEDKIIGLELGADDYVTK
                     PFKPRELMARVRNVLRRAGKNEAKGQTLKVLDITLNRETREVSIGDRHADLTPSEFDL
                     LAALMTAPGKVFSRLDLLDVIQGVRYEGYERTIDTHVKNLRVKVEDDPRKPSHIETVY
                     GVGYRLRKQ"
     gene            complement(6229..6672)
                     /locus_tag="PLG52_00675"
     CDS             complement(6229..6672)
                     /locus_tag="PLG52_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39980.1"
                     /translation="MKTKKWLWWTLTILLTLVVLTGVAIAGFRFGAMHSATLTAEGTS
                     AFAFHHGHDFSGNMTHEGFGMMRGSERGHGFGGRGGFGFFGPLFGLIQLAVFGGLVWL
                     AFSLIKRSGWRLVNVNNSQAAAPAPDVAPEAAEVQVESEEKKDEA"
     gene            complement(6789..9182)
                     /locus_tag="PLG52_00680"
     CDS             complement(6789..9182)
                     /locus_tag="PLG52_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB39981.1"
                     /translation="MIASRWKKVWSDFWSNRGRTFLTIATIAVGTFAVGFNSNLGLYM
                     NESMDGDFLSANPSEAEIYASGLNDDLVKKAREVPGVEAVEGYSSVQGQLIRPDGTKI
                     DISFTAAETPNALTLNFLKPAMGETSIPSYGKKEVILDASASSLGYTAGDTIVIEFDN
                     GKRRELTFTGYMHAVTGFPYNLAQQVNAYVTPDTLAWLGGNINQYGRLSISVSEKQTD
                     ADHVTAVAQAVADELKRAGSTVYFVSVYQPGHHFAYSITQGVFFVLGVLGYLTVLLSG
                     FLIVNTITALMTQHTRQIGIMKATGGSAWQIFFMYLVLIMSFGIGALLLAVPLADIAA
                     QTIGSGMASWLNFYPTDYKGYSITLIQQVIVAILVPLLAAILPIYNSVRITVREALSD
                     YGLGGNVKTKEETVGKNSVLVPRPIRISLRNTFRRKTRLGLTLFTLVLGGAVFIAVYN
                     LWASFDKTIEDIQGYFLADINISFDKGYRYDKVASIATSIPGVEGAEGWLEWTGTLEM
                     NDDEAGTQILFVAPPSNSKLIDPIITSGRWLQPGDENAIVIGNHLLQIFPDLKVGDWL
                     NIDIDGKKTKWQIVGTYSITGNVSPPLLYVNYEYLSVLVGEPEMAYSLRIVTSQHDSA
                     TQNRINDQIQAAFEERGIQISSTQLGADFIRDQKAQTDVLVYFMLVMASLIAFVGGLG
                     LMGTMSINVLERTREIGVMRAIGASNGDIQAIVITEGMVIGILSWAISIFISIPITNV
                     LCYGVGMAILTAPMPAIYGMTGILAWLIFTLVLGTLASALPARRASKLTVRDTLAYE"
     gene            complement(9179..10321)
                     /locus_tag="PLG52_00685"
     CDS             complement(9179..10321)
                     /locus_tag="PLG52_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB39982.1"
                     /translation="MAISFFKPKAEDSDTAERPIIDIRSVNKFYKTAVGDYHALSNVD
                     LQIQAGEFVSIIGKSGSGKTTLLNMITGIDRPSNGEVWVNGTGVHTLNENKMARWRGK
                     NLGVVFQFFQLLPMISVIENIMLPMDFCRMYSPAERRKRAMDLLELVELADHAHKLPT
                     ALSGGQQQRVAIARALANDPPVIIADEPTGNLDSKTAESVFVLFNDLVAKGKTIIIVT
                     HDSAMAKRTHRTALIADGEIVNEYVAKAMPTLSHTQLLQATKSTTPMTFEAGAMILSE
                     GTNADTFYIVSHGTVEVILPRPNQSDVVAVQLGPGKVFGEMEFFHEKKHRASIRASEA
                     GPVEVLAIGYDKLTELLSQSDVTREALREMAGKHNGENLSWRGVSS"
     gene            complement(10367..11488)
                     /locus_tag="PLG52_00690"
     CDS             complement(10367..11488)
                     /locus_tag="PLG52_00690"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB39983.1"
                     /translation="MKSPKLIITILIALALTACGNASAATPESTPIPVVEADDTIIAE
                     GKLEPITYTEISLNTSGLVSELPVEKGERVEAGDVLAVVRSENAQSLEDAQAKATQEL
                     TEAYKEYRDAQSRLDDFDVPARWNGMTPTQAIASSLEKLDVARTEFEPYKHLSEKLIK
                     YEEPKPDFSEMTQEEMEFYEASLENHKNDDRPLNGEARAKKKALDEAWELYRISIRWL
                     ELESNYQNAKVRLANAQADFDALQDTDFSLDTAALRAILANAELRAPHSGVITDLNLK
                     VGEFATDAAPVATIADTSKWLVKTSDLTEIDVVNLEEGQPVTVKLDAIPGKEFKGNVL
                     SIGQNYSENQGDVVYEVTILLSEFDPSMRWGMTAVVTFE"
     gene            complement(11683..>12158)
                     /locus_tag="PLG52_00695"
     CDS             complement(11683..>12158)
                     /locus_tag="PLG52_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNB39984.1"
                     /translation="KNLAEKQTQIIIQFKEPPTTMFPMQTMKPNMLVLYLQPDEGVHL
                     RFEAKAPDTVNETRSVDMEFHYDDAFGKSSIPESYERLLLDALQGDASLFTRADEVET
                     AWSLIDPILQTWETHQTPPLAVYKPGSWGPPEGYDLLARDGRRWLNEEASMMLEK"
BASE COUNT         3130 a         2711 c         2910 g         3407 t
ORIGIN      
        1 ccgattgaat atgcctcgcg ttgcgaacat ccattagcaa tggtgtgaca tttgggattt
       61 cagataacgc catcatgtct ttttcttttc gcactgtggc atatacgata catccacgct
      121 cggcgaggcg tagcgcgagg tgttttccaa taccgctgct ggcaccagta attaaaatcg
      181 cttgttgagt tggaagagac atgataaaaa attccctttt tgatatgagt ataatcaaat
      241 tatgtctaga aattatgtag tgcctttaac tgaaattcgc cgtgagcaga tggtggtcaa
      301 ttcgcgcttc attgctacac tcgcgcctgc tttctccatc gatgaagctc gtaccttcat
      361 gaagcggatt cgagaggagt tcgcagacgc gtctcataac gtccctgttt atgtgattgg
      421 tggtggaaat acggtaacgg aatatttttc agatgatgga gagccaagtg gtacctctgg
      481 caaacctgcg ttgactgttt tgcgtggcag cggattggga gatgcggttt tagtagtcac
      541 ccgttatttt gggggcaccc tgcttggaac tggcgggctg gtcaaggcgt atacagaggc
      601 ggcgcagtct gttgtcaatg ttgtagaacg cggaaggcga gtccctgtgc atgtggcgct
      661 ggcagcgatt ccctataatt tgttggaaag agttagatta ttgaccacca agaatggcgg
      721 tagcatctta ggcgaagagt atgccgcaga tatcacattg actttacagt tccccgtaga
      781 atattacact ggctttcaaa cagatctgcg agaacttacg gctgggaaaa ttcaagcaga
      841 agtcattgaa acaaaagagg aaatcatacg tgtagacaag taagtttttg agttttactc
      901 cctcaataaa aaaacatgta taaagtaaaa ttcaaagtat tgttatgaaa gacaaaaata
      961 ttactattcc gaccattgag actaataggg aactgcaagg ggagtttttt cgtgccgtta
     1021 ttttatctat aatggctttg acaggtgttt ttctggtttt tgccattctt atttttcatg
     1081 aattgttgtt gcggacactt attattagtg gtggggtatt ttttctttct gtttttattc
     1141 tcttcttgat taaaagaaaa aaagtatttc tggcgagtgt tttttttgta ggctttcttt
     1201 ggcttttggt tgcatctctg acctatctta ctggtggagt tactgctcct atttttgctg
     1261 gttttttaat cgttattctt tttagcgggt tgatttttaa cttccgtata ggggtttccg
     1321 ttgctttatg gactgttctt tttggtagtg ttctgatttt tgcacactca aaagggtttt
     1381 taccacttgc cgtggactat tcaaatggcg ctcgattggc gatttataca tttttctttt
     1441 ttattgcgct ttatattcaa aaggcagcaa ttgacagcac aaagctagca acagaaaaag
     1501 cttatattag cgaaaatcaa tatcagctct ttcttgaaaa tatccccacg atcacataca
     1561 tcaatgatat atcgccagaa gcgcgcacac tatatgtcag cccgcaggtc gaaaacttgc
     1621 ttgggtattc tccaaaaacc tttcttcaag acccagcact ttggatgaaa atcattaatg
     1681 aagatgatgt tgaaaatgtt cttttgaaga atcaagcttc tgttgaaaca ggtgagccgt
     1741 ttaaagtgga atatcgtctt atgacaaaaa gcgggaatgt ggtttgggtg cgagatgaag
     1801 ccaaactggt tcatgatgac catggagaac cagcctattg gttgggggtg tggaccgata
     1861 ttaccaagga aaaaagttca gagaaaacat tggcgtatat ggttcaaacg caatccatgc
     1921 ggaataacca gttgcagacg gcgagtgaag tgtcacgagc ggctacctct attttagagt
     1981 tgagcgaatt gatgcccaaa gttgtggagc taatccgctc gcattttgat tactactatg
     2041 tgggtatctt tttagttgaa aaagcgacga acaatgccat acttgctgcg gcaacaggtt
     2101 cggcgggaaa agaaatgctt gcgaaaggac attctttgcc catcgcgaac tcatccatga
     2161 ttggttggtc gattctcaat aatcgggcgc ggatcgcttt ggatgttggt aaagatgcgg
     2221 ttcgtttcaa aaatccgctt ttgccactta cacgttcaga ggtggctttc ccgttgaggt
     2281 ttcatggaag agtcattgga gcgatgacga ttcaatctgc attaccatct gcgtttacag
     2341 atgctgatat caccgcacta caaactatgg cagatcaggt agcaaatgca attgagactg
     2401 cacgtttatt agatgagcgc gaagctctca ttgatgagtt ggaaacaaaa aatgcggaac
     2461 tggaacgata cacttatacc gtatcgcatg atttgaaatc tccacttgta acgatccgcg
     2521 gctatgtggg gtatttaaaa gagtctgctg ttaagggaga tatgcagcgt tttgaagtgg
     2581 atatgaagcg cgtcatcaaa gcgaccgaga caatgcaagc actgctcaac gacttattgg
     2641 aactttccag ggttgggcgt gtaattaatt ctctaacgaa tgtcccattt gaagatatgg
     2701 tgaaggatgt tttgaccctg gtcgttgagc cgcaaaaagg acaaacagag tggtttgttg
     2761 tgcaaaaagg gcttcctaca gtgcgctgtg accggaccag aattttagaa gtgttacaaa
     2821 acctaatctt gaattcaata aaatttatgg gcgatcaacc taaaccctcc attcgcattg
     2881 ggcaagcggg ctttgataat aaaacgaact ttcctatttt ttatgtcgct gataatggaa
     2941 ttggcatcga acccaaatac catgaaacgg tcttcgggtt gttcaatcga ctggaccaaa
     3001 aagaagaggg gacagggatt gggcttactt tagtaaagcg cattattgag gtccacggcg
     3061 ggcggatttg gcttgaatca gaagggcgtg ggaaaggtac tactttttac tttaccctgc
     3121 ctcccgctga aatgaaaccc tagttttaca acttcttcat aatcttttcg catttaatcc
     3181 acctgaaata aaggcatttt catagaatta acaatagtcc tttatataga gactgttaat
     3241 tgaatattgg agagaaaatg tctaactcaa atcgaacaaa attacttttg gatattggta
     3301 cgttcatcgc ctttctcatt accatggacc cacgcttcag tggactggca attcatgagt
     3361 ggctatcact tgccttggct ggcacaatta ttgtccatct cttactgaac tgggattgga
     3421 tcgtaaatgt tacgagacgc ttatttggca aagcgaatgc aaatgcacgt gtcaactatg
     3481 tgttgaattg gttgctgttc atcgatggga ttctcatcat gctgtctggc atcatgatct
     3541 ctgaagttgc catcccgtct ttggggctgg cgttgcctat gaactttgcc tggcgcagat
     3601 tgcacgacat gtctgccaat cttgcgttgc ttattatggg cttgcacctt gctatgcatt
     3661 ggaattggat tgtgaccact tttaaacgag tcttcttagg cggtttcaag aagaaacaac
     3721 cagttattgc cgccattgag gaagcttaat gatgaataca ttatttcggg tattaataat
     3781 tttagtgatc gccacgatca ttggcggatt aatgtatgtg ggtgtgagcg cttcaaattc
     3841 atcacccaat ggctttcctg aatttgaagg cggaggtgaa cgcccgccat tcgctgaagg
     3901 ttccgagttc cgcccagatg gcgacaatga tggagaacgt ggtgagcgca gcggctttgg
     3961 atttcccggt ggcatcatca aagctgtagt attaatgtct gttgctggcg gtatctattc
     4021 aggaattgtt tgggcgggca agaaaacaaa gcaaacgctg gcgtagaaat aatataaaag
     4081 aggacgtgct tagcacgtcc tcttttatta aatcttgagg atttcaataa ttactttcaa
     4141 ccctttgctt ttttcgcttt cagcccaaat acgcccctta tgcatttcta caatagactt
     4201 ggcaatagcg agacccaagc cagagccgcc atcgtcacgc gtgcgggatt catctacgcg
     4261 gtagaaacgg tcaaataggt gagtggcttc ttcaggatga accccatcgc cgctatcttt
     4321 aacggagagc tggatgctgc ttcctgtaag ctgagttttc aattccacgc gtccgccttc
     4381 gggggtgtgg cggagtgcat tgtccagaat attttttagg acttgagcaa aacggttcgg
     4441 gtcgagattc gccatcaggg ttgctgaggc agtttcaagg tccagtgtga tgcgtttttg
     4501 gttgaataga gtcaagtaat gagcgtggat gtcgctcata aagttattga tgtctacagg
     4561 ttgaaaatcc acagaaagtt cacccgcatc ggcgagcgac aaagtccgca gatcgctgac
     4621 aagatgttcg aggcgttcgg cttcttcgcg gatgatctca aaattttcgg cattcggttt
     4681 tagaacacca tcttttacac cttcagcatg accaagaata aggctgatgg gcgtgcgaag
     4741 ttcatgcgca atatcggcgg tcatttgttt acggaggttg aaggagcgcg ccaagtctgc
     4801 actcatctta ttgaacgatt gcgccaattc gccgatctca tctcgcgagc ggacaggaac
     4861 ttgtccgcct agatttccat ctgccatgtg gtgagtggct ttggtcagct cgcgaactgg
     4921 tcgcaaaata ctgcgcgaca gcagaatacc aatcaataat gcaaagacca aagtagcaat
     4981 ggcgcttaag aaaatagagc cgtttaaccg gcgcagaaat tcataatcaa gcggattctg
     5041 ttctggtgca ctaggcaaca aaagcacacc gactgttttg ttgttcgatt gtatggggta
     5101 accctcttca aactcttcct ggctgcacgg atcaccaaca ttgtactcat tacctgcaac
     5161 gactatattg ccttgcgggt ctgcaattga aaaataaagc gaatgatgat cgtggtcacc
     5221 atgttgaaat tcttgttcca caccatccca tgtttgatta cggctgtaat accgagccag
     5281 gtcgtctgcc aggtcagcgg tgtagcgatc gccaacaaag cgttcgaatt cgcggctggc
     5341 aagaatgcgc gaagaaacga caatgatgcc ggtactaaat atgctgataa gcatgaacga
     5401 aaggatgagt ttggttgaaa gacgcatgat tttgaaatta ctgttttcta aggcgatacc
     5461 ctacaccata cacagtttct atatgagaag gttttcgtgg gtcatcttct actttaactc
     5521 ttaagttctt gacgtgagta tcaatcgtgc gctcatatcc ttcgtagcgc acaccttgaa
     5581 tcacatcgag caggtcaagg cgcgaaaaaa cttttccggg ggcggtcatg agcgctgcaa
     5641 gcaggtcaaa ttcagaaggg gttaagtctg cgtggcggtc accaatgctg acttcgcgtg
     5701 tctcacggtt gagtgtgata tcgaggactt tcaacgtttg ccctttggct tcgttcttgc
     5761 cggcacggcg caatacattg cgaacacgag ccatcaactc acgaggcttg aaaggtttgg
     5821 tcacataatc atcggcacca agttcgagtc caatgatttt atcttcatct tccactttcg
     5881 ccgtcagcat aataataggc gtgtcgcttt cggcgcggcg cgcattcata aactcgtaac
     5941 cgttcatttc cggcatcata atatcaagca caacgaggtc tgggttttca cggcgggcta
     6001 cttccaaggc ttcacgcccg ttgtacgccg ttaatacgtg atacccctct tgctcaagat
     6061 aactttgcaa gagagaaacc attcgtcgtt catcgtctac gataagaatt ttctgaggca
     6121 tgaatcgatt atcccataaa ccctgtgaag aaattgtgtg aaatttaata gaacaagagc
     6181 acccgcctca ttccaaggcg ggtgctgctg tggcgttaga gcagtgaatt acgcctcatc
     6241 ctttttctct tcgctttcaa cctgcacttc agcagcttcc ggcgctacgt ctggtgctgg
     6301 ggcagcagct tgtgagttgt tgacattcac aagtcgccat ccgctgcgtt tgatgaggct
     6361 aaatgccaac caaaccaagc cgccgaacac cgcgagttga atcagcccaa acagcggacc
     6421 gaaaaagcca aacccgccac gtccgccgaa accatgtcca cgttcagagc cgcgcatcat
     6481 gccgaagcct tcgtgcgtca tattgccgct gaaatcatgt ccgtgatgga acgcgaaagc
     6541 ggaagttcct tctgcggtga gtgtggcgct atgcatagca ccaaaacgga agcctgcaat
     6601 tgcaacgcct gtcaacacca caagagtcag aaggatggtg agagtccacc aaagccattt
     6661 ttttgttttc atgtttacct ccatttgtaa acttgactct attttttcac acctgtatta
     6721 caaagcaatg gaggaaatgt gtgaaagttg tgtgttcgca gagattgtaa ataacgtacc
     6781 tatagacttt attcgtaagc cagcgtatct ctcacagtca actttgaggc gcggcgtgca
     6841 ggtaacgcac tggcaagagt gcccaacaca agagtgaaga tcagccaggc aaggatgccg
     6901 gtcatgccgt aaatggcagg catgggagcg gtgagaattg ccatgccaac cccatagcac
     6961 aagacatttg tgatgggaat tgaaatgaag atgctaattg cccaactcag gatgccgatt
     7021 accattcctt ctgtgatgac aatcgcctga atatcgccgt tgcttgcgcc aatcgcgcgc
     7081 atcacaccga tttcacgagt gcgttccaac acattgatgc tcatggtgcc catcaagcct
     7141 aaaccaccga cgaaagcgat caatgatgcc atgaccagca tgaaatatac gagaacgtct
     7201 gtctgtgctt tctggtcgcg gatgaaatct gcgcctaatt gcgtggagct gatctgaatg
     7261 ccgcgttctt cgaatgccgc ttgaatctgg tcattgatac ggttttgagt ggcagaatca
     7321 tgttgacttg tgacgatacg gagggagtat gccatttcag gttcgccgac caaaacgctt
     7381 aagtactcat aattcacata taacaatggc gggctgacgt tgccagtgat gctataagtg
     7441 ccgacaattt gccattttgt ctttttaccg tcaatatcaa tgttgagcca atcaccaact
     7501 ttcaggtctg gaaagatttg caagagatga ttgccgatca caatcgcatt ctcatcaccg
     7561 ggttgcaacc agcgaccaga agtgataatg gggtcgatca atttcgagtt agaaggaggc
     7621 gccacaaata atatttgagt tccggcttcg tcgtcattca tttcaagggt gccggtccat
     7681 tccagccatc cctctgcacc ttctactcca ggaatgcttg tggcaatgct ggcgacctta
     7741 tcgtatcgat agcctttatc aaacgaaatg ttgatgtcag ccagaaagta cccttgaata
     7801 tcttcgattg ttttatcaaa ggatgcccac aagttgtaaa cagcaatgaa tacagccccg
     7861 ccaagtacta gtgtgaagag ggttagacct aggcgggttt ttcttcgaaa tgtattgcgc
     7921 aaggaaatgc ggatcgggcg tgggaccaac accgaatttt tgcctacagt ctcttctttt
     7981 gtcttcacgt taccgcctaa accataatcg ctcagtgctt cacgaactgt aattctgacg
     8041 ctgttataga ttggcaggat ggcggctaat aacggaacca agattgcaac gataacttgt
     8101 tgaatcaaag taatggaata gcccttatag tctgttgggt aaaagttcaa ccaagatgcc
     8161 attccactac cgatggtttg ggcagcaata tcggcaagcg gaacagccag caagagcgct
     8221 ccaatgccaa aactcatgat gagcaccaaa tacatgaaaa aaatctgcca ggcacttccg
     8281 ccagttgctt tcatgatgcc gatttgacgt gtgtgttgag tcatcaatgc agtgatggtg
     8341 ttgacgatca aaaatccgct aagcaatacg gtgagatatc ccaaaacgcc caacacgaag
     8401 aacacacctt gtgtaatgct gtaggcaaag tgatgccccg gttgatacac acttacaaaa
     8461 tacaccgtag accctgctcg cttaagctca tcagcaactg cctgagctac agcagtgaca
     8521 tggtctgcgt ctgtctgctt ttcagaaaca ctgatcgaga gtcttccata ttgatttatg
     8581 tttccgccta accacgctaa tgtgtctggc gtaacgtatg catttacttg ttgcgctaag
     8641 ttgtagggga aacccgtcac ggcatgcata tacccagtaa aggtcagctc gcggcgtttg
     8701 ccattatcga actcgataac aatcgtatct cccgctgtat atcccagaga cgaagcgcta
     8761 gcatcaagga tgacttcctt tttgccataa ctgggaatac tcgtttctcc cattgcaggt
     8821 ttgagaaaat ttaatgtcaa cgcattgggg gtttctgccg cagtgaaaga gatatctatt
     8881 ttcgttccat cagggcgaat aagttgcccc tgtacactag aatacccttc cactgcttca
     8941 acaccgggta cttcgcgcgc cttcttgact aagtcatcat ttaagcctga ggcgtaaatc
     9001 tctgcctcag aagggttggc agacagaaaa tccccatcca tactttcatt catgtacaag
     9061 ccaagattgc tattgaaccc tacagcaaac gtcccaacag caattgtggc aatggttaag
     9121 aaggtccgcc cgcggttgct ccaaaaatca gaccagactt ttttccaacg actggcaatc
     9181 atgatgagac tccccgccaa gacagattct cgccattgtg tttgcctgcc atctctctca
     9241 gcgcttcacg cgtaacgtca gactgactca gcaattctgt gagtttgtca tatccgatag
     9301 caagaacctc tacaggtccc gcttccgagg cgcggatgga agcacggtgc ttcttctcgt
     9361 ggaaaaattc catttcaccg aacacttttc ccggtccgag ttgaaccgcc acgacatccg
     9421 attgattcgg gcgcggcaaa ataacttcca ccgttccatg tgagacgatg tagaacgtat
     9481 ctgcatttgt cccttcagaa aggatcatgg cacctgcttc gaaggtcatg ggagtggtag
     9541 attttgttgc ctgtaaaagt tgagtatgag aaagtgtggg cattgccttt gccacgtact
     9601 cgttgacaat ttcgccgtct gcgatgagtg cggtgcgatg ggtgcgtttt gccatggcgc
     9661 tatcatgtgt gacgatgata atggttttgc cttttgcgac aaggtcattg aatagcacaa
     9721 aaacagactc ggctgttttt gagtcaaggt tgcctgttgg ttcatctgca ataatcacgg
     9781 gcggatcatt agcaagcgca cgcgctatcg ctacacgttg ctgttgtcca cctgaaagcg
     9841 cggtggggag tttatgtgcg tggtctgcca gttccaccaa ttcaagcaag tccatggcgc
     9901 gtttgcggcg ttcggcaggg gagtacatgc ggcagaaatc catgggcaac ataatgtttt
     9961 cgatcacaga gatcatcggc aaaagttgaa agaactggaa tacaacgccc agattctttc
    10021 cacgccaacg tgccatttta ttttcattga gcgtatgcac acctgtgcca ttgacccata
    10081 cttcgccatt tgatgggcga tcaatgccgg tgatcatgtt caatagagtt gtcttaccgc
    10141 tgcccgattt gccgataatg ctgacgaact cgcctgcctg aatttgcaga tctacattgc
    10201 tgagcgcgtg ataatcgcca acagcggttt tgtagaactt gttcacactg cgaatatcaa
    10261 tgatcgggcg ttctgcggtg tcgctgtctt ccgctttcgg tttgaagaag gatatagcca
    10321 ttagtaattt ccttcaacac gacatctata tgccgtgttg actactttat tcgaaggtaa
    10381 cgacagcggt catgccccag cgcatagacg ggtcgaactc gctcaaaaga atggtgactt
    10441 catagaccac gtcaccctga ttttcagagt aattctgacc gatggagagt acattgccct
    10501 tgaattcttt gccgggaatg gcatccaact taacggtgac gggctgtcct tcttcaaggt
    10561 tgaccacatc gatctcggta agatcactgg ttttgacgag ccatttggac gtatctgcaa
    10621 tagttgctac gggtgctgca tcagtcgcaa attcaccgac tttgagattc aagtctgtaa
    10681 tgacacctga atgaggggca cgtaattcag cgtttgcaag aatggcacgt aaggcggcgg
    10741 tatcgagtga gaaatctgta tcttgcagag cgtcaaagtc tgcttgagca ttggcaagcc
    10801 gaacctttgc attttgatag ttgctttcca attccagcca gcgaatagag atgcggtata
    10861 actcccaagc ttcatcgagt gcctttttct ttgcgcgcgc ttcgccattc aagggacggt
    10921 catcgttttt atgattttca aggctggctt cgtagaactc catctcttcc tgagtcattt
    10981 cagagaaatc gggtttgggt tcttcatact tgattaattt ctctgaaaga tgcttgtacg
    11041 gctcaaattc tgtacgggca acatccaact tttcgagaga ggaagcaatc gcttgggtag
    11101 gtgtcatacc attccagcga gcagggacat caaaatcatc caagcggctt tgggcgtcgc
    11161 gatattcttt gtaggcttcg gtcagttctt gggtagcttt tgcttgagca tcttcgagag
    11221 attgcgcatt ttcagaacgc acaacagcaa gcacatcgcc tgcttccaca cgttctcctt
    11281 tttccacggg taattcgctg accagtccgc ttgtgtttag ggagatttct gtataggtaa
    11341 taggctccag tttcccttcg gcgataatgg tatcgtccgc ttcaacgacg ggaatgggag
    11401 tgctttctgg cgttgctgct gaggcatttc cgcaggctgt gagagctagt gcaattaaga
    11461 tcgtgatgat aagtttaggc gatttcatgt ccgcttcctt tgaaggattg attatttgtt
    11521 aagacaggat atataaagaa aagtttcaaa tttcagtgaa cttttcgtgt ttacttacga
    11581 cgccatggga agtttggttg cacaaaaaaa gagcacaatc taaaagaatt gtgctctttc
    11641 cgtattactt ttctattctt tttcccaccc ttgagaactg ttttattttt ccaacatcat
    11701 gctagcttct tcattgagcc agcgccgacc atcccgagcg agcaggtcgt agccttcggg
    11761 tggaccccag ctgccgggtt tgtaaacagc caacggcgga gtctgatgcg tctcccacgt
    11821 ctggagaatg gggtcaatca gcgaccaagc tgtttccact tcatctgcgc gggtaaagag
    11881 agacgcgtcg ccttgcagag catcgagcaa aaggcgctca taggattcag gaatggacga
    11941 cttgccaaaa gcatcgtcat agtggaattc catatctacg gagcgggttt cattgaccgt
    12001 atcaggcgcc ttggcttcaa aacgcaagtg gacgccttca tcgggttgca agtaaagcac
    12061 caacatgttc ggcttcatgg tttgcatggg gaacatggtg gtaggcggct ctttaaattg
    12121 gatgatgatc tgggtttgct tttcggcgag gttcttgc
//
LOCUS       DAOQNJ010000011        11698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_2495_length_11698_cov_8.115520, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000011 DAOQNJ010000000
VERSION     DAOQNJ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2495_length_11698_cov_8.115520"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLG52_00700"
     CDS             <1..909
                     /locus_tag="PLG52_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNB39985.1"
                     /translation="IFPVEAVCVDVLQKGMSEIGSLWYENRASVQQEHFASGLAMRRL
                     DALLSASPAPTRAQTILMGCPSNEWHTFTSLLLALLLRRRGLNVIYLGANVPTERFSE
                     TAQKVKANLVILVAQTLNSAAHLQHAALSLSTQKVNVAFGGRIFNLRPSIVDYIPGHY
                     LGATLEGSINEVESLLKGVSKPRKIKAVAQEYVSAMQGFNSKRAHIESTLKLNMPPLS
                     IGTEELDASIQHLGHNVSAALQFGDMEHLSEEMEWLKVLLHAYNRPKQELVYFMESYS
                     NAIDAHINGQGDPIKKWLRSYAVERT"
     gene            914..1699
                     /locus_tag="PLG52_00705"
     CDS             914..1699
                     /locus_tag="PLG52_00705"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNB39986.1"
                     /translation="MNFVSLWLTAGLIILVLMLLLWLYSLAIKNSSIVDIFWGTGFVI
                     TFWATAWIGATALSTRVLLLGVLVTLWGLRLSLYIFSRNHGHPEDFRYAKWREEAGSA
                     WWWRSFFKVFLLQGVIMWVIAIPLIAAQTSTPSPFKCECTDYTGAALWLVGFIFEAGG
                     DWQLARFKKDPANKGKLLTTGLWSLTRHPNYFGDAAQWWGFWLLAFANGAWWTFFSPL
                     IMTYLLMRVSGVTLLENTLTKTKPGYEEYMARTNAFFPWFPKK"
     gene            1782..3059
                     /locus_tag="PLG52_00710"
     CDS             1782..3059
                     /locus_tag="PLG52_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB39987.1"
                     /translation="MNKRLLSIILVVFIDLLGFSLILPLLPYYAEKYGATQFVTGLLV
                     ASYAAMQLIGAPLLGRLSDRYGRRPILLASVFGTFLGFLLLGFADNLGTAMANAFNPQ
                     AANIFVLAILFVSRMVDGLTGGNLSVAQAYISDVTDAENRSKGLGMIGAAFGLGFIIG
                     PATGGILSQYGYAIPGFVAAALSFVNLSLIYFWLPESLTEEKRAEMPDKKPAVTLSAL
                     TKALTRPFTGALLTTRFFYGLAFSIFQTVFALYALQKFNFEARDTGFTLTFVGILSVF
                     VQGFLIGRLTKKFRDDNLILLGGVLMTISLLGWALTPNVFWLLIVLVPTAISGGILNT
                     LLSSTLTKAVQPQEIGGILGLGAAIESATRIFAPIIGGYLLQTFGTWSPGAFGAVMML
                     GITIFIYFTIYNHPIASEIRAKSIQQAPAPNGD"
     gene            3078..3656
                     /locus_tag="PLG52_00715"
     CDS             3078..3656
                     /locus_tag="PLG52_00715"
                     /inference="COORDINATES: protein motif:HMM:NF021577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HNB39988.1"
                     /translation="MNKDILGSFFNRILPLFPPRTEAVLKGMTTSTLEDMNFRYIFTV
                     DGMVTISHIIEEMINKNKGVADLHVSFQYFSRFADQEERYREVADHATGLWLYGVDDA
                     PLPALPRLTAVNTKDTPLENYWFVIAYGPGVSATLLAEEITPDHRLDNEPRMYEGFYT
                     FESDTGYQILMLMHQMFPNQVPLPVAPEKLRS"
     gene            3679..4347
                     /locus_tag="PLG52_00720"
     CDS             3679..4347
                     /locus_tag="PLG52_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB39989.1"
                     /translation="MLIDTLRYELPPEGITLLGYVVRRMHKTEWLVKASEMLANEKTS
                     EALESVAIYAAASSTSFGRAYYQPRFNRVGEIIQERDPITGTSVSAKLESSSPAYHIT
                     YTAALNSGETFNGSETILGTTIGLRGLGMPAPSKFTFKAADYEAEFTGTLTSELALSL
                     FGNTKIRAYGSLDFKDNAGNAGHLDLNRQGYVTLKINDQPTVTHALVRVMWLDMMSLS
                     PQPL"
     gene            4344..5690
                     /locus_tag="PLG52_00725"
     CDS             4344..5690
                     /locus_tag="PLG52_00725"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNB39990.1"
                     /translation="MTNIWWIRRDLRLTDNAALHSALEAGSVLPVFILDPAFDNLSER
                     RKHFLYEGLSALDKDLHKRNSYLVVRKGKPIDALRQLFDEVKAETIYAEEDYTPYSRK
                     RDEFIQKLLPLSLVQGQTVHHPKSVLKQDGRPYTVYTPYSKVWKAQLTKIKLHPALEN
                     LNTPKSIRSEPLPAFTPSPIFPAGEAEALRRLNQFTQSIIKNYHDDRNRMDLDGTSSL
                     SPYIRFGMVGLRQAVHAARATHHADSKGAEIWLNELIWREFYIQILYHFPYVAKTAFN
                     RSLANIPWRNKSSEFEAWKRGETGVPVVDAAMRQLKETGWMHNRARMIVASFLVKDLL
                     IDWRWGEDWFMQNLLDGDIAPNNGGWQWTAGTGTDAAPYFRIFNPVLQSAKFDPNGDY
                     IRKWVPELRGLSAKQIHAPWEYDFKISGYPSEPIVDRDVVKERTLSAYSVSKEKIR"
     gene            5687..6214
                     /locus_tag="PLG52_00730"
     CDS             5687..6214
                     /locus_tag="PLG52_00730"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HNB39991.1"
                     /translation="MNKRDLSVSMERANIIVLFIGIPVVILQFILFDQLHPSPAETGE
                     WNFLLLLLVIVMGVILHEIVHGVTWAIAGRKPWTSIKFGFQAKTLTPYCHITEPLEIN
                     AYRIGAVMPGLVVGILPYIYSLMSGDMNWLWFSLVHTSAAGGDWLVLWLIRHVKAGSL
                     VEDHPSQAGCYVLEG"
     gene            6217..7176
                     /locus_tag="PLG52_00735"
     CDS             6217..7176
                     /locus_tag="PLG52_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB39992.1"
                     /translation="MKKRIQKIAGGFFIVILVILLVGPFLIPVPPLENTVTAESLADE
                     DSQFIEVNGLNVHYKTYGTGEPVFILLHGFGASLFSWHEVTAPLAEYGTVIAYDRPAF
                     GLTERPLEWEGESPYSQDAQVELVIGLMDALGIEKATLVGNSAGGTVSMLTALKYPER
                     IESLILVDPAVYAGGGAPAWIRPLLGTPQMRHVGPLISRQLQAQGVEFIKTAWHDPSK
                     IPQSTLEGYQKPLQVENWDKALWELTLASRESGLAERVNELNLPILVITGDDDRIVPT
                     EQSLRLAEELPNAELAVIPQCGHLPHEECPDDFMQAVKGFLGK"
     gene            7179..8444
                     /locus_tag="PLG52_00740"
     CDS             7179..8444
                     /locus_tag="PLG52_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNB39993.1"
                     /translation="MAQQLSPETGRATLKALFHERSPLGPLKVMAKHVGRFFQIPIPG
                     FSPYVVFGAEAARKVLITDRKKLLWRNTDPVTDLLRRGVLVTDGEEHDHYRSLMETPF
                     HPSQLSKYSEMMIQQVDRVISTWQDGQIVDMLVEGRKIALLIIMQTLFNKDAWNDIPK
                     IWKPILKTIQYVSPGMWILWRKIPRLGYGKSLKVIDDYMYAIIDERRKEERKTNDFLQ
                     HLIDAGLSDDIIRDQMLTMLIAGHDTSTALFAWTFALLGQHPEIHANVAQEVDRAEGK
                     YQLLDNVIKEALRLYPPIHIGNRRVAEEMEFAEGKVPAGERMFYSIYLTHRDPEVWEN
                     AEQFCPERFAHGRKTPPMSYIPFGGGPRACIGAAFGQAEARIVLAHLLKNFTFEFTGH
                     TIHAHMGATLEPRPGVKMKVKRRTVASVQ"
     gene            8510..9304
                     /locus_tag="PLG52_00745"
     CDS             8510..9304
                     /locus_tag="PLG52_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNB39994.1"
                     /translation="MTYFGFLLRFLVIPILVFLVITWWDNKNNRPTFGFNNGKAVWVA
                     ILIHIVLAVVYTTPWDNYLVATGVWYYNPNLVTGIVIGYVPIEEYTFFVLETILAGLW
                     WWFLARRIPEPTNTFKPNKTGRIISFSVLFIFWVIFTYLFFFGDVELTYLSIILFWAF
                     PAIFPQMLYGADILWHYRKLVFITIFVPGLYLSLMDIIALTDTTWSISKEQTTGILFF
                     NILPLEEVLFFFITNVLIGFGMTLLLSKLGTQRFEDWKSNNYKGLP"
     gene            9301..9675
                     /locus_tag="PLG52_00750"
     CDS             9301..9675
                     /locus_tag="PLG52_00750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNB39995.1"
                     /translation="MKNIEQFKKQQYINLETFRKNGGGVKTPVWFAQDGETLRIWTQA
                     DAGKTKRIRRDGKVRIAPSTASGEPIGEWIDANATVLNTQDEINYTKDLFRKKYGVMF
                     NVFGFLGKVRGSSFVTIKVNLN"
     gene            9758..10414
                     /locus_tag="PLG52_00755"
     CDS             9758..10414
                     /locus_tag="PLG52_00755"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNB39996.1"
                     /translation="MINQIEQNILNLLQHLYDELGWFGVTIIMIVENSTGIAPSEVVL
                     ALAGWMLIDAHSLPFSTVFLGGLFAAFGSVMGASILYWAARLGGRPVVERVTHFFRID
                     LRHMQRVERLAEKHGTRFIFFGRLIPGIRMLVAIPAGLTRMPYLTFAIATFLGAYLWC
                     TVFISVGYFLGHEWHLISNIVKQIGPYAFGIAVFFTGIFFIWRRWQRQKLETVKLEIE
                     "
     gene            complement(10451..>11698)
                     /locus_tag="PLG52_00760"
     CDS             complement(10451..>11698)
                     /locus_tag="PLG52_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNB39997.1"
                     /translation="IMVGQLGETSIAALGLSNQVFFVFILLLFGLTSGMAIFTAQFWG
                     KQEVDPIRKVLGMSLAITSAIALLFTLAATLIPRTVLGFYTHDKEVIEIGASYLSIVG
                     FSYIPVAIATSYIATLRSIQLVKLTVIATISALIFKTILGYILIFGVFGLPALGVRGA
                     AIGTASGWTLELILLIILVYAQKTPLAANPLTFFSFDFSFFGRVLKTTLPALANEMFW
                     SLGITTYNAIYARIGTDSIAAINVNATIEELGFVVFMGLGNACAVMVGNRIGAGKKEE
                     AFETVRRVVILALLSAWTVGLIVLFLRDIVVNLYDLSPSGEYNVRMLMLMMACTLWIR
                     MFNFSTFIGALRAGGDTRFALLMELCSIWLIGVPAAYIGAFVLHLPVYFVYLMVALEE
                     VAKAFVSGWRVYSRKWIHDLVNV"
BASE COUNT         3017 a         2940 c         2729 g         3012 t
ORIGIN      
        1 atcttcccag ttgaagccgt ctgcgtggac gtgttgcaaa agggcatgtc tgagatcggc
       61 agcttgtggt acgaaaatcg tgccagtgtg caacaagaac acttcgcttc gggtcttgcc
      121 atgcgccgct tggatgcact tctcagcgcc tcccccgccc ccacccgtgc gcagacaatt
      181 ttgatgggct gcccttctaa tgaatggcat acctttacat cgttactgct tgctttgctt
      241 ttgcgccgcc gcggactgaa cgttatctac cttggtgcta atgtgccaac agagcgcttc
      301 tcggaaaccg cccaaaaggt aaaagccaat ctggtcatct tggtggcgca aacgttgaac
      361 agtgccgcac atcttcaaca cgccgcactt tcgttgagca ctcaaaaggt taatgtagcg
      421 ttcggtgggc ggatttttaa ccttcgcccc agcatcgtgg attacattcc cggtcactac
      481 ctcggcgcta cactagaagg ttcgattaat gaagttgagt ctttgctcaa aggcgtttcg
      541 aagccgcgca agatcaaagc agttgctcag gaatatgtct ctgccatgca aggcttcaat
      601 tccaaacgtg ctcatattga aagcacgctc aaactgaaca tgcctccact ttccattggc
      661 accgaagaac tggatgcgag tattcaacac ttgggtcaca atgtgtctgc tgctttgcaa
      721 ttcggagata tggagcacct ctctgaagaa atggaatggt tgaaagtttt gttgcacgcg
      781 tacaaccgtc ctaaacaaga attagtttat ttcatggaaa gttactcgaa tgccattgac
      841 gctcacatca atggacaggg tgaccccatc aagaaatggc tgcgttcgta cgcagtagaa
      901 agaacctaac tttatgaatt tcgtctccct ttggctgacc gccggtctca ttattcttgt
      961 cctcatgctt ttgctgtggc tgtacagcct ggcaatcaag aactccagca tcgtggacat
     1021 tttctggggc actggcttcg tcatcacctt ttgggcgacg gcatggatag gcgcaactgc
     1081 cctcagcact cgagtcctcc tgctcggtgt gttggtcact ctttgggggc tgcgcctatc
     1141 cctttacatc ttttcgcgca accacggtca ccccgaagac ttccgatatg ccaaatggcg
     1201 agaagaagct gggtcagctt ggtggtggcg cagtttcttc aaagtttttc ttctgcaagg
     1261 cgtcatcatg tgggtcatcg ccatcccatt gatcgctgcg caaacctcca cgccttcgcc
     1321 gttcaaatgc gaatgcactg actataccgg cgctgcatta tggttggtcg gcttcatctt
     1381 cgaagcaggc ggagattggc aactggcacg cttcaagaaa gaccccgcaa acaaaggcaa
     1441 actgctcacc actggcttgt ggagtctcac ccgtcacccc aattactttg gtgatgcggc
     1501 acagtggtgg ggcttctggc tcctcgcctt tgccaacgga gcctggtgga ccttcttcag
     1561 tcccttaata atgacctatc tcttaatgag agtttctggc gtgacattgc tcgaaaacac
     1621 gctgaccaaa accaaacccg gttatgaaga atacatggca cgcaccaatg cattcttccc
     1681 gtggttccct aaaaaataac gtatccaccc tttcaaaacg aaagccataa cgcaaagtcg
     1741 acctttgtgt ggcttcgtgc aacttgaacc aaaaggaaac tatgaacaaa cgcttactct
     1801 ccatcattct ggtcgttttc attgacttgc tcggcttcag tcttatcctt ccgcttttgc
     1861 cctactacgc ggaaaaatac ggcgcgaccc aattcgtgac cggcttactc gtcgcttcat
     1921 acgccgccat gcagttgatc ggcgcaccct tattgggtcg cctctcagac cgttacggac
     1981 gtcgccccat tctgttggca tctgtctttg gaaccttcct cggcttcctc ctgctcggct
     2041 ttgcagacaa tctcggcacc gcaatggcta atgcgttcaa cccgcaagcc gctaacatct
     2101 tcgtgttggc gattctcttc gtcagccgca tggtagatgg gctcactggc gggaacttga
     2161 gcgtggcaca agcctatatc tctgatgtga ccgatgctga gaaccgttcg aaaggtttgg
     2221 gcatgatcgg tgcggcattc ggattgggct tcattatcgg acccgctacc ggcggcatct
     2281 taagccaata tggctacgca attcccggct ttgtagcggc ggcgttgtct ttcgtcaacc
     2341 tctcgttgat ctacttctgg ctacctgaat ccttgaccga agaaaaacgt gctgaaatgc
     2401 ctgataaaaa gccagcggtt acgctttctg cattgaccaa ggcgctgacc cgtccattca
     2461 ccggcgcact tctaacaacg cgcttctttt acgggctggc gttttccatc ttccaaaccg
     2521 tgttcgcgtt atatgcttta caaaaattca acttcgaagc aagagatacc ggttttacac
     2581 tcacgttcgt aggcattttg tctgtttttg ttcaaggatt cctcattggc agactgacca
     2641 agaaattccg tgatgacaac ctaattcttt tgggcggggt tttgatgaca atctcattac
     2701 ttggctgggc attaacaccc aacgtttttt ggctcttaat agttctagtc cccaccgcta
     2761 tttcgggcgg catcctcaat acactcctat cctccactct tacgaaagcc gttcaaccgc
     2821 aagagatcgg cggcattctc gggttgggcg ctgccattga aagtgccaca cgtatctttg
     2881 caccgatcat cggcggatat ttactgcaaa cttttggcac ctggtctcca ggagctttcg
     2941 gggctgtaat gatgttgggc attaccatct ttatttactt caccatctac aaccacccca
     3001 ttgcaagtga gatccgcgca aaatcgatac agcaagcgcc tgcgccaaac ggtgattaat
     3061 tcttaaagga ctttttaatg aataaagata ttcttgggag tttctttaac cgtatcctac
     3121 cgctcttccc tccgcgcaca gaagcggtct tgaaaggcat gacaacatcg acgttggaag
     3181 atatgaactt tcgttatatc ttcaccgtcg atggcatggt gaccatcagc cacatcattg
     3241 aagaaatgat caacaagaac aaaggtgtag ctgacctgca tgtctctttt caatattttt
     3301 cgcgctttgc cgatcaagaa gagcgttacc gtgaagtggc agaccatgct accggtctct
     3361 ggctgtatgg cgtagatgat gctccgcttc ctgctcttcc acgcctgacg gcggtcaaca
     3421 caaaagacac tccgctggaa aactattggt tcgtgatcgc atatggcccc ggcgtaagtg
     3481 caaccttgct cgctgaagaa atcacaccag accatcgctt agacaatgag ccgcgcatgt
     3541 atgagggctt ctacactttt gaatccgata caggctatca gatcctcatg ctcatgcacc
     3601 agatgttccc caatcaagta cctttgcctg tagcgcccga aaaattaagg tcttagtctt
     3661 ttacaaaagg agccattcat gttaatcgac acactacgct atgaattacc gcccgaagga
     3721 attactcttt taggttatgt tgtgcgccgc atgcacaaaa cagagtggct cgtaaaagca
     3781 agtgagatgt tagcgaatga aaaaacaagc gaggcacttg agtccgttgc catttatgca
     3841 gccgcctcct ccacttcttt tgggcgggcg tattatcagc cacggttcaa tcgtgtagga
     3901 gaaatcattc aagaacgcga ccccatcaca ggcacttcgg tatctgccaa acttgaaagc
     3961 agttcacctg cttatcacat tacctacacg gcagccttga acagcggcga aaccttcaat
     4021 ggttctgaaa ccatcctcgg cactactatc gggttgcgcg gtttgggcat gcctgcccca
     4081 tccaagttca ctttcaaagc tgcagactat gaagcagaat tcacaggcac acttaccagt
     4141 gaacttgccc tttccctttt cggcaatacc aagattcgtg cttacggctc gctcgacttc
     4201 aaagataacg cagggaacgc cgggcatctt gacctaaacc gtcagggcta cgtcacacta
     4261 aaaatcaacg atcaacccac cgtcacacac gctcttgtgc gcgtgatgtg gttggatatg
     4321 atgagccttt ctccgcaacc gctatgacaa atatttggtg gattcgccgc gacctacgtt
     4381 tgacagataa tgctgcgctt cattctgcac tcgaagctgg ttctgtcttg ccggtcttta
     4441 ttctcgaccc ggcattcgac aacctatcag aacggcgcaa acattttctg tatgaaggct
     4501 tatctgcttt agacaaagac ctgcataaac gtaattcata tttagtcgtt cgcaagggaa
     4561 aacctatcga tgcactccgt caacttttcg acgaagtaaa agctgaaacc atctatgcgg
     4621 aagaagatta cacaccctat tctcgcaaac gagacgaatt tattcaaaag ctgttgcctt
     4681 tgagtttggt ccaagggcaa accgtacatc accctaaatc ggtcctcaag caagatggta
     4741 gaccttacac ggtgtacaca ccctattcca aagtatggaa agcacagctt accaagatca
     4801 aattacaccc tgcactagaa aatctgaata cacccaaaag cattcgctct gagcccttac
     4861 ctgctttcac gccaagtccc attttcccgg caggcgaagc ggaagcgctc cgccgcctta
     4921 accaatttac tcaatcaatc atcaagaatt accacgacga ccgcaaccgt atggacttgg
     4981 atggaacctc atctctctca ccatacatcc gctttggcat ggttgggttg cgtcaagccg
     5041 tgcatgctgc acgagctaca catcacgcag attctaaagg agcagaaatc tggctcaatg
     5101 aactcatctg gcgtgagttc tacattcaga tcctgtatca cttcccgtat gttgccaaaa
     5161 ccgccttcaa ccgctcactg gcgaacattc cgtggcgcaa taaatcgtca gagtttgaag
     5221 cgtggaaaag gggcgaaaca ggcgtcccgg tggtagatgc ggcgatgcgt cagctcaaag
     5281 aaacgggttg gatgcacaac cgcgcgcgca tgatcgtcgc atcgttcctt gtcaaagact
     5341 tgctcattga ttggcgctgg ggcgaagatt ggttcatgca aaacctgctc gatggcgata
     5401 tcgcacctaa caacggcggc tggcaatgga cggcgggaac gggcaccgac gccgcgccgt
     5461 attttcgtat ttttaacccg gtgctgcaaa gtgcgaagtt cgacccgaac ggtgattaca
     5521 ttcgcaaatg ggtgccagag ttgcgcgggt taagcgcgaa gcaaattcac gcgccgtggg
     5581 aatatgactt caagatatcg ggttatccat ctgaaccgat cgtagatcga gacgtggtaa
     5641 aagagcgcac gttgagcgca tatagtgttt caaaggaaaa aatacgatga acaaacgtga
     5701 tctatctgtt tcgatggaac gcgcaaacat cattgtgctt ttcatcggga ttcctgtagt
     5761 gattctacaa ttcatcctct ttgatcaatt acacccatcg cccgctgaaa ctggtgaatg
     5821 gaatttcttg ttgttgctct tggtcattgt tatgggtgtg atacttcatg agatcgtgca
     5881 tggggttacc tgggcaattg ccgggcgtaa gccttggact tcgatcaagt tcgggtttca
     5941 agccaaaacg cttacaccgt attgccatat aactgagccg cttgagatta atgcgtatcg
     6001 tattggtgcg gtcatgccgg ggttggtcgt tgggattctg ccttacatct atagtcttat
     6061 gagtggagat atgaactggc tatggttcag cctcgtccac acctccgccg ctggtggaga
     6121 ctggcttgtg ctatggctca tccgccacgt aaaggcgggt tctctggtag aagatcatcc
     6181 cagccaggct gggtgttacg tcctcgaagg ataaatatga aaaaacgaat ccaaaaaatt
     6241 gcgggtggat tttttatcgt aatccttgtt atcctattgg ttggtccgtt cctcatccca
     6301 gtaccaccgt tagaaaacac agtgaccgcc gaaagtttgg cagatgaaga tagtcaattc
     6361 atcgaagtca atggtttgaa tgtgcattac aagacttatg gaacgggcga gcccgttttc
     6421 attttgctgc acggttttgg cgcgagtttg ttttcgtggc atgaagtgac agctccgctt
     6481 gcggagtatg gcacggtcat tgcatatgac cgccctgcct ttggtctcac tgagcgcccg
     6541 ctggaatggg aaggtgaaag cccatacagt caggatgcgc aagtagaact ggtcattggt
     6601 ttgatggatg cacttggcat tgagaaggca accttggttg ggaattctgc gggcggaaca
     6661 gtctctatgt tgacggcgtt gaaatacccc gagcgaattg aaagcctcat tctggttgac
     6721 ccagccgtgt acgcaggtgg aggcgcgccc gcatggattc gtccactgct gggcacacca
     6781 cagatgcgtc atgtcggtcc gttgatctca cgtcaacttc aggcgcaagg cgtggaattc
     6841 atcaagactg cctggcatga cccaagtaag attccacagt ccacgcttga ggggtatcaa
     6901 aaaccgttac aagtggaaaa ctgggacaaa gccttatggg agttgacact cgccagccgc
     6961 gaaagcggat tggcagaacg ggttaacgag ttaaatttgc ccattctggt tattactggc
     7021 gatgatgatc gcatcgttcc aacggaacag tctttacgac ttgcagaaga gctacccaat
     7081 gcagaactgg ctgtcatccc tcaatgcggg catttgccgc acgaagaatg cccagacgat
     7141 ttcatgcaag cagtgaaagg ctttcttgga aagtaatcat ggcacagcaa ctttctcctg
     7201 aaaccggtcg cgccactttg aaagcgctct tccacgaacg ctcgccatta ggtccattga
     7261 aagtgatggc aaagcatgtg gggcgcttct ttcagattcc catccccggg ttcagtccat
     7321 atgtcgtctt cggagcggaa gcggcacgca aggtcttaat cactgatcgg aagaagttgc
     7381 tatggcgcaa caccgaccca gtgaccgacc tgctgcggcg cggcgtactg gtgaccgacg
     7441 gcgaagagca tgaccactat cgaagtctga tggaaacgcc attccatccc tcacagttat
     7501 cgaaatattc cgagatgatg attcaacaag tggatcgagt tatatccacc tggcaagatg
     7561 gtcaaatagt ggatatgtta gtggaagggc gaaagatagc tctgctcatc atcatgcaga
     7621 ccttgttcaa caaagatgcc tggaatgaca ttccgaagat ctggaaacca atcctcaaaa
     7681 ccattcaata tgtctcaccg ggaatgtgga ttttatggcg gaagattccg cgtcttgggt
     7741 acgggaagtc gttgaaggtg atcgatgatt atatgtatgc gattatcgat gaaagaagga
     7801 aagaggaaag aaaaacaaat gattttctgc aacatttgat cgatgccggg ttgagtgatg
     7861 acatcatccg cgaccaaatg ctgaccatgc ttattgccgg gcatgatacc tccaccgcct
     7921 tatttgcatg gacctttgcc ttgcttgggc agcatcctga gatccacgca aatgtggcgc
     7981 aggaagttga ccgtgcagaa ggcaaatatc aactattaga taacgtcatc aaagaagcac
     8041 tgcgactgta cccacctatt catattggta accgtcgtgt ggcagaagaa atggaattcg
     8101 cagaaggcaa ggtccctgct ggggagcgta tgttctactc catttacctc acccatcgcg
     8161 accccgaggt ctgggaaaat gctgagcaat tttgccccga gcgtttcgcc catggacgga
     8221 aaactcctcc tatgagttac attccgtttg gcggcggtcc acgggcttgc attggcgcgg
     8281 cattcggtca ggcagaggcg cggatcgtcc tcgctcactt gctcaagaac ttcacattcg
     8341 aattcactgg tcatactatt cacgcgcaca tgggtgccac actggaaccc agaccgggcg
     8401 taaagatgaa agtgaagaga aggactgtag ccagtgtgca gtgaagtatg acttatacaa
     8461 acgactatct gactaacaaa ctattagact aagtgactaa caaactacaa tgacatactt
     8521 tggttttctc ttacgctttc tcgtcattcc catccttgtt tttctcgtca tcacatggtg
     8581 ggataataaa aacaatcgcc ccacatttgg ttttaacaat ggtaaagccg tatgggtcgc
     8641 gattctgatt catatcgtcc ttgccgtggt gtataccacg ccgtgggata actatctagt
     8701 tgccaccggc gtttggtatt acaaccccaa tcttgtgaca ggcattgtca ttggttacgt
     8761 acctattgaa gagtacacct tctttgtgct agaaaccatc ctcgccgggt tatggtggtg
     8821 gttccttgca cgtcgcatcc ctgaaccgac gaacacattc aagcctaaca aaaccgggcg
     8881 catcatctca ttcagtgttc tgtttatttt ctgggtcatc ttcacctatc tgttcttctt
     8941 cggcgatgtg gaattgacct atctctcaat tattctgttc tgggcgtttc ctgccatctt
     9001 cccacaaatg ctgtatggcg ccgatatcct ctggcattac cgcaagttgg ttttcatcac
     9061 gatatttgtc ccaggcttat atctttctct aatggatatt atcgctctca cagacaccac
     9121 ctggtctatt tccaaggaac agaccacagg tattctcttc tttaatattc tgccattaga
     9181 agaagtttta ttctttttca tcaccaacgt cctcattggc tttggcatga cattgcttct
     9241 ttcaaaactt ggcacccagc gctttgagga ttggaaatca aacaactaca aaggtctacc
     9301 atgaagaaca ttgaacaatt caaaaaacaa caatacataa accttgagac gtttcgtaaa
     9361 aatggcggag gagtcaaaac tccagtttgg ttcgcacagg atggagaaac gcttcgtatc
     9421 tggacccaag cggatgccgg caaaaccaag cgcatccgtc gggacggcaa agtgcgcatc
     9481 gcaccatcca cagcttcagg cgaacctatt ggcgaatgga ttgatgccaa tgctacagtt
     9541 cttaatacac aggatgaaat aaactacaca aaagaccttt ttcgcaaaaa gtacggagta
     9601 atgttcaatg tatttggctt tttaggaaaa gtccgcggct caagcttcgt tacgatcaaa
     9661 gtaaatctta actaattttt ctctcattag gattaaacca tggctcatct tttttatgta
     9721 agatagaagc cagtttagat ctagagacaa gaatagaatg atcaatcaaa tagagcagaa
     9781 tatcttaaat ttacttcaac atctatatga tgagctaggt tggtttggcg tcaccatcat
     9841 catgattgtt gaaaactcaa ctggcatcgc ccctagtgag gtcgtgcttg cccttgctgg
     9901 ctggatgttg attgatgcac atagtttgcc tttttctacc gtgtttcttg gagggctttt
     9961 cgccgcattc ggaagtgtaa tgggagcatc gatcttatac tgggcagcac gcctcggcgg
    10021 acgccctgta gtagagcggg ttactcattt ttttcgcatc gacctgcgcc acatgcaacg
    10081 agttgagcgc cttgccgaaa agcatggcac acggtttatt ttctttggca ggctaattcc
    10141 aggcattcga atgttggttg ccattcctgc cgggctgacg cgtatgccct acctcacgtt
    10201 tgcaatagct actttcttgg gtgcatacct ttggtgtaca gtattcatca gtgttggtta
    10261 cttccttggg catgagtggc atcttatcag caatatcgtc aaacagatag gaccttatgc
    10321 ctttggcatt gccgtatttt tcacaggtat tttctttatc tggcggcgat ggcaaagaca
    10381 aaaactagaa acagtaaagc ttgaaattga ataacaaaaa gaggtgctct cggttgagag
    10441 cacctctttt ttagacattc acaaggtcat gtatccattt tcgcgaatag acacgccatc
    10501 cgctcacaaa ggctttcgcg acttcttcca atgcgaccat tagataaaca aaatagactg
    10561 gtaaatgcag aacaaatgcc ccaatgtatg ctgccgggac gccaatgagc cagatggagc
    10621 ataattccat tagaagtgca aagcgggtat caccgccagc ccgcaaggcg ccgataaacg
    10681 tcgagaagtt gaacatacgt atccacaacg tacatgccat catcaacatc agcatgcgga
    10741 cgttatattc gccgctaggt gaaaggtcgt ataagttcac aacaatatca cgcaagaaca
    10801 aaacgattag accgaccgtc catgcagaaa gaagagcaag gatcactact cgtcgaacag
    10861 tttcaaaagc ttcttctttt ttgccagcac caatacggtt cccaaccatg acggcacagg
    10921 cattccccaa ccccatgaat actacgaaac caagctcttc aatcgtcgca ttgacattga
    10981 tagcagcaat agagtctgta ccgatgcggg catagatagc gttataagtt gtaatgccca
    11041 acgaccagaa catttcattg gcaagtgccg gcaaggtggt ctttagaacg cgcccaaaga
    11101 aagaaaagtc gaaagagaaa aaggtcaacg ggttggcggc gagaggagtc ttctgagcgt
    11161 agacaaggat aatgagtagg atcaattcca aggtccagcc agaggcggtg ccaatggcgg
    11221 ctccacgaac cccgagggcg ggcaatccaa aaacgccaaa aatcaaaatg tatccaagga
    11281 tggttttaaa gatcaaagcg gagatagtgg caatcactgt cagtttgacg agttgaatgc
    11341 tgcgcaatgt tgcgatatag gaagtagcaa ttgccacagg aatgtaggag aaaccgacaa
    11401 tactgagata tgaagcgccg atttcaatca cttctttatc gtgtgtataa aagccaagaa
    11461 cagtacgtgg aatcagtgta gcggcaagcg taaacagcaa agcaatggca gatgtgattg
    11521 ccaaactcat gcccaacact ttgcgaattg ggtctacttc ttgcttgccc cagaattgag
    11581 cagtaaaaat tgccataccg ctggtaagac caaaaagaag caaaataaat acgaaaaaga
    11641 cctggttcga caacccaagt gcagcaatgg atgtttcgcc caactgcccc accatgat
//
LOCUS       DAOQNJ010000012        11568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_2529_length_11568_cov_9.219144, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000012 DAOQNJ010000000
VERSION     DAOQNJ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11568
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2529_length_11568_cov_9.219144"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PLG52_00765"
     CDS             complement(<1..1285)
                     /locus_tag="PLG52_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNB39998.1"
                     /translation="MASSLPQKSYAQIPVSLDLPNLIEVQLDSFERLKKEGLGDLFHE
                     VSPIESYNKGMKLFFPSRSPESQQWGLKYWFGEPKHTIEECVERDLTYASPLYVSVLL
                     AGPDVPEPIKQDIFLGDFPEMTDKGTYIVNGTERVVVSQLIRSPGVYFEAPADRATGR
                     LLSMAKLIPDRGAWMEFETRKSDYIILKFNRKRTVPITVFLRALAAVSDGIKDSPIKH
                     GTDEEILSIFKDVDNNPERMFIASTIKQEPDWDLTNHTIAEAALIEFFKKMRPGDPAT
                     LDNARQFLEEQLFDQRHYDLERVGRYKLNQKLDLNIPIPHRTVSKSDIIRLIRRMIQI
                     NNGSERSDDIDHLGNRRVKTVGELIQNKLRIGLRRMERVIKERMSIRDQEQLSPVTLV
                     NIRPVVAALREFFGSSQLSQFMDQTNPLAELRHKRT"
     gene            1493..1565
                     /locus_tag="PLG52_00770"
     tRNA            1493..1565
                     /locus_tag="PLG52_00770"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Phe,seq:gaa)
     gene            1591..1662
                     /locus_tag="PLG52_00775"
     tRNA            1591..1662
                     /locus_tag="PLG52_00775"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1623..1625,aa:Gly,seq:gcc)
     gene            1719..1790
                     /locus_tag="PLG52_00780"
     tRNA            1719..1790
                     /locus_tag="PLG52_00780"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1751..1753,aa:Cys,seq:gca)
     gene            complement(1882..3093)
                     /gene="tyrS"
                     /locus_tag="PLG52_00785"
     CDS             complement(1882..3093)
                     /gene="tyrS"
                     /locus_tag="PLG52_00785"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNB39999.1"
                     /translation="MNIEEQVELLMQGTDYGDEDLQKAMASELRERLKIAEKENRPLR
                     VYCGYDPTSTDLHLGHTISMRKLRQFQDLGHEVTFLIGNYTALVGDPSDKNKARPILT
                     QEQVAENAKTYAEQAFRVLDKSKTKIRYNSEWLSKLSLVDLIRLGQNFTVQQFLAREN
                     FAKRLDKGEPIYLHETFYALMQGYDAAAMETDIQVGGSDQLFNIIVAGRKLQEALGQK
                     PLVGIITGILPGTDGAQRMSKSTGNIVPINTGADDMFGKLMSVPDSAMGTYMRLVTRF
                     NPHEIAQIEKDVASGALHPRDAKMKMAQEIVSIFYTDAEAVSAQDHFIKMFQQKEVPD
                     EMPEYDLQSGQTVVDVILSAGMAESKSKARQLIDQKGVRLDGEVLERGDAAFPHPGVL
                     QVGKRRFLRVK"
     gene            complement(3288..3968)
                     /locus_tag="PLG52_00790"
     CDS             complement(3288..3968)
                     /locus_tag="PLG52_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40000.1"
                     /translation="MKKMSIALLLMVLISACAPAQDSQVVLPQLLPTATAYIDASSYP
                     TAEANVSAAQQTAAGIDVRMNRAWMEGKNVNAEVCFTLPDASDWSIWAANLAYSGIVL
                     QEYGTTLQSIQESANGQPGVRCDILTFVVAPDADLTNAAINIDAIAAPPQPSEYCDLY
                     LPKIQQAMLERGTGVSVDCVDVNGALTMQILNKPAEMSQEQAEQLVYSDEFYTVRGPW
                     SFPFNLSQ"
     gene            complement(3991..>4446)
                     /locus_tag="PLG52_00795"
     CDS             complement(3991..>4446)
                     /locus_tag="PLG52_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HNB40001.1"
                     /translation="TQSVEGLGVRRLVLRTPPVLSRDNVIMKLIALPVQLFVGGPIGS
                     GKQPFPWIHIKDWVSAVRYLMTDESARGVYNMIAPSQTSLADFTKILANVLHRPYWFP
                     VPAFLMRNVLGEMSVLILEGRFSQPKRLLESGYTFQFPGPREALTDLFG"
     assembly_gap    4447..4546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4685..5188
                     /locus_tag="PLG52_00800"
     CDS             4685..5188
                     /locus_tag="PLG52_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNB40002.1"
                     /translation="MNEPVLVLNANFEPIHVCSTRRAVTLILAGKAGMIANGRGHIRT
                     ISQLIPRPSVIRLESQIHRPRPRVKLTRREIFRRDNYTCQYCGKRDTNLTIDHVIPRH
                     MGGKQVWTNLVTACSPCNHRKGGRKVDEAHMHLLHIPKEPPADASYIFGKHLAENQEW
                     TPYITGW"
     gene            complement(5222..5977)
                     /locus_tag="PLG52_00805"
     CDS             complement(5222..5977)
                     /locus_tag="PLG52_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB40003.1"
                     /translation="MAKVIILGASNAIPTSESENTHMVIVGNDRTVLIDSVSNPILRL
                     EQAGLDFNNLTDIIITHFHPDHASGIPLLLMDMWLMGRQKPLNIYGLHYTLDRVEGLM
                     GFYNWSEWPNFFPVVFYRLPVREMTPVLDCPDFIIHASPVHHMIPNIGLRVNFQQSQK
                     VMAYSCDTEPCDEVVRLSEGADVLIHEATGESPGHSSATQAGEIASRAEVNKLYLIHY
                     PTGRFKKGDIAAEASQTYQGEIQIAKDFMVLDF"
     gene            complement(6079..7512)
                     /locus_tag="PLG52_00810"
     CDS             complement(6079..7512)
                     /locus_tag="PLG52_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNB40004.1"
                     /translation="MFNKLRPVWLVAQRELRDQFRDWRVLIPMAILTFCFPFLMNEFA
                     KQTVDFLNQYNANLILDRLVPFSIMIIGFFPITVSLVVALESFVGEKERGTIEPLLTA
                     PFDDWQLYFGKLLVGAATPLIASYVSIGLYMLLIIRQDLTMPSASVMVQLLLLTGAHA
                     VLMVSAAIVISVQSTSVKAANLLASFIVIPVAILMQGEAVMLFWGNETVLWLGVAGVL
                     IISLLLIRVGIAHFEREYLLGREIDNLNFKWMLRTFSKNFTGGAASVGDWYRRVVASS
                     FRRALPAMGWMLLVAVVSVWMSYNWVTTNVSQAITSASPDTLAKLEKGIENLPDLSSL
                     GGQLSAPFLFFNNTRSMTVIFLAGIFSFSVLGVLLYMVNIALIGGVYAVLELLGIAPA
                     PIFLAGILPHGIFELPALLIGSAVVLYIGAALVTPQNGKSMGEVMIDLMADWAKIFVG
                     VVVPLLAIAALIEAYITPSILLRVVGN"
     gene            complement(7502..8437)
                     /locus_tag="PLG52_00815"
     CDS             complement(7502..8437)
                     /locus_tag="PLG52_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40005.1"
                     /translation="MIETNDLSKQFHDFMAVDGVNLSIGAGQILALLGQNGAGKTTTV
                     RMLTALLQPSRGSARVAGYDVTKNGHDVRASVGVLTEHHGLYMRMTGSEYMDFFGQVY
                     NLDPSLRKSRTENLLEYFGLAEASKRRIGEYSKGMRQKLALARAMMHEPSVLLLDEPT
                     SAMDPESARLVRDEIARLKSTQRTIMLCSHNLTEVELLADKIAIIYRGKILIQGSLDE
                     LKSGVLGAPEYVIKFASAWDASRLEMPKGMELLEKTATSLKVRVEAPAESNPELIQRL
                     TSLSAPVVAFQEETRTLEQVYLKVMATAKGKEYVQ"
     gene            complement(8515..9315)
                     /gene="lgt"
                     /locus_tag="PLG52_00820"
     CDS             complement(8515..9315)
                     /gene="lgt"
                     /locus_tag="PLG52_00820"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNB40006.1"
                     /translation="MTDGLNLGFLLIRWNGLLIALGIAVGALVAAIEAKRRFQDAELI
                     YYLFVPVTVWSWIGARLWHVFTPPLSSVQLGLTTSHYLSHPLDILSVWTGGFGIPGAI
                     LGGAFALWLASRKYELSFWEVADVLALGMVLAQAIGRIGNYFNQELYGLPTNLPWSIF
                     IQPDHRLLSYEQVEFYHPLFAYEAIFVFLGFVLLFWLSRSKFADTFFAGDIYLGSLAF
                     YSLVRFLLENLRLDVALVNGVNANQLFFAILFIVSISILGHRRLAPVL"
     gene            complement(9312..9941)
                     /locus_tag="PLG52_00825"
     CDS             complement(9312..9941)
                     /locus_tag="PLG52_00825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNB40007.1"
                     /translation="MNTKPTFSDRARIIFKGILDPIGNFLNGLGLTPNTITLLGLAGT
                     TVGAYVISQGHMTTGAFILLASVLVDAFDGTMARLRGEPSDFGGFVDSVSDRYAELVT
                     FGGLVYYFLTKADYPGVMISFFAAAGSVLVSYVKARAEGLGFTAKVGILTRLERYIVL
                     IPLLVFNQPFAAVIIIAVLANITAFQRILNVRAQGRERIKKSKESKEAK"
     gene            complement(9954..>11568)
                     /locus_tag="PLG52_00830"
     CDS             complement(9954..>11568)
                     /locus_tag="PLG52_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNB40008.1"
                     /translation="EPEVPQEIAVEQPVSKEAAAPKKKAPAIPPEFRTQLKSAEKKPE
                     KSLKPPPRGEDLPPLTVLMGDSTVRTDERTINQTAGLIMKTLSEFGIPATVIGYRVGP
                     SVTQFAVQPGFIKKGNEEEDAQQMKVRVAQIASLEKDLALALSAQRLRIEAPVPGKPY
                     VGIEVPNVNSSIVRMRALLESDAFYKVGAPLTIALGRDVSGNPLMADLSRMPHLLIAG
                     STGSGKSVCVTAIAACLAMNNTPEELRIVMIDAKMVELIRFNGLPHLYGKVETNLERI
                     LGVLRWVVVEMEHRYRLLESMHARDLNSYNKKVSRSADGEQLPRIVVIIDELADLMMS
                     APDQTEHNLVRLAQMARATGIHLVVATQRPSTDVVTGLIKANFPARLAFAVASGTDSR
                     VILDYTGAESLLGRGDMLFLNPEVGTPVRAQGVLITDTEIERIIAYWQKNWDTDAVEV
                     PPWETLLKEPEEDEDDGLVEKAISIVRQQQRASASMLQRRLRIGYPRAARLLDQLETM
                     GVVGPSQGGGKEREVLISDEDEIGNQEER"
BASE COUNT         3156 a         2820 c         2667 g         2825 t
ORIGIN      
        1 gtgtacgctt atggcgtaac tcagccaatg ggttggtctg atccatgaat tgggaaagct
       61 gggaagaacc gaagaactcg cgcaacgcag ccaccactgg gcggatattg acgagagtta
      121 caggtgaaag ctgttcctga tcgcgaatgg acatgcgttc tttaataacg cgctccatac
      181 ggcgcaaacc gatgcggagt ttgttttgga tcaactcacc cacggtcttc acgcggcggt
      241 tgccaaggtg atcgatatca tctgaacgct cagagccgtt attgatttgg atcatacggc
      301 gaattaagcg gatgatgtcg cttttggaaa cggtgcgatg aggaatcggg atgttgaggt
      361 cgagtttttg attcaactta tagcgaccaa cgcgttcaag gtcatagtgg cgctgatcga
      421 agagttgctc ttcaaggaac tggcgggcgt tatccaacgt agccgggtcg ccagggcgca
      481 ttttcttgaa gaattcaatc aatgcggctt cagcaatggt gtggttggtc aggtcccaat
      541 ccggttcctg tttaatagta gaggcaatga acatgcgctc tgggttgtta tctacatctt
      601 taaagatgga aaggatttct tcatcggtac cgtgcttgat gggagaatcc ttgatgccat
      661 cgctaaccgc tgccaaagcg cgtaagaaca ctgtgatggg cacagtgcgt ttgcggttga
      721 acttgaggat gatgtaatca gacttacggg tttcgaactc catccaagcc ccacggtcag
      781 gaatcagctt ggccatggag agcaggcgtc ccgtagcgcg atccgcgggg gcttcgaagt
      841 acacaccagg agaacggatc aactgcgata caacgacgcg ttccgttcca ttgacaatgt
      901 aggtgccctt gtcagtcatc tcggggaagt caccgaggaa gatgtcctgt ttgatgggtt
      961 cagggacatc gggaccagcc aaaagcacgg aaacatacaa cgggctggca taggtcaggt
     1021 cacgttctac acattcttca atggtatgct taggttcacc aaaccaatat ttcaaccccc
     1081 actgttgcga ttcggggctg cggctcggga aaaataactt cattcccttg ttatatgact
     1141 caatagggga aacttcatgg aaaaggtctc caagtccctc tttcttgagc ctttcaaatg
     1201 aatccagttg aacttcaatc aggttgggta aatcaagact tacaggaatt tgtgcgtagc
     1261 ttttctgggg caaagaagat gccattcttc tctcctaacg gttttgaatg actatataaa
     1321 cgatgagaac tgtcccggat ggacagcgac tagacattat agcgaatgga atataaaaag
     1381 tcaagaaatt ttcattttca tttattctct tttatattgt tcgttgtttt ttcttgacaa
     1441 cataaataag tacgtgtaaa atcacccgcg cttaacaaaa gaataatgtt tggccgagat
     1501 agctcagttg gtagagcacg cgactgaaaa tcgcggtgtc cccagttcga tcctgggtct
     1561 cggcactctc gaccaaatcg gtcgatacac gcgggcgtag ttcagtggta gaacgtctcc
     1621 ttgccaagga gaaggtcgtg agttcgaatc tcatcgcccg ctcaactgga ggattgcaga
     1681 ttgaaaatgc tttcggtcag caatcctttt gtttataagg cgtcgtggcc aagtggtaag
     1741 gcaagggtct gcaaaaccct cattcacggg ttcaaatccc gtcgacgcct caaaaacaga
     1801 ctcaaccttg agtctgtttt ttgttttatc aaatagtttc ttaaagaaaa aggacttgca
     1861 tgtgcaagtc ctttttatgt tttatttgac acgcaagaag cgacgcttcc caacttgcaa
     1921 cacgcctggg tgtggaaacg cagcatcacc acgctctagc acttccccat cgagacgaac
     1981 tcccttttgg tcaatcaact gcctcgcttt gcttttgctt tctgccatac cagcagaaag
     2041 gatcacatca acaacagttt gccccgattg caaatcatat tctggcattt cgtccgggac
     2101 ttccttttgt tggaacatct tgatgaaatg gtcttgagcc gatacagctt cagcatccgt
     2161 atagaagatg ctgacgattt cttgtgccat cttcatcttt gcatcacgcg gatgtaatgc
     2221 acctgaagca acatcttttt cgatctgggc aatctcatgc ggattgaaac gagtcacgag
     2281 acgcatatag gttcccatcg ccgaatcagg gacggacatt aatttgccga acatatcgtc
     2341 tgcaccggta ttgataggca caatattgcc cgtagactta gacattcttt gtgcgccatc
     2401 ggtgcccggc aaaatgccgg tgatgatgcc aaccagaggt ttttgcccca aggcttcttg
     2461 aagtttgcgc ccagccacga tgatattgaa caactgatcg gaaccaccaa cttgaatatc
     2521 cgtctccatg gcggcggcat cgtatccttg cattagggcg tagaaagtct cgtgcaggta
     2581 aatgggttcg cccttatcga gacgcttggc aaagttctca cgtgccaaga actgctgaac
     2641 cgtaaagttt tgaccaagac ggatcaaatc gaccaagctg agtttggaga gccattcact
     2701 gttatagcgg atttttgttt tagatttatc caatacgcga aatgcctgtt cggcgtaggt
     2761 cttggcattc tcggcgactt gttcctgcgt cagaatcgga cgcgctttat ttttgtccga
     2821 agggtcgccc accaaagctg tgtagttacc tatcaggaag gtcacttcat gacctaaatc
     2881 ctgaaactga cgcaatttgc gcatggaaat ggtatgaccc aaatgcaaat ctgtagacgt
     2941 tggatcataa ccacaataca cccgcaaagg acgattttct ttttcagcaa tctttagacg
     3001 ctcacgcagt tccgatgcca tggctttttg caaatcttcg tcgccatagt cggttccctg
     3061 catcaataac tcgacttgtt cctcgatgtt cattcttttc tcctaatgca aaccctgttt
     3121 gggtcgtaat tttttatatc atttgagcgc tatacaaaca aaaaacgcgc ccgcgatgat
     3181 ggggcgcgtt caaatcacac catcgcaacc agttacgggt ggtaataata gtaatagcga
     3241 ctagtgaata agttgccaga catgctcggt attatacatc aggaatttta ttgcgacaaa
     3301 ttgaaaggga agctccacgg tccgcgtacg gtgtagaact catcgctata cacaagttgt
     3361 tctgcttgtt cctgtgacat ctcagcgggc ttgtttagga tttgcattgt caaagcaccg
     3421 ttaacgtcca cacagtccac agagacgccc gttccgcgct ccagcatggc ttgttgaatc
     3481 tttggcagat acaaatcaca atattcgctg ggttgtgggg gggctgcaat tgcatcgata
     3541 ttgatggcgg cattggtcag gtctgcatcg ggagcaacaa caaaggtcaa aatatcacaa
     3601 cgcacaccag gctgaccatt tgctgattcc tgaatgctct gcaatgtggt tccgtattct
     3661 tgcagcacaa tgccactgta agctaaattc gccgcccaaa tcgaccagtc tgacgcatcg
     3721 ggcaaggtaa agcaaacttc tgcattgaca ttctttcctt ccatccatgc acggttcatg
     3781 cgcacatcga tccctgctgc ggtttgttga gcagcgctta catttgcttc tgctgttggg
     3841 tatgacgatg catcaatata agctgtggca gttggcaata actgaggcaa caccacctgc
     3901 gaatcttgtg caggggcaca agcggatatc agcaccatta acagcaaagc aatagacatc
     3961 ttcttcatgg acttttttat ctcctttgac ttaaccaaat aaatcggtca acgcctctcg
     4021 cggacccgga aactggaacg tgtacccaga ctcaagcaac cgtttaggct gggaaaacct
     4081 tccttcaagg atcaagacgc tcatttcacc caaaacgttc cgcattaaaa aggctgggac
     4141 gggaaaccaa tatgggcgat gcaatacatt ggctaaaatc ttggtgaaat cagccaatga
     4201 ggtctgcgaa ggcgcaatca tattatagac gccacgtgcg ctttcatccg tcatcaaata
     4261 gcggacagca ctcacccaat ctttgatatg aatccaaggg aagggttgtt tgccagaacc
     4321 aatgggtccg ccaacgaata attgcactgg caaagcaatc aatttcatta ttacattatc
     4381 tcgggaaagt acaggtggtg tacgcaaaac caacctgcga acgcctaatc cttcaacaga
     4441 ttgagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttt gtcagggcgg
     4561 tgccaagcaa gcctgtgccg ccagagatca tgatattcat ttttgctcct gcaatgatat
     4621 gtcaatgggt gtttaaactg atttaacatt ctagatgtat aatcacgcga gttaccggaa
     4681 ccttatgaac gaacccgtac tggtgctcaa cgcaaatttt gagccgatcc acgtctgttc
     4741 gacacgacgg gcagttactt tgatccttgc gggaaaagcc ggtatgatcg caaacggacg
     4801 cggacacatc cgcaccatat cacagctcat ccctcgtcct tcggttattc ggctggaatc
     4861 gcaaatccat cgaccacgcc cacgcgtcaa attaacgcgc cgcgagatct ttaggcgcga
     4921 taattatacc tgccagtatt gcggcaaacg cgacacgaac ttgacgatcg atcatgtgat
     4981 tccgcgccat atgggtggta aacaagtttg gacgaatttg gtaacggctt gttcgccttg
     5041 taatcatcgc aaaggcgggc gcaaagtgga tgaagcacac atgcatttgc tccatatccc
     5101 caaagaacca cctgccgacg cttcttatat ttttgggaaa cacttggcag aaaaccaaga
     5161 atggacacct tatattaccg gttggtaaat gaaaatcccc tcatcacgaa gggatttttt
     5221 attagaagtc tagcaccata aagtcttttg ctatctggat ctcgccctga taggtctgag
     5281 atgcttcggc ggcaatatcg ccttttttga atctgccggt gggatagtga atgagataca
     5341 gtttatttac ttctgcccgc gaagcgattt ctccggcttg agtggcagag gagtgaccgg
     5401 gcgattcgcc cgtcgcttcg tggatgagca cgtctgcgcc ttcgcttaag cgaacaactt
     5461 cgtcacaagg ttcagtgtca catgaatatg ccattacttt ttggctttgc tggaaattga
     5521 cgcgtaaacc aatgttggga atcatatggt ggactggaga ggcatgaata ataaagtcgg
     5581 gacaatccaa cacgggggtc atctcgcgga cgggtaaacg atagaaaacg accgggaaaa
     5641 agtttggcca ctcagaccag ttataaaagc ccatcaagcc ttcaacacgg tcaagagtgt
     5701 agtgcagacc atagatattg agcggttttt gcctgcccat cagccacata tccataagca
     5761 aaagcggaat accggaggca tggtcgggat gaaaatgggt gatgataatg tcggtaaggt
     5821 tattaaagtc gagcccggct tgctcaagcc gtagaatggg attgctcact gaatcgatca
     5881 gaacggtacg gtcatttccc acaatgacca tgtgtgtgtt ttcgctttcg cttgtgggaa
     5941 ttgcgtttga agccccaagg atgatgactt ttgccataat caaatcttaa ctcaaatccc
     6001 cttaattgtt aattgtattt tcatatgagc cataagaaaa gcgagctgcc ttgtataaaa
     6061 cagctcgctt ttcttgaatt aatttccgac aactcttaat agaatactag gggtgatata
     6121 tgcctcaatt aaggctgcga ttgcaagcaa tggcaccacc acaccaacaa atatctttgc
     6181 ccaatctgcc atgagatcga tcataacctc ccccatcgac ttgccatttt gcggtgtgac
     6241 caaggcagca ccaatataca acactacggc actaccaatt agcaaagccg ggagttcaaa
     6301 aataccatgc ggcaaaattc cagccagaaa gatcggagca ggtgcaatgc ctaaaagttc
     6361 caaaacggca tatacgccgc caatcaaagc aatgttgacc atatacagca gaacgcccaa
     6421 gacactaaac gaaaatatcc cagcgagaaa tataacagtc atagaacggg tgttgttgaa
     6481 aaacaagaag ggcgcgctta attgtccacc caggctggaa aggtctggta ggttttcaat
     6541 acccttttca agctttgcca acgtatcggg cgaggcgctg gtaatcgctt gagatacatt
     6601 ggttgtaacc cagttgtagc tcatccatac actgacaacc gccaccaaaa gcatccaacc
     6661 catggcgggc aatgctcgcc gaaaagaaga agcaacaacg cggcgatacc aatctccgac
     6721 agatgctgcg ccaccggtaa agtttttcga gaaagttcgt aacatccact tgaagttgag
     6781 attatcaatc tctcgtccaa gtaaatattc acgctcaaag tgcgcaatcc ctacccgaat
     6841 caatagcaaa gagataatca acacaccggc aacgcccagc catagtacag tctcattgcc
     6901 ccaaaacaac atcacagctt cgccttgcat cagaatcgcc actggaatca caatgaacga
     6961 tgctaacaaa tttgccgctt tcactgatgt ggattgcacc gaaataacaa tcgcggcact
     7021 aaccatcaag accgcatgtg ccccagtaag caaaagcaat tgcaccatca cagaggcgct
     7081 aggcatggtt aagtcttgac gaatgatcaa caacatatac aagccaatgg aaacatagct
     7141 ggcgatcaac ggcgtagcag ccccaaccag caacttacca aaatatagtt gccaatcatc
     7201 aaatggcgcg gtcaacaaag gctcgatcgt gccacgttct ttctcgccca caaaagactc
     7261 taatgccacc acgagtgaaa cagtaatagg aaagaatcct atgatcatga tagagaacgg
     7321 aaccagacga tctaatatca ggttggcgtt gtattggttc aagaaatcca cggtctgttt
     7381 tgcaaattca ttcatcaaga atggaaaaca gaaagtcaga atcgccatgg gaatcaaaac
     7441 gcgccaatcg cggaactggt ctcgcagctc acgttgggct acaagccaaa caggtcttaa
     7501 tttattgaac atattctttt ccctttgcag ttgccatcac cttcaagtag acctgttcca
     7561 gtgtgcgcgt ttcttcctga aaggcaacta caggcgcgga caaggaagtg agccgctgaa
     7621 tcaattctgg atttgactct gctggcgctt ccacccggac tttcaaactg gtcgctgtct
     7681 tctcgagtaa ttccatccct ttgggcattt ccaacctact cgcatcccaa gcagatgcaa
     7741 atttgatgac atattcagga gcccccaaaa cacctgactt caactcatct aatgagcctt
     7801 gaatcaaaat ctttccacgg taaataattg cgatcttatc tgcaagaagt tccacttcgg
     7861 tgaggttgtg agagcacaac atgatggtcc tttgcgtaga cttgagcctc gcaatttcat
     7921 ctctcaccaa ccgcgcggac tcggggtcca ttgcagaggt gggctcatcc aaaagtaaga
     7981 cgctgggttc gtgcatcatt gcgcgagcaa gcgctaattt ttggcgcatc cccttggaat
     8041 attccccgat acggcgtttg gaggcttctg ccaagccaaa gtattcaagc aggttttcgg
     8101 tacgtgattt acggagggaa gggtcgaggt tgtagacttg cccaaaaaaa tccatgtact
     8161 ctgaaccggt catacgcatg tacaaaccgt gatgttcagt gagcacgccc accgaagcgc
     8221 ggacgtcatg cccgtttttt gtcacatcgt agccagctac acgtgcagag ccgcggcttg
     8281 gctgcaataa ggcagtcaac atacgcacgg tcgttgtttt gcctgcgcca ttttgcccca
     8341 acaaggcaag gatctgcccg gcaccaatgg agagatttac tccatcgaca gccataaaat
     8401 catggaattg tttactaaga tcgttggtct caatcatgag gtatcctttt ctactaagaa
     8461 agaggctacg cccattttat tacccatccc ccacggcttt ccttctcaaa tcccttacaa
     8521 aactggtgca agccttctat gccccaggat gcttatagag acaatgaaaa gaatagcaaa
     8581 aaatagttga tttgcattga cgccattgac caatgccacg tcgaggcgaa ggttctcaag
     8641 cagaaaacga actaaggaat agaatgctaa agaaccgaga tagatatcac cagcaaagaa
     8701 ggtatccgca aatttgctgc gagaaagcca aaataacaag acaaagccca gaaacacaaa
     8761 aatggcttca taagcaaata agggatggta aaactcgact tgttcgtaac tgagcaagcg
     8821 atggtctggc tgaataaaaa tcgaccaagg caaattcgtt ggcaagccgt agagttcttg
     8881 attgaaataa ttgccaatac gccctatggc ttgcgccaat accatgccca gagcaaggac
     8941 atctgccacc tcccaaaaag agagctcata tttacgagat gccaaccaca aagcaaaagc
     9001 tccgccaagg atggcgcccg gaatgccaaa gccgcctgtc catacagaga gaatgtctaa
     9061 gggatgagag agatagtgac tggtggtaag accaagctga acggaggaaa gaggcggagt
     9121 gaacacatgc caaagcctgg caccaatcca tgaccacacc gtcacaggga cgaagaggta
     9181 gtagataagc tccgcatctt gaaagcggcg tttggcttca atggctgcga ccaaggcacc
     9241 aaccgcaatg cccaaagcga tcaataaccc gttccagcgg atcagtaaaa agccgaggtt
     9301 taagccgtct gtcacttggc ttccttggat tcttttgatt tcttaatgcg ttcccgccct
     9361 tgtgcgcgga cattcaagat gcgttggaaa gcagtaatat ttgccaatac tgcaatgata
     9421 atcaccgcgg cgaacggctg gttgaaaacc agtaaaggaa tcaacacaat atatcgttcg
     9481 aggcgcgtga gaattccaac tttagcagta aagcccaagc cttcagcgcg ggctttgacg
     9541 taggagacca aaaccgaacc tgccgcagca aagaaggaga tcatcacacc cgggtagtca
     9601 gcttttgtta gaaagtaata gaccaaaccg ccaaaagtga ccaactctgc atagcgatca
     9661 ctgaccgaat caacaaaacc gccaaaatca ctgggttcac cacgcaaacg tgccatcgtt
     9721 ccatcaaagg catcgactag tacagacgcc agcaaaatga aagcgccggt tgtcatatgc
     9781 ccttgcgaaa tgacataggc gccaaccgtt gtccctgcca aacctaataa tgtgatggtg
     9841 ttcggggtaa gcccaagccc gttgaggaaa ttcccaatcg ggtcgagaat acctttaaat
     9901 atgatacggg cacggtctga gaaagttggt tttgtattca tgtctacgta tttttatcgt
     9961 tcttcttggt tacctatttc atcttcgtca ctgataagca cttcacgttc tttgcctccg
    10021 ccttgagacg ggccaacgac acccattgtt tctaattggt ccaacaaacg agccgcgcgt
    10081 gggtagccaa tgcgcaagcg acgttgaagc atggaagcag aagcacgttg ttgttggcga
    10141 acaatggaaa tggctttttc caccaaacca tcgtcctcat cttcttccgg ttctttcaac
    10201 aatgtttccc aaggtggaac ttcaaccgca tcagtatccc aattcttttg ccaatacgca
    10261 atgatacgtt cgatctcagt atctgtgatc aatacgccct gtgcgcggac aggtgttccc
    10321 acttctgggt tgaggaagag catatcaccg cgaccaagca aagactcggc gccggtgtaa
    10381 tcgagaataa cgcggctatc ggtgcccgat gcaactgcaa aggcaagacg cgctgggaag
    10441 ttcgctttga taagacccgt cactacatcg gtggatggac gttgggtcgc aaccacaagg
    10501 tgaattcctg tagcacgtgc catttgtgcc aagcggacga ggttatgttc ggtctggtct
    10561 ggcgcagaca tcatcaagtc tgcgagttca tcgatgatga ctacaatgcg cgggagttgt
    10621 tccccatcag cagaacgtga gaccttcttg ttgtacgaat tcaggtcacg ggcgtgcatc
    10681 gactcaagca agcgataacg atgctccatt tccacgacca cccaacgcaa cacaccaagg
    10741 atgcgttcaa gattcgtctc tacttttccg tacagatgtg gcaagccatt gaaacggatc
    10801 aattccacca tcttggcatc gatcatcaca atgcgcagtt cttcgggcgt gttgttcatg
    10861 gcaagacaag ccgcaattgc agtaacacac accgatttac cagatcccgt agaacctgcg
    10921 atcaataagt gcggcatacg agataaatct gccatcaacg ggttgccaga gacatcacgt
    10981 cccagggcaa tggtcaacgg agcgccaact ttatagaaag catcgctttc caacaaggct
    11041 cgcatgcgga cgatagaact gttgacgttc gggacttcga taccaacata aggtttaccc
    11101 ggaactggcg cctcaatacg tagacgttga gcggatagcg ccaacgcaag gtctttctcc
    11161 agtgaagcga tttgcgccac acgaaccttc atctgttggg catcttcttc ttcattacct
    11221 tttttgatga agcccggctg aacagcgaac tgcgtcacag acggacccac acgatatcca
    11281 atcaccgttg caggaatacc aaattcggaa agggtcttca tgatcagacc agcagtttgg
    11341 ttgatcgtgc gctcatcggt gcggacggtt gagtcgccca tgagaactgt caatggcggc
    11401 aaatcttcac cgcgtggtgg cggcttgaga gacttctcag gcttcttctc agcagatttc
    11461 aactgagtac gaaattcagg tggaatcgca ggagctttct tttttggagc tgcagcttct
    11521 tttgaaaccg gctgttcgac tgcaatctct tgtggaactt ctggctct
//
LOCUS       DAOQNJ010000013        11558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_2533_length_11558_cov_7.228375, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000013 DAOQNJ010000000
VERSION     DAOQNJ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11558
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2533_length_11558_cov_7.228375"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLG52_00835"
     CDS             <1..798
                     /locus_tag="PLG52_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40009.1"
                     /translation="VTNEIQKLSTIKPDVAASLRQLVEEGSRTNPAILIPMDASLKTP
                     RDRVYPMNVEVPLADLNLLAMHAQTLNILEAYESASGGDRAVIFASFNKFQKAYLSTI
                     AGFGAYLTRAKEMAAQGENTAAGAIKLLAHLPLPIQRLLDKIPERFEVLNNLIKGREV
                     FSNVGAVVPSSTLTRFMTAKDDNAQKQLAWGIITDAKSVLRIHLRDFRPHVQALSSIG
                     RKDLASLITQDYLNAYAEGFNQYIRDLSRITTASRETMTKPQAKGNS"
     gene            801..2486
                     /locus_tag="PLG52_00840"
     CDS             801..2486
                     /locus_tag="PLG52_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB40010.1"
                     /translation="MKDPMIGMQLANFKVESLLGQGGMAMVYFGQDIKLHRPVAIKVL
                     DKRYKNHPAYAGRFVNEARMMAKWHHENIIQIYYADDTQGFPYYVMEYVDGQDLGSVM
                     DAYQADGNFMPHDEVLRIGKAIASALDYAHRHGVVHRDVKPSNILLSKDGRVLLGDFG
                     MALEVRDGSMGNIFGTPHYISPEQAKRSADAVPQSDIYSLGVIMYEVLAGAAPFNDSS
                     PANIALQHISQKPPAPSTINPSLSRAVESVLLKVLEKNVKDRYQTAMKFMDALEDALS
                     ASRVSEKASLPPLPVGVPTIHRNEYQPEQHSRKKVIENQAPVAKMPATVRANAPMPKR
                     KTWLWVVLVLLLLGFGGFYFLQNGFNLPALPSQSTATTESAAPLSTPQPVLPTDTPVP
                     SPTFTATETIVPPTATIEPSPTIVLTPTVKYPEGANYTLFYNETSLFIINRSYERRSI
                     SGFKFQNLNADGTLGEEVFDGYRWETRNVDYLPRNFCANISIFGDEDPPYLNPVDCQQ
                     YGSIQTVQTRFDRPEKILFWVPKGGTTQFVVFWLNEEVARCEITAGICEVYIP"
     gene            complement(2490..2861)
                     /locus_tag="PLG52_00845"
     CDS             complement(2490..2861)
                     /locus_tag="PLG52_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB40011.1"
                     /translation="MTHVITSLCLRDNGCSSVCPVESINPGNPAQGWYTYYIDPSSCI
                     DCGACVPECPFHAIYPADEIPQAYKAKGGELINMAGLNGHYEGMSHTGEVIRLDSTRA
                     LTAGEVMDLTPAIVLNEKFYK"
     gene            3192..5030
                     /locus_tag="PLG52_00850"
     CDS             3192..5030
                     /locus_tag="PLG52_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40012.1"
                     /translation="MNTHPTTSQYFDFRDALHENRLLGLWRMMTDFRLSYIAATISLA
                     FSALAKTSVYLLLRFFADDVLVQGKMIGSTMTQTFLWIGLGFIGLAFIEGGGAFLSGR
                     LAAYTAEGITRRLRDFLFDHIQRLSFSYHSATPTGDLIERVTSDVDALRRFFSEQAIG
                     VGRIVLLFAINWAAILYINVKLGLVSVVTIPLILWVSLWFFKKVTKAYEDYQAQEAVL
                     STTLQENLTGVRVVKAFARQDYEMEKFEKDNWLKYIKGKFLLLMHSLFWPLSDIVLGG
                     QMLFGFIFAGFMAINKEITVGDYVAYVGLLVWLIFPIRNLGRVIVQTSTGMVSYQRLM
                     DITKQAREDLNGGKVQPTGPVRGEIEFKDVSFMYSDGTHDVIQNVSFHIKPGESIALL
                     GSTGSGKTSMVNLLPRFHDYTGGQILLDGVDLKDYPRKYLREQIGIVQQEPFLFSRSI
                     RENILYGVGRSVTQAEIEDAAKAAAVHDVIIGFPDGYNTIVGEKGVTLSGGQKQRVTI
                     ARTILKNPKILILDDSTSAVDTETEASIRQALNDLMEHRTTFIIAHRIQSVMKADQIL
                     VLDKGQVVQHGTHQELVGQFGGMYRKVFDIQTRIDEELENEIARAN"
     gene            5073..6962
                     /locus_tag="PLG52_00855"
     CDS             5073..6962
                     /locus_tag="PLG52_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40013.1"
                     /translation="MSEELLELEEEEHTSQMTFPVFKRIMGIIKPHWRWVLGFLITIG
                     LVSGLDAYFTYLNKEIVDNGINLGDKAFLFKVAWIYGSLLLLQSIFVFTFIYLAGVLG
                     ERVQYDLRKLLFNHLQDLSLSYYAQNAVGRLIARVTSDTGRVSELVTWGIVDSVWAFM
                     NIVTSLTFMAIINWKLALIVSIIIPIMIWVANKFQSYILLEFRVTRRTNSKITGAYNE
                     NIQGVRVVKALGREDANLVEFQNLTSTMYRSSYRAAWLSALFLPTVQIIAAFALGAIV
                     WYGGMEISTGFITIGGIQAFVSYLTFMMWPVQDLARVYAETQHSIASAERIFKLVDTK
                     PEVHNRADAIEAKTLLGEIEFDHVDFFYEDRKPVLTDFTLKVQPGEMIALVGPTGGGK
                     STIVNLLCRFYEPQNGMIRINGHDYTQYTLESIHNKIGIVLQTPHLFSGTVRENIRYG
                     KLHASDAEVEEAAKIAGAHDFIMTLEKGYEQNVGEGGNLLSVGQKQLISLARAVLAKP
                     ELFIMDEATSSVDTLTEALIQKGMEALMKGRTSFVIAHRLSTIRRANRILVIEDGRIA
                     EHGTHAELLRARGHYYRLYTQQFRHELEVQYGVIEGETSNEREGVSADSIVAKREKSE
                     TVVAD"
     gene            complement(7003..9312)
                     /locus_tag="PLG52_00860"
     CDS             complement(7003..9312)
                     /locus_tag="PLG52_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB40014.1"
                     /translation="MEQNIKILFVDDDPEMRTLVSFILKRAGYEVELVESAAEAFQYL
                     THSTPNLILSDVMMADSDGFTLLDTLRKQEATRHIPVILLTALAAPSDLVKGLSLGAD
                     DYIQKPFQSAELLARIQSKINRPPMPSELVPMDIRSGLVKPEQFEEEFKKEFFRSLRN
                     GSEGFIAFLSLSELAITRERVGIDIAPEIWKQAARSIAADLGPTDVIGLANGNLLGII
                     LPDMLEATAHKVLVRLTRKIMNYTFVVGNERFRLTPSIGYANFLSAKNAADARNRALT
                     ALDNAIHHLDMEPRLYEPSMGSIEKKEKNSFFQSVAQVLEKLILPFQIGLTFLIGIIL
                     PYFIYRWMDFIGYDITPVAYMIVTISLVLTALLIWVEGFYALRKNPIPETPGAPYPPA
                     TAVIAAYLPNEGATIVDTIEAFLRLDYPAPLQIILAYNSPYEMPIERDLKEIAARDPR
                     FVPFRVSNSTSKAQNINTVIAKVTGEFIGIFDADHHPDADSFTRAWRWLSNGYSIVQG
                     HCAVRNGDFSWVSRLVAVEFEAIYAISHPGRARLHQFGIFGGSNGFWKVDLLRQTRMH
                     GFMLTEDIDSSIRVTMDGVKIASDPDLISRELAPTNLKTLWNQRMRWAQGWHQVSLKH
                     LLQALASKKLTIRQKFGAFWLLGWREAYPWLSMQMFPIILFWIQKYHGVENINWLIPI
                     FVMATLFTLSVGPGQVFFSYRLSLPEIKQHKGWFVFYFIVTSLVYTEFKNTIGRVAQI
                     KELFKERQWKVTPRSAPQPPHGQSRFVSR"
     gene            complement(9476..11437)
                     /locus_tag="PLG52_00865"
     CDS             complement(9476..11437)
                     /locus_tag="PLG52_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNB40015.1"
                     /translation="MINSNESVKSPIIERDTNLTPPRTFRSYFIGRPLSTADAPHQTI
                     GKAVGLAVFASDALSSTAYATQEILGVIAAAGTVAYGYLFPISLAIITLLAIVTISYE
                     QTIHAYPGGGGAYIVARDNLGELPAQTAGAALLTDYILTVSVSVSSGVAQIISAYPNL
                     FQYRVWIAVAAVMLIMLINLRGVKESGTAFAIPTYFFVVVMVLTVGYGMYRFFTGSLG
                     AVIDPPHFEAHNVISVITPFILLHAFANGTTALTGVEAISNGITAFKEPRSKNAGITL
                     IWMSLILGSLFLGLSFLTGKVQAVFSEEETVISQLARTIFEGRGALYYMLISGTTVIL
                     IMAANTAFADFPRLGALHAGDGFLPRQLTYRGSRLVYSRGIITLAVIASILIVIFQAS
                     VTRLIPLYAIGVFLSFTLSQAGMARRWWKIGHLKPGEEIVEPGSVLKFEKGWQYRMAI
                     NGFGSLCTAVVMVVFAVTKFREGAWVVLILTPILVTIFFSIHHHYKNVANNLSLDNFG
                     AVPPHQTRHRIIIPISGVHQGSLAALRYARMLSDDVTAVHVAIEPAEADKVKKKWELW
                     GEGTRMVMLNSPYRLFLEPMLEYITEISERRQPGETLTIVVPEFISNNMLGAALHTNT
                     ADLLRSQLKRQEGVVIINVPYQLREHVKN"
BASE COUNT         3032 a         2760 c         2818 g         2948 t
ORIGIN      
        1 gtcacgaatg aaattcaaaa gctttccacc atcaagccag atgttgccgc gtccttacgt
       61 caactggtgg aggaaggcag ccgcaccaac cctgctatcc tcatccctat ggatgccagc
      121 ctgaaaaccc cgcgtgatcg ggtgtatcct atgaatgtag aagttccgct tgcggattta
      181 aatctgctcg cgatgcatgc tcaaacgttg aacatactgg aagcgtatga atccgcttcg
      241 ggcggagacc gagccgtgat cttcgccagt ttcaataaat tccagaaggc atatctctcc
      301 acgatcgctg ggtttggcgc ttacttgacc cgcgccaaag aaatggcagc acaaggcgaa
      361 aacacagcgg ctggggcgat caaattgtta gcacatttgc ctctgcctat tcaaaggcta
      421 ttagacaaga ttccggaacg ctttgaagtg ctaaataacc tcatcaaagg gcgcgaagta
      481 ttttcgaatg taggcgccgt agtcccctca agtacgctta ctcgctttat gacagcaaaa
      541 gatgataacg cccaaaaaca gctcgcttgg ggtatcatca ccgatgcgaa atcggttttg
      601 cgaattcatc tgcgcgattt tcgtccgcat gttcaggctt tgtcatccat tggacgcaaa
      661 gaccttgcta gcctcattac gcaagattat ttgaatgcct atgctgaagg attcaatcaa
      721 tacatccgcg atctttcacg tattacaact gccagtcgag aaactatgac taagcctcaa
      781 gcaaaaggaa attcctagcc atgaaagatc cgatgatcgg aatgcagctc gccaacttca
      841 aggttgagag cctattaggt cagggcggta tggcgatggt ctattttggg caggatatta
      901 aactgcatcg accagttgct attaaggttc ttgataagcg ctacaaaaac caccctgcct
      961 atgcagggcg tttcgtcaac gaagcacgga tgatggcaaa gtggcaccac gagaatatca
     1021 ttcagattta ttacgccgac gatacacaag gctttccata ttacgttatg gaatatgtag
     1081 atggtcaaga ccttggctct gtcatggatg catatcaagc agacggaaat ttcatgccgc
     1141 atgatgaagt gctgcggatt gggaaagcga ttgccagtgc attagattat gcccatcggc
     1201 atggggtcgt gcatcgcgat gtaaagcctt caaatattct cctctccaaa gatgggcgtg
     1261 tattgcttgg tgattttggt atggcgcttg aagtgcgtga tgggtccatg ggaaatattt
     1321 tcgggacgcc tcattacatc tcgcccgaac aagccaaacg ttcggcagat gcggtccctc
     1381 aatctgatat ttattccttg ggcgtcatca tgtacgaagt ccttgcagga gcagcaccat
     1441 tcaatgactc ttccccagca aatattgctt tgcaacatat ttctcaaaag ccacctgctc
     1501 ctagcacgat aaacccgagc ctgtcgcgtg ctgttgagtc tgttttgttg aaagttcttg
     1561 agaagaacgt caaagatcgt tatcagaccg ccatgaagtt tatggacgca ttggaagatg
     1621 cgctgtcggc atcacgtgtc tcagaaaaag cttctttgcc tccgctccct gtaggtgttc
     1681 ctacaattca tcgtaacgaa tatcaacctg aacagcactc tcgaaagaaa gtaatagaaa
     1741 atcaagcacc tgttgcaaag atgcccgcta ctgttcgcgc aaatgccccc atgcctaagc
     1801 gcaaaacttg gctttgggtc gtcttggttt tactacttct tggattcggc ggtttctact
     1861 tccttcaaaa cggattcaac ctgccagcgt tgccttctca atcgacagct acaacagaat
     1921 ccgccgcgcc tctttctacg cctcaacctg ttctgccaac tgatacaccc gtcccctccc
     1981 ctacctttac cgcaacagaa acgattgttc ctcccaccgc tacgatcgaa ccatcgccaa
     2041 caattgttct aacgcctaca gtgaagtacc cagaaggcgc aaactacacc ttgttttata
     2101 acgagaccag tttgtttatt atcaaccgtt cgtatgaacg gcgcagtatt tctgggttca
     2161 agttccaaaa cctcaatgcc gatggaactt taggcgaaga agtttttgat ggctatcgtt
     2221 gggagacgcg taatgtagat tatctaccac gtaacttctg cgcaaatatc agcatttttg
     2281 gcgatgaaga tccaccttat ctcaaccctg tagattgcca gcaatacggt tctattcaaa
     2341 ccgtacaaac ccgcttcgac cgcccagaaa aaatcctctt ttgggttcct aaaggcggca
     2401 caacgcaatt tgtggtcttt tggcttaatg aagaagttgc ccgctgtgaa atcacagcag
     2461 gcatttgtga agtctatatt ccctgatctt tacttataaa atttctcgtt caatacgatt
     2521 gcaggtgtca agtccatgac ttcacctgct gttaaagcac gggtagagtc taaacgaatg
     2581 acttcgcctg tatgactcat cccctcgtaa tgaccattga gtcctgccat attgataagc
     2641 tccccgcctt ttgctttata tgcctgagga atttcatccg ccgggtaaat tgcatggaac
     2701 gggcattctg gcacacaagc accacagtca atacaactgg acgggtcaat atagtaggtg
     2761 taccagcctt gagcgggatt ccctgggttg atggactcca ctgggcaaac agatgagcat
     2821 ccattgtcac gcaaacatag gcttgtaatt acatgggtca ttttaggctc cttttgatat
     2881 attcatccag tcgcttacta gttataaaag ttatcggaaa aattaatttc taatccgtaa
     2941 cttaaccttg acatttttca aacgagaggt gtaaactcat aacgtaattc aaattccaaa
     3001 agaaaggagc gtactcttga tgttcaacac aatcttcatc caaactctcg atgcagttgg
     3061 caaattgcct tcttggagtg cgcctgttga ccgtccctag gtctctttca gttccttgtc
     3121 gtttttgggc tgtggcgcac gcgccgcagc ctttttattt aatcgttttt cgttatgttc
     3181 ggagaaacac catgaatacc caccctacca cctcccaata ctttgacttc cgagacgcgc
     3241 tccatgaaaa ccgcctgctc ggtctgtggc gtatgatgac agattttcgt ctgtcctata
     3301 ttgctgcaac gatctcacta gccttctctg ccctggcaaa gacatctgtg tacctcctcc
     3361 tgcgtttctt tgcagatgat gttcttgtac agggtaagat gatcggctct accatgaccc
     3421 aaacctttct gtggatcggg ctgggattca ttgggctcgc cttcatcgaa ggcggcggcg
     3481 cgtttctttc cgggcgttta gccgcttaca cagccgaagg catcacacgc cgtttacgag
     3541 actttctctt cgaccatatt caacgcttga gcttctcgta ccacagcgcc acacccaccg
     3601 gagacttgat cgaacgcgtc acttcagatg tggatgcctt acggcgcttc ttttcggagc
     3661 aagccatcgg cgtcggcaga atcgtgctgc tatttgcgat caattgggct gcgatcttgt
     3721 acattaacgt gaagttgggg ctggtttctg tagtgaccat tccgcttatt ttgtgggttt
     3781 ccttgtggtt cttcaaaaag gtaacaaaag cctatgaaga ttatcaggcg caagaggcgg
     3841 tcctctctac tacgttgcaa gagaacctga ccggggttcg tgtggtgaaa gcctttgccc
     3901 ggcaggacta cgaaatggag aagtttgaaa aggataactg gctgaaatac attaaaggca
     3961 agttcctttt gctgatgcac tcccttttct ggcctctttc ggacattgtc cttggtggac
     4021 agatgctctt tgggtttatt tttgctggct ttatggcgat caacaaagag atcaccgttg
     4081 gcgattatgt agcatacgta ggtttgctgg tttggttgat cttccccatc cgcaaccttg
     4141 ggcgtgtgat cgtacagacc tcaaccggta tggtttcata ccagcgtctg atggacatta
     4201 ccaaacaggc acgggaagac ctcaacggcg gcaaggtcca gccgacgggt ccggtaagag
     4261 gcgagatcga attcaaagat gtatcgttta tgtattctga tggaacgcat gatgtcatcc
     4321 agaatgtttc attccatatc aagcctggcg aatcgatcgc tttgcttgga tcaacaggtt
     4381 caggtaagac atcgatggtg aatttactgc cacgctttca tgattacacc ggcggacaaa
     4441 ttctgctcga tggtgtggac ctgaaagatt atccacgtaa atatttgcgg gagcagatcg
     4501 gtattgtgca acaagagccc ttcttgttca gtcgttcgat ccgagagaac attttgtacg
     4561 gtgtgggtcg cagtgtgaca caggcagaga tcgaagatgc tgcaaaggct gctgctgtac
     4621 atgacgttat tattggattc cccgacggct acaacaccat cgtcggtgaa aaaggcgtaa
     4681 ctctatctgg cggtcagaaa caacgtgtga ccattgcacg gacgatcttg aaaaatccga
     4741 agattcttat ccttgacgac tcaacctcgg cagtggacac ggaaacggaa gcgtccattc
     4801 gccaggcatt gaacgaccta atggagcacc gcacgacctt catcattgcg caccgcatcc
     4861 aatccgtgat gaaagcagac cagatcctcg tgctagataa aggtcaggtt gtgcagcacg
     4921 gaacgcatca ggaactcgtc ggtcagtttg gcggtatgta ccgcaaagtc ttcgatattc
     4981 aaacaaggat cgacgaagaa ttagagaatg aaatcgcacg agcgaattga ttgtgtatgg
     5041 gcggggtgac ccgcccatac ggagaataaa caatgtcaga agaattattg gaacttgaag
     5101 aagaagaaca cacatcgcaa atgaccttcc cggtcttcaa gcgcattatg ggaattatca
     5161 aaccgcattg gagatgggtg ctgggttttc ttatcacaat tggtctggtt tctggtttag
     5221 atgcctactt tacctatttg aacaaggaaa ttgtagacaa tggcatcaat cttggagata
     5281 aggcattcct cttcaaggta gcctggatct acggttcatt gctgttgctg caatccatct
     5341 ttgtgttcac gtttatttat cttgctggtg tgttgggtga gcgtgtacag tacgatttgc
     5401 gaaaactgct gttcaatcac ttgcaggact tgtcgctttc gtattatgcc caaaacgcgg
     5461 ttggacgttt aatcgcacgt gtcacctccg atactgggcg cgtctcagaa ctggtgacct
     5521 ggggtattgt ggatagcgtt tgggcgttca tgaatatcgt cacttcattg acattcatgg
     5581 cgatcatcaa ttggaaactg gcgctgattg tttcgatcat tatcccgatc atgatctggg
     5641 tggcgaataa attccaatcg tatattctgt tggagtttcg cgttacccgc cgcacaaact
     5701 ccaagataac tggtgcgtac aacgagaata ttcaaggtgt gcgcgtggtc aaggcgctcg
     5761 ggcgtgagga tgcgaatctg gtcgaattcc agaacttgac ctccactatg taccggtcat
     5821 cctaccgcgc agcttggctc tcggcgctct tcctccccac cgtgcagatc attgcggcat
     5881 tcgccttggg tgccatcgtt tggtatggcg gcatggaaat ctcaaccggc ttcatcacca
     5941 tcggcgggat tcaagctttc gtctcgtacc tgacctttat gatgtggccc gtgcaggacc
     6001 tggcacgcgt ctacgctgag acccaacaca gcattgcttc ggcggagaga attttcaaat
     6061 tggtagacac caagcctgag gtccataacc gagccgatgc aattgaggcg aagaccctgc
     6121 tgggcgagat cgagtttgat cacgtggact tcttctacga agaccgcaaa cctgtattga
     6181 ccgatttcac gcttaaagtt cagccgggag agatgatcgc gctggttggt ccgaccggtg
     6241 gtggaaagtc tactattgtt aatttacttt gccgctttta cgagccgcag aatggtatga
     6301 tccgaatcaa tgggcatgac tatacccaat acacgttgga gtcgatccat aacaagatcg
     6361 gtatcgtgtt gcaaacgccg catctgttct cagggacagt gcgtgagaac atccgctacg
     6421 gcaagctaca cgctagcgat gccgaagtgg aagaagctgc caaaatcgct ggtgcgcatg
     6481 atttcatcat gacattggaa aaaggctatg aacagaacgt aggagaaggc ggtaacttgc
     6541 tctctgttgg gcaaaaacaa ttgatctcgc tggcgcgtgc cgtgcttgcc aagccagaac
     6601 ttttcattat ggatgaagcg acttcttcgg tagatacctt aaccgaggct ctcatccaaa
     6661 aaggcatgga agctctgatg aagggacgta catcctttgt gattgcacat cgtctcagca
     6721 ccatccgccg tgcgaaccgc atccttgtga tcgaagatgg tcgtattgcg gaacacggca
     6781 cccacgccga actcttacgc gcccgaggac attactaccg cctatatacc cagcaattcc
     6841 gccatgagtt ggaagtgcag tatggggtga ttgagggaga aacaagtaat gagcgtgaag
     6901 gggtgagcgc ggattcgatt gtagcgaaac gcgagaagag tgaaactgtg gtggcggatt
     6961 aataagtaaa agatgtccct gagagttctc agggacatct ttttatctac tcacgaatcg
     7021 actttgacca tgcggcggct gaggagccga gcgcggggtg accttccact ggcgctcttt
     7081 gaataactcc ttgatctgtg ctacccgacc aatggtattt ttgaattccg tatataccaa
     7141 tgaagtaacg ataaagtaaa agacgaacca acctttatgt tgcttgattt caggcaggct
     7201 gagccgatac gagaagaaaa cctgacccgg tcccacgctc aatgtaaata gagtcgccat
     7261 cacgaagatg ggaatcaacc agttaatgtt ttccacgcca tggtatttct ggatccaaaa
     7321 caggatgatc gggaacatct gcattgaaag ccaggggtag gcttcacgcc aacccaacaa
     7381 ccagaaggcg ccgaactttt gtcgtatggt tagcttttta gatgccagcg cctgcaatag
     7441 atgtttcaag gaaacttggt gccagccttg agcccaacgc atacgttgat tccaaagagt
     7501 tttcaggttg gtgggtgcca gttcacgcga aatcaagtct gggtcggagg cgatcttcac
     7561 tccatccatt gtgacgcgga ttgaagagtc gatgtcttca gttagcataa aaccatgcat
     7621 gcgagtctgg cgcagcaggt caaccttcca aaatccgttc gagccgccga agataccaaa
     7681 ttgatgcaaa cgggcacgtc ccgggtgact gatcgcatag atcgcctcaa actcgacggc
     7741 gacaaggcgc gacacccatg aaaaatcacc attgcggaca gcgcaatgcc cctgcacaat
     7801 gctgtatccg ttcgaaagcc agcgccatgc gcgggtaaaa ctatcagcat ctgggtggtg
     7861 atctgcgtca aagataccta taaactcgcc agtaaccttg gcaattacgg tattgatatt
     7921 ttgagccttg gatgtactat tgctgacccg gaacggtaca aaacgcggat cgcgtgcggc
     7981 aatttctttc aagtcccttt cgatgggcat ctcgtatgga ctgttataag caaggatgat
     8041 ctgcaaaggc gcaggatagt ccaacctcaa aaacgcttct attgtgtcga caatggttgc
     8101 gccctcgttg ggcaaatagg cagcaatgac agcggtcgct ggcgggtatg gcgccccagg
     8161 cgtttcaggg attggattct ttctcagcgc gtaaaatcct tctacccaga tcagaagcgc
     8221 tgtaagcacc aacgaaatgg tcacgatcat ataggctaca ggggtaatgt cgtaaccgat
     8281 aaagtccatc caacgataga tgaagtacgg gaggataatg ccgattagaa aggttagccc
     8341 aatttgaaag ggtaggatca gtttctccaa aacttgcgcc acagactgaa agaagctgtt
     8401 tttctctttt ttctcaatag atcccataga cggttcgtaa aggcgtggct ccatatccaa
     8461 gtggtggatg gcattgtcca aagcagttaa ggcgcggttc ctagcgtcgg ctgcattttt
     8521 tgcagaaaga aaatttgcat atccaatcga aggcgttaga cgaaaccgct cattcccaac
     8581 tacaaatgta tagttcatga tttttcgcgt gagcctgact aaaactttat gtgcagtggc
     8641 ttccagcata tccggcagga tgatccccaa gagattccca tttgccagac cgatcacatc
     8701 cgtaggaccc aggtctgcgg cgatggatcg agctgcctgc ttccatatct caggtgcgat
     8761 atctatgccg acgcgttcgc gagtaatggc gagttcggat aaagataaaa acgcaataaa
     8821 cccttcagag ccattgcgta gggatcggaa gaactccttt ttaaattctt cttcgaattg
     8881 ttctggtttc accaacccac tgcggatgtc catgggcacc aactcgctag gcatgggcgg
     8941 acgattgatc ttcgattgaa ttcttgccaa tagttcagcc gattggaatg gtttctgtat
     9001 gtaatcatct gcgcccaagg acaacccttt tactaggtca ctgggggcag cgagagcagt
     9061 aagtaatatc actggaatat gcctggttgc ctcttgtttt ctgagtgtat caagcagagt
     9121 aaaaccatcg gaatctgcca tcatgacatc tgacaaaata aggttggggg ttgaatgggt
     9181 tagatactga aatgcctcag ccgcactttc gaccaactcc acttcgtagc ctgctctttt
     9241 gagaatgaag cttacaaggg ttcgcatttc cggatcatcg tctacaaata ggatttttat
     9301 gttctgttcc attttttagc tccttgaaag ataatctttc aatttcgctt agaaaatacc
     9361 ccaaaactat gtattaattc cattagggaa aacctgtatg aaacaaaaaa gacaaaaccc
     9421 tctatccatt ttgtaaggaa gagggttttg tctttgcagt tttttgttta attatttaat
     9481 tcttcacatg ttcacggagc tgatacggaa cattgatgat gacgacccct tcctgacgtt
     9541 tcaactggct acgcaataga tctgccgtgt tggtgtgaag agccgcaccc aacatattgt
     9601 ttgaaatgaa ctcaggcacc acaatggtga gggtctcgcc gggttggcgg cgttcggaga
     9661 tttcggtgat gtattccagc atcggctcaa ggaagaggcg gtatggcgaa ttcagcatta
     9721 ccatgcgtgt cccctctccc catagctccc atttcttttt aactttatcc gcttcggcgg
     9781 gctcaatggc aacatggaca gcagtgacat catccgaaag catgcgcgcg tatcgcagtg
     9841 cagccaatga gccttgatga acgccgctga tcggtatgat gatgcgatga cgggtttgat
     9901 gcggcggaac agcaccgaaa ttatcaagag agaggttgtt cgcgacattc ttataatggt
     9961 ggtggatcga gaagaaaatc gtcacaagga tgggagtaag gatcaaaacg acccatgcac
    10021 cttcacggaa cttggtcacc gcaaagacga ccatgacaac agctgtacat aacgatccaa
    10081 agccattgat ggccatgcgg tattgccagc ctttttcaaa cttaagcaca gagcctggct
    10141 caacgatctc ttcacctggc ttaaggtgac cgatcttcca ccaacggcga gccatgcctg
    10201 cctgtgacaa agtaaatgac aggaacacac caatagcata tagaggaatt agccttgtta
    10261 cactggcttg gaagataacg ataaggatgg aggcgatcac agctagagtg atgatgccac
    10321 gtgagtagac gaggcggctg ccccgatatg tcaactggcg aggcagaaac ccatcgccag
    10381 cgtgaagcgc gccaagacgt ggaaagtctg cgaaggcggt attggctgcc atgatgagga
    10441 tgactgtcgt gccagagatc agcatgtaat ataaagcgcc tctgccctcg aagatggtac
    10501 gtgccaactg cgagataacg gtctcttctt cagaaaacac cgcttgtact tttcctgtta
    10561 agaaggacaa accaaggaag agcgaaccaa ggattagaga catccagatc agggtgatac
    10621 cggcattctt gctacgaggt tctttgaagg cagtaatacc gttcgaaatg gcttccacgc
    10681 cggtgagagc cgtagttccg ttagcgaagg cgtgtaacaa gatgaacgga gtaatgaccg
    10741 agataacatt atgcgcttca aaatgcggcg gatcgatcac ggctcccaga gagcccgtga
    10801 agaagcgata catcccgtac ccaacagtca ataccatgac cacaacgaag aaatatgtgg
    10861 gaatggcaaa tgcagtacct gactctttca cgccgcgcaa gttgataagc atgatgagca
    10921 taactgccgc aactgcaatc caaacacgat attgaaataa gttggggtat gccgaaatga
    10981 tctgtgcaac cccagaggag actgagacag agacggtcag aatgtaatca gtcaataaag
    11041 ccgcaccagc cgtttgagcg ggcaactcac cgaggttgtc gcgggcgacg atataagcac
    11101 cacctccacc cgggtaggca tggatagttt gttcgtacga aatggtgacg attgccaaaa
    11161 gggtgatgat ggcaagcgag atcgggaaga gataaccata agctactgtc cctgctgcag
    11221 cgataactcc taaaatttct tgtgtagcat aggcagtgga cgataatgca tcggatgcga
    11281 aaacagccaa acccacggct ttgccaatgg tctgatgcgg cgcgtcggct gtggatagcg
    11341 gtctgccaat aaaatacgac cgaaaggtgc gtgggggcgt gaggtttgtg tcccgctcaa
    11401 taatggggga ttttacactt tcattactat taatcataca gaaaaaattc tcctgggatg
    11461 tggaacgcaa aatatgacac gttccaaaca ggaagagtct attcagttgt ctgtcaaatt
    11521 ggcatcaaga aagtatcaag agaggtcaag aaaaggtc
//
LOCUS       DAOQNJ010000014        11230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_2676_length_11230_cov_8.096107, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000014 DAOQNJ010000000
VERSION     DAOQNJ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2676_length_11230_cov_8.096107"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLG52_00870"
     CDS             <1..950
                     /locus_tag="PLG52_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40016.1"
                     /translation="EAESAFGDGNVYLEKLIEGARHIEFQIIADEHGNVIHLGERECS
                     IQRRHQKLVEEAPSPFLDEELRKQMGDVAVKAAQAVDYVNAGTIEFLVDKDRNFYFLE
                     MNTRLQVEHPVTELVTGIDIVAEQLRIARGRQLSYTQEQVQFKGHAIECRVNAEDPFN
                     NFIPSTGRITHSIIPTGPGVRVDTGVYPGFEITPYYDPMIAKLIVWGETRAQAILRMR
                     RALEEYRIVGVRTNIPFHQTLMDSHRFMGGQFDTRFVEERFSMEAAATLDEAQAEIAA
                     ILATLVSHKETERSAQIVRGNERDTSNWKWVGRWERMHR"
     gene            968..1465
                     /locus_tag="PLG52_00875"
     CDS             968..1465
                     /locus_tag="PLG52_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNB40017.1"
                     /translation="MKYITTIEDKQFEIEVVDDHHVRVGGRLLEVNFESVSGQPVYSL
                     ILDGKSYESYVYPGDEDWQVLLRGRQFQAKVEDEREKRLKAAAGGGVAEGGEFHLKAP
                     MPGLVVAIPIAEGQEVKKGEVLVILESMKMQNELKSPRDGTVSHIKVKAGESVEQKQV
                     LLNLH"
     gene            1468..2082
                     /gene="cyaB"
                     /locus_tag="PLG52_00880"
     CDS             1468..2082
                     /gene="cyaB"
                     /locus_tag="PLG52_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNB40018.1"
                     /translation="MSGQETEVKFFVQDLKRVEMRLQELEAHLIQPRVHEVNLRFDNE
                     AGELRKNFKVLRLRQDTEAKFTFKGPSEERAGGVLSRKEIEFSVGSFEAAKEFLELLG
                     FVPVVFYEKYRTTYEFLGVHIMLDELPYGTFVEIEGEDIETLQKAANLLGLNWNAMVR
                     AGYHALFERITEKYNLEYGQLSFEALKSITATAEDFGITYSDIN"
     gene            2172..2582
                     /locus_tag="PLG52_00885"
     CDS             2172..2582
                     /locus_tag="PLG52_00885"
                     /inference="COORDINATES: protein motif:HMM:NF023629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3601 domain-containing protein"
                     /protein_id="HNB40019.1"
                     /translation="MDNPFPTSWNIIAVLMGLFFITFLWGFLRILRARDIKNSQLIQS
                     RPSAPPPLPTPETEGTVYGLYPGWEYIVVQSFTDYYGNIFEVNQKLKFKGRHFLPYQG
                     GHTIMFEECSLYLQEDQNKNILDNFSSYIVKIKQ"
     gene            2612..3382
                     /locus_tag="PLG52_00890"
     CDS             2612..3382
                     /locus_tag="PLG52_00890"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNB40020.1"
                     /translation="MQIKGLDTLARHVPELNSSSGRMKIGVTFFAIFVLTTFYFIITD
                     QIPTWTLDSQIVVTALGYLILSRVFRQKKAFIEKHKEQAFAVAFKQFTVPSLAIFSAL
                     IIHIAYMNGPKFTQPTLTTILTWLGWVCILIGTPLFIRALTILGADHATMLYVYFPGG
                     ELTHSSIYNIVRHPMYAGMLRICIGLACLNRGIFALTFVIILPILVFGWIRLIEEKEL
                     IERIPNYIEYRKQVPAFFPYPKQIFRFFKFVLTGQSYA"
     gene            3375..3866
                     /locus_tag="PLG52_00895"
     CDS             3375..3866
                     /locus_tag="PLG52_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNB40021.1"
                     /translation="MLEKRPNSLLKFFFKTPLFMHKLGLGGWERLIGAQWMFLITIGR
                     KTGKRRETLVDVMDYDSATDTYFIEAAYGTRADWYKNIQAHPVFEAQVGRRKFNASAK
                     ELNSQDTGETLVQFFRKKPAYTRSVMAMVGLKFKDENELRALGDKLTLLAVTPLSHDR
                     SAK"
     gene            complement(3832..4650)
                     /gene="murI"
                     /locus_tag="PLG52_00900"
     CDS             complement(3832..4650)
                     /gene="murI"
                     /locus_tag="PLG52_00900"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNB40022.1"
                     /translation="MNLNPIGVFDSGVGGLSVLREIRNLMPNEDILYFGDQGHVPYGP
                     RPMEQIQDFSEGITRFLLKERSKIIVVACNTASAAALKYLRQAFPNVQFVGMEPAVKP
                     AAEKTQTGKVGVLATPATFQGALYASVVERFANGVELFQNTCNGLVQQIEKGNLDGEE
                     TRKILEGALHPMLEKRIDTVVLGCTHYPFVIPLIEKIVGENVRVIDPAPAVAKQVKRL
                     LEASEALNQKEQLGSTRFMTSGDVEVVKSSLKILLQLEREVEAVTWLNDREIAA"
     gene            complement(4669..5379)
                     /locus_tag="PLG52_00905"
     CDS             complement(4669..5379)
                     /locus_tag="PLG52_00905"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNB40023.1"
                     /translation="MTKPLIGITTFNRNNDYGKPMSGVQHTYIRAVVQGGGIPILIPA
                     ILDEEDRNDLYARVQGVLFTGGGDVSIKYFNGVDHPKIAEVDESRDITEISLLKKTVE
                     DGKPFLAVCRGVQVMNVALGGTLYTHIPDQFPNALHHDNEEFTEIIHPVNINEDSRMA
                     EIFGETLLNVNSLHHQGLKDVASPLKVVGHAPDGMIEAVELPDHPYAMGVQWHPEWLT
                     DQPVMRRLFKSFVDASLQ"
     gene            complement(5490..5837)
                     /gene="rplS"
                     /locus_tag="PLG52_00910"
     CDS             complement(5490..5837)
                     /gene="rplS"
                     /locus_tag="PLG52_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNB40024.1"
                     /translation="MADLIKAVEAKDNEKIPQLAAGDTVSVHVKIKEGDRERIQEFKG
                     LVIRLRNSGNNASVTVRRMASNGVGVERTFLLRSPRVDKIVVERHGKVRRAQLYYMRE
                     RTGKSARIKEKFS"
     gene            complement(5923..7167)
                     /gene="fabF"
                     /locus_tag="PLG52_00915"
     CDS             complement(5923..7167)
                     /gene="fabF"
                     /locus_tag="PLG52_00915"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNB40025.1"
                     /translation="MQERIVITGMGTVNPLGLNAQESWTNAINGVSGVAPITQFDSHE
                     AKLQVHIAAEVKNFKAENFMDAREARRRDRFEQFAIAASKEALSSSGLQVTEKNAGRI
                     GIIISSAIGGLYSLQEATLTNYKEGPRKVSPFMIPMLMANGGAGMAAIEFGIKGPCFS
                     VASACASGADGIGTALMMLRTGMIDAALAGASEATITSTGVAAFDRVGAMSRRNDNYS
                     MTPQPFDKNRDGLVMGEGAAVLVLERESDAKARGANILAELAGYGATADAFHVTAPHE
                     HGAGGAAAIRMALDSAKANIDEVGYINAHGTGTSLNDQAETRAVKAAFGDLAYKTPIS
                     STKSMTGHMMGATGALEVIFCVNAIRDGILPPTIHYETPDPECDLDYIPNKAREAKIS
                     LAISNAFGFGGHNAVLAIRKYS"
     gene            complement(7305..7829)
                     /locus_tag="PLG52_00920"
     CDS             complement(7305..7829)
                     /locus_tag="PLG52_00920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40026.1"
                     /translation="MVTIRKAVLKDASAIATVHVAAWKEAYRGIVADEVLNNLSIQRR
                     TEQWTNSLSDETHPYHRAFVAEMNSQVVGFSNFGAPQLKDLGFDGELFAIYILKAAHE
                     IGIGRSLVQAVVNGLREIGSKSMMVWVLKDNPARGFYERLGGVYLYEKPIQIGADELM
                     EVAYGWRDLSIFPV"
     gene            complement(7860..8414)
                     /locus_tag="PLG52_00925"
     CDS             complement(7860..8414)
                     /locus_tag="PLG52_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40027.1"
                     /translation="MHTQRLILRNFELADLDAYVGYRNDPEVGKYQGWVIPYSKEKGE
                     EFIREVQDMHAPKQGEWMQIAIALKETNVLIGDIGCVLRQEDARQAIIGYRIASPHWH
                     KGYAFEAVQAWLDFLFEDLNLHRVIADCDTENTASYKLLEKLGFRREAHFIESYLENG
                     GYTNEYHYGILQREWRAKAAFRNC"
     gene            complement(8424..8891)
                     /locus_tag="PLG52_00930"
     CDS             complement(8424..8891)
                     /locus_tag="PLG52_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNB40028.1"
                     /translation="MQPSNHPTIYSITESEIDLNDLLAKITLTSTGAAAIFTGMVRGV
                     TTRGDAHETEYLEYESYVPMAEAKMQQVADEIREKWPVVEGIAIVQRIGKLYPKTPTV
                     LIACTAAHRDTGVFDAARYGIDRLKEIVPVWKKEVGPNGEEWVEGDYVPKPGE"
     gene            complement(8901..9149)
                     /gene="moaD"
                     /locus_tag="PLG52_00935"
     CDS             complement(8901..9149)
                     /gene="moaD"
                     /locus_tag="PLG52_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNB40029.1"
                     /translation="MNHIKILFFATIRDRAGTKSIELDIPADATILQLKEKLSADYPN
                     LKESLKSVLVTINREYVFDEAVVPTNAEIALFPPVSGG"
     gene            complement(9151..9519)
                     /locus_tag="PLG52_00940"
     CDS             complement(9151..9519)
                     /locus_tag="PLG52_00940"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB40030.1"
                     /translation="MNYTSINLKEKLSKFSEHWSPKIIAQLNDYHLKLAKVQGEFVWH
                     DHPDTDEVFIVVQGHLDILFRDGKVSLDEGEMFVVPKGVEHKPVAEKECHILLIEPAG
                     LVNTGSAKDSELTAPNDVWI"
     gene            complement(9530..10009)
                     /gene="moaC"
                     /locus_tag="PLG52_00945"
     CDS             complement(9530..10009)
                     /gene="moaC"
                     /locus_tag="PLG52_00945"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNB40031.1"
                     /translation="MSKLTHLDEHGRAKMVDVSHKPDTTRTAVARGEVHMTKATFDLI
                     RAGQIKKGDVLTVAQIAGITASKRTSDLIPLCHPLPLSKVDVDLELDESLPGIVITAT
                     AKVTGKTGVEMEALTAVSVAALTVYDMAKAAEKTMHIQNIRLLEKHGGMSGDVINEP"
     gene            10045..10818
                     /locus_tag="PLG52_00950"
     CDS             10045..10818
                     /locus_tag="PLG52_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNB40032.1"
                     /translation="MDGFLGNQLGTYKVQTPVYEGPLDLLLSLIERAELDITTVSLAS
                     VTDQYLEHLRNVDQQKPDEISSFLVIATKLVQIKSEALLPRPPEREPGEEDPGEALVE
                     QLKLYKRYKEIAVWMEERQTKNLRTFLRVAPPPKVEPKLDLSNLTLEKLLAAAESAFA
                     KGKEKKPLGTVITAPKVTIREKIDYITKTLKNIQRMSFSGLITDKATRVEIVVTFLAL
                     LELVKRYRVTAKQDVLFGDIEFERSEDWKDDEEIEIEFE"
     gene            complement(10867..>11230)
                     /locus_tag="PLG52_00955"
     CDS             complement(10867..>11230)
                     /locus_tag="PLG52_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB40033.1"
                     /translation="HEMPFTGEPVLMAFHGGDKGFVLEELSDDEIVAGAMQTLRTMFG
                     SDIPEPEGFLITRWGQDPFSFGAYSHIPPFASGDDYDALAVPVDDVLFFAGEATSREY
                     PSTVHGAYLSGVRAAEEM"
BASE COUNT         3064 a         2828 c         2440 g         2898 t
ORIGIN      
        1 gtgaggcaga atctgcgttt ggcgatggca atgtctacct cgagaaattg atcgagggcg
       61 cgcgtcacat cgaattccaa atcattgctg atgaacatgg caatgtcatc cacttgggcg
      121 aacgtgaatg ttccattcaa cgtcgccatc aaaaattggt agaagaagcg ccctcaccct
      181 ttctcgatga agaattgcgc aagcaaatgg gcgatgtagc tgtcaaagcc gcgcaagctg
      241 tggactatgt caacgcaggg acgattgaat tcctagttga taaagaccga aatttctact
      301 tccttgaaat gaacacccgt ttgcaagtgg aacatcctgt gacagagttg gtcaccggaa
      361 tcgacatcgt cgctgaacag ttgcgcatcg cccgtggacg tcaactcagc tatacacagg
      421 aacaagtaca gttcaaggga catgcgattg agtgccgtgt caatgcagaa gacccgttta
      481 acaatttcat cccatccacg ggtcgcatta cccacagcat catccctact ggacccggcg
      541 ttcgtgtgga cacaggtgtg taccccggtt ttgaaatcac accatattac gatccaatga
      601 ttgccaaact gattgtttgg ggcgagacac gcgctcaagc tattttgcgc atgcgccgtg
      661 ctctggaaga atatcgcatc gtgggtgttc gcactaatat ccccttccac caaaccttga
      721 tggactcgca tcgtttcatg ggtgggcaat tcgacacccg cttcgtggaa gaacgcttct
      781 ccatggaagc cgctgccacc ttggatgaag cacaggcaga gattgcagcc atcctcgcta
      841 cgctcgtatc tcacaaagag acggaacgct ctgctcagat cgtccgtggt aacgagcgcg
      901 acaccagcaa ctggaagtgg gtcggtcgct gggaacgcat gcaccgctaa ttagaagagg
      961 ctatggcatg aaatatataa ctacaataga agataagcaa tttgaaatag aagttgtcga
     1021 cgaccaccat gtacgtgtgg gcggacgcct gctcgaagtc aactttgaat cggttagcgg
     1081 tcagcctgtc tattctttaa ttctcgatgg caaatcctac gagtcgtatg tataccccgg
     1141 tgatgaagac tggcaggtct tactacgcgg acgacaattc caagccaaag ttgaggatga
     1201 aagagaaaag cgtctgaaag cggctgcagg cggcggcgtg gcagaaggag gcgagttcca
     1261 tctcaaagcg ccaatgccag ggctggttgt cgccattcct attgccgaag ggcaagaagt
     1321 caaaaaggga gaggtacttg ttatccttga gtcaatgaag atgcagaatg agctaaagtc
     1381 accgcgcgat ggcacggtga gccatatcaa ggtcaaggct ggcgagagcg ttgagcaaaa
     1441 gcaagtgttg ctgaatctac actaaccatg agcggacaag aaaccgaagt taagtttttt
     1501 gtgcaagacc taaaacgagt tgaaatgcgc cttcaagaac tggaggcgca tttaattcag
     1561 ccgcgtgtcc atgaagtcaa tttacgattc gataatgaag ccggggaatt aagaaaaaac
     1621 ttcaaggtac ttcgcctgcg acaggatacc gaagccaaat tcacgttcaa aggtccaagc
     1681 gaagaacgcg cgggcggcgt actcagccgg aaagagatcg aattctctgt tggcagtttt
     1741 gaagcagcca aagaatttct agaattactt ggtttcgtgc cagttgtctt ttacgaaaag
     1801 tatcgcacaa cctatgaatt tcttggcgtg cacattatgt tagatgaatt accttatggt
     1861 acttttgttg aaatcgaagg tgaagatatt gaaacacttc aaaaggcagc gaatcttctt
     1921 gggttgaact ggaacgctat ggtcagggcg gggtatcacg cactgtttga gcgcattaca
     1981 gaaaagtaca accttgaata tggtcagttg agttttgagg cgttaaagtc catcactgcc
     2041 acggctgaag atttcggaat cacctattct gatataaact agaatttatg aaccgttcta
     2101 cgcctaatcc gctcttcact gctctattgc tttttgtcag ttttgttttc tttgccatgg
     2161 tgatatggat catggataat ccattcccta cttcatggaa catcatcgcc gttctcatgg
     2221 ggctgttttt catcacgttt ttatggggat tcttgcgtat cttacgagca agagacataa
     2281 aaaattctca acttattcaa tccagaccgt ccgctccgcc acctttacca actccagaaa
     2341 cagagggaac tgtctatgga ctttacccag gctgggaata catagtagta caatctttta
     2401 cagactacta tggcaatatc tttgaggtga atcaaaagct caaattcaaa ggacgccatt
     2461 ttttaccgta tcaaggcggg catacgatta tgtttgaaga atgtagcctt tatttacaag
     2521 aggaccaaaa caagaatatt ctcgataatt tttcgagtta tatagtcaaa atcaaacaat
     2581 aagctaacct gttttaaata aagagattgc catgcaaatc aaaggtttag atactttagc
     2641 aaggcatgtt ccagaattaa attcatcatc agggcggatg aaaatcggcg taacattctt
     2701 cgccatattt gttttaacca ccttttattt catcatcacc gaccaaatcc ccacatggac
     2761 tcttgatagc caaattgtcg tcactgcatt gggttatctt attcttagtc gagtcttcag
     2821 gcaaaagaaa gcttttattg aaaagcacaa agagcaagcc tttgccgtgg cattcaaaca
     2881 atttacagtg ccaagtcttg ctattttttc tgcattaatt attcacatcg cttacatgaa
     2941 tggaccaaaa tttacccagc ctacacttac aacaatcctc acttggcttg gctgggtttg
     3001 tattcttatt ggaacgcccc tatttattcg tgcgcttacc atattgggtg cagatcatgc
     3061 cactatgcta tatgtgtatt ttccaggcgg cgagctgaca cactcaagta tctacaacat
     3121 tgttcgtcac cccatgtatg caggcatgct tcgcatctgc attgggttag cgtgcctcaa
     3181 ccgcggaatt tttgcgctga cctttgtaat tattctaccc atccttgttt ttggttggat
     3241 ccgtctcata gaagaaaagg aactaataga acgcattccc aactatattg aatatcgaaa
     3301 acaggtgccg gcattctttc cctacccgaa acaaatattc cgctttttca aatttgttct
     3361 aacaggtcaa tcgtatgctt gaaaaacgcc ccaactctct actgaagttt ttcttcaaaa
     3421 ctcctctctt tatgcacaaa ctcggtttag gtggttggga gcgcctcatt ggggcgcaat
     3481 ggatgtttct catcaccatc ggcagaaaaa ccggcaaacg ccgcgaaacg cttgtagatg
     3541 tgatggacta tgactctgcc acagacacct acttcattga agccgcctat ggcacccgtg
     3601 cagattggta taagaacatt caagcgcacc cagtttttga agcacaggtc ggtaggcgaa
     3661 aatttaacgc cagtgctaaa gagttaaact cacaagacac tggcgaaacg ttggttcagt
     3721 tttttagaaa aaagcctgct tatacccgct ccgtgatggc aatggtcggg ttgaaattca
     3781 aagatgagaa tgagcttcgc gcattaggcg acaagctaac tctcttagcg gtcacgccgc
     3841 tatctcacga tcgttcagcc aagtaaccgc ttcgacttcc cgctcaagct gcaacaaaat
     3901 tttcaaactg gatttgacca cctcgacgtc acccgatgtc ataaagcgtg tgctcccaag
     3961 ttgctccttt tggttcaaag cttcgctcgc ctcaagcaga cgtttgacct gttttgccac
     4021 tgccggagct gggtctatca cccgcacgtt ttcgcccact attttttcga tcaacggaat
     4081 cacaaaaggg taatgagtgc atcccaacac aacggtatcg atcctctttt caagcatggg
     4141 gtgaagagca ccttctaaga ttttgcgagt ttcttcacca tccaaattcc ccttctcgat
     4201 ctgttgcaca agtccgttgc aagtattttg aaataactcc acgccatttg cgaagcgctc
     4261 cacgacagaa gcataaagcg ctccctgaaa cgtagccggt gttgccagaa cccccacttt
     4321 ccccgtctgt gttttctctg ctgctggctt aaccgccggt tccatgccta cgaattgaac
     4381 gttaggaaaa gcctggcgca aatatttcaa tgccgcagcc gaagccgtat tacacgccac
     4441 cacaatgatc ttggaccttt ccttcagcaa aaagcgcgta atgccctcag aaaaatcctg
     4501 tatctgctcc atcgggcgcg gaccgtatgg gacgtgcccc tgatcgccaa agtatagaat
     4561 gtcttcattt ggcatcaggt ttctgatctc tcgcaagaca gaaagtccac ctacaccgga
     4621 atcaaacaca cctatagggt ttaaattcat atcgccaaaa cccggcattt attgaagaga
     4681 cgcatccaca aacgatttga acagtcggcg catcacaggc tgatcggtaa gccattcggg
     4741 gtgccattga acgcccatgg catatggatg gtctggcaat tctacagctt cgatcatgcc
     4801 gtctggagca tgaccaacta cttttaaggg tgaggcgacg tccttcaatc cctgatggtg
     4861 caagctattg acgttcaaca aagtttcccc aaaaatctct gccatacgag aatcctcgtt
     4921 aatgttaact gggtgaataa tctcagtaaa ttcttcgtta tcgtgatgca acgcattagg
     4981 gaattgatct gggatatgcg tatacagagt gccgcctaaa gcgacattca tcacttgaac
     5041 cccgcggcaa actgcgagaa aaggctttcc atcttccact gtttttttca gcaatgaaat
     5101 ctcagtgata tcgcgcgact catcgacctc tgcaattttc gggtggtcta cgccgttgaa
     5161 atatttaata gacacatctc cgccgcctgt aaataaaaca ccttgcaccc gagcatacaa
     5221 atcgtttcgg tcttcttcat ccaaaatcgc cgggatcaag atcggtatgc ccccaccttg
     5281 aaccaccgca cgaatatagg tgtgttgcac gccagacata ggcttgccgt aatcattatt
     5341 gcgattgaaa gttgtgatgc cgattaatgg tttggtcaca ggttcctcca aaacaagaaa
     5401 tttacgaata taagcatacc acagccaaac aagcggttat aaacaaaaaa atcccgatgg
     5461 ggttgccatc gggattcaat aaggaaaaac taggagaact tttccttgat gcgggcgctc
     5521 ttgccggtgc gttcgcgcat gtagtagagc tgggcacggc ggaccttacc gtggcgttcc
     5581 accacgattt tgtccacgcg cggggagcgg agcaggaagg ttcgttcgac gccaacgcca
     5641 ttcgacgcca tgcggcgcac agtcaccgaa gcgttgttgc cgctgtttcg caggcgaatt
     5701 accaagcctt tgaattcctg aatacgctca cgatcacctt ccttaatttt cacgtggacg
     5761 ctcacagtgt cgccagcagc aagctggggg atcttctcgt tatcttttgc ctctacggct
     5821 ttaatcaagt ctgccatttc gttcttcctt actttatgat taggtttcca atatacgcga
     5881 gggcggatta tagcacggat aatctcaaat cacaagcaaa aatcaggaat attttcgaat
     5941 tgccagcact gcgttgtgcc cgccaaaacc gaaagcattg ctgattgcca gcgaaatctt
     6001 cgcctcacga gccttgttcg gaatatagtc caagtcacat tcagggtcgg gggtttcgta
     6061 gtgaatggtt ggcggcaaaa tcccgtctcg aatggcattc acacagaaaa tcacttccaa
     6121 agcacccgta gcacccatca tatgccccgt catagacttg gttgaggaaa ttggcgtttt
     6181 atacgccaag tcaccaaatg ccgccttaac agcgcgggtt tcggcttggt cgttcaacga
     6241 agttccggtg ccatgagcgt taatgtaacc aacttcatca atgttagcct tggcagagtc
     6301 taatgccata cgaattgctg ccgcaccgcc cgccccatgt tcatgcggag ccgtcacatg
     6361 gaaagcatca gcagtggcac catacccggc taattcagcc agaatatttg caccacgtgc
     6421 tttcgcatcc gactcacgtt caagtacgag aacagccgcc ccttcgccca tcaccaaacc
     6481 gtcccgattc ttatcaaacg gttggggggt cattgaataa ttgtcatttc ggcgagacat
     6541 agcgcctacg cggtcaaatg cagccacacc cgtagatgtg atcgtcgctt cagaagctcc
     6601 tgccagcgca gcatcaatca tgcccgtgcg gagcatcatt agcgcagtac caattccgtc
     6661 tgcaccagaa gcacaagccg aagccacaga aaaacatggt cccttaattc caaattcaat
     6721 tgccgccatg cctgccccac cattcgccat caacatggga atcatgaacg ggcttacttt
     6781 acgcggacct tctttgtaat ttgtgagagt ggcttcctgc aatgaataca aaccaccgat
     6841 cgcagaagaa ataataatgc caatacgccc ggcatttttc tcggttacct gcaatccaga
     6901 actcgacaga gcttcttttg aagcggcaat ggcaaactgt tcaaagcggt cgcggcgtcg
     6961 agcttcgcgc gcatccataa aattttctgc tttgaagttt ttcacttctg ccgcaatatg
     7021 tacttgcaac ttcgcttcat gtgagtcgaa ctgtgtgatt ggcgccacac cagacacccc
     7081 attaatcgca ttcgtccatg attcctgcgc attcaatcca agtgggttta cagtccccat
     7141 gcctgtaatc acgatacgtt cctgcatctc gcctccgacg ataaaaaaat gaatataacg
     7201 atattaacaa cacaggattc tcagaatcgt gacgccctcc atcgataaaa ttaaggcaac
     7261 gcttcatccc ccgcccttgc ctgatagccc tctaaaacat gggtctacac ggggaatatg
     7321 ctcaaatctc gccacccata cgccacttcc atcagctcgt ctgcaccgat ctgaataggc
     7381 ttttcataga gatatacacc acccaaacgc tcgtaaaatc cacgcgctgg gttatctttc
     7441 aacacccaca ccatcatgga tttactacct atttcccgca acccattgac aacagcctgc
     7501 accaatgacc ttcctattcc tatttcgtga gccgctttca aaatataaat tgcaaacaac
     7561 tcgccatcaa agcccaagtc ttttaattga ggtgcgccaa aatttgaaaa acccacgacc
     7621 tgactattca tctccgccac aaatgcgcgg tgatatgggt gtgtttcatc agaaagagaa
     7681 ttggtccact gctccgtccg ccgctgaatg gacagattgt tcaacacctc atccgccaca
     7741 atgccacggt acgcctcctt ccacgccgcc acatggacag tagcaatcgc agaagcatct
     7801 ttcaaaacag ccttacgaat agtgaccatc tttgctccct tcttaaggct cagaacaaat
     7861 caacagttac gaaatgcagc cttggcacgc cattcacgtt gaaggatgcc gtaatgatat
     7921 tcattggtat agcccccatt ctctaaatag ctttcaataa aatgagcttc gcgccgaaaa
     7981 cctagctttt caagcaattt atatgaggct gtgttttctg tgtcacaatc tgctatgaca
     8041 cgatgtaaat taaggtcttc aaacaaaaaa tccaaccagg cttgaacagc ttcaaaagca
     8101 tatcctttat gccaatgagg ggaagcaata cgataaccaa taattgcctg ccgggcatct
     8161 tcttgcctca atacgcaacc gatatcgcct attaatacat tagtctcttt caaagcaatc
     8221 gcgatctgca tccactcgcc ttgcttagga gcatgcatat cttgcacttc tcgaataaac
     8281 tcctccccct tctcttttga ataaggaatg acccaacctt gatatttgcc tacttctggg
     8341 tcgtttcgat agcctacata ggcatcaagg tcggcaagct caaaattccg caggattaat
     8401 cgttgggtgt gcaaaaacaa aacttattct ccaggtttgg gaacgtaatc gccttcgacc
     8461 cattcctctc cattgggtcc gacttctttc ttccaaacag gcacaatttc cttgaggcgg
     8521 tcaataccat aacgggcagc atcaaaaacg cctgtatcac gatgggcagc agtacaagca
     8581 ataagaacag tcggcgtttt cgggtagagt ttgccaatgc gctgaacaat ggcaataccc
     8641 tccactacgg gccatttctc gcggatttca tctgctactt gctgcatctt ggcttctgcc
     8701 atagggacat aagactcata ttcgagatac tccgtctcgt gagcatcgcc acgggttgtg
     8761 acgccacgta ccatgcccgt gaaaatcgcc gctgctcctg tggatgtaag cgtgatcttg
     8821 gcaagtaaat cgttcaagtc aatttcagac tcggtaatgg aataaatggt tgggtggttt
     8881 gaaggttgca tagttgaata ttatcctcca gatacgggag ggaaaagggc gatttcagca
     8941 tttgtaggga cgaccgcttc atcaaaaaca tattcgcggt ttatagtgac caaaacagac
     9001 ttaagcgact ctttcaagtt agggtaatct gcgctcaact tctcctttaa ctgcaagatg
     9061 gttgcatctg caggaatatc caactcaata gattttgtcc ctgcacggtc acggatagtg
     9121 gcaaaaaata agatttttat gtgattcatt ttatatccat acatcatttg gagcggttaa
     9181 ctccgagtct tttgcgctac cagtatttac cagcccagct ggttcaatca ataaaatatg
     9241 acattctttt tcagcaacag gcttatgctc cacgcctttg ggcacaacaa acatctcacc
     9301 ctcatccagt gaaactttgc catcacgaaa cagaatatca agatgacctt gaaccacaat
     9361 aaagacttcg tctgtatcag gatggtcatg ccagacaaat tcaccttgaa ctttggctag
     9421 cttcagatga tagtcattaa gttgagcaat aatcttcggc gaccaatgtt cgctgaactt
     9481 cgagagtttc tcttttagat taatagaggt gtaattcatg gttcacccat cacggttcat
     9541 taataacatc gccgctcata ccgccatgtt tttccaacaa ccgaatattt tggatgtgca
     9601 tggttttctc ggcagctttt gccatatcgt agacggtcaa cgcagcgacg gaaacagccg
     9661 ttagggcttc catctcaacg ccggttttgc cggtcacttt ggcagtagca gtgatgacaa
     9721 taccaggaag ggattcatcc aactcaaggt cgacatccac tttggagagt ggaagaggat
     9781 gacataacgg tatcaggtct gaggtccgtt ttgaagcggt gatgccggca atttgcgcca
     9841 cggtcaacac atcgcctttt ttgatctgcc cggcgcggat taagtcaaat gtcgccttgg
     9901 tcatatgaac ctcaccgcgt gcaacggcag ttcgggtcgt atctggcttg tggcttacat
     9961 ccaccatttt ggcacgacca tgttcatcta aatgcgttag cttggacata gcgaagatta
    10021 tacactggta taattcgcgg gcttatggac ggatttctcg gcaatcaact tggcacgtac
    10081 aaggttcaga ccccagtgta tgagggtccg cttgatcttt tgttgagctt aatcgagcgc
    10141 gctgagttag acattacaac cgtatcgctc gcttcggtga cagaccaata tcttgaacac
    10201 cttcgtaatg tagaccaaca aaagccggat gagatttcgt ctttcctcgt gattgccacc
    10261 aaattggtgc aaatcaaatc ggaagcgttg ctgccacgcc cacctgagcg cgaacccggt
    10321 gaagaagacc ctggcgaagc acttgtggag cagttaaaac tttacaagcg atataaagaa
    10381 attgccgtgt ggatggaaga acgccagaca aaaaacttac ggacgttctt gcgtgtggct
    10441 ccgcctccca aagtggaacc gaagctggac ctttccaatt tgaccttaga gaagctgtta
    10501 gcggcagcgg aatcagcatt tgcaaagggc aaagagaaga aaccgctcgg aacagttatc
    10561 actgcgccca aagtaaccat tcgcgaaaag attgattaca tcaccaagac cttgaagaac
    10621 attcagcgca tgagctttag tggtctcatt acagacaaag ccactcgcgt tgaaattgta
    10681 gtaacctttc ttgcattgct agagttggtc aagcgttacc gcgtcacagc caagcaagat
    10741 gtgttgttcg gcgatattga attcgaacgc agcgaagatt ggaaagacga cgaagagatc
    10801 gaaattgagt ttgagtagta ttaatcaaaa gcggatgagg caaaacctca tccgttttat
    10861 tttaacttac atctcttccg cagctcgtac tcccgagaga tatgccccat gaaccgtact
    10921 gggatattct cgtgaagtcg cttcgcccgc gaagaacaac acatcatcca ctggtaccgc
    10981 aagtgcatca taatcatctc cactcgcaaa aggcggaata tgcgaatatg caccaaacga
    11041 aaacgggtct tgaccccagc gagtaatcaa aaaaccttcg ggttcaggga tatcactccc
    11101 aaacatcgtc cgcaaggttt gcatagcgcc tgcaacaatc tcatcatcgc tcaactcttc
    11161 aagcacaaaa cctttgtcgc cgccatgaaa tgccattaac acaggctcac cagtaaatgg
    11221 catttcatgg
//
LOCUS       DAOQNJ010000015        11129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_2720_length_11129_cov_9.387304, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000015 DAOQNJ010000000
VERSION     DAOQNJ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2720_length_11129_cov_9.387304"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..575)
                     /gene="rplI"
                     /locus_tag="PLG52_00960"
     CDS             complement(42..575)
                     /gene="rplI"
                     /locus_tag="PLG52_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNB40034.1"
                     /translation="MKVLLIKDVYKLGRAGDIKKVADGYGRNFLLPQGLAVLASEGAM
                     KQVQKIKAQAEIRRSTQNNELKGVADLIKDVTLIFPAKAGDTGKLYGSITTGDIATAL
                     SEKIRFEVKRQQVDTQPIRTLGEFTAHVRLTMDLVPDIKIIVHREGESPESAAGQHEE
                     PAKAEKKEEAAAETKAE"
     gene            697..1074
                     /locus_tag="PLG52_00965"
     CDS             697..1074
                     /locus_tag="PLG52_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40035.1"
                     /translation="MNQFPIKFRRIAVFAGIFILVLFVIEFNSRLEELNRLNEQRDEM
                     RAQATQAVQTQIALQTQVAYAASTDAVEEWARTDGHYLQEGDQPVIPVEQPGSDPVII
                     ETPIPVPTPMQNWEIWWTLFFDK"
     gene            1090..3621
                     /locus_tag="PLG52_00970"
     CDS             1090..3621
                     /locus_tag="PLG52_00970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB40036.1"
                     /translation="MEPALAVYLFCLAVLVAILLGIAIRAFSHWKTPGTRTLGALILT
                     MAVWAAFYILEVIHPSLEVKILARKMLYFGMTMAPPFWLAFALRYTGLGAWWSQQGRV
                     FLFAIPGGIAFLFGLTNEFHHLIWSDLSLSATKPVLLTIEYGIGFWIYAILAYLQIFA
                     GLAIYLVAHFKNKKAFRTKTGIVLAGLLLTAIVNVLFLLFGNSLNVDPTPLSFALSAP
                     LIAWGFFRFGVSSLFPLAASLVVENLQDAIIIVNREDEITDINQTAAELLNLKTVKEK
                     SNIFTLLPFAEQLKPIWDAQKSSIKLETNFRGASQWVEAQVIPIKDHEDSLLGRVIVL
                     HDITKEQALLRAERRRSQQLSLLEQTGRYIAGSFDEQEILQRTVDAITQQFGYPETAI
                     SLLKNNMFEIAAIFGTEDFGYRQGYRQEIGSGIIGYTASIKKTYVSRNVSQDPYYYST
                     STKSGSAICTPIFKQDALYGVLYVESFDLDAFDELDIITLETLASQMSASLQRAELYA
                     QTQNDLLTLRTIQNISKLITSSLDLETISQTVVRSLKESFDYTHVSIYLLEDEYLHLA
                     AEVGYPKELVIYKIHISQGVIGKAYRTRSIQFIEDTTKEKVFLKADNHITSEICIPLL
                     KEDIVLGMLNVESNVSNKLSQRDVDLLTTIAGPIAIAVDNARLHTEIKRMATTDAVTG
                     LSNRHVFEQALLAEFERAERSHSQMSLIIFDIDFFKQYNDIWGHPAGDARLKGVANII
                     KLNLRKYDIAARYGGDEFAIILANCDQQSAMTFAQRLQQGTIAGAPQQSENDAGIPGY
                     TLSIGIATYPQDATLPSELLVAADNAALRAKLHGRNQIKFANDYE"
     gene            3614..4930
                     /gene="lysA"
                     /locus_tag="PLG52_00975"
     CDS             3614..4930
                     /gene="lysA"
                     /locus_tag="PLG52_00975"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNB40037.1"
                     /translation="MNRLNLFPITTKIKNEKLNLAGHDLSQLAEQYGTPLYLYDRATL
                     DSATSEYKSAIASFYPAPASVTYAGKAFLCLAIAQWTQFHGLTVDCTGEGEIAFVVAA
                     GVSKENILVHGVNKSEDDLKSAIKYASTIVVDNLNELKRIQSLFTADNTTFPALWLRL
                     LPGVSVTTHHAHTQTGHHESKFGMTYEEILEAAKFCKENNLPLKGIHFHQGSNFRDPE
                     PLLPAIDLALDLAKEIGFTSDWHFCPGGGWGVAYHEDELPSPSIESYVRGISEAVVDG
                     CRMRGLTLPHLRLEPGRSLVAQAGVAMYRVGAIKKRGHKTWLLTDGGMADNPRFALYG
                     ARYSCLTVSSPSGERSERVSIAGPYCESGDIVIEDLPMPKVKEGDLIAIPVAGAYHLS
                     MSSNYNGARKPAVLWLEEDHVQLIRQRETVQDLFEADLKLAVLDKL"
     gene            complement(4901..9973)
                     /locus_tag="PLG52_00980"
     CDS             complement(4901..9973)
                     /locus_tag="PLG52_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB40038.1"
                     /translation="MPKIQNEKFVSSLDEWRADLLNLILGGIFVFWVLAFIGSIRNVI
                     DLYNKSQSLLNNPKLVAISVIVFYGIVTTLVGYVTFNRRLNVNLRAGILLFSLYALGT
                     SGMLLTSFSGDGRVLLFAFVIFSGIFFNLRYSLAAFFVTLLTLVVIGYVQISGLMVVP
                     PEWQVNAANPGAWFSGGLVLIVLSVAGIYSIAYLLQSFEASLKETQEALQREQRLGGV
                     LRTVSEINQIIVRAQDPEKMLHDACELLVAGQGYSFAWVGLLDEDMVTLRHIAHAGGD
                     VDESEFTTRLDLESGLTCAIQAIRTRSFFIVNPSDRNGDPCEKCPRIGKFPNRSAISL
                     PLQRDAKIFGVLVVDHTELAAAFDEQEISLLVELAGDLSYALENLEASKKLDTIARYQ
                     TLLNEVTQTALSAFDLDTLLRDFIEKLEKALDADGYYFAMWDEARGVPSKFIFSQKFH
                     DIFVTPPLLEPDDKFFTRAILEEKRILIIQNVMDTPYINPAFAEQFNVQSAMGIPLIA
                     DQMRIGALVLGYSDQHVFTSEEVKLADQSMNQFALAILKVKLNDEMRAKAKELEQLYT
                     AAQDMTASLMDPPALLKEMARHMAEALDVTSTYITSFDLQVMSMKVVAEYWGKESAPA
                     ELKSDLGRDYPSADYATVLKAMMEGRSLVLHAGQPNLTSEEYQQFVDYHIETMMFVPI
                     YSRGRLLGTVELWESRKRREFSQAEIRLANAMTSHAANIIENSTLFALTRKRESELSA
                     LLSVTRAISSSLQLPDVLREATTTLARMMRVDFCSISDYLPESNLLITRAIYSAEDDV
                     SEQGDIGKEFSLEHYPATLYVLQTGKPLVTRMDDAGADPAEVQQLKNDGMFSSLLIPL
                     RVRGYSLGLAELFSSDESRHFQQEEIQFASTLADQVAVAIENAYLYERLEQREAYFRA
                     LVENSTEGVAILDQQGIVRYLAPAEERLTGYSVDEIVGGSAFKYIHPDDLSKVLDMFQ
                     QGIENPGVVLSVQYRLKMKNGEWGHFEVSGTNMLGVPDIAGIVVNYRNITERVRTAKA
                     IEESEERYRTIFESAGIPIWEDDYSEFMASIEELKRKGITDFRKYFTEHPEFVERAAS
                     MIRITGVNAAAMSLMGATTKSQLIGALDSVLFSQPSNRFIDDVVALTDGRRHIEQESE
                     LFTLSGERRDVWVTINLPERAKGYERVLVSTLDITERKQIENALIESQGRLLGIVTTA
                     PNGIITVDANIRIILMNPAAEKIFGYLAEEVLGQSLNMLLPERFREHHAKYIQAYSAS
                     GVSHRKLGRYDSLYGIRKNGEEFPMEAFISRYEMEGEKFFTVILQDITVRKQDEDNLR
                     RRAEELQTLVIVSSALRSAVKVDEIIPLVARYSAEIVGGTYSTVYLLDQSSDALVSPG
                     WYSVRNGEDIKTTTAPLLRNASGNGITGYVASTGNIYITEDLRNDPKASILPDEIETL
                     RDAHSGISLPLLSQEMVIGVLHVRLTEKHVFNETEIRLLTAIAEMAGNALHRTKLYEQ
                     TVQQSEELAQAYDNTLSGWARALELRDELTEGHTRRVTELTLELARAMNISESELVQI
                     RRGAILHDIGKMGIPDAILNKPGPLTAHEIRIMQMHTQYAYEMISFIPFLKPAIDIPY
                     CHHEWWNGNGYPRGLKAEEIPLSARIFAVIDVWDALTSDRPYRAAWSTENALKYIQDG
                     SEKQFDPKVVDAFLKLIENRKL"
     gene            10216..11091
                     /gene="cyoE"
                     /locus_tag="PLG52_00985"
     CDS             10216..11091
                     /gene="cyoE"
                     /locus_tag="PLG52_00985"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNB40039.1"
                     /translation="MSARDSVPSSKFAMFWSLTKPSQSGLLLATGLAGYMSARCPVFN
                     LSTILQLSVSLFLAIAGSTLLNMWWDRDIDSKMGRTQKRVTSKGLVTDKEVLAVGILT
                     SVIGIGWALGMDWLYGVVVFAGWFFDVVVYSMWLKRRTCWSIVWGGISGAMPILAGRA
                     LGLGSIDWIGIMLAFGILLWIPTHTLTFSMKFQKDYSDAGVPTFPSTYGLDITRFTIA
                     FSSVLAAGAMVAAGVGIGMDWGFLRLLGVLSAGLLVLATAMTFKPTELVNFGLFKYAS
                     VYMLAAMILVVLEVI"
BASE COUNT         3026 a         2650 c         2380 g         3073 t
ORIGIN      
        1 aaacatcacc gacttgcgcc ggtgatgttt tgacattttt gttattccgc tttggtttct
       61 gctgcggctt cttctttttt ctcagctttg gcaggttctt cgtgttgacc agcggctgat
      121 tcggggcttt caccttcgcg gtggacgatg atcttgatat caggaaccaa gtccatggtg
      181 aggcgaacgt gggcagtgaa ttcgccgagc gtgcggatgg gttgggtatc tacctgttgg
      241 cgcttgactt caaagcggat cttttcgcta agcgcagtgg cgatatcacc ggtggtgatg
      301 gaaccgtaaa gctttccggt atcaccagcc ttggcgggga agataagagt tacatctttg
      361 atgaggtcag caacgccctt aagttcgttg ttctgggtgg agcggcgaat ctcagcttgc
      421 gccttgattt tctgaacctg cttcatcgct ccttcagaag cgagaacagc aagaccctga
      481 gggagaagga agttgcgtcc gtaaccatcg gcaactttct tgatgtcgcc ggcacgaccg
      541 agtttgtaaa cgtctttgat gagtaatact ttcatgattt aagccctttc tggcaaaatt
      601 tgaagtcagg tcgaagggta caacgacccg cggggcaaga ttttaccaca attgaatggt
      661 ttgccaatga cagagattaa ggttaagata atgggcatga accagttccc tatcaagttt
      721 aggcgaattg cagtctttgc aggcattttt attcttgtcc tttttgtgat tgaatttaac
      781 tcccgcctcg aagaactcaa tcgtttaaac gaacagcgtg atgagatgcg agcccaagct
      841 acgcaagctg tccaaaccca gattgcgtta caaacccagg ttgcttatgc tgcatcaaca
      901 gacgccgtcg aagagtgggc acgcaccgat ggacactacc tacaagaagg cgatcaaccc
      961 gttattccgg tagaacagcc tggtagtgac cccgtcatca ttgaaactcc cattcccgta
     1021 cctacaccaa tgcaaaactg ggaaatctgg tggacacttt tctttgataa gtaaccataa
     1081 ctttcctata tggaacctgc cctagcagta tatttatttt gtttagcagt tttagttgct
     1141 atattattgg ggatagcgat tcgcgccttt agccattgga aaacgcccgg aacccgaacc
     1201 cttggggcac tgatattgac aatggcagtt tgggcggcat tttatattct tgaagtcata
     1261 catccttctc ttgaagtaaa aattctagcg cggaaaatgc tttacttcgg tatgaccatg
     1321 gcaccgccat tttggttggc attcgcattg cgatacacag ggcttggagc gtggtggtca
     1381 caacaagggc gtgtcttttt attcgccatt ccgggtggaa ttgcattcct gtttggctta
     1441 actaacgaat ttcatcattt gatttggtca gacctttctt tatccgcaac caaaccggtt
     1501 ctgcttacta ttgagtatgg catagggttt tggatttatg caattcttgc ctaccttcag
     1561 attttcgcgg gtcttgcgat ctatcttgta gcacatttca aaaacaaaaa agcctttcgt
     1621 acaaaaacag gtatcgtttt agctggttta cttttaacag ccattgtcaa tgtccttttt
     1681 cttctttttg gaaacagctt aaacgtagac ccaactccgc tcagctttgc attatctgca
     1741 ccattgattg catgggggtt tttccgtttt ggagtttcaa gtctatttcc cctcgcggca
     1801 tcgttggtgg tagaaaacct tcaagatgca atcatcatcg taaaccgtga agatgagatt
     1861 actgacatca accaaacagc agctgaatta ctaaacttaa aaaccgtaaa agaaaaatca
     1921 aatattttca cgcttctgcc ttttgcagag caacttaaac cgatatggga tgcgcaaaaa
     1981 agctcgatta agttggaaac gaattttaga ggtgcatcac aatgggttga agcccaagtc
     2041 atccccatta aagaccatga agatagttta ctgggcaggg taattgttct acatgacatt
     2101 accaaggagc aagctctgtt gcgtgcagag agacgtcgct cccaacagtt atccctactg
     2161 gagcagaccg gcaggtatat tgctggtagc ttcgacgagc aagaaatcct tcaacgaact
     2221 gtggatgcca ttacacaaca atttggctac ccagaaactg ccatttcttt attgaaaaac
     2281 aatatgtttg aaatcgccgc aatctttggc acagaagatt tcggctaccg tcagggatac
     2341 agacaagaaa ttggctcagg catcattggg tataccgcca gcattaagaa aacttatgta
     2401 tctcggaacg tctcgcaaga cccctattat tattcaacaa gtacaaagtc tggatcggca
     2461 atctgtaccc cgatcttcaa acaagatgct ctctacggcg ttctatatgt agaaagtttt
     2521 gacctagatg ctttcgatga gctggatatt attacccttg aaacccttgc aagtcagatg
     2581 tccgcatcac tacaacgcgc cgaactgtat gcacaaaccc aaaatgacct acttactctg
     2641 cgaacgatcc aaaacatttc aaagctcatt acaagttcgc tcgaccttga gaccatcagt
     2701 cagaccgttg tgcgcagctt gaaagagtct tttgactata cacacgttag tatttatctc
     2761 cttgaagacg agtacttgca tctcgcggca gaagtgggct accccaaaga attggtgatc
     2821 tataaaattc acatcagtca gggagtgatt gggaaagcct atcgtacacg gtctatccaa
     2881 tttattgaag atacaactaa ggaaaaggtt ttcctcaagg cagataacca catcaccagt
     2941 gaaatttgta tccctctact aaaagaagat atcgtacttg ggatgcttaa tgttgaatcg
     3001 aatgtctcaa acaagttatc tcaacgggat gtagatttgt tgacaaccat tgctggtccc
     3061 attgcaatcg cggtagataa tgcccgttta catactgaaa ttaaaagaat ggcaacgacc
     3121 gatgccgtga ctgggctgtc aaatcgccat gtctttgaac aagccctgct cgcagaattt
     3181 gaaagagccg agcgaagcca ttcgcaaatg tcgcttatca tttttgatat cgactttttc
     3241 aaacaataca atgacatttg gggacacccc gctggagatg cccgcctgaa aggcgttgcc
     3301 aacatcatca aattaaatct tcgcaaatac gatattgccg cacgctatgg cggcgatgaa
     3361 tttgcgatca ttttggcaaa ttgcgaccaa caaagtgcca tgacatttgc tcaacgtttg
     3421 cagcaaggta cgatagcagg cgcacctcaa caatcagaaa acgatgcggg cattcccggg
     3481 tatacactca gcattggaat agccacctat ccgcaagatg caaccttacc ttcagaactt
     3541 ttggtcgccg cggataacgc cgccttgcgc gccaaactac atggacgaaa ccaaatcaaa
     3601 tttgcaaatg actatgaata gacttaacct cttccccatc acaacgaaaa tcaagaatga
     3661 aaaactgaat cttgccgggc acgatctttc tcagctcgca gaacaatatg ggaccccgct
     3721 ttatttgtat gaccgtgcga cattggattc ggcgacttcg gaatacaagt cggcgatcgc
     3781 ttctttctac cccgcgcctg cttctgtaac ctatgcaggg aaagcattcc tctgccttgc
     3841 cattgcacag tggactcaat tccatggctt aaccgtcgat tgcacaggag agggcgaaat
     3901 tgcctttgta gtagctgccg gggtttcaaa agagaacatt ctagttcatg gggttaacaa
     3961 gtctgaagac gacctaaaat ccgcaataaa gtatgcatcc acaatcgtcg ttgataatct
     4021 aaatgagctt aagcgcattc agagcttatt tactgctgac aatactacat tcccagcatt
     4081 gtggcttcgc ctattgcccg gagtttctgt caccacacat catgcccata ctcaaacagg
     4141 acatcacgaa agcaaattcg gcatgaccta tgaggagatc cttgaagctg cgaaattttg
     4201 taaagaaaac aacttgccac tgaagggtat ccattttcat caaggttcta acttccgtga
     4261 cccagaaccc ttacttcctg ccatagattt ggctcttgat cttgccaaag aaattggttt
     4321 cacaagtgat tggcattttt gccccggtgg cggttgggga gtggcatatc atgaagatga
     4381 attgccttcg ccatcgattg aaagttatgt ccgcgggatt tctgaagcgg tagtagatgg
     4441 ttgccgaatg cgtgggctta cactccccca tttacgtctt gaaccaggtc gtagcctagt
     4501 tgcgcaagct ggtgtggcaa tgtatcgagt gggtgctata aaaaagcgcg gtcacaaaac
     4561 ctggctactt acagatggag gcatggcaga taacccgcgc tttgcacttt acggggcaag
     4621 atattcttgt ttaacggtat caagcccgag cggagaaaga agcgagcgcg tctccattgc
     4681 gggaccgtat tgtgagtctg gggatattgt gattgaagat ttgccgatgc caaaagtcaa
     4741 agagggagat ttgattgcga tccctgtagc gggggcatac catttgagta tgtcatcgaa
     4801 ttataacggc gcgcgcaaac ctgctgtttt atggctggag gaagaccacg tgcaattgat
     4861 ccgacaacgt gaaacggttc aggacttgtt tgaagcggat ctaaagcttg cggttctcga
     4921 taagctttag gaaggcatct actactttag ggtcaaactg tttttcggaa ccgtcttgga
     4981 tatatttcaa cgcattttct gtagaccaag ccgcgcggta tggacggtct gatgttagtg
     5041 catcccaaac atcgataact gcaaagattc gggcagagag tggaatctct tccgctttca
     5101 acccgcgtgg atacccgttg ccattccacc attcatgatg acaataaggg atgtcgatag
     5161 ctggttttag gaacgggata aatgagatca tttcgtatgc atattgcgta tgcatttgca
     5221 tgattcgaat ttcatgcgct gtcaatggac ctggtttatt gagaattgca tctggaatac
     5281 ccatcttccc aatatcatgc aaaatagcac cacggcgaat ttgcaccagc tcgctttccg
     5341 aaatattcat agctcgtgcc agttccagcg ttagttcggt tacgcggcga gtatgcccct
     5401 ctgtcaactc atcgcgcaat tccaatgcac gtgcccagcc agaaagagtg ttgtcgtatg
     5461 cttgagccag ttcttcagat tgctgcacgg tctgttcgta aagttttgta cgatgaagag
     5521 cattgcccgc catttctgca atggctgtaa gtaagcgaat ttcagtctca ttgaaaacat
     5581 gcttctctgt tagacgcaca tgcagaaccc caataaccat ttcttgagaa agcagtggaa
     5641 gtgatatgcc actatgggca tcacgaagcg tctctatttc atctggcaag atagaagcct
     5701 ttgggtcatt acgcaaatct tcagtaatgt aaatattgcc cgtgctagct acatacccgg
     5761 ttataccgtt tccagaagca ttccgcaata gcggtgctgt ggtggtcttg atatcttccc
     5821 catttcgtac cgagtaccat cccggcgaga ctaaggcgtc ggaagattgg tctaaaagat
     5881 agacagtgct ataggtgcca ccaacgatct cagcagaata gcgtgccacc aatggaataa
     5941 tctcatccac ttttacggct gaacgaagag cagaagagac aatgaccaag gtttgtaatt
     6001 cttccgctcg gcggcgcaaa ttatcttcgt cttgtttgcg aacagtaata tcttgcaaga
     6061 ttacagtaaa gaacttctct ccctccattt cataacgtga aatgaaggct tccatgggaa
     6121 attcttcacc attcttgcga ataccatata acgaatcata tctaccgagt ttacgatggg
     6181 aaactccgct tgcactatag gcttgaatat actttgcatg atgttcccgg aaacgttctg
     6241 gaagcaacat atttaggctt tgccctaaaa cttcttctgc cagatagcca aatattttct
     6301 cagccgctgg gttcatcaag ataatgcgga tattggcatc gactgtaata atgccattgg
     6361 gcgctgtagt cacaataccc aaaagcctgc cctggctctc gattagagca ttctcaattt
     6421 gttttctctc tgtaatatct aatgtactaa caagaactcg ttcataccct tttgcacgtt
     6481 caggcaaatt aatggtcacc cacacatcac ggcgctcgcc agacaaagta aacaattcgc
     6541 tctcttgctc aatatgtcta cgtccatctg tcagtgccac aacatcgtca ataaatctgt
     6601 ttgatggctg agaaaataaa acactatcaa gtgcaccaat tagttgtgat tttgtggttg
     6661 cgcccattaa actcattgca gcagcattaa cccccgtaat gcggatcata cttgcagcac
     6721 gctcgacaaa ttccgggtgt tcagtaaaat atttacggaa atcggtgata cctttccgtt
     6781 ttaattcttc aatacttgcc ataaattcag aataatcatc ttcccaaatt ggaatcccag
     6841 cagactcaaa gatggtgcga tatctttcct cagactcttc gatggctttt gcagttcgaa
     6901 cccgttctgt aatatttcga taattaacta caatcccagc aatatctgga acacccaaca
     6961 tattggttcc acttacttca aaatgccccc actcaccatt tttcattttc aatcggtatt
     7021 gaacagaaag gacgacacca ggattttcaa taccctgttg aaacatatct agcactttgg
     7081 ataagtcatc gggatgaata tacttaaatg cgcttccgcc aacgatttca tcaacagaat
     7141 accccgtcaa cctttcttcc gccggcgcta aatagcgtac gatcccttgt tggtcaagaa
     7201 tcgccacacc ttctgtggaa ttttccacca gtgcgcggaa gtacgcttca cgttgctcca
     7261 atcgttcata taaataagca ttttcaatgg caaccgctac ttgatctgct aatgtgctgg
     7321 caaattgaat ttcctcttgc tggaagtggc ggctctcatc tgatgagaat aactctgcca
     7381 aacctagcga atatccacgt acccgtagcg gaatcaacaa cgatgagaac atcccatcat
     7441 ttttcagctg ttgaacttcg gcagggtcag cacctgcatc atccatacgg gtgaccagag
     7501 gcttcccagt ttgaagcaca tacaaggttg ccggatagtg ttctaatgaa aattcttttc
     7561 caatatctcc ctgttcgcta acatcatctt cggctgagta aatagcacga gtgatgagca
     7621 aattactttc agggagataa tctgaaatgg aacagaagtc cacgcgcatc atacgtgcaa
     7681 gagtagtggt tgcttcacga agaacgtcag ggagctgcaa gctggatgaa attgcacgag
     7741 taaccgataa taaggcactt aattcacttt ccctttttcg cgtcagtgca aacagggtcg
     7801 aattctcaat gatattcgcg gcatgacttg tcatggcatt agcaaggcga atctctgcct
     7861 gactaaattc tcgacgttta cggctctccc aaagttcgac cgtgccaagt aatcgtccgc
     7921 gagaatagat cggaacgaac atcatggttt cgatatgata atcaacaaat tgttgatatt
     7981 cttctgaggt caagttaggt tgcccagcat gaagtaccag agacctgcct tccatcattg
     8041 ccttcaaaac agtggcatag tcagcactgg ggtaatctct gccaaggtca gacttaagtt
     8101 cagcaggcgc agattctttg ccccagtatt ctgccaccac tttcattgac atcacttgta
     8161 aatcaaatga agtgatgtat gtgcttgtga cgtccaaggc ttctgccata tgacgcgcca
     8221 tttctttcag caatgccggc ggatccatca agctggcagt catatcttga gcagccgtgt
     8281 aaagctgttc taactctttt gccttggcac gcatctcgtc gtttagtttc actttaagaa
     8341 tggcgagagc aaactgattc atggactggt ccgctagttt cacctcttct gaggtaaata
     8401 catgttgatc actatagccc aaaactaggg caccaattcg catttggtct gcaattaatg
     8461 gaatacccat ggcagattgc acattaaact gttctgcaaa cgcagggtta atataaggcg
     8521 tatccatgac gttttgtatg ataagaatgc gcttttcctc caaaatagcg cgggtaaaaa
     8581 acttatcatc tggctctaaa agcggcggag tcacaaaaat atcgtgaaat ttttgagaaa
     8641 aaataaactt ggaaggaaca cctcgcgcct catcccacat cgcgaaatag tatccatcag
     8701 catctaaagc tttctcaagc ttttcaatga aatctctcaa caacgtatca aggtcaaatg
     8761 cagataaagc tgtttgtgta acctcattca atagagtctg ataccgagca atcgtatcca
     8821 gctttttaga tgcttcaaga ttttcaagag cataagataa atcacctgcc aactccacca
     8881 gcaaggaaat ctcttgttca tcaaaggctg ccgctagttc tgtatggtca acaaccagca
     8941 caccaaatat tttcgcatct cgctgtagag gtaaagagat cgcagaccta ttcgggaatt
     9001 tccctatgcg ggggcatttt tcacacgggt ctccgttacg gtctgatgga ttaacaataa
     9061 aaaatgaacg ggttcgaatc gcctgaatgg cacaggtcag cccgctctca aggtcgaggc
     9121 gagtagtgaa ttcgctttca tccacatcac ctcctgcatg agcaatatgc cgaagtgtga
     9181 ccatatcctc atccaacaaa cccacccatg caaaggagta cccttgccca gcaacaagaa
     9241 gctcacaggc gtcgtgaagc atcttttctg ggtcttgtgc tcgtacaata atctgattaa
     9301 tttcactgac cgtacgcaga accccgccaa ggcgctgctc acgttgtaaa gcttcttggg
     9361 tttctttcaa gctggcttca aaagattgta agaggtaagc tatcgaataa atcccagcga
     9421 cacttaatac aatcaacacc aacccaccac tgaaccatgc cccagggttg gcggcattca
     9481 cctgccattc tggcggaacc accatcagcc cagaaatttg cacataccca atgaccacta
     9541 atgttagcag agttacgaaa aaggcagcca aactatagcg tagattaaag aatatccctg
     9601 aaaagattac aaacgcaaat aaaagaactc tgccatcccc actgaacgaa gtgagcaaca
     9661 ttccgcttgt gcccaaagca tataaactaa aaagtagtat tcctgcacgc aggttcacat
     9721 tcaatctacg attgaatgtg acataaccaa ccaatgtggt cactatgccg taaaacacaa
     9781 taaccgaaat cgcaaccagt tttgggttat tcagtaggct ctgactttta ttgtatagat
     9841 caatgacgtt tcgaatgctt ccaataaatg ccaagaccca aaaaacgaaa attccaccca
     9901 agattaagtt caaaaggtca gcccgccatt catctaacga tgaaacaaac ttctcatttt
     9961 gaatcttggg catagcaaaa aatgcgtctt tgagtctttg atacataatg atttaggcta
    10021 tttttaaatt atagttgcat tttttgcctg aaacctttca gagttagtac ggttttgccc
    10081 aaaaacgccc cttctgcaag cctttttcat taaatattat gatatttatc acatggacag
    10141 gtggaaatac agacaggtat actacgccaa tctttttaga gaatttttgt ctgcaatcca
    10201 actcgaggag aaatcatgtc cgctagggat tctgttcctt cttctaaatt cgccatgttc
    10261 tggtcgttga ccaaaccctc gcaatctggt ctattacttg ctacgggtct ggctggctat
    10321 atgagtgctc gctgccctgt cttcaatctc tccaccatcc ttcagttatc cgtcagcttg
    10381 ttccttgcca ttgcaggcag caccctcctc aacatgtggt gggaccgtga catcgactct
    10441 aaaatggggc gtactcaaaa acgtgtcacc tccaaaggct tggttacaga taaagaagtc
    10501 ttggcagtcg gcatacttac atcggtcatt ggcattggtt gggcattagg catggactgg
    10561 ctctatggcg tggtcgtttt cgctggttgg ttcttcgatg tggttgtata tagtatgtgg
    10621 ctcaaacgtc gcacctgttg gagtattgtc tggggcggca tttcaggcgc catgcccatc
    10681 cttgcgggtc gtgcgcttgg tctcggctcc atcgactgga tcggcatcat gctcgcattt
    10741 ggcatcctgc tttggatccc cacccacacc ctcaccttca gcatgaaatt ccaaaaagat
    10801 tattcagatg caggcgtgcc taccttccca tccacatatg ggttagatat cacccgcttc
    10861 acgattgcat tctcgtccgt ccttgccgcc ggagcaatgg tcgccgcagg ggttggcatc
    10921 ggcatggact ggggcttcct cagattgctc ggcgtactct ctgcaggctt gctcgttcta
    10981 gcaaccgcta tgaccttcaa gcctaccgaa cttgttaact tcggtctttt caaatatgct
    11041 tccgtttaca tgctcgcagc aatgattctg gttgttcttg aagttatcta aatgaaactt
    11101 tcctcacgcg atcgcacttt attgttact
//
LOCUS       DAOQNJ010000016        10990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_2764_length_10990_cov_7.499223, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000016 DAOQNJ010000000
VERSION     DAOQNJ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2764_length_10990_cov_7.499223"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLG52_00990"
     CDS             <1..533
                     /locus_tag="PLG52_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB40040.1"
                     /translation="TRNIPVIICSIVEDIEKGFNLGASDYLVKPILEEDLVNSLDRLN
                     ADGSIREVLVIDDNPNDLRLIGKMLNDDGRYKPILAEGGRNGWTIISSGTPPHAIILD
                     LFMPEMDGIQILEQLQADKRLRDIPTIVISGMDVTPEQKNKLKEFGQRLLTKGAFNES
                     ELLTSIQRSLDRLSKK"
     gene            556..2211
                     /locus_tag="PLG52_00995"
     CDS             556..2211
                     /locus_tag="PLG52_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNB40041.1"
                     /translation="MSFIIKCLDCGHNAPYFPTSTTCPKCGSQWREAEYDYEMLGKTL
                     PEKLKSRQQDLWRYRELLPVRNPNISLSLGEGYTPLIRAVNLGMMLGCPNIFIKDERQ
                     GPTSSFKDRQASVTIAALKEAGITEMVAASTGNVAISYSAYASRAGMKLWAFVTSLVP
                     AVKMREIALYGSQVIKITGSYDQCKQVAAEFARQRKLYLDMGARTITSIEAMKTIAFE
                     IAEQLTNVLGPDENSQLRTPDWYVQAISGGMGPIGVHKGFREMQQMGWVDKIPAFAPI
                     QAEGCAPMVDSWKKGLAKAEPVTNPKTRIETLATGDPGRAYEFLKGHVDSSNGVFESV
                     TDEEAFRAMHVLAKMEGISAEPAAGAAFAGLFKLIRAGVIKPSDTVVVNCTGHTMPAE
                     QGILGENWARDVKFPSLAQAPQEEGLLSALTQVAPERFPKIAIVEDTLEARRLIRRIL
                     QSQGNFTILEAEDGRAGLDLVQKELPDLVILDLMMPEMDGFSVIEALRSNPETATIPV
                     IVATAKELTPDEKNRLSGHIQALLQKGDFLNDEFLDEVRSLLK"
     gene            2235..3182
                     /locus_tag="PLG52_01000"
     CDS             2235..3182
                     /locus_tag="PLG52_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB40042.1"
                     /translation="MAKKNTAGILAAFSSALFLGMAPVFGKLAITEGFSPFAVVALRT
                     GFAAGIMLLVMMLFYRQYLYIFPVGLIGCILAGAINGLGSLFYYLALERLDASLGQLL
                     YSLYPFFVALWLILDHQPPSRLTIFRLTLATIAVLLLTNVPDRPMDLTGVAMMIAAAV
                     LYAMHLPINQRVLYEVPAPTVALYTLISMSAIVIPAYLIFDRTWPASHAPWLPVAGLT
                     FVTIFSRVALFLGVKKIGGMQTALLGLAELLIAIFGSSLLLGESLTFTQWLGALGLAV
                     SLILVMYEKTAAFPAVSKSGWLSWIRPPGLPKDTFGPYE"
     gene            complement(3216..4115)
                     /locus_tag="PLG52_01005"
     CDS             complement(3216..4115)
                     /locus_tag="PLG52_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB40043.1"
                     /translation="MDKPTALIIEDDRDIVALFRHVLDVAGYQTEIVLDGKDAMDRLD
                     VMRPHIVLLDLQLPRVSGIDILKRMRDDERLMRVPVVVITAYAPYADSLPVEPDLLLL
                     KPVDINQLSNLVQRLQSTQGALDEPTHDFVTGLYTLSFFNVRMTFSLERIKQSSFRRF
                     GVLFADVSQMNDLRDYLDETEMRNFLRKLADQFRVTLRPTDTMAWSPEKSLFLTLIED
                     IPTPEAPLKIAGRLRDSMKRFIEQNDRGLGIRTNVGVLLCDAEYIDIQQVLNDIDLAR
                     QQLSAGLYTNPSIFDREMLNAHR"
     gene            4220..5545
                     /gene="yqeC"
                     /locus_tag="PLG52_01010"
     CDS             4220..5545
                     /gene="yqeC"
                     /locus_tag="PLG52_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HNB40044.1"
                     /translation="MDLIHALRLDQAKENPVVCFVGAGGKTTALFQLARQRIGKKNSL
                     VLVTATSHLGVWQIPLADHHVIVNKIEDLQLPSNGVVLVTGEIEKDRTLPVTGNALNW
                     LRAKCNSENVPLLIEADGSRQKPVKAPATHEPPIPDFADIVVYVCGISGIGKALNDEN
                     IHRPEIFSELTNLKMNEQVSAQSLISFLTHPQGGLKNIPAHANRIALLNQADTPELQS
                     MGGAIAQKLLPTFDAVVIGSLQNNVIHLVERTAGIILAAGSSSRYGQLKQLLDWKGKA
                     FVRHVAETALQAGLDPVVVVTGFRHADIAPHLQDLPVNIVYNPAYEQGQSTSIKAGLA
                     ALPKNTGAALFLLADQPQIPADVMRALIESHASERQSILAPLVLEEKRVNPVLFDRVT
                     FPDLMQLTGDIGGRGIFDKHKVSFLPWHDDILIFDVDKPEDYERLKNIE"
     gene            5542..6114
                     /locus_tag="PLG52_01015"
     CDS             5542..6114
                     /locus_tag="PLG52_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNB40045.1"
                     /translation="MKHISAIILAAGKSTRMGRQKMLMPWGSATVLEKVIQTVQAAEI
                     EDIILVTSPEIASKFQDKYLQVVLNAEDEMLKSIQLGLRAQKESAEATLICLGDQPQM
                     EEGSVRSVCEAFQKNAANLVVPSYQMRRGHPWLVERSLWSEILDIQNGTMREFLDKHA
                     NQIQYVNLSTPTILQDLDTPEDYLKYKPSM"
     gene            6149..7069
                     /locus_tag="PLG52_01020"
     CDS             6149..7069
                     /locus_tag="PLG52_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNB40046.1"
                     /translation="MRYFVIVNPTSGRGLGEKSIPQIESFLKEHGFDFTLVRTTGVWH
                     AADLAESAARDGYDVIVCASGDGTVNEAINGMMRAKKAGHTSAAFTVLSIGTGNDFAG
                     GTGIPTNLNDSLQALKANKRKKIDLGVVKGGDYPEGRYFGNGIGVGFDAAVGNEAIKV
                     RWTRGLLAYLIGVIKTVFLYYNPAQVEIILDDKETISQRSLMISVMNGRRMGGGFQMA
                     PESQPDDGLFDLCIAETASKGRILQMIPHFINGTQKTLPEIQMKRAKKVSIQSMDATF
                     PAHADGEFICLEGKNLTLEVLPQELEIIHA"
     gene            7076..7756
                     /locus_tag="PLG52_01025"
     CDS             7076..7756
                     /locus_tag="PLG52_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB40047.1"
                     /translation="MKKFSLWFITTALRVYFWLTLRVDAPGGMDKVPVKGPLIIITNH
                     TGQVEVPVFATMLQPRKITGWGKAEAFENPFLNWVFGAWDIIPVHRGEADMTALKSAF
                     RMLEEGRIFGVAPEGTRNYTGILRRGLPGAAILALKSGVPVLPIAHWGGEKYIKNLKS
                     FKRTDFHIRLGEPFYVKADGKVTSEMRQEIVDEMMYRIAAMMPKEYHGEYSDFGKSTE
                     KYLVNTNL"
     gene            complement(7791..8234)
                     /locus_tag="PLG52_01030"
     CDS             complement(7791..8234)
                     /locus_tag="PLG52_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40048.1"
                     /translation="MQIEIATRADDELYEAFQRLTPQLTQNNPPPTREDLSALINEPS
                     STLLLARNELGQVVGALNLTVYRVPTGIRAIIEDVIVDLSARGQGIGEAIMQQAIEIA
                     QQKGASNISLTSNPMRVAANKLYIKLGFEKRETNAYQMKFKKAAE"
     gene            complement(8224..8961)
                     /locus_tag="PLG52_01035"
     CDS             complement(8224..8961)
                     /locus_tag="PLG52_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNB40049.1"
                     /translation="MTTSAISETKPNFIRSILAWGVHLFTATGAIWGLLGIFAIFENN
                     YKMMIVWMIVAMLVDGFDGMLARWADVKKYANGIDGALLDNILDYLNYVLVPAIFMIH
                     TDLLPEPVKWLTACLILLTSAYQFTQTDAKTDDHHFKGFPSYWNVAALYMLLMNLPPW
                     VNFGFLMLFNIMVFIPVKYIYPSRNSYLRTLTLAFTYLYGAVGIWGLLLYPNTPSWIV
                     WASFLYVGYYLVLSLIPKSKNTTAHAN"
     gene            9100..9600
                     /locus_tag="PLG52_01040"
     CDS             9100..9600
                     /locus_tag="PLG52_01040"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNB40050.1"
                     /translation="MKLFVSLLFFLILAVVFFWQSYRLLSRSLPGLRDAQASRSWPSV
                     QGTITIVSSRMEWVNIKRKNLSYFRPWVQSTYTVNGQALVCERFAFNDHLISASTKKA
                     AEDHVKSYQIGQSVQVYYDPNDPQKSVLEPGNLSPSMPSLLGGVVFFILALLQFVMAF
                     LAIKQA"
     gene            complement(9686..10123)
                     /locus_tag="PLG52_01045"
     CDS             complement(9686..10123)
                     /locus_tag="PLG52_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40051.1"
                     /translation="MPIIHKIDPEHGLLYILLHGRVAMADVVTYEKLLLDDPLRQPNL
                     KILADLLLADMEVDLDSLKKISARHRSLHESGWKMEQTAVLTNNRMLELLGDAYELMS
                     FGVPVHFKIFSVLRDAILWLELRQSQEQIKSIRQQLVDRVSSS"
     gene            complement(10202..>10990)
                     /locus_tag="PLG52_01050"
     CDS             complement(10202..>10990)
                     /locus_tag="PLG52_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNB40052.1"
                     /translation="AEWPAVMEEFIDESLNREMELVMKLVSLGHSARQKANRKVRQPL
                     AEAAFSVGNNTERAALLKNAEVVQDELNVKQVRLLDASTEAVSHTVKPLPKQLGQKYG
                     NKFPVIQKAILAMNAEEVAKALLSGNPLEVNAGGETFHIISEEVEVKAMAKEGFAVAE
                     DGAYVAALVTTLTPELVAEGLAREFVRRVQDLRKTANLDVADRIELFVEASAGLKTAV
                     EAHADYIKAETLTVKLSFAAAPANASVVEDEFEGEKVKVGLLKN"
BASE COUNT         2958 a         2577 c         2610 g         2845 t
ORIGIN      
        1 caacccggaa tattccggtt atcatatgta gtatcgttga agatattgaa aaaggtttca
       61 accttggcgc ttccgattac ttggtaaagc ctattctaga agaagacttg gtcaattcgt
      121 tagatcgctt gaatgcggat ggctccattc gtgaagtact tgtgattgat gacaacccca
      181 atgacctgcg tttgattggc aaaatgctga atgacgatgg acgatataaa cccatccttg
      241 ccgaaggtgg gcgcaacggt tggacgatta tctcctctgg tacaccgcct catgccatca
      301 tcttggattt gttcatgcca gaaatggacg ggattcaaat ccttgagcag ttacaggcag
      361 ataagcgttt acgtgatata cccaccattg taatcagcgg tatggatgtc acgcctgaac
      421 aaaagaataa gctcaaagag tttggtcaac gcttattaac caagggagct tttaacgaaa
      481 gtgaattact aacttcgatt caacgctcgc tggatcgctt gtcaaagaaa taatcgaagt
      541 gtacaggaga attaagtgtc atttattatt aagtgcctag attgtgggca taacgcgcca
      601 tactttccaa cttccacaac ctgccccaag tgtggaagtc aatggcgcga agcggaatac
      661 gactatgaaa tgcttggcaa aaccttgcca gaaaagttaa agtcgcgcca gcaagacctt
      721 tggcggtata gagagctact tcctgttcgc aacccaaaca ttagtttatc gctaggtgaa
      781 ggctacaccc cattgattcg tgccgtcaac ttgggaatga tgttgggctg tccaaatatc
      841 tttatcaaag atgaacgaca aggacccact tcttctttca aagaccgtca ggcatcagtg
      901 actattgcgg cactcaaaga agccggtatc actgaaatgg tagctgcatc gactgggaat
      961 gttgccattt catattctgc ctacgcttct cgagcaggga tgaagctttg ggcatttgtt
     1021 accagccttg ttccagcagt caaaatgcgt gagatcgctt tgtatggcag ccaagtcatc
     1081 aaaatcactg ggtcatatga ccaatgcaaa caagtggctg ctgaatttgc acgtcaacgc
     1141 aaactgtatc tggacatggg tgcacgtact atcacatcca ttgaagcgat gaagaccatt
     1201 gcatttgaaa tcgcagaaca gcttaccaac gttctcggtc ccgatgaaaa ctctcagctg
     1261 cgcaccccgg attggtatgt gcaagccatt agcggcggga tgggaccgat tggcgtccac
     1321 aaaggctttc gtgaaatgca acaaatgggc tgggtggaca aaatccccgc ttttgctccc
     1381 attcaagcag agggctgtgc gcccatggta gatagttgga aaaagggtct tgcaaaggca
     1441 gaaccggtta ccaacccaaa aactcgcatt gaaacccttg ccacaggcga ccccggacgt
     1501 gcctacgaat ttttaaaagg gcatgttgat tcaagtaacg gtgttttcga aagtgtgacc
     1561 gatgaagaag ctttccgtgc tatgcacgtg ctcgcgaaga tggaaggtat ttccgcagag
     1621 cctgccgctg gagctgcgtt tgctggcttg tttaaattaa ttcgtgcagg tgtgatcaag
     1681 ccaagtgaca cggtggttgt taactgcact gggcatacca tgccagctga acaaggtatt
     1741 cttggtgaga actgggcacg agatgtgaag ttcccgtctt tagctcaggc tccacaagaa
     1801 gaaggcttac tatctgcgtt aacgcaagta gcacctgaac gcttccccaa gatcgccatc
     1861 gtagaagaca cgcttgaagc acgccgcttg attcgccgca ttttgcaatc gcaagggaat
     1921 ttcacaattc ttgaagcaga agatgggcgt gccgggttag accttgttca aaaagaattg
     1981 cccgaccttg ttattctcga cttgatgatg ccagagatgg acgggttctc tgttatcgaa
     2041 gcattacgtt cgaatcctga aaccgcaacc atccctgtga ttgttgcgac ggcaaaagag
     2101 ttgacacccg atgaaaagaa tcggctcagc ggacacatcc aggcattgct gcaaaaaggt
     2161 gattttctca acgacgaatt tctggacgag gtcaggtctc tcttaaagta actattcaat
     2221 aaaggctggc aagattggca aaaaaaaata cagccgggat ccttgcggcg tttagctccg
     2281 cccttttcct tggcatggca cctgtgttcg gtaaacttgc catcacagaa ggtttttcac
     2341 catttgcggt tgttgcttta cgaactggat ttgcagcggg gatcatgtta ctggtcatga
     2401 tgttgttcta tcgccagtat ctctacatct ttcctgtagg tctaatcggc tgtatccttg
     2461 ctggtgccat taatggacta gggtcgctgt tttactacct tgccttggaa cgtttagacg
     2521 ccagcttggg gcaactgctt tattctctct atccgttctt tgttgcactt tggttgattc
     2581 ttgaccatca gccgcccagc cgtctgacaa ttttccgcct cacacttgca accatagctg
     2641 ttctgctcct tacgaatgtt ccagaccgcc ccatggacct aaccggcgta gctatgatga
     2701 tcgcagccgc tgtgctatat gctatgcatc tcccaattaa tcaacgggtg ctctacgaag
     2761 tccctgcgcc gacagttgcg ctctacacac tgatatctat gagcgcgatt gtgatccctg
     2821 cttacttaat ctttgaccgc acttggccag catcgcatgc gccttggcta ccagtcgcag
     2881 ggctaacctt tgtgaccatt ttctcgcgtg tggctttatt ccttggcgtg aaaaagatcg
     2941 gtggcatgca aaccgcattg ctaggtttgg cagagttact gatcgctatt tttggtagca
     3001 gtttactgct tggagaaagc cttacattta cccaatggct tggtgcgctt gggctggcag
     3061 tgagtcttat ccttgtaatg tatgaaaaaa ccgctgcatt cccagccgtt tccaaaagcg
     3121 gatggctctc atggattcgc ccacccgggt tacctaaaga cacatttggt ccatatgaat
     3181 aaaaagaggt cgacgtcatg tcgacctctt tttattcagc gatgtgcgtt caacatttct
     3241 ctgtcaaaaa ttgatggatt ggtatataac cccgcactca gttgctgacg tgccaagtcg
     3301 atatcgttca aaacttgctg aatgtctata tattcggcat cacacaacaa aacacctaca
     3361 tttgtacgaa ttcctaatcc gcggtcattt tgttcgatga aacgcttcat gctatcacgc
     3421 aaacgcccag caatctttag tggagcttca ggcgtaggaa tgtcttcgat caaggtcaag
     3481 aaaaggcttt tttctggaga ccaagccatg gtgtcggtag gacgaagcgt cacgcggaat
     3541 tggtctgcca gtttgcgcaa gaaattgcgc atctctgttt catccagata atctcgcaga
     3601 tcattcattt gagagacatc cgcaaaaagc accccaaaac ggcggaaaga actttgcttg
     3661 atgcgctcta atgaaaaggt catacgcaca ttgaaaaatg aaagcgtata tagaccagtt
     3721 acaaaatcat gggtaggttc atcgagagca ccctgtgtgg actgcaatcg ttgcaccaag
     3781 ttactaagtt gattgatatc tactggcttc agaagcagaa gatcgggctc aaccggaaga
     3841 ctatctgcat aaggggcgta ggctgtaatc accaccacag gtacacgcat gagacgttca
     3901 tcgtcgcgca tgcgctttag aatatcaata cctgaaactc ttggtaattg cagatccaac
     3961 aaaacgatat gcggacgcat tacatccaaa cgatccatgg catctttacc atccagaacg
     4021 atctctgttt ggtaaccggc aacatctaac acatgccgaa acaacgcaac aatatcgcgg
     4081 tcatcttcaa taattaaagc agtgggttta tccataagtt cgcccttctt cgcaatatag
     4141 atattatacg accttcaagc accgttcagg gtgtcatttc aggtttttta tcatacaatt
     4201 acaacgaaag ctaatccata tggacttaat acacgccctc cgtcttgacc aagccaaaga
     4261 aaatccagtt gtttgttttg tgggtgccgg tggcaaaacc actgccctct ttcagctggc
     4321 acgtcaacga ataggaaaaa agaattcgct tgtgcttgta acagccacca gccatttggg
     4381 tgtgtggcaa attccgcttg cagaccatca tgtaatagtg aataaaatag aagatttaca
     4441 attaccttca aatggagtcg ttctagtcac aggtgaaata gagaaagaca gaaccttacc
     4501 agtaacaggc aatgccctaa attggctacg cgcaaaatgc aattctgaga atgtccctct
     4561 tctcatcgaa gcagatgggt cacgtcaaaa accggtcaaa gcacctgcca cgcatgaacc
     4621 gcctattcct gatttcgctg acatcgtggt ttatgtttgc ggtataagcg gcatcggtaa
     4681 agcgctaaat gatgaaaaca ttcaccgccc tgaaatcttt tcagagctaa caaacctcaa
     4741 aatgaacgag caagtctcag cgcaatctct tatttcattc cttacccatc ctcaaggtgg
     4801 gttgaaaaat attcctgcac atgcaaatcg aatcgcacta cttaatcaag ccgatactcc
     4861 agagttgcag tctatgggtg gagcgatagc gcagaaactg ttaccaacat ttgatgctgt
     4921 tgttattggc tcgcttcaaa ataacgtcat acatctcgta gaacgaacag caggaattat
     4981 ccttgcagcc gggtcatcca gccgatatgg tcagcttaaa cagcttttgg attggaaagg
     5041 caaggcgttt gttcgacatg tagcagaaac cgcgcttcaa gccgggctgg accctgtagt
     5101 ggtcgttaca ggctttcgcc acgccgacat cgcccctcac ctacaagacc tgccagtaaa
     5161 tattgtctac aaccccgcat acgaacaggg acaaagcact tccataaaag caggtcttgc
     5221 agcgttaccg aaaaacacgg gcgcagcttt atttctctta gccgaccagc cgcaaattcc
     5281 agcggatgtg atgcgggcgt tgattgagtc tcatgctagt gaaaggcagt ccattctggc
     5341 tccgcttgtg ttggaggaaa aacgcgtaaa cccagttctc tttgatcgcg ttaccttccc
     5401 agacttaatg caactaacag gtgacattgg cgggcgcggc atatttgata aacacaaagt
     5461 ttccttctta ccttggcatg atgatatttt gatcttcgat gtggataagc ccgaagatta
     5521 tgagcggttg aaaaatatag aatgaagcat atctcggcga ttatccttgc agcaggaaag
     5581 tctactcgta tggggcgaca gaaaatgctc atgccttggg gaagcgctac tgtactggaa
     5641 aaagtaatcc aaaccgttca ggcagcagaa atcgaagata ttattttggt tactagcccc
     5701 gaaatcgcat caaaatttca agataaatac ttgcaggtag tgcttaatgc agaagacgaa
     5761 atgctgaaat cgattcagct tggattacga gcacaaaagg aatcagcgga ggcaacattg
     5821 atttgtcttg gcgaccagcc ccaaatggaa gaaggaagcg tgcgaagcgt atgcgaagca
     5881 ttccaaaaaa atgctgcaaa ccttgtagtg ccaagctatc aaatgcggag agggcatccg
     5941 tggttggtag agagaagttt atggagtgaa attctggaca tacaaaatgg aacgatgcga
     6001 gagtttttag ataagcatgc aaatcagatc cagtatgtga atttaagcac acccacgatt
     6061 ttgcaggacc tcgatacacc cgaagattac ttgaaatata aaccatccat gtgataaact
     6121 ccctccactt ttcacactgg gagcaggcat gagatacttt gtgatcgtaa atccgacttc
     6181 ggggcgtggg cttggggaaa aatccatacc acagattgaa tcatttctaa aagagcacgg
     6241 ctttgatttc accttagtgc gtacaactgg ggtttggcat gcagcagact tggctgaaag
     6301 tgccgcgcga gatggctatg acgtgatcgt ttgtgccagc ggagatggca cggtgaatga
     6361 agccatcaac ggtatgatgc gtgccaaaaa agctgggcat actagtgcgg cattcacggt
     6421 gttgagcatt ggcacaggga atgactttgc aggcggaaca ggtattccta caaatttgaa
     6481 tgatagcctg caagccttga aggcaaacaa gcgaaagaaa atagacttgg gcgtagtgaa
     6541 aggcggagat tacccagagg gtcgttattt tgggaatggc attggggttg ggtttgatgc
     6601 ggcagttgga aatgaagcga tcaaagtacg ttggacacgc ggcttgttgg catatctcat
     6661 tggtgtgatc aaaacggttt ttctttatta caaccctgcg caagtcgaaa ttattttgga
     6721 tgataaagag actatcagcc aacgttcgtt gatgatatcc gtgatgaacg gacgacgcat
     6781 gggcggcggt tttcagatgg cgcccgaatc tcagccggac gatggtttgt tcgacctttg
     6841 catcgcagag actgcttcga agggacggat tttgcaaatg attccccact ttattaatgg
     6901 aacgcaaaag accttgcccg agattcaaat gaaacgagcc aagaaggtat cgatccagtc
     6961 tatggatgcc accttcccgg ctcatgctga tggagaattt atctgcctcg aagggaagaa
     7021 tttaaccctt gaggttttgc cacaggaact tgagatcatt cacgcttaat atcttatgaa
     7081 aaaattttct ctttggttta ttacaacagc tcttcgtgtt tacttctggc ttaccctgcg
     7141 cgttgacgcc cccggtggca tggacaaagt ccctgtcaaa ggaccgttaa tcatcatcac
     7201 caatcacacc gggcaggtgg aagtgcccgt tttcgcgacc atgctccagc cacgaaagat
     7261 caccggttgg ggaaaagcag aagcttttga aaacccattt ttgaattggg tatttggcgc
     7321 atgggatatc atccctgttc atcgcggtga agcggatatg accgcactta aatctgcttt
     7381 tcgtatgctg gaagaaggtc gcatcttcgg cgttgcccct gaagggacac gtaactacac
     7441 cggcattcta cgccgcgggt tgccaggtgc tgccattttg gcattgaaat ctggcgtgcc
     7501 ggttttgccc attgctcatt ggggtggcga gaaatatatc aaaaacctaa agagcttcaa
     7561 gcggacagat tttcacattc gccttggcga gcctttctat gtgaaggcag atggcaaagt
     7621 gaccagtgaa atgcgccaag agatcgtaga cgaaatgatg tacaggatcg ccgccatgat
     7681 gccgaaagaa tatcacggcg aatatagtga tttcggtaaa tcaacggaga aatatctggt
     7741 caatacaaat ctataaataa aaaccggctc atagtagagc cggtttttat ttattcagca
     7801 gctttcttga atttcatttg ataggcattt gtttcgcgtt tttcaaagcc caacttaata
     7861 tacaatttgt ttgccgccac tcgcattgga tttgaggtta gcgagatatt gctggcgcct
     7921 ttttgttgcg caatctcaat tgcttgttgc atgatggctt caccaatccc ctgcccccga
     7981 gcagacaagt ccacgatgac atcttcgatg atggcgcgga tgccagttgg cacgcgataa
     8041 acggtaaggt ttagggcgcc gacgacctgt ccgagttcgt tgcgggcaag cagcagcgtg
     8101 gacgaaggtt cattgataag cgcagacagg tcttcgcgcg taggaggcgg attattttga
     8161 gtcaactgcg gcgtgagacg ttgaaacgcc tcatagagtt catcatcggc tcgggtggcg
     8221 atttcaattt gcatgggcgg ttgtgttctt gctctttgga attaggctca acacgagata
     8281 atagcctaca tagagaaagg atgcccagac gatccaagat ggggtattgg gataaagcag
     8341 caaaccccag atacccacag cgccatagag atacgtgaac gccagtgtga gggtacggag
     8401 ataactattg cggctggggt aaatatactt gaccgggatg aaaaccatga tgttgaagag
     8461 catcagaaaa ccgaaattga cccagggcgg caaattcata agcagcatat acagcgcagc
     8521 aacattccaa taggaaggga aacctttgaa gtgatggtca tcggtcttgg catcggtttg
     8581 ggtgaattga taggcggacg tgagcaggat gagacaagcc gtaagccatt tgactggctc
     8641 gggcagcaga tcggtgtgga tcatgaaaat ggcagggacg aggacatagt tgagatagtc
     8701 aaggatgtta tcgagcagag caccatcgat accattggca tattttttca cgtctgccca
     8761 gcgcgcgagc atgccatcga agccatccac gagcatggca acgatcatcc agacgatcat
     8821 catcttgtag ttattctcaa agatggcaaa gatgccaagc aagccccaga tagcccccgt
     8881 agcagtaaag aggtgcactc cccaggcaag gatggagcgt atgaagttag gtttggtttc
     8941 agaaatcgca gatgtggtca tatccagtcc tttagatagt ttcagtattt aacactagag
     9001 attataaaag gaaactgttt tttcagaata aagattagaa ggtgtaaggt aaaatatttt
     9061 cactctgcag aattgctaaa aggacgtcat ttattccaca tgaaactatt tgtctccctc
     9121 ctctttttcc tgatcctcgc cgtggttttc ttttggcaaa gttaccgtct gcttagtcgc
     9181 agcctaccgg ggctgcgtga tgcgcaagcc agccggtcgt ggccttcagt acaaggaaca
     9241 atcactatcg tctcctcccg catggagtgg gtcaatatca aacgcaagaa tctatcgtac
     9301 tttcgtccgt gggtacaaag tacatataca gtcaatgggc aagccctagt ctgtgagcgg
     9361 tttgccttca acgaccatct gatctctgcc agcacaaaga aagctgcgga agaccatgtc
     9421 aaatcctatc agatcgggca aagtgttcag gtctattacg accctaacga cccgcaaaag
     9481 tctgtgctgg aaccggggaa tttatctcct tctatgccat cactgttagg tggggtcgtt
     9541 tttttcatcc ttgccctact ccagtttgtg atggcctttt tagcgataaa acaagcttga
     9601 tttagaattt ttaaacaaag acctccgaga ttttataaaa tctcggaggt ctttttactt
     9661 acagccacta ggtctaaaac cttttttacg aagaagaaac acgatctacc aattgctgcc
     9721 gaatggattt gatctgttcc tgtgattgcc ttaactccag ccataaaata gcatcacgta
     9781 gtacggagaa aatcttgaaa tgcacaggca ccccaaaaga catcaactca taggcatcgc
     9841 cgagcagttc tagcatgcgg ttatttgtga gtacagccgt ctgctccatt ttccagccag
     9901 attcgtgcaa gctacgatgc cgtgcactta tctttttcaa actatccagg tctacttcca
     9961 tatccgctaa caaaaggtct gccagaatct tcaaattcgg ctgccgtaaa gggtcatcta
    10021 gcaataactt ttcgtaggtc accacgtctg ccatggcaac tctaccgtgc aataagatat
    10081 aaagaagccc atgttcaggg tcaatcttat gaataatcgg catcttaggt catcctattt
    10141 caaaacaagt taatcccctc ggtaaaaact cactcacccg aggggattaa cttttcaagc
    10201 actaattttt caacaacccc actttcactt tctcgccttc aaactcatct tccaccactg
    10261 aagcatttgc aggtgcggca gcgaaggata acttaaccgt cagcgtttcg gctttgatgt
    10321 aatccgcatg tgcttccaca gcagttttca agccagcgct ggcttccacg aagagttcga
    10381 tgcggtcggc aacgtcgaga tttgcggtct tgcgcaagtc ttgcacgcgg cgcacaaatt
    10441 cacgtgccag cccttcggca acgagttcag gagtcaacgt ggtgacgagc gccgccacat
    10501 acgcgccatc ttcggcgacg gcaaagcctt ccttcgccat ggctttgact tccacttctt
    10561 cagagatgat gtggaaagtc tcgcctccgg cattgacctc aagcgggttg ccgctcaaaa
    10621 gggcttttgc cacttcctcg gcattcatcg ccaaaatcgc cttctgaata accgggaact
    10681 tattgccata cttttgcccg agttgcttgg gcagcggctt gacggtatga gacacagctt
    10741 ccgtcgaggc atccaacaag cggacttgct tgacgttcaa ctcgtcctga accacttctg
    10801 cgttcttgag caatgcggct cgttcggtgt tgttacccac cgagaaggcg gcttctgcca
    10861 agggctgacg tactttgcgg ttcgccttct gacgcgccga gtgaccgagc gagaccagct
    10921 tcatgaccaa ttccatttca cggttgagtg actcgtcgat gaactcttcc atcacggcgg
    10981 gccattcagc
//
LOCUS       DAOQNJ010000017        10843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_2824_length_10843_cov_7.704579, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000017 DAOQNJ010000000
VERSION     DAOQNJ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_10843_cov_7.704579"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="PLG52_01055"
     CDS             complement(<1..1417)
                     /locus_tag="PLG52_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40053.1"
                     /translation="MKGSIIPLRRWISAAIVLTLVGQACTISLFDPSSGNTNSGTPAP
                     AIATNTPYAATQTTFIVTLPEPLQAGDTLAISVVDEVTGIRFNETKYPMTARDSLTYT
                     ASLPVPVNSVIKYRYVRLGGSGAVEDTTFGTAIRYRLYSAASSAEVQDTVADWSDRTY
                     SRPVGTILGQVYNADTGSPIPNVLVTAGGIQYITDSLGRFEISGLPVGTHQLVAYSLD
                     GLYVPFQQGAIVAESNPTIVDLRIKPTHLVNVTFFVTVPEDTVPGAPVRIAGNILQLG
                     NTFADLQGGVSTVTDRMPVMSLQPDGRYSVTLGLPAGTYIQYKYTLGDGFWNAEHKSD
                     GNWIVREFLVPSQDITIQDTVSSWLASNQSGPILFEVSVPSVTLPSDIVYMQFKSAGW
                     MEPIPMWPLGNNRWAYKLYGPLNFLGSFSYRYCRNGQCNSADDNQTIGETTAGRTAST
                     SLLGQDIQDSVGSWKWFENPES"
     gene            complement(1460..2377)
                     /gene="tatC"
                     /locus_tag="PLG52_01060"
     CDS             complement(1460..2377)
                     /gene="tatC"
                     /locus_tag="PLG52_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNB40054.1"
                     /translation="MRKFFKGVWWVITLPFRLVYAIITFPFRAGYRFFKLLNSEPEER
                     PIADVFADLVTEKEVRDQLWEQIEAFRMHLLRSIIALSILVIAAFWVAEPAMAFLSEP
                     VGGLEKLQAIEVTEEIGVFMRVAMTIGIAIAFPYIAFEFWLFAAPGLKPREKKLGLVG
                     IPLATLLFLLGMAFTFYVLLPTALPFLGSFTSISQFWSAREYFSFVTGLMLWIGGFFE
                     FPLLIYVLTAIGLVNPKFLADQWRLAIVIIAVLAAAITPTVDPVNMGLVMVPMILLYF
                     ISIGLSYLAYAGRSKNAEVQEAASDVELG"
     gene            complement(2370..2777)
                     /locus_tag="PLG52_01065"
     CDS             complement(2370..2777)
                     /locus_tag="PLG52_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNB40055.1"
                     /translation="MEILGIGMPELIFVVIIALLILGPKDMQKAGKTIGKFLRDIITS
                     DGWKLFQQTSRDLRTLPNRLIREANEDLDKVNREIKSAASTPDPARSAPANSRPQPQP
                     YSATPPIKPGGENVILPNPQEKNNPESETPNNA"
     gene            complement(2780..3238)
                     /gene="nusB"
                     /locus_tag="PLG52_01070"
     CDS             complement(2780..3238)
                     /gene="nusB"
                     /locus_tag="PLG52_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNB40056.1"
                     /translation="MKPRTRARALALQVLYETDLSTHLPGDVLKTRLEETTLPQDLAE
                     FARSIIFGILPIRENLDRVIAKYAPEWPLDQIAAIDRNILRMACWEFAISRQTPVKVA
                     INEAVELAKLFGSDSTARFVNGVLGTLVEHEQEIYQVLKNEIPQETNLES"
     gene            complement(3243..3590)
                     /locus_tag="PLG52_01075"
     CDS             complement(3243..3590)
                     /locus_tag="PLG52_01075"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNB40057.1"
                     /translation="MTESTQGKTTVSPEVLTTIASLAALEVPGVSRLAPVSGGVNRLF
                     RRGTSDGVRIEAKDNTVFVDLFLILKDGVNIREVGRNVQANVARSVQEMVGMEVGEVN
                     VHIEDIDFEGDEA"
     gene            complement(3604..4362)
                     /gene="fabG"
                     /locus_tag="PLG52_01080"
     CDS             complement(3604..4362)
                     /gene="fabG"
                     /locus_tag="PLG52_01080"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNB40058.1"
                     /translation="MSFKLSLENKVAIVTGGSRGIGRAIALEFADRGAAVVVNYNKSP
                     ESAEEVVKKIQEAGGKAAAFQADVSDFKQAEALVKFAIDTFGDLHILVNNAGITRDQL
                     IMMMPEADWDAVINTNLKSTFNCSKAAVKHMMRKRTGRIINMASVAGQMGNPGQTNYS
                     ASKGGQIAFTKALAREVSARNITVNAIAPGFVDTEILDAMTPEALEAALKLVPLGRKG
                     KPEEIAFTATFLASDGAAYITGQVIGVDGGMAMM"
     gene            complement(4384..5244)
                     /locus_tag="PLG52_01085"
     CDS             complement(4384..5244)
                     /locus_tag="PLG52_01085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40059.1"
                     /translation="MPLTNDQCLELFGSSQIDLIRMADAHTLNVQAWLSSRSPMAKTF
                     QGMGVRASSTGLKLPLLNLALGANFPDKTASSEIEAEVKQVIDFFTERNVPWYWWMNA
                     EPRPANIATILEKFGCVYDAPPLPAMIAPTKQDASSFPQVDKSIQVWRAASIKDLQMA
                     STIRRRAFQFTEGEALTYFEDMAQDWLSNESSARLFLAGPKKGEAVSIGAIIEAKGIP
                     GIYVMATLPEFHRQGYGKAILSRLLSEAAQCEGKIVALTASSAGAGLYAQFGFRHLFD
                     FNFYSIPDEV"
     gene            complement(5367..6041)
                     /locus_tag="PLG52_01090"
     CDS             complement(5367..6041)
                     /locus_tag="PLG52_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HNB40060.1"
                     /translation="MKLPTLLSYLVSLATPLVLIGLALRILLTPLYYTVEYNMPYFPA
                     DEYGFTKEDRLKWAKPSVEYLVNSADISYLGDLKFDNGTPIYGEAELSHMHDVKNVVQ
                     GALKVWYLSIAVLILLAILAQRNGWMPLYLTGLRRGGWWMIGLAVTLGFIAGAGILLN
                     PDIFWNFFAWFHSLFFEGDSWLFYYSDTLIRLFPIRFWQDAVIAMAVIALGGGLGLAF
                     GLKTTN"
     gene            complement(6041..7594)
                     /locus_tag="PLG52_01095"
     CDS             complement(6041..7594)
                     /locus_tag="PLG52_01095"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNB40061.1"
                     /translation="MKRYLLFTVFISGMTTLAAELAAGRLIGNVFGTSNLVWASIIGL
                     ILIYLTLGYFLGGKWADKDPSPLAMYRILAWGAFTVGLVPYVAVPVLRSAASAFEALS
                     VGIMAGSFIVVLILFIVPITLLGAISPYAIRLSVDDASKAGQISGQIYAVSTLGSFIG
                     TFLPTLVFIPTVGTRLTFAIFGMTLLVVALVGLARFASTREMLKLIWMPILLAVIAFI
                     FSSSALKTNKGQVYETESEYNYIQVQESNGYTLLRLNDGQGIHSIYHPENLFYNGPWE
                     LFMVGPYFYENRSPDDIKEMAIIGLAAGTAARQATIVYGEDLHIDGYELDPKIAEVGY
                     EYFHMGSPNLNVILGDGRLNLDRSTKKYDIIAVDAYRPPYIPPHMTTLEFFEICASHL
                     ADNGVLTLNVGSTPGDRRLIDGLATTMAQVFPSIHIMDIPGTLNTMIYATKQPTTADA
                     FLANTLRIAEDKSLNPLLVTTMSSAYANLKPGYETTTVFTDDLAPIEWIVNDMVVKFV
                     LEGGLEFLQ"
     gene            complement(7685..9487)
                     /locus_tag="PLG52_01100"
     CDS             complement(7685..9487)
                     /locus_tag="PLG52_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNB40062.1"
                     /translation="MKTKSTNPLSLFFNLTVLAAYFHALMEWLFFVTQSSSLSTLSLL
                     DKIEVLFSTGGVIVLLLLALLVVFAVPALKWKFFGYVPAALMVSVTALVMIDNFTYTV
                     FKFGIVSTSGAWRLLYTVAFVLLFYWVFRFVERQKLWKGVSFLSLGLLGVSTVVILSI
                     YNSRSPYMSDFTPEATEASAEHPNILILGSDGLSASYLSAYGFEGNTTPFIAELTQTS
                     LVAENAFPNVSSTTGSTTSALTGKMPAEVQVFRYPDILSKEDSFEHLPGILKHMGYKT
                     VELGTTYYVDARKLNLADGFELVNNVSVDNPILDAIQPLLGNSPSAFFLQTVTERASD
                     RLFHIFFIKEMENPFLQVNNPNARMTDEERTQQIINLLETSDRPVFVFSHYMNTHGPL
                     FSTDDKHVEENEDSAESEKEWDVELYKKAIRTFDDHVREIYTYLEESGKLDNTIIVIY
                     TDHGYRYVVNQRIPIIFHFPNNDHAGTIQNNTQIIDIPATLLDYINVDQPEWMTGSSL
                     LLGEPPAMREIISTTAGSPKKIKPPFYQIKSLQFIVCQKWYSWNVQDNEFKTGEVKSH
                     TAKCESNLLPSDDEIRQRMIEYFKKYNYDTSSLE"
     gene            complement(9527..10141)
                     /locus_tag="PLG52_01105"
     CDS             complement(9527..10141)
                     /locus_tag="PLG52_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB40063.1"
                     /translation="MRFFFNLLYHPFAFTYDLVAWVVSFGKWNDWVRSISPHLEGTRL
                     LELGHGPGHLQRFLLDLGLNPVALDESTQMGQIAKRRLGSKQKLTRGLAQNLPFAKAS
                     FETVISTFPSEYIFDPKTLAEIKRVLVNEGKLIVLPAAFPSNGFLKWLYKVTGESPEA
                     LDDALKERLKSPFVSAGFNVEIQMIEMKSSTLVLILARSIFHNS"
     gene            complement(10211..10591)
                     /locus_tag="PLG52_01110"
     CDS             complement(10211..10591)
                     /locus_tag="PLG52_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNB40064.1"
                     /translation="MSDRDEFGAFLVGFVVGGLTGAVVALLFAPQSGEETRALIKDKS
                     IELRDKAQVSAEEAYARAEAAAKEARARAEELAKEARTRAEELTKEARTRAEQLASEV
                     KERGKGAIEAVRKPKKDGSEATPI"
     gene            complement(10627..>10843)
                     /locus_tag="PLG52_01115"
     CDS             complement(10627..>10843)
                     /locus_tag="PLG52_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40065.1"
                     /translation="ALIVLVIQLASLINLLQNEVRPILQATSETVNTLRGTAEFLGEN
                     FVEPVIKLNGYLAGLKRMLELLGVKPK"
BASE COUNT         2931 a         2481 c         2712 g         2719 t
ORIGIN      
        1 aactttctgg gttttcaaac catttccacg aaccaacact atcctgaata tcctgtccaa
       61 gcaagctggt gctggctgtt ctccctgctg tggtttcacc tatggtttga ttgtcatctg
      121 cgctattgca ttgaccgttg cggcagtagc gataagagaa agagccgagg aaattgagcg
      181 gaccatataa tttatatgcc cagcgattgt ttccaagcgg ccacatgggg atgggttcca
      241 tccacccagc agatttgaat tgcatataca caatgtcgct aggtagcgtg acagaaggaa
      301 cacttacttc aaataatata ggaccagact gattagatgc tagccatgaa gatacagtgt
      361 cttgaatagt tatatcttgg ctgggaacca aaaactcacg cacgatccaa ttaccatctg
      421 atttatgttc tgcattccaa aagccatcgc ccaaggtgta tttgtattga atataagtcc
      481 ctgctggcaa gcccagtgtg acagaatagc gcccatccgg ttgcagactc atgacaggca
      541 tacggtctgt taccgtgctc acgccgcctt gtaaatccgc gaaggtattg cccaattgca
      601 gaatgtttcc tgcaatgcgt acaggtgcgc ctggaacagt atcttcaggg acagtaacga
      661 aaaaggttac attgacaaga tgtgtgggct tgatgcgcaa atccacaatg gtgggattgc
      721 tttcggcaac gatggcacct tgttgaaaag gaacatacaa tccatcgagg ctgtatgcta
      781 ccaattgatg cgtccctaca ggtaagcctg aaatttcaaa gcgtcctaaa gagtcggtga
      841 tgtactgaat gccgcctgca gtgactaaaa catttgggat gggcgagccg gtatctgcgt
      901 tataaacttg accaagaatt gttccaactg ggcgagaata agttctatcg ctccaatctg
      961 caactgtatc ttgcacttcc gcagaactag cagcactata caaccgatac cgaatagccg
     1021 ttccaaatgt cgtatcttcg acagcacctg aaccacctaa acggacgtaa cggtatttga
     1081 tcaccgaatt gaccggcact ggcaatgacg ccgtgtaggt caggctgtcg cgcgcggtca
     1141 tgggatattt ggtttcgtta aaacgaatcc cagtcacttc atctacaaca gagatggcaa
     1201 gcgtatcgcc agcttgaagc ggctctggta atgtgacaat gaaagtagtt tgtgtggcag
     1261 cgtatggggt gttggttgca atggcaggcg caggtgtgcc agaatttgtg ttgccgctgg
     1321 aagggtcaaa cagagaaatg gtacaagctt gcccaacaag tgtcaaaacg atcgccgcgg
     1381 atatccaacg gcgcagtgga atgatcgaac ctttcatgtt cttctcctca aggcgcatca
     1441 gtgcgcttct ttccaaaccc tagcctagct caacgtcact ggctgcttcc tgaacttcgg
     1501 cgtttttgct tcgcccggcg taggcgagat aacttaagcc gatactgata aagtataaca
     1561 aaatcatcgg gaccatcacc aaccccatat tgaccgggtc tacggtagga gtgatggcag
     1621 ccgcaagcac ggcaataata acgattgcta atctccattg gtccgcaagg aatttcgggt
     1681 tgaccaagcc aatggcagta agtacataaa taagaagcgg aaattcaaag aatccgccaa
     1741 tccataacat cagcccggtg acgaatgaaa aatattctcg tgcagaccaa aattgcgaga
     1801 tggacgtgaa actccctaaa aagggcaagg cagtcggtaa taagacgtaa aaagtaaacg
     1861 ccataccaag tagaaaaaga agggtggcaa gcggaatgcc aaccagccct aacttctttt
     1921 cgcgaggctt caaaccgggt gcggcgaaca gccagaactc aaacgcaata taagggaagg
     1981 cgatcgcaat accaatggtc attgccacac gcatgaaaac gccgatctct tctgtcactt
     2041 caatggcttg cagcttttca agtccgccaa caggttcaga gagaaatgcc atggcaggtt
     2101 ctgccaccca gaatgccgcg atcactaaaa ttgataaggc aatgatcgag cgcaaaaggt
     2161 gcatgcgaaa ggcttcgatc tgttcccata actgatcgcg gacttctttt tccgtgacca
     2221 aatccgcaaa gacatccgca atgggacgtt cttcgggttc gctattaagc agcttgaaaa
     2281 aacgataacc tgcccggaaa gggaaggtta tgatggcata gacaaggcga aatggaaggg
     2341 tgataaccca ccaaacaccc ttaaagaact tacgcattat tcggagtttc gctttcgggg
     2401 ttgtttttct cttgcgggtt tggaaggata acattctcac cgccaggctt gatgggcggt
     2461 gtggcagaat atggctgagg ttgtggtcga gagtttgcgg gggcagaccg ggctgggtct
     2521 ggggtggagg cggcgctctt tatctcgcga ttgaccttat ccaaatcctc atttgcttcg
     2581 cggatcaagc gatttggcaa agtgcgcaag tcgcgcgagg tttgttgaaa aagtttccag
     2641 ccatcagatg tgatgatatc tcgtaaaaac tttccgatgg tcttgccggc tttttgcata
     2701 tctttcggac ccaaaataag caaagcaata atcacgacaa aaataagttc aggcattcca
     2761 atgccaagga tttccatgtt tatgattcca aattggtttc ttgaggtatt tcgtttttca
     2821 gaacctgata gatttcctgc tcatgctcaa ccaaggtacc aagcacacca tttacaaagc
     2881 gggcagtcga gtcagacccg aacaacttgg ctaactctac agcttcattg atcgcaacct
     2941 ttaccggagt ttggcgcgag attgcaaatt cccaacatgc catgcgcaaa atatttctat
     3001 cgatggcggc aatttgatcg agtggccact caggtgcata ctttgcgatc acgcggtcaa
     3061 ggttttctcg aatgggcaaa ataccaaaaa taatgctgcg ggcgaactct gctaaatctt
     3121 gcggcagagt ggtttcttct aaacgagttt tcaatacatc accgggcagg tgtgtggaaa
     3181 gatcagtttc gtacaatact tgcagggcga gcgcgcgcgc ccgggttcgg ggcttcattt
     3241 gattatgcct cgtccccttc aaaatcaata tcttcgatgt ggacattcac ttcgcccact
     3301 tccatgccaa ccatttcctg aacggaacgg gctacattcg cttgcacatt gcgcccaact
     3361 tcgcggatgt tgactccatc tttgaggatg aggaataggt caacaaagac cgtgttgtct
     3421 ttcgcttcaa tgcggacgcc gtcactggtg ccgcgccgaa agagccgatt cacgccgcca
     3481 ctgactggtg ccagtcggct aacgccgggg acttccaatg cggcgagact ggcgatagtt
     3541 gtcaacactt cgggagagac ggtagttttt ccttgcgtag attcagtcat tattactcct
     3601 gttttacatc attgccatgc cgccgtccac accaatgacc tgaccggtaa tgtacgcggc
     3661 gccgtcagag gcgagaaagg tagcagtaaa ggcaatttct tcgggttttc ctttgcgccc
     3721 aagcggcaca agtttgagcg cagcttcaag ggcttcgggt gtcatggcat cgagaatctc
     3781 agtatctaca aatcccggcg cgatagcgtt gacggtaatg ttacgagcag agacttcgcg
     3841 tgccaatgct ttggtaaatg cgatctgacc acctttggag gcggaatagt tcgtctgccc
     3901 ggggttgccc atttgcccag caaccgatgc catgttgatg atgcgcccgg tgcgtttgcg
     3961 catcatgtgc ttaacggcag cttttgagca attgaaggtg cttttgagat tggtgttgat
     4021 aacggcatcc cagtctgctt cgggcatcat cataatgagc tggtcgcgcg taatgccggc
     4081 attattgaca agaatatgca agtcgccaaa agtatcaatg gcaaacttaa ccaacgcctc
     4141 ggcttgctta aaatcggaga catcagcttg aaaggcggcg gcttttccac ccgcttcttg
     4201 aattttcttc accacttctt cagccgactc aggggatttg ttgtaattca ccaccaccgc
     4261 tgcgccgcga tcagcgaatt cgagagcgat ggcgcgtcca attccgcgtg aaccaccggt
     4321 aacgatagct actttattct ctaatgataa tttgaaggac ataactgggc tcctgtaaag
     4381 aaattatact tcatctggaa ttgagtaaaa attgaaatca aataggtgcc taaacccaaa
     4441 ttgtgcatac aaaccagctc cggcagagct ggcggttagg gcgactattt taccctcaca
     4501 ttgggctgct tctgaaagaa gccgtgaaag aatggctttc ccatagcctt gtcgatgaaa
     4561 ttcaggcaaa gttgccatca cataaatgcc ggggatacct tttgcttcta tgatggcacc
     4621 aattgaaact gcttcacctt ttttcggtcc ggcaaggaac aaacgagccg agctttcatt
     4681 agataaccaa tcttgtgcca tatcttcaaa ataagtcaac gcttcgcctt ctgtaaactg
     4741 gaatgccctg cgccgaatgg tacttgccat ttgcaaatct ttgatagatg ccgcacgcca
     4801 cacctgtatg gatttgtcta cttgtggaaa cgaggaagca tcttgttttg ttggtgcaat
     4861 catagctggt agtggcgggg catcgtacac acaaccgaat ttctctagaa ttgtcgcaat
     4921 gtttgcaggg cgcggctcag cattcatcca ccaataccaa gggacatttc tttccgtgaa
     4981 gaagtcgatg acttgtttta cttccgcttc tatttcagat gaagccgtct tgtctggaaa
     5041 gttggcacca agcgctagat ttaagagcgg cagttttaac cctgtagaag aggctctgac
     5101 tcccatgccc tgaaacgttt ttgccattgg gctgcgtgaa gaaagccaag cttggacgtt
     5161 taacgtatgt gcatctgcca tacggatgag gtctatttgt gaagacccaa ataactcaag
     5221 acattggtca ttggtaagag gcattttttc tccgtttgac gagatgatgc attgcctaga
     5281 tttataaaaa aataaccccg aactgatgac aacgatttca gagaaatgtc actcagatca
     5341 gggttaacat ataaatttaa gaaggtttag tttgttgttt ttagcccaaa tgctaaaccc
     5401 aacccgcctc ccaaggcgat cactgccatg gcaataactg catcttgcca gaagcggatg
     5461 gggaagagac gaatgagggt atctgaataa tagaagagcc aagagtcgcc ctcaaagaat
     5521 agtgagtgaa accaggcaaa gaaattccag aagatatcgg ggttgagcaa aatacctgcc
     5581 ccagcaatga aaccaagcgt gaccgccagc ccgatcatcc accaaccgcc gcggcgtaaa
     5641 ccagtgagat acaaaggcat ccatccgttg cgttgggcga ggatggcaag cagaataaga
     5701 acggcaatgg agagatacca aaccttcaaa gcgccttgca ctacattctt gacgtcatgc
     5761 atgtgactaa gctcagcttc gccataaata ggggtgccgt tatcgaattt taggtcgcca
     5821 agataagaaa tatcagcgct gttgacgaga tattccaccg aaggttttgc ccatttcaaa
     5881 cggtcttcct tcgtgaagcc gtattcatcg gcagggaagt agggcatatt gtattcaacc
     5941 gtgtaataaa gcggcgttag taaaatgcgc aatgccaacc cgatcagaac cagtggcgtg
     6001 gcaagagaga caagataaga gagaagagtt gggagtttca ttattgtaaa aattccaatc
     6061 cgccttcgag cacgaatttc acaaccatgt cgttgacgat ccattcaatg ggggcgagat
     6121 cgtcggtgaa gacagttgtg gtttcgtagc cgggtttcaa gtttgcataa gcagatgaca
     6181 tggtcgtcac aagtaatgga tttaagcttt tatcttctgc aatgcgcaac gtgtttgcga
     6241 ggaatgcatc tgctgttgta ggttgtttgg tggcgtagat catggtgttg agcgtgccgg
     6301 gaatatccat gatgtgaatg gatgggaaaa cttgagccat ggtggtggcg agtccatcga
     6361 taaggcggcg atctcccggt gtggatccaa cgttgagggt cagcactccg ttatcggcta
     6421 ggtgagaggc gcagatctca aagaattcaa gcgtcgtcat gtgtggaggg atgtagggcg
     6481 ggcggtacgc atccacggcg atgatgtcat atttttttgt ggagcggtca agattcaatc
     6541 gcccgtctcc gagaataaca tttaggtttg gcgaacccat gtgaaagtat tcgtagccaa
     6601 cttcagcgat ctttgggtcg agttcgtagc cgtcaatgtg caggtcttcg ccatagacaa
     6661 tcgtagcttg acgagccgct gtccctgctg caagcccaat gatcgccatt tctttgatgt
     6721 catctggcga acgattttca tagaagtatg gtcccaccat gaagagttcc catggaccgt
     6781 tgtagaaaag attctcagga tgatagatgg agtgaatgcc ctgaccgtcg ttgaggcgca
     6841 ataaagtgta tccgttcgac tcttgaactt gaatatagtt gtattcagat tcggtttcgt
     6901 acacctgccc tttattggtt tttaatgcag aacttgaaaa gataaatgca atcactgcca
     6961 gcaagatagg catccaaatt aatttgagca tttcgcgagt gctggcaaag cgagccaagc
     7021 caaccagtgc aaccactaaa agcgtcatac cgaaaatggc aaaggtgagg cgtgtgccaa
     7081 cggtgggaat aaaaacaaga gtgggcagga aggttccaat gaacgaaccc aacgtagaca
     7141 cagcatagat ctgcccggaa atttgaccag ctttgctggc atcatctact gagaggcgaa
     7201 tggcatatgg cgaaattgct ccaagcaagg tgatgggcac aataaagagg atgaggacaa
     7261 cgataaagga gcctgccatg atgccgacac tcaacgcctc aaacgcgctc gccgcggacc
     7321 tgagaacggg tacggcgaca taaggcacaa gccccactgt aaacgctccc cacgccaaaa
     7381 tgcgatacat tgctagtggg cttgggtctt tatctgccca tttgccgcca aggaagtatc
     7441 ccaacgtcag gtagatgagg atcaagccga tgatgcttgc ccaaaccaga ttgctggtac
     7501 caaacacatt accaatgagg cgccccgccg caagttcggc tgcaagcgtg gtcatgccag
     7561 agatgaaaac cgtaaagagt aggtatcgtt tcatgaaaat ccctataaga agatgcgcgg
     7621 attatagcgt acttcatgca gtgagaaata agaactccct tagcacctaa gggagttctg
     7681 gatgttactc aagcgaactg gtatcgtagt tatatttctt gaaatattcg atcattcttt
     7741 gacgaatttc gtcatcagat ggcaagagat tcgactcgca ttttgcggtg tgacttttta
     7801 cctcgccagt tttgaattca ttgtcttgca cattccatga ataccatttc tgacagacaa
     7861 tgaattgcag acttttgatc tgatagaacg gtggtttgat ctttttgggt gaacccgctg
     7921 tggtactgat aatctctcgc atggcgggcg gttctcctag caagagcgat gagccggtca
     7981 tccactcggg ctggtcaacg ttgatatagt ccagcaaggt ggcgggtatg tcaatgatct
     8041 gcgtgttgtt ttggatcgtg ccggcatggt cgttgtttgg gaagtggaaa atgattggga
     8101 tgcgctggtt gaccacatag cgataaccgt ggtctgtgta gatgacgatg atggtattgt
     8161 ctaacttgcc agactcttcc aaatacgtat atatctcgcg gacgtggtca tcaaaggtgc
     8221 gaatggcttt tttgtaaagt tctacatccc attctttttc tgactcagcg ctatcttcat
     8281 tttcttctac atgtttgtca tccgtcgaaa agagcggacc gtgtgtattc atgtaatgag
     8341 agaagacgaa gaccgggcgg tctgaggttt ccagcaagtt gatgatctgt tgtgtgcgtt
     8401 cttcatcggt catacgggca ttggggttgt tgacctgtag gaagggattt tccatttcct
     8461 tgatgaagaa aatatgaaac aaacggtcgc tggcgcgttc tgttacggtt tgaagaaaga
     8521 aggcagaagg agaatttccc aataacggtt gaatggcatc caagatgggg ttatcaacag
     8581 agacgttgtt tactaattca aagccatcag ccaagtttag ctttctcgca tctacataat
     8641 aagtagtacc gagttccacg gttttgtaac ccatgtgctt tagaatgccg ggcaaatgct
     8701 cgaaggaatc ttctttggag aggatatcgg gatagcgaaa gacctgaact tctgcaggca
     8761 ttttcccggt caatgcagag gtggtggagc cggtggtgct ggaaacgtta gggaaggcat
     8821 tttcagctac gagggaggtt tgggtcagtt cggcgatgaa gggtgtggta ttgccctcaa
     8881 acccgtatgc ggatagatag ctggcactca aaccatcact gcccaggatt aggatattgg
     8941 ggtgttctgc cgaggcttct gtcgcttcgg gagtaaagtc gctcatgtat ggagagcgcg
     9001 aattgtatat ggagagaatg accactgttg aaacgccgag caaccccaaa gaaaggaaag
     9061 aaacaccttt ccaaagcttt tgtctctcca caaagcggaa gacccaataa aagagcagca
     9121 caaaggcaac tgtatacaac aagcgccatg ctccagatgt ggagacaatc ccaaatttaa
     9181 aaacggtata ggtgaagtta tcgatcatca ccaaagcagt gacagatacc atcaaggcgg
     9241 ctggaacata cccgaaaaac ttccacttaa gagcgggcac agcaaaaaca accagcaacg
     9301 ccaataaaag cagaacgatc acaccgccgg tcgaaaacaa gacctcgatc ttgtcgagaa
     9361 gggaaagcgt ggatagagac gaagactgcg taacaaaaaa cagccattcc ataagggcgt
     9421 gaaagtatgc ggcgaggacg gtcagattga aaaatagaga taagggattt gtagattttg
     9481 ttttcataaa gtcggcatat tttacacccc tgcattaaaa aaggctttag gaattatgaa
     9541 aaatagacct tgccagaatt agtaccaacg tgctcgattt catttctatc atttgtattt
     9601 caacattgaa cccagcgctc acaaacggac tttttagcct ttctttcaag gcgtcgtcta
     9661 aggcttctgg gctttcgcca gtgaccttat acaaccactt taggaatcca tttgatggga
     9721 aggcggcagg tagaacgatc aatttccctt catttacaag aacccgcttt atttctgcca
     9781 gtgttttggg gtcgaagatg tattcacttg ggaaggtgga gataacggtt tcaaacgaag
     9841 cctttgcaaa tggcaagttt tgagctaaac cgcgcgtcag tttttgcttg gagccgagac
     9901 ggcgtttggc gatttgcccc atctgtgtag attcatctag ggcaacagga ttcagcccca
     9961 ggtccagtaa gaagcgctgc aagtgaccgg gaccatgacc caactcaaga aggcgagtcc
    10021 cttcaaggtg tggggaaatg ctacgaaccc agtcattcca cttcccaaac gaaacgaccc
    10081 acgccaccag gtcatatgtg aaagcgaagg gatggtaaag caggttaaag aaaaaacgca
    10141 tgaagtgacg catggcagaa ataaaacgag ggtttgacct gagtcaaacc ctcatctgtt
    10201 tcaataaaga ttaaataggg gtggcttcgc tgccgtcttt cttcggcttg cgaacggctt
    10261 cgatggcgcc cttgccgcgt tcttttactt cagaagcgag ctgttctgca cgagtgcgag
    10321 cttctttggt gagttcttcg gcgcgggtgc gagcttcctt ggcaagttct tcagcgcgag
    10381 cgcgggcttc tttggcggcg gcttctgcgc gggcataggc ttcttcagcg ctcacttggg
    10441 ctttatcgcg caattcaatc gatttgtcct tgatcaacgc acgggtttct tcgccagatt
    10501 gaggagcaaa gagcaaagca acaacagcgc cggtgagccc gccgacaaca aaacctacga
    10561 gaaaggctcc aaattcatca cggtcagaca tggtagttct cctttttagt cagatgatgt
    10621 catatactat ttaggtttta cacctaacaa ttctaacata cgctttaagc ctgccaaata
    10681 gccattaagt ttgatcacag gctcaacaaa attttctccc aaaaattctg cggtgccgcg
    10741 cagggtgttc acggtttcgc tggttgcctg caaaatgggg cgcacttcat tctgaagcag
    10801 gttgatgaga ctcgccaatt ggatgacaag cacgatcaac gcc
//
LOCUS       DAOQNJ010000018        10362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3034_length_10362_cov_9.116523, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000018 DAOQNJ010000000
VERSION     DAOQNJ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3034_length_10362_cov_9.116523"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..2230)
                     /locus_tag="PLG52_01120"
     CDS             complement(224..2230)
                     /locus_tag="PLG52_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNB40066.1"
                     /translation="MATKGKSVKKGAKAAPKKVAKKVVAKKKAAPKPSNTFQPTKIKL
                     LRPVPSDIDIAQAGKLKPITQIAKELGIRDDELELFGPYKAKIKLEILERLKNKPNGK
                     YIDVTAITPTPLGEGKTTTTVGLSQALGAHLGKKVITAIRQPSMGPTFGIKGGAAGGG
                     YSQIIPMEDFNLHLTGDIHAITASHNLVAAALDARVMHEKEQDDEKLFNALCPANKKG
                     QRKFSPTMLRRLEKLGIKKTNPDDLTPEERSRFARLDIDERTITWRRVVDINDRMLRE
                     VEVGRGKDEVGHAHMSGYDITVASEIMAVLALTTGIEDMRERFGKMVVATSKRGDAVT
                     VEDLGVAGAVTVLMKDAIKPNLMQTLEGTPATVHAGPFANIAHGQSSIIADKIALKLV
                     GKDGFVVTESGFGADIGMEKFFNIKCRYSGLIPHVVVMVATVRALKMHGGGPKVVAGK
                     PLAPEYTDENLELLKAGLVNLERHIQNAMKFGVKVIVAINSFATDTEAEVELIRKSAI
                     EFGAMDAIKCTHWADGGKGTKKLAEAVVKAAEQPSNFKFLYDPKLPIKEKIEIIAKEI
                     YRADGVDYSPEAEEQVERYTRLGFNDLPICMAKTHLSFTTDATKKGAPTGFRITVREV
                     RASVGAGFLYPILGDMRTMPGLPTRPSFYDVDLDMETGKVLGLF"
     gene            complement(2326..3126)
                     /locus_tag="PLG52_01125"
     CDS             complement(2326..3126)
                     /locus_tag="PLG52_01125"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNB40067.1"
                     /translation="MTNELVLLKLGGSLITDKDTPYTPRLDKLANLAKEIKTALDSTP
                     GLKLILGHGSGSFGHVAAKKHGTRDGVQTKEQWLGFTEVRLQAAELNRYVVTSLFEAG
                     LPALPFPPSASMVSDKRKVTHHNVETIRRALGVDLLPVVQGDVAFDETLGGTILSTED
                     VFTFLVDQFPTTRILLAGIEAGVWADFPARTKLVKQIQLSDYEAMRSGIKGSVSTDVT
                     GGMKAKVEEMLSLIQKKPGLTVQIFSAEEPGFLTRALQGENVGTLLTA"
     gene            3193..4038
                     /locus_tag="PLG52_01130"
     CDS             3193..4038
                     /locus_tag="PLG52_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40068.1"
                     /translation="MDIRTGVFVLALFAAVGAFLSFRGAIRNMQAARKISFYSLRKRY
                     NNTAWRLVFLAFVLIGVAFWFPSGGAQVMYQVFPPSVTPSLTPTITLTPTITLTPTIT
                     LTPTLTPTPLVSDTPTLTPTPFLPAAIEALFAGPVTPNPDSIFTAIQFSTEFDGVNAV
                     DPKTVFTLPIDTIYGGFDYNNTQPGVQWTALWFRNGELVCYETEPWREEWGTGGIGGY
                     TSCSSPLDGWQAGQYEVQIFMGYDWKVVGRFVVMDSLTPSVTVTGTPDLLQTGTPTIA
                     PTATP"
     gene            4061..4552
                     /locus_tag="PLG52_01135"
     CDS             4061..4552
                     /locus_tag="PLG52_01135"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNB40069.1"
                     /translation="MTEKAFQEYYPEHLSHCYGCGTMNELGHQIKSYWDGEESVCHFQ
                     PKSYHTAIPGYVYGGLLASLIDCHGTGTAAAAGYRAENRAMDTEPALRYLTASLHVDY
                     LKPTPMGPVLEIRARVKEVKGRKVVVEEWIQVNGVVTVKGEVVAVQVPEALVNELLEK
                     GAN"
     gene            4576..5094
                     /locus_tag="PLG52_01140"
     CDS             4576..5094
                     /locus_tag="PLG52_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40070.1"
                     /translation="MKKVWIGILLGVVLAGCSTNVPPEMRARMMDTENVPSEWIIFNE
                     SKGEDWGGQLYTVAFAYGNESESPALEQQLIVYSDESAAITGYDEFDAYIFVEEWTVN
                     PEITFSPSTTTDKFAAKCTDREIDHVMTKICFVLQQHANYVSALGVHMGGPITFDVLN
                     SVLKSIDTKLNQ"
     gene            complement(5138..5563)
                     /locus_tag="PLG52_01145"
     CDS             complement(5138..5563)
                     /locus_tag="PLG52_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HNB40071.1"
                     /translation="MSITLEQKRLVQETWEKVVPIAETAAELFYGRLFEIDPELKPLF
                     KNADMKEQKMKLLQMLGMAVKGLDNLDQLVPAVENLGRRHVGYGVKDSHYDTVGDALL
                     WTLEKGLGEDFTPEVMEAWTETYVTLATVMKNATETVAA"
     gene            complement(5714..6103)
                     /locus_tag="PLG52_01150"
     CDS             complement(5714..6103)
                     /locus_tag="PLG52_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB40072.1"
                     /translation="MKNEFVLSETFNANAAAIYNAWLSTQGHTLMTGSPAKVNGHAKG
                     DFTAWDGYIWGTFLDLEPNKRIVQLWRTGEFPDDAEDSRVEILLEETNGKTKLTLRHT
                     HMPEDQIESYKTGWLDFYFKPMKEYFG"
     gene            complement(6141..7067)
                     /locus_tag="PLG52_01155"
     CDS             complement(6141..7067)
                     /locus_tag="PLG52_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNB40073.1"
                     /translation="MKHPHIQLSVEVSRALTMGAPIVALESTVITHGLPQPQNLELAR
                     NMEQQVRDAKATPATVAVLDGKIRLGISDEELVRLSESQSTLKVSHRDFATAIVKKVN
                     GGTTVAGTMLAANMSGIKVFATGGIGGVHKESAFDISTDLKALAETPMVVVCAGAKAI
                     LDLPATMEYLETMGVPVIGYKTDEFPAFYSRESGLNVSARLDSPEEIAEFAKAHWSLG
                     LRSAILVTNPVPEADAISKSEMEPMIAQASAEAVEKGIHGQALTPFLLGRISELTKGK
                     SLKTNLALLLNNARLAGEIANQMYTRKKEWAI"
     gene            complement(7070..8659)
                     /locus_tag="PLG52_01160"
     CDS             complement(7070..8659)
                     /locus_tag="PLG52_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40074.1"
                     /translation="MNRFRRRRDEMIPRIFALTDEAVEYRSQIRNYARIVVWAVLSGF
                     TLILFFFFAETTIQRLTVLGLSLLIYLPLFSAAQTVARHKAANYLADIYEIEDETIAD
                     SFLEEVIFGEGTEKITINEGKISERDEESPLIMIGGPGYIQVNLDSVALLERVDGTPE
                     IIHPRGKPWKLGSFERIREIGKHDEPGKREYAIINLRDQFVRGLSVRSRTKDGIPLEA
                     QDIKVLFSILRKPKEEAPENDPYHFDETAVHSLVYNQTIISPSPAKTAGVSFPWDTTV
                     IPLITSEMENLITSRNLSEILASISHKEIDDLNEGEATNKQIRLEMTGEQTVPTNSNS
                     PSAPNFESRSKITAQFFSPQFKAKAAKLGVSIHWIDIGTWQLPSEMILEELKSGWKMM
                     RENVRRRNDVNRLAKRQEMKELRELVNHVIVSSFTKANNANDNRKLSDDEFSKLSKII
                     EENPNIAFSPALQQKMAQDVTLKKDAALEILKAFRKEFIAARELIQKENRSSIDKQAE
                     IARIDKALRDIDYHVFHYIKG"
     gene            complement(8656..10074)
                     /locus_tag="PLG52_01165"
     CDS             complement(8656..10074)
                     /locus_tag="PLG52_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40075.1"
                     /translation="MKSAFSGIGSFLQTPFGVIAAGTVVFHGILFAVGFNYTVVTLVI
                     AVVVLGVNLFAANYIADKLVYFYSQFVLPVQTPKDRQEIYTRVSGFDGSRGPILFVKN
                     GRVIKHEGETEKKGPGVIVLDTASAIVTQTETEIIGAAGPGIHFTKGREKITRGEGVD
                     LRPQWQFIGPLASDQPFLNPPSPDSKKYNELQARRQQTAGQTRDGFEISPTISIKFRI
                     KQPEEKTPSESGVVSHYGYNATAVLHAVTREVIELGTSDNKRTRMEWYRLPAHLVVNL
                     WREYVRKFKLEDLFKAEGVSGLQTIEEMINRRVKRPEVVAMDDTGIPTGEALPSLEYQ
                     QLIERGLEVIDVRIHNVLFDPAIEEQTIKNWNNEWTKIAKREEDLLNEREKLIETAAH
                     NEAVKRFARLASLKFENPIAMPADAYTTLQALIEPVKETLVIESRANSQMETDIKKLD
                     EIWKWLLVSKLDKSMHRKEDES"
BASE COUNT         2472 a         2460 c         2544 g         2886 t
ORIGIN      
        1 tcgggttgag acggtcaatt tgacggtaac aagccgcggc aaggtcagaa gcgctgattt
       61 ccttactttt gatcagttct ctttgttcag ctaaagtcag ggaagcggga ttcataaggg
      121 tgaattatat tcaatttata cttattttca atatagccta tcggtactga aaaaacaggt
      181 cacgcctaac gcgtgacctg catttttgta ggtagagatt gttctagaac aacccgagga
      241 ccttgcctgt ttccatatcc aagtccacat cgtagaaaga tgggcgagtg ggcaaaccgg
      301 gcatggtgcg catgtcgccg aggatggggt acaagaagcc tgcaccaacg ctggcacgca
      361 cttcgcggac ggtgatacgg aagcccgtag gcgcaccttt cttagtggca tcagtggtaa
      421 aggaaagatg ggtctttgcc atgcaaatcg gaaggtcgtt gaacccgagg cgcgtatagc
      481 gttcgacctg ttcttcggct tcaggcgagt aatccacacc atcggcacga tagatttcct
      541 tggcgatgat ctcgatcttt tccttgatag gaagcttcgg atcatacagg aacttgaagt
      601 tgctcggctg ttctgcagct ttgaccaccg cctcagccaa tttcttagtg cccttaccgc
      661 catcagccca gtgcgtacat ttgatggcat ccatggcgcc gaactcaatg gcagatttgc
      721 ggatgagttc aacctcagcc tctgtatctg tcgcaaatga gttgatagca acaataactt
      781 tgacaccgaa tttcattgca ttctgaatat ggcgttcaag gttaacgaga cccgccttga
      841 gcaattccag attttcgtcg gtgtattcag gggcgagcgg tttacccgcc accacttttg
      901 gaccaccacc atgcatcttc aatgcgcgga cggttgcaac catcaccact acatgcggga
      961 tcaagccaga ataacggcat ttgatattga agaacttttc cataccaata tcggcaccga
     1021 aaccagattc agtaaccacg aagccatctt tgccgactaa cttgagcgca attttgtcag
     1081 cgatgatgga agattgtccg tgagcgatat tagcgaaagg tccagcgtga accgtggcgg
     1141 gagtcccttc gagagtttgc atcaggtttg gcttgatggc atccttcatc aaaacggtga
     1201 cagcgccagc cacaccaaga tcttccacag tcacagcgtc gccgcgtttg ctggtggcaa
     1261 ccaccatctt gccgaagcgt tcgcgcatgt cttcaattcc agtggtcaat gcgaggacag
     1321 ccatgatttc tgaggcaacg gtgatgtcgt agccagacat atgtgcatga cctacctcgt
     1381 ctttaccacg acctacttcc acttcacgca acatgcggtc attgatatct actacgcggc
     1441 gccaagtaat ggtacgttcg tcgatgtcga ggcgagcgaa gcgggagcgt tcttcagggg
     1501 tgagatcatc tggatttgtt ttcttaatgc caagtttttc caaacggcgt aacatagtgg
     1561 gtgagaattt gcgctgtccc tttttattag cagggcagag cgcgttgaag agcttctcat
     1621 catcttgttc tttttcatgc atgacacgcg catcaagagc ggcggcaacc aagttatgtg
     1681 aggcggtaat ggcatgaata tcgccggtga gatggagatt gaaatcttcc atcggaataa
     1741 tctgggaata accaccgccc gctgcgccgc ctttgatacc aaaggtagga cccattgaag
     1801 gttggcggat ggctgtaatg acctttttac cgaggtgcgc accgagcgcc tgagaaagac
     1861 caaccgtggt tgtggtcttg ccttcaccaa gcggagttgg cgtgatggca gttacatcga
     1921 tgtactttcc attgggttta ttcttaagac gttcaaggat ttcaagctta atcttggctt
     1981 tgtaaggtcc gaacaattca agttcgtcat cgcgaatacc aagctctttg gcaatttggg
     2041 tgatcggctt aagctttcca gcttgtgcaa tatcaatatc cgaaggcaca ggtcgaagga
     2101 gctttatctt agtcggttgg aatgtgttgc tgggcttggg agcagctttc ttctttgcta
     2161 cgaccttttt tgcaactttc ttaggcgcag cttttgcacc cttcttaacg gactttcctt
     2221 tggttgccat agagtctcct tgaaaattat atataattta ttctatcccc aattatcaga
     2281 caaatttaaa aaattgcaag agcaaaccgt catttttttg tcatttcatg cagttagcaa
     2341 cgttcccaca ttttcacctt gcaaggcacg tgtcaagaag cctggctctt ctgccgagaa
     2401 aatttgtaca gtcaaacccg gcttcttttg gataagggac agcatttctt ccactttggc
     2461 tttcatccca ccagtgacat cggtgctcac ggaacccttg atgccagacc gcatcgcttc
     2521 ataatccgac agttgaatct gctttacgag ctttgtcctc gctgggaaat ctgcccacac
     2581 tccggcttca atcccagcca gcagaattcg agtggtaggg aattggtcca cgaggaaggt
     2641 gaaaacatct tcggtggaaa gaatggtgcc acctaatgtt tcatcaaaag cgacatctcc
     2701 ctgcaccaca ggtagcaaat ccacgcccaa ggcgcggcgg atggtctcta cattgtgatg
     2761 ggtcactttc cgtttgtctg aaaccatcga agcagatggc gggaaaggca acgcaggtaa
     2821 cccagcctca aagagagagg tgacaacata gcgatttaat tccgcagctt gcagtcgaac
     2881 ttcagtgaat ccgagccatt gttccttcgt ttggacgcca tcgcgagtgc catgcttctt
     2941 tgcggcaacg tgcccgaacg agccagaacc atgcccgaga atcagtttca agcccggggt
     3001 tgagtccaga gccgttttga tttccttggc aaggtttgcc agtttatcca gccttggggt
     3061 gtagggagtg tctttatcgg taatgagcga tccgcccagt ttgaggagaa cgagttcgtt
     3121 ggtcatagtg cttgttttaa cacagatttt tgaaattgtc gcgcactttg ctttactggg
     3181 tataattttg gcatggatat tcgtactggt gtcttcgtcc tcgccctgtt tgcggctgtg
     3241 ggggcgtttc tttctttccg tggagcgatc cgtaatatgc aggcggctcg caagatttct
     3301 ttttatagct tacgtaagcg ttacaacaat accgcttggc gtttggtttt tcttgctttt
     3361 gttttgattg gcgttgcttt ttggttccca tctggtgggg cgcaagtgat gtatcaggtc
     3421 tttccgcctt ctgtcacccc atcgttgacg ccgacgatta cactgacgcc gaccattacg
     3481 ttaacgccga ccatcacctt aacgccaaca ctaacgccca cgccattggt ctctgataca
     3541 ccaacattga ctccaactcc gttcttgccg gctgcgattg aagcgctttt tgctggaccc
     3601 gtcacgccga accctgattc gatatttacc gccattcaat tctctacaga gtttgacggc
     3661 gtaaatgcag tggacccaaa gactgttttt acacttccta ttgacacgat ttatggcggt
     3721 ttcgattaca acaacacgca acccggcgtg cagtggaccg cactctggtt tcgcaatggc
     3781 gaattggttt gttatgagac cgaaccttgg cgtgaagaat ggggcacggg cggtattggt
     3841 gggtatacct cttgttcttc acctttggac ggttggcagg cagggcaata tgaagtgcag
     3901 atcttcatgg gatacgattg gaaagtcgtt gggcgttttg tggtgatgga tagcctgacc
     3961 ccttctgtga ccgtaacagg gacgccagat ttacttcaaa caggaacccc aaccatagcg
     4021 ccaacggcaa caccgtaaat ctataacaac aggaatacac atgacagaaa aagcctttca
     4081 agaatactat cctgaacatt tatcgcattg ctatggttgc ggcacgatga atgaattggg
     4141 acatcagatc aaaagctatt gggacgggga agagtcggtg tgtcactttc agcccaagtc
     4201 ttaccatacc gcaattcctg gttatgtata tggtgggctg ctcgcttcac tcattgactg
     4261 tcacggtacc ggaaccgctg cagcggctgg ataccgtgct gagaaccgtg ccatggatac
     4321 agagcctgcg cttcgctatc tcaccgcttc tttgcacgtg gactatctca agccaacgcc
     4381 gatgggacca gtgcttgaaa tccgcgcgag ggtgaaagaa gttaaggggc gtaaggtagt
     4441 ggtcgaagag tggattcagg ttaatggagt ggtcaccgtc aaaggagaag tggtggcggt
     4501 acaagttcca gaagcattgg tgaatgaatt gcttgaaaaa ggtgcaaatt aaattcaaag
     4561 agtagagaat aaaaaatgaa aaaagtatgg attggtattt tacttggggt ggtcttggct
     4621 ggctgttcaa cgaacgtccc gcctgaaatg cgtgcaagga tgatggatac agaaaatgtc
     4681 ccttccgagt ggatcatttt caatgaatcc aaaggtgaag attggggcgg tcagttgtat
     4741 acagttgcat tcgcgtatgg gaatgaatca gaatcgccgg cattggaaca gcaattgatc
     4801 gtgtattcag atgagtctgc tgcgataact gggtatgatg aatttgatgc gtatatcttc
     4861 gtggaagagt ggacggtcaa ccctgaaatc acgttttccc cgagtacaac gacagacaaa
     4921 tttgcggcaa agtgtacaga ccgtgaaatt gaccatgtga tgaccaaaat ctgttttgtg
     4981 ttacagcaac atgcgaacta tgtctctgct ttgggcgttc acatgggcgg acccatcacc
     5041 tttgacgtgt tgaactcggt cttaaaatct attgatacaa aattgaacca ataaaaagaa
     5101 ggtctcgcta aaacgagacc ttcttttttt agaaccacta ggcggctaca gtttcagtcg
     5161 cattcttcat tacagttgct aaggtgacgt aggtctctgt ccaagcttcc atgacttcgg
     5221 gggtaaagtc ttcgccgagc cctttttcca gcgtccacag cagggcgtcc cccacggtat
     5281 cgtagtgaga gtcttttacg ccatagccaa catggcggcg accaagattt tccaccgcag
     5341 gcactaactg atcgaggtta tccaaacctt ttacagccat gccgagcatt tgcaataact
     5401 tcatcttttg ttctttcata tctgcattct tgaacaaagg cttaagttca gggtcgatct
     5461 caaaaaggcg accgtagaat agttcagcag cagtttctgc gatcggtacg actttttccc
     5521 aggtttcttg cacaagtctc ttttgttcta atgtgatgct catttctaaa atactcctct
     5581 aaagttaatt gattaatacg agcgcgattt tataagcatc gcttggaatt gaccatgtga
     5641 gtttggtact aaaactcaga taggacatta ctcctataaa aaagagctgc attttatgca
     5701 gctctttttt attttatcca aaatattctt tcattggctt gaaataaaaa tcaagccagc
     5761 ctgttttata gctctctatt tgatcttcag gcatatgtgt atggcgtagt gtgagttttg
     5821 ttttgccatt agtttcttcc agcaagattt ctacacgaga gtcttcggca tcgtcaggga
     5881 attctccggt gcgccaaagt tgcacgatgc gtttgttggg ttccagatca agaaaggtgc
     5941 cccaaatata cccatcccat gcggtgaaat cacccttggc gtgcccgttg acctttgccg
     6001 ggcttccggt catcaaggta tgtccctgtg tggacaacca ggcgttgtat atggctgcgg
     6061 cgttggcatt gaaggtttca gaaagaacaa actcattttt catcctgcct ccggcaatca
     6121 aacgaatcta cgctatctat ctaaatcgcc cattcctttt ttcgcgtgta catctggttt
     6181 gcaatttcac ctgctaaacg tgcattgttc aataacaatg ccagattggt cttaagagat
     6241 ttaccctttg tcaattcgct aatacgaccc agcaagaaag gagtcagtgc ctgcccgtgg
     6301 atgcctttct ctacagcttc agcagatgcc tgagcgatca tcggctccat ttcagacttt
     6361 gagatggcat ctgcttcggg aaccggattc gtcacgagaa tggcacttcg taaccccagg
     6421 ctccagtggg ctttggcaaa ctcggcaatc tcttccggag aatccaaccg cgcgctgacg
     6481 ttgagtccgc tttcgcggga gtagaaagca gggaattcgt cggtcttgta cccgatgacc
     6541 ggtacaccca tggtttcgag atattccatg gtggcgggta ggtctaaaat agctttcgct
     6601 ccagcacaga ctactaccat gggggtctca gccaatgctt ttaaatcggt ggagatatcg
     6661 aaagcagatt ctttatggac gcccccaata ccgcctgtag caaaaacttt gataccgctc
     6721 atgttggcgg cgagcattgt cccggcgacg gtggtaccgc cgttcacttt tttgacaatg
     6781 gcagtggcaa aatcgcggtg actcactttc agcgtggatt gactttcaga aagccgcacc
     6841 agttcctcgt ctgagattcc gaggcggatc tttccatcca atacagccac agtggctggc
     6901 gtcgcctttg catcgcggac ttgctgctcc atgttgcggg cgagttccaa attttgcggt
     6961 tggggtaaac cgtgcgtgat cactgtcgat tcgagagcga caataggcgc gcccatcgtg
     7021 agcgcgcgag aaacttcaac agaaagttgg atgtgggggt gcttcattct taacctttga
     7081 tgtaatggaa tacgtgatag tcgatgtcgc gcagggcttt atcgatcctt gcgatctcgg
     7141 cttgtttgtc tatggaagag cggttctctt tttggatgag ttcgcgggct gcgataaatt
     7201 ctttgcggaa ggctttgaga atttcgagcg cggcatcttt tttgagggtg acatcttgtg
     7261 ccatcttttg ctgtaaggca gggctgaagg cgatgttcgg attttcctcg atgatcttgg
     7321 ataactttga aaattcatcg tctgaaagtt tgcgattgtc gttggcgttg tttgctttgg
     7381 tgaaactgga aacaatgaca tgatttacca gttcgcgcaa ctctttcatt tcctgccgtt
     7441 tcgcaagtcg gttgacatcg ttgcgccggc ggacattttc acgcatcatc ttccaaccgc
     7501 tttttaattc ttcgagaatc atttcagaag gaagctgcca tgtgccaatg tcgatccaat
     7561 gaatgctgac gccgagtttg gcggctttgg ctttgaactg cggactgaaa aattgcgctg
     7621 tgatttttga acgtgactca aagtttggtg cactgggaga attgctgttg gttggaaccg
     7681 tttgctcgcc cgtcatttcg aggcgaattt gtttgttggt cgcttcacct tcgttgaggt
     7741 catcaatttc tttgtggctg atactggcaa gaatttcgct caagttgcgc gaagtgatga
     7801 ggttttccat ttcgcttgtg atgagcggaa taacggttgt atcccatggg aaagaaacgc
     7861 cagcggtttt tgcaggcgaa ggtgaaatga tagtttggtt atacaccaag gaatggaccg
     7921 cggtttcatc gaaatgatac gggtcatttt caggcgcttc ttctttgggc ttgcgcaaaa
     7981 tgctgaaaag cactttgata tcttgcgctt caagcggaat accgtctttg gtgcgcgagc
     8041 ggacggaaag tccgcgcacg aattggtcgc gcaggttgat gatggcatat tctcgtttgc
     8101 cgggctcatc gtgtttaccg atctcacgga tgcgctcaaa actgcccagc ttccacggct
     8161 tgccgcgtgg gtgaatgatc tcaggagtgc catctacgcg ttccagcagc gccacactat
     8221 ccaagttgac ctgaatatag ccgggtccgc cgatcatgat gaggggcgac tcttcatctc
     8281 tttctgagat tttgccctcg ttgatcgtga ttttctctgt cccttcgccg aagatcactt
     8341 cttcaagaaa actatcggcg atcgtttcat cttctatttc ataaatatct gccaaataat
     8401 tggcggcttt atgtcttgca acggtttgcg ctgcactaaa caaaggtagg tagatcaaga
     8461 gacttaagcc caacaccgtc agtcgttgta tcgtagtttc ggcaaagaaa aagaacaaga
     8521 ttagtgtgaa tccgcttaat actgcccaca ccacaatgcg ggcatagttg cgaatctggc
     8581 ttcgatactc gacagcctca tcggtgagag caaagatgcg cggaatcatt tcatcgcgtc
     8641 gacggcggaa tcgattcatg attcgtcctc tttgcgatgc atggatttat ccaacttgct
     8701 taccaacaac cacttccaaa tttcatctaa tttcttgatg tcagtttcca tctgactgtt
     8761 ggcgcggctt tcaataacca aggtctcttt tactggttcg atcagagctt gtaatgttgt
     8821 gtaggcatca gccggcatgg caatcggatt ctcgaacttt agcgaagcga gccgcgcaaa
     8881 acgtttgaca gcttcattat gagccgctgt ctcgatcaac ttctcgcgtt cattcaataa
     8941 atcttcttca cgcttggcga tcttggtcca ctcgttgttc caattcttga tggtctgttc
     9001 ttcaatggca gggtcgaaca acacattgtg aatccgcaca tcgatcacct caagaccgcg
     9061 ttcgatgagt tgctgatatt cgaggctggg caatgcttca ccagttggaa ttccggtatc
     9121 gtccatggcg actacttcgg gtcttttcac ccgacgattg atcatttctt cgatggtttg
     9181 taacccgctg acgccttcag ctttgaatag atcttccagc ttgaacttgc gcacatactc
     9241 gcgccacaaa ttcaccacaa ggtgagccgg aaggcgatac cattccatgc gagtacgttt
     9301 gttatccgaa gttcccaatt caatgacttc gcgtgtcacc gcatggagga cggcggtggc
     9361 gttatagcca taatgcgaaa cgacaccgct ttcagaaggg gtcttttctt ctggctgctt
     9421 aatacggaac ttgatactga tagtggggga tatttcaaac ccatcgcgcg tttgcccagc
     9481 ggtttgttga cgccgagcct gcaactcgtt gtattttttc gaatcaggcg aaggcgggtt
     9541 gagaaacggc tggtcactcg ccaacggacc aataaactgc cattgcgggc gcaaatctac
     9601 gccttccccg cgggtaatct tttcacgtcc cttcgtgaag tggatgccag gacctgccgc
     9661 gccgatgatt tctgtttcgg tttgggtaac aatggcgctg gcggtatcga gcacgatcac
     9721 acccggacct tttttctcgg tctcgccttc gtgcttgatc acccgtccat tcttgacgaa
     9781 gaggatcggt ccacggctgc cgtcaaatcc gctgacacgg gtgtagattt cctgccggtc
     9841 ttttggtgtt tgaacgggga gcacaaattg agaatagaaa tagaccagtt tatcggcgat
     9901 atagttcgcg gcaaaaaggt ttacgcccaa gaccacaacc gcgatcacca gggtaacaac
     9961 ggtgtagtta aaaccgaccg caaacaaaat gccatgaaag acgaccgtcc ctgctgctat
    10021 aaccccaaaa ggcgtctgta aaaatgaacc gattccgcta aaggcgctct tcattgtcgc
    10081 atgattctaa atcaaaattt gccaatctgg cgggctgaaa tataacaggt tgctttgaat
    10141 tttcgaatcc cccgaatggt cccccttaag tggcatggca atatgatata ttttcggcgt
    10201 tttgcacact gggaaaaacg tccagaacct aaaataagtg taaaccgtga aaagtgcttg
    10261 taaacgctct acttccgtat tgcgggtgta aaattccctc gttcttgtgt aaatagaact
    10321 taagttctat aattcattca gttgcattta tgtaacgcca ag
//
LOCUS       DAOQNJ010000019        10294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3057_length_10294_cov_10.033206, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000019 DAOQNJ010000000
VERSION     DAOQNJ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3057_length_10294_cov_10.033206"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLG52_01170"
     CDS             complement(<1..706)
                     /locus_tag="PLG52_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNB40076.1"
                     /translation="MIRIGDFSKLSRVSIKTLRYYDEMGLLKPVEVDRFTGYRYYDFH
                     QLPRLYRILALKELGFSLEEIGRFLEENLSTEELRGMLKLRQAEIRQRVEEEAARLEQ
                     VERWLRQTQQENFMSKYDVVIKKIDSMKAATVRGVVANPPAQRSLWDELLGFIYQNNI
                     RMNGTPMAIYHDADAREGDWDIEVAMPIAGDPTPVGRVKVYDLPAVETMACVVHAGSF
                     ATIGEAYDALAKWIDVN"
     gene            818..1369
                     /locus_tag="PLG52_01175"
     CDS             818..1369
                     /locus_tag="PLG52_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40077.1"
                     /translation="MDSKPTFDNFPLLSTPRLLLRDLRPTDLDDLYEYASDPEIDRYT
                     PWDRYQSLDDAREDLGGYIARYEQGHFRAWGIEHRADKKLIGITNFGFLSKEDRRAEM
                     GYTIARKYWGQGFATEAAQALIRYGFEKLDLARIEAVVLPENKASSKVMLKVGMQYEG
                     LLRSYQVWKGKPSDLEIYAIVKA"
     gene            1371..1892
                     /locus_tag="PLG52_01180"
     CDS             1371..1892
                     /locus_tag="PLG52_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40078.1"
                     /translation="MAIILETERLILRHQVLSDLDDLWALYQDPEITKYIPDAPRSRE
                     EAQKELEWHMHGHPRRPELGLWATIHKETGKFIGRCGLLPWEIDGQNEVEVAYTIAEA
                     YQGQGLGSEAALAILNYGFSKLKLSRLICLIDPENIPSQKVAEKMGMRFEKESRDEYG
                     MFWIYSIKKEAGN"
     gene            complement(1916..3892)
                     /locus_tag="PLG52_01185"
     CDS             complement(1916..3892)
                     /locus_tag="PLG52_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNB40079.1"
                     /translation="MNILVTLCLGGLGVLFLVLLTGIRFIPNNRIGIVEKRFGGKSLK
                     SGLIAINGETGFQPQVLRGGLHYRMPIQYAIHIAPLVTITQGKIGYVFARDGAPLEPA
                     QVLASNVTAHDFQDVTTFLKNGGQKGPQRLILREGTYAFNLMQFVVITEERVYSLPLS
                     RDDTAVIVRMAEVIKERDGFRPVIIKDGDDKIGIVTVHDGPSLPQGEIIAPVVGDNPE
                     KADEYHNKFQDADRFLKANGMRGRQLQVLVEGTYYINRLFATVEMIHKTIIDVGNVGV
                     VVSYTGEQGVDLSGSEYRHGELVTRGQRGVWSEALLPGKYPFNTYAGKVIPVPTTNFI
                     LKWIKNEVGSHNFDENLTEVSLITKDAFEPSLPLSVVVHIDYKKAPLVIQRFGDIKRL
                     VEQTLDPMVAAYFKNVAQTRTLIQLLQERSAIQQQSSLEMKEKFEHYNLELEEVLIGT
                     PSSPAGDKQIETILTQLRQRQIAEEQVETYSQQEKAAVKERELREAESRAAMQTKMTE
                     AELSISIQSNQGKAEYERSIQQAQQIRALAEAEAEKVARIGIAQALATEEQVRAYGGP
                     QFQVTQQVLNRFAEAIQQSGVDVVPRVVVGGSNGENGGNQSANIMEGLLTMLLSERFG
                     VSGEASTTQRSPDVEAMREKILKDMQKKDDTKKA"
     gene            complement(3946..4665)
                     /locus_tag="PLG52_01190"
     CDS             complement(3946..4665)
                     /locus_tag="PLG52_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40080.1"
                     /translation="MQNQSKSYNQFFIMFALGVLSGLVLITLATWADLEAAYYGFSRR
                     ASTPLSGFSCPVLMTATETNTVSLTLKNTTDGKISPTILVETSSPVAAFETTENLELA
                     AGEQTTAEWTVGPENLDLKRFIFAKALVYSSYPFPDRETTCGIFVLNAPGNGTIIVWG
                     FVILSLLGMGIGLYGVHQFQGPVKRGADVFKQFLFLTIVVLTGFLSIYISSWLLGILV
                     LVVSILFVIISTGIVLGRHHI"
     gene            4846..6645
                     /gene="lepA"
                     /locus_tag="PLG52_01195"
     CDS             4846..6645
                     /gene="lepA"
                     /locus_tag="PLG52_01195"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNB40081.1"
                     /translation="MTDHIRNFCIIAHVDHGKSTLADRLLQLTGTVSERDMTDQALDS
                     MDLEREKGVTIKASAVRMFYTAKNGQKYELNLIDTPGHVDFGYEVSRALKACEGAILV
                     VDATQGIEAQTLANLYQALDADLTLVPVINKIDLPSARPDEVAEDVGSLLGIDPDSVL
                     RVSAKEGINVDQILEAIVERVPAPKDADAEPLRALVFDAHYDSYKGVIAYIRVFEGKI
                     KTNDVLRMFATSNDLRPVEIGIFAPGMKPVDVIGSGEVGYVATGFKTVHECRVGDTLT
                     LAASPAAEPLPGYFLPKPMVFAGIYPVEADDYSELREALEKLQLNDASLTYQPETSQA
                     LGFGFRAGFLGLFHMEIIQERIEREYLLDVLFTAPSVEYEVLTIGDEVVTVDSPAELP
                     DPSKIVEVREPWMNIEIITPTDYYGAIMDLVTKRRGIFKQQEHPAPHRVQLDFEIPLS
                     EIIVDFFDHLKSRTKGYASLDYQFLEYRADKLQKLEILVNGDTVDALAAIVHEKDAYH
                     KGQRLITKLKDLIPRQLYDVAVQAASGGRIISRATVKATRKDVLAKCYGGDISRKKKL
                     LEKQKRGKKRLKMVGNVEIPQEAFMAVLKLEDE"
     gene            6706..7719
                     /locus_tag="PLG52_01200"
     CDS             6706..7719
                     /locus_tag="PLG52_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB40082.1"
                     /translation="MSETQNNLKRILPGVLVSIALIAAILYFVDIKTTLNALRNANYG
                     LIGIAAIFSFIWLAVRAVVWRTLLRNKPTYKDVFFTAGEGYLLNNFLPFRLGEIGRAF
                     LLSRKSGGMTFAEIIPTIVIERAVDLIISAVIFLAALPYVVNAESSPTLGYILGGVMV
                     FGLVFMYILARNNQWALDLFHKLSGRFPALQKVGGSFLESFFQGLGVLTDGWLFLRFL
                     FWMILNWAIALFAYYLMVLAFFPEAKPIWAIFGLGMAAFGGAVPSAPGAVGTFEGAVV
                     FALTRFTSDESTAFAAALTMRIYNYLNSTVVGMLGLSSEGQTLSGVYKQLINFRNRKT
                     DEE"
     gene            7709..8737
                     /locus_tag="PLG52_01205"
     CDS             7709..8737
                     /locus_tag="PLG52_01205"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB40083.1"
                     /translation="MKNKQYLVTGGAGFIGSNYVHRLLKRGEKVTIYDNFSRGGAPRN
                     LEWLKAEFGEKAFEVVVGDVRDAARIAEAAKTSDVIVHLAGQVAVTTSVTHPRDDFEA
                     NALGTFNVVEAARASGRQPIVIYASTNKVYGGMEEVELFEEPTRWRYKDLIHGCPETQ
                     PLDFHSPYGCSKGTGDQYVRDYARIYDLPTVVFRQSCIYGPRQFGIEDQGWVAWFIIA
                     AVMGRNITIYGDGKQVRDILHVHDLIQAYDLAVEKIDVARGQVYNLGGGPSNVMSIWS
                     EFGPRLEALLGKKIEVGRGDWRPGDQRVFYADVSKAKNELGWEPKISVEQGVNMLFEW
                     VQANKNLF"
     gene            8848..9678
                     /locus_tag="PLG52_01210"
     CDS             8848..9678
                     /locus_tag="PLG52_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNB40084.1"
                     /translation="MPHQLLSLVDVAMATLPNVLISLAIFAVSYYAGVWLGRLLKGVL
                     TRKNAEPGVSHLLSQILKWTIISLGFITALQRFFDVTAFLTGLGIIGFTIGFALQNIM
                     QNFVSGIILLIQQPFKVSDEVSLMGNEGFVQKIGLRTTEIRLRNGLLVFLPNADVLSQ
                     PIINFSRANGFRVELPVSIPYEADTEKARSIILDQVRTVPGCLETPVAQVFFHTFNAS
                     SVDLRVQFWVDNRTTDHHAAKDMVLVRIKQAFEENGIDVPYPVQVMKNPTKVTKRKTK
                     "
     gene            9675..>10294
                     /locus_tag="PLG52_01215"
     CDS             9675..>10294
                     /locus_tag="PLG52_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB40085.1"
                     /translation="MKILTVLTYYRPHTSGLTIYAERLAHAFVKRGHQVTVMTTQYDP
                     SLPREEVMDGVKVIRVPVLARVSKGVLAPTFGLVATKLAWEHDVVQMHLPQFDAPGVA
                     FRARLFGKPAVLTYHCDIQLPQGVFNRLVNAVVNFQNHMAGILSNHIVTYTQDYADHS
                     PYLSHYASKLTPILPPVELPDPLPGAVSAFASTHLTQERRPVIGMAA"
BASE COUNT         2480 a         2537 c         2538 g         2739 t
ORIGIN      
        1 cgttcacgtc gatccatttg gcgagggcgt cgtaggcttc accgatggtg gcaaatgacc
       61 cggcatggac gacgcacgcc atcgtttcca ccgcaggcag gtcatagact ttgacccgcc
      121 cgacgggagt cgggtcacca gcgatgggca tggcaacttc aatatcccaa tcaccttcgc
      181 gtgcatcggc atcgtgatag atcgccatcg gtgtgccgtt catacggata ttgttttgat
      241 aaataaagcc cagtagttca tcccaaagag atcgttgcgc cggtgggttc gcaaccacac
      301 cgcggacggt ggcggctttc attgaatcta ttttcttgat aacgacatca tacttggaca
      361 taaagttctc ctgttgagtt tgtctcagcc agcgttccac ttgctccagc cttgccgctt
      421 cttcttccac tcgctgccgg atttccgcct gacggagttt gagcattcca cgtaactctt
      481 cggtggaaag attctcttcg agaaaacgac cgatctcctc taaagagaac cccaactcct
      541 tcaatgccag aatgcgatac aagcgcggca gttggtgaaa gtcgtaataa cggtagccag
      601 tgaagcggtc tacctcaacg ggtttgagta aacccatctc gtcgtagtac cgcagcgttt
      661 tgatggaaac gcggctaagt ttggaaaagt ctccgattcg gatcatacgt aggcgccttc
      721 attctcttat acaccctcca gtaacgggag agtcaagtgc tgggcattag gacttatatc
      781 ccagcatgtt ttttggtaga tattctataa tcacagcatg gattctaaac ctacattcga
      841 taacttcccg cttctttcta ccccgcgcct gctcttgcgt gacctacgcc cgaccgacct
      901 cgacgatctg tacgagtacg cctctgaccc tgaaattgac cgttacactc catgggaccg
      961 ttatcagtct ttggatgatg cgcgtgaaga cttgggtggt tatattgccc gctacgaaca
     1021 gggacatttc cgcgcctggg gcatcgaaca ccgcgccgat aaaaagttga tcggcatcac
     1081 caacttcggc tttctctcaa aagaagaccg ccgcgccgag atggggtaca ccattgcgcg
     1141 caaatattgg gggcagggct tcgctactga agcggctcaa gcgttgataa ggtatgggtt
     1201 cgagaaactg gacttagcgc ggatcgaggc ggtagtcctt ccagaaaata aagcgtcttc
     1261 caaagtcatg ctcaaagttg ggatgcagta tgaaggtcta ctgcgcagtt atcaagtatg
     1321 gaaaggcaaa cccagcgatt tggaaatcta tgcgattgtg aaggcataag atggctatta
     1381 ttcttgaaac tgagcgtctt attcttcggc atcaagttct ttcagacttg gatgatcttt
     1441 gggcgttgta tcaagaccct gaaatcacaa aatacattcc cgatgcgccg cgttcgcgcg
     1501 aagaagcaca gaaagaactc gaatggcata tgcacggaca tccgcgccgc cccgagttgg
     1561 gtttgtgggc gaccattcac aaagaaacgg gcaagtttat cggtcgttgc ggacttttac
     1621 cttgggagat cgacgggcag aacgaggtcg aagtggcata tacgatcgct gaggcgtatc
     1681 aaggtcaagg cttggggagt gaggcggcgt tagccatcct gaactacggg ttcagtaaat
     1741 tgaaattatc ccgccttatt tgcttgatcg acccggagaa tatcccttcg caaaaagttg
     1801 ctgaaaaaat ggggatgcgg ttcgaaaaag aaagccggga tgaatacgga atgttttgga
     1861 tatattcaat aaaaaaagaa gctggcaact aattgccagc ttcttttttt atgacttagg
     1921 cttttttcgt gtcgtctttc ttttgcatgt ctttcaaaat cttctcgcgc atggcttcca
     1981 catccggtga gcgttgggtt gtgctggctt caccagagac gccaaagcgt tcggagagca
     2041 acatggtcag caagccttcc atgatgttgg cagattggtt tccgccattc tcgccattgc
     2101 tgccaccgac caccacacgc ggaaccacat ccacaccaga ttgttgaatg gcttcagcaa
     2161 agcgattgag cacttgctgt gtcacctgga actgcggacc accatacgcg cgcacctgtt
     2221 cttcggtggc aagcgcttgc gcgataccga tacgggcaac cttttcggct tcggcttctg
     2281 ctaaggcgcg gatctgttgc gcctgctgga tggagcgttc gtactctgcc ttaccctggt
     2341 tcgactggat gctaatgctc aactcggctt ccgtcatctt ggtctgcatc gcagcacggg
     2401 attcggcttc acgtagttcg cgttccttca ccgcggcttt ttcttgttgc gagtaggttt
     2461 ctacctgctc ttccgcgatc tggcgctgac gtaactgagt gaggatggtt tcaatctgtt
     2521 tgtcacctgc cggtgaggaa ggtgtaccga ttagcacttc ttccaactca aggttgtagt
     2581 gttcaaactt ttccttcatc tccaggctgg attgttgctg aatggcacta cgttcctgca
     2641 agagttgaat gagggtacgc gtctgggcaa cgttcttgaa gtatgcggcc accatggggt
     2701 cgagggtttg ttcaacgagg cgtttgatat cgccgaagcg ttgaatgacc agcggagctt
     2761 tcttatagtc gatgtgaacc acgactgaaa gcggcagaga aggctcgaac gcgtccttgg
     2821 tgatgagcga gacttcggtc aggttctcat cgaagttgtg cgaaccgact tcgttcttga
     2881 tccacttcaa aatgaagtta gtggttggca cagggatgac cttgcctgca taggtattga
     2941 acggatactt acccggtaga agcgcttcac tccacacgcc acgttgtcca cgggtgacga
     3001 gttcaccgtg tcggtattct gaaccggaaa ggtcaacgcc ttgttcacca gtgtaagaaa
     3061 ccaccacacc tacattccct acgtcaatga tggtcttgtg gatcatctca acagtggcga
     3121 acaaacggtt gatgtaatac gtaccttcca ccaacacttg gagttggcga ccacgcatac
     3181 catttgcttt caagaagcgg tcagcatctt ggaacttgtt gtggtattca tcggctttct
     3241 ctgggttatc acctaccact ggggcaatga tctcaccttg cggtaaagac ggaccatcat
     3301 gtacggtcac aataccgatc ttatcgtcac cgtccttgat gatgactggg cggaacccat
     3361 cacgttcttt gatcacttct gccatgcgca cgatgacagc cgtatcgtct cggctcaacg
     3421 gcagagagta cacgcgctct tcagtaatga ccacgaactg catcaagttg aatgcatatg
     3481 taccttcacg caagatcaag cgttgcggac ctttttgccc gccatttttc aagaatgtgg
     3541 tcacatcctg aaaatcgtgc gcggttacat tggaagccaa gacctgtgcc ggttcaagcg
     3601 gagcaccgtc gcgcgcgaaa acgtacccga ttttgccttg ggtaatggtc accaagggtg
     3661 caatgtggat cgcatactga atgggcatgc ggtaatgaag cccaccacgc aacacctgtg
     3721 gctggaaacc cgtctcgcca ttaatggcga tcaagccaga tttcaacgac tttccaccga
     3781 agcgtttttc cacgataccg atacggttgt ttggaataaa gcggataccg gtcagtaaga
     3841 ccaaaaaaag aacgccaagc ccacctaaac ataacgttac aagaatattc attttttctc
     3901 cttcaccatc taaatacgct gtgaggcgta ttcaggtttc caattttaga tatggtggcg
     3961 tccgagtacg atacctgtgg aaataatcac aaacaggatg gagacaacca gcacaaggat
     4021 gcccaacagc cacgaactga tataaattga aaggaacccg gttaatacga caatggtcag
     4081 gaataggaac tgtttgaaca catccgcgcc tcgttttact ggaccttgga actgatgcac
     4141 cccataaagc ccgattccca tcccaagcag gctcaatata acgaagcccc aaacgattat
     4201 agtcccgtta ccgggggcgt ttagcacaaa aataccgcag gtcgtttcac ggtcagggaa
     4261 aggataggaa gaatagacaa gtgccttcgc aaaaatgaag cgcttcaagt ccaaattttc
     4321 gggtccaacc gtccattcag cggtcgtttg ttcgcctgct gcaagctcaa ggttttctgt
     4381 ggtttcaaac gctgccaccg gcgaactggt ttctaccaga attgtcgggg agattttccc
     4441 atcggtggtg ttcttgagcg taagcgaaac cgtgttcgtt tcagttgccg tcatgagaac
     4501 cgggcagcta aacccgctca gcggtgtgct ggcacggcgc gagaacccat aataagcagc
     4561 ttccaggtct gcccaggtgg caagtgtgat caagaccaac ccacttagaa ccccaagtgc
     4621 gaacataata aagaattgat tgtatgattt tgattgattc tgcatgtgat ttcctttaaa
     4681 ggcgatagtg attctatacc ctgtctcttg ttttgccatg gtgatttagt agggttttcg
     4741 ccgcgttgcc ctcaaagatt ccgtctactt atcgggtata cttcgttcgc ttgttgggta
     4801 aaaataccgc caaccaaaac attttataga aaaattgaaa gtagtatgac cgaccatatc
     4861 cgtaattttt gtatcatcgc ccatgtagac cacggcaaat ccacgctggc agaccggctc
     4921 ctgcaattga caggcaccgt ctccgagcgc gatatgaccg accaagctct cgactcaatg
     4981 gacctcgaac gcgaaaaagg cgtcaccatt aaggcgtctg ctgtgcgtat gttctacacc
     5041 gccaaaaatg ggcagaaata cgaactaaat ctcatcgaca ctcccgggca tgtggacttc
     5101 ggctatgaag tcagccgcgc gctaaaagcc tgtgaaggtg ccattcttgt tgtggatgcc
     5161 actcagggca ttgaagctca aaccttagcc aatctctacc aagcgcttga tgccgacctg
     5221 acccttgtcc ctgttatcaa taaaattgac cttccctccg cccgtcctga cgaagtagca
     5281 gaagatgttg gttcgcttct cggcatcgac cccgattcgg ttctgcgtgt ctctgctaaa
     5341 gaaggcatca atgtagacca aatcctcgaa gccatcgtgg agcgtgtgcc cgcaccgaaa
     5401 gatgccgacg ccgaacccct gcgtgctctc gtctttgacg cgcattatga ctcgtataaa
     5461 ggcgttattg cttacatccg tgtgtttgaa ggcaagatca aaacgaatga tgtcttgcgg
     5521 atgttcgcaa ccagtaacga cctgcgccct gttgagatcg gaatttttgc acccggcatg
     5581 aaacctgtgg atgtaattgg ctcgggcgaa gtaggctatg tagctaccgg cttcaaaacc
     5641 gttcacgaat gccgcgtagg tgacacgttg actctggctg cctctcccgc cgccgaacct
     5701 ctgcccggat acttcctccc caaaccgatg gtctttgcgg gtatttaccc cgtcgaagcg
     5761 gatgattact ctgagttacg tgaagcgctt gaaaaacttc aactcaatga cgcttcattg
     5821 acctatcaac ctgaaacttc gcaagcctta ggctttggtt tccgcgcagg cttccttggc
     5881 ttgttccata tggaaatcat tcaagagcgc attgaacgtg aatacttgct cgatgtgctt
     5941 ttcacggcgc cctctgtaga atacgaagtg ttgaccattg gcgatgaagt cgtcaccgtg
     6001 gattcacctg ccgaattgcc agaccccagc aagatcgtcg aagtgcgcga gccgtggatg
     6061 aatatcgaga tcatcacgcc gaccgattat tacggcgcca tcatggactt ggtcaccaaa
     6121 cgtcgcggca ttttcaaaca gcaagaacac cctgctccac atcgcgtgca gttggatttt
     6181 gaaattccgc tctctgaaat cattgtggat ttcttcgacc acttgaaatc gcgcaccaag
     6241 ggttatgcgt ctcttgatta tcaattcctt gagtaccgtg ccgacaaact gcaaaaactt
     6301 gagatcctcg tcaatgggga tacggttgat gcattggcag ccattgtcca tgagaaggat
     6361 gcgtaccata aaggtcaacg cctgattacc aagctcaagg atttaattcc gcgccagtta
     6421 tatgatgtcg ccgtgcaagc cgcttctggc ggacgtatca tcagccgcgc tacggtcaaa
     6481 gccacgcgta aagatgtgct cgcgaagtgt tatggtggtg atatctcacg taagaagaaa
     6541 ttgcttgaaa aacaaaagcg cggtaagaag cgcttgaaga tggtgggaaa tgtcgagatc
     6601 ccgcaagaag cctttatggc ggtcttgaag ttggaagacg aatagtgtat gcggattgca
     6661 cattatcata tgtaatgtgc aatccgcatt ttgtaaggaa cccccatgtc tgaaactcaa
     6721 aataatctaa aaagaatcct ccctggcgtt ttggttagca ttgcgctcat tgcagccatt
     6781 ctctactttg tggatatcaa gaccaccttg aatgccttgc gcaatgcaaa ttatggactg
     6841 atcggcatcg ctgccatttt ctcttttatc tggcttgcgg ttcgtgccgt tgtatggcgc
     6901 acgttacttc gcaacaagcc aacttataaa gatgtattct ttaccgctgg cgaaggctac
     6961 ttgctgaata acttcctgcc atttcgcttg ggcgagatcg gtcgtgcctt tttgctaagc
     7021 cgtaaatcag gcggcatgac ctttgccgaa attatcccaa ccatcgttat cgaacgcgca
     7081 gtggatttga tcatttcagc agtcatcttc ttggcggctt tgccgtatgt ggtcaatgca
     7141 gagagttcgc ctacacttgg gtacatcctt ggtggggtca tggtgttcgg cttggtcttt
     7201 atgtacatcc ttgcccgtaa caatcaatgg gcgttggatt tattccacaa actgagcggg
     7261 cgtttcccag ccttgcaaaa agttggcggc agtttccttg agtccttctt ccaagggctt
     7321 ggtgtgctta ccgatggctg gctcttccta cgctttttat tttggatgat cctcaattgg
     7381 gcaattgcct tgtttgcata ttacctgatg gtccttgcct ttttcccaga agctaaaccg
     7441 atctgggcga tcttcgggct tgggatggcc gccttcggcg gcgcggttcc ttctgcgccg
     7501 ggagcggttg gcaccttcga gggagctgtt gtcttcgccc taacccgctt caccagcgat
     7561 gaatctactg cgtttgcagc agcacttacc atgcgtattt ataactatct caatagtact
     7621 gttgtgggaa tgctcgggtt atcgagcgaa gggcaaaccc tttcaggtgt ttacaaacaa
     7681 ctaattaatt ttaggaacag aaaaacagat gaagaataaa cagtatctcg ttacaggtgg
     7741 tgccggtttt atcggttcaa attatgttca tcgcttgcta aaacgcggtg aaaaggtgac
     7801 catttacgat aacttctcgc gtggcggggc tccgcgtaat ttggaatggt tgaaagctga
     7861 gtttggtgag aaggctttcg aggttgtggt tggggatgtg cgagacgctg ccagaatcgc
     7921 tgaggcagct aaaacgtcag atgtgatcgt gcatttggca ggtcaagttg cagtgaccac
     7981 ttcggtcacc catcctagag atgattttga agccaatgcc ttagggacct tcaatgttgt
     8041 agaagccgca cgcgcgtcgg gacgtcagcc tattgtgatc tatgcttcca cgaataaagt
     8101 gtatggcggc atggaagaag tagagctatt tgaagaacct actcgttggc gttataaaga
     8161 tttgatccat ggttgccctg aaactcagcc tttagatttt cactcgcctt atggttgctc
     8221 taaaggcacc ggcgaccaat acgtgcgcga ttatgctcgt atctatgatt tgccaacggt
     8281 agttttccgc cagtcttgta tttatggtcc gcgccaattt ggcattgaag atcagggttg
     8341 ggtggcttgg tttattattg ccgcggttat gggacggaac atcacgattt atggcgatgg
     8401 aaaacaagtg cgcgatattt tgcatgtaca tgaccttatc caagcgtatg accttgccgt
     8461 tgaaaagatc gacgtggctc gtggacaagt ttataacctt ggcggcggac cttcaaatgt
     8521 gatgtcgatc tggtcagagt ttggtccgcg tttggaagcg ttgcttggga agaagatcga
     8581 agttgggcgc ggcgattggc gccccggcga ccaacgggtc ttttatgccg atgtttccaa
     8641 agccaagaac gaacttggtt gggagcccaa gatcagcgtg gaacaagggg tgaacatgct
     8701 gtttgaatgg gtgcaagcaa ataagaatct cttctaaagt aaatatcaaa cggctgtaaa
     8761 agttcaaccc gaatttttac agccgtactt tattaggtaa aatacctagg ctagattttc
     8821 cattcaagaa aagaaaccca tccttatatg cctcaccaac ttttatcttt agtcgatgtt
     8881 gccatggcaa cactgcccaa tgttttaatt tcgctggcaa tttttgctgt gagttattat
     8941 gccggcgttt ggctgggtcg tttactaaag ggcgtgctca cccggaagaa tgccgagcct
     9001 ggcgtttcgc atttgctttc acagatcctt aagtggacga ttatttctct cggcttcatc
     9061 actgctttgc aaaggttttt tgatgttacc gccttcttaa ccggtctagg catcattggc
     9121 tttaccattg gctttgcctt gcaaaacatc atgcagaatt ttgtctctgg cattattctc
     9181 ttgatccaac agccgtttaa agtaagtgat gaagtgagct tgatgggaaa cgaaggcttt
     9241 gttcagaaga ttggtttgcg caccacagaa atccgtttgc gcaatgggtt gctggttttt
     9301 ctacccaatg ccgatgtgct ttctcaacca atcatcaact ttagccgggc aaatggcttt
     9361 cgtgtggaat tgccggtaag cattccctat gaagccgata ctgaaaaggc gcgttccatt
     9421 attcttgacc aagttagaac cgtgcccggt tgtttggaaa cacccgttgc ccaagtattt
     9481 tttcatacct ttaacgcctc gtcggtcgat ttacgcgtac agttctgggt ggataatcgc
     9541 actaccgacc atcatgcagc gaaagatatg gtgttggtca gaatcaaaca agcatttgaa
     9601 gaaaacggga tcgacgttcc gtacccagtt caagttatga aaaatccaac caaagtgacg
     9661 aaacgaaaga caaaatgaag atactgaccg tactaaccta ttatcgccca catacctctg
     9721 ggctgactat ttatgcagaa cggcttgcac atgcctttgt caaacgcggg caccaagtca
     9781 ctgtaatgac cactcaatac gacccatccc tgccacgtga agaagtgatg gatggtgtaa
     9841 aagtgattcg tgttcctgtg cttgcgcgtg ttagcaaagg cgtgcttgct ccaacctttg
     9901 ggttggtcgc taccaaactg gcatgggaac atgatgttgt tcaaatgcac cttccgcagt
     9961 ttgatgcgcc cggcgttgcc ttccgtgcgc gtttgtttgg caagcctgct gtccttacat
    10021 atcattgcga tattcaactt ccgcagggcg tattcaatcg tttggtcaat gctgtggtga
    10081 attttcaaaa tcatatggcg gggattcttt cgaatcatat cgtgacgtac acacaggact
    10141 atgcggatca ttctccgtac ctctcacatt acgcctcaaa actgaccccc atcctgccac
    10201 ccgttgagtt gcctgatcca ctccctgggg ctgtttccgc ttttgcatcc actcatctta
    10261 cgcaggagcg tagacccgta attggtatgg cagc
//
LOCUS       DAOQNJ010000020        10275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3067_length_10275_cov_10.958415, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000020 DAOQNJ010000000
VERSION     DAOQNJ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10275
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3067_length_10275_cov_10.958415"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLG52_01220"
     CDS             <1..519
                     /locus_tag="PLG52_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB40086.1"
                     /translation="ETSGIDAMWRYCEGFRAGVKHVSPELQALVVYREDGDREKLFID
                     EQWGYDTAKNLIERGADVVFAAGGVTGQGALKAATELHVYAIGSERDQAAVLGVQGSS
                     VVTSVYGQSQLTVQDVMRMLREGKLSKTWQGSVGYIPLSPVYPPEVSLGLDLLLGALK
                     RGEIQTNVTKSH"
     gene            628..1764
                     /locus_tag="PLG52_01225"
     CDS             628..1764
                     /locus_tag="PLG52_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB40087.1"
                     /translation="MKKLYFVMAALIIASMVLAACGTPAATEAPAATEAAATEAAATE
                     APAATEAAAATIKVGEVTDLGGIDDKSFNANGWAGAQKAAAELGVEAKYLESTQQADY
                     AKNIQQFVDEGTNLIVTVGFLLGVDTATAAKANPDTHFAIVDYAYPDCFGTQVEGKDC
                     GSATELPNVLGLTFQIDQAGFLAGYAAAASTETGKVATFGGINIPPVASFMIGFEKGV
                     LYYNEQNGTAVEVLGWNTEANDGSFTGNFDSLDDGRSFAESFVQEGADIIMPVAGPVG
                     LGSAAYCQESGACKIIGVDVDWTVSAPEYKDIILTSVLKNINVAVYDAIQAEVDGTFA
                     GGVYVGTVENGGVGISDVAGASDDLKAQLDAVTQGIIDGSIATR"
     gene            1956..3488
                     /locus_tag="PLG52_01230"
     CDS             1956..3488
                     /locus_tag="PLG52_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40088.1"
                     /translation="MTNVLELRGITKRFPGVLANDKISITLREGKILALLGENGAGKS
                     TLMNILYGLYKPDEGQIIVRGKEVDIQGPNDAIAQGIGMVHQHFMLVPVMTVTENVML
                     GIEPTKNGIFLDKEKVANRIREISDEYGLEVDPNAYIKDLPVGIQQRVEIIKVLYRHA
                     DILILDEPTAVLTPQEVEGLFKIIDTLIKAGKSIIFITHKLKEVLAVADDITVLRLGT
                     VVGSIDPKEATSEILATMMVGRDVTLVVQKKLSEPGRAVLDVKDLYVRDERQNMTVQG
                     VSFEVRKGEVLGVAGVQGNGQTELVYALTGLLPLEAGTIHLLDEAIHHTSPRHILEKG
                     VAHIPEDRQRHGLILSFPIHDNMMLCTYYKDPFAKGISLQQKTIFENADQLVKQYDVR
                     TPNIYVNAGTLSGGNQQKVIVAREFSRPIELLIASQPTRGLDVGSIEYIHSQIIKKRD
                     EGSGVLLVSSELDEILALSDRIAVMYKGQIMAIVDASKATKEQLGLLMAGVHPSETSN
                     KN"
     gene            3500..4687
                     /locus_tag="PLG52_01235"
     CDS             3500..4687
                     /locus_tag="PLG52_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40089.1"
                     /translation="MTTTATPAPDAGTKETKRNSLWQELLIPILAVVTGLIVGAIVIF
                     VSGDNPFVAYGALFAGSFGTPADYVEGFKVLFSTGDTAALIKAIYPFTESLVTATPYI
                     FAGLSVALGFRCGLFNIGAEGQFFIGALCSAFVGYSLKGLPMIVHLPLAILGGALGGA
                     IWGAIPGYLKARFGAHEVVNTIMMNWIAFRLSDWLLSGPMKSSGFRPVTPNVEASAEL
                     PRFFPEPLRFNLGFFLALLAAYLLYWFLFKTTLGFEIRSVGANPDAAKYAGMNIIRNF
                     VLVMFLAGGLAGLAGTAQVLGVDHWVGQGFSAGYGFDAIALALLGRSHPAGVVLAALL
                     FGFLRSGATDMQSMAGIPIDIISIIQGMVIVFIAAPDIIRWLYRIRATKKDDAVLTRG
                     WGK"
     gene            4699..5961
                     /locus_tag="PLG52_01240"
     CDS             4699..5961
                     /locus_tag="PLG52_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40090.1"
                     /translation="MKNTKPEISSNTEKRQRISYGLVLLAVGLFIYFVFGLQTEAGTV
                     AKFGLNLAGSQAIEISDLSVPALPVVYFMAFLAVFAGAFQLARGVRSTNWLVGILAVT
                     IVVSFLMWAAQDKSFNLTGMLSSSFVRATPIALAALCGVISERSAVINIGIEGIMLIS
                     AQLAVLTATVTKSLYVGLFAAIIAGGLIAALHAYLVIRFKVDQTVSSVAINIFGAGTT
                     AFISSRFMQRAGDQLNNSGTFPIISIPGLSKIPVLGPVLFENNLIIYLAIILVFVMHI
                     VLYYTPWGLRTRAVGEHPKAADTLGINVYFTRYINVIAGGMIAGLGGAYFTLGSVGRF
                     DEAMTAGKGFIGLAAMIFGNWNPIGAYTSSLIFGFADSLQVKLQILRIPIPSEFLLMA
                     PYIVTMIILTGVVGRAIPPAADGQPYEK"
     gene            6094..6999
                     /locus_tag="PLG52_01245"
     CDS             6094..6999
                     /locus_tag="PLG52_01245"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB40091.1"
                     /translation="MKNLTRLFHLFVVCALFLGACNLPSNNPQGAAATYAAQTVAALL
                     TPSTTPTTSLTSNNTATPSFTPSPTNTSTPAATATPTCPQAQFVTDVTIPDGTVMTPG
                     QSFTKKWRIRNTGECAWNGYNLVFDTGDSMGGPASKAVGVVNPGQEIDIEVNLTAPAS
                     AGNYRGYWRIVTNNNVLVPMLNGYQGRAFYVDIKVQAPATATNTSAPAFAVTSVVFEV
                     TGTCPNFHVKVSVTTSAAGTVKYKFVSQDGSSTSLADLVFAAAGTKVVEYDRFFGVSP
                     GSLWTDMYVDQPNNQQFGRATFVCP"
     gene            7088..8047
                     /locus_tag="PLG52_01250"
     CDS             7088..8047
                     /locus_tag="PLG52_01250"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB40092.1"
                     /translation="MLKKKIYTLSALFFTAALLLTACGGNVDAETAIQTSVAETVAAQ
                     TPIVITETPAATSFPTQTPFTTPTLGTPIALVSPTTGAVTGGKAECAKASLQDETVVD
                     GKIFKPGEEFVKTWYIKNESTCVWDTTYKIIFWSGDILGGGYYYQLPQVTGPGQTVPV
                     SLVLTAPTTPGEYRSEWKLQTPDSIPYGVGVYQAAFYTEIEVANLTTTTAITYGIVDA
                     NLVITRDPPSGCQPANMVYTANVSITTNGPLTFKYTIRQQDGNSGNSTKVVMKEAGEY
                     TNSDHVWKLGRAASQNSNRWMQLVITDPVYKEYPIVPFDFYCP"
     gene            8148..9404
                     /locus_tag="PLG52_01255"
     CDS             8148..9404
                     /locus_tag="PLG52_01255"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB40093.1"
                     /translation="MSKKYSPFILLLIVLLTVQLACNLPSDSATPDPFATLNGLYTAS
                     AQTQAVAGSPTPGLPQPTVTLAGSPTATNQPVTLQPAQTSRCDAAQFLADVTYPDGSL
                     VPRNTTFVKIWRIRNVGTCTWSTSYSVVFTGGDGMGASTSIGMPGSVAPGQYIEIPVT
                     MVSPSKDGNYRGYWKIRNSSGALFGIGGQADTAFWVDIRVTGPSFVAYNFAENYCQAN
                     WSNGSNALPCPSSNGDANGYIIRLNAPVMENGVTEDEPGLLTVPQDKNNGIISGQFPS
                     FTVQSGDRFRTIVNCQRDATKCNVVFRLDYLNNGVTSTYASWAEAYEGKYYPVDLDLS
                     SLAGQTLKFILVVSANGGNNQDFAIWLNPHIVRQGVAPTSTNTPTPTITFTPTLTFTP
                     TITSTATTTSTPTVTATQTETPTPTP"
     gene            9543..>10275
                     /locus_tag="PLG52_01260"
     CDS             9543..>10275
                     /locus_tag="PLG52_01260"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB40094.1"
                     /translation="MNFRKFRWLMISTLAAVLVSSCNFGATPPPTQDTGAIQTQAFGQ
                     VLTQVASQATQTAAAIPPTPLPTNTPVPTNTLPPIPTSDAFNIPTNTPFVFNTQQPGL
                     TPQSGLSFTATVGVYSTTTTKNGCSDGYLISESQPYDGKTISANTEFTKSWDFINTGT
                     CAWDEGYTFAFVQEFSNGPIYVLPKKDYVIPKTGPFVEPGKTGTFSVPMRAPKDPGEY
                     TWYFKLRDDSGQYFGSLVWVKIISVR"
BASE COUNT         2537 a         2609 c         2428 g         2701 t
ORIGIN      
        1 gaaacctctg gcattgatgc catgtggcgc tattgcgaag gctttcgggc tggcgtgaaa
       61 cacgtcagcc cggagttgca ggctttggtg gtgtaccgtg aagatggaga tagagaaaaa
      121 ctgttcattg atgaacagtg ggggtatgac acggcgaaaa atctaattga acgcggagcc
      181 gatgtggtct ttgccgcggg cggagtcacc gggcaggggg cgttaaaagc cgccacagag
      241 ttgcatgtgt acgctatcgg gtcagagcgc gatcaggcgg cggttttagg ggtacaaggc
      301 tcgagcgtgg tcacttcggt atatgggcag agtcagttga cggttcagga tgtgatgcga
      361 atgctccgtg agggaaaact ctctaagaca tggcaaggct cggtggggta catcccgctg
      421 agtccggtat atccgcctga ggttagtttg gggttagacc tcttgttggg ggctttaaag
      481 cgaggggaaa tccaaacaaa tgtcacaaaa tctcattaac attgcgcagt cttgcacaaa
      541 gattggagta gatttatact tgttaggtcg tgaggctgta cgaaaacagt ctttccaatc
      601 actatcttct agaggagaag acataaaatg aagaagcttt attttgttat ggctgctttg
      661 atcatcgcct cgatggtctt ggctgcctgt ggcactcctg ctgccactga agcccccgct
      721 gctactgaag ctgccgctac cgaagctgct gctaccgaag cccctgccgc taccgaagct
      781 gccgctgcca ccatcaaagt tggtgaagtg accgacctcg gcggtattga tgacaagtcc
      841 ttcaatgcca atggttgggc tggcgctcaa aaagctgctg ccgaactcgg tgtggaagcc
      901 aaatatctcg aatccaccca gcaggctgat tacgccaaga acattcagca gttcgttgac
      961 gaaggcacta acttgatcgt caccgtcggc ttcttgctcg gcgttgatac cgctaccgct
     1021 gccaaggcga accctgatac tcacttcgcg atcgtagact atgcttaccc tgactgcttc
     1081 ggcacccagg ttgaaggcaa ggactgcggt tccgcgaccg aactccccaa cgttctcggt
     1141 ttgaccttcc agatcgacca ggctggcttc ctcgctggtt acgctgctgc cgcctccacc
     1201 gagaccggta aagttgccac cttcggtggt atcaacattc ctccggttgc ttccttcatg
     1261 atcggtttcg aaaagggtgt tctgtactac aacgaacaga acggcaccgc tgtggaagtc
     1321 ctcggctgga acactgaagc caacgatggt tccttcaccg gtaacttcga ctccctcgat
     1381 gatggtcgct ccttcgccga atccttcgtt caggaaggcg ccgacatcat catgcctgtg
     1441 gctggacctg ttggtctcgg ctctgctgct tactgccagg aatccggcgc ctgcaagatc
     1501 atcggcgtgg atgttgactg gaccgtttct gctcctgaat acaaggacat catcctcacc
     1561 agcgttctca agaacatcaa cgttgctgtg tacgatgcca ttcaggctga agttgacggc
     1621 acctttgctg gtggcgtgta tgttggtacc gttgaaaacg gcggcgtcgg tatctccgat
     1681 gttgctggtg cttctgacga cctcaaggct caacttgacg ctgtcaccca gggcatcatc
     1741 gacggctcca tcgcaaccag ataatctggc tctacttgca ctacaaaagc cggaggaggg
     1801 gtttccctcc cccggctttt tatttttctt attgctcgta gatctcaaag gttttttcaa
     1861 tttgtcatac attttccatg caccatcctt tataattggg gcgtgcaaaa aaaacctttt
     1921 ggatctatca aagtaaagat agtggagggt atggcatgac caatgttttg gaactgcgtg
     1981 gcattaccaa acgtttcccc ggtgttcttg caaacgacaa aatcagtatt actctgcgtg
     2041 agggcaaaat tctcgctttg ttaggtgaga acggtgcagg caaaagtaca ttgatgaata
     2101 tcctttatgg gttgtacaag cctgatgaag gacagatcat tgtccgcggt aaagaggtgg
     2161 atattcaagg acccaacgac gccatcgcac aagggatcgg catggtgcat cagcacttta
     2221 tgctcgtccc tgtgatgacg gtcaccgaga atgtgatgct cggtattgaa cccacaaaga
     2281 acggtatatt tttagacaaa gaaaaggtcg cgaaccgcat ccgtgaaatt tcggatgaat
     2341 acggtttgga agtagacccc aatgcctata tcaaagatct gcctgtgggc attcagcagc
     2401 gcgtggagat cattaaggtt ctctatcgcc atgcagatat tctcatccta gacgagccga
     2461 cagcggtact caccccgcaa gaagtggaag gcttgttcaa gatcatcgat acgctcatta
     2521 aagcgggcaa atccattatt ttcattaccc acaaattgaa agaagtactg gcagttgcag
     2581 atgatattac tgttctgcgc ctcggaactg tggtcggatc gattgacccg aaagaagcga
     2641 cttctgagat tttggcgacc atgatggtgg ggcgtgacgt gaccttggtg gttcaaaaga
     2701 aattatctga acccggcaga gctgttttgg atgtcaaaga cttgtatgtg cgagatgaac
     2761 gccaaaacat gaccgttcaa ggcgtatctt tcgaagttcg taaaggcgaa gtccttggcg
     2821 ttgctggtgt tcaagggaat gggcaaacgg aattggtcta tgccttgacc gggttgttac
     2881 ctttggaagc agggactatc catttattgg atgaagccat tcatcacacg tctccacgtc
     2941 acatccttga aaaaggcgtg gcgcacattc cagaagatcg ccagcgccat ggtttgatcc
     3001 tttccttccc catccacgac aatatgatgt tatgcaccta ctacaaggac ccatttgcaa
     3061 agggcatttc cttgcagcag aaaaccattt ttgaaaacgc agaccaattg gtaaagcaat
     3121 atgatgtgcg caccccgaac atctatgtca atgcagggac gctttccggt ggtaatcagc
     3181 aaaaagtgat cgtggcgcgt gaattttcac gtcccattga attattgatc gcctcacaac
     3241 cgacccgtgg gttggacgtt ggctcgattg aatacatcca ctcacagatt atcaaaaagc
     3301 gcgacgaagg ttctggcgtt ctgctcgttt catccgaatt ggatgaaatt ctcgctctct
     3361 cagaccgtat cgctgtgatg tacaaaggtc agatcatggc gatcgttgat gcaagcaaag
     3421 cgaccaaaga acaacttggt ctgctcatgg cgggtgttca tccctccgaa acctcgaaca
     3481 agaattagga gttgtatctg tgactactac agctactccc gccccagatg ctgggacaaa
     3541 agaaacgaaa cgtaattcgc tttggcagga attgttaatc cctattcttg ctgttgtgac
     3601 cgggttgatc gttggcgcca ttgttatctt cgtcagcggc gacaatccct ttgttgcata
     3661 cggtgctctc tttgccgggt ctttcggtac ccctgccgat tatgttgaag gcttcaaggt
     3721 tttattctcc acaggagata cagccgcatt aatcaaagcc atttacccat ttactgaaag
     3781 tcttgtaacg gctacgccct atatttttgc agggctttcg gtggcactcg gatttcgttg
     3841 tggtttgttc aatatcggtg ctgaaggtca gttcttcatt ggtgctcttt gctctgcctt
     3901 tgttggctac agcctcaagg gacttcccat gatcgttcac ttgccgcttg ctattttagg
     3961 cggcgccttg ggcggagcaa tttggggagc cattccgggc tatctcaagg ctcgcttcgg
     4021 cgcccatgaa gtggtcaaca ccatcatgat gaattggatc gctttccgat tgagcgactg
     4081 gctcttaagc ggaccgatga agtcttctgg ttttcgcccg gtgaccccca atgtggaagc
     4141 ctctgcagaa ttgccacgct tctttcccga accgttgcgg ttcaacctcg gattcttcct
     4201 tgccttgctg gcagcgtatc tcctctactg gttcttgttc aagaccacgc tagggtttga
     4261 gatccgctcg gtgggcgcaa acccagatgc cgcgaaatat gcaggtatga acatcatccg
     4321 taactttgta ttggtcatgt tcctagcggg tggcttggcg ggcttggctg gaactgctca
     4381 agttctcggc gtggaccact gggtgggtca gggcttttca gccggatacg ggtttgatgc
     4441 gattgcgttg gctttgctgg gtcgcagtca tccggcagga gtcgtccttg cggcgctgct
     4501 atttggtttc ctccgtagtg gtgccaccga tatgcaatct atggctggca tccccatcga
     4561 cattatctcg atcattcaag gtatggtgat tgtgtttatc gccgcacctg atatcatccg
     4621 ctggctttac cgcatccgcg ccacgaaaaa ggatgatgct gttcttaccc gtggttgggg
     4681 taaataaacg aggcttgcat gaaaaacacc aaacctgaaa ttagctctaa tactgagaaa
     4741 cgccagcgca tttcatatgg gcttgtattg cttgcggtgg gtctgttcat ttactttgtc
     4801 tttggtttac agacagaagc tggcacagtt gccaaattcg gtttaaattt agcaggctca
     4861 caagccattg aaatttcaga ccttagcgtt ccggcattgc ctgttgtgta tttcatggca
     4921 tttctcgctg tttttgccgg tgcctttcaa ttggcacgtg gggtgcgctc tacaaactgg
     4981 ttggttggaa ttcttgcagt taccattgtc gtttcctttt tgatgtgggc agcgcaagat
     5041 aaatccttta acctgaccgg tatgttgagt agttcattcg tccgtgctac accgattgct
     5101 ttagccgcct tatgtggtgt gatcagcgaa cgctcagcgg ttatcaacat tggtatcgaa
     5161 ggcattatgc tcatctcagc acagcttgct gtgctcactg ctacagtgac gaaaagcctg
     5221 tatgttgggc tctttgctgc cattattgca ggtggtttaa tcgctgcctt gcatgcctat
     5281 ttggtcattc gcttcaaggt agaccaaact gtcagtagtg ttgccatcaa catcttcggt
     5341 gccggtacaa ctgcttttat cagctcacgc ttcatgcaaa gagcgggtga tcaattaaac
     5401 aattctggca ccttccccat catctccatt cctgggcttt ctaaaatccc tgtgttggga
     5461 cctgttctct tcgaaaacaa tctgatcatt tatttggcga tcattttagt tttcgtgatg
     5521 cacatcgtgt tgtactacac cccttggggc ttgcgcactc gcgcggtagg cgaacacccc
     5581 aaagccgcag acaccttggg tatcaatgtg tacttcaccc gctatatcaa cgtcattgct
     5641 ggcggtatga tcgctgggtt gggtggcgcc tacttcaccc tcggttccgt aggtcgtttt
     5701 gacgaagcca tgactgcggg taaaggtttc atcgggctcg ccgctatgat cttcggaaat
     5761 tggaatccca tcggtgcgta cacttcatca ttaatctttg gcttcgcaga ctcgttacag
     5821 gtcaaactac aaatcctgcg cattccgatc ccctcagaat tcttgctcat ggcgccatac
     5881 attgtgacca tgatcatcct gaccggtgtt gtaggacgtg ccatcccgcc cgcagcggat
     5941 ggtcaaccgt acgagaaata acgcctcatt cgtacgccat acttgcctca aaaaacagga
     6001 gttttcacca ctcctgtttt ttattttggg aaataaggtg gatagcctaa aatttatctt
     6061 ggggtaaaat tttgataatt aagaggagaa gcaatgaaaa acctaacacg cctattccac
     6121 ctgttcgtcg tatgcgcttt attcttgggt gcatgcaacc tgccctcgaa taatcctcag
     6181 ggtgcagcgg caacctatgc tgctcaaacc gtcgcagctt tattgacccc atccacaaca
     6241 ccaaccacat ctcttacatc aaataacaca gctacaccaa gcttcacacc atctccgaca
     6301 aatacgagca cccctgccgc cacagcaact ccaacctgcc cgcaggctca atttgttaca
     6361 gatgtaacca tcccagatgg gacggtgatg accccgggac aatccttcac gaagaagtgg
     6421 cgtattcgta atacgggtga atgtgcttgg aacggctaca accttgtctt tgataccggt
     6481 gattccatgg gcggacccgc ctctaaagcc gtgggtgtcg tgaaccccgg tcaggaaatt
     6541 gacatagaag ttaatctcac cgcccctgct tctgccggaa attatcgcgg atactggcgc
     6601 atcgtcacca acaacaatgt tctcgtccct atgcttaatg gatatcaggg cagagccttt
     6661 tatgttgaca ttaaagtcca agccccagct acggcaacga acaccagcgc ccctgcattc
     6721 gctgtcacaa gtgtggtttt cgaagtaaca ggaacatgcc cgaacttcca cgttaaggtc
     6781 agcgtgacca ccagtgcagc agggaccgta aagtacaagt tcgtctctca agatggctca
     6841 tccacatcct tagctgacct agtattcgct gccgcaggca caaaagtagt ggaatacgat
     6901 cgcttctttg gtgtcagccc tggttctctt tggacagata tgtatgtaga tcagcccaac
     6961 aaccagcaat ttggacgagc aacattcgtt tgtccataaa ataataaatc attaatccca
     7021 caacgcccgt gaatacgggc gttgtcattt aacagtataa tcattttctg tatttgaaag
     7081 gaaaaaaatg ctcaagaaaa aaatctacac actctctgcg ctatttttca ccgctgcatt
     7141 gctcctcaca gcctgtggcg gcaatgtaga tgctgaaact gccattcaaa catcagtggc
     7201 agaaacagtt gcggctcaaa ctccgatcgt gattactgaa actccagccg ccacctcgtt
     7261 tccaacccaa acacccttta ctacaccgac actgggtaca cccattgcgc tagtatcccc
     7321 caccaccgga gccgtcacag gcgggaaagc cgaatgtgca aaagccagcc tccaagatga
     7381 aaccgttgtg gatggcaaaa tatttaaacc cggtgaagaa tttgtcaaaa cctggtatat
     7441 caagaacgaa agcacctgcg tttgggatac cacctacaaa atcattttct ggtctggtga
     7501 tattctcggc ggcggatatt attatcagtt accccaagtg acaggtccgg gtcaaacggt
     7561 accggtttct ttagtcctta ctgcgccaac aacccctggt gaatatcgct ccgaatggaa
     7621 attgcaaacc ccagatagta tcccttacgg tgtaggggtt tatcaagctg cgttttacac
     7681 tgaaatcgaa gtcgccaatc ttacaaccac aacagctatt acttatggca ttgtggatgc
     7741 aaacctggtc atcactcgcg acccgccatc tgggtgtcag cctgcgaaca tggtttatac
     7801 agccaacgtt agcatcacga caaatggtcc cttaacattc aaatacacca ttcgccaaca
     7861 agatgggaat agcggtaata gcacaaaagt tgtcatgaaa gaagctggcg aatataccaa
     7921 ctctgaccat gtatggaaat tgggacgtgc cgcatcacaa aatagtaatc gctggatgca
     7981 gcttgtaatc acggatcctg tctacaagga atatccaatc gtacctttcg atttctactg
     8041 cccgtaacat aaatttctca aagaagccgg tgcataccgg cttcttttta ttgggaacca
     8101 gatccatagt tttttcgtct ttaatgattg aaagaaatgg aggaagcatg tccaaaaagt
     8161 actcaccatt catcctttta ctcattgttt tattgactgt tcaacttgcc tgtaatctgc
     8221 cctcggattc cgccacccca gacccctttg ccaccctaaa cggactctac accgcctcgg
     8281 ctcagactca ggcggtagcg ggctcgccaa ccccaggctt gccccaaccg acagtgactc
     8341 tagctggctc gcccacggcc acgaatcagc cagtgacgtt gcaaccagcc caaacgtcga
     8401 ggtgtgatgc ggcgcaattt ctagccgatg tgacttatcc agatggcagt ctcgtgccac
     8461 gtaatacaac cttcgtcaaa atatggcgca tccgcaatgt gggaacgtgt acatggtcta
     8521 catcctattc cgttgttttc actggcgggg acggtatggg ggcatctact tcaattggca
     8581 tgcccggaag tgtagcaccc ggacaataca ttgaaattcc tgttacgatg gtctcgccca
     8641 gcaaggatgg gaactatcgt gggtattgga aaataagaaa ttcaagcgga gcattatttg
     8701 gaatcggcgg gcaggcagat accgctttct gggtagatat ccgtgtaacg ggaccatctt
     8761 tcgttgctta taattttgca gaaaattatt gtcaggcaaa ttggtcgaac ggctccaatg
     8821 ctttgccctg cccaagctca aatggagatg caaacgggta tatcatccgg ttaaatgccc
     8881 ccgtaatgga aaatggcgtt actgaagatg aacccggtct gttgacggtg ccacaagata
     8941 aaaacaatgg cattatcagt gggcaatttc cttcgtttac ggttcaatcg ggagaccgat
     9001 tccgcaccat tgttaactgt caacgcgatg cgacgaaatg taatgtagta tttcggcttg
     9061 actacttaaa caatggcgtt acaagcacct atgccagttg ggcagaggcg tatgaaggaa
     9121 aatactatcc agtagacttg gatctcagca gtctcgccgg gcaaacacta aagtttatct
     9181 tggtcgtcag cgccaatggt ggtaacaacc aagattttgc catttggttg aatccgcata
     9241 ttgttcggca gggtgtagca ccaacatcta cgaatacgcc aacgcctact attacattta
     9301 cgcctacact aacctttaca cccaccatta catcaaccgc tacaactacg tctacgccta
     9361 ctgtcactgc aacacaaacc gaaacgccta cacctacacc gtaaattgat ctaaaaaata
     9421 caaaacgtca cagaagcact gtacaacctg cctctgtgac gttttgtttt gggcgccagt
     9481 cacttaaaaa acactcacat ggttgcgtta taataaagtt aaccattttt ggaggttcta
     9541 ccatgaattt tcgcaagttt cgctggctga tgatttcaac tcttgcggct gtcctcgtca
     9601 gctcctgtaa ttttggagct acccctcccc ctacacagga taccggcgcc attcaaactc
     9661 aggcatttgg tcaggttctg acccaagttg ccagtcaagc aacgcagacc gctgctgcta
     9721 tcccgcctac cccactcccg accaataccc ctgtccctac aaataccctg cctccgatcc
     9781 ctacatcaga tgcttttaat attccgacaa acacgccgtt cgtgttcaat acgcaacaac
     9841 cggggctaac cccacaaagt ggactatcct tcaccgcaac tgtaggtgtt tacagcacca
     9901 ccactactaa gaatggctgc agtgatggct atcttattag cgagtctcaa ccgtacgatg
     9961 ggaaaactat aagtgccaac acagaattta ctaaaagttg ggactttatt aacacaggaa
    10021 catgtgcatg ggatgaagga tataccttcg cttttgttca agaattctcc aatgggccta
    10081 tatatgtact tcccaagaag gactatgtga taccgaagac cggtcctttt gttgaaccgg
    10141 gcaaaacagg aactttctct gtcccaatga gagcaccaaa agaccccggg gaatatactt
    10201 ggtacttcaa acttagagat gattctggtc agtatttcgg ctcattagta tgggtaaaga
    10261 tcatttctgt aaggg
//
LOCUS       DAOQNJ010000021        10258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3070_length_10258_cov_8.405763, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000021 DAOQNJ010000000
VERSION     DAOQNJ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3070_length_10258_cov_8.405763"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLG52_01265"
     CDS             <1..579
                     /locus_tag="PLG52_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB40095.1"
                     /translation="VADLLAEGERMPVYVHVIDHPNGRVLVDTGFTELHPAIADLDPR
                     IFPLNEQNVDLASIDIVVNTHLHFDHCGGNHLFPGKPIYVQQAELDDALTKDDYTIRE
                     WVDAPGVKYMPVDGEFELLPGLRLVPAPGHTRGMQMVVVETGERPIIVGGDVAVWFGE
                     LDEPKTEGQLKVHTLNPQLVWLTHEHEPWRPK"
     gene            639..1751
                     /locus_tag="PLG52_01270"
     CDS             639..1751
                     /locus_tag="PLG52_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB40096.1"
                     /translation="MAYSIKYPRKVFIRSTFRFTIRSLFPLLAQTKITGSKNFPQNGP
                     LILVGNHTGAMEVVLMGLYSPKPVEFMGAMEMPWNGWMGKMIDLYGLIPVHRGYTSNT
                     TMKMGIDVLRQGGILGIFPEGGFWEPGKQKAQSGVAWLSYAAQAPILPIGFGDTRGKM
                     AEIFQLKRPAFEMNIGDVIPPVQLEKSAHKKEELQQAADRIMDAVWALVPEDERKRKE
                     SQPENELFTFDISIFDQHGTPIPIPADLQLTDGSWISRFAHRPNLIDSIRDYIFIPVQ
                     VLKELHRKPPAEEIYLAAHSMLEYVERDNPQYFNYRYGYKDGEVFQQSFRQLRDLMKW
                     AMENQYQVAAEARYEYTDPSTGERRVLHVPQELEQW"
     gene            1794..3149
                     /locus_tag="PLG52_01275"
     CDS             1794..3149
                     /locus_tag="PLG52_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40097.1"
                     /translation="MSNSERLSTRFKMLFSTGDLSTSIPLAILMFFQLYFLTDVARLR
                     PDYAGWAIALPRLWDAINDPLFGLLSDRLRTRWGRRRALLLFGSVPLGLSFILMWIVP
                     PFGQLGLAIYYALTFIIFDTAFTVVHVGYNSLTPELTSDYDERSSLNGYRMVFSISGT
                     LGAIILATVLGWFITDARLLFIILGVGLGLASIIPPLVVFNITHEKPADEQPEPLSWW
                     QSMQLTLSNQPFRMVMGLYLLSWTTASILAAILIYFANYRLRVPEQANYFVLLAEGAA
                     IFFIPFWVWVAKKLDKRRAFIIGTLTWIFVLLGISALPADAIGLAYVLAALSGSGIAA
                     AYVLPWSMIPDIIEHDQLQTGQRREGSYYAFASFFQKLATAFALWAMGQALAANGYIT
                     PNSSGPLPTQPDSAIQAIRFFAGPAPAVLLILAVLFAWNYPITRESHSETLRVLAERE
                     S"
     gene            3198..3467
                     /locus_tag="PLG52_01280"
     CDS             3198..3467
                     /locus_tag="PLG52_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40098.1"
                     /translation="MKEYQVATEYIVSPEENINFPENVLTAIGKLKKVKEISSSTDIF
                     QCPECGTYYFYRASYEYLVSGSGSYDEYVLTREDDEIVKKYLQDD"
     gene            3479..3766
                     /locus_tag="PLG52_01285"
     CDS             3479..3766
                     /locus_tag="PLG52_01285"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNB40099.1"
                     /translation="MIIAFKGKIFQWRGPAPYLFVAVPEKESANIKAVSKLVTYGWGV
                     IPVVAKTGKTEWKTSLFPKDGRYLVPIKMVVQKAEKLKEGDEIKLQLELNL"
     gene            3856..4920
                     /locus_tag="PLG52_01290"
     CDS             3856..4920
                     /locus_tag="PLG52_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB40100.1"
                     /translation="MHNPEIIANEKEEFKQAVLNARPFKPLYVKFKVFYGCNLKCEMC
                     NHWRETREPPVTPERLKEVINELAEMGTKKIHISGGEPMLRPQIPDFVELASSLGIKV
                     TMTTNGTLITKEKAKRLVEAGLRGVNLSIDSPIRKMHEKIRGVEGSFKLTTKAVALFN
                     RYKHKGKLTIRINTVVSRTNYETLATLPDLAHKLGADGINLIPVDDHCGEILSMRKKD
                     IEKFNNEIAPRIAERALELGLIVSDEDAFPFGRDESEVRLGRAGRYAFGFYEKHPCYA
                     PWSHTLIDFNGNVYVCCMTREKIPPLGNIIHQSFREIWEGAAYRQVRLKMHPPALKPC
                     ARCDDFIDQNKSIWETIGPY"
     gene            complement(4917..5525)
                     /locus_tag="PLG52_01295"
     CDS             complement(4917..5525)
                     /locus_tag="PLG52_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40101.1"
                     /translation="MNVLLLHGQGRTTNAMRLLGFRLGRLGHPVQYFRYYTRSETFEG
                     IVNRFVQWVNTVNDGQPYAIVGHSMGGLIARAGLPLLKNLPVHLFLLASPNRPPKLAP
                     YVQNNFLYRYLTTDCGQLVLNSNFYQKLPLPNIPATVIAGSAGPRVGWLPYGFEPNDG
                     ALSVQDASMGDDYELIQVPSIHTFIMNSKLTLHYIEQILNQS"
     gene            complement(5531..5812)
                     /locus_tag="PLG52_01300"
     CDS             complement(5531..5812)
                     /locus_tag="PLG52_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40102.1"
                     /translation="MAKCEMVVGYLVPHRCDNPALGTCVKCGRGFCDEHTNATKEGRV
                     CLACQQGLEMPVALPIAASTFTPADMVLFGSTSSFDDNDDNSDMFSDLS"
     gene            5824..6981
                     /locus_tag="PLG52_01305"
     CDS             5824..6981
                     /locus_tag="PLG52_01305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB40103.1"
                     /translation="MKIALLSEKYTPDLGGLAISTGRLGEMLAAAGHDIRLFAPTSNL
                     SPTIKQTQRSSGVNVTRFGAHKRVDNTLVDWFELIVEEHKREPFDLIHAYFLPMAGFV
                     GTYAGKYLGIPSVVSIRGNDIERAAFDPSKFSHMMYALQNASAVTTNASILAKKAKAF
                     FDREAHIIPNGIDTERFKPMEKNSVLAEALGLVDEKKKEEGKFVMGFVGELREKKGLA
                     TLLSGYAQATKTRPVSLLIVGEVREGEDKKYFEEFKSNNPQLSITVTGHVPHKDLPAY
                     YSLMDVFVHPSLRDGMPNAVLEAMACGVPVIATPVGGALDVLEDGVNGFLVDVNDAPQ
                     LAEKIAKTLKPLKELESVRRSARETVLHHCTLEKELQANLKIYANLGVTTK"
     gene            6978..8396
                     /gene="aspA"
                     /locus_tag="PLG52_01310"
     CDS             6978..8396
                     /gene="aspA"
                     /locus_tag="PLG52_01310"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNB40104.1"
                     /translation="MKHRLEHDLLGEREIPNEVYYGVHTLRALENFPISKIPISNYPS
                     LVNALACVKEACAKANLELQLLEPEKANAVIKACEDVRGGALHNQFVVDVIQGGAGTS
                     TNMNANEVIANRGLEILGHGRGEYKFLHPLEDVNMSQSTNDVYPTAVKVALRFLINDL
                     IDGMEVLRLAFAAKAEEFKDVLKMGRTQLQDAVPMTLGQEFSTYAVMLEEDQQRLREG
                     ALLIQEMNLGATAIGTGINAHPEYAALARKYLSEITGIEYITAGNLVEATQDVGSFVQ
                     LSGVLKRVAVKLSKICNDLRLLSSGPRTGLGEINLPAVQAGSSIMPGKVNPVIPEVVN
                     QIAFLVIGNDVTVSFAADGGQLQLNAFEPIIAHSLFDSLIYLRNGCITLAERCVKGIT
                     ANHNRLEEHMNRSVGIVTALNPFIGYENASSVAKEAYTSNKSVTEIVLERGLLTKAEL
                     DEILKPEVLTKPRWIKKESGQK"
     gene            8399..8908
                     /locus_tag="PLG52_01315"
     CDS             8399..8908
                     /locus_tag="PLG52_01315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNB40105.1"
                     /translation="MGVLVRSETASDIEAISQVTYAAFLGKFSDHPTEHLIVNGLRNA
                     GMLTLSLVAEVDGNLVGHVAFSPVTIAEKFLDWYGLGPISVVPGLQKQGIGSKLILDG
                     LARLRDLGAKGCVLEGSPEYYSRFGFKPKVGLTYHAAPAPELFMALPFYDEVPTGQVE
                     YHKAFYISA"
     gene            8945..9910
                     /locus_tag="PLG52_01320"
     CDS             8945..9910
                     /locus_tag="PLG52_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNB40106.1"
                     /translation="MSTVFDSTKTVKNELGKQNYIASDEIATIVYLAQKLGKPLLAEG
                     PAGVGKTELAKAIASATGRELIRLQCYEGLDESKALYEWEYSKQLLYTQLLRDKLNDT
                     LGKAESLSEAADKLAKEEDVFFSTRFLLQRPLLKAILSETPTVLLIDEIDRADAEFEA
                     FLLEILSDFQVSVPELGTLAAKHKPVVVLTSNNTRELSEALKRRCLYLFVDYPSLQEE
                     LAVVHLKVPNLSPKLAQQAVEFVQRLRKADMRKSPSISETLDWANALVALNATNLDKD
                     VLEDTLSVLLKHEADLQKAKRQLMSPPQNPKQRPPADDFGRFSGN"
     gene            9954..>10258
                     /locus_tag="PLG52_01325"
     CDS             9954..>10258
                     /locus_tag="PLG52_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40107.1"
                     /translation="MESRILQLISALRASGVRVSLAESAEAFSAVDLMGIQDRESFRL
                     SLRATLIKDVKDLPTFDKLFPLFFGSGTPPEMGGNPSDDLSPEEIQMLAEALKNFAE"
BASE COUNT         2759 a         2430 c         2497 g         2572 t
ORIGIN      
        1 gttgcagact tgcttgcaga aggtgaacgg atgcccgtct atgtacatgt cattgaccat
       61 ccaaatgggc gtgtgttagt ggatacaggg ttcaccgagt tgcacccagc gatagctgac
      121 cttgacccgc gcatctttcc cttgaacgag cagaatgttg accttgccag cattgatatc
      181 gtcgtcaata cacatttgca cttcgatcat tgcggtggca atcacctctt ccccggtaaa
      241 ccgatctacg ttcagcaagc agagttggat gatgctctca ccaaagacga ctacaccatt
      301 cgcgagtggg ttgatgcgcc tggggtgaaa tatatgccgg tcgatggtga gtttgaactg
      361 cttcccgggt tgaggcttgt ccctgcaccg ggtcataccc gtgggatgca aatggtcgtc
      421 gttgagacag gtgaacgccc aatcatcgtc ggcggtgacg tggcagtttg gttcggcgag
      481 cttgatgagc cgaaaactga aggtcagcta aaggtgcata cacttaaccc gcagttggtt
      541 tggctcacac acgagcatga gccgtggcga ccgaagtaat taatcaaact tgatatattc
      601 aaagaactct atcaagaggt aaaatagccg tcattaatat ggcgtattcc atcaaatatc
      661 cccgcaaggt atttattcga agtacgttcc gttttaccat tcgctcgctt ttccccttat
      721 tagcacaaac aaaaattaca gggtcaaaaa acttcccaca aaacggacct cttattctgg
      781 ttggaaatca cactggcgca atggaagtcg ttctcatggg gttgtattct cccaagcccg
      841 tagagttcat gggtgcaatg gaaatgccct ggaatggttg gatgggaaaa atgatcgacc
      901 tatatggatt gatccctgtc caccgtgggt ataccagcaa cacaactatg aaaatgggaa
      961 ttgatgtttt gcgtcagggt ggaatattgg gaatttttcc cgagggcggt ttttgggagc
     1021 ccggtaagca aaaggcacaa tcaggggtag catggttaag ttatgcggca caagcgccaa
     1081 ttctccccat tggctttggc gatacgcgcg gcaaaatggc agaaatattt cagctaaaac
     1141 gccctgcctt tgagatgaat attggagatg taattccgcc tgtgcaattg gaaaaatctg
     1201 cacacaaaaa agaggaattg caacaagccg cagatcgtat tatggatgca gtttgggcgt
     1261 tggtaccaga agacgagcgc aagcgcaaag aaagtcaacc tgaaaacgaa ttatttactt
     1321 ttgatatatc catctttgac caacatggaa cgcccatacc gattccagca gatttacagc
     1381 taacagacgg ctcatggatc agtcgcttcg cacaccgccc gaacttgatc gattcgatcc
     1441 gagactatat cttcatcccg gtacaggttc taaaagaact acaccgaaag cctcctgcag
     1501 aagaaattta cttggcagca cattctatgc ttgaatatgt agagcgcgat aaccctcaat
     1561 actttaatta tcgctatggg tacaaagacg gcgaagtctt tcagcaaagt tttcgtcaac
     1621 tacgcgacct gatgaaatgg gcaatggaga atcaatatca ggtggcagca gaagcccggt
     1681 atgagtatac agacccaagt acgggcgaga ggcgggtttt acacgtccca caagaattgg
     1741 aacaatggta gatcaaaaat actttgcccg ccatctaaag gagcagacat tccatgagca
     1801 attctgagcg tttatccact cgtttcaaaa tgttattcag taccggggat ctaagcacaa
     1861 gtattccgct tgcgatcttg atgttctttc aactttattt tctaaccgat gttgcccgcc
     1921 tgcgaccgga ctatgccggg tgggcaattg ctctgccgcg tttatgggac gccatcaacg
     1981 acccactttt tgggttgctc tcagatcggt tgcgcactcg ttggggacgc cgccgtgcct
     2041 tattactttt tgggtctgta ccactcggtt taagttttat cttgatgtgg atcgtgccgc
     2101 ccttcggtca acttggattg gcgatttatt atgcgttgac ctttattatc tttgatacgg
     2161 catttactgt cgtccatgtt ggctacaact cgctcacacc tgagctaact tctgattacg
     2221 acgagcgtag ttcattgaac ggctatcgca tggttttctc gatcagtggc acattgggtg
     2281 ctattatcct tgcaaccgtt ttaggttggt tcattaccga tgcgcgtttg ttattcatca
     2341 ttctcggcgt agggctgggt ttggcttcga tcattccgcc tttggttgtt ttcaacatca
     2401 ctcacgaaaa gccagccgat gaacagcctg agccgctttc atggtggcag tcgatgcaat
     2461 tgaccttgag caatcaaccg tttcgaatgg tcatggggtt atatcttctc tcatggacaa
     2521 ccgccagcat tctcgccgcc atcttgattt actttgcaaa ctatcgttta cgtgtgccag
     2581 agcaagcaaa ttattttgta ttgttggctg aaggtgccgc aatctttttc attccgttct
     2641 gggtgtgggt tgccaaaaag ttggataagc gccgtgcctt catcattggc acattgactt
     2701 ggatattcgt cctgcttggc atctcagctc tgcctgccga tgccattggg cttgcatatg
     2761 tgctcgcggc tctttccgga tctggcattg ccgctgcata tgttttgcct tggtcaatga
     2821 tcccagacat tatcgagcac gaccaacttc aaacaggtca acgccgtgaa gggtcttact
     2881 atgccttcgc ttctttcttc caaaaactcg ccacagcttt tgccttatgg gcaatgggac
     2941 aagccctcgc tgcaaacgga tacattaccc caaattcaag cggacctctg ccaacccaac
     3001 ctgattctgc cattcaagcc atccgcttct ttgccggacc cgctcccgcc gtcttgctca
     3061 ttttggcagt tctcttcgcc tggaactatc ccatcacccg cgaaagccac agcgaaacat
     3121 taagagtcct cgccgaacgc gaatcatagg gaggcagtgt gaccgaaaag catacaagct
     3181 gtagcatttg tagcaatttg aaagagtatc aagttgctac agagtacatc gttagcccag
     3241 aagagaacat aaattttcct gagaacgtgt tgactgctat tggaaagtta aaaaaagtaa
     3301 aagaaatctc atccagcaca gatattttcc aatgccctga atgtggcacc tattattttt
     3361 accgtgcaag ttatgaatac ctagtcagcg gcagcggctc gtatgatgaa tatgttttga
     3421 cccgcgaaga cgatgagatt gtaaaaaagt atttgcaaga cgattaggag acaccagtat
     3481 gatcatcgca ttcaaaggca agatatttca atggcgtgga ccagcccctt atcttttcgt
     3541 ggctgtccca gaaaaagaaa gtgccaatat caaagcggtc tctaaactgg tgacgtatgg
     3601 ttggggagtg attcccgttg tagcgaaaac agggaagaca gaatggaaaa cttccctctt
     3661 cccgaaagac ggtcgctatc ttgtgcctat taagatggta gttcaaaaag cggaaaagct
     3721 caaagaaggc gatgaaatca aactgcagct tgaattaaat ctttaaatat tatgaacgaa
     3781 gcctttgcct gataaaccga gcaagggcat tatttttcca ttagtacgag tgatacaatt
     3841 atcaaactta ttgttatgca taaccctgaa ataatcgcca acgagaaaga agaattcaaa
     3901 caagcggtgt tgaacgcgcg tccgttcaag ccgctttatg ttaagttcaa ggtcttttac
     3961 ggctgcaacc tcaaatgtga gatgtgtaat cactggcgcg agacccgcga gccgcctgtt
     4021 acgcctgaac gtttgaaaga agtcatcaac gagttggcag agatgggcac gaagaaaatt
     4081 cacatctctg gcggcgaacc catgctgcga ccgcagattc ccgattttgt ggagttggct
     4141 tcttcgcttg gcatcaaagt gaccatgacc accaatggca cgctcattac caaagagaaa
     4201 gccaaacggc tggtggaagc aggtttacgc ggcgtgaatc tctccattga ctcgcccata
     4261 cgcaaaatgc acgaaaaaat tcgtggcgtg gaagggtcat tcaagttaac aaccaaggcc
     4321 gttgcgcttt tcaaccgcta caagcacaaa ggcaagctga ccattcgcat caataccgtg
     4381 gtcagccgca caaattacga gacgcttgcc acactcccag accttgctca caaactcggt
     4441 gccgatggca ttaacttaat tccggtcgat gaccattgtg gtgagatttt atccatgcgc
     4501 aagaaagaca tcgagaaatt caacaacgag attgcgccac gcatcgccga acgcgctctt
     4561 gaattaggtc tgatcgtttc ggatgaagat gcttttccct ttgggcgaga cgagtccgag
     4621 gtccgcttgg gacgcgctgg acggtatgca tttggcttct atgaaaagca tccctgctat
     4681 gcaccatggt cacacacact gatcgatttc aatggcaatg tctatgtgtg ttgtatgaca
     4741 cgcgaaaaaa tccctccatt gggaaacatc atccatcaat catttagaga aatttgggaa
     4801 ggcgccgcct atcgccaggt gcgcttgaaa atgcatccgc ctgcgctgaa accttgcgct
     4861 cgttgcgatg attttattga ccagaataaa agtatctggg aaacgatcgg tccttactaa
     4921 ctctggttga gaatctgctc aatatagtga agtgtcaact ttgagttcat aataaaggta
     4981 tgaatgctcg gcacttggat caactcataa tcatcaccca ttgaagcatc ttgcacgctc
     5041 aatgccccat cattgggctc gaagccataa ggtagccaac caacacgtgg tccggcagag
     5101 ccggcgatga ccgttgcagg gatattcggt aatggcaatt tttgatagaa gttcgagttt
     5161 agaactaatt gtccgcaatc tgtagtgaga tacctgtaga gaaaattatt ttgaacataa
     5221 ggtgccaact taggcggacg gttcggcgaa gccagtaaaa acaaatgaac tggcaggttc
     5281 ttgagcaaag gcaaaccggc tcgggcaatc aacccgccca tcgaatgccc cacgatggca
     5341 tagggttgcc catcattcac agtgttgacc cactgcacaa aacggtttac gatgccttca
     5401 aatgtttcgc tacgggtgta atatcgaaag tattgcacag gatgcccaag cctgccaagc
     5461 cgaaagccaa gcagccgcat ggcattggtc gtccgccctt gcccgtgaag taaaagaacg
     5521 ttcacaaacg ttagctcaag tctgagaaca tgtcgctgtt atcatcatta tcgtcgaagg
     5581 aactggtgga gccaaacaat accatatccg ccggagtaaa ggtcgaagcg gcaatcggta
     5641 aagcaaccgg catctcaaga ccttgctggc aagcaagaca aacacgccct tcttttgttg
     5701 cgttcgtatg ctcgtcacaa aacccgcgtc cacacttaac gcacgtcccc aatgctgggt
     5761 tatcacaacg atgcggaacc aaataaccaa caaccatttc acactttgcc atcgagacac
     5821 ctcatgaaaa ttgctttgct tagcgaaaag tatacaccag accttggcgg gctcgcaatc
     5881 tcaacaggac gattggggga gatgcttgcc gcggctggac atgacatccg cctcttcgcc
     5941 ccgacttcaa acctgtcacc caccatcaag cagactcaac gctcaagcgg agtcaatgtc
     6001 actcgcttcg gcgcacacaa acgcgtggac aatacattag tggattggtt cgagttgatc
     6061 gtggaagaac ataagcgcga gccgttcgat ctgattcatg cctacttcct ccccatggcg
     6121 gggttcgtcg gcacctatgc cgggaaatat ctaggcattc ccagcgtcgt ctccattcgt
     6181 ggaaatgaca ttgaacgcgc cgccttcgac ccaagcaaat tttcacatat gatgtatgcc
     6241 ttacaaaatg caagtgctgt aacaaccaac gccagcattt tggcaaagaa agcaaaagcc
     6301 ttttttgacc gtgaggcaca tatcattccg aatggaattg atacagaacg gtttaaaccg
     6361 atggaaaaaa attcagtctt agccgaagca ctgggtttgg tggatgaaaa gaagaaagaa
     6421 gaaggaaaat ttgtcatggg gtttgttggg gaattaagag aaaagaaggg acttgcaacc
     6481 ctcctttctg gctacgcgca agctacaaaa accagaccgg tttctctgct tattgttggc
     6541 gaagtacgag aaggcgaaga taaaaagtat tttgaagaat tcaaaagcaa caatccgcaa
     6601 ctaagcatta ctgttactgg acatgtcccc cacaaagact tacctgcata ttattccttg
     6661 atggatgtct ttgtacatcc gtcgctgcgg gatgggatgc cgaatgccgt tctcgaagcc
     6721 atggcatgcg gagttccagt cattgcaaca ccagttggtg gagcgttaga tgtgttggaa
     6781 gatggcgtga atggatttct cgtagatgta aatgatgcac cacaactagc agagaaaata
     6841 gctaaaacgc taaaaccgct taaagaatta gaatcagttc gtagatccgc acgagaaacg
     6901 gttctacatc actgtacgct tgagaaagag ttgcaagcaa atctaaaaat ttatgcaaac
     6961 cttggagtca caacaaaatg aagcatcgac ttgagcacga tcttttgggc gaacgtgaga
     7021 tcccgaatga ggtgtattac ggggtgcata cgttgcgtgc gctggaaaat ttcccgatca
     7081 gtaaaattcc gatcagcaat tatccaagtc ttgtaaatgc attggcatgt gtcaaggagg
     7141 cgtgtgcaaa agcgaatttg gaattgcaat tgctagagcc cgaaaaagcc aatgccgtca
     7201 ttaaagcttg tgaagacgtg cgtggcggag cactacataa tcagttcgta gttgatgtta
     7261 tccaaggtgg cgccggtact tctacgaaca tgaatgcgaa tgaagtgatt gccaatcgcg
     7321 ggttagagat tcttgggcat ggacgtgggg aatataaatt cttgcatccg cttgaagatg
     7381 tgaatatgag tcaaagtaca aatgacgttt acccaactgc tgtcaaagta gcgttacggt
     7441 ttttgatcaa tgacttaatt gacggcatgg aagtattacg tctcgcattt gcggcaaaag
     7501 ccgaagaatt caaagatgtt ctaaaaatgg gacgcaccca gttacaagat gcggttccca
     7561 tgacgctcgg gcaagaattc agcacgtatg ctgtgatgct tgaagaagac caacagcgcc
     7621 tccgtgaagg ggcattgctc attcaagaga tgaacctcgg cgcgactgcc attggcacgg
     7681 gcattaatgc acaccctgaa tatgctgccc tcgctcgcaa atatctaagt gaaatcacag
     7741 gaattgaata catcactgct ggaaatctcg tggaagctac ccaagatgtt ggctcgttcg
     7801 tacaactctc aggtgtgctt aagcgtgtgg cagtaaaact ctccaagatc tgcaatgact
     7861 tgcgattgct ctcctccggt ccgcggacgg ggctgggcga aatcaacctc cctgcagtgc
     7921 aagccggttc gagcatcatg ccgggcaaag tcaatccggt cattccggaa gtggtgaacc
     7981 agattgcttt tctcgtcatc ggcaatgacg tcactgtctc tttcgcagcc gatggcggac
     8041 aattacaact caatgccttc gagccgatta tcgcacacag tctatttgat agtctcattt
     8101 acctgcgcaa tggctgtatt actttagctg agcgctgtgt aaaagggatc accgccaacc
     8161 ataaccgtct tgaagaacat atgaaccgct cggttggaat cgttacagcg ttgaatccgt
     8221 tcatcgggta cgaaaatgca agctcggtgg cgaaagaggc gtacacatcc aacaagagcg
     8281 ttaccgaaat cgtactggag cgcggtctgc tcacaaaggc agagcttgat gaaatcctca
     8341 aaccggaagt gctcaccaag ccgcgttgga ttaagaaaga atctggacaa aagtaagtat
     8401 gggcgttctc gttcggtctg aaacagcaag tgatattgaa gccatttcac aagtcacgta
     8461 cgctgcgttt cttggcaagt tcagcgacca tcccacggaa catctcatag tgaatggttt
     8521 gcgtaatgca ggcatgctca ccctttcact cgtggctgaa gtggacggaa accttgttgg
     8581 gcatgtagcg ttctcacctg tgaccatcgc tgaaaagttt ttggattggt atgggttggg
     8641 tccgatctca gttgtgcctg gtttgcaaaa acaaggcatc ggctcaaaat tgattttgga
     8701 tggattggca agattgcgag atctcggagc gaaaggatgc gtgctcgaag gcagcccgga
     8761 atattactcg cgcttcgggt tcaaaccgaa agttggtctt acttatcatg ccgcacctgc
     8821 cccggagctt tttatggcac ttccttttta cgatgaagtt ccaactgggc aagtggagta
     8881 tcacaaagcg ttttatataa gtgcatgacg atttactacg tttcgctgtc gaataaggag
     8941 agagatgagt actgtttttg attcaacgaa aaccgtaaag aacgaacttg gcaaacagaa
     9001 ttacattgcc tctgatgaaa ttgcaaccat cgtatatctt gctcagaagt taggcaagcc
     9061 attgctagcg gagggtccgg ccggcgtagg caagacggag ttggcaaaag cgattgcttc
     9121 ggcaacggga cgtgaactca tccgcttgca atgctatgaa ggcttggacg agtccaaagc
     9181 gttgtatgag tgggaatatt ccaagcaatt gttgtacacg cagttattac gcgataaatt
     9241 gaacgataca ttgggcaaag ccgaatcact gagcgaagcc gccgataaac tggcaaagga
     9301 agaagatgtc tttttctcga cgcgcttttt gctgcaacgt ccgctgttga aagcgatctt
     9361 gagcgagaca cccacagttc tgttgatcga cgagatcgac cgcgctgacg cagagttcga
     9421 agcctttctg ctcgaaatct tgagcgactt ccaagtctct gtgccagagt taggcacact
     9481 cgccgcaaaa cacaaacctg ttgtagtgtt gacttccaac aacacccgcg aactctccga
     9541 ggcgttgaag cgccgctgtt tatatctgtt cgtggattac cccagtttac aagaggagtt
     9601 ggcggttgtg catttgaagg tgccgaatct aagccccaaa cttgcccaac aagccgttga
     9661 attcgtgcag agactgcgca aagcagacat gcgcaagtca ccatccatta gtgagactct
     9721 cgattgggca aatgcgctcg tagcattgaa cgcaacgaat ttggataaag atgttttgga
     9781 agatacactt tctgttctat tgaagcacga agcggatttg caaaaagcaa agcgacagtt
     9841 gatgagtccg ccgcagaacc cgaagcaacg tccgcccgcg gatgattttg gtcgcttctc
     9901 aggaaattga tttgcatggg cggggtttcc ccgctactat gggataaact tttatggaat
     9961 ctcgcattct ccaattgatc tcagccctgc gcgccagcgg ggtgcgcgtg tcgctcgcgg
    10021 agtctgccga ggcgttctcg gctgtggact tgatgggcat tcaagaccgt gagtcgtttc
    10081 ggctgtcttt gcgcgctaca ctcatcaagg atgtgaagga cctccccacc ttcgacaagc
    10141 tcttcccgct cttcttcggc tccgggactc cgccggagat gggcggcaac ccatcggatg
    10201 atctgtcgcc ggaagagatc cagatgctgg cagaggcgtt gaaaaatttc gccgagca
//
LOCUS       DAOQNJ010000022        10121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3129_length_10121_cov_7.503775, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000022 DAOQNJ010000000
VERSION     DAOQNJ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3129_length_10121_cov_7.503775"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLG52_01330"
     CDS             <1..955
                     /locus_tag="PLG52_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40108.1"
                     /translation="VFACNLPSASTPTPDLFATLSAVTPAPADSTSQPNPSTSSTAEP
                     TGKIVFVCQIFKTQASNQICIINADGSGFRRLTTDNTRQHYYPSLSPDGKSVVYAAFR
                     EPNIYEIYEMDIATGNVAQLTDRLGNLNGPEISPDGSMIAFKLSTAAANQIWLMNRDG
                     SDPHPIPNAFGWDPTWSPYGTSILFASDMQGVVQLYVIKLDGSELQKISDLPAIRGRS
                     DWSPNGQFIVTYSGQPWNRDVYIMNADGSDARMLTPTGGNSQGPSISPDGQWVVFTSY
                     YDHYGEDHGCEIYIMPVNDTQQVVRRLTDNDYCDYQPRWGP"
     gene            972..1799
                     /locus_tag="PLG52_01335"
     CDS             972..1799
                     /locus_tag="PLG52_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB40109.1"
                     /translation="MDIKGKVVIITGASSGIGAAAALEFGREGAKVVLAARRTDKLQE
                     LVNEIRAMQNGAEAFVLQTDISKLDDIQRLVAQTVERFGRVDVLVNNAGFGRLKWLET
                     LDPLEDIQAQFDVNVLGVIQTTRQVLPIMIKQRSGSIINICSIAGLVGTPTYTIYSAT
                     KHAVHGFSEALRREVKPWGIDVSLIYPGGVRGTEFPDHAGIQRKTNASTPKFMVLTAE
                     QVGKAVVNLVHRPRRMWVLPWMWRFTIFLNRFLPGLVDRTTIKNFTIPERADELNKK"
     gene            complement(1823..>3273)
                     /gene="recJ"
                     /locus_tag="PLG52_01340"
     CDS             complement(1823..>3273)
                     /gene="recJ"
                     /locus_tag="PLG52_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNB40110.1"
                     /translation="DGVTATALLVETLKKLGADVRGYIPNRFDEGYGLNNNALDELKA
                     DGVKLVITVDCGIRSPAEAAHAQTIGLDLIISDHHHPDDDNLPPALAVINPKRHGDIY
                     PDKDLAGVGIAYKIAEALLTKTDQADVVNGQILNSLLDLVALGTVADLAPLVGENRVL
                     VKKGLKQMRQTTRQGLFSLAAVADISLAKVNAGNIGFGLGPRLNAAGRLKEALAAFEL
                     LTTTDVFRAGELAQQLDMQNRERQRITRDMQTRAEEIALNDDPNAHLLFAAHEEFNSG
                     VVGLAASRLTEKYYRPAIVASKGEEETRGSCRSIPEFHITDALDQCADLLVRHGGHAA
                     AAGFTVRNENIEALVSKLKAIAEEKLAGSDLKPTVTADAEVDLSEMRPEVYEKGLKYL
                     EPTGYGNREASFVARNVKVKSSRIVGSDGKHLKMSLEDSKGIAHDAIGFRLGDWHKSM
                     PPRVDVLFTYEPNEYNGRVTYQLNLKDLKASR"
     assembly_gap    3274..3373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3374..3690)
                     /locus_tag="PLG52_01345"
     CDS             complement(<3374..3690)
                     /locus_tag="PLG52_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40111.1"
                     /translation="MTQDKLWNISPRISPEADSALAAFPPLLRQVLFNRGFADDPSAR
                     AYLRGEPNSNTNPFQITDMQVAIERIRYALANNEPIAIYGDYDVDGVTATALLVETLK
                     KLGA"
     gene            3766..4281
                     /locus_tag="PLG52_01350"
     CDS             3766..4281
                     /locus_tag="PLG52_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40112.1"
                     /translation="MRALLPDEKLSTVFIYTPTMLVRGDLILRENIRASIWLRTQGVP
                     NYIHLYAAQVIQLAGSPPKNYTSQETFIPTAEVIGFHLAPPAQDPLDYDATEANRKME
                     SVHVLAGSFEMKAKLRISTSADFASGLDVMNTSWLSLYEAEITNPYLPQLKLTVPMLL
                     ARPSKLTISLL"
     gene            4314..4718
                     /locus_tag="PLG52_01355"
     CDS             4314..4718
                     /locus_tag="PLG52_01355"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNB40113.1"
                     /translation="MEELTLTTPALLFPTVSLLMLAYTNRFLTLATIIRNLNDRYHDS
                     QDKNLLAQIDNLRYRVYLIRNMQIFGVLSLLMCVVSMFALFFNWTDGGVWSFGIALVL
                     MIISLGISLRELQISVGALDLLLTNLEEHETK"
     assembly_gap    4720..4819
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4901..5737
                     /locus_tag="PLG52_01360"
     CDS             4901..5737
                     /locus_tag="PLG52_01360"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNB40114.1"
                     /translation="MDTDKLIQTMVDEIKTVSGVKAIVLGGSRARGTYTPSSDIDLGI
                     YYDPNQPMDLTALGKIATQLDDEHRTDVITGFGGWGPWINGGGWLRVQSLPVDFLYKD
                     LGRVKSYIDACLNGKVEIFYQGGHPHGFVTSIYLGEVALCKPLYDPEGIIAELKGKVT
                     PYPEALQKAILASFAWEIDFSVGIAKKSIERADVNYAAGCCFRAVMCMLQVLFALNKE
                     YWMNEKGAINITDRFAIKPADFRKRVAEVYALLEASPEALEKAIYLLKGLSEDVYGLL
                     PK"
     gene            complement(5795..7222)
                     /locus_tag="PLG52_01365"
     CDS             complement(5795..7222)
                     /locus_tag="PLG52_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNB40115.1"
                     /translation="MKTLLIPLDTNATKALYLQVADYITEAARSGQLKPGTQLPPSRT
                     LAEQNNVHRSTIINAYQELKARGVLASKQGSGTYIEKGLSENTVSIQKFKPESLPTDE
                     LIAEIRRMHWSEGMISLGYGPPSDELMPVEDFDRSRQRVLRRDGKKTANYEHPQGYYP
                     LRQAICADLVKQGIQADPDEILITNGVLEGVALVSRAMAAPGDAVLTELPQYFGNWTN
                     FSYLGLNVRGFDLLESGPDFASLEAQLQDENDRPRFVFVTADHQNPLGTCWTMPARHE
                     FLKMMNKYDVPVVEDATYRDLTYDGMLHTPLRALDPDVIYVGTFSHSLMPGLRIGFVL
                     CRGRLREHIERLKAISSGPVETLNQRSLADFLATGEYERHLDRIIPVYRARRDALLNA
                     MQTYFPPELRWTKPTGGFFVWIWLHDHISAIDLFYRSVKKGISIAPANIFYPGPCPQN
                     AFRLAFSHYPEDVLTRAVMEIGKLL"
     gene            complement(7219..7842)
                     /locus_tag="PLG52_01370"
     CDS             complement(7219..7842)
                     /locus_tag="PLG52_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40116.1"
                     /translation="MNIKKTLPVVLIAGFLLIVACMSSPTPTELPIPTPMTVELTQPP
                     HLDPKYFLTATLVPFVTLAPTSTFEGTPAIVNIALNMGAGGAGFSANLYAQEVYTGRT
                     VGIFMSAGMHTAPLLPTSPPLDMAVPAPGTYVFYARLSNDPMDYHYGYTQCQVPTDCV
                     GSPLIAMKVEPGQRYNILIADRKALLPEINERGLPVTVPYVLVESNP"
     gene            complement(7859..8404)
                     /locus_tag="PLG52_01375"
     CDS             complement(7859..8404)
                     /locus_tag="PLG52_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40117.1"
                     /translation="MTKPRLTHSLFLILLLTACAAPATAAPTQTSTPKAPEATSTIDN
                     STPTLTVTPDAKLATVEGTMTWLPSGTDEILPVPDVTMQLDRHDGEYLKYKVRTEANG
                     HYVFANIEPGTYGVGMYLNLRVGERQCGAPEFTSTLDLGWEHYSTWSKVDVWYDVIFS
                     DTDITIKPGEAITIDYQLKCP"
     gene            8534..9661
                     /gene="tgt"
                     /locus_tag="PLG52_01380"
     CDS             8534..9661
                     /gene="tgt"
                     /locus_tag="PLG52_01380"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNB40118.1"
                     /translation="MFDFSITAKNNRARTGVFSTPHGDLITPVFAPVGTQATVKTLTP
                     EHLKSINASLVLSNTYHLYLRPGDELVRDMGGLHKFMQWHNPMLTDSGGFQVFSLSQT
                     RKVDEDGVTFKSHIDGSTHRFTPEKSIAIQENLGADIIMAFDECSDPNDHAYIKKAME
                     RTHRWAERSLKAKTRSDQALFGIVQGGVDPTLRAESAKFIASLETPGIAIGGLSVGET
                     KQEMHDTLDVVTPLLPENKPRYLMGVGTPEDLINGVLRGVDIFDCVLPTRLARHHSAF
                     APEGRLNMMNASFARDERPIDETCDCYACQTFTRAYIRHLIVAKEMLASTLVSIHNLR
                     ALIRLMENIRVYIAEGSFEDRAPELLLQWGNNAKRVVNGVK"
     gene            9662..>10121
                     /locus_tag="PLG52_01385"
     CDS             9662..>10121
                     /locus_tag="PLG52_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNB40119.1"
                     /translation="MNIFHAIVLGIVQGLTEFIPVSSTAHLFIVSNLLGLPSDDQVFA
                     FDVIIQLGTVGALLLFFWHDYWDIAKAVFEGIKNRKPFEDYHSRLGWLVIVATIPAGV
                     VGLALKPLVQQMSGDPLLWGGIRLLFTSALLAAVEYFDKKSRTLESATWID"
BASE COUNT         2391 a         2265 c         2613 g         2652 t
ORIGIN      
        1 cgtctttgct tgcaatttac cctccgcttc taccccaacc ccagacctgt ttgccactct
       61 atcagcagtt acccccgccc cagcagactc tacctctcag cctaacccat caacctcatc
      121 cacagcggag ccaactggca agatcgtttt cgtttgtcag atattcaaaa cacaagccag
      181 taatcagatt tgcatcatca atgccgatgg ctctggtttt cgccgtttga ccaccgataa
      241 cactcgtcaa cattattacc catcactatc gccagatggc aaaagtgttg tttatgcagc
      301 atttcgtgaa ccgaatattt acgaaatcta cgaaatggat attgccactg gcaacgttgc
      361 tcaactaacc gaccgtctcg gcaatctcaa cggaccggaa atttcgcctg atggttcaat
      421 gatcgcgttt aaactctcca ccgcggcagc caatcagatt tggttgatga accgtgatgg
      481 gagcgacccg catcccattc ccaatgcatt tggctgggac ccaacttggt ctccatatgg
      541 aacctccatt ttgtttgcct ctgatatgca aggtgtcgtt cagctctatg tgataaagct
      601 cgatggaagc gaactgcaaa agatcagcga cctgcccgcg atacggggaa gaagcgattg
      661 gtcaccgaat ggacagttca tcgtcacata ttcggggcag ccttggaatc gcgatgttta
      721 catcatgaat gcagatggtt cggatgcccg catgctcaca ccaacgggcg gtaactcgca
      781 aggtccatcc atttcaccag atgggcagtg ggtggtgttt acctcttatt atgaccatta
      841 tggcgaggac catggatgtg agatttacat catgcctgtg aatgacacac agcaagttgt
      901 acgccgacta acagacaacg attattgtga ttaccaaccc cgctggggac cttaagaaca
      961 acaaggacaa catggacatc aaaggaaaag tagtaatcat caccggcgct tcgtcgggga
     1021 ttggggcagc ggctgcgctt gaatttgggc gtgaaggggc gaaggttgtg ttagctgcac
     1081 ggcgaactga caagttacaa gaattggtga atgagattcg tgcaatgcag aatggcgcag
     1141 aagcctttgt gcttcaaacc gatatctcaa aactggacga cattcaacgt ttggttgctc
     1201 aaaccgtgga gagatttggt cgtgtggatg tgttggtgaa taatgcaggc tttggacgtt
     1261 tgaaatggct cgaaacgctc gacccacttg aagatattca agcacaattt gacgtgaatg
     1321 ttttgggcgt tattcaaacc acacgacaag ttttgccgat catgatcaaa cagcgcagtg
     1381 ggagtattat caacatctgc tccattgctg gtttggtggg cacgccgacc tatacaattt
     1441 actctgctac caaacatgcc gtgcatggct tttcagaagc tttgcggcgc gaagtcaaac
     1501 cttggggaat tgacgtctct ctcatctatc ccggcggcgt gcgtggaaca gaattccccg
     1561 accatgccgg tattcagcgc aagaccaacg cttccacgcc aaagttcatg gtattaacgg
     1621 cggaacaagt aggcaaagct gtggtgaatc ttgtgcaccg tccgcgccgt atgtgggtat
     1681 tgccttggat gtggcgtttt actatttttc tcaatcgttt tttgccaggg ctagtggacc
     1741 gtactacgat caaaaacttc actattccag agcgtgccga tgaactgaat aaaaaataga
     1801 cagccgacag gctgtctatt ttttatcttg aagctttcaa gtctttcaaa ttcaattgat
     1861 aagtgacgcg cccgttgtat tcatttggct catacgtgaa aagcacatcc acccgtggcg
     1921 gcatggactt gtgccaatcg ccaaggcgaa agccgatcgc atcgtgagca atgcctttgg
     1981 aatcttccaa tgacatcttc aaatgcttgc catcagaacc cacaatgcgc gaacttttga
     2041 ctttgacatt gcgtgccaca aacgacgctt cacgattgcc atacccggtt ggttcgagat
     2101 acttcaagcc tttctcgtac acttctggac gcatttcgga taagtccact tccgcatctg
     2161 ccgtaacggt gggcttgagg tcacttccag cgagcttttc ttcggcgata gcttttagct
     2221 ttgagacaag tgcttcgata ttttcatttc taactgtaaa ccctgccgcc gcggcatgac
     2281 cgccatgccg cacgagcaag tctgcgcatt gatcgagcgc atcggtgatg tgaaattctg
     2341 ggatggaacg gcaggagcca cgcgtttctt cttcgccttt cgaagcgaca atggcgggac
     2401 ggtaatattt ttcggtgagg cgtgaggcag cgagacctac aacccctgag ttgaactctt
     2461 catgagcggc aaagagtaga tgtgcatttg ggtcatcatt caatgcgatc tcttcggcac
     2521 gggtttgcat atcgcgcgta atgcgttgac gttcacggtt ttgcatgtct aattgctggg
     2581 cgagttcgcc ggcgcggaag acatctgttg tggtgagtaa ctcaaacgcc gctaacgcct
     2641 cttttaaacg acccgccgcg ttcaagcgtg gaccaagccc aaagccgata ttcccggcat
     2701 ttactttcgc taacgaaata tctgccacag ccgccaatga gaacaaaccc tggcgagtgg
     2761 tctggcgcat ttgcttaagt ccttttttga cgagcacgcg attttcaccg accaggggag
     2821 cgaggtctgc gacggtgccg agagcaacga gatcgagcaa ggagttgaga atttgcccat
     2881 tgaccacatc cgcttggtct gtcttggtga gcaaggcttc tgcgatcttg tatgcaatgc
     2941 ctacgccggc gaggtcttta tcgggatata tatcgccatg acgtttcggg ttgatgacgg
     3001 caagcgcggg cggcaggtta tcgtcgtcgg ggtggtggtg gtcgctgata atgagatcca
     3061 gcccaatggt ctgcgcatga gccgcttcgg cgggcgaacg gatgccgcag tctactgtga
     3121 tgaccagctt gaccccatcc gcttttaact catccaatgc attgttgttg agcccatagc
     3181 cttcatcaaa gcggttggga atatatccgc gcacatccgc accgagtttt ttgagcgttt
     3241 ccaccaataa tgcggtagca gtgactccgt caannnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnngcaccga gttttttgag cgtttccacc aataatgcgg tagcagtgac
     3421 tccgtcaaca tcgtagtcac cataaatggc aatgggttcg ttatttgcaa gcgcatagcg
     3481 gatgcgctct attgcgactt gcatatcggt gatttgaaaa ggattggtat tgctgttggg
     3541 ttcgccgcgc aaataggcac gtgcgctggg gtcatcggca aagccgcgat tgaaaagcac
     3601 ctgacgtaac agcggaggaa aggctgccaa agctgaatca gcttcggggg agatgcgagg
     3661 cgagatgttc caaagtttgt cttgtgtcat gttaaagtgt gcggagtata aacgagacca
     3721 aagtcaagag gaagggcgcg agtataatct gttgaggagc gacgcatgag agctttattg
     3781 cctgatgaaa aattatcgac tgtgtttatt tatacgccta ccatgttggt gcgtggagat
     3841 ttgatcttgc gagaaaatat ccgcgcaagt atttggttac gcacgcaggg tgtgccaaat
     3901 tacattcact tgtatgcggc gcaggtgatt caattggcag gttccccgcc caagaattac
     3961 accagccagg aaacttttat tcctactgcg gaggtgatcg gttttcatct tgcgcctcct
     4021 gcgcaagacc ctttggatta tgacgcgacg gaagcaaatc gtaaaatgga gtctgtgcac
     4081 gtgttggctg gttcttttga aatgaaagcc aagttgcgca tctctacctc cgccgatttt
     4141 gcctccggtt tggatgtgat gaatacatcc tggctttctc tgtatgaagc agagatcaca
     4201 aatccgtatt tgccgcaatt gaagttgaca gtgcctatgt tgttggcacg ccccagtaaa
     4261 ctgacgatta gtttgttata gaatgtgcgg catatgccgc ttgagtattt actatggaag
     4321 aactgaccct taccacccca gcacttttgt ttcctacggt gtcgctactg atgttggcgt
     4381 acacgaatcg ttttttgacc cttgccacga tcatccgtaa tttgaatgac cgctatcacg
     4441 atagccagga taagaacctt ctagcgcaga tcgacaacct acgttatcga gtgtatctca
     4501 ttcgaaatat gcagatcttt ggtgttttga gcctgttgat gtgtgttgtc agcatgttcg
     4561 ctttgttctt taattggacg gatggtggtg tgtggagttt tggcatcgct ttggtgttga
     4621 tgattatctc gttgggaatt tctttgcgcg aattacaaat ttcagtaggc gctttggatt
     4681 tgctgttgac aaatttggaa gaacacgaga cgaaataaan nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnng taggcgcttt ggatttgctg ttgacaaatt tggaagaaca
     4861 cgagacgaaa taaaaagcgt ctccgaaagt gtaggtggtt atggatacgg ataaacttat
     4921 tcaaactatg gtcgatgaaa tcaaaacggt ttcaggcgta aaagccattg tgttaggcgg
     4981 ctcgcgtgcc agagggacgt ataccccttc ttctgacatt gacttgggaa tttactatga
     5041 cccaaatcag cccatggatt tgaccgcttt gggtaagata gcgactcagc ttgatgacga
     5101 acaccgaacg gacgtcatta ccggcttcgg cggttggggt ccgtggatca atggtggcgg
     5161 gtggttgaga gttcaatcgt tgccggtgga ctttttgtat aaagatttgg ggagagttaa
     5221 atcctatatt gatgcttgtt tgaatggcaa agtggaaatt ttttaccaag gcgggcatcc
     5281 gcatggattt gtgacctcaa tttatttggg ggaagttgcg ctttgcaaac ccttatatga
     5341 cccagaaggt atcattgcag aattgaaggg caaagtcacg ccgtacccgg aggcgttgca
     5401 aaaggcgatt cttgccagtt ttgcttggga gatcgatttt tccgtgggaa ttgccaagaa
     5461 aagcatcgaa cgagcggacg taaattatgc ggcgggttgt tgcttccgtg cagtgatgtg
     5521 catgttgcag gttttgtttg cgttgaacaa agaatactgg atgaacgaaa aaggcgctat
     5581 caacatcaca gaccgttttg ccatcaaacc cgcagatttc cgtaagcgtg ttgcggaagt
     5641 ttatgcgtta ttagaagcct ctcccgaagc gctagagaaa gcaatctatt tgttgaaggg
     5701 tttgagtgag gatgtctacg ggctgttgcc gaagtaaagt tgaatttaaa aagacctcgg
     5761 aggttttaaa aacctccgag gtcttttgtt ttaattacaa caacttccca atctccatta
     5821 ctgcccttgt caacacatct tcagggtaat gagagaatgc caaacgaaat gcattttgcg
     5881 ggcagggacc ggggtagaaa atattcgcag gggcgatgct gattcctttt ttcacggaac
     5941 gatagaacaa gtcaattgca gaaatatggt catgtaacca gatccacacg aagaagccgc
     6001 cggtaggttt cgtccagcgg agttctggcg ggaagtaggt ttgcatcgcg tttagaagcg
     6061 catcgcgccg tgcgcggtag acggggataa tgcgatcaag atgacgttca tattcacccg
     6121 tagcaagaaa atctgccaac gagcgttggt taagcgtttc gacgggtccg ctggagatgg
     6181 ctttgaggcg ttcaatgtgt tcgcgcagac gcccacggca aagcacgaat ccgatacgga
     6241 gaccgggcat cagcgaatga gaaaacgtcc cgacatagat cacgtctggg tccagagccc
     6301 gaagcggtgt gtggagcatg ccatcgtaag tcaagtcgcg ataggtcgca tcctctacga
     6361 caggcacatc gtatttgttc atcatcttaa gaaattcgtg gcgggcgggc atcgtccaac
     6421 aagtgccaag cggattttga tgatcggctg tcacgaagac gaatcttggt ctgtcatttt
     6481 catcttgaag ctgcgcctca agcgacgcaa aatcgggtcc gctttcgagc aggtcaaagc
     6541 cgcgcacatt caaaccgagg tacgaaaaat ttgtccaatt gccaaagtat tgtggcagtt
     6601 cagttaacac ggcatcaccc ggtgccgcca tggcacggct cacaagtgca acgccttcca
     6661 agactccatt tgtgatgagg atttcatctg ggtctgcttg aataccctgc ttgacgaggt
     6721 ctgcgcagat ggcttggcgc agtggataat acccttgcgg atgttcgtag ttggctgtct
     6781 tttttccatc gcgcctcaac acccgctgac gtgaacggtc gaagtcctcc acgggcatca
     6841 gctcatctga cggcggacca tacccaagtg agatcatgcc ttcagaccaa tgcatacggc
     6901 gaatttctgc gatcagttcg tcggtgggca ggctttctgg cttgaacttt tgaatcgaca
     6961 cggtgttctc gctcaatccc ttttcgatgt aagtcccgct tccttgtttg gatgccagca
     7021 caccacgcgc tttgagttcc tgataggcat tgataatagt tgagcggtgg acgttattct
     7081 gctcagccaa ggtgcgcgaa ggcggtaact gtgtgccggg ctttaactgt ccgctgcggg
     7141 cggcttcggt gatgtaatct gcaacttgca ggtaaagcgc tttggtggcg ttcgtatcta
     7201 aagggatgag caatgttttc acgggttgga ttctaccagt acgtatggaa cggtcactgg
     7261 caatccgcgt tcgttgattt caggtagtaa tgctttgcgg tctgcgatga gaatgttgta
     7321 gcgctgaccc ggctccactt tcatggcgat caacgggctg cccacacaat cggttgggac
     7381 ttggcattgg gtatagccgt agtgatagtc catcgggtca ttgctgagac gggcatagaa
     7441 gacgtatgtt cccggcgcgg gtactgccat atccaatgga ggcgaggtgg gtaatagtgg
     7501 cgctgtgtgc atccctgccg acataaagat gccgacagtt ctacccgtat acacttcctg
     7561 tgcatataaa ttagcagaga aacccgcgcc acctgcgccc atgttcaagg caatgttgac
     7621 aatagcaggc gtcccttcga aggtggaggt tggggcgagg gttacaaacg gaacaagagt
     7681 ggcggtgaga aagtatttgg ggtcgaggtg gggcggctga gttaattcca cggtcattgg
     7741 agtgggaatg ggtaactcag ttggcgttgg gctagacatg cacgctacta taagtagaaa
     7801 cccagcaatc aatacaacgg gtaaagtctt tttgatattc atcagtctct cctgaaattt
     7861 acgggcattt caattgatag tctatagtga tggcttcgcc gggcttgatg gtgatatctg
     7921 tatccgaaaa tatgacgtcg taccacacat cgactttgct ccatgttgag tagtgttccc
     7981 accccaaatc taaggtggag gtgaattcgg gggctccaca ttggcgttca ccgacccgca
     8041 aattcaaata catgccgacg ccataagttc ctggctcgat attggcaaag acataatgtc
     8101 cgttcgcttc ggtgcgaact ttgtacttga gatattcacc atcatggcga tccagttgca
     8161 tagtcacatc gggcacaggc aaaatctcat ccgttccaga gggcagccaa gtcatggtcc
     8221 cttctacagt agcaagcttc gcatcggggg tgactgtcaa tgtaggtgtg gagttgtcga
     8281 tggtggaggt tgcttcgggc gctttagggg tggaggtttg tgtgggagcg gcagttgcgg
     8341 gcgcggcaca agctgtcagc aacaggatca aaaataggct atgggtgagg cggggttttg
     8401 tcatgatttc tctctctaag aataagtcta ttattgcaat tatatgaaac ggtctatgcc
     8461 tgaaaaagag ccaattgaga ggcagtttgc ataccaattc ggtgatacac tttcatcctg
     8521 tataatttgc cccatgtttg atttcagcat tacagctaaa aataaccgcg cccgcaccgg
     8581 ggttttctcc acgccgcacg gcgatctcat tactcctgtg tttgcaccag tgggtacaca
     8641 agccactgtc aaaacactga cgcctgaaca tctcaagagc attaacgctt cactcgtctt
     8701 gagcaatacc tatcatttgt atctgcgccc tggcgatgaa ttggtgcgcg atatgggcgg
     8761 gctgcataaa ttcatgcagt ggcataaccc catgcttact gattcgggtg gctttcaggt
     8821 tttttctctt tcgcaaactc gcaaagtgga tgaagatggc gtgaccttca agagtcatat
     8881 cgatgggtct acgcatcgtt ttacgccaga gaagtcgatt gcgattcaag agaatctcgg
     8941 cgccgacatc atcatggcat ttgatgaatg ttctgacccg aacgaccatg catatatcaa
     9001 gaaagccatg gagcggactc atcgttgggc ggagcgttcg ctgaaagcaa agacgcgctc
     9061 agaccaagcc ttgttcggga tcgtccaagg tggagtggat cctacgctgc gagcggagtc
     9121 tgccaagttc atcgcctcgt tagagacgcc tggcattgcg attggtggtt tatctgtcgg
     9181 tgaaaccaaa caagagatgc acgatacgct ggatgtggtc acaccgttgt tgcctgagaa
     9241 taagccgcgc tatctgatgg gcgttggcac acctgaagac ctcatcaacg gtgtcttgcg
     9301 gggcgtggat attttcgatt gtgttttgcc aacccgtttg gcgcgccatc attctgcttt
     9361 tgcaccggaa ggtcgcctta atatgatgaa tgcttccttt gcgcgcgatg aacgccctat
     9421 tgatgaaacc tgtgactgtt atgcttgtca gacttttaca cgggcgtaca ttcgtcactt
     9481 gattgtggca aaggaaatgc ttgccagtac tttggtttcc atccataatt tgcgggcgtt
     9541 aatccgctta atggagaata tccgcgttta tattgcggag ggctcatttg aagatcgtgc
     9601 gccagagttg ttgttgcagt gggggaataa tgcaaagcga gtagttaacg gagttaagta
     9661 attgaatatt tttcatgcaa ttgttttggg gattgtacaa gggttgaccg aattcatccc
     9721 tgtttcttct acggctcact tgtttattgt gagcaattta cttggtcttc cgtcagatga
     9781 ccaagtattt gcatttgatg tgattattca attaggaaca gtgggagcat tgcttctatt
     9841 cttttggcat gattattggg atatagccaa agctgttttt gaaggtatca aaaaccgaaa
     9901 accctttgaa gactatcatt ctcgtttggg gtggttggtg attgtagcaa ctattcctgc
     9961 aggggtggtg gggttggcgc ttaagccgtt ggttcaacaa atgtctggcg acccactgtt
    10021 atggggcgga atacgattgt tattcacttc agcgttattg gcggctgtcg aatattttga
    10081 taaaaaaagt cgcacgcttg aatcggcaac atggatagat g
//
LOCUS       DAOQNJ010000023        10029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3174_length_10029_cov_9.111791, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000023 DAOQNJ010000000
VERSION     DAOQNJ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3174_length_10029_cov_9.111791"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PLG52_01390"
     CDS             <1..860
                     /locus_tag="PLG52_01390"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNB40120.1"
                     /translation="DWSIIDMKHQEFNWKAKDGLDIFAQVWEPDVVLPRAVVCLVHGL
                     GEHSSRYAHVAEAFSKEGYILFSFDHRGHGRSGGIRGHISSIEDFLQDIDQLLSQART
                     RYPNLPVILYGHSLGGILVLNYSLNRKPDIKGVIATSSGLHTALEEQPVKIFMAKLLG
                     SLMPKTTLPSGLDAKAISRDEAVVQTYNNDPLVHDKISLGFGKIMLGVTQWTLEHASE
                     FPLPLLLLHGKSDTIAYPSSSTEFAAALKDRATLVLWDGAYHELHNEPEKAEVFKTML
                     MWMDARLRG"
     gene            951..1691
                     /locus_tag="PLG52_01395"
     CDS             951..1691
                     /locus_tag="PLG52_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNB40121.1"
                     /translation="MTKEMSKRQLRKEQIRRKEKQSRFLAIGLITVGALFLAFLVIYP
                     NLKPLGEVVTPKEITRANVDFNTAGDPNAPITITEYSDFQCPYCRIFFENTEELLMKQ
                     YVETGTVHFVYKSVGAFIGPESKAAAEAAYCAGDQGKFWEMHDMLFTNQTGENVGAFD
                     DRHLEAFADELGLDRGQYDDCRSSGKYSDKAEQDAKDATAAGIQATPSFIITYDVNGE
                     TQTRVIQGAQGIDVFAQEIEAALADMGK"
     gene            complement(1740..2408)
                     /locus_tag="PLG52_01400"
     CDS             complement(1740..2408)
                     /locus_tag="PLG52_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB40122.1"
                     /translation="MRHHRTSILIADDHEVVRSGIRSYLEKISDFQVVGEASSGEETL
                     SLVSELIPDIVLLDLILPGMDGIETTRRIRQISPRTKVVVLTSYHEDVHIFPALKAGA
                     ISYILKDMKMEKLVEVLHRAVQGEVTLHPVVASRVLQNIRGENGEEQPLFTELTDRET
                     DVLKLIAAGLTNGQIADKLFISENTVKGHVSNILSKLHLADRTQAAVYAWQQGIVNRQ
                     STTK"
     gene            complement(2410..6165)
                     /locus_tag="PLG52_01405"
     CDS             complement(2410..6165)
                     /locus_tag="PLG52_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB40123.1"
                     /translation="MRKSWLVWILPFLFVLESCGTIQPATTPDARPNASLTDPLPARF
                     SMGPTTIPYGKNIRFEQISLEEGLSQSVVNVILQDQKGFLWIGTDDGLNRYDGYNFKI
                     FKPDTTNPFSLSDRSITDIVEDKDGNLWIATRMGGLNRYDPISGKFTQYMHSKNDATS
                     IISNQVTSLFVDKTGLWVGTNNGLDFLDFSSNQFIHYPLATGKLVPESKSISVVYKDS
                     ANRLWIGTSDAGLGLYDLKDQSFKAFKNNEYNNQSLSHNRILAIKQDLAGNIWVGTSN
                     GLNLYNEEEGDFTRFKRSKDDPNSIAGNIIYSLFTDQYGSLWVGTNVGLDRYSPQEGN
                     FIHHQYQPNVPNSLSNNEVLYIYEDDSGVLWVGTYGGGLNKYNRQQDRYAYFRNNPDD
                     PASLSSNIIFAIQPAEFGKVWVGTLDSGLNLFEPYTETFTRFVHDPNTPNSLINNSIF
                     SLYRDKAGLIWVGTGNGLDRYNRGSKSFTHYSPNESDFTRTDERFNVLAILEDTQKNF
                     WVGSSMGLYLFDRKAGTLTRHNSELGTTRVNALLEDKDGNLWVGTPEDGLRRLNTQTG
                     EISYFRYDSTEKTSLSNNSVLCIYQDPQGTIWIGTHGGGLSRFNPETETFTHFTEQEG
                     LSNNVVYGILEDESGKLWLSTNFGLARFNPINQTFRIFTASDGLQSNEFNQNSYAKDE
                     NGTMYFGGINGLNVFTPQEITDNQYTPKLSLTSITVDGTPFTNKDGRTTEYLDTITLT
                     WPQDSFEFEFAAFAFEQPLNNRYAYKLEGFDTDWISTDKQRNGRYTNLPGGTYTLRLR
                     GSNNDGVWNDQGASIQVVVVPPLWETWWFRGILVVAFMFSVVGGLRWRVKSVENKNLE
                     LERLVKIRTADLEKRTGEIEALYQADERILRSVTLNQIFQALVDVSISILKADRSMVL
                     TWNDEKRKITPRISLGFRPETLAVMNFDYDEGLIGISMKTGHPMIASEENLSSLREDT
                     RKAIEVEGIRSFAHFPIVVDEKVIAIFNVAYLRSNALTKDSIRLFTALVNRAAMAIAN
                     MELFEQTRDLAVMEERNRLARDLHDSAKQKAFAALAQLGTVNGMAKAVPAGPVVPIGA
                     EQHLSEAENLVYEVIQELNFLIQEIYPIALQEKGLPTVLREYIFEWENRNDIVANLMI
                     QNERRLQLETEQAIYRVIQEALANVSRHSQAKRVDISLVYNPDLLQVAMADDGCGFNV
                     NQKATGLGFRSMRERIISVRGTFQIQSAPGQGTRIIIQIPLKANSGG"
     gene            6548..9139
                     /locus_tag="PLG52_01410"
     CDS             6548..9139
                     /locus_tag="PLG52_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB40124.1"
                     /translation="MKIKKAFNALSLLVILGMLLAACAPAATDAPVEAPVATDEAMAT
                     DEPAPAATEAAPATTRHGGWLDEIDFSVVSSDSAVSQLQAGAIDFYSFNLSSNTLQDI
                     KDAGLNYTQSYGGNYGISLNPAVFDDTTVLNPFSNRKIREAVNWLIDRNYINQEIYGG
                     GSLPKLLPITTQLVEYTNLIDTARALETKYAYNLERAREQVAAEMEAMGATMSSDGKW
                     QFNGEPVTLIFLIRSDGDGTRKPMGDYVANQLEEVGFTVDRQYKTSSEAFPIWLGSVA
                     SEGQWHLYTAGYLPSGLTRDERANIQQYYLPTSIQASDPFISNVADPELQKLGDDLAQ
                     GNFTSKEERDAMLARALDLALQDSLFVWVVDQLVYAPYNSNVSVTYDLAAGYEAAFMG
                     NYNLRFVDQEGGTMKVGTNDLFTQPWNTMAGSNWVWDSNIMRATSMGVASSGSASSGL
                     MGDPYTGLAWPQRIESASLEYQTGLPITDNKLGWVDVQAVDEIPVPEDAWVDWDATTQ
                     TFITVGQKYPDGTTAKVKSTVVYPADLFETVKWHDGSPISVADFVMSFVIFLDRGKPE
                     SAVYDEAAALSVDAFLPSFKGWRITSTAPLTIEYYSDAYSADAELNVAPLWPGSPSGL
                     SGENSWQILAISNLAEAAGELAYSSDKADANSIEQMSWVGGPSLEILKKYLDQAQTES
                     YVPYEATLGQFLTPEEIALRYENFSKWYADHGHFWVGTGPYYLDQVFTTEKSLVLKSF
                     ADFPDLADRWSSFSDPKRARVILDGPGQVTAGQEATFDVTVNFGDEAYPTADIKQVKY
                     ILYGTTGDVLEVGNAEAVGEGQFQVALSADATSKLETGSAKLEVAVVPIPVAIPAFTS
                     FDFVVVK"
     gene            9209..>10029
                     /locus_tag="PLG52_01415"
     CDS             9209..>10029
                     /locus_tag="PLG52_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40125.1"
                     /translation="MSVVAPAASEQTAPKRKTALHALSRMLRYIAMRVLTLFTTVVIG
                     VYLTILIANMGGYVDQIIRGQIRDTITQSVANNPTLRNMDPETRRKLIEEQITLEEKR
                     RGLDTPIAIRNFRHLTQALQLDLGRAFNITSDSGSKQVRLILLERLPATLLLMGTSQL
                     FLFFSSIYIALNLSRNYGNIWDKLVIALSPTSAIPSWFYGIFLILIFAAGFKVLPFGG
                     MVSSPPPLNRFDYIISVLQHLVLPGGALVISSFFLSVYNYRTFFLIYSSEDYVDMA"
BASE COUNT         2552 a         2288 c         2362 g         2827 t
ORIGIN      
        1 acgattggag catcatagac atgaaacatc aagaatttaa ttggaaagca aaagacgggc
       61 ttgatatttt cgctcaggtt tgggagccag atgtggtgtt accccgtgcc gtggtgtgct
      121 tagtgcacgg cttgggtgaa catagttcac ggtatgctca tgttgccgaa gcatttagca
      181 aagaaggata cattctcttc tccttcgatc accgcggaca tggtcgctct ggcggtatac
      241 gtgggcatat ctcttctatt gaagacttcc tacaagatat agatcagtta ttatctcaag
      301 caaggactcg atacccgaac ctgcctgtta ttctgtatgg gcatagctta ggtggtatcc
      361 tagtcttgaa ctacagtttg aatcgcaagc cggatattaa gggtgtcatt gctaccagtt
      421 ctgggcttca taccgctttg gaagaacaac ctgtaaagat atttatggca aaattgcttg
      481 gatctctgat gcccaagaca acccttccta gtggtttgga tgcaaaagcc atctctcgtg
      541 atgaagcagt tgtgcaaacc tacaataatg acccgcttgt gcatgacaaa atctctctcg
      601 ggtttgggaa gattatgctt ggggtcactc aatggacttt ggagcatgct agcgagttcc
      661 cacttccgtt gttattgcta cacggtaaga gcgatactat tgcgtatcct tccagcagta
      721 ctgagtttgc cgctgctctg aaagaccgtg ccacccttgt tctttgggat ggtgcttatc
      781 atgaacttca taacgagccg gaaaaggcag aagttttcaa gaccatgctg atgtggatgg
      841 acgctcgcct gcgaggctga tcgaaaataa gaacctgtta acccatttct ggacaaatct
      901 attttggcat gagaaaatgc aatgccgata aaaaaataat aggagctagc atgacgaaag
      961 agatgagtaa acgccaacta cgcaaggagc agatccgtcg caaagagaaa cagtcccgct
     1021 ttttagcgat tggtttgatc accgtgggtg cgctatttct tgcattttta gtgatttacc
     1081 cgaatttgaa acctcttgga gaggttgtta ccccaaaaga aattacccgc gccaatgttg
     1141 attttaatac tgctggtgac ccgaacgcgc cgattacgat caccgagtat tctgacttcc
     1201 aatgtccata ctgccgtatt ttctttgaga atacagaaga attattgatg aaacagtatg
     1261 tggaaaccgg gacggtccac tttgtttata agtctgtagg tgcatttatt ggacccgaat
     1321 ccaaggcggc ggctgaagct gcttattgtg caggcgacca gggaaaattc tgggaaatgc
     1381 atgatatgct tttcaccaat caaacgggtg agaatgtcgg tgcttttgat gaccgccatt
     1441 tggaagcctt tgcagatgaa cttggcttgg accgcggtca atatgatgac tgtcgatctt
     1501 ctggcaagta ttctgacaaa gcggagcaag atgccaaaga tgccacagcg gcaggcattc
     1561 aggcaacgcc ttcatttatc attacctatg atgtgaatgg tgaaacccaa actcgggtga
     1621 tccagggtgc tcaagggatt gatgtatttg ctcaagagat tgaagccgct ttagcggata
     1681 tgggtaagta aatttcataa atacaaaaag acaccgcttt caaagcggtg tctttttgtt
     1741 tatttcgtgg tgctttgacg atttacgatt ccttgttgcc aggcataaac cgctgcctgt
     1801 gttcgatctg caagatgcag cttgctaaga atattgctga catgtccctt gaccgtattt
     1861 tcactgatga acaatttatc agcaatctgt ccattggtga gcccggctgc aattagtttc
     1921 agtacatcgg tttcgcgatc ggtcagctcg gtgaagagcg gctgttcttc accattttcg
     1981 ccgcgaatgt tttgaagtac acgtgaagcg accactgggt gaagtgttac ttcgccttgc
     2041 acagcacgat gcagaacttc aaccaatttc tccattttca tatctttcag aatataagag
     2101 attgccccag ctttcaatgc agggaagata tgcacatctt catggtaaga agttaacact
     2161 actacttttg tgcgcgggct gatttgccga atgcggcgtg tagtttcgat gccatccata
     2221 ccgggaagga tcaagtcaag aagaacaatg tcggggatta actcagagac caaactcaga
     2281 gtctcttctc cggatgaagc ttcacctaca acttgaaaat ctgaaatttt ttcaaggtaa
     2341 gaacggatgc cacttcgcac tacttcgtgg tcatctgcga tcaaaatact tgtgcggtga
     2401 tgtctcatgt taacctcctg aattggcttt gagagggatt tgaataataa tacgcgttcc
     2461 ctgcccgggc gcactttgga tttggaaggt gccgcgtaca ctgataatac gttctcgcat
     2521 ggaacggaaa cccaagccgg tcgctttttg gttgacattg aacccgcaac catcgtctgc
     2581 catggcaact tgaagaaggt ctgggttgta taccaatgaa atatctacac gttttgcttg
     2641 gctatggcgg gaaacatttg ccaacgcctc ttgaatcacg cggtaaattg cttgctctgt
     2701 ctcaagctgt agcctccgct cgttctgaat catgagattg gcgacaatat cgtttctatt
     2761 ttcccattca aaaatgtact cacgcaaaac agtgggcaat cctttttctt gcaaagcgat
     2821 tgggtaaatc tcctggatta aaaagttcaa ttcctgaatc acttcgtaga ccagattttc
     2881 ggcttcactc aaatgttgct ctgctcctat agggacgaca ggacctgctg gaacagcttt
     2941 tgccatgccg tttacggttc ctaattgtgc gagtgcagca aaggcttttt gtttggcaga
     3001 gtcatgcagg tcacgcgcga ggcggtttct ttcttccatc actgccaggt cacgagtctg
     3061 ttcaaatagc tccatgtttg caatcgccat ggctgctcgg ttcaccagcg cggtaaagag
     3121 acgaatggag tctttggtta aagcgttaga gcgtagataa gcaacattga aaatcgcgat
     3181 aaccttttca tcgactacga tggggaagtg ggcgaaagac cgaatgcctt caacttcaat
     3241 ggctttgcga gtgtcttcac gcagcgacga taaattttct tcagaagcga tcatgggatg
     3301 accagttttc atagaaatac cgatcagacc ttcatcgtaa tcaaagttca tcacagcgag
     3361 agtttcaggg cggaaaccca gactaatgcg tggagtgatc ttccgctttt catcattcca
     3421 ggtaagcacc atgctacgat ctgctttgag aatggaaatg gacacatcca ccagagcctg
     3481 aaatatctgg ttgagtgtga cgctgcgtaa aatccgctca tcagcttgat acaaagcttc
     3541 gatctcgcca gttcgttttt caaggtcagc tgtgcgaatt ttgaccagcc gttctagttc
     3601 cagattttta ttttccacac tttttactcg ccagcgtaaa ccacctacta cgctgaacat
     3661 gaaagcaaca actagaatgc cacggaacca ccaagtttcc cagagaggtg gtaccactac
     3721 cacttgaatc gatgcacctt ggtcgttcca aactccgtca ttgttggaac cgcgcaggcg
     3781 taaagtatag gttccgccag ggaggttggt gtacctgccg tttctttgtt tatcagtgct
     3841 gatccaatcg gtatcaaacc cttctaactt gtaggcataa cgattattga gaggttgttc
     3901 aaaagcaaac gctgcaaact caaattcgaa ggagtcttgg ggccaggtga gggtgatggt
     3961 atcaagatac tcggtggtgc gaccatcctt atttgtgaaa ggtgtgccat ctacagtaat
     4021 agaggttaat gagagttttg gcgtatattg gttatctgta atttcttgag gcgtaaagac
     4081 attaagccca ttgatgccac caaagtacat ggtgccgttt tcatcttttg cataagagtt
     4141 ctggttgaat tcgttgcttt gtaaaccatc gcttgccgta aaaatacgga aagtttggtt
     4201 gatcggatta aaccgggcaa gcccaaaatt tgtgctcaac caaagctttc cggattcatc
     4261 ttccaaaatt ccatatacaa cgttgttcga tagaccttcc tgttcagtaa aatgggtgaa
     4321 ggtctctgtc tctgggttaa aacggctcaa accgccgcca tgtgtcccaa tccaaatagt
     4381 gccttgtgga tcttggtaga tacataacac gctattgttg ctcaacgaag tcttttctgt
     4441 tgagtcatat cggaaataag atatttcacc ggtttgggta ttaagacggc gtaacccatc
     4501 ttctggtgta cctacccata gattgccatc tttatcttct aaaagcgcat tgactcgtgt
     4561 ggtgccaagc tcgctattgt gcctggtaag agttccagct ttcctatcga aaagatagag
     4621 ccccatgctg gaccccaccc aaaagttttt ctgcgtgtct tccagaatgg ctaaaacatt
     4681 aaaccgttca tcggttcttg tgaaatcact ctcgttggga gagtagtgcg taaagctttt
     4741 cgagccgcga ttgtagcggt ctagcccatt ccctgtgcca acccagatca agccggcttt
     4801 gtctctatag agagagaaga tgctgttgtt gataaggctg ttgggtgtgt tcgggtcgtg
     4861 gacaaaacgt gtaaaggtct ctgtgtacgg ttcaaataga tttaacccgc tatcgagagt
     4921 gcccacccaa accttcccaa actctgcggg ttgaattgca aaaatgatgt tgctgcttaa
     4981 gctggcaggg tcatcgggat tattgcggaa gtaagcatac cgatcttgtt ggcggttgta
     5041 tttgtttaat ccgccgccgt aggtgccaac ccataacact ccgctatcat cttcgtagat
     5101 atataacact tcgttattgc tcaaactgtt tggcacgttt ggctgatatt ggtggtgaat
     5161 aaagttacct tcttgcggag aataacggtc taaccctaca tttgtcccaa cccataaact
     5221 tccgtattgg tcagtaaata aggagtaaat gatattgcct gcaatactat ttgggtcatc
     5281 tttcgatctc ttaaaacgag tgaaatcacc ttcttcttcg ttgtagagat tcaatccatt
     5341 actagtgcca acccagatat tgccagcgag gtcttgtttg atcgcaagaa tgcgattgtg
     5401 actcaagctc tgattgttgt actcattatt tttaaacgcc ttgaaagact ggtccttcaa
     5461 atcataaagt cctaagccag cgtctgatgt gccaatccaa agccggttgg cagagtcttt
     5521 atatacaaca gaaatggatt tactttcagg gacgagcttg cccgtagcga gcggatagtg
     5581 aatgaattgg ttggatgaga aatcaagaaa atcgagccca ttgtttgtgc caacccaaag
     5641 cccggtttta tctacaaaaa gtgatgtgac ttgattgctg ataatactgg tcgcatcgtt
     5701 tttactatgc atgtattgag tgaacttacc cgagatgggg tcgtaacgat ttaatccccc
     5761 catacgagtg gcgatccaca aatttccatc tttgtcttca acaatatcag tgatcgaacg
     5821 gtcacttaaa ctgaatggat tggtagtatc aggtttgaat atcttgaagt tgtagccatc
     5881 ataacggttc aacccgtcat cggtgccaat ccataaaaaa cctttttggt cttgcaagat
     5941 tacatttaca acactttgcg aaagaccctc ttcaagacta atctgttcaa agcggatgtt
     6001 ctttccgtat gggatggtgg taggacccat agagaagcga gcaggcaacg ggtccgttag
     6061 agaggcgttt ggtcttgcat ccggtgtggt ggcaggctga atcgtcccac aagactccaa
     6121 aacaaaaagg aaggggagta tccataccag ccacgattta cgcatagtga aactattata
     6181 agcaatttat gagctgggat tgaaaggcgg ctccctctgg gggaggtctg tttcacccta
     6241 ccggagaatg gtttttcgct gtgagatggg ttgtgaaaca tttttcagtg gatggcttta
     6301 gaaccattca attgtgggag gaaaaccaat tgtctggatg catgacagtt gcccttggaa
     6361 ttcgtatgat aaggcgcatg tggaattgtt ctcctccaag aagaatcaaa ttccctcaga
     6421 aggcggagta tcagtgagca gactggtttt ccgccttctg cttagacgaa ttcattagga
     6481 ggtgctagct gacctatctt gtactctgtt caatttgttt tccaatttca ttaaggagaa
     6541 aaagagaatg aaaataaaaa aagcgtttaa tgcactcagc ttattggtca tccttggcat
     6601 gttgctggct gcttgtgcgc ctgccgctac cgatgcccca gtggaagcac ccgttgcaac
     6661 tgatgaagca atggcaacgg atgaacctgc acctgctgct acagaagcgg cacctgctac
     6721 gacccgtcac ggcggctggt tggacgaaat tgatttttca gtcgtttcat ctgattcggc
     6781 agtttctcaa ttgcaagccg gtgcaattga cttctattcg tttaacctgt cttcaaatac
     6841 cttacaggat attaaagatg ccggcttgaa ctacacccaa tcgtatggtg gtaactatgg
     6901 catcagttta aaccctgccg tgttcgatga cacaacggtc ttgaatccct tctcgaaccg
     6961 caagatccgt gaagcggtga actggttgat cgaccgtaac tatatcaatc aggaaatcta
     7021 tggcggcggt tccttaccaa aacttctgcc tatcacaacc cagttggttg aatacaccaa
     7081 tttgatcgat accgctcgcg ctcttgaaac caagtatgcc tacaaccttg agagagctcg
     7141 cgaacaggtt gccgctgaaa tggaagccat gggtgccaca atgagctctg atggcaaatg
     7201 gcagtttaat ggggaacctg tcaccttgat cttccttatc cgctccgatg gcgatggcac
     7261 ccgtaaaccc atgggcgatt atgttgccaa ccagttggaa gaagtgggct ttactgtaga
     7321 ccgtcaatat aagacatctt ctgaagcctt cccaatctgg cttggctccg tagcttctga
     7381 aggacagtgg catctttata ctgctggtta tttgccctct ggtcttaccc gcgatgagcg
     7441 cgcgaacatt cagcaatact atcttcccac cagtattcag gcgagcgacc cgttcatctc
     7501 caatgtagcg gaccccgaac ttcagaaact tggtgatgat cttgctcaag gtaacttcac
     7561 cagtaaagaa gaacgtgatg ccatgttggc gcgcgcctta gaccttgccc ttcaagactc
     7621 actctttgta tgggttgtag atcaactggt ctatgctcct tacaacagca atgtctcggt
     7681 aacctatgac ctggcagctg gttatgaagc ggcattcatg gggaattaca accttcgttt
     7741 tgtggaccaa gaaggcggca caatgaaggt cggtacgaat gacctcttca ctcagccttg
     7801 gaacacaatg gcgggcagca actgggtgtg ggattcgaac atcatgcgtg ctaccagcat
     7861 gggtgtggca agctccggca gcgcctcttc tggcttgatg ggtgacccat acaccgggct
     7921 tgcctggccg caacgtattg aaagcgcctc tcttgaatat caaactggtt tacccattac
     7981 tgataacaaa cttggctggg tagatgttca agctgtggat gaaattccgg ttccggaaga
     8041 tgcttgggtc gattgggatg caacgactca gaccttcatt acagtgggtc aaaaataccc
     8101 agatggcacc actgccaagg ttaagagcac ggtggtgtac cctgccgatt tgtttgaaac
     8161 cgtcaaatgg catgatggca gcccaatctc tgtcgcagat tttgtcatgt cgttcgtcat
     8221 tttccttgat cgtggtaagc ctgaaagtgc agtctatgat gaagcggcgg ctctttccgt
     8281 ggatgctttc ctgccttcct tcaaaggttg gcgtatcacc tctaccgccc cgctcaccat
     8341 tgaatactac tctgatgcgt actctgctga tgctgagttg aatgttgctc cattgtggcc
     8401 cggttccccg tcagggcttt ctggtgagaa tagctggcaa attttggcga tctccaatct
     8461 ggcagaagct gctggcgaat tggcttactc ttccgataag gcagatgcca atagcattga
     8521 gcaaatgagc tgggtaggcg gaccaagcct tgagatcctc aagaagtatt tggaccaggc
     8581 tcagaccgaa agctatgttc cgtatgaagc gaccttgggt cagttcctga ctccggaaga
     8641 aatcgctctt cgctatgaaa acttctccaa atggtatgcc gaccatggac atttctgggt
     8701 gggaactggt ccgtactatc ttgatcaggt ctttactaca gagaagtctc tcgtgctcaa
     8761 gagctttgca gacttccccg atcttgctga ccgctggtct tctttcagcg acccgaaacg
     8821 ggcacgagtc atcttagatg gaccgggtca ggtaacggcg gggcaagaag cgaccttcga
     8881 tgtgaccgtc aactttggcg atgaagcgta tccgactgcc gatatcaagc aggtgaaata
     8941 cattttgtat ggcaccacag gcgatgttct tgaagttgga aatgctgaag cagtgggcga
     9001 gggacagttc caagttgcct tgagtgccga cgcgacctcc aaactggaaa ccggttccgc
     9061 caaactcgaa gtggcagttg taccgatccc agtggctatc cctgctttca cttcctttga
     9121 tttcgtagtt gtaaagtaag ttgatgtaga aattccaata attcagggtg aggcatttcg
     9181 cctcaccctg aacccttgcg aggtatttat gtccgttgtt gctcccgctg cttctgaaca
     9241 gaccgctcca aagagaaaaa ccgcccttca tgctctaagc cgtatgttgc gttacatcgc
     9301 catgcgtgtg ttgaccttat ttactactgt ggtgatcggg gtttatctta cgatcttaat
     9361 cgcaaatatg ggcgggtatg tagaccagat cattcggggg cagatccgcg atacgatcac
     9421 acaatctgtt gccaataatc ccaccctgcg caacatggac ccagaaacac gccggaagtt
     9481 aatcgaagaa caaatcacac ttgaagaaaa aagacgcgga ctggatactc cgatagccat
     9541 acgtaacttt cgccacttaa cgcaagcact tcaacttgat cttgggcgtg ccttcaacat
     9601 taccagtgac agcggctcga aacaagttcg gctcattttg ttggaacgac tgcctgctac
     9661 attattacta atgggcacat cgcaattatt ccttttcttt tctagtatct atattgcact
     9721 gaatctttct agaaattatg gaaacatatg ggacaagctt gtaattgcac tatcgcccac
     9781 ctcagcaatt ccctcttggt tttacggtat tttcttaatt ctgatctttg cggcgggctt
     9841 taaagtcctt ccgtttggtg gcatggtctc ttcgccgccg ccgttaaatc gctttgatta
     9901 tattatcagt gttctacagc acctagtctt acctggtggt gctctggtca ttagttcatt
     9961 ttttcttagt gtttacaact atcgaacttt ctttttgatt tattccagcg aagattatgt
    10021 tgatatggc
//
LOCUS       DAOQNJ010000024         9990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3198_length_9990_cov_9.495924, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000024 DAOQNJ010000000
VERSION     DAOQNJ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3198_length_9990_cov_9.495924"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLG52_01420"
     CDS             <1..257
                     /locus_tag="PLG52_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB40126.1"
                     /translation="PPDDTATYNLLREHLGEVLNGLPPREVRILQLRYGLLDGQAYTL
                     EEVGRKMGVTRERVRQIEAQALSRLRHPSIRRKLRDYLGE"
     gene            complement(272..1216)
                     /locus_tag="PLG52_01425"
     CDS             complement(272..1216)
                     /locus_tag="PLG52_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40127.1"
                     /translation="MRTSDSNKIMWVAAIGAAIGLIAASFYLPGGDDLYRYYLPFAQG
                     CLHCGFMPYFAQWFLAPLRWLPEYPFSWGVWSLFSVSGILILAYYTKANPFLLLISFP
                     ALGQVWLGQVDIFIATGLVVFLFNKNPYLRGLGLIVALSKPQLTALPIFFCLLMEEDP
                     RLFLKLFLIPSIIILLSFIFFGVNWIPQWILETRSNLPIHVWRLASLDVWKFGIFLTP
                     IPILFREKRDRMLSSLLISALATPFYGVYSYLVFLLFEVKWWHVVLSFAWMIGFLFLH
                     DSAMRLAWILPLAMLVEMLYKKFKPKGIEDVSQKNRPS"
     gene            1226..2650
                     /locus_tag="PLG52_01430"
     CDS             1226..2650
                     /locus_tag="PLG52_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40128.1"
                     /translation="MNSENKSLEKFLRSISFLLTFFLLSRAPIDADLWWHLRAGQLMW
                     QKGEILLNDVFSYTKLGSNWVNAFWVSEVLLYWIHRIGGYFLLSFFVSLIGAITFLIV
                     FSSMKGNSLLRSFILILAVITAAPIWGPRPQILSFFLVAFLDLWLNQPSRHRWLLMPI
                     FALWANIHGGWIWGFLLLIAHIAGLFVNSFFVEDKKKLRTEGLHLIGWTVLSALAVGI
                     NPNGIYIWKLPFQQVNVSLQIQEWLSPDFHRLDFHPMLWMLFLLILLAPFAQKPLQMG
                     QILKTIGFAYLTFVAQRNVALFAIVAAPLLADWANNIIQLIPLSLNSPTGSSALKPAF
                     QKFINGFILIILTLTALGNLYVITQNSRIDSNYPVNGVAWLREHEPQGKLFNSYNWGG
                     YLLWTLPEYSVFIDGRADLYGNEILGQWQDVVNARENAEEILNTWQVNIVFIEPYWDI
                     VPVLKEKGWHVKYEDKSSIILIRP"
     gene            complement(2704..4020)
                     /locus_tag="PLG52_01435"
     CDS             complement(2704..4020)
                     /locus_tag="PLG52_01435"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNB40129.1"
                     /translation="MKYSLKTSFASYTALLVVVVFALTVVGRLVTITGAAFVCEGWPL
                     CLPTAPIGYLKLMHLGLVGLASIVMFLVWRKAWREQRHHAILLPLTTITGVMFIGQAF
                     VGAIEVTRQYPTHIVVLHTLTAIGLWISLAMLVFVSGTLEKDGVADYQFDWRQRVKDF
                     FILSKPVIVLLLLVTTYGGLVAGGKAWPSASLTFWTLFGGALAAAGSSALNQYIDREL
                     DKNMQRTSKRPLADGRLTAAEGLSYGLALCLVSYYVMAGFVNLLAAMLSLAGIFYYVF
                     FYSVWLKKATVQNIVIGGGAGAIPPMVGWAAATGNLSLAAWILFAIIFMWTPPHFWAL
                     AIVRKNDYARAGVPMLPVVEGEEKTRKQILIYTVELIAVTLLLPIFNLAGTIYLISAI
                     VLGAFLFYAAWKVFREGGNKVAWMMYRWSSMYLAFIFLALILDSVL"
     gene            complement(4020..4739)
                     /locus_tag="PLG52_01440"
     CDS             complement(4020..4739)
                     /locus_tag="PLG52_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNB40130.1"
                     /translation="MFSRKWVLTTLLALAGTAVCIRLGIWQLDRLEQRRAFNHQVESM
                     RAAELLDLNSEVPEDIASMEWRAVTITGNYDFENQVALRNQYNAGLYGFHIITPLLFD
                     GGAVLVDRGWIPAESDSPPAEWRMYDEDEQVTVTGQIRLGHGKPAFGGVVDALPVDGS
                     KLNVWNNLDVEKISTQMPYPILNVYIQPNANVSDTVPPIPFQPTIELTEGSHFGYALQ
                     WFTFATILFVGYPFFLQKQDD"
     gene            complement(4741..5226)
                     /locus_tag="PLG52_01445"
     CDS             complement(4741..5226)
                     /locus_tag="PLG52_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40131.1"
                     /translation="MKRILWSFLLGLVLAAITTELAYFLLKKENREPGVIELVIPAGT
                     AEKVAAGQAPLEIPKDMRFVVGDTLKVINQDTENHQLGPLWIPANSSATQKLETAENL
                     VVECSFQAGSVIGITVQEPVTWRTRISGIFFAGFPLGMMFAVYSVVVVPKKKEDNEHP
                     S"
     gene            complement(5235..5846)
                     /locus_tag="PLG52_01450"
     CDS             complement(5235..5846)
                     /locus_tag="PLG52_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB40132.1"
                     /translation="MDKKLLWTGGGILAIVVIAGLLSFIFANPPSFRGTLYGEPFPSA
                     AEIELTKADGSIFRLSDQRGKVVLLFFGYTSCPDVCPTTLAEMKLVMDKLESDAEQVQ
                     VVFVSVDPDRDTPEKIQTYVEHFNPTFLGLSGSLSELEPIWSSYSITREAVESDSALG
                     VIINHTARLFLVDPQGQMHLSFAYQTPVDDIVHDIRLILDQEK"
     gene            complement(5909..6700)
                     /locus_tag="PLG52_01455"
     CDS             complement(5909..6700)
                     /locus_tag="PLG52_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNB40133.1"
                     /translation="MAHSQDKSSALGQGVVIFVYLAVLTLIEYFIAVTFNATALLLVV
                     AVIKAALVVYYYMHIYELNAESGEHEDENSYEYKTGTNRIGLWLFLLSDAFVFAGLLV
                     MRSNLLGLTRPHLNQFLGLGVTAVLLISSFFMNRGETAMKHGDVKAFMNNTIITFVLG
                     LGFLLGVVLVEWRLAAEEGLVASFGNPSVGPLGGVFYMMTGMHAFHVFTGLIFLFVVW
                     NNARKGVYSAEKHWGVEAAAVYWHFVDLVWIFFYPALYLIGTAVK"
     gene            complement(6720..8612)
                     /locus_tag="PLG52_01460"
     CDS             complement(6720..8612)
                     /locus_tag="PLG52_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB40134.1"
                     /translation="MKGFFSSALARGLFWQLIGTLVGAGLVTGIRALMGLNALDTFFF
                     TEPAWVLGGFFGALFFLAGSGVVSDWFKWARGIDTPDHHEDHHTGAAKYINVSLDHKV
                     IGVQYTITALLLIAIGGLFALIFRTELAASQLQFLTTDMKLFSQNGPQLYNTLMSLHG
                     MIMIVSILLGTAGFINYVVPLLLGAQDMAFPRLNAFSFWIAVPGAVLLLMSLFLGGFD
                     TGWTGYPPLSARAPVGMQMFFLGVFVAGWSSILGSLNVIVTVLRMRAKGMTAWRMPIL
                     IWASLATALIALTATQLIGLSFQLVMFQRLFGMGFFDPAKGGNPVLFQHLFWFYSHPA
                     VYVFILPGLGVISELLPVFVRKPLFGYRWIAMSSLGIALVGFVVWAHHMYTSGMNEYL
                     RVPFMYSTLLVAVPTGVKFFSWVATIWKGKISTPTPMLFVLGGIIVFLLGGLSGPPNA
                     TVSTDLQLQDTYWIVGHFHDTIFGGFVFPFFAAIYFWFPKATGRKMNETLGKIHFWLM
                     TPSFFALTLGMMYAGLLGMRRRIVDYDPALGIDSIHLVMTIGAFLIALSVLIFFFNFF
                     NSIKNGEKAEGNVWNSRSPEWQVPSPMPAHNYDKPFEVIGEPYDYGLNDGGFVKFIEP
                     AKNSKH"
     gene            complement(8634..9644)
                     /gene="coxB"
                     /locus_tag="PLG52_01465"
     CDS             complement(8634..9644)
                     /gene="coxB"
                     /locus_tag="PLG52_01465"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNB40135.1"
                     /translation="MAHFVIVGILVIVMTILTYFGLDAAGLATQMHPVAASAQAAPID
                     QLWHWEIIVISFLLSLIVVPIVYSLVVFRQKKGELKDGEHIEGNTALEIGWTVVPLLI
                     VVVFAYLGAYSLGEVRRVDPNAMIVNVKAQQFSWTFEYPEYGFITTQLYLPIDRQVVL
                     KMESADVIHSFWVPEFRIKQDVVPGRITEYRVTPSLEGSYKVRCAELCGTSHAYMEAP
                     VVVVNSDAYDAWIVEQIKIAEESKTPEGQGKLLATKNGCVGCHSADGTAMTGPTWFNL
                     YGSEVKLADGSTVTADDAFITESILQPTAKVVNGFPPVMPPYTLTDEEISYIIAYIKT
                     LK"
BASE COUNT         3081 a         2368 c         2187 g         2354 t
ORIGIN      
        1 ccccacctga cgacaccgcc acctacaacc tgctccgcga gcatctcggt gaagtcttga
       61 acggattgcc cccgcgcgaa gtgcgcatcc tgcagctccg ctacggtttg ctcgatggtc
      121 aagcctacac cctcgaagaa gttggacgca aaatgggcgt cacccgcgag cgtgtgcgtc
      181 aaatcgaagc acaagcatta agtcgcttac gtcacccgtc catccgcaga aaactgcgcg
      241 attatttggg cgagtaaaaa aacagaccat cttaggatgg tctgtttttt tgtgaaacat
      301 cctctatacc tttaggtttg aattttttat aaagcatttc aactagcatt gccaaaggca
      361 aaatccatgc caggcgcatt gcggagtcat gcaaaaataa aaacccaatc atccaagcaa
      421 aagaaagcac tacgtgccac catttcactt caaaaagaag aaaaaccaaa taagaataaa
      481 cgccataaaa cggagtagct aacgcagata tcaacaagct agataacatg cggtctcttt
      541 tttcgcggaa cagaataggt attggcgtca aaaatattcc gaacttccac acatctaaac
      601 ttgccaagcg ccagacatga atcggtaaat ttgagcgcgt ttctaatatc cactgtggga
      661 tccaattcac accaaaaaag ataaaactaa gcaaaatgat gatggacgga atcaggaaaa
      721 gtttaagaaa gaggcgcgga tcttcttcca tcaaaaggca aaagaaaatt ggcaaagccg
      781 tcaattgagg tttagataaa gcgacaatta gacccaaacc acgcaaataa ggatttttat
      841 taaaaagaaa aacgactagc cctgttgcaa tgaaaatatc tacttgccca agccaaactt
      901 gaccaagcgc aggaaaggaa atcaataaca aaaacgggtt agcttttgta taataagcaa
      961 gtataaggat gccagatacg ctaaacaaag accaaacgcc ccaggaaaat ggatattctg
     1021 gtaaccaccg tagtggagca agaaaccatt gcgcaaaata cggcataaag ccacaatgaa
     1081 gacacccttg cgcaaaaggc agatagtatc gatataggtc atcgccgccg ggtaaataaa
     1141 acgaagctgc gattagccca attgctgcac caattgcggc tacccacatt attttattag
     1201 aatcggatgt tctcaaaacg cctccatgaa ctcagaaaat aaatctctcg aaaaattcct
     1261 taggtctatt tcttttttat tgacattttt tttattaagc cgggcaccca ttgatgcaga
     1321 tttgtggtgg catttaaggg cagggcaatt gatgtggcaa aagggagaaa ttctattaaa
     1381 tgacgtcttt agctacacca agctaggttc aaattgggta aatgctttct gggtttctga
     1441 ggttttatta tattggatac accggattgg cgggtatttc ttactaagct tttttgtttc
     1501 gcttatcgga gctataacat ttctcatcgt attctcaagc atgaaaggga actcgctctt
     1561 aaggagtttt attctaatat tggctgtgat tacggcagca cccatttggg gacctcgccc
     1621 acaaatactt tctttctttc tagtggcatt tttagactta tggctaaatc aaccatcacg
     1681 gcatcgatgg ctactgatgc caatatttgc gctttgggca aacattcatg gcggttggat
     1741 ttggggcttc ctgctcctta tagctcatat tgctgggttg tttgtcaatt ccttttttgt
     1801 tgaggataag aagaagcttc gtacagaagg gctgcactta attgggtgga cagtcctttc
     1861 tgccttagcg gtaggtataa atccaaatgg catatatata tggaaattgc ccttccaaca
     1921 agtaaatgtc tcattacaaa tccaagagtg gttatcgccc gattttcatc gacttgattt
     1981 ccaccccatg ttgtggatgc tctttttatt aattcttttg gcaccatttg cgcaaaagcc
     2041 gctacagatg gggcaaatac tcaaaaccat tgggtttgcg tatctcacat ttgtcgcgca
     2101 gagaaacgtt gccttatttg caattgttgc agcaccacta ttagcagatt gggcaaataa
     2161 tattatccag ttaatcccgt taagtcttaa ttcgccaaca gggtcttcag cattaaaacc
     2221 ggcatttcaa aaatttatta atggatttat attgatcatt cttacattaa ctgcgctagg
     2281 aaatctttac gtcattactc aaaatagtcg aatagatagc aattaccctg ttaatggagt
     2341 agcttggtta agagagcatg aaccacaagg caaactgttc aactcttata attggggtgg
     2401 ctatctttta tggacattac ctgaatattc tgtatttatt gatgggcgtg cagatttata
     2461 cggaaatgaa atactaggtc aatggcaaga tgttgttaac gccagggaaa atgccgaaga
     2521 aatattaaat acttggcagg ttaatatcgt atttatcgaa ccctactggg atatagtccc
     2581 tgttttgaaa gaaaagggat ggcacgtaaa atacgaagat aaatcttcaa tcatcctcat
     2641 ccgcccataa aagaaaaagg ctagccaatt ttattagcta gcctttttag tatcaaatac
     2701 aggttacaac accgaatcca agatcaatgc cagaaagata aatgccagat acatgcttga
     2761 ccagcgatac atcatccaag caactttatt tccaccttct cggaacactt tccatgcagc
     2821 atagaagagg aaagcgccca agacaatagc agaaatcagg taaatcgttc cagctaaatt
     2881 aaagatagga agcaaaaggg ttacagcgat caactcaact gtatagatta ggatctgctt
     2941 acgcgtcttc tcttcgcctt cgaccacagg gagcatcgga acacccgcac gggcataatc
     3001 atttttgcgg acaatggcaa gtgcccagaa atgtggaggc gtccacatga agataatggc
     3061 gaagaggatc catgcagcga ggctcaggtt gccagtggca gctgcccagc caaccatcgg
     3121 cggaattgcc cctgccccac caccgatcac gatattttga acagtggctt tcttcagcca
     3181 aacgctgtag aagaatacat agtagaaaat tcctgccagc gaaagcatgg ctgctagcaa
     3241 attcacaaaa ccagccatta cataataact aaccaaacaa agcgccaatc catatgaaag
     3301 accctctgcc gcagtcaaac gcccgtctgc cagcggacgc ttcgatgtac gctgcatatt
     3361 cttatctagt tcgcggtcaa tatattggtt caaagcactg gagccagccg cagccaaagc
     3421 gccaccgaat aacgtccaga atgtgagcga agcagagggc caggctttac caccagccac
     3481 taagcctcca taggttgtca ccaaaagcaa caaaacaatc acaggcttgg ataagatgaa
     3541 aaagtctttg acccgttgac gccaatcaaa ttgataatct gccacgccat ctttttcaag
     3601 cgtgccagac acaaaaacaa gcattgccaa cgaaatccaa agcccgattg ctgttagtgt
     3661 atgaagaacc acgatatggg ttgggtactg gcgcgtcacc tcaattgccc ctacaaatgc
     3721 ctgcccaata aacataacgc ctgtgatcgt cgtcaatggc aacaagatgg catgatgccg
     3781 ctgttcacgc caagcctttc gccacacgag gaacatcaca atggatgcca acccaaccaa
     3841 gcccaaatgc atcaacttta aatagccaat cggagcagta ggcaaacata aaggccagcc
     3901 ttcacacaca aaagcagctc cggtaatcgt caccaaacgc cccaccacag tgagcgcaaa
     3961 aactacgaca accaacaagg ctgtgtagct agcaaaagaa gttttcaaag aatatttcat
     4021 caatcgtcct gtttttgcag aaaaaatggg taacctacga acaaaatcgt cgcaaaggta
     4081 aaccactgca aagcataccc gaaatgtgag ccttccgtca attcaattgt gggctgaaat
     4141 gggattggcg gaaccgtgtc actgacattg gcattgggtt gaatataaac attaagaatt
     4201 ggataaggca tctgtgtgga tattttctcc acgtcgagat tattccacac attgagtttg
     4261 gagccatcaa caggcaacgc atcgacaact ccgccgaaag ctggtttgcc atgcccaagc
     4321 cgtatctgcc ctgttaccgt gacttgttca tcttcgtcgt acatacgcca ttcagcaggt
     4381 ggcgagtcgc tctcagcggg aatccagccg cggtctacca gaacagctcc cccgtcgaaa
     4441 agaagcgggg tgatgatgtg gaatccatac aagcctgcat tatattggtt tcgcaaagcg
     4501 acctgatttt caaagtcata gttgccggtg atggtgactg cccgccattc cattgaagca
     4561 atgtcttcag ggacttcgct attgaggtcc aataattctg ccgcgcgcat actttccact
     4621 tgatgattaa atgcgcggcg ttgttcaagt cgatctagtt gccaaattcc caaacgaata
     4681 caaaccgccg ttccagctaa agccagcaac gtagtaagca cccattttct tgaaaacatt
     4741 ttacgaagga tgttcattgt cttctttctt cttgggaaca accactacac tgtaaacagc
     4801 aaacatcatt ccaagcggaa agccagcaaa gaaaatgcct gaaatccttg tgcgccatgt
     4861 aacaggttct tgaacggtaa tgccgatcac actcccggct tggaaagaac attccaccac
     4921 taaattttct gctgtttcca atttttgtgt agcagaagaa ttagcaggaa tccataacgg
     4981 acccagttga tgattttcag tgtcttggtt tatcactttc aatgtatcgc ctacgacaaa
     5041 tcgcatatct ttcggtattt caagcggagc ctgaccagca gcgacttttt cagcagttcc
     5101 cgcaggaata accaattcaa taacaccggg ctcgcggttt tcttttttca agagaaagta
     5161 tgccaactct gttgtaatag ctgccaatac aagccctaaa agaaatgacc ataaaattcg
     5221 cttcattgtg aaattcattt ttcttgatcc aaaatcaaac ggatatcgtg gacaatatca
     5281 tctacagggg tttggtatgc aaaggaaaga tgcatctgcc cttgtgggtc gacaaggaat
     5341 aaacgtgcgg tatggttaat aattacaccc agtgccgagt cactttctac ggcttcgcgt
     5401 gtaatggaat aactactcca gataggctca agttcagata aagatcccga tagacccaag
     5461 aaggtgggat tgaaatgttc tacataggtc tgaatctttt ctggagtatc gcggtctggg
     5521 tccacggaca caaaaacaac ctgtacttgt tccgcgtcac tttcgagttt atccatcacc
     5581 aatttcattt cggcaagagt ggttgggcag acatctgggc aagaagtgta gccgaaaaac
     5641 agcagcacaa cttttccgcg ctggtcgctc aggcgaaata tgcttccatc tgcttttgtc
     5701 aactcaatct cagctgcaga cgggaaaggc tcaccatata gtgtgccgcg aaaggagggc
     5761 gggttcgcaa agataaaaga aagcaaccct gctattacaa caatagcaag gatgcctccg
     5821 cccgtccaca gtaatttttt atccatattt tatctccgat taatgtcgaa aaaaaaccgc
     5881 cgcctcatcg gcggcggagg tattcatttt attttacagc cgtgccgatc agatacaagg
     5941 cagggtagaa gaaaatccaa actaaatcta cgaagtgcca ataaactgca gcggcttcta
     6001 caccccaatg tttttcagca gagtacacac ctttgcgggc gttattccac acaacaaaga
     6061 ggaagatcaa accagtgaaa acatggaagg catgcatacc ggtcatcatg tagaaaacgc
     6121 cgcccaacgg accgacagac gggttgccaa atgaagccac caaaccttct tcagctgcca
     6181 agcgccattc cacgagcacc acgccaagca aaaagcccaa gcccaaaaca aaggtgatga
     6241 tggtgttgtt cataaaggct ttcacatcgc catgtttcat agcagtctcg ccacggttca
     6301 tgaagaagga agatatgagc aacacagccg ttacacccaa acccaagaac tggttgaggt
     6361 gcgggcgggt aagcccaagc aagttagaac gcatcaccaa cagaccagca aacacaaatg
     6421 cgtctgacaa gaggaacagc caaagaccta tacgatttgt gccggtctta tactcataag
     6481 agttttcatc ttcatgctcg ccgctttctg cattcaactc atagatgtgc atgtagtaat
     6541 acacaaccaa agcggctttg atcactgcca caaccagcaa aagggctgtt gcgttgaatg
     6601 ttacagcaat gaagtattca ataagggtca gaactgccaa atatacaaag atcaccacgc
     6661 cctgaccaag agcggacgat ttatcttgtg aatgtgccat ttaattactc tcctcgcctt
     6721 tagtgcttgc tattcttggc aggttcaatg aacttaacaa agccaccatc attcaaaccg
     6781 tagtcatacg gttcgccgat cacttcgaat ggcttatcgt agttatgggc aggcatgggg
     6841 gaaggaacct gccattcagg ggagcgcgaa ttccaaacgt tgccttccgc cttctctcca
     6901 ttcttgatgc tattgaagaa gttgaagaag aaaatcaaaa cggaaagggc gatcaggaac
     6961 gcacctatgg tcataactaa atgtattgag tcaataccca aagcagggtc atagtccacg
     7021 atgcggcggc gcataccgag caagccagca tacatcatgc ccaaggttaa ggcaaagaac
     7081 gaaggggtca tcagccagaa atgaatcttg ccaagggttt cgttcatctt gcgtccggtt
     7141 gctttgggga accagaagta gatggcagca aagaatggga agacaaaccc accgaaaatg
     7201 gtgtcgtgga agtgccctac gatccaatag gtgtcttgta actgaaggtc ggtagagact
     7261 gtcgcattgg ggggacccga gagaccacct aaaaggaaga cgataatacc gcccaacaca
     7321 aagagcattg gggtaggagt tgaaatcttg cctttccaaa ttgttgcaac ccaggagaag
     7381 aacttaacgc ccgtcggtac agccaccaac aaggtgctat acatgaaagg tacgcgtaaa
     7441 tattcattca taccagaggt atacatgtgg tgcgcccaca caacgaagcc caccaaagca
     7501 atgcccaaag aggacatcgc aatccagcga taaccgaaga ggggcttgcg gacaaacaca
     7561 ggcagtaatt cagagatcac acccaagcca ggcaaaatga atacgtacac cgcagggtga
     7621 gagtagaacc agaacaggtg ttggaagaga accgggttgc cgcctttggc aggatcaaag
     7681 aagcccattc caaagaggcg ttggaacatc accaactgga aagaaagacc gatcaactgt
     7741 gtagcggtca gcgcgatcag tgcagtggca agagatgccc aaatgaggat cggcatgcgc
     7801 caagcagtca tgcctttggc acgcatacga agcacagtaa caataacgtt caaagaacca
     7861 agaattgatg accaaccggc aacgaacacg ccaaggaaga acatctgcat accgacagga
     7921 gcgcgggcag agagaggcgg gtagcccgtc caaccagtat caaaaccgcc gaggaaaagg
     7981 ctcatgagca gaagcacagc cccagggaca gcaatccaga acgagaaggc attgaggcgc
     8041 gggaatgcca tatcctgcgc accgagcaac aggggaacga catagttgat gaagccagct
     8101 gtgcccaaca agatcgagac gatcatgatc atgccatgca aggacatcaa tgtgttgtac
     8161 aactgaggtc cattctggct gaacaatttc atatcagtag tgaggaattg gagttgagat
     8221 gcagccaatt ctgtacgaaa gatcaaagca aataaaccgc caatggcgat caacaacaac
     8281 gctgtgatgg tgtactgaac accaatgacc ttgtggtcaa gtgaaacgtt gatgtacttt
     8341 gccgcgccgg tgtggtggtc ctcatggtga tctggcgtat caatgccacg cgcccacttg
     8401 aaccaatcgc tcacaacccc agaaccagcg aggaagaaaa gcgcaccgaa aaagccgccc
     8461 aacacccagg ccggttcggt aaagaagaac gtatcaagcg cattcaaccc catcaatgcg
     8521 cgaatgccag tgacaagacc agcaccaacg agcgtgccta tcaattgcca aaacaaaccg
     8581 cgagcaaggg cactagaaaa gaatcccttc atagtattta ttccttatca aatttatttc
     8641 aaggtcttga tgtatgcgat gatataagag atctcttcat cggtcaaagt gtaaggcggc
     8701 ataacgggcg ggaagccatt aacaacctta gctgttggct gaagaataga ctctgtgatg
     8761 aaggcatcgt cggcagtcac agtggagccg tcagcaagct taacctctga gccatacagg
     8821 ttgaaccaag tcggtcctgt catagccgta ccgtctgcag agtgacaacc aacacagcca
     8881 ttcttggttg caaggagttt accctgccct tcgggggtct tggactcttc ggcaatcttg
     8941 atttgttcaa caatccaggc atcatacgca tctgaattga caacgacaac aggagcttcc
     9001 atataagcat gggatgtacc gcaaagctcg gcacagcgga ctttgtagct gccctcaaga
     9061 ctcggagtaa cacgatactc ggtgatgcgt ccaggtacaa cgtcttgttt gatgcggaac
     9121 tcaggaaccc agaatgagtg gatcacatcc gcagactcca tcttaagcac aacctggcgg
     9181 tcaatgggca gatagagttg agttgtaata aacccatatt cggggtattc aaaagtccat
     9241 gaaaattgtt gggctttgac gtttacaatc atggcgttcg gatctacgcg gcggacttca
     9301 ccaagtgaat aagcgcctaa ataagcaaaa acaaccacga tgagtagcgg gacaaccgtc
     9361 caaccgattt caagggcagt attcccctct atatgttccc catccttaag ttcgcctttc
     9421 ttttggcgaa acacaaccaa actatataca atcggtacaa cgatcagcga aagtaggaag
     9481 gaaattacga tgatttccca atgccataac tgatcaatgg gagcagcttg tgcgctggct
     9541 gccacaggat gcatttgggt tgcaagccca gcagcatcaa gaccgaaata cgtgagaatg
     9601 gtcatcacaa tgaccaagat tcccacaatt acaaaatggg ccatatactc tctctcctga
     9661 aaagattgaa cggggcatga gctccgatga ttaactaaaa ttggacaaat acagttagaa
     9721 ttgtctcaat tttcacaata tcagattata acaaaatccc tttttattgt cacgctatct
     9781 tggtcaaatg cacagctatt cttaacttgc acaaacctcg attttcacaa atcatgccga
     9841 cggtgtttgg ataccgtcgg caatctaaaa gctatatcaa gctataacac gatacgttgt
     9901 cgtagagatg cgttatttca agcctaactc tgctaaaagt tcctggctgt gttcagctgg
     9961 gcgcacatct tcatatactt tcttgatgtt
//
LOCUS       DAOQNJ010000025         9888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3258_length_9888_cov_8.773009, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000025 DAOQNJ010000000
VERSION     DAOQNJ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3258_length_9888_cov_8.773009"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..1570
                     /gene="rho"
                     /locus_tag="PLG52_01470"
     CDS             293..1570
                     /gene="rho"
                     /locus_tag="PLG52_01470"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNB40136.1"
                     /translation="MKILELENEPLSKLRMMARDLNIQNANRLKKETLAMLIREAEAQ
                     KEGIELRGGILEIMSEGIGFLRATNYRISDQDVYVSQAQLRRYDLRAGDLVIGQVRPP
                     RESERHFGLLKVESINGLDPEDAIRRTVFENLTPIFPDKRFDLETDPKTLAPRLINLI
                     APIGRGQRGLIVSPPKTGKTTILKQIANSISTKYPDVHLIIALIGERPEEVTDMDRSV
                     EAEVVASTFDEPVTSHVRTAELALDRAKRLVEIGRHVVILMDSITRLARAYNLTVNPS
                     GRTLSGGMDPSALYPPKRFFGAARNVEEGGSLTIIATCLVDTGSRLDDVVYEEFKGTG
                     NMELHLSRKLQERRTFPAVDLERSSTRREDLLLGPDLLQRVWLLRRMFVQMTSSQPAG
                     AGMDPSVATEAILTRLEKSKNNIEFLENLTKDA"
     gene            1617..2792
                     /locus_tag="PLG52_01475"
     CDS             1617..2792
                     /locus_tag="PLG52_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB40137.1"
                     /translation="MKLFERIKSIFPARKKAKEEEQPQAVSAVNEPSTSPRKEVKKAD
                     RFTIVSWSVTSVLVVALLSGTLYYKSTLPATVVTVPAATEEPAADGSVPVVVNSGTGG
                     GGSEGLFPTILRKLQIKTNKPERPRYDPELYRVVRGDSMYRIAEDFKIKSETILYVND
                     QLEDNPHSLRPGMELTIPPADGLYYTWKEGDTFESVAEEYNANADDIINFTGNQIDLT
                     DPKVEVGTQIFIPGGSRELVNWAAQLPTNARGVNTGTGGGNAANVCGGGPVASGFGWP
                     ADDHTLSGNAYGPGHLGIDISAPEGANVYAAGTGVVTMAAGGWNYGYGNVVQIDHGNG
                     YVTVYAHLSSILVSQCQTVGQGSVIGLAGNTGNSFGAHLHFEIRVGGSNINPYDIVQ"
     gene            2839..3618
                     /locus_tag="PLG52_01480"
     CDS             2839..3618
                     /locus_tag="PLG52_01480"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNB40138.1"
                     /translation="MNESSLKKRLAKLNLGGLRYFESLGSTNDEALAWAARGADDFSL
                     VVADEQTQGRGRLDRKWFTPKGSAIAMSLILRPTAVMRSHLSRTVGLAALSLADACLK
                     LGLSPKIKWPNDILLDSKKTAGILVEMVWAGEDADSLVIGMGINVHKSAVPPRETLQF
                     PATSLEDMLGNAPKREDIIFQILSNVQRWREQIGTERFIQAWEEKLAFRGEAIQIHAG
                     GAESHQGVLDGLESDGSLRICDEHNKSVIVRFGDVSLRPTA"
     gene            3649..3873
                     /locus_tag="PLG52_01485"
     CDS             3649..3873
                     /locus_tag="PLG52_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40139.1"
                     /translation="MFDDLRNDAAKQYEDEVQAEYQPASVTSSGAPRQRSTKFLGMTS
                     IQRFILVFLLMTATCVIGFLCLFAFGKIAF"
     gene            4186..4689
                     /locus_tag="PLG52_01490"
     CDS             4186..4689
                     /locus_tag="PLG52_01490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB40140.1"
                     /translation="MNIYDKSIRDELTKAYNRRYFFEASKAIENNEALIPQNTSLLLI
                     DIDDFKLLNDTYGHDIGDLALKTLTEESSRLLRTTDVFARYGGDEFICLLPNTKQSQA
                     YEIATRLLESANKHPFYKKDRNIFLRLSIGIATTTSETKLNDLISLADKALYKSKKNG
                     KNTVEAL"
     gene            complement(4728..7244)
                     /gene="gyrA"
                     /locus_tag="PLG52_01495"
     CDS             complement(4728..7244)
                     /gene="gyrA"
                     /locus_tag="PLG52_01495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNB40141.1"
                     /translation="MAEENMIAGNVESVDIDAQMRSAYLDYAMSVIVARALPDARDGM
                     KPVHRRILYAMHELGLRSNSSYKKSARIVGEVLGKYHPHGDSAVYESMARMAQDFSMR
                     YMLVDGQGNFGSVDGDAPAAMRYTEARLQKMAEEMLQDLEKETVDYGPNFDDSLQEPL
                     VLPARLPNLLLNGASGIAVGMATNIPPQNLRELTSAINYIIDNYDKADEITVDDLMKF
                     VQGPDFPTGGMIVGTEGIVSAYGTGRGRIVMRGIAHIEETKAGRYQINITEIPYQVNK
                     SSLIERIAELVREDKIDQISDLRDESDKRGMSIVIELKRGAQPKKVLNQLYKYTALQS
                     TFGVQMLALVDGEPRTLPLKRLLQIFIEHRQIVITRRSQFDLAKAKARLHILDGYLIA
                     LNNIDAIIKVIREAEDADKAKSALITRFKLSEIQAQAILDMQLRRLAALERWKIEEEH
                     KQVSETIKYLEDLLANPHKILALIKDDMTDVAEKYGDDRRTKISAEAREDIHEEDLVP
                     DESVLISLTERGYIKRVAATAFRQQSRGGRGVKGHTTKDEDEVVMLIPARSLDTMLFF
                     TDKGKVYSEKVYQIPDLDRAQKGIPLVNILALGPNEKVTAAMSVRDFLPTQFCIMMTG
                     RGRIKRVTMEEFASVRPSGLIAMNLDEGDTLGWVRLTSGKDEIIIVTENGQALRFSED
                     KVRAMGRQAAGVQGIRLGKEDAVTSMDVVDKEGALLVVTTNGFGKQTPLKEYTPKGRA
                     TGGISTIDQKALQEIGKIAAARVVNKDDDLTIMTANGVAIRLKVKDVKQSGRATRGVH
                     LIKPQEGDSVASVARISADDLKKAGAQIEEQEAEPQPKLV"
     gene            complement(7515..7598)
                     /locus_tag="PLG52_01500"
     tRNA            complement(7515..7598)
                     /locus_tag="PLG52_01500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7562..7564),aa:Leu,seq:cag)
     gene            8357..8644
                     /locus_tag="PLG52_01505"
     CDS             8357..8644
                     /locus_tag="PLG52_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40142.1"
                     /translation="MQITPLHTSIEKLIELKIGVVVMESPNGFPLHETNIYLVAPSGD
                     VVWKAEKPSANVLFTKMKLNEDGSISTFTNSGQFCDINGENGKVISSSNFS"
     gene            8663..9187
                     /locus_tag="PLG52_01510"
     CDS             8663..9187
                     /locus_tag="PLG52_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40143.1"
                     /translation="MPLHFTKLANPIPEEVYSAFTKWENDPALVHLTRPNQTQADIDR
                     KSEVDSFELDARISYQMTYLIYLDSQLVGEMSYQVDPQHLYKKETGTAWISITIGEES
                     ARGKGIGKLAMQYLEEEIHNAGYKRIELGVFEFNEPAQKLYQKMGYTEIGRIPNFTFW
                     QNKMWTDIRMEKYI"
     gene            9187..9471
                     /locus_tag="PLG52_01515"
     CDS             9187..9471
                     /locus_tag="PLG52_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40144.1"
                     /translation="MIKGFLNLPWFVWAGLALVVALIYTFWVPQKIAPDISGIRLLIV
                     RWAHPLTWYLLALNFILRGISPEWGGIANMVAAAGGLMYMLFMLMTFVIK"
     gene            9473..9751
                     /locus_tag="PLG52_01520"
     CDS             9473..9751
                     /locus_tag="PLG52_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40145.1"
                     /translation="MEKPPFSFVLIFLLFSFLFISNSYKLWFKTDEYYKDLYASLTNE
                     RIPFPFKSFFLARLEKKQSWLFWQKAFSLLGIVAVVGMDVLVVMAYLG"
BASE COUNT         2651 a         2416 c         2280 g         2541 t
ORIGIN      
        1 gtattaaaac cttttaggaa ttaaccagcc caagcgggga ctggtctttt cacattctca
       61 tcccaacaga tcaactcaca tgccaaataa acctacaaat acgtcaaatc gttcaaccaa
      121 ctctacaaga agaccctacc aacgttcgcc caaacccaat aatagaaaca attcttttga
      181 ttcaaatacc ccgcctgccc cggtggttga atctacaaac acgccagcca ttccgcctag
      241 cgtacccaat acccctgcct ctgaaaattc atatatgtcg agagagaaga aaatgaaaat
      301 acttgaactg gaaaatgagc ctctttccaa acttcgtatg atggcaaggg atctaaatat
      361 ccagaatgcc aatcgcctaa agaaagaaac tctcgccatg ctcatccgcg aggcagaggc
      421 acagaaggaa ggtattgaac ttcgcggcgg tattctcgaa attatgagcg aaggcatcgg
      481 ttttctgcga gccacaaact atagaataag cgaccaagat gtttatgttt cgcaagctca
      541 attaagacgt tatgatcttc gtgcggggga tttggtgatc gggcaggttc gcccccccag
      601 agaaagtgaa agacacttcg gtttattaaa agtagaatcc atcaatggct tagacccaga
      661 agatgccatt cgtagaacgg tttttgaaaa tctcacccca atctttccag ataagcgttt
      721 tgatttagaa acagacccca aaacattagc tccacgttta attaacttaa ttgcccccat
      781 tggtagaggg cagcgcggtt tgatcgtttc gccgcccaaa acggggaaaa ctacaatttt
      841 gaagcaaatt gcaaattcaa tttctacaaa atatccagac gttcacttaa ttattgccct
      901 catcggcgaa cgccctgaag aagtcaccga catggaccgc tcagtggaag ccgaagtggt
      961 cgcctccacc ttcgatgagc cggtcacttc gcacgttcgc accgccgaac ttgcattaga
     1021 ccgcgctaag agattggttg agatcggtcg acatgtagta atcttaatgg actccattac
     1081 ccgcctcgcc cgcgcctaca acttgaccgt caacccctct gggcgcaccc tctccggcgg
     1141 tatggacccc tccgcgttgt acccgcccaa gcgtttcttc ggcgcggctc gtaacgtgga
     1201 agaaggcggc tcgctaacca tcattgcaac ctgtctcgtt gacaccggtt ctcgtctcga
     1261 tgatgtggtg tacgaagagt tcaaaggcac cggcaacatg gagttgcacc tctcgcgcaa
     1321 attgcaggaa cgccgtacct tccctgccgt agacctcgaa cgttcgtcca cccgccgcga
     1381 agacctgctg ctcggacccg acctgctcca gcgtgtttgg ctcctacgcc gcatgttcgt
     1441 acagatgact tcatcccagc ccgctggcgc cggtatggat ccttctgtgg cgaccgaagc
     1501 cattttgacg cgcttggaaa aatcaaagaa caacatcgaa tttttggaaa atcttacaaa
     1561 agacgcataa cgaaaacaaa agttgaattg gtacaaaatc gtaacaccca ttaaaaatga
     1621 aactttttga aagaatcaaa tccatcttcc cggcacgcaa gaaagccaaa gaagaagagc
     1681 agcctcaagc tgtttcagca gtgaatgaac cctcaaccag tccgcgtaag gaagtaaaga
     1741 aagcggatcg ctttaccatc gtgagttggt ctgtgacttc ggtcttggtt gtggcattgc
     1801 tcagtggaac gctctattac aaaagcaccc ttcccgctac tgtggtgacc gtccctgcgg
     1861 cgacggaaga accggcggca gatggcagtg taccggtagt ggtcaactca ggcacaggcg
     1921 gaggaggaag cgaaggtctc ttccctacca ttctccgcaa attgcagatc aagaccaaca
     1981 agccggaacg tccgcgctat gatccagaac tttatcgcgt tgtgcgcggc gactcgatgt
     2041 atcgcattgc cgaagatttc aagattaagt ctgaaaccat tttgtacgtc aacgaccagt
     2101 tggaagataa cccacacagt ttgcgtcccg gcatggagtt aaccattcct ccggcagatg
     2161 gtttgtatta cacctggaaa gaaggcgata catttgaatc tgtggcggaa gaatataacg
     2221 ccaatgctga tgacattatc aacttcacag gtaatcagat cgacctgacc gaccccaaag
     2281 tagaagttgg cacccaaatt tttatccctg gtggttctcg tgagttggtg aactgggcag
     2341 cgcaattgcc taccaatgca cgcggagtga acaccggcac aggcggtggc aatgccgcca
     2401 atgtgtgtgg cggtggacct gtagcaagcg gctttggttg gcccgccgat gaccacaccc
     2461 tctctggaaa tgcttacgga cctggtcacc ttggcattga catcagcgcc cctgaaggtg
     2521 ccaatgtgta tgccgctggc actggtgttg taaccatggc ggctggcggt tggaattacg
     2581 gttatggcaa cgtagtgcag atcgatcacg gcaatggtta cgtgacggtt tatgcccact
     2641 taagctccat tctcgtcagt caatgtcaaa ccgtggggca aggctcggtg atcgggctgg
     2701 caggcaacac gggtaactcc ttcggcgcgc acttgcactt tgaaattcgt gtcggtggtt
     2761 cgaatatcaa cccgtacgat attgttcaat aaaatagaga cctgacagtt tcaaagcctg
     2821 tcaggtcttt taatttccat gaacgaatcc tctctcaaaa aacgacttgc aaaactaaac
     2881 ctcggcggct tgcgctattt tgaatcgctg ggctctacta atgacgaagc tcttgcatgg
     2941 gcagctcgtg gcgcggatga tttctctctc gtcgtggcag atgaacaaac tcaagggcgc
     3001 gggcggttag acaggaaatg gttcacgcct aaaggcagtg cgattgcgat gagtctcatc
     3061 ctacgcccta ctgcggtgat gcgctcgcat ctctcgcgga ccgtgggctt ggcagctctc
     3121 tcattggcag atgcttgtct caagctgggt ctgtctccca agatcaagtg gcccaatgat
     3181 attctgctcg atagcaaaaa gactgcaggc atccttgttg aaatggtgtg ggctggcgaa
     3241 gatgccgatt cattggtaat tgggatggga attaacgtcc ataagtctgc agtgccgcca
     3301 agagagacgc tccaattccc tgcaaccagt ttggaagata tgctgggaaa cgctccgaag
     3361 cgagaagata tcatctttca gattttgagc aatgtgcaac gctggcgtga acagatcggc
     3421 acagagcggt tcattcaggc ttgggaggaa aaattagcct ttcgcggaga ggcaattcaa
     3481 atccacgctg gaggcgcaga gtctcatcag ggtgtgttgg atggtttgga gtctgatggt
     3541 agtttgcgca tctgtgatga acataacaaa tctgttattg tgcgctttgg ggatgtgagt
     3601 ctgcgcccga ctgcatgata tacttttaaa aaataatcag aggttcccat gtttgatgat
     3661 cttcgaaatg atgccgcaaa acaatatgaa gacgaagtac aggcagaata tcagccagct
     3721 tcagtcactt catcgggtgc accgcgccaa cgttcaacca agttcctcgg catgacatcc
     3781 atacagcgtt ttatacttgt gttcttattg atgaccgcaa cctgtgtgat tggttttttg
     3841 tgcctttttg cctttggaaa aatcgctttc taagtcgcaa cgcatttaac aatagggtgt
     3901 ttagtatctg catagagtga tagcatgcaa atactaaaca ccctcaattt ttttacaagc
     3961 aaaaatcttt tcaaaagtta taaacggcta ggattctggc aaacgatcat tcttttttcg
     4021 ggggttgctt tcgtttcagt aacaggcatt atcgtcccta ccaacatgct cttgggcgga
     4081 agtatcagaa caggcatggt tatcaacttc attgtttgta caaccttcat gccgtaccac
     4141 cttcaccaaa tccttagcct catggtggag ctagacactt taagaatgaa catatacgac
     4201 aagtcgattc gggatgagtt gacgaaggca tataaccgcc gctacttttt tgaagcgtct
     4261 aaggctatcg agaacaacga agccttaatt ccccaaaata catctctgct attaattgat
     4321 attgatgatt tcaaattgtt aaatgacacc tacgggcacg atattggcga ccttgcgtta
     4381 aagacgctca cagaagaaag ctcacgtctg ttgcgtacta cagatgtctt tgcacggtac
     4441 ggtggcgatg aatttatctg tttgttgccc aacacaaagc aatctcaagc ctatgagata
     4501 gcaacccgcc tgctcgaaag cgcgaacaaa catcctttct acaaaaagga tagaaacatt
     4561 tttctccggc ttagtattgg gattgccaca acaacaagcg agaccaaact taatgaccta
     4621 atttctttag ccgataaagc tttatataaa tcaaaaaaaa acggaaagaa tactgtagaa
     4681 gccttataaa aaagacctag tgagaaatca ctaggtcttt tttatcgtta taccaacttc
     4741 ggctgtggtt cagcttcttg ctcttcgatt tgagcaccag cctttttcag atcatcggct
     4801 gagatgcgtg ccaccgacgc cacgctgtca ccttcttgtg gcttgatgag atgcacgccg
     4861 cgggttgccc gcccagattg tttcacatct ttaaccttca aacgaatcgc tacaccattg
     4921 gcggtcataa tggtcaggtc gtcatctttg ttgaccacac gtgctgctgc aatcttgccg
     4981 atctcttgga gcgccttctg gtcaatggtg gaaataccgc ccgtagcacg tcctttgggt
     5041 gtatattctt tcaaaggtgt ctgcttccca aatccatttg tggtcacaac cagcaatgcg
     5101 ccttctttat ccaccacatc catgctagtg acggcatctt ccttgccaag gcgaatgcct
     5161 tgaacacccg cggcttgacg ccccatcgca cggactttat cttcactgaa tctcaaggct
     5221 tgtccatttt cggtgacgat gatgatctca tcttttccag aggtgaggcg aacccagccc
     5281 agagtgtccc cttcatctaa gttcatggca atcaagccag aggggcgaac tgaggcgaat
     5341 tcttccattg ttacacgctt gatacgccca cgtccggtca tcatgatgca gaactgggtc
     5401 ggcaagaaat cgcggaccga cattgccgct gtcacctttt cgttcggtcc cagcgcaagg
     5461 atgttgacca acggaatacc cttctgagca cgatcgaggt ctgggatttg atacaccttt
     5521 tcggagtaca ccttaccctt atcggtaaag aacagcatcg tgtcgaggct gcgagcaggg
     5581 ataagcatta caacttcgtc ttcgtctttg gttgtgtgcc cctttacacc acgtccacca
     5641 cggctttgct ggcggaaggc agttgccgcc acacgtttga tgtatccacg ctcggtaaga
     5701 ctgatgagca ccgattcatc gggcacaagg tcttcttcgt gaatatcttc gcgagcttcg
     5761 gcagaaattt tggtacggcg gtcatcgcca tatttttctg ccacatcggt catatcgtct
     5821 ttgataagag cgaggatctt atgcgggttg gcaagcaagt cttccaaata tttgatggtt
     5881 tcagaaacct gcttgtgttc ttcttcgatc ttccagcgtt cgagagctgc aaggcgacgt
     5941 aactgcatgt cgagaatcgc ctgtgcttga atttcgctca acttaaagcg ggttatcaac
     6001 gcagacttgg ctttgtctgc atcttctgct tcacgaatga ccttaataat ggcgtcaatg
     6061 ttattgagcg caataagata gccatccaaa atatgcaagc gggctttggc tttggcgagg
     6121 tcaaactgtg agcggcgggt gatgacaatc tgtcgatgtt caatgaagat ctgcaacaga
     6181 cgtttgagcg gcaaagtacg cggctcccca tcgaccaatg ccagcatttg caccccaaac
     6241 gtggactgca aagctgtgta tttataaagc tggttcaaaa ccttcttagg ctgagcacca
     6301 cgcttaagct cgatcacaat gctcatacca cgcttgtccg attcatcgcg caggtctgaa
     6361 atttggtcaa tcttatcctc gcggacgagt tcagcaatgc gttcgatcag gctggattta
     6421 ttgacctgat acggaatttc ggtgatgtta atctgataac gcccggcttt ggtctcttca
     6481 atgtgggcaa tgccgcgcat cacaatgcgt ccgcgcccgg tgccatacgc cgagacaatc
     6541 ccttccgtcc ccacgatcat gccgccggtt gggaagtctg gtccttgcac gaatttcatc
     6601 aaatcgtcga cggtaatctc atcggcttta tcgtagttgt cgataatgta attgatcgca
     6661 ctggtcaact cgcgcaggtt ttgtggcgga atattggtcg ccatacccac agcaatacct
     6721 gaagcgccat tcaacaacaa gttcggtaag cgagcaggga gaacaagcgg ttcttgcaaa
     6781 gaatcatcga agttaggacc ataatccacg gtctcttttt cgaggtcttg caacatttct
     6841 tctgccatct tttgcaggcg ggcttcggta taacgcatcg cagccggcgc atctccatcc
     6901 accgaaccga agttaccctg accatccacc agcatgtaac gcatcgagaa atcttgcgcc
     6961 atacgcgcca tcgactcata cacagccgag tcaccatgcg ggtgatattt acccagcact
     7021 tcacccacaa tacgcgcgct tttcttataa gatgagttcg agcgcaagcc caattcatgc
     7081 atcgcataca aaatgcgacg atgcaccggc ttcataccgt cacgggcatc gggcaaggca
     7141 cgcgccacga tcacgctcat tgcgtaatcg aggtatgccg agcgcatctg ggcatcaata
     7201 tctacagact cgacatttcc agcgatcata ttctcttcag ccataaatta tcctcttgag
     7261 ggcaaaaacg agcgaatttc cgcctttttc acaaaaaaat gacataaaaa ggacacgaag
     7321 acgggaagaa cttcgtgtca cattagatga agaaagcgta attttatagt gcaattatac
     7381 cacccctatc cgctttttgc cttgcagatt aagcatccag cgtagggcaa gcccaatggg
     7441 ataaaccccg cctcctcccc cgcccgagcc gggttctatc catcaaaaaa gacccgcagc
     7501 tgcgagtctt tgtgtgccga aggagggact tgaaccctca tgagattgct ctcactacgc
     7561 cctgaacgta gcgcgtctgc caattccgcc acttcggcat atcatctgaa tggcgggatg
     7621 tattttatcc caaacgggtg gaatgtcaat aagagggtgt tgatgttcaa ctttatgtga
     7681 acaatatcac agcacaaaca taagatgatg acgttgtatt tagtagtgaa aaccccactg
     7741 tggtattgga actatttatg ataaaagtgc gttaatagaa tatacctttc aagagtgtat
     7801 tgtcaaacca ttacagtact gaaaggggag tatctttcta aaaagagaat cgttatattt
     7861 gatatagaaa aagaaaggag ttgcacaatg ttcgtttttg ccgcagtggt tgttgtacta
     7921 ctgctcgccc tggcaagctc tgatatgttc ctcgcgcaat acagccagga tgaactcagc
     7981 aacatgggag ttcaagagca atgatggatg accaccccat cccctagccc aacgcatttg
     8041 atcgaacgcg ttgggctttt tcttttttaa gcggggaaat agttcaagcg agcgagaata
     8101 tcgctggctt ccattaagag ataatccaca attagattta ttgaatagat gtaggcgtct
     8161 aaaataagtg acaggcgaga tattaatgtt acatatgtat ttgggaccca accgctattt
     8221 caaaggggtg tgacttccca actccctacc gctcggtagg gaaacaaagt ttgggatata
     8281 atacgcgcac gagagaatgt agttcgaaac agcatctatc caacgccctc gaatcacgct
     8341 tccacaagga tcgcccatgc agatcactcc gcttcacacc agtattgaaa agcttattga
     8401 gttgaaaata ggtgttgtcg tgatggaaag cccaaatggc ttcccattac acgagaccaa
     8461 catctatttg gttgctccaa gcggggacgt tgtttggaag gcagaaaaac cttcagccaa
     8521 tgtgcttttc accaaaatga agctaaatga agatggcagt atttccactt tcacgaacag
     8581 tggacagttt tgcgacatta acggcgagaa cggcaaggta attagtagtt ccaatttcag
     8641 ctaatagcag gaacaagtgc ggatgcccct ccatttcaca aaactagcga accctatccc
     8701 ggaagaggtc tattctgctt ttaccaaatg ggaaaatgat cctgcacttg tccaccttac
     8761 tcgacccaac caaactcagg cagatatcga cagaaaaagc gaagtagatt cgtttgaatt
     8821 ggacgcacga atttcttacc aaatgaccta cctaatttat ttggatagtc aactggtagg
     8881 tgaaatgagt taccaagtag acccgcagca tctctacaaa aaagagaccg gcaccgcatg
     8941 gatcagcatc accataggtg aagaatctgc tcgaggtaag ggaattggca aactggcaat
     9001 gcaatatctg gaagaagaaa ttcataacgc aggttacaaa cgtattgaat taggcgtttt
     9061 tgaattcaat gaacctgctc aaaagctata tcaaaaaatg ggatataccg aaatcggcag
     9121 aattcccaac ttcacgttct ggcaaaacaa aatgtggacg gacattcgca tggagaaata
     9181 catctgatga tcaaagggtt ccttaacctg ccgtggtttg tttgggctgg gctcgctctg
     9241 gttgttgcgc ttatttatac cttttgggtt cctcaaaaaa tcgcgccaga tataagcggg
     9301 attcgcctgt taatcgtccg ctgggcacac cctctaacat ggtacttgct cgccctcaat
     9361 ttcatcctgc gcggtatttc gccggaatgg ggtggtatcg ccaatatggt tgccgctgca
     9421 ggtggattga tgtatatgtt gtttatgctt atgacctttg tgatcaaata atatggaaaa
     9481 accacctttt agcttcgtcc ttatctttct tttatttagt tttttgttta tttcgaacag
     9541 ctataaactt tggttcaaaa cggatgagta ttacaaagac ctctacgcca gcctcactaa
     9601 tgagcggatt ccctttcctt ttaagagttt ctttctagca cggctggaga aaaaacaaag
     9661 ctggctattc tggcaaaagg cgtttagttt gttgggcatt gtggcagttg tcggtatgga
     9721 tgttttggta gtcatggcgt atttgggata agaaattaac cacaaagtgt cacaaaggaa
     9781 agacactttg tgacccgtcg tggtaaagaa ttgaattcat atcaaggagc ttaacttgaa
     9841 aatcatcgca tgtatcaagc aagtccccga ctcggaggcg aaggtaaa
//
LOCUS       DAOQNJ010000026         9729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3328_length_9729_cov_8.043519, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000026 DAOQNJ010000000
VERSION     DAOQNJ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9729
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_9729_cov_8.043519"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLG52_01525"
     CDS             complement(<1..482)
                     /locus_tag="PLG52_01525"
                     /inference="COORDINATES: protein motif:HMM:NF017365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HNB40146.1"
                     /translation="MRLTNMITAVDLHACGEPGRVITGGVLDVPGKTMFEKMKYMEKN
                     LDDLRLRMLREPRGYPALCCNVIMPPTHPNAAAGFIIMEQTEYPPMSGTNTICVATTL
                     LETGMIPMQEPVTEFMLEAPAGLVGIRAECKDGKVTQVTFKNVPAFAVHLDLHVEVPT
                     L"
     gene            561..986
                     /locus_tag="PLG52_01530"
     CDS             561..986
                     /locus_tag="PLG52_01530"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40147.1"
                     /translation="MDYTRDQFTISNDTSRLDVDEICRFLSRSYWANQRERTVIEKSL
                     QGSLNFGVYDGDRQIGFARVVTDRATFAYLCDVFIHEDYRGKSIGKWMMECIMAHPDL
                     QGLRRWLLATYDAHGLYSQYGFTPLSDPTVMMEIVDRTK"
     gene            1072..3039
                     /locus_tag="PLG52_01535"
     CDS             1072..3039
                     /locus_tag="PLG52_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB40148.1"
                     /translation="MLEELFSKPVGFFLIVMAIILITPLISERLRLPGIIGIIIGGML
                     IGPHGFGLLEDNDRIQFLSTIGLVYLMFSAGLEVDINQFMKVRARALVFGIITFTFPQ
                     LMGMGLGYILGLNWLGMILLGSAFASHTLIAFPILTKLGVTRNEAVAVTTGATVLTDI
                     GAFIVLAVVLGADKGGLSFGYFAQLFVLLAVFTALIIFGLPRFGKFVFQKLTGRAVEF
                     QFVIVALFLAAFFAEVIGVHEVVGAFLAGLAVNSMLPRHSPVAGHVLFIGESFFIPVF
                     LLYSGLITDPLTFLKSPQTIIVAVGVTIVAYVSKLIAAWLTARIYKYTRSEFWTVYGL
                     SHAQAAVTIPTLVIGLETGLFDATLFNAAILMILLTSITSPLIVQRFAPDLQTASTDD
                     EQTPLFGRILVPVSEAHHSEGLVALSSLLARSSKGKVLAVSVAQDKGPNDNSSLMLHK
                     ELLGKVSANLHDPEAELELIPRLAATHAQGILHTANEENASLIVMGWRGKRTFRESVL
                     GSVLDEVIWGSDTPVVAGKLSLPLNSMQRVIFIVPAKAVPPIALRRMLEANLAIAKAL
                     NVPLLVRADKSYVQTLEALFVAISPEQEYKLEVLIDQLKPERLEHEAVSDFIILPGFG
                     SRKRVQDTLGNTPEQIAKYFDGNLALLHFDK"
     gene            complement(3105..4169)
                     /locus_tag="PLG52_01540"
     CDS             complement(3105..4169)
                     /locus_tag="PLG52_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB40149.1"
                     /translation="MKKTLVVFLAFLVAITACTEATPMPTHTPVPVTDTPTQTSTLEP
                     TATFTPAPTATPVPHPMSIIALRNQEYPGSEITVVKELDRGINYRRYYVYYLSEGLKI
                     YALLTIPNGEKPEEGFPAIVFNHGYIPPDVYKTTERYIAYVDQLARAGYVVFRIDYRG
                     HDQSEGEATGAYGDPGYQVDVMNALASLKQHPDVNPEKIGMWGHSMGGYLTLRAMVIS
                     NDVKAGVIWAGVVASYSDLLYNWRRTGAFTPSPSSTGRGWRSAWIEQYGTPEQNPAFW
                     NSISATSYLTDLSGPLELHHGTADEDVPVEFSIRLAELGRQSGQIVNLYTYEGDNHNI
                     SNYFTQAMNQTIAFFDIYLK"
     gene            complement(4277..5212)
                     /gene="mmuM"
                     /locus_tag="PLG52_01545"
     CDS             complement(4277..5212)
                     /gene="mmuM"
                     /locus_tag="PLG52_01545"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HNB40150.1"
                     /translation="MNVLETILNNQRIAIIDGAMATELEARGCDLNDDLWSARVLLEQ
                     PELIRAVHLDYFNAGADIAITASYQATVDGFAKRGLSREQALDLMKKSVLLAQEARDE
                     FWAKEENRIGRIRPLIAGSVGPYGAYLADGSEYRGDYNLSEDELIAFHRPRVEALIAS
                     GADLLACETIPCGIEARALIRLLAEIPNASAWFTFTAKDGEHISNGERIADIAAFLDA
                     KPQAAAIGINCTSPLYIPALVREIKKVTSKPIIVYPNSGEYYSAVTNSWHGETSSDKF
                     GLQSQEWFENGATLIGGCCRTTPGHIHEVSNWARK"
     gene            complement(5209..>5669)
                     /locus_tag="PLG52_01550"
     CDS             complement(5209..>5669)
                     /locus_tag="PLG52_01550"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNB40151.1"
                     /translation="AVIGLTIADEGIPPTAEGRLAAAAKVIERATKIGIPIEDIIIDP
                     LVMTVGHDSKAATVTLKAIELIKKEFDVNMSLGASNVSFGLPDRHSVNAAMLSIAMLT
                     GATTSITDPIKLGSTIRATDLLMGRDPNSMRYLKYFRATEKLREAEAAAK"
     assembly_gap    5670..5769
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5770..6236)
                     /locus_tag="PLG52_01555"
     CDS             complement(<5770..6236)
                     /locus_tag="PLG52_01555"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNB40152.1"
                     /translation="MHTILKSEKKEVTIGFDKPFVIIGEKLNPTGIKKLGQALVERNM
                     DYVKHLAKRQVDWGADVLDVNVGHPQIEEAEVMPLVVEAVQSVTDVPLCIDSNEPKIL
                     EAGLNAIKGGKPLVNSVNGEEKQLSTVLPIVKARGAAVIGLTIADEGIPPTAEG"
     gene            complement(6282..7439)
                     /locus_tag="PLG52_01560"
     CDS             complement(6282..7439)
                     /locus_tag="PLG52_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNB40153.1"
                     /translation="MIKQAHAISEDLIEWRRDFHMHPEIGFDLHRTAGIVADELEKMG
                     YRVKRGVGKTGVVAEIGEGGKLVAIRADMDALPIFEQNETDYISQNQGKMHACGHDSH
                     TAMALGAAKILSQEKLNGRVRFLFQPCEETADEEGKSGAQRMYAEGATEGVDFVIAQH
                     VDPSHPVGTITINEGPSGGGVTSWSATIYGKGGHGAEPHVSNDPFVMLAHVITGINSV
                     VSRRLDPFEPAVISIGAVNGGFAENVIPDKIEMIGTIRFTSLDIEKKIREELTKVFEM
                     VKVLGGDYKLNFLFGGMPIINDKFVSDTIASVGKELLGEGSVLPMDKTLGAEDFPEFL
                     RDAPGAMYMLGVRIEDRPLYELHHPKFDLDERALPIGTAILAETAKRFLNS"
     gene            complement(7527..>7961)
                     /locus_tag="PLG52_01565"
     CDS             complement(7527..>7961)
                     /locus_tag="PLG52_01565"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNB40154.1"
                     /translation="LAGADELSGIGEMEAGVMGSFAQMVLDNELAKSVHRQRQGLSAD
                     AEHLAVEVIGNVMDSNRNYLASKHTLKHLRAGEMALTKLAERNSWDTWEDKLGRKQMA
                     DSATDEAERILREHQVQPLDPAQEAELDKILAAAQRETVKKK"
     assembly_gap    7962..8061
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8062..8693)
                     /locus_tag="PLG52_01570"
     CDS             complement(<8062..8693)
                     /locus_tag="PLG52_01570"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNB40155.1"
                     /translation="MEPLKFLSDADVQAMHEATLQIMSEVGIIWTHKPSLDVLLQAGC
                     KMKDNRVLFPPDLVMDSVAKANKRPKIRGRNGTVNELGGGNLYFHNLGGARDVFDART
                     GTHHTATAEDCVNAVRLLDALPNCNNVTPFFTPPDVENEMMALHMFRHALSNTTKPVQ
                     GPGIQFGHEAKYAVEMAKVIGIEPHEITLSLSPVSPLTMHDIAAQAIMDMA"
     gene            complement(8725..>9729)
                     /locus_tag="PLG52_01575"
     CDS             complement(8725..>9729)
                     /locus_tag="PLG52_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNB40156.1"
                     /translation="DILGWVHSEGAKTSFQFKRGGDTIRAVKVGDGVHAQPQITTDGV
                     VILFEDVNHPTDLWKIDLESGSASQLTKSLDVDLNFQQPEEIWYTSKDGVQVPALLYR
                     GSGENAVLDIHGGPNWHVQFSWNPVLSLMVQHGWTVLVPNYRGSTGYGKKWQNASRYD
                     MGGVDNDDCAAGANYLLKNNLAKKIAVTGRSHGGYLTMTCLTGYPELFVGGSAVVPFL
                     NWIKSHHESREDLKHWNIENMGDPDDNRELWIARSPYFFLDKVTSPVQMICGENDPRC
                     PASDSMDARDKLIELGKEVEFLLYKGEGHSFYKIENIIDAEMKRMGFLERVLTTDRKQ
                     "
BASE COUNT         2156 a         2421 c         2369 g         2583 t
ORIGIN      
        1 agagtgggaa cttccacatg gaggtcgagg tgcacggcaa aggcggggac gttcttgaag
       61 gtgacctgtg tgactttgcc atctttgcac tcggcgcgga tgccgaccag cccagcgggc
      121 gcttcgagca tgaactctgt gaccggctct tgcataggga tcatgcctgt ttcaagcaag
      181 gtggtagcta cgcaaatggt gttcgtgcca gacatgggcg gatactcggt ctgctccatg
      241 atgatgaaac ccgcggcggc attaggatgt gtaggtggca tgatgacgtt gcagcacagg
      301 gcggggtatc cgcgtggttc gcgtaacata cgcaggcgca ggtcgtccag atttttctcc
      361 atgtatttca ttttctcgaa cattgtcttg ccgggcacgt caagcacacc gccggtgatg
      421 acgcgtcctg gttcgccgca ggcgtgcaga tcaacggcgg tgatcatgtt ggtgaggcgc
      481 atgggggttc tccatcaagg gtagaaaatg aattcagacg tagtgtaccg taaatctgcc
      541 cctgcctgat aaaataagcc atggattaca cacgcgacca attcacaatt tcaaacgaca
      601 catcccggtt ggatgtggat gagatttgcc gattcctttc ccgttcttat tgggcaaacc
      661 aacgtgagcg gactgtgatc gagaaatccc ttcaaggttc gctcaatttc ggggtgtacg
      721 atggagacag acagattggt tttgcgcgtg tggtgacaga ccgtgccacg tttgcctact
      781 tgtgcgatgt ctttattcac gaagactatc gtggcaaatc tatcggtaaa tggatgatgg
      841 aatgcattat ggcacaccca gatctgcagg ggcttcgcag gtggttgctg gcaacatacg
      901 acgcgcatgg tttgtacagc cagtatggtt tcaccccgct cagcgacccc acagtcatga
      961 tggagatcgt cgatagaacc aaatgagcct tgagagaatt gggggatggg gaaatttagg
     1021 ctctccagta aaatttttat ttgtacgcca atccaacttt gagggaactc tgtgcttgaa
     1081 gaacttttct ccaaacctgt aggctttttc ttgatcgtca tggcgatcat cctgatcaca
     1141 ccgctgattt cggagcgatt gcgcctgccc ggcattatcg gcatcattat tgggggcatg
     1201 cttatcggtc cgcatggttt cggtctgctt gaagataatg accgcatcca attcctttcg
     1261 accatcggct tggtctacct gatgttcagt gccggtcttg aagtggatat taaccagttc
     1321 atgaaagttc gtgcgcgtgc attagtattt ggcataatca ccttcacttt tccgcaactt
     1381 atgggtatgg gcttgggcta catccttggg ctcaattggc tgggcatgat tctccttggt
     1441 tcggcgtttg cttctcatac cctcattgca tttccgattc tcacgaaact aggtgtcacc
     1501 cgtaatgaag cggtggcggt taccaccggg gcgaccgttc tgaccgatat tggcgctttt
     1561 atcgttctgg ctgttgtcct tggagccgat aaaggtggtt tgagctttgg ctattttgct
     1621 caactctttg tgttgttggc ggtctttacg gcactcatta tttttggttt gccccgcttt
     1681 ggaaaattcg tctttcaaaa acttaccggt cgcgctgttg aatttcaatt cgtgatcgtc
     1741 gccttatttt tggcggcatt ttttgccgaa gtaatcggcg ttcacgaagt ggtaggtgcc
     1801 ttccttgcag gcttggcggt caactcgatg ttgccacgtc atagcccggt ggcagggcat
     1861 gtcctcttca tcggcgagtc atttttcatc cccgtctttt tgctttatag cggtctcatt
     1921 accgacccgc tgaccttcct caaaagtccg cagaccatta ttgttgctgt cggcgtcact
     1981 attgtggcgt atgtttcaaa actaattgct gcctggctca ctgcccgaat ttataaatac
     2041 accagatccg aattctggac cgtctatggc ttgtctcacg cccaagctgc cgtaaccatc
     2101 cctacattgg tcattggttt ggaaactgga cttttcgacg ccaccctctt caatgccgcc
     2161 attttgatga ttctcctcac ctcgatcact tcgcctctta tcgtccagcg cttcgcccct
     2221 gaccttcaaa ctgcttctac cgacgatgag cagacgccac tcttcgggcg gattctcgtc
     2281 cctgtttctg aggcgcatca ttccgaaggg ttggtggctc tttccagctt attagcccgc
     2341 agttcaaagg gcaaggtatt ggcagtgagt gtggcgcaag ataaaggtcc aaatgacaat
     2401 agtagcctga tgttgcataa ggaattgctc ggaaaggtca gtgctaattt acacgacccc
     2461 gaagcagaac ttgaattgat cccacgcctt gcagcaacgc atgcacaagg tattttgcac
     2521 accgcaaacg aagagaatgc ctccctaatc gtcatggggt ggcgcggcaa gcgcaccttc
     2581 cgtgagagtg tgctcggttc tgttctggat gaagttatct ggggttctga tacacccgtg
     2641 gtggctggga agctatcttt gccgctaaat agtatgcagc gcgtaatctt catcgtgcct
     2701 gccaaagctg tcccgccaat cgcgttacgt cgcatgctcg aagccaacct tgccattgcg
     2761 aaggcgctta atgtgccctt gctcgtccgg gcagataaga gctatgtaca gaccctggag
     2821 gcattgttcg tcgccatcag ccctgaacag gaatataagc tcgaagttct gatagatcaa
     2881 cttaagccgg aacgcttgga gcacgaagcc gtcagtgact ttatcatttt gccggggttt
     2941 ggctcccgca agcgcgtgca agataccctt ggaaacaccc ccgagcaaat cgctaaatat
     3001 tttgacggaa atttagcact cctgcatttc gataagtaaa ttatctgatc atggacgatg
     3061 ggcaataaac cgcgatcaac ggtctattgc ccatcgtctt gtttttactt caggtaaata
     3121 tcgaagaatg caatcgtctg attcatggct tgggtaaaat agttcgaaat gttgtgatta
     3181 tcaccttcgt atgtgtacaa attaacgatt tgacctgatt gcctgcccaa ctctgccaac
     3241 cgaatcgaaa actctaccgg cacatcttca tctgcagtgc catgatgcaa ttccaacgga
     3301 ccggatagat cagtaaggta ggatgtcgca gagattgagt tccagaaggc ggggttttgc
     3361 tcgggtgtgc cgtattgttc gatccatgct gatcgccatc cacgcccggt agagctaggt
     3421 gacggggtga aggcacccgt ccggcgccag ttatacaaca ggtctgagta tgaagcgacg
     3481 acccccgccc agatcacacc ggcttttaca tcattcgaga tgaccatggc gcgcagggtt
     3541 aaatatccgc ccatggaatg cccccacata ccgatcttct cagggtttac atctgggtgc
     3601 tgtttgagag aggcgagggc attcatcaca tctacttggt agccggggtc gccataggca
     3661 ccagtggctt ccccttcgga ctgatcgtgc ccgcgataat cgatgcggaa gaccacatac
     3721 cctgcccgag cgagttgatc gacatatgca atatagcgct ctgttgtttt gtatacatct
     3781 ggcggaatgt aaccatgatt gaagacgatg gcagggaacc cttcttcagg tttttcacca
     3841 ttcggaatgg tgagcaaggc gtaaatcttc aacccttctg aaagatagta aacataatat
     3901 cggcgataat taatgccgcg gtctagctct ttgacgacgg tgatttcgct gccgggatat
     3961 tcctgatttc gcaacgcgat gatgctcatg ggatgtggaa cgggcgtggc tgttggagct
     4021 ggtgtgaatg tagctgtggg ctcgagtgtg gaggtttgcg tgggcgtatc cgttactggg
     4081 actggcgtat gggtgggcat gggagtcgct tcggtgcagg ctgtgatcgc caccaaaaac
     4141 gcgaggaata ccactaaagt tttcttcatt ctttctcctt taaatttttc actcaacgtg
     4201 atgtagaaca aaaaagagac ggtccattac cgtctcttta tacatggttt gtatcagtaa
     4261 aagttaagaa agaattttat tttcttgccc aattgctcac ttcgtgaata tgccccggtg
     4321 tggtgcggca acatccgccg atgagggtag caccattctc gaaccattct tgggattgca
     4381 aaccaaattt gtcgctagaa gtttcgccgt gccagctatt ggtcaccgcg ctgtaatact
     4441 cgccagagtt cgggtagaca ataatgggct tgctggtgac cttcttgatt tcacgcacta
     4501 acgcagggat gtataagggc gaggtgcagt tgatgccaat tgctgcggct tggggctttg
     4561 catcgagaaa agcggcaata tctgcgatgc gttcaccgtt gctgatgtgt tcaccgtctt
     4621 tggcagtgaa ggtgaaccag gcagaggcat tcgggatttc agccaggagg cggatcaaag
     4681 ctcgggcttc gatcccgcat gggatggttt cgcaggctaa taggtccgca ccggaggcga
     4741 tgagggcttc cacgcgcggg cgatgaaatg cgatcagctc atcttcactc aggttgtaat
     4801 caccgcgata ttcagaccca tcagcaagat aagcgccata aggtccaacc gaaccggcga
     4861 tcaatggacg gatgcgtcca atgcgatttt cttcctttgc ccaaaattca tcgcgagctt
     4921 cctgcgccaa cagcacagat tttttcatga gatctagcgc ttgctcgcga ctcaagccac
     4981 gtttggcaaa cccatctacc gtggcttgat aactggcagt gatggcaatg tcggcacctg
     5041 cattgaagta atcgagatga accgcacgaa ttaactcagg ctgttcaagc aagacccgtg
     5101 ccgaccaaag atcatcgttc aagtcacagc cgcgggcttc gagttcggtt gccatagcac
     5161 catcaataat cgcgatgcgc tgattgttga gaatagtttc tagtacgttc attttgcagc
     5221 tgcttctgcc tcgcgtaatt tttcggtagc gcggaagtat ttgagatagc gcatcgagtt
     5281 cgggtcgcgc cccataagca agtctgtagc gcggatggtg ctgccgagtt tgatggggtc
     5341 tgtgatagaa gttgttgcgc cggtgagcat agcaatcgaa agcatggcgg cgttgacgga
     5401 gtgtctatct ggcagaccaa acgaaacatt gctcgcgccg aggctcatat tcacatcgaa
     5461 ctctttcttg atcaattcaa tcgccttgag cgttacggtg gcagctttcg agtcgtgccc
     5521 aaccgtcatc accagcgggt caatgatgat gtcttcaatc ggaatgccga tctttgtagc
     5581 gcgttcaatg acctttgcgg cggctgcgag gcgtccttct gcggtaggtg ggatgccttc
     5641 atcggcaatc gtcaagccaa tgactgctgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnc cttctgcggt aggtgggatg ccttcatcgg caatcgtcaa gccaatgact
     5821 gctgcgcctc ttgccttgac gatcggcagc actgtgctaa gttgcttttc ttccccgttg
     5881 acggagttca cgagcggctt gccgcctttg atggcattca aacccgcttc aagaattttc
     5941 ggctcgttcg agtcgatgca gagcggcaca tcggtcacag actgaaccgc ttcgaccacc
     6001 aacggcatga cttccgcttc ttcgatctgc ggatgtccca catttacatc caacacatcc
     6061 gcgccccaat caacctggcg tttggcgagg tgcttgacat aatccatgtt gcgctcaacc
     6121 aaggcttgcc ccagcttttt gatgccggtg gggttgagtt tttcaccaat gatgacgaat
     6181 ggcttgtcga aaccgatcgt gacttctttt ttctctgact tgagaatggt atgcacaggt
     6241 gccctccgaa agtttaatta tgagacgata gagtctctag attatgagtt caaaaagcgt
     6301 tttgctgttt cagccaaaat ggctgtacca atgggcaagg cgcgttcatc gaggtcgaac
     6361 ttggggtgat gtaattcata caaaggacga tcttcgatgc gcacacccag catgtacatg
     6421 gcgccgggag catcacgtag aaattctgga aagtcttccg cacccaaggt cttatccata
     6481 ggcaagacac tgccttctcc tagcagttct ttgccaacgc ttgcaatcgt atcggacaca
     6541 aatttatcgt tgatgattgg catgccgcca aagaggaagt ttaatttgta atcaccgcca
     6601 agcactttaa ccatttcgaa cactttggtc aactcttcgc gaatcttttt ctcaatatcc
     6661 agcgaagtaa agcggatggt tccgatcatt tcgatcttat cggggatgac attttctgcg
     6721 aacccgccat tgaccgcacc aatgctaatg actgctggct caaacgggtc aagcctacga
     6781 gagaccacgg aattgattcc cgtgatcacg tgcgccagca tcacgaacgg atcgttggaa
     6841 acatgcggct cggcaccgtg cccgcccttg ccatagatcg tcgcagacca ggatgtaacc
     6901 ccgcctccgc tcggaccttc attaattgta atggtcccaa ctgggtggct tgggtctaca
     6961 tgctgcgcaa tgacaaagtc cacgccttcg gttgcacctt ctgcgtacat gcgttgtgcg
     7021 ccgcttttgc cttcttcatc ggcggtctct tcgcaaggtt ggaagagaaa acgaacacgt
     7081 ccgttcagct tttcttgaga aaggattttt gccgcaccca atgccatggc agtatgcgag
     7141 tcatgaccac aggcgtgcat tttgccttgg ttttgtgaga tgtaatcggt ctcattctgt
     7201 tcaaagatcg ggagtgcatc catatcggca cgaatggcga ccaacttgcc gccttccccg
     7261 atctctgcta caacaccagt cttgccaacc ccacgtttta cacgatagcc catcttttcc
     7321 agttcatctg ccacaattcc ggcggtgcgg tgcaggtcaa aaccgatttc cgggtgcatg
     7381 tgaaaatcgc gccgccattc gatgaggtct tctgaaattg catgtgcttg tttgatcatt
     7441 ctatattctc ctgtatttaa gtatacaagc aaacttgagc acaaggatac cttctattcc
     7501 ttgcgccaat taacttatat acctgcttac tttttcttta cggtctcacg ttgtgccgcg
     7561 gcaaggattt tatccaactc agcttcttgg gctgggtcga gcggttgtac ctgatgttcg
     7621 cgcagaattc tttcggcttc atcggtggca gagtctgcca tttgtttgcg ccccagtttg
     7681 tcttcccagg tatcccacga attacgttcg gcaagtttgg tcaatgccat ttcgccggcg
     7741 cggaggtgtt tgagcgtatg cttgctggca aggtagttgc ggttggaatc cattacgtta
     7801 ccaattactt ctaccgccaa atgttcggca tctgctgata agccttgacg ttgacgatga
     7861 acactctttg ccaattcatt atcgagcacc atttgcgcga aggaccccat cacaccggct
     7921 tccatttcgc caatgcccga gagttcatcg gctccggcta gnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn ngccatatcc atgatggctt gggcggcaat atcgtgcatg
     8101 gtcagcggac tgaccggcga aagcgaaagc gtgatctcat gcggctcaat accgatgacc
     8161 tttgccattt ccacggcgta ttttgcttca tgcccaaact gaatgccagg accctgcact
     8221 ggtttggtgg tgttcgagag cgcatggcgg aacatatgca atgccatcat ttcattttcc
     8281 acatctggcg gagtaaaaaa tggtgtaacg ttattgcagt ttgggagtgc gtctaacaaa
     8341 cgaaccgcat tcacgcaatc ttctgcggtt gcggtgtgat gtgtgcctgt gcgtgcatca
     8401 aacacatcac gtgcgccgcc gaggttatgg aaatataaat tcccgccgcc caattcattg
     8461 accgtaccat ttcgcccacg aatctttgga cgtttattcg cttttgcaac cgaatccatc
     8521 acgaggtcgg gaggaaacaa aacgcggttg tctttcattt tgcagcctgc ttgtaacaaa
     8581 acatcgaggc tgggtttgtg agtccaaata atgcctactt cgctcataat ctgcaaagtg
     8641 gcttcatgca tggcttgtac atccgcgtca gacaaaaact ttaaaggctc catggttatc
     8701 tcctcggtaa aaatgtaggc gtacctattg cttacggtct gtcgttaata ctctttctaa
     8761 aaatcccata cgtttcattt ctgcatcgat aatattctcg atcttataaa acgaatgtcc
     8821 ttcccctttg tataacagaa attctacttc tttccccagt tctatcaatt tgtctctggc
     8881 atccatagag tcagatgccg gacagcgcgg gtcgttttcg ccacagatca tttgcacagg
     8941 cgatgtgact ttgtctaaaa agaaatatgg agagcgggca atccacaact cacggttatc
     9001 gtcggggtcg cccatgtttt cgatattcca gtgtttaagg tcttcacgtg actcgtgatg
     9061 tgattttatc caatttaaga acggcacaac cgctgagccg cccacaaaca attcggggta
     9121 tcccgtcaaa caagtcatag tgagataccc accatggctt cgaccagtta cagcaatctt
     9181 ctttgctaaa ttatttttca ataaatagtt tgcccccgct gcgcagtcgt cattatccac
     9241 gccgcccata tcgtaacggc tggcgttctg ccattttttg ccataccctg tcgagccacg
     9301 ataatttggc acaagcacag tccaaccatg ttgcaccatc aaactcaaca cagggttcca
     9361 tgaaaactgc acatgccagt tcggtccgcc atgaatatca agcacagcat tttcaccaga
     9421 cccgcggtac aacaatgcag gcacttgcac gccatcctta ctggtgtacc aaatctcttc
     9481 gggctgttga aagttcaagt ctacatctag tgatttggtc aactgtgagg cacttccgct
     9541 ttcaagatcg attttccata aatcggtagg gtgatttaca tcttcaaata ggatgacgac
     9601 accatcggtt gtaatctgcg gttgcgcatg gacaccatca ccaaccttca ctgcacgaat
     9661 ggtatcgcct ccgcgcttaa actggaaact ggtctttgca ccttcgctgt gtacccagcc
     9721 gagaatgtc
//
LOCUS       DAOQNJ010000027         9555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3429_length_9555_cov_8.495474, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000027 DAOQNJ010000000
VERSION     DAOQNJ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3429_length_9555_cov_8.495474"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLG52_01580"
     CDS             <1..594
                     /locus_tag="PLG52_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB40157.1"
                     /translation="KTITAHGIHFDAREMEILKETETWLTHQPRSNMNNGVGTAQIES
                     MLRLGIKVCLGNDGFTNSMWDDWKVAYLLHKAEHRDPRRMGGYEVQQMAIYNNAALAQ
                     NFFPEAPVGQIIPGAAADLIFVDYHPFTPLTDGNLPWHIIFGFQQSMVTSTMVAGKFL
                     MKDRELLTLNEEEIAARAREIAPRIWKKYEEEVAKVM"
     gene            618..1292
                     /gene="npdG"
                     /locus_tag="PLG52_01585"
     CDS             618..1292
                     /gene="npdG"
                     /locus_tag="PLG52_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNB40158.1"
                     /translation="MKEDHILVSVAVLGGTGKEGKGLAYRWARAGYHVLIGSRSEEKA
                     VAAAKEVTEMLGDGVSVAGMNNLQAAKEANIVVLTVPYAAHRETLESVKDALQGKILV
                     DVTVPLVPPKVATVQMPAAGSAAQEAKQILGEGVEVCAAFQNISHEHLLGGEGVECDV
                     LVTGTSKDARANVILLVEAAGLRGWDAGPIENSVVVEGLTSVLIGINKKYGSTHAGIK
                     ITGISK"
     gene            1347..2117
                     /gene="cofE"
                     /locus_tag="PLG52_01590"
     CDS             1347..2117
                     /gene="cofE"
                     /locus_tag="PLG52_01590"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNB40159.1"
                     /translation="MSLTLNTLKNIPLIRQGNNLADILLKSLHESGIALQDNDILVLA
                     QKIVSKTEGRMVNLAKIEPSSAALELAPIIDKDARLIELILQESNEVLRIRKGVIVVE
                     HKLGFVCANAGIDHSNVMGDGKEDEEYVLLLPENPDTSARWLREEILRKTDKTVGIMI
                     IDSHGRAWRNGTVGVCIGLSGIPALVDERGWKDLFGYTLKATIVGVADELAAAASLMM
                     GQAAEGTPVVHVRGFPYPLSESGSLQELIRPKEMDMFR"
     gene            2191..2583
                     /locus_tag="PLG52_01595"
     CDS             2191..2583
                     /locus_tag="PLG52_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNB40160.1"
                     /translation="MPFPENFLDLIQPETKSFLYLATVNAKGVPQVTPVWFDTDGEHI
                     LINTNEGRLKDRNMKKNENVAMVIQDPKSPYRYLGMQGKVVSYTTEGADEHINLLSLR
                     YDEEPWKYREGQKRIIFKIKPTSFDEHN"
     gene            2567..3193
                     /locus_tag="PLG52_01600"
     CDS             2567..3193
                     /locus_tag="PLG52_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNB40161.1"
                     /translation="MTNTIDLHTFLRTRRSIRRFKPDPIPDSVIREILHTATLAPSAH
                     NRQPWRFVVMTDKTSIAEFAKTMAIEFRHVLEQDQLPPEEIQKRLNRSQERISGAPVV
                     IIACVEMTEMEKYPDERRKKAEYLIATQSVANAGMQLLLAAHAEGLGAVWICSPLFAQ
                     SAVQEALRLQPTWEPQAMFLVGKPVEVPAVRERKPIEEISIFIDKKGN"
     gene            3195..4109
                     /gene="cofD"
                     /locus_tag="PLG52_01605"
     CDS             3195..4109
                     /gene="cofD"
                     /locus_tag="PLG52_01605"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HNB40162.1"
                     /translation="MKIVAFAGGVGGAKLAQGLAQTLPPKDLTIIVNTGDDFEHLGLY
                     ICPDLDTVCYTLGGLANPETGWGRAGETWNTIANVEKLGGPAWFRLGDSDIATHLERT
                     RRLKEGKSLSEITKEFCKAWGIEATVLPMTDSPVRTMVDSDEGELAFQEYFVHRQCQP
                     RVKGFRFDGVEVAEPAQGVHEALASADAVIFCPSNPWVSIDPILSVVKEVNKPVFAVS
                     PIIGGKTVKGPAAKMYSELGIEPSAYAVAKHYRTILKGFVLDTTDAAMEKEIQDLGFA
                     TLVTNTLMNQLTDRARLANDVLNFIRSL"
     gene            4112..4720
                     /gene="cofC"
                     /locus_tag="PLG52_01610"
     CDS             4112..4720
                     /gene="cofC"
                     /locus_tag="PLG52_01610"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNB40163.1"
                     /translation="MSLWAIVPVKPLRRGKSRLAGALTEEERAALNQELLERTLKTLT
                     SLKELDKVLVVSRDSHALTIARNHGAKTVQEDGQPHLNTALTRASVIAKVHATQGVLV
                     LPVDLPLLTTDDVLALIDRATKPPVVVIAPDRHHKGTNALLMVPAGQIEYDFGEGSFK
                     RHSERALQSGARLEVVELPSLGLDLDDPEDLELIRKLEAQKV"
     gene            4835..5836
                     /locus_tag="PLG52_01615"
     CDS             4835..5836
                     /locus_tag="PLG52_01615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNB40164.1"
                     /translation="MTKQRVALYLQDSHDLRDGLDYAKYAEEKGFEAVWQAESRLVRD
                     AIVPMAGYAAVTNKIKVGSGVINNWTRNIGLLAATFLTLDDLAPNRVICGLGAWWDPL
                     AANVGIDRKKPLTAMKETVQVMKKLLNMERVTFDGEFIHVKGIELDVVYGRREPRNIP
                     IMIGATGDKMMELTGEIADGAVLNYCVAPEYNDNAMELLNNGLKKSGRKMEDFDRPQL
                     IVCSVDEDHDKAINYSKMLLCQYIAQQPHIAKASNVSDDVVQQIQSILGWPATKEQVM
                     KAKDLVPDELVHKITASGTPTEAKAKVEEYKKRGCTCPILYPVGGNFKLLIDTFAQN"
     gene            5940..6947
                     /locus_tag="PLG52_01620"
     CDS             5940..6947
                     /locus_tag="PLG52_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB40165.1"
                     /translation="MRLKIIGFFLVLGVLIAACAPTSAPATEVAETEAVSQVQTEAAA
                     ENVGTLKIAVLPIIDTLPIYVAEAEGLFAKHGVTVELIPVASAPERDQLLAAGQADGT
                     INETLAVMLFNKDAVQMQVVRYALRPGEGYPHFRILASNQSGITLSTELKGVEIGVSQ
                     GTIIEYVTERLLKTQGLAPDEIKTIAVPKIPDRMALLGSGELSAAVLPDPLASLAMSQ
                     GAVNVLDDSMNPEYGFSVYSFRKDVIDANPEAVKAFLAAIEEARSLINPNPSKYTSLL
                     SDKGLIPPPLLETFRVPPFPQSEVPTEAEWNDGLAWLKEKGILSVDVSYADSVNSSLL
                     P"
     gene            6993..7766
                     /locus_tag="PLG52_01625"
     CDS             6993..7766
                     /locus_tag="PLG52_01625"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40166.1"
                     /translation="MISIQSLTFEYNHHSPLFQNFNWTVSCGETWSILGPSGCGKTTL
                     LYLLAGLKFPTSGEVSIHGEKIQRPRPHSGLILQDYGLLPWSTVRENVALGIKVRTFY
                     GADGKHTPNNFTPKDNIESWIERLGLSEVADKFPSQLSGGQRQRTAIARTLALEPDLL
                     LMDEPFSSLDAVTREDLQNLTLSLCAEQNITLVIVTHSIEEAAVLGKKILLLSNPPNQ
                     TVQVFDNPNAGTTGFRHTPEFHSMCEHLWREMQKGNVSL"
     gene            7763..8503
                     /locus_tag="PLG52_01630"
     CDS             7763..8503
                     /locus_tag="PLG52_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40167.1"
                     /translation="MKRRDVLFAALAILLVWQLAAMIVKMPILPSPLTVCMVFFKEMQ
                     NGLYIHFAVSLWRVTAGMLLSVLVAAPAGLLIGGSKSLNRLLSPIIYLLYPIPKVVFV
                     PVIILFLGIGDTSKIVIMFLILFFQIIVLVRDQASGIAPQLLQSLQSLGAGRRALFRF
                     VYLPASLPAILTALRQSIGTAVAVLYITELSATKYGLGYYIYYKGSTLLDYPSMYAGV
                     IAMSLLGLGMYFTVDWLERKWCPWKFVS"
     gene            complement(8496..9395)
                     /locus_tag="PLG52_01635"
     CDS             complement(8496..9395)
                     /locus_tag="PLG52_01635"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNB40168.1"
                     /translation="MTETNKPNMFQIWWLAIRPRTLPAAAAGVAMGSALAWHDGSFQL
                     LPALAALLVALLLQVGSNVANDVFDFERGADTAERLGPVRVTQAGYLTPSQVKTGMVV
                     IFAFAAVLGLYLATLRGWTVIWLGAAAILSAVAYTGGPYPLGYHGLGDVFVFLFFGLA
                     AVAGTYFVQVGSVSPAAWWMAVPVGLIVTAILVVNNLRDLENDRKAGKRTLAVRFGEE
                     GAKAEYLLLMLIAYLIVPVLVWMNIIPIGALLAWLSLPIAIRTLRTVFTEKGKPLNVA
                     LAGTGQTAFLFSLFFWVGLLFSN"
BASE COUNT         2647 a         2282 c         2321 g         2305 t
ORIGIN      
        1 aaaaccatta cagcacatgg tatccacttt gatgcacgcg aaatggaaat cctaaaagaa
       61 accgaaacct ggcttaccca ccagccccgc tccaatatga ataacggtgt gggcacagct
      121 caaatcgaat ctatgctccg gcttggcata aaggtctgtt tgggcaatga tggcttcacc
      181 aattccatgt gggacgattg gaaagtggca tatctcctgc ataaagccga acatcgcgat
      241 ccacgccgca tgggcgggta tgaagtgcaa cagatggcga tctacaacaa tgctgcactt
      301 gctcagaact tcttccccga agcacctgtt gggcaaatca tccccggtgc tgccgcagac
      361 ttgatctttg tggattatca ccccttcacc ccgctaacag atggcaacct gccatggcac
      421 atcatctttg gttttcaaca aagtatggtg actagcacta tggtagctgg aaaatttttg
      481 atgaaagatc gtgagttgct cactctcaac gaagaagaaa ttgcagcgcg cgcaagggaa
      541 atcgctccgc gcatttggaa aaagtacgaa gaagaagtcg ccaaggtcat gtagagaacg
      601 tccctttagg agaactcatg aaagaagatc acattttagt ttcagttgct gtcttgggcg
      661 gcacaggcaa agaaggtaaa gggctagcct atcgttgggc tcgcgccggg tatcatgtat
      721 tgattggttc tcgttcagag gaaaaagcgg ttgcggcagc caaagaagtg acagaaatgc
      781 tcggcgatgg agtctctgtc gctggaatga acaatcttca ggcggcgaaa gaagcgaaca
      841 tcgtcgtgtt gaccgttcca tatgcggcac atcgcgaaac actggaatcc gtcaaagatg
      901 cattgcaagg caaaatcctt gttgatgtga cggtacctct cgtcccgccg aaagtggcta
      961 cagtgcaaat gccagcagcg ggttctgcgg cacaggaagc caaacaaatt ctcggcgaag
     1021 gcgtggaagt ttgtgcagcg ttccagaata tttcgcatga gcacttgctc ggcggcgaag
     1081 gcgtggaatg cgatgtactt gttacgggta ccagtaagga tgcgcgtgca aatgtcatcc
     1141 tgttagtgga agcggctggt ctacgtggct gggacgcagg accgattgaa aattcggttg
     1201 tagttgaagg tctcaccagc gttttgatcg gtatcaataa aaagtacggt tctactcatg
     1261 ccggaataaa gatcaccgga atctctaaat aagcctccgt gcgtaatgcg tacatttgac
     1321 gtgttgcgca ttacaaaacg cgtatcatgt ctctcactct aaacacactc aaaaatattc
     1381 ccctcatccg ccaaggtaac aacctggcgg atattcttct taagtcattg cacgaatcag
     1441 gcattgctct gcaagataat gacattctcg tgcttgcaca gaagatcgtt agcaaaaccg
     1501 aaggacgcat ggtcaacctt gcaaagatcg aaccttcatc tgcggcactt gaacttgccc
     1561 cgatcattga taaagatgct cgcctgatcg aattgatctt gcaagaaagc aatgaagttt
     1621 tacgtattcg caaaggggtg atcgttgttg aacataaact tgggtttgta tgtgccaacg
     1681 ccggcattga ccattccaat gtcatgggcg atggcaaaga agatgaagaa tatgtactgc
     1741 ttctgccaga aaacccagat acatctgcaa gatggttgcg cgaagaaata ctacgcaaaa
     1801 cagataaaac cgtcggcatt atgatcatcg actcgcatgg gcgtgcctgg cgcaatggaa
     1861 cggtaggtgt ttgtatcggg ctcagcggaa ttccagccct tgtcgatgaa cgcggatgga
     1921 aagacctctt tgggtacacg ctcaaagcaa ctattgttgg tgtggcagat gaacttgccg
     1981 ccgccgcttc cctaatgatg ggtcaagccg cagaaggtac gccggttgta catgtgcgcg
     2041 ggtttccgta cccgctcagc gaaagcggct cacttcaaga gcttattcgc cccaaagaaa
     2101 tggatatgtt ccgataagct ctaacaggaa tcttatgcgc ccttgatata aaaggtacgc
     2161 aaggctcgaa taaaattcct ggagaataca atgcccttcc ctgagaactt cctcgacctc
     2221 attcaacctg agaccaaatc ttttctttat cttgccaccg tcaatgcaaa gggggttcca
     2281 caagtgaccc cggtatggtt cgatacagat ggtgaacata ttcttattaa cacaaacgaa
     2341 ggtcgcctca aagatcgcaa tatgaaaaag aacgaaaacg tggcaatggt cattcaagac
     2401 cccaaaagcc cgtatcgtta tctcggcatg caaggcaaag tggtttctta cacaactgaa
     2461 ggcgcagatg agcatatcaa cttgctttct ttgcgttacg atgaagaacc atggaaatac
     2521 cgcgaggggc aaaagcggat tatttttaag atcaagccga cttcctttga cgaacacaat
     2581 tgatctgcac acctttctgc ggacaaggcg ctcgattcgc cgcttcaaac cggacccgat
     2641 tcctgattca gtcatccggg aaattctcca cactgccact ctcgctccat ccgcgcataa
     2701 ccgtcagcct tggcgttttg tggtgatgac agataaaacg tctatcgctg agtttgcaaa
     2761 gacgatggca atagagttca ggcatgtgct tgagcaagat caacttccgc ccgaagagat
     2821 tcaaaaacgc ctaaatcgct cccaagaaag aatcagcggc gcaccagtcg tcattatcgc
     2881 gtgtgttgaa atgaccgaaa tggaaaaata cccagatgaa cgccgtaaga aagccgaata
     2941 tctgatcgca acgcaatcag ttgccaatgc cggcatgcag cttttactgg cagcgcatgc
     3001 agaaggcttg ggcgccgtat ggatatgcag cccattattt gctcaatctg ctgtgcaaga
     3061 agcattaaga cttcaaccca catgggaacc tcaagctatg ttcctggtcg gtaaaccagt
     3121 agaggttccg gcggtgcgag aacgcaaacc gatcgaagaa atttcgatct tcattgataa
     3181 aaagggaaac taaattgaaa atcgtagcat ttgcaggcgg ggttggcgga gcaaaactgg
     3241 cacaaggact cgctcaaacc cttccaccaa aagacttaac cattatcgtc aacactggtg
     3301 atgactttga acatttgggg ctttatatct gcccagacct tgataccgtt tgttacacct
     3361 tgggcgggct tgccaacccc gagaccggtt ggggtcgggc tggcgagacg tggaacacca
     3421 tagctaacgt cgaaaagctt ggcggaccgg catggtttcg ccttggcgac tcggatattg
     3481 caacccatct tgagcgcacg cgacgactaa aagaagggaa gtctctctca gagatcacaa
     3541 aagaattttg caaagcttgg ggcattgaag cgacagtgtt gcccatgacg gattcgcctg
     3601 tgcgaaccat ggtcgactct gatgaaggcg aattggcatt tcaagaatat ttcgtccaca
     3661 gacaatgcca accgagagtg aaaggcttca ggttcgacgg agtcgaagtc gctgaaccag
     3721 ctcagggtgt gcatgaggcg cttgcgtctg cagatgcggt cattttctgc ccatccaatc
     3781 cgtgggtcag tattgatcct attctgagcg ttgtcaaaga agtgaataaa ccggtttttg
     3841 cggtttcccc catcattggt ggaaagacgg tgaaaggacc ggcagcaaag atgtatagtg
     3901 aacttggtat tgaaccgtca gcttatgcgg ttgccaaaca ttatcgcacc atcttaaaag
     3961 gatttgtgtt agacactaca gatgctgcca tggaaaaaga aattcaagac cttggctttg
     4021 caactcttgt cacaaacaca cttatgaatc agcttacaga tcgcgcacga ctggcgaacg
     4081 atgtgctaaa ctttatccgg agtttataaa aatgtcactt tgggcaattg tgcctgtaaa
     4141 accattgcgc cgcggaaagt cccgccttgc cggggcatta actgaagaag aacgcgcggc
     4201 attgaatcaa gaattactgg aacgcactct caaaaccctt accagtttaa aagaattaga
     4261 caaggtactg gtcgttagcc gcgactcaca tgcattgacc attgcacgta atcatggcgc
     4321 taaaaccgtt caggaagatg gtcaaccgca tttgaacaca gcgttaacgc gtgcatctgt
     4381 gatcgcaaag gttcatgcaa ctcaaggcgt gttggttttg cccgtagatc ttcccctgct
     4441 tacaactgat gatgttttag ctttgatcga ccgagctacc aaaccacccg ttgtggtcat
     4501 tgcaccggat agacatcata aagggacaaa cgctttgctc atggttccgg ctggacagat
     4561 cgaatatgac tttggtgaag ggtcttttaa aaggcatagc gagcgagcgc ttcaatctgg
     4621 tgcaaggttg gaagtggttg aacttccatc gcttgggtta gacttggatg accctgaaga
     4681 cttggaactc attcgcaagt tggaagcaca aaaagtatag acatcagttt tgccacatgc
     4741 tcgcaaaccc tatgaaattt gcgaaacatg tggcaaaatt ttttatattg tcagacaatc
     4801 aagtcgtatc attccacact cgaaggagaa tgtcatgacc aagcaacgtg ttgctctgta
     4861 cttgcaagac tcgcacgacc tacgtgatgg gctcgactat gcaaagtatg ccgaagaaaa
     4921 agggtttgaa gccgtatggc aagcggagag tcgcttagtg cgcgatgcga tcgtgccgat
     4981 ggcagggtat gccgctgtaa ccaacaagat caaagtcggc tcgggtgtga tcaataactg
     5041 gactcgcaat attggtctgt tggctgccac cttcttaact ttggatgatc ttgctcctaa
     5101 ccgggtcatt tgcgggctcg gcgcatggtg ggacccacta gctgccaatg taggcattga
     5161 ccgcaagaag cccttgactg ccatgaaaga aaccgtgcag gtcatgaaga aattgctcaa
     5221 catggaacgt gtcacctttg atggggaatt cattcatgta aaaggtattg aattggacgt
     5281 ggtatatgga cgacgggaac cgcgcaacat tcccatcatg atcggtgcga cgggcgataa
     5341 gatgatggaa ttgaccggcg aaattgcaga cggtgctgtg ctcaattact gcgttgcccc
     5401 tgaatacaat gacaacgcca tggaattgct gaataatggc ttgaagaaat ctggtcgtaa
     5461 gatggaagac tttgaccgtc cgcagttgat cgtctgctca gtagatgaag accacgataa
     5521 agctatcaat tacagcaaga tgctgctctg ccagtatatt gctcaacagc cgcacattgc
     5581 caaagcttca aatgtatccg atgatgtcgt gcaacagatc cagtccattt tggggtggcc
     5641 tgctaccaag gaacaggtga tgaaagccaa agacttggtg cctgatgaac tcgtacacaa
     5701 aatcactgca tctgggacac ctacagaagc taaagccaaa gtggaagaat acaaaaagcg
     5761 cggatgcacc tgccccatcc tctacccggt aggcggaaat ttcaaacttc tgatcgacac
     5821 ttttgcacag aactaaatcc tgacattttg cacctgatat gtatgagccg tgtcaccttg
     5881 acgcggctca ttgcgtgcta tatacccaat aaaaagaaaa acagactcag gagtattcca
     5941 tgcggttaaa aattattggg ttttttctcg tcctaggtgt tcttatagcg gcttgcgcac
     6001 ctacatccgc accggcaaca gaagttgcag aaactgaagc cgtttcacag gttcaaacag
     6061 aggcggcggc agaaaatgta ggtacgctta agattgctgt cctgcccatt atcgatacac
     6121 ttcccatata tgttgcagaa gccgaaggtc tatttgcaaa acacggtgtg accgttgagc
     6181 taatccccgt tgcgtcggcg cctgaacgag accagcttct tgcggcgggt caggcagatg
     6241 gcactatcaa tgaaacgtta gcggtaatgc ttttcaacaa agatgccgtg caaatgcagg
     6301 tagtacgtta tgccttgcgc cctggtgaag ggtatccaca ttttcgtatc cttgcttcaa
     6361 accagagtgg cattacacta tccaccgagc tgaagggagt agagattggc gtctcacaag
     6421 gaacaatcat tgaatatgtc accgagcgtt tgctaaaaac acaaggtctt gctcctgatg
     6481 aaattaagac catcgctgtg ccaaagattc cagaccgtat ggcattgctt ggttcaggtg
     6541 agctttctgc cgcagtgttg cctgacccac ttgcctcact tgccatgagt cagggtgcgg
     6601 tcaatgttct ggatgactcc atgaaccctg aatatgggtt tagtgtctat tcgttccgca
     6661 aagatgttat cgacgcgaac cctgaagccg taaaagcatt ccttgcagca atcgaagaag
     6721 caaggtctct gatcaaccca aaccccagca aatacacttc actattaagc gataagggac
     6781 tcatcccacc gccgctgttg gaaacattcc gcgtgccgcc ctttccacaa agtgaagtgc
     6841 caactgaagc cgaatggaac gatggtttgg catggctcaa agaaaaaggg attttgagcg
     6901 ttgatgtaag ttacgcagat tcggtcaata gttcgctgtt gccctaacaa atcaattggc
     6961 agccaccttt agtggctgcc aatttccatc tcatgatatc cattcaatca ctcaccttcg
     7021 aatataacca tcactcaccg ctcttccaga atttcaactg gacagtttca tgcggcgaga
     7081 catggtctat tctgggtcca tctggatgtg ggaaaacgac tttgttgtat ctgttggcag
     7141 gtttgaaatt cccaacctct ggcgaggtga gtatccatgg agagaaaatt caacgaccgc
     7201 gtccacatag cgggttgata ctgcaagatt acggcttgct gccttggtct accgtgcgcg
     7261 aaaatgtagc actcggcata aaagtccgca ccttttatgg ggcagatggc aaacacacgc
     7321 ctaataattt caccccgaaa gataatattg aaagttggat agaacgcctt ggattaagcg
     7381 aagttgcgga taaatttccc agtcaacttt ctggtgggca acgtcaacgc accgccattg
     7441 cgcggacgct cgcgctcgaa ccagacctgc ttctaatgga tgagcctttt tcttcgctgg
     7501 atgctgttac ccgcgaagac cttcaaaatt taacgctctc attatgtgca gagcagaata
     7561 taaccttggt aattgtgact cattctattg aagaagcggc agtgcttgga aaaaagatat
     7621 tattgctaag caacccgccc aaccaaactg tgcaagtatt tgataacccc aatgctggaa
     7681 caacaggttt ccgtcatacg ccagaatttc acagcatgtg cgaacacctt tggcgtgaaa
     7741 tgcaaaaagg aaatgtctct ctatgaaacg gcgtgacgtt ctttttgccg cactagcgat
     7801 tcttttggta tggcagctcg cagctatgat cgtgaaaatg cctattttgc ctagtccgtt
     7861 aaccgtctgt atggtcttct tcaaagagat gcaaaacggg ttgtatatcc attttgcggt
     7921 gagtttatgg cgcgtaacag caggaatgct gctctcggtc ttggtggcgg ctccagcagg
     7981 gttgttaatt ggcgggtcga agagtttgaa ccgtttgctc tcccccatta tttatttgct
     8041 gtacccaatt cctaaagtgg tctttgtacc ggttattatt ttgttcttag ggatcggcga
     8101 tacgtccaag atcgtcatta tgttcttgat tctgttcttt cagatcattg tacttgtccg
     8161 cgatcaggca agtggtattg cgccgcaatt gcttcaaagc ctgcaaagtc tcggtgcggg
     8221 caggcgcgcg ctcttccgtt tcgtttattt acccgctagt ttgccagcca ttctaactgc
     8281 actacgccaa tccattggca cggcggtggc ggtgttgtac atcacagaac tttcagcaac
     8341 aaaatatggg ttgggatatt acatctatta caaaggtagc acgttactag attacccgtc
     8401 aatgtatgcg ggtgtgatcg ccatgagcct gcttggactt ggcatgtact tcacagtcga
     8461 ttggctggag cgaaaatggt gcccgtggaa gtttgtcagt tagaaaaaag taacccaacc
     8521 caaaagaaaa ggctgaacaa aaaggctgtt tgcccagtgc ctgctaaagc cacattgagt
     8581 ggttttcctt tttcagtaaa aactgttctc aatgtacgaa tggcaatcgg gagggataac
     8641 catgcaagca atgcgccaat aggaataata ttcatccata ccaaaactgg cacgatcaaa
     8701 taagcaatca acatcaagag cagatattct gccttggcgc cctcttctcc aaagcggaca
     8761 gctaatgtgc gtttgcctgc tttacggtcg ttttcgaggt cgcgcaaatt gttgacgacc
     8821 aaaatcgcag tcacgatcaa cccaaccgga actgccatcc accaagcggc agggctgacg
     8881 gagccaactt gaacaaaata tgtccctgct acagcagcga gaccgaaaaa gagaaagaca
     8941 aaaacatcgc ccaagccatg atagccaaga gggtaaggtc cgccggtgta ggcaacagcg
     9001 gagagaatgg cagcagcgcc gagccagatc acggtccagc cgcgaagcgt ggcgagatat
     9061 aagcccaata ctgctgcaaa agcaaaaatc accaccatgc ccgtcttcac ttgcgatgga
     9121 gtcagatatc cagcctgagt cacacgcacg ggaccaagcc gttcagccgt atcggcaccg
     9181 cgttcaaagt caaacacatc attagcaaca ttgctaccga cctgcaacaa caatgccacc
     9241 aacaaagccg ccaacgcggg caacaattga aatgaaccgt catgccatgc caaagcactg
     9301 cccattgcaa ctcctgccgc cgccgcaggc aaggtgcgcg gacggatggc gagccaccaa
     9361 atttgaaaca tgttcggttt gtttgtttca gtcataacgt tgttaattca aaaagagttt
     9421 caaaccctcg ttggcataat aggcaaggct cgggctgagt aaatttgtaa ttgcccaaat
     9481 actttgccag aacatatttg aaatcggcga atcgatgaac acaaagagat acaaaatcaa
     9541 aaagccaaac gggcg
//
LOCUS       DAOQNJ010000028         9527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3455_length_9527_cov_11.233742, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000028 DAOQNJ010000000
VERSION     DAOQNJ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3455_length_9527_cov_11.233742"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="PLG52_01640"
     CDS             <1..1230
                     /locus_tag="PLG52_01640"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HNB40169.1"
                     /translation="NDLNETSWLNEGSSELAVLLNGYETGGFDTLYISDPDLQLNDWP
                     NDQGATSPHYGAGFLFMTYFLDRFGEEATQALVKDPANGLDSVDDVLQQIGATDKLTG
                     QPITADDFFMDWALTNLLLDPSIADGRYVYNNYPAAHRASATETIYNCPQEPLLRDVH
                     QYGVDYIAIECAGDFTINFTGSTVTGLLPADPYSGDYAFWSNKGDESNMTLTHEFDLT
                     NASGPVDLSFRTWFDIEEDWDYVYVEVSEDGETWQILTTPSGRGTDPSGNSYGWGYTG
                     VSGDWIEEKVDLSDYAGKKVFVRFEYITDAAVNGEGFMVDDVKVDAAGYSSDFEADDG
                     GWVAEGFVRVQNVLPQTFKLALIKSGDDNVTMIPVNADQTAEIPVSLKSGETAYLVVT
                     GTTRFTRELATYQIEIR"
     gene            1356..4481
                     /locus_tag="PLG52_01645"
     CDS             1356..4481
                     /locus_tag="PLG52_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNB40170.1"
                     /translation="MMTQSGNPSGKRKTIAVLGAQLSRVWGSEFMAGVLDAAHAVDFN
                     VVSFVGGKPSALQSHDGHSYGLYDLINPEQIDGVLLSADIAHGLSRDALQKFVEFLAP
                     LPVASFAVPSKGVPAVIADNDSGMRAIVRHLIEDHNYQRIAFVRGPVGQVEADQRYHA
                     FLDELKAHNIRFDQHLELEGDYSTESGRAAVRTLLDERGMAVQAIVASNDRMAFGVLE
                     ALQQRGITVPDKVAVTGFDDVSESQSMGVPLTTVRQSFYDAGAKTFELLLKRINGEEV
                     DDINLLPSSIVVRWSCGCLPESIHRAVVLPREVAHTGRLENKRVKAINALLAAAGVPE
                     TTPDRDAYVENFGVAWDTFLYSLRETDKSDAFLRSIQGMVEILQKHGYDFYTWQNVIS
                     TFRKYALGGISSTTTTLRAENLFQQARMLAGELSQRAQAYNRLQYVKQEELLNNFSFS
                     MASAVSFDDIGDAIARHFPILGIERWYVMYYGETDDAPGSISSPPPQSYRLLMQYDDK
                     RFTMPDDRSRLATGRLVPRGKTPEKRRYDAVVMPLSLATNRFGFMWAEMGPDDWDIYV
                     RVRNLLSSALLRTMLVNQREQAKREVDQLYAAEQARRHGAETLARSMRQLSSLKNVEQ
                     LPEQILEQLSQALPYDRGVLFMEDVNGEPQIQAHRGMPTNADMASFRLGMNGNDFYTT
                     VSRKGETLVVSDINLVENWSQPEWLVRDRSWMGVPLYLKDNVIGLLMVSRADRPFTHD
                     DGLLATTFASQATVALENARLYHEVTGINQMMERMVSERVAELNETYKTLEKHDKNKS
                     AFIQVAAHELRTPLTVVKGFLGMLQNDATVRSNSMLSDAVEGVLRGTNRLHQIVNSML
                     DVVRLENQVITPHLEAVSLGLILRLIHKDYVDDLATRNLTFTLDDSVKTIPPMLADSE
                     LVKKALDQVIVNAIKFTPDGGSIYISAWVVEDPKRGKMAEIAIKDSGIGIDPEHHKVI
                     FEKLYQMGEVQLHSSSRTNFKGGGAGLGLAIASGIVRALQGTIWVESPGKDEEKYPGS
                     TFFIRLPLLKE"
     gene            complement(4518..5420)
                     /locus_tag="PLG52_01650"
     CDS             complement(4518..5420)
                     /locus_tag="PLG52_01650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB40171.1"
                     /translation="MTNTPPVCNYEGSDYQQSFWDQGGRAYEDQSEAIALRRMLPKSG
                     KLMLEVGAGAGRNTPRYAGFDRIVVMDYSTTQLAQAQERLGRSDKYIYVAADVYRLPF
                     RNGLFDGATMIRVLHHMADAPKALGQIQGTLQPGATFILEFANKLNLKAILRYWLGKQ
                     TWSPFTLEPVEFVKLNFDFHPKAIRQWLGNLGFNIEKTLTVSHFRLGLLKRTIPSKLL
                     AAVDGLFQPTGALFQLTPSVFVKAVLPGASSADIPLNVIDLFKCPECNSGGLQEKTDH
                     VFCPSCKAKWSVKDGIYDFREKIS"
     gene            complement(5510..6805)
                     /locus_tag="PLG52_01655"
     CDS             complement(5510..6805)
                     /locus_tag="PLG52_01655"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNB40172.1"
                     /translation="MKQICVVGVGYVGLVTAACFADLGNRVVALDVNEERVANLKKGI
                     MPIYEPGLDELVKRNVKAGRITFTSSYTEALKGAEYAFIAVGTPSGSDGSADLQYVAA
                     AATSIAKNMTAPLILINKSTVPIGTGDWVADIVKGAQPKPIDFAVVSCPEFLREGSAI
                     GDFMNPHRTVIGSLDRDAANKVAQLHLPLRAPIVITDLRTAEMIKYASNAFLATKISF
                     INELADLCERVGADVKEVAAGMGYDARIGRHFLDAGLGWGGSCFPKDVEALAFMAKEK
                     GLEPKILNSVMDVNYDRRKEAVKVVENMVGGSLKGKTIGLLGLAFKPNTDDMRDAPSI
                     DISAELIEAGAKVRGYDPVAMSVARPILPAVEMYDEPYKLAEGCDALMIITEWNEFKQ
                     LDLDKVKSLLKSPVIYDGRNIYEPGRMKDMGFTYRAIGR"
     gene            complement(6890..7264)
                     /locus_tag="PLG52_01660"
     CDS             complement(6890..7264)
                     /locus_tag="PLG52_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB40173.1"
                     /translation="MSAGRILIVEDNMDTYELVRFILEKNGFETFLAMNGRDGVNAAT
                     KQRPDLIIMDLSMPEMDGWTAMRLIKSDPAISSIPMIALTAHALPSDRKRAFDAGCDE
                     YITKPMDLIDLLDTVKSWIQKV"
     gene            7329..8495
                     /locus_tag="PLG52_01665"
     CDS             7329..8495
                     /locus_tag="PLG52_01665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB40174.1"
                     /translation="MRLLFVTDARSPISKNWIRHFIERGDEVFIASTFEWDVEGIPSE
                     RTEFTPVAFSSAKKRTSAPSTASSRTLSLRTKIRQWLGPVTIPAAAFKLRAFIREVQP
                     DIVHAMRVPYEGMLTAEALQGLKAHPTFLVSIWGNDFTLHAPSTPLMRYYTRKVMNSV
                     DGLHADVERDVKLAHEWGLSAEKPTLVTPGNGGVRSDVFYPPKELVKSPIIINPRGVR
                     PYVRNDSFFKAIPLVLAKYPDAKFLCVGMQNEPQAMGWVQEYEIENSVELLPAFSHEK
                     MGEVFRGAQIVVSPSIHDGTPNTLIEAMACGCFPIAGDLESIREWISHGQNGLLFDSN
                     NPQSIADAMLLGLEREDLRTEAAGLNKKIISARAEFSTNMNKVEKFYSVLRGTN"
     gene            complement(8492..8713)
                     /locus_tag="PLG52_01670"
     CDS             complement(8492..8713)
                     /locus_tag="PLG52_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNB40175.1"
                     /translation="MRRMFGFMIGIFTGALVGSVIALLLAPESGENLRGQLRNRGQNF
                     LNDVRHSADARRIELRNHLESLRSSREEF"
     gene            complement(8842..>9527)
                     /locus_tag="PLG52_01675"
     CDS             complement(8842..>9527)
                     /locus_tag="PLG52_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB40176.1"
                     /translation="ENEIAQSESYTGKEFSRYWIHNGMLQLGGEKMSKSLGNIISIKD
                     FLSKRSSDVMRMLVLNGTYRAPLMFNDETLDAAERNVERLKSALKPASPSASGLTSDA
                     LSASVESAATNFTDAMDNDFNTAGAVAALFELSKSINTARDNGATADQLKPAQETFKQ
                     LAGVLGLKLEEKKGSSEKEAEVEALLAERTQARKDKNWKRSDEIRDQLKAMGVTIEDS
                     KDGTTWKWS"
BASE COUNT         2300 a         2229 c         2468 g         2530 t
ORIGIN      
        1 aatgacttga acgaaacctc ctggttgaac gaaggttctt ctgaattggc ggtcttgctc
       61 aacgggtatg aaactggtgg ttttgatacg ctttatatct cagacccaga cttgcaatta
      121 aatgactggc ccaatgatca gggtgctacg tcgccgcatt atggggcggg cttccttttc
      181 atgacatact tcctcgaccg tttcggtgaa gaagccaccc aagcactcgt gaaagatcca
      241 gccaatgggt tggatagtgt ggacgatgtc ttacaacaaa tcggtgcgac cgataaattg
      301 accggtcagc cgattacagc agatgatttc ttcatggatt gggcactgac gaatcttttg
      361 ctcgacccct ccattgctga tggacgctat gtgtacaaca attatccggc ggcacatcgt
      421 gcttctgcaa ctgaaacgat ttataactgc ccgcaagagc cgcttctgcg cgatgttcac
      481 caatacggtg tggactatat cgccatcgaa tgtgctggcg actttaccat caacttcacc
      541 ggctctaccg tgaccgggct tttgccagct gacccctatt ctggtgacta cgccttttgg
      601 tcgaacaagg gcgatgagtc caacatgaca ttgacgcatg aattcgatct caccaatgcc
      661 agcggaccgg ttgatttgtc cttccgcact tggtttgata tcgaagagga ttgggactat
      721 gtgtatgtgg aagtctctga agacggcgag acttggcaaa ttctcactac accttctggt
      781 agaggcacag acccgagcgg caattcgtat ggctgggggt ataccggtgt ttctggcgat
      841 tggatcgaag agaaggttga cctttctgat tacgctggta agaaagtctt tgtccgcttt
      901 gagtacatta ccgatgctgc ggtcaatgga gaaggcttca tggtagatga tgtcaaagtc
      961 gatgcagcag gctactcgtc tgattttgag gcggatgatg gcggttgggt ggcggaggga
     1021 ttcgtgcgcg tccagaatgt cctcccgcaa actttcaagc tggcgctcat caaatctggc
     1081 gacgacaatg tgactatgat cccggtcaac gcagaccaga cagcagagat cccggtttcg
     1141 ttgaaatctg gtgaaacggc ttacttggtt gtgaccggaa cgacccgctt cacacgtgag
     1201 ctggcaacct atcagatcga gatccgctaa gcttttctga aatttctaca ttgacagccg
     1261 cttttatggc ggctgtcaat ttttttaagg cggagattgt cttgcaaaat tgcaattttt
     1321 ggctcaggca tcctgccgta taattttgta actccatgat gacccaaagc ggaaatcctt
     1381 ctggcaagcg aaagacgatc gccgtcctcg gtgcacaact cagccgagtg tggggcagtg
     1441 agtttatggc gggcgtgttg gacgccgctc atgcagtgga tttcaatgtc gtttcttttg
     1501 taggcgggaa accttcggcg cttcaatctc atgatggaca ttcttacggt ttatacgacc
     1561 tcattaatcc cgaacagata gacggtgttt tgctctctgc agatattgcc cacgggcttt
     1621 cacgagatgc acttcaaaag tttgttgaat ttctcgcgcc tttgccggtt gcttcattcg
     1681 ctgtgccaag caagggcgtt cctgctgtga tcgcggataa tgacagcggt atgcgtgcaa
     1741 tcgtacggca cttgatcgaa gaccataatt atcaacgcat tgcctttgtc cgcggaccgg
     1801 ttggtcaggt ggaagcagac cagcgctatc acgcattcct tgatgagttg aaagctcata
     1861 acattcgttt cgaccaacat ttggaattag aaggcgatta ttccaccgaa agtggccgtg
     1921 ccgcagtgcg caccttgctt gatgaacgtg gcatggcagt gcaagccatt gtcgcttcga
     1981 atgaccgaat ggcattcggc gtgttggaag cgttacaaca gcgcggtatt acagtgcctg
     2041 ataaggttgc ggttacgggt ttcgatgatg tcagcgagtc gcaatctatg ggggtgcctt
     2101 taaccaccgt ccgccaatcg ttttatgatg cgggtgcaaa gacatttgaa cttctcctga
     2161 aacgcatcaa tggcgaagaa gtggatgata ttaatttgtt gccatccagc attgtagtgc
     2221 gttggtcttg tggttgttta ccagagagta ttcatcgtgc agttgtgttg ccacgtgaag
     2281 tagcgcacac aggccggctt gaaaacaaac gtgtgaaagc catcaatgct ttgcttgcag
     2341 cagcaggcgt gcctgaaact acacctgatc gtgatgccta tgttgaaaat tttggtgtgg
     2401 catgggacac cttcctttac agtttgcgcg aaaccgataa gagtgatgcg ttcttacggt
     2461 ctattcaagg catggttgaa atccttcaga agcatggtta tgacttctat acctggcaaa
     2521 acgtgatctc tactttccgt aaatatgcgt taggtggcat ttcaagcaca accactacat
     2581 tgcgtgctga aaatcttttc cagcaggcac gtatgttggc gggcgagttg tcgcagcggg
     2641 ctcaggcata taaccggctt caatatgtaa agcaggaaga actgcttaat aactttagtt
     2701 tttccatggc atcggctgtt tcgttcgatg atatcggcga tgcgattgct agacatttcc
     2761 ccattcttgg tattgagcgc tggtatgtga tgtattatgg cgagacggac gatgcgccgg
     2821 gttcgatttc ttcaccgccg ccgcaaagtt atcgtttgct catgcaatat gacgataaac
     2881 gcttcaccat gccggatgat cggtctcgac ttgcaacagg tcgccttgtg ccacgcggaa
     2941 agactcctga aaaacgtcgt tacgatgccg tagtcatgcc gctctctttg gcaacaaatc
     3001 gcttcgggtt tatgtgggca gaaatgggac ccgatgattg ggatatttac gtccgcgtgc
     3061 gaaacttgct ttcaagcgcg ctcttgcgta cgatgttggt taatcaacgt gagcaagcca
     3121 aacgtgaagt ggatcagctt tatgcggcgg aacaggcacg gcgtcatggt gctgagacgt
     3181 tggctcgttc gatgcgccag ttgtcttcac taaaaaatgt tgaacagtta ccagaacaga
     3241 ttctagaaca attaagccaa gcattgccgt atgatcgtgg cgtgctgttt atggaagatg
     3301 taaatggtga gccacagatt caagcccatc gcggtatgcc aaccaatgcg gatatggcat
     3361 ctttccgctt gggaatgaat ggtaatgatt tttacacgac cgtttcacgc aaaggcgaga
     3421 ctcttgtggt gagcgatatc aacttggttg aaaattggtc tcaacccgaa tggcttgtcc
     3481 gtgatcgttc ctggatgggc gtgcctttat acctcaaaga taatgtcatc gggctcttaa
     3541 tggtcagccg tgctgataga ccatttactc acgatgatgg tttgctagct acaacatttg
     3601 cttctcaagc aaccgttgct ttggaaaacg cccgtttgta tcacgaagtg actggcatta
     3661 atcaaatgat ggaacgcatg gtttccgagc gtgttgcgga actgaatgaa acctataaaa
     3721 cacttgagaa gcacgataag aacaaatctg cgttcattca ggtggcagca catgaactgc
     3781 gcacacctct gacggtggtg aagggttttc ttgggatgtt gcaaaacgat gccaccgtcc
     3841 gttcgaattc aatgctttcc gatgccgtag aaggggtctt gcgcggcacg aaccgtttgc
     3901 atcagatcgt aaacagcatg ttggatgtgg tgcgccttga gaatcaggtc atcacgccac
     3961 atttagaagc ggtcagtttg gggctgatct tacgcctcat tcacaaagat tatgtggatg
     4021 atctcgcgac tcgtaacttg acctttacat tggacgactc tgtaaaaacg atccctccaa
     4081 tgctagcgga ctctgaactc gtgaagaagg cgcttgatca agttatcgtc aatgccatca
     4141 agttcacacc tgatggcggc tcgatttata tctcagcttg ggtagtggaa gatcccaaac
     4201 gcggcaagat ggcagagatc gccatcaaag attctgggat tggtattgac cccgaacacc
     4261 ataaagttat ttttgaaaag ttgtatcaaa tgggcgaggt gcaattacat tcgtctagcc
     4321 gaaccaattt caagggtggt ggcgctgggt tgggacttgc cattgcctct ggtattgtcc
     4381 gtgccttgca gggaacgatt tgggtggaaa gccccggcaa agatgaagag aaataccccg
     4441 gcagcacctt cttcatccgc ttgccgttgt tgaaggaata acaaaaaacg cgctcaatgt
     4501 agagcgcgtt tttttttcta acttatcttt tctctaaaat cataaatccc atccttgaca
     4561 gaccactttg ccttgcaaga aggacaaaat acatggtctg ttttctcttg caaccctcca
     4621 ctgttacatt cggggcattt gaagaggtca atgacattta acggaatatc tgcactggag
     4681 gcaccgggca aaactgcttt tacgaatacg cttggcgtaa gttggaagag cgcacctgtt
     4741 ggctgaaaga gaccatccac agcggcgagt agtttagaag gaatagttcg tttgagcaaa
     4801 ccaagccgaa aatgtgagac ggttaaggtc ttttcgatat tgaaaccaag atttcccaac
     4861 cactgacgaa tagccttggg gtgaaagtca aagttcaatt tgacgaattc caccggctcc
     4921 agcgtgaacg gactccacgt ttgcttgccg agccaatagc gcagaatcgc cttgaggttg
     4981 agcttgttcg caaactcaag gataaaagtt gccccgggtt gtagtgttcc ctgaatctgc
     5041 cccaacgctt tgggcgcatc tgccatgtga tggagcacgc ggatcatggt tgcgccatcg
     5101 aatagtccat tacggaaggg gaggcggtag acatctgcag cgacgtaaat gtatttgtcg
     5161 gagcgaccca gcctctcttg cgcctgagcg agctgagtcg tggagtaatc catcaccaca
     5221 atccggtcaa agcccgcata gcgaggggtg ttgcgtccgg caccggcgcc tacctccagc
     5281 atcagcttgc cgcttttggg caacatgcgt cttaatgcaa tggcttcaga ttggtcttcg
     5341 taagcgcgcc cgccttgatc ccaaaaggat tgctgatagt cggagccttc atagttacaa
     5401 acaggaggag tattggtcat aattctgttc tttttgtgtt taaaaaattg gtagtcgagc
     5461 cgtcagattg tacccgacta ctcgactacc aaacaaatga actgaaaaat tagcgtccga
     5521 ttgcgcggta ggtaaagccc atgtctttca tacgaccggg ttcgtagata ttacgtccat
     5581 cgtaaatgac cggggatttg agcaagcttt tcactttgtc gaggtcgagc tgtttgaact
     5641 cattccattc ggtgatgatc atcaacgcat cgcagccttc ggcaagtttg taaggttcat
     5701 cgtacatttc cacagccggg agaatcgggc gtgccacact catggcaacc gggtcatatc
     5761 cgcggacctt ggcgcctgct tcaatcaact ctgccgagat atcaatggag ggcgcatcac
     5821 gcatgtcgtc ggtattgggt ttgaaagcca agccaagcaa accaatggtc ttgcctttga
     5881 ggctgccgcc gaccatgttt tccacaacct tcacagcttc cttgcggcgg tcgtagttaa
     5941 catccatcac gctgttcaga atcttcggtt ccagtccctt ttcttttgcc atgaaggcga
     6001 gggcttctac atctttgggg aagcaagagc cgccccaacc aagacctgca tcaaggaagt
     6061 gacgtccgat gcgggcatcg tagcccatac ctgctgcaac ttcttttaca tctgcaccaa
     6121 cgcgctcgca caagtcagcg agttcgttga tgaaggaaat tttggtggcg agaaaagcat
     6181 tgctggcgta cttgatcatc tctgccgtgc gcaagtcggt gatgacaata ggggcgcgca
     6241 aaggtaagtg caattgagca accttgtttg cagcatcacg gtccaaggaa ccgatcacgg
     6301 tgcggtgcgg attcatgaag tcgccaatag cggagccttc acgtaaaaac tcggggcaag
     6361 agacaacggc gaagtcgatc ggtttgggct gggcaccctt cacaatatcg gcaacccagt
     6421 caccggtccc gatcggaacc gtggacttgt tgatcaggat caatggtgcg gtcatgttct
     6481 tcgcaatgga agtggcagcg gctgccacat attgcaaatc agcagaccca tcagacccag
     6541 aaggcgtacc cacagcaatg aaggcgtatt ctgcaccttt cagcgcttct gtataggagc
     6601 ttgtgaacgt aatacgccct gcttttacat tgcgtttgac aagttcatcc aagccgggtt
     6661 cgtaaatggg cataatgccc tttttgagat tggcaacgcg ttcttcattg acgtccaagg
     6721 caaccacgcg gttacctaaa tcggcaaaac aggcagcggt cacgagaccg acgtagccca
     6781 cgccgacgac acaaatttgt ttcatggtgt atttcctttt ttgaagattt ggctctttaa
     6841 tatgagactt tgtatattgc tataagccat ttattttccc tagcctgatt tatacttttt
     6901 gaatccaact cttaacggta tccaacaaat cgatcaggtc catgggtttg gtgatgtatt
     6961 catcgcagcc tgcatcgaac gcacgtttgc ggtcactggg cagggcatga gcagttaggg
     7021 cgatcatagg aatggaagat atggcagggt cgcttttgat caaccgcatg gcggtccaac
     7081 catccatttc aggcatggaa aggtccataa tgatcaagtc tgggcgttgt ttagtggcag
     7141 cattgacgcc gtctcgtcca ttcatagcga ggaaggtttc aaaaccgttc ttctccagaa
     7201 taaaacgtac cagctcgtag gtatccatat tatcttcaac aataagaatg cgtcctgcgc
     7261 tcatgcgctt aaatatacta caaagtaggt tgcgatagcc caatattttt acaatgtata
     7321 atcaccttat gcgcctgcta tttgtcacag atgcccgttc ccccatctca aaaaactgga
     7381 ttcgtcattt tatcgagcgc ggagacgagg tttttatcgc ctctaccttt gaatgggatg
     7441 tggagggtat cccctccgaa cggactgaat tcacccctgt ggcatttagc tctgctaaaa
     7501 aacgtacctc tgctccctcc accgcctctt cccggaccct gagcctgcgc accaaaattc
     7561 ggcagtggct tggtcctgtt accattcccg ctgccgcgtt caaattgcgg gctttcatta
     7621 gagaagtcca gccagatatt gttcatgcta tgcgcgttcc ttatgaaggc atgttaaccg
     7681 ccgaagccct acagggactc aaggctcatc ctacatttct cgtttccatt tggggcaatg
     7741 actttaccct gcatgcccca tctacaccgc tgatgaggta ttacactcgc aaggtgatga
     7801 attctgtaga tggtttgcat gcagatgtag agcgggatgt aaaactggca catgaatggg
     7861 gcttgagtgc agagaaacct acgttggtga cacccggtaa cggcggagtt agaagcgatg
     7921 ttttttatcc gccaaaagag ttggtgaaaa gcccaatcat catcaaccct cgtggagtaa
     7981 gaccctatgt gcggaatgat tcgtttttta aggcgatacc cttggtgctg gcaaaatatc
     8041 ctgatgcgaa gttcttatgt gtaggtatgc aaaatgagcc gcaagccatg gggtgggtgc
     8101 aagagtatga aattgaaaat tctgtggagt tgttgcctgc cttctcgcat gaaaagatgg
     8161 gagaggtgtt tcgcggtgca cagatcgtgg tttcccccag tattcatgat gggacgccaa
     8221 acacgctgat cgaagccatg gcgtgtggat gtttccccat agcaggtgac cttgaatcca
     8281 ttcgtgagtg gatttcacat gggcaaaacg gtttgttgtt tgactcaaac aacccgcaat
     8341 cgatagctga tgcaatgtta ttaggtctag aaagagaaga cctgcgcacc gaagccgcag
     8401 gtctgaataa gaaaattatc tctgcacgtg cagagtttag tacaaatatg aataaagtgg
     8461 agaagttcta ttctgtatta agaggtacga actagaattc ttctcttgag gagcgaagag
     8521 actctaagtg attgcgtaat tcaatgcggc gggcatctgc cgagtgacgc acatcgttga
     8581 ggaagttttg cccgcggtta cggagttgtc cgcgcaggtt ttcaccggat tcaggggcga
     8641 gaagaagtgc aatgacagag ccgactaacg cgccggtgaa gatgccgatc ataaagccaa
     8701 acattctacg catggaattt ctccttgaaa attcgttttg ataattgaat tatacatctg
     8761 aaagttgtta caaataaaaa cttccgagaa acatttctcg gaagttttac tattcaactg
     8821 attgactgtc acactacgaa actagctcca cttccaggtg gtgccgtctt tgctgtcttc
     8881 gatggtgacg cccatcgctt tgagctggtc gcggatctcg tcggagcgct tccagttttt
     8941 gtctttgcgg gcttgggtgc gttcggcgag gagggcttcc acttcggctt ctttttcgct
     9001 ggagcctttt ttctcttcaa gtttcaagcc aagcacgcca gccaattgct tgaaggtctc
     9061 ttgcgctggt ttgagctgat ctgctgtcgc gccgttatcg cgggcggtgt tgatggattt
     9121 ggaaagctcg aagagtgcag caacagctcc ggcagtgttg aagtcattgt ccatggcatc
     9181 ggtgaagttg gttgctgcgg attcgacgga agcggacaag gcgtcagagg tgagtccgct
     9241 ggcggagggg gaggcaggct tgagcgcaga tttgaggcgt tcgacgttgc gttcggcggc
     9301 atccagcgtt tcgtcattga acatcagcgg ggcgcgatag gttccgttca agaccaacat
     9361 acgcatcacg tcagaggaac gtttggagag gaagtctttg atgctgatga tgttgcccaa
     9421 agacttggac attttctcgc cgccgagttg cagcatgccg ttgtggatcc agtagcgcga
     9481 gaattctttg ccggtgtagc tttcgctttg ggcaatttcg ttttcgt
//
LOCUS       DAOQNJ010000029         9478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3498_length_9478_cov_9.348297, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000029 DAOQNJ010000000
VERSION     DAOQNJ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3498_length_9478_cov_9.348297"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PLG52_01680"
     CDS             complement(<1..541)
                     /locus_tag="PLG52_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNB40177.1"
                     /translation="MNTPLSGAPQRLTGQDLIRLKKYLADNLLRALETEGIEASQRVT
                     YIQQNIGRVFDTTQLKFPEDLKREIFKQVLNDLLGFGPIQPLLDDPDISEIMVNGPKK
                     IFIEKKGQLTKSEITFDDDDHVLRVIDRIIMPLGRRVDFDSPTVDARLPDGSRVNAVV
                     RPVSIDGPSITIRKFRKDKL"
     gene            complement(538..831)
                     /locus_tag="PLG52_01685"
     CDS             complement(538..831)
                     /locus_tag="PLG52_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40178.1"
                     /translation="MSIEYDDKGKYYTNVIQKVPVPAMVQTTTNLIRGIVHVRHGERL
                     KDEIEGSEQFVAMTDVSVYSADGKELYTGPFLALQKGQIVWVMPLNETQKGEE"
     gene            complement(864..2012)
                     /locus_tag="PLG52_01690"
     CDS             complement(864..2012)
                     /locus_tag="PLG52_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB40179.1"
                     /translation="MSQKKILIVDSDVASRNFIARKLQELDYETLQAGSGKEGLIYTW
                     RDHPDLVIADPALTDIKGEEFATKLRNDPRTANLPIVALSSDHNPARIKSCQDAGFND
                     YITKSGQAVPMLKDIINRLFGVTQEDMKQGGLMIVFLSAKGGTGTSSVCANTAVNITK
                     DQPEARIAVVDLVFPIGSIAQIVGYEGTDNIVTVADRNPLETDVDFLKTLPRIEPWNF
                     NLLAGSPDPELSNQLNVGRIWDIITGLKSSYDYVLIDIGRSLSKITLPLIQHADLISL
                     LISTDVSTISLTKTILDFLKSKGVKPEAIYTILNRHASLEGLSKREAETILEKNVNTA
                     IPYLGTNFGFANSQHRPFTLKFPNDTASIIFHDSAREMANLAKKIRSQ"
     gene            complement(2051..2746)
                     /locus_tag="PLG52_01695"
     CDS             complement(2051..2746)
                     /locus_tag="PLG52_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB40180.1"
                     /translation="MAKRILIVDDEPRYLRLLEANLRTEGYEVVTAQDGLQALDVFSS
                     NPIDLVLMDVMMPRLDGFGATQRLREFTNVPIVILTARGEEQDRVRGLDLGADDYLIK
                     PFSATELLARVRAVLRRAHNPTDAGQVRFFTHDDLKIDFARAEVWRGEEAVSLSATEY
                     RLLLQFAHNVGKILTSEELLTSVWGVEYKNDKEILWVSIARLRQKLEKDAHTPKHIVT
                     RSGLGYLMPPIET"
     gene            complement(2782..3969)
                     /locus_tag="PLG52_01700"
     CDS             complement(2782..3969)
                     /locus_tag="PLG52_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNB40181.1"
                     /translation="MDKETLSDLHRIFQDVQAKPDWRSALDALFISMRRSFVFDNVAV
                     YLSERQTRGLDVVYARAIGRGKTAEADAAWGESVANDVITNEHMVIHGPAKGSNHRDR
                     MSQTFVLGIPLYVGEKLRGALLFVRFGGPEYSDIHIHVASLQAIWAAALIERKELQEA
                     REELDSVQRQMRLQDDFVSTISHELRTPLGFIKGYSTSLLRQDTQWDDATQREFLSII
                     DEETDRLAKLIEDMLESARLQSKTIQFKFSPIRLDAVVRDVAARVKTHHPNLVIEMNM
                     DVLPPVLGDGVRLSQVFENLFSNAIKYAPGSKLIVGGSHQKKKVRISFADEGDGIPEE
                     FLPFLFERFYRVPGERTVTGTGLGLYICKQIVMAHHGNIWVESVLDRGTTFFIELPAD
                     PTL"
     gene            complement(3973..4995)
                     /locus_tag="PLG52_01705"
     CDS             complement(3973..4995)
                     /locus_tag="PLG52_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB40182.1"
                     /translation="MESKSPKLSPEMLVPRLGEYLVQKGLISEIDLQRALNHQQDVTY
                     KGGQSLLGQALIELKLIDKDTLDQVITEQIIQLRSALQSANRNLEQRVRERTSDLNEA
                     LNRLSELSQMKANFVSNISHELRTPLTHIKGYIELMLAESLGPISDEQRHALTVSQRS
                     TGKLEDMIEDLIMFSLASRGEMSMKLEGVDIRRLVSLVTKTAMRKAEDRGVKLEVLVK
                     DDTPYVQADGEKISWVVGQLIDNGIKFTPSGGSVTVAIEEEGKNLVQVIVRDTGVGIP
                     QDRMKEIFEPFHQLDGSSTRRYGGTGLGLSLVRQIVEGHGSLLDVKSTEGKGTTFKFP
                     LLAMRD"
     gene            complement(5037..6614)
                     /locus_tag="PLG52_01710"
     CDS             complement(5037..6614)
                     /locus_tag="PLG52_01710"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNB40183.1"
                     /translation="MKLLYNANIHTLDSANSHASAILIAGGRIIAVGSKDQLESLAHG
                     KVEKQDMKGKTILPGLTDAHIHMQFYSLGLSKVDCETKTKEECLRRVGERARTLKPGE
                     WVLGHGWQQNDWDGNFPTAAELDAVSPNNPVYLTAKSLHAAWANTSALKLANITDETP
                     DPKDGAVQRDANGKATGVMIETAMALVNAAVPEPTLAELEAAFEKAQSVLWKMGITGV
                     HDFDRRQSFMALQSLRAKGKLKLRVCKNIPVESVEQANDLGLRTGFGDEMLWIGSVKA
                     FMDGALGPRTAAMFQPYEGEKENRGILNMDGEELFEHARKAADVGLSMTVHAIGDKAN
                     HEVLNAFEQLRNYETQNHLPHLRHRIEHVQVIHPDDAPRLAKLNVIASMQPVHATSDM
                     FAADRYWGKRAELSYAWRTQLNYGAILAFGSDAPVESPNPFWGLHAAVTRQRADGSPS
                     AEGWRAEQKLTLAEALSAYTFGAAYTANAENRLGKLAENYHADLIVLDEDIFKIDPST
                     LLTMSPSAVMIDGEWVA"
     gene            complement(6688..7560)
                     /locus_tag="PLG52_01715"
     CDS             complement(6688..7560)
                     /locus_tag="PLG52_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNB40184.1"
                     /translation="MAQRKGLGKGLDALIPGGKSSTPAVTSSKTSGEGGVQQVPVEAI
                     KRNPRQPRVQFKEEDLNDLAASIREHGVIQPLIVSPKGDGTFILIAGERRWQASQRAG
                     LTKVPVVTRQANNQELLEIALIENVQRADLNAMEEAEAYHQLAEDFGLSHEMIAKRVG
                     KSRVAVTNTMRLTNLADAVKQALVDNTITEGHARALLGLSTQKAQTSALQTIINLSLS
                     VRQSEELIRKLTGVKPVKVKKATVSADVLDVEKRLQRSLGTKVALKHGKKGGTVTIYY
                     YSDEELDALIKKMS"
     gene            complement(7627..8397)
                     /locus_tag="PLG52_01720"
     CDS             complement(7627..8397)
                     /locus_tag="PLG52_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNB40185.1"
                     /translation="MTKIYTLVNQKGGVGKTTSTINLAAYLAKLGQRVLVVDLDPQAN
                     ATSCLGVDKLLVEGSTYDALLGEDDIFPFILLNERLQLSLLPSSPSLAGAEVELVDEL
                     ARESRLRKALLKVASRYDYVLIDCPPSLGLLTVNGLMAAIDGVVVPVQCEYLALEGLG
                     QLTETIERVRAALFPDLKVRGVILTMFDNRTNLSADVVREVNKHFPNQVFKSIIPRNI
                     RLAEAPSYGLPISEYAPNSPGADAYETLAKELLKGDKN"
     gene            complement(8394..9236)
                     /locus_tag="PLG52_01725"
     CDS             complement(8394..9236)
                     /locus_tag="PLG52_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNB40186.1"
                     /translation="MSDLRPVDYLLIGHVAVDLTPSGKQLGGTVSYAALTAKAMGLRV
                     GIVTSAGEDAPLSLLDGVQIFNLPTEQSTIFENVKTEHGRKQTLHHQATKISFEHVPQ
                     IWRDAAIVHLAPIAQEVDPALVGQFPASWVGVTPQGWMRGWDEKGSVFATAWENSEQV
                     LGQVGGVVMSLEDINHDLELGEWLAHHARLLCLTEGEEGAVLHWHGDRRRYRPIKVEE
                     VDATGAGDIFAAGFFSRLHQTKDPWEAARFATHLAAYSVTRVGLQGIPTHDEIEHCKM
                     EVLS"
BASE COUNT         2384 a         2522 c         2116 g         2456 t
ORIGIN      
        1 tcagtttatc tttgcgaaac ttacggattg tgattgaagg tccatcaatg gagacaggtc
       61 gaactacagc attgacgcgg gagccatctg gtaaacgagc atcgacggta ggtgagtcaa
      121 aatccacacg gcgacccaat ggcataatga ttcggtcaat aacacgcaat acatgatcgt
      181 catcatcaaa ggtgatctcg ctcttggtaa gctgcccctt tttctcgatg aaaatctttt
      241 taggcccatt caccatgatc tcagaaatat ctgggtcatc caaaagtggt tggatgggac
      301 cgaagcccaa caggtcattc agaacctgct taaaaatttc tcgttttagg tcttcaggga
      361 atttgagctg tgtagtatca aatacacgcc cgatgttttg ctgaatatat gtcactcgct
      421 gcgatgcttc gatcccttct gtttcgagtg cacgtaaaag attatctgcc agatacttct
      481 tcaggcgaat aaggtcctgc ccagttaacc gttgtggagc gcctgaaagt ggtgtgttca
      541 ttcttcccct ttttgtgtct cattcaacgg catgacccaa acgatctgcc ctttttgtag
      601 agccaagaat ggacctgtgt acagctcttt cccatccgcg ctataaacac tgacatccgt
      661 catggcaaca aattgctcgc tgccttctat ctcatccttc aaacgttcac catgccgtac
      721 atgaactatt ccacggatga gatttgttgt ggtctgtacc atggctggca caggcacttt
      781 ttgaataaca tttgtgtagt actttccttt atcgtcatat tctatagaca tgggctttat
      841 ctccctctgc ccgggaaaga cacctattgg ctacggatct tttttgcgag gtttgccatt
      901 tccctggcag aatcatgaaa aattatagag gctgtatcgt tagggaattt taatgtaaaa
      961 gggcggtgtt ggctgtttgc aaatccgaaa tttgtaccga ggtagggaat tgcagtattt
     1021 acatttttct ccaaaatcgt ttcagcttct cgtttgctta gaccttccaa actagcatgg
     1081 cgatttaaga tggtatagat tgcttctggc tttacccctt tcgatttgag aaaatccaat
     1141 atggttttgg tcaacgagat ggtgctgaca tctgtgctaa ttaacaaaga aatcaagtca
     1201 gcatgttgaa ttaaaggcag tgtaatcttt gagagagagc gcccaatgtc aattaggaca
     1261 taatcatagg aagatttcag cccagtaata atatcccaaa tgcgccccac attcagttgg
     1321 ttactcagtt cagggtctgg agaacctgcc aagaggttga aattccaagg ctcgattctg
     1381 ggcaaagtct taagaaaatc cacatcagtt tccaatgggt ttcggtctgc aacagtgaca
     1441 atattatcag tcccttcgta gcctacaatc tgagcaatgg acccgatagg aaataccaaa
     1501 tccaccacgg caattcgtgc ttcgggttga tccttcgtaa tattgaccgc tgtattggca
     1561 caaactgaag aagtacctgt gccacctttg gcactgagaa aaacgatcat caaaccaccc
     1621 tgtttcatat cttcttgtgt aacgccaaac aagcgattga tgatgtcttt caacatcggt
     1681 accgcttgtc ccgatttcgt aatgtaatcg ttgaagcccg catcctggca agatttgata
     1741 cgagcagggt tatggtcact acttaaagcg acaataggca aattagcagt ccgcgggtcg
     1801 ttgcgtagtt tagtagcaaa ctcttccccc ttgatatcgg tcaaagcagg gtcagcaatc
     1861 acaaggtctg gatggtcgcg ccatgtataa atcagacctt ccttaccaga ccctgcctgt
     1921 aaggtttcat aatcaagttc ttgtaatttt cgtgcaataa agtttcggct tgcgacatct
     1981 gagtctacga tcaaaatttt cttttgagac atatcttgtt cctgagcgca acccgtttta
     2041 tgcgcaagta ttacgtttca atagggggca tcagataccc tagcccagaa cgggtaacaa
     2101 tatgttttgg ggtgtgagca tctttttcaa gtttctggcg taaacgggcg atgctcaccc
     2161 ataaaatttc cttatcattt ttatattcaa caccccaaac actggtcagc aattcttcag
     2221 aagtgagaat cttgccaacg ttgtgagcaa attgaagcag tagtctatat tctgtagcag
     2281 agagagaaac tgcctcttcc ccacgccaca cttcggcacg ggcaaagtct atcttcaggt
     2341 catcatgtgt aaagaagcgc acttgccctg catcggttgg gttatgagca cggcgcagca
     2401 cagcacgaac acgagcaagc aactcagttg cagagaaagg cttgatcagg taatcatctg
     2461 ctccaaggtc tagcccacga acacgatctt gttcttcgcc gcgtgccgtc agaataacaa
     2521 taggcacatt ggtgaattca cgcaaacgct gggttgcacc aaagccatcc agcctgggca
     2581 tcatcacatc catcaacaca aggtcgatgg ggttggatga aaagacatcc agcgcttgta
     2641 agccgtcttg ggcggttact acctcatatc cttctgtacg aaggtttgcc tctaacagac
     2701 gaagatagcg gggttcatca tctacgatca gaatacgctt tgccataaga actccttgaa
     2761 gaatcgtgtt tcacttaaat gtcaaagcgt tgggtcggca ggaagctcaa tgaaaaatgt
     2821 agttcctcga tccaacactg actctaccca aatattacca tgatgtgcca taacaatttg
     2881 cttgcaaata tacagcccta aacccgtccc ggtcaccgtg cgttcaccgg gcacacgata
     2941 aaatcgctca aacaagaatg gtaggaattc ctctggaatc ccatcgcctt catcagcaaa
     3001 tgagatccgc accttcttct tttgatggct tccaccaaca atgagctttg agccgggcgc
     3061 atacttaatg gcattactaa aaagattctc aaacacctgc gaaagacgta ctccatcgcc
     3121 aaggactggc ggcaggacat ccatgttcat ttcaatcact aaattcgggt ggtgcgtctt
     3181 tactcgtgct gccacatcgc ggacaacagc atccaagcga ataggagaaa acttaaattg
     3241 gatcgtctta ctctgcaagc gagcggactc aagcatatct tcgatcagtt ttgccagacg
     3301 gtccgtctct tcatcaatga tcgacaagaa ctcacgttga gtggcgtcgt cccattgggt
     3361 gtcttgccgt aacaagctag tgctataccc cttaataaac ccaagcggag tacgtaactc
     3421 gtgcgaaatc gtagagacaa aatcatcttg caaacgcatc tggcgctgaa cagagtccag
     3481 ttcttcgcgc gcctcttgca attcttttcg ctcaattaac gccgccgccc aaatcgcctg
     3541 tagcgaagcg acatgaatat ggatatcaga atattcaggt ccaccaaagc gcacaaacaa
     3601 aagagcgcct cttaattttt cgcccacata taaaggaatc cccaacacaa aggtttgaga
     3661 catgcggtca cgatggttcg aaccttttgc gggtccatga atgaccatat gctcattcgt
     3721 gataacgtca tttgccacgc tttcgcccca agcagcgtct gcttctgcgg ttttaccacg
     3781 cccaatggca cgcgcataca caacatctaa ccctcttgtt tggcgttcac ttaaatagac
     3841 ggcaacatta tcaaacacaa aagaacgacg catggagata aacaacgcgt ccagtgctga
     3901 gcgccagtct ggttttgctt gaacatcttg aaagattcga tgaaggtcag aaagcgtctc
     3961 tttatccata ggttaatctc gcatggcaag cagagggaat ttaaacgtcg tccccttgcc
     4021 ctccgttgat ttcacgtcca gcaaagaacc atgcccttcc acaatttgac gtaccagtga
     4081 taaccccaat cctgtgccgc cgtagcgtcg cgtcgaagag ccatccaatt gatggaacgg
     4141 ctcgaagatc tctttcatac ggtcctgtgg aataccgacc cctgtatcgc gaacaatgac
     4201 ctgaactaga tttttacctt cttcttcaat agcaactgtt acgcttccgc ccgacggggt
     4261 gaacttgatt ccgttatcga ttaattgccc tactacccag ctgatcttct cgccatctgc
     4321 ttgaacatat ggcgtatcat ccttaaccaa tacttccaac ttcacaccac ggtcttctgc
     4381 tttgcgcatg gcggtctttg taaccaatga aactagccgg cgaatatcca cgccttcaag
     4441 tttcatactc atttcgccgc gagatgccaa ggagaacatg atgaggtctt cgatcatgtc
     4501 ttccagtttc cccgtagaac gctgactgac cgtcaatgca tggcgctgtt catcggagat
     4561 gggtcccagc gactctgcca acatcaattc aatatagcct ttgatgtgcg taagcggagt
     4621 tcttaattca tgcgagatgt ttgaaacaaa atttgccttc atctggctaa gctcagacag
     4681 gcgatttaat gcctcattca ggtctgaggt gcgttcacgg acgcgctgct ccaagttgcg
     4741 gtttgccgat tgtagggcag agcgcaattg aatgatctgc tccgtaatga cctgatccag
     4801 tgtgtcttta tcgatgagtt tgagttcgat caatgcctga cccaaaagag actgcccacc
     4861 tttataggtt acatcctgct gatggttcaa cgcacgttga aggtctattt cgctgatcaa
     4921 ccctttttgg acgagatact cccccaaacg cggaacgagc atctctggtg ataattttgg
     4981 cgactttgat tccatccgaa accgtccgaa tttctttatt ttttcgtacc gaatttctaa
     5041 gcaacccact ccccatctat catgacagca gaaggcgaca ttgtcaacag ggttgagggg
     5101 tcaattttaa aaatatcttc gtccaaaaca ataaggtctg catgatagtt ttcagccagt
     5161 ttgccaaggc gattctcggc attggcagtg tatgccgcgc cgaaggtata agcggacaag
     5221 gcttcggcga gggtcaattt ttgttcggcg cgccagccct cagcggaggg agagccgtct
     5281 gcccgttggc gggtgactgc ggcatgcaat ccccagaacg ggttcggtga ctccacgggt
     5341 gcatccgatc caaaggcgag gatcgcgcca tagttgagtt gagtccgcca ggcgtaagaa
     5401 agttcggcgc gtttgcccca ataacgatca gcggcaaaca tatcggaggt ggcgtgaacg
     5461 ggctgcatcg acgcgatgac gttgagcttc gcaaggcgcg gcgcatcgtc gggatggatg
     5521 acttgcacat gttcgatgcg atgacgcaga tgcggcaaat gattttgcgt ttcgtaattg
     5581 cgcaactgct caaaggcatt caacacttca tgattggctt tatcaccgat ggcatgcaca
     5641 gtcatactca aaccgacatc ggcggctttg cgagcgtgct cgaaaagttc ttcgccatcc
     5701 atgttcagaa tgccacgatt ttctttctca ccttcatagg gctggaacat cgccgccgtg
     5761 cgcggaccga gtgcgccatc cataaaggct ttgaccgagc cgatccacaa catctcatcg
     5821 ccaaagccgg tgcgcaagcc gaggtcgttc gcctgctcca cgctttcaac gggaatattt
     5881 ttacacacgc gtaattttaa tttgcctttt gctcgcaaag attgcaaagc cataaacgat
     5941 tgacggcgat caaaatcgtg gacgcccgtg atacccatct tccataaaac ggactgcgct
     6001 ttttcaaacg ccgcttccaa ctcagccaga gttggttctg gcaccgcagc attgaccaac
     6061 gccatcgccg tttcaatcat gacgcccgta gctttcccgt tcgcatcgcg ctgaacggct
     6121 ccatctttcg ggtcgggtgt ttcatccgtg atattcgcca acttcaatgc ggacgtattc
     6181 gcccatgccg catgcaatga ctttgccgtg agataaaccg ggttattcgg cgagacagca
     6241 tcgagttccg ccgcggttgg gaagtttcca tcccaatcat tctgctgcca accatgaccc
     6301 agcacccact cgcccggctt gagagtccgc gcacgttccc ccacccggcg tagacattct
     6361 tccttcgtct tggtctcgca atccactttg gaaagcccaa gcgaataaaa ctgcatatga
     6421 atgtgcgcgt cggtcagacc cggcaagata gtcttgcctt tcatatcctg cttttccacc
     6481 ttgccatgtg caaggctttc aagctggtct ttgctaccta cagcaataat ccgcccacct
     6541 gcgatgagaa tcgcagaagc atgcgaatta gcagaatcaa gggtatgaat gtttgcgtta
     6601 tagagaagtt tcatattttt gtaggtgcag ggtctcctgc ccaaagcttt acaaaggaat
     6661 aaggcgcgga aaccgcgcct ctacgaatta cgacatcttc ttaatcaatg catccaactc
     6721 ttcatcggaa taataataga tcgtcaccgt gccgcccttt ttgccatgtt tgagcgcaac
     6781 cttcgtgccc aaactgcgct ggaggcgttt ctctacatcc aacacatcgg cactgacggt
     6841 tgccttcttc actttcacag gttttacgcc cgtcaactta cggatcaatt cttcagactg
     6901 acggacgctc aacgacaaat tgataatggt ttgcagtgca gaggtttgcg ccttctgagt
     6961 ggacagcccc aataaagcac gagcgtgacc ttcggtaatg gtattgtcca ccaaggcttg
     7021 tttgaccgcg tctgcgagat tggtaaggcg catagtattg gtcaccgcca cacggctttt
     7081 gcccacacgt tttgcgatca tttcatgcga aagcccgaaa tcctccgcaa gctgatgata
     7141 ggcttcggct tcttccatgg cgttcaagtc tgcgcgttgg acgttttcga tcaacgcaat
     7201 ttcaagtaat tcctggttat tcgcctggcg cgttaccaca gggactttcg tcaacccagc
     7261 ccgctgcgaa gcctgccaac gacgttctcc cgcaataagg atgaaagtgc catctccctt
     7321 cggagagacg atcaacggtt ggatgactcc atgctcacga atggatgccg ccaagtcgtt
     7381 caggtcttcc tctttaaact gcacacgcgg ttgccgaggg ttgcgcttaa ttgcctccac
     7441 cggcacctgt tgtacaccgc cttctccact tgttttgctg ctcgtcaccg ccggagtcga
     7501 agactttccg cccggaatga gcgcatccaa acctttgcct aaaccttttc gttgagccat
     7561 gttctctacc ttttcgacca caaagtattg aagttttcct tcgtgtctct ttgtgacact
     7621 tcgtggttaa tttttatcgc ctttcaacaa ttctttcgcc aatgtttcgt aggcgtctgc
     7681 accgggagaa ttgggtgcgt attctgagat aggcaatcca taggaaggtg cttctgccaa
     7741 acgaatgtta cgcggaataa tgctcttaaa cacctgattg gggaaatgtt tattcacttc
     7801 gcgtacgaca tcggcagaaa gattggtgcg gttgtcgaac attgtcagaa tcacgccgcg
     7861 cactttgagg tccgggaaaa gagccgcgcg cacgcgttca atcgtctcgg tcaactgacc
     7921 taatccttca agcgcaagat attcgcattg caccggtacc accacaccat caattgctgc
     7981 catcaagcca ttcacggtca gcagtcccaa agaaggcggg caatcaatca acacataatc
     8041 ataacggctt gccaccttca acaacgcctt gcgcaaacga gactcgcgtg caagctcatc
     8101 caccagttct acttcggcgc cagccaaaga aggcgaagaa ggcaacagcg acaactgcaa
     8161 ccgctcattc aataagataa acggaaagat atcatcctcg ccgagcagcg catcataagt
     8221 actgccctcc acaagaagtt tatccacgcc caaacaagag gttgcgttcg cctgcgggtc
     8281 gaggtctacc accaacacac gttgccccag cttcgccaaa tacgccgcta ggttgatcgt
     8341 ggatgtggtc ttccccacac cgcctttttg attcaccaga gtatagatct tcgtcaagac
     8401 aaaacctcca tcttgcaatg ttcaatttca tcatgagtgg gaatgccctg caaacccaca
     8461 cgtgttacag aatatgccgc caaatgagtc gcaaaacgtg ctgcttccca cgggtctttt
     8521 gtttgatgta accgcgaaaa aaatccagcc gcaaaaatat cgcctgcccc cgtcgcatcc
     8581 acctcttcca ctttgatggg acgataccgt ctgcggtcgc catgccaatg caacaccgcg
     8641 ccctcttccc cttccgtcaa acacagcaga cgggcgtgat gcgccagcca ttcaccgagt
     8701 tccaaatcat ggttgatatc ctcaagcgac atcaccacgc ctcccacctg accgagaacc
     8761 tgttcgctgt tttcccaagc cgtagcgaac accgaacctt tttcgtccca gccacgcatc
     8821 cagccctgcg gcgtgactcc cacccacgaa gctgggaatt gccccaccaa agccgggtcc
     8881 acttcctgtg caatcggcgc gaggtggaca atcgccgcat ctcgccagat ctgtgggaca
     8941 tgctcaaacg aaatcttcgt cgcttgatga tgcaaggttt gtttgcgtcc gtgctcggtt
     9001 ttaacgttct caaaaatcgt gctttgttcg gttgggaggt tgaatatttg aaccccatcc
     9061 aaaaggctta agggagcatc ctcccccgct gaggtgacaa taccaacccg caagcccatc
     9121 gcctttgccg tcaacgccgc gtatgaaacc gtcccaccga gttgtttgcc cgatggagtt
     9181 aaatcgacag ccacatgacc aataagaaga tagtccactg ggcgaaggtc agacataggt
     9241 gaattatacc gtagtgaaat ctttcctctt tcttctttct tgcaagaaga aacaaaaaga
     9301 ccgtagattt tcacctacgg tcttttatct atcagtaaat gtcgaactat tctttcggca
     9361 gaatgaccac tttgcgatag ggttcttcgc cggtgctctc ggtcttcacg gcaggatgtc
     9421 cacgcagttc gatatgaacc acacggcgct cgctggaagg catcggttcc atggtcac
//
LOCUS       DAOQNJ010000030         9363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3583_length_9363_cov_8.260529, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000030 DAOQNJ010000000
VERSION     DAOQNJ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3583_length_9363_cov_8.260529"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLG52_01730"
     CDS             <1..918
                     /locus_tag="PLG52_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNB40187.1"
                     /translation="WRGEGKPILLVCHMDTVFPLGTIEKFPYQEEEGKIKGPGVSDMK
                     ASIAISLGAIEEAYNNGAKRAITLLCTSDEEIGSITSRELIQNMAKEAELVFVMEPAL
                     LDGSLKTWRKGGGGFKVKVHGRAAHAGGDHEKGRNAIEEMAHQVLAIQKMTDYSKKTT
                     LNVGVIHGGTVSNVVPDYAEIDIDVRILQPEEWGRVEGMMMALKPVLDGTTVEVTGSL
                     NRGPMPFDELMQATFGKAKSIAAEIGIDLKAGGTGGVSDANLVAPLGIPVLDGLGAIG
                     EGYHSEREYIFAGSVAQRVKLISALLVNW"
     gene            1063..1572
                     /locus_tag="PLG52_01735"
     CDS             1063..1572
                     /locus_tag="PLG52_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40188.1"
                     /translation="MNDLFTCADELSLAVKVAADSPDIYLRIGEPGDVLTPVFQIDEE
                     ELLIVTHRESPVQNLSLEETQALFAGSSGLAVQVWVYPSELDVYEVFDQFVMQGRSVI
                     SAARVAVNPREMSDVLNQESNAVGILPRHWKAGNVREVYSVGIFPVLAVTQEEPQGNV
                     AQLIACLQK"
     gene            1669..2319
                     /locus_tag="PLG52_01740"
     CDS             1669..2319
                     /locus_tag="PLG52_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40189.1"
                     /translation="MNQDFNEIQQRVRHSWFKDGVGEIAVGGLFVALSLYFAGHYFAG
                     TRFLPSASNAPLYIDLGLGVVLLLGIVLTRKIIAVFKTHITYPRTGYVEYYIESKNSL
                     LVRVAIFIAGTLFVLLLIWVGRWVGSFKWIPAFLGVFFGGVLLFLRTKTAGLKRFNYH
                     ASLSILLGLGLTFSSMPIPGLSVYYAVFGVWLLGWGIWILAKYLRENPLPEGDEDE"
     gene            2312..2623
                     /locus_tag="PLG52_01745"
     CDS             2312..2623
                     /locus_tag="PLG52_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNB40190.1"
                     /translation="MNDDLRSLTDLDRMIHEPARLLLVTILSGVASADFLFLQRQTGL
                     TKGNLSAHLSRLEEAGYVKIEKTFKGKMPFTVCKLTSAGQKALQRYRQQVQNFMDKTR
                     S"
     gene            2696..3430
                     /locus_tag="PLG52_01750"
     CDS             2696..3430
                     /locus_tag="PLG52_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40191.1"
                     /translation="MNTPLIELKNVSKTYSTAAGEFPALRNINLNVYAGEFLGVIGKS
                     GAGKSTLLNMINGVDELSEGEVIVNSNNSRVSVHNLSEDEKALWRGKTMGVVYQSFQL
                     LPMLTLVENITLAMDLADNFKPRESRERAMELLRLVEIEEHADKLPSLISGGQQQRVA
                     IARALANDPDILVADEPTGSLDSVTADHIFDVFSRLANERGKTIVMVTHDMGLKSRFS
                     RCLTLVDGELQNEIKADAKPKRSRKQ"
     gene            3427..4620
                     /locus_tag="PLG52_01755"
     CDS             3427..4620
                     /locus_tag="PLG52_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB40192.1"
                     /translation="MTQPKKKRIPSHAGSPRTQPKAAPQKVHDASPEAIIEMRGVVKK
                     FSNAAGEFTVLKGVDLMINRGEFVSIVGKSGSGKSTLLNMITGIDHPTSGQVIVNHND
                     IYTGVSESARSKWRGKNLGIVFQFFQLLPMLTLLENVMLPMDYVDMYDFDERPIRALE
                     LLELVGLEKFANKLPVLVSTGQQQLAAIARAMACDPPLLIADEPTGNLDTRSSDTIIK
                     LFQHFVDQGKTIVMVTHDPSLTSRTDRNIIISDGEIIDETISRSLPQLRHRHMLEFSK
                     LAQRRMYQPRETIISRDEPVKNFFMIRSGQVEVVLQKQRSKETVLSRLGPNEFFGDIE
                     LLRGGKSIANVRAGSEPVEVVIIPREDFTRVMDESPITVEAISKIVQRRLEQHRMVDP
                     RKSRS"
     gene            4631..>5550
                     /locus_tag="PLG52_01760"
     CDS             4631..>5550
                     /locus_tag="PLG52_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB40193.1"
                     /translation="MNADPRRLPQVLYIADSDESRSLVRRLLAYTFVVLEAANPLDGL
                     GLAEETRPNLILLDTNVAQMTSSEAATRLKKMLSGTPIVVVSGDTSNKARARALAAGA
                     VGFIPKPIGDDFEQLVIEYLNGRTDELENAEQYLREYQLELAEKIEDQTRQLTNTVER
                     NKYLLSQNQKMFAMLERRHKLLETAARVGQMVTSILDMTELLRHTVNIICSEFHFYYS
                     GIFLISDDRKWAVLRAGFALAGRKMLDADYKLPIDDKSMIGRSILTQQAQIALDVAGE
                     ESRFKNPYLPNTRSEMALPLIVNSISVGAVT"
     assembly_gap    5551..5650
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5651..6388
                     /locus_tag="PLG52_01765"
     CDS             <5651..6388
                     /locus_tag="PLG52_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB40194.1"
                     /translation="AQLKESELDNAALQAAVETVREEAEAAGLDLKNILAEMSAMKPN
                     RLQALVSVESLMEDLDIAEHSAKTILDIKEGLIGPARQRHDAPVSLSEMITNTVDNMG
                     LPHGAVEMKWAEDMPMAHVDARQVEQVFNNLIKNAWEALTLSNIPEPKIYVDGRCDKD
                     ANFVLVSVKDNGPGIPKEIQEKIWVSFFTTKGGKGGTGLGLSAVMQIVNQHGGKIWLE
                     SEPGQGATFYVSLPAEKLYMPDENTSR"
     gene            6410..6799
                     /locus_tag="PLG52_01770"
     CDS             6410..6799
                     /locus_tag="PLG52_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB40195.1"
                     /translation="MMTTVLLVDDEKLIQRSLEKTLLRAGYDVLTAPDCKTGSEVFAQ
                     NASTVDIAVLDLNMPGFDGMPKNDAGLDLLADLKQKKSTLPVVILTAYDEVNKAKDAV
                     SKGANAFFVKGREQGLVELIQKILSGN"
     gene            6822..8891
                     /locus_tag="PLG52_01775"
     CDS             6822..8891
                     /locus_tag="PLG52_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB40196.1"
                     /translation="MTNNIERHAKLLKASARAAKNITTILDPYELFQRTVDIICDEFG
                     FYYAGVFLVDDASQYAVLKAGRGDAGKAMLREEHKLAIGGNSMIGASIANKKGRIALD
                     VGEEAVFFENPHLPKTRSEMALPLIIADEVIGALTVQSTEEAAFHEEDIDALQTMADQ
                     LAVAIQNSKLHRQEERRSRLLKAANRVGKEVASLLDLDQLLPQTVNIICEAYGLYYAG
                     VFLLDEKGEYAVLRAGYGKAGKAMLSEGHKLRVGTDSMIGACVAMGEARIALDVGEER
                     VHFKNPHLPHTRSEMALPLIYGGKTLGAVTIQSSEERAFSDDDITTLLTMAEHLAVAI
                     NNAKLIDELKEAHNEILRNKVFEALTSASTEAIHWIGNKTLPISLTVSRLREEIADGK
                     VDVESLKEDLDMISESAAQIIQVKEQLIGAVREMKPRPVLFADVLQTAAMQRGIAQNA
                     LKVSIDPEAAYVIADSTQLTRAVGNILQNAVEAGAKSVKVRVHPTEERGVLEINIEDN
                     GSGMNDETQRKVWSPFYTTRGVSHHGLGLPAAMHVVSQSQGRIALVSEEGKGTTVALF
                     MPQGRVTSSEVQPGGVKNILLIDDNDDWSNLLTEMFKGSKVKLTQTTDLKKFPEADLI
                     LVDENIASAPLTDVLAALSKAGLASKTVVLTSAINPERVTQYLREGLKDVTVKPYSVN
                     ELNELLK"
     gene            8945..>9363
                     /locus_tag="PLG52_01780"
     CDS             8945..>9363
                     /locus_tag="PLG52_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40197.1"
                     /translation="MRPRWRKVFHDLWDNFMRTALVVLSIAVGVFSIGVITGAYEIIS
                     NDMGESYAANNPANIELRMTNFNDDVLSSIRNFKGVQDAEGRRVINYRVHPEGSTQWS
                     TIDLIAVEDFSKNNVNLLVPILGKAEAGKDEIVLNKK"
BASE COUNT         2427 a         1977 c         2385 g         2474 t
ORIGIN      
        1 tggagagggg aaggaaaacc cattttgttg gtctgccata tggatacagt ttttccatta
       61 gggacgattg aaaaatttcc gtatcaagag gaagaaggca agatcaaagg accgggcgtc
      121 tctgatatga aagccagcat tgccatttct cttggtgcaa tcgaagaggc atataacaat
      181 ggagcaaaac gagctattac gttgctgtgt acttcagatg aagagattgg cagtattacg
      241 tctcgcgaac taattcaaaa catggcaaag gaagctgaac tcgtttttgt gatggaacct
      301 gctttgctgg atggttctct caagacttgg cgtaaagggg gcggcggatt taaggtcaag
      361 gttcatggac gggctgcgca tgcaggcggt gaccatgaaa aagggcggaa tgcgattgaa
      421 gaaatggcac atcaagtact tgctattcaa aagatgacgg attactccaa aaagaccaca
      481 ttgaatgtgg gggtcatcca cggtgggaca gtttcgaatg tcgtgccaga ttatgcggag
      541 attgatattg atgtccgcat tttgcaaccg gaagagtggg gacgggtgga aggaatgatg
      601 atggctttga aaccagtcct ggatggcaca actgtggaag tgacagggtc actcaaccgt
      661 ggaccaatgc cattcgatga gttaatgcag gctacctttg gaaaagccaa gtccattgct
      721 gctgagatcg gcattgacct caaagcgggt ggtacaggcg gcgtctctga tgcgaatttg
      781 gtcgcgccat taggaattcc ggtcttagat ggtcttggtg cgattggcga aggctatcat
      841 tcagagcgcg aatacatttt tgcaggtagt gtggctcaac gtgtaaagtt gatctcggca
      901 ttgcttgtca attggtaatc attttctatg gctgttccat atagcaagaa gattgttttt
      961 gtttttcttt ttctttcctt ttttttgttt tcctgtgcac caaaaccatt atcttcaaat
     1021 cagattatca cagcgtattc cacagcatct gcacagccat ggatgaatga cttatttacc
     1081 tgtgcggatg aactgtctct tgcggtgaaa gttgccgcag actcgcccga tatttatttg
     1141 cgtatcggcg agccaggcga tgtactaacc cctgtatttc aaattgatga agaagaacta
     1201 ctcattgtca cgcatcgtga aagcccggtg caaaaccttt ctttagaaga aactcaagct
     1261 ttatttgctg gttcaagtgg attggcggta caggtttggg tgtatccatc agagttggat
     1321 gtgtatgagg tgtttgacca gttcgtgatg caaggacgaa gcgtgatatc tgcggctcgg
     1381 gtggcggtga atccgcgaga gatgtctgat gtgttaaatc aagaatcgaa tgcggtcgga
     1441 atcctgccgc gacattggaa agcggggaat gtgcgtgagg tgtattctgt ggggattttt
     1501 cccgttcttg ctgtgactca agaagagccg caggggaatg ttgctcaatt aatcgcttgt
     1561 ttgcagaaat aaaattctca tcttagtttt atttttcact tgacgcctcc taatgggatg
     1621 gtattatcta aaccggtttg tagtgcaaac cggtttgtgt ggagtgttat gaatcaagac
     1681 tttaacgaaa ttcaacagcg tgtgcggcat tcctggttta aggatggtgt gggcgaaatt
     1741 gcagtgggcg gattatttgt agcactttca ctatattttg ccgggcacta ctttgctggc
     1801 acacgctttt tgccttcggc ttccaatgcg ccgttgtaca ttgaccttgg tttgggagtt
     1861 gttcttcttt taggcatcgt gctaacacgc aagatcatcg ctgtgttcaa gactcatatt
     1921 acgtatccac gtacagggta tgtagaatac tacattgaat ctaagaactc tttattggta
     1981 cgagttgcta tttttatcgc aggtacatta tttgttctgt tattgatttg ggttggaaga
     2041 tgggtggggt ctttcaagtg gattcccgct tttctgggcg ttttctttgg cggagtcttg
     2101 ttatttctgc gcacaaagac agctggcttg aaacgtttta actatcatgc aagtttgtcc
     2161 atcttattag gacttggact aacatttagc agtatgccta tccctgggtt gagtgtgtac
     2221 tacgctgttt ttggcgtttg gctgttaggt tgggggattt ggatcttggc aaagtatttg
     2281 cgtgagaacc cactgccaga gggagatgaa gatgaatgat gacctccgtt cactaaccga
     2341 tctagatcgt atgattcacg aaccggctcg gctcttattg gtaaccattc tctccggcgt
     2401 tgccagcgcc gattttctct ttctccaacg ccagaccggt ttgaccaaag gaaacctgtc
     2461 tgcccatctt tcgcgtttgg aagaagccgg gtatgtgaag atcgaaaaga cctttaaagg
     2521 aaaaatgccc ttcacagttt gtaagctgac cagtgctggg caaaaggcgt tacagagata
     2581 tcgccaacag gtacaaaatt tcatggataa gacccgttcg tgagcaattg aaaactcttt
     2641 gcaaataaag gacacaaaat cgcatcaaca acagcggtaa aatgaaattg aacttatgaa
     2701 tacaccattg attgaattga agaatgtatc aaaaacatat tctacggcgg caggcgaatt
     2761 ccccgctttg cggaatatca acctaaacgt ctatgcgggc gaatttctag gggtaattgg
     2821 taaatcaggc gcgggaaaat ctaccttgtt gaacatgatc aacggagtag atgaactgag
     2881 cgaaggcgaa gtgatcgtca actcgaacaa ttctcgtgtt tctgtgcata acctgagtga
     2941 agatgaaaaa gcactctggc gcggtaaaac tatgggcgtt gtgtaccagt cctttcagct
     3001 tctacccatg ctaaccctgg tcgagaatat cacccttgct atggatctgg cagataattt
     3061 caaaccccgc gaatcgcgtg aacgcgctat ggaacttttg cgtttggtag agatcgaaga
     3121 acatgccgat aaactcccat ccctcatctc gggtggtcag cagcaacgtg ttgcaattgc
     3181 gcgcgcattg gcgaacgacc ccgatattct tgttgccgat gaacccaccg gcagcctcga
     3241 ctctgtcacc gcagaccaca tctttgatgt cttttcgcgt ctggcaaatg aacgtggcaa
     3301 gaccattgtg atggtaacgc atgacatggg cttaaagagc cgcttttctc gttgcttgac
     3361 tctcgtcgat ggtgaattac aaaatgaaat caaagcggat gccaaaccga agaggagccg
     3421 caaacaatga cgcaaccgaa gaagaaacgc attccctcgc atgccgggtc gcctcggact
     3481 cagccgaaag ctgctccaca aaaagtgcat gatgcctcac cggaagcgat catcgaaatg
     3541 cgcggcgtgg ttaagaaatt ttccaatgcc gctggtgagt ttaccgtcct caaaggcgta
     3601 gacttgatga ttaaccgcgg tgagtttgtg tctattgtgg gtaaatctgg cagcggtaaa
     3661 tcgacgctat taaatatgat cacaggcatt gaccatccta ccagcggaca agtcattgtc
     3721 aaccacaatg atatttatac cggcgtgagt gagagcgctc gctcaaaatg gcgcggaaag
     3781 aacttgggca ttgttttcca attcttccaa ttactgccca tgttgacctt actcgaaaac
     3841 gtcatgcttc cgatggatta tgtggacatg tacgattttg atgaacgtcc tatccgcgct
     3901 ttggagcttt tggaattagt aggtttagag aaattcgcta ataaactacc ggttttggtt
     3961 tcaacgggtc aacaacagtt agcagcgatt gcacgtgcca tggcatgtga ccctccgcta
     4021 ctgattgcag atgaacccac cggcaacctc gatactcgtt cttctgatac catcatcaaa
     4081 ttgtttcagc attttgtcga ccaaggcaag accattgtga tggtgaccca tgacccatcc
     4141 ttaacctcgc gtacagaccg taacatcatc atctctgacg gcgaaattat tgacgaaacg
     4201 atttcccgct ctttgccgca acttcggcat cgccacatgc tggagttttc caaacttgcg
     4261 cagcgccgaa tgtatcagcc gagagaaacc attatttctc gcgatgagcc cgtgaaaaac
     4321 tttttcatga tccgtagcgg acaagtagaa gttgttttac aaaaacaacg cagcaaagaa
     4381 accgtccttt cgcgtttggg acctaatgaa ttttttggtg atattgaact cttacgcggc
     4441 ggcaaatcga ttgcgaatgt acgtgcagga agcgagccgg ttgaggtcgt gatcattcca
     4501 cgtgaagatt tcacgcgtgt tatggatgaa tctcctatca ccgtagaagc catcagcaag
     4561 atcgttcaga gacgtttgga acaacatcgc atggtagacc caagaaagag tcgctcgtag
     4621 atttgctcct atgaacgctg accctcgcag actcccgcaa gttttatata tcgccgatag
     4681 cgacgaatct cgttcgcttg tgcggcgttt gcttgcatat acctttgttg tgttagaggc
     4741 tgcgaatccg ttagatgggt taggactggc tgaagaaacc cgtccaaatt tgattttgct
     4801 agataccaat gtcgctcaga tgacaagcag tgaggcagca actcgcctta agaaaatgtt
     4861 gtctggtact cccattgtgg ttgtttctgg agatacatcg aataaggcac gtgcgcgggc
     4921 tttggcggct ggagcggtgg gcttcattcc caagccaatt ggcgatgact ttgaacaact
     4981 ggttattgaa tacctgaacg gcagaaccga tgaattagaa aatgccgaac agtatttacg
     5041 tgaatatcaa cttgaattag ctgaaaagat tgaagaccaa actcgtcaac taaccaatac
     5101 ggttgaacgc aataaatacc tgctttcgca gaaccagaag atgtttgcca tgcttgagcg
     5161 cagacataaa ttgttggaaa cggcggcacg agtagggcag atggtcacat ctattctgga
     5221 tatgacagaa ctacttcggc acaccgtcaa tattatttgt agtgagttcc atttctatta
     5281 ttctggaatt ttcctgatct cagacgaccg taagtgggcg gttctacgag cagggtttgc
     5341 attggctggg cgcaagatgc tggacgccga ttacaaattg ccgattgatg ataaatccat
     5401 gatcggacgc tcgatcttga cacaacaagc tcagattgca ttggatgtgg cgggtgagga
     5461 gtcacgcttc aagaacccgt atttgccgaa cactcgctca gagatggcat tgccacttat
     5521 tgtcaattcg atctctgtcg gtgcggtgac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn gcgcagttaa aggaatcaga gttggataat gccgcgttgc aagccgccgt
     5701 tgaaacggtc cgggaagagg cggaagcggc aggcttggat ttgaaaaata ttctggcaga
     5761 aatgtctgcc atgaagccga atcgcttgca ggcgttggtc agcgttgagt ctttgatgga
     5821 agatttagac attgccgaac atagcgcaaa gaccatcctt gatatcaaag aaggtttgat
     5881 cggacctgct cgtcagcgac atgatgcgcc ggtttctttg agcgagatga tcacgaacac
     5941 ggtggacaat atgggcttgc cgcatggcgc cgtggaaatg aaatgggcag aagatatgcc
     6001 catggcacat gtggatgcgc gccaggtgga acaggttttc aataacctca tcaaaaatgc
     6061 ttgggaagcg ttgacgctct caaatatacc ggagcctaaa atttatgttg atgggcgctg
     6121 tgataaagat gcaaattttg tgctcgtttc tgtaaaagat aatggaccgg gaattccgaa
     6181 agagatacag gagaaaattt gggtgtcgtt ctttacaacc aaaggcggta aaggtggcac
     6241 ggggttgggg ctttcggcag tgatgcagat tgtcaatcaa catggtggca agatttggtt
     6301 ggaaagtgaa cccgggcaag gtgctacatt ttatgtgagc ctgcctgcgg aaaagttata
     6361 tatgccagat gagaatactt cacgctaatg cgtgtaaaaa cttggaggaa tgatgacgac
     6421 agttctttta gtggacgatg agaagttgat ccaacgcagt ttggaaaaga ctctcttgcg
     6481 ggctgggtat gatgtgttga ctgcgcccga ttgtaagaca ggcagtgagg tctttgctca
     6541 aaacgccagc accgttgata ttgccgtgct ggatttgaat atgcccggtt ttgatggtat
     6601 gcctaaaaat gatgctgggc tcgacctttt ggcagatctc aagcaaaaga aatctacatt
     6661 accggttgtg attttgacgg catacgatga agtgaacaaa gccaaagatg ctgtttctaa
     6721 aggtgccaat gcctttttcg taaaaggacg tgagcaaggt ttggtggagt tgatccaaaa
     6781 aatcttgagt ggaaattaat tcactttcaa cagaggaact tatgacaaat aatattgaac
     6841 gacatgcaaa attattgaaa gcctccgcgc gtgccgcaaa gaacatcacc accattcttg
     6901 acccctacga gcttttccag cgtacagtgg atatcatctg cgatgagttc ggcttttact
     6961 atgccggtgt ctttttggta gatgatgcca gtcagtatgc tgtgctcaaa gctgggcgtg
     7021 gcgatgctgg taaagccatg ctgcgcgaag aacataaact ggcaattggc ggcaattcta
     7081 tgatcggtgc cagcatcgcg aataagaaag gtcgcatcgc gctggatgtg ggcgaagaag
     7141 cagtattttt tgaaaacccg catttgccca agacccgttc tgagatggca cttccgctca
     7201 tcattgccga tgaagtgatc ggtgcgctta cggttcagtc taccgaagag gctgctttcc
     7261 acgaagaaga tattgacgca ctgcagacca tggcagatca gcttgcggtt gctattcaaa
     7321 actccaagtt gcaccgccaa gaagaacgcc gctctcgctt gctcaaggct gcgaatcgag
     7381 ttggaaaaga agttgcatct cttctcgacc tcgaccagct cttaccgcag accgtgaata
     7441 ttatttgtga agcctatggc ttgtactatg ctggcgtgtt cttgctcgat gaaaaaggtg
     7501 aatatgctgt cttacgtgca ggttatggca aggctggcaa agccatgctt tctgaaggtc
     7561 ataagttgcg cgtcggtacc gactcgatga tcggtgcttg tgtcgcaatg ggcgaggcac
     7621 gtattgcgtt ggatgtaggc gaagaacggg tgcatttcaa gaatccgcat ttgccgcaca
     7681 ctcgctctga aatggcgctt ccgctgattt atggtggcaa aactctcggg gcggtcacca
     7741 ttcaatcttc tgaagaacgt gctttcagtg atgatgacat tactactctg ttgacgatgg
     7801 ctgaacactt ggctgtcgca atcaataatg ccaaactgat cgacgaactt aaggaagccc
     7861 ataacgagat ccttcgcaac aaagtctttg aagcgctcac ttccgcttct accgaagcca
     7921 ttcactggat cggcaataag acgcttccca tctcgctgac agtttcccgc ttgcgcgaag
     7981 aaattgccga tggcaaagtg gatgtcgaat cgctcaaaga agatctcgac atgatctctg
     8041 agagtgctgc acagatcatt caggtgaagg aacaactgat cggtgccgtc cgtgagatga
     8101 aaccgcgccc ggttctcttt gccgatgtgc ttcaaaccgc agctatgcaa cgcggcattg
     8161 cccagaacgc tttgaaggta tccattgacc ctgaggctgc ttatgttatt gcagactcca
     8221 ctcagcttac ccgtgcagtt ggcaacattt tacaaaacgc tgttgaagcg ggtgccaagt
     8281 ccgtcaaagt gcgggtgcat cctaccgaag agcgcggcgt gcttgaaatt aacatcgaag
     8341 ataatggttc tggcatgaac gatgaaactc agcgcaaagt ttggtcgcct ttctacacga
     8401 cacgtggtgt ttcacatcat ggtttggggt tgcctgcggc aatgcatgtc gtttcgcaat
     8461 cacagggtcg cattgcactc gtcagcgaag aaggcaaggg aacaaccgtc gcgttgttta
     8521 tgccccaggg acgtgtgacc agttccgagg tccagccggg aggcgtgaag aatatcctgt
     8581 tgatcgatga taacgacgat tggtcaaatc tgctcacaga gatgttcaaa ggttcgaaag
     8641 tcaagttgac ccaaacgaca gacttgaaga aattccctga agccgatctg attttggtgg
     8701 atgagaacat cgcctcggct ccgctgaccg atgtgttggc tgctctttcc aaggctgggt
     8761 tagcatcgaa gaccgtggtc ctcacctctg ccatcaaccc tgagcgtgtg acccaatatc
     8821 tgcgcgaagg tttgaaagat gtaacggtca agccgtattc tgtaaacgaa ttgaatgaac
     8881 tgttgaagta aaggaaagaa agttgacagg tcttcaagac ctgtcaactt tgtaaaagag
     8941 aaacatgaga ccacgttggc gtaaagtatt tcacgattta tgggataact tcatgcgtac
     9001 tgcattggtg gttttatcca ttgcagtggg tgtgttttcc attggggtaa tcactggtgc
     9061 gtatgagatt atctcaaacg atatgggaga atcctacgcg gcaaataacc ctgcgaatat
     9121 tgaacttcgc atgacgaatt ttaatgacga tgtactgtcg tctattcgta atttcaaggg
     9181 tgttcaagat gctgaaggca ggcgtgtgat taactatcgc gtccatcctg agggcagtac
     9241 tcaatggtcg accattgact tgattgcggt agaagacttc agtaagaata atgttaattt
     9301 attggtgccc attttaggca aggcagaagc gggcaaggat gagattgtct taaacaagaa
     9361 gaa
//
LOCUS       DAOQNJ010000031         9076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3782_length_9076_cov_12.371356, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000031 DAOQNJ010000000
VERSION     DAOQNJ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3782_length_9076_cov_12.371356"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="PLG52_01785"
     CDS             <1..153
                     /locus_tag="PLG52_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40198.1"
                     /translation="MLGAERNQVFTAPHLVFYPGFMIMLTVLAFNLIGDGLRDALDPR
                     LAHSSG"
     gene            complement(204..1004)
                     /locus_tag="PLG52_01790"
     CDS             complement(204..1004)
                     /locus_tag="PLG52_01790"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB40199.1"
                     /translation="MIQPPASYSDPSLGQQPEPIPTRPPTYVRMPTLQPIVTYALIGV
                     TIFVYLLQVLSSVLFGDSSYGIDWVTLYGARISDAIYAGQLWRLFTPMFLHGSIPHIF
                     FNMYALFSVGSLLERHFGHGRFLLLYVLGGFAGNVLSFLFTQGYSVGASTAVFGLIAA
                     EAIFFYQNQKLFGSHAKQAISNAIFIIAINLFLGLAPSIDTWGHIGGLLGGAMFAWFA
                     GPKWTVVGMPPEYHLDDERESREIITGALLVALIFGGLTVWAFIQHAV"
     gene            complement(1001..1615)
                     /locus_tag="PLG52_01795"
     CDS             complement(1001..1615)
                     /locus_tag="PLG52_01795"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNB40200.1"
                     /translation="MTEFELRKPNPLLIVISGPSGVGKDSVVQRMKDRGFPFHFVVTA
                     TTREKRANETHGVDYFFLSKDEFAHMIEEDELIEYAIVYGDYKGIPKEQVRNAFASGK
                     DVVMRIDVQGAESVRKLAPEALLIFITCESEEELERRLRERKTESPDSLSLRIATARK
                     ELQRLEAFDYVIVNHDFHLDDTVDKVRAIIDAEHLKVHHRMVTL"
     gene            1675..2058
                     /locus_tag="PLG52_01800"
     CDS             1675..2058
                     /locus_tag="PLG52_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNB40201.1"
                     /translation="MPTYDFICNTCEKRFEVFLTYSEYGKKTVECTHCNSTDVRRRMT
                     KVRIAKTDEGRLEHMAGDLGSLEGMENDPAAFARRMKKMGKEMGEELPAEFDEVVDRL
                     EAGQSPEEIESAMPDLSGGDELSKE"
     gene            2282..4438
                     /locus_tag="PLG52_01805"
     CDS             2282..4438
                     /locus_tag="PLG52_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40202.1"
                     /translation="MSLKKFLVLLGVLVVLGAVLAACGGTQPTQAGTETATEEAPAAP
                     IPDTPYLADWQGSAHANVADEPFRHWDDAAENPDGVPTSCAKCHTTDGYRDFLGADGS
                     EAGKVDVAVAADHAQGVQCVACHNAVTMTKTTVMFPSGVEITAGDDSRCMECHQGRES
                     KVSVDAQIEKFAATDPDAVVAPIKDDQGNDVFFGFRNVHYYAAAATLYGGMTHGGYEY
                     DGLTYDAKNTHTDGYDSCTGCHDPHTLEVKVDQCAFCHEDVATVEDLKNIRMVSSAPD
                     YDGDGNVEEGMYYEIEGLQAALLAEIQTYAADKGGAAIIYDSASYPYWFIDTNANGAA
                     DEGEVAFPNAYSTWTPRLLKAAYNYQVSMKDPGAFAHGNKYIVQLLFDSIADLGGDTS
                     KLARTDAGHFAGNTEPFRHWDKDEEGNPVYEVEAACAKCHSAAGLPQFLEYGANLPVE
                     SANGFMCSTCHNEEAWPERYAVASVTFPSGSTVSFGGKDADGNFVADDSNLCIECHQG
                     RSSTPTLNKALTGKEEDTPDPKIRFSNIHYFAAGATIFGADVQGAYMYEGKEYVGQNM
                     HADEAGKMNKCQDCHDVHALEPKVEACETCHDTTDPTAVRESDVDYDGDGDVTEGIKG
                     EIDTMTEALYAAIQAYAEGHGGAIEYKATAYPYFFGADGKGYAAWTPRLLKAAFNYQY
                     VQKDPGAYVHNPKFVIQFLYDSIQDLGGDVSAFTRP"
     gene            4632..5798
                     /locus_tag="PLG52_01810"
     CDS             4632..5798
                     /locus_tag="PLG52_01810"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNB40203.1"
                     /translation="MHQRIKLRTPHIWTVSLLLGLGLFAFALAALTTTAQAAPSLQPA
                     AQDQKPNNDFCLACHQEEGIDKTLGSESLPVTINPTEFGLSVHAEEGVLCVDCHVDIS
                     DYPHPDVKSKSVRDFQMTFADSCKECHEDQFTDAHDSVHQTAMDEGDPNAPLCADCHN
                     PHTQGRIIGETSGELTIAARKEVPATCAKCHTETVEIYKNSVHGSALADGNTDVPTCT
                     DCHGVHNIGDPTTASFHNNTPALCADCHDNEELMGKYGLSTEVLETYVADFHGTTTTL
                     FEQQFPGQPINTAVCTDCHGVHDIASTKNPETGIAMQENLLVKCQRCHPDATANFSAA
                     WMGHYQPSPEHYPIVYYVNLFYKFFIPAVLGGMVLFILTDIYRRIINRAKGAKH"
     gene            5798..6652
                     /locus_tag="PLG52_01815"
     CDS             5798..6652
                     /locus_tag="PLG52_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNB40204.1"
                     /translation="MNTVTKAYLRFPIARRIEHWVMMLSFTLLGLTGLPQRFPEAGIS
                     QAILGVLGGIQGVRTIHHTSAIVMMFGTAWHILVMGYSVFVLRDKMSMLPTLQDAKDG
                     LQALLYNIGFAKTYPQMGRYTFEEKMEYWAFVWGALVMGATGFMMWNPITATKFLPGE
                     FVPAAKAAHGNEAVLAVLAIIIWHMYGVHIKRLNKAMFNGMQTEAEMLHEHPLELADI
                     KAGIADRRPDAATLRKRQMIYYPIAAVLTIGMLSGIYGFINAEETALTTIPPIVETVE
                     PPAPTVQP"
     gene            complement(6714..7979)
                     /gene="nrfD"
                     /locus_tag="PLG52_01820"
     CDS             complement(6714..7979)
                     /gene="nrfD"
                     /locus_tag="PLG52_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNB40205.1"
                     /translation="MKTILKTIRENIFTPWGLWLAFLGLLLVSALVAALTVFWKGLVI
                     TNLTNLVPWGLWITIDLSSIAVAAGAFSLCAIVYLFGFKRYEPIARTATFIGFTGYSM
                     AMMALLLDIGRPERFWKSFVFWNPHSLLWEVTMCVGLYFTVLIFEVLPIVASFEWLRT
                     KYPKIAEKMEHMHHYAPVLAVLGLALSSLHQSSLGAVYGVIKARPIWYRPEMSVMFMF
                     TAILGGVSLTLLASMLAARITPKAKVNDTLIERVAQVLGYMMIAYLYWRFWDWLAQTY
                     TYEPGRTEGFRLLTKGPLSFNFWFGEMLFGALFPTIILLKRSLRQTPLWRMLAFAMIV
                     GGVVAFRFDTNIVGFLAVIPYTPGEMIVQYTSYTPSLVEWAAGLGIIAFGLLAFSVGV
                     KYLRVVDHRLVSEEYEVITMKVGEAVTGD"
     gene            complement(7981..8757)
                     /locus_tag="PLG52_01825"
     CDS             complement(7981..8757)
                     /locus_tag="PLG52_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB40206.1"
                     /translation="MAQNKFNRRDFIKLSAVGIAAAAGTRLLTEEAKASEGVKGEHQW
                     VMVIDQSKCTGCNQCNMACHATNDINPEMEWTKVLDMGEMNGKQVYLPRPCMQCEHAP
                     CVEVCPVGASYYRDDGIVMMDYDKCIGCRYCMVACPYDARSFNWEKFDGENPYVPQYG
                     EPEVERRPRGVPEKCAFCYQRIDRGLSLGLKPGEDPAATPACVVACPTGARIFGDLND
                     PNSNVSQLLKDHPSYRLRENLGTKPRVYYLPVNDDTTVEG"
     gene            complement(8744..>9076)
                     /locus_tag="PLG52_01830"
     CDS             complement(8744..>9076)
                     /locus_tag="PLG52_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40207.1"
                     /translation="VHQVADHSFKASITTCNSCHANQMHTDGQAEATEQAIVASVAPT
                     ETPKPAAATVDQSPIVSPDPAPVSPLGYAGITALIGIAAGMLLAPWLERGYRLVLKKS
                     AEVHHGSK"
BASE COUNT         2314 a         2352 c         2294 g         2116 t
ORIGIN      
        1 atgctcggtg cagaacgcaa tcaagtcttt accgcgccgc accttgtgtt ctaccccggc
       61 tttatgatta tgcttacggt gttggcattc aacttgatcg gtgatggtct gcgtgatgcg
      121 cttgacccac gccttgcaca ctctagcgga taaaacattg aatcaaaaaa gcccgtcgtt
      181 aagacgggct tttttattat ttgttacaca gcatgttgaa tgaatgccca aacggtcagc
      241 ccgccgaaga tgagcgcaac aagcagtgcg ccagtgatga tctctcgact ttctcgttcg
      301 tcatccagat gatattcagg cggcataccg accaccgtcc acttgggacc tgcaaaccag
      361 gcgaacattg ccccgccgag tagaccccca atgtgccccc acgtatcaat gctgggagcc
      421 aagccgagga agagattgat ggcgataatg aagatggcat tgctaatggc ttgcttggca
      481 tgtgaaccga ataatttttg gttttggtaa aagaagatgg cttctgcggc aatgagacca
      541 aataccgctg tggaagcacc gacggaatag ccttgggtaa agagaaaaga caatacattc
      601 ccggcaaaac caccgagaac atacagcaac aagaagcgcc catgcccgaa gtgccgttct
      661 agcaaactcc ccaccgagaa aagcgcatac atattaaaga aaatatgcgg gatggagcca
      721 tgcaggaaca tgggcgtgaa caaacgccaa agttgcccgg catagattgc gtcactgatg
      781 cgggcgccat ataaagtcac ccaatcaatg ccgtagctag agtcaccaaa aaggacagaa
      841 ctgagaactt gtagaagata aacgaaaatg gtcacaccaa tgagtgcata ggtgactatg
      901 ggttgcaagg tgggcatccg cacgtaagtg ggcggacggg taggaatcgg ttcgggttgt
      961 tgacccagtg acgggtccga ataagaggcg ggaggttgaa tcacaaggtc accatgcggt
     1021 ggtgcacttt caaatgttcg gcatcaatga tcgcgcggac tttatccaca gtgtcatcca
     1081 aatgaaaatc atgattgacg atcacatagt cgaaggcttc gagccgttgc agttctttgc
     1141 gggcggtggc aatgcgtaga gaaagggagt ctggcgattc ggtcttgcgt tcgcgcaaac
     1201 gtctttctaa ttcttcttcg ctttcgcacg tgatgaaaat gagcaaggct tcaggcgcaa
     1261 gcttgcgaac cgactcagct ccttgcacat cgatgcgcat gaccacatct ttgccgctgg
     1321 cgaaggcatt gcgcacctgt tctttgggaa tgcctttgta atcgccatag acgatggcat
     1381 attcgatcaa ctcatcttct tcgatcatgt gcgcaaattc atcttttgag aggaagaaat
     1441 aatccacgcc atgggtctca ttggctcgct tctcgcgagt ggtggcagtc accacaaagt
     1501 ggaagggaaa cccgcggtct ttcatgcgtt gcaccacaga gtctttgcct acaccagaag
     1561 gaccagagat gacgatcaac agcggattgg gtttacgaag ctcgaattca gtcatctgcg
     1621 tattgtacca aacgcctctt gccaacctct ggcttgattc tgtaaaatca gtctatgcct
     1681 acttatgact ttatctgcaa tacctgtgaa aagcgctttg aagttttttt gacatatagc
     1741 gaatatggaa agaaaacggt tgaatgtacc cattgcaaca gcacagatgt gcgccgccgt
     1801 atgacgaagg tccgcattgc aaaaacggac gaaggcagac ttgaacacat ggctggtgac
     1861 ctaggcagtt tggaagggat ggaaaatgac cctgccgcat ttgctcgtag gatgaaaaag
     1921 atggggaaag agatgggcga agagttgcca gccgagtttg acgaagtggt ggataggctg
     1981 gaagctgggc aaagccccga agaaatcgaa tcagccatgc cagaccttag tgggggcgac
     2041 gagctttcta aggaataaac ctttcccccg acgtataatt tcactaatct tatctaaatt
     2101 gttgtgactt ttgtcacatt aagggatgct tatttttact gtgcattaat ctcccactta
     2161 agtaaaattt ctcatatctg ctaaaaaatg tcaaagcagt cattttctcc aaaggaggca
     2221 tattttcaag aacacgccga tatttaccag cttttttaca ctgatctaag gagaatgaat
     2281 aatgagtctc aaaaaatttc tagttttgct cggggttttg gttgtgctcg gcgcggtttt
     2341 agcggcctgt ggcggcaccc aacccactca agcaggtact gaaacagcca cggaagaagc
     2401 tcccgctgct ccaataccag atactccata cttagctgat tggcaaggct ctgctcacgc
     2461 gaatgtagca gacgaacctt tccgtcactg ggatgacgct gccgaaaatc ctgacggcgt
     2521 tcccacttcc tgcgctaagt gccataccac cgatggttat cgtgatttcc tcggtgccga
     2581 tggttccgaa gccggtaaag tggatgtagc cgttgctgcc gaccatgccc agggcgtgca
     2641 gtgcgtggct tgtcacaacg ctgtaaccat gaccaagacc acggtcatgt tcccctccgg
     2701 tgttgaaatc acagccggtg atgatagccg ttgtatggaa tgtcatcagg gacgtgagtc
     2761 caaggtcagt gtggatgcgc agattgaaaa gtttgccgcc accgaccccg atgccgttgt
     2821 tgctccgatc aaggatgacc agggcaatga tgtgttcttt ggtttccgca atgttcacta
     2881 ctatgctgca gctgccaccc tctacggtgg tatgacccat ggtgggtatg aatatgatgg
     2941 cttgacctac gatgccaaga acacccacac tgatggttat gactcctgca caggctgtca
     3001 tgatcctcat accctcgaag tcaaagttga ccagtgcgct ttctgtcacg aagatgtagc
     3061 caccgtggaa gatctcaaga acatccgcat ggtttcatcc gctcctgact atgatggcga
     3121 tggcaatgtt gaagaaggta tgtattatga gatcgaaggc ttacaagctg ctttgttggc
     3181 tgagatccaa acatatgccg ctgataaggg tggcgctgcg atcatctacg attcagcatc
     3241 ctatccctat tggtttatcg acacaaatgc caatggcgca gccgatgaag gtgaagttgc
     3301 cttcccgaat gcgtattcca cctggactcc tcgtttgctc aaggctgctt ataactatca
     3361 ggtctctatg aaggatcctg gtgcgtttgc ccacggcaac aagtacatcg ttcagttgct
     3421 cttcgactcg atcgccgacc tcggtggcga taccagcaaa ctcgctcgta ccgatgcggg
     3481 tcacttcgct ggcaacaccg aacccttccg ccattgggac aaggatgaag aaggtaatcc
     3541 tgtctatgaa gtagaagccg cttgtgccaa gtgccatagc gctgctggtc tgccgcaatt
     3601 ccttgaatac ggcgccaacc tgcctgtgga atctgccaac ggtttcatgt gctccacctg
     3661 tcacaatgaa gaagcttggc ccgaacgcta cgctgtcgct tctgtgacct tccccagtgg
     3721 ttcaaccgtc agctttggtg gtaaagatgc cgatggcaac ttcgtcgccg acgactccaa
     3781 cctttgtatc gaatgccatc agggtcgctc ttctacaccg actttgaaca aggcactcac
     3841 tggtaaggaa gaagatactc ctgatccaaa gatccgcttc agcaatattc actactttgc
     3901 tgccggcgct accatctttg gtgctgatgt tcagggcgct tacatgtacg aaggcaagga
     3961 atatgtaggt cagaacatgc atgctgacga agctggcaag atgaacaaat gccaggattg
     4021 tcacgatgtt cacgctctcg aacccaaagt cgaagcctgt gaaacctgtc acgacaccac
     4081 cgacccaaca gcagtccgcg aaagcgatgt ggactatgat ggcgacggcg atgtaaccga
     4141 aggtatcaag ggcgagatcg acaccatgac cgaagccctc tatgctgcaa ttcaggcata
     4201 tgctgaagga catggtggcg ccattgaata caaagctact gcttacccgt acttcttcgg
     4261 tgcggatggc aagggatatg cagcgtggac tcctcgcttg ctcaaggctg ccttcaacta
     4321 ccagtacgtc cagaaagacc cgggtgcgta cgttcacaat cccaagttcg ttatccagtt
     4381 cctgtatgac tccattcagg acctgggcgg tgatgtgagc gcgttcacac gcccgtaagc
     4441 tccaagtaat aaaaaatggc ggatggcaac atccgccatt ttctttttaa tccaattctt
     4501 aatcacacca aaaccgtgat atttagcact gtaatggggc gcatgcccca cttacaattg
     4561 gggcaattcc cccacgtttg taatcgggaa tataatatgt aaattgtccg aattttaaaa
     4621 aggagaatgt gatgcaccaa aggatcaaac ttcgcacgcc tcacatctgg accgtgagcc
     4681 tgcttctcgg acttgggctt ttcgccttcg ctctagcagc actcaccaca accgcgcaag
     4741 ccgccccttc tcttcagccc gcagcacaag accaaaaacc gaataacgat ttttgtcttg
     4801 cctgtcacca agaggaaggc atcgacaaaa ccctcggtag cgaatcgctc ccagtaacca
     4861 tcaaccccac tgaatttggt ttatcggttc acgctgaaga aggcgttctc tgtgtagatt
     4921 gccatgtaga tatcagcgac tatccccacc cagacgtaaa atcaaaatcc gttcgtgatt
     4981 ttcaaatgac cttcgccgac tcctgcaaag aatgtcacga agatcagttc accgatgcac
     5041 atgatagtgt ccaccaaacc gcgatggatg aaggcgaccc caacgcccca ctctgcgccg
     5101 actgtcacaa cccccatact cagggacgca tcattggcga aaccagcggc gagctgacca
     5161 ttgccgcccg caaagaagtg cctgccactt gtgccaagtg ccataccgaa actgttgaaa
     5221 tttacaagaa tagtgttcat ggcagcgccc tggcagatgg caatacggat gtgcccacct
     5281 gtacagattg tcacggtgtg cacaacattg gtgaccccac cactgcctct ttccacaaca
     5341 acacacctgc cttgtgcgca gactgtcacg ataacgaaga gttgatgggc aaatatggtc
     5401 tttccaccga agtgcttgaa acctacgtgg cagatttcca cggcaccacc accacactct
     5461 ttgaacagca attccctggt caaccgatca acaccgccgt ctgtacagac tgccatggtg
     5521 ttcacgacat cgctagcacc aagaaccctg aaacggggat tgccatgcag gagaatctgc
     5581 tcgtcaaatg ccagcgctgt caccccgatg caactgctaa tttctcagcc gcatggatgg
     5641 gacactacca acccagccct gagcattacc cgatcgtcta ttacgtcaac ctcttctata
     5701 aattcttcat tcccgcagtg ttgggtggta tggtgctctt tatcctcacc gatatttatc
     5761 gccgcatcat caaccgcgcg aaaggagcaa agcactaatg aatactgtaa ccaaagctta
     5821 cctgcgcttc ccgatcgcgc gccgtattga acactgggtg atgatgctct ctttcacctt
     5881 gctcggtctc actgggcttc ctcagcgctt tccagaggct ggaatttcac aagccattct
     5941 cggtgtcttg ggcggcattc agggtgtgcg cacgattcat cacacgtctg ctattgtcat
     6001 gatgtttggc accgcctggc atattctcgt aatgggttac agcgtcttcg tcttgcgcga
     6061 taagatgtcc atgctgccta cattacaaga tgccaaagat ggtttgcagg cattgctcta
     6121 caatatcggt tttgccaaga cctacccaca aatggggcgc tacaccttcg aagagaagat
     6181 ggaatactgg gcgttcgttt ggggcgcatt ggtcatgggc gccactggtt tcatgatgtg
     6241 gaatccaatt acagcaacaa agttcctgcc cggcgaattt gtaccggctg ccaaagccgc
     6301 tcatggtaac gaagctgtgt tggctgtgct tgctatcatc atctggcaca tgtatggcgt
     6361 gcacatcaaa cgcctgaata aagccatgtt caacggtatg caaaccgaag ccgaaatgct
     6421 gcatgaacat ccgcttgagt tggcagacat caaagctggg attgcagatc gccgccccga
     6481 tgccgcaact ctgcgcaagc gccagatgat ctactatccc atcgccgctg tcttaaccat
     6541 cggcatgctc agtggaatct atggctttat caacgctgaa gaaaccgcgc tgacgaccat
     6601 tccgcccatt gtggagacgg tcgaaccacc tgcaccaacg gttcaaccat aggaacaaga
     6661 atcaaaaaag agccgccctt agaggcggct cttttttgat tctataccga accttaatct
     6721 cccgtaacgg cttcgccaac cttcatggtg ataacttcat attcttcact cacaaggcga
     6781 tgatctacaa cgcgtaagta tttcacaccg acggagaaag ccagcagacc aaaggcgatg
     6841 attcccaacc cggctgccca ttctacgagc gaaggagtgt agctagtgta ctggacgatc
     6901 atctcaccgg gggtatacgg aatgaccgca aggaaaccaa cgatattcgt atcgaagcgg
     6961 aaggctacta cgccgccgac gatcatcgcg aaggcgagca tccgccacag cggagtctga
     7021 cgcagggagc gcttgagcag aataatggta ggaaagagcg cgccgaagag catctcgccg
     7081 aaccagaagt tgaaggagag cggtcctttg gtgaggagtc tgaatccttc ggtgcgtccg
     7141 ggttcgtaag tgtaggtttg ggcaagccaa tcccagaagc gccagtagag gtaagcgatc
     7201 atcatatacc caagcacttg agcgacacgc tcgatcaatg tatcgttgac cttggctttg
     7261 ggcgtaatgc gtgctgccaa catggatgcc aacagtgtga gcgatacgcc gccgaggatg
     7321 gctgtgaaca tgaacatcac agacatttca gggcgatacc aaatggggcg agccttgatg
     7381 acgccatata cagcaccgag tgaagattga tgcaaactgg agagcgccaa gcctaagacg
     7441 gcaagcacag gtgcgtagtg atgcatgtgt tccatctttt cggcgatctt cgggtatttg
     7501 gtgcgtaacc attcaaagga tgcgacgatg ggcaagactt cgaagatcaa taccgtgaag
     7561 tatagaccca cacacatggt tacttcccaa agcagagagt gaggattcca gaagacaaag
     7621 gatttccaga accgttcagg gcgaccaata tccagcaata gcgccatcat tgccatgctg
     7681 tagcccgtga agccaatgaa ggtcgccgtg cgggcgatag gttcatagcg tttgaagccg
     7741 aagaggtaca caattgcgca caaactgaag gcaccagccg ccacggcaat ggacgagaga
     7801 tcgatggtga tccacaaacc ccaagggaca aggttggtga gattggtgat gaccaagcct
     7861 ttccagaaga cggtgagtgc ggcgaccaat gcgctgacca acagaagccc tagaaaagcg
     7921 agccataacc cccaaggggt aaagatattt tcgcgaatgg tttttaggat ggttttcatg
     7981 tcatccctcc acagtggtgt cgtcatttac gggcaggtaa tagacccgcg gtttggttcc
     8041 aagattttcg cgcaagcgat aggacggatg atccttcaag agctggctca cattggaatt
     8101 cgggtcgttc aagtcaccaa agatgcgggc gccggttggg caggcaacta cacaagctgg
     8161 ggtggcggcg gggtcttcac cgggtttaag ccccagtgaa agtccgcggt cgatgcgttg
     8221 atagcagaac gcacatttct ctggcacacc gcgcggacgg cgttccactt ctggttcgcc
     8281 atattgcggc acgtagggat tctcgccatc gaacttttcc caattgaagg agcgtgcatc
     8341 gtatgggcat gccaccatgc aatagcggca accgatgcat ttgtcgtagt ccatcatcac
     8401 aatgccgtca tcacggtaat agctggcacc caccgggcag acttccacac agggagcgtg
     8461 ttcacattgc atacatgggc gcggcagata gacttgcttg ccattcattt cgcccatatc
     8521 cagcaccttg gtccattcca tctcagggtt gatgtcgttg gtggcatggc aagccatatt
     8581 gcattgatta cagcccgtgc acttggattg gtcaatgacc attacccatt gatgctcgcc
     8641 ttttacacct tctgaggctt ttgcttcttc agtgagcaga cgcgttcccg cagcagcggc
     8701 aatgcctact gcagaaagct tgataaagtc acggcggttg aacttatttt gagccatggt
     8761 gtacctcagc agatttcttc aatacgaggc ggtatccgcg ttcgagccag ggagccagca
     8821 acataccggc ggcaatgccg atcagtgctg tgatgcctgc atacccgagc ggagaaacag
     8881 gagcggggtc tggtgaaacg ataggagatt gatctaccgt agcggcggca ggtttgggtg
     8941 tttctgttgg ggcaacgctc gcaacaatag cttgttccgt cgcttcagcc tgaccatctg
     9001 tatgcatttg gttggcatgg caagagttac aggttgtaat gcttgctttg aacgagtggt
     9061 ctgccacttg gtgaac
//
LOCUS       DAOQNJ010000032         9002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3825_length_9002_cov_9.555829, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000032 DAOQNJ010000000
VERSION     DAOQNJ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3825_length_9002_cov_9.555829"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..842)
                     /locus_tag="PLG52_01835"
     CDS             complement(42..842)
                     /locus_tag="PLG52_01835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB40208.1"
                     /translation="MKIDYQEPSKDLLMRIDIHEKYGSANIDVWTNDLLKPQAGMSIL
                     DVGCGAGKLSFLFDDYTKRQAKITGGDFSEELLDKARAKNKERGSNIDFQFLDFNEKF
                     KFEDNTFDLCTSAFAIYYASDLKFTFGEAHRVLKPGGRLFVSGPLPENKQMFYDIIKE
                     ATNATIPPMPGSSRFKGDIFNTIDGIFAKTELHKFENHLTFPEVAPFIDYVRASLSED
                     RRLWTSMFNGKDEYEALIGKITAVAQKWLDRDGKLVMTKVVGGILATK"
     gene            965..2242
                     /gene="hisS"
                     /locus_tag="PLG52_01840"
     CDS             965..2242
                     /gene="hisS"
                     /locus_tag="PLG52_01840"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNB40209.1"
                     /translation="MANKIQAVKGTREFYPEQMALRNFIYSKVRSAAHAFGYQEWDAP
                     FIETIDLYAAKSGEELVKKQSFTFEDRGGDFVTLRPELTPSLARMIAAKQGELTFPVR
                     WWSFGPFWRYEQPQKGRSREFFQWNVDMLGVNSPEADAELIAVGATFLRSVGLSPERA
                     LIYVNNRRLMDSEFDALGISAEKRLDVSNLVDRRTKMEPAKWDAYALDLGLNQTQLEG
                     LKSILGNFELWKKSDELVRLFATLEALGVKEYVKFDPNIMRGLLYYTGTVFEAFDVTG
                     SLKRAIFGGGRYDNLLADVGGQPLSGVGFAMGDVVIGIILQEAGLLPEFIPSPAQVLV
                     TVFDEKLWMQSFALAAELRAAGLNVMVSPEPTKLPKQFKFADKMKMKVALVIGPDEAA
                     QGLVVVKNLVNGEQVQVKKEAVLDSIQSVLKHA"
     gene            2235..2702
                     /locus_tag="PLG52_01845"
     CDS             2235..2702
                     /locus_tag="PLG52_01845"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40210.1"
                     /translation="MPEIRPVTKDNWKELVALKVRDDQKHFVASNLYSIAESQFGETI
                     PGEGHWDMFPYGMYDEGMPVGFLMLSYNYEFARFQGFILRLMVDDAHQGKGYGKFGMN
                     WMLEKYRADERVRVVGISYEPNNEVARTLYASLGFQETGEIVDGEMLALLNLR"
     gene            2733..2807
                     /locus_tag="PLG52_01850"
     tRNA            2733..2807
                     /locus_tag="PLG52_01850"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2765..2767,aa:His,seq:gtg)
     gene            2891..3694
                     /locus_tag="PLG52_01855"
     CDS             2891..3694
                     /locus_tag="PLG52_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNB40211.1"
                     /translation="MKHHLVEPGKKIKLAEVDPQDTGKFKGGKEEGLKEIEKLNEKLQ
                     ELQELLYAEGKHKVLVVLQAMDTGGKDGAIRRVFDGVNPQGVKVASFKVPTSEEMAHD
                     YLWRVHKVVPASGELVVFNRSHYEDVLVVRVHNYVPKEVWSKRYEQINAFEKLLAENG
                     TTILKFFLHISKDEQKERLQARLDDPSKHWKFSVGDLGERKLWDEYQAAYEDALNKTS
                     TEHAPWYVVPANRKWYRDLVISTVLVETLKGLKMKYPEPKDDLSGVVIE"
     gene            3722..5143
                     /locus_tag="PLG52_01860"
     CDS             3722..5143
                     /locus_tag="PLG52_01860"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNB40212.1"
                     /translation="MSRTIHVLFLAAEADPFIKVGGLGDVSGVLPRELRALSDDELKL
                     DVRLVLPYHPAVKAENLRPIEIFSIPRGDSEVTVEAFETTFNGMPVYFIGGEPIHSSG
                     SVYSLDAKLDAEKYAFFSLAAMELPKHINWQPDVIHANDWHTALSLYVNLTKRWEAGA
                     KHIANVLTLHNMPFMGADVRAILEQYRIQLAQTDLPEWARVLPLPLGLWASDAIVAVS
                     PGYAGEVLTEEFGCGLNEFMSLRKETLSGILNGIDTVSFDPETDKSISVNYTLETLDR
                     RVENKELLQEKLGLKRDPNVPLLAIISRMDVQKGVDLAFTALKNMKRIDFQAVILGTG
                     DPKLEEAARELQNLYPDKIKAELRFDAALARQIYAGADMILMPSRYEPCGLAQMIAMR
                     YGCIPVVRSVGGLKDTVIPNETGFIFEKAHHMSLVSAIKAALKIYPDREKWAALQRNG
                     MAKDFSWEASAREYLKLYQSLVK"
     gene            5146..6651
                     /gene="malQ"
                     /locus_tag="PLG52_01865"
     CDS             5146..6651
                     /gene="malQ"
                     /locus_tag="PLG52_01865"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNB40213.1"
                     /translation="MAFKRSSGILLHPTSLPGPYGIGDLGPQAFRFVDWLSSTGCKIW
                     QILPLGPTGYGDSPYQCFSAFAGNPYLISPDDLLAENLVTEEDLVELKELPASKVDFG
                     LFIPKKLELLAKAFQRYQADPEPLREAFNYFCAENAYWLDDFALFMALKEANGGGAWN
                     GWDPALRARKKTALNKAKKEQAENVERFSFYQFLFFRQWTRLRKYANEKGIQIMGDIP
                     IFVAYDSADVWANPDLFFLDEAGNPTVVAGVPPDLFSTTGQLWGNPLYNWKVHKKDGY
                     AWWLSRVRALLQTVDILRFDHFRGFAGYYEIPASHTTAEHGRWVPGPAKDFFRAVDED
                     LGNGVIAPGTGLPIVAEDLGVITPDVIELLNAFDLPGMKVLQFAFTGPENPFLPHNYV
                     PNCVAYTGTHDNNTAKGWYADAPDAEKDFARRYLAVDGHDFSWDLIRAVWRSVAVFAI
                     APMQDVLGLGGEARMNFPSRLGGNWEWRMSESDFREDLAAGLRDLNWMTLR"
     gene            complement(6715..7176)
                     /locus_tag="PLG52_01870"
     CDS             complement(6715..7176)
                     /locus_tag="PLG52_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40214.1"
                     /translation="MLTIDFLEDHLDTIPTLVKWFRNQWTEYYANSSDEAMQQDFLED
                     ILRDRLPSRLVAFEGNELVGTIVLREGDAELSPEHHPELGGLYVAESHRGHGIGTELI
                     KAGMKLAQDQGYSEISATTVKAAGILEGLGWKFIKKIQYPDGEVSLYHFKI"
     gene            complement(7268..7657)
                     /locus_tag="PLG52_01875"
     CDS             complement(7268..7657)
                     /locus_tag="PLG52_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40215.1"
                     /translation="MKTNKAFFTLVTITSIICLCIGCFFAFSMPVYKTLDNPTAEEGQ
                     QKANELIIALEQYKNDTGSYPSELGSLVPDYLSAIPQPTWSAHYSYELQPNEDEFIIS
                     FDVGISFDGDYCEYNSRTKSWHCSDKI"
     gene            complement(7694..8974)
                     /locus_tag="PLG52_01880"
     CDS             complement(7694..8974)
                     /locus_tag="PLG52_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB40216.1"
                     /translation="MRIVIIAPGSRGDVQPYIALGKGLQNAGHYIRLVSHSNFETLVT
                     SYGLEFWSFGNDVKDAVENSEMRELTEKGNFLLLMAKMAKEAEREALRFAEGGLLASQ
                     GMDVVLAGIGGLFIGTAIAEKLHMPLVQAYVFPFTPTKDFSSVLTPKLPTVFNRLSHH
                     LARQLMWQGFRSADGIARKEALNIPSAPFLGPYDSRSTNNMPVLYGFSPSVISTPSDW
                     NGNTHITGYWFIDETNDWQPPVALLDFLKSGPPPLYIGFGSMSNRNPEQTADLVIQAL
                     AMTNQRAILLSGWSGLQKTNVPDSILMVDSIPHSWLFPRVSAVIHHGGASTVAAGLRA
                     GVPSIIIPFFGDQPFWGQRIADLGVGTKPIPRKKLTAEKLANAIREVVTNQEMRQRAE
                     KLGKQIQSENGIKSAVEIFNKLEQQLPNKASKGN"
BASE COUNT         2287 a         1956 c         2243 g         2516 t
ORIGIN      
        1 gaacaatttt tcctcgtcca tgacctatca tttcaggtct gttattttgt cgccagaatg
       61 ccgccaacga ctttggtcat caccaatttg ccgtcacggt cgagccattt ttgagccaca
      121 gcggtaatct tgccaatcaa ggcttcgtat tcatccttgc cattgaacat ggaagtccac
      181 aaacggcggt cttcgctcaa tgaagcgcgg acgtagtcaa tgaacggcgc gacttcgggg
      241 aaggtgaggt ggttttcgaa cttgtgcagt tcggtcttgg cgaagatacc gtcaatggta
      301 ttgaagatat cgcccttgaa gcgggaggag ccgggcatgg gcggaatggt cgcgttggtg
      361 gcttccttga tgatgtcgta gaacatctgc ttgttttcag gcagtggacc ggagacaaaa
      421 agccgtccgc cgggcttaag cacacggtgc gcttcaccaa atgtaaattt caggtctgag
      481 gcgtagtaga tggcaaatgc gctggtgcag aggtcgaagg tattatcttc aaacttgaac
      541 ttttcgttaa agtctagaaa ttgaaaatca atgttcgagc cgcgttcttt atttttggcg
      601 cgcgctttgt cgagcagttc ttcagagaag tcaccgccgg tgatcttcgc ctggcgcttg
      661 gtgtaatcgt cgaacaaaaa gctgagcttt ccggcaccgc aacctacatc gagaatgctc
      721 atgcctgctt gaggtttgag caggtcgttg gtccaaacat cgatgttggc agagccatat
      781 ttttcatgaa tatcaattcg cattaaaagg tctttgctgg gttcctgata gtcgatcttc
      841 atatcatctc tccttgaagt tgatgttggg attataccaa aattcagtgg gtgaattttg
      901 aaatgcttat ttacgattaa tcctggaatg agtcatgtta aaatgcgtgg catttggagt
      961 atacatggca aacaagattc aggcagtaaa aggtacaaga gaattttacc cagagcagat
     1021 ggcgttacga aactttatct acagtaaggt tcgttctgct gcgcatgctt ttggttatca
     1081 ggaatgggat gcgccattta tcgagacgat tgatctgtat gcggcgaaat cgggcgagga
     1141 gttggttaag aagcagtctt tcacgtttga agaccgtggt ggcgattttg tgaccttgcg
     1201 ccccgagctg actccatctt tggcacgtat gattgcggca aaacaagggg agttaacttt
     1261 cccggtgcgt tggtggtcgt tcggtccgtt ttggcggtat gagcagccac aaaaggggcg
     1321 ctcacgtgag ttctttcaat ggaatgtgga tatgttggga gtcaactcac cagaagcgga
     1381 tgcggagcta attgccgtcg gagccacatt cttgcgctcg gtcggactca gcccggagcg
     1441 ggctctgatc tatgtgaata atcgccgcct gatggattct gaattcgacg ctttaggcat
     1501 ttctgccgag aaacgcctcg atgtttctaa tttggtggat cgccgtacaa agatggaacc
     1561 cgccaaatgg gatgcgtacg cccttgacct tggcttgaat caaactcaat tagaaggctt
     1621 gaagtctatt cttggtaatt ttgaactgtg gaagaagagt gatgagttgg tccgtctctt
     1681 tgcgacactg gaagcattgg gcgtcaaaga atatgtcaaa tttgacccga acatcatgcg
     1741 cggacttctc tattacactg gcaccgtctt tgaagccttc gacgtgaccg gttcactcaa
     1801 acgcgccatc tttggcggcg ggcgttacga caatctgctt gccgatgtgg gtgggcaacc
     1861 tctttctggc gtaggctttg ccatgggcga tgtggtcatt ggcattattt tgcaagaggc
     1921 tggtctgctg cctgaattca ttcccagccc ggcacaggtg ttggtaacgg tctttgacga
     1981 gaaattgtgg atgcagtctt ttgcgcttgc ggcagaatta cgtgcggcgg gactcaatgt
     2041 tatggtctcg ccagagccga caaaactgcc caagcagttt aagtttgcag acaagatgaa
     2101 gatgaaagtt gcgctggtta tcggtccaga tgaggcggcg caaggcttgg tggtcgtcaa
     2161 aaatcttgtg aatggcgagc aggttcaggt caagaaagaa gcggttcttg attcgatcca
     2221 aagcgttctg aaacatgcct gagattcgcc ccgtcacaaa agacaattgg aaagagttgg
     2281 tcgccttgaa agttcgggat gaccaaaaac attttgtggc gtcaaatttg tattccattg
     2341 ctgaatctca atttggcgag acgattcctg gcgaggggca ctgggatatg tttccatatg
     2401 ggatgtacga tgaaggaatg ccagttggtt ttttgatgct cagctacaac tatgagtttg
     2461 cgagatttca gggttttatc ctgcggttga tggtggacga tgcgcatcag ggcaaggggt
     2521 atggcaaatt cggtatgaac tggatgctcg aaaaatatcg cgcggacgag cgtgtgcggg
     2581 ttgtggggat tagttatgag ccgaataatg aagtggcgcg tacattatat gcaagcttag
     2641 ggtttcaaga gactggcgag atcgtagatg gcgagatgtt ggcactcttg aacctgcgct
     2701 gaattgtggt aaaattttgt ccgctttata tggcggatgt agctcaatgg cagagcatca
     2761 cactgtggct gtgagggttg tgggttcgaa acccatcatc cgccccaaaa agacctggtc
     2821 cttaatctag gtctttttgt tttaatatga tatatacctg tgcaataaag gttttaatat
     2881 cggaggtctc atgaaacatc atttggttga accgggtaaa aagatcaaat tagcagaggt
     2941 agacccgcag gatacgggga agtttaaggg cggtaaggaa gaaggactta aagaaattga
     3001 gaaattaaat gaaaaactgc aagaattgca ggagttactt tatgctgaag ggaagcataa
     3061 ggtcttggtg gtgttacagg caatggacac cggtgggaaa gatggtgcca ttcggcgggt
     3121 atttgacggc gtcaacccgc agggggtgaa ggtggcaagc tttaaagtgc cgacctcaga
     3181 agaaatggcg catgattatt tatggcgtgt gcataaagta gtgcctgcca gtggagaatt
     3241 ggtggtgttc aaccgcagcc attacgaaga tgttttagtg gtgcgtgtac ataactacgt
     3301 gccaaaggaa gtttggtcga agcgttatga gcagatcaac gcttttgaga agcttctggc
     3361 agaaaacggc acgactatcc tgaagttctt ccttcatatt agtaaagatg agcagaaaga
     3421 acgcctgcaa gcacgcttag atgacccatc caagcattgg aagttcagcg ttggtgacct
     3481 tggcgagcgc aaattgtggg acgagtatca ggcggcttat gaagatgcat tgaataagac
     3541 cagcaccgaa catgccccgt ggtatgtggt gcctgccaac cgcaaatggt atcgcgattt
     3601 ggtgatttcc acagtgctgg tggagacgct caaaggctta aaaatgaagt accccgagcc
     3661 gaaagatgat ttgagcgggg ttgtgatcga gtaagtctgt aaagggtgta aaatagcgct
     3721 catgtctaga acaatccatg tgctttttct cgctgccgaa gccgaccctt ttatcaaagt
     3781 tggcgggctg ggcgatgttt caggggtgct gccgcgagag ttgcgcgctc tttcagatga
     3841 cgaattgaaa ttagatgtga ggttggtttt gccgtatcac ccagcggtga aagcggaaaa
     3901 tttgcgccct atcgaaattt tttccattcc gcgcggggat tctgaagtta ctgtcgaagc
     3961 gtttgaaact acatttaatg gaatgccggt ctattttatt ggcggtgaac cgatccattc
     4021 cagcggctcg gtgtattcct tagacgcaaa attggatgct gagaagtatg ctttcttctc
     4081 actggcggcg atggaactcc ccaagcacat caactggcaa ccagatgtta ttcatgccaa
     4141 cgactggcat accgcactga gtttatatgt caatttgacc aaacgctggg aagccggtgc
     4201 aaaacatatt gccaatgtac ttaccctgca taacatgcct ttcatggggg cggatgtccg
     4261 tgctattctt gagcagtatc gcattcaatt ggctcaaacc gatttgcccg aatgggcacg
     4321 tgttttgccc ctacccttag ggctttgggc gtcggatgcg attgtcgctg tctcaccggg
     4381 gtatgcgggg gaagtgctca cagaagaatt cggttgtggc ttgaacgaat tcatgtcttt
     4441 gcgcaaagaa accttgagcg gtattttaaa tggcattgat acagtttctt ttgacccaga
     4501 aaccgataaa tccattagtg tcaattacac cctcgaaaca ctcgaccgcc gagttgagaa
     4561 taaagaactt ctgcaagaga aactagggct caagcgtgac ccgaatgttc cgttgcttgc
     4621 tattatttca cgtatggatg ttcaaaaagg cgtagacctt gccttcactg cgctaaagaa
     4681 tatgaagcgc atcgacttcc aagctgtgat tcttggaacc ggcgacccga agttggaaga
     4741 agcggctcga gagttgcaaa atctttaccc cgataagatc aaagccgagt taagattcga
     4801 tgcggcactt gctcgtcaaa tctacgcagg cgccgatatg atcctcatgc cgtctcgcta
     4861 cgaaccgtgt ggtctcgcac aaatgatcgc catgcgctat gggtgtattc cggtcgtgcg
     4921 atctgtgggc gggctaaaag atacggtcat cccaaatgaa acgggcttca tctttgaaaa
     4981 ggcacatcat atgtcattgg tcagtgccat caaagccgca ttgaaaattt atccagaccg
     5041 tgaaaagtgg gcagccttgc agcgcaatgg catggcaaaa gatttttcat gggaagcttc
     5101 agcacgcgaa tacctaaaac tctatcaatc gttggtgaaa taaacatggc attcaaacgt
     5161 tcttccggta ttcttcttca tcctaccagt ttgcccggtc catatggcat cggcgacctt
     5221 ggtccacaag catttcgttt tgtcgattgg ctttcatcta caggttgcaa gatctggcaa
     5281 attttgccat tgggtcccac aggctatggc gattcgccgt atcaatgttt ttctgccttt
     5341 gcaggaaacc cttacctcat tagcccagat gatttacttg ccgaaaactt ggtgactgaa
     5401 gaagaccttg ttgaacttaa agaacttccc gcctcaaaag tggactttgg cttgttcatc
     5461 cctaaaaagc ttgagctttt ggcgaaagcg tttcagcgct accaagccga ccccgaacct
     5521 ttgcgtgaag ccttcaacta tttctgcgcc gaaaacgcct actggctcga cgactttgcc
     5581 ctcttcatgg cattgaaaga agctaacggt ggtggcgctt ggaacggttg ggaccccgcc
     5641 ttgcgtgctc gtaaaaagac ggctttgaat aaagcgaaaa aagaacaagc tgaaaatgta
     5701 gagcgttttt ctttctatca attccttttc ttccgccagt ggacccgcct acgcaagtac
     5761 gcgaatgaaa aggggattca gattatgggc gacattccca tcttcgtcgc ctacgactcg
     5821 gccgatgtat gggcaaaccc ggatcttttc ttccttgatg aggcgggtaa tcccactgtg
     5881 gtggcagggg ttccacctga tcttttctca acgactggtc aattgtgggg taacccgctt
     5941 tataactgga aggttcacaa aaaagatggt tatgcttggt ggctttccag agtccgcgct
     6001 ttgttgcaaa cggtagatat tctgcgtttt gatcacttcc gcggttttgc gggatattac
     6061 gaaattccag cgtctcacac cactgccgaa catgggcgtt gggtgccggg tcctgccaaa
     6121 gactttttcc gcgcagtgga tgaggatctt gggaatggcg tgattgcccc aggaacaggt
     6181 ttgccaattg ttgccgaaga tttgggcgta atcactccag atgtgatcga attgttgaac
     6241 gcttttgatt tgcctggcat gaaagttttg caattcgctt ttacgggtcc agagaatccc
     6301 ttcttgccgc ataactacgt cccgaattgt gttgcttaca cgggcactca cgataacaat
     6361 accgccaaag gttggtacgc tgatgccccc gacgcagaaa aagattttgc tcgccgctac
     6421 cttgccgtgg atggacacga cttctcgtgg gatttgatcc gcgctgtgtg gcggtctgtg
     6481 gcagtctttg ccattgctcc catgcaagat gtgcttggct tgggcggaga agcccgcatg
     6541 aactttccaa gccgcctcgg tggtaactgg gagtggcgca tgtctgaaag cgacttccgt
     6601 gaagaccttg ccgcaggtct gcgagacctc aactggatga ccttgagata aaaagaaacc
     6661 gcctcgtatg aggcggtttc tttttattga ggtgagatgc ttttttgaat tatcttagat
     6721 tttaaaatga tacaatgaaa cttccccgtc tggatattgt attttcttga tgaacttcca
     6781 acctaaaccc tctaagatgc ctgctgcttt aaccgttgtt gcagaaattt ctgaataacc
     6841 ctggtcttga gctaatttca tacctgcttt aatcaattct gtaccaatgc cgtgaccgcg
     6901 atgagactct gcaacataca aacctcctaa ttcaggatga tgttcaggcg ataattcagc
     6961 atcgccttct cgcagtacaa tcgtaccaac aagttcattg ccttcaaatg caacgagtcg
     7021 agagggtaag cggtctctca aaatatcttc gagaaaatct tgctgcatgg cttcgtctga
     7081 cgaattggca taatactctg tccattggtt acgaaaccac tttaccaatg taggtatagt
     7141 atcaagatga tcttcaagga agtcaattgt aagcatattt ttttctgctt tcttaaagca
     7201 gatacttttc ggttttaggg tttgagaaac gatcttgtat atgctctgat tttactggtt
     7261 agaactatca aattttatcg ctacaatgcc agctttttgt ccgactatta tattcgcaat
     7321 aatccccgtc gaaacttata cccacatcaa aggagataat gaactcatcc tcattgggtt
     7381 gaagttcata agaataatgg gcgctccatg taggctgtgg tatggcagat aaataatctg
     7441 gaacaagtga acctaattct gagggataac ttcctgtatc atttttgtat tgttccaagg
     7501 ctattatcaa ctcgttagcc ttttgttgcc cttcttcggc tgtagggtta tcgagtgttt
     7561 tatacactgg catcgaaaag gcaaaaaaac atccaataca taaacatata atagatgtaa
     7621 ttgttacaag agtgaaaaaa gctttatttg tcttcattag gtttgcaata tgtcatcctg
     7681 ctgggacgtt atcctaattc cctttggatg ctttgttcgg caattgctgt tcaagtttat
     7741 taaatatttc tacagcagat tttattccat tctcggattg aatttgtttt cctaactttt
     7801 cagcacgttg acgcatttct tgattagtca ctacttctcg aatagcattt gcgagctttt
     7861 cagccgtaag tttctttcgc ggaatcggtt ttgtccctac tcctaaatca gcaatccgtt
     7921 gaccccaaaa tggttggtct ccaaaaaagg gaataataat ggatgggact ccagccctga
     7981 gacccgcagc cactgtgctc gccccgccat gatgaataac agcggacaca cgcggaaaca
     8041 gccatgaatg cggaatagag tccaccatta atatcgagtc tggcacattt gtcttttgta
     8101 gaccgctcca acctgaaagc aagattgctc gttgatttgt cattgctaat gcttggataa
     8161 ctaaatcggc tgtttgttca gggttgcgat tgctcatact gccaaaccca atatacagtg
     8221 gcggaggacc cgatttcaag aaatccagca gagcaacagg cggttgccaa tcatttgttt
     8281 catctataaa ccaatagcca gtaatgtgag tattgccatt ccaatctgat ggtgtagaaa
     8341 ttactgaagg actaaaacca taaagaacag gcatgttatt tgtcgatctt gaatcgtaag
     8401 gtcctagaaa tggagccgat ggtatgttca acgcttcttt tcttgctatg ccatctgctg
     8461 accgaaaccc ctgccacatt agttgacggg caagatgatg tgacaaacga ttgaatactg
     8521 ttggcagctt tggtgtaaga acactggaaa aatcttttgt gggtgtgaaa ggaaaaacgt
     8581 aggcttgaac gagcggcata tgtagttttt ctgcaattgc agtgccaatg aataaccctc
     8641 ctatgcctgc caacacaaca tccattcctt gtgaggctag taaaccacct tcggcaaaac
     8701 ggagtgcttc acgctcggct tcttttgcca tttttgccat tagcaaaaga aaatttccct
     8761 tctcggtcaa ttctcgcatt tcactatttt caacggcatc ttttacatca tttccaaaag
     8821 accaaaattc aagcccgtat gatgtaacca atgtctcgaa attgctatgg cttacaagac
     8881 gaatgtaatg tcctgcattc tgcaagcctt ttcctaatgc aatataaggt tgaacatccc
     8941 cgcgacttcc tggtgcaatg attacgatac gcatcgtgta tgaattatcc tcttatcaag
     9001 aa
//
LOCUS       DAOQNJ010000033         8931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3860_length_8931_cov_8.061965, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000033 DAOQNJ010000000
VERSION     DAOQNJ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8931
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3860_length_8931_cov_8.061965"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLG52_01885"
     CDS             <1..399
                     /locus_tag="PLG52_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tungsten ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB40217.1"
                     /translation="KEVSFWTKAELDPAGQAWFFETGQGMGASLTVASEKQAYILTDR
                     ATYLANKDNLQLEILLEGNNALLNVYHVITVNPTKSDKINYEGALAFANFMVAPETQA
                     VIGEFGIEKFGQPLFIPDAGKDPKELGLDS"
     gene            456..1100
                     /locus_tag="PLG52_01890"
     CDS             456..1100
                     /locus_tag="PLG52_01890"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40218.1"
                     /translation="MSEILEITTLSLQVSMLATIISLVIGLPFGTWLALGNFHGRSVV
                     LSIVNTGMALPPVVVGLVVAMMLWRSGPLGDLRLIYTPWAIVIAQTVIATPMVAGLTT
                     AALEALDPRLKQQLLGLGASQLQMVWHLWREARLPLLAALMAGFGSVISEVGASMMVG
                     GNIRGQTRVLTTAIVLETSKGEFERALALSALLLVITYLINLGLTWIQQKGKKR"
     gene            1125..1844
                     /locus_tag="PLG52_01895"
     CDS             1125..1844
                     /locus_tag="PLG52_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40219.1"
                     /translation="MANIIEISNLIVKRNGRETLRIDSLHIPKGETLAVVGPNGSGKS
                     TFLLTLAHLLKAASGDILLHGKHLKEWNELEYRRQIAFVFQDPLLMDMSVRENIRLGL
                     KFRKMDKKETDARVDKWATAMGVEKLLERRANQLSGGEAQRVSLARAFVLDPELLLMD
                     EPFSAVDPQTREKLLEDLSRVLAENQRTTIFVTHNLKEAAQFGDRVAVMIGGEMKQVG
                     APAQIKESPVDASVKDFLKGL"
     gene            1909..3177
                     /locus_tag="PLG52_01900"
     CDS             1909..3177
                     /locus_tag="PLG52_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNB40220.1"
                     /translation="MPEFLQLLPPEEARKLLFSNLPHAKIKQENIETASSLGRVIAED
                     ISAPHSLPEFLRSTVDGYAVRARDTFGASDSMPAYLSLVDEIPMGSAPSQNIGSGQCG
                     LIHTGGMLPPGADAVVMLENTQVALGGNRPTPGSEIEIFRAVADGENVIQIGEDVKSG
                     SVILKGGTRIRPAEVGGLMALGITHLSVAAKPKVGLISSGDEIIDPFKQTQPGQVRDI
                     NAYTLASLVTKTGGEPILYGIVPDVRADLESTTRRALNECDVVVITAGSSASTRDMTA
                     DVIASLGAPGVLVHGINTRPGKPTILGVCNGKAVIGLPGNPVSALVNGYLFVVPVIER
                     LLGETPRPRPIIAAKLTVNLPSQAGREDWIPVKLIAQSGNAGSQTFQAEPIFGKSNLI
                     FTLTAADGLLRIPPDVTGYGAGEMAEVVLF"
     gene            3213..5174
                     /locus_tag="PLG52_01905"
     CDS             3213..5174
                     /locus_tag="PLG52_01905"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HNB40221.1"
                     /translation="MSIYLHDIPLSTAQNHFRTSLEEVGLWRVLGSETIPLDENALRR
                     VLSEPIWASISSPHYHASAMDGFAVVAENTSGAGPSTPLTFPIEAESAHYVDTGDPLP
                     EWANAVIPIENVESLDENNAITSNIQRPASIRIRAAVSPWSHIRPLGEDIVATQLVLP
                     SGHTLRPADLGAIAASGHQSIQVARKPKVAILPTGTELVPIGSKLKSGDILEYNSLVL
                     AAQIKTMGGDPTRYPITKDDFDLICNNVSEAAQDHDLILLNAGSSAGMEDFSSKVVEK
                     LGQLLVHGVAVRPGHPVILGIIDRDGKKVPIVGVPGYPVSAALTTDIFVEPLIAKWLG
                     RRPLELPTEEAKLTRKITSPAGDDDFVRVAAGMVGGNLLAAPLSRGAGVITSLVQADG
                     LVLLPSGVQGMEAGGKVQVRLYRTKSELERTIFCIGSHDLILDLMAQFLTESDRRLVS
                     ANAGSQGGLISVKRGEAHMAGTHLLDPETGEYNISYIRRFVPDVPVKLVTLAGREQGL
                     VVRRGNPKAVHDLKDLTRADVQFINRQRGSGTRVLLDYHLNQLGLSAEHINGYYQEEY
                     THLGVAAAVASGRATCGLVIAAAAQALDLDFIPLFQERYDLVFPQEYANSPLLAPLFD
                     LLAAPRFRTAVSKLAGYDTTQTGNIVLND"
     gene            5277..>5986
                     /locus_tag="PLG52_01910"
     CDS             5277..>5986
                     /locus_tag="PLG52_01910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB40222.1"
                     /translation="MTILSTKSTTAWAEVDENGRLILPPEVAQRYGLHPGSKVRLDEG
                     NNIVRMHRPVTHLTKIYIEPTVACNLDCITCFRNAWDQPIGKMTDETFESIFNSLKQM
                     DPIPSVYFGGIGEPLFHPKTIEWIRRIKQELGVKVELITNGTTLTERKTRELIDAGLD
                     ILWVSLDGATPESFSDVRMGAELPVILENLRRLFRMRNGGHFPQPEIGVAFVAMKRNI
                     NDLPKVIKLGHTFGARYFS"
     assembly_gap    5987..6086
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6087..6392
                     /locus_tag="PLG52_01915"
     CDS             <6087..6392
                     /locus_tag="PLG52_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNB40223.1"
                     /translation="WTGEVSPCWPLMHTHMSYLHGKPRLSKKHVIGDTRKQSLEEIWL
                     SPEYVAYRERLHNFVFAPCTFCGGCDLSETNEEDCLGNDIAPVCGGCLWAQGIIQCP"
     gene            6471..6803
                     /locus_tag="PLG52_01920"
     CDS             6471..6803
                     /locus_tag="PLG52_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNB40224.1"
                     /translation="MQLRFYANMRTTVGMPLLEINEAMVDSFRKLIPLLLEQYPETAF
                     HLLDEHGELRPDVPVYVDGRNPRLSSVGMDTPLKPDSVISFFSPISSGKLNVEVLREP
                     TIDTRSES"
     gene            6800..7078
                     /locus_tag="PLG52_01925"
     CDS             6800..7078
                     /locus_tag="PLG52_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNB40225.1"
                     /translation="MKVNFFATLRDIAGGKTVEFDLDHGITARELLDAICERFPAMKK
                     ELLKEDGEMHGHVHFFINGRDVQFTDLQFATPITQGDTVNVFPAVGGG"
     gene            7220..8224
                     /gene="moaA"
                     /locus_tag="PLG52_01930"
     CDS             7220..8224
                     /gene="moaA"
                     /locus_tag="PLG52_01930"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNB40226.1"
                     /translation="MTILDTLNRPLRDLRISVTDRCNFRCVYCMPKEIFGSDYKFLHH
                     SQVLTFEEITRIARLFAGLGVKKIRLTGGEPLVRKDFPDLVALLAQIPDLDLTMTTNG
                     SLLPRFAEQLKKSGLNRVTVSLDSLDNKIFKSMNDVDFPVEKVLEGMDAAKAAGLEPI
                     KVNMVVKRGVNEDSILPMARFFREKGFILRFIEYMDVGSTNGWRMNDVVSAKEIISII
                     NAEMPLEPADPNYRGEVAERWRYKDGQGEIGVIASVTQAFCRDCNRARLSAEGQLYTC
                     LFAAKGHDFRDMLRNGASDEEILHKIETVWGKRTDRYSEIRSDNTIPLPKVEMSHIGG
                     "
     gene            complement(8305..>8931)
                     /locus_tag="PLG52_01935"
     CDS             complement(8305..>8931)
                     /locus_tag="PLG52_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNB40227.1"
                     /translation="GNILADGANAVAAHFGHPELAMTVKGQGVPAYDPRGIKGMGLGY
                     ATSNRGACHLRGYTPAAEVVGNVLGPSTVADPLAWKGKGEMNFIFQNVHAVTDCLDIC
                     KFATFAESLDSFAMQYEAMTGVPCDAGHLLKVGERVYNLERHINNLNGFREGSDTLPK
                     RFTDEPSTYGGSKGQVCELDDMLVEYYEKRGWVNGVVPESKLKELEII"
BASE COUNT         2110 a         2283 c         2281 g         2157 t
ORIGIN      
        1 aaagaagtga gcttctggac gaaagccgag ctcgacccgg cgggtcaggc ttggttcttt
       61 gaaaccggtc agggcatggg cgcttcactc accgttgctt cggaaaagca agcctatatt
      121 cttaccgacc gcgctaccta cctcgccaac aaagacaacc tccagttgga gattctgctc
      181 gaaggcaaca atgcgttatt gaatgtttat cacgtcatca ccgtcaaccc gacaaagtcg
      241 gacaagatca actacgaagg tgctttggcg ttcgccaact tcatggttgc ccctgaaact
      301 caggcagtga tcggcgaatt cggcatagag aagttcgggc aaccgctctt catccccgat
      361 gctggcaaag accccaagga acttggtctc gatagttaaa gttgaagtat catctggacg
      421 gctctcacga gccgtccatt tcttttaaat caaccatgag cgaaatcctc gagatcacaa
      481 ccctttcttt gcaagtctcc atgctggcaa caatcattag ccttgtgatt ggtttgccct
      541 ttggaacatg gctggcactt ggcaattttc acgggcgctc tgttgtgctg agcattgtca
      601 acacggggat ggcattgccg cctgtggtgg ttgggctcgt ggtggcgatg atgctatggc
      661 gcagcggtcc gcttggtgat ctacgtctca tctacacgcc gtgggcaatt gtcatcgcac
      721 agaccgtgat tgccaccccg atggtggcag gcttgaccac agccgcactc gaagcgctcg
      781 acccaagatt gaaacaacaa cttctgggac tcggcgcctc acaactccag atggtttggc
      841 acctgtggcg ggaggctcgt ctgccgctgt tggcggcgtt gatggcaggc ttcggctcgg
      901 tcatctcgga ggtgggcgca tccatgatgg tgggcggaaa catccgcggg cagacgcgag
      961 tgctaaccac ggcgatcgtg ttggaaacat ccaaaggcga attcgagcgg gcgctggcac
     1021 tctcggcatt gttactggtg attacttacc tcatcaacct ggggttgaca tggattcaac
     1081 agaaaggcaa aaaacgatag tccagtcttt cgttgaaaaa caccatggca aatatcattg
     1141 aaattagcaa tttgatcgtt aagcgcaacg gacgcgagac attacgaatc gattcgcttc
     1201 atattcccaa aggcgaaaca ttggctgtag taggtccgaa tggttcaggg aaaagtacgt
     1261 ttctacttac gctggcgcat ttgcttaagg cagcaagtgg agacattctt ttgcatggaa
     1321 aacatctcaa agaatggaac gaactcgaat atcgacggca gattgccttt gtctttcaag
     1381 acccgctact gatggatatg tctgtacggg aaaacattcg gctggggttg aagttccgca
     1441 agatggataa aaaagaaacc gatgcgcggg ttgataagtg ggcaactgcc atgggcgtgg
     1501 aaaaacttct ggagcgcaga gccaaccaac tctctggggg tgaggcgcag cgagtcagct
     1561 tggcacgggc gtttgtcctt gatcccgaat tgcttttgat ggacgaaccg ttttcagcag
     1621 tggacccgca gacccgtgaa aaattacttg aagacctttc gcgtgtgttg gcagagaatc
     1681 agcgcaccac tatttttgtg actcacaatt tgaaagaagc ggctcagttt ggcgaccgtg
     1741 tggcagtgat gattggcggc gagatgaagc aagtcggtgc gccagcgcag atcaaagaaa
     1801 gccctgtgga cgcaagtgtg aaggattttt tgaaggggtt ataggacgtt gggtagtatt
     1861 gtataatcga gttgctttat gacattctga tgtgatgagc tgactccaat gcctgaattt
     1921 ctgcaattac taccacctga agaagcacgc aagttattat tctccaacct ccctcatgca
     1981 aagatcaaac aagaaaatat agagaccgcc tcatcgcttg ggcgagtgat cgctgaagat
     2041 atttctgcgc cgcattcact gcccgagttt ttgcgctcga ctgtggatgg ctatgctgtg
     2101 cgcgcacgtg atacctttgg cgcttctgac tcgatgccag catacttgag cttggtggat
     2161 gaaatcccga tgggcagtgc tccatcgcaa aacattggct ctggtcaatg cgggctgatt
     2221 cataccggcg gaatgttgcc gcccggagca gatgcagttg tgatgttgga aaatacacag
     2281 gttgcactgg gtgggaatcg cccaacaccg ggcagtgaaa tagaaatctt tcgcgctgtt
     2341 gccgatggtg aaaatgtaat tcagatcggc gaagatgtga aatcaggctc tgttattttg
     2401 aaaggcggca ctcgcatccg tcctgcagaa gtaggcggac tgatggcgtt gggcatcaca
     2461 catctttcgg tggcagcgaa acctaaagtg gggttgatct cttcaggcga tgaaatcatt
     2521 gacccattca aacaaacgca accagggcag gttcgagata tcaacgccta tacgctcgca
     2581 tcgttggtca ccaagaccgg cggtgaaccc attctgtatg gcatcgttcc agatgttcgc
     2641 gctgacttgg aatccacaac ccgccgcgcc ttgaatgaat gtgacgtggt ggtcatcact
     2701 gctggttcct ctgccagcac ccgcgacatg accgccgatg taattgcttc attgggcgcg
     2761 ccaggcgtgt tggttcatgg aatcaatacc cgccccggca agccgaccat ccttggagta
     2821 tgcaatggta aagccgtgat tgggctgcca ggcaaccccg tcagtgcttt ggtgaatgga
     2881 tatttattcg tggtgcctgt gattgaaaga ttgctcggtg aaacgcctcg tccccgcccg
     2941 atcattgctg ccaaactgac ggtcaacctg ccctcgcaag ctgggcgcga agattggatt
     3001 cccgtcaaac tgattgcaca atcggggaac gctggcagcc agacctttca agcggaacct
     3061 atctttggta aaagcaattt gatctttact ctaaccgccg ccgatggtct cttgcgcatt
     3121 cctcctgatg tcactggata tggcgccgga gagatggctg aagtcgtttt gttctaaaat
     3181 tccagcatcc tttgtaagca tggagacaaa tcatgtctat ctaccttcat gatattccgc
     3241 tgtctacagc gcaaaatcat ttccgaacat ctttggagga agtcggctta tggcgggtgc
     3301 ttgggtcaga aacaattcca ttggatgaaa acgcactcag gcgcgtcctt tcggaaccga
     3361 tctgggcatc catctcctca ccacattatc acgcctccgc catggacggg tttgcggtcg
     3421 ttgccgaaaa cacctctggt gcgggtccct ccaccccgtt gaccttcccc atcgaagccg
     3481 agtccgctca ttatgtagat acaggcgacc cgttgccaga atgggcgaac gccgtcatcc
     3541 cgattgaaaa tgttgaatcg ttggatgaaa acaatgccat cacctcaaat atccaaagac
     3601 ccgcatccat tcgcattcga gcagcggttt cgccatggag tcacatccgc ccgttgggag
     3661 aggatattgt cgccacgcaa ttggttttgc catcgggtca tactctacgc cccgcagacc
     3721 tgggtgccat tgcagcatcg gggcatcaaa gtattcaagt ggcacgcaaa cctaaagttg
     3781 ccattctgcc aacggggact gaattggttc caattggctc gaaattaaaa tcaggtgaca
     3841 ttttagaata caactcactg gttttggctg cacagatcaa aaccatgggc ggtgacccaa
     3901 cgcgctaccc gatcacaaaa gatgattttg acctgatctg caacaacgtc tcagaagctg
     3961 cacaagacca cgacttgatt ttgctcaacg ctggctcctc cgcaggcatg gaagattttt
     4021 cctccaaggt ggttgaaaag ctggggcaac ttttggtaca tggtgttgct gtgcgccccg
     4081 ggcatcctgt gattttgggc atcatcgatc gggatgggaa aaaagttccc attgtaggcg
     4141 tgccgggtta tcccgtctct gctgcgctca ccaccgatat ttttgttgaa ccgctcatcg
     4201 caaaatggtt gggacggcgt ccgctggaat tgccaaccga agaagccaaa ctgactcgca
     4261 agatcacctc ccctgctggc gatgatgatt tcgtccgcgt ggcggcgggc atggtgggcg
     4321 gcaatttatt agcagcccca ctctcgcgtg gcgcgggcgt catcacttca ctggtgcagg
     4381 cagatggttt ggtccttttg ccatcgggtg tgcaaggcat ggaagcaggc ggcaaggtac
     4441 aggtccgctt gtaccgcacc aaaagcgaac tcgaacgcac catcttttgc atcggctcgc
     4501 atgatttgat cctcgacctg atggcacaat tcctgaccga atcagatcgc agattagtct
     4561 ctgccaatgc tggttcacaa ggcgggctga tctcggtcaa gcgcggtgag gcgcacatgg
     4621 cgggcacaca tctgcttgac cccgaaactg gtgagtacaa catctcctat attcggcgct
     4681 tcgtgcccga tgtgcccgtc aaacttgtca cactcgctgg gcgtgaacaa ggtctggtag
     4741 tgcgccgtgg gaatcccaaa gcggttcacg acctcaaaga cctcacccgc gccgacgttc
     4801 aattcatcaa ccgccaacgt ggttcaggca cgcgcgtgtt gctcgattat cacctcaatc
     4861 aacttggctt gtccgccgaa catatcaacg gatactatca agaagaatac actcacctcg
     4921 gcgttgccgc agcagttgcc tcgggtcgcg ccacctgtgg cttggtcatt gccgctgccg
     4981 cccaagccct cgatctggat ttcatcccgc tctttcagga acgctatgat ctagtcttcc
     5041 cgcaggagta tgccaactcg ccgctcctcg cgcctctctt tgacctgcta gctgctcccc
     5101 gcttccgaac cgcagtttcc aaactggctg gctacgacac gacccaaact ggcaacatcg
     5161 ttctaaacga ctgacatacg ttccgctcct ccacaaaaaa tgtaactttc gtcacatgtc
     5221 ttttccgtca aaacggatat gatatgtaaa cattgttttg ttagtaagga gaacgtatga
     5281 ccatcctctc gacaaaatcc accaccgcct gggctgaagt agacgaaaac ggacgcctca
     5341 ttcttccgcc cgaagttgcc cagcgttacg ggctgcatcc tggctcgaaa gttcgcctcg
     5401 acgaaggcaa caacatcgtc cgcatgcatc gcccggtgac tcacctcacc aaaatttaca
     5461 tcgaacccac cgtcgcttgt aacctcgact gcatcacctg cttccgcaat gcctgggatc
     5521 aacccatcgg caaaatgacc gatgaaacct ttgagagcat cttcaacagc ctcaaacaaa
     5581 tggacccgat tcccagcgtc tattttggcg gcattggcga accgctcttt catcccaaaa
     5641 ccatcgaatg gatccgccgc attaagcagg aactcggcgt aaaagtagaa ctcatcacca
     5701 acggcaccac cctcaccgaa cgcaagaccc gtgagttgat cgatgccggt ctggatattc
     5761 tctgggtgtc tctagatggc gccacccctg aaagcttctc agatgtacgc atgggcgctg
     5821 aactccccgt cattctagaa aacctgcgcc gactttttag aatgcgtaat ggcgggcact
     5881 tcccacagcc agaaattggc gtcgcctttg tggcgatgaa gcgtaacatc aatgacctgc
     5941 ccaaggtcat aaaacttggg cataccttcg gtgcccgtta cttctcnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnntgga cgggggaagt cagcccgtgc tggcccttga
     6121 tgcacacgca catgagctat ttgcatggca aaccgcgcct ttcaaagaaa catgttatcg
     6181 gcgatactcg caaacaatca ctggaagaga tctggctcag cccagaatat gtcgcatatc
     6241 gcgaacgcct gcacaacttt gtattcgcac cctgtacctt ttgcggcggc tgtgacctct
     6301 ctgaaaccaa cgaagaggat tgcctcggca atgatatcgc cccggtctgc ggcgggtgcc
     6361 tctgggcgca gggcattatt caatgcccat aatttgatct agggttttca aagagctcgg
     6421 gtcttccgag ttctttgttt taacacgaac gaaaatgata aaatcagaac atgcagctac
     6481 ggttttatgc caatatgcga accacagttg gaatgccatt gctcgaaatc aacgaagcga
     6541 tggtcgattc atttcgcaag ttgataccgc tactcctcga acagtatccc gaaactgcat
     6601 tccatttact ggatgaacat ggcgaattac gcccggatgt tcccgtctat gtggatggaa
     6661 gaaacccgcg tctttcctcg gttggaatgg atacccctct caaaccggat tctgtcatca
     6721 gctttttctc ccccatctca agcggaaaac taaatgtaga agtgttacgt gagccgacta
     6781 ttgacacaag gagtgaatca tgaaagtgaa tttctttgcg accttacgtg atattgctgg
     6841 cggtaaaact gttgagttcg accttgacca tggcattact gcccgtgaat tgttggatgc
     6901 catttgtgaa cgcttcccag caatgaaaaa ggaattactc aaggaagatg gcgagatgca
     6961 tgggcatgtc cacttcttta ttaatgggcg ggatgtacaa tttactgacc tgcaatttgc
     7021 cacgccgatc acacaaggcg atactgtgaa tgtcttcccc gcagtgggcg gcggttaatt
     7081 caggcaaatt tcgaaattga aaaagcttag aaacaccgta tcaaacctga gattaagaaa
     7141 gatgcggttt ttaggtcaaa aacaacatat ccttacgggt ataatccttt atgtcagagc
     7201 ttgttgacac tcacctatta tgacaattct agataccctc aaccgtcctc ttcgtgactt
     7261 gcgcatctcg gtcactgatc gctgtaattt tcgctgtgta tactgcatgc cgaaggaaat
     7321 ctttggctca gattataagt ttctgcatca cagccaggtg ctgactttcg aagagattac
     7381 gcgcattgca cgtttgtttg ctggcttggg ggtcaagaag attcgcctta ctgggggcga
     7441 accccttgta cgtaaagatt ttcctgacct tgtggcatta ctggcacaga tccccgacct
     7501 tgatctcacg atgacaacca acgggtcgct tctgcctcgg tttgccgagc aactcaaaaa
     7561 gtctgggttg aatcgagtaa cggtcagcct tgattctttg gataacaaga tattcaagtc
     7621 gatgaacgat gtggacttcc cagtggagaa agttcttgaa ggcatggatg ctgctaaagc
     7681 cgctgggttg gagccgatca aggttaatat ggtggtgaag cgcggtgtga atgaagacag
     7741 cattttgccc atggcgcgct tcttccgtga aaaaggtttt attctgcgtt tcattgaata
     7801 catggatgtg gggagtacca atggctggcg tatgaacgat gtggtttcgg caaaagagat
     7861 cattagcatc atcaatgctg aaatgccgct agagcctgct gacccgaatt accgcggaga
     7921 agtggcagaa cgttggcgtt ataaagatgg gcagggtgaa attggtgtga tcgcttcggt
     7981 gacacaagcc ttttgccgtg attgcaatcg tgcgcgcctt tctgcagaag ggcaacttta
     8041 tacctgtctc ttcgcagcca aaggacatga cttccgcgat atgcttcgga atggcgccag
     8101 tgacgaagag atcttgcata agatcgaaac tgtttggggc aaacgcactg accgatattc
     8161 tgaaattcgt tctgataaca ccattccgct tccaaaagta gaaatgtctc atattggcgg
     8221 ttaggcgttt tcattctcta ttgttcttaa cataaaaaag acctcccgta ttttacagga
     8281 ggtctttttt attcaacttg tgaactagat gatttccagt tctttgagtt tggattcggg
     8341 tacaacgccg ttcacccatc cgcgcttctc gtagtattcc acgagcatgt cgtcgagttc
     8401 gcagacctga cccttgctgc caccgtaggt ggagggttca tcggtgaagc gcttcggcag
     8461 ggtgtcagag ccttcgcgga agccgttgag gttgttgatg tggcgttcga ggttgtaaac
     8521 gcgttcgccg accttgagca ggtgacccgc atcacagggg acgcccgtca tggcttcgta
     8581 ttgcatggcg aaggagtcga gggattcggc gaaggtggcg aatttgcaga tgtcgaggca
     8641 gtcggtgacg gcgtgaacgt tctggaagat gaagttcatt tcgcctttgc ccttccacgc
     8701 gagcgggtcc gcaacggtgg acggtccgag cacgttacca acgacttcag cggcgggggt
     8761 gtaaccacga aggtggcagg cgccgcgatt ggatgtggca tagcccaagc ccataccctt
     8821 gataccacga ggatcatacg caggcacgcc ctgccccttg acggtcatgg cgagttcggg
     8881 gtgaccaaag tgagcggcaa ccgcattcgc gccatcggca aggatgttgc c
//
LOCUS       DAOQNJ010000034         8840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3922_length_8840_cov_10.793170, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000034 DAOQNJ010000000
VERSION     DAOQNJ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3922_length_8840_cov_10.793170"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLG52_01940"
     CDS             <1..564
                     /locus_tag="PLG52_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNB40228.1"
                     /translation="KKTDLTDFKTCQVLGVFMEKNLEPIVKDLQTKFGATYEEFRGEV
                     QCMVKAENIVDVLKTLRDEHSFELLSVLNAIDYYPQTSPRFHVVYQLNSLSKNLRVQV
                     RVPVNDDQPKVPTVTGVFKTGMWREREVFDMFGIEFEGHPDPRRILMPEGTEGHPLRR
                     DFPLGYEEPQFTFNLEEIDLRKPYAKE"
     gene            568..1797
                     /gene="nuoD"
                     /locus_tag="PLG52_01945"
     CDS             568..1797
                     /gene="nuoD"
                     /locus_tag="PLG52_01945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNB40229.1"
                     /translation="MTQLQEVSVEQFKHLVSERALTGETMLLNMGPQHPSTHGVLRLL
                     LELDGETVVNCIPDIGYLHTGIEKNMEAKTYQKAEVMTDRLDYMNPMGNNLSYVMAVE
                     KLVDLDVPPRAQALRVILTELQRISSHLVLIGTWGLDLAAMSMFLYCFREREQILDIF
                     ELVSGQRMMTTFFRPGGLWRDVPVEFEKAVRDFIKIFPKRIDEYEKLLTKNPLFVDRM
                     VDIGYLSKETALSYGVTGPNLRASGVNWDLRKARPYMGYEQYDFDVPVLTEGDTYARY
                     LVRIEEFRQSLRIIEQALDKLPMGPVRSENRKFVPPPRSEIGVSMEALIHHFKLWTEG
                     FHAPDASIYSAVESPRGELGVLLAGDGGPKPRRVHMRTPSFDNLSVIPEIVKGHLVAD
                     LVAILASIDIVLGDIDR"
     gene            1794..2288
                     /locus_tag="PLG52_01950"
     CDS             1794..2288
                     /locus_tag="PLG52_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNB40230.1"
                     /translation="MSLEKKYSKEVKQILAKYPPEHKRSAVMPLLYLAQREEGYVNKE
                     SMKDIASILDITETEVASIIGFYSLYHDKKAGKYRMQVCNDLPCALRGSDEFLNKLCE
                     NLGVKVGETTEDGLITIEAVTCLASCDRAPMFQTQGPDGIKYHDYMTIDRTMELIEAL
                     KGGK"
     gene            2291..3538
                     /gene="nuoF"
                     /locus_tag="PLG52_01955"
     CDS             2291..3538
                     /gene="nuoF"
                     /locus_tag="PLG52_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNB40231.1"
                     /translation="MAYVLLRHRDIPDIEKMSVYKKNGGFSALKNAITKMQPNDVIDV
                     VKNSGLRGRGGAGFPTGMKWSFIDKNSWPHYIVANADESEPGTFKDREIMEKNPFQFL
                     EGLALGAYAVGANAAYIYLRGEFWQVAAWLDEKIAEMEEAGYLGENLFGKDYSLKIYT
                     HLGAGAYICGEETALLESLEGKRGQPRVRPPFPPSVGLYGKPTIINNVETFTNVPMIL
                     ANGADWYKSMGTADSAGVKIFSLSGRVRKPGNYELPFGTTFRQLIYEHGMGIQEGRPI
                     KAIMPAGASSSVIVVDEKVLDTPLDYASVRTFGADLGSASVIVLDDTVDMDWVINKTI
                     HFFKHESCGKCTTCREGNHWMRNLTDRIHSGKGSGDDVKLLLNVAKQMQGKCLCALGE
                     FSTMAVVTSIERFPDDFKKAVKA"
     gene            3545..6019
                     /gene="nuoG"
                     /locus_tag="PLG52_01960"
     CDS             3545..6019
                     /gene="nuoG"
                     /locus_tag="PLG52_01960"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNB40232.1"
                     /translation="MSKQVTLTINGKQVTAPEGLSVADAAKLAGIDIPVFCHHPKLEP
                     VGMCRMCLVEIGRPVRDRATGQFVMENGQPKIQFMPKLETACTNKVEDGMVVLTESAK
                     ATDGQRGTVEFLLTSHPLDCPICDKGGECPLQNLTMAHGPGKSRFNYNEKQHLEKMVP
                     LGELIYLDRERCIQCARCIRFQSDVAGEAVLGFDERGRYTQIISLSEPGFDSVFSGNT
                     TDICPVGALTTVDFRFGARPWELKAESSICTQCPVGCNTTLNTRREAKADGEVVVKRV
                     MPRQNEEVNEIWLCDKGRFAYHYTEGKERLTKPMVRKDEKLARASWDNTIKTAAENFV
                     ANKKDFVVLASGRLSNEDLFNLKSLADTAGGEAILYSDMAGGELTQLVGVGAGTNLGK
                     MGKGDAILVIASDLYEEAPIWWLRVKQAADRGATLIVANPRQTKLDRFAKYTIRYAYG
                     DEIKTIHDFEKKSKISDEFAKAKNAVVFYGSEGLGVAGTSALASACADLLKETNHIGN
                     ANNGLIGVWQRANDQGAFEVGFRPVADLEKALKGKAVYIVGADPVGDDPNLAKALKSA
                     KFVAVQELRETATTEIADVVFPAQAFTERDGTVVSGERRVQRFYAALAPKGDSKPDYA
                     ITSMLAKQMGVILQGTSASVVFDILANSVEAFNELTYEALAQVKEQWPLVGRNDVYYG
                     GTTYENTKGLGVQLVPVAQAGVSVKIQKVRKEAALRPKEKELLAVPANKLYDLGVTVQ
                     TAEFLDQRIGETKVAMHPTTAKKFEVEDGQAVKVSFEGVQAEAVARLDETIATGVVLI
                     PRSMGIAIHEPVVARVQAKTTSGKAK"
     gene            6022..7191
                     /gene="nuoH"
                     /locus_tag="PLG52_01965"
     CDS             6022..7191
                     /gene="nuoH"
                     /locus_tag="PLG52_01965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNB40233.1"
                     /translation="MDWTMWVEWVIKGFVLCLILLTGFAYLTLYERRALARIQVRVGP
                     NRAGPQGLLQPIADAVKLIFKEELTPGHVYKLVFILAPVLTVVPSLIIAAVIPLGTSF
                     SLFGRNINLYVANLNVGVLYLTSIASIAVYGIVLAGWSSNNKYAMLGGIRSSAQMISY
                     ELSLGLCFVIAIILSALGNPDGGSMNLREIVEAQKDMWFVVYQPAGALIFLIVTLAEV
                     NRAPFDMPEAEQELTAGYHAEYSGMKFALFFMAEYQKMIVISMIAATLFFGGYREFWF
                     MTNLFGGIFSVDRFWFLGPIYLMLKVVALLFFMIWVRATWPRIRYDRLMAFGWKVLLP
                     LCLILAFVTAVGILLVDFVGNPLIMWGVPVISVVFLFAFVAFINRDLRRKSHGRV"
     assembly_gap    7228..7327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7477..7998
                     /locus_tag="PLG52_01970"
     CDS             7477..7998
                     /locus_tag="PLG52_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNB40234.1"
                     /translation="MSSELIVFLVLSLVAIATALGMLFSQNAVYSALFLVLNFVTVAI
                     FYLLLGAPFIAMAQITVYAGAIMVLFLFVIMLLGAENLAPTNNLPWQKPLASLLSVIL
                     AVEAIYILLAKTSATTAITAPDAALNTMDSLREMALTLFGKFLLPFEVTSILLLVAMV
                     GAIVLTRQEREAK"
     gene            7998..8318
                     /gene="nuoK"
                     /locus_tag="PLG52_01975"
     CDS             7998..8318
                     /gene="nuoK"
                     /locus_tag="PLG52_01975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNB40235.1"
                     /translation="MVSVNYYVVLSAILFTIGALGVLSRRNPLIIFMSIELMLNAANL
                     AMVAFARSFEIAQGQSLFDGQIFVFFVIAVAAAEVAVGLALIVEIFKSKKNINIDEMN
                     SLKG"
     gene            8368..>8840
                     /locus_tag="PLG52_01980"
     CDS             8368..>8840
                     /locus_tag="PLG52_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40236.1"
                     /translation="MHLSETVNTGFYFLAPGLVLVPILGLLINAIFGKNFSEKMVGTV
                     ASLASGTAFVISVLLAYSVSINHGHAVSVPFAEWIHIGNLKLDWTFRVDSLSATMMLV
                     VSGVGTLIHIYAIGYMHEDVRFKHEEGRFNRFFLYLNLFIAAMMILVSGDSYLML"
BASE COUNT         2061 a         2075 c         2276 g         2328 t
ORIGIN      
        1 aaaaaaacag acctgacaga tttcaagacc tgtcaggtct taggtgtatt tatggaaaaa
       61 aacctcgaac ctattgtaaa agatctgcag acgaaattcg gcgcaacgta cgaagagttc
      121 cgtggtgaag tgcaatgtat ggtgaaagcc gagaatatcg ttgacgtgtt aaagaccttg
      181 cgcgacgaac attccttcga attactctca gtgcttaatg ccatcgatta ctacccacaa
      241 acctcacctc gttttcatgt tgtgtaccag ctcaattcac tctcaaaaaa tttacgggtg
      301 caggtgcgag tccctgtcaa cgatgaccag cccaaagtcc cgaccgtgac cggcgtgttc
      361 aagaccggca tgtggcgcga acgtgaagtc ttcgatatgt tcggcatcga gttcgaaggt
      421 catccagacc cgcgccgcat tctgatgccc gaaggcaccg agggacaccc gctgcgcaga
      481 gacttcccgc ttggctacga agaaccgcaa ttcactttca acttggaaga gatcgacctt
      541 cgcaagccat atgcaaagga ataatttatg acacaactac aagaagtgag tgtagagcaa
      601 tttaaacatc tggtttcaga gcgcgcgctg accggcgaga ccatgctctt gaacatgggt
      661 ccgcaacatc catccacgca tggtgtgttg cgtttgttgt tggaactcga cggcgaaaca
      721 gtggtgaact gtattcctga tatcggctat ttgcataccg gtattgaaaa gaatatggaa
      781 gccaagacct accaaaaagc ggaagtgatg accgaccgcc ttgattatat gaaccccatg
      841 ggaaataacc tctcctatgt gatggctgta gaaaagttgg tagacctgga tgtgccgccg
      901 cgtgctcaag ccttgcgcgt tatccttaca gagcttcaac gtatttcctc tcacctcgtg
      961 ttgatcggta catggggctt ggatctggca gccatgtcca tgttcttgta ctgcttccgc
     1021 gagcgtgaac agatcctcga tatttttgaa ctggtctctg gtcaacgcat gatgaccaca
     1081 ttcttccgcc ccggcggttt gtggcgcgat gtgcctgttg aatttgaaaa agccgtgcgc
     1141 gatttcatca agatttttcc taagcgcatt gatgaatatg aaaaactttt gacgaagaat
     1201 cctctgtttg tggatcgtat ggtggatatc ggttacctga gcaaagaaac ggctctttct
     1261 tacggggtaa cgggtccgaa cttgcgagcc tcaggcgtca actgggacct tcgcaaagcc
     1321 cgcccctata tgggctatga acaatatgat ttcgatgtgc cggttttgac cgaaggcgat
     1381 acctacgctc gttacttggt gcgtatcgaa gaattccgtc agtctttgcg cattattgaa
     1441 caggcgttgg ataaattacc gatgggaccc gtccgatcgg agaaccgcaa gttcgttcct
     1501 cctccccgtt cagagatcgg cgtaagcatg gaagcgctca tccaccactt caagttgtgg
     1561 accgaagggt tccatgctcc agatgcttct atttatagcg cagtggaatc cccacgcggt
     1621 gaactcggtg tgttgttggc aggcgatggc ggacccaagc cgcgccgcgt ccacatgcgc
     1681 actccatcat tcgacaacct ttccgtcatc cctgaaattg tcaagggaca tttggttgcc
     1741 gatctggttg cgatcctcgc ttccattgac atcgttctcg gagatattga ccgatgagtc
     1801 ttgaaaagaa atactcgaag gaagttaaac aaattcttgc caaatacccg ccagagcaca
     1861 aacgctctgc ggtgatgccg cttctttacc ttgctcagcg cgaggaaggc tacgtcaaca
     1921 aagagtcgat gaaagacatc gcctccattc ttgatattac cgagacagaa gtcgcttcca
     1981 ttatcggctt ctattctttg tatcacgata aaaaagcagg gaaatatcgt atgcaagttt
     2041 gcaacgattt accttgcgca ttgcgcggct cagatgagtt tctgaataaa ttgtgcgaga
     2101 acctcggcgt taaagtgggg gagaccactg aagatggtct catcaccatt gaagctgtga
     2161 cttgtctcgc ctcttgcgac cgtgccccca tgttccaaac tcagggaccg gatggcatca
     2221 aatatcacga ctatatgacc attgatcgta ctatggaatt gatcgaagct ttgaagggag
     2281 gtaagtaaat atggcttacg tccttttacg ccatcgcgat atccctgata tcgaaaaaat
     2341 gagtgtctat aagaagaatg gtggtttctc tgctctcaag aacgccatca ccaagatgca
     2401 gccgaatgat gtcatcgatg tggtcaagaa ctccggtctg cgcggacgtg gcggtgcggg
     2461 cttcccgacc ggcatgaaat ggtccttcat tgataagaac tcttggccgc actacatcgt
     2521 ggcaaatgca gatgagtctg agccgggtac gttcaaagac cgcgagatca tggaaaagaa
     2581 tcccttccag tttttggaag gtctggcgct gggtgcttat gcagtcggcg caaatgctgc
     2641 ctatatttat ctgcgcggtg agttctggca ggttgctgcc tggctcgatg aaaagatcgc
     2701 ggagatggaa gaagccggtt atcttggtga aaaccttttc ggcaaagatt attctctaaa
     2761 aatttatacc cacttgggcg ctggtgctta catctgtggc gaagagactg ctttgctcga
     2821 atcccttgaa ggcaaacgcg gacaaccccg tgtgcgcccg ccattccctc cttctgtggg
     2881 tctgtatggc aagccaacca ttatcaataa cgttgagacc ttcaccaatg ttccaatgat
     2941 tcttgccaac ggcgcagact ggtacaagag catgggcact gccgatagcg ccggtgtgaa
     3001 gatcttttca ctttcaggtc gtgtgcgtaa gcccggcaat tatgaattgc ccttcggcac
     3061 caccttccgc cagttgatct acgaacatgg catgggcatt caagaaggac gccccatcaa
     3121 ggcgattatg cctgcgggcg cttcttcctc tgtaattgtt gtggacgaaa aagtcctcga
     3181 cacgcctctg gactacgcct cggttcgtac cttcggagca gaccttggtt ccgcctcggt
     3241 cattgtgctc gacgatacag tcgatatgga ttgggtcatc aataagacca tccacttctt
     3301 taaacatgaa tcttgtggaa agtgcaccac ttgccgtgaa ggcaatcact ggatgcgtaa
     3361 cctgacagac cgcattcaca gcggtaaggg atctggtgat gatgttaaat tactcttgaa
     3421 tgttgccaag caaatgcaag gcaaatgttt atgcgcgttg ggtgaattct ccactatggc
     3481 tgtggtgacc agtattgaac gcttcccaga cgatttcaag aaagcggtaa aggcgtagca
     3541 gataatgagc aaacaagtta ctctcacgat caatggaaaa caagttaccg cgccggaagg
     3601 acttagcgtt gcagacgcgg caaaattagc agggatcgat attccggtct tttgccatca
     3661 ccccaaactt gaacctgttg gcatgtgccg catgtgtctg gtggagattg gtcgccccgt
     3721 gcgtgaccgt gctaccggtc agtttgtcat ggaaaatgga cagcccaaga tccaattcat
     3781 gcctaaactc gaaaccgctt gtactaacaa agtggaagac ggtatggtgg ttcttacaga
     3841 atctgccaag gcaacggatg gtcaacgcgg tacagttgaa ttcttgttga catcccatcc
     3901 attagactgc ccgatctgtg acaagggcgg ggaatgtccg ctgcagaatt tgactatggc
     3961 acatggtccg ggaaagagcc gcttcaacta caacgagaaa cagcacctcg aaaagatggt
     4021 tccgctcggt gagttgatct atcttgaccg cgaacgctgc atccaatgtg cgcgttgtat
     4081 ccgcttccaa agcgatgttg caggcgaggc ggttcttggc ttcgacgaac gcggacgcta
     4141 cactcaaatc atttctctct ctgaacccgg tttcgactcg gttttctcag ggaatacaac
     4201 cgatatctgc cccgtgggtg cgttgaccac agtggacttc cgctttggtg ctcgcccgtg
     4261 ggaacttaag gcagagtctt ctatttgtac acaatgccca gtgggatgca ataccaccct
     4321 caatacccgt cgtgaagcca aagccgatgg ggaagtggta gtgaaacgtg tcatgccgcg
     4381 ccagaacgaa gaagtgaacg aaatctggct gtgcgataag ggacgctttg catatcacta
     4441 taccgaaggc aaagaacgcc ttaccaagcc gatggttcgc aaagatgaaa agcttgcccg
     4501 tgcttcatgg gataacacca tcaaaaccgc agcagagaac tttgttgcga ataagaaaga
     4561 ctttgtggta ttggcttcag gtcgtctttc gaatgaagac ctgttcaacc tcaagtctct
     4621 tgccgatacg gctggcggcg aagccatttt gtactctgat atggcgggcg gcgaattgac
     4681 ccagcttgtg ggcgttggtg ctggcaccaa tctcggcaaa atgggcaaag gcgatgctat
     4741 tctcgtaatc gcttctgact tatatgaaga agctcccatt tggtggctgc gtgtgaagca
     4801 agctgcggat cgcggcgcga ctttgatcgt tgccaaccct cgccagacca agctcgaccg
     4861 ttttgctaag tacaccatcc gctatgcgta tggcgatgaa atcaagacca ttcatgactt
     4921 tgaaaagaag agcaagatct cagacgaatt tgcgaaggca aagaatgcgg tcgtcttcta
     4981 tggcagtgaa ggtcttggcg tggctggaac ctctgcactc gcttcagctt gcgcagacct
     5041 gttgaaagaa accaatcata tcggcaatgc caacaatggt ttgatcggtg tttggcagcg
     5101 tgccaatgat cagggtgcgt tcgaagttgg cttccgtccg gtggctgacc ttgaaaaggc
     5161 attgaagggc aaagccgttt acattgttgg agcagacccg gtgggggatg accccaacct
     5221 tgccaaagcg ttgaagagcg ccaaattcgt agccgtgcag gagttacgtg aaactgctac
     5281 aactgaaatt gcagatgtgg tcttcccggc gcaggcattt acagaacgtg atggtacggt
     5341 tgtctctggt gaacgccgtg tacaacgctt ctatgcggcg cttgctccca aaggtgatag
     5401 caaaccagac tatgccatca cctccatgct tgccaagcag atgggcgtga ttcttcaggg
     5461 aacttctgct tcagtagtgt tcgacattct tgcgaattct gtggaagctt tcaatgaatt
     5521 gacctatgaa gccttggctc aggtcaagga acaatggccg ctcgtgggtc gtaatgatgt
     5581 gtattacggc ggcacgactt atgagaacac gaagggcttg ggtgttcaac tcgtcccggt
     5641 ggctcaggct ggggtttcag tgaaaattca aaaggtccgc aaagaagcgg ctcttcgtcc
     5701 caaagaaaaa gaattgctgg ctgtgcctgc caataaattg tatgacctcg gtgtgactgt
     5761 ccaaaccgca gagtttcttg atcagcgcat tggcgagacg aaagttgcaa tgcacccaac
     5821 cacagcgaag aagttcgaag tggaagatgg gcaggctgtg aaggttagct ttgagggtgt
     5881 tcaggcagaa gcggtagcca ggctcgatga aactattgcg acgggtgtgg tgcttattcc
     5941 acgcagcatg ggcattgcga tccatgaacc tgtggtggcg cgagtgcaag cgaagaccac
     6001 atctgggaag gcgaagtaat tatggattgg acaatgtggg ttgaatgggt catcaaaggc
     6061 ttcgtgcttt gtttgatcct tttgacgggg tttgcctatt tgacgttgta tgaacgccgc
     6121 gcgctggctc gtattcaggt gcgtgttggt ccgaaccgtg cgggtccgca aggtttgttg
     6181 caaccgatcg cagatgccgt gaagttgatc ttcaaagaag aattgacgcc ggggcatgtg
     6241 tataagttgg tctttatcct tgcgcctgtg ttgacggtgg taccttcgct cattattgcg
     6301 gcagtcattc cgctgggtac ttcattcagc cttttcggac gtaatatcaa tttgtatgtc
     6361 gcgaatctga atgtaggtgt gctttatcta acgtccattg catcgattgc ggtgtacgga
     6421 attgtgctgg caggatggtc gagcaataac aagtatgcca tgttgggcgg catccgttct
     6481 tcggcgcaga tgatctctta tgaactttct ttaggcttgt gctttgtgat cgccattatc
     6541 ttgagcgctc tcggcaaccc cgatggcggc tcgatgaatt tgcgcgagat cgtagaagcc
     6601 caaaaggata tgtggtttgt ggtttatcag ccagcgggcg ctttgatctt tttgatcgtg
     6661 acgcttgctg aagtgaaccg cgctccgttc gacatgcccg aagcggaaca agaattgacg
     6721 gctggttatc acgccgaata ctcgggtatg aaattcgctt tgttcttcat ggcggaatat
     6781 caaaagatga tcgtgatctc catgatcgct gccaccttgt tctttggcgg gtatcgtgaa
     6841 ttctggttta tgacaaattt gtttggcggc atcttcagtg tggatcgttt ctggttcctt
     6901 ggaccgatct acttgatgtt gaaggtagtt gccttgttgt tcttcatgat ctgggtgcgt
     6961 gctacctggc cccgcatccg ctacgaccgc ttgatggcat tcggctggaa ggtgcttttg
     7021 ccattgtgct taatcctggc ttttgtgaca gcagtaggca tcttactggt tgactttgta
     7081 ggtaaccctc tcattatgtg gggtgtgcct gtgatttcgg tcgtttttct ttttgcgttt
     7141 gttgcattta ttaaccgcga tctgaggaga aaatctcatg ggcgtgtttg atcccattat
     7201 cgaactgaca aaaggtttgg gcgaaacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnccg agtccatgct gaccccgcgc aaagtggatg ctggtgtgtt cacccgttcg
     7381 gtgccggaga tgaaagaccc aaccgattga cgatttacga tttcagattt tcgattggtt
     7441 tggaaatcgt cagtcgaaaa tcgtaaatgg agtgccatgt cttctgaatt aatagtcttc
     7501 ctcgttcttt ccctcgtcgc gatcgcgacc gcactgggaa tgttgttcag ccagaacgct
     7561 gtatattccg ccttgttcct ggtgttgaac ttcgtcactg tggcgatctt ctacctcttg
     7621 ttgggtgcgc cattcattgc catggcacag atcacggtct atgctggtgc gatcatggtg
     7681 ttgttcctct tcgttatcat gctcttgggg gcagagaatt tagcccctac caataatttg
     7741 ccctggcaga aaccgctggc gagtctcctt tcggttattc tcgcagtgga agccatctac
     7801 attttgcttg caaaaaccag cgctacaacg gcgattacgg ctccagatgc agccttgaac
     7861 accatggata gtctgcgtga aatggcatta accctgtttg gtaaattcct tttgccgttt
     7921 gaggtgacat ctattttgct gttggtcgct atggtgggcg ctatcgttct aacccgtcag
     7981 gaaagggagg cgaagtaatg gtctctgtca attattacgt ggttttatct gccatccttt
     8041 ttaccattgg cgcgctcggt gtgctttcgc gccgtaaccc gctcattatt ttcatgtcca
     8101 ttgagctaat gctcaatgcc gctaaccttg ccatggtggc atttgcccgt tcatttgaaa
     8161 tcgctcaagg acaatctttg tttgatggac agatcttcgt cttcttcgtc attgctgtag
     8221 cggcggctga agtggcagta ggtctggcat tgatcgtgga aattttcaaa tccaaaaaga
     8281 atatcaacat cgatgagatg aattctctaa aaggataatt cgatattcga tactcgcaga
     8341 atcgggtatc gggttacgga gaatgcaatg cacttaagtg aaacagtaaa cacagggttt
     8401 tattttctag cccccggatt ggtgctggta ccgattctcg gtctgctcat caacgccatc
     8461 ttcggaaaga atttttccga aaagatggtc ggcacggtgg caagcctggc atcgggaacg
     8521 gcatttgtca tctcggtgct gctggcatat tcggtatcga tcaatcatgg tcatgccgtg
     8581 agcgtgccct tcgctgaatg gatccacatt ggcaacctta aacttgattg gaccttccgc
     8641 gtcgactcgc tttcggcgac gatgatgttg gtcgtttcgg gtgttgggac tctcattcat
     8701 atctatgcca ttggatacat gcacgaggat gtgcgcttca aacatgaaga aggacggttc
     8761 aaccgcttct tcctttattt gaacctgttc atcgctgcca tgatgatcct cgtctcaggc
     8821 gactcctacc taatgctctt
//
LOCUS       DAOQNJ010000035         8763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_3972_length_8763_cov_9.717042, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000035 DAOQNJ010000000
VERSION     DAOQNJ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3972_length_8763_cov_9.717042"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLG52_01985"
     CDS             complement(<1..395)
                     /locus_tag="PLG52_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40237.1"
                     /translation="MMKRNQPILMFVAVLTIAALACNLPTTSNPADATEEPTEVSTEA
                     PEATQTEAPTEEASDSVSVSVSTATNCRTGPDVVYQLLMTVQPGSDFDVVGKYTPKGY
                     WIINMPTGGTCWLWGQYAIVQGDTSALPE"
     gene            573..1001
                     /locus_tag="PLG52_01990"
     CDS             573..1001
                     /locus_tag="PLG52_01990"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNB40238.1"
                     /translation="MQPSDIPNEKTAKRIGNQASFIILALVFSCVVSFFFLFNVDKIK
                     MLQTSIDLQFNGSTTTGTVISLEEFKSSAGHNAVFILTAEFEVDGQPYTVTGNAYYPT
                     LDHSWQGESLDVIYDPQDPNIAMINTFEERWVIPLAGALP"
     gene            1029..1838
                     /locus_tag="PLG52_01995"
     CDS             1029..1838
                     /locus_tag="PLG52_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40239.1"
                     /translation="MKSDVIFPLTRIIAGIVVLFLVAAFGILFFLPDQTGTLFAWAIK
                     PHMSSMFFGSAYLGGAWILAQTAFGPRWHRVQAVFPAVTIFTTAMLAATLPHWERFSL
                     GTIPFLAWLILYIVSPFLIPALWIYNRNTDSHEPEESDVTVPTTARLILRLFGGLVLL
                     LVTICYLIPSLIIPIWPWELTPLTARVLCGWLSVIGTLSLVNSFDPRWTSWRASFEGI
                     FIAYSLIFVAAFMNPAEFKTGIINWYTLFTAGMLIAIVIFYGIMESRRRKR"
     gene            complement(1835..>3346)
                     /locus_tag="PLG52_02000"
     CDS             complement(1835..>3346)
                     /locus_tag="PLG52_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB40240.1"
                     /translation="YLNEVAAKWLGGTPQELVGKTMFDLFPEPMALQQMEGIREVFQE
                     NQSKVSESLTYIQGQYRWFHNSLQPIHNELGEVVNVLLHAVDVHELKVVQQELADLNR
                     SLEQRIEERTIEVRQTNLALERALLAKDEFLANMSHELRTPLNGILGMAEILLEEEYG
                     LLNERQHAYVNLIDSSGHHLLSLINDVLDLSKVEVGKLELDTEKVFIEDLCQSSMVFV
                     RQLAKKKNVLVNYLPDPAISTLQADARRLKQILVNLLSNAVKFTPEGGKVMLEVHANR
                     DEGIVTFSVTDTGIGISEDNQKRLFQPFTQVDSSLSRKYDGTGLGLALVKRLTELHGG
                     TVALQSEVGHGSCFTVSLPWREAGSTSHKLDGADAGDNHKSAPSAQTIHATVLLVEDN
                     TYNVMAISEYLEAKGYRLVFAVDGLDALAKAAENSPDLILMDIQMPNMDGLEATRRLR
                     ADPAFASIPIIALTAHAMGGDRERCLAAGATDYLSKPVKLKELESTIRKWLSS"
     assembly_gap    3347..3446
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3447..5475)
                     /locus_tag="PLG52_02005"
     CDS             complement(<3447..5475)
                     /locus_tag="PLG52_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB40241.1"
                     /translation="MIQAKPTYQELEQRCLTAESALAAIRSGQADTVLGEQGVLVLRL
                     KEAEKALQEAELKYRTLAENSPDIIYIIDLTKNKSGYLNRETLLGYTLEELERPGEFL
                     LSKIHPDDLSAVKAHWQAALAGNMNAEMEYRLQLKDGSWEWLQSRPRILSWDVNGRPT
                     QLLVTLSIITERKQAEKRLRSMLDIAQTMSTSLDTKTTLQQIVDSITQVTGLDSGAIY
                     TLTGNQLYLATTAPALPPEMPDQFRFANLADHPHIQTTLAKGSSVIIPDTEVAELTEA
                     EREICITRGLRSIAYLPLMSARTAIGVLIVASTKKLRTFTDEELLLYHGFSVQAAQTI
                     ENAQLYDSARVHSNELEQQISERKQAEKKLQESEKLYRYAMEVAGTVPYYESYSEKHP
                     AVTYEFIGEGIRQITGYGPEEFNSQIWDSLVEKAIPVEELEGYSLDEAIQRTRSGEFP
                     IWKCEFRLHDRDGKTHWVFEAAVELRDANGNPVGSIGSYQDITIRKQAEEKLRKSEEQ
                     YRLLFEVASDGVVLHLLSTDRIKNRFRRFNQNICQMLGYTAEELAQLSPMDIQQPEEL
                     LKVPGEAEQLEQNRELRFEKVFRRKDGSVFPSEVHSSVFELDGELLVLSIIRDISERK
                     QAEAKLRESEEKYRGLMEAIDSVVATIDYNGRFLYLNEVAAKWLGGTPQELV"
     gene            complement(5507..5803)
                     /locus_tag="PLG52_02010"
     CDS             complement(5507..5803)
                     /locus_tag="PLG52_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HNB40242.1"
                     /translation="MTANFKPNVLKKRYALRLFVAGNATNSRIARENLEQLLARHPEH
                     EFEVEIVDLNVQPEFALDQGVFISPALQILEPSSGGIVYGNLSQKEVLEKVLNL"
     gene            complement(5793..7220)
                     /gene="kaiC"
                     /locus_tag="PLG52_02015"
     CDS             complement(5793..7220)
                     /gene="kaiC"
                     /locus_tag="PLG52_02015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HNB40243.1"
                     /translation="MATQTLKTPTKIKGLDEVLNGGVPTGGLTLLNGGPGTGKTLFGL
                     EFLVRGAQEGIPGILITFEEHEEALHHYATTFGWNTAMLEKQGLLSIIGARFDPEGLL
                     SGDFDLGGILAILRHKTEKLSAKQVVIDAPDVFLRLLDNRGKERAELYKLINWLRDEK
                     LTALMTVKADGGDVARSDYEFLDYLADCVIQLDQRVIEQVTTRRVRVVKYRGSAHGRN
                     EYPFAMTDQGIWIIPITETQLNHAALGAPFPTGVAGLDAYMSGGYLRASCNLVTGTSG
                     TGKTTFAASFARTSAANGKRVFYINFEESPDSMVSCMLSPGIDLRPAMEDGSLRFLSV
                     MPEAQGIEEHLIQAFRGIEELKPDIVIVDAISACRRMGSRNSAFDYLLRLIDHCKTIG
                     ITSLLTNLTDSVKETQEITGIDLSSMIDTVVILRNVEYNGQFQRELSVLKARGRQHSN
                     KVHKFQITDHGVSIVNSNIEDAHDR"
     gene            complement(7536..8243)
                     /locus_tag="PLG52_02020"
     CDS             complement(7536..8243)
                     /locus_tag="PLG52_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40244.1"
                     /translation="MLERLDLNLTLEREEYNKQLKALQAELHELAFQVYAQKRPVVIV
                     YEGMDASGKGGAIKRLTERIDPRGYVVWPIAAPSGEDKERHYLYRFWRRLPEQGQIAI
                     FDRSWYGRVLVERLEGFCTEAAWRRAYGEINQFERQLTDYGTIVFKFWMHISKQEQLR
                     RFKERTRTNYKAWKITDEDWRNRGKWEDYIVAAEEMLLKTSTPDAPWTLVEANDKYFA
                     RIKSLRTVAEKLRRALK"
     gene            complement(8236..>8763)
                     /locus_tag="PLG52_02025"
     CDS             complement(8236..>8763)
                     /locus_tag="PLG52_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40245.1"
                     /translation="PWMWRFWQKIPARGQMVAFDTSWYRRVLIDRLNKTIKKDELRAA
                     YQDIAEFEEMLIQDGAVILKFWLHISEADQKKRIKKLRKDKFTAWQVGEEDLLQNKHY
                     EKFTSYVEDMIARTDSPHAPWTIVESADRYYMRIKVFETIIASLETRLGLAVSANEKT
                     KRPRKTKEMKGAVNA"
BASE COUNT         2160 a         2177 c         1908 g         2418 t
ORIGIN      
        1 atctcgggta gtgcactggt atcgccttgc acaatagcat attgacccca cagccaacag
       61 gttccacccg taggcatgtt gatgatccaa tatcctttag gagtatattt cccaacgaca
      121 tcaaaatcac tgcccggttg aacggtcatc aataactgat agacaacatc tggacccgta
      181 cggcaattgg tggcggtgct tacggagaca gacacgctat ctgaagcctc ttcagtgggt
      241 gcttctgtct gtgtggcttc aggcgcttcg gtacttacct cggtcggctc ttcggtggca
      301 tcggctgggt tggaggtagt gggcaggtta caagctaagg cggcaatggt taaaacagcc
      361 acgaacataa gaataggttg atttctcttc atcattattg ctccatgaaa tgtattcgtc
      421 tcgtattata tgaagagtga aaaagacaaa catcccacag agggaggatg acttttggat
      481 ttatcacaaa tgcccccaaa ctcccattgc aaacattttg tttgttcgtg acggaacggt
      541 cttgttcgtg tttctttact aaggagaaca acatgcaacc atcagatatc cccaacgaga
      601 aaaccgccaa acgcatcggc aaccaagcca gctttattat tcttgctttg gtcttttctt
      661 gtgtggtcag ttttttcttt ctcttcaacg tcgacaagat caaaatgcta caaacctcca
      721 tagacctgca attcaatggc agtacaacca ctggaacggt tatcagtctg gaagaattca
      781 aaagcagcgc gggtcataac gccgttttca tcttaacagc agagttcgaa gtagatggac
      841 agccctacac cgtcacaggc aatgcctact acccaaccct cgaccatagt tggcaaggcg
      901 aatcgttgga tgtgatctat gacccacaag acccaaacat tgccatgatc aacacctttg
      961 aagagcgctg ggtcattcca cttgcagggg cgttgcccta ggtaaacttt atcaatacag
     1021 gaaccaagat gaaatcagat gttatcttcc cccttactcg tattattgcc ggaatcgttg
     1081 tattgtttct agtcgccgcc tttggtatct tattcttttt gccagaccaa accggcacat
     1141 tgttcgcatg ggcaatcaaa cctcacatgt cctccatgtt ctttggttcc gcttatttgg
     1201 gcggcgcgtg gattctcgct caaacggctt tcggtccgcg ttggcatcgg gtgcaagccg
     1261 tcttcccagc ggtaaccatc ttcaccacag ctatgttggc tgccacgctt ccgcattggg
     1321 agcgcttttc tcttggcacc attcccttcc ttgcctggtt gatcttatat attgtctctc
     1381 cgtttttgat tcctgctctg tggatataca accgcaatac tgattcgcat gagccagaag
     1441 aatcggatgt gaccgtccct accacggcgc gacttattct ccgcctcttt ggtgggcttg
     1501 tcctgcttct cgttacgatc tgttatctca tcccaagcct gatcatcccg atctggccct
     1561 gggagttgac tccgctcacg gcacgggtct tatgcggttg gctttcggtg atcggaaccc
     1621 tttcgttagt gaactccttc gaccctcgct ggacgagttg gcgcgccagt ttcgaaggca
     1681 tcttcattgc ttacagcttg atcttcgtcg ccgccttcat gaatccggca gagtttaaga
     1741 caggcatcat taactggtac accctattta cagctgggat gttgattgcc atcgttattt
     1801 tttatggcat catggaatct cgcagacgca aacgctaact cgaaagccat ttacgaatag
     1861 ttgattcaag ttcctttagc tttacgggtt tactcaagta atcggtagca cccgctgcga
     1921 ggcaacgttc ccggtctcct cccatcgcat gagccgtgag ggcaatgatc ggtatggaag
     1981 caaaggcggg gtcagctcgc aagcgacggg tggcttccaa accatccatg ttgggcattt
     2041 gaatatccat cagaatcaaa tccggagaat tctccgctgc cttcgctaga gcgtccaaac
     2101 catctacggc aaataccaat ctgtagcctt tggcttcgag atactcgcta atcgccatta
     2161 cgttataggt attgtcttcc actaataaaa cagtggcgtg tatcgtttga gcagaaggag
     2221 cagatttatg attgtctccg gcatccgcgc catctaattt atgtgaggta gaaccagcct
     2281 cacgccaagg caaagagacg gtgaaacaac ttccatgccc tacttcactt tgaagagcaa
     2341 ctgtaccgcc atgcaactca gttaatcgtt tcaccagtgc cagccctaag cctgtgccat
     2401 catattttcg gctcaagctg gaatccactt gcgtaaacgg ctggaataga cgcttttggt
     2461 tatcttccga gatgccgatc cctgtatcgg tgacagagaa ggtgacaata ccttcatccc
     2521 gattggcatg tacctcaagc atgaccttcc cgccctctgg cgtaaattta acggcgttgc
     2581 tcaataaatt aaccaaaatc tgtttgagcc gcctggcatc ggcttgtaac gtggaaattg
     2641 ccgggtcggg caggtaattc accagcacat tctttttctt tgccagttgt cttacgaata
     2701 ccatactaga ttgacaaagg tcttctataa aaactttttc agtatccagt tccaatttgc
     2761 ctacttctac tttggacaaa tcaagaacat cgttgatcag gctaagcaag tggtgtccac
     2821 tggaatcgat caagttcaca tatgcatgct gacgctcatt gagcaagcca tattcttctt
     2881 caagcaggat ctcagccatg cccaaaatgc cgttgagcgg agttcgaagt tcgtggctca
     2941 tgttggctag gaattcatct ttggcaagca aagcgcgttc taatgccaaa tttgtctggc
     3001 gcacttcaat cgttctttcc tctatacgct gttcaagaga tcgattcaaa tctgccaatt
     3061 cttgctgtac gaccttaagt tcatgaacat caacggcatg aagcaacaca ttaactactt
     3121 cgcccaattc attatggatc ggttggagtg agttatgaaa ccaacgatac tgcccctgaa
     3181 tataggtcag tgattcactc actttactct gattctcttg aaagacctca cgtatgcctt
     3241 ccatttgttg caatgccatc ggctcaggaa aaagatcgaa catggtctta ccgaccagtt
     3301 cttggggtgt gccacccagc catttggcag caacctcatt caagtannnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnncgac cagttcttgg ggtgtgccac ccagccattt
     3481 ggcagcaacc tcattcaagt acaggaatct cccgttgtaa tcaatcgtag ccaccacact
     3541 atcgatcgct tccatcaagc cgcgatattt ttcttcactc tcgcgtaact tcgcctccgc
     3601 ttgtttgcgc tcagaaatat cacggatgat ggaaagcacc agaagctcgc catctaattc
     3661 aaagacactc gaatggactt cggatggaaa tacggaacca tctttacggc gaaatacttt
     3721 ctcgaacctt aattcacgat tctgctccaa ctgttctgct tctccgggca ccttgagaag
     3781 ttcttctggc tgttggatat ccattggact caattgcgcc aattcctcag cggtatatcc
     3841 caacatctgg caaatatttt gattgaaacg ccggaaccga ttcttaatgc gatcagtact
     3901 tagcaaatgc agcaccaccc catcactagc tacttcaaac aataagcggt actgctcttc
     3961 actctttcgt agtttttcct ctgcttgttt acgaatggtg atatcttgat aagaaccgat
     4021 ggacccgaca gggtttccat tggcatctct taattccact gccgcttcaa aaacccaatg
     4081 ggttttccca tcgcgatcat gcagccggaa ttcacacttc cagatcggaa attcgccaga
     4141 gcgggttctt tgaatggctt catcaagcga atagccttca agttcctcta ccggtattgc
     4201 cttttccact aacgaatccc aaatttgtga attgaattct tcaggtccat agccagtgat
     4261 ctgacgaatc ccttctccaa taaattcata tgtaacggcg ggatgcttct cgctatatga
     4321 ctcgtaataa ggcaccgtgc cagccacttc catagcataa cgataaagct tctctgattc
     4381 ctgtagcttc ttctcggctt gtttgcgttc agaaatctgc tgttccaatt cattggaatg
     4441 aactctagca gaatcatata actgtgcatt ttcaatcgtt tgggcagcct gaactgaaaa
     4501 gccatgataa agaagtaact cttcatcggt aaatgtgcgt aatttctttg ttgaagcaac
     4561 aatcaatacc cctattgctg tacgagcact cataagcggc aagtatgcaa tcgaacgcaa
     4621 acctctagta atgcagattt cacgctcagc ttcggttagt tcggcaactt cggtatcagg
     4681 tatgatgaca gaagagcctt ttgcaagtgt agtttgaata tgggggtggt cagccagatt
     4741 ggcgaaacga aattgatctg gcatttcggg cggtaaagca ggcgcggtag ttgctaaata
     4801 gagttggttt cctgtcaggg tgtagatcgc accgctatct aaccctgtca cttgcgtgat
     4861 gctatcaaca atttgttgca aggtcgtttt tgtatcaaga gaggtggaca ttgtttgagc
     4921 aatgtctagc atagaacgta aacgtttttc cgcctgtttg cgttcagtga ttatactaag
     4981 tgtaactaat aattgagtgg gcctaccatt cacatcccaa ctcaagatgc gcgggcggct
     5041 ctgcaaccat tcccaactac catctttcaa ctgcaaacgg tattccatct ctgcattcat
     5101 gttgcctgca agtgccgcct gccaatgcgc tttcacggca gaaagatcgt cgggatgtat
     5161 tttggaaagc agaaactccc ccggtctttc caattcttcc aaggtatacc ccaacagtgt
     5221 ttcgcggttc aaataccccg acttgttttt cgttagatca ataatgtaaa taatatctgg
     5281 agaattttct gccagggtgc gatatttaag ttctgcttct tgtaaggctt tttctgcctc
     5341 tttcaggcgc aagaccaata ctccctgttc accaagtaca gtatcagcct gtcctgagcg
     5401 gatcgccgct aatgcggact cagctgttag gcagcgttgt tccaattcct gatatgtcgg
     5461 tttcgcctga atcaaggggc acgctccgtt tctttgcaaa accagatcaa aggttcaaga
     5521 ctttttccaa aacttctttt tgacttaagt tgccgtacac aatcccacca gaacttggtt
     5581 caaggatttg taaggcgggg gatataaaca caccctgatc caaggcaaat tcgggttgta
     5641 cgttgaggtc aacaatttca acttcaaatt catgctcagg gtgtcttgcc aacaactgct
     5701 ctaagttttc acgcgcgatc cgtgaattgg tggcattccc ggcaacaaac aagcgcaggg
     5761 cataacgttt tttaagaacg ttaggtttga aattagcggt catgggcatc ctctatattt
     5821 gaattcacta tagaaacccc atgatcagta atctgaaact tatggacttt atttgaatgt
     5881 tgacgtcctc gggctttaag cacagacagt tctcgttgga attgcccgtt gtattctaca
     5941 ttgcgcaaaa taactacagt gtcaatcatg gaagacagat caatgccagt gatttcctga
     6001 gtttctttta cactatcggt taggttggtc aacaggctgg taataccaat agtcttacag
     6061 tgatcgatca gacgcaacaa ataatcgaag gcagaattac gtgaccccat gcgtcgacaa
     6121 gccgagatgg catctacgat cacgatatcg ggtttcaatt cctcaatccc gcgaaatgct
     6181 tgaataagat gctcttcgat tccctgcgcc tcaggcatca cagataagaa cctcagagag
     6241 ccatcttcca tagctgggcg cagatctata ccggggctaa gcatgcaaga taccattgag
     6301 tctggtgatt cttcaaagtt gatgtaaaag acgcgtttgc cattggcagc ggatgtacgc
     6361 gcaaacgaag cggcaaaggt tgtcttaccc gtaccagatg tacctgtgac caaattgcag
     6421 gaagcacgca aatacccgcc gctcatgtat gcatccaaac cagccacacc ggtggggaaa
     6481 ggcgcaccca gcgccgcatg gtttagttgt gtttccgtga tcggaatgat ccaaatgcct
     6541 tgatcggtca tggcaaacgg gtactcattc cgtccatgtg cagagccgcg atacttaacc
     6601 acacgtaccc gacgggtggt gacttgttcg atcacacgtt gatcaagttg gatcacacaa
     6661 tctgcaagat aatcaagaaa ctcataatca gaacgggcaa catccccccc atctgctttg
     6721 accgtcatca gagcagtcaa cttttcgtca cgcaaccagt tgattagttt gtataactcc
     6781 gcacgctcct tgccacgatt atcaagcaaa cggagaaata catcgggagc atcaatgaca
     6841 acctgcttag ctgaaagctt ttctgttttg tggcgcaaga tcgccagaat gccgcccaag
     6901 tcaaaatcgc ctgagagtaa cccctccggg tcgaaccttg ccccaatgat cgaaagaagt
     6961 ccttgttttt caagcatggc agtattccag ccaaatgtag tggcgtaatg atgcaaggct
     7021 tcttcatgtt cttcaaacgt aattaggatg ccaggaatcc cctcttgtgc gcctcgcacc
     7081 aggaactcta gcccaaacaa agtcttgccg gtgccgggtc ctccgttcaa caaggtcagt
     7141 ccgccagtgg gcacaccccc attaaggact tcatcgagac cttttatttt ggtaggagtt
     7201 ttaagggttt gggttgccat aaaatcagca ttcctttcaa gagaatagga tctggttatg
     7261 tctcaaacaa ggctagtctg cttgcctaac gtatggaact atttcattgc aaatatgaca
     7321 ttacacttta atatgatcat ctctcgatac cgatttcatc gtctccccgc aatcctaacc
     7381 gcacaaacgt taaaaggcta aatttatttt atacacattt ttgcattttt acacatgctt
     7441 ttcattatga tttgagggtg ttttcctccc atttctcgga aaatagtgcg taaaacaggc
     7501 atccgatagg gtacggctca tgagggcata gcaatttact tcaacgcgcg ccgtaatttc
     7561 tcggcgaccg tgcgcaggga cttaatacgg gcaaagtatt tgtcgttggc ttctaccaat
     7621 gtccatggag catcgggggt gctggtcttc aagagcatct cttcggcagc gacgatgtaa
     7681 tcttcccatt tgccgcgatt gcgccaatct tcatcggtga tcttccaggc tttgtaattg
     7741 gtgcgggtgc gttctttgaa acggcgcagt tgttcttgtt tgctaatatg catccaaaac
     7801 ttaaagacga tggtgccgta atcggtgagt tgacgttcaa attggttgat ctcaccatat
     7861 gcgcgacgcc aagcggcttc ggtgcagaaa ccttcgaggc gttcgaccaa gacgcgccca
     7921 taccaagacc ggtcaaagat ggcgatctga ccttgctcgg gcaaacgtcg ccagaagcga
     7981 tatagatagt gccgctcttt atcttctcca gatggagcgg caatgggcca gaccacgtat
     8041 ccacgcggat cgatccgttc ggtgagacgt ttgatcgcgc cgcctttgcc ggaagcatcc
     8101 atcccttcat agacgatcac cacggggcgc ttttgggcat agacttgaaa tgccagctca
     8161 tgcaattcag cttgcaacgc cttgagctgt ttgttgtatt cctcgcgttc gagtgtcaag
     8221 ttaagatcga gtcgctcaag cattgacggc tcctttcatt tcctttgtct tgcgcggacg
     8281 ttttgttttt tcatttgcag aaactgccaa acccaaacgg gtttcgagcg aagcgatgat
     8341 ggtttcgaat accttgatgc gcatgtaata tcgatcggca gattcgacaa tcgtccacgg
     8401 agcgtgaggc gagtctgtgc gggcgatcat atcttcaaca tagctggtaa atttttcgta
     8461 gtgcttattt tggagtagat cctcttcgcc cacttgccag gcggtgaatt tatctttgcg
     8521 taatttcttg atgcgtttct tttggtctgc ttcgctgatg tgcagccaaa acttcaagat
     8581 aaccgcccca tcttgaatca acatttcttc aaattcggca atgtcttgat aggcagcacg
     8641 caactcatct tttttgatgg tcttgttcaa gcggtcgatc agaacgcggc gataccatga
     8701 ggtatcgaat gcgaccattt gcccacgggc gggaatcttt tgccagaagc gccacatcca
     8761 cgg
//
LOCUS       DAOQNJ010000036         8714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4007_length_8714_cov_7.671556, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000036 DAOQNJ010000000
VERSION     DAOQNJ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8714
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4007_length_8714_cov_7.671556"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLG52_02030"
     CDS             complement(<1..775)
                     /locus_tag="PLG52_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNB40246.1"
                     /translation="MSNYDIKDLQLAEGGRRRIDWAEREMPVLRSIRERFKKEKPLKG
                     MRLSCCLHVTTETANLMITLQEGGADIVLTASNPLSTQDDVAAALVNNYEIPVFAIKG
                     EDKVTYFKHIRAALEHKPHMTQDDGADLVSSLFFIEMGRFEKLEPSLASWAKGLSEEE
                     RKQMLKNVIGGTEETTTGVIRLKAMEADGVLKFPVIAVNDALTKHMFDNRYGTGQSTI
                     DGIIRATNVLLAGKTFVVAGYGWCGRGLASRARGMGAHVI"
     gene            complement(972..1928)
                     /locus_tag="PLG52_02035"
     CDS             complement(972..1928)
                     /locus_tag="PLG52_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNB40247.1"
                     /translation="MDILLTGSVAYDYLMTFPGHFKEQILPERLESISLSFLVDSMSK
                     QRGGIAPNIAYTMALLGQKPRVMATVGEDFGDYRAWLEAKGVDTNLMKVVPGVFTASF
                     FATTDHASAQIASFYPGAMGYSATQSLKELDKKPDLVIVSPSSPDAMMKFPAEARELG
                     IPYLYDPSQQVLRLEGPELARDMEGAYFLFCNDYEFGLISKKTGWSLDQILNHVKVLV
                     ITRGKDGADLYSDGTSVHIPTVPEDEIVDPTGVGDAFRGGFLAGYSHGFDWKLCGEIG
                     SLSAVYCLEQRGTQNHSYTREDFVKRFRKHFDDGGRLDVLLK"
     gene            complement(2065..3216)
                     /locus_tag="PLG52_02040"
     CDS             complement(2065..3216)
                     /locus_tag="PLG52_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB40248.1"
                     /translation="MNTHHKIHRWFRLILSSVLLIAVTACAAGSAPASTDELVTANGF
                     VCPEPSPRVEFESDTINIFTWTEYVPADMIDCFGMVYDITVNVDYFSSNEELYSKVSL
                     GEDVNPYDVIHPSDYMIAVLMREGLLQELDPARLPNLANLDSGLVTAYGEATSYVVPY
                     QMGTQAIIYNSDTVETPPTSWADLWNPEYEGRIVSVDDSRVVIGAALLTLGYDVNDTD
                     PDHLEEAKQKLLELMPNIRVFDSDSPKTPLVAGDVDLGIVWNGEAFLTASEAPQFKYV
                     FPEEGSIIFYDGMGIPPSATHADAAYAWFNYMLQGDVNWLTLVDYPYTNPNKAALDYA
                     KENQPDIYEAYISSSITNTPADEFAKGHDVEDIGEALLMYDEIWTEIKQ"
     gene            3342..5768
                     /locus_tag="PLG52_02045"
     CDS             3342..5768
                     /locus_tag="PLG52_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB40249.1"
                     /translation="MKKLRLLLGLWLATSLVACSTLKNFPTIPPGWTVTPSTSPTPEA
                     TFTPTITPTPLPVARVSEGDKAFFNGDFDVALLHYQVAIQDSPDPLVRAAAEWGQAKI
                     YYAQGRYNETLTSLQTLISEYPQAPQHPQAYFLQGFVYYKLENYPAAADSWQTYLVLR
                     PGVLDAYVQELRGDALFNAKDFAGALASYTTAITAPSLGDDTTLDLKVASVQTQLGNY
                     EEAIALYDGIIGRATSDYTKAQALYEAGTVNLTQGQTAEANERFRYAIENYPLSYYSY
                     LSLVALLDAGGTVSDLDRGLVDYFSGQYAVGIAAFDRYLQSNPADNDGTAYYYRALCK
                     NALGQYDEAIADYQNFISNFSAHPRWGDAWGEKAFIEWAQKGDYTTGAQTLLEFINLA
                     PTSPQAAEYLMSAARIYERDAQYDKAIETWSRVVIEYPGSQQASYAAFLSGIVEYRRG
                     NHQASLDLFKRSLALSVSVEDKSRALLWIGKANQILGNQGPAEDAWREGQNLNPGGYY
                     SERARDLLVGQAPFATGSAQNYQVDYARERADADTWMRLTFNLGNDVDLSNLGPLASD
                     ARIIRGKEYWDLGMLDEARLEFEDVRNELETLNDAVGSYRLANYLVDLGMYRSAVFAA
                     RQVLTIAGLDEHTESMMAPAYFSHIRYGLYYSDLVLPTSQQEGFDPLFIFSVIRQESL
                     FEGFVRSNAGAHGLMQIIAPTGAQIASELGKPLNYSEEDLYRPYVSVLFGTHYLARNR
                     TLMNGDLYATLAAYNGGPGNAAAWKDLSGEDQDLFLESVRFEETRNYIRNIYEIFIVY
                     KRLYGTPITQ"
     gene            complement(5851..6159)
                     /locus_tag="PLG52_02050"
     CDS             complement(5851..6159)
                     /locus_tag="PLG52_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNB40250.1"
                     /translation="MSREYVLHDGKRGSALAVRITPRASRNQIAGVLNDGTVKIQLAA
                     DPDDKNLNSDLLAFLAEVLGVPRTRVEIVAGESGRDKLISVLDMDVETVHQRIVAHVE
                     "
     gene            complement(6169..6423)
                     /locus_tag="PLG52_02055"
     CDS             complement(6169..6423)
                     /locus_tag="PLG52_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNB40251.1"
                     /translation="MNGNTIISIIIIVADFFVWVVIASSLLSFFLPPYHPVREALGKI
                     VDPFLNPIRSLMPSGGGLDFSPLILILIIELITNFLRSLL"
     gene            complement(6420..7163)
                     /locus_tag="PLG52_02060"
     CDS             complement(6420..7163)
                     /locus_tag="PLG52_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNB40252.1"
                     /translation="MSELVNSIRERYLSALDTIASAANRSGRSPEAVKLVVVTKFQPL
                     EVVQAAIEAGAVILGENYAEEGVTKIQSLQNLSAVEWHMIGHVQSRKAPLVAGHFNFM
                     HSLDSLKLAKKLDRFCGEAGRVLPVLLEFNVGGEDSKGGWEAVDETRWEELLPEVSEL
                     LAMPNLQVRGLMSMPPLGMDAEFARPYFQKLRRLQEFFTSHLPQAKWIELSMGTSSDY
                     EVAVEEGATFVRIGTAIVGPRNYQVAENL"
     gene            complement(7193..7972)
                     /gene="pgeF"
                     /locus_tag="PLG52_02065"
     CDS             complement(7193..7972)
                     /gene="pgeF"
                     /locus_tag="PLG52_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNB40253.1"
                     /translation="MPFDQSDGLRYYQFDIFSKNILNAVFTRQGGISPEPWTSLNLSI
                     SVGDDPARVAENRVHAFNALGRNPASIHDAWLIHGTDVIFADAPRSLDAPTQKADILF
                     TDNPEVSLFMRFADCVPLLFHDPKKNVIGISHAGWMGTVKGVAEVSVQAMQERYGSNP
                     QDIVVGIGPSISADHYEVGEEVAAQFREKYGRDSEQVVQVRDEKIYLDLWAANALQLQ
                     KMGVEHIQISGLCTACHLDDWYSHRAEKGKTGRFGVLLAMV"
     gene            complement(7972..>8714)
                     /locus_tag="PLG52_02070"
     CDS             complement(7972..>8714)
                     /locus_tag="PLG52_02070"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNB40254.1"
                     /translation="PVYKYKMEKGKKLSTGDRVSFWISKRYLLLLNLFMLLYVGLPFL
                     APTLMKLGAEAPAQLIYRIYKPLCHQFAFRSFFLFGEQPFYPLAEANLTGYKTFEQAT
                     GIAGVDDPYNMARFEARNYIGDSTIGYKVALCERDVAIYLAILAFGIIFGLTGRRFKS
                     VHWLVWILIGIAPIGLDGFSQLFSQFNWDWLNTLLPYRESTPFLRVLTGALFGLFTAW
                     FAYPNIEESMNETRQYFLKKSAVIEAAE"
BASE COUNT         2367 a         2254 c         1982 g         2111 t
ORIGIN      
        1 cgattacatg agcacccata ccgcgagcgc gggaagcgag accacgtccg caccatccgt
       61 agccggcgac aacaaaggtc ttaccagcca acaaaacgtt ggttgcgcgg atgataccgt
      121 caatggtgga ctgaccggtg ccatagcggt tatcgaacat gtgcttggtg agagcatcat
      181 tcacagcaat gaccgggaac ttgagcacgc catcggcttc catcgccttc aagcggatca
      241 cgccggtggt ggtttcttca gtaccaccaa taacgttctt gagcatctgc ttgcgttctt
      301 cttcgctcaa acccttcgcc caagaagcga gagaaggttc gagtttctcg aaacgtccca
      361 tttcgatgaa gaaaagggag gaaacgaggt cggcgccatc gtcctgggtc atgtggggtt
      421 tgtgttccaa tgcagcgcgg atgtgcttga agtaagtgac cttatcttcg cccttgatcg
      481 cgaagacagg gatttcatag ttgttgacca acgccgcagc cacatcatct tgagtggaaa
      541 gcggattgga ggcagtgagg acgatgtccg cgccgccttc ctgaagggtg atcatcaggt
      601 ttgcagtttc ggtggtcaca tgcaaacagc aagacaggcg catacccttg aggggctttt
      661 ccttcttgaa gcgttcgcgg atggaacgca gcacaggcat ttcgcgttct gcccaatcaa
      721 tgcgacggcg tccgccctct gctaattgaa gatctttaat atcgtagttg ctcataaata
      781 ctccttaagt ctttatacaa ggaatgtgat ttggaaaccc atttgtgtcc gcataaaagg
      841 gttcaaatca cggactaata atttaattta ggttggtctc aacagttgac attgctttca
      901 agcggagcaa tgcgtatgta cggcaacatt ctgcgaacac cgccagctta accaacaaat
      961 ctaaatagat tttactttaa taagacgtcc agtctgccgc catcatcgaa atgcttgcgg
     1021 aaacgtttga cgaaatcttc gcgtgtataa gaatggttct gggtgccgcg ttgttcaagg
     1081 cagtagaccg cagaaagcga gccgatctca ccgcacaact tccaatcaaa gccgtgagaa
     1141 tacccggcga ggaacccacc gcggaaggca tcccccacac ctgtggggtc tacaatttca
     1201 tcttcaggca cggtgggaat gtgaaccgaa gtgccatctg agtagaggtc tgcgccgtct
     1261 ttgccgcgcg taatcaccaa gactttaaca tggttgagga tctggtcgag gctccagccg
     1321 gtcttcttgg agatcaagcc aaattcgtag tcattacaga acaagaaata tgccccttcc
     1381 atatcgcgag caagttctgg tccttcaagg cgcaagactt gctggctggg gtcatacaaa
     1441 tatggaatac ccaattcacg tgcctcagca gggaatttca tcatcgcatc tggcgaactg
     1501 ggagagacaa taacgagatc aggtttttta tcgagctctt tgagcgattg agtcgcagag
     1561 tagcccatgg ctccggggta gaacgaagca atctgagcgg aggcatgatc cgttgtcgcg
     1621 aaaaacgagg cggtaaacac gcccggaacc accttcatca ggttcgtatc cacgcctttt
     1681 gcttcgagcc aggcgcgata atcgccaaag tcttcaccta cagttgccat cacgcgcggc
     1741 ttttgtccca gcaacgccat ggtgtaggca atgtttgggg caatgccacc gcgctgtttg
     1801 gacatggaat caaccaagaa cgaaaggctg atcgactcca aacgttcggg gaggatttgt
     1861 tccttgaaat gacccggaaa ggtcatcaaa taatcatagg ctacagagcc agtaagaagt
     1921 atatccatgc agtttccttc tcgcagtaaa tggcgcactc ggttgagtgc gccataagat
     1981 atcgaacggg caaaagttta tcacggggtt ttatgaatgt aaagtttttg agcttacaaa
     2041 aataacccgt gcaattcgcc tactttattg cttaatttcc gtccaaattt cgtcgtacat
     2101 cagcaaggct tcgcctatat cctcaacatc atgtcctttt gcaaactcat cggccggggt
     2161 gttggtgatg gatgaagaga tataagcttc ataaatgtct ggctggtttt ctttggcata
     2221 atctaacgca gctttgttgg ggttggtgta cggataatcg accaaggtca gccaattgac
     2281 atcgccctgc aacatatagt tgaaccaggc atatgctgca tcggcatgag tcgctgaagg
     2341 cggaatcccc ataccatcat aaaagatgat cgacccttct tctgggaaaa cgtatttgaa
     2401 ctgcggagct tcagaagccg tcaggaacgc ttcaccattc caaacaattc ccaaatctac
     2461 atcgcctgcc accagtggag ttttggggct atcgctatcg aacacacgaa tgttcggcat
     2521 aagttccaac aacttctgtt tagcttcttc taaatggtct gggtcggtat cgttgacatc
     2581 gtaacccaaa gtcaacaaag cggcaccaat caccactcgg ctgtcatcca cagacacgat
     2641 gcgaccttca tattcagggt tccaaaggtc agcccaagaa gttggcggag tttccacagt
     2701 gtcactgtta tagataatcg cctgtgtccc catctgatac ggcacaacat aactagtcgc
     2761 ttcgccatat gccgttacaa gacccgagtc aaggtttgcc aaattaggca aacgcgcagg
     2821 gtccagttct tgcagtagcc cttcacgcat caaaacggcg atcatgtaat cgctggggtg
     2881 aattacatca taagggttta catcttcgcc aagcgaaact ttcgaataca gctcttcatt
     2941 tgaagaaaaa taatcaacgt taacggtaat gtcatacacc atgccaaaac aatcgatcat
     3001 atcggctggc acatattccg tccaagtgaa tatattaatc gtatcacttt caaattccac
     3061 gcgtggactc ggctctgggc aaacaaagcc attggcagtg accaattcat ccgtcgaggc
     3121 gggggcagac ccagcagcgc aggctgtcac ggcgatcagc agcacgctcg acaaaatcaa
     3181 ccgaaaccaa cgatggattt tgtgatgtgt gttcataatt ctccttaggg ttataaaata
     3241 atgtaacaaa gagattatag tgcaagctaa acgtgaatac aacattagaa atcataaatg
     3301 ccccgcttga cgcactcatc ctttaccagt ataaaaacgg gatgaaaaaa ctgcgcctcc
     3361 ttctcggact ttggcttgcc acatctctcg tagcttgttc tacgctcaaa aatttcccta
     3421 ccatcccccc cggctggacg gttactccca gcacatcccc cactcccgaa gcgaccttta
     3481 ctcccaccat tacgcctacg cctttacccg ttgcccgagt cagcgaaggg gataaagcct
     3541 tcttcaacgg tgattttgac gtagccttat tacactatca agttgccata caagattcac
     3601 cagacccctt ggtgcgtgcc gcagcagaat ggggtcaagc caaaatatat tacgctcaag
     3661 ggcgctacaa cgaaaccctc acatcccttc aaacccttat tagtgaatat cctcaagctc
     3721 ctcaacaccc acaagcttat tttctacaag gctttgtcta ttacaaactt gaaaactacc
     3781 ctgcggcggc tgattcctgg caaacatatt tagtcttgcg cccaggcgta ttagatgcct
     3841 acgtacaaga actgcgaggt gacgccctct tcaatgcaaa agattttgcc ggagcgttgg
     3901 cgtcttacac cactgcaatc actgccccat ctctcggcga cgataccacc ttagacttaa
     3961 aagtcgcatc tgtacaaaca caactcggca attatgaaga agcaattgcg ttatacgacg
     4021 gaatcattgg gcgagccacc agtgactaca ccaaggctca agcgttatac gaagctggga
     4081 cggtgaatct cactcaaggt cagaccgccg aggcgaatga acgtttccga tacgctatag
     4141 aaaattatcc actttcctat tattcctatc ttagccttgt cgctttgctt gatgccggtg
     4201 gaacagtcag tgacctagac cgtgggctcg tagattattt ttcaggtcaa tacgcagtag
     4261 gcatcgccgc attcgaccgc tacttacaaa gtaaccccgc agataatgac ggtacggcat
     4321 attattaccg tgcgctttgt aaaaacgcat tggggcagta cgatgaagcg atagcagatt
     4381 atcagaactt catttcgaat ttctctgcgc atccacgctg gggagatgca tggggtgaaa
     4441 aggcgtttat cgaatgggca caaaaaggag attacaccac cggcgctcaa acgctcttgg
     4501 agtttatcaa cctcgcccct acttccccac aagcagctga gtatcttatg agtgccgcac
     4561 gcatttacga acgagatgcc cagtatgata aagctattga aacatggtct cgtgttgtca
     4621 ttgaataccc tggttcgcaa caagcttcat atgcagcgtt tttatctggc atcgtggaat
     4681 accggcgcgg caatcatcaa gcatcgttag acctttttaa acgcagcctg gcactttcag
     4741 tttctgttga agataaaagc cgtgccctgt tatggatcgg caaagccaat caaattttag
     4801 gaaatcaagg tccagctgaa gacgcgtggc gtgaaggaca aaacctcaac cccggcggct
     4861 attacagtga acgtgcgcga gacctattgg tagggcaagc tccatttgca acaggcagcg
     4921 cccaaaatta ccaagtggac tacgccagag agcgagccga tgccgatacc tggatgcgcc
     4981 tgaccttcaa ccttggcaac gatgtagact tgagcaacct cggtccattg gcttcggacg
     5041 cacgcattat ccgtggcaaa gaatattggg atttgggcat gcttgacgaa gctcggcttg
     5101 aatttgaaga tgtacgcaat gaacttgaaa cactcaacga tgccgttggc agttaccgcc
     5161 tcgccaacta cttagtcgat ttaggcatgt accgttctgc tgtttttgcc gcccgccaag
     5221 tattgaccat cgcaggcttg gatgaacata ccgagtctat gatggcacct gcctatttca
     5281 gccacatccg ctacgggttg tattattcag atttagtcct ccctacttct caacaagaag
     5341 ggtttgaccc gttatttatc tttagcgtaa ttcggcaaga aagtttgttt gaaggctttg
     5401 tccgctcgaa cgcaggcgcc catggactca tgcagattat cgcccctacc ggagcgcaaa
     5461 tcgcctcaga actcggaaag cctttgaact attctgaaga agatctatat cgcccctacg
     5521 tcagtgtctt gttcggtaca cattacctag ctcgtaaccg cactttgatg aatggtgacc
     5581 tttatgccac cctcgccgcc tacaatggcg gacccggcaa cgccgccgct tggaaagacc
     5641 tctctggcga agaccaagat ctcttcttgg aaagtgtacg gtttgaggaa acacgtaact
     5701 acatccgcaa tatttacgaa atattcatag tgtataaacg attatatggc acacccataa
     5761 cccagtaagt gcgagcctga ataaaataga gggatgaata gactctttca cagagtctat
     5821 tcatccctct attatttaat caaattcgtt ttattctacg tgcgcaacaa tgcgttgatg
     5881 gacagtttcc acatccatat ctaacacaga gatgagttta tctctgccgc tttcaccagc
     5941 gacaatttcc acacgggtgc gaggcacgcc cagcacctca gcgagaaagg caagtaagtc
     6001 gctgttcaga tttttatcat cggggtcagc agccaactga atcttgacag taccgtcatt
     6061 taagacccca gcgatctggt tacggctcgc acgtggggta atacgtacag ccaaggctga
     6121 accgcgcttg ccatcatgaa gaacatattc tctggacata tcatcacctc acaataggga
     6181 acgtaaaaaa ttagttatta actcaatgat caagatcaaa atcagtggac taaaatcaag
     6241 cccgccaccg ctgggcatca aacttcgaat cgggtttaag aacgggtcaa caatttttcc
     6301 caatgcttcc cgcacagggt gatagggtgg tagaaaaaac gagagcaacg aagatgcaat
     6361 cacaacccat acaaagaagt cagcaacgat gatgatgatg gatattatag tattgccgtt
     6421 cataaattct ccgctacttg atagtttctt ggtccaacaa tggcagtgcc gatccgtacg
     6481 aacgtggcac cttcttccac agcaacttca taatcagaac tcgttcccat tgaaagctct
     6541 atccactttg cttgaggcaa atggctcgta aagaactctt gcaagcgccg cagcttttgg
     6601 aaatacgggc gcgcaaactc tgcgtccatt ccaagcggcg gcatagacat caaaccgcgg
     6661 acttgcaaat tgggcatagc aagcaactca gaaacttcgg gcaataactc ttcccagcgc
     6721 gtttcatcaa cggcttccca accgcccttg ctatcttcgc cgcccacgtt gaactctaat
     6781 aagaccggca gcaccctgcc cgcctcaccg caaaaccgat ccagcttctt cgcaagtttt
     6841 aggctatcca atgaatgcat gaagttaaag tgccctgcca ccagcggcgc ctttcggctc
     6901 tgcacgtgac ctatcatgtg ccattctacc gcagaaagat tttgcaaaga ttgaattttc
     6961 gtgacaccct cttcggcata attttcgccc aagatgaccg cccctgcctc aatcgccgcc
     7021 tgaacgacct ccagcggctg gaatttggtg acgacgacca acttcaccgc ctccggactt
     7081 cgccccgacc tgttcgccgc tgacgcaatt gtatccaacg ccgacagata ccgctctcgt
     7141 atagaattga ccaattcgct catatgatta ttttaaccca cccagggttt tctcaaacca
     7201 ttgccaacaa cacgccaaaa cgacctgttt tccctttttc ggcacggtgt gaataccaat
     7261 cgtctaaatg acaggcagtg cataagcccg atatctgaat atgctccaca cccatcttct
     7321 gcaattgcaa agcatttgcc gcccacagat caagataaat cttctcatcc cgcacctgaa
     7381 caacttgttc agagtctctg ccatatttct cacggaattg cgcagcaact tcctcaccta
     7441 cctcataatg atctgcacta atagacggac caatgccaac cacaatatct tgcggattgg
     7501 aaccgtatcg ttcttgcatg gcttgcacag agacctcagc gacccctttc acagttccca
     7561 tccaacctgc gtgtgaaata ccgattacgt ttttcttcgg atcgtgaaaa agcaaaggga
     7621 cacaatctgc aaagcgcatg aagagcgaaa cttcagggtt gtcggtaaaa aggatatctg
     7681 ctttttgagt cggagcatcg agcgagcgcg gggcatccgc gaagataacg tctgtgccgt
     7741 gaatcagcca tgcgtcgtgg atagaggcgg ggtttcgtcc aagcgcatta aaggcatgga
     7801 ctcggttctc agccacgcgc gcgggatcat ctccaaccga aatgcttaag ttgagagaag
     7861 tccacggttc ggggctaatg ccgccctgac gagtaaacac cgcattgagg atattctttg
     7921 aaaagatatc gaactggtaa taacgcagcc cgtctgattg gtcaaagggc attattcagc
     7981 cgcctcgatc actgctgact ttttgaggaa atactgacgg gtttcgttca tggattcttc
     8041 gatgttggga taagcaaacc aagccgtaaa gagaccaaag agcgcgccgg taagaacacg
     8101 taagaacggt gtactttcac gatatggtag aagtgtattg agccaatccc aattgaactg
     8161 actgaagagt tgcgaaaagc cgtctaaccc gatgggcgca atgccgatca aaatccacac
     8221 cagccaatgt acagacttga agcggcgacc ggtgagtcca aagatgatgc cgaatgccag
     8281 aatagcaaga tagatcgcta catcgcgttc gcaaagcgcc actttgtaac caatggttga
     8341 atcaccaata tagttgcggg cttcgaagcg agccatattg tatggatcat ctacacctgc
     8401 aatgccggtg gcttgctcaa aggtcttata gcctgtgagg ttcgcctctg ccagcggata
     8461 aaaaggctgt tcgccaaaca agaagaaaga acgaaaagca aattgatggc aaagaggttt
     8521 atagatgcga tagatcagtt gcgcgggtgc ttcggcgccc aacttcatga gggttggcgc
     8581 aagaaaaggc agccctacat acaacaacat gaacagattc aacaaaagca aataacgctt
     8641 tgatatccag aatgaaacac ggtctccagt agacaatttc tttcctttct ccattttgta
     8701 tttataaacg gggt
//
LOCUS       DAOQNJ010000037         8656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4048_length_8656_cov_8.621323, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000037 DAOQNJ010000000
VERSION     DAOQNJ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4048_length_8656_cov_8.621323"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLG52_02075"
     CDS             <1..215
                     /locus_tag="PLG52_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40255.1"
                     /translation="SHCVAWTVQDLLDDILAVDPQLAKKVYLLEDCSSAVVVPGVVDH
                     TDAANEAYGRFARAGMHVVKSTDEIA"
     gene            289..1632
                     /locus_tag="PLG52_02080"
     CDS             289..1632
                     /locus_tag="PLG52_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNB40256.1"
                     /translation="MSIFDGKRLTNETFKLDIDRMRRGWYSDKYFENINRMLTALAKE
                     GYTYSGNYHNLPEGMSPEQIAVGDIEVEMQWFTRRMGKTTVVGVDKSLEMLKHCSGYF
                     DGDEFIDTSGNLEVWAVHDGTTVKYDGDPSKIQPVIKVRGRYRDFALLETPTLGILTR
                     SSRVATNVYETLVAAKGKPVLFFPARFDVHEVQAADGYAYNIALQRFNKDYARELGAF
                     VSTDAQGDWWGGAGGGTVAHAAIASFLGDTAEAMMQFSHILPARIPRIALVDFNNDSV
                     RDSLRTLDAMFAKYRELMDAGKTEEAEKYKLYGIRLDTGGNLRDVSVTPLGEPALDLG
                     VNPRLVFNVRSALDSAWERWDLPTAWKEAAKEFCHSVKIVVSGGFAPEKIRKFEKLDV
                     PVDIYAVGSYLFSNGNGTSTDFTADVVRVKVHGEWIDMAKVGRKVGENENLERVW"
     gene            complement(1676..2431)
                     /locus_tag="PLG52_02085"
     CDS             complement(1676..2431)
                     /locus_tag="PLG52_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNB40257.1"
                     /translation="MNKIEIENLSLQYADGAESLRGISMGIRQNAVTVLFGPAGGGKS
                     TLLRCLNRLNDLTDIRTSSGRILIDGENILDPKTDVIALRRKVGMVFARPVPLPMSIR
                     ENITYGMELAGEKRRIKLDEAVERSLKLAAIWDEVKDRLNDPAVALSGGQQQRVCLAR
                     VLALQPEIILLDEPTSGLDPISTGKVEAALQELKKNYTVILVPHSVQQAARTADHAAF
                     FLQGELIEYDEGKRMFTTPRQKKTEDYIMGRFG"
     gene            complement(2443..3306)
                     /gene="pstA"
                     /locus_tag="PLG52_02090"
     CDS             complement(2443..3306)
                     /gene="pstA"
                     /locus_tag="PLG52_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNB40258.1"
                     /translation="MMKMLARNRHTVQRIGYALITLMAVVTVFPIVGTVIFILFKGGS
                     AISWEFLTGFPHDGMRAGGILPAIIGTLYLTIGTAIFSVPLGIAAAIYLSEYAKDNQV
                     TRLIRLAIINLAGIPSVVYGLFGLGLFVLFLQFGTSILAASLTLSIMTLPVIISTAEE
                     ALRSVPQQFRTVSISLGATRWQTIRKIVLKEALPGILTGVILGLERAAGETAPILFTG
                     AAFFLPRLPQSPFDATMALPYHLFVISTQVPEMPIQIQYGTALVLLVFVLTMNVIATI
                     IRSRARAKRQW"
     gene            complement(3303..4199)
                     /gene="pstC"
                     /locus_tag="PLG52_02095"
     CDS             complement(3303..4199)
                     /gene="pstC"
                     /locus_tag="PLG52_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNB40259.1"
                     /translation="MEKSLNWREFIITRAIKASGYSAIVFVLLIFFFLMREGLPALSE
                     VETSALFSTRWYPIENYFGILPLITGSLIVTLGAALIAVPFGIGTAIYISEIAPRWMR
                     EILKPLVELLGGLPSVVLGFLGILVVSPNLRILLDLPTGLTAFTGSLLLGGIAVPTVV
                     SVAEDALDAVPRSYREGAWALGATPWQTIWRVTLPAAKSGVLTAIMLGVGRAIGETMT
                     VMMVTGNAPVLALKLGSLFSPVRTMTATIAAEMGEVANGSVHYHTLFLIGIVLFLISL
                     IVNVVASSVVFRAKKRAERILS"
     gene            complement(4251..5123)
                     /locus_tag="PLG52_02100"
     CDS             complement(4251..5123)
                     /locus_tag="PLG52_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB40260.1"
                     /translation="MKRVIVFFLLLSILLISCGSSSTDTSSDSPAQYIENKGSDTIVN
                     LALAWAETYQAEFPDVRISVTGGGSGTGIAALINGTVDIANASRKIKDEELTEAQSKG
                     VDPLEHIIARDAIAVIVNPENPVNELTLQQIADIYSGKITNWREVGGEDRPIVKLSRE
                     TNSGTHVYFLETVLRLGDKENKTLFSTDTLLLPSSEGIIAEVRQNPNAIGYDGLGYVP
                     DDLKMIAIAKETGGTYVLPAIATVNDKTYPIARDLYMYTNGEPTGAVKEYLDWILSPE
                     AQEIVAKLGFVPVK"
     gene            5240..6001
                     /gene="pstB"
                     /locus_tag="PLG52_02105"
     CDS             5240..6001
                     /gene="pstB"
                     /locus_tag="PLG52_02105"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNB40261.1"
                     /translation="MSAPQPAFTVQNMDFYYNTSKKTLSNINLEIQPQKVTALIGPSG
                     CGKSTFLRCLNRMNDTIPGTKVDGKIFLNGEDIYGETMDVVTLRQRVGMVFQKPNPFP
                     QSIFDNVAFGPRVMGMDVKLSEVVEKSLRAAALWDEVKDDLKSDAMGLSLGQQQRLCI
                     ARVIAVQPEVILMDESTSALDPIATLRVEELIAELKKQYTIVIVTHNMQQAARVSDYT
                     GFFWLGEMVEFAQTNQMFTVPKQELTEAYITGRMG"
     gene            complement(6100..6789)
                     /gene="phoU"
                     /locus_tag="PLG52_02110"
     CDS             complement(6100..6789)
                     /gene="phoU"
                     /locus_tag="PLG52_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNB40262.1"
                     /translation="MIRKTFESDIQQVKDEVLALGSMVEHAIISSVEALKKRDIKAAE
                     KIIAEDKEINKKRFAIENQLMILIATQQPMAHDLRLLASTMEIISELERMGDYAKGIA
                     NINVRMADEKLLKPLIDIPRMAEKGASMLHRALTAFVHEDIETAKSLPIEDDEVDALY
                     NQIYRELMIFVIEDPKNIERANWLLWVAHNLERVADRVTNICERTVFIATGEFGEIKS
                     SDDEFWKNQNK"
     gene            complement(6935..7705)
                     /gene="pstB"
                     /locus_tag="PLG52_02115"
     CDS             complement(6935..7705)
                     /gene="pstB"
                     /locus_tag="PLG52_02115"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017789372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNB40263.1"
                     /translation="MENNYALETNDLSIHYGTFTAVKGVNIKVERQKITAIIGPSGCG
                     KSTLLRSFNRMNDFVPGCNVTGELLLQGQNLYGKNIDPVEVRRKIGMVFQKPNPFPKS
                     IYENIAWGARINGFKGNMDELVESSLRSAALWDEVKDKLQQSAYALSGGQQQRLCIAR
                     TIAIQPEIILMDEPASALDPISTLRIEELMQELKKNYTILIVTHNMQQAARASDYTAM
                     MMIDDQRAGGVIEFDDTKNIFTRPKDKRTEDYVTGRFG"
     gene            complement(7718..>8656)
                     /gene="pstA"
                     /locus_tag="PLG52_02120"
     CDS             complement(7718..>8656)
                     /gene="pstA"
                     /locus_tag="PLG52_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNB40264.1"
                     /translation="EEKMEERTQADLLSLFLERLVRIDTKQTWDLAESLTRGAEIRAE
                     VADKYPDAKLEFRSWLTPQFLVSPMSSRAEFAGVRTAILGSLWLVGIAIAFALPIGTG
                     AAIYLEEYATKNWFTRIIQTNINNLAGVPSIVYGMLGLALFVRVMEPFTSGSIFGVTD
                     SNGRTILAAGLTMGVLVLPVIIINAQEAIKAVPDSLRQAAYGVGATRWQTVWHHVLPN
                     ALPGILTGSILALSRALGETAPLIVVGASTFITLDPDSPFSKFTALPIQIYQWTTRAQ
                     SEFHSIAAAAIIVLLVLLLTLNATAILLRNRFQKRW"
BASE COUNT         2075 a         1927 c         2464 g         2190 t
ORIGIN      
        1 agagtcactg tgtggcgtgg acggtgcaag acttgctgga tgacatcctt gccgttgacc
       61 cgcaacttgc caagaaggtc tatctgctcg aagactgttc gtctgcggtg gtggtgcctg
      121 gggtggtgga ccataccgat gccgcgaatg aagcgtatgg tcgctttgca cgagcaggca
      181 tgcatgtggt caaatccacg gatgagattg cttaattgtg taaaacgtag tgcatgatcc
      241 ataatttatg aatcatgcac tacgcattat ttattaaaaa gaggaaccat gtccattttt
      301 gacggtaaac gactgacgaa cgagactttt aaattagata ttgatcgtat gaggcgtggt
      361 tggtattctg ataagtactt tgaaaatatc aatcgtatgc taacagcatt agcgaaagag
      421 ggctatacct attcaggtaa ttatcacaac ttgcctgaag gcatgtcgcc ggagcaaatt
      481 gcagtaggtg atattgaagt ggaaatgcag tggtttacac gccgcatggg caagactact
      541 gtggtgggcg tggacaagtc actggagatg ttgaaacatt gctctggcta ttttgatggc
      601 gatgaattta ttgatacatc aggcaatctt gaggtgtggg ctgtacatga tgggacgacg
      661 gtcaagtacg atggtgaccc gagcaagatc cagccggtca tcaaagtgcg tggacggtat
      721 cgtgactttg ctttacttga aaccccgacc ttgggtattc tcactcgttc aagtcgtgta
      781 gccacgaatg tctatgaaac cttggtcgct gcaaaaggca agccggtttt gttcttcccg
      841 gcgcgatttg atgtgcatga agtgcaagct gccgatgggt atgcatataa catcgcatta
      901 cagcgtttca ataaagatta tgcgcgtgag ttgggggcgt ttgtttctac agatgcgcag
      961 ggtgattggt ggggcggcgc aggcggtggg acggtggctc atgctgcgat tgcatccttc
     1021 cttggcgata cagctgaggc aatgatgcaa ttctcgcata ttctgcctgc acgcattccg
     1081 cgcattgctt tggtggattt caacaatgac tcggtccgcg attcgttgcg gacgttggat
     1141 gcgatgtttg ccaagtatcg tgagttgatg gatgctggga aaaccgaaga agctgagaaa
     1201 tataaacttt acggcattcg tttggacacg ggtgggaact tgcgcgatgt atcggtcact
     1261 ccgttgggtg agccagcatt ggacttggga gtcaacccgc gtttggtatt caacgtccgt
     1321 tcggcgttgg attcagcttg ggaacgttgg gatctgccta cggcttggaa agaagcggcg
     1381 aaggaatttt gtcacagtgt gaagatcgtc gtctcgggtg gctttgctcc agaaaaaatc
     1441 cgcaagttcg agaaactgga tgtgcctgta gatatttatg cagtgggttc atatctgttt
     1501 agcaatggta atggtaccag tacagacttc accgccgatg tggtgcgagt aaaggtccat
     1561 ggcgagtgga tcgatatggc gaaggtggga cgcaaggtag gggagaacga gaatttggaa
     1621 cgggtttggt agttataaaa agtgacagtc accttcaagg tgactgtcac tttatttaac
     1681 caaaccttcc catgatgtag tcttcagttt tcttttgtct gggggttgtg aacattcttt
     1741 tcccttcatc gtattcgatg agttcccctt gtaaaaagaa ggcggcgtga tcggcagtgc
     1801 gggcggcttg ttgcacagag tgggggacga gaatgacggt gtaattcttt ttcagttctt
     1861 gtaatgcggc ttcaaccttg ccagttgaga tcgggtcaag acccgaggtg ggttcatcga
     1921 gcaagataat ttcaggttgc agtgccagaa cacgtgcaag gcaaacgcgt tgttgctgcc
     1981 cgccagagag tgcaacggca gggtcgttga ggcggtcttt gacttcatcc caaatggcgg
     2041 caagtttcaa actgcgttcc actgcttcgt cgagtttgat gcggcgtttt tctccggcaa
     2101 gttccatacc ataggtgatg ttctcacgaa tgctcatggg gagcggcaca ggtcttgcaa
     2161 acaccatgcc tactttgcgg cgtaaggcga tcacatcagt tttggggtcg aggatatttt
     2221 cgccatcaat gaggatgcga ccagaactgg tgcggatgtc ggtcagatcg ttgagacgat
     2281 tgagacatcg caacaaggtg gatttcccgc ctcctgccgg accgaagaga acggtcactg
     2341 cattctgacg gattcccatg ctgatgccgc gcaatgactc tgcgccatcg gcgtattgga
     2401 gggagaggtt ttcgatttcg attttgttca tttgacgatt gattaccact gacgttttgc
     2461 tcttgcccgc gaacgaatga tggttgcgat cacgttcatg gttaagacaa acacgagcaa
     2521 gaccaacgcc gttccatatt ggatttggat tggcatttca ggcacttggg tggaaatgac
     2581 aaagagatga taaggcaatg ccatggtcgc atcgaaagga gattggggca agcgcgggag
     2641 gaagaacgcg gcacccgtga agaggatggg tgcagtctca ccggcggcac gttccaagcc
     2701 gagaatgact cccgtgagga tgccgggtaa tgcttctttc aatacgatct tgcgaatggt
     2761 ttgccaacga gtggcgccaa gggagatgct taccgtgcgg aattgttgcg gcaccgagcg
     2821 caacgcttct tctgcggtgc tgatgatgac gggcaaggtc atgatcgaga gagtcaatga
     2881 ggcggcgagg atggaggtgc cgaactgaag aaagagcacg aacaagccca agccgaacaa
     2941 tccatacacc accgaaggga tgcctgccag gttgatgatc gccaaacgaa taaggcgcgt
     3001 gacttgatta tccttggcgt attcagaaag ataaatggcg gctgcgatcc caagcggaac
     3061 ggagaagatg gcagtgccga tggtcagata gagtgtgccg atgatggcag gcaaaatacc
     3121 accagcgcgc atgccatcgt gtggaaaacc ggtgagaaat tcccacgaga tcgcagagcc
     3181 gcctttgaat aagatgaaga taaccgtgcc cacaatggga aaaacagtca ccactgccat
     3241 aagggtgatg agagcgtagc caatgcgctg cacggtgtga cgatttcgag cgagcatttt
     3301 catcatgaca aaattctctc cgctcgtttc ttggcacgaa agacgactga ggatgcgaca
     3361 acattcacga tcaatgaaat aaggaacaag acgatgccaa tcaagaacaa ggtgtgatag
     3421 tggacgcttc cattcgcaac ttcgcccatc tctgcggcaa tggtggcggt catggtgcgg
     3481 acgggggaga agaggctgcc gagtttgagt gccaatacgg gcgcattgcc ggtgaccatc
     3541 atcacggtca tggtttcgcc gatggcgcga cccactccca acatgatggc ggtcagcacg
     3601 ccggattttg ctgcgggaag cgtcacacgc cagatggtct gccagggagt ggcacccaaa
     3661 gcccatgcgc cttcacgata agaacgcggc acggcatcga gtgcatcttc tgcaacagac
     3721 acgaccgtgg gtacggcgat cccgccgagg aggagggatc cggtaaaggc ggttagaccc
     3781 gttggcaggt ccagcaagat gcgaaggttg ggggaaacaa cgaggatgcc gagaaatccc
     3841 agcactaccg aagggagtcc gccgagcaat tccacgagag gtttgagaat ttctcgcatc
     3901 cagcgtggcg caatttcaga aatgtagatg gcagttccga ttccgaaagg cacggcgata
     3961 agcgctgcgc caagggtgac gatgagagaa ccggtgatga gtggcagaat accgaaataa
     4021 ttttcaatgg gataccaacg tgtgctaaag agtgcgctgg tttccacttc gctcaaggcg
     4081 ggcaagcctt cacgcatgag aaagaaaaag ataagcaata caaatacgat ggcggaatat
     4141 ccgctcgctt tgatggctcg ggtgatgata aattctcgcc agtttaaaga tttttccata
     4201 gtgctccatc gtcattgcga gggtgaagat catcgcaatg acgaaatgat ttatttcaca
     4261 ggcacaaacc caagttttgc cacaatctct tgcgcttcag gggagaggat ccaatcgaga
     4321 tattctttca cagcgccagt gggttcacca ttggtataca tataaaggtc acgcgcaatg
     4381 ggataggttt tgtcgttcac ggtagcaatg gcaggtaaga cataagtgcc gcctgtttct
     4441 tttgcaattg cgatcatctt gaggtcatct ggaacatagc ccaagccatc gtacccaatc
     4501 gcgttcgggt tttgccttac ttcggcaatg atgccttctg aagacggaag tagcaaggtg
     4561 tctgttgaga acaacgtttt gttttctttg tctcccaatc ttaagacggt ttccaaaaag
     4621 taaacatggg tgcctgagtt ggtttcgcgt gaaagtttta cgataggtcg atcttcgccg
     4681 ccaacttcac gccagttggt gatcttgccg ctgtaaatat ctgcgatctg ctgcaacgtc
     4741 aattcgttga cggggttttc tgggttcacg atcacggcaa tcgcatcgcg tgcgatgatg
     4801 tgctcaagcg ggtccacccc tttggattgg gcttcggtca actcttcatc tttgattttg
     4861 cgtgaagcgt tggcaatatc cacggttccg ttgatgagcg cggcaatgcc tgtgcctgaa
     4921 ccaccgcctg tcaccgagat gcgcacatcg gggaattcgg cttgataagt ttctgcccat
     4981 gccaacgcca gattgacgat ggtatcggag cctttgtttt cgatgtattg cgcgggggag
     5041 tcagagcttg tgtcagtgga tgaagaacca caagagatta atagaatgga aagaagaagg
     5101 aagaagacga ttacgcgttt catcggctgg gattataccg cctcaaactt gacggaggct
     5161 tgattctgac cctgacgctt tttgccactt tatgaatttt ttttaccgct ttgattttcc
     5221 tttttgatac aatcaaatca tgagcgcacc ccagcctgcc tttaccgtcc agaacatgga
     5281 cttttactat aacacatcaa aaaagaccct ctcgaacatc aacctggaga ttcagcccca
     5341 aaaggtgacg gcgttgatcg gtccatcggg ttgtggaaaa tctacgttct tgcgttgtct
     5401 taaccgcatg aatgacacca ttcccggcac caaagtggat gggaagatct ttctcaatgg
     5461 tgaagatatt tatggcgaaa cgatggatgt ggtgacactc cgtcagcggg tggggatggt
     5521 cttccagaag ccgaacccat ttccgcaatc cattttcgat aatgtggcgt tcggtccgcg
     5581 ggtgatgggg atggatgtga agctcagcga agttgtggaa aaatctctgc gggcggcggc
     5641 actttgggac gaagtaaaag atgatctgaa atcagacgcg atgggcttgt cattggggca
     5701 gcaacaacgc ctgtgcattg cacgcgtcat tgctgtgcaa ccagaagtga ttctgatgga
     5761 tgaatcgact tcggcattgg acccgattgc cacactgcgc gtggaagaac tgatcgcaga
     5821 attaaagaag cagtacacga tcgtgatcgt gacccataac atgcaacaag ctgcgcgtgt
     5881 ttctgattac accggctttt tctggttagg agagatggtt gagtttgcgc agaccaatca
     5941 gatgtttacg gtgccaaagc aagaattgac agaagcgtat attaccggaa gaatgggcta
     6001 attagtctgg tagtttgtta gtcgaatagc cttgtagtta ttaaacaaag cgagtggatg
     6061 agttttctca tccactcgct ttttatttct tgtaacgtat tacttattct gattcttcca
     6121 gaactcatcg tcgctggatt tgatctcgcc gaattcaccg gtggcgatga agacggtgcg
     6181 ctcgcagatg ttggtaacac ggtctgcaac gcgctcgagg ttgtgggcaa cccacaacag
     6241 ccagttggcg cgttcgatgt tcttggggtc ttcgatcacg aagatcatca actcacgata
     6301 gatctggtta tagagtgcgt ccacttcgtc atcttcgatg gggagggact tggcagtctc
     6361 tatatcttca tgcacaaagg cggttagggc gcggtgcagc atgctggcgc ctttctctgc
     6421 catacgtgga atgtcgatca gcggcttgag caatttctca tctgccatgc ggacgttgat
     6481 gttggcaatg cctttggcgt aatcgcccat gcgttccagc tcagagatga tctccatggt
     6541 ggaggcgagc aggcgcaggt cgtgcgccat gggttgttgg gtggcaatga ggatcatcag
     6601 ctggttttcg atggcgaaac gttttttgtt gatctcttta tcttcggcaa taattttttc
     6661 agcggctttg atatcgcgct tttttaacgc ttccactgag ctgatgatgg catgttccac
     6721 cattgagccg agtgccagca cttcatcttt tacctgttga atgtcgctct caaaagtttt
     6781 acggatcata agtcctcttt aggttaattg tgttcatttt actacttttg cgattatcga
     6841 ttctatctga cggtgccgtt tcggaagggt ccgctcaagg ggagagaaag gtttgggcgg
     6901 gcgaacaggc aaaacgtctg aaagaggcgg ctttttatcc aaatcttccg gtgacgtaat
     6961 cctcggtccg cttgtctttg gggcgggtga agatgttctt ggtatcgtca aattcgatca
     7021 caccgccggc acgttggtcg tcgatcatca tcatggctgt atagtcactg gcgcgcgcgg
     7081 cttgttgcat gttgtgagtg acgatgagaa tggtgtagtt ctttttcaac tcttgcatca
     7141 actcttcgat gcgcagcgtg gatatcggat ccagcgcaga ggcaggttcg tccatcaaaa
     7201 tgatttcggg ttgaatggcg atggtgcgtg caatgcagag acgttgctgc tgaccacccg
     7261 agagggcata ggcgctttgt tgaagtttgt cttttacttc atcccaaagg gcggcggaac
     7321 gtagagagct ttcaacgagt tcgtccatat tgcccttgaa tccattgatg cgagcgcccc
     7381 aagcgatgtt ctcgtaaatg gatttgggaa aggggttcgg tttttgaaag accatgccga
     7441 tcttgcggcg cacttccacg gggtctatat ttttgccgta gagattttgt ccttgtaaaa
     7501 gcaattcacc tgtgacgttg caacccggca cgaagtcatt catgcggttg aaagaacgta
     7561 atagcgtgga tttgccacag ccagacggac cgatgatggc agtgatcttt tggcgttcca
     7621 ctttgatgtt cacgcctttg acggcagtga atgttccata atgaatggat agatcgttag
     7681 tttcgagtgc gtaattattt tccatgattg agttccgtta ccagcgtttc tggaagcgat
     7741 tgcgaagcag aatggcggtt gcgttgagag ttaagagcaa caccagcagg acgatgatgg
     7801 cagccgcggc aattgaatgg aattcagatt gcgcacgagt tgtccattga tagatttgga
     7861 tgggcagcgc cgtaaatttt gagaagggac tatccgggtc gagcgtgatg aaggtggagg
     7921 cgcccaccac gatcaacggg gcagtttcac ccaaggcacg cgataatgcc aggatgctgc
     7981 ccgtgaggat gcccggcaat gcgttcggca aaacatgatg ccaaacggtt tgccagcgcg
     8041 tcgcgcccac gccgtaggcg gcttgccgca gtgaatctgg gacggctttg atagcttctt
     8101 gtgcgttgat gatgatgacc ggcaacacca gcacgcccat cgtcaacccg gcggcgagga
     8161 tggtgcgccc gttcgagtcg gttacgccga agatcgagcc gctggtgaaa ggctccatta
     8221 cacgcacgaa taaggcgagc cccagcattc catatacaat gctgggaacc ccggcaaggt
     8281 tattaatatt ggtttgaata atgcgagtga accaattctt cgtggcgtat tcttcaaggt
     8341 aaatagcggc acccgtgccg atgggtaagg cgaaggcgat ggcaataccg accaaccaaa
     8401 gcgaaccaag aatggcagta cgcaccccgg caaactctgc ccgcgaagac atgggcgaga
     8461 cgaggaattg tggtgtgagc cacgagcgga attccagttt ggcgtcggga tatttatcgg
     8521 caacttcggc gcggatctct gctccacggg tgagggattc ggcgaggtcc caagtctgtt
     8581 tggtgtcgat gcggacgagg cgttcaagga agaggctaag caaatctgct tgagtgcgtt
     8641 cttccatttt ttcctc
//
LOCUS       DAOQNJ010000038         8522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4159_length_8522_cov_6.551435, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000038 DAOQNJ010000000
VERSION     DAOQNJ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4159_length_8522_cov_6.551435"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /gene="kdpB"
                     /locus_tag="PLG52_02125"
     CDS             complement(<1..1858)
                     /gene="kdpB"
                     /locus_tag="PLG52_02125"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNB40265.1"
                     /translation="MTTKKTARHEMYQRAVLESFIKLNPCWMVRNPVMFVVEVGSVLT
                     TLLWIQSLIGKGEAPAGFIAAIALWLWFTVLFANFSEAVAEGRGKAQAESLRKARQDT
                     PAKKVSSKVEKFESSNVEHEIVSSTSLRKDDLYLVVAGDILPADGEIVVGIASVDESA
                     VTGESAPVVREAGGDRSAVTGGTRLLSDWLIIRVSADPGQGFLDRMISMVEGAKRQKT
                     PNEIALNILLAGFTIIFLVVCATLLPYSIYSVEASGSGTPITITVLIALLVCLIPTTI
                     GGLLSAIGIAGMDRMIQRNVIAMSGRAVEAAGDVDVLLLDKTGTITLGNRQAVEFIPV
                     NGANANELAEAAQLASLADETPEGRSIVVLAKEKFGLRGHTVDGMHFIPFTAQTRMSG
                     VDFNGTQIRKGAPDTMIGLIQSNGNGVASDLKPTVDSIARAGGTPLVVTRDNKALGVI
                     HLKDIVKGGMKERFAQLRKMGIKTVMITGDNPLTAAAIAAEAGVDDFLAQATPEAKLK
                     LIREYQTGGRLVAMTGDGTNDAPALAQADVAVAMNTGTQPAREAANMVDLDSNPTKLI
                     EIVEIGKQLLMTRGALTTFSLANDVSKYFAIIPAAFASTYPQLNALNIMRLAT"
     gene            complement(1907..3622)
                     /gene="kdpA"
                     /locus_tag="PLG52_02130"
     CDS             complement(1907..3622)
                     /gene="kdpA"
                     /locus_tag="PLG52_02130"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNB40266.1"
                     /translation="MNTWLQLILYFGILLLLVKPLGAYMAKVYQGERLFLDPILGHVE
                     RFLYRLSGIDQRSEMTWKTYAVAMLIFNILGVITVYLLQRLQETLPLNPQGLGAVSPD
                     SAWNTAVSFASNTNWQGYGGETTMSYLTQMLGMTVQNFVSAATGMAVMVALIRGIVRH
                     TTKEIGNFWVDMTRSVLYILLPLSLVLSLALVSQGVVQTFSEYKSVTSLQSGETQLIA
                     VGPAASQVAIKQLGTNGGGFFNANSAHPLENPTPFSNFLEMLSILLIPAALCYTYGKM
                     VGDTRQGWALLAVMTIVLVALLGVAIWSEQSGNPLFAKLGIDQTQSATNPGGNMEGKE
                     VRFGVVNSALWATVTTAASNGSVNSMHDSFMPLGGLVPMWLMQLGEIIYGGVGSGLYG
                     MLAFVIIAVFVSGLMVGRTPEYLGKKIEAYEMKMASIIILIPVMVVLLGTAVALMTEA
                     GRATIFNPSAHGFSEVLYAFSSAGNNNGSAFAGLGANTPFYNIALGIAMFIARYWLAI
                     PVLAIAGSLANKKKVPASAGTLPTHTPLFIAWVIGVIIIVGALSFLPALALGPIVEHL
                     MVLGK"
     gene            complement(3619..3711)
                     /gene="kdpF"
                     /locus_tag="PLG52_02135"
     CDS             complement(3619..3711)
                     /gene="kdpF"
                     /locus_tag="PLG52_02135"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HNB40267.1"
                     /translation="MNPLYWIAGIVALGLLLYLVFALVKPEWFG"
     gene            complement(3888..>5227)
                     /locus_tag="PLG52_02140"
     CDS             complement(3888..>5227)
                     /locus_tag="PLG52_02140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB40268.1"
                     /translation="QKNAMPQKPSENMLKAIAHGGFMSHYDEPFDIGVTASFGDRSKR
                     TLSQLFVPVISGSRTIGVLSIQSYQKYFYKDEQLELLKDLASHCAGALERIWAQEAVN
                     DLVERLKVLHAAVNAINASLEMERVCQVVYETVKQVMPCDDFVIDGYDKDTNEIVPIY
                     AIEYPGKRVFTNRYLADHGMAGEIVLSKKSLLFNSVEEMDNSGIKFELYGTHEDDPSQ
                     SILAVPMTLHGDMYGMVSAQSYNTNAYNRDHLYLLEVLASHAAIAIENARLFDSIQNL
                     ANTDSLTKILNRRRFFELAEQEFVRAEQFNIPFSVIMIDVDDFKQFNDKHGHKIGDAV
                     LILAAETLKSSLRTDDILGRIGGEEFVAALPNTPIPFAMEVASRLRQAIQDTNFSERI
                     RHEMPDLLITVSIGVAEYASNCKTLDTLLDRADQAMYNSKNSGRNQVRAWDAS"
     assembly_gap    5228..5327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5328..5614)
                     /locus_tag="PLG52_02145"
     CDS             complement(<5328..5614)
                     /locus_tag="PLG52_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40269.1"
                     /translation="MSSKPARQNSRSETAVFANLGRDLASTTEARSAAEIILNAADSL
                     IGWDAAYLILYDPAIGGVPRPLLTIDTINGNHVEQKNAMPQKPSENMLKAIA"
     gene            complement(5611..6384)
                     /locus_tag="PLG52_02150"
     CDS             complement(5611..6384)
                     /locus_tag="PLG52_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB40270.1"
                     /translation="MDITSLQNPRVKHIVKLREDKKQRQKDRLILVEGYDELTLALSC
                     GFEPQTLLSAPELVSQSLTVPNAENLTVSRAVFEKISYRDNPDGWLGLFPMPTSSLDE
                     LKLSENPLVIVAESVEKPGNLGAILRTADAAHVDAVLVCDPRVDTWNPNTIRASRGAV
                     FAVPVVEVKSAEALSWLRSRKMRVLAATPSADAMYTDVDMQGPLAIAVGTEDEGLTDF
                     WMQNSDLRVKIPMTGKVNSLNVSIATALMTYEAVRQRAK"
     gene            complement(6385..7383)
                     /locus_tag="PLG52_02155"
     CDS             complement(6385..7383)
                     /locus_tag="PLG52_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40271.1"
                     /translation="MKNTTEKLLEIRGLKKYFEAGRPSLFSRETKHVHAVDGVNLTLR
                     RSEVIALVGESGCGKSTLGLLLMGLEDPTDGSVIFEYRDITHLNDHQRKDLRRKIQMV
                     FQDPYESLNPTQTIEEIVTEPLTVHGMASSHQDRDERVRKAMEDAGLKPADVYLHRYP
                     HELSGGQRQRVVIASALVLEPEIIIADEPVSMLDVSIRAEVINLLAELRISRQIAVIF
                     ITHDLGSVGFFADRVAVMYLGRIVEIGTMLEVLEKPQHPYTKALLSVIPVPNPRLRHE
                     RIVLQGETPNPINIPSGCRFHPRCPAAFDACKLSDPPMTTLSKSHQAACLLVTNTK"
     gene            complement(7380..>8522)
                     /locus_tag="PLG52_02160"
     CDS             complement(7380..>8522)
                     /locus_tag="PLG52_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB40272.1"
                     /translation="GAWWALFAPGFGIVWVVLSLTLLGQGLEQVLNPRLDTHHLMPGK
                     PVVQNEAGAKPIQQDALLEVQNLSINYVTEGKAPARAVENVSFTLKAGELIGLVGESG
                     CGKTTLMLALLKLLPSAGQIVNGKVFYSGQDITAMNEDEINTVRWSGVSIVFQGAMNA
                     LNPVRTVGDQIGEAILKHEPSFPKDKINDRVSQLLELVGIASAHRDHFPHQYSGGMRQ
                     RAMIAMALACNPKVIIADEPTTALDVMIQAQILELLDSLRKKLGLAVIFVTHDLGVVA
                     ELCDKVLVMYGGVTAEYADVDVIYNSPQHPYTQELLKAFPDLTKPKKKLSSIPGYPPR
                     LDNLPQGCRFAPRCPAAFDRCHTEEPLLHTIANGHVVSCHLIEKSK"
BASE COUNT         2134 a         2136 c         2109 g         2043 t
ORIGIN      
        1 gcgtagcaag gcgcataatg ttgagtgcgt tcagttgcgg gtatgtgctt gcaaacgcgg
       61 cagggatgat ggcaaagtat ttgctcacgt cgttggcaag gctgaaagta gtcaacgcac
      121 cgcgagtcat cagcagttgt ttgccgatct ccacgatttc gattaacttc gtggggttgc
      181 tgtccaagtc caccatgttg gcggcttcac gcgcgggctg tgtgcctgtg ttcatcgcca
      241 ctgcaacgtc agcttgagcg agggcgggag catcgttggt gccatcacct gtcatcgcga
      301 ccaagcgtcc gcccgtttga tattcacgga tgagtttgag tttggcttcg ggtgtggctt
      361 gcgccagaaa atcgtccacg cctgcttcgg cggcgatggc ggcagccgtg agcggattgt
      421 cgcccgtgat catcacggtc ttgatgccca tcttgcgcag ttgcgcaaag cgttccttca
      481 tgccgccttt gacgatgtcc ttcaagtgga tgactcccag cgccttgttg tcgcgagtca
      541 ctaccagcgg agtcccgcct gctcgggcaa tggaatcaac tgtcggcttg aggtctgagg
      601 cgacgccgtt tccgttcgat tgaatcaatc cgatcatcgt gtccggcgcg cctttgcgga
      661 tttgcgtccc attgaaatcg acaccactca tacgtgtttg cgcggtgaat gggatgaagt
      721 gcataccgtc tacagtgtga ccacgcagac caaatttttc tttggcaagc accacaatgg
      781 aacgaccttc aggagtctca tcggcaagcg acgcaagttg tgcggcttcg gcgagttcgt
      841 tggcattggc gccattgacg gggatgaact cgaccgcctg acgattgccg agcgtgatgg
      901 tgcccgtttt gtcgagcagc agcacatcca catcgcccgc cgcttcaact gcgcgacctg
      961 acatggcgat gacgttgcgc tgaatcattc tgtccatacc tgcgatgccg atggcagaca
     1021 gcagaccgcc gatggtagtg gggatgagac acacgagcag cgcgatgagc accgtgatgg
     1081 tgatgggtgt gccgcttcca ctggcttcga cgctgtaaat ggaatacggt agcagcgtgg
     1141 cgcagacgac gagaaagatg attgtgaagc ccgccagcaa aatattgagc gcaatctcgt
     1201 tcggcgtctt ctgacgtttc gcgccttcga ccatgctgat catgcggtcg agaaatccct
     1261 gcccagggtc ggcgctgacg cggatgatga gccaatcgga aagaaggcgc gtgccgccag
     1321 tgaccgcgga acggtcaccg cccgcctcac gcacgacggg agctgattcg cctgtcacgg
     1381 cgctttcatc caccgaagcg attccaacga cgatctcgcc gtcagcaggg agaatatctc
     1441 cagccacgac taaataaaga tcatcttttc tgagtgaagt ggatgaaaca atttcatgct
     1501 caacattcga actttcaaac ttttcaacct tcgaactgac tttctttgca ggtgtatctt
     1561 gccttgcctt acgcaacgac tcggcttggg cttttccacg tccttcggcg acagcttcag
     1621 agaagttggc aaataagacc gtgaaccaca gccaaagcgc aatagcagca atgaaaccag
     1681 caggcgcttc acctttgccg atcagggatt gaatccacaa cagggtggtg agcacgctgc
     1741 ccacttccac cacgaacatg acagggttgc gcaccatcca acacgggttg agtttgataa
     1801 aggattccag cacggcgcgt tgatacattt cgtggcgggc ggttttttta gtcgtcattt
     1861 tttatttcct caggagtggt aaagggtaag tggtaattac catttactat ttaccaagca
     1921 ccatcaagtg ttccacaatc ggtcccaatg ctaaggcagg caggaaactc aacgcaccga
     1981 cgataatgat gacaccgata acccaggcaa tgaaaagcgg agtgtgcgtg ggcaaggtcc
     2041 cggcactggc tggaactttc tttttatttg ccagtgaacc tgcaattgcc agtacaggaa
     2101 ttgcaagcca gtagcgagca atgaacatgg caatgccgag cgcgatgtta tagaatggcg
     2161 tgttcgcgcc cagcccagca aatgctgagc cgttgttatt tccagcggac gaaaaggcat
     2221 acagcacttc actaaaccca tgcgcagacg ggttgaagat cgtggcgcgt cctgcttcag
     2281 tcatcaacgc tacagcggtg ccaagcaaaa caaccatgac ggggatgagg atgatgatgg
     2341 acgccatctt catctcgtag gcttcgatct tctttccgag atattcaggt gtgcgtccca
     2401 ccatcaagcc ggagacaaat accgcaatga tgacaaatgc caacatgccg tataagcctg
     2461 accccacgcc gccgtagatg atctcgccaa gttgcatgag ccacatcggg acgagtccgc
     2521 ccagcggcat gaacgagtca tgcatcgaat tcaccgaacc gttcgacgcg gcagtcgtca
     2581 ccgttgccca cagcgccgag ttgacaaccc cgaagcgcac ttctttgcct tccatgtttc
     2641 cgcctgggtt ggtcgcagac tgtgtctggt caatgccaag ttttgcgaac agcggattac
     2701 cactttgttc agaccaaatt gcgacaccaa gcaacgcaac cagcacaatc gtcatcactg
     2761 ccagcagcgc ccaaccttga cgggtatctc cgaccatctt gccgtaggtg taacaaagcg
     2821 cggcaggaat taatagaatg gacaacatct ccagaaagtt tgagaacgga gttgggtttt
     2881 ccaatggatg cgccgagttg gcattgaaga atccgccgcc gttggtgcca agttgtttga
     2941 tggcaacctg cgaagcggca ggtcccaccg cgatgagttg ggtctcacct gattgaagcg
     3001 aagtgacgga tttatattcg ctaaaggtct gcacgacgcc ctgcgaaacc aaagccaatg
     3061 acaatacgag agaaagcggc agcaggatat agagaaccga gcgggtcatg tccacccaga
     3121 aattaccgat ctctttggtg gtgtgacgga caatgccgcg aatcaacgcc accatcactg
     3181 ccatacccgt cgccgcagag acaaagttct gcacggtcat gccgagcatt tgggtgagat
     3241 aactcatcgt cgtctcgccg ccgtagccct gccagttggt attgctggca aagctcactg
     3301 ccgtattcca cgcggagtct ggcgagactg cgccgagtcc ctgcgggttg aggggcagag
     3361 tctcttgcag gcgttgcagc aagtaaacgg tgatcacgcc gaggatgttg aagatcaaca
     3421 tggcgacggc gtacgttttc cacgtcatct cggaacgctg gtcaatgcct gagaggcgat
     3481 aaaggaatct ctcaacgtga cccagtatcg ggtcaagaaa gaggcgctcg ccttgataga
     3541 ctttcgccat gtatgcgcca agtggcttca caagcaggag caaaatccca aagtaaagaa
     3601 tcaattgcag ccaggtgttc atccaaacca ctccggtttg accagtgcga agacgagata
     3661 tagcaaaagt cccaacgcca cgattccagc gatccaataa agcgggttca tgacttgtcc
     3721 tcctgcaacc catcgagcgc gtaaagcagc ccgagagaaa gcaaaaagaa cagaacggtt
     3781 attcctagaa acaaaaggtc gttcatgcgt gcctccatat ggaagcagtg tattcagttg
     3841 taggtcaatt tggggttaag gagagagaaa agggtatcaa gaaaaaatca agaagcatcc
     3901 caagcgcgga cttggtttcg ccctgaattt ttcgagttgt acattgcttg gtcggctcgg
     3961 tctagaaggg tgtcgagagt tttgcagttg cttgcatatt ctgcaacgcc tatactaacc
     4021 gtgattaata agtctggcat ttcgtgccga atacgctctg aaaaatttgt gtcttgaatc
     4081 gcttgccgca aacgagacgc aacctccatc gcaaagggga ttggtgtatt gggaagagcg
     4141 gcaacgaatt cttctccgcc aatacgccca agaatgtcat cggtacgtag agaagacttt
     4201 agcgtttcag cagcaaggat caaaaccgca tcccctattt tgtgcccgtg tttgtcgttg
     4261 aactgtttga aatcatccac atcgatcatg atgactgaaa aaggaatatt gaattgttct
     4321 gcacggacaa attcctgttc agctaattca aaaaagcgac ggcgattgag tattttcgtg
     4381 agcgagtctg tatttgccag gttttggatg gaatcaaata aacgggcatt ttcgatggca
     4441 attgcagcat gagacgcaag aacttccaat aaatataaat ggtctcggtt ataagcattt
     4501 gtattgtacg attgtgccga aaccataccg tacatatcac cgtgcaaggt cattggcaca
     4561 gccaaaatag actgactcgg gtcatcttca tgcgtgccat aaagttcaaa cttgatgccg
     4621 ctattgtcca tttcttcaac actgttaaaa agcagtgatt ttttggaaag aacaatctct
     4681 ccagccatac catggtcagc aagatatcga ttcgtaaaga ctcgcttacc ggggtattca
     4741 atagcataga ttggcacaat ctcgttcgta tctttatcat agccatcaat tacaaagtca
     4801 tcgcacggca tgacttgttt aacggtctcg tatacaacct ggcaaacccg ttccatttca
     4861 aggcttgcgt tgatggcatt aacggcggca tgaagaactt tcaagcgttc gactaaatca
     4921 tttacagcct cttgtgccca aatgcgctcc aaggctccgg cgcaatggct tgctagatct
     4981 ttcaataatt caagttgctc atctttataa aaatactttt gatagctctg aatggaaagc
     5041 acaccaattg tccgcgaacc gctgatgacc ggcacaaaca attgagataa agtcctttta
     5101 cttcgatctc cgaatgaagc cgttactcca atatcaaaag gctcgtcata gtgagacata
     5161 aagccgccgt gcgcaattgc cttcagcata ttttcactgg gcttttgcgg catggcattc
     5221 ttttgttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca attgccttca
     5341 gcatattttc actgggcttt tgcggcatgg cattcttttg ttctacatgg tttccattaa
     5401 tagtgtcgat tgtaagcaat gggcgtggaa caccaccgat ggcagggtca taaaggataa
     5461 gataagcggc atcccaacca ataaggctat ctgcggcatt taatatgatc tctgctgcag
     5521 accttgcttc agtcgtcgat gctagatcac gccccaagtt tgcaaaaacg gctgtttcac
     5581 ttcgagaatt ctgacgtgcc ggcttgctgc tcattttgct ctttgtcgta ctgcctcata
     5641 agtcattagt gcagtggcaa tcgaaacatt caaggagttt acttttcccg tcatgggaat
     5701 ttttacacgc aggtctgaat tctgcatcca gaaatcggtg agaccttcat cttctgtccc
     5761 aaccgcaatg gcaagcggac cttgcatatc aacatctgtg tacattgcat ctgcggatgg
     5821 agttgcagcc aatacacgca tcttacggga cctcaaccaa ctcagcgctt cagccgattt
     5881 cacctcgaca acaggtacgg caaagactgc tccgcggctg gcgcgaatgg tgtttggatt
     5941 ccacgtatcc acacgcgggt cgcagacgag aactgcatct acatgcgcgg catcggcagt
     6001 gcgaaggatg gcgccaagat taccaggctt ctctacagac tcagcgacaa tcactagcgg
     6061 attttctgaa agcttcaatt catccaagct ggatgttggc atagggaaaa gcccaagcca
     6121 accatcgggg ttatcacggt aggaaatctt ttcaaagacg gcacggctca ctgtcaaatt
     6181 ttcagcgttg ggaacagtta gggattggct aacaagctcg ggcgcggaaa gaagcgtttg
     6241 cggttcgaat ccacacgaaa gcgcgagagt caattcatcg tagccttcca ccagaatcaa
     6301 gcgatccttt tggcgctgct ttttatcctc acgcaatttg acaatatgct tgacgcgcgg
     6361 gttttgtaaa cttgtaatat ccatttattt tgtattcgtt actaacagac aagctgcctg
     6421 atgtgatttt gaaagggttg tcatgggcgg gtcactgagt ttgcaggcat caaaggctgc
     6481 cgggcaccgc ggatggaaac gacaacccga agggatatta atggggttgg gcgtttcacc
     6541 ttgaaggaca atgcgttcat gccgtaaacg cggattgggc acagggataa ccgagagcaa
     6601 cgctttggta tacggatgct gtggcttctc aagaacttct aacattgtac caatctctac
     6661 gattcgtccg agatacatca ctgctacacg gtcagcaaag aagccaaccg agccaagatc
     6721 atgcgtaata aaaatgactg cgatctgacg cgagatgcga agctctgcca gaaggttgat
     6781 cacttctgcg cggatggata catccaacat agacacgggt tcatcggcaa taatgatctc
     6841 tggctcaagc acaagcgcag atgcaatgac cacgcgttga cgttgccctc ctgaaagttc
     6901 gtgcgggtaa cgatgcaaat atacatctgc aggttttagc ccggcgtctt ccatggcttt
     6961 tcgcacacgt tcatcacggt cttgatgaga tgatgccata ccatgcacgg tcaacggttc
     7021 ggttacgatt tcttcaatgg tctgcgtggg gttcaaagac tcataggggt cttgaaaaac
     7081 catttgaatt tttcggcgta ggtcttttcg ttgatggtcg ttgagatgtg taatatcgcg
     7141 atattcaaaa ataaccgaac catcggtggg gtcttccaaa cccatcagca acaaacccaa
     7201 ggtcgatttg ccgcatccgc tttcgccaac gagggcaatt acttcgctgc gacgtaaggt
     7261 caggttcaca ccatccacag cgtggacatg tttcgtttcg cgtgaaaaca aggatgggcg
     7321 ccccgcttca aaatattttt tcagaccacg aatttccaaa agtttttcgg tagtattctt
     7381 cattttgatt tctcaatcag gtggcagctc actacatgcc cgtttgcgat ggtatgaaga
     7441 aggggttctt ctgtatgaca acggtcaaag gctgctgggc agcgcggagc aaagcgacag
     7501 ccttgcggca ggttatctaa acgcggcggg tagcccggaa tagaagagag ttttttcttt
     7561 ggcttcgtta aatctggaaa ggctttcaaa agttcttggg tataggggtg ttgcggagaa
     7621 ttgtagatta cgtctacatc agcatattca gcggtcacac cgccatacat aacaagcact
     7681 ttatcacata actcggcaac aacaccaaga tcgtgggtca caaagataac tgccaaccca
     7741 agtttcttgc gcaggctatc cagcaattcc aaaatttgtg cttggatcat tacatccaat
     7801 gctgtagtcg gctcgtcggc aatgattact ttcgggttac atgctaatgc catggcgatc
     7861 atggcacgtt gacgcatccc accagaatat tgatgcggga aatggtcgcg atgcgccgaa
     7921 gcgataccaa ccaattccag aagctgagaa acacggtcat tgatcttatc tttcggaaaa
     7981 gatggctcat gcttgagaat ggcttcccca atttggtcac cgacagtgcg cacagggttc
     8041 agcgcattca tcgcaccttg aaatacaatc gaaacaccgc tccaacgaac tgtattgatt
     8101 tcatcttcgt tcatggcagt gatatcttgc ccgctataaa aaactttgcc gttcacaatt
     8161 tgtccggcag acggtaatag ttttaacaac gccaacatca gtgttgtctt gccgcaaccg
     8221 ctctccccca ccaaaccgat caactcgccc gctttcaacg tgaaactaac attctccact
     8281 gcacgcgcag gcgcttttcc ttcggtcaca tagttgatag aaagattttg gacttccagt
     8341 aaagcatctt gctggatagg cttcgccccc gcctcattct ggacgacagg tttgcccggc
     8401 attaagtgat gtgtatccag ccgtgggttg agtacctgtt ccaagccctg cccaagcaaa
     8461 gtgagcgaga ggacaaccca tacaatgcca aagcccggag caaacaacgc ccaccatgca
     8521 cc
//
LOCUS       DAOQNJ010000039         8348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4302_length_8348_cov_10.032075, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000039 DAOQNJ010000000
VERSION     DAOQNJ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4302_length_8348_cov_10.032075"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLG52_02165"
     CDS             <1..231
                     /locus_tag="PLG52_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNB40273.1"
                     /translation="KLLIGPLVGLLLASLFGLQGHTRQGSVIEAAMPAAVATTVVATE
                     YKLEPSLVTAIVFFGTILSPITLTPLIVFLTR"
     gene            237..1469
                     /locus_tag="PLG52_02170"
     CDS             237..1469
                     /locus_tag="PLG52_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNB40274.1"
                     /translation="MSATRQRILQVFMVSLLVATLSLKPNQAQAQEGIVVENPNATPD
                     FGKTITFTAKIKASNPIQQASLLFRGVNEETTRVETLQVAEDGSVQFIYDASQNVFPP
                     FSWVVYWFQATLSDGKTYTSESNRVQYVDNRFPWRDVTQANVTIHWYAGDDAFGASAL
                     DNAGAGMLGMKDFIPLSLTEPIHIYIYSNVQDLGDTLMLGGREWAGGHAHADLGVVLV
                     AIAPGANQFTEMESLIPHELAHVMLYRALGDTYYKQPAWLIEGIASMVELYPNPDYAR
                     ALEIASVNDTLIPFSDMCASFPADSGSAFLAYAQSQSFVTYIRDSFGVSGLSRLISTY
                     GDGFDCDLGITKALGASLNQLDSRWRENVLGQNQVGAALRNLLPFLLLLLLVLIVPIW
                     GAVDVMRRRRKKYAAKSK"
     gene            1450..1752
                     /locus_tag="PLG52_02175"
     CDS             1450..1752
                     /locus_tag="PLG52_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNB40275.1"
                     /translation="MQPNPNEPVRVHIWVKGRVQGVGFRAHVEYSARQIGGLSGWVRN
                     VGDDTVEAIAEGQREQVERLIEAMKQGPRMSRVDEAKIDWESPTGEYNGFRVKSSA"
     gene            1756..3381
                     /locus_tag="PLG52_02180"
     CDS             1756..3381
                     /locus_tag="PLG52_02180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB40276.1"
                     /translation="MFSNPYSPYLFTSAITSFVMAMLLWRRRIAPGATPLSVLLGLTS
                     VWALASALLLESSSTLLRELWFDILSISAMMTTPMLLIFCLEYTERQNFLSKRLLLFV
                     YLFPILNAILCVTTNYHHLFYAEVDFSYQPANFLALVPGPFYWIYTVYSYGIALFIFV
                     LILTNFFQTSSTYRKQTATILLGLLFPFVGNVIYTLLLGVGKGAFDPTPMLFTVMGFF
                     LVCGLLWFQLFDVIPVARHTLVEHMQDGVIVVDVKNRIVDVNPSALKWLNWEGKSSPI
                     GKNVEELLTSWFEQFANLPIDLYVQTEVHKEGTPGLYFDLRVEPIYNRQNVLTGRLIV
                     FRDITRQKMSEKALLEAHDRLRLHLKEIELLQSELREQVIRDPLTGLFNRRYMEETLE
                     REISRAIRENISIGVCMADIDQFKSFNDEHGHKAGDLILKKLSEILTNYSRAEDVVCR
                     YGGEEFLILMPGAELDAIARRAEDWRRAFEQAKVEFDGKQLSTTLSMGIAMFPQQART
                     SDDIVKLADEAMYLSKHNGRNQVSIAQTWGEKR"
     gene            complement(3378..>3596)
                     /locus_tag="PLG52_02185"
     CDS             complement(3378..>3596)
                     /locus_tag="PLG52_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNB40277.1"
                     /translation="VGELFKDAIRANASAIIVAHNHPSGDPTPSPDDVAVTRAIVQAG
                     KLLDVDVLDHLVIGQAKWVSLKERGLGF"
     assembly_gap    3597..3696
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3697..4240)
                     /gene="radC"
                     /locus_tag="PLG52_02190"
     CDS             complement(<3697..4240)
                     /gene="radC"
                     /locus_tag="PLG52_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNB40278.1"
                     /translation="MTDSTNSKPTYRISDLHEADRPRERLAALGPQALTNAELLAILL
                     RVGVQGENAVEVGQRLLKKFNGLNGLHRAPFKELMDQHGLGEAKAAQIKAAIELGRRL
                     TLEAPQERPAINSPADAAALIQYEMSALEQEHLRVILLDRRNRVLEVVEVYKGSVNSS
                     QVRVGELFKDAIRANASAIIV"
     gene            complement(4326..5411)
                     /locus_tag="PLG52_02195"
     CDS             complement(4326..5411)
                     /locus_tag="PLG52_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40279.1"
                     /translation="MKSFLRTLVLTLILTALITACGLPAGTADPDVVSTAIAQTQMAQ
                     VLAQATVNSNTLTAMPATPTPGPTVEYVTLTEEELAALIDQAVAEAIAATEQTTTNVY
                     YVTEDDTVTTEEVEYVYSYYYYADYYVDYAEELMAEYYNLYSDLAYEMIDELNAIESE
                     LNQMNDTLSSIDQSLQQISSTLEQGLELAEDTITQLETAAQNAQTNAQELKSQAQDMM
                     TVLQADQQGRLDQIGQIQPDNIPTDKLSALQTAFSFVDFANQALSDNKLTRDELTNLM
                     QLGKNAQAGFQQFGGADGVGPDVTQFAGKFDEIGGQFARGEINHGRENLHSFEGSLGN
                     RPSFGNGQGGPSGNGPGGGGGLPGPRP"
     gene            complement(5433..6746)
                     /locus_tag="PLG52_02200"
     CDS             complement(5433..6746)
                     /locus_tag="PLG52_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNB40280.1"
                     /translation="METNLVLEQPVYKTSTFEFLWNVFRNPLHALTDLAHEQGDIARV
                     KLRKRDLFLLSHPAFIEQVLVRQADNFVKGPSLQRARIILGEGLLTSEGDEHLTQRRT
                     LQPAFHRHKMEEYIPLMNENTMAHIANWQDGERADMSEGMMRLTLNIALWSFFGNAPS
                     GTVDKVSDAMKTLVRLFPLAQMPLPESARSLFPSFKEAKADLSEVTEALLKNPNSEYA
                     KRALVNILKENHKDQFTDEQVHAHALTFLLAGHETTALLLAWCWDLLAHHPMAQAILQ
                     AEVDSVLGDRLPTPEDIQKLPYTRAVVKETLRMRPPAWAMGREALHDCEIGGQFIPAG
                     STVIMSQWVTHHDSRFYYEPLHFRPERWFDLEGTSTPRYSFFPFGGGNRVCIGEHFAM
                     TEAIAILAVVARRWNISPVQRELATPKPSVTLRPEKNLNVIVTKR"
     gene            complement(6890..7528)
                     /locus_tag="PLG52_02205"
     CDS             complement(6890..7528)
                     /locus_tag="PLG52_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB40281.1"
                     /translation="MLKIIIADDHQVVRRGLTLTIDAEKDMRVVGEAQNGAGVLALIK
                     KHKPDVVLMDLQMPEMTGVDALKQIRPEYPDLPILILTSFTDDAHVFAALRAGASGFL
                     LKEMNGDDLVAAIRGAAQGKPQLHPDIAKRLMARAPMPDDPFDSLTERERGILKLLAK
                     GKSNKEIAGELSLTEMTVKGYVSDMFVKLGVNDRTQAALMAVRYGLVLPEEL"
     gene            complement(7531..>8348)
                     /locus_tag="PLG52_02210"
     CDS             complement(7531..>8348)
                     /locus_tag="PLG52_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB40282.1"
                     /translation="GYWLGPRQAGPQFDGDEVKRLNALAGQAALALAYSNAYESLYEL
                     NKNLETRVKEQTEQAISDQKSIAAYEERQRIARDLHDSVTQQMFGLHLMARGLKKSAP
                     EDFKDKLAELESLASDTLKEMRLMLDQLREASGEEKVDFAESVRAECEAFSNRSGPEG
                     GALLSVTLEMPQVVIIPKNIASEATWVLREALQNVVKHSGGRSVTVRVSVDAFLHVSV
                     TDDGAGFDVHAMPTGHYGVRGMRERVLALGGELTIDSEIGRGTILKYKIPLPR"
BASE COUNT         2009 a         2137 c         1931 g         2171 t
ORIGIN      
        1 aagttattga tcggaccgtt ggttgggtta ttgctggcaa gcctgttcgg gttgcaaggg
       61 catacccgtc agggcagtgt catcgaagcc gccatgcccg ccgcggtagc aacaaccgtt
      121 gttgctaccg aatacaaact tgaaccgtct cttgtgacag cgattgtttt cttcggcacc
      181 atactgagtc cgattaccct tacaccgttg atcgttttcc ttacgaggta atgaacatgt
      241 ccgccacacg tcaaaggatt ttgcaagtct tcatggtctc acttctcgtg gcgacccttt
      301 ctctgaaacc gaaccaagcc caggcgcaag aaggaatcgt tgtcgagaat ccaaatgcga
      361 cccccgattt cggaaaaacg atcacattca cagcaaaaat caaagcatcc aatcctattc
      421 aacaagcatc gctcttgttc cgcggggtca acgaagaaac gacccgtgta gaaaccttac
      481 aagttgcaga agatggctcg gtacaattca tctatgacgc ttcgcaaaat gttttccctc
      541 cattcagttg ggtcgtctat tggtttcaag caacattaag tgacggcaaa acttacacca
      601 gcgaatcaaa tcgcgttcaa tatgttgata atcgattccc atggagagat gttacgcaag
      661 ccaacgtcac cattcattgg tatgccggcg atgatgcttt tggtgcaagc gcattagata
      721 atgccggtgc aggcatgttg gggatgaaag attttattcc gctctcactt accgaaccca
      781 ttcatattta tatctactcc aacgtccaag accttggcga tacgctgatg ctcggcgggc
      841 gcgaatgggc aggcggacac gctcatgcag accttggcgt ggtattggtt gcaattgcgc
      901 ccggcgccaa tcaattcacc gaaatggaat ctctaatccc acatgaactt gcacatgtca
      961 tgctatatcg cgcgcttggc gatacttact acaaacaacc tgcctggctg attgaaggca
     1021 ttgcctccat ggtggagctt taccccaacc ccgattatgc gcgtgctttg gaaattgcca
     1081 gtgtgaatga taccttgatt ccattttcgg atatgtgcgc ctctttcccc gccgattctg
     1141 ggtcagcatt tctggcatat gcacaatctc aatcctttgt cacctacatc cgcgactcgt
     1201 tcggggtctc gggtctctca cgcctgatta gtacttacgg tgacggcttc gactgtgacc
     1261 tcggcattac caaagcctta ggtgcttcgc tcaaccaatt ggattcacgt tggcgtgaaa
     1321 atgtgctcgg gcaaaatcaa gtaggcgcag cgttacgaaa cttattgccc ttcctacttt
     1381 tacttcttct tgttttgatc gtaccaatct ggggagccgt tgatgtgatg cgccgaagga
     1441 gaaagaaata tgcagccaaa tccaaatgaa ccagtccgtg tacacatttg ggtgaaggga
     1501 cgggtgcaag gggttgggtt tcgtgcacac gtagaatatt cggcgcgcca aattggaggg
     1561 ttaagtggct gggtgcgcaa tgtaggggat gatacagtgg aagccatagc tgaaggtcaa
     1621 cgagaacagg ttgaacgttt gatcgaggcg atgaaacaag gtccgcgcat gtcacgggtg
     1681 gatgaggcaa aaatcgattg ggaatcgccc acaggggagt acaatgggtt tagagtaaaa
     1741 agcagcgcat aagttatgtt ttcaaaccca tactccccct acctatttac ctcagccatc
     1801 acctcatttg tgatggcaat gttgctgtgg cgtcgacgca tagctccggg ggcgactcca
     1861 ttatccgtcc tcttgggtct cacttcagtt tgggcgttgg catcggcttt gttgctagag
     1921 tcgtccagta cgttactcag ggagttatgg ttcgatatat taagcataag cgcaatgatg
     1981 accaccccta tgctgcttat cttttgtttg gaatacacag agcgccaaaa cttcttaagt
     2041 aaaagattgt tactctttgt ctatttattt ccgattctta atgccattct gtgtgtcaca
     2101 acgaattacc atcacctgtt ctatgcagag gttgattttt cataccagcc tgctaatttt
     2161 cttgctttgg ttccgggtcc attttattgg atatacaccg tgtattccta tggcatagca
     2221 ctttttatat ttgttcttat ccttacgaat ttttttcaaa cctcttcaac ttatcgcaag
     2281 caaacagcca ctattctact tgggctattg ttcccattcg ttgggaacgt gatttacact
     2341 ctgcttcttg gggttggcaa aggtgcgttt gaccccacgc ctatgttgtt tactgtgatg
     2401 ggattcttcc ttgtatgtgg gttgctctgg tttcagttat tcgatgtgat cccagttgcg
     2461 cggcatacac tggttgaaca catgcaagat ggcgtgattg tagtggatgt aaaaaatcgc
     2521 atcgtagacg tgaatccatc cgccttgaaa tggctgaatt gggaaggcaa atcatcccct
     2581 attgggaaaa atgttgaaga acttcttact tcgtggtttg aacaattcgc aaacttaccc
     2641 atcgaccttt atgttcaaac agaagtacat aaagaaggca ctccagggtt gtattttgac
     2701 ctgcgtgtag agcctatcta caataggcaa aacgttctca cgggtcggct gatcgtgttt
     2761 cgggatatca cccgccagaa aatgtctgaa aaggctttat tggaagccca tgaccgctta
     2821 agactccatc tgaaagagat tgagcttctg caaagtgagt tgcgcgaaca agtcatccgc
     2881 gacccgctga cggggttgtt caaccgtcgt tacatggaag aaacattgga gagagaaatt
     2941 tcgcgagcca ttcgcgagaa tatttcaatc ggggtttgta tggcagatat tgaccagttc
     3001 aagtctttca atgacgagca tggtcataaa gctggtgact tgatcttgaa aaagctatca
     3061 gagatattaa ccaattactc gcgcgccgaa gatgtcgtct gtcgatatgg aggcgaagag
     3121 ttcttaatcc tcatgccggg cgcggagttg gatgccattg cccgccgcgc cgaagattgg
     3181 cgcagagcat ttgagcaagc aaaagtagaa tttgatggga agcaactctc taccacgtta
     3241 tccatgggca ttgccatgtt ccctcaacaa gcgcggacaa gtgatgacat cgtcaagttg
     3301 gcagatgaag ccatgtatct ttctaaacac aatggcagaa atcaagtaag tattgcacaa
     3361 acatggggcg aaaaacgcta gaaacccaaa ccgcgctctt tcagtgaaac ccactttgcc
     3421 tgaccaatca ccaaatggtc gagcacatcc acatccaata gtttcccggc ttgtacaatg
     3481 gcacgtgtca cagctacatc atctgggctg ggagtggggt ctccgctcgg atgattatgt
     3541 gccacaataa tagcggaagc attggcacgg attgcatctt tgaataattc ccctacnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccac aataatagcg gaagcattgg
     3721 cacggattgc atctttgaat aattccccta ctcgcacttg tgatgaattg accgaccctt
     3781 tatagacctc aacaacttcc agcactcggt ttcttctatc caacaatatc actcgcaaat
     3841 gttcctgctc caaagcggac atttcatatt ggatcaatgc cgccgcatct gccggactgt
     3901 tgatcgcggg tcgctcctga ggcgcctcaa gagtcagcct acgccccagt tctatggcag
     3961 ccttgatctg cgccgctttg gcttcaccca acccatgttg atccatcagt tccttaaatg
     4021 gtgcacgatg taacccattc aagccattaa actttttcaa cagacgttga cccacttcta
     4081 cagcattttc accctgcacg cccactcgca acaaaatagc gagcagctct gcattcgtca
     4141 gcgcctgcgg acccaatgcc gcaagccgct cacgcggacg gtctgcctcg tgcaagtctg
     4201 agatacgata ggttggtttt gagttggtgg agtctgtcat ttcgcctccc ttgagataag
     4261 ttgaccaatt ttacagccaa ttcagaaaaa agtctgctga catttttatc agcagacttt
     4321 ttcatttacg ggcgcggacc gggcaagccg ccgccacctc caggtccatt tccgctgggt
     4381 ccaccctgac cgttcccaaa tgaagggcgg ttgcctaagg aaccttcaaa actatgcaag
     4441 ttttcacgtc catggttgat ctcgccgcgt gcaaactgcc cgccgatttc atcgaatttg
     4501 cctgcaaatt gtgtgacgtc aggtccgaca ccatctgcgc cgccaaattg ctggaaacca
     4561 gcttgggcgt tcttgccgag ttgcataagg ttcgtcaatt catcacgggt aagtttatta
     4621 tcgctgagtg cttggtttgc aaaatccaca aaggagaaag ccgtttgcaa tgcggacagt
     4681 ttatcggttg ggatattgtc cggctggatc tgaccgatct ggtccaaacg accttgctgg
     4741 tcagcttgta aaacagtcat catatcctgt gcttgagatt tcaactcttg cgcattggtc
     4801 tgggcatttt gagcagcagt ttcaagttga gtaatggtat cttctgccaa ttccaatcct
     4861 tgttccagtg tggaagaaat ctgttggaga gattggtcta tggagctaag cgtatcattc
     4921 atctgattta gctcagattc aatggcgttt aattcatcga tcatttcata agccaggtct
     4981 gaatacaagt tgtagtactc tgccattagt tcctcagcat aatccacata gtaatcggca
     5041 tagtaataat agctgtatac atactccacc tcttcagttg tgaccgtgtc gtcttctgtc
     5101 acgtaataca cattggtcgt ggtctgttcg gtggcggcta tggcttctgc aacggcttgg
     5161 tcgatgagcg cagcaagttc ttcttcagtg agtgtcacat actcaaccgt gggtcccggg
     5221 gttggagtag cgggcatggc tgtcaacgta ttggaattga ctgtagcttg agcaagaact
     5281 tgcgccattt gtgtttgagc aattgcggtg ctgacaacat ccgggtcggc ggtgccagcg
     5341 ggtaacccac aggcagtgat aagagccgtg agaatcaggg tcaatacaag ggttcgaagg
     5401 aaagatttca tgttatatct ccaatttagt ttctatcgtt tcgtcacgat cacatttaga
     5461 tttttttcag ggcgtagtgt cacactgggt ttcggtgtgg caagttcgcg ctgaaccggt
     5521 gagatattcc aacgtctcgc aacgacagcc agaatggcaa tggcttcggt catggcgaaa
     5581 tgctcaccaa tgcagacgcg attgccgccg ccgaagggga agaaactgta acggggagtt
     5641 gaagttcctt cgaggtcgaa ccagcgttcg gggcggaagt gaagcggttc gtaataaaag
     5701 cgtgagtcgt gatgggtcac ccactggctc ataatcacgg tcgaacccgc cgggatgaac
     5761 tgcccgccaa tctcgcaatc atgcagggct tcgcgcccca tcgcccaagc cggtgggcgc
     5821 attcgcaagg tctctttcac aaccgcacgc gtgtatggca gtttttgaat atcttcgggc
     5881 gtgggcaaac ggtcacctaa aaccgagtcg acttccgctt gcaaaatagc ttgcgccatc
     5941 gggtgatgcg cgagcaggtc ccaacaccag gcaagcagca aagctgtggt ttcatgccca
     6001 gccagcaaga acgtgagcgc atgcgcgtgg acctgctcat cggtaaattg gtctttatga
     6061 ttctctttga ggatattgac caaagcccgc ttggcatatt cagagtttgg attcttgagc
     6121 aacgcctctg tgacttcact caggtctgct ttggcttcct tgaagcttgg gaataagctg
     6181 cgagcggact ctggcaaagg catttgcgcc agcggaaaga gacgaaccaa ggtcttcatc
     6241 gcatcgctga ctttatccac agttcctgat ggcgcattgc caaagaatga ccacaacgcg
     6301 atattaagcg ttaatcgcat cataccttca gacatatctg cccgctcgcc atcttgccag
     6361 ttcgcaatat gtgccatcgt attctcgttc atcaagggaa tgtattcttc catcttgtga
     6421 cgatgaaacg cgggttgcaa ggtgcgacgt tgagtaaggt gttcatcccc ttcgctggtg
     6481 agtaaccctt cgcctaaaat gatgcgtgcc ctctgcaagc tgggaccttt cacaaagtta
     6541 tctgcctgcc gcaccaagac ctgttcaata aatgccgggt ggctcagtag aaagaggtca
     6601 cgcttgcgca atttgactcg tgcaatatca ccctgttcat gcgcgagatc ggtcaacgca
     6661 tgcaacgggt tgcgaaagac attccataaa aattcaaagg tggatgtttt gtaaacgggt
     6721 tgttcaagaa ctaaatttgt ttccatcggc tatctccttc aagtgataac caaaggatac
     6781 ttttttgaac cgattaagtc cgcccgcgaa ggtggggaac ctctccccct atttggtggg
     6841 tatataaaaa aagtgacagt caccttaaag gtgactgtca ctttgagttc tacaattctt
     6901 ccggcaaaac caacccatag cggaccgcca tcagcgccgc ctgagttcta tcgttcaccc
     6961 ccaacttcac gaacatgtca ctgacatagc ccttcaccgt catctcggtc agcgataact
     7021 cgccagcaat ttctttattg ctcttaccct ttgccagcaa tttcaaaatg ccgcgctcac
     7081 gttcggtcaa cgaatcaaac gggtcatcgg gcatgggagc gcgcgccatc aggcgcttcg
     7141 caatatcagg gtgcaattgc ggtttgcctt gcgccgctcc acggatcgct gccaccaaat
     7201 catcgccatt catttccttc agcaaaaatc cgctcgcacc cgcacgcaac gccgcaaaga
     7261 catgcgcatc atcagtgaac gaagtcagaa tcagaatcgg caaatcaggg tattccgggc
     7321 ggatttgctt caatgcatcc acaccggtca tctcgggcat ttgcaaatcc atcaagacca
     7381 catcgggctt atgttttttg atcaacgcca acacacccgc accattctgt gcctccccca
     7441 ccacacgcat gtctttctcg gcatcaattg ttaaggtcaa cccacggcgc acaacctgat
     7501 gatcatcagc aataattatt ttcagcatac tcacctcggc aaaggaatct tgtacttcaa
     7561 tatcgtaccg cgtccaatct cagaatcgat cgtcaactcg ccacccaacg ccaacacacg
     7621 ctcacgcatc ccgcgcacac cataatgtcc cgtaggcatg gcatgcacat caaaccctgc
     7681 gccatcgtct gtcacactca catgtaaaaa cgcgtcaaca ctcacgcgca ctgtcacact
     7741 ccgcccgccg ctgtgcttca ccacattttg caatgcctca cgcagcaccc acgtcgcttc
     7801 actggcaata ttttttggga taattacaac ttgcggcatc tcaagcgtta cagacagtaa
     7861 tgctccgcct tctggtccgc ttcggttgga aaaagcctca cactccgccc gaaccgattc
     7921 agcgaagtca accttttcct cgccggatgc ttcgcgcaat tgatcgagca taagccgcat
     7981 ctctttgaga gtatcacttg ccaggctctc caactctgcc agcttgtctt tgaaatcctc
     8041 cggcgcagac tttttcaatc cgcgcgccat aaggtgcaga ccgaacatct gctgcgtgac
     8101 cgagtcgtgc aaatctctcg caatgcgttg acgttcttca tacgccgcaa tggacttttg
     8161 atctgagatg gcttgctcag tttgttcttt cacccgcgtc tcaagatttt tgtttagctc
     8221 atacaggctt tcataggcat tggagtacgc caatgccaac gccgcctgcc ccgccaatgc
     8281 attcaagcgt ttgacttcat caccgtcaaa ctgcggaccg gcttggcgcg gtcccagcca
     8341 gtagccgc
//
LOCUS       DAOQNJ010000040         8338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4317_length_8338_cov_11.622963, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000040 DAOQNJ010000000
VERSION     DAOQNJ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4317_length_8338_cov_11.622963"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLG52_02215"
     CDS             complement(<1..373)
                     /locus_tag="PLG52_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40283.1"
                     /translation="MSSLADKLKSLGVKTANTLPTPPKPESQSIDSVVAGSFISTPRG
                     EAFVAEQAYDDTYLHGRFSPFSSFPLSFISQWANDPRIDQLPITKYAFLDTETSGVSG
                     GTGTYAFLVGAARFVDGKFVIK"
     gene            431..1858
                     /locus_tag="PLG52_02220"
     CDS             431..1858
                     /locus_tag="PLG52_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40284.1"
                     /translation="MSRKLPFWLKTLYGSGDWGIASISMMRSIFYAIYLTDVVGIEPR
                     LASLGALVGIVWDAVNDPIIGMLSDRVKSKLGRRRPFLLWFAFPFGLSFVMLWSAPNW
                     DSQVALLIYVTLAFMISDTLTTLVAVPYYSLTPELTQNYDERTSLSSFRSVFQLLSAM
                     VVVVSAPMIVDAVISGGGTQQQGFLTAGAVFGAIGSLPLFLIGLFIRERFAEDTQEQE
                     ELSFRETLKLAWQNVPFRYAVGIYMFNWSAVDMVSITFPFFLLYWVAQGDLLAKINIL
                     GVDLALESAFFGVLMLVCVLFVPFWLWLSKRQNKIRAYILGMAAWVVVQVLIFTIQPG
                     ETSYMLFLAGLAGIGVSAAYILPDSILPDVIEWDELRTGRRQEGIYYGIRTLIRKLTG
                     ALIIFITLQILGWSGYQAPPENVTVYQQPESALFMIRLMDSFIGAAILAGTIILAWSY
                     PLTREKYQKIQKLLAVRRNKNAETT"
     gene            complement(1894..3573)
                     /locus_tag="PLG52_02225"
     CDS             complement(1894..3573)
                     /locus_tag="PLG52_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB40285.1"
                     /translation="MYDKKKLDEIQQSLSKWEETSLKKALNQLPERADEFITTSSEPI
                     NRLYTPLDTADLDYNDSLGNPGEYPYTRGVHPTLHRSKLWTMRMFAGFGTAEETNGRF
                     KYLLEQGQTGLSIAFDLATLMGYDTDQPEALGEFGKCGVAVSSLKDMEILLDGIPLDK
                     VSSSMTINSPASIIWAMYLVAAEKQGVRLDQLRGTLQNDILKEFIAQKEFIFPPEPSM
                     RLVVDTMEFGSKHVPQWNTISISGYHIREAGSTAAQELAFTLADGMEYVRWGIARGMD
                     VDEFAPRLSFFFNAHNDFFEEIAKYRAARRIWAREMRETFKAKNPRSWLMRFHTQTAG
                     VSLTAQQPENNIVRVAIQALAAVLGGTQSLHTNSMDEALALPSEHAVKVALRTQQIIA
                     EESGVTNTVDPLGGSFFVEAQTDRMEKDAYAYFQRIQDLGGVIPALEKGFMQSEISDA
                     AYRYQREIDENRRKIVGVNAYLDEKPAAIPILKMDPNGYSRQMNRLNEVRKTRDSGRV
                     GQTLDALRIACEGTENTMPYILDCVRAYCTLGEIIGVMTKVFGKYEEPTMI"
     gene            3729..4256
                     /locus_tag="PLG52_02230"
     CDS             3729..4256
                     /locus_tag="PLG52_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HNB40286.1"
                     /translation="MSRTDIDVDPCDHITADAIGKPGQRVFYLHAYSEHRTYTIIIEK
                     AQLLSLAIGVEQFLGQLSQQYPDLEEASGDYVEEVMRINPPVDPLFRAGEIGLGYDKD
                     RDRVVVFAKELITEEQDPEEASQIRFWATRTQLRQLARWGQEVSQRGRPICPQCGQPM
                     EAGGHFCPKKNGHLH"
     gene            4297..4992
                     /locus_tag="PLG52_02235"
     CDS             4297..4992
                     /locus_tag="PLG52_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HNB40287.1"
                     /translation="MTDIKTILTHGNYELRGQFMLGSNYTFLVDVHHEDEVVKAVYKP
                     SKGEQPLWDFPDNTLALREVAAYELSETLGLHIVPVTVYRDDGPHGPGSLQQYIEYDI
                     EYHYFNFTVEDKQKLRPVVFFDILANNADRKGSHVFFEDETHRLYAIDHGICFHEENK
                     LRTVLWDFAGQAIPDELLAPLSLTGNWSALFEPYLSPREIDALLFRAEELCDSKVFPR
                     PLQGRRAYPYPPI"
     gene            5113..6147
                     /locus_tag="PLG52_02240"
     CDS             5113..6147
                     /locus_tag="PLG52_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNB40288.1"
                     /translation="MHYNIALIGFGNVARSLARLLVRKQDVLKSKYDVTFSFTGISTG
                     RHGYAVNQSGLDIQKALDLVESGKDISELSVFPVNDSISVINNSAANVMFENSPVNTQ
                     TGQPAVDHIRTALNLGMHAITANKGPVVHGYRELTALAESKGKTFRHESAVLGGAPVF
                     SVMREAFPLADLESFKGIFNATTNVILSRMESGETYEQALAYAQKIGLAETDPTNDVD
                     GWDAAIKVASLVTVLWDTPMTPQQVNPTGIRGITSEMIANAKKEGKRYKLVCSAERTD
                     GKIVASVSPQLVDSSSSLYGMMNSSTGITFRTDVIIDYSIVLSEKPGMTGGPVETAYG
                     LFADFVNIVR"
     gene            6226..7764
                     /locus_tag="PLG52_02245"
     CDS             6226..7764
                     /locus_tag="PLG52_02245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB40289.1"
                     /translation="MTPPNILYSTALLTAGICCFIVGLLVLQRRPFMPGSIPLMVFIL
                     AMCWWDISYGIFWGGVLKPSPYFWLDISYIGVVIVPAAFFVFAMQISGRQEWLKTPLL
                     IGLYVEPLLVLVLMWTDPWHNLFFAGKRLENTGMILNAGPVFWANVVYSYLLLLVTFI
                     LLVRRFTTTSGIYRRQLGVVIAGAGVTWLNSFIFVTGLSPFPNADNTPFSFTIAAFAF
                     AYALLRYRMLDIVPVARDTLIEGMSDGVLVLDTNNRIVDMNPEAEKLLSASSAGTIGR
                     HVEEVFAPWADLVKTFMNVDQTRVEVLASRDSDTYLDLQISPLFDKRKNFIGRLVMWR
                     DISRLKKIQTELEKLATQDPLTHVYNRRHFYTLAEVEIHRSLRSQLPFSVILVDIDYF
                     KSINDKYGHKAGDEALIHFTKICIQNLRKVDIFARFGGEEFVILLPEADLDQAKETAE
                     RIRKIVESTVLNIDEHQIKITASFGVSTFKGSPDTLDLLLQKSDEALYAAKNTGRNRV
                     VCAD"
     gene            complement(7766..8113)
                     /locus_tag="PLG52_02250"
     CDS             complement(7766..8113)
                     /locus_tag="PLG52_02250"
                     /inference="COORDINATES: protein motif:HMM:NF018672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNB40290.1"
                     /translation="MKLVSDILIVIVALLHFFFLYLEMFLWDKPRGMKSFNLTEEFAK
                     QSKRLAMNQGLYNGFLAAGLLWGLFAGNPVKIFFLSCVIIAGIFGAFTVSKRIFYIQA
                     VPAIVALVILLVA"
BASE COUNT         1998 a         1983 c         2087 g         2270 t
ORIGIN      
        1 gcttgatgac gaacttgcca tctacaaaac gggcggcacc cacgaggaaa gcataggtgc
       61 ccgtcccacc agagacgcca gacgtttctg tatctaaaaa ggcatacttg gtgatgggga
      121 gctggtcaat gcgtgggtca tttgcccatt gcgaaataaa cgaaagcgga aacgacgaga
      181 acggtgaaaa ccgcccgtga agataggtgt cgtcataggc ttgttctgcc acaaaggctt
      241 cgccgcgcgg cgtggagata aaactcccag cgacgactga atcaatggat tggctctcag
      301 gcttgggcgg ggtgggaagc gtgtttgccg tcttcacgcc gagtgattta agtttatccg
      361 ctaaggagga catgtctaat attttatcgc tattaaccgt gatacacttg cgcgaaattt
      421 tgaaggtgcg atgagcagaa aactcccatt ttggttgaag actttatatg gttcgggtga
      481 ttggggaatt gccagcatta gtatgatgcg ctcgatcttt tatgccattt acttgacaga
      541 tgtggttggc attgagccac ggctggcatc gttgggtgca ttggttggaa ttgtgtggga
      601 tgccgtcaat gacccgatta ttgggatgct gagtgacagg gtgaaatcaa aacttggcag
      661 gcgtaggccg tttttgctgt ggttcgcctt cccattcggg ttgagttttg tgatgttatg
      721 gtctgcaccg aattgggata gccaggttgc tttgttgatc tatgtgacgt tggcgttcat
      781 gatctcagac acgctgacaa cgttggtggc ggttccatat tattcgctca cccccgagtt
      841 gactcaaaac tatgatgagc gtacatcgct ttcaagtttt cgctcggtct ttcaattgct
      901 ctctgcaatg gtggtggtgg tttctgcacc catgatcgtg gatgccgtga tttcaggcgg
      961 cggcacgcaa caacaaggtt ttctcacggc tggggcggtg ttcggcgcga ttggttcgtt
     1021 gccattgttc ttgattggtc tttttatacg tgaacgattt gcggaagaca cacaggaaca
     1081 agaggaactt tcatttcgtg agaccctgaa actggcttgg cagaacgtgc catttcggta
     1141 tgcagtggga atttacatgt tcaattggtc tgccgtggac atggtctcga tcacatttcc
     1201 gttcttcctt ttgtattggg tggcacaggg agatttattg gcaaagatca atatccttgg
     1261 tgtagacctt gctttagagt ctgccttctt tggggtgttg atgttggttt gtgtgttgtt
     1321 cgtgccgttc tggctgtggc tatcgaaacg acaaaataag atcagagcgt acatccttgg
     1381 tatggcggcg tgggttgttg tgcaggtgtt gatcttcact attcaaccgg gcgaaaccag
     1441 ctacatgttg ttccttgccg ggttggctgg tattggcgtt tctgccgctt acattttgcc
     1501 agactcgatt ttgccagatg tgattgaatg ggatgaactt cgtacggggc gcagacaaga
     1561 aggtatttat tacgggatcc gtacgctcat ccgtaagttg accggggcgt tgatcatttt
     1621 catcacgctg caaattttgg gttggtctgg ctatcaggcg ccgccagaaa atgtgaccgt
     1681 gtaccaacaa ccggaatctg ccttgttcat gattcgccta atggactcgt tcatcggcgc
     1741 agcaattttg gcgggcacga tcattctggc atggtcttat ccgctcacac gtgaaaaata
     1801 tcagaagatt caaaagttac tggctgttcg ccgaaacaaa aacgcggaaa cgacttgatc
     1861 gtttccgcgt tttgctataa aaggcgagag cgtctagatc atcgttggtt cttcgtactt
     1921 tccaaaaacc ttcgtcatca caccgatgat ttcccctaat gtgcaatagg cacgcacaca
     1981 atccaaaata taaggcatgg tgttctcggt tccttcgcaa gcgatgcgca aagcgtcgag
     2041 agtttgccct acgcgaccac tatcgcgcgt tttgcggact tcgttcaggc gattcatctg
     2101 acggctgtat ccgttcgggt ccattttgag gattggaatg gcggcaggtt tttcatcgag
     2161 atacgcattg acgcccacga tcttgcggcg gttctcatcg atctcacgtt gataacgata
     2221 cgccgcatcg gagatttcgc tttgcatgaa acctttttct aacgcgggga tgactccacc
     2281 tagatcttga atgcgctgga agtaggcgta cgcatctttc tccattcggt ctgtttgcgc
     2341 ttcgacgaag aaactacccc caagcgggtc gaccgtattc gtcacgccgg attcttctgc
     2401 gatgatctgc tgcgtccgca gagcaacttt cacagcatgt tcagagggca gtgcaagcgc
     2461 ttcatccatc gagttggtgt gcaaactttg cgttccaccc aataccgcag ccaatgcctg
     2521 aatcgccacg cggacgatgt tattttccgg ctgttgtgcc gtgagcgaga cacccgctgt
     2581 ttgagtgtgg aagcgcatca gccacgagcg cggattcttc gctttgaagg tctcgcgcat
     2641 ttctcgcgcc caaatacggc gagccgcacg atacttggca atctcttcga agaaatcgtt
     2701 atgagcattg aagaagaaag agagacgcgg cgcaaattca tctacatcca tgccgcgtgc
     2761 aatgccccag cggacatatt ccatgccgtc tgcaagggtg aacgccagct cttgcgcagc
     2821 cgtcgaaccc gcttcacgga tgtgatagcc cgaaatggaa atggtattcc actgcggcac
     2881 gtgttttgag ccgaactcca tcgtatccac tacgagacgc atggaaggtt ctggtgggaa
     2941 gatgaattcc ttctgtgcga taaattcttt gagaatgtcg ttctgaaggg tgccgcgtaa
     3001 ttggtcgagg cgcacacctt gtttttcagc ggctacaaga tacattgccc agatgatgga
     3061 agcgggcgag ttgatggtca tgctcgaaga aactttatcg agcggaatgc catccaaaag
     3121 aatttccata tctttaagcg aagaaacggc tacaccacat ttgccaaatt cgcccagcgc
     3181 ttcgggttgg tcagtatcgt agcccatcag cgtggcaagg tcgaaggcaa tgctcaagcc
     3241 ggtctgaccc tgctcaagta agtatttgaa gcgcccgttc gtctcttctg cagtaccaaa
     3301 gcctgcaaac atacgcatgg tccacagttt ggagcgatgc aacgttgggt gaactccacg
     3361 tgtgtatgga tattcacctg ggttgccgag cgagtcgttg taatctaaat cggcggtgtc
     3421 gagcggtgta taaagtctgt tgatcggttc ggaggaagta gtaataaatt cgtcagcacg
     3481 ttcagggagt tgatttaaag cttttttgag agaagtctct tcccacttgg aaagcgattg
     3541 ttgtatttca tcgagctttt ttttatcgta catggcatct cctttggcga gttgatgaga
     3601 ttataccgcc ccctcctcct actatcacgg gcatctcttc ccatatgtaa tgaattaact
     3661 ttcctcacat tttagggagg atgggtgggg atggctattc tggtatactc tcaaaaaagg
     3721 taatctttat gtctcgcact gatattgacg tcgacccctg tgatcacatc accgctgacg
     3781 cgattggcaa acctggtcaa cgcgtttttt atctgcatgc ttattccgag catcgcacct
     3841 acactatcat cattgaaaaa gcgcagttgc tttcacttgc cattggcgtg gagcaattcc
     3901 ttggtcagct tagccagcaa taccctgacc ttgaagaagc ttctggcgat tatgttgaag
     3961 aagtcatgcg catcaacccg cccgtcgacc cgctcttccg cgctggtgag atcggcttgg
     4021 gttacgataa agaccgcgac cgtgtagtcg tcttcgcaaa agaactcatc actgaagaac
     4081 aagaccccga agaagcgtcg cagatccgtt tttgggcgac ccgtacccaa ctgcgtcagc
     4141 tcgcccgttg gggacaggaa gtctctcaac gaggtcgccc catttgcccg caatgcggtc
     4201 aacccatgga ggcgggcggt cacttctgcc ccaagaaaaa cggacacctt cactaacgtt
     4261 taaacgtgaa aacgttcgaa cgcttaaacg tttcccatga ccgatatcaa aaccatcctc
     4321 actcatggca actacgagct tcgtggtcag ttcatgttgg gctcgaatta caccttcctt
     4381 gtagatgttc accacgagga tgaagttgtt aaagccgtat acaaacccag caaaggcgaa
     4441 caaccgcttt gggattttcc cgacaacacc cttgcgttgc gcgaagtcgc tgcatatgaa
     4501 ctaagcgaaa cgcttggctt acacatcgta ccagtcaccg tctatcgcga cgatggtccg
     4561 catggacccg gctctttaca gcaatacatc gaatacgata tcgaatatca ctacttcaac
     4621 ttcaccgtgg aagataagca aaaacttcgt cccgttgtct tcttcgatat tctcgccaac
     4681 aatgctgacc gcaaaggaag ccatgtcttc tttgaggatg aaacccatcg cctctatgcc
     4741 atcgaccatg gcatttgttt tcatgaggag aataaactcc gcaccgtatt atgggatttt
     4801 gccggtcaag ccattcctga cgaattgctc gcgcctctct cgttgaccgg aaactggtct
     4861 gccctctttg agccttatct cagcccgcgc gagatcgatg ccctactctt tcgcgcagaa
     4921 gaattgtgcg attcaaaagt ctttccacgc ccgttacaag gacgaagggc gtatccttac
     4981 ccgcccattt aacgtcattg cgagggcgct cttgtttttt gcccgaagca atctaataac
     5041 gtgttgaaga ttgcttcgtt gggaagaaca ccttcctcgc aatgacggaa tacagtttca
     5101 ggagaaattt ttatgcacta caacatcgca ctcatcggtt ttggaaatgt cgcccgttcc
     5161 ttggctcgtc tgctggttcg caagcaggat gtcttgaagt cgaaatatga tgtcaccttt
     5221 tcatttactg gcatttcaac cggtcgtcac ggatatgccg tcaatcaaag tgggttggat
     5281 attcaaaaag ccttggactt ggtcgaaagc ggaaaagata tctctgagct ttctgtattc
     5341 cctgtcaatg actcgatcag cgtgatcaac aactctgctg ccaatgtgat gtttgaaaat
     5401 tctcccgtca atacccagac cggtcaacct gccgtggacc acatccgcac ggcgttgaat
     5461 ttgggaatgc atgctatcac cgcaaataaa ggtccagttg tgcatggcta tcgcgagttg
     5521 accgcgttag cggaatcgaa gggcaaaacg ttccggcacg agtctgctgt tttgggaggc
     5581 gcacccgttt tctcggtcat gcgtgaggcg tttccgctcg cggatcttga atccttcaaa
     5641 ggtatcttca atgctacgac caatgtcatc ttatctcgca tggaaagcgg cgagacgtat
     5701 gaacaggcac ttgcgtacgc acagaagatc ggtctcgctg aaaccgaccc caccaacgac
     5761 gtggatggct gggacgccgc tatcaaagtt gcgtcgcttg tcacagtgtt atgggacaca
     5821 cccatgaccc cacaacaagt gaatcccact ggcattcgtg gcattaccag tgagatgatc
     5881 gccaacgcca agaaagaagg caaacgctat aagttggttt gctcagctga aaggacggat
     5941 ggcaagattg ttgccagtgt ttcaccgcag ttggtcgatt catcttcgtc tctgtacggc
     6001 atgatgaact ctagcactgg catcaccttc cgcacagatg tgattattga ttactccatt
     6061 gtcctctctg aaaaacccgg tatgacgggt ggacccgtgg aaaccgcata cggtctcttt
     6121 gccgattttg tgaatatcgt ccgctaactg cccttttatt cgaatatcat tgttaagtag
     6181 taaagtagag cctttgtgtt tacaatagta gacacaaagg cttttatgac ccctccaaac
     6241 atcttgtata gtacggcact cttaacggct gggatttgtt gctttattgt tggtttattg
     6301 gtgttgcaaa gacgcccgtt tatgccgggg tcgatcccat tgatggtttt catccttgcc
     6361 atgtgctggt gggacatttc gtatggcatt ttctggggcg gcgttttaaa gccttcacct
     6421 tacttttggt tggatattag ttatatcggg gtggttatcg tcccggcggc gttttttgtt
     6481 tttgccatgc aaatctcagg caggcaagaa tggttgaaaa caccattgtt gattgggttg
     6541 tatgtagaac cgttgttggt tcttgttctg atgtggaccg acccctggca taatcttttc
     6601 tttgctggca aacggttgga aaacaccggc atgatcttaa atgcaggacc ggtattttgg
     6661 gcaaacgttg tatattctta tctgttgtta ttggtcacgt tcattttgtt agtgcgtaga
     6721 tttacaacga cttctggaat ttatcgccgc cagcttgggg ttgtgattgc aggtgcagga
     6781 gttacctggc tcaatagttt tatttttgtc actggtttaa gccctttccc aaatgcagat
     6841 aatacgcctt tttcatttac gatcgctgcc tttgcgtttg cctacgcttt gctccgttat
     6901 cgcatgttgg atattgtgcc cgtggcgcgc gatacattga tcgaaggtat gagcgacggt
     6961 gtgcttgttt tggatacaaa caatcgtatc gtggatatga atcccgaagc ggaaaaactt
     7021 ctctccgcct cttccgccgg gacgattggc aggcacgtag aagaagtttt cgccccgtgg
     7081 gctgaccttg taaaaacatt tatgaacgtg gaccaaaccc gggtggaagt tctagccagc
     7141 cgtgactcag atacatatct tgaccttcaa atttccccat tgttcgataa acgaaaaaac
     7201 tttattgggc gtttggtaat gtggcgtgat atttcacgct tgaagaaaat ccaaaccgag
     7261 cttgaaaaac ttgccacaca agacccgctg acccacgtct acaaccgacg tcatttttac
     7321 accttggcag aagtggaaat tcaccgctct ctccgttctc agttgccctt ctctgtcatt
     7381 ttggtggaca ttgattactt caagagcatt aacgataaat acggacataa agccggcgat
     7441 gaagcactaa tccactttac gaagatctgc atacagaacc tccgcaaagt ggatatattt
     7501 gccagatttg gcggcgaaga atttgtcatc ttgctgcccg aagcggactt ggatcaagcc
     7561 aaagagacag ccgagcgaat tcgaaagatc gtggaaagca cagtgctgaa cattgatgaa
     7621 catcaaatca agatcactgc cagttttggt gtttccacct ttaaaggcag ccccgatacc
     7681 ttagaccttt tgctgcaaaa atcagatgaa gctttgtatg ctgcaaaaaa tacaggacgc
     7741 aatcgggtcg tctgcgcaga ctaacttatg caactaacaa aatcaccaag gctacaatag
     7801 cggggacagc ctgaatgtaa aagatgcgct tgctgacggt gaatgcgccg aagattcctg
     7861 cgatgatgac acaactgagg aagaagattt tcaccgggtt gccagcgaat aatccccaca
     7921 gcaaaccggc ggcgaggaag ccattgtata gtccctgatt catggcgaga cgtttcgatt
     7981 gcttggcgaa ctcttcggtg agattgaaag atttcatgcc gcggggtttg tcccatagga
     8041 acatttcgag gtagaggaag aagaagtgca agagggcgac gataacaata agaatgtctg
     8101 atacgagttt cataaagttc tccttgggcg aatgaggtga ttttcacctg tgagtatagc
     8161 agaccctgaa aacagaacaa aaattctgtt atactgacca tcatgatttg tagggacgag
     8221 tcgcgactcg tccctacaag aggtatctaa tggcaaaaac aaaaactcat acccgatatg
     8281 tctgtcagca atgcagtcga gtctctgctt cgtacatggg caaatgcccg cagtgcgg
//
LOCUS       DAOQNJ010000041         8276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4380_length_8276_cov_10.152536, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000041 DAOQNJ010000000
VERSION     DAOQNJ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4380_length_8276_cov_10.152536"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..300)
                     /locus_tag="PLG52_02255"
     CDS             complement(73..300)
                     /locus_tag="PLG52_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40291.1"
                     /translation="MNVEHPERLIVLGVAMMLFGCIAPFLMVVKVWESTFFLNFLSYG
                     MSVLGMAIGFTGMAVMRVKQKRSDDDEDKYR"
     gene            complement(297..1286)
                     /locus_tag="PLG52_02260"
     CDS             complement(297..1286)
                     /locus_tag="PLG52_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40292.1"
                     /translation="MTDTQRDVLLSINDLHVWFELRRLGFGHAGYVKAVDGVTFDLHY
                     GETIAIVGESGCGKSSLMKTILGLNKPTKGSITFDGKQDVGTLEGEMLRQYRAKIGYV
                     QQDPFGALAPFMNVQRILEEPLIINGIKDPAERERRVRKVMEEVKLTPVDDFLHKFPH
                     MMSGGQQQRVVIARAIILEPKLVVADEPVSMLDASVRVEILKLLRGLQEKYKLAVIYI
                     THDLSTVRYFSERVFVMYAGNVVEKAPVDSLVHNPKHPYTTALMDATSDPDANNALTY
                     RELPSGEPPSLVNPPSGCRFHPRCPKRIENLCEVEEPSDLKSDDHLVACWLYK"
     gene            complement(1325..2287)
                     /locus_tag="PLG52_02265"
     CDS             complement(1325..2287)
                     /locus_tag="PLG52_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40293.1"
                     /translation="MANNDNLLRLEDLVLHFGTTRGSVQAVDKVNFSLNANQAVVVLG
                     ESGCGKSSLAKAILRLLPRNVVSYSGRVYLNGNDVMQLDDEEFRQNIRWASISMVPQA
                     AMNSLNPVIRVGDQVAEPAIIHLGVSKQEAVSLVKKMFQHVGVPEDFITRYPFELSGG
                     MRQRVAIAMALVTSPSLILLDEPTSALDVLTQASIFNVLKRLKGELGISFILITHDIA
                     TSSELADSVAVMYAGQIVETSDAHRFFAEPLHPYSRMLMASVPRLRANTEPEFITGQP
                     PSLLEPPKGCRFADRCPKRFDKCSEEPPVVNVDGIQVKCWLYTS"
     gene            complement(2288..3631)
                     /locus_tag="PLG52_02270"
     CDS             complement(2288..3631)
                     /locus_tag="PLG52_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40294.1"
                     /translation="MNNFAKSFRELLRYPSAVIGLITIIFLLVVSAYALITIPYGEAT
                     RLWRGGEDVWYQNPKYAAPAWMNLFSRVKQPVSFAVNTADGSMTKTVEVGAQDTSTIT
                     VVYSFDYAYDEPPQEMLLYFDSTFKDKLPFASVAWITPSGERIRVGDFAVDREMTFRI
                     SQDQKLMKRLKTDDVLPALFMEPKSDSTDLVKGTYQLEIIATTFEPDSDINVEFVLHG
                     KLFGLAGTDHYRRDLMLPLLWGTPIALTFGLLAALGTSLLTMIIAAVGSWYGGWVDEL
                     IQRITEINLVLPFLAILIMVGTFYSRSIWVILGVTILLSIFTGAIKSYRAVFVQSKVS
                     PYIEAARAYGTTDRRIIFSYLIPRMIPLLIPALVSSVPSYVFLEASLAVLGLGDPVLP
                     TWGKIIENAFAEGALYRGLYYWVLEPAALLMLTGLGFAMLGFALDRIFNPRLRDV"
     gene            complement(3628..4764)
                     /locus_tag="PLG52_02275"
     CDS             complement(3628..4764)
                     /locus_tag="PLG52_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40295.1"
                     /translation="MATETITATTGQVKKESLANTTIGRVLRYALVRLVTLAITVVIG
                     IYLTILIANMGGYVDTIMRNEIRDNTTQSILANPAFRQLTPEAREKLIQEKIANEEKR
                     LGLDKPFINRSFTYLVNGLTLNLGRAVNMNSDSGSRQVRLILLERLPATLLLAGISDL
                     ALFFGSVFLALGLSRKYGSFFDKLTVALSPTSAAPPWFYGIFLILIFAGVLHVLPFGG
                     LVDSPPPATSFGYAMSVLKHIILPASSIIVSSFFISVYNWRTFFLIYSSEDYVEMARA
                     KGLPSRDIERRYILRPTLPTIITNFALLIIGMWTGFTITETVFLWPGLGRTLFQAIGL
                     YDTPVIVGSTIIYAYLLGITVFILDFVYALVDPRVKVGGDNSQQ"
     gene            complement(5032..7569)
                     /locus_tag="PLG52_02280"
     CDS             complement(5032..7569)
                     /locus_tag="PLG52_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB40296.1"
                     /translation="MLRNRMFALLGLLVIASMVLAACGAPAATTEEAAPATEAAAPAT
                     EAATAEPTPVPTTRHGGWLDEIAYSSVTADSAVTQITAGAIDIYTRGLASDRVQEIKD
                     AGVCYAQSYGGYYDILFNPAVFTDTAKLNPFSNQKLREAMNWAIDRNYINQEIYGGGS
                     LAKYTVFTTQLVDYTGVIETARGLESYYAYNFDQAKAVVDAEMPAMGAELVDGKWAFN
                     GEPIVLAFLIRNDGDGTRLKIGDYFSDQLEKLGFTVDRQYRKSSEASPIWIGSDPKEG
                     QWNLYTAGWVSSGLTRDEKTGFQDMYLPSSSQGIPLFLENASDPAFVEAGDALANGAF
                     STLEERRALIEKALPLSLKDSLQVWIVDQQVYSPYQCNVEATYDLGSGLEAAQMPPYN
                     IRFKDQEGGQLKVGTNDLFTDPWNPVGGSNWVWDGFVQKATWSGSLMADPYTGLYWPL
                     RAESADVTVQTGLPVITNTDWVTLNTADTIEVPADAWSDWDATAQTFVPAGEGVTAKA
                     KIVMTYPADFFEVTKWHDGSNISVGDFVMRMIMTFDQAKPESAIYDEDSVPSYEAYKS
                     TFKAVKIVSTDPLVIETYTDRFYSDAELIATAGDWWPQYGFGEASWHAIAMANAAEAA
                     GETAYGTGKADRNAVEWTNFIGGPSLDLLNGQLDTLAAASTVPYAATLGQYVTADEAA
                     ARYANLKAFFEANGHFWSQTGPYYLASVDLNAKTALVKNNADFPDLSDRWASWSNPPL
                     ADATLDGPAQVKIGEATDFALTVTNKAGDAYPSSDIKQVKFLIYNDKGETIYVGEGVA
                     GAGDGEYTLTIPADVQLQAGAGKIEAAVVLYPAAIPAFSALEFVAAP"
     gene            7999..>8276
                     /gene="mscL"
                     /locus_tag="PLG52_02285"
     CDS             7999..>8276
                     /gene="mscL"
                     /locus_tag="PLG52_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNB40297.1"
                     /translation="MIMLKEFKEFVMRGNVLDLAVGVIIGGAFGKIVGSLVNDVIMPA
                     IGLLMGGVNFSELTIAGVIKYGAFIQTIIDFLIVAFVIFLIVKSANKM"
BASE COUNT         2422 a         1957 c         1954 g         1943 t
ORIGIN      
        1 attataacaa agaaaagaag cctgcaattg ccgttcagct tttgcaggct tcttcacgat
       61 caaaaaatgg atttagcggt acttgtcttc atcatcgtca gaacgttttt gtttgacgcg
      121 cataaccgcc atacctgtaa aaccaattgc cattccgagc accgacatgc cgtaggaaag
      181 gaagttcaaa aagaacgttg actcccaaac ttttacaacc atgaggaatg gcgcgataca
      241 tccaaatagc atcatcgcta ctccaagaac tatgaggcgc tcaggatgtt cgacgttcat
      301 ttatacaacc aacaagctac aaggtgatca tctgacttta aatcactagg ttcttccact
      361 tcgcagaggt tttcaatgcg tttggggcaa cgaggatgga aacgacaacc agaaggagga
      421 tttacaaggc taggcggttc accagaagga agctcacggt atgtcaaagc attgttcgca
      481 tccgggtctg atgttgcatc catgagcgca gtggtatatg gatgttttgg attgtgaacc
      541 aatgaatcaa caggcgcctt ctccacaacg tttccagcgt acattacgaa aacacgctcc
      601 gagaagtatc gaacagttga aaggtcatgg gtgatgtaga tcaccgccag tttgtatttt
      661 tcctgcaagc cgcgtaaaag cttgagaatt tcaacgcgga cggaagcatc gagcatggaa
      721 acaggttcgt ctgcaacgac cagctttggt tcaagaatga tcgcgcgggc aatcacaaca
      781 cgctgttgtt gcccaccact catcatgtga gggaatttat gcaaaaagtc atccaccggg
      841 gtgagtttta cttcttccat caccttacga actcggcgct cacgctctgc aggatctttg
      901 atcccgttga tgatgagcgg ttcttcaaga atgcgttgga cattcatgaa tggagccaag
      961 gcaccaaatg ggtcctgttg gacgtagcca atcttcgcgc gatattggcg aagcatttcg
     1021 ccttccaaag ttccaacatc ttgctttcca tcaaacgtaa tggagccttt ggttggtttg
     1081 ttcaaaccaa gaatggtctt catcaagctg gatttaccac aacctgactc gcccacaata
     1141 gcaatcgttt cgccatagtg caagtcgaag gtaacaccat caactgcctt cacataaccg
     1201 gcgtgtccaa aacccaagcg acgtaattcg aaccacacgt gcaggtcatt tatcgagaga
     1261 agcacatctc tttgtgtatc tgtcatgtct tgtagacctc tttttcgatt ttatcttaca
     1321 caaactagga tgtgtataac caacatttga cctgaatgcc atctacattt acaacaggcg
     1381 gctcttcaga acacttgtca aaacgtttag ggcaacgatc cgcaaaacga caacctttgg
     1441 gaggttccaa caagctgggg ggttgtccgg taataaactc tggttctgta ttggcacgta
     1501 aacgtggaac acttgccatc aacatgcgag aatacgggtg gagcggttct gcgaaaaagc
     1561 gatgagcatc gctcgtttct acaatttgcc ctgcatacat caccgcaaca ctatcggcaa
     1621 gttcactcga tgtagcaata tcgtgcgtaa taagaataaa gctaataccc agttcgcctt
     1681 tgaggcgttt tagcacatta aagatgctgg cttgggtcaa tacatccaac gcggaggtgg
     1741 gttcatccaa taggatcaag cttggagaag taacaagcgc catcgcaatg gcaacacgtt
     1801 gacgcatacc accgctcaat tcaaagggat agcgggtgat aaagtcttcg ggcacgccca
     1861 catgctggaa cattttcttt accagcgaaa cagcttcttg tttgctaacc cctaaatgga
     1921 tgatggcagg ttctgccacc tgatcaccca cacgaataac agggttcaag gagttcatcg
     1981 ccgcttgcgg gaccatggaa atggatgccc aacggatgtt ttggcgaaac tcttcatcgt
     2041 ccaactgcat cacatcgttt ccattcaagt aaacgcgtcc agaataagaa acaacattac
     2101 gaggaagaag gcgaagaata gccttggcga gcgagctttt tccgcagccc gactctccta
     2161 aaacaacaac tgcctgatta gcattcagag aaaaatttac tttatccaca gcttgaacag
     2221 agccgcgggt cgtgccaaaa tgtaaaacca aatcctcaag tcgaagaagg ttatcgttgt
     2281 tcgccattta tacatccctc aatcttggat tgaaaatacg gtcaagtgca aagcctaaca
     2341 tagcaaaacc caacccggtt aacatcaaca aagcagcagg ctctaacacc caataataca
     2401 aaccacgata gagggcgcct tctgcaaagg cgttttcaat aatcttgccc catgtgggca
     2461 aaacagggtc acccaaacca agaacagcca aggatgcttc caaaaatacg taagaaggta
     2521 cagaggaaac caaggcagga attagcaagg ggatcatgcg agggatgaga tagctgaaaa
     2581 tgatgcgtct atctgtcgtg ccgtatgcac gtgcagcctc gatataaggt gaaactttgg
     2641 attgtacgaa cacagcacgg tatgatttga ttgcaccagt gaagatacta agcaagatag
     2701 tcacaccgag aatgacccaa atactacgag agtagaacgt acctaccatg atcagaatag
     2761 caaggaaggg caaaacaagg ttaatctcag taatacgttg gatcaactca tcgacccaac
     2821 cgccatacca agaaccaacg gcggcaatga tcattgtcaa aagagaggtg cccaatgcag
     2881 cgagaagccc aaaggtcaga gcaattgggg ttccccaaag caaaggcaac attaggtcac
     2941 gacgataatg gtcggtgcca gcaagaccaa ataacttacc atgtaaaaca aattcgacgt
     3001 ttatatcaga atctggttcg aaggtagttg ctatgatctc aagttgataa gtacccttca
     3061 ccaaatcggt gctatcactc ttcggctcca tgaacagagc cggcaagaca tcgtctgtct
     3121 ttaatctctt catcagcttt tggtcttgag aaatacggaa tgtcatttcg cggtcaacag
     3181 caaaatcgcc aacacgaatt ctctcaccag aaggagtgat ccatgcaacg gaagcaaatg
     3241 gcaacttatc tttgaatgtg ctatcaaagt aaaggagcat ttcttgaggc ggttcgtcgt
     3301 atgcatagtc aaacgaatag acaaccgtaa ttgtggatgt atcttgagca ccaacttcaa
     3361 cggtcttggt catactacca tcagccgtgt tcacagcaaa cgaaacgggc tgtttcacgc
     3421 ggctaaataa gttcatccac gcaggggctg catatttggg gttctgatac caaacatctt
     3481 ctcccccacg ccataaacga gtcgcttcgc cgtatggaat tgtgatcagc gcataagctg
     3541 aaacgaccaa caaaaatatg atcgttatca aaccaataac ggctgaggga taacgaagca
     3601 gttcacggaa ggatttggca aaattgttca ttgttggcta ttatctccac ccaccttcac
     3661 gcgggggtca accaaggcat aaacaaagtc taagataaac actgtaatgc ctaacaaata
     3721 agcataaatg attgttgaac caacaatcac aggcgtatca tacaagccga ttgcctggaa
     3781 gagagttcgt cccaagcctg gccacaggaa gacggtttcc gtaatggtaa aacctgtcca
     3841 catgccgatg atgagcaatg caaagtttgt aatgattgta ggcaaagttg ggcgaaggat
     3901 ataacggcgt tcaatgtcac gagaaggaag accttttgct cgtgccattt ctacgtagtc
     3961 ttcactggaa tagattaaga agaatgtacg ccagttataa acacttatga agaaggaact
     4021 gacaatgatc gatgaagccg gcagaatgat gtgcttaagg acactcattg catacccaaa
     4081 agaggtggca ggcggaggtg agtccactaa cccaccgaat gggaggacat ggagtacacc
     4141 tgcaaagatc aagatcagaa agatgccata aaaccaaggt ggagcggcag aggtcggcga
     4201 taaagccacg gtcaatttat caaagaaact gccgtattta cgagacagcc ctaacgctag
     4261 gaatacgcta ccgaagaaca gtgccaagtc agagatacct gcaagcaaca gagtagcagg
     4321 caggcgttct aataagatca aacggacctg acgcgaaccg ctatcgctat tcatgttcac
     4381 tgcacgtcct aggttaaggg tcagcccatt caccaaatag gtgaaagatc gattaataaa
     4441 gggcttatcc aaacccagac gtttttcttc attggcaatc ttttcttgaa tcaacttctc
     4501 gcgtgcttcc ggagtcagct ggcgaaatgc tgggttggca agaatagatt gtgttgtatt
     4561 atcacggatt tcattccgca taatcgtatc tacatagccg cccatattag cgattaagat
     4621 cgtaaggtaa ataccaatta ccacagtaat cgcaagagtc accaacctga ccaaggcata
     4681 acgcaatact cgtccgatcg tagtatttgc gagtgattct tttttaacct gtccggtcgt
     4741 ggctgtaatt gtctcggtag ccatatcgca cctcaattca ttctttaatt gtgacctttt
     4801 tacaagcaat aaaaaatgaa gtcttgtatc tggtcatgta attcttttaa taattcaaaa
     4861 ataattccat caaaacatat tttcaaaata actattcaat ataaacggag tttatctttt
     4921 tgctaaggaa actttgttaa agtttttccc cgccactact aagaatacac cgtttacagg
     4981 aaaaaccagg ggcaaggttt cccttgcccc tggcaatttc aagaaaaaaa cttacggagc
     5041 cgcaacaaac tcgagtgcgg agaaggcagg aatggcagcc gggtaaagca caacggcagc
     5101 ttcaatcttg cctgcaccag cctggagttg aacatcggca ggaatggtga gggtgtattc
     5161 accatcgcca gctccggcaa cgccttcacc gacgtaaatg gtttcgccct tgtcattgta
     5221 gatcaagaac ttaacctgtt tgatatcgga ggaaggatag gcatcaccag ccttgttggt
     5281 cacggtcaaa gcaaagtcag tggcttcacc gatcttaacc tgagcaggac catccaatgt
     5341 ggcgtcagcc aaaggaggat tgctccaaga agcccaacgg tcagagagat ctgggaagtc
     5401 ggcgttgttc ttgaccaaag cagtcttggc gttgaggtca accgaagcga ggtagtaagg
     5461 accagtctgg ctccagaagt gaccattggc ttcgaagaaa gccttcaggt tggcgtagcg
     5521 agcagcagct tcgtcagcag ttacgtactg accgagggta gcagcatacg gaacggtgga
     5581 ggcagcagcc aaggtgtcga gctgaccatt caagaggtcg aggctcggac caccaatgaa
     5641 gttggtccat tcaacagcgt tgcgatcagc cttaccggtg ccgtaagcgg tttcaccagc
     5701 agcttcagca gcgttcgcca tagcaatggc atgccaggaa gcttcaccga agccgtactg
     5761 aggccaccaa tcgccagcag tggcgatcaa ttcagcgtca gaatagaagc gatcggtgta
     5821 ggtttcgatc acgagcgggt cggtcgaaac gatcttcaca gccttgaagg tgctcttgta
     5881 agcttcgtag ctaggaacag agtcttcgtc gtaaatggcg ctttcgggct tggcctgatc
     5941 gaaggtcatg atcatgcgca tcacaaagtc gccaacagaa atgttgctgc catcatgcca
     6001 cttggtaact tcgaagaagt cagcagggta ggtcatgacg atcttggctt tggcggttac
     6061 gccttcacca gcaggaacga aggtctgcgc agtggcatcc cagtcggacc aagcatcagc
     6121 aggaacttca atggtgtcag cagtgttgag ggtgacccag tccgtattgg tgataacggg
     6181 caaaccagtc tgaacggtca catcagcgct ttcagcgcgg agaggccagt acaaaccagt
     6241 atagggatca gccatcaagc taccactcca ggtggccttc tgaacgaaac catcccaaac
     6301 ccagttagaa ccaccgacag ggttccaagg atcggtgaac aggtcgtttg taccaacctt
     6361 gagttgacca ccttcttgat ccttgaagcg gatgttgtaa ggaggcatct gagcagcttc
     6421 gagaccggag ccgaggtcat aggtcgcctc aacattacac tggtaaggtg agtaaacctg
     6481 ctgatcgacg atccaaacct gcaaggagtc tttcaaggaa agcgggaggg ctttttcgat
     6541 caaagcgcgg cgttcttcaa gagtggagaa ggcgccattg gcgagagcat caccagcttc
     6601 aacaaaagcg gggtcagaag cattttcgag gaacagagga ataccctggc tgctggaggg
     6661 gaggtacata tcctggaagc cagtcttttc atcgcgggtg agaccagagg aaacccaacc
     6721 agcggtgtaa agattccact gaccttcctt aggatcagaa ccgatccaga ttggggaagc
     6781 ttcagaggat ttgcggtatt ggcgatcaac agtgaagcca agcttttcga gctggtcaga
     6841 gaagtaatca ccgatcttca agcgggtacc atcaccatcg ttgcggatga ggaaggcgag
     6901 aacaatgggt tcaccattga aagcccactt gccatctacg agctcagcac ccattgcagg
     6961 catttcagca tcaacaacag ccttggcctg atcaaagtta taagcatagt aggattcgag
     7021 accgcgggcg gtttcgatca caccggtgta gtcaaccaac tgagtggtga acacggtgta
     7081 cttggccaaa gaaccaccac cataaatttc ctggttgatg tagttgcggt cgatagccca
     7141 gttcatcgct tcgcgcagtt tctggttgct gaaggggttc agtttggcgg tatcggtaaa
     7201 gacagccggg ttgaagagga tgtcatagta accaccatag gactgagcgt agcagacgcc
     7261 agcgtccttg atttcctgaa cgcggtcaga agccaaacca cgggtgtaga tatcaatcgc
     7321 accagcggtg atctgagtaa cagcagaatc agcggttaca gaagagtagg caatttcatc
     7381 caaccaacca ccgtgacgag tggtaggaac aggagtgggt tctgcggtag ccgcttcggt
     7441 agcaggagct gctgcttcag tggcaggggc tgcttcttca gtggttgcgg caggagcgcc
     7501 acaggccgca aggaccatgc tagcgattac caacaaacca agtaaagcaa acattcggtt
     7561 tcgtaacatt tgttttctcc tccaagaaga aaaaattagg ggtttaacgt acaggcacaa
     7621 tacaaacaca cgttacgttt acgatcttag cgcatcacct ccttcttaat tttttaagag
     7681 gctgttacaa acttaaaaaa gaatcacaag gaattatacc taattcttta ggctagtcaa
     7741 caggggtgct gcggaagagg aatgtatagt cacatttcag cccgagtcta atatgcagaa
     7801 aggaaaaatt gaccctacta gaggactgtt ttcagaaaat attaataaaa ctttgtatga
     7861 caattcgaca aaaagccccg cctcaaacca gacccgaaac ctgtttcaac ccatttagca
     7921 tttctaccca accgatgcct ttcgttgaca atccccctcc cctgccatac aattcattta
     7981 tcatttcatc aaggagatat catcatgtta aaggaattca aagaatttgt catgcgcgga
     8041 aatgtactcg accttgcagt cggtgtcatc atcggcggcg cattcggcaa gatcgttggc
     8101 tctctcgtta atgacgttat catgcctgcc attggtttgc ttatgggggg tgtcaatttc
     8161 agcgaactga caattgccgg tgtcatcaaa tacggcgctt tcatccaaac catcattgac
     8221 ttcctcattg tagccttcgt tatcttcctt atagtaaagt ctgccaacaa gatgaa
//
LOCUS       DAOQNJ010000042         8228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4420_length_8228_cov_7.256087, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000042 DAOQNJ010000000
VERSION     DAOQNJ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4420_length_8228_cov_7.256087"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1430
                     /locus_tag="PLG52_02290"
     CDS             60..1430
                     /locus_tag="PLG52_02290"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNB40298.1"
                     /translation="MTDSQKAVEYVRSNSERFLNELKDLVAIPSISTLDENKSDVQRA
                     AEWVAGQLRSIGMDNVKIMPTALHPVVYGEWMGAGKSAPTVMIYGHYDVQPVDPLELW
                     TSDPFKAEVRGDYLFARGASDMKGQVVASIKAVEAIMKTEGKMPVNIKWLVEGEEEIG
                     SEHLGDFIKNNKELLAADFCLNPDAGMIGPDKPTITTGLRGLAYFELRVFGPDKDLHS
                     GLFGGTVHNPAQALIELIAGMHDKNGKITLPGFYDKVRKLSKKEREDFKRLPTTNKDL
                     IKMTGVPALWGEPQFIPAERVGARPTLEVNGLLSGFTGQGSKTVLPAWAMAKISCRLV
                     PDQTPEQTEKQLRAYLKKHAPKTIKWELKNLHNAGSALTEVDSAGVKALAKALETVWG
                     KRPYFKREGGSIGSVVLLQKYVGADSLLTGFGLPDDNVHSPNERLHLPTWYKGIEAFT
                     HFFYNF"
     gene            1524..>2167
                     /locus_tag="PLG52_02295"
     CDS             1524..>2167
                     /locus_tag="PLG52_02295"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNB40299.1"
                     /translation="MEDRIITYGGQAVIEGVMMRGQKAFAVAMRAPDGKIVTHTEKLA
                     EVYRSGLTKVPFLRGVILLWDALGLGMRALTLSANTQTGEDEKLEGPALYLTLGLSLT
                     MGIGLFFLLPAGIGGWVEHAMAQTTSALWIGNLIEGVLRLLILIGYIWSIGFMPDIRR
                     VFQYHGAEHKTINAFEAGAELTPEVVAKYPIEHPRCGTAFLLTLVLLSILVFTL"
     assembly_gap    2168..2267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2268..3225
                     /locus_tag="PLG52_02300"
     CDS             <2268..3225
                     /locus_tag="PLG52_02300"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNB40300.1"
                     /translation="VEKETGFRPSLFIFSSSWLDSIFHMRAWIKSTWFWLGIVALFFI
                     AVGVYVISMLGRPPAFPTKYELYPGVTYHRVVRYFPHAMIAHVVVIDRKEAKASFLVT
                     PPDQVEGGTVKARTTSEFLKEFGVQIAINGDGFSPWWSRSPVDYYPHQGDPVTPLGFA
                     AAFGKIYSDGLQQPTQEPTLYISRRNELSFNRAPGKLQHAISGDRMLLLSGEVVPDLD
                     DTTLQPRTAIGTNRNGRYVILVVVDGRQPFYSTGATFKELAELMKAYDAFFAMALDGG
                     GSSTLVVQKKNGGTQILNSPIDNYIPGRERPVANHIGVSIAP"
     gene            3498..4745
                     /locus_tag="PLG52_02305"
     CDS             3498..4745
                     /locus_tag="PLG52_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNB40301.1"
                     /translation="MKFKNLFRVAMLFATIISVFGTGAQAYAQGNSTNYLGAQIIIRS
                     TTFWDAVFNGTVNANRYERWSLQLIEPENFTISIITTSGTLTPDIYLLDASENQIASA
                     TGTLSSASLTTNQLAGDYFIQVQPHSGDGTYLMEITRTEQVVEPTASVVLNPAVIPVG
                     GTSTGTLMLSNIPSGGYASAEFTCTYNPALVQISNITDAGLFGSDPAVAVNGPVDGSF
                     VFAVAGSNSQRATSDGAVFTFTVTGLAAGQADIACTVRVSAGGSLSTITSTPASLTIE
                     EVTATLNGTVNATKTVTVTLYKQDTTVEATTTVDTSGNFTITAPAGTYTVVASAPGFL
                     KAQGSPVLTAGATKTMQTISLLAGDIDGNDVIDQFDALTIGMNYNLIEPTAADLNNDG
                     TINVLDLELLAANYRASGASAWQ"
     gene            4808..5470
                     /locus_tag="PLG52_02310"
     CDS             4808..5470
                     /locus_tag="PLG52_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HNB40302.1"
                     /translation="MKKFALTLIALICLFVVMPVKAQSAESIWIAASTNSFKTGETVI
                     VTVNGLSATPIQGLTFQIKFDPACLQPVNAASPIAGMNGLSLPQISGFVDASFASTTP
                     QVANGVLAEVRFITLGACQTNLTLESAALVIKNESGFAAPLAGVNLGEKAVALAISAE
                     KGTSADAPLLGTPLALGTEPENSGSSLPAGTTIILAVIGVLLIVGIVVLVRILRGENS
                     RG"
     gene            5510..8119
                     /locus_tag="PLG52_02315"
     CDS             5510..8119
                     /locus_tag="PLG52_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB40303.1"
                     /translation="MKAKTSTRLFLILAIFLVAVPTSVFASTAPPVDMFQLPWEQGKA
                     WYPYDGLDNGLKRSSTSPHNYNMGGALDFAPRAKMTVGEDTSNDWVTAAAAGTVYQVG
                     SCYVKIDHGNGWITEYWHLDKIQVTTGTKVSRNQRLAIIHNNKTQQVCIGNEYPGPHL
                     HFVFRPKVLDTRFAGWTVNYNLFTNITTFSKSGQTVGRLQPLMNIPDLQIANRGILDW
                     NINYEGNIDAYRQERWSLMLVDEAKFEINVTPKGIGLVPVIVLLDASGNEITRASGTL
                     STTQPAGFYYVDVRSEAGTGYYNIIATKSESGGSTVTPSPTASETGTFTPTVTATLNG
                     SETPTATGTLTGSETPTATGTLTGSETPTATETPIGLATHTPTSTGGPDTSTPEPSTD
                     TPTPTLTATATFTPTSTPDFEQTAIAATQTSNAATLTAIANEATQTAISASETANAAT
                     LTAIANGATQTAIAATQTFDAQTQTAIAGTLTATADAATQTAIVGTQTAIAGTLTATA
                     DAATQTSIAITQTANVGTQTAIAATQTSDAAMQTAIAGTLTAQPGIQTSIAGTATANA
                     LTMTASASTFTPSPTGTATVVPTPVGPYVLVDVIQPTLTLNQTSLVNVSLNNVPAEGY
                     TSTEFVCTYDPAVIEVSNILAAELFGSDPASALSGPQNGQFILAIAGSNGKKATSSGT
                     ALTFSVRGLQVGQSTVQCTARVSMGQGNLEPIAYIPDVVNVIDVSVTPTPTTGGVTGQ
                     VIASKPVTIRLYDAQNTLVISQVANPDGTFNLQVPAGTYTIIASADGFLNAQGFLTIT
                     NGGIVTKPTINLPAGDIDGNSVIDQFDALTIGMNYSGSLPAAADLNNDGVINVLDLEL
                     LAANYRLSGALSW"
BASE COUNT         1967 a         2137 c         1967 g         2057 t
ORIGIN      
        1 aacagaccct aaaggcattc aaagccttta gggtcattca tactataagg agtttcatca
       61 tgactgattc tcagaaagct gttgaatacg tccgctcgaa tagtgaaaga tttttgaacg
      121 agttgaagga tttggtcgcc attccttcga tctctacttt ggatgagaac aaatcagatg
      181 tacagcgcgc cgccgagtgg gtggcgggtc aactgcgctc catcggcatg gacaatgtga
      241 agatcatgcc gaccgctttg catcccgtcg tctatggcga atggatgggg gcgggcaaat
      301 cggctcccac tgtgatgatc tatggacatt atgacgtcca acccgtcgac ccgctagagc
      361 tttggacgtc tgacccgttc aaggcggagg tgcgaggcga ttatttgttc gcacgcggcg
      421 cttcggatat gaagggtcaa gttgtggcat ccatcaaagc ggtggaagcc atcatgaaaa
      481 ccgaaggcaa aatgccggtg aacatcaaat ggttggtgga aggcgaagaa gaaattggct
      541 ccgaacatct tggcgatttc atcaagaaca ataaagaact gcttgccgct gatttttgtt
      601 tgaaccccga tgccggcatg atcggacccg ataagcccac catcacaacc ggtttgcgcg
      661 gtctcgctta ttttgaatta cgcgtctttg gtcccgataa agatttgcac tccggcttgt
      721 ttggcggcac cgtccacaac ccggctcaag cgttgatcga actcattgcc ggcatgcacg
      781 ataagaatgg caagatcacg ttgccaggct tttacgacaa ggtccgcaag ttgagcaaga
      841 aagaacgtga agatttcaaa cgcctgccca ccaccaacaa agacctcatc aaaatgaccg
      901 gcgtgcctgc cctgtggggt gaaccccaat tcatccctgc cgaacgcgtt ggcgctcgcc
      961 ctactcttga agtgaacggc ttgctctctg gttttaccgg tcagggttcc aagaccgttt
     1021 tgcctgcctg ggcaatggcg aaaatttcct gccgtctcgt acccgatcaa acgcctgaac
     1081 aaaccgagaa acaattgcgc gcctatctca agaaacatgc gcccaagacc atcaaatggg
     1141 aactgaagaa tctgcacaat gctggctccg cattaaccga agttgactct gctggtgtga
     1201 aggcgcttgc caaggcgctc gaaaccgtgt ggggcaaacg accttacttc aagcgcgaag
     1261 gcggttctat tggctcggtt gtcttactgc aaaaatacgt cggtgccgat tcgctcctca
     1321 ccggtttcgg cttgcccgat gacaacgtcc actcgccgaa tgagcgtttg catttaccca
     1381 cttggtataa gggcattgaa gcctttaccc atttctttta caacttctaa ataacaagca
     1441 agggcgaccc gtctggcgag atcaatcact ttgatccaca tgggcgggtc gctcttgtat
     1501 atcgaaagaa agaaacgaga attatggaag atcgaattat tacatacggc ggtcaagccg
     1561 tgatcgaagg ggtgatgatg cgcgggcaaa aagccttcgc cgttgccatg cgcgcgcccg
     1621 atggcaagat cgtcacacac actgaaaagc tggcagaagt ttatcgctct ggtcttacca
     1681 aagtcccttt tttgcgcggt gtgattttat tatgggatgc actcggactc ggtatgcgtg
     1741 ctttgactct ttctgccaac acccaaaccg gcgaggatga aaagctggaa ggaccggctc
     1801 tttatctgac cttgggcttg tctctcacca tgggcattgg actcttcttt ttgcttcccg
     1861 ccggaatcgg cggctgggtg gagcatgcca tggcgcaaac cacctccgcg ctttggatcg
     1921 ggaacttgat cgaaggcgtg ttacgtttgt tgatcttgat cgggtatatc tggagcattg
     1981 gtttcatgcc cgatattcgc cgtgtctttc aatatcatgg cgccgaacac aaaaccatca
     2041 acgcctttga agccggcgct gagttgactc cggaagtggt ggcgaaatat cccattgagc
     2101 atccgcgctg tggcaccgcc tttttgttga ctttggtctt gctctccatt ctcgtgttca
     2161 ccttgttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt agagaaagag
     2281 acgggttttc gcccgtctct ttttattttt tcttcatctt ggcttgatag cattttccac
     2341 atgagagcat ggataaaatc tacgtggttt tggctgggaa tagtagcgtt gttcttcatc
     2401 gctgtagggg tatatgttat ctccatgttg gggcgccccc cggctttccc aacaaagtat
     2461 gagctttacc ccggtgtgac ttatcatcgc gtggtgcgtt attttccaca tgccatgatc
     2521 gcgcatgtgg ttgtgattga tcggaaagag gcgaaggcaa gttttctggt cacgccgcca
     2581 gaccaggtcg agggtggaac cgtaaaggcg cgtaccactt ctgagttttt gaaagagttc
     2641 ggggtgcaga tcgccatcaa tggcgatggc ttctctccgt ggtggtctcg cagcccggtg
     2701 gattattatc cgcatcaggg agacccagtc acacccttgg gatttgctgc cgcgtttgga
     2761 aaaatctact cagatggttt gcagcaacct acgcaagagc caacgctata catcagccgt
     2821 cgtaatgaat tatcgtttaa ccgtgcgccg gggaaattgc aacatgccat ttcaggagac
     2881 cgcatgttgc ttcttagtgg agaggttgtg ccagatttgg atgatacaac tttacagcca
     2941 cgcacagcca tcggtacgaa tcgcaatggg cgttatgtga ttctcgtagt ggtggatggt
     3001 cgccagccgt tttatagtac tggtgccaca ttcaaagaac tggctgagtt gatgaaagca
     3061 tatgatgcct tctttgccat ggcattagat gggggtgggt cttctacatt ggttgtgcaa
     3121 aagaaaaatg gtggaacgca aatattgaat tcacctattg acaactatat tccggggcgt
     3181 gaacgtcctg ttgcaaatca tattggtgtc tcgattgcac cgtaattaaa aatagatttt
     3241 taaattgatt tttcaagacc cacttcacat gaagtgggtc ttgattctta agcagacatg
     3301 tcgcaactat tctgcaatat cgtggcaact cacctgcaac gcatattgtg cagttgcacc
     3361 aatagaatgt aattggcttt taagtcttca tcattgaacc gccacggttg gcagtttgga
     3421 ggaaatacga aacgataccc ttttttgtta tgtaggcttt ctgccttata tcaccttgat
     3481 tcgaaaagga gaatacgatg aaatttaaga atttgtttcg cgttgcgatg ctgttcgcaa
     3541 ccatcatcag tgtctttggc acgggtgcgc aagcgtacgc gcaaggaaac agcaccaatt
     3601 atcttggcgc acagatcatt attcgcagca ccaccttttg ggacgcagtc ttcaatggga
     3661 cggtaaatgc gaaccgctat gagcgctggt cattgcagtt gatcgagcct gagaacttca
     3721 ccatctcgat catcaccaca tctggcacgc tcactcccga tatctacttg ttagatgcca
     3781 gcgagaacca gatcgcttct gcaacgggta cgctttcaag cgcttcccta accaccaatc
     3841 aacttgcagg ggattacttc attcaagtcc agccgcactc tggggatggt acttatttaa
     3901 tggagatcac ccgcactgag caggtggttg aaccgaccgc atccgtggtg ttgaacccgg
     3961 cggttattcc tgttggcgga acctccacag gtactttgat gttgagcaat atccccagtg
     4021 gcggatatgc cagcgcagaa tttacttgta cctataaccc cgctttggtt cagatcagca
     4081 atattaccga cgctggtttg ttcggttctg accccgccgt tgccgttaat ggacccgtcg
     4141 atggttcctt cgtgtttgcg gtagcaggca gcaatagcca gcgtgctact tcagatggcg
     4201 ctgtcttcac ctttacggtg acaggtcttg cagctgggca agcggatatt gcttgtaccg
     4261 tccgtgtttc agctggcggt tcgttgagca ccattacgtc caccccggct tcgttgacca
     4321 tcgaagaagt gactgccact ctcaatggta cggtcaacgc caccaagact gtgacggtga
     4381 ctctctataa gcaggatacg actgtagaag ccaccactac tgtggacaca agcggaaatt
     4441 ttacgatcac ggctccagcg gggacttaca ccgttgtcgc atcggctccc gggttcctca
     4501 aagcccaggg cagcccggtc ctcaccgctg gtgcaaccaa gaccatgcag acgatctctc
     4561 ttctcgccgg tgatatcgat ggcaatgatg tgatcgatca gtttgatgct cttacgattg
     4621 gtatgaacta caaccttatt gaacccaccg ctgcagactt gaacaatgat ggcaccatca
     4681 atgttcttga ccttgaatta ctggcagcga actaccgcgc ttctggcgca agtgcctggc
     4741 aataaatccc ctggcaatgc aaagggtgca ccgattcatc atcggtgcac cctccatgga
     4801 gacttcaatg aaaaaatttg cactgaccct cattgcactt atctgtcttt ttgttgttat
     4861 gcctgtaaag gctcaatctg cagaatccat ttggattgct gccagcacga acagttttaa
     4921 aactggtgaa acagtgatcg ttacggtcaa tgggctttct gccaccccga ttcaaggtct
     4981 gacctttcag atcaagtttg acccggcatg ccttcaacct gtcaacgctg ccagccctat
     5041 tgctggcatg aacgggcttt cccttccaca gatcagcggg ttcgtggatg cttcctttgc
     5101 aagtaccacg cctcaagttg cgaatggcgt gttagctgaa gtacgtttta ttactcttgg
     5161 tgcctgtcag accaatttaa cgttagagag tgctgcgctc gttattaaga atgaatctgg
     5221 gtttgcggct cctttggctg gtgtaaacct tggagaaaaa gccgtggctc tcgccatcag
     5281 cgctgagaag ggtacctctg ccgatgcgcc tttgttgggc actccactgg cgttaggcac
     5341 cgaacctgag aattccggtt cttctctacc tgccggcacc accatcattc ttgctgtgat
     5401 tggtgtgctt ctcatcgtcg gtattgttgt gcttgtccgc attttgcgcg gtgaaaattc
     5461 gcgcggttaa ttttatccac agtccgtttt gatagggcac ggtgccatta tgaaagctaa
     5521 gacatctacc agactatttt tgatcctcgc gatctttctt gttgctgttc caacaagtgt
     5581 atttgcgagc acggcacctc ccgtggatat gttccaactg ccttgggaac aaggcaaagc
     5641 ttggtatcca tacgatggac tggataatgg actcaagcgt tcttctacca gcccacataa
     5701 ctacaatatg ggcggcgcgt tagactttgc tcctcgtgca aagatgacgg taggggaaga
     5761 tacctcaaat gactgggtca ctgccgcggc agctggaacg gtctatcaag tcggttcttg
     5821 ttatgtcaag atcgatcatg gcaatggctg gatcaccgaa tattggcact tggacaagat
     5881 tcaagtgacc actggcacca aggtgagccg aaatcaacgc ttggcgatta ttcacaataa
     5941 caaaacccaa caagtctgta ttgggaatga gtatccgggt ccacatttgc attttgtatt
     6001 ccgacccaaa gtactcgata cgcgctttgc cggttggacg gtcaactaca acttgttcac
     6061 gaatatcacc accttctcaa agagcgggca gacggtgggc aggcttcagc ctttgatgaa
     6121 cattcctgac ctgcagatcg cgaatcgtgg cattcttgat tggaatatca attacgaagg
     6181 caatatcgat gcatatcggc aggaacgttg gtctcttatg ctggtcgatg aagcaaagtt
     6241 tgagattaac gtcaccccga aaggaattgg gcttgtcccg gtcattgttt tgttagacgc
     6301 aagtggaaac gagattacac gggcgagtgg tacattgagc accactcagc ctgctggttt
     6361 ctattacgtc gatgtccgtt ctgaggcggg cacgggatac tacaatatta ttgccaccaa
     6421 aagcgaatca ggcggttcca ccgtcacgcc atcaccgacg gcttcagaaa cagggacatt
     6481 cactccaacc gtaactgcaa ccttgaacgg ctcagagacc ccaaccgcaa ccggaacctt
     6541 aaccggctct gaaactccaa ctgcgacggg tacgctcact ggttctgaaa cccctacggc
     6601 tactgagacc ccaattggtt tggcgactca taccccaact tctactggtg gtccagatac
     6661 aagcacgcct gaacccagca cagatactcc cacgccaact ctaacggcta ctgctacatt
     6721 tacaccaact tctacacccg attttgagca gacagcgatt gcggcgacgc aaacatcaaa
     6781 tgcagccacg ttaactgcca ttgcgaacga agcaacccaa accgcaattt cagccagcga
     6841 aacagcaaac gccgcaacgc taacggcaat tgccaatggt gccactcaaa cagctattgc
     6901 agcaacacaa acttttgatg cccaaactca aaccgccatt gcagggacgc tcacagccac
     6961 agccgatgcc gctacacaaa ctgccattgt cgggactcaa accgcgattg ccggaacact
     7021 cacagcaact gcggacgcag ctacgcaaac ttctatcgct ataactcaaa ctgcaaatgt
     7081 gggcacccag actgctattg ccgctacaca aacttctgat gcagccatgc aaactgccat
     7141 cgcaggcacg ttaacggctc agcctggaat tcaaacttcg atcgcgggta ccgccacagc
     7201 caatgcactg acaatgactg catctgcgtc cacctttaca ccttcgccga caggtacggc
     7261 gacggttgta cccacccctg tgggacccta tgtgttggta gatgtcattc aaccgaccct
     7321 cacacttaac caaaccagtc ttgtcaatgt cagcttgaac aatgtccctg ccgaaggata
     7381 taccagtaca gagtttgttt gtacttacga ccctgcggtc attgaagtca gcaatatcct
     7441 cgctgcagaa ctcttcggtt cagaccccgc ttctgctttg agcggacctc aaaatggaca
     7501 gttcatcctt gcgattgcag gcagtaacgg caagaaagcc acctccagcg gcaccgctct
     7561 aaccttctcg gtacgcggat tgcaagtagg tcaaagcact gttcaatgta cagctcgcgt
     7621 ttcgatgggg cagggcaacc ttgagccaat tgcttacatt ccagatgtcg tcaacgttat
     7681 tgatgtgagc gttaccccca caccaacgac aggcggcgtg actgggcaag tgattgctag
     7741 caagccagtc accatccgtt tgtatgatgc tcaaaacaca cttgtcatct cgcaggttgc
     7801 caacccagat ggaaccttca acttacaagt ccctgctggc acctatacca tcatagcttc
     7861 tgctgatggc ttcctgaatg cccaaggttt tctaacaatc accaacggag gcatcgtcac
     7921 caagccaacc atcaacctgc cagctggtga tattgacggc aacagcgtga tcgaccagtt
     7981 cgatgcactc acgatcggga tgaactacag cggctctctc cccgccgcag ccgacctcaa
     8041 caacgacggc gtaatcaacg tcctcgacct tgagctactg gcagcgaatt atcgattgtc
     8101 tggcgcgctt tcgtggtaat aaaaaaatgg cgacccatcg ggtcgccatt tttattttaa
     8161 aattcgatct ccccgatctg ttcaaagtta atgtcgaaca actcttcggc ttctgcctct
     8221 aattccac
//
LOCUS       DAOQNJ010000043         8189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4458_length_8189_cov_8.664741, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000043 DAOQNJ010000000
VERSION     DAOQNJ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8189
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4458_length_8189_cov_8.664741"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PLG52_02320"
     CDS             complement(<1..1165)
                     /locus_tag="PLG52_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNB40304.1"
                     /translation="MSTTSYPFARESQLALDQARRIAEGNKLPFVDSLMLLLTLLQLK
                     NSRVQALLKSLNVRVDNLTARIVATIKLEAKEILQVSEGKPVLSSECETVLALASEEM
                     QLAGQSSISEFSLLLGMLKNTESKAGQILSQYAVVVDQAREKIKVFKETPVSQSNILR
                     KDGFLRRAFRNGISPIFISLVLFTIAMAAFLWLGIGNNPRAFLIAFIIGGWIISVALH
                     EFGHAITAFWGGDESVEDKGYLTLNPLKYSNPVLSVIIPVMMLLLGGIGFPGGAVYIN
                     IFALRKPKYRSLVSAAGPLANLLVAILLAIPFANFPFFFFFIYAPQKFLMALAFLAFL
                     QITSIIINLLPIPGLDGFGILEPFLPREIVGVASFIRPFGFLILYLFVFIDSPI"
     gene            1226..1918
                     /locus_tag="PLG52_02325"
     CDS             1226..1918
                     /locus_tag="PLG52_02325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNB40305.1"
                     /translation="MTQLTGQERAQYVQSMFSKIAHRYDLMNRLMTGGQDIRWRREVI
                     SLTKIRPHGSILDLGTGTGDLAREALTQYPKAQVTAADFTLEMMRVGKKNGDLNFSTA
                     DALNLPFKGKNFDAVVSGFLMRNVTDVQQALREQHRVLKPGGRIVILDTTRPKKNLLS
                     PFIKFHMHVIIPMVGGMLSGMRDAYEYLPDTTENFLTAEELSVRMVAVGFRRVNFKRL
                     MLGTIAIHWGEK"
     gene            1918..2394
                     /locus_tag="PLG52_02330"
     CDS             1918..2394
                     /locus_tag="PLG52_02330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNB40306.1"
                     /translation="MSKIKQPNSRHCFICGVENPVGLHLHFYETEPGVIETEYTAPDH
                     FEGFPGVLHGGIVAAIIDEVSGRAYMGNDPANPRFMFTGKLEIKYRKNVPIGKLLRIV
                     GKVMENKGRIAKAWGGIYDAETNELLAEGTGMHINVPSDQFDMSKLEELGWKIYPD"
     gene            2499..3071
                     /locus_tag="PLG52_02335"
     CDS             2499..3071
                     /locus_tag="PLG52_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB40307.1"
                     /translation="MTTLMNMEIKELDEEDLARKAISDADAFAELYRQHVTRVYRYHM
                     AHIGNVKDAEDLTSQTFMAAMESIRSFRGDGSFAAWVMGIASKKRLMYFRGNRHEVPL
                     EAAEHYPNPSLPTDKAAMQRIRLQSISHALKQISSERSEAIVLIYFGGLSYAEAAGVM
                     RKNEASVKMLVSRGLQDLRERTSLRMEVEE"
     gene            3068..5566
                     /locus_tag="PLG52_02340"
     CDS             3068..5566
                     /locus_tag="PLG52_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNB40308.1"
                     /translation="MNEFDKNKKLETLLEETAEQLQPNVMFKAELEEKLRKAHKPRAS
                     LPTFAFRNWLPTLTGIVVVGGLVIFMLWLFQTLEPQKSVGTNEEQPIEVQTTPGVIID
                     PNNTNSNETIGGYDYRGGKLFLTSALPESSATANIYEYIADQPVTIEEARALANQFGL
                     QGEMYTTAFPQAPDKIGYVISDGKRMLTVYTKNYFTYTPDILVTDRGYYGVNNDNAET
                     VISDFFNQNGLTFPFSISKSPAKGMYHFMQLSPDSIPMQYDIYTIQSSNVTLGQNGEV
                     AYLSANMLSYNPQAFGSYGIISAEDALNAILDPHSTVGMTESGSGGEGGVIPQQWYRE
                     YPNNETVSISGSLIKYDSATAGQPPLIFVGSFEALGNTTDLQALSNFAFVQVTGQFVE
                     ENGIRKFNVESTNANVIQGYITGSLRKEGDQIILTSDDGNSEYVLLDPPADLPLNTVP
                     TESYLGISGILVDNTLDWSNIQYVADASSMGGGGGGGGFGFYPLNLSGTPAVFPTPIP
                     AEEQYSAAEVASFLNYIVENGDTLESIAAKFNVSLEDLMRVNYISDMNVIETGWLIKI
                     PGVPGPTQLTGEEGIVSISKYIKPDGRVRYQYTFLSSQSYNGIYYELQGDNLEPLQNL
                     VNRPVKIWGDISYSDTGTMFLDVKQFEEIYPGLQFQILQGKEALTEINGKPVALFTTN
                     GTTYVMLSEVGAYQDISYKGDFPEDINIEALIVPGETYEGMPALRVFSMANAKDPNTG
                     EAIELEPFAGEIQSSPDPFGNSDVYVEPDFIVDYIQLEYYVTDPKMLYDAPTTDTLYI
                     QPVWHFSGHYTNGTVSDILVQALKQEYLYPFNSP"
     gene            5641..6243
                     /locus_tag="PLG52_02345"
     CDS             5641..6243
                     /locus_tag="PLG52_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNB40309.1"
                     /translation="MDQLLLILTYIAVIASSVSGALGARKYQMDIVGAVTIAYVNAFG
                     GGTVRDLLLGRTPIFWIADPALTVTTFIVAIISFYRLDRISKLLLTTADAIGLGIFSI
                     LGATYTLQLNLSPIVAIHMGVVTGIFGGVLRDVLSNRIPKVFGQSTELYATCSFAGTT
                     VFIILYVLHVNTLLASLAGALTIFTMRLLAVRFKMTLPSP"
     gene            6277..7017
                     /locus_tag="PLG52_02350"
     CDS             6277..7017
                     /locus_tag="PLG52_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNB40310.1"
                     /translation="MISTYHRPQTLDEALTLLTQPNTVPLGGGTLLSKATADSVAVVD
                     LQRLGLDALRVNGNELQIGATCTLQSLLESANCPASLKTAIKLEAPLNIRNTATIAGT
                     LVASDGRSPFTAMLLAMDAKLDYRVLTNQSSIGLSEFLLTHPSGLVTSITIPLNTKTA
                     FEFVSKTPADKPIVGAAVAQWNSGRTRLTLCGYSKTPMLAMDGTEADGIQEAARNAYH
                     EANDEWASAEYRMDVAAVLAGRALAALT"
     gene            7021..7287
                     /locus_tag="PLG52_02355"
     CDS             7021..7287
                     /locus_tag="PLG52_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40311.1"
                     /translation="MQTCPICNKEVQPSQRYPNYVCAECADLAVSKDGRKLIFYNQSI
                     SGGYEAKYADTGEEYNSHDCYINGIKCHANEHHFGGIVIIKVES"
     gene            7287..7562
                     /locus_tag="PLG52_02360"
     CDS             7287..7562
                     /locus_tag="PLG52_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40312.1"
                     /translation="MTKEKIKAFEEIARIFEISNESAKYFLNHVQKSFKVERPPHKLI
                     LEFVEKQNYEFKPTPYDVALAMKESGVWDYDLGSPPPALEDEEDLYQ"
     gene            7680..8165
                     /locus_tag="PLG52_02365"
     CDS             7680..8165
                     /locus_tag="PLG52_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNB40313.1"
                     /translation="MEFQSGDWVVHCAHGLGQITAIETRSFGEQTIPYYQVQIGDLTI
                     WVPTDEHLGKRLRKPASQSDFSNLLNMLTNPPETLPSDRRQRNQYLIELLKDGTAETL
                     CKVIRDLSAIRKHRTWSEYDRDILRRVQKSLIGEWSFIFSINPHDAENELQKLLAKHI
                     A"
BASE COUNT         2357 a         2033 c         1809 g         1990 t
ORIGIN      
        1 aaatcggcga atcgatgaac acaaagagat acaaaatcaa aaagccaaac gggcgaataa
       61 aactggcaac accaacgatt tctcgtggta agaatggctc aagaataccg aagccatcca
      121 agccggggat gggcaataaa ttgatgatga tagacgtaat ctgcaaaaac gccagaaaag
      181 ccagtgccat caaaaacttt tgaggggcat aaataaagaa gaaaaagaat gggaagttag
      241 cgaatgggat cgctaacaat atagcgacca ataaatttgc taaaggtcca gccgcagaaa
      301 ctaggctgcg gtattttggt ttgcgcaatg cgaagatatt gatatacact gctccacccg
      361 gaaaaccaat cccacctaat agcaacatca taactggaat gataacgctc aacaccgggt
      421 tcgaatattt aagcgggttg agggtgagat agcctttatc ttcaacactt tcatcgccac
      481 cccaaaaggc tgtgatggcg tgcccaaact catgcaatgc cacagagata atccacccgc
      541 cgataatgaa cgcaatcaag aaagctctgg gattattccc aatgcccagc cacaagaaag
      601 ccgccatggc aatcgtaaac aacaccaaac taataaagat cgggctaatg ccatttcgaa
      661 acgctctgcg cagaaaccca tctttgcgca aaatattact ttgtgaaaca ggggtttctt
      721 tgaatacctt aattttctca cgggcttggt ctactacaac cgcatattga gataatattt
      781 gcccggcttt gctttctgtg ttttttaaca tgcccaacaa aaggctaaac tcactgatcg
      841 agctttgacc tgccagttgc atctcttcag aagccaatgc taaaacagtt tcacattcag
      901 aagataagac cggtttcccc tcagaaactt gaaggatctc tttcgcctcc agtttgatag
      961 ttgccacaat gcgtgcggta agattatcca ctctgacatt gagagatttc aacaaggctt
     1021 gcacacgtga atttttgagt tgcaacagag taagcaaaag catcaaagaa tctacaaaag
     1081 gaagtttatt cccctctgca atgcggcgag cttggtcaag cgcaagctgg ctctcccggg
     1141 caaatggata cgatgtagtt gacattttgc gattataccg tgcgtcaaaa accttctgtg
     1201 tataattcgt ttttcaggag aatttatgac ccaattaacc gggcaggaac gtgcccagta
     1261 tgtacaatcc atgttcagta agatcgccca tcgctacgat ctgatgaacc gactgatgac
     1321 aggcgggcaa gacatccgtt ggcggagaga agtcatcagc ctgacgaaga tccgcccgca
     1381 cggctctatt ctggacctcg gaacgggtac cggtgattta gctcgcgaag cgctgacaca
     1441 gtaccccaaa gcccaagtca ccgcagcaga ttttacgctt gaaatgatgc gtgtcggcaa
     1501 aaagaatgga gatttgaact tctctactgc cgatgctttg aatttgccat tcaaaggaaa
     1561 aaacttcgac gcggttgtct ctggttttct catgcgcaat gtgaccgacg tccaacaagc
     1621 gttgagagag caacaccgtg tgctcaaacc gggtggacgt atcgtcattc ttgatacgac
     1681 acgcccaaag aaaaatctac tttcaccgtt catcaaattt catatgcatg tcatcatccc
     1741 catggtgggt ggaatgcttt ctggcatgcg cgatgcttat gaatatttac ctgatacaac
     1801 agaaaacttt ttgaccgccg aagaactgtc cgttcgtatg gtggctgttg gcttcagaag
     1861 agttaatttc aaacgcctga tgcttggcac aattgccatt cactggggag aaaaataatg
     1921 tctaaaatca aacaaccaaa ttcacgtcat tgctttattt gtggagttga aaaccctgtt
     1981 ggcttacacc tccattttta tgaaaccgaa ccgggagtca tcgaaaccga atacaccgcg
     2041 cccgaccatt ttgaaggctt ccccggtgtg ctgcatgggg gcatcgtcgc cgcgatcata
     2101 gacgaagtct ctgggcgggc atacatgggc aatgatccgg cgaatccgcg ttttatgttt
     2161 acgggcaagt tagagatcaa ataccgcaag aatgtgccga ttggcaaatt gctcaggatc
     2221 gttggtaaag taatggagaa taaggggcga attgccaaag cctggggcgg aatttacgat
     2281 gcagagacga atgaactgct tgccgaaggg acaggcatgc acatcaatgt gccaagtgac
     2341 cagttcgata tgagcaagct tgaagagtta ggttggaaga tataccctga ctagccaggt
     2401 gacagtcgct tataaagtga ctgtcacctt ttattttgcc tgttactttt ttcaacctcc
     2461 tgcaacatat ctatgtgcgc actactcaca ggaggtatat gaccacccta atgaatatgg
     2521 aaatcaaaga actagacgaa gaagacctag cacggaaagc catctcagat gcagatgctt
     2581 ttgcagagct ttaccgtcag catgtgacgc gggtataccg ctatcacatg gcacatatcg
     2641 gcaacgtaaa agatgccgaa gacttaacct cgcagacctt catggcggcc atggaaagta
     2701 tccgctcgtt tcgaggagat ggctcgtttg cggcgtgggt gatgggaatc gcttcaaaga
     2761 agagacttat gtattttcgc gggaacaggc acgaggtccc gttagaggcg gcggagcatt
     2821 atcccaaccc gagcttacct accgataaag ccgcaatgca acgcatccgg ctccagtcta
     2881 tttctcatgc gctcaagcaa atttcaagtg aacgctctga agcgatcgtg cttatctact
     2941 ttggcggatt aagctacgcc gaagccgcag gcgtgatgag aaaaaatgag gcgtctgtca
     3001 aaatgctcgt ctcacgcggg ctgcaagacc tgcgcgagcg aacctccctc agaatggagg
     3061 tggaagaatg aacgaatttg ataaaaacaa aaaactcgaa acgttattgg aagaaactgc
     3121 cgagcagcta caacccaatg taatgtttaa agccgaattg gaagagaagc ttcgtaaggc
     3181 acataagcca cgcgcaagtt tgccaacctt tgcatttcga aactggttgc ccacgttgac
     3241 aggtattgtc gttgttggcg gattggtaat tttcatgctt tggctcttcc aaacgttgga
     3301 gccacaaaaa agcgttggca ctaacgaaga acaacctatt gaagtgcaaa caactccggg
     3361 cgtcatcatt gacccgaata atacaaatag caacgaaacc attggcggat atgactatcg
     3421 cggcgggaag ttgttcctta catctgcttt gcctgagagt tcagcaactg ccaatatcta
     3481 tgagtacatt gctgatcagc ccgttaccat tgaagaggcg cgagcgcttg caaatcaatt
     3541 tgggttacaa ggtgaaatgt atacaaccgc atttccacaa gcgcccgata agattggtta
     3601 tgtcatttca gatggcaagc gaatgcttac tgtgtatacc aaaaattact tcacatacac
     3661 acccgatatt ttggtaacag accgcggtta ctatggtgtg aataacgaca atgcagagac
     3721 ggtcatttct gatttcttca atcagaacgg gttaacgttc ccattcagca tcagcaagtc
     3781 tcctgcaaag ggtatgtatc atttcatgca actttcgcca gatagcatcc ccatgcaata
     3841 tgacatctac accatccaaa gttcaaatgt cacgcttgga caaaatggtg aagtagctta
     3901 tctctctgca aatatgctca gctacaaccc acaagcattc ggaagctatg gcatcatcag
     3961 cgcggaagat gccttgaatg ccatcctcga cccacatagc accgtcggca tgaccgaaag
     4021 cggctctggc ggagaaggcg gcgtgattcc acaacaatgg tatcgagaat atccaaataa
     4081 tgagaccgtt tcaatctctg gcagcctcat aaaatacgac tctgcgacag caggtcagcc
     4141 accgttgatt tttgttggca gttttgaggc acttggaaac accacagact tacaagctct
     4201 gagcaacttt gcctttgtac aagtgacggg tcaatttgtt gaggaaaatg gcatccgcaa
     4261 atttaatgtg gaaagcacga acgccaatgt tattcaggga tatataaccg gctcgctgcg
     4321 caaggaaggc gatcagatca tccttaccag cgacgatggc aacagcgagt atgttttgct
     4381 agacccaccc gcagacttgc cccttaatac ggtgcctacg gagtcatatc tcggtatctc
     4441 tggaattttg gtcgataaca ctctggactg gtcaaacatc caatatgttg cagatgccag
     4501 tagcatgggc ggaggtggcg gaggcggcgg gtttggtttc tatccgctca acttaagcgg
     4561 cactcctgct gttttcccaa caccaattcc agcagaagaa caatatagcg ccgcagaagt
     4621 cgctagtttc ttgaactata ttgtagagaa tggtgacacc ttggaaagca ttgctgccaa
     4681 attcaacgtc tcgttggaag acctcatgcg cgtgaactac atttcagata tgaatgtcat
     4741 tgaaacaggc tggctcatca aaattccagg cgtaccaggt cctactcaac taacaggcga
     4801 agaaggtatt gtaagcatct cgaagtacat caaaccagat ggcagagtgc ggtatcaata
     4861 caccttctta agctcacagt cgtacaatgg catctattac gaattgcaag gcgataatct
     4921 tgagccactt caaaacttgg ttaaccgccc ggttaagatt tggggcgata tcagctactc
     4981 agatacaggg acgatgttct tggacgtaaa acagttcgaa gaaatctacc cggggttgca
     5041 attccagata ctgcaaggca aagaagcgct taccgagatc aatggaaaac ccgttgccct
     5101 ctttacaacc aatggcacca cctatgtgat gctctcagaa gtaggtgctt atcaagatat
     5161 tagttataag ggtgatttcc ctgaagatat caacattgaa gctctgatcg tgccgggtga
     5221 aacatacgaa ggcatgcccg ccttgagggt attcagtatg gcaaatgcca aagacccgaa
     5281 caccggagag gctattgaat tagaaccatt tgctggcgag atacaatcat ctccagatcc
     5341 attcgggaac tcagatgtat atgtcgaacc tgatttcatc gttgattaca tccaactcga
     5401 atactatgtt accgacccca agatgcttta cgacgctccc accacagaca cgttgtacat
     5461 tcaacccgtc tggcacttta gcggacatta cacaaacggc accgtctctg atattctcgt
     5521 ccaagcgctc aaacaagaat atctctaccc cttcaattca ccgtaaccag taaagcaaca
     5581 aaaaagtcca ccatcacatg atggtggact ttttaataca agttgtgtga tacttggcga
     5641 atggatcaac ttcttctcat tctcacttac atcgccgtca tcgcatcatc ggtttcagga
     5701 gcgctgggcg cacgtaaata tcaaatggac attgtaggcg cagtgaccat tgcctatgtc
     5761 aacgcctttg gcggcggtac cgtccgcgac ttattgctgg ggcgcacgcc catcttctgg
     5821 atcgccgacc ctgcattaac cgtcactacg ttcatcgtcg ctattatctc cttctatcgg
     5881 ctcgaccgta tctcaaaact actgctcacc acagccgatg ccattggtct cggcatcttt
     5941 agcatccttg gcgcaaccta caccctacaa ctcaatcttt ctcccatcgt tgccattcac
     6001 atgggcgtgg tcactggcat ctttggcggc gtcttacgtg acgtgttgag caaccgcatc
     6061 cccaaagtgt tcggtcagag taccgagctt tatgccactt gctctttcgc tggcacaaca
     6121 gtatttatca ttctttacgt tctgcatgtg aacaccttgc ttgcatcgct agcaggtgcg
     6181 ctcaccattt tcaccatgcg tctactcgca gtccgcttca aaatgaccct cccctctcca
     6241 taacgattta taatgcatat ctatggaggc acttctatga tctcgaccta tcacagacct
     6301 caaactctcg acgaagccct gacgcttctg actcaaccca ataccgttcc cctcggaggc
     6361 ggtacgcttc tttccaaggc gacagctgac tctgttgccg tagtcgatct ccaacgcctc
     6421 ggcttggatg cgctacgcgt caacgggaat gaactccaaa tcggtgcaac ctgtactcta
     6481 cagtctctgc tggaatcagc taattgcccc gcatcgctaa aaaccgcaat caaactcgaa
     6541 gctccgctca atattcgcaa cacagcgaca attgccggaa ctctcgtcgc cagtgacgga
     6601 cgttctcctt tcacagcgat gttattagcc atggatgcaa aactggacta tagagtatta
     6661 accaatcaat catctatcgg tctaagtgaa ttcctcctca cccatccaag cggactcgtc
     6721 acctccatta ccattccgct caataccaaa accgctttcg aattcgtttc caaaaccccg
     6781 gcagacaaac ccatcgttgg agctgccgtc gcacaatgga attcagggcg aacccgcctt
     6841 accctgtgtg gatacagcaa aactcccatg ctcgccatgg atggtaccga agcagatggc
     6901 attcaagaag ccgcacgcaa cgcctatcac gaagccaacg acgaatgggc ttctgccgaa
     6961 taccgtatgg atgttgcggc agtgctggca gggcgtgcat tggcagcact tacctagtct
     7021 atgcaaacct gtccaatctg caacaaagaa gtacaacctt cgcaacgtta ccccaattac
     7081 gtctgcgccg agtgtgctga cctcgcagta tcaaaagatg gcagaaaact aatcttctac
     7141 aaccagtcaa tttctggtgg gtatgaagcc aaatacgctg ataccggcga agaatataac
     7201 agccacgatt gttatattaa tgggattaag tgtcacgcca acgaacatca ctttggcggc
     7261 attgtgatca taaaagtaga aagctaatga ccaaagaaaa aattaaagcc tttgaagaaa
     7321 tcgcgcgtat ttttgagata tccaacgaaa gcgcaaagta ttttctcaac catgttcaga
     7381 aaagctttaa agttgaacgc ccgccccata aattgatcct tgaatttgtt gaaaaacaaa
     7441 attatgaatt caagccaact ccatacgatg ttgccttagc catgaaagaa agcggcgtat
     7501 gggattacga cctcggctct ccaccgcctg ccttggaaga cgaagaagat ctctatcaat
     7561 aaaagcagct agcatcggtg atataatcat ccctatataa cagattgaca gtgtctaagc
     7621 gccagtcctt ttgtcggact ggcgcttttc ttttcccatc aagtacaaag gagacttata
     7681 tggaatttca atcaggcgat tgggtcgtcc attgtgcgca tggcttgggg caaatcacag
     7741 cgattgaaac gcgttctttt ggggagcaaa ccattcccta ttatcaggtt caaattggcg
     7801 atctcactat ttgggttccc acagatgagc atttaggcaa acgcttgcgc aaacccgcaa
     7861 gccagtcaga ttttagtaat ctgctaaaca tgctaacgaa tccaccagag acactgccta
     7921 gtgaccgccg tcaacgcaac cagtatctca ttgaactcct caaagacggt accgccgaaa
     7981 ccttatgcaa ggttattcgt gacctatccg ccattcgcaa gcatcgcacg tggagcgaat
     8041 acgacagaga tattctgcgg cgcgtacaaa aatcgttgat cggtgagtgg agctttattt
     8101 tttcaatcaa cccacacgac gcagaaaacg aactgcaaaa attattagcc aaacatatag
     8161 cataaggaaa acatcatgca atcagaaga
//
LOCUS       DAOQNJ010000044         8090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4548_length_8090_cov_9.927194, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000044 DAOQNJ010000000
VERSION     DAOQNJ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4548_length_8090_cov_9.927194"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PLG52_02370"
     CDS             complement(<1..604)
                     /locus_tag="PLG52_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HNB40314.1"
                     /translation="MTKKLRVAVLFGGRSGEHDVSLMSARSVVSVLDPNRYDVIQVGI
                     TLDGNWLTGENALDAFEKGDTSDLDRVIPPTDPASRQSLHVLRDTKMGEKFETLAAID
                     VFFPVLHGPFGEDGTIQGLFEMADVAYVGAGVAGSSVGMDKGIFKDVMRANGIPQVPS
                     MLVLRGDIESKLDDVIKRAEELGDYPLFTKPANLGSSVGIS"
     gene            complement(607..1530)
                     /gene="murB"
                     /locus_tag="PLG52_02375"
     CDS             complement(607..1530)
                     /gene="murB"
                     /locus_tag="PLG52_02375"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNB40315.1"
                     /translation="MVDTTFAVDLLRQKFGDAVQENVTLAPYTSARIGGLADIMLSVK
                     SADELAEAMQLIWEHNFPYYILGGGSNVLVSDQGFRGVVVLNRAKETRFETGDQPTVW
                     CESGVIIANLAKRCASKGLAGLEWSATVPGTVGGAVYGNAGAFGGDMTHNLIWAELLT
                     KNGREKFGLEQMGYGYRTSVLKRGEAQGIVLSALLKLKNSSKEEVSVKIEQFSERRKA
                     TQPPGASMGSMFKNPEGDYAGRLIESAGLKGTRIGNAEISTVHGNFFINHGETKAEDI
                     LALIHVVQNTVKEKFNIQLELEVELVGDWNK"
     gene            complement(1542..2924)
                     /gene="murC"
                     /locus_tag="PLG52_02380"
     CDS             complement(1542..2924)
                     /gene="murC"
                     /locus_tag="PLG52_02380"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNB40316.1"
                     /translation="MTHVHFIGIGGSGLSAIARLLKESGYEVTGSDQSLSPFAAELQS
                     IGIQVFVGHHPRNVSGAEMVIRSSAIKDENPEVKAALQAGIPVYKRSDFLGRLMENKI
                     GIGIAGTHGKTTTTAMVAWMLYSLNRDPSFIVGGTLNNLKVNARAGKGDTFVIEADEY
                     DRMFLGLKPQIEVVTNLEHDHPDCFPTFADMYSAFESYIDLLPPDGTFITCAEDDGAA
                     TLISHARRKGLSVVSYSVQGEMTINSPQWVQARTMHPNEKGGFDFSVMTNMGSMTNAF
                     KVSLQVPGEHNVRNALAALCVAAVLGLPMQKAAEALSLFTGTGRRFEVRGEPKGVLLV
                     DDYAHHPTEIRATLAGARARYPERRIWAVWQPHTYSRTQTLQYEFSRAFNDANEVLVT
                     EIYASREAKQEFSSAEVVASMPHSSARYSGSIEETEKYLLEHVRFNDVVIVLSAGDAD
                     QITAALLKEL"
     gene            complement(2946..3494)
                     /locus_tag="PLG52_02385"
     CDS             complement(2946..3494)
                     /locus_tag="PLG52_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNB40317.1"
                     /translation="MMSAVYIFWMYVFLFGIIGAMRGWAKELMVIFSVVTSLAVNLLL
                     EKYIPLTRNLVDPNNPTQDALTAQFWIRTLILTALVYFGYQTVNISRFAPKAARERLQ
                     DSMFGAVLGGVNGYLVAGSVLYYNHVANYPFRNVISPATDAAIADAINRMMNVMPPRF
                     LAEPQIYFAVIIILIFIIVVYI"
     gene            complement(3491..4549)
                     /locus_tag="PLG52_02390"
     CDS             complement(3491..4549)
                     /locus_tag="PLG52_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNB40318.1"
                     /translation="MYPALSVYEALKSKHPNVETLWVGGEGGMEEDLVKRAGIPYRSV
                     PAAGVHGVGLRALPGNLAKLTRGVLESRRILKEFKPDVLFFTGGYVAGPMAVAGRNLP
                     IVLYVPDIEPGLALKFLGYFADVITVTAPDSKKFFSHPERIVHTGYPLRSGLLNWSRE
                     KAISHFRLDSSLPTLLVTGGSKGARSINMAVLKSLNELLEMTQIIHLTGSLDIETAQQ
                     AAANLSAEKKLRYHALPYLHDMGAALSAADLVLSRAGASSIGEYPFFGLPAVLTPYPY
                     AWRYQKVNADYLAERNAAIILQDELLNDNLLTVIKDVLNNAQKRQAMRTAMKNLSMPN
                     AADTIASQLVKLAGDETL"
     gene            complement(4509..5750)
                     /locus_tag="PLG52_02395"
     CDS             complement(4509..5750)
                     /locus_tag="PLG52_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNB40319.1"
                     /translation="MGIGTFVRETSPQDRPRAGGFDMPLLVIVVALIVFGLVMLYSAS
                     WDFSRAAYNGDAMYMFNRQLMWLGLGTVAALILAFIDYHRWRQFIVPAMAFTVLMLII
                     VLVMSEVRFNATRTIFNGSIQPSELAKLVSILYLAVWLYAKREMLKDITFGLIPLGVI
                     LGFVGGLIYLQPDLSAAATVLMLGGLLFFLAGGDLKQIGGLLIIAFAVAWLVVSISLT
                     GQQRVSDFVAGIKDPLQASYHVRRSFEAIVNGGWFGVGIGKSLSKVTGLPVPPTDSIY
                     AVVVEELGWFGGVGLIGLFGALVWRGLVIARRAPDMLGTLLASGLVIWIGLEALINMA
                     VMVGLLPFAGNALPFVSAGGSNLVTTLAALGILFNISRQGSSANETNDEWRSYSAVAN
                     LRWWNGRGRVSRSVRLRSAKE"
     gene            complement(5729..7102)
                     /gene="murD"
                     /locus_tag="PLG52_02400"
     CDS             complement(5729..7102)
                     /gene="murD"
                     /locus_tag="PLG52_02400"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNB40320.1"
                     /translation="MTNWTGKHVLILGAARQGLALARWLARHGANVTLNDSRREEDLI
                     TAKKSLADVNVTWAVGGHPLELLDTTDVLCISGGVPLTLPIVEEAIKRDIPLSNDSQI
                     FMEVVSCKTVGITGSAGKTTTTTLVGNMAKLAKGDRAFVGGNIGDPLINYVDDMQADD
                     LAILELSSFQLEQMTISPNIAAILNITPNHLDRHGTMEAYTNAKAHILDFQSAKDNAI
                     LGRDDKGAWGLKNRVKGNLLSFSLQDLDEGLNGTYLHDGLLSLRDGFAYVPLLMREKV
                     QLRGDHNIANVLAAFAIGHAAGFKLDDMLEAVEEFHGVPHRLELVRELNGARWYNDSI
                     ATAPERAAAAVHAFSEPIVLMLGGRDKNLPWGDLAKLIHERVDHVVLFGEAGEMIQNA
                     ITAYSGVGTVDVHRAMTLKEAVTLAADVTSAGDVVLLSPGGTSFDEFKDFAERGEAFR
                     KWVSELS"
     gene            complement(7104..>8090)
                     /gene="mraY"
                     /locus_tag="PLG52_02405"
     CDS             complement(7104..>8090)
                     /gene="mraY"
                     /locus_tag="PLG52_02405"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNB40321.1"
                     /translation="ALSLSLAGLAFIMTAIWGNPLLRILRHFKLGKIIRIEQPEGHRV
                     KMGTPTMGGVMFIVPVLLLSGLLNAVALIGVTASGVGRSVLLPMVVMVGFAILGAVDD
                     WEGIHGKRKGDGMRARTKFAFQVVLALATAYVLKYFIKAPDLYLPGYFFEIQIGWIYV
                     PIAAFIIVAESNAVNFTDGLDGLAGLIAATAIAAFGGIALMQEQIYLARFCFILVGAL
                     FGFLWFNVHPAQLIMGDTGSMALGSVLAVIALMTGQWPMLLVIAIIPLAEMMSVVIQV
                     TYFRVTGGKRFFKMTPIHHHFELLGWSETQIVQRFWLIGLMAALVGIGLSQV"
BASE COUNT         2234 a         2241 c         1838 g         1777 t
ORIGIN      
        1 tgctgatacc tacagaggaa ccgagattgg caggcttggt aaacaaggga taatcgccca
       61 attcttcggc tcttttaatg acatcatcca gcttgctttc aatatcaccg cgcaacacca
      121 acatggatgg cacctgcgga ataccgttgg cacgcatcac atctttgaag atgcctttat
      181 ccatgcctac agaagagccc gcaacccctg cgcccacata agcaacatct gccatttcga
      241 acagcccttg aatggtgcca tcctcgccga agggtccgtg cagaacgggg aagaacacat
      301 caatcgcggc aagggtttca aacttctcgc ccatttttgt atcacgcaag acatgtaaac
      361 tctggcgtga agccgggtcc gtaggaggga tgactcgatc taaatcgctg gtatcgccct
      421 tttcaaaagc atctaatgca ttctcaccgg tcaaccaatt gccatccaga gtaatgccta
      481 cttgaatcac atcatagcga ttcgggtcta acacagaaac gaccgagcgc gcagacatca
      541 gcgaaacatc atgttcgcct gaacgtccac caaataaaac tgccactcga agtttcttcg
      601 tcatcgttat ttgttccaat cgcctacaag ttcaacttcc aattccaact gtatgttgaa
      661 tttctctttt accgtgttct gaactacatg aatcaaagcc agaatatctt ctgctttggt
      721 ttcaccgtga ttgatgaaaa aatttccgtg caccgtgctg atctcagcgt tgccgatgcg
      781 agtccccttc aagcctgcag attcaatcaa tcgcccggca tagtcacctt cggggttctt
      841 gaacattgaa cccatactgg cgcccggcgg ctgtgtggct tttcggcgtt cactgaattg
      901 ctcgatttta acagacactt cctctttcga agaatttttt aacttcaata atgcacttaa
      961 cacaatccct tgcgcctcgc cgcgcttcaa aacactggtg cgatatccat agcccatttg
     1021 ttcaagcccg aacttctcac gtccattctt ggtgagcaac tctgcccaaa taaggttatg
     1081 ggtcatatcg ccgccaaatg cccccgcatt gccataaacc gctccgccca ctgtgccagg
     1141 gactgtggct gaccattcga gcccggcaag acccttggat gcgcagcgct tggcaagatt
     1201 tgcaatgata actcccgatt cacaccagac cgtgggctgg tcgccagttt caaaacgggt
     1261 ttccttcgcc cgattcaaaa ccaccacccc acgaaagcct tgatcgctca ccaacacatt
     1321 cgaaccgccg ccaagaatgt aatagggaaa gttatgttcc cagatcaact gcatcgcctc
     1381 tgccaactca tccgcagatt tgaccgaaag cataatgtca gcgagtccac caatgcgcgc
     1441 agacgtgtac ggcgcgagag tcacattctc ttgcaccgca tcgccaaact tctggcgcaa
     1501 caagtcaaca gcaaaggtgg tatccaccat caacatccta actagagttc cttcaacagc
     1561 gccgccgtga tctggtctgc atcgcccgca gagagcacaa tgaccacatc attaaagcgc
     1621 acatgttcta ataaatattt ctccgtctct tcaatagaac cgctataccg agccgaagaa
     1681 tgcggcatgg aagcgactac ttctgcagaa gaaaattcct gtttggcttc gcgtgaagcg
     1741 tagatttccg tcaccaacac ttcattggca tcgttgaatg cgcgtgaaaa ttcatattgc
     1801 aacgtttgag tccgtgaata agtgtgtggt tgccacaccg cccaaattcg gcgttcggga
     1861 taccgagcgc gagcccctgc taacgtagca cggatctcag tgggatgatg ggcataatca
     1921 tcaacaagca acacaccctt cggctccccc cggacctcaa agcggcgccc cgtccctgtg
     1981 aatagactca acgcctcagc cgccttttgc ataggaagcc ccagcactgc tgcaacacac
     2041 aacgccgcca aagcattgcg cacattatgt tcacccggca cctgcaaaga cactttgaaa
     2101 gcattggtca tgctgcccat attggtcatc actgaaaaat caaagccacc tttttcgttc
     2161 gggtgcatcg tacgagcttg cacccattgc gggctgttga tggtcatctc accttgaaca
     2221 gaatacgaaa ctacgctcaa gcctttacgg cgggcatgcg agatcaacgt cgcggcgccg
     2281 tcatcttctg cacaggtgat aaacgtgcca tctggcggca gaaggtcgat gtaactttcg
     2341 aaggcagagt acatgtctgc aaaggttggg aagcaatcag ggtgatcgtg ctccaaattg
     2401 gtaaccactt cgatctgtgg tttgagccca aggaacatgc ggtcatattc gtcagcttcg
     2461 atcacaaacg tgtcgccttt gcctgctcgc gcattgacct tgagattgtt caaggtgcca
     2521 cccacaataa aggaagggtc acgattgagc gaatacaaca tccatgcaac catggcggtt
     2581 gtagtagtct tgccgtgggt gccggcaata ccaataccga tcttgttctc catcaagcgc
     2641 ccaagaaaat cggagcgttt gtagacgggg atacctgcct gcaatgccgc cttgacctcc
     2701 ggattctcat ccttgatggc agaagagcgg atgaccattt ctgcgccgga aacattgcgt
     2761 gggtgatgcc ccacaaaaac ctgaatacca atactctgca attcagccgc aaaaggagaa
     2821 agggactggt ctgagccggt cacttcgtag ccgctctctt tgagcaggcg cgcgatagca
     2881 gaaagccccg aacctccaat gccgatgaaa tgaacatgag tcataaagta atccagcaga
     2941 aagtattaga tatacacaac aatgatgaag atcaaaataa tgatgaccgc aaaatagatc
     3001 tgcggttctg cgagaaagcg cggcggcatc acattcatca tacggttgat cgcatccgca
     3061 attgcggcat cggttgccgg gctgatcaca ttgcgaaagg gatagttcgc gacatggttg
     3121 taataaagaa cagagccagc caccaaatat ccgttcacac cgcccaagac cgcaccgaac
     3181 atggagtctt gtaagcgttc acgggctgcc ttcggggcaa agcgtgagat attcacggtt
     3241 tgatacccaa agtacaccaa cgccgtcagg atcaaggtgc gaatccaaaa ctgggcggtg
     3301 agtgcatctt gggttgggtt gttggggtct acaaggttgc gggtcaatgg aatgtatttt
     3361 tccaacaata agttaaccgc caacgaagtg accacgctga agatgaccat caattctttc
     3421 gcccatccgc gcatggcgcc gatgatgcca aagaggaaaa catacatcca gaatatataa
     3481 acagcactca tcataaggtt tcgtctcctg ccagttttac caactggctg gcaatggtgt
     3541 ctgccgcatt gggcatggat aaatttttca tcgctgtacg catggcttgg cgcttttgcg
     3601 cattgttcaa tacatcttta atgaccgtca agagattatc gttcaacaat tcgtcttgca
     3661 agataatcgc agcgtttctt tctgccaaat aatcagcatt gaccttctga tagcgccacg
     3721 catacggata gggcgtaagc acggcaggca acccaaagaa gggatactcc cctatcgaag
     3781 aggcaccagc tcgagacaag accaagtctg cggcggagag ggctgcaccc atatcatgca
     3841 aatagggcag ggcatgatag cggagctttt tctctgcaga taaattcgct gcggcttgtt
     3901 gtgcggtttc aatatccaac gagccagtga ggtgaatgat ctgcgtcatc tctagtaatt
     3961 cgttcaaaga tttcaacaca gccatgttga tcgaacgtgc gcctttgcta ccgccagtca
     4021 ccaagagggt gggaagggaa gaatccaacc ggaagtgaga aatggctttt tcgcgagacc
     4081 agttcaaaag tccggaacga agcgggtagc cggtgtggac gatgcgctct gggtgtgaga
     4141 aaaacttctt tgagtccggc gcggtcacgg tgatgacatc cgcaaaatag cctaggaatt
     4201 ttagcgcaag ccctggctca atatctggta cgtagagaac gatgggcaag ttgcgccccg
     4261 ctactgccat gggacccgca acatagccgc cggtgaaaaa caaaacatcg ggtttgaatt
     4321 cttttagaat acgacgtgat tcaagtacgc cgcgggtgag tttggcaagg ttgcctggca
     4381 atgcgcgcag acccacgcca tgcactccgg ccgcaggaac agaacggtat ggaatgcctg
     4441 cacgtttcac caggtcttct tccatgccgc cttcgccgcc aacccaaaga gtttcgacgt
     4501 ttggatgctt actctttagc gcttcgtaaa cggacagagc gggatacacg cccccgcccg
     4561 ttccaccagc gcaaattagc aaccgcactg tacgacctcc attcatcgtt cgtttcgttc
     4621 gcgctacttc cctgccggga aatgttgaag agaattccca acgccgccaa ggtggtgacc
     4681 aagttcgagc caccagcact gacgaacgga agagcgtttc ctgcaaacgg cagcagcccc
     4741 accatcactg ccatattgat aagcgcttcc aagccgatcc aaatgaccaa gccagaagca
     4801 agcaatgttc caagcatatc gggcgcacga cgagcgatca caagtccgcg ccagacgagc
     4861 gcaccaaata aaccgatcaa gcctacgccg ccgaaccacc ccagttcttc cacaacaaca
     4921 gcgtaaatac tatctgtcgg gggcacgggc aagccagtga ctttggaaag cgatttaccg
     4981 atccctacac cgaaccatcc gccgttgacg atggcttcaa acgaacgacg cacatgatag
     5041 gaagcctgca atgggtcttt gattcctgcg acaaaatcag aaacgcgttg ttgacccgta
     5101 aggctgattg aaaccaccaa ccacgccacg gcaaaagcaa tgatcaacaa accgccgatc
     5161 tgtttcaggt caccacctgc gaggaagaag agcaaaccgc cgagcataag cacagttgcc
     5221 gcagcactta agtctggttg caggtagatc aagccgccca caaaaccgag gatgacaccc
     5281 aagggaatca aaccaaatgt aatgtctttc agcatttcgc gcttggcata taaccaaacc
     5341 gcaagataca agatcgatac caactttgcc aactcagaag gttggatcga accattgaag
     5401 atggtacgcg tagcgttgaa acgaacctcg ctcattacca acacgatgat caacatcaaa
     5461 acagtgaacg ccatagcagg cacaatgaat tggcgccaac ggtgataatc gataaacgct
     5521 aaaatcagcg cggctacggt tccaagccca agccacatca actgacgatt gaacatatac
     5581 atcgcgtcgc cgttataggc agcgcgtgaa aagtcccaag aagctgagta caacatcacc
     5641 aaaccaaata cgatcaatgc caccacgatc accaagagtg gcatgtcgaa gccgcccgca
     5701 cgcggacggt cttgaggtga agtttctctt acgaaagttc cgatacccat tttctaaacg
     5761 cttctcctct ctccgcaaaa tccttaaact catcaaagct cgtgccgccc ggtgataaaa
     5821 ggacgacatc ccctgcggat gtaacatccg cagccagtgt aactgcctct ttgagagtca
     5881 ttgcccgatg aacgtccacg gttccgactc cgctgtaggc tgtaattgcg ttctgaatca
     5941 tctcccccgc ctcaccaaag agcaccacat ggtccacacg ttcgtggata agtttggcaa
     6001 gatcgcccca aggaagattt ttgtcacgtc caccaagcat gagcacgatc ggctcactaa
     6061 atgcatggac ggctgctgct gcgcgctcgg gtgctgtagc aatcgagtcg ttataccagc
     6121 gtgcaccatt caattcgcgc accaactcca agcgatgcgg cacaccatgg aattcttcca
     6181 cggcttcgag catatcatcc aacttgaaac cagccgcgtg cccaatggca aaagcagcca
     6241 acacatttgc aatgttatgg tcgccacgta gctgaacttt ttcacgcatc aacaatggga
     6301 cgtaagcaaa cccatctcgt aagctaagca aaccatcgtg aagatatgta ccgttcaagc
     6361 cttcgtctag gtcctgtaag ctgaacgaaa gtaaattccc tttcacacgg ttcttcaaac
     6421 cccaagcgcc tttatcgtcg cgccctaaga tcgcgttatc cttcgcagac tgaaaatcca
     6481 agatatgcgc cttggcattg gtataggctt ccatcgtacc gtggcgatcc aagtgattcg
     6541 gtgtgatgtt caaaatcgcc gcaatgttcg gtgaaatagt catctgctca agttggaagg
     6601 aagaaagttc caatatggct aaatcatcag cttgcatgtc atccacatag ttgatgagtg
     6661 gatcgccaat attgccaccc acaaaagcac ggtcgccttt tgcaagtttt gccatgttgc
     6721 caacgagagt tgttgtggtc gtcttacctg ccgaaccagt gatgccaacc gttttgcatg
     6781 aaaccacttc catgaagatt tgtgagtcat tcgaaagcgg aatatcacgc ttaatagctt
     6841 cttccacaat aggcaaggtc aacggtacgc caccagagat acacaataca tcagttgtat
     6901 ccaataactc aagcggatgc ccacccactg cccaagtcac attcacatca gcgagagatt
     6961 ttttcgcagt gataaggtct tcttcgcgac gcgaatcatt caaggtcacg tttgcgccgt
     7021 gacgtgcaag ccaacgagca agggcgagtc cctgccgggc ggctccaaga atgagaacgt
     7081 gcttaccagt ccaattcgtc atactacact tgcgataatc cgattccaac caaggctgcc
     7141 atcaagccga tgagccaaaa gcgttggaca atttgcgttt cactccaacc cagcaactca
     7201 aaatgatgat ggatgggagt cattttgaaa aagcgctttc cgcctgtcac gcggaagtat
     7261 gtcacctgaa tgacaacgct catcatttca gccaaaggaa taattgcgat caccaacaac
     7321 atgggccact gcccggtcat caacgcgata acagccagta cagaacccaa tgccattgag
     7381 cctgtatcgc ccataatcaa ttgtgcaggg tgaacgttaa accataaaaa gccgaacaag
     7441 gctccaacga ggataaaaca aaaacgtgcc agatagatct gctcttgcat aagagcgatt
     7501 ccaccgaaag cggcaatggc agtcgccgca atcaacccag ctaaaccatc aagcccgtct
     7561 gtaaaattga ctgcatttga ctcagcaaca ataatgaatg cagcgatagg cacatagatc
     7621 caaccgattt gaatttcgaa gaaataaccc ggtaaataca ggtcgggtgc tttaataaaa
     7681 tatttcaaca cataggctgt tgctaaggca agaaccactt gaaaggcaaa tttcgtgcgg
     7741 gcacgcatcc catcaccttt gcgtttgccg tgaatacctt cccaatcatc caccgcacct
     7801 aaaatggcaa agccaaccat cactaccatg ggcaacaaca cagagcgacc cactccgctg
     7861 gcagttacac caatcaaggc aacggcgttt agcaaaccgc taagaagcaa gaccggcaca
     7921 ataaacatca ccccacccat ggttggggtg cccatcttca cccgatgacc ttccggctgt
     7981 tcaatacgga tgatcttccc caacttaaag tgccgcaaaa tacgcagcaa cggatttccc
     8041 caaatggcag tcatgataaa agccaacccc gccaggctta gggaaagagc
//
LOCUS       DAOQNJ010000045         8072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4569_length_8072_cov_9.671698, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000045 DAOQNJ010000000
VERSION     DAOQNJ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4569_length_8072_cov_9.671698"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLG52_02410"
     CDS             <1..723
                     /locus_tag="PLG52_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40322.1"
                     /translation="NFTTRITDAQEKDSSPSWSPNSQQLAFESFRDGNIEIYIANADG
                     SNPIRLTNDPAGDSNPIWSPVSNEILFTSNRFGNSDLMLLNLNGTVSTLTTSRAPDNA
                     AAWSPDGNFIAYQSFSGDISNICIIGRDGLNQVCPSIPAEYGTPVWSPNGTQIAASTL
                     SNGVYSISLFNSRDGSSQQISQPGIEPRGTPAWSPDGLRLVLQTQADGDMELFSVFVQ
                     TNEFTRITTIAGYDGEPTWIVQ"
     gene            complement(775..1533)
                     /locus_tag="PLG52_02415"
     CDS             complement(775..1533)
                     /locus_tag="PLG52_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB40323.1"
                     /translation="MKNKKITLFVVLLIMGILLVACGGGTPPAEETQIVDATQAPVEA
                     TATIQPVSTVDLKLDPANLASDNAKAAVPYLYEGLVRLQDGAVVGALASTFTVSEDGL
                     DYIFDLRTGVTFHDGTLLNADTVVLNFNRWFDPADVNRGSGEYAAWVANFGGFKGELT
                     EDGKPKSPVDGIEKQDEFTVIIHLNTPDPDLLTKLTDPAFSIMSASSFAGNDGGTGPY
                     KAASNDGTTITLEPFAGYWDPAAIPSANVEIPAP"
     gene            1597..>1946
                     /locus_tag="PLG52_02420"
     CDS             1597..>1946
                     /locus_tag="PLG52_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40324.1"
                     /translation="MLPESPLAIIESRLRYLLPSEMYAEMWGNPSMEIMIRVHNHLRT
                     LQRIIHDYTSRQIDVSQLKPGQVKTEWQYGTMMFTDLAGFTKLMEANASKGRDGAENL
                     LKELTKYFSTMISI"
     assembly_gap    1947..2046
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2047..3006
                     /locus_tag="PLG52_02425"
     CDS             <2047..3006
                     /locus_tag="PLG52_02425"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB40325.1"
                     /translation="VLLGKDVQKAKLTESKGRNERVNVSVTAYELAKDSFRFDEGEPD
                     YHLVVDDLTADSLGDYEITPMGRRMASSVLFDLKDKKEVLSNITELLNSIEPLASYIP
                     DPVLSMLVESAADRKIRPDFPTPTIMFVNFIGLPEAADRALPGEERKLALSFSKAFSL
                     INAAVEARGGVLKKVTYHLSGSDIVIYFGVPNAHTNNEMRAASAAVAIREIVESINLK
                     APTIGGIKPEVYCQIGINMGPAFVAEVGDPRGRREFNVLGDTVNTAARLMNRAQKNQI
                     VLSETVKQAIESKYECTPLGEIELKGKSHPMSLYELTKKKPKE"
     gene            complement(3049..3408)
                     /locus_tag="PLG52_02430"
     CDS             complement(3049..3408)
                     /locus_tag="PLG52_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40326.1"
                     /translation="MPGLFDRLDKEIKDNQKKDGISPLDIAKLPPAMRKIMRVMLREL
                     QASYPRMCEVVDAMPEQDRLTRTELDESLATLTEQLWLIRIGEKEKAIYKVNLRRKTG
                     STLMAGIWSSLDTKLKR"
     gene            complement(3420..3632)
                     /locus_tag="PLG52_02435"
     CDS             complement(3420..3632)
                     /locus_tag="PLG52_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40327.1"
                     /translation="MKCWHCEQEARASCSFCGRFVCKDHASTMSTFITMFVGANNTPK
                     GLAVANVIWCGECEPQPEPISMPELY"
     assembly_gap    3807..3906
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3907..4585)
                     /locus_tag="PLG52_02440"
     CDS             complement(<3907..4585)
                     /locus_tag="PLG52_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNB40328.1"
                     /translation="MSMGKIVSIHSFRGGTGKSNTTANLAAQVALAGKRVGVVDTDIQ
                     SPGIHVLFGLDDEKMGKTLNDFLHGRATIREVGFSVGANTGTEDGRAKLAGKDIWLFP
                     SSIKGGEISQIIKEGIDFNVLNEGLQTAISEFDLDYLFVDTHPGLNEETLLSIATSDI
                     LIIILRPDNQDLQGTSVTVDVARSLDVPNLLLMVNKALPKHLNHPEFNKEIEGIFDAP
                     VTGVLPLS"
     gene            complement(4601..5338)
                     /locus_tag="PLG52_02445"
     CDS             complement(4601..5338)
                     /locus_tag="PLG52_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB40329.1"
                     /translation="MSGDILQKLSKTEFFKEFSEEALGAVAGKATLRHFAANEALMRK
                     GDPADSFFVILDGHVKIVTTGARGEEIIINKSNPGETIGELALVDERPRSAGVIALDD
                     VEALELTKDALFSLLDQRLDVSLGILRAFSNRLRFSTTYIEKVIDWSQKTAEGDYSFL
                     DATQPIGNSVRNDDDKAAQLLSTFYSMVHRVKAREEGLKQQVEKLTFQIDQERRKREF
                     EEITGTEFYAKLKEQAQTIRKKRSQDS"
     gene            complement(5335..6438)
                     /locus_tag="PLG52_02450"
     CDS             complement(5335..6438)
                     /locus_tag="PLG52_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNB40330.1"
                     /translation="MLVIALLIAFTFPIAFLILLDRFDLYNTGRFHFNLLTLAGGLVA
                     YFFAAQINPWVVNMGWASWGTVVRFVAPVVEEILKSLILIYLVQRADFNYVVDGALYG
                     FGAGIGFAVIENYEYVTGNAQIALVLALVRVFSTNLMHATGSGLIGTALAYSRGDKNK
                     RQGLLIILGGYAVAMLFHGLFNTMVNAGAFLVLAIAYGVIGLWLIWTIIRRGMTVQKD
                     WVAEKLGMADRVTLEETKVVKNIETVNEVLRPVEQRFGAEKASLVRNLIYKQAEIGIK
                     RKLLESVASEARKNEMEAIIKDLAAEVDVLRQNIGAYCMMMVREVYLGQDLQMWNLLN
                     ARVAAAGLGQKGGGLWDRMATRVKQNPDEAEKP"
     gene            complement(6568..>8072)
                     /locus_tag="PLG52_02455"
     CDS             complement(6568..>8072)
                     /locus_tag="PLG52_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40331.1"
                     /translation="GNAGGSGNGGGGGNGGGGGGGNGGGGGGNAGGSGNGGGGNGGGN
                     AGGGSASDPGTGDPSVQNPDGVVVRGSKQDSSDPCLDAGQLPCGKGDHGKGNPVNGSL
                     HANCRAAQGEVDPVCAPKPPKDIIPDGGGNNGNGGGGGGGGGGTPPVVVDPPILPTPP
                     VVEVTAAPKVKVDDNGVASCPNWIMFHSFRSGNLDIFRLDGAEGVDGVQAINLSQDNS
                     QDSRPSRSPNDEFVVFQSDRDGNVELYLTDSKGSSQSRLTNTKSNNINAMFGPDSKSV
                     IYQSDRNGNWDIFLLNKDTGEELQLTSDAGDDVNPFYSPDNKWITFQSNRTGSWNVFV
                     LDVSTGTEYQVTSFSTDVLFPSWSPNGKQLAFLINMTGTWDLYVSDIQGKGFKQITTG
                     GDAQNATWSPEGDRLAYQVTYNGNTDIFTFDLTTNEEYQLTNFGGVDSAPTWNCGGTD
                     VSFTSNREGDPNIYSTWWQGGGDIYGITVDPSTDKWSQWSPSKEPGSRGY"
BASE COUNT         2126 a         2082 c         1671 g         1993 t
ORIGIN      
        1 aatttcacaa cccgcatcac agacgctcaa gaaaaagact cctccccttc ctggtctccc
       61 aacagtcaac aactagcttt tgaatccttt cgtgatggca acattgaaat ttatattgcc
      121 aatgccgatg gttccaatcc cattcgcttg accaacgacc ccgccgggga cagcaatcca
      181 atctggtcac cggtctcgaa tgaaatcctt tttacaagca accgcttcgg caattcagac
      241 ttgatgttgc tcaacctcaa tggaacggtc tctactctga caaccagccg cgctcctgat
      301 aacgccgccg cttggtcgcc agacggaaac tttatcgcct accaatcttt tagcggcgat
      361 atctcgaata tctgtatcat cgggcgcgat ggacttaacc aagtttgccc atccattcct
      421 gcggaatatg gaacaccagt ctggtctcct aatggtactc aaatcgccgc cagcactctc
      481 tcaaatgggg tttacagcat ttcccttttc aatagccgag atggtagctc acaacaaata
      541 agccagccgg gaattgagcc gcgcggcaca ccagcctggt cacctgatgg gcttcgatta
      601 gtcctacaaa cccaagcaga tggagacatg gaactcttct ctgtatttgt tcaaaccaat
      661 gaatttacaa ggattaccac tatagccggg tatgatggcg aaccaacctg gattgtacaa
      721 taaccaataa taaaagaccc cgaacgcaat gttcggggtc ttttattatc aaaattaagg
      781 agcagggatt tctacattcg cgcttgggat tgcggctgga tcccaataac cagcaaatgg
      841 ttctaatgta atggtagtgc catcattaga tgccgcttta taaggtccag tcccaccatc
      901 atttccggca aaactcgaag cactcatgat cgaaaaagct gggtcggtta acttggtcaa
      961 taagtcaggg tctggtgtat tcaagtgaat aatcactgta aattcgtcct gcttctcgat
     1021 tccgtcaact ggagatttcg gtttcccatc ttctgtcaac tcgcctttga agccaccaaa
     1081 gtttgccacc caagctgcat attcacctga gcctcggtta acatcggcgg ggtcaaacca
     1141 gcgattgaag ttcaacacta cagtatcggc gttaagcaat gtcccatcat ggaaagtaac
     1201 acccgttcgg agatcgaata tatagtccaa tccatcttct gaaacagtaa aggtggatgc
     1261 caatgctcca accacagcac catcttgcaa acgaaccaga ccctcataca ggtagggcac
     1321 agcggctttg gcattatctg aagctaagtt agcaggatca agcttcaggt ccacggtaga
     1381 gacgggttga atagtagcgg ttgcctcgac aggtgcctgt gtcgcatcca caatttgcgt
     1441 ctcttctgct ggaggcgttc cgccgccgca ggctacaagt aaaatcccca ttattaagag
     1501 aactacaaac aaggttattt ttttgttttt catttttctc tctgccttta gatagaattg
     1561 aaagtaaaca tcttcaattc aagtataatg gaaatcatgc ttccagagtc accgcttgcc
     1621 attattgaaa gccgcttgcg ttatctgctc ccctctgaaa tgtacgccga aatgtggggc
     1681 aacccctcga tggagatcat gattcgggtt cacaatcatc tgcgaaccct gcaacgtatc
     1741 atccatgatt acacatcaag gcagatcgac gtctcccagt taaaaccggg acaggtgaaa
     1801 accgaatggc agtacggaac catgatgttc accgacctag caggtttcac caaactgatg
     1861 gaagccaacg catccaaggg acgtgacggt gcggaaaacc ttctcaaaga attaacaaaa
     1921 tatttttcaa ccatgatctc gatcatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnngtgc tgctcggtaa agatgtgcaa aaagccaaat tgaccgaaag caagggacgc
     2101 aacgagcgtg tgaacgtgtc tgtcaccgcc tatgaactgg caaaggacag tttccgcttt
     2161 gatgaaggcg aacccgacta tcacctggtt gtggacgacc tgacagcgga tagccttggc
     2221 gattacgaaa tcacacccat gggacggcgc atggcaagta gtgttctatt tgacttaaaa
     2281 gacaaaaagg aagtcttgag caacatcact gaactgctca actccattga acctctcgcc
     2341 agttatattc ctgaccctgt cctttcgatg cttgtcgaaa gtgcggcaga ccgcaagatc
     2401 cgcccagact tccccacccc tactattatg ttcgtcaact ttatcggttt gccagaagca
     2461 gcagaccgtg ccctgcctgg cgaagagcgc aaattagcac tcagtttctc gaaagctttc
     2521 tcgcttatca atgcagcagt agaagcgcgc ggcggggtgt tgaaaaaagt gacctaccac
     2581 ctttcaggtt cagatatcgt catatacttt ggcgttccaa acgctcacac caacaatgaa
     2641 atgcgtgctg cctctgcagc tgttgcaatc cgcgaaattg tagaatcaat taacctcaaa
     2701 gcgcctacga tcgggggcat caagcctgaa gtgtattgcc agatcggcat caatatggga
     2761 ccggcattcg tggcagaagt tggtgaccca cgcggcagac gtgagttcaa tgtgctgggg
     2821 gatacggtca atacagcggc tcgcttaatg aaccgtgccc aaaagaatca gatcgtcctc
     2881 tcagagacag tcaaacaagc gattgagtca aagtatgaat gcacgccctt gggtgagatc
     2941 gagctaaagg gcaaaagcca ccccatgagt ttgtatgagc taaccaagaa aaaaccaaaa
     3001 gaataaaaaa acgccgcgat cgcggcgttt ttatttaagt agaattggtt atcgcttaag
     3061 ttttgtatcg agcgaagacc atatccctgc cattaaggtg ctgcccgttt tacggcgcaa
     3121 attaacttta tagattgcct tttccttttc accgattcga atcagccata gttgttccgt
     3181 gagtgttgca agagactcat ctaattcggt gcgcgtgagg cggtcttgct caggcatggc
     3241 atcgaccact tcgcacatcc gcgggtagct tgcttgaagc tcgcgcaaca tcacacgcat
     3301 gatcttacgc atggcaggcg gaagcttggc aatatctaat ggagagatgc catctttctt
     3361 ttggttgtct tttatttctt tatcgaggcg atcaaaaaga ccaggcatgg ttccctcgct
     3421 taatacaact caggcatact gatcggttcg ggttgtggct cacattcacc acaccagatc
     3481 acattagcca ctgccaaccc cttgggggta ttattcgcac ccacgaacat cgtaataaac
     3541 gtggacatgg tcgaagcatg gtctttacaa acgaaacgtc cacaaaatga gcaggatgcc
     3601 cgggcttctt gttcacaatg ccagcatttc ataagaggct cctattttgc attcaggatg
     3661 aacggcacaa cgttccgcaa agccttcgac cattcatggt ctggatatcg cagagaaaat
     3721 aggtctttgc tggcattttc tgccatatcg aaagaaaggg gcaaaacgcc cgttacaggc
     3781 gcatcaaaaa tcccttcgat ttctttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnaaga aaggggcaaa acgcccgtta caggcgcatc aaaaatccct tcgatttctt
     3961 tattaaactc agggtgattc aagtgtttcg ggagtgcttt attgaccatc aacaaaagat
     4021 tcggcacatc caaactgcga gcaacatcaa cggtaacgga tgttccttgc aggtcctgat
     4081 tatctggtcg caagatgatg ataagaatat cagatgtagc aatagaaagt aaagtttctt
     4141 cgtttaatcc ggggtgtgta tctacaaaaa ggtaatcaag gtcaaattcg ctgatcgcgg
     4201 tttgcaaacc ttcgttcagt acattgaaat cgataccttc cttgatgatc tggctgattt
     4261 cgccaccctt aatgctggac ggaaacaacc aaatatcttt cccagctaat ttggcacgtc
     4321 catcttctgt gccggtgttt gctcccactg aaaaaccgac ctcccgaatc gtggcacgtc
     4381 catgcaaaaa gtcattcagg gtcttcccca ttttttcatc atcaagccca aaaagcacat
     4441 gaatccctgg agactgaatg tctgtatcca caacgccaac tcgttttccc gccaacgcta
     4501 cttgcgctgc taaattagca gtagtatttg atttacccgt cccaccacga aaggaatgga
     4561 tcgaaacgat cttgcccatg ctcatgaaaa gcctccgttg ttatgaatcc tgcgaacgtt
     4621 tcttacgaat ggtctgcgcc tgttccttta atttagcata gaactcggtg cctgtgatct
     4681 cttcaaattc acgcttacgc cgttcctgat cgatctggaa tgtcaatttt tctacttgtt
     4741 gcttcaaccc ttcttcacgc gccttcactc tatgcaccat ggaataaaaa gttgaaagca
     4801 attgcgcagc cttgtcatca tcattcctga cgctatttcc aatcggctgg gtggcatcta
     4861 aaaaggagta atctccttct gctgtcttct gcgaccaatc gattactttt tcaatatagg
     4921 tcgttgaaaa tcttaatcgg tttgaaaaag ctcgcagaat accaagagac acatccaaac
     4981 gttggtcaag aagcgaaaac aaagcgtctt tggtgagttc caaggcttcc acatcatcca
     5041 acgctatcac acccgcagaa cgggggcgtt catccaccaa tgccaattcg ccaatcgtct
     5101 cgccagggtt actcttgttg ataataattt cttccccacg cgcccccgta gtaacgattt
     5161 tgacatgtcc atccagaatc acaaaaaaag aatcagcggg gtcgcctttg cgcatcaagg
     5221 cttcattggc ggcaaaatgg cgcaaggtcg cctttccagc aacagcccct aaagcttctt
     5281 ctgaaaactc tttgaaaaat tcggtcttac tcagtttttg caggatgtcg ccactcatgg
     5341 cttttctgcc tcatctggat tttgcttcac tcgggtcgcc atccgatccc ataaaccgcc
     5401 tcccttttga cccaagcctg cggctgcaac acgggcattt aacaaattcc acatctgaag
     5461 gtcttgtccc agatacactt cacgcaccat catcatacaa tatgcaccaa tattttgtct
     5521 caacacatca acttcagctg ccaaatcttt aatgatcgct tccatttcgt tcttacgcgc
     5581 ttcactcgca acagactcga gcaatttacg tttgatgccg atctctgcct gtttataaat
     5641 aagattacgt accaaactag ctttctctgc tccaaaacgc tgttccaccg ggcggagcac
     5701 ttcgtttacc gtctcaatat ttttgaccac tttcgtctct tcaagagtga cgcgatctgc
     5761 catacccagt ttttctgcca cccaatcttt ttgaacggtc ataccgcggc gaatgatcgt
     5821 ccagatcagc catagaccaa tgactccata cgcaatcgcc aaaaccaaaa acgctccggc
     5881 attgaccatg gtattgaaca gtccatggaa gagcatcgca accgcatagc cgcccaagat
     5941 aattaataga ccttggcgtt tatttttatc accacgcgaa tacgccaacg cagttccgat
     6001 cagaccgcta cccgtagcat gcatgaggtt cgtggagaag acacgcacca aagcaagcac
     6061 tagagcaatc tgagcgtttc cagtgacata ttcataattc tctatcacag caaagccgat
     6121 gcctgcccca aaaccataga gtgccccatc cactacatag ttaaagtccg ctcgttggac
     6181 gaggtagatc agaattaaag attttaatat ttcttccact acgggagcca caaatcggac
     6241 cactgtcccc caagatgccc atcccatatt cacaacccaa gggttgatct gtgccgcaaa
     6301 gaaatacgcc accaagccac cagccaaggt aagtaaattg aaatggaacc tgccggtatt
     6361 gtataggtca aaccggtcaa gaaggattaa aaacgcaatt gggaaggtaa aagcgatcag
     6421 taaggcgatc acaagcatgg caaaatcata ccttatctag aaacgacccg caaggaaaaa
     6481 tcattaacaa gccgtatcgg ttttagttgt taaacaaact ccgcccttga gaagttcaag
     6541 ggcggggaat ttcacaaaat gttcagtcta atatccacgc gaacccggtt ctttggatgg
     6601 cgaccactga gaccacttat ccgtggaggg gtctactgta atgccgtaga tatcgccacc
     6661 accttgccac catgtagagt agatattcgg gtcaccttca cgattcgaag taaaggacac
     6721 atccgtgccg ccacaattcc aggttggagc ggagtccact ccgccaaagt ttgttaactg
     6781 gtattcctca ttggtagtta gatcaaaggt aaagatatcg gtattgccgt tataggttac
     6841 ctgatatgcc aggcgatcac cttcaggaga ccaggttgcg ttctgagcat cgccaccagt
     6901 cgtgatctgc ttgaagcctt tgccctgaat atctgaaaca tataggtccc atgtcccggt
     6961 catattgatc aagaatgcca attgcttgcc atttggcgac caactcggga agagcacatc
     7021 tgtagaaaat gatgttactt gatattctgt acctgttgaa acatccagaa caaacacgtt
     7081 ccaagaaccg gttcggttag actggaaagt gatccatttg ttatctggtg agtagaaagg
     7141 gttaacatca tcgccagcat ctgaagtcaa ctgcagttct tcacccgtat ctttattaag
     7201 aaggaagata tcccagttac cattgcggtc actctgatag atcacacttt tgctatcggg
     7261 tccgaacatg gcattaatgt tattggattt ggtattggta agccgagact gtgagctgcc
     7321 cttgctatca gtgaggtaca gttccacatt cccatcgcgg tcactttgaa acacaacgaa
     7381 ttcatcattg ggagagcgac tcgggcgtga gtcctggcta ttatcctgac ttagatttat
     7441 cgcttgaaca ccatcaacac cttcggcgcc atccaaacgg aagatatcta agttaccaga
     7501 gcggaatgaa tggaacataa tccaattcgg gcaggatgca actccattat cgtcaacttt
     7561 tacttttggt gcagctgtca cttcaaccac gggtggagtt ggtaatatag gagggtccac
     7621 tacgactggt ggagtgccac cgccaccgcc gccaccaccg ccaccattcc cattgtttcc
     7681 accaccatca gggataatat ctttgggtgg tttcggtgca caaaccgggt ccacttcgcc
     7741 ttgggcggca cggcaattgg catggagact tccatttaca gggttgcctt ttccatgatc
     7801 gcctttaccg cagggtaatt gtccagcatc taaacagggg tcagagctat cttgcttcga
     7861 tccacgaacc acgacgccgt cagggttttg gacagacgga tcccctgttc cagggtcact
     7921 agctgaacca ccgccagcat tacctccacc attgccaccg ccgccgtttc cactaccacc
     7981 ggcattgccg ccccccccac ccccattccc gccgccgccc cctcccccat taccaccccc
     8041 gccgccattt ccgctaccac cggcattgcc ac
//
LOCUS       DAOQNJ010000046         8048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4585_length_8048_cov_8.448893, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000046 DAOQNJ010000000
VERSION     DAOQNJ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4585_length_8048_cov_8.448893"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLG52_02460"
     CDS             complement(<1..587)
                     /locus_tag="PLG52_02460"
                     /inference="COORDINATES: protein motif:HMM:NF016104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HNB40332.1"
                     /translation="MKRLPDWLTIPFLAFLLSRTLIFGAGILGDTMLPTEEGHWTADE
                     NSPFLSMWAKWDSQYYVDIATNGYWFRPGQQSNVAFFPLYPMTMQVVAKFLGGNLILT
                     GFIISNLAFLGGLIFFYLLTELELGSDSAKRAVYYLAFFPTSFFFSSVYTESMFVFLS
                     IATMYFARKHQWMWAAVFGILTAATRNLGILMWAL"
     gene            complement(584..>1786)
                     /locus_tag="PLG52_02465"
     CDS             complement(584..>1786)
                     /locus_tag="PLG52_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40333.1"
                     /translation="MAAVTEVTPTPQAKATDTPAPQATQTTAPTEEPTQVAKATPAQM
                     DAVGGGNSPYHGVFEGKLADAGWSVINGDWKFANGNFEQTKVDGFDFAAVYTKTAFQN
                     FSIQTGFSHVEGSGGGILFNMPNTDRLAGASMVRYSDKRDNAIVWGYFDENGVYKNQG
                     YADVPNAGADRHTLRVVGGEKSFGIYLDDRLIVQAVPYESGYASGYFGLVTSRTHVIY
                     DEVAVDGVGAVFKGTYDSMDGFTDQRIVSGKWTIENNQAVQTVTDVSDYVWNTGVQAS
                     EYTVSAKITLPNTADSGGGFIIHMSERGSKNNSYIVRLKDGGKGIWWGSINDEGKFKG
                     QGSAPIEGTEKAFTLKLVVKLDTLSIYVNDVEIAKNVAISSQEGWVGLLAYGGKVTFE
                     DVKLEVEK"
     assembly_gap    1787..1886
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1887..2538)
                     /locus_tag="PLG52_02470"
     CDS             complement(<1887..2538)
                     /locus_tag="PLG52_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40334.1"
                     /translation="MLKKYFIFLSTHVNAILNRLRTKIPGLPSNRVLLSTAGVVVAAG
                     GIFVALQVLVAPDQPVTTYTLPYTQTFDDVNLKRWFSQSGVWTIRTGTLMQTVGGEEA
                     GQMHIPLKVPEDAPYHASVFITLKKDTKQAGLSFNAQYPDLIAKQHRVYISRINPETL
                     ELVAGYMDETGAFITQAQVPLTLNTTEFRLDVYVYSNSYLVQVNGQRMIENRPLFYQ"
     gene            complement(2661..5675)
                     /locus_tag="PLG52_02475"
     CDS             complement(2661..5675)
                     /locus_tag="PLG52_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40335.1"
                     /translation="MNSKPRQSRNSRTQNSRTNAVVKAVAQPDPVDIPEATSKRRGLS
                     SVQRIALIVGVVYFSLSLFTFRHVIANIPLVLSGEAVINGDELVPFFNPDSQLIDQAA
                     GKFNQLTNGYEFRVRYAILTTWVRYYKVLPFAIPTVIPLMTYLAYLSVAWLLKNSLPH
                     YKPEVIYQLSAAPVLLIFTVMAYAKITHFYTLILGFSMCLISICLMTYGLIFPQKNPY
                     KPILFACIITLFNPAVHYLVLFALYMAFTVLTLIVMDGITVYQNGSWRNVYRPRTWWN
                     GLKVLWKDWRRLFVEVRFWRCIGAFVMLGLGTLLPYALFVKFYALRGVPNLSETVPGD
                     FYFIQDASISLQHVLAFDMAGIMDKEFTGDYLAKTPRYSNIIYMVLMFTPWLYARARK
                     SIFQNENLQALMAVLYVNTVFTMWATLGYSGPSWLPTFHRTMAFISLRANSLQSGIGD
                     LIVKLFATIVQVLRFPHRFELVTFMMACIIISISLVWLHDVFMQTTYPKLVWILTGKH
                     LPAEKKSRRQNQVAPKKEAGRFIPLLMAVLFMFPLFSNQSYRIVFSSGDFNQFLTPYP
                     VGPLKEVKNALLKLPPGKVVVLPPTETAKVVIDINGVEHKFIDKFHIYYLDLPSYYYG
                     LTGDSDNKHEFFLLLRALYYQQPWWINIARDLDLKYIVVNKELVASTVGGQEYLKEVE
                     KILIPELDSRDAYVKKVMENESYVLYEFTDLPTAERVPLYLDVDWNSFIRILSSNLEL
                     TRYYDLRHTMVVGDLEDFDSLTMVTDDEHESALDLYEKKNKKQFFRPSSVILPFDPEQ
                     ISSSYYLSPMFRLFQFFSDSKYNRLDMITPGLWGTLEGGFIGVPREAPFRIDVTLPEE
                     GEYRLLMRGAISAVDMEMESKLFPEPQRISLASDPSNLAFFDKRLVFSSSRTPLDVSQ
                     YTYQELGMLIPTDVVVVNYQYQFFDLGVVQASKGKYPIYFNKLNDAPMLVEGILVIPE
                     EVYQTLTLPSNVTVVQPNDLCCGSVIIQGEEP"
     gene            complement(5647..6780)
                     /locus_tag="PLG52_02480"
     CDS             complement(5647..6780)
                     /locus_tag="PLG52_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNB40336.1"
                     /translation="MKILFLGTHGQFNLGDELLLETFLSQLGSEHRYAVNSYDPAFTQ
                     GMMTPKFNMEAFHTTRELPRFLKYLLTSDLLFFGGGSIIKELYASVGRNPYSTLLMIL
                     ATVTFAKKIARKKVVMSNIGVGPLMTPRGKMLARWILSQVDFLSVRDEKSHRTTLDLG
                     LSPLKVNLVPDAVFANPPEVFLPNPKPFPPSDKTRVALNLNFDIEKPEAWEPFLQSLA
                     GSLKQLHAETPLEIHALPMQSKFKANDDLSVLKEFSKRIPEIPFVLHDPQTAQEAAEI
                     ISGVDLVLAERLHTLVISSILGKPFYGLVYDVKVQELVDYLGMEEYSRNINEHFCAED
                     LTKDIRSVFTDYEKISIHLTERSTNLRMKLGAYFDELKTKAIS"
     gene            complement(6895..8031)
                     /locus_tag="PLG52_02485"
     CDS             complement(6895..8031)
                     /locus_tag="PLG52_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB40337.1"
                     /translation="MKTIREHLGAKLFLANLIVILVGILILVLTIQVTIPAAFNRHMG
                     MMEGAAMMGNGMGMGQGQGYGRTLFESFRASMFDALTYAVVASVVVALLVSWFLSRRI
                     VAPVQNLADASLSLANGNYSERVQVDGSDEIAKLASRFNHMAAQLEQVESMRRQLIGD
                     VTHELRTPLTSIKGYMEGLGDGVLPANAETFNQIHHEADRLSRLVDDLQELSRVEAKS
                     YSLDLRSVSVSSLVQTTLKRLSPQATAKRITLRSNLPADLEPVLADEDRITQVLVNLT
                     ANAIQYTPEDGEVLISAAQHGNEIHVSVKDSGIGIPQEHLANIFTRFYRVDKSRSRHA
                     GGGSGIGLTIAKHIIEAHGGRIWAESAGEGQGSTFTFSLKGVIP"
BASE COUNT         2263 a         1963 c         1775 g         1947 t
ORIGIN      
        1 caaagcgccc acatcaaaat gccaaggttg cgtgtggcag ccgtgagaat gccaaagacc
       61 gccgcccaca tccactgatg tttgcgggca aagtacatag tagcaatgga aagaaacacg
      121 aacatactct cggtgtagac cgaagagaaa aagaacgagg tggggaaaaa tgccaggtag
      181 tacaccgcac gcttggcgga gtctgagccg agttcaagtt ccgtcaacaa gtagaaaaag
      241 atcaatccgc caagaaatgc caggttgctg ataatgaatc cagtcaagat caaattgcca
      301 cctaggaatt tcgccactac ttgcatagtc atcgggtaaa gcggaaagaa tgctacattg
      361 ctttgttgac ccgggcggaa ccagtaaccg ttggtggcga tatctacata atattgacta
      421 tcccacttcg cccacatcga aaggaatggg ctgttctcgt ctgcggtcca gtgaccttct
      481 tcagtcggca acatcgtgtc gccgagaatg ccagcgccaa atatgagcgt gcggctaagc
      541 aagaatgcca agaatggaat ggtgagccaa tcgggcaggc gtttcatttt tccacctcaa
      601 gtttcacatc ttcaaatgtg accttaccac catacgcgag caaacccacc caaccttctt
      661 gcgaagagat ggcaacattc tttgcgatct ctacatcatt tacataaata ctgagggtat
      721 caagtttgac taccagcttg agcgtgaatg ctttttcagt tccctcaata ggcgcagagc
      781 cttgcccctt gaatttgcct tcatcgttaa tgctgcccca ccaaatgcct ttgccaccat
      841 ctttgaggcg cacaatgtat gaattatttt tcgagccacg ctcactcatg tggatgatga
      901 aaccgccgcc cgaatctgct gtgttgggca aagtgatttt agcgctgacc gtatattctg
      961 aagcctgtac gcccgtattc caaacatagt ctgaaacatc ggttacggtt tgaacagctt
     1021 ggttattttc aatcgtccat ttgccgctga caatgcgctg atcggtaaaa ccatccatgc
     1081 tgtcgtacgt gcctttgaac accgcaccga ctccatcgac tgccacttcg tcatagatca
     1141 cgtgtgtgcg tgaagtaacc aagccaaaat agccggaggc ataaccagat tcatacggca
     1201 ccgcttgcac aatcaagcga tcatcaagat aaataccgaa cgatttctcg ccgcccacca
     1261 cgcgtaatgt gtgacggtct gccccagcat ttggcacatc ggcatagcct tgatttttgt
     1321 acacgccgtt ctcgtcgaag tagccccaca caatcgcatt atcgcgttta tcagaataac
     1381 gcaccatcga agcacccgcg aggcgatctg tgttcggcat attgaagaga atgcctccac
     1441 cactaccctc tacatgtgaa aagccagttt gaatgctaaa gttttggaaa gcggtcttgg
     1501 tgtacactgc cgcaaaatca aacccatcta ctttggtttg ctcaaagttc ccattcgcga
     1561 acttccaatc accattgatg actgaccagc ctgcgtccgc tagtttgcct tcgaagacac
     1621 catggtaggg agaatttcca ccgcccaccg catccatctg cgccggagtt gccttggcaa
     1681 cctgagtcgg ttcctccgtt ggagccgttg tctgcgtcgc ctgcggcgca ggagtatcgg
     1741 tcgcttttgc ttgcggcgta ggcgtgacct ctgtcaccgc cgccatnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnntctg gtaaaacagc ggacggttct caatcatgcg
     1921 ctgaccattg acctgcacca ggtacgaatt gctataaaca tacacatcca accgaaattc
     1981 ggtggtgttg agcgtcagcg gaacctgcgc ctgtgtgatg aatgcccctg tttcatccat
     2041 ataccctgca accaattcaa gcgtttcagg gttgatgcgg ctgatataca cccgatgttg
     2101 ttttgcgatt aagtcaggat attgagcatt gaacgaaagc cctgcctgtt ttgtatcctt
     2161 tttcaatgtg atgaatacgc tggcatgata cggcgcatct tccggcacct tgagcggaat
     2221 atgcatctgc cccgcttctt ctccgccaac tgtttgcatc aacgtccctg tgcggatcgt
     2281 ccacacacca gattgagaaa accagcgttt gagatttaca tcatcaaacg tctgcgtgta
     2341 aggcaaggtg taagtcgtca caggttggtc aggcgcaacc agcacctgca atgctacaaa
     2401 aatcccgcca gcagccacta ccactccagc agtggatagc aatacgcgat tcgaaggcaa
     2461 accgggaatc ttcgtccgca gtctgttcaa aatagcattc acatgagtag aaaggaaaat
     2521 aaaatacttc ttcagcataa taattaattc cattctagtg aaaatagaaa aatactacat
     2581 ttcagactcg ttttcaaaaa actaaagatt ttgcaaatca ataaatacag gttttccccc
     2641 attgttattt tgcgttaact ctaaggctct tccccttgaa taatgaccga accacagcac
     2701 aggtcattgg gttgcaccac cgtaacattg gaaggcaatg taagggtctg gtaaacctct
     2761 tcaggaatca ctaaaatgcc ttctactaac ataggcgcat cattgagctt attgaaatag
     2821 atcgggtatt tccctttgga tgcctgtaca acacccaagt caaagaactg atattggtaa
     2881 tttacaacca ccacatcggt tgggataagc attcccaatt cttggtaggt atattggctc
     2941 acatccaacg gagtgcggct tgaagaaaag actaaacgct tgtcgaaaaa tgccaaattt
     3001 gatgggtctg aagcaagcga aatccgctgc ggttccggga aaagcttcga ctccatttcc
     3061 atatccactg ccgagatcgc accacgcatc agcaggcgat attcaccctc ttcaggcaat
     3121 gtcacatcga tgcgaaatgg agcttcacgc ggcactccaa tgaaaccgcc ctccaacgtg
     3181 ccccacaagc ccggcgtgat catatccaag cgattgtatt tactatccga gaagaactga
     3241 aacaaacgaa acatgggcga gagataatat gaacttgaaa tctgctctgg gtcgaatggc
     3301 aaaatcacac tactcggacg gaaaaattgc ttcttatttt tcttctcata caagtccagc
     3361 gctgattcgt gttcatcatc cgtcaccatc gtcagcgagt caaaatcttc caaatccccc
     3421 accaccatgg tatgacgcag gtcataataa cgagtcagtt ccaaattaga agacagaatc
     3481 cgaatgaatg aattccaatc cacatccaaa tacaaaggca cgcgttccgc tgtgggcagg
     3541 tcagtaaatt catacagcac ataactttcg ttttccatca ccttcttaac ataggcatca
     3601 cgtgaatcca attcaggaat caaaatcttt tccacttctt tcaaatattc ctgtccgccc
     3661 acagtgcttg ccaccaactc tttattaacc acaatgtatt tcaaatccaa atcacgggca
     3721 atattgatcc accaaggttg ttgataatac aaagcccgta aaagtaaaaa gaactcatgc
     3781 ttgttatccg agtcgcccgt caaaccgtag taataactgg gcaagtcaag gtaataaata
     3841 tgaaacttat caatgaactt atgctccacc ccgttgatat caattactac cttcgccgtt
     3901 tctgttggcg gtagcaccac tactttccct ggcggcaact tcaacagagc gttctttact
     3961 tccttcaacg gtccaaccgg gtatggcgtc agaaattggt tgaaatctcc agagctaaac
     4021 acaatacgat atgactgatt ggagaacagc gggaacataa acagtaccgc catcaacagc
     4081 gggataaaac gccccgcctc tttcttcggc gcaacctgat tctgccttct cgattttttc
     4141 tcagccggaa gatgttttcc ggttaatatc cacaccagct ttgggtacgt agtttgcata
     4201 aagacatcat gcagccatac caagctgatc gagataatga tgcacgccat cataaacgtg
     4261 accaactcaa aacgatgtgg gaaacgcaaa acctgaacaa tcgttgcaaa taatttgacg
     4321 atcaaatccc cgatgcctga ttgcaggcta tttgccctca aactaataaa cgccattgtg
     4381 cggtgaaaag ttggcaacca ggaaggaccc gaatacccca aagttgccca cattgtaaac
     4441 acggtgttca cgtataaaac cgccatcaag gcttgtaaat tttcattttg aaatatcgat
     4501 tttctcgccc gtgcatacaa ccaaggggta aacatcaaca ccatataaat gatgttcgag
     4561 taccgcggcg tttttgcaag ataatcacca gtgaattcct tatccataat acccgccata
     4621 tcgaatgcaa gcacatgctg aagcgatatg gaagcatctt gaataaagta aaaatctccg
     4681 ggtacagtct cagaaaggtt gggaacgccg cgcaaggcat agaattttac gaataaggca
     4741 taaggtaata acgtccccaa ccccagcatg acaaatgccc caatacagcg ccaaaagcgg
     4801 acctcaacga acagcctgcg ccagtctttc cataagacct tcaatccgtt ccaccatgtg
     4861 cgcggacggt acacattacg ccacgatcca ttttggtata ccgtaatgcc atccataaca
     4921 atgagggtga gcacggtgaa cgccatatac aaggcgaata agactaaata atgcacagcc
     4981 gggttgaata acgtgataat acaagcaaat agaatcggct tatatggatt tttttgcgga
     5041 aatatcaagc cataagtcat caggcaaatc gaaattaaac acatcgaaaa gccaagaatg
     5101 agcgtataaa aatgcgtgat cttggcgtac gccatcactg taaaaatcag caatactggc
     5161 gcagcactta attgatagat cacttctggt ttatagtgcg gaagggaatt ttttaatagc
     5221 caagccacag acaaatacgc caaataggtc atcaaaggga tgaccgtagg aatggcaaaa
     5281 ggcaagacct tgtaataccg cacccaggtc gtgagaatcg cataccgaac ccgaaattca
     5341 tagccattcg tgagttgatt aaacttcccc gctgcctgat caatgagttg actatcaggg
     5401 ttaaaaaatg ggacaagttc atcgccatta atcacggctt ccccgctcaa gacaagcggg
     5461 atattagcaa tgacatggcg aaatgtgaaa agggataatg aaaaataaac aacccccaca
     5521 atcaacgcaa tacgttgaac ggatgataat ccccgtcgct tggaggtcgc ctcaggaata
     5581 tcaacagggt caggctgggc aaccgcttta actacggcat tagtacgcga gttttgtgtt
     5641 cggctattac gagattgcct tggttttgag ttcatcgaag tatgcgccaa gtttcatgcg
     5701 cagatttgtg ctgcgttcgg ttaggtggat gctgattttc tcataatcag tgaagacgga
     5761 acggatatct ttggtgaggt cttctgcaca gaagtgctca ttgatgtttc tcgaatactc
     5821 ctccatcccc aagtaatcaa ccaattcttg cacttttacg tcatacacca gcccgtaaaa
     5881 aggtttgcca agaatagaag aaatgaccag cgtgtgcaac ctttctgcta ggaccaagtc
     5941 tacacccgaa atgatttctg cagcttcttg tgcggtttgc gggtcatgca aaacaaatgg
     6001 gatttcgggg atgcgtttgg aaaattcttt caagaccgag agatcgtcat tagctttgaa
     6061 tttactttgc attggcaagg catggatttc aagcggtgtc tctgcatgca attgtttcag
     6121 ggaaccagca aggctttgca ggaaaggttc ccaagcctca ggcttttcga tatcaaagtt
     6181 gagattaagg gcaacgcgag tcttatcaga tggtgggaaa ggcttggggt tgggaagaaa
     6241 gacttcgggc gggttggcaa agaccgcgtc tggaacgagg ttaactttca acggggaaag
     6301 accaagatcg agcgtggtgc gatgcgattt ttcgtcacgc acagagagaa aatcgacctg
     6361 actgagaatc cagcgagcaa gcatttttcc gcgaggagtc atcaagggac caacaccgat
     6421 attgctcatg accacttttt tgcgcgcaat cttttttgca aaggtcacgg ttgcgagaat
     6481 catcaacagg gtggaatacg gattacgccc tacactggca tagagttctt tgataatact
     6541 gccgccgcca aagaaaagta aatcactggt cagtagatat ttgagaaacc gcggaagttc
     6601 acgcgtggta tgaaacgcct ccatattgaa cttgggggtc atcatccctt gtgtgaaggc
     6661 ggggtcgtat gagttgacag catagcgatg ttcgctcccg agttgagaga gaaaggtttc
     6721 caaaagaagc tcatcgccga ggttgaattg tccgtgggtg ccgaggaaga ggattttcat
     6781 agaaaagttg tgtccgtgaa ttgataataa cgacttcagc tatgagaaat cctgacagaa
     6841 agattgcaaa gaaaaattat ttgctatttt gttaggcgta aatgtttcag ctttttacgg
     6901 tatgactccc ttcaaactaa atgtaaatgt actgccctgc ccctcacccg cagactctgc
     6961 ccaaatgcgc ccgccatgcg cttcgataat atgcttggca atcgtcagcc caatgccaga
     7021 gccaccgcct gcatggcggg aacgggattt atccacacgg tagaagcggg taaagatatt
     7081 tgcaaggtgt tcctgtggaa ttccaatgcc tgaatctttt actgaaacat gaatttcatt
     7141 tccatgttgg gcggctgaga tcaacacctc accatcttcg ggcgtgtatt gaatcgcatt
     7201 cgctgtcagg ttgaccaata cctgcgtgat gcggtcttca tcagcgagga caggctcaag
     7261 gtcagcgggc aggttcgagc ggagggtgat gcgtttcgcc gtcgcttgag gtgaaagccg
     7321 tttgagggtg gtctgcacga gcgaagaaac gctcacagag cgaagatcca aagaatatga
     7381 cttggcttcg acacggctca attcttgtaa gtcgtcgacg agacgcgaga ggcggtccgc
     7441 ttcgtggtgg atttggttga aggtctcggc attggcaggc aagacgccat cgcccaagcc
     7501 ttccatgtac cctttaatag aagtgagcgg ggtacgcaat tcgtgagtga catcgccaat
     7561 aagctgccgg cgcatggact ctacttgttc aagctgcgct gccatgtgat tgaaacgtga
     7621 cgccagcttt gcaatttcgt ctgagccatc cacctgcaca cgttcagaat aatttccatt
     7681 ggcaaggctc aacgacgcat ccgcaaggtt ttgcaccggg gcaacaatac gacgactcaa
     7741 aaaccaacta accaacaatg ccacaacaac agaagccaca acagcatatg tcagtgcatc
     7801 aaacatgctg gcacgaaaac tttcgaacaa ggttctgcca tagccttgtc cctgtcccat
     7861 gcccatacca ttccccatca tggcggcacc ttccatcatc cccatatgac gattgaatgc
     7921 cgccggaatg gtcacttgaa tggtaagaac cagaatcaaa attccaacca agatcacaat
     7981 aaggttggca aggaataatt ttgcgccgag atgttcgcga atggttttca taatttccta
     8041 taatggct
//
LOCUS       DAOQNJ010000047         8027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4603_length_8027_cov_8.634972, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000047 DAOQNJ010000000
VERSION     DAOQNJ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4603_length_8027_cov_8.634972"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PLG52_02490"
     CDS             complement(<1..610)
                     /locus_tag="PLG52_02490"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNB40338.1"
                     /translation="MSFTSHPEVDQVLSTLLENVQGILGHYFVGMYLYGSLATGDFNP
                     KSSDIDFCVVIQDTLPDEFIAHLEMMHKKIWASPFKWAAKLEGAYLPKSLIRRHTPNG
                     PACPVVNEGKFYLGTQGSDWVIQRHVLREKGVVLAGPDPITLIDYVPPEEIRNAVRGV
                     LEEWWFPMLTDSAWLVNHGSEYHAYAVISMCRALHAIEHGTIV"
     gene            complement(613..2238)
                     /locus_tag="PLG52_02495"
     CDS             complement(613..2238)
                     /locus_tag="PLG52_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNB40339.1"
                     /translation="MLSIHNLNKHFGIQPVLKNINFNISAGERIGLIGANGSGKTTLM
                     RILAGFEHPDSGDVASTRPNLRLGYLAQGMDFSPEQTLHSALGLDSDSQTDPALEVES
                     LALALAQNPNDAALQAQYDSALHKLSGESIQPSAVLEPLGLSHFPADTPIAHLSGGQK
                     TRLMLAKVLLDDPQLLLLDEPTNHLDIDMLEWLEDWLNGFQGAALIVSHDRTFLDNTV
                     TKVLELDSSTHTIKTYDGNYSDYLEQKLLEFDKQMQAYQDQQDEIAQLKRAARQIRSL
                     TVMKKGGKADGGDKFAKGFFGNRATKNVAGRAKNLEARIDHLLTDEKVDKPQGSWKLK
                     LDFGAPEHQSKDVLVTDNLSIGYAPETPLLTNINLFIRAGQRVVLTGANGSGKTTFIR
                     TIVGKLPPLTGSFRLGGATKLGYMAQEQELLNPALNSVQTIQSVSAINETKTRNFLHY
                     FLFKGDAALRPAGDLSFGERARLQLALLVAQGCTFLILDEPINHLDIPSRERFEEALE
                     NFNGTILAVVHDRSFIERFATDVWQAKDGKILK"
     gene            2457..3302
                     /locus_tag="PLG52_02500"
     CDS             2457..3302
                     /locus_tag="PLG52_02500"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNB40340.1"
                     /translation="MTKKVLAVLAHPDDESFGLGGTLALYAQRGYETYYVCATRGEVG
                     TVSPEFLKGFKDTAEMRTDELMRAAKELGLKEVIFLDYRDSGMPGMEANQHPNAQINH
                     PVEEVAGKVVKHIRAIKPDVVITFDPIGGYKHPDHIHIHKATVLAFANADDENFHPEA
                     GEGFKPSALYFQVFPRWFLRVMTNLMPLIGKDPTKWGRNGDINLKELAEVDFPVHVRI
                     NTRSVAEIKTRASAQHASQGGTQMRRGLMGFVTRLFGEREDFMQAYPPVEGEFKRKSD
                     LFEGI"
     gene            3411..4160
                     /locus_tag="PLG52_02505"
     CDS             3411..4160
                     /locus_tag="PLG52_02505"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNB40341.1"
                     /translation="MTQSEPIKPRFQFSRISEVLLQPQHTFALLVSENVGWRMPMLVL
                     TVSTTLVVIVSGFLKARAASMGEVTLPPDWQWWTPDMQNNYMQAQQSMQGPVFTYILP
                     LVGALLGLWLGWLILGALLHFGSTMFGGRGSMQSALMITAWASLPFLVRDGLRIIYML
                     IAQHPIQSPGLSGFTTNAVFASQLLSKVDLFYIWSIVLLVIGFGVTDNMPRNKSISNV
                     VIVSILLLLIQSGVGSLLSGVSGLALQRPFF"
     gene            4162..4890
                     /locus_tag="PLG52_02510"
     CDS             4162..4890
                     /locus_tag="PLG52_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40342.1"
                     /translation="MNSSFIRVVGLKRHYEMGGTLVRALDGVDLEIPSHTFTVVMGPS
                     GSGKSSLLYLLGGLDRPTAGEIYVNGERLDQMDENALALFRRKTMGFIFQSFNLVSSM
                     TALENVAFPMQFAGVPVSQRNEKSQSLLSQVGLAERSHHKPSELSGGQQQRVAVARAL
                     VNDPVLIFADEPTGNLDTQSGVTIMQMLSDLHKSGRTVLVVTHDPRMVRFATNKIFLL
                     DGKVVTEAEYQAVALEPLQGNSGG"
     gene            4891..6600
                     /locus_tag="PLG52_02515"
     CDS             4891..6600
                     /locus_tag="PLG52_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNB40343.1"
                     /translation="MNLARKSFLWVGVIMALLVSSIPTSVLAADLLSVTPTSVVNTAD
                     KTITVTGTGFDNTAVVLLDGSALATTFYNDQTLTAVVPAGYVPKTYNLTVTMSGGTVN
                     IPTPVSFTVTAPAPTNTPIPTPTAAPFARPQLRVSFSGTSGKDGIVSNKPFKFTVNFE
                     NAGSLPAYNTQATFTSADLVPLDTGGVAVLGSVSAGSSVSVDQRFVSNGSLSGKSIIT
                     IDVTLSYNDEKGNAYSDKFTLSASVSGGGGGSGVYYTATPTGVRSGQLIITSYGTDVD
                     PLEPGSRFNLVMTVQNMGNEKAPRVTMIVGGGSSGSSDGTPQPGGVSGGNGEFTNFAP
                     VGTSNIQSLGDLPAGGMVQAKQDLIVNVSTEPGAYPIKVTFSYLNAKGEVVNDEQVIT
                     LLVYSLPNLDISFYRPLDPFFVDQPGALPIQVVNLGKRTAVLGTITVTTDNGTVDGGT
                     SLVGSLDKGGYYTLDATLTPLQTGPAKINFVIEYTDDFNQKRTVEKTIDVNVEEGFIE
                     PTPDPNVPLDTGGMTPPTEETFLQKTWRFILGLLGLDSAPSTSSPDMQSPPTDFQSVP
                     SGG"
     gene            6622..7938
                     /locus_tag="PLG52_02520"
     CDS             6622..7938
                     /locus_tag="PLG52_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40344.1"
                     /translation="MKFSDLVRLVINNLSRRKARVALTAIGVVIGTGAVVILVSLAIG
                     LQMNANEQLYGIGDLSQISVVPAYGGGPMPMGGGSAAVAPEDVKLLTNSALQDLRAIP
                     GVTAVIPRDYMFSSSFVSYKKLQGYSNIFGVGTNDLSELGLEAQQGTTALSRATVVIG
                     YMVAQNFMDPNIRPGQVPPPPPDLYDQQIQLTLTKFDDKNNEIRKNYSLRVAGVLSES
                     GSEADYSIYLPIEVVKSMNEWANNSTINYNKTGYNEVLVRVENPDKTLEVRDAITALG
                     FQANTSLDFVQGINNFYSILQIVLGGVGAIALLVAAIGIANTMAMSILERTREIGLMK
                     AVGATNRDVLSLFLGEAAGIGFIGGLGGVLTGWLLGQMLNVVAVVYLAQQAAQQGGVP
                     PSVAIYTPIWLPVFVLAFSTFIGMLSGLYPALRAANMIPVLALKYE"
BASE COUNT         1995 a         1800 c         2009 g         2223 t
ORIGIN      
        1 aaacaatcgt tccatgctca atggcgtgta atgctctgca catggaaatg acggcatatg
       61 catggtattc acttccatgg ttcactaacc atgctgaatc agtgagcatg ggaaaccacc
      121 attcttccaa aacacctctc acagcgttcc gaatctcttc aggcggtacg taatcgatca
      181 gagtgattgg gtctggacct gcaagcacaa cccctttctc gcgaagtaca tgccgttgaa
      241 tgacccagtc actgccttgc gtaccgagat aaaacttgcc ctcgttgaca acagggcagg
      301 caggtccgtt cggggtgtgg cgacggataa gacttttggg gagatacgca ccctccaatt
      361 tagccgccca tttgaatgga cttgcccaaa tctttttgtg catcatttct aaatgagcaa
      421 taaattcatc aggcaaagta tcttggatga cgacacaaaa atcaatatca ctgcttttgg
      481 ggttgaaatc tccagttgcc agtgaaccat agagatacat gccaacgaaa taatgcccta
      541 aaataccctg cacattttcg agcagggtac tgaggacctg gtctacctct ggatgcgatg
      601 taaaagacat cctcatttca aaatcttccc gtcttttgcc tgccatacat ccgtagcaaa
      661 gcgctcgata aaactgcgat catggacgac agcgaggatg gttccgttga aattttccaa
      721 ggcttcttca aaacgttcac gagaaggaat atcaagatgg ttgataggtt cgtccaagat
      781 taggaaggta catccctgtg cgacgagtaa tgccagttgt aaccgggcac gctcaccaaa
      841 agaaaggtca ccggctgggc ggagcgcggc atctcctttg aagagaaagt aatgcaggaa
      901 gttgcgcgtc ttggtctcgt tgatagctga gacactttgt atggtttgca ccgagttcaa
      961 tgccgggttg agcagttcct gttcctgtgc catatagcca agtttggtcg cgccacccag
     1021 ccgaaacgaa cctgtcaacg gagggagttt gccaacgatc gtacgaatga aggttgtctt
     1081 ccctgagccg tttgcacccg tcaacaccac acgctgacca gcgcggataa ataagttgat
     1141 gtttgtcagc aacggggttt caggtgcgta gccaatggag aggttgtcag tcaccaacac
     1201 atcttttgac tgatgctccg gtgcgccaaa atccaatttc aatttccatg agccttgcgg
     1261 cttgtcaact ttttcatcgg ttaacaaatg gtcgatgcgg gcttcgaggt tcttggctcg
     1321 cccagctaca ttttttgtgg cacggttgcc gaaaaagcct tttgcaaact tatcgccgcc
     1381 atcggctttt ccgcctttct tcatcaccgt caaactgcgg atctgtcttg cagcgcgttt
     1441 cagttgcgcg atctcgtcct gctggtcttg ataggcttgc atctgcttgt cgaactcaag
     1501 cagtttttgt tcaagataat cactgtaatt gccgtcatat gttttgatgg tatgagtcga
     1561 agaatccaat tcgagcacct tggttacagt gttatctaaa aatgtgcggt catgcgagac
     1621 gatcaacgcc gcaccttgga atccgttcag ccaatcttca agccattcga gcatgtcgat
     1681 atcgaggtga ttggtgggtt cgtccagcag cagaagttgc ggatcatcca acaaaacttt
     1741 ggcgagcatg agacgggtct tttgcccgcc agagagatgg gcgatgggcg tatcggcagg
     1801 gaaatgggac aaacccaacg gctccaacac cgccgatggc tggatgctct ctcctgaaag
     1861 tttgtgcaac gctgaatcat attgtgcttg aagcgcggca tcattcgggt tctgagctaa
     1921 tgccaaagca agcgactcca cttccagcgc ggggtcggtt tgggaatctg aatccagccc
     1981 aagcgcggag tgaagcgttt gttcgggtga gaagtccatg ccttgggcga gatagccgag
     2041 gcgtagattc gggcgcgtcg aagcgacatc cccagaatct ggatgttcga acccggcaag
     2101 gatgcgcatg agcgtggtct tgccgctgcc atttgcgccg atgagcccaa tgcgttcgcc
     2161 tgcgctgata ttgaagttga tgtttttgag aacgggctgg ataccgaaat gtttgttgag
     2221 gttatggata ctaagcatat gaacgaactc ctgtagttaa aaagaaaacc gagggcaagc
     2281 ctcggcaata ttaaacgcca atagggaaat gccctgaaaa aatggtttaa ctacttgctg
     2341 tccgtcattc gctgggtatc ctttcttgag aaaaatttta tcacattccg taaagcaggg
     2401 gtaaacttgt ggatagtttg aacgtcaaaa acgcatcatt gatcaaagag gttaacatga
     2461 cgaagaaggt tttagcagta ctggcgcacc ccgacgacga gtcgtttggc ttgggcggga
     2521 cgttggcgct ttatgcacaa cgcgggtatg aaacatatta tgtttgtgcc acacgcggcg
     2581 aagttggcac tgtttctcca gagttcttaa agggatttaa agataccgcc gaaatgcgca
     2641 ccgatgaatt gatgcgtgcc gccaaagaac ttggtttgaa agaagtcatc tttcttgatt
     2701 atcgtgattc gggtatgccc ggcatggaag ctaaccaaca cccgaatgcg cagatcaatc
     2761 atccggtaga ggaagtggca ggcaaggtgg tgaagcatat ccgcgccatt aagccagatg
     2821 ttgtgattac gttcgacccg attggcgggt ataagcaccc ggatcatatt catattcaca
     2881 aagctacggt tctcgctttt gccaatgccg atgatgaaaa tttccaccct gaagctggcg
     2941 aaggtttcaa gccaagtgct ttgtatttcc aggtctttcc acgctggttc ttacgtgtga
     3001 tgaccaacct tatgcccctg atcggtaagg accccaccaa gtggggtcgc aatggcgaca
     3061 tcaatttgaa agaacttgct gaagtagatt tccctgtgca tgtgcgcatc aatacccgtt
     3121 ctgtggctga gatcaagact cgcgccagcg ctcaacatgc atctcaaggc ggcactcaaa
     3181 tgcgccgcgg tttgatgggt tttgtgacac gtttgttcgg cgagcgagaa gattttatgc
     3241 aagcatatcc gccggtggaa ggcgaattca aacgaaaaag tgatttattc gaaggaatat
     3301 agaaaataaa tgaagcggct ggaacaaatc cagccgcttt ttcgttaatc gctattaagc
     3361 atgaaaattt ggtgcatgtt agaattttta cgaggaaaaa cggaatatat atgacccaat
     3421 ctgaacccat taagccacgt tttcagtttt ctcgtatttc tgaagttttg ttacagcctc
     3481 aacacacctt tgccttgtta gtcagcgaaa atgtgggttg gagaatgcct atgctggtgc
     3541 tgacggtttc aaccacactg gttgtcattg ttagcggatt tctgaaagcc cgcgctgctt
     3601 ccatgggaga agtgacgctt ccgccggatt ggcaatggtg gacgcccgat atgcagaata
     3661 attacatgca ggcacaacaa tccatgcagg gaccggtctt tacctacatt cttccgctcg
     3721 ttggggcgtt gcttggtctg tggcttggtt ggctgatttt aggtgccttg ctccattttg
     3781 gctcgacaat gtttggcggg cgaggctcga tgcaaagcgc cttgatgatc acagcatggg
     3841 cgagcctgcc atttctggta cgagatggtt tgcgcattat ctatatgctc attgcgcaac
     3901 atcctattca aagccctgga ctttctgggt ttacgaccaa tgcagtattt gcttctcaac
     3961 tactttcaaa agtcgacttg ttttatattt ggagcatcgt tcttttggtc atcggctttg
     4021 gcgttactga taacatgccg cgcaataaat caatatcgaa tgttgtaatt gtttcaatct
     4081 tgcttcttct cattcaatct ggcgttggtt ctttattgtc aggggtgagc ggtttggcgc
     4141 ttcaacgtcc tttcttttaa tatgaattct tcttttattc gagttgttgg tttaaaacga
     4201 cactatgaaa tgggtggaac ccttgtccgc gccttggatg gcgtggattt agagattcca
     4261 tctcacacct ttacagtggt tatgggacct tccggctcag gcaaaagtag tttgttatat
     4321 ctgcttggtg ggttagaccg cccaactgcg ggcgaaattt atgtcaacgg tgaacgtttg
     4381 gatcaaatgg acgagaacgc gcttgcactt tttcgccgaa aaacgatggg ttttattttt
     4441 caatcgttca accttgtttc aagcatgacg gcattagaga acgtggcttt cccaatgcaa
     4501 tttgcaggcg tgcctgtttc acagcgaaat gaaaaatccc aatccctctt atctcaagtt
     4561 gggctggcag agcggagtca ccataaaccc tctgagcttt caggcgggca acaacagcgt
     4621 gtggctgttg cacgggcttt ggtaaatgac cctgttttga tctttgcaga tgagcctact
     4681 ggcaacctag atactcaaag cggtgtgacc attatgcaaa tgctctcaga cctgcataaa
     4741 tctggtcgga ctgtgctggt ggtaacgcac gatccacgca tggtgcgctt tgcgaccaat
     4801 aagatatttt tgttagatgg aaaagtggtt acagaggctg agtatcaggc tgttgcacta
     4861 gaaccgcttc aggggaattc tggaggataa atgaatttag caagaaaatc ctttttgtgg
     4921 gtcggcgtga ttatggctct tttggtttca agtattccaa cctctgtttt ggctgcggat
     4981 ttgttgtctg taacgccaac atctgttgtg aatactgccg ataagacaat tacagtaaca
     5041 ggaactggat ttgacaacac ggctgttgta ttgcttgacg gctcagcttt agcgactact
     5101 ttttataatg accagacttt gacggcggtt gtccctgctg ggtatgtgcc gaaaacatat
     5161 aaccttacgg tgacgatgtc tggtggaact gttaatatcc ctacacccgt ttcttttaca
     5221 gttacagcac cggcaccaac aaatacacca attccaacac caaccgctgc accatttgca
     5281 cgccctcagt tacgtgtatc tttttctggt acaagcggta aagatggcat tgtttccaac
     5341 aaaccattta agttcactgt aaattttgaa aatgcaggct ctttgccggc atacaatacc
     5401 caggcaacct ttacatctgc agaccttgtc cctttggata cgggcggtgt agcggttctt
     5461 ggaagtgtct ctgcgggttc tagcgtatcg gtcgaccagc gctttgtttc caatgggtct
     5521 ttatctggaa agtctattat tacgattgat gtcacattga gctacaatga cgagaaaggc
     5581 aatgcctatt ctgacaagtt cacactttct gcttctgtat ccggcggggg cggcggatca
     5641 ggcgtctatt acacagccac tccaacagga gtccgaagcg ggcagttaat cattacttca
     5701 tatggcacag atgtggatcc gcttgagccg gggtctcgat ttaatctggt gatgaccgtt
     5761 caaaatatgg gtaacgaaaa agcgccgcgc gttacgatga tcgttggtgg aggttctagc
     5821 ggttctagcg atgggactcc gcaaccaggc ggtgtttctg gtggaaatgg ggagttcacg
     5881 aattttgcgc cggtagggac atcgaatatc caatcgttag gagatttacc tgccggaggc
     5941 atggttcaag ccaagcaaga cctgatcgtc aatgtttcaa ctgaaccggg tgcatatcct
     6001 atcaaagtca cgttctctta tctaaatgcc aagggtgaag tggttaatga tgagcaggtg
     6061 attactttgt tggtttacag cttgcccaat cttgatatta gtttctatcg cccgcttgat
     6121 ccattttttg ttgaccaacc cggcgcgttg ccaattcaag ttgttaactt ggggaaacgc
     6181 accgctgtgc ttggcaccat cacggtcacc acagataatg gcacagtgga tggcggtaca
     6241 tctttggtcg gttcattgga taagggcgga tattacacct tggacgcgac cttaactccg
     6301 ctgcaaaccg gacccgcaaa aataaatttt gttattgaat atacagacga ctttaatcaa
     6361 aaacgtaccg ttgagaaaac aatcgatgtt aatgttgaag aaggctttat tgagccaaca
     6421 cctgacccga acgttcctct ggatacgggt ggaatgactc cgccaactga agagacattt
     6481 ctacaaaaaa catggcgctt cattcttgga cttttaggtt tagacagcgc tccgtcaact
     6541 tcttctcccg atatgcagtc tccacctaca gactttcaat ccgtgccgag tggcggataa
     6601 gatgatgaat ctggttaaca catgaaattc tcagatctag tccgtttggt catcaataac
     6661 ttgagccgtc gtaaagcgcg cgtggccctt actgcgatcg gtgttgtcat tggcacaggt
     6721 gcggttgtga tccttgtgtc tctagctatt ggcttgcaga tgaatgctaa tgaacagtta
     6781 tatgggattg gtgaccttag ccagattagt gtcgttccgg cttatggggg aggacccatg
     6841 ccaatggggg gcggaagtgc cgcggttgca cctgaggatg tcaaacttct tacgaatagc
     6901 gctttgcaag atttgcgtgc tatccccggc gtgacagctg tgattccgcg cgattatatg
     6961 ttctcaagtt cgtttgtatc ctataagaag cttcagggct atagcaatat atttggagta
     7021 ggtacgaacg acctttcgga gcttgggtta gaggcacagc aagggactac ggcattgagt
     7081 cgtgctaccg tggtcattgg gtatatggtg gcgcaaaact ttatggaccc aaacattcga
     7141 ccagggcaag tcccaccgcc gccgccggac ctatatgacc agcaaatcca attaaccttg
     7201 acgaagttcg atgataagaa taatgaaatc cgcaaaaatt acagtttacg ggttgctggt
     7261 gttctttccg agtctggttc tgaagcggat tattccatct atctcccaat tgaagtggtt
     7321 aagtccatga atgaatgggc gaataattcg accataaatt acaacaagac aggatacaac
     7381 gaggttttgg tacgagtaga aaaccctgat aaaacccttg aagtacgtga tgccatcacc
     7441 gccttagggt tccaggcaaa tacttcatta gattttgtgc aaggcatcaa taacttctat
     7501 tcgatcttgc agattgtgct tggtggggtg ggtgccattg ctttgcttgt agctgccatt
     7561 gggattgcaa atacgatggc aatgtctatt ctggagcgca ctcgagaaat cggattaatg
     7621 aaagcggttg gcgctactaa ccgtgatgtt cttagtctct ttctaggcga agcggcgggg
     7681 attggtttta ttggcggttt aggcggtgtg ttgacaggat ggttattggg gcaaatgtta
     7741 aacgtagtcg ccgtggtata tcttgcccaa caggctgccc agcaaggtgg cgttccgccc
     7801 agtgtggcga tttatacgcc tatttggctt ccggtttttg ttttagcgtt ttctactttt
     7861 attggcatgt tatctgggct ttaccctgcc ttacgagcag ctaacatgat tccagtttta
     7921 gcattgaaat acgaatgaaa aacactttct tcaattgtat aattagaaga agatattaag
     7981 tggaaagtag aggtaaatca tatgccagag aaacgaactg ttccacg
//
LOCUS       DAOQNJ010000048         8018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4611_length_8018_cov_9.019339, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000048 DAOQNJ010000000
VERSION     DAOQNJ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4611_length_8018_cov_9.019339"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1548)
                     /locus_tag="PLG52_02525"
     CDS             complement(142..1548)
                     /locus_tag="PLG52_02525"
                     /inference="COORDINATES: protein motif:HMM:NF009881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB40345.1"
                     /translation="MPDLFDHAMQERMKSEAPLAARMRPRTLEEYIGQEHIIGEGKLL
                     RRAIEADRLFSSIILWGPPGTGKTTLAQVIANTTRSHFVTISAILAGKADLRVVIDEA
                     LERRRLHNERTILFVDEVHRWNKAQQDALLPHVENGTFTLIGATTENPYFEVIKALIS
                     RSRVFQLRNLNQAETGTLLDRALVDKERGYGNKNIQFDEDARNHLIDVASGDARNALN
                     ALELAVESTSPDQDNIIHITLDVAQESIQRRAVLYDKDGDAHYDTISAFIKSVRGSDP
                     DAALYWLAKMIYAGEDPKFIIRRLIILAGEDIGLADPMGLVVASSAAQAFDYIGLPEG
                     IYPIVEATLYLSTAPKSNSAGAYFNAMKKIEEEGQVSVPRHLMDGNRDAAAMGHGKDY
                     VYPHDMDGHFTPQQYLPKRLLGTYFYSPSEEGYETQVKSRLEMWREAQRKALGITEVK
                     TMPAMNEEQIQEMKRKIK"
     gene            complement(1578..1979)
                     /locus_tag="PLG52_02530"
     CDS             complement(1578..1979)
                     /locus_tag="PLG52_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB40346.1"
                     /translation="MLNFKAETQNVRVPVSVLRITGDIDSSNFQTFQTQVDDLVKNGA
                     RHILLDFSNVKRISSAGLRVIHNLFNKLRDIHKDVNDDELRKQMSSGAYKSPYLKLFN
                     LAPQIAEIFSLSGFDIYIQTFDDETEAVQSF"
     gene            complement(2026..2766)
                     /locus_tag="PLG52_02535"
     CDS             complement(2026..2766)
                     /locus_tag="PLG52_02535"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNB40347.1"
                     /translation="MPTPIRYLIFDLGGTLMHSRADWGPIHEQADEALSQSLRENGIE
                     LSAEVFRSRLHDYYQQRDKDFYETTYHLVLRELLKELGHAEVAESVLRSALDALYVIT
                     QSNWELESDAMETLENLKSQNYKLGIFSNAGDDKDVQELVESFGIRSYFDFVLTSAAC
                     FYRKPHPRAFEIALAQWNATPAEAAMIGDSLEADIIGANELNITSIWLTRRAQFKTED
                     MHRIKPNFSLRKLSELPPTLDLLSFSRR"
     gene            complement(2821..4512)
                     /locus_tag="PLG52_02540"
     CDS             complement(2821..4512)
                     /locus_tag="PLG52_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB40348.1"
                     /translation="MKTENTSEQRFENMVTIMIASVAIWVAITAYFQNYASNISDQAR
                     RRAQQYAIEATKTEVNGTIQFSYDWQGAFQTWRELSWQITAAQQNGDTKAVERYQELQ
                     ARIAPLSKMLAPQYFDPTVGWPESYKYEAESYLVESTRLSETYLAESDLGNFTDNVAD
                     SLIVQITLLTVSLSLYGLSMALKGRVRWLFIIVGSGIVGVCVLWLGWSLLELLIRPEV
                     NQAAITAYSEGVGLAYQGRYQESIDKFTQAINENQYYAKAYYERGLSYYELGDLQTAI
                     SEMEEARTQGMQEDVSLNWNLGWTYYLAGQYDNAIEINEPILSDNPEVLGMRMNQAIS
                     YLSKGDFENSQKQYDLLLQEAQRQVNEARSQGAEPSASLWFYMDAGALDLQNLIDQLD
                     NNPKDWTQAPEANLITGDHSAIRDFATSQMKRLKESTVALEYTGQLPNTQSEIQVEPF
                     VFGQITGTDAQGFITGFEPASNNIIPYGEDSFTLEFTYSGTPPKQMLWKVYVNGYEDQ
                     SLRIVSTDDISDGATWYKTFGYSYTNIFILSPGEYTVELYADNILVQRGTFYVQE"
     gene            complement(4509..5144)
                     /locus_tag="PLG52_02545"
     CDS             complement(4509..5144)
                     /locus_tag="PLG52_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40349.1"
                     /translation="MKQDSFKSLIAVLTAIVTVLGATAACLASVAVSTAGDNDFAGLD
                     ASIRGQKAEIINYVVAYEHYRAFTSYVRYNELGNLMYDPGADQDTDARNGAFQREVWG
                     IASGISSVFFSPRYITSEGQYDLERELQEEYAKDAQSEDLNPAPYFEESDRQRNISAT
                     LTADMIVFAVSFWFLTLAQATEKKIKYVWAVLGGLMGLAGIIGIFIGRFLQ"
     gene            complement(5175..5951)
                     /locus_tag="PLG52_02550"
     CDS             complement(5175..5951)
                     /locus_tag="PLG52_02550"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNB40350.1"
                     /translation="MPELKYSSAELMIINAARLLRDGDVVFVGVGQPNLACNLAKRTH
                     APNLVMIYEAGVIGAEPERLPLSIGDPTLVSGALSVVSMYDIFANYLQRGNVDVGFMG
                     GAQIDKYGNINATTIGDYAHPKVRLPGSGGSQEIAAWANRCYIMTPHQKRRFPEKVEF
                     MTSAGFIDGKGAREARGMRGGGMLAVVTDIGILEPDESGEMMLTALHTGRTAEEAKAN
                     TGWNLKVASTLKTTEQVTKKELRILREELDPTGIYLKGAA"
     assembly_gap    6064..6163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6164..6992)
                     /locus_tag="PLG52_02555"
     CDS             complement(<6164..6992)
                     /locus_tag="PLG52_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB40351.1"
                     /translation="MNPIPHFDFGGNGEPLHFLHANGYPPECYTSLLEHLKHNYHLFG
                     MKLRPLWDGANMDDLHDWHPYSEDLLRLLSDRETGPVIGVGHSIGGIVTLRAAMHEPK
                     RFRAVILLDPVLFVPSTMNIWNFVRAIGIGEKAHPLIQTAKRRRREFTDLESVFRSYR
                     TKPVFRYMSDESLKQYIVGITKPKASDGYELVYTPEWETHIYMTGMRDYDLWRGLANF
                     DVPTLIIRGDETDTFLPNAEKLVKKKNPKIQIHTMKKATHILPLEHPKEVADIIKNFL
                     "
     gene            complement(6994..7896)
                     /locus_tag="PLG52_02560"
     CDS             complement(6994..7896)
                     /locus_tag="PLG52_02560"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNB40352.1"
                     /translation="MSKLIPLKKAIAEYVSDGDVVYAAGFTHLIPFAAGHEIIRQGKK
                     NLTLARATPDLIYDQLVAAGCAKKVIFSYMGNPGVGSLRIVRGAIERGELEWEEYSHF
                     GMITRLQAGASGLPFLPMNQTGAEDLEKVNPQIKRIPDPYGGKDVIVVPALNPDVAIV
                     HVQRADKNGNAHLWGIIGEQKEAAFAAKKVILTAEEIVDESVIRSDPNRTMIPGIVVS
                     AVCHVPFASHPSYSQGYYDRDNEFYLAWDKISESKELTQKYLDEWVYGVKDRNAYWKK
                     LGEKTHKRLKVKAQYSEKINYGKY"
BASE COUNT         2028 a         1853 c         1829 g         2208 t
ORIGIN      
        1 gcacctcacg cagcgcctcc cccaaagcct gagcttgttt ctgccccttt tcatttaggt
       61 gcacgccggg aattcgccct gccattttcc ctgttttgac atactcattc tcaccgtgac
      121 ggattagtaa taataaggtc attatttgat cttcctcttc atctcttgga tctgttcttc
      181 gttcatggct ggcatagtct tgacttctgt aattccaagt gctttgcgtt gggcttcgcg
      241 ccacatttcc aaccgtgact taacctgcgt ctcatagcct tcttccgatg gagaatagaa
      301 gtaggttccg agtaggcgtt tgggaagata ttgttgcggc gtgaaatgac cgtccatatc
      361 atgcgggtag acgtaatctt taccgtgacc catagctgca gcatcacgat ttccatccat
      421 gaggtggcgt ggaaccgaaa cctgcccttc ctcttcgatc tttttcatgg cattgaaata
      481 ggcaccagca ctatttgatt taggggcagt ggaaagatag agcgtcgctt cgacaatagg
      541 gtaaatccct tcgggaagcc caatatagtc aaatgcttgt gcggcagaag aggctacgac
      601 caagcccatc gggtcagcca agccaatgtc ttcgccagca agaatgatga gacggcgaat
      661 gataaatttg gggtcttctc cagcgtagat catttttgcg agccagtata aggctgcgtc
      721 ggggtctgag ccgcggaccg atttgataaa agccgaaatc gtatcgtaat gtgcgtcgcc
      781 gtctttatcg tagagcactg cccgccgttg aatggactct tgcgcgacat caagggtgat
      841 atggattata ttgtcctggt cagggcttgt ggattcgact gccagttcca atgcgttgag
      901 cgcatttcgg gcatccccag aagcgacgtc aataagatga tttcgagcat cttcatcaaa
      961 ctggatgttt ttgttcccgt agccacgttc tttgtctacc agcgcgcggt ctaacagagt
     1021 gccggtttca gcttggttta ggttgcgtag ttgaaagaca cgcgaacgcg aaattaacgc
     1081 tttaataact tcaaagtagg ggttttcagt cgttgcacca ataagtgtga aagtgccatt
     1141 ttccacatga gggagaaggg catcttgttg ggctttattc cagcgatgaa cttcatcaac
     1201 gaaaagaatg gtgcgttcat tgtgcaaacg gcgacgttcc agtgcttcat cgattaccac
     1261 acgcaagtct gctttgcctg ccaaaatagc agagatggtc acaaagtgac tgcgtgtggt
     1321 atttgcaatc acttgtgcaa gtgtagtttt gcctgtgccg ggaggtcccc ataagatgat
     1381 agaggaaaac aaacgatccg cttcaatggc gcgacgtaaa agtttccctt caccaataat
     1441 atgctcctga ccaatatatt cttctaatgt gcgtggacgc atgcgcgcgg caagtggcgc
     1501 ttcagatttc atccgttctt gcatggcgtg gtcaaagaga tctggcatgg agaggattat
     1561 agcgtgaagt aaattgtcta gaaagattga acggcttctg tctcatcgtc gaatgtttga
     1621 atataaatat caaacccact aagtgagaaa atttctgcaa tttgcggtgc caagttgaaa
     1681 agtttcaaat aaggtgattt atatgcccca gaactcattt gttttcgcaa ttcatcatcg
     1741 tttacatctt tatgaatatc acgcaattta ttgaacagat tgtgaataac gcgcagccca
     1801 gcactgctaa tgcgcttgac gttgctaaag tctaggagaa tgtgccgagc gccattctta
     1861 accagatcat ctacttgtgt ttgaaaagtt tggaaattcg acgagtcaat atcccctgtg
     1921 attctcagga cactcactgg tacgcgcaca ttttgtgttt cagctttgaa attcaacatt
     1981 aacatttcct ttgatgagtt ttgattttag atgaagtgct ttggtctaac ggcgggagaa
     2041 agagagtagg tcaagcgtgg gtggcagctc tgagagtttg cgcaggctaa agttaggttt
     2101 gatgcgatgc atatcctcag ttttgaattg tgcacggcgt gtcaaccaaa tggaagttat
     2161 gttcaattca tttgcgccaa taatatccgc ttcgagacta tcaccgatca ttgccgcttc
     2221 ggctggggtg gcgttccatt gcgcaagagc gatctcaaac gcacgcggat gtggtttgcg
     2281 ataaaaacat gccgcagagg taagcacaaa atcaaaataa ctgcgaatac caaaggactc
     2341 tacaagttct tgaacatctt tgtcatcgcc agcattggaa aagatgccta gcttataatt
     2401 ttgcgacttg aggttttcga gtgtctccat ggcatcagac tctaattccc aattgctttg
     2461 ggtgataacg tacaacgcat ccagcgcgga ccgtaaaacg gattctgcga cctcagcatg
     2521 acccaactct ttgagcaatt ctcgcaagac aagatgatag gttgtttcgt aaaaatcttt
     2581 atcgcgttgt tggtaataat cgtgtaatct ggagcggaaa acctcggcgc tgagttctat
     2641 accgttttcg cgcaaagatt gtgacagcgc ttcatctgcc tgttcgtgaa taggacccca
     2701 atctgcacgc gagtgcatta gggttccgcc gaggtcaaag atcaaatatc gaataggtgt
     2761 aggcatgttt tttagtgggg agccgttcga gaaaacgact ccctgtctta taaatgataa
     2821 ctattcttgc acgtagaagg ttcctcgttg tacaagaata ttatctgcat aaagttccac
     2881 ggtgtattca cccggactca aaatgaaaat atttgtataa ctatacccga aagttttgta
     2941 ccaagtggct ccatcgctaa tatcatcggt agagacgatc ctcaaggatt ggtcttcata
     3001 cccgttgaca tataccttcc acagcatttg tttaggagga gttcctgaat aggtgaactc
     3061 gagtgtgaaa gagtcttcgc catacggaat gatgttatta cttgccggct cgaagccagt
     3121 gataaaacct tgcgcatcgg ttccggtgat ttgcccaaag acaaacggct caacttgaat
     3181 ttcactttga gtattcggca gttgacccgt atattccaat gctacagtag attctttcaa
     3241 acgtttcatt tgtgaagtag caaagtcacg aatggcagaa tgatcgcccg tgatcaaatt
     3301 tgcttctggg gcttgggtcc aatctttggg attgttatct aactggtcaa ttaggttttg
     3361 aagatccaaa gcgccggcat ccatgtaaaa ccataaggaa gcggaaggtt ctgcaccttg
     3421 tgagcgtgcc tcattcactt gcctttgtgc ttcttggagc agaaggtcat attgtttttg
     3481 cgaattctcg aaatcccctt tggaaagata actgatggct tgattcatcc gcattccaag
     3541 cacttcaggg ttatcactta agatgggctc gttaatttca atcgcattat cgtactgccc
     3601 ggcgaggtaa tacgtccaac ctagattcca attcaggctc acatcttctt gcataccttg
     3661 agtacgagcc tcttccattt cagaaatggc agtttgcagg tctcctaatt cataataaga
     3721 aagtccgcgt tcgtaatagg cttttgcata atactgattt tcgttgatag cttgcgtgaa
     3781 tttatcaatt gattcttggt atcgtccttg atatgcgagc ccaacacctt cagagtatgc
     3841 tgtaatagcc gcttggttga cttcagggcg aattaacaac tcaagtaaag accatcccag
     3901 ccagaggaca caaaccccaa caatgcctga gcctacgata ataaacaacc agcggacgcg
     3961 tccctttaat gccatggaaa gaccataaag cgatagagaa accgtaagca aagtaatttg
     4021 cacgatcaat gaatcggcaa cgttgtctgt aaagttccct aaatcagact ccgccaaata
     4081 cgtttctgag aggcgtgtcg actcgaccaa ataggattca gcttcatatt tgtatgattc
     4141 gggccaccca acagtcgggt caaagtattg aggcgcaagc attttgctaa gcggggcaat
     4201 acgggcttgc aactcttgat agcgttccac cgcctttgta tctccatttt gttgcgcagc
     4261 tgtgatctgc cagcttagtt cgcgccaggt ttgaaaagcg ccctgccaat catagctaaa
     4321 ttgaatggtc ccgtttacct cggtcttcgt agcttcaatg gcatattgtt gggcacggcg
     4381 gcgtgcttga tctgaaatat tggaggcgta attttggaaa taagcagtga tcgccaccca
     4441 gatcgcaacc gatgcgatca taatggtgac catattttca aagcgttgtt cagaggtatt
     4501 ttcggttttc attgtaagaa cctcccaata aaaattccaa taatacctgc caagcccatc
     4561 agaccaccca gcacagccca aacatactta attttctttt cagtggcttg ggcaagagtt
     4621 agaaaccaaa atgaaacagc aaacacgatc atatcggcag tgagagttgc agagatattc
     4681 cgttgtctgt cagactcttc gaaataggga gcgggattca aatcttcgct ttgagcatct
     4741 tttgcatatt cttcttgcaa ctcgcgttca aggtcatact gaccttctga agtaatataa
     4801 cgcgggctaa agaaaacaga actaatacca ctggcaatgc cccaaacttc acgttgaaaa
     4861 gcgccattac gcgcatctgt atcttggtct gctccagggt catacatcaa gttgcccaac
     4921 tcgttatagc gcacataact ggtaaaagca cgataatgct catacgcgac aacatagttg
     4981 atgatctctg ccttctgacc acgaatcgac gcatccaagc cggcaaagtc attatcacca
     5041 gcagtagata ctgcaaccga tgccagacaa gccgctgtcg ctcctagcac ggttacgatg
     5101 gcggtaagaa cagcgatcaa tgatttaaag ctatcttgtt tcataactca tctctccttt
     5161 ggctgataag acgattatgc ggcacccttc agataaatac ctgttgggtc taattcttca
     5221 cgaaggattc tcaattcctt tttagtgact tgttccgttg ttttgagagt ggatgctact
     5281 ttgagattcc agcctgtgtt ggctttcgcc tcttccgccg ttctgcctgt gtgcagcgcc
     5341 gtgagcatca tctcgcctga ttcatctggc tctagaattc cgatgtcagt tactacagcg
     5401 agcatcccgc caccgcgcat gccgcgcgct tcgcgtgctc ctttgccgtc aatgaaacca
     5461 gccgaggtca tgaactccac tttctcaggg aaacgtcgct tctggtgggg ggtcatgatg
     5521 taacagcgat ttgcccatgc cgcaatttcc tgtgaaccac ccgaacccgg taagcggact
     5581 tttggatgcg catagtcacc aattgttgtg gcattgatgt ttccatactt atcaatctgc
     5641 gcaccaccca taaaacctac atctacattg ccacgctgaa ggtaattggc aaaaatatca
     5701 tacatactca caaccgaaag cgcaccactg accagcgtcg ggtcaccgat ggagaggggc
     5761 aggcgttctg gttcagcacc gatcacgccg gcttcataga tcattacaag gttcggagca
     5821 tgggtacgct tggcaagatt acaagctaaa ttgggttgac ctacaccaac gaagaccaca
     5881 tcgccgtcgc gcaagaggcg ggcagcgttg atgatcatca gttcagcgga ggaatatttg
     5941 agttcgggca tgttgttctc ctaaattgaa tcatagacaa tggctcgcct atgatctact
     6001 gtctgtcgtc aaaaagttct taataatgtc agccacttct tttggatgtt caagcggcag
     6061 aatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcaaaaa gttcttaata
     6181 atgtcagcca cttcttttgg atgttcaagc ggcagaatgt gagtggcttt tttcatggtg
     6241 tgaatttgaa tcttcgggtt tttctttttc accaactttt cagcatttgg caggaaggta
     6301 tcggtttcat caccacgaat aatcaatgtt ggtacatcaa aatttgcaag cccgcgccag
     6361 aggtcgtaat cgcgcattcc ggtcatataa atgtgagttt cccattcagg ggtataaacg
     6421 agctcatacc cgtcgcttgc tttgggcttg gtgatgccta caatgtattg cttcaaactc
     6481 tcatcactca tatagcgaaa aacgggcttt gttcgataac tgcgaaagac actttccaaa
     6541 tctgtgaatt cacgtctgcg ccgtttggct gtttgaataa gcggatgggc tttttctcca
     6601 atccctatgg cgcggacaaa attccagata ttcattgtgc tgggaacgaa aagcacaggg
     6661 tcaagcagaa tcacagcgcg gaatcgcttt ggctcatgca tggcagctcg cagggtgaca
     6721 atccctccga tggaatgacc cactccaata acaggaccag tttcccggtc cgataagagg
     6781 cgtagtagat cttctgagta tgggtgccaa tcgtgcaggt catccatatt ggcgccatcc
     6841 catagtggac ggagtttcat tccaaagaga tgatagttat gttttaaatg ttccagtagt
     6901 gatgtgtaac attctggcgg gtagccattg gcatgaagaa aatgcagcgg ttcaccattg
     6961 ccgccaaaat caaaatgtgg aattggattc acgttagtac ttgccgtaat ttattttttc
     7021 actatattgt gctttaactt tcaaacgctt gtgtgtcttt tcgccgagct ttttccaata
     7081 ggcattgcgg tcttttacgc catacaccca ttcatcaaga tatttttgag tcagctcttt
     7141 tgattcgctg atcttatccc acgcaaggta gaactcattg tcgcgatcgt aataaccttg
     7201 cgaatacgat ggatgactcg cgaacggaac gtgacatacc gcgctgacga caatgccggg
     7261 aatcatcgtg cggttcgggt cagaccggat aactgattcg tcgacaattt cttcagcggt
     7321 aaggatgact tttttcgcgg cgaaagcggc ttctttctgt tcgccgatga tgccccaaag
     7381 atgggcattg ccattcttat ctgcgcgctg tacgtggaca atagcaacat ctggattcaa
     7441 ggctggcacg acaatcacat ctttgccgcc gtatgggtcg gggatgcgct tgatctgcgg
     7501 attgaccttt tccaagtctt ctgcaccggt ttgattcatg ggcaggaatg gcaacccaga
     7561 tgcacctgcc tgtaagcgag taatcatgcc gaagtgagaa tattcttccc attccaactc
     7621 tccacgctca atggcgccac gcacgatgcg taacgaacca acgccgggat tccccatgta
     7681 ggagaaaatc actttcttgg cacaacccgc cgcaaccagt tgatcgtaga tcaggtctgg
     7741 agtggcacga gcaagcgtga gattcttttt gccctgacga atgatctcat gcccggcagc
     7801 gaatgggatg aggtgtgtga acccggcagc gtagaccacg tcgccatcac taacgtattc
     7861 tgcaattgct tttttgagtg gaatcagttt tgacataaag aaagtatgga ttatcctttt
     7921 ttatcttttc cttcaccgcc accgccgcct tttctacccc agcgtgtgtt tccccaaaag
     7981 cctttgaacc attcaaaacg cccagaaggg ggaggtgg
//
LOCUS       DAOQNJ010000049         7975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4653_length_7975_cov_9.976768, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000049 DAOQNJ010000000
VERSION     DAOQNJ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4653_length_7975_cov_9.976768"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1420)
                     /locus_tag="PLG52_02565"
     CDS             complement(131..1420)
                     /locus_tag="PLG52_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40353.1"
                     /translation="MATAISVKNIGKQYKIGAAETKFRYNMLRDVIVDTVAAPVRLAK
                     AMIGKSDRRLNQNYVWALKDVSFDLEEGKVLGIVGRNGAGKSTLLKILSRVTDPTTGS
                     VTVRGRVGSLLEVGTGFHPELTGRENIYMNGAILGMKRSEIESKFDEIVDFSEVSQFI
                     DTPVKRYSSGMYLRLAFAVAAHLEPEILVVDEVLAVGDAEFQKKCLGKMGDVAQQGRT
                     VLFVSHNMSAILRLTQEAVVLNKGQLIMRGPTQEAVDYYLSSGQSQAGERTWDADEVP
                     AASAPFMPISLKVKERSGKIVDTIRSTEKVTVEFEYKLSSPVTGLRVGLYISTMRGEY
                     VFTSFDTDTPELYEKFDSRNAGHYISRAELPADIFNEGRYMVGVNASSFGVRRYFMDE
                     NALAFNVDISGAPGTQWGEPRVGPIRPRLNWKIEKID"
     gene            complement(1420..2265)
                     /locus_tag="PLG52_02570"
     CDS             complement(1420..2265)
                     /locus_tag="PLG52_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40354.1"
                     /translation="MTELTKHEPHTVYIKPSKGLAALNLRDLWVYRELVYFMVWRDVK
                     VKYKQTLLGMAWAVVQPVMTMLVFTFLFGKVAKLPTDGIPYPVFSFTALLPWGLFVNA
                     LNQGSRSLVAHNNMVTKIYFPRLILPMASVFSGMVDFVIAFVILVGLMFYYQVTPAWN
                     LLWTLPLFLLLALVTALGVALWLSAINVQYRDVNQALPFLSQFWLFATPVAYSSKVIS
                     EKWQVVYSLNPMAGVVNGFRWALLGTGNGPDISLWVSVGISILVFVTGLFYFKSMEKT
                     FADTI"
     gene            complement(2268..3239)
                     /locus_tag="PLG52_02575"
     CDS             complement(2268..3239)
                     /locus_tag="PLG52_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNB40355.1"
                     /translation="MAENFWSNKRVVVTGGGGFLGSFVVEKLKERGATDIFIPRKKDY
                     DLTQREAIERLYVDILKSVDPKNVVIIHLAANVGGIGANREHPAEFFYDNMIMGVELM
                     HQAYKHGVGKFVATGTVCAYPKFTPVPFKEDAIWDGYPEETNAPYGLAKKMMLVQAQT
                     YRQQYGFNAIYLLPVNLYGPRDNFNLQTSHVIPALIRKAVEAGERGDKELPAWGDGSP
                     TREFLYVEDAADGIVTAAENYNGDLPVNLGSGYEISIKDLTEMVAKLTDFQGKIVWQT
                     DKPNGQPRRGLDVSRAKELFNWSAQVSFEEGMKRTIEWFKANRDKIK"
     gene            complement(3256..4266)
                     /locus_tag="PLG52_02580"
     CDS             complement(3256..4266)
                     /locus_tag="PLG52_02580"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB40356.1"
                     /translation="MTDRHVLVTGGAGYIGSLLTSELLRQNYRVTLLDSLLFGGESIL
                     PFMSHPNFHFLKTDVTEPRAIRDAVKRDWGKPDAVVHLAGIVGFPACQAVGKQVAWKY
                     NVEATKMVYGQSADLGVERFVFASTYSNYGYSEDGKPVTEETPLHPQSLYAETKIAAE
                     EYLLSQKDSACAPLLFRFATLYGISPRTRFDLIVNQFVLEAFTKRQLIIYQRGYSRSF
                     VHIRDVVRGIIMGLEAEQNKIRGQVFNLGSDTGNYSKNDIVGFVLKRMPETIVEYKNL
                     TFGGDMRDVTVSFEKIRNVLGFETTLNVDDGVREVLYALKSGLIKDPTDDRYRNAQFI
                     VQ"
     gene            complement(4263..4739)
                     /locus_tag="PLG52_02585"
     CDS             complement(4263..4739)
                     /locus_tag="PLG52_02585"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNB40357.1"
                     /translation="MTVESTNEEMRELTLLEQIESDPDVNQATLAQQLGVAVGTVNWH
                     LKRLIAKGAVKVARAERKKLRYIITPEGLALRARLAVNYVENSFSVYRRTRQKVKDNL
                     AKVQKAGYDQIRIIGSGDVADICKLTCIEQGITVVTDKSAPALVLDGYRIRLEGLE"
     gene            4807..6240
                     /locus_tag="PLG52_02590"
     CDS             4807..6240
                     /locus_tag="PLG52_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB40358.1"
                     /translation="MLSTPSFDEAGMQYQQNRITHWNKIAQKRDSWKGLGGWYHRRLE
                     EVYKFLVAPNQRILEIGCGQGELLASLNPLKGVGVDFSEEMIVRAKQRYPNLEFHQMD
                     AHDLSHLEGKFDIIILSDTTNDLWDVQRALEQAKRFCEPHTRLILNFYSHLWQFPLTL
                     AQGLNLAAPMLYQNWLTSDDVNSLLHLAGFEMIRASNEIVWPLPLGGFANRYLVRLWP
                     FRELALSNFVIARPSAQVVEKPSVSVVVAARNEEGNIKNIFERVPQMGSKTELIFVEG
                     HSRDKTYETIEKEIALHPQTSSLLFRQPGIGKADAIRLGFEKASGDILMILDADLTVP
                     PEDLPRFYEAIASGKGEFINGVRLVYPMEKEAMRTLNFLGNKFFSMAFSWLLGQSVKD
                     TLCGTKVLWRKDYEEIKANRSYFGDFDPFGDFDLIFGAAKLNLKIVDLPIRYRERTYG
                     TTNISRWKHGVLLLRMVVFAARRIKFI"
     gene            6253..6903
                     /locus_tag="PLG52_02595"
     CDS             6253..6903
                     /locus_tag="PLG52_02595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB40359.1"
                     /translation="MMNSHLRLLPYKEYKGVEKIYSVIFYYLPIFGRMYRKRVELCLN
                     QCKGGESILEVGFGSGLTFINLSEKYTKIYGLDLTCDVKVVSKVFEPHKIQPDLQNGN
                     VLSMPYEDSKFDTVLLISILEHLKPIELEQAFKEIKRVLKPGGQVVYGVPVERPFMVF
                     MFRLLGYNIREHHFSTEIEVRETAEKILKKNHIHTMEASPVIFGSVYEIGHFTKVN"
     gene            6906..7649
                     /locus_tag="PLG52_02600"
     CDS             6906..7649
                     /locus_tag="PLG52_02600"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB40360.1"
                     /translation="MGIERHKDLIDINQKLWHRKPILRTVYTDLYRIMQDYLTQVPGK
                     NLELGSGMGTIKEVMPDFLLTDLFHYPWLDLVENAYSLSFQNASISNLLMVDVFHHLR
                     YPGNALREFFRVLVPGGRLILMEPGLGLMGRVIYEWMHIEPVGKASNITWLAPEKWSP
                     NEMDYYAAQGNATWIFKKNLFSSHLSEWEIIETKNISALAYAASGGYSGPQLYPSRAY
                     PFIRSLERFLGLFPSIFTTRLLIVLEKRF"
     gene            complement(7674..>7975)
                     /locus_tag="PLG52_02605"
     CDS             complement(7674..>7975)
                     /locus_tag="PLG52_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40361.1"
                     /translation="EGDTVPTGGRFEYNLGEQGLLERDTDYFVIDSFTYDRFYVDSIC
                     DTTPVECEFFLSLLDNKVQTYQLLKSFTYELPTYLPDINLTAVNPKIYIYERVIE"
BASE COUNT         2240 a         1834 c         1740 g         2161 t
ORIGIN      
        1 cgcggacgat tcttcgagcg aagcatccaa tcgatttcgg cagtgaaggg caggtcgccc
       61 aaaatttcgc ggagtgggtt aggttttggc atgaattttc aaaaggtcat ttttgtaagg
      121 tgacctgcga ttaatctatc ttctcaatct tccaattcaa tcgtgggcgt atcggtccca
      181 cacgcggctc gccccattgc gtaccaggag cgccagaaat atccacattg aacgccaacg
      241 cattctcgtc catgaaatag cggcgcacac caaatgagct cgcattcacg ccaaccatat
      301 aacgaccttc gttgaaaata tcggcgggca attccgcgcg gctgatgtaa tgacccgcat
      361 tacgcgagtc aaatttttca taaagttcag gggtgtcggt atcgaatgaa gtaaacacat
      421 actcgccgcg catggtgcta atatacagac ccacacgcaa accagtcaca ggcgaggata
      481 acttgtactc aaactcaacc gtgacttttt ctgtagagcg gatggtatcg acgatctttc
      541 cgcttctttc tttaactttg aggctgatcg gcataaaagg cgcactcgcc gctggaacct
      601 catcggcatc ccaagtgcgt tctccggctt gagattgtcc cgaggagagg tagtaatcta
      661 ccgcctcttg agtaggtcca cgcatgataa gttgaccctt gttcaacaca accgcttctt
      721 gcgtaaggcg caaaatcgca gacatattat ggctgacgaa caaaacagtg cgtccctgct
      781 gagcaacatc acccatcttt ccaagacatt tcttttggaa ttcggcatcg cccacagcaa
      841 gcacttcatc caccacaaga atttcaggtt cgagatgcgc cgctacggca aatgccaaac
      901 gcaagtacat acccgatgaa tatcgcttca caggtgtatc gatgaactgg ctcacttcag
      961 agaaatccac gatctcatcg aactttgatt cgatctcaga gcgtttcata ccgaggatgg
     1021 cgccgttcat gtagatattc tcacgcccgg tcaattcagg gtgaaagccc gttcctacct
     1081 caagcaggga gccaacccgt ccgcgcacgg tgacagaacc agtggtcgga tcagtgacgc
     1141 gggaaaggat tttcagcaag gtagatttac cagcaccgtt acgccccaca atgccaagca
     1201 ctttgccttc ttcgaggtca aaggaaacat ctttcaatgc ccagacataa ttctggttca
     1261 ggcggcggtc agactttccg atcatagcct tcgccaaacg cacgggcgct gcaaccgtat
     1321 ccacgatcac atcacgcaac atgttatagc ggaatttggt ttccgccgcg ccgatcttgt
     1381 attgcttacc aatatttttt actgaaatcg ctgttgccat tagatcgtat ccgcaaaggt
     1441 cttttccata cttttgaagt agaagagacc cgtcacaaaa accaaaattg aaattccaac
     1501 agaaacccac aacgagatat ccggtccatt gcctgtaccg agcaatgccc aacggaaacc
     1561 gttgaccaca cccgccatcg ggtttaggga atagacaacc tgccacttct ctgaaatgac
     1621 cttggaggaa tatgccactg gtgtagcaaa caaccagaac tgcgaaagga agggaagcgc
     1681 ctgattcacg tcacgatatt gaacgttaat agcagaaagc caaagcgcca ctccaagtgc
     1741 ggtcacaaga gcaagcaaga ggaagagtgg aagcgtccag agtaaattcc aggcaggggt
     1801 aacttgatag tagaacatca agccaaccaa gatgacaaag gcaatcacaa aatccaccat
     1861 gcccgaaaag acagaagcca tcggcagaat cagacgcgga aaataaatct tcgtcaccat
     1921 gttattgtgc gccacaagcg aacggctgcc ttggttaagt gcgttgacga atagtcccca
     1981 cggcaaaagg gcggtaaacg aaaagaccgg gtaaggaatg ccgtctgtag gtaatttagc
     2041 gacttttcca aagaggaaag tgaagaccaa catggtcatc accggttgga caactgccca
     2101 agccatacca agcaacgtct gcttgtattt cactttgaca tcacgccaaa ccatgaaata
     2161 aaccaattcg cgatacaccc aaaggtctcg caagttgagt gccgcaagcc ctttagacgg
     2221 cttgatataa acagtatgag gttcgtgttt tgtaagttca gtcatattta cttgatctta
     2281 tcgcgattgg ctttgaacca ttcgatggtg cgcttcatgc cttcttcaaa tgaaacttgt
     2341 gcgctccagt tgaagagttc ttttgcccgc gagacgtcaa gtccgcggcg gggctgacca
     2401 tttggcttat ctgtctgcca gacgattttg ccttggaagt cggtgagttt tgcaaccatc
     2461 tcggtgaggt ctttgataga aatttcatac ccagaaccaa ggttgaccgg caaatcgccg
     2521 ttgtagtttt cggcagcagt cacaatgccg tcggcggcat cttccacata gagaaattca
     2581 cgagtgggtg aaccatcgcc ccaagcgggg agttctttgt caccacgttc gccagcttcc
     2641 actgccttgc gaataagcgc agggatgaca tgtgaggtct gcaaattaaa attatcgcgc
     2701 ggaccataga ggttcaccgg caaaagataa atggcattga agccatattg ctggcggtag
     2761 gtttgagctt gtaccagcat catcttcttg gcaagcccgt aaggagcgtt ggtctcttcg
     2821 ggataaccat cccaaatagc atcttcttta aatggcacag gcgtgaactt ggggtatgcg
     2881 cataccgtcc cagtggcaac aaacttaccc acaccatgct tgtaggcttg gtgcatgagt
     2941 tccacaccca tgatcatgtt gtcgtagaaa aattcagccg ggtgttcacg attcgcaccg
     3001 atgccgccta cattcgcagc aaggtgaata atgactacat tttttgggtc tacgcttttg
     3061 aggatatcca catataagcg ttcgatggct tcgcgttggg ttagatcgta gtcttttttg
     3121 cgcggaataa aaatgtccgt cgcgccgcgc tccttcaatt tctccactac gaacgagccg
     3181 aggaaaccgc ctccgcctgt gacaactaca cgtttattac tccagaaatt ttctgccatg
     3241 ttttatatct ctccattatt gcacaatgaa ttgtgcattt ctgtatcggt catcggttgg
     3301 gtctttgatc aacccagatt tcaaagcgta taacacttcg cgcacaccat catctacatt
     3361 caaggtggtt tcaaagccaa gcacattgcg aatcttctca aacgaaacgg tcacatcacg
     3421 catatcgcca ccgaaggtca ggtttttata ttcaacaatc gtttcaggca tccgttttag
     3481 cacaaagcct acaatgtcat ttttggaata atttccagta tctgagccaa ggttgaagac
     3541 ctgtccgcga atcttgtttt gctctgcttc gagtcccata atgatgccac gcaccacatc
     3601 gcggatatgc acaaatgagc gggagtatcc gcgttggtag atgatgagtt ggcgtttggt
     3661 gaacgcctca agtacaaatt gattaacgat taaatcaaag cgggtgcggg gtgaaattcc
     3721 atacaatgtt gcgaagcgaa agagcaaggg tgcacaagca gaatcttttt gggaaagcaa
     3781 atactcttca gcagcgattt tcgtttcggc gtaaagtgat tgaggatgaa gcggagtctc
     3841 ttcggtaacg ggcttgccat cttctgagta accatagtta ctataggtgg atgcgaacac
     3901 gaatctttcc acgcctaggt ctgcagactg tccatagacc attttcgtcg cctcaacgtt
     3961 atacttccac gccacctgct tgcccaccgc ctgacatgcc gggaagccaa caatccctgc
     4021 taggtgaaca acagcatctg gcttgcccca gtcacgcttt acggcgtctc gaatggcacg
     4081 aggctcggtc acatcggtct taagaaaatg aaaattcggg tggctcatga acggcaaaat
     4141 actttcgccg ccaaagagca aagagtcaag gagtgtgacc cgataatttt ggcggagcag
     4201 ttccgaggtc aagagagagc cgatataccc cgctcctccc gtaaccagga catgacggtc
     4261 cgtcattcca agccttccag tctgattcgg tagccatcca atacaagagc cggtgccgat
     4321 ttatcagtga caaccgtaat accttgctca atgcaggtta atttacagat atctgccacg
     4381 tcgcccgagc cgatgatccg gatttggtcg tagcccgctt tttgaacttt agcgagatta
     4441 tccttcacct tttggcgggt gcggcgatat accgaaaaag aattctcgac ataattcact
     4501 gcaaggcggg cgcgcaacgc aagcccttca ggcgtgataa tataacggag ttttttacgc
     4561 tcggcgcgag ctactttaac tgcccctttt gcaatgaggc gctttaagtg ccaattaacc
     4621 gtgccaacgg ctactcccaa ttgttgcgca agggtagctt ggttgacatc ggggtcactt
     4681 tcgatttgtt cgagaagggt gagttctcgc atttcttcat ttgtagattc aacagtcatg
     4741 atttataatt ccaaaattca gcgactgaat tataacccaa tttttggaca gagacgaatc
     4801 ccaccaatgt tatcaactcc ctcttttgac gaagctggaa tgcaatacca gcaaaaccga
     4861 attactcact ggaacaagat cgcccaaaaa cgcgactctt ggaaaggttt aggtggctgg
     4921 tatcaccgcc gccttgaaga agtatataaa tttctagttg ctccaaacca gcgcatcctc
     4981 gaaataggct gtggacaagg tgaattgctt gcatccttaa acccgttaaa aggtgtaggc
     5041 gttgactttt ctgaagaaat gattgtacgt gctaagcaac gttacccaaa tcttgagttc
     5101 catcagatgg atgcacacga cctttctcac cttgaaggca agttcgacat cattattctt
     5161 tcagatacta caaatgattt atgggatgtt cagcgtgctt tagaacaagc caagagattt
     5221 tgtgagcctc acacccgtct cattttgaat ttttacagcc atctttggca atttccatta
     5281 accttggcac aaggtctgaa tttggctgct cccatgctat accaaaactg gctcacgtcg
     5341 gatgatgtaa atagcctctt acaccttgca ggctttgaaa tgattcgagc atccaatgaa
     5401 attgtttggc ctttgccact tggagggttt gctaatcgtt atttagtccg cctctggcct
     5461 tttcgtgaac tggcactatc aaactttgta attgcacgtc cctcagcaca ggtcgtagaa
     5521 aagccaagtg tttcggttgt tgttgccgct cgcaacgagg agggcaatat taagaatatc
     5581 ttcgaacggg tacctcaaat ggggtcaaaa accgaattaa tctttgttga agggcattca
     5641 cgtgataaga cttatgaaac tatcgaaaaa gagatagcgc ttcatcctca aacctcaagt
     5701 ttgctctttc gtcaaccagg cattggcaaa gcggatgcta ttcgtctcgg ctttgaaaag
     5761 gcatctgggg atattcttat gattttagat gctgacttga ccgttccacc ggaagatttg
     5821 ccgcgctttt acgaagccat tgcttcggga aaaggggaat tcattaatgg ggtacgactt
     5881 gtttatccca tggaaaaaga agccatgcgt actttaaatt ttctagggaa taaattcttc
     5941 agtatggcat tctcatggct acttggacag tctgtcaaag atacgctttg tggcacaaaa
     6001 gttctttggc gaaaagacta tgaagagata aaagccaacc gttcttattt tggtgatttt
     6061 gacccctttg gcgactttga cttaattttt ggggcagcta agttaaattt aaaaattgta
     6121 gacctcccca ttcgttaccg cgagcgcact tatggaacaa ccaatatttc ccgttggaaa
     6181 catggtgttc tcttattaag aatggtagta tttgctgcac gtagaattaa attcatataa
     6241 aataggaaac taatgatgaa ctcacatctt cgtttattac cttacaaaga atacaaggga
     6301 gtggagaaaa tctactcagt cattttttac tatttaccaa tttttgggag aatgtaccgt
     6361 aaacgagttg aactttgcct taatcaatgt aaaggtgggg aaagcatcct tgaagtaggt
     6421 ttcggaagcg gactaacttt cataaatctt agtgaaaaat acacaaagat ttatgggctt
     6481 gacctaactt gtgatgtcaa agttgtcagc aaagtctttg agccacataa aattcagcca
     6541 gatctacaga atggcaatgt cctttcaatg ccatatgaag acagcaaatt tgacacagtt
     6601 ttactcatca gtattcttga acacttaaaa cctatagaac ttgaacaggc atttaaagag
     6661 attaagcgcg ttttgaagcc aggcgggcag gttgtttatg gagtgccggt tgaacgtcct
     6721 tttatggtat ttatgtttcg tctattaggg tataacattc gcgaacatca tttttctact
     6781 gaaatcgaag ttcgtgaaac agcagaaaaa atcctaaaga aaaaccatat ccacacaatg
     6841 gaagcatctc ctgtaatatt tgggagcgtt tatgaaattg ggcactttac caaggttaat
     6901 taatcatggg gattgagcgc cataaagatc ttatcgatat aaaccagaag ctttggcatc
     6961 gaaagcccat attaagaacc gtatatacag atctttaccg tatcatgcaa gactatctta
     7021 cccaagttcc aggtaaaaat ttagagcttg gttctggtat gggcacaatt aaagaagtta
     7081 tgccagattt tttacttaca gatctttttc actatccttg gttagattta gtagaaaatg
     7141 cttattcatt atcttttcag aatgcatcaa tatcaaactt actcatggta gatgtttttc
     7201 atcatctgcg atatcctggg aatgctttaa gagagttctt cagagtatta gttccaggtg
     7261 gtcgtcttat actaatggag cccggtcttg ggctaatggg gagagttata tatgaatgga
     7321 tgcatattga accagtagga aaggcaagca atataacctg gcttgcccca gaaaaatggt
     7381 ctccaaatga aatggattat tatgctgcac agggcaatgc tacttggata tttaaaaaaa
     7441 acctattttc atcacatctt agtgaatggg aaatcataga aacaaagaat atctcagcac
     7501 tagcatatgc tgcttctggc ggatattctg gtccacaact ttatccttca agagcatacc
     7561 ctttcatcag gtcacttgaa aggttcttag ggctttttcc ttctattttc acaacacggt
     7621 tattaattgt tttagaaaag aggttctgaa gcatacttca gaacctcttt tctttattca
     7681 attactcttt catatatgta aattttggga ttaactgctg tcaagtttat atcaggtaag
     7741 taagttggaa gttcataggt aaagctttta agcaactggt atgtttgcac tttgttatca
     7801 agtaaactta aaaagaattc acactctact ggagtagtat cgcaaattga gtctacataa
     7861 aaacgatcat aagtaaaact atctattaca aaataatctg tatcacgttc caaaagaccc
     7921 tgctctccaa gattgtattc aaaacgtcca cctgttggga ctgtatctcc ttccc
//
LOCUS       DAOQNJ010000050         7865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4752_length_7865_cov_7.895647, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000050 DAOQNJ010000000
VERSION     DAOQNJ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4752_length_7865_cov_7.895647"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..358)
                     /locus_tag="PLG52_02610"
     CDS             complement(11..358)
                     /locus_tag="PLG52_02610"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB40362.1"
                     /translation="MVTPGLKQEITQLEANFCAALSDPIRLLILYTLHDGSRNVTDLA
                     TELGINQPTTSRHLKILRERGLVETSRNGTTITYSLSDPRLIQALDLLRSVMRERLAY
                     QANLINETVQESL"
     gene            517..1122
                     /locus_tag="PLG52_02615"
     CDS             517..1122
                     /locus_tag="PLG52_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB40363.1"
                     /translation="MEPYQREAISVDVSVRLRELREARGISMRTLASRSGLSANALSM
                     IERGKTSPSVSTLYKLANALGVSITAFFDTETEKRQVVFLKSDGRTRLSFTRGVFEAL
                     GGESFVGRVEPFMLTMESGASCGPHDIVHSGHEFVFCLRGQLDYTVEKETFRLEPGDS
                     LLFASKLRHRWKNPANVVANALIIISGFAEGEEPHSMHWNK"
     gene            complement(1185..1532)
                     /locus_tag="PLG52_02620"
     CDS             complement(1185..1532)
                     /locus_tag="PLG52_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNB40364.1"
                     /translation="MSDSQINEIQWTIHTTNPDLVKQARASLSEVVDPEIGMNIIQLG
                     LVRDIEVENGMARLKMILTTPFCPYGPAMIEMTKAKATEGLNMPVTVEMGMDVWDFSM
                     MEDPSALDWGMYA"
     gene            complement(1545..2168)
                     /locus_tag="PLG52_02625"
     CDS             complement(1545..2168)
                     /locus_tag="PLG52_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNB40365.1"
                     /translation="MKSTRDKILQTLLKKPKSTINDLADAVGINPISVRHHLTNLQME
                     GLVESQEERHGVGRPRLVYVLTDEGMERFPTRYLRLTTRLIAQMKESIPGPMVSKLFG
                     QIAEDLANEYANDIKGLSMEERLDFVKDMLAQEGFTVEWEKKDGQYQIHEISCPYYQI
                     GVAHPEVCTVDQTLISKMLALPANKVRCILDGGAHCTYVVQPVGKTK"
     gene            complement(2240..5290)
                     /locus_tag="PLG52_02630"
     CDS             complement(2240..5290)
                     /locus_tag="PLG52_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB40366.1"
                     /translation="MRTKIIKISFIGLFLFSTVFSQHPNKVHAQSDKIVFAAIGDYGL
                     AGQPLLDVSNLIKSWNPDFIVTLGDNNYPDGAAWSIDDNIGQYFHEYIFKYKGKYGEG
                     SPTRRFYPSLGNHDWTTGANAYFNFFGYYNPVTHYDFIQGPVHFFIVDSDRNEPDGVM
                     SDSAQAKWLKKTMLASTSPFNVVVFHHAPYSSGRHGSTEYMRWPFKDWGADVVLTGHD
                     HVYERLSVNGIPYFVNGIGGAEIYNFDRILPESQVRYNQDFGAMRIEATSLYMKFQMY
                     SRAGVLVDEYTIGGSNPSVSSITRLNAASTNLSGVNFQVNFSEAVTGVDVSDFLLTTN
                     LSGANIASINGSGNAYTVTVNTGSGDGTLRLDLTDNDSILNSLSIPLGGSGAANGNFS
                     AGEIYYIDHTSPSVSSIATASLNPSNAATVDFAVTFSEPVSGVDLADFSVSTTNGAVL
                     TGISGSGMNYVLTVATGTGNDGLRLDFIDNDSVLDLAGNPTNVGFTSGGSYSIDRTAP
                     VVTSITPLSQTNETSVDYAVSFSEPVTGVDGSDFFLFTMNGASITNVSGSGNQYLVSI
                     SLNPGSDSVRLDVNDNDSILDSVGNPLGGIGAFNGNFIGSTFNVAIDTPIATSIIRAS
                     NNPTNAANVNFIVTFSEPVDGVDIGDFILSPNAVITNITNSNPFYIITVNSENINGEL
                     KLQLNDDDTIHNAQGIPLGGNGIGNANFTTGETYLIDRISPQVTSIIRAGGNPAINPT
                     ADFIVTFSEPVNGVETSDFILTSSNVIQSSVVGMQNADPFYWVKVSTGIGSGSIRLGL
                     LDNGNITDKAGNPLLNRNFTGESFNIAKTPVDFSAPTMNSVQGKLTNNPLVSISWSAV
                     RNAQAYEVFVARDSNFSQIVFIQTTTSTSITPPTPLADGGYYVRVRAYNPDLNPGKFS
                     KAINFAVDATPPVAPNLLSPASGATTTKRAWLKWSVVNSVVKYQIQVDNNSDFSSPEF
                     DATTSKATIQTKSLPARTYYWRVRGIDSAGNWSAWSQTFLFTTK"
     gene            5404..6357
                     /locus_tag="PLG52_02635"
     CDS             5404..6357
                     /locus_tag="PLG52_02635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNB40367.1"
                     /translation="MKRVFYFVFALLIVACNLPATENSTSEPVNTATLELPTATPSLA
                     PSATATLIPPTLTATPVPCDPLAVDYCITSGHFLFQRPIHLPDNETVDLTYLYASTSN
                     GKRDPHHGVEYQNPFGTSVFAAGDGEVVFADADKKTKFSPWNNFYGNVIVIRHADEMY
                     TLYAHLSEILVQVGDQVQAGDEIGKVGQTGGATGSHLHFEVRKGGDYTDYFSTENPEL
                     WTLPPEGTGALSITLKTTEERNYERQLVITRLEEDSQDILFTYYITTYAKGFEHNIED
                     AGLGNLPAGQYKIAFTGPTGFVERLVLIEAGHLTEVVFDLQ"
     gene            complement(6409..6996)
                     /locus_tag="PLG52_02640"
     CDS             complement(6409..6996)
                     /locus_tag="PLG52_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40368.1"
                     /translation="MKKFFANEIFLGTMIAVLSVFTAYVSYEGAMADSKQNEFEIKGM
                     KDLNDGNAEYLRANQDITQDYNYFDNWYINDGTDRQDIADYYKENFSEALQTAFDSEE
                     GVWSEAYYDAMYADAYAFWDSSDANFETGSQYDERGDQLQLVMLVTALGLAFVAWASL
                     LGAESNMRVLFAVFGTIALIAGVLVFFLMVPTVAG"
     gene            complement(7152..>7865)
                     /locus_tag="PLG52_02645"
     CDS             complement(7152..>7865)
                     /locus_tag="PLG52_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB40369.1"
                     /translation="WKWYHRVIGKEKCPIIDTWWQTETGAFQITPTPVVPLKPGSGTR
                     PFFGQKAEIVDEQGNPVPDDTEGYLTLLRPWPSMLRTIYGDDERYVNQYWSKYPGRYT
                     TGDSAKRDTDGYYWIIGRVDDVIKVSGHRLGTAEVESALVSHPAVAEAAAIGLPHDVK
                     GQAIYTFVILRAGFAPSPELSEELRQHVSKHMGPIARPEDVKFLDKLPKTRSGKIMRR
                     VLKARAQGLPEGDISTLEE"
BASE COUNT         2009 a         1762 c         1924 g         2170 t
ORIGIN      
        1 aagcgtttag tcatagagac tcctgtacag tttcattgat caggttcgct tgataagcga
       61 gtcgctcacg cataacgctg cgcaacaggt caagtgcctg aataagacga gggtcagata
      121 agctgtaagt aatggtggtt ccattgcgag aggtttctac gagaccgcgt tcgcgcagga
      181 tcttgaggtg acgagaggtg gttggctggt tgatgccaag ctctgttgcc agatctgtga
      241 cattgcgaga accatcatgc agggtatata aaatgaggag ccgaatgggg tctgaaagag
      301 cagcgcaaaa attggcttca agctgggtaa tctcttgttt taaaccgggg gtaaccataa
      361 acaccttctt atatgcatat aaacgaatat ttatagttgt atcataatat tgtgacagat
      421 tgcacaatag tggagggtgt catattaatt ttcgtttaat gtcacatata attggtgtat
      481 aatttttgca gtttaaagaa gcttttggag atagaaatgg aaccttatca acgggaagca
      541 atttctgtag atgtatctgt tcgattgcgt gagttacgcg aggcgcgtgg catctcaatg
      601 cgcaccctgg cttctcgaag cgggctttct gcaaatgcat tgtccatgat cgagcggggg
      661 aagacctcac catctgtgag cactttgtat aagttagcga atgcattggg tgtttcaatt
      721 actgcgtttt tcgataccga gaccgaaaag agacaagttg tttttctgaa gagcgatgga
      781 cgcacccgtt tatctttcac tcggggcgtg tttgaagctc tcggcggcga aagctttgtt
      841 gggcgggtgg agccttttat gctgacgatg gaaagtggcg cttcctgcgg tccgcatgat
      901 attgtccatt cggggcatga attcgttttt tgtctgcgtg ggcaattaga ttacacggtg
      961 gaaaaagaaa ccttccggct cgaaccgggg gatagcttgc tcttcgcttc caagcttcgc
     1021 catcgctgga agaatcctgc taatgtcgtt gcgaatgctt tgattattat ctctggcttc
     1081 gctgaaggcg aagagcctca ctctatgcat tggaataaat aaattaagat gttaaataaa
     1141 aagagccacc tgtacaggtg gctctttttg ctcttttact aaaactacgc gtacataccc
     1201 cagtcaagtg cagaggggtc ttccatcatg gagaagtccc acacatccat gcccatttct
     1261 acagtgacgg gcatgttcaa gccttcagtg gccttggctt tggtcatttc gatcatagca
     1321 ggaccatacg ggcagaaggg cgtggtcagg atcattttga ggcgcgccat accgttttcc
     1381 acttcgatat cacgtaccaa gccaagttgg atgatgttca taccaatttc agggtccact
     1441 acttcagaca aactagcgcg tgcctgcttg actaggtctg ggttggtggt gtggatggtc
     1501 cactggattt cgttaatttg agaatcgctc ataaagtcct ctttttattt tgttttaccg
     1561 acgggttgaa ccacatacgt acaatgagca ccgccatcta aaatgcagcg aactttgttt
     1621 gcaggcagtg ccagcatttt ggagatcaga gtttgatcta ccgtgcagac ttctgggtgt
     1681 gccacgccga tctgatagta tgggcaggag atttcgtgga tctggtattg tccatctttc
     1741 ttttcccatt ccacagtaaa accttcctgc gccaacatat ctttgacgaa gtcaagacgc
     1801 tcttccatac ttagaccttt gatatcgttg gcatattcat tggctaggtc ttcagcaatc
     1861 tgaccgaaca acttgctcac catgggtcca gggatgcttt ctttcatctg agcaatgagg
     1921 cgggttgtca gacgcaggta gcgggtaggg aagcgttcca tgccttcatc ggtcagtaca
     1981 tagaccaaac gtgggcgtcc taccccgtgg cgttcctctt gcgattcaac cagcccttcc
     2041 atttgcaggt tggtgaggtg atgccgcaca gagatcgggt tgatgccgac ggcatctgcc
     2101 agatcattga tggtagattt gggtttctta agtaaagttt gtaaaatctt gtctcgcgta
     2161 gatttcatgc agggcagtat aacttccctg taggctcttg tcaatattct agataaatat
     2221 cataaaaatt acgagagggc tattttgtgg tgaataggaa ggtttgagac caggcgctcc
     2281 aattcccagc actgtctatg ccccgaactc tccaatagta agttctagct ggcaggcttt
     2341 ttgtttgtat tgttgccttg ctggtcgttg cgtcgaattc agggcttgaa aaatcggagt
     2401 tgttgtccac ttgaatttga tatttcacaa cgctgtttac tacagaccat ttcaaccatg
     2461 cccgtttggt tgtggttgca ccagaggctg ggctaagaag gttgggggca acgggcggag
     2521 ttgcatctac tgcaaagttg attgctttgc tgaatttccc ggggtttagg tctgggttgt
     2581 aagcgcggac tcgtacataa tagccgccat ctgcaagtgg agtaggcgga gttatggatg
     2641 tagaggttgt tgtttgaata aaaacaattt gtgaaaaatt actatcgcgt gcaacgaata
     2701 cttcatatgc ctgtgcatta cgcaccgcag accatgatat agagactaat gggttgttgg
     2761 tcaatttgcc ttgaacggaa ttcatggtgg gtgccgaaaa atccacaggg gttttggcaa
     2821 tattgaaact ctcgcctgtg aaattcctat tcaggagtgg gtttccggct ttgtctgtaa
     2881 tgtttccatt atccaatagg ccgagccgaa tgcttcccga gccgatccct gtactgacct
     2941 tgacccaata aaatgggtct gcgttttgca tgcccaccac actcgattga atgacattgg
     3001 aactggtcaa aataaagtca cttgtttcca cgccgttcac gggctccgaa aatgtgacga
     3061 tgaaatctgc cgttgggttg atagcgggat taccacctgc gcgaatgatt gatgtcactt
     3121 gcggcgatat acggtcaatg agataagttt cacccgttgt gaaatttgca ttccctatcc
     3181 cgttcccgcc gagtggaatg ccttgggcat tatgaatggt atcgtcatcg ttcagttgaa
     3241 gctttagctc gccattaata ttttctgagt taaccgtaat aatgtaaaac ggattcgagt
     3301 tcgtgatatt ggtaataaca gcattgggtg acaaaataaa atcgcctata tccacaccgt
     3361 caaccggctc agagaaagtg acgataaagt tcacgtttgc ggcatttgtt gggttgttgc
     3421 tggcgcgaat gatcgatgtc gcaatcggcg tgtcaattgc aacattgaac gtgctgccaa
     3481 tgaagtttcc attgaacgcc ccgatccctc ctagcgggtt accgacacta tccagaatgc
     3541 tatcgttgtc atttacatct aagcggacgc tgtcgcttcc ggggttgagc gagatggaaa
     3601 caagatattg attcccggaa ccagagacat tcgtaatgct tgctccgttc atagtgaaga
     3661 ggaagaaatc gcttccatcc acgccggtga ctggttcaga aaaactgacg gcgtaatcta
     3721 cgctggtttc gttcgtttgg ctaagcggtg tgatggatgt aaccacaggt gcagtccggt
     3781 caatactata agaccctcca cttgtgaagc caacattggt ggggtttcct gccaagtcta
     3841 ggacgctatc gttatcaata aagtctaggc gcagaccgtc attcccagtc cctgtcgcca
     3901 ctgtgaggac gtaattcatg ccagacccag aaatgccggt taatactgcg ccattcgtgg
     3961 tggatacact gaaatccgcg aggtctacgc cagagacagg ttcagagaat gtgacagcaa
     4021 agtccacagt ggcggcattg ctggggttga ggcttgctgt ggcaatcgaa ctcacagatg
     4081 gagatgtgtg gtcaatgtaa tagatttcgc ctgccgagaa atttccgttt gcagcgccag
     4141 agccgcctaa gggaatgcta aggctgttta agatagagtc gttatcggtt aggtcgagcc
     4201 gtagggttcc atccccagac cctgtgttga ccgttactgt ataggcgttg ccacttccat
     4261 ttatgctggc gatatttgct ccactgagat tggttgtaag aaggaaatca gaaacatcta
     4321 cgcctgtaac agcttctgaa aaattcacct gaaaattaac tccactcaga tttgtggatg
     4381 ctgcattaag ccgcgtgata gatgaaacag aaggattact gccaccaatt gtatattcat
     4441 cgacaaggac gccagcgcgc gaatacattt ggaatttcat gtataggctt gtggcttcta
     4501 ttcgcatggc tccgaaatct tgattgtagc gaacctggct ttcaggcaga attctgtcaa
     4561 aattatagat ttcagcgccg ccaatgccat ttacaaagta tggaatgcca tttacggaaa
     4621 gccgctcata cacatgatcg tgccctgtaa ggacaacgtc tgcgccccaa tctttgaacg
     4681 gccagcgcat atattcagta gacccatgcc gcccggaaga ataaggggca tgatgaaaaa
     4741 ctaccacatt gaacggcgat gtggatgcga gcattgtctt tttcagccac tttgcctgtg
     4801 ccgagtcaga catgacgcca tctggttcgt tacggtcgct atctacgata aagaagtgaa
     4861 ccggtccttg gataaagtcg tagtgtgtga cggggttgta atacccaaag aagttgaagt
     4921 aggcgtttgc gccggttgtc caatcgtgat tgccaagcga tggataaaaa cgacgagttg
     4981 gtgagccttc gccatatttg cctttgtatt tgaaaatata ttcgtgaaag tattgcccga
     5041 tattatcgtc aatagaccat gcggcaccat caggataatt gttatcaccc aaggtcacaa
     5101 taaaatctgg attccagctt ttgattaagt tggaaacatc caaaagaggc tgcccagcca
     5161 aaccataatc accaatggca gcgaagacga ttttgtcgct ttgcgcgtgg actttattgg
     5221 ggtgttgtga aaagaccgtg gaaaaaagga ataaaccgat aaatgaaatt ttgatgatct
     5281 tggttcgcat gtctttctcc tgcctgatga ggcgacttca tcattatatt tttcgcttcg
     5341 aaaatcccct tggctggtat gaaattaata tgcttttatt cctgatgtaa aatggaaacc
     5401 actatgaaac gagtttttta ttttgttttt gctttgttaa ttgttgcttg taatttgcct
     5461 gctacagaaa attcaacatc tgaacctgtc aacacagcca cactggaatt gccaacagca
     5521 actccctctc ttgcgccttc tgccacagca acgctcattc caccgactct tactgctact
     5581 cccgtcccat gcgacccgtt agctgtcgat tactgcatca caagcggaca tttccttttt
     5641 cagcgcccaa ttcacttgcc tgataatgaa acagtcgatt taacttatct ttacgcttct
     5701 acatcaaacg gaaaacgtga cccgcatcat ggtgtggaat atcaaaaccc atttggtact
     5761 tctgtttttg cagcgggaga tggggaagtg gtctttgcag atgccgataa aaagacaaaa
     5821 ttcagcccat ggaataattt ctatggcaat gttattgtca ttcgccatgc agacgaaatg
     5881 tacactctgt atgctcattt gtctgagata ttggttcagg ttggggatca agttcaagct
     5941 ggagacgaaa ttgggaaagt tgggcaaaca ggcggggcaa ccggaagtca tctccatttt
     6001 gaggtccgca aaggcggcga ctacaccgat tatttttcca ccgaaaaccc agagctatgg
     6061 accctcccgc cagaaggtac aggtgctttg tccattacat tgaagacaac agaagaacgg
     6121 aattacgaac gtcaacttgt gattacgcgt ttggaagagg acagtcagga tattctcttt
     6181 acgtactata taacaacata tgccaaaggg tttgaacata atatagaaga tgccggttta
     6241 ggaaaccttc ctgccggtca atataaaatc gcgtttacgg gtccaacagg ttttgtagaa
     6301 cgcctcgtct tgatagaagc ggggcatctc acagaagtag tttttgacct tcagtagttt
     6361 aaaataaaaa gcgcatccat ttggatgcgc tttttatttt ttgctcaatt atccagcgac
     6421 ggtaggaacc atcaagaaga acacaagaac gcctgcaata agagcgattg ttccaaacac
     6481 agcgaacaga acgcgcatgt tgctttcagc acccaacaaa gaagcccaag ccacaaacgc
     6541 caagccgaga gcggtcacca gcatcacgag ctgaagctga tcgccgcgtt cgtcatactg
     6601 tgaaccggtc tcaaaatttg catcagagct atcccagaag gcataggcat cggcgtacat
     6661 ggcgtcataa tatgcttcgc tccacacacc ttcttcgcta tcgaaagcgg tctgaagggc
     6721 ttcagagaaa ttctctttgt agtagtcagc aatatcctgg cggtcggtgc catcgttgat
     6781 gtaccagttg tcaaaatagt tgtaatcctg ggtaatgtcc tgattggcgc gcaagtattc
     6841 ggcgttgccg tcattcaagt ctttcatacc cttgatctcg aattcattct gcttcgagtc
     6901 agccatggcg ccttcgtagc tcacatacgc agtgaacacc gaaaggacgg cgatcatggt
     6961 tcccaaaaat atttcgttcg caaaaaactt tttcatattc tctccttttt gaaaaaaata
     7021 atacaagcgc cgcgattata tacggaaaaa tttatcttaa ggtttacagt tttgttgtaa
     7081 aaaacagacc tccgaaattc gaaggtctgt tttgtggata aaattccggt tcagactcag
     7141 ccgtaacctg gttactcttc cagtgtgctg atatctcctt cgggcagacc ttgtgcgcgg
     7201 gcttttagca cgcggcgcat gattttacca gaacgtgtct tgggcagctt atcaaggaac
     7261 ttgacatctt ccgggcgggc aattggaccc atgtgtttgg atacatgttg acgcagctct
     7321 tcagagagtt ctggagaagg cgcgaacccg gcacgcaaga tgacgaaggt ataaattgcc
     7381 tgacctttaa catcgtgcgg caaaccgatg gcggcagctt ctgccacagc ggggtgacta
     7441 accaacgctg attcaacttc tgccgttcca aggcggtgac ccgagacctt gatgacatcg
     7501 tccacacgcc caatgatcca gtagtagccg tctgtatcac gtttggcaga gtcgccggta
     7561 gtgtagcgac cggggtactt gctccaatat tgattcacat agcgctcgtc atcgccataa
     7621 attgtgcgca gcatggaagg ccaggggcgt agcaaggtga gatacccttc ggtatcgtct
     7681 ggaacagggt tgccttgttc gtctacgatc tcggcttttt gaccgaagaa aggtcgggtg
     7741 ccagaacctg gtttgagagg cacaacaggc gtcggcgtaa tttggaacgc gccggtctcc
     7801 gtctgccacc acgtatcgat gatcgggcat ttctctttac cgatcacgcg gtgataccac
     7861 ttcca
//
LOCUS       DAOQNJ010000051         7767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4852_length_7767_cov_7.978864, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000051 DAOQNJ010000000
VERSION     DAOQNJ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4852_length_7767_cov_7.978864"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="PLG52_02650"
     CDS             <1..1097
                     /locus_tag="PLG52_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB40370.1"
                     /translation="TNCRAWLCRILHNTWISHTVGLEQCVAQGRAAIAWDDKYGNDNW
                     KKTASPTKRKGIGVAMVMQGTAIPYLDMGGASIKMNDDGSFNLLVGATDLGTGSDTVI
                     AQMAAEVLGVPIEDMITYSSDTDFTPFDKGAYASSTTYISGTAAVKAANIVAERIRIR
                     AATMLEVADPNSIQLKDRHAHAPNGKSVSLAEIALDTLHKNNQEQIMGVASYVSPVSP
                     PPFAAQFAEVTVDTETGQVTVDRLVMAVDSGVIVNPLTASGQIEGGMTQALGYAVCEE
                     MRYDDKGVAYERDLDRYHVFRADEMPELETIFVETFEPSHPFGVKAVAEIPMDGVAPA
                     VGNAILDAIGVNVDVNPTTPERVWKAIKRQ"
     gene            1229..2899
                     /locus_tag="PLG52_02655"
     CDS             1229..2899
                     /locus_tag="PLG52_02655"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNB40371.1"
                     /translation="MTSSELAHIIPYLASFLISIFISGYAWTKRQAQGALGFELYMVG
                     QTVWIAGFILEMISKELGDKIFWDQVQWFCTTLIFVAIPIFAIQFTEHRIYFPRRYFF
                     LSLIVPMGFSFLLATNSIHHLIYNNPSLVASTPFSELHYDFTFVIYLYGLYGGAIALW
                     SGQLVFKRLIQPQSLYRAQALIVLLGFAIPLISWTLTLLGVQITPERDSTPFSAAIGN
                     LIIAWGIFRFHIFEVIPIARDRVFEALADPVVILDKNNIVIDINAAMLTLMDKREHEV
                     IGNPAKDVFNDFPIPIKLYTHVSYARTEAVFEISGKQIHYDLTIWPLYNTNKEMVGRV
                     YVSHDITALKELEAELRKLNVQLEDRVRDRTRELAEAYESMLEGLALALELRDKETEG
                     HSRRVTENAIKLAQKMGIAGSALEDIRSGAILHDIGKISIPDEILHKPGKLTLDERAV
                     VEKHPETAFKLLSRIPFLSQAIEIPYCHHEKWDGTGYPRKLAGEDIPLSARIFAVADV
                     WDALSYDRPYNKAWTREQIIEYFNEQTGKHFDPAITKIFLSMVEKGEI"
     gene            2941..4284
                     /locus_tag="PLG52_02660"
     CDS             2941..4284
                     /locus_tag="PLG52_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNB40372.1"
                     /translation="MPDQIYVVGHINPDTDSIASAMGYAWLLRERDGLNTIAARAGAL
                     NAQTSWVLKTLGLESPFLLTDASPRFDSVMQRLDSLRPNAQLGSAWTLASRTGGLAPV
                     VNEDGTPYGIISGMSLFKYFSNILGPRPGDTTVREMMSAECKDAADTSVPKYQANAHI
                     RDSLNRILRDEIDDYWVVDESGRYLGIARQRNLLNPPRLKIILVDHNEPRQSIAALEE
                     AELLEILDHHRLGNPYTHQPIRFTVDVVGSTSTLVTEQTAEAGLSAPPAIAGALLSGL
                     LSDTLILTSPTTTDRDRVAAERLARWAFVGGSPLQGETIETYGKAVLSAGAGLSNRKP
                     EEVVSTDIKPFEGGGFKFAVAQAEVTDIMQLSEHLAPLTKALDDLRDKRGLDFALLLV
                     TDVVRGTSRLIVTSSAPPILNDLPYPPLPDGTRDAPGVVSRKKQLLPAVLGLLER"
     gene            4345..5220
                     /locus_tag="PLG52_02665"
     CDS             4345..5220
                     /locus_tag="PLG52_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB40373.1"
                     /translation="MPFPYLDETLLLNAETRKDLPGSFIALPSGVTHYEEGGDPKGQP
                     VVLVHGFSVPYFIFEPTFVFLCQQGFRVLRYDLLGRGFSDRPNTRYNATLFVHQLKEL
                     LDALNYKNICLTGLSMGGAVTSSFIDAYPDYVSKYVLIDPSGARAVSLSPFLKAVRMP
                     GFGELAIGLFGSESMLKGIASDLFSPELVREFQEKYKVQMQYQGFKRAILSTMRNGML
                     DSFIETYKRVGKLKKPTLLFWGKQDTTTPFEHSADICAVMPHAEFYAFDSCGHIPHYE
                     RASEVNRILLEFLRK"
     gene            5272..5766
                     /locus_tag="PLG52_02670"
     CDS             5272..5766
                     /locus_tag="PLG52_02670"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB40374.1"
                     /translation="MNKEYIQTLYRYNQWSNARILDTAAKLSPAQFLATSEHPHRYLR
                     GVLTHTLFAEWIWHKRWTGESPTTRLKPEDFPTFEALRTRWAEEESQLMQFVAQVTDE
                     KLNAPFTYLATDGTKYENILWESMAHVVNHGMQHRSEAAAILTELGVSPGDLDFILFT
                     RKKL"
     gene            5763..6518
                     /locus_tag="PLG52_02675"
     CDS             5763..6518
                     /locus_tag="PLG52_02675"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNB40375.1"
                     /translation="MTFNRAKNFLFRNRHNLAVFVLLNLACVACIVFVAARVVYTDSS
                     RHTGLIWNLFLAWIPFILAYFAHALSWKKVWVYLAIPFVAFLWLLFFPNAPYMLTDLQ
                     DLARGTGYEAPLWYDVIIVVWCSWTGTLLGVISLYLMQDIIARTFHRWLGWVFVFAIS
                     GLSSFGIYIGRFVRLNSWDILQNPAETAMEILGIVIDPSKRLAAFTLAYTFFFLFIYL
                     LLYSFSHMLQEQVIRAQSSPEQLASTQPTQPVH"
     gene            6585..7301
                     /locus_tag="PLG52_02680"
     CDS             6585..7301
                     /locus_tag="PLG52_02680"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNB40376.1"
                     /translation="MFQKIYNKLKPFRFRLTILGLLAVASLISIALFRFRTRISGTME
                     YDFLVWNLFLAWLPLGMSYIASSFTNKRPFVVALIVPVAAVLWLLFFPNAPYILTDLF
                     HLRHPREIVPIWYDTLLINWFAWTGVLLGVFSLFMMHDIVRRVFGRFIGWLFVLTVST
                     LCGVGIYIGRFLRWNSWDVILHPYERMREMLYYVLHPSMQSILFISVFSALFVFIYIT
                     TYTFGLLFQEQAQPISQDIS"
     gene            7298..>7767
                     /locus_tag="PLG52_02685"
     CDS             7298..>7767
                     /locus_tag="PLG52_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40377.1"
                     /translation="MNPLILIRGGGDLASGVALRLHRVGFQVAILELDKPLAVRRTVS
                     FSEAVYEGSQTVEGVTARLISADQFQVTLEAGEIPVLIDPHANILNNQFLTSPTSTFV
                     VDARLMKTEPEPLPERVALHIGLGPGFTAGANCHAVIETMRGHALGRVYWDGQTA"
BASE COUNT         1907 a         1937 c         1866 g         2057 t
ORIGIN      
        1 gcaccaactg ccgcgcctgg ttgtgccgga tcctgcacaa cacctggatc agccataccg
       61 ttgggctgga acaatgcgtg gcgcaaggtc gcgccgcgat tgcatgggat gataaatatg
      121 gcaatgacaa ttggaagaag accgcctccc ccaccaaacg caaagggatc ggcgtggcga
      181 tggtcatgca agggacagct attccttacc tcgatatggg cggtgcatcc atcaagatga
      241 atgatgacgg ttcgttcaat cttttggttg gtgcaacaga cttgggcacc ggttcagaca
      301 cggtcattgc acaaatggcg gcagaagtat tgggtgtgcc catcgaagac atgatcacgt
      361 attcatcgga cacggatttc actccgttcg ataagggcgc atatgcttcg tctacgactt
      421 atatttccgg cacggcggcg gtgaaggcag cgaacatcgt ggcggagagg attcgcatcc
      481 gcgcggcgac gatgttggaa gttgcagacc cgaactccat tcaactaaaa gaccgtcatg
      541 ctcatgcgcc gaacggaaag tctgtttcgt tggctgaaat tgcattggat accctgcaca
      601 aaaataatca agaacagatt atgggcgtgg caagttatgt ttcgcccgtt tcacctccgc
      661 cgtttgcagc acaatttgcc gaagtgaccg tggatacgga aacgggtcag gtcaccgtgg
      721 atcgtttggt gatggcagtg gactcaggcg tgatcgtgaa tccgcttacg gcttcggggc
      781 agatcgaagg tggtatgacg caggcactcg ggtacgccgt ctgcgaagag atgcgttatg
      841 acgataaggg tgtcgcctat gagcgtgacc tggaccgcta tcacgtcttc cgcgccgatg
      901 aaatgccgga gttggaaacc attttcgtcg agacctttga gccgagccat ccgttcgggg
      961 tgaaggcagt ggcggaaatt ccgatggatg gagtagctcc tgccgttggg aatgccatct
     1021 tggatgccat cggcgtcaac gtggatgtaa acccgaccac tccagagaga gtctggaagg
     1081 cgataaagcg tcagtaagaa gaaacaagca caaacgatag aatgaggcgt gaggcagtat
     1141 ttgtccacgc ctcattttct gtaaaattac aaaaataatt ttgtctcgta tatacaaaga
     1201 cagcgagata taatcaacgc cacaatacat gaccagttca gaacttgctc acatcattcc
     1261 ctatcttgct tccttcttga tatccatatt catatcaggt tatgcatgga ccaaaaggca
     1321 ggcgcaaggg gctttagggt ttgaacttta catggtgggg caaacagtat ggatcgctgg
     1381 tttcattctt gaaatgataa gcaaggaact aggggataaa attttttggg atcaagttca
     1441 atggttttgt acaacgttga tttttgttgc tatcccaatc tttgcgattc aattcacaga
     1501 gcaccgcatt tatttcccta gaagatattt cttcctatcg ctcattgtgc cgatgggttt
     1561 ctcgtttctg ttagcaacaa atagcatcca ccatttgata tacaacaatc cgtcgcttgt
     1621 agcaagtacg cctttttcgg agcttcatta cgattttaca tttgtgattt acctgtatgg
     1681 gctttatggt ggggcgattg ccctttggtc tgggcaattg gtattcaagc gtttgattca
     1741 acctcaaagc ttgtatcgcg cacaagccct gattgttctt cttggtttcg cgattccgct
     1801 tataagttgg acgctcacct tgctaggcgt tcaaatcaca cccgaaagag attcaacccc
     1861 gtttagtgct gccatcggca atttgatcat tgcctggggc atttttcgtt tccatatttt
     1921 cgaggtgatc ccgatcgcca gagacagggt ctttgaagca ttggcagacc ctgtggttat
     1981 tctcgataaa aataatattg ttattgatat caacgccgcc atgcttacct tgatggacaa
     2041 aagggaacat gaagttatcg gaaaccctgc caaagacgtt ttcaatgatt ttccgattcc
     2101 tatcaaactg tacactcatg tctcttatgc gaggacagag gctgttttcg agattagcgg
     2161 caagcaaatc cattacgatc ttacgatctg gcctttatac aacaccaata aggaaatggt
     2221 cgggcgagtt tatgtttcac atgatattac tgccttgaaa gaattggaag ctgagctgcg
     2281 aaagttaaat gtgcagttgg aagaccgtgt ccgcgaccgg acgcgcgaat tggcagaagc
     2341 gtacgaatcc atgttggagg ggctcgcgct ggcgctcgaa ttaagagaca aagaaactga
     2401 aggtcactcc aggcgtgtaa cagaaaacgc catcaagctt gcacaaaaaa tgggtatcgc
     2461 aggtagcgcg cttgaggaca ttcgcagtgg cgccattctg cacgatattg gcaagatcag
     2521 cattccagac gaaattttgc acaaacccgg gaagttgacc ttggatgaaa gagccgtagt
     2581 tgaaaaacac ccagaaacgg cattcaagct tttatcgcgt attccttttt taagtcaggc
     2641 aatcgaaatt ccatactgcc accatgaaaa atgggatggg acggggtatc cgcgtaagct
     2701 agcaggcgaa gatattccac tttcggcacg catctttgct gttgccgatg tttgggatgc
     2761 cctgagctac gaccgccctt acaacaaagc ctggactcgt gaacagatca ttgaatattt
     2821 caacgagcaa accggcaaac atttcgaccc tgcgatcaca aaaatatttc tctccatggt
     2881 tgaaaaaggc gagatataat ccgtgccgca aaataaaata cgtaaagaaa gatgtaacaa
     2941 atgccagatc agatatatgt agtaggacac atcaaccccg ataccgactc gatcgcctct
     3001 gccatgggat atgcctggtt actgcgcgaa cgtgacgggc ttaacaccat agccgcccgc
     3061 gccggagcct taaacgcaca aacttcatgg gtattgaaaa cactcgggct ggaatctcca
     3121 tttttattga ccgatgcttc accccgcttt gactccgtta tgcaaaggct cgatagtcta
     3181 cgtcccaatg cccagctcgg cagcgcatgg actctcgcca gccgcacggg cgggcttgcc
     3241 ccggttgtca atgaagatgg caccccgtat ggcatcatca gcggcatgag cttgttcaaa
     3301 tatttcagca atatcctcgg tccgcgcccc ggcgatacaa ctgtgcgcga aatgatgtct
     3361 gctgaatgta aagatgctgc agataccagt gtgccaaaat atcaagccaa cgcccacatc
     3421 cgcgattcgc tcaaccgcat tctgcgcgat gagatcgatg attattgggt cgtagatgaa
     3481 agcggaagat atctcggcat tgcccgtcaa cgcaatttgc tgaatccgcc gcgcctcaag
     3541 atcatcctcg tggatcacaa tgaaccgcgc cagtctattg ctgcccttga agaagccgag
     3601 cttctggaaa ttctcgatca ccatcgcctc ggcaacccct atacccatca gcccattcgc
     3661 ttcacagtgg atgtggtcgg gtccacttcg actctcgtca ccgaacagac ggctgaagcg
     3721 ggcttgtctg ccccgcctgc cattgcgggt gcgcttcttt ctggactttt gtcggatacg
     3781 cttatcctaa cctctcccac cacgacagac cgtgaccgcg tcgccgcaga acggttggca
     3841 cgctgggcgt ttgttggagg cagcccccta caaggcgaga ccatcgaaac ctatggaaaa
     3901 gccgttctta gtgcaggtgc tgggctttcc aaccgcaagc cagaagaagt cgtcagcaca
     3961 gatatcaaac cattcgaagg cggtggattc aaatttgcag tggcgcaagc cgaggtgacc
     4021 gacatcatgc agctttcaga acaccttgcg ccactcacca aagccctcga cgacctacgc
     4081 gacaaacgcg gactcgactt tgcgctgtta ctggtcactg atgtggtgcg tggaaccagt
     4141 cgcttgatcg ttacatcaag tgcaccaccc attctcaatg accttcccta cccgccctta
     4201 cccgacggca ctcgcgatgc acccggagtg gtttcgagaa agaaacagtt gttgccagcc
     4261 gtattaggtt tattagaacg ataagcaaat gtccatatag ggcgggtttt gaacccgcct
     4321 tattttgtat aattttgaaa aaccatgccc ttcccatacc tcgacgaaac ccttttactc
     4381 aacgccgaaa cccgtaaaga cttgccgggc tcgttcatcg ccctgcctag tggagtaaca
     4441 cactacgaag aaggcgggga tccaaagggt cagccggtag ttttggtgca tggattttct
     4501 gtcccttact ttattttcga accaactttt gtatttcttt gtcagcaagg ctttcgtgtc
     4561 ttgcgctatg acttgcttgg gcgtggattt tcagatcgtc caaatacaag atacaacgca
     4621 actctgtttg ttcaccaact caaagaactg ttagatgcac taaactacaa aaacatctgt
     4681 cttacagggc tgtctatggg cggagcggtt acctcgtcgt ttattgatgc gtaccctgat
     4741 tatgtttcaa aatacgttct cattgaccca tctggcgcaa gggcagtttc gctctcgcct
     4801 tttctcaaag cggtgagaat gcccgggttt ggcgaactgg ctatcggtct gttcggaagc
     4861 gaaagtatgc ttaaaggcat tgcatcagac ttgttcagtc cagaacttgt gcgtgaattc
     4921 caagaaaaat ataaagtcca aatgcaatat caagggttca agcgtgccat tctttctacg
     4981 atgcgcaatg gcatgttgga ctcttttatt gagacctaca aacgagttgg caaactcaaa
     5041 aaaccgactc ttcttttttg gggaaaacaa gatacaacaa ctccgtttga gcatagtgcc
     5101 gatatatgcg cagtgatgcc gcatgcagaa ttctatgcct ttgacagttg tgggcacatt
     5161 cctcactacg agcgcgcaag cgaggtaaac agaattctgt tagaattttt gaggaagtaa
     5221 ttccctcaaa acaaatacat agtgattggg ggatggatta aaggagcaac cgtgaacaaa
     5281 gaatatattc aaacgttgta ccgctataac cagtggtcga atgcgcggat tctcgacacc
     5341 gccgccaaac tcagccctgc gcaattttta gcaacttcag aacatccaca ccgctattta
     5401 cgcggtgtcc ttacacacac cctctttgcc gaatggattt ggcacaaacg ctggacaggc
     5461 gaatcaccca ctacgcgctt gaagccggaa gacttcccaa ccttcgaagc attgcgtaca
     5521 cgttgggcag aggaagaaag ccaattaatg caattcgttg cgcaagtaac ggacgaaaaa
     5581 ctgaacgcgc cttttaccta tttagccacc gatggcacaa aatatgaaaa tattctgtgg
     5641 gagtcgatgg cacatgtggt caatcatggc atgcaacatc gcagtgaagc cgccgccatt
     5701 ctcaccgaac ttggggtttc tcccggtgat ttagatttca tcctattcac aagaaagaaa
     5761 ctatgacctt caatcgcgcc aagaattttc tctttcggaa tcgtcacaat ctagctgtat
     5821 tcgtcttgct taacctagca tgcgtggctt gtattgtttt tgtagcagcg cgtgtggtgt
     5881 ataccgattc gtcgcgtcac acgggtttga tttggaatct cttcctggca tggattccgt
     5941 tcatccttgc gtattttgct catgctcttt cgtggaaaaa agtttgggta tatctggcaa
     6001 ttccatttgt ggcttttctg tggctattgt tcttccccaa cgctccatac atgttgaccg
     6061 acctgcaaga cctcgctcgc ggcacgggtt atgaagctcc gctttggtac gatgtcatca
     6121 tcgtcgtctg gtgctcgtgg acaggaactt tgctgggcgt catctcgctc tatttgatgc
     6181 aagacattat cgcccgcacc ttccaccgct ggctgggatg ggtcttcgtc tttgcaattt
     6241 cagggctgag cagttttggc atttacatcg gtcgcttcgt gcgcctgaat tcatgggaca
     6301 ttttgcaaaa ccccgccgaa accgccatgg aaattctcgg catcgtcatt gacccaagca
     6361 agcgtctcgc cgcctttacg ttggcatata cctttttctt cttgttcatt tacctgttgt
     6421 tgtattcatt tagtcacatg ctgcaagaac aggtgatccg tgctcaaagc tctccggagc
     6481 aattggcttc tacgcagcca acacaaccag tacattaagc ctgctagtct ttgcaaggaa
     6541 ttggaagccc atctgcttac aatgggtcaa acaccggaga tactatgttt caaaaaatat
     6601 ataacaaact aaaaccattt cgttttcgtc tgactatttt ggggctatta gcagtagcca
     6661 gccttatctc aattgcgtta tttcgctttc gcacccgcat cagcggcacc atggaatatg
     6721 atttcctggt ttggaatctg ttcctcgctt ggttgccttt ggggatgtcg tacatcgctt
     6781 caagttttac gaacaagcgt cccttcgttg tcgccttaat tgtccctgtc gcggctgttt
     6841 tatggctatt attcttcccg aatgctccct acattctgac agacctattc cacttgcgcc
     6901 atccgcgtga gattgtgccc atttggtacg acaccttact catcaactgg ttcgcatgga
     6961 ccggagtctt gttgggtgtc ttttcgcttt tcatgatgca cgacatcgta cggcgtgtgt
     7021 ttgggcgttt catcggttgg ttattcgtgc tcacggtcag cacactttgc ggggtgggca
     7081 tttacatcgg gcgcttcttg cgctggaatt catgggatgt cattctgcat ccttatgaac
     7141 gcatgcgcga gatgctatac tatgtcttgc atccaagcat gcaatcgatt ctttttatta
     7201 gcgtcttctc ggctttgttt gttttcatct acattaccac ttatacattc ggtttattgt
     7261 ttcaagaaca agcccaaccc atttcacagg atatttcatg aatccactca ttctcattcg
     7321 cggcggcggt gacctcgctt caggcgtggc gctccgcttg caccgcgttg gttttcaggt
     7381 tgccattctc gaactggata aacccttggc agttcgtaga acggtctcat tttcagaggc
     7441 ggtttatgaa ggctcacaaa cggttgaagg cgtaaccgca aggcttattt cggcagacca
     7501 gtttcaggtc acgttagagg cgggcgaaat tcctgtgttg atcgacccac atgccaacat
     7561 cctcaacaat caatttctga cgagtccaac ttccacattc gttgtggatg cgcgtttgat
     7621 gaaaacggag ccagagccat tgccagagcg tgtggcgctt catatcgggc tgggacctgg
     7681 gtttacagcc ggagccaatt gccacgctgt gatcgaaacc atgcgcggac atgccctcgg
     7741 cagagtgtat tgggatgggc aaaccgc
//
LOCUS       DAOQNJ010000052         7714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4907_length_7714_cov_9.884711, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000052 DAOQNJ010000000
VERSION     DAOQNJ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4907_length_7714_cov_9.884711"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3991)
                     /locus_tag="PLG52_02690"
     CDS             complement(<1..3991)
                     /locus_tag="PLG52_02690"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNB40378.1"
                     /translation="MDNKKHFWLYDILFILVFLVAGYLRIVGYDWGEGQHQHPDELFL
                     TGVLDNLRAHTCNDPAIPIDACPEENQRWMTVSEYFDSTTSTLNPYNRGFGFFVYGNL
                     PMTVTRVLMEAIGKDDIGSSKFFARQMSALADLLTIFLLYLIVSRLYGQKIGLLASVF
                     SSLAVMQIQQSHFFTSDLFVNLFMFLALLFAVGLVEWQGRTDDQPEELETVIKVEDST
                     ETAEPVKSSTSTQIQILKHPLFYLTVAFGFALGMAMASKINAAAMALLLPFALAIRWL
                     VYDRKQKLSNEYWMQVAILLMVGGFATLVSFRIFQPYAFDGIGLNAQWVKNISEQRAQ
                     ASGAADLPWNLQWIRRTHLYSFENLTIWGLGLPLGILAWIGFLWMGWRILKGEYKHLL
                     LWGWTAFYFAWQSMQFNPTMRYQLPIYPLLAMMAAWVLFEIAGANTPVVNRSNLRKVS
                     ATVLGLTVLTLTAVWAFAFQSIYLRAEPRIAASRWMFENAPAGINVLIQTKEDSFNQP
                     LPIPSGTLITPENPYSVTFSPQREGVFNEVMLGHVANVSSTPTTVDLNIYAYEQPTVS
                     LAHAATVIDPASVIAGNTLPLNLMPEQAFVLAPSQKYVLQLETNGNGIYVSESFSILL
                     QTSEGDFIQTVPISPGLFIAPGNPYQMVFTPLKEGSFNGLKFGYTLDTVKSPSNVSVA
                     VYSASQPDTKLAQASAVLSPTSTDNFRGDSLTLQFDRPIPLITDQQYVLKVETDGSGI
                     FVSGSAIVNETDYDFPLPFRVDGYDPYGGLYSNDDLNLQVYWADDANKIDRFVNILSK
                     ADYIAIPTNHQYAQITRVPERYPLTTFYYRELLGCPEGEDIIKCYRVAEPGMYEGKLG
                     FELEAVFTTYPTLGPIVINDQAAEEAFTFYDHPKVLVFKKTDDFNAENLRAILSTVDV
                     SKAVQLTPKDFASFKSLMLPEDRLANQRAGGTWSDLFSYGWLQNKIPALGLVLWYVFI
                     LVLGLFAYPIARLALPGLRQYAYALGRIVGLALMAWLAWMGGSIGVPYTRLSIGVAFA
                     LIVVAGIGLWMKRKEEFKADWDRHRQFFVLVEIVFLTFFLIDLLIRIGNPDMWHPSKG
                     GERPMDFSYFNAVLKSTSFPPYDPWYAGGYINYYYYGFVLAGTPVKLLGIVPSIAYNF
                     ILPTWFAMVALGAFTVGYGAVASSKADGEADEPSMFNLQLLAGLAGALLTVFLGNLGT
                     IQLIFNAMQRVAAPGGTVPTETGFIQHWVWAFQGIWKMITEKAMLPIGRGDWYWFPSR
                     VIPTGPGNEITEFPLFTFLYSDLHAHMLVMPLTLFIIAWALAFVRSRAQLTRGEWIAS
                     FGIGALMIGA"
     gene            complement(4019..6175)
                     /locus_tag="PLG52_02695"
     CDS             complement(4019..6175)
                     /locus_tag="PLG52_02695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB40379.1"
                     /translation="MESNEHTSPQEELTVLDLYKSVTKDWSSFFKFIRSLGDVRRREE
                     FNQALAQETAQAVMIEQTAEGSQPKRAAVDFPFRAVLALSIAFGAQFLIEPPNRFGML
                     SFALYLAAFGVAIWSYRSDEWRLPALPVAWQRFDPLSFKFIPLVIATVLAVLAFVDFG
                     SGLFSVRNTLIWFLAVAFICYSLWLQVAKTPETDSVDNAPNVRRKKWVQPVLFLIVFG
                     IALFYRLYSLNTVPNEPFSDHAEKILDVYDISQGYPKVFFERNTGREAIQMYWTLLVL
                     TVFNTGFSYLSLKLGTILLGILTLPYVYLLGKEYGSERLALFAMLLFGIAYWPNVISR
                     IGLRFPLYPLFVAPTLYYLIRGFRMQNRNDFILAGIFLGLGLHGYSPFRMVPIFVVVM
                     FGIYFLHAKTKDERRQAIWWLLIVVVISMFVFLPLLRYWLEYPQNFGYRAFTRLSDTE
                     TALPGPAWQIFLSNLYKGLLMFNWDDGEIWVHSVVHRPALDLVSAVFFAVGAIFLIVR
                     YFRAKDWRDLTLLSSIPVLLMPSVLSLAFPGENPSLNRTGGAAIPVILIVAMALDGFL
                     SSFRSEKRRLFIGYGLTFLLLGYSMYLNYDLVFNQFASSYRASSWNTSELGKQLSDFR
                     AKYGQLDTAWIVPFPYWVDTRLPGVWAGVPDRDFAMWQDQLPSSLSYPAPKLFMFWHD
                     DLETEKVLKELYPNGKLTRYTSAVEGKDFYIFLVEP"
     gene            complement(6352..7122)
                     /locus_tag="PLG52_02700"
     CDS             complement(6352..7122)
                     /locus_tag="PLG52_02700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB40380.1"
                     /translation="MNEQRSKDFVFLQVSSLPYFRALLRAVESEFYQDLPLPSPVYDV
                     GCGDGHFASLTFDHKLDVGLDPWRFSMKEAKRYNAYKSLVEADGAQTPFPSNHFASGL
                     SNSVLEHIPHIDAVLKETARVLKPGAPFYFCVPNANHFEELSLRKIFGKPYEMWFRRI
                     QRVQHSDHPDVWEERLERAGFKLERWWHYFSPSALRVLEWGHYFGLPSLVAKALTGNW
                     IISPTKWNLFLTEKYIRKYASTEPIENGTYTFYIARKK"
     gene            complement(7119..>7714)
                     /locus_tag="PLG52_02705"
     CDS             complement(7119..>7714)
                     /locus_tag="PLG52_02705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB40381.1"
                     /translation="FASTHLTQERRPVIGMAARFASEKGVEVLLDAMPAILKKYPKAQ
                     VLFAGTYQYVMGEQAYSDRLMPRIHEYEVNGNWKFLGNLDPVQMAAYYPNLDVITVPS
                     LNSTEAFGLVQIEAMMNGVPSVPSALPGVRQPVKMHGMGVVSEIGNSESLATAILEVL
                     DHKEKYRGDLSAIKAAYNPDTVAQEYEKLFEKLMRKK"
BASE COUNT         2388 a         1818 c         1652 g         1856 t
ORIGIN      
        1 gtgcgccgat catcaaagcg ccaataccaa aggaagcaat ccattcaccg cgagtcaatt
       61 gcgcacgaga gcgcacaaat gccaatgccc atgcaatgat gaacaaggtc aacggcatca
      121 ccagcatatg agcatgtaaa tcactgtata agaaagtgaa cagcggaaat tctgtaatct
      181 cgttaccggg tccggttggg attacgcggc ttgggaacca ataccaatct ccgcgcccga
      241 tgggcaacat cgctttttcg gtgatcattt tccaaatacc ttggaatgcc cacacccaat
      301 gctggataaa acctgtctca gtaggaaccg tcccacctgg ggcagcaaca cgttgcattg
      361 cattgaagat cagttggatt gtccctaaat ttccaaggaa gaccgtgagc aatgcgcctg
      421 ccagcccagc caagagttgc aagttgaaca tagatggttc gtctgcttcc ccatccgctt
      481 tgctgcttgc aacagcgccg tatccaaccg tgaaagcacc caacgccacc atggcaaacc
      541 aagtaggcaa aataaagtta tatgcaatcg acggcacaat gccaagcaat ttcaccggcg
      601 tgcccgccaa cacaaaccca taatagtaat aattgatata gccaccggca taccacgggt
      661 catagggcgg gaagctagtg ctcttcaaaa ccgcattgaa atacgagaaa tccatcgggc
      721 gttcgccgcc tttggatgga tgccacatat ctgggttgcc aatgcgaatg agcaaatcga
      781 tcaggaagaa ggttaagaag acgatctcaa ctaagacaaa gaactgacga tggcggtccc
      841 aatctgcttt gaactcttcc ttgcgcttca tccataagcc gatgcctgcc actacgatca
      901 aagcaaatgc cacgccgatg ctgagtcggg tatagggcac accaatcgag ccgcccatcc
      961 acgccagcca tgccataagt gccaagccaa cgatcctgcc taaagcatag gcatactgcc
     1021 gtaaccccgg caacgccaaa cgcgcaatcg ggtaagcgaa caatcccaac acaaggatga
     1081 atacatacca caataccaat cccaaggcag ggatcttatt ctgcaaccaa ccgtagctaa
     1141 acaagtctga ccatgttccg cccgcacgtt ggtttgctaa acgatcttca ggcagcatca
     1201 ggctcttgaa ggaagcaaag tctttgggag taagttgaac cgctttgctc acatcaactg
     1261 tgcttaagat ggctcgcaga ttctctgcgt tgaaatcatc agtcttcttg aagactaaca
     1321 ccttaggatg gtcataaaaa gtaaatgctt cttcagctgc ttgatcatta atgacgatgg
     1381 gtcccaaggt tggatatgta gtgaaaactg cttcaagttc aaaaccaagc ttgccttcat
     1441 acataccagg ttcagcaact cgataacact taataatatc ttccccttca gggcatccta
     1501 aaagttcacg ataatagaag gtcgttaatg gatagcgttc tggcacacga gtgatctgcg
     1561 catattggtg attggttgga atggcaatgt aatccgcttt ggataagatg tttacaaagc
     1621 gatcaatttt attggcatca tccgcccagt acacttgcaa gttaaggtca tcgttgctgt
     1681 ataacccgcc ataggggtca tatccatcca ctcgaaatgg caatggaaag tcataatctg
     1741 tttcattaac aatcgcagaa ccagaaacaa atattccact cccatccgtc tcaactttca
     1801 gaacatattg ttggtcagta atcaatggga tcggacgatc aaactgaagc gtgagagaat
     1861 ctccgcgaaa gttatcagta gaagtggggc tcaaaactgc cgaagcctga gccagttttg
     1921 tgtctggttg tgaggcggag taaacggcta cacttacatt tgatggtgac tttactgtat
     1981 caagggtgta tccgaacttc aatccattaa aagaaccttc ttttaatggt gtaaagacca
     2041 tctgataagg atttcctgga gcgatgaata agcccggaga gatggggacg gtctgaataa
     2101 aatcgccttc acttgtctgc aacagaatac tgaaagactc tgaaacatag atgccgttgc
     2161 catttgtttc aagttgcaat acatatttct ggctcggcgc caacacaaaa gcctgttcgg
     2221 gcatcaagtt caaaggaaga gtattccccg ctatcacaga tgctggatca ataaccgtgg
     2281 cggcatgtgc caagcttaca gttggctgtt cataggcata aatattaaga tcaaccgttg
     2341 ttggtgtgct cgaaacattt gcaacatgcc ccaacatcac ctcattgaag acgccctctc
     2401 tttgcggaga gaaggtcaca gaatatggat tctctggagt aatcaatgta ccggatggaa
     2461 tcggtaaagg ctgattaaag ctatcttctt tcgtttgaat aagaacattg ataccagcag
     2521 gcgcattttc aaacatccaa cgagatgctg cgatacgcgg ttcagctcgc aaataaatac
     2581 tttggaacgc aaatgcccac acagccgtaa gcgtaagaac tgttaagcca agaacggttg
     2641 cagaaacttt gcgcaaattt gagcgattta ctactggtgt gttcgcccct gcgatctcaa
     2701 aaagaaccca tgctgccatc attgccaata acggatagat cggcaattga taccgcatag
     2761 tagggttgaa ctgcatagat tgccatgcaa agtaaaaagc agtccacccc catagcaaaa
     2821 ggtgtttgta ttcgcctttt agaatacgcc agcccatcca taaaaaacct atccaagcaa
     2881 ggattcccag agggagccca agcccccaaa tggtaaggtt ttcgaatgaa tacaaatgag
     2941 tacggcgaat ccattgcaaa ttccacggca ggtctgctgc gccagatgct tgagcgcgtt
     3001 gctcggagat attcttcacc cattgtgcat tcagccctat accgtcgaaa gcatagggtt
     3061 gaaaaatacg aaaagataca agggtagcga atccgccaac catcaaaaga atcgctacct
     3121 gcatccaata ttcattgcta agcttctgct ttcggtcata aacaagccag cgaatagcta
     3181 acgcaaaagg taagagcaat gccatggcag cagcattgat tttggacgcc attgccattc
     3241 ccagagcaaa accaaacgca accgtcaagt aaaaaagcgg atgtttcaga atttgaatct
     3301 gagtagaagt actgctttta acaggttcag ccgtttcagt gctgtcttca accttaataa
     3361 ccgtttctaa ctcttctggc tgatcatctg ttcgtccctg ccactcgaca agtcccactg
     3421 cgaagagcag ggcaaggaac ataaataggt ttacaaaaag atctgaagta aaaaagtgag
     3481 attgctggat ctgcatcact gctagtgaag aaaatacaga agcaagcaac cctattttct
     3541 gtccatacaa acgcgacaca ataagatata gcaggaaaat cgttaacagg tctgccagag
     3601 cagacatctg ccgcgcaaag aacttcgagg aaccaatatc atctttcccg attgcttcca
     3661 tcaaaacgcg agtcactgtc attggcaaat tgccataaac aaaaaaacca aacccgcgat
     3721 tatacgggtt caacgtagac gtagtgctat cgaagtattc actcacagtc atccagcgtt
     3781 gattttcttc cgggcaagca tcgattggaa tagccgggtc gttacatgtg tgcgcacgca
     3841 gattatccaa cacacccgtc aagaaaagct catcaggatg ttggtgctgc ccttctcccc
     3901 agtcataccc aacaatgcgc aaatatcccg ccactaaaaa gacgagaata aacagaatgt
     3961 catacaacca aaaatgtttt ttattatcca ttgatcactg taaaagggct aaccattatt
     4021 atggttccac caaaaagata tagaaatcct taccttcaac cgctgaagta tagcgggtta
     4081 attttccatt tggatagagt tctttaagaa ccttctcagt ttcaaggtca tcatgccaga
     4141 acatgaacaa tttcggagca gggtaactca agctactagg caattggtcc tgccacatgg
     4201 caaaatcacg gtccggcacg cctgcccaaa ctcctggcaa gcgagtatcg acccaatagg
     4261 gaaatgggac aatccatgct gtatccaatt gcccatattt agcacgaaag tcacttaact
     4321 gtttgccaag ttcagatgta ttccatgatg aagcgcgata ggaactcgca aattgattaa
     4381 agaccaaatc ataatttaga tacatggaat agcccaagag gaggaatgtc agcccgtatc
     4441 ctataaataa acgcctcttt tcggacctga aacttgagag aaagccatca agcgccatcg
     4501 ctacgataag aatcaccggg atagcagcac caccggttcg attcaatgaa gggttttcac
     4561 cggggaatgc cagcgaaagt acagatggca tgagcaggac agggattgaa ctcagtaagg
     4621 ttaagtcacg ccaatcttta gcgcggaagt aacgcacaat taagaagatt gcgcccactg
     4681 cgaaaaacac tgcggaaacc aggtctaatg ccggtcggtg caccacagag tgaacccata
     4741 tttccccgtc atcccagttg aacatcaata agcccttata caggtttgaa aggaaaatct
     4801 gccaagctgg tccgggtaat gctgtttcgg tatcgcttaa acgagtaaat gctcggtaac
     4861 caaaattctg tgggtattca agccaataac gcaataatgg caggaagaca aacattgaaa
     4921 tgacaaccac aatcaatagc caccaaatcg cctgacgtct ttcgtctttt gtctttgcat
     4981 gtagaaaata aataccaaac atgaccacca caaagatggg aaccatacgg aaaggactat
     5041 atccgtgcaa gcctagccca agaaagatac ctgcaagaat aaaatcattt cggttctgca
     5101 ttctaaagcc acggattaga taatatagcg ttggtgcaac aaacaaaggg tatagaggga
     5161 agcgcaaccc aatccgcgaa attacatttg gccagtatgc aattccaaaa agcaacattg
     5221 caaacaaggc aagccgttca cttccatact ccttaccgag caggtaaacg tatggcaacg
     5281 tcagaatacc aaggagaatg gtaccaagct taagactgag gtaggaaaaa cctgtattga
     5341 aaacagtaag cacaagcaat gtccagtaca tttgaatcgc ttcacgccca gtattgcgtt
     5401 caaaaaagac ttttggatat ccttgcgaaa tatcatatac atcgagaatt ttttcagcgt
     5461 gatcactaaa tggttcattt ggaaccgtat taagtgaata taggcgataa aacagtgcaa
     5521 tcccgaaaac aatcaagaac aaaacaggct gcacccactt ttttcgcctg acgtttggtg
     5581 cattatctac tgagtccgtt tcaggcgttt ttgcaacctg aagccataaa ctataacaaa
     5641 tgaacgctac agccaagaac catataaggg tattgcgtac agaaaaaagc ccgcttccaa
     5701 aatcaacaaa ggctaaaacg gctaaaactg ttgcgatcac caaagggata aacttgaaag
     5761 acaatggatc aaatcgttgc catgcaacag gtaaagcagg caaacgccat tcatcagacc
     5821 ggtaagacca aatggcgacc ccaaacgcag caagatacaa tgcaaaggat agcattccga
     5881 aacgattggg aggctcaata aggaattgag caccaaatgc aatgcttaga gcaagaacag
     5941 ctctaaatgg gaagtcaaca gcagcccttt tgggttgcga gccttctgcc gtttgctcaa
     6001 tcatcactgc ttgcgcagtt tcttgcgcaa gagcttggtt aaattcttct ctacgccgta
     6061 catcccctaa cgagcggata aacttaaaaa acgaggacca atcctttgtg accgatttat
     6121 aaaggtccaa aacagtcagt tcttcttgtg gggatgtgtg ctcatttgat tccatatact
     6181 ctctcaacct aaaatgtaaa ttgttgcaga acagggtaat tctatactaa ttttgggcgc
     6241 gtttaagtgc actatattaa gaatttatgg caaaaatagc gaaaagcgcc ctttcaaggc
     6301 gcttttagac agaaaaggct gaaaataaaa tccgtatttt cagcctttta attatttttt
     6361 cctagcgata tagaatgtat atgttccgtt ctcaatcggt tctgtggatg catacttacg
     6421 aatatatttt tcggtcaaaa agagattcca ttttgtaggt gaaatgatcc aattacctgt
     6481 taatgccttt gccactagag agggcaatcc gaagtaatgc ccccattcta agactcgtag
     6541 agcagatggc gaaaaataat gccaccaacg ctccaacttg aagcctgcac gttcgaggcg
     6601 ttcttcccat acatccgggt ggtctgaatg ttggacccgt tgaattctac ggaaccacat
     6661 ttcatatggt ttgccaaata tttttcgaag cgaaagctct tcgaagtgat tggcgttcgg
     6721 cacacaaaaa taaaaagggg cacccggctt aagtacacgc gctgtttctt ttagaactgc
     6781 atcaatgtgc ggaatgtgct ccagaacgga gttgcttaat ccgctggcaa aatgattgga
     6841 agggaacggt gtttgtgcgc catcggcttc gaccaaggat ttataggcat tgtatcgttt
     6901 cgcttctttc atggagaaac gccacgggtc aagcccgaca tccagcttgt ggtcaaatgt
     6961 tagtgaagcg aagtgaccat caccacaacc cacatcataa accggagagg gtaatggcag
     7021 gtcttgataa aactcagact caactgcccg caacagggcg cgaaaatagg gtaatgaact
     7081 cacctgcaaa aaaacaaaat ctttggatcg ttgttcgttc actttttcct cattaacttt
     7141 tcaaacaatt tttcatattc ctgtgcaacc gtatcagggt tatatgcggc tttaatggct
     7201 gaaaggtcac cgcgatattt ttctttgtga tcaaggactt caagaatggc agttgccagg
     7261 ctttctgaat tgccaatctc agagacgaca cccatgccat gcatcttcac aggttgacgt
     7321 acccctggca aagcagaggg cacactgggc acaccgttca tcatggcttc gatctggact
     7381 agaccaaacg cttcagtcga attgagagat ggtacggtaa taacatccaa gttcgggtag
     7441 taagctgcca tctgaacagg gtcgagattg ccaaggaatt tccaattccc atttacttca
     7501 tattcatgaa tgcgtggcat caggcggtct gaataagctt gctcacccat cacatattga
     7561 tatgtccctg caaataacac ttgagcctta ggatatttct tgaggatggc aggcatcgca
     7621 tcaagcaaga cttctacgcc tttttcagaa gcaaaacgcg ctgccatacc aattacgggt
     7681 ctacgctcct gcgtaagatg agtggatgca aaag
//
LOCUS       DAOQNJ010000053         7701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4918_length_7701_cov_9.667669, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000053 DAOQNJ010000000
VERSION     DAOQNJ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4918_length_7701_cov_9.667669"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1139)
                     /locus_tag="PLG52_02710"
     CDS             complement(84..1139)
                     /locus_tag="PLG52_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40382.1"
                     /translation="MLAPVHHVIGLTTIVRERLLPISGNVIARVQQKVTASEVVAETR
                     WAREHALLDVARILGVSPTVADKLIKCKVDDKVAASAEIAVGRGLFPRSVRAPKDGRV
                     VAVGGGQVLMEIGEAKMEVRAGVPGTVVQVVPGRGVVVQAFGGLVQGVWGNGRIDSGV
                     LVNLMEGLDTVLLPNRMDVSLRGSIILGGMIKNVETLQALAELPARGLIASSIQPSLL
                     PKAREMRYPILVTDGIGSLPMNSAAYKLLSTNAKREVTLNTEPYDRYTGARPEVFIAL
                     PISAEPPMPNEVEIFAPGMQVRMRRPPSMGMIGSIVAIKPGLTVLPSGLRAQAAEVKL
                     ENDETVIVPLVNMEVVG"
     gene            complement(1143..2906)
                     /locus_tag="PLG52_02715"
     CDS             complement(1143..2906)
                     /locus_tag="PLG52_02715"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNB40383.1"
                     /translation="MPTSLVTSNSLLAIDVGAANTRAVLFDVIEGEYRFVASGVAPST
                     AEAPYKDVSEGARNAIQALQNVLKRTLLETTARSLILPSQPDGSGVDSLVVTVSAGPA
                     VRTLVVGLLKDVSLESARRLTESTYARILDTLSLSDQRKPEQQIDSILRIRPDMVVIA
                     GGTDGGASRSIQKLLEPIGLASYLMPDEKRPAVLFAGNEKLVDEVKSLVGSLATTLHF
                     SSNVRPSLEVEDIAPAELELAQMFVTIRKKQLKGLDVLDLWAGGHTLPTAYATGRMVR
                     FLSNVYGSNKGLLSVDLGASSSVIAAGFANKSVLKVYPQFGLGENLTGLLNYTTVEDI
                     LRWSTLDIPSSVILDYIHQKALYPNTIAATKEDFSIAQSVAKQALYLAMQAARKDFPK
                     EAASISPTLTPLFDPIIAGGAALNDASRPGQSLLLLLDSVQPVGISTVILDQNNLMPM
                     LGAAASQNNILPVQVLESGAFLSVGTVISPVVKTKYGAPILKAKVAYANGTEANVNLK
                     YGSLEVLPLASGEVANLTIQVSRGVDIGFGPGRGTKSLPVTGGALGVVFDGRGRPLDL
                     PSDSVRRRELIKKWNWTLGGE"
     gene            complement(2912..3619)
                     /locus_tag="PLG52_02720"
     CDS             complement(2912..3619)
                     /locus_tag="PLG52_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40384.1"
                     /translation="MPVLSLDLIAGALSFVFTLMVLSYLIGDNPLFKVAIYLFVGVAS
                     GYVAAVVFWQVLYTKLFLPIGQVLLSGAYTQGVLLIVPFLGVVFILLKAVPGMAGVSR
                     IAMAFLVGAGAAVTIAGALTGTLIPQVNATTNFFDLQAAAARNINMVEVAANGTVLLM
                     GVVTSLAYFHFGANPKADGSTKRFAPINMFAWIGRFFISVTLGAVFAGVYAAALTAFI
                     ERVSSLVFFIKTLLGMP"
     gene            complement(3621..>4853)
                     /locus_tag="PLG52_02725"
     CDS             complement(3621..>4853)
                     /locus_tag="PLG52_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40385.1"
                     /translation="ALFTEMPDWLSNAMEEPASGGMIPEPIKGGDALAPDILPSWVEA
                     MRPSDQGMFNIVNAANDATLESRGALAGLSGVLPLGAGFAPTSKPKAYSIKLNASDEQ
                     VKHAGILEQILAAETSAVSLTSEKSLGSSRVLRWSIAFVLFIVTILTSFLGTQIFSTP
                     QAQPNELKLAVAVSQAIPDSAPVLVAVDYEASRAGEIEAATAPLFDNMLLLKHPRLTF
                     VSSNESGAILAERLMNGPLAFHVQNGVSYLNLGYLSGGQMGIRAFAQNPTSVAPSDVN
                     GQPAWASPVLQNVTALNQFALIILLTDDADAARAWVEQTEDSRGAVPILVVSSAQSAP
                     MIYPYYDSGQVAGIVSGLYGGAIFEQQYNNGRPGLARNYWDAYSIGMLLAMLLLVVGG
                     LVNLGLGVRDHNAMREGK"
     assembly_gap    4854..4953
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4954..6616)
                     /locus_tag="PLG52_02730"
     CDS             complement(<4954..6616)
                     /locus_tag="PLG52_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40386.1"
                     /translation="MAEMITCPVCGTQNPANQAVCQRCQTPLNNALFQPGQSPVHKDT
                     GELEPLLPQWLKDARQSAKEEGETQQQPQQRPKPEAPAKPAASSTDFLAGLQSQAGDD
                     DDEEIPDWLASITGTSAPKQKPNEVVEEPSGTRWVETGNKSDFQQAEPEAEEEVPSWL
                     TGMQQQPNPDEKDELTDWFRSSSSEAPSAQPLSFEQPQQSPTFDSAFTPSASNDTPDW
                     LKQMAANAGEKDPEPASDSSFGSSDWFSQPAESNPAPQADPFAQSSDTPDWLHQMQEN
                     LDSSAQQQPSASPFAASSGDSDLPDWLNQMQAGAVPAEPAQPAEPFNLSSDTPDWLNQ
                     MRSDSTPAESAPSAEPFSFSSDTPDWLSQLPADNAASQQPAADPFNASSSADMPDWLS
                     GLGSVPGGENSPFVESTPAESEPFSSNSDLPSWLAPEQDAAKKTDSKPRWLQSDEEVA
                     SGEPAWLASNDKTLVPESEQAADETPVNDVFSDMPDWLKSAAPASTIFDDAPAFETPK
                     AETPKQQPSSPFESAPAFSEDAFPAQNDALFTEMPDWLSNAMEEPA"
     gene            complement(6616..7230)
                     /locus_tag="PLG52_02735"
     CDS             complement(6616..7230)
                     /locus_tag="PLG52_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40387.1"
                     /translation="MRIITAIIAIVVGILVLLGYFLPQAAIFQVILLDWAIILAGAAT
                     LAGVLNLVTVHSEKIRTRSKDNIYSAILILGLFATFVFSLLLKPQNIYVQTFILNGII
                     VPVEASLMALLAVSLLYAAVRLLRRRADLMGIVFVLTAALLFFGSATLPFGDVPIMST
                     LVRPWVSQILALGGARGILIGVALGTLTTGLRVLFGIDRPYGGK"
     gene            complement(7230..>7701)
                     /locus_tag="PLG52_02740"
     CDS             complement(7230..>7701)
                     /locus_tag="PLG52_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40388.1"
                     /translation="PAVASAGDPTLGLLSQDTLQAGYQAAGVEDAYVHTTGRVTGLSP
                     FSYAAGAMQIPQNENVSTNIVIGHLGAEAGLITDAAERNNIKLIGSSDNLVGQSILFA
                     NTQDPLIGEELFAAGAYLNEDPAHAASLTVQDIFRWLIILILLGGAALKFLGTL"
BASE COUNT         2066 a         1900 c         1884 g         1751 t
ORIGIN      
        1 ttatcatcaa aattagattc ttcaaacgac atggagcact ctcctgaatt aggtggtcat
       61 agttcaacga acgatgggga taattatccc acaacttcca tgtttacgag cggtacgatt
      121 acagtctcgt cattttccaa ttttacttca gcagcttgag cacgcagccc gctcggtaaa
      181 acggtcagac cgggcttaat agccacaata gaaccaatca tccccatgga aggtggacgg
      241 cgcatgcgca cctgcatacc gggagcaaaa atttctacct cattcggcat gggaggctct
      301 gcagaaatcg gcaaggcaat aaaaacttct gggcgagcac ccgtataacg atcatacggc
      361 tcagtattta atgtaacttc acgtttggca tttgtgctca acaatttata ggcggcagaa
      421 ttcatcggca aggagccaat tccatcagtc acaaggatgg ggtaacgcat ctcgcgtgcc
      481 tttggcagca aagatggctg aatgctggaa gcgatcaacc cacgtgcggg gagttccgcc
      541 aaagcctgta acgtttcaac gttcttaatc atcccgccca aaatgatcga gccacgcaag
      601 ctaacatcca tacgatttgg aagtagaacg gtatctaaac cttccatcaa gttgaccagc
      661 acgcccgaat caatgcgacc atttccccat acgccttgaa ctagtccacc aaaggcttgg
      721 acaacaacgc cacgccccgg aacgacttgt acgacagttc ctggaacacc ggctcgcact
      781 tccatcttgg cttcgccgat ttccatcaag acttgcccac cacctactgc aaccacacgt
      841 ccatctttag gtgcacgtac agatcgaggg aaaagtccgc gccctaccgc gatttcagca
      901 gaagcagcca ctttatcatc aactttgcat ttgatcagtt tatctgcaac cgttggggat
      961 acgcctaaga tacgagcaac atctaataga gcatgctcac gtgcccaacg cgtttctgcc
     1021 accacttcac tggcggttac tttttgttga actcgggcaa tgacattccc tgaaatgggc
     1081 aacaaacgtt cgcggacaat ggtcgtaaga ccgattacat gatgtacagg tgctaacatg
     1141 gtctattcgc ctcccaaagt ccagttccac tttttgatta actctcgcct gcgaacagag
     1201 tcagaaggaa ggtcaagggg tcttccgcgc ccgtcaaaga caactccgag tgcaccgccc
     1261 gtaacaggta agctcttggt gcctcgacca ggtccgaaac caatatccac gccacgcgag
     1321 acttgaattg tcaggtttgc cacttcacca cttgccaacg gcaggacttc gagcgagccg
     1381 tacttcaggt ttacattcgc ttccgtgcca tttgcataag ccacttttgc cttcaagatc
     1441 ggcgcaccgt atttggtctt aaccacaggt gaaatgaccg tacctacgct taaaaaagca
     1501 cctgattcaa ggacttgcac tggcaaaata ttgttttgcg atgcagcagc tcccagcatg
     1561 ggcatgaggt tattttgatc gaggataacg gtagaaatgc cgacaggttg taccgagtct
     1621 aagagcaaga gcaggctttg accagggcgt gaagcgtcgt ttagggcagc gccgccagca
     1681 atgatggggt caaacaaggg agtgagagtc gggctgatgg aagcagcttc ctttggaaag
     1741 tcttttcgag ccgcttgcat ggcaagatac aaggcttgtt tcgccacaga ttgagcaatg
     1801 gaaaaatctt cttttgttgc cgcaatagtg tttggataca acgctttttg atggatgtaa
     1861 tcaaggatca cactagatgg aatatccaat gtagaccagc gcaaaatatc ttcaacggtt
     1921 gtgtaattca aaagcccagt gaggttttcg ccaagcccaa attgtgggta taccttcaag
     1981 accgatttat ttgcgaaacc ggcagcaatc acagaagagg atgcgcccaa atctacgctg
     2041 agcaatccct tattggagcc atacacatta gagagaaagc gcaccatgcg cccggtagca
     2101 taagcagttg gaagcgtatg accacccgcc cataagtcca gcacatctaa ccctttgagt
     2161 tgtttcttac gaatggtcac aaacatttgc gccaattcaa gttcggctgg agcaatatct
     2221 tccacttcaa gcgaaggtcg gacgtttgag ctaaagtgta gcgtggttgc gagcgaccct
     2281 accaacgatt tgacttcatc aaccaacttc tcattgcctg cgaacagaac cgccgggcgc
     2341 ttttcatcag gcatcagata gctggcaagc ccaatgggtt cgagcaattt ttggatggag
     2401 cgtgaggcgc ctccatccgt tccaccggcg ataacaacca tgtccggacg gatgcgcaaa
     2461 atgctatcga tctgctgttc aggtttgcgt tggtcgctga ggctaagggt gtcgaggata
     2521 cgtgcatacg tggactcggt cagccggcga gcgctttcaa gcgaaacatc tttgagcaaa
     2581 cccaccacaa gcgttcggac tgctggacct gcggatacag ttactacaag cgaatccacg
     2641 ccggagccat ctggttggct tggcaaaatg agactgcgag ctgtcgtttc gagcaatgtt
     2701 cttttgagca cattttgaag cgcttgaatg gcattgcgag cgccttcaga aacatcttta
     2761 tacggggctt cagccgtgct aggagccaca ccagaagcga caaaacgata ctcgccttcg
     2821 atcacgtcga acaacactgc gcgggtattc gccgcaccga catcgatggc aagaagagag
     2881 ttactagtga ccagtgaagt gggcataata cttagggcat cccaagcaag gttttgataa
     2941 agaagaccaa agacgaaacg cgttcaataa aggcagtcag ggctgcggca taaacgccag
     3001 caaagaccgc acctaaggta acgctgataa agaagcgacc gatccatgca aacatattga
     3061 tcggggcaaa acgttttgtg gagccatctg ctttggggtt cgcaccaaaa tggaaatatg
     3121 ccagtgaggt gacaactccc atcagcagaa cggtgccatt cgcagcgact tccaccatat
     3181 taatgtttcg ggcagcagcg gcttgaaggt cgaagaaatt agttgtggca ttaacctgcg
     3241 gaatcagtgt gccggtcaac gcccctgcga tcgtgaccgc cgcgccagcc cccacaagga
     3301 acgccatggc aattcgagaa acgcctgcca tgcccggcac tgccttcagt aagataaata
     3361 caacacccaa aaacggcaca atgagcaaaa caccttgggt gtatgcaccg cttaacaaga
     3421 cctgtccaat gggaagaaaa agcttggtgt aaagcacttg ccaaaacacc acagcggcaa
     3481 catacccaga agcaacccca acaaacagat aaatggcaac tttgaaaagt ggattatcac
     3541 caatcaaata acttaacacc atgagtgtaa aaacaaagct caaagctcct gcaatcaaat
     3601 cgagagagag tactggcatc ttattttccc tccctcatgg cattatggtc acgcacacca
     3661 agtcccaaat tgaccaaacc tccgaccact aaaagcaaca tggcgagcaa catgccaatg
     3721 ctgtatgcat cccaatagtt tcgcgcaagc ccggggcgac cattgttgta ttgttgttca
     3781 aagatcgcgc cgccgtatag accagagacg attcctgcca cttgcccaga gtcataatat
     3841 gggtaaatca ttggtgccga ttgtgcgctg gacacaacca aaattggtac cgcaccacgt
     3901 gagtcttcgg tttgttcgac ccaagcacgt gcagcatcag catcatcggt cagtaagatg
     3961 atgagagcga attgatttag ggcggttaca ttctgtaaaa caggcgatgc ccatgcgggt
     4021 tgaccattga catcagacgg cgcaacagag gtggggttct gcgcaaatgc acggatgccc
     4081 atctgtccgc cagagagata tcctagattc aggtaggaaa cgccattttg gacgtgaaaa
     4141 gctaacggtc cgttcatgag gcgttcggca aggatcgccc ccgactcatt gcttgaaaca
     4201 aaggtcaggc gcggatgttt caacaatagc atattgtcaa acaaaggtgc cgtagcagct
     4261 tcaatttcac ccgcacgaga agcttcataa tcgacagcga ccaatacagg cgcactatct
     4321 gggatggctt gagaaactgc caccgccaac tttaattcat tgggctgcgc ttgaggtgta
     4381 gagaagattt gtgtaccgag gaaactggtc agaatggtga caatgaataa cacaaaggca
     4441 attgaccagc gcaacacgcg tgaggaacct agagattttt cagaagtgag agaaaccgca
     4501 gatgtttctg ccgcaaggat ctgttccaag atacctgcat gcttaacctg ttcatcactc
     4561 gcattcaatt tgatggaata agcttttggt ttgctggttg gcgcaaagcc tgcccccaaa
     4621 ggcaacacac cagaaagccc cgccaatgcg ccgcgtgatt caagtgtggc atcgtttgcg
     4681 gcattcacaa tgttaaacat tccctggtca gaaggtcgca tcgcttccac ccacgaaggc
     4741 agaatatctg gtgcgagcgc atcgccacct ttgatgggtt cagggatcat cccgcctgaa
     4801 gcgggttctt ccattgcatt cgaaagccaa tcaggcattt cggtaaagag cgcnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagcggg ttcttccatt gcattcgaaa
     4981 gccaatcagg catttcggta aagagcgcat cattttgtgc agggaaagca tcttctgaaa
     5041 atgcaggtgc agattcaaat ggagaagaag gttgctgttt aggggtctct gccttcgggg
     5101 tttcaaaggc aggagcatca tcaaaaattg tcgacgcagg cgcagccgat ttcaaccaat
     5161 ctggcatatc gctaaagaca tcattgaccg gggtttcatc tgcagcttgt tcagattcag
     5221 gaacaagagt cttgtcatta gatgccaacc aagcgggctc ccctgaagca acttcctcgt
     5281 cactttgcaa ccaacgcggt ttggaatccg ttttcttggc tgcatcctgt tcgggagcca
     5341 accaagatgg taagtcacta ttcgacgaga acggctcaga ctcagccgga gttgattcaa
     5401 caaacggaga gttttctccg ccgggcacag atcccaatcc acttagccaa tcgggcatat
     5461 ccgcagaaga tgaagcattg aacgggtctg cagcaggttg ctgagatgcc gcattatctg
     5521 ccggaagttg actgagccaa tcaggcgtat ccgaagagaa gctaaatggc tctgctgatg
     5581 gagctgattc ggcaggtgtt gaatcagacc gcatctgatt caaccaatca ggcgtatctg
     5641 aagagagatt aaacggctca gcgggttgag caggttccgc aggaacagcg cctgcttgca
     5701 tttgattaag ccaatccggt aaatctgaat caccagatga agctgcaaac ggtgaagccg
     5761 aaggttgctg ttgtgccgaa gaatcgagat tctcttgcat ttggtgcaac caatcgggcg
     5821 tatccgaaga ttgggcaaat gggtcggctt gaggagcggg atttgattct gccggttggc
     5881 tgaaccagtc tgaagaccca aatgaagaat cggaagctgg ttccgggtct ttttctccgg
     5941 cattcgcagc catttgtttt agccaatctg gcgtgtcgtt tgatgcagaa ggagtaaaag
     6001 cagaatcaaa tgttgggctc tgttgtggct gttcaaaaga caatggctga gcacttggcg
     6061 cctctgaaga cgatgaacgg aaccaatcgg tcagttcgtc tttttcatca gggttgggct
     6121 gttgctgcat gcctgtcaac cagcttggca cttcttcttc cgcctctggt tcagcttgct
     6181 ggaaatcact tttattgcca gtttccaccc agcgagttcc actaggttct tctactactt
     6241 cattgggctt ttgtttgggt gctgatgtac ctgtgatgct agcaagccaa tcggggatct
     6301 cttcatcgtc gtcgtcccct gcttgagatt gcagacctgc taaaaagtcg gtacttgagg
     6361 cggctggttt ggcaggcgct tctggcttgg gccgttgttg aggctgctgt tgagtctcgc
     6421 cttcttcctt agctgattgt ctcgcatctt tcaaccattg cgggagaagt ggctctagct
     6481 caccagtatc tttgtgaact ggagattgcc ccggttggaa taaagcatta ttaagcggcg
     6541 tttgacagcg ttggcaaact gcttggttgg cggggttttg cgtaccacag acagggcagg
     6601 taatcatttc agccattact tacccccgta tggacggtct atgccaaaca agacacgcag
     6661 acctgttgta agtgtgccaa gtgctacacc gatcaaaata ccgcgcgcac cacccaatgc
     6721 caaaatctga gagacccaag ggcgcaccaa ggtgctcata atgggaacat caccgaaagg
     6781 caatgtggct gaaccaaaga aaagcaaagc tgctgttaga acaaaaacaa tacccattaa
     6841 gtctgctcgg cggcgtaata agcggacagc agcatataac aatgaaactg ccagcaacgc
     6901 cataagagag gcttctaccg gaacaatgat gccattcaaa ataaaggttt gcacataaat
     6961 attttgcggc ttgagcaaca aagaaaatac aaatgtagca aacaaaccca agatcaaaat
     7021 agcactatag atattgtctt ttgaacgagt gcgaatcttt tctgaatgaa ccgtaacgag
     7081 attaagtacc cccgccaagg ttgctgcccc agccagaata attgcccaat cgagcaagat
     7141 gacttgaaag atcgcggctt gcggcaggaa atatcccagc aagacgagga taccgacaac
     7201 aatggcgata atggcagtga taatgcgcat tagagcgtcc ctaagaattt aagtgctgcc
     7261 ccacccaaaa ggatgaggat gatcagccag cggaaaatat cttggacagt gaggctggca
     7321 gcatgggcag ggtcttcatt cagataggcg cctgcagcaa agagttcttc accgatcagt
     7381 gggtcttggg tattggcaaa aagaatggat tgccccacca gattatcaga cgagccaatc
     7441 agtttgatgt tgttgcgttc ggcagcatct gtaataagcc cagcttctgc tcccaaatga
     7501 ccaatgacta tgttcgtaga aacattctcg ttttgaggaa tttgcattgc accagccgca
     7561 taactaaatg gtgataaacc cgtaacacgc ccggtggtgt ggacgtaggc atcttcgacg
     7621 ccggcggctt ggtatccggc ttgcaaggtg tcttgggata acagaccaag tgtcgggtcc
     7681 cctgccgagg cgacagcagg c
//
LOCUS       DAOQNJ010000054         7634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_4988_length_7634_cov_9.883758, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000054 DAOQNJ010000000
VERSION     DAOQNJ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4988_length_7634_cov_9.883758"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLG52_02745"
     CDS             complement(<1..586)
                     /locus_tag="PLG52_02745"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB40389.1"
                     /translation="MKLEVITRIPKEQKYKTPLLFVHGMWHGAWCWDEFFLPFFADAG
                     YHVTALSLRGHAGSEGKIQGSSIMDYVRDVEEVARTLPTPPVLIGHSMGGFIVQKFLE
                     RNTAPVGVLLAPAPPYGVWNGTWQVFKHSPLTLMKILTRFRLHPVVETPESTRWAFFS
                     ADMPQDQLLKYHAKMNDESFRMYLDLLGLNLVRTK"
     gene            complement(659..730)
                     /locus_tag="PLG52_02750"
     tRNA            complement(659..730)
                     /locus_tag="PLG52_02750"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(696..698),aa:Lys,seq:ttt)
     gene            828..1151
                     /gene="trxA"
                     /locus_tag="PLG52_02755"
     CDS             828..1151
                     /gene="trxA"
                     /locus_tag="PLG52_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNB40390.1"
                     /translation="MSSVKYVTEQDFQDEVISSQTPVLVDFTADWCQPCKMIAPIVEQ
                     LAGQWDGKVKVVKLDADQNPNIMMQYGVMGIPTLMLFKGGDVKERITGYLPKDKIEAK
                     VTPHV"
     gene            1205..3724
                     /locus_tag="PLG52_02760"
     CDS             1205..3724
                     /locus_tag="PLG52_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB40391.1"
                     /translation="MSEQQLIQAGISALQSNDKQKAFSIFAQVVQQYPQSERGWFLLG
                     MSTDDTQRRKYCLQKVLSINPNHADAKKQLTLLTPPQPAPPTPTFTSFNRSDFQQQHV
                     PAFVDNEPDATSEFERFSEPLNQPEVQQEPQHQPTPKQTKQIRRKKNNNVILIASISG
                     LCILAFTAMAGFYLLMNSRTTPAVQAANPTQPISATNTTLAMAPVATDTPIPPTPLPT
                     GLPTQPYTPRFEEAACQFDVPPGVNVSCGYAIVPENRTINSGRTIRLAVAIFHSRSSN
                     PKPDPVIFLQGGPGGEAVSLSANAYPILVDPFLSDRDYIAYDQRGTGLSEPALICDEL
                     ERANRQDVYGTIDSGSRELVYQNAFLSCAGLLQTQGVDLSAYNTVENAADLRDIVTLL
                     GYEKVNLYGASYGTRLALVAMRNHPEVIRSAILDSVMPVETNWVTEFPNIANYTLTNL
                     FNACKADPDCNKAYPDIETVFWELASKLDEKPITLSTSAYPMGTVTETIDGSYLLSII
                     MGLSKTTRFIDTAPQTIYRVRDGDYSTLIAAQYSLPYGFEGINTGLYISVLCREHVLD
                     TSPEKLLEAATQLRVKDFVWSPFYGHLDKMFSACKTWGSTGPVLGEKDKVVSDIPSLV
                     ITGTFDGATPPQQGKQVADSLPNSYYFEFPNQGHVPTASDDSGCAMQIALEFLDNPLT
                     EPSRDCMGSLKPVKFLVPYTGDPALELKRENLNGVSLIVPTEWKFVDDGFWWRGSASF
                     DITQVTAFRANISVQDLVDYFSSSINGYRGFDGAPTPSGTREANGHAWTLYYATSNGR
                     PVDVAAVDDKGSSMVIVMFSHPDEHDALYRTVFLPMVDSAK"
     gene            3829..4404
                     /gene="infC"
                     /locus_tag="PLG52_02765"
     CDS             3829..4404
                     /gene="infC"
                     /locus_tag="PLG52_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNB40392.1"
                     /translation="MSANEFRVNEGIRVAEVRLIGPDGGNVGVVSIKEALKIAREAEL
                     DLVEVSPGANPPVCRVMDFGKFIYEKAKKEREAKKSQTKIEVKEIRLRPKTNEAHRGF
                     KVDDARRWLLQGHKVRVTVKFRGREMDYPEIALEDLKEIAADLADVAIIEVPPQMEGR
                     TMLVVMAPNKAGAPKKKEKPEQAETKNEAEA"
     gene            4487..4720
                     /locus_tag="PLG52_02770"
     CDS             4487..4720
                     /locus_tag="PLG52_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HNB40393.1"
                     /translation="MPRKAKPGKKFKLKTHKATSKRFRLTGAGVLVRTKGGKSHLRRR
                     TSDRTKALLSETIVVKGRKFVKRIKRLAPNMEG"
     gene            4818..5159
                     /gene="rplT"
                     /locus_tag="PLG52_02775"
     CDS             4818..5159
                     /gene="rplT"
                     /locus_tag="PLG52_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002507379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNB40394.1"
                     /translation="MRVKGGPQGHLRHKKVLKITKGQRGSKSTLFKRANEAMLKSLWY
                     ATRDRRVRKRDLRKLWIARINAAARLNGTTYSKLVAAMKKANIELNRKMLADIAVRDP
                     QAFAAVVAQTK"
     gene            5222..5782
                     /locus_tag="PLG52_02780"
     CDS             5222..5782
                     /locus_tag="PLG52_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNB40395.1"
                     /translation="MNSILVEKKFALGQSLQIVQGDITIEEVDAIVNAANEQLAHGGG
                     VAWAISKKGGPKIQAESDAWVRKHGQVSHAHPAWTSGGALPAKYVIHAVGPVWINGRD
                     DENQKLQAAVTGSLQVADELKCESISMPAISTGIFGFPKDRAAGIIFETIGDYFDKHE
                     GSTVNVVRIVLFDQSTIDAFLKQWAE"
     gene            5779..6549
                     /locus_tag="PLG52_02785"
     CDS             5779..6549
                     /locus_tag="PLG52_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB40396.1"
                     /translation="MITSNQNSKIKLVRALLGRAKERREAGAFVAEGVRLVEEAVKAN
                     WTCRFALYDETLSERGKSQVESLRSRGVDVEEVSASVMKSISETEAPQGILAVLEQSQ
                     LPIPDSRNFILIPDQIRDPGNLGTLLRSAAASGVQAVLIPPETTDAFAPKVLRSGMGA
                     HFRLPIHAMTWEEIEKTTNGLQVYVADMDGQSCWDVDLSKPIALIVGNEAEGASESAR
                     KLANGKISIPMSGETESLNAGVAGSVLMFEILRQRSNV"
     gene            6589..>7634
                     /locus_tag="PLG52_02790"
     CDS             6589..>7634
                     /locus_tag="PLG52_02790"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HNB40397.1"
                     /translation="MKIRSITYFCNPGYPLKENILREAGDFLSKAKSAYEAAGYEVQT
                     VRVATVSFAKLLGEKHIDGLPKYAEQFTQVMKQNGVVYAALGPALPELPRSYQVIPDA
                     IKASEGVFFSGVMADAQTIHMAQVKACAEIIVKSAPLDPNGFANLHFTALANVGANAP
                     FFPGAYHTSDQPAFGIAVECADMAVDAFANAQSIEEGRQWLVSEMTKHGQAITNIANG
                     LQIEFKGIDFSLAPFPDDAHSLGKAVENMGVPKIGLHGSLAAAAILTDAIERADFPHI
                     GFNGFMQPILEDSILAKRAAEGALTVKDALLYSAVCGTGLDTVPLAGDVTADELTPLL
                     LDLCALALRLNKPLT"
BASE COUNT         2024 a         1914 c         1917 g         1779 t
ORIGIN      
        1 ttttcgtgcg gaccaagttc aaccccagca agtctaaata catacgaaac gactcgtcgt
       61 tcattttggc gtgatacttt agaagttggt cttgtggcat atctgccgag aaaaatgccc
      121 aacgtgtgct ttcaggcgtc tcaacgacag gatgtaaacg aaatcgggtg aggattttca
      181 tcaacgtaag cggactatgt ttgaatacct gccacgtccc attccaaact ccgtacggcg
      241 gagccggagc cagcaaaaca cccaccgggg ctgtatttcg ttcaagaaat ttctggacga
      301 tgaacccgcc catggagtga ccgatcagca caggcggagt tggaagcgtc cgtgcaacct
      361 cttccacatc tcgaacataa tccatgatac tactgccttg aattttcccc tcactaccgg
      421 catgtccacg caggctgagc gccgtgacgt gataccccgc atcggcaaaa aagggcaaaa
      481 agaactcgtc ccaacaccag gctccatgcc acatgccatg gacgaaaagc agcggggtct
      541 tatatttttg ttctttagga attcgagtga tcacttcaag tttcatggca aactctcctc
      601 tcccctttga gtttactgta taaaacaaaa atccctcaca taggtaaggg attttctgtg
      661 agcgcggagg ggatcgaacc ctcaacaaac ggcttaaaag gccgctgctc tgccattgag
      721 ctacgcgccc atctttaaag cggattgcat tctatcacgg cagcatactc acgtcaacgg
      781 aagtaattaa aatagagaac atctcatcca agacctcgga ggcaaccatg agttcagtca
      841 aatatgtaac agaacaagac tttcaagacg aagtcattag ctcgcaaacc cctgtattgg
      901 tagatttcac cgcagattgg tgtcagccat gtaagatgat tgcacccatt gtcgaacagc
      961 tcgccggtca atgggatggc aaggtcaagg tcgtcaaact tgatgcggat caaaacccca
     1021 acatcatgat gcagtacggc gtgatgggca tccccacctt gatgcttttc aaaggcggag
     1081 atgtcaaaga acgcattacg ggctatctac ccaaagacaa gatcgaagcc aaagtcaccc
     1141 cccacgtgta agagtataat ggagccaaca tagtcggctc cattttcttt tatataggga
     1201 aaatatgtca gaacaacaac ttattcaggc gggaatttct gcgctacaaa gcaatgataa
     1261 acaaaaggca ttttcgatct ttgcccaagt tgtacaacag taccctcaat ctgagcgtgg
     1321 ctggtttctg cttggcatga gcacagatga tactcaaaga cggaagtatt gcctgcaaaa
     1381 agttctaagt atcaacccca atcatgcgga tgccaaaaag cagcttacgc ttctcacccc
     1441 gccccagcct gctccaccaa ccccaacatt cacttctttc aaccgctcag acttccaaca
     1501 gcaacacgtc cccgcttttg ttgacaatga gccagacgcc acttcggaat ttgagcgatt
     1561 ctcagaaccg ctgaatcagc ccgaggtcca gcaagagccg cagcaccaac ctactccaaa
     1621 acaaaccaag caaatacgtc gcaagaagaa taacaacgtc atcctgatcg catccattag
     1681 cggactttgc attctggcct ttaccgccat ggctggcttt tatttgttga tgaacagccg
     1741 tacgacacct gctgttcaag cagcaaaccc gacacagccc atttcagcca cgaacacaac
     1801 cttggcaatg gctcctgttg cgacggatac gcccatccca cctacaccac ttcccaccgg
     1861 cttaccaaca cagccataca cacctcgctt tgaagaagca gcttgtcaat tcgatgtgcc
     1921 gcccggggtg aacgtttctt gtggttatgc cattgtgcct gaaaaccgca ctatcaacag
     1981 cgggcggacc atccgcttgg cggttgctat atttcatagc cgaagctcca atcccaaacc
     2041 agacccggtt atctttttac aaggtggtcc gggtggcgag gcggtttcgc tcagcgcaaa
     2101 tgcctaccca atcttagtgg atccattttt gagtgaccga gattacatcg cttatgacca
     2161 acgcggcacc gggctttcgg aaccagcctt gatctgcgat gagcttgaac gagccaatcg
     2221 acaagatgtt tacggtacta tcgatagcgg ttccagagag ttggtgtatc agaacgcctt
     2281 cctctcttgt gccgggcttt tacaaactca aggcgttgac ctcagcgcgt ataacacggt
     2341 tgaaaatgca gccgacttaa gagacattgt cacattactg ggatacgaaa aggtcaattt
     2401 atatggcgct tcatatggga cacgtctcgc attggttgcc atgcgcaatc atcccgaggt
     2461 tattcgcagc gctattttag attcagtcat gccggtagaa accaattggg tgacagaatt
     2521 tcccaacata gccaattaca ctctaacgaa tttatttaat gcctgcaaag cagaccctga
     2581 ttgcaataaa gcctacccag atattgaaac cgtattttgg gagcttgcca gtaaactaga
     2641 cgaaaaaccg ataactttat ctacatctgc gtacccgatg ggaacggtta cagagaccat
     2701 cgatggctcc tatttattat cgatcatcat ggggttatca aaaactaccc gtttcataga
     2761 tactgctcca caaaccatct atcgcgtacg cgatggggat tattcaactt tgatcgccgc
     2821 tcaatattca ttgccgtatg ggtttgaagg cattaatacg ggtctgtata tctcagttct
     2881 gtgtcgcgaa catgttttgg atacatctcc cgaaaaattg ctggaagccg ctactcagtt
     2941 aagagtcaaa gattttgttt ggagcccttt ttatgggcat ttggataaga tgttctctgc
     3001 ctgcaagact tggggctcaa cgggtccggt actcggcgaa aaagataaag tcgtcagcga
     3061 tattcccagt ttggtcatca ccggaacctt cgatggtgca actccgccac aacaaggcaa
     3121 acaggtggca gactcattac ccaatagcta ttattttgaa ttccccaacc aagggcatgt
     3181 gcccaccgca agcgacgaca gcggctgtgc catgcaaatt gctttggaat ttctggataa
     3241 cccgcttaca gagcccagcc gagactgtat gggaagtctg aagccggtca agttcttggt
     3301 gccctacacc ggcgaccctg cccttgaact gaaaagagaa aacctcaacg gagtctcgct
     3361 tattgtccct accgaatgga agtttgtaga cgatggcttc tggtggcgcg gatccgcttc
     3421 cttcgatatc acacaggtca cagcatttcg tgcaaatatc agtgtgcaag acttggtgga
     3481 ctatttctct tccagcatca atggctaccg tggttttgac ggcgctccca ccccatcagg
     3541 gacgcgtgag gcaaatggtc atgcctggac cttatactat gccacatcca atgggcgccc
     3601 ggttgatgta gcagcagtgg atgacaaggg aagttcgatg gtcattgtga tgtttagtca
     3661 cccagatgaa cacgatgcct tgtatcgaac ggtcttccta ccgatggtag attcagcaaa
     3721 ataagccctt tttcgttgac gactcgcctg ccttcatggt attattcgcc cgctaaaaaa
     3781 ccagccatgg gctgggtttg ttttgtctaa cagcaagagg agaagcacat aagcgccaac
     3841 gaatttcgag taaatgaagg catccgcgtc gcggaagtgc gcctgatcgg tccagatggt
     3901 ggcaacgtcg gagttgtgtc catcaaggaa gcgttgaaga tcgcacgcga agcggaattg
     3961 gaccttgtag aggtatcgcc cggtgcgaac ccacccgtct gccgtgtgat ggactttggt
     4021 aagttcatct acgaaaaggc gaagaaggaa cgcgaagcaa agaaatctca gaccaagatc
     4081 gaggtcaaag agatccgctt gcgaccgaag accaacgaag cccatcgcgg cttcaaggtg
     4141 gacgatgccc gccgctggct ccttcaggga cacaaggtgc gtgtgaccgt caagttccgt
     4201 ggacgcgaaa tggattatcc cgaaatcgcg ctcgaagact tgaaagagat cgccgccgat
     4261 ctggctgatg tcgctatcat cgaagtccct ccacaaatgg aaggacgcac catgttggtg
     4321 gtgatggctc cgaacaaggc tggagcgccg aagaagaaag agaaacccga acaggctgaa
     4381 acaaagaacg aagcggaagc ctgaggtgag caagacccaa agatgttgat cgtccgcggg
     4441 cagtgaaatg tccgcggttt attttgtctg aaaggagtac tacgcgatgc cacgcaaagc
     4501 taaacctggc aagaaattca aactcaagac ccacaaggcg acatccaaac gctttcgcct
     4561 gaccggtgct ggcgtgctgg tgcgcaccaa aggtggtaaa agccacttgc gccgccgcac
     4621 ttcagaccgc accaaggctc tcttaagtga gaccattgtg gtgaagggcc gcaagttcgt
     4681 gaagcgcatc aagcgcctcg cacccaacat ggaaggctaa atcttccggt cgtcgtaaat
     4741 tttttatgtg cggctttcgg gtgtgcgcaa ctggtgtaac ccaccgacgc ccgcgagttt
     4801 gcaggaggta ctaaacaatg cgcgttaaag gaggcccgca agggcactta cgtcacaaga
     4861 aagttttgaa gatcacgaag ggacagcgcg gctcgaagag cacactgttc aagcgagcca
     4921 atgaggccat gctcaagagc ctctggtacg ccactcgtga ccgccgcgtt cgcaaacgcg
     4981 atcttcgcaa gttgtggatc gcccgtatca acgcagcggc tcgcctgaac ggcaccacct
     5041 acagcaagct ggtggcagcc atgaagaagg caaacattga gctcaatcgc aagatgctcg
     5101 ccgacatcgc cgtgagggac ccacaagcct tcgccgcagt cgtagcacaa accaagtaat
     5161 ttttatgtag gggcggatcg cgatccgccc ctacaatttt taacgggggt aaaattgatt
     5221 catgaattcc atcctagtag aaaagaaatt tgcattgggt cagtcactac aaatcgtgca
     5281 aggtgacatc accatagaag aagtggacgc cattgtcaat gcggcgaatg aacagctcgc
     5341 ccacggcggc ggagtggctt gggcaatttc aaagaagggt ggaccaaaaa ttcaagcaga
     5401 aagtgatgcc tgggtccgca aacacggaca ggtctcacac gcgcatcctg cctggacctc
     5461 gggcggggcg ctgcctgcga agtatgtgat ccacgctgtg ggtccggtgt ggatcaatgg
     5521 cagagatgac gaaaatcaaa aattacaagc tgctgtgaca ggttctttgc aagtggcaga
     5581 tgaattgaaa tgtgagtcga tctctatgcc ggcaatttct acgggcattt tcgggtttcc
     5641 gaaagaccga gcagctggaa ttattttcga aacaataggg gattattttg ataaacacga
     5701 aggttcaact gtaaatgttg tgcgtatcgt tctatttgac caatccacaa ttgatgcttt
     5761 tctaaaacaa tgggctgaat gatcacatcc aaccagaatt caaaaataaa acttgtgcgc
     5821 gccttgttgg ggcgtgccaa agagcgccgt gaggctgggg catttgtcgc tgaaggggtg
     5881 cggctggtgg aagaggcagt gaaagcgaat tggacatgtc gctttgcctt gtatgatgag
     5941 accttgagcg aaagaggcaa gtctcaggtc gaaagtttaa ggtcgcgcgg cgtggacgtg
     6001 gaagaagtct cagccagtgt gatgaagtcc attagcgaga cggaagcgcc gcagggtatc
     6061 cttgctgtgc tcgaacaatc ccaactccca attcccgatt cccgaaattt catcctcatc
     6121 ccagaccaga tccgcgaccc ggggaatttg ggaacgttgt tacggtctgc agcggcttcg
     6181 ggcgtgcaag ccgttttaat tccgccagaa acgacggatg cttttgcacc aaaagttctg
     6241 cgatctggta tgggagcgca tttcaggctg cccattcatg caatgacttg ggaagagatt
     6301 gagaaaacta caaatggctt gcaagtttac gtcgccgata tggatggaca atcttgctgg
     6361 gatgtggact tatccaagcc gattgctttg atcgttggca acgaagcaga aggggcaagc
     6421 gagtccgcgc ggaagttggc gaatgggaaa attagcattc caatgtccgg cgagactgag
     6481 tctctgaatg cgggagtagc ggggtcggtg ttgatgtttg agatattaag gcaacgttcc
     6541 aacgtttgaa cgtttatagg ttttcaatct tctactgtat gaggaaaaat gaaaatacga
     6601 tccattacct atttctgtaa tcctggttat cccttgaaag aaaatatcct gcgtgaagcg
     6661 ggagattttt tatctaaagc caagtctgcg tatgaggcgg ctgggtatga agtacaaacc
     6721 gtgcgtgtgg cgacggtttc gtttgcgaaa ctgctgggtg aaaaacatat cgatggtctg
     6781 ccgaaatatg ccgagcagtt tacacaggtg atgaaacaaa acggcgtcgt gtatgcagcg
     6841 ctgggacctg ctctgccaga attgccacgc agctatcaag tcattccaga tgcgataaaa
     6901 gcaagtgaag gcgttttctt cagcggcgtg atggcagatg cgcagacgat ccacatggca
     6961 caagtcaagg catgtgcaga gatcatcgtc aagtctgcac cgttagaccc gaatggattt
     7021 gccaacctgc atttcacggc actcgcaaat gtcggtgcga atgcgccatt cttcccgggc
     7081 gcgtatcata cgtctgacca gcctgcgttt ggaattgccg tcgaatgtgc tgatatggca
     7141 gtggatgcct ttgcaaacgc ccaatccatt gaagaagggc gtcaatggct cgtttcagaa
     7201 atgaccaaac atggtcaagc cattacgaac atcgcaaacg gtttacaaat cgaattcaaa
     7261 ggcatcgact tctcgcttgc acccttccct gatgatgcac attcgctggg caaagccgta
     7321 gagaatatgg gcgtgccgaa gatcggttta catggttcgc ttgcagcagc tgcaattctc
     7381 actgatgcca ttgagcgggc agactttccg catattgggt ttaatggttt catgcaaccc
     7441 attctcgaag attctatttt ggcaaagcgc gccgccgaag gtgcgctgac tgtgaaggat
     7501 gctttgcttt attccgccgt atgcggcaca ggtttggata ctgttccgtt ggctggggat
     7561 gtaacagcgg acgaactcac gcctcttctc ttggacctgt gtgctttggc gcttcgattg
     7621 aacaaacccc tcac
//
LOCUS       DAOQNJ010000055         7608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5017_length_7608_cov_7.788296, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000055 DAOQNJ010000000
VERSION     DAOQNJ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5017_length_7608_cov_7.788296"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLG52_02795"
     CDS             complement(<1..488)
                     /locus_tag="PLG52_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40398.1"
                     /translation="MVEINRIDGSFRDPSGFLFKHDQVLYRQVNKRYADEYTLLMSSG
                     LYESLIKSRLLVAHEEVNDIQPAEPKDAFKIIRPQRVNFISYPYEWSFSQLKDAALAT
                     LSIQKRAMKYGMSLKDASAYNIQFHEGKATLIDTLSFEKYTEGTPWVAYKQFCQHFLA
                     PLA"
     gene            complement(475..675)
                     /locus_tag="PLG52_02800"
     CDS             complement(475..675)
                     /locus_tag="PLG52_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40399.1"
                     /translation="MFFSHIALSPVFYLDPGSGSILFQMIVAALLGAGILIRSQWSRI
                     KRWFGVKPEVDENEDNEDNGGN"
     gene            complement(832..1791)
                     /locus_tag="PLG52_02805"
     CDS             complement(832..1791)
                     /locus_tag="PLG52_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNB40400.1"
                     /translation="MLRSFFIYLSKAAWAQKLITGWSFSWRIASRFVAGVTLTDAIKI
                     VRELNAKGINATLDHLGEHTNTPEEAQQAADSIFATLDAIGADPSLRSNISIKLTQIG
                     MGLDESLCAETLERILARAKTAKTFVRVDMEDTPYTDKTINLHYAMREKGYENHGMVI
                     QAYLFRTEADVRRMLQDGVRIRNVKGAYNEPPDKAFPQKADVDANYDLITKILLDASL
                     AQKSKLSDDGRVPPIPAIATHDERRIAFAKSYAEKIGLPKNGMEFQMLYGIRRDLQES
                     LVKEGYLVRVYVPFGTHWYPYFMRRLAERPANVWFFIVNFFKG"
     gene            1917..3407
                     /locus_tag="PLG52_02810"
     CDS             1917..3407
                     /locus_tag="PLG52_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNB40401.1"
                     /translation="MRNRNTNTILRGVSILFLLGAFTLTVVSLISYSRQRTGYPAGMM
                     IAGVPVDGLSPAEASQRLLQVYNSPIEVNYNGAFFHIDPGAVGFTMDVETMLAAADLT
                     RTGGSFWGEFWDYLWNRAPAESEIPLSASISEDRLREFLQNEIASRYDIPPAPAQPVP
                     GGTTFLPGAPGQTLDVNRAVVIITNALRSPADRSVVLTFNQTTASRPTIENLEILLKQ
                     IITTSDFDGLIGYYMMDLQTGQEIHFLINNKQEITVEPDVAFTASSTIKLPILTSYLI
                     NHGTNLDTATTEAITRVFRISDNSATDSILERIDPNNGPIIVSQNMEQLGLTSTYISG
                     MFFLGAPNLIPGKVTPGNSRADIFTDPDPYSQTTPSEMGTLLADIYQCVQNGGGALIA
                     AFPDKVTPDTCKLIIDFLALDKFGALIQGGVPDGTLVPHKHGFVLSRDGVMHDISDVG
                     IVYSPGGNFILSIYTYHPVQNIWDITNPLFVNLTQATYNYFNIAAQ"
     gene            3572..4201
                     /locus_tag="PLG52_02815"
     CDS             3572..4201
                     /locus_tag="PLG52_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40402.1"
                     /translation="MDAIQKTLENDKELKTAQTRLEKNSAENQQALKTLKNIELEAEN
                     LRIKIEQSNSSLYGGTVKNPKELQDLQKEIESLKKHQETLEERQLEAMSQSENTEAEV
                     EKAKAELEVIRSRLGNEHKKLLDEQAELSKQMASFGQEREAALSPIDVSLLTSYEDLR
                     KQKRGVAVTEVEDGACASCGTTINAALQQSARSQKLSYCPSCGRILFAN"
     gene            4211..6751
                     /locus_tag="PLG52_02820"
     CDS             4211..6751
                     /locus_tag="PLG52_02820"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNB40403.1"
                     /translation="MFLLNIIDPLSFIAGFATATVIWFLIGRARPMLAELREGLKEQR
                     EQAQTRKASSVEENHRRLTLRRAQGMHLAAQLFALDEILQEPTVITPPAPVEPGGAAR
                     FEDVVTQTLPYMPAWPEVAAIYQPHTLTLPQALAGDTNLVIIGQPGTGKTVALAHLAS
                     LAANRSEKLGALQSFVPFHFHVADLNLPIQDPKNILNVISDAAAENAPMFDVGKIPTF
                     VQSVFRSGEVLLLVDGYDEITPDEQTLVAEFFKLLLKAHPKTRIVTTGAPEYLDGLIP
                     LGFVPLSLCTWTEKGNQEFVDQWGELWSRTVAMEAWAQTGPEQVDPLLINSWLGSASL
                     NLSPLELTLKIWGAYAGDSLGPNILDTIATHIRRIAPANTPLAALETLAMQVVVSATP
                     VFDPRKAREWVKSFEVAEETPEGETAQSPEGEAQPSEDAKSKKGKKVEKVATPNTGLL
                     GKMTESGLLVSYPNNRMRFIHSVLAGYLAGHALTNYNAEETLLNQPDWSGKYLTMRFI
                     AAHGDASRLVQAMLEFSRLPMHRPLFAAARWLRDAPKNAPWRGKMFGTLAAILQTEGI
                     PLSLRGQAMAAFVYSNDASAATLFRQFATSSSFELIHLAILGLGAVRDTKSLKILEGA
                     LNAPSLAVKRAACMALAAINTNESLEIAAHALLNGEEDVRRAAGEALANDPKEGHAML
                     RDGITINDILLRRAVIYGLGRVREEWATDLLKKVQIEDEQWVVRNAATEVLDMQAQSY
                     AYAPMKPKAPHETPWLIEFAAKKGVGVSPGVPATDILLVALKDENPETRLAAIQFLKY
                     TPQEIVLKQLYEAMYKDDPELREATFNILWELGAAGVKLPHPSEYGLN"
     gene            6753..>7608
                     /locus_tag="PLG52_02825"
     CDS             6753..>7608
                     /locus_tag="PLG52_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB40404.1"
                     /translation="MLITNANIITWESPNRVLSNYAILIEGGKIKEIGTTKSLTAKHP
                     KVKATDVNGQYVMPGGICAHTHFYGAFARGMAIPGPAPKDFPEILKKLWWPLDRSLDA
                     EGVRYSALVSIVDAIKHGTTTLIDHHASPNTIDGSLDVIADVVDKSGLRGVLCYEVTD
                     RDGIEKANAGIQENVRFIKRLASNPHPRLAAAFGLHASLTLSGATLQACAAAAPEGTG
                     FHIHTAEHESDEYDSLQKSGMRVIDRLLKHGILGPKTITAHGVHFDAREMEILKETET
                     WLTHQPRSN"
BASE COUNT         2094 a         1937 c         1679 g         1898 t
ORIGIN      
        1 gccagtggag cgagaaaatg ctggcaaaat tgtttataag caacccaagg tgtaccttca
       61 gtgtactttt caaaggaaag tgtatcaatt aaggttgctt ttccttcatg gaattgaatg
      121 ttataagcac tcgcatcctt aagagacatg ccatatttca tggcacgttt ttgaatagac
      181 aaggtcgcaa gtgcagcatc ttttagttgc gaaaaagacc attcataggg ataagaaata
      241 aagttcactc tctgtggacg aatgatcttg aaagcatctt ttggctctgc tggttgaata
      301 tcatttactt cttcatgagc aacaagcaat ctactcttga ttaagctctc atataaacca
      361 gaactcatca aaagagtata ttcatcagca tatctcttat tgacctgacg atacaaaact
      421 tgatcgtgct taaagagaaa tccgcttggg tcgcggaaag agccgtcaat acgattaatt
      481 tccaccattg tcctcgttgt cttcgttttc atcaacttca ggtttgacgc caaaccaacg
      541 cttaatgcga gaccactgag agcgtatcaa aatacctgcc cccaaaagag cagctacgat
      601 catttggaac aagatgctgc cagatccagg gtcaagataa aatacaggag atagagcaat
      661 atgagagaaa aacatgttta ctttccttat ggtgaaataa tgtcaagact gtttatttta
      721 ttgcagggac atcatggcgt caataaggtt aatgttctgt aaaagcgtaa tcagctttta
      781 aacaaaaaaa gcggtgaaaa tctgtagatt ttcaccgctt tgatgagtct tttagccttt
      841 aaaaaagttg acaataaaga accacacgtt cgctgggcgt tctgccaggc gtctcataaa
      901 gtaggggtac cagtgcgtcc caaacggaac ataaacacga accagatatc cttcttttac
      961 aagagactct tgcaaatctc tgcgaatacc gtacaacatt tggaattcca ttccattttt
     1021 aggcagccct attttttcag catagctttt ggcaaaagca atacgccttt catcatgcgt
     1081 cgcaatcgcc gggataggcg gcacacgccc atcgtcactg agcttcgatt tttgtgccag
     1141 cgaagcatct aataaaatct tcgtgatcag gtcgtagttt gcatcaacat ctgctttttg
     1201 aggaaaggct ttatcaggcg gctcgttata tgcaccttta acattgcgaa tgcgaactcc
     1261 atcttgtaac atacggcgaa cgtccgcttc ggtacggaag agatatgctt gaataaccat
     1321 gccgtgattt tcatatccct tttcacgcat ggcataatgc aagttaatcg ttttatccgt
     1381 atacggcgta tcttccatat caacgcggac aaaggtttta gcggttttag cacgagcgag
     1441 gatacgctcc aaggtttcag cacacaggct ctcgtccagc cccatgccga tttgtgtcag
     1501 cttaatggaa atattgctgc gaaggctagg gtccgctcca atagcatcca atgttgcgaa
     1561 gatactatcc gctgcctgct gcgcctcttc aggggtattc gtatgctccc ctaagtgatc
     1621 caaagtagca ttgatcccct ttgcatttaa ctcacggaca attttaattg cgtccgtaag
     1681 agtaaccccc gcaacaaaac gagatgcgat ccgccaggaa aagctccaac ctgtgatcag
     1741 cttttgtgcc caggcggctt tcgaaaggta gataaaaaat gaacggagca tatccatcct
     1801 ttgaactgtg atttagatta atatcaactt attctagcac aagccctatg tttcggtatg
     1861 ttatactttt tccgtgattt ttgtcatgcc catgacatcg gaggcaatgt ttaaccgtga
     1921 gaaaccgaaa taccaatacc atccttcggg gtgtttcgat cttattttta ctcggggcgt
     1981 tcacactaac cgtcgtttcc cttatctctt atagccgtca aaggacgggc taccccgctg
     2041 gcatgatgat tgccggtgtt ccagtagatg gcttatcccc tgccgaagca tctcaaagac
     2101 ttctccaagt gtacaactcg ccaattgaag taaattacaa cggcgcattt tttcacatcg
     2161 acccaggagc agtcggcttc acaatggatg ttgagaccat gctggcagcc gcagatctga
     2221 caagaacagg cggctctttc tggggcgaat tttgggacta cctctggaac cgagcaccag
     2281 ccgaatctga aatcccgtta agtgcaagca tatccgaaga ccgtctgaga gaatttctac
     2341 agaatgaaat tgcatcgcgg tacgatatcc cccccgcccc cgcacaaccc gtccccggcg
     2401 ggacaacctt cctccccggc gctcccgggc agactcttga tgtcaaccgt gctgttgtga
     2461 tcatcacaaa cgctttgcgc tctcctgcag accgctctgt tgtattgacg ttcaaccaaa
     2521 ccaccgcatc acgcccaacc atcgaaaatc ttgaaatcct gctcaaacaa atcatcacca
     2581 catcagactt tgacggtttg attggttact acatgatgga cttgcagact gggcaggaaa
     2641 ttcacttttt gatcaacaac aaacaagaga tcacagtaga accagacgtc gctttcactg
     2701 catccagtac gatcaagttg cccattctga cttcatacct gatcaatcat ggcacgaatc
     2761 tcgatacagc aactacagaa gccattaccc gtgtgtttcg tatttcagac aactctgcta
     2821 cagattccat ccttgaacgt atcgatccca ataacggtcc gattatcgtc tcccaaaaca
     2881 tggaacaact cgggctgacc agtacctaca tcagcggtat gttcttcctt ggtgcaccaa
     2941 acttgattcc aggaaaggtc acccctggca attctcgcgc agacattttc acagacccag
     3001 acccctattc gcaaacaact ccgtccgaaa tgggcacctt gctagcagac atctaccagt
     3061 gcgtccaaaa tggaggcggg gcgttaatcg cagccttccc agacaaagtc actccagaca
     3121 cgtgcaagct catcatcgac ttcctcgcgt tggataaatt tggtgccctc attcagggcg
     3181 gcgttccaga tggcacgctc gtccctcaca agcacggatt tgtgctctcg cgtgatggcg
     3241 tcatgcacga tatcagtgac gttggcatcg tgtactctcc gggagggaat ttcattctct
     3301 cgatttatac ctatcatccg gtgcaaaaca tttgggatat tacaaacccg ttgttcgtca
     3361 atctcacaca agcgacgtac aattacttca acatcgcggc gcaataaccc aaacaccccg
     3421 ttctttcatg aacggggtgt ttttatatac tttccatttc aaaaaagggc attttaagac
     3481 cgtgccgggt ataatttgat ttatgagtgc atcgctggga cttttccgcc ttcaacaagt
     3541 agaccgtcag attgaccgcg ccaaagcacg tttggatgcc attcaaaaga ccttagaaaa
     3601 cgacaaagaa ctaaaaacgg cgcaaacccg tttagaaaaa aacagtgctg aaaaccagca
     3661 agccctgaaa accttgaaaa atatcgaact tgaagccgag aaccttagaa ttaagatcga
     3721 gcaatcgaat tcaagccttt atggtggcac agtaaaaaac ccgaaagaac ttcaagacct
     3781 gcaaaaagaa attgagtctc ttaaaaagca ccaagagact ttagaagaac gtcaactaga
     3841 agccatgtcg caatcggaga ataccgaggc tgaagtagaa aaagcaaaag ccgaattgga
     3901 agtgattcgc tcgcggcttg gcaatgaaca caaaaaattg ctggatgaac aagccgaact
     3961 gagtaaacaa atggcaagtt ttggtcagga gcgtgaagca gccctttctc ctattgatgt
     4021 ttctttactt acctcttacg aagaccttcg caaacaaaaa cgcggagttg cagtaactga
     4081 agtagaagat ggtgcttgtg catcttgtgg cacgaccatc aacgcagcac ttcaacaaag
     4141 tgctcgttct caaaaacttt catattgccc ttcttgtggg cggatcctct tcgcaaacta
     4201 ggcgagtgac atgttcttat tgaatatcat tgaccctctt tcattcatag caggttttgc
     4261 taccgccacc gtcatttggt tcttaattgg gcgtgctcgc cccatgctcg ctgaattgcg
     4321 tgaagggtta aaagaacaac gcgagcaagc ccaaacccgc aaagcaagtt cggttgaaga
     4381 aaatcaccgc cgccttactt tacgccgtgc acaggggatg catcttgccg cacaactctt
     4441 tgcattggat gaaatcttac aagaacccac cgtcatcaca ccgcctgcac cagtagaacc
     4501 cggcggcgca gcccgctttg aagatgtagt cacccaaacc ctgccctaca tgccagcctg
     4561 gcccgaagtg gcagcaatct accaacccca tacacttact ctgccacaag ccttggctgg
     4621 ggatacaaac ctcgtcatta tcggtcagcc cggcaccggt aaaactgtag ctctcgctca
     4681 tttagcaagc cttgccgcaa accgcagtga aaaattaggt gcactgcaaa gcttcgttcc
     4741 gtttcacttt catgtggcag acttaaatct gccaattcaa gaccccaaaa atatactgaa
     4801 tgtcattagc gacgctgccg ccgaaaatgc acctatgttc gatgtcggca aaattccgac
     4861 atttgttcaa tccgtctttc gaagcggaga agtgctttta ctcgttgatg gctacgacga
     4921 gattacaccc gatgaacaaa ccttggtggc agagttcttc aaactattgc tcaaagctca
     4981 tccaaagacg cgcatcgtca caactggtgc gccagaatat ttggatggtt taattccgct
     5041 tggctttgtg ccgctaagtc tgtgtacatg gacagaaaaa ggcaatcagg aatttgtgga
     5101 tcaatggggc gagttatggt ctcgcaccgt tgcaatggaa gcttgggcac aaacgggacc
     5161 tgagcaagtg gatccgcttc ttatcaactc ctggcttggt tctgcgtctc taaatttatc
     5221 tccccttgag ttgactctca aaatttgggg cgcatatgca ggcgatagtt taggtcctaa
     5281 cattctcgat accatcgcca cccatattcg acgcattgct ccagccaaca caccgcttgc
     5341 cgcgcttgag acgcttgcca tgcaagttgt cgtcagtgcc acgcccgtat ttgacccgcg
     5401 caaggcacgt gaatgggtga agtcgtttga agtggcagaa gaaactcctg aaggcgaaac
     5461 agctcaaagt ccagaaggtg aggcgcagcc atcagaagat gccaagtcta aaaaaggcaa
     5521 gaaggtcgaa aaagttgcta cgcccaatac aggtctgctc gggaagatga ccgaaagcgg
     5581 attgttagtg tcatatccca ataaccgtat gcggtttatt cattcggtat tggctggata
     5641 tcttgcgggt catgcattga ccaattacaa cgccgaagaa acattgttga atcaacccga
     5701 ttggtctgga aaatatctca ccatgcggtt tattgccgca catggtgatg ccagccgatt
     5761 ggtacaagcc atgctcgaat tttcacgcct acccatgcac cgaccgctat ttgcggcggc
     5821 tcgctggcta cgtgatgcgc ctaaaaatgc tccatggcgc ggaaaaatgt ttggaaccct
     5881 cgccgcaatc ttgcagacag agggcatccc cctcagcctg cgcggacaag ccatggcagc
     5941 ctttgtgtat agcaatgatg ccagcgcagc aaccctattc cgccagtttg ccacatcaag
     6001 ttcattcgaa ctgattcatc ttgccattct tgggcttggc gccgtacgcg atacgaaatc
     6061 cttgaagatt cttgaaggcg cattaaatgc tccgagcctt gctgtaaaac gggcagcgtg
     6121 tatggcattg gcagccatca acaccaacga gtcgcttgaa attgccgcac atgctttgtt
     6181 gaatggcgaa gaagatgtgc gccgtgccgc aggtgaagcc cttgccaatg atcccaaaga
     6241 aggtcacgcc atgttgcgcg atggtattac catcaacgat attctcctgc gccgtgctgt
     6301 gatttatggt cttgggcgtg tccgcgagga atgggcgact gacctgctta agaaagttca
     6361 aattgaagac gaacaatggg ttgttcgtaa tgccgctaca gaagtgttgg atatgcaggc
     6421 acaaagctat gcctatgcac ccatgaagcc taaagctccg catgaaaccc catggcttat
     6481 tgagtttgcc gccaagaaag gcgtgggcgt ttcacccggt gtccctgcca cagatatttt
     6541 gttggttgcc ctgaaagatg aaaaccctga aactcgcttg gctgccattc aattcttaaa
     6601 gtacacacca caagagattg tgctcaaaca attgtacgaa gccatgtata aagatgaccc
     6661 cgaattacgg gaagcaacct tcaacatcct ttgggaactt ggcgcagcgg gcgtcaaatt
     6721 accgcaccct agcgaatatg gattgaacta atatgctgat cacaaacgca aacatcatca
     6781 catgggaatc tcccaatcgc gtcctttcaa attacgccat tcttatcgag ggcggaaaga
     6841 tcaaggaaat cggcacaacc aaatcgctta cagccaaaca tcccaaagta aaagccactg
     6901 acgtaaacgg tcagtatgtg atgccgggcg gcatttgcgc acacactcac ttttatggtg
     6961 cttttgcgcg cggcatggca attccgggtc cggcgccaaa ggattttcct gaaatcctca
     7021 aaaaattatg gtggccactt gaccgttctc ttgatgcaga gggcgtgcga tactctgcct
     7081 tggtttctat cgtggatgca atcaagcatg gcaccactac tctcattgat caccatgcat
     7141 ctcccaacac tattgacggc tcgctagacg tcattgctga tgtggtagat aaaagcggct
     7201 tacgtggtgt gctttgctac gaagtaacag accgtgatgg aatcgaaaaa gccaatgcag
     7261 ggattcaaga gaatgtgcgc ttcatcaaac gtctcgcctc aaatccacac ccgcggttgg
     7321 cagctgcttt tgggctgcat gccagcctga cactttcggg cgcgaccttg caagcctgtg
     7381 ccgccgcagc ccccgaaggg acaggcttcc acatccatac agccgagcat gagtccgatg
     7441 aatacgatag cttgcaaaaa tctggcatgc gtgtgattga ccgcttgcta aaacatggca
     7501 tcttaggtcc aaagaccatc acggcacatg gtgtccactt tgatgcacgt gaaatggaaa
     7561 tcctcaaaga aaccgaaacc tggcttaccc atcagccccg ttctaata
//
LOCUS       DAOQNJ010000056         7564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5067_length_7564_cov_9.666800, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000056 DAOQNJ010000000
VERSION     DAOQNJ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5067_length_7564_cov_9.666800"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2341)
                     /gene="uvrA"
                     /locus_tag="PLG52_02830"
     CDS             complement(<1..2341)
                     /gene="uvrA"
                     /locus_tag="PLG52_02830"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNB40405.1"
                     /translation="MSSNKIRVVGARVHNLKNITVEIPRDKFVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFIGQMDKPDVDYIDGLSPAVSIDQKSTSHNPRSTVGT
                     VTEIYDYMRLLFARAGIPHCPVCGREVSKQSAQEIVDKIANLAEGTRILILAPLVRGR
                     KGTYQAVFEEIRKAGFTRARVDGNVYSLDDEIELDRYKQHTIEAVVDRLVISHEGSKE
                     DKKAALTRLTDSIETALKFGEGYITVNLVDKKEDVAYSEHLACPEHGSTVPEIEPRTF
                     SFNTPQGACPDCQGLGSKLEIDPVRIIPDDSVSLNDGAIASMEWGPREEGGYYWSTLV
                     TAAKHYKIDLDAPFRDLTKEQQHVILYGTGDKQLPMKYTNSSGFEVKFSRAFEGVITN
                     LERRYKETNSEYIREKISEYMSDRPCPSCGGKRLNPAALAVTVDNVNIVEANSWPVLQ
                     TLEWVKKISGKSSPLTNKQKTIAERVIREIHERLNFLVNVGLDYLTLNRSAVTLSGGE
                     AQRIRLATQVGSRLVGVLYVLDEPSIGLHPRDNTRLLETLKGLRDLGNTVLVVEHDDE
                     TILESDWVIDLGPAAGEHGGQIIAEGTPKQILAHPKSLTGLYLSGRKQVEIPKKRREG
                     NGKLLKIVNATANNLKGIDVNIPLGKLVCITGVSGSGKSTLMSDILYNELAAKLMGAR
                     TQSGEHKTIEGIEHLDKIINIDQSPIGRTPRSNPGTYTGLFDEIRKLFAELPESKVRG
                     YKPGRFSFNVHGGRCEACQGQGELRIEMQFLPDVYVPCDV"
     gene            2545..5055
                     /locus_tag="PLG52_02835"
     CDS             2545..5055
                     /locus_tag="PLG52_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNB40406.1"
                     /translation="MSTKNKVSTEQSSISPELASTIPSNLPVLRTKLIGREAEQKTVL
                     DLLNQTDIRLVTLVGFGGAGKTTLALHTSHALFEKFPGGLFFIDLTSIYEPALILPTI
                     AATLGLQEEPSREIADTLRDFLSNRSILFVLDNFEQLISGANIIASFLDANPHIRLLV
                     TSREALHLRGEHTLSLAPLESADAMQVFTQYAQTLNPHFRLTEDNTPAITELCNKLDG
                     LPLAIELAAMRTRMFTPQALLARLTSDLETGSPLLATLTSGPRDMPERQRTLRNMVAW
                     SYNLLTDKEKHMLQAAALFRSGFGIGSLAQVTAVPENEAEHILASLVDKNLIQPFHGD
                     EVRFHLLESIREFALEQALKQEDVNTLTEKFVGYYLALARDAEQGFRSPEQVQWLDLL
                     DDELANIQTALRFALNDGYAGSFFQSGYEMLIHLQRYWLLRAHYAVGVSWLEKAKAII
                     DRQVAISSGVISNDLLYQKSKIYGFLGYAYWIMGRYAIAKDLHEISLTAARTLNREDL
                     LWESLNNSAVNLEYMGEFEAAMTYYAQALEIIKRTGDRWQEMRLLINLGNSYVGIEEY
                     ETGRTYLEDALLLTMELKDDYYQAACLQGLGYLELRLRRHKEAEENFLKSLSLFERMD
                     VPFIYTWALVSLARVLGETSKHKETAQVVLKCVEMLETQKDRNLYRTMFETLVAFCIS
                     LNKNDMAARFIGFIEQNYAGESEVILPADKAIFDAKTQQVGSMMDQREYVLEKETGAK
                     GSASLLFQLASGICDEILNDTHSKPVLLELLTERELSVLRLLSQGRTNEEISKELVVV
                     LKTVEKHVANILRKLGAKNRTEAAIWYLENTSNLRQND"
     gene            complement(5101..6003)
                     /locus_tag="PLG52_02840"
     CDS             complement(5101..6003)
                     /locus_tag="PLG52_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40407.1"
                     /translation="MKRQTLVVILNLALTFSLFGSVSIAHAQTTCTDATGATSPCPPS
                     GGDSGSNEEDDNGSNQPGGGNPNPPTDITATPSPTPSPEPTSTPLPLQAQPTLDPTKN
                     APVFEVTPTPTAEPVITSSGNDGATEGENDWEDVCTFDLCLGLTAACYWDGGTPEIVE
                     DGSGNTGVHCTIPDDDDGAAAPFPWGPVGIVGIVVLLMVMLLPAVQKFKNKPDTPQGR
                     SWDTFGDIKGDNVAPTSTPQTREHILLAKQQDGNTSGASKPKPKPKPAPQPTPPPAQP
                     KPPSSGTPGDFNSDGTVDGNDFIP"
     gene            6202..6765
                     /locus_tag="PLG52_02845"
     CDS             6202..6765
                     /locus_tag="PLG52_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB40408.1"
                     /translation="MEYHEEDVLMRASQGDKDAFGELYERYAERIFNYVYYRTGNQHD
                     AEDLTARVFQRAMNHIRNYTDRGVPFSAWLYRIAHNLVANWHRDRSRKQEIPIDDLPI
                     LPTKGDHPERNLVRSQEQDTLLKMIRKLPPERQNLLILKFVENMSNAEIGKIMGRSEG
                     AVKSLYHRTLLALRDQLEDQNLNLEGE"
     gene            6768..>7564
                     /locus_tag="PLG52_02850"
     CDS             6768..>7564
                     /locus_tag="PLG52_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNB40409.1"
                     /translation="MLRIVTDGAVDLPTEWYKEYDIHRIPINVHFGEEKTFIQDVELD
                     QDGFYKLVDGKTLPHPKTSQPSPHQFAEFYKKIAQPGDTILSIHITSKLSGTYASSVA
                     AAEELKGTYTVVPVDTLCGTMGTAFMCRSARQMERAGKSVDEIKTFIEGVRSKTHVIL
                     TLETLEYAKRSGRVGTLSAALASVLNVKPIAMLKDGIVDMVDRVRTRKGAIDRVLDLA
                     KENVGDSPVHIGVVHARDEASAKSLLEEAQKRFNVKESFLTDLSISLV"
BASE COUNT         1891 a         1741 c         1927 g         2005 t
ORIGIN      
        1 acacatcgca aggcacatac acatctggca aaaattgcat ttcgatgcgt aactcacctt
       61 gtccctgaca ggcttcacag cgcccgccat gaacattaaa ggagaaacgc cctggtttgt
      121 acccgcgcac tttagactca ggaagttctg caaacaactt acgaatttca tcaaacaagc
      181 cagtgtatgt accgggattc gaacgtggcg tgcgtccgat aggggattgg tcgatgttaa
      241 taatcttatc gagatgttca attccttcaa tcgttttatg ctcgccagat tgtgtccgcg
      301 cacccatgag ttttgccgca agttcgttat acaggatgtc gctcatcagt gtggacttgc
      361 ccgaacctga gacgcctgta atacaaacta atttcccaag agggatattg acatcaatgc
      421 ccttcaaatt atttgctgtt gcattaacaa ttttgagcaa cttgccattg ccttcgcgcc
      481 tctttttcgg gatctcgacc tgtttgcgcc ccgagagata gagtccagtc agtgactttg
      541 ggtgcgccaa aatttgcttc ggtgtgcctt ctgcaataat ttgtccaccg tgttcgcccg
      601 cagcaggacc taagtcgatg acccaatctg attcaagaat ggtctcatca tcatgttcaa
      661 cgacaagaac cgtatttccc agatccctta accctttaag cgtttcaagc agacgggtgt
      721 tatcacgcgg gtgcaaacca atggatggtt catctaaaac gtagagaacg cctacgagac
      781 gagaaccaac ctgagttgca aggcggatgc gttgcgcttc tccgccagag agagtcacag
      841 cagagcggtt caaggtcaaa taatccaagc cgacgttaac gaggaagttc aagcgctcat
      901 gaatttcacg aatgacacgt tctgcaatgg ttttttgctt attagtaagt ggcgagcttt
      961 tgccagaaat ctttttgacc cattcaagcg tttgtagcac gggccatgaa ttcgcttcca
     1021 caatgtttac attatctaca gtcacggcaa gagcagcggg gttgagacgt ttgcctccgc
     1081 acgatgggca tggacgatcg gacatgtatt ctgagatttt ttcgcggatg tattccgaat
     1141 tggtctcttt gtaacggcgt tcgaggttgg tgataacccc ttcaaacgca cggctgaact
     1201 tgacttcaaa tccgctcgag ttggtgtatt tcattggaag ttgtttgtcg cctgtgccgt
     1261 agagaatgac atgttgttgt tcttttgtca gatctctaaa tggagcatca agatcaatct
     1321 tgtaatgctt agcggcggtg acaagtgtgc tccagtagta accgccttcc tcacgcggac
     1381 cccactccat actggcaatt gctccatcgt tcagtgacac actatcatca gggatgatgc
     1441 gaacgggatc aatttccaac ttggaaccaa gtccttggca gtcagggcag gcgccttgag
     1501 gcgtgttaaa ggaaaaggta cgcggctcaa tttcgggcac ggttgagccg tgttctgggc
     1561 aggcgagatg ctccgagtag gcgacatctt cttttttatc gacaaggttg acggtgatat
     1621 atccttcacc aaatttgaga gctgtttcaa tcgagtctgt tagacgggtg agcgcagctt
     1681 tcttatcctc ttttgaacct tcatgcgaaa tgacgagacg atctactacg gcttcaatgg
     1741 tgtgctgttt gtaacggtca agttcgatct catcatccag cgagtagacg tttccatcga
     1801 cgcgggcacg ggtaaaacct gctttacgga tttcttcaaa gacggcttgg tatgtacctt
     1861 tacgtccgcg cactagtgga gcaaggatga ggatgcgtgt accttcagcc agattagcga
     1921 tcttatccac aatttcttgt gccgactgtt tgctcacttc gcgtccgcag acggggcagt
     1981 gcggaatgcc cgcgcgcgca aacaagagac gcatgtaatc gtatatctcg gtcactgtgc
     2041 caacggtaga acgcgggttg tggctggtgg atttctggtc gatggaaacg gcaggggaga
     2101 gcccatcgat ataatcgaca tcgggcttgt ccatctgacc gatgaactgg cgtgcatatg
     2161 ccgaaagcga ctcgacataa cgcctctgac cttcggcgaa gatggtatca aacgccagcg
     2221 acgatttgcc cgaacccgaa agcccagtga tgacgacgaa cttatcgcgt ggaatttcca
     2281 cggtgatatt tttcaaatta tggacgcgtg cgcccacgac gcggatttta ttggatgaca
     2341 tgagactcct gtttgggaga aggtattata gcacaggtgt tctggtaatc gtagtccctg
     2401 gcaaaaagaa aaaatgatta taccaatcag ggtgaacacg ccttctcatt tcatgtaaat
     2461 tgtggtgtct tacaaaatca tactttttgg ggagtaagtt gtgccataaa aaagagacca
     2521 acccgttata attttttact gaccatgtct acaaaaaaca aagtatcgac agagcaatca
     2581 agcatctcgc ctgagcttgc ttctacgatc cctagtaact tgcctgtctt gcgcacaaag
     2641 ttgatcggac gtgaagcaga gcagaagact gtcttggatc tcctaaacca aaccgatata
     2701 cgtcttgtga cccttgttgg gtttggcggt gcgggcaaaa ccacgttggc attgcacacc
     2761 tcacatgctc tgtttgagaa atttcccggc gggctgttct ttattgacct gacttccatt
     2821 tacgaacccg ctttgatttt gccgaccatt gccgcaacac tgggtttgca agaagagccc
     2881 agccgcgaga tcgccgacac cctgcgcgat ttcctgtcca atcgttctat cctttttgtg
     2941 ttggataatt ttgaacagct cattagcggc gcaaatatca ttgccagttt tctcgatgcc
     3001 aacccgcaca ttcgtttgct cgtcaccagc cgtgaggcgt tgcacttgcg cggtgagcac
     3061 accctgtcgc ttgctccgct cgaatcggca gatgccatgc aggtcttcac tcaatatgca
     3121 caaaccctca acccgcattt tcgattaacc gaagataaca cccctgccat taccgaactt
     3181 tgtaacaaac tggatgggct tccgcttgcc atcgaacttg ccgccatgcg cacgcggatg
     3241 ttcaccccgc aagccctgct cgcccgcctc acatcggatc tcgaaactgg ttcgccactt
     3301 ttagccactc ttacatccgg tccgcgtgac atgcctgaac gtcaacgcac cctgcggaac
     3361 atggttgcct ggagttacaa cctgctcaca gacaaagaga agcacatgct gcaagcggcg
     3421 gcgctcttcc gctcgggctt tggcataggc tcactggcgc aggtaacagc cgtccctgag
     3481 aatgaagcgg agcacatcct cgcctcattg gtggataaaa atcttatcca gcctttccat
     3541 ggagatgagg ttcgttttca tctcttggaa agtatccgcg agtttgcgct ggagcaggct
     3601 ttgaagcaag aagacgtgaa taccctcaca gaaaagtttg ttgggtatta ccttgcattg
     3661 gcgcgagatg ccgagcaggg ttttcgtagc cctgaacagg tgcaatggct ggatctgctt
     3721 gatgacgaac ttgccaatat ccaaaccgca ttgaggtttg cacttaatga tggatatgca
     3781 ggttcgttct ttcagagtgg gtatgaaatg ttgatacact tgcagcggta ttggctgttg
     3841 cgtgctcatt atgcagtcgg tgtatcctgg ctggaaaagg cgaaagccat catcgatagg
     3901 caggttgcca tttcatcggg ggttatctca aatgatttgc tttatcagaa atcgaagatc
     3961 tatggctttt tggggtatgc atattggatc atggggcgct acgcgattgc aaaagattta
     4021 catgaaatat cactgacggc tgcgcgtacc ttgaatagag aagatctatt atgggaaagt
     4081 ctcaataact ctgctgtcaa tctggaatat atgggtgaat ttgaagcggc gatgacatat
     4141 tatgcacagg cgcttgagat cattaagcgg acgggtgatc gttggcaaga aatgcgttta
     4201 ctcattaatc ttggaaattc atacgttggt attgaagaat atgaaactgg gcgtacctat
     4261 cttgaggatg ccctgctttt gacaatggag ttgaaagatg actattatca agccgcttgt
     4321 ttgcaagggc ttggttatct tgaacttcgg ttgcggcgac acaaggaggc ggaagaaaat
     4381 tttttgaaat cgctatcctt gttcgaacgc atggatgtgc cgtttattta cacatgggca
     4441 ttggtcagtt tggcgcgtgt tctgggcgaa accagcaaac acaaggaaac cgcgcaggtc
     4501 gttttaaaat gtgtagagat gctggaaaca caaaaagacc ggaacctata ccgaaccatg
     4561 ttcgaaaccc tggttgcttt ttgtatttca ctgaataaaa atgacatggc tgcccgcttt
     4621 attgggttta ttgagcagaa ttatgcgggg gaaagcgagg ttatcctacc ggcagacaag
     4681 gcgatctttg atgcaaagac gcaacaggtt ggcagcatga tggatcaacg tgagtatgtg
     4741 ttggagaaag aaaccggcgc caaaggcagc gcttcacttt tattccaact agcctctggt
     4801 atctgtgatg aaatcttaaa cgatacacac tcaaaacctg ttttactgga attactcact
     4861 gaacgcgagt tgagtgtact gcgtttgctt tcgcagggca ggacaaacga agagatttca
     4921 aaggaactgg tcgttgtact taagaccgtt gagaaacatg tggcaaatat tctcagaaag
     4981 ttgggcgcca agaaccgcac cgaagcggca atttggtatc tggaaaacac aagcaaccta
     5041 cgtcaaaatg actgacgcgg gttgcttgtg tgaaattaat aggtttgaga ttcgcaatta
     5101 ttaagggatg aaatcatttc cgtctacggt accatcagaa ttaaaatctc cgggagttcc
     5161 actgctgggt ggcttgggtt gagcaggggg cggagttggt tgtggcgcgg gtttgggctt
     5221 gggtttgggt ttggaagcgc ctgatgtgtt gccatcttgt tgctttgcca aaagaatatg
     5281 ctcgcgagtt tgtggcgttg aggtgggtgc aacattgtca cccttaatat ctccgaaagt
     5341 atcccaagac cttccctgcg gagtatctgg tttatttttg aacttttgaa ctgcgggcag
     5401 gagcatgacc ataagcaaaa caacaatccc aacaatacca accggtcccc aagggaaggg
     5461 agccgcagcg ccatcgtcgt catctggaat ggtgcaatga acgccagtgt tgcctgaccc
     5521 gtcttctacg atctcgggtg tgccgccatc ccaatagcag gctgcggtca agcccaggca
     5581 aaggtcgaag gtgcagacat cctcccagtc attttctccc tcggttgcgc catcattgcc
     5641 agaggaagtg attacaggct cggcggtggg tgtgggagtc acttcaaaga caggtgcatt
     5701 tttagtgggg tctagtgttg gttgtgcttg aagtggtagg ggcgtactcg tcggctcggg
     5761 agatggagtg gggctgggtg tagccgtaat atctgtgggt gggttggggt ttccaccacc
     5821 tggctggttg ctgccattgt cgtcctcttc attagagccg ctatcaccgc cagagggcgg
     5881 gcaggggctg gtggctccgg tggcatcggt acaggtggtt tgggcatggg caatggatac
     5941 agacccgaac aaagagaagg tcagcgccag attcaaaatt acaactaacg tttgtctttt
     6001 cataaaaagc tcctttgcat tggttttaac aataagtgtt tgcattatcc ccaaagcggt
     6061 tctgcttgtc ggtagggtta accctactct tccccctgtt ttttgatagg aaattggtca
     6121 tgttatactg agtgcaactt ctcaaatccg gcggtgtttt attattcaag ttccgccggt
     6181 aaggtttctc acttgtcgga tatggaatat cacgaagaag atgtgttaat gcgcgcctca
     6241 caaggcgaca aggatgcatt tggcgagttg tatgaacgct atgcagagcg tatcttcaac
     6301 tatgtgtact atcgcacggg gaaccaacac gatgcagaag atttaacagc acgtgtattt
     6361 cagcgggcga tgaatcacat tcggaattac accgatcggg gtgtgccatt ttcggcgtgg
     6421 ttatatcgta ttgcgcacaa tctggttgcc aattggcacc gtgaccgcag ccgaaaacag
     6481 gaaatcccca tcgatgactt gcctatcctg ccaacgaagg gtgaccaccc tgagaggaat
     6541 ttagtccgct cgcaggagca ggatacacta ctaaaaatga tccgcaaatt accgcccgaa
     6601 aggcagaatt tgcttatttt gaaatttgtt gaaaatatgt ccaacgctga gatcggcaag
     6661 attatgggta gaagcgaagg agccgtgaag agcctgtacc accgcactct tctggcgctt
     6721 cgagaccaac tggaagatca aaatcttaat cttgaaggag agtaagcatg ttaagaattg
     6781 tgacagacgg cgctgtggac cttccaacgg agtggtacaa ggaatacgat attcatcgca
     6841 ttcccattaa tgtacatttt ggtgaagaga aaaccttcat tcaagatgtt gaattggatc
     6901 aagatgggtt ttataagtta gtagatggaa aaacccttcc tcatccaaaa acttcgcagc
     6961 cttcgcctca ccagtttgcg gagttctata agaagattgc tcaacctggc gatacgatcc
     7021 tttccattca tatcaccagc aagctctcag ggacatatgc ttccagtgtg gctgctgctg
     7081 aggaattaaa aggaacctac acagttgtac cagtcgatac cttgtgtgga acgatgggca
     7141 ctgcctttat gtgccgttct gcccgccaaa tggagcgtgc tggcaagagt gtagatgaaa
     7201 taaagacttt tattgaaggt gtgcgtagta agacccacgt cattctcacg cttgagacgc
     7261 ttgaatatgc caaacgtagt gggcgtgtag gcaccctttc agcagcactt gcttcggtat
     7321 tgaatgtcaa accgattgct atgttgaaag atggcattgt ggatatggtc gatcgtgtgc
     7381 gcactcgtaa aggtgccatt gatcgcgtgc tcgaccttgc aaaagaaaat gtgggcgata
     7441 gcccggttca tatcggtgtt gtccatgctc gtgatgaggc ttctgccaag tctctgttag
     7501 aggaagcgca gaaacgcttc aacgtcaaag aaagcttttt gaccgacctt tccatctccc
     7561 ttgt
//
LOCUS       DAOQNJ010000057         7475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5167_length_7475_cov_9.248113, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000057 DAOQNJ010000000
VERSION     DAOQNJ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5167_length_7475_cov_9.248113"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLG52_02855"
     CDS             complement(<1..536)
                     /locus_tag="PLG52_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40410.1"
                     /translation="MRVKQTLFAILFVAILAACGGEETPDIAAPTPVPSHTPAPSATP
                     IPTLSTPLALLILPADMDQETSDLYQKTVYDLALASGFRFQVRNGITPEDLADPTLKV
                     VIALPPDPGVASYAPTAPNVQFLAVNIPNLTAGGNVSVLAPNTQVELPAFLAGYTLAM
                     MTYEYHIGMLYPEGDPNS"
     gene            complement(618..1001)
                     /locus_tag="PLG52_02860"
     CDS             complement(618..1001)
                     /locus_tag="PLG52_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNB40411.1"
                     /translation="MTLYIQPYPFRRMVRRTVAQPQSRALSVNVREEDDAYVLYALVP
                     GLKAEDLNIQVIENVVSIEGEYKDEETSFLLSELPRGAFSRSLRMPSEIDADKVEARI
                     AEGVLTLTLPKAESAKPKKIKVNVH"
     gene            1117..1821
                     /locus_tag="PLG52_02865"
     CDS             1117..1821
                     /locus_tag="PLG52_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40412.1"
                     /translation="MLKKIFPFLALAVIVAAAGIYLYFTYFRGRSSARTAQVFDWFQD
                     QTSRPELMMTSGLKCGDAPFIFPTNGLIGFIWDDSFRPGHRHSGLDIFSGTEVGVTPI
                     VAAYPGYLTREADWISTVIIRVPEDPLKPNRQIWVYYTHMADPNGNSFVSSAFPQGTK
                     EVFVEAGTFLGYMGNYSGDPANPVGIHLHISVVKDDGFGNYTNELDINNTYDPSPYFG
                     MPLNANENTDVIPVCN"
     gene            1828..2415
                     /locus_tag="PLG52_02870"
     CDS             1828..2415
                     /locus_tag="PLG52_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB40413.1"
                     /translation="MDPLKGKVVLITGAGKGIGRKLAETLAAQGAIVAANDISPINVD
                     EVVNGILAKGGQVKAYLEDVAKKVGAQMIVKSVEDEFGSLDILINHAAVEPHTPLLNM
                     DEWDWHRVLDVNLTGAFLMSQTAGRVMKEKGSGVIINLVAGAGERSKEAGAFLASKAG
                     LDELSRQLARELNPHGVKVVSASTLEEAIKALEEV"
     gene            2412..2882
                     /locus_tag="PLG52_02875"
     CDS             2412..2882
                     /locus_tag="PLG52_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB40414.1"
                     /translation="MKELFEYREKFLARLREVTVEFCDACQAFKDPFTAVDGEWTVHQ
                     IAFHVRGIEREVYGTRVRRTLQEDNPEFKNFDPETWMADSYNRDENLETILDEFSKRM
                     NEVCDLLGDMPQEGWSRLSRHEALGKGLTLQLWAERGLAHIEEHLKALKKAQTL"
     gene            2928..3584
                     /locus_tag="PLG52_02880"
     CDS             2928..3584
                     /locus_tag="PLG52_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB40415.1"
                     /translation="MKKQRIILVDDHEVVRLGLKSLLERHPHFDVVGEAGSAREALEQ
                     TALLKPDVVVMDIRLPGTSGIEACEQIVAQFPNTKVIMLTSYAEDEMLFSAIRAGASG
                     YILKQIGSEDLIKALESVGRGEALLDPAVTQRVFQEVRRAVKEEEASAFAHLSQQEKH
                     VLLLVSEGKTNREIAKNLFLGEGTVRNYVSSILSKLNVNNRAEAAAYAVEHNLRDYIS
                     "
     gene            3669..4235
                     /locus_tag="PLG52_02885"
     CDS             3669..4235
                     /locus_tag="PLG52_02885"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNB40416.1"
                     /translation="MFPFVKLTDDVVMIRPYEFGDEHELHAAIQESLPELNPWMSWAT
                     ERYTLNVARDFVAITRAEWSRGTLYSFAILNASTNEFLGGCGLSHFHPVYKFCNLGYW
                     VRTSKHGNGYAGCATKLVAKFAFERANAIRAEIVIAKDNHASKRVAEKVGAHYEGILL
                     NRMTVRENIYDAHMFSLLPSDFGLVAQL"
     gene            4354..5211
                     /locus_tag="PLG52_02890"
     CDS             4354..5211
                     /locus_tag="PLG52_02890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNB40417.1"
                     /translation="MNESKSIHERKHVWRDEFLLVIGLCVFVGIVYALDNALHPVFTP
                     TTLLLTGVFLAIVPAFIWMIFFYLQDSAEPEPKGYVIGVFVLGGLLAASIGIPVVENL
                     FRVSHWIYTNTVATILGGILVVGFTQEFLKYAAVRYSIYHSDEYDEPTDGVIYATAAG
                     LGYATMLNINFVISNGGVDLGTGIIRMAVVALAHAAFSGITGYFLGRAKFESEQIWWM
                     PLGITLAATFNGLFNWLQGIVTRPTITLSGSTNNPWLGLALAAVVALITTGLVLTLVR
                     RNIKSLQAE"
     gene            5222..5797
                     /locus_tag="PLG52_02895"
     CDS             5222..5797
                     /locus_tag="PLG52_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40418.1"
                     /translation="MANKQGSLGDRFAEITVVLVTLIALSAGWFYKSSIENASVPFEA
                     DGISAAAPKGWLQIGAEGDELLRTVDINSSGFGAVYTIRQIAITSEATSSEVASLVTL
                     DHAQNLTAFKVLNQNEIVDRNGRSAYMIDYVYVESNPDVTHAAIPSVVRGTDFVYLSS
                     DRAIVVSFQADEKNYERDLGRFYLFLESLKF"
     gene            5813..7465
                     /locus_tag="PLG52_02900"
     CDS             5813..7465
                     /locus_tag="PLG52_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNB40419.1"
                     /translation="MKPNFNKYLWMFFTILAIVSLACKAASPSIEPVTTSDEPVSSDG
                     SLSSNARANLISATVQIFGLFDDNGELVPGYVGSGTILSPSGLILTNAHVASPASQGE
                     PESEPDALGIAMIVSEDKPAVPTYIAKVLAVDGFLDLAVIQITSSINGSPIDPGSLNL
                     PYVQLGNSDTVHVGDTINIFGFPAIGGNTITFTKGIVSGFSSEDQLGDRAWIKTDATI
                     SGGNSGGLAADANGHIIGVPTIAAASRDTETSDCRQVQDTNGDGTVDQNDSCVPIGGF
                     LNGIRPVNLAQPLIDAALAGRQYTSTYTQEGVVSNPGNGNESASGFTWLDTSSSNDQG
                     CDWSDQTVNSYGDSVLCIAAGFDYAGMTDGELVVERWFLNDEQVGEYSYTWEWGESGL
                     FGTFLPNDGNPMPAGTYRLEMYAGDAMTPMGNSSTVTVASGGGNSGQQSQPSSSGDTI
                     SVYGQVYDAATNNPISGAYVFVLTSGTTYDEWESSDYADKYIETYLQTDSNGKYKVTD
                     ISRNKEFTLVFAAQGYYDASADNMIANSSDPDSFELNVGLNK"
BASE COUNT         1845 a         1785 c         1929 g         1916 t
ORIGIN      
        1 tgtgagttcg ggtcaccttc tgggtagagc ataccaatat gatattcata ggtcatcatc
       61 gccaaagtat agcctgccag aaaagcaggt aactctactt gggtgttcgg ggcaagaacg
      121 ctaacatttc cgcccgcagt gagattgggg atatttacag caaggaattg cacattcggt
      181 gcagtcggcg cataacttgc cacaccaggg tcgggaggca aagcaatgac tactttcagg
      241 gttgggtccg cgaggtcttc gggagtgatt ccattgcgaa cctggaagcg gaagccagaa
      301 gccaatgcca ggtcatacac cgttttttgg tataagtccg atgtctcctg gtccatatcg
      361 gcggggagga tgagcaatgc caacggcgtg gataaggttg ggatgggcgt cgcagatggc
      421 gccggagtat gagacgggac gggcgtcggg gctgcgatat ccggcgtctc ttccccacca
      481 caagcggcta ggattgcgac gaaaagaatt gcaaaaagag tttgttttac gcgcactgaa
      541 tttctccgtt acaaaatgtc cggaacatta taacgggaaa aaaacaggtc acgcattgtg
      601 cgtgacctgc ggcttgatta gtgaacgttg actttgatct tcttcggttt ggctgattct
      661 gccttgggca gtgtcaacgt taggacgcct tctgcgattc tggcttcgac cttgtctgca
      721 tcaatttcag acggcattcg caagctgcgg ctgaaggctc cgcgtggaag ctcgctcaga
      781 aggaaggatg tttcttcatc cttatattcg ccttcgatgc tgacaacatt ttcgatcacc
      841 tgaatgttca ggtcttcagc tttcaaaccg gggacgagcg cataaaggac atatgcatca
      901 tcttcttcgc ggacgttaac actaagagcg cgggactgag gttgagcgac ggtacggcga
      961 accatacggc ggaacggata aggttggata tagagggtca tgtttatgtc tccttttgtt
     1021 tatgtcaatg acgcaatatt aaatctgcaa tgtaagaaaa atgctaatac ttggtagatt
     1081 ttttataaga aagttcaggg cggtataatc ttttggatgt tgaaaaagat atttccgttt
     1141 ctggctcttg ccgttattgt cgctgcggca gggatttact tgtacttcac ctattttcgc
     1201 ggcaggtctt ctgcgcgaac cgctcaggtt tttgactggt tccaagacca gacttctcgc
     1261 cctgagctga tgatgaccag cggcttgaaa tgtggcgatg ctccattcat cttccccacc
     1321 aatggcttga tcggcttcat ttgggacgat tcattccgcc ccggtcaccg ccacagcggg
     1381 ttagacattt tcagtgggac ggaggttggc gtaacaccca ttgtggctgc ctacccaggt
     1441 tatctcactc gcgaagcaga ttggatttct acggtaatta tccgtgtgcc agaagacccg
     1501 ttgaagccca atcgccagat atgggtgtat tacacccata tggcagaccc aaatggaaat
     1561 tcttttgtct cttctgcctt tccacaaggc accaaagaag tctttgttga agcagggaca
     1621 ttccttgggt atatgggtaa ttattccggt gacccggcaa acccagtggg aatccatttg
     1681 cacatttcgg tggttaaaga tgacggcttc gggaattaca caaacgagtt ggacattaac
     1741 aacacctacg acccaagccc ctattttggg atgccgttga atgcaaatga aaacacagat
     1801 gtaataccag tgtgtaatta agaaaggatg gatccattga aaggcaaagt ggttttaatt
     1861 accggagcag gcaagggaat tggtcgcaaa ttggcagaaa ctttggcggc tcaaggtgca
     1921 attgtggcag ccaatgacat ctccccaatt aatgtggatg aggtggtgaa cggaatcctc
     1981 gcaaagggag ggcaggtgaa ggcttatttg gaagatgttg ccaaaaaggt cggtgcgcaa
     2041 atgatcgtca aaagcgtgga agatgaattt gggtcgcttg atatattgat caaccatgca
     2101 gcggtggaac cgcatacccc tttgctcaat atggatgaat gggattggca ccgcgtgttg
     2161 gatgtaaatt tgacgggcgc ttttctaatg tctcaaacgg cggggcgggt gatgaaagaa
     2221 aaaggctccg gcgtgattat caacttggtg gcaggagcag gcgaaaggtc gaaagaggcg
     2281 ggagcgtttc ttgccagcaa agccggcttg gatgaattgt cccgccagtt ggcacgagaa
     2341 ttaaatccgc atggtgtgaa ggtggtctct gcttcgactt tggaagaagc tatcaaagca
     2401 ttggaggaag tatgaaagaa ctgttcgaat atcgcgaaaa atttttagca cgcctgcgcg
     2461 aagtgacggt tgagttttgt gatgcatgcc aggcattcaa agacccattt acagccgttg
     2521 atggcgaatg gacggtgcat cagatcgctt ttcatgtgcg tggcattgaa cgtgaggtgt
     2581 atggcacacg cgtccgccgc accttgcaag aagacaatcc cgagttcaaa aattttgacc
     2641 ctgaaacatg gatggcggat tcttataatc gtgacgagaa ccttgaaact atcttagatg
     2701 aattttcgaa acgcatgaat gaagtctgcg atctattggg tgacatgcca caagaaggtt
     2761 ggtcacgttt gagccgacac gaagccttgg gcaagggtct aactttgcag ttgtgggcag
     2821 aacgcgggtt ggcacacatt gaagaacatt tgaaagcttt gaaaaaagca caaaccctat
     2881 aatttttgcc cgtttttcca atttcctgcg gtaaaatagt gcctattatg aagaaacaaa
     2941 gaatcatctt ggttgacgat catgaagttg tgcggcttgg actgaagtcg cttttagaga
     3001 gacaccccca ttttgatgtt gtgggtgaag ctgggtcggc gcgtgaagcg ttggaacaga
     3061 cggctctcct caaacctgat gtggtggtga tggacattcg cctgccggga acctctggca
     3121 ttgaagcttg cgaacagatt gttgcgcaat ttcccaacac aaaagtcatc atgctcactt
     3181 cgtatgcaga agatgaaatg ctcttttctg ccattcgtgc gggtgcttcg ggctacattc
     3241 tcaaacaaat tggcagcgaa gatttgatca aagccctcga atcagtggga cgtggtgaag
     3301 cgctactaga ccctgctgtg acccagcgcg tcttccaaga agtgcgccgc gccgtgaagg
     3361 aagaagaagc ttcagccttt gcacacttaa gccagcaaga aaaacacgtg cttctactcg
     3421 tctctgaagg caagaccaat cgcgagatcg ccaagaacct cttcctcggt gaaggaacgg
     3481 tccgcaatta tgtttcgagc atactctcga agttaaatgt gaacaaccgc gctgaagcag
     3541 cagcctacgc ggtggaacac aatttgagag attacatttc ctaattcgtt ttctcagcca
     3601 ctatgaaaga gacactttcg caccccgcgt gaagaagtgt ctcttttttt attcccgcct
     3661 aaaaaactat gttccctttt gtaaaattga ccgatgatgt tgtgatgata cgcccatatg
     3721 agttcgggga tgaacacgaa ctgcatgctg cgattcaaga atcgctaccg gagttaaatc
     3781 catggatgag ttgggcaacc gaacgataca cgctcaatgt cgcgcgcgat tttgtcgcca
     3841 tcacgcgtgc agaatggtca cgcggcacct tatattcttt tgccattctg aatgcaagca
     3901 caaacgaatt tcttggcggt tgtggcttaa gccattttca cccggtctat aagttttgta
     3961 acctcggcta ttgggtgcgg acatccaagc atggcaatgg gtacgccggt tgtgccacaa
     4021 aacttgtagc aaagtttgcc ttcgaacgcg ccaatgcgat tcgtgcagag atcgtaattg
     4081 caaaggataa ccacgccagc aaacgtgtag cagaaaaagt gggagcgcat tacgaaggga
     4141 ttttgctgaa tcgcatgacc gtccgcgaga atatttatga cgcgcatatg ttctcgcttc
     4201 tcccctcaga ctttgggctg gttgcccaac tgtaagcatt ttctccgctc agtctgtccc
     4261 ccatttgtaa cccagctcta ttgacgtaca cccatctttt tactatgatg gggttgttat
     4321 ttgatggatc acttaataag gagagagaat tccatgaatg aatccaaatc aattcatgaa
     4381 cggaagcatg tatggcgtga cgagttcctg ctcgttatcg gcttatgtgt ttttgtgggt
     4441 atcgtctacg ccctagataa tgccctacac cccgtgttta cccccaccac cttgctgctt
     4501 acaggtgtct ttcttgccat tgtccctgca tttatttgga tgattttctt ctatctgcag
     4561 gatagcgccg aacctgaacc gaaaggctat gtgatcggcg tcttcgtatt gggcgggctt
     4621 ttagcggcgt ccatcggtat tcctgttgta gaaaacctct tccgcgtttc tcattggatc
     4681 tataccaata cagttgccac cattctaggc ggaattcttg tagtgggctt cacacaagag
     4741 tttctaaagt atgctgccgt tcggtatagc atctatcatt ccgacgaata cgatgaaccg
     4801 actgacggtg taatctatgc cacagctgcc gggttgggct atgcgaccat gctcaacatc
     4861 aactttgtca tctccaatgg cggcgtggac ttgggcacag gcatcattcg tatggcagtt
     4921 gttgcccttg cgcatgcggc attctcgggc attacgggct acttcctcgg gcgtgccaag
     4981 ttcgaatctg aacaaatttg gtggatgcct ctcggcatca ctctggctgc cactttcaac
     5041 ggcttattca attggttgca aggtatcgtc accagaccga ccatcacttt gagcggctcg
     5101 accaataatc cttggctggg cttggcgctt gcggcagttg ttgcgttaat caccaccggg
     5161 cttgtgctca ccttggtgcg ccgcaacatt aaatcgctgc aggctgagta ggagaacaga
     5221 catggcaaac aaacaaggtt ctctcggtga ccgctttgcg gagatcaccg ttgttcttgt
     5281 aaccctgatc gcgctctcag cgggatggtt ctataaatcg agcattgaaa atgcctcggt
     5341 cccgtttgag gcagatggaa tcagtgccgc agcgcccaaa ggctggttgc aaatcggtgc
     5401 agagggcgat gaactgcttc gtaccgtaga catcaactca agcggtttcg gtgccgtcta
     5461 cacgattcgt cagatcgcga ttacatccga agcgacaagt tccgaagtgg cgagtcttgt
     5521 cactttggat cacgctcaga atttaactgc gttcaaagtg ttgaaccaaa acgaaattgt
     5581 agatcgaaac gggcgcagtg cgtacatgat cgattatgtg tatgtcgaat caaaccccga
     5641 tgtgactcat gctgccattc cgagcgtggt acgcggcaca gattttgtgt acctgagtag
     5701 tgaccgcgcg atcgtggttt cttttcaggc agatgagaaa aattatgaac gcgatctcgg
     5761 aaggttctat ctcttccttg aatcgctcaa gttttagtgt agcgaggcga gcatgaaacc
     5821 aaacttcaat aaatatcttt ggatgttctt cactattctt gccatcgtca gcctggcatg
     5881 taaggcagcg tctccttcca tagaacctgt tacaacgtcg gatgagccgg tttcttccga
     5941 tggttcttta tcctccaacg cacgtgccaa cttgatctcg gctaccgttc agatctttgg
     6001 tctatttgat gacaacggtg aattggtgcc ggggtatgta ggctcgggca cgatcctctc
     6061 cccctctggc ttgattctca ccaacgcgca tgttgccagc ccggcttcgc aaggcgaacc
     6121 tgaaagcgaa ccggatgcac ttggcattgc aatgatcgtt tctgaagata agcccgccgt
     6181 gccaacctac atcgcgaaag tcctagctgt ggatggcttc ctggacttgg ctgtgatcca
     6241 gatcacatcg agtatcaacg gctctcccat cgacccgggc agtctcaacc tgccttatgt
     6301 gcagttgggc aactctgaca ccgttcacgt gggcgatacg atcaatatct ttggcttccc
     6361 cgccattggc ggaaatacca tcacgtttac aaaaggcatt gtctccggct tctcgtctga
     6421 agaccaactc ggcgaccgag cgtggatcaa aaccgacgcc accatctcgg gtgggaactc
     6481 aggcgggctc gccgcagatg cgaacggaca tatcattggc gtgcccacta ttgccgccgc
     6541 cagccgtgat acagaaactt ctgattgccg tcaagtgcaa gatacaaacg gcgatggcac
     6601 tgtagaccaa aacgatagct gtgtgcctat cggtggcttc ttgaacggca ttcgcccggt
     6661 caaccttgcc cagccattga ttgacgcggc gcttgcaggc agacaataca ctagcaccta
     6721 tactcaagaa ggtgtagtga gtaaccccgg taacggaaat gagtcagcta gcggctttac
     6781 ttggctagat acatccagca gcaacgacca aggttgtgac tggtctgacc agaccgtcaa
     6841 ctcgtatggc gattcggtct tgtgcattgc agccggtttc gattacgccg gcatgaccga
     6901 tggcgaactg gtggttgaac gctggttcct gaacgatgaa caagttggcg agtatagcta
     6961 cacctgggaa tggggcgaaa gcggactctt cggcaccttc ctaccgaacg atggcaaccc
     7021 catgcccgct ggaacctatc gcctcgaaat gtacgccgga gatgccatga cccccatggg
     7081 caattcatcc acggtcaccg ttgcgagcgg aggtggtaat agcggtcaac aatctcagcc
     7141 ttcatcaagt ggcgatacca tctcggtcta cggacaagtc tatgatgccg caacgaataa
     7201 tcccatttct ggagcatacg tctttgtact cacctctggc acaacctacg acgaatggga
     7261 aagttctgat tatgccgata aatatataga gacctatctg caaacagact cgaatggcaa
     7321 atataaggtc acagatattt cgcgcaataa agagttcacc ctagtcttcg ccgcccaagg
     7381 ctattacgac gcctcagcgg ataacatgat cgcgaactcc agcgacccag attcgtttga
     7441 gttgaatgtt ggattgaata agtaagtagg ctttg
//
LOCUS       DAOQNJ010000058         7450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5198_length_7450_cov_9.724138, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000058 DAOQNJ010000000
VERSION     DAOQNJ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5198_length_7450_cov_9.724138"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PLG52_02905"
     CDS             <1..1270
                     /locus_tag="PLG52_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB40420.1"
                     /translation="GMILDGTINLTLDGVQGSLAQEKAFDKVLVAVLNACDEDEVCHA
                     DMGGENALAVYDKFAAQVSKDPIEYEYPLPNGEKVKGVFTFNALEFTTAYQMYSLTTR
                     MIFLRALAAANQGDIIPMLRLMYENATIDPATFEYIGDPTFSDTMFLSVLCTDDTFFT
                     GTQEERIQQSIEAGQASNGTVPRLDGSVYTGIDCAFWPSSPKEEVTREPLKLDGVPVL
                     VLNATLDPATPFEEGKFVAENLADGYHIYVEGGVHSIFGYGYACPDQYVTDLLVDGKV
                     PDQREIQCTDWENPVYANYSPNLPASANDFADALDMMIYLDENFYYLPEVYLNDWSEE
                     KSIGCTYGGTYTFAPSEKGEDHTYENCQMMPGVTVNGTGVFDYNAGVFSMAVNISGDK
                     TGELNYSYDFNTGTTSVTGTFEGEPVDLSQ"
     gene            1394..2455
                     /locus_tag="PLG52_02910"
     CDS             1394..2455
                     /locus_tag="PLG52_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNB40421.1"
                     /translation="MQRRSKGVVQLEWVCPNCDTRNPGPVKNCQNCGAPQPDNVQFQR
                     AADEKLITDEKVVQAAKAGADIHCGFCGTRNPATAETCSQCGADLKEGKARQAGQVMQ
                     AAPSTPKVVTCSNCGSENSGSAKTCTNCGAPIKLPQAKAPAPIAASAQAGKVAAQANK
                     KPVNWLVAGGIGAVLVMCCIAVLMLFVFPSRSVTGTVSGVSWQTSVPVQEVQPVNYSN
                     ERGNPPSDAYNVSCRTDSQEVCEDKTIDKGNGYAEVVTECHTVKDQYCSYTVDEWTTI
                     QTYDLNGTDLNPEYSSPNISSDQRLGNSSTQLQVYFSTSDGELTYSPNSVTEFQQYQP
                     GSQWTLKLNAVGSVLSVER"
     gene            2461..2628
                     /locus_tag="PLG52_02915"
     CDS             2461..2628
                     /locus_tag="PLG52_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40422.1"
                     /translation="MILNRTTRNILIAISAGVATGIFLNMLSMSLPSLIVGVVVATGV
                     TFALVHSRKDS"
     gene            2684..3898
                     /locus_tag="PLG52_02920"
     CDS             2684..3898
                     /locus_tag="PLG52_02920"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNB40423.1"
                     /translation="MTKTVAIIGGGPAGLMAAEVLSQNADLQIDVYDAMPSLGRKFLM
                     AGKSGLNLTHSEPFEKFVSRYSRKEVEAWLHAFTPDNLRTWAKELGVDTFVGTSGRVF
                     PVGMKASPLLRAWIQRLSASGVTFHLRHKLSGLPADTSLHFETPNGNVTVKADAVILA
                     LGGGSWRKLGSDGAWVEWLSQAGVKVEPLRPANCGFDVAWSSVFKEKFDGQPVKSVIV
                     SFGAFHQQGEFIITQEGVEGSLVYAASALLRDAIEASGTATMTLDLAPDRTEAQLVER
                     LSKPRGSRSMASHLEKAVGIKGVKAGLLREFVPKEEFTNEARLAFYIKNLPVPLVATR
                     PLDEAISSAGGVRFEMLDQGLMLKHHPGIFCAGEMIDWEAPTGGYLLSACFASGRWAG
                     AGVKKWLSGDME"
     gene            3895..4479
                     /locus_tag="PLG52_02925"
     CDS             3895..4479
                     /locus_tag="PLG52_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40424.1"
                     /translation="MNKNLRILSVLGVQVLIVAALLFGAQKLTEYQNANVPPKTYVYK
                     NIYFDYPGNWELKLEESDQGGELTVTPAKGSPEEKMFGQFYIMFLPDIAFDENWKQTL
                     ADEASCSGKENTQWHQMIEGDDFSGFECVWKDPERAFPSWEFFLYNEGEQTAISILAL
                     PVNDTATEALQSPETVQQLFPDLLALAKSLRISK"
     gene            complement(4559..4933)
                     /locus_tag="PLG52_02930"
     CDS             complement(4559..4933)
                     /locus_tag="PLG52_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40425.1"
                     /translation="MSTCALSVSDDKKFIIMKITGQVNSQDMYQHTLEAHQLGRSLHL
                     RAFLVDLTDAINTQSVVEKYDFVYKKVQEDDELLHFVRMAMWVKKGDHSHDFIETLAN
                     NTGEQAKIFHDQQAAIRFLLEP"
     gene            5280..>7450
                     /locus_tag="PLG52_02935"
     CDS             5280..>7450
                     /locus_tag="PLG52_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB40426.1"
                     /translation="MKFKPISSPLRTALIYLIFGGLWILSSDTVLGLFIHHASNLYPL
                     AQTLKGWLFVLVGAVLLYSILSVDMKALHKREEEIRQSHNLYQQLFDSNPQPMWVYDP
                     ETLHFLMVNHAAVEHYGYTETEFLSMTIKDIRPPEQLAALLANLAKPPQPVESSVGWV
                     HRKKDGSLIDVEISSHAIDFKGRAARMVLANDVTERKKIESALQASEAQFRNLVERTP
                     AITYISTLDEISTTKYISPQIEAVLGFSPKEFEAIPHLWEQQLHPEDHQYTIQTFQKS
                     QKEGRSFICEYRMISKQGEVVWFRDQANLVRNENGKPLFYEGFMLVITEQKLAEQALQ
                     ASEIRFRALFEDVPIAIWEEDFSAVKEHIDLLKTQGVTDFRAYLTAHPNRVHEFMEKA
                     KVLNVNRTALNLYKAASKEELIASTTAELSPGEAENNIEDLIAIAEGRLGNHWEGADT
                     TLKGEKLQINLQWSVVPGHETDYSKVIVTTVDITERKQAEEALRVSEENYRGLVESSD
                     NAIAVFDRDGNVLYANQSSQQLWNAPELVGKTLYDLFPKDFADHHLISMKKVIDEQII
                     DTNELEVVIHGNKMWFHVTMSPLKNSAGSVDRLLLNALNLTERKRAEEAQRRSEILFF
                     KIFRASSIGIHLFRLSDGRSYNVNDAFLEIIGYPREELIGRTAVELNLFEDIETRNRW
                     MQALREGRSVRNQDARIRRKSGEIRDCLASLDLIDVSGEVMV"
BASE COUNT         1995 a         1809 c         1848 g         1798 t
ORIGIN      
        1 tggcatgatt ctggatggca ccattaactt gacattggat ggagtgcagg gttcgcttgc
       61 acaagagaaa gccttcgaca aagtattggt agcggtcttg aatgcctgtg acgaagacga
      121 agtttgtcat gcagatatgg gcggcgagaa tgcgctggct gtatacgata agtttgcagc
      181 tcaagtctca aaagacccga ttgaatatga atatccgtta ccaaacggtg aaaaagtaaa
      241 aggtgtgttc accttcaatg ctcttgaatt caccacggcg tatcagatgt actctcttac
      301 aacccgtatg attttcttaa gagcattggc tgccgcaaac caaggcgaca tcatccccat
      361 gttgcgcttg atgtacgaaa atgcgaccat tgacccggcg acctttgaat acatcggcga
      421 cccaactttc tccgatacga tgttcctcag cgtgctatgt actgacgata ccttcttcac
      481 cggcacccaa gaggaacgca ttcaacaatc gattgaagcg ggtcaggcgt cgaacggaac
      541 cgttccacgt ttggacggct cggtctacac cggcatcgac tgtgccttct ggcctagttc
      601 gcccaaggaa gaagttacac gtgagccgtt gaagttagat ggcgtgcctg ttttggtttt
      661 gaacgcaacc ctcgaccctg ccactccgtt cgaagaaggt aagtttgtgg ctgagaactt
      721 agcagatggc tatcacattt atgtggaagg cggcgttcac tccatcttcg ggtatggtta
      781 tgcctgcccc gaccaatatg tgaccgacct tttagtagac gggaaagttc cagaccaacg
      841 cgagatccaa tgtacagact gggagaaccc cgtgtacgca aactactctc cgaacttacc
      901 cgcaagtgca aatgactttg cagacgcatt ggatatgatg atctatctcg atgagaactt
      961 ctattattta cccgaagtgt atctcaacga ctggtctgaa gaaaaatcta ttggctgtac
     1021 ttatggcggc acctacacct ttgcacccag tgaaaaaggc gaagaccata cctatgagaa
     1081 ttgccagatg atgccgggcg ttaccgtcaa cggcacaggc gtttttgatt acaacgccgg
     1141 cgttttcagc atggcagtca acatcagcgg agataaaacc ggagagctca attactcgta
     1201 tgatttcaat acaggaacca catctgtgac cggcacgttc gaaggagaac cggtagacct
     1261 atcgcagtag aacattaatt gcgatgccta aacaccttcg ataccctcga aggtgttttt
     1321 aatgatgcca ttcaatccat gcacagattg ttgtacactt ggcacatacg caatgattga
     1381 taaggagtag accatgcaaa gaagaagtaa aggcgttgtg cagttggaat gggtatgccc
     1441 aaactgcgac acgcgcaacc cgggacccgt caagaactgc cagaactgcg gcgcgcccca
     1501 acctgataat gtccaattcc agcgagccgc tgatgaaaaa ttgatcaccg atgaaaaggt
     1561 ggtgcaagct gccaaagcgg gcgcagacat ccactgcgga ttctgtggga cgcgcaaccc
     1621 cgccacagcc gagacttgtt ctcaatgcgg cgcggatttg aaggaaggca aagcccgcca
     1681 ggctgggcaa gtgatgcaag cggctccttc cacgccgaaa gttgtcacct gctcgaattg
     1741 cggaagcgaa aactcaggca gtgccaaaac ctgtacgaac tgtggtgcgc ccatcaaatt
     1801 gccacaagcc aaagcgcccg cccccatcgc ggcatctgca caagcgggca aggttgctgc
     1861 gcaggcaaat aaaaaaccag tcaactggtt ggtggcaggc ggcattggcg ccgttttggt
     1921 gatgtgctgt atcgctgttt tgatgttgtt cgtgttccca tctcgttctg tcacgggcac
     1981 agtctctggt gtctcttggc aaacatcggt gcctgtgcaa gaagttcagc cagtcaatta
     2041 ttcaaacgaa cgcggaaacc caccgtccga tgcctataat gtttcctgcc gaaccgatag
     2101 tcaagaagtc tgcgaagaca agaccatcga caaaggcaat ggctacgctg aagtagtgac
     2161 agaatgtcac accgtaaaag accaatattg cagttacacc gtcgacgagt ggacaaccat
     2221 tcagacctat gacctaaatg gaacagacct gaaccctgaa tattccagcc ccaacatttc
     2281 cagcgatcaa cgcctcggga atagctctac ccagttacag gtctacttca gcaccagcga
     2341 tggcgagttg acctattcgc ccaactcggt cactgagttt caacaatatc aacccggcag
     2401 tcagtggacg ttgaagttaa atgccgtggg cagtgtgctc agcgtagaac gataagccct
     2461 atgattctca atagaacgac ccgaaatatc ttgatcgcaa tttcagccgg ggttgcaacc
     2521 ggcatcttcc ttaacatgct atccatgtcg ctgccttcgc ttatcgtggg cgttgtcgtt
     2581 gccacgggtg tgacatttgc gctcgtgcat tctcgcaaag atagttaatc gttatatata
     2641 aagagaaact ccctttcggg agtttctctt tataatgaac cccatgacga aaaccgtagc
     2701 aatcatcggc ggcggaccgg ctggcttaat ggcagcagag gtcttatcac aaaacgctga
     2761 ccttcaaata gatgtatatg atgccatgcc ttcgctgggg cgcaaatttt taatggcagg
     2821 caaaagcggg ctcaacctta cccactctga accttttgaa aaattcgttt cgcgctacag
     2881 ccgcaaagaa gtcgaagctt ggctgcatgc cttcacgccc gataatttgc gtacctgggc
     2941 aaaagaactc ggtgtagata cgttcgtggg aacttcaggg cgcgtctttc cggttgggat
     3001 gaaagccagc ccactactgc gggcgtggat tcaaagactc agcgcctctg gcgtgacctt
     3061 tcacctgcgc cacaaattga gcgggttgcc agccgatacg tctctacatt ttgaaacacc
     3121 caatggcaat gtcaccgtga aagctgatgc ggtcatcctt gctttgggcg gcggcagttg
     3181 gcgcaaactc ggctcggacg gggcatgggt cgaatggttg agtcaagccg gggttaaggt
     3241 tgagccgtta cgtccagcca attgcgggtt tgatgtggca tggagttcgg tattcaaaga
     3301 aaagtttgac gggcagccag tcaagtctgt gatcgtatcg tttggggcgt ttcatcagca
     3361 gggcgaattt atcatcacac aagaaggcgt ggagggaagt ttggtatacg ccgcttcagc
     3421 attgttacgt gatgcgattg aagcaagcgg tacagccaca atgactctag accttgcacc
     3481 agaccgaaca gaagctcagt tggtagagcg gttatcaaaa ccacgtgggt cgcgctcgat
     3541 ggcaagccat ttggaaaaag ccgtaggcat aaaaggggtc aaagccgggt tgttgcgtga
     3601 gttcgtaccc aaagaagaat ttacaaatga agcacggctg gctttctata tcaagaacct
     3661 gcctgtgcca ctggttgcga ctcgtccgtt ggatgaggcg attagttctg ctggcggcgt
     3721 gagatttgag atgttggatc aaggcttgat gctaaaacat caccccggca ttttttgcgc
     3781 cggtgaaatg atagattggg aagctcccac gggcggttat ttgctgagcg cttgttttgc
     3841 aagcggacgt tgggcaggtg ctggcgtaaa aaaatggtta tctggagaca tggaatgaat
     3901 aagaatctta gaattttgag cgtgcttggg gtacaagtgt tgatcgttgc ggcattattg
     3961 ttcggcgcac aaaagttgac tgagtatcag aatgcaaacg tcccacccaa gacctatgtg
     4021 tataagaata tctattttga ttaccccggc aattgggaac tcaagttgga agaaagcgac
     4081 caaggcggcg aactgaccgt cacacctgcg aagggctcgc cggaagaaaa aatgtttggg
     4141 cagttctaca ttatgtttct gcccgatatt gcctttgatg aaaactggaa gcaaactctg
     4201 gcagacgaag ccagttgttc tgggaaagaa aacactcagt ggcatcagat gatcgaaggg
     4261 gatgatttct cgggctttga gtgtgtgtgg aaagaccccg aaagagcctt ccccagttgg
     4321 gagtttttcc tctacaacga aggtgaacaa accgccatca gtattctagc cttgccggtc
     4381 aacgatacgg caaccgaagc tctacaaagc cctgaaactg ttcagcaatt gttcccagac
     4441 ctgctggcgc ttgcaaaaag tttacgcatt tctaagtagt caatttttgt cttgtctatc
     4501 aaaaggacat ccctcgatat ggctcgaggg atgtcctttt tgatggatgg agcacgttct
     4561 acggttcaag taaaaagcgg atagccgctt gttggtcgtg aaatattttg gcttgctccc
     4621 ctgtgttgtt ggcaagggtt tcgataaaat catgggaatg gtcgcccttt ttcacccaca
     4681 tcgccatacg aacgaaatga agcaactcgt catcctcctg aactttttta tagacaaaat
     4741 catatttttc gactacggat tgggtattga tggcatcggt caggtcgacg agaaaggcgc
     4801 gcagatgaag ggaacgcccc aattgatgcg cctccaatgt atgctggtac atgtcctgac
     4861 tattgacctg cccggtaatt ttcatgatga tgaatttttt atcatctgaa acagaaagcg
     4921 cacaagtgct catagtggtc tcctatccct attaagacct acttccattg tattttatat
     4981 ttggctacgt ttcaatcaat tttctctatg acttgtatac attcctagct ttacattgta
     5041 acctactttg ccaattgttc aaattttggt aaaaccagct ccaaaccaaa agagacagtc
     5101 ccgtagcagg ggctgtctct tggtttaagg gtggaatagg aggtcaaatc gtcaacagga
     5161 ggtcaactta cactataatc ttgttactta gtaacaatgt caggcaaagc ctgaagtgct
     5221 atactttttt tattggttaa cttttagagg tttaacattt tgttgcatgg aggtagtttg
     5281 tgaagttcaa gcctatctca agcccactac gaaccgcact gatctacctg atctttgggg
     5341 gactatggat tctttccagt gataccgtat taggtctttt tatccatcac gcatcgaatc
     5401 tctacccact tgcacaaacc ctcaagggct ggcttttcgt gctggtcggt gccgtgctct
     5461 tgtattcgat cttatctgta gatatgaaag ccctacacaa gcgcgaagag gaaatcaggc
     5521 aaagccacaa cctatatcaa cagttattcg atagcaaccc acagcctatg tgggtctatg
     5581 accctgaaac gctacacttc ttaatggtga atcacgctgc agtagagcac tatggctata
     5641 cagaaactga attcctgagc atgaccatca aggatattcg cccgccggag cagttagctg
     5701 ccctgctggc aaacctggca aagccgccgc aacccgtgga aagctctgtt ggctgggtgc
     5761 accgcaagaa agacggctcc ttgatcgatg tggaaatctc ctcgcacgcc atcgacttta
     5821 aaggcagagc ggctcgcatg gtcttggcaa atgatgtcac cgaacgaaaa aagatcgaat
     5881 ccgccctgca agcctcagaa gcccagtttc gtaatctggt cgaacgcacg cccgcgatca
     5941 cctacatttc caccctcgac gaaatcagca ccacaaaata catcagtcca cagatcgaag
     6001 ccgtattggg gttctctccc aaagaatttg aagcgatccc gcatttatgg gagcaacaac
     6061 tccacccgga agatcatcaa tatacaattc agacctttca aaagagtcaa aaagaggggc
     6121 gttcttttat ctgtgaatat cgcatgatca gcaagcaagg cgaggtcgtt tggttccgag
     6181 accaagccaa cttggtcagg aatgaaaatg gaaagccgct cttttatgag ggctttatgt
     6241 tggtcatcac cgaacaaaag ctggcagaac aagccctaca ggcgagtgaa atccgcttcc
     6301 gcgccttgtt tgaagatgtg cccattgcca tttgggaaga agatttttca gcagttaagg
     6361 aacacatcga tcttctaaaa acacaagggg ttactgattt ccgagcctat ttaacagccc
     6421 accctaacag ggtgcatgag tttatggaaa aggcaaaggt gttgaacgtc aaccgcaccg
     6481 cgcttaacct gtataaggca gccagcaaag aggagttaat cgccagcaca accgcagaac
     6541 tcagccccgg cgaagcggaa aataatattg aagacctcat tgccattgcc gaaggcaggc
     6601 ttggcaatca ctgggaagga gccgatacca ccctaaaagg cgaaaagctc cagatcaacc
     6661 tgcaatggtc tgtggtgccg gggcatgaaa ccgactattc caaagtgatc gtcaccaccg
     6721 ttgatatcac tgaacgcaaa caagccgaag aagccttgcg cgtgagtgaa gaaaattatc
     6781 gcgggttggt agagtcttct gataacgcga ttgccgtgtt tgatcgagac ggcaatgtgc
     6841 tttatgcaaa ccaatccagc cagcaattat ggaacgcccc cgaactggtc ggcaagaccc
     6901 tgtacgacct cttcccgaaa gactttgcag accaccatct catttccatg aaaaaggtca
     6961 tcgatgaaca gatcatcgac accaatgaac tggaagtcgt gatccatgga aacaagatgt
     7021 ggttccatgt caccatgagt ccgctaaaaa attcagccgg ctctgtggac aggctgttgt
     7081 taaacgcgct caacctcacc gagcgcaaaa gagccgagga agcgcaacgc cgatcagaga
     7141 ttctattctt taagatcttc cgtgccagtt ccatcggcat ccatctcttc cgtctttccg
     7201 atgggcgctc ctataatgtc aatgatgcgt ttcttgagat catcggttac cctcgcgaag
     7261 aattgatcgg gcggactgcc gtagagttaa atctgttcga agatattgag acgcgaaata
     7321 ggtggatgca agccctacga gaaggcagga gcgtccgcaa tcaagatgcc agaatcaggc
     7381 gcaaatctgg cgagattcga gactgcctcg catcgctgga cttgatcgat gtcagcggcg
     7441 aggtcatggt
//
LOCUS       DAOQNJ010000059         7311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5371_length_7311_cov_7.353225, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000059 DAOQNJ010000000
VERSION     DAOQNJ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5371_length_7311_cov_7.353225"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2153)
                     /locus_tag="PLG52_02940"
     CDS             complement(<1..2153)
                     /locus_tag="PLG52_02940"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNB40427.1"
                     /translation="MFTMGLTSFEQIAIWAVFAVAWLGLGYAIFLRSQILNEDKGTEK
                     MQEVWGAIKDGANAYLQKQFKSILPLIGILFIALFLSVYVVPPSPEATERFSGMSESQ
                     VKLIIGLARALAFVMGAGFSLTVGQIGMRMAIEGNVRVAAASKKSFGDALRIAYRAGT
                     ITGMLTDGLGLLGGTIIFIILGIAAPDALLGFGFGGTLLALFMRVGGGIFTKAADVGA
                     DLVGKVEAGIPEDDPRNPAVIADLVGDNVGDCAGMAADIFESYEVTIVSGLILGLALW
                     HATGRLEWIVFPLLVRGIGVLSSIIGTYFVKAGKTGKSEDAMSAIFSGFLTSAVISAA
                     LFFIAGFLYMGKTMAEYGGWWRMPTAVGVGVLLAILIDRLTEYFTGTHASPVNDIKKS
                     ADTGPATLILNGITVGFESSVWSVLVIALTIIASIFIFGTIPGVEGATRATFILYGVA
                     MTGIGMLTLTGNNVAMDSFGPIADNANGIGEMSWSGKTDKATKDAQQIMADLDAVGNT
                     TKAITKGVAIGSAVIAAVSLFGSFLVDVSRAQSSLGVALADQIQSIGIRVDIPQIFVG
                     MLIGGALPWLFSSFAIQAVTRAASLIVLEVRRQFKLGVLDGKVKPDYAQAVGISTTAA
                     QKELVSLALLGIVTPIVVGLLLGVEALGGFLAGIIVSGQLLAVFLNNAGGAWDNAKKL
                     IEDEPKDPAKNLGKGSERHKAGVVGDTVGDPFKDTA"
     gene            2346..2756
                     /locus_tag="PLG52_02945"
     CDS             2346..2756
                     /locus_tag="PLG52_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNB40428.1"
                     /translation="MLQEINTQLFTLTPAASEAVKNILTERKLDGYALRVYVAGGGCC
                     GVNFGMALDNNFRDVDTTFEANGVKVVVDEVSIDYLRDATVDFVNDPMRGAGFAVNSP
                     NAQGHSHGEGGCACGAGGGESSCGCGGGSCSCAN"
     gene            complement(2813..3439)
                     /locus_tag="PLG52_02950"
     CDS             complement(2813..3439)
                     /locus_tag="PLG52_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40429.1"
                     /translation="MFDLRKRLIAFLFIIAFGMWMAFYQPTQNLVVAQQPTGSIPTVT
                     GTPPGMIVRVNIDQNFEIVPVHSGPNSTIYPVVGVILMGQEIPAVGISEDQDWIQIYY
                     PGVPGSTAWVFGPYVTIIKTGRLPVIPAPNTPTPVSTPTINPTLVAAFVDPVTPTRLP
                     TYTPPPEFVVPTFLPDTGSENRVPVGLFIIALGFIGGFGTLISFLRGR"
     gene            complement(3448..4059)
                     /locus_tag="PLG52_02955"
     CDS             complement(3448..4059)
                     /locus_tag="PLG52_02955"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNB40430.1"
                     /translation="MTKYKFHPLVLTLTFLLLVVWIFNVSSPTNVHAQQPTDTPASAT
                     GLFVTVVTNEPQINVRMGPSATVYPVIGVLLTGSTVPALGRSKGGDWVQIEFASGPNG
                     KGWVYSPLVEVSPAGSTLIVVDAPPTPVQPATSTIDPTLAAQFIIEPTSTHLPTFTPA
                     PTLIKVQFEPSASLSYSIPWASIIIALAVLGVIGFLLSLVIRR"
     gene            4109..4693
                     /locus_tag="PLG52_02960"
     CDS             4109..4693
                     /locus_tag="PLG52_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNB40431.1"
                     /translation="MERKIFHGNIKPVDIAQALLAEFNQGNMRAQILGQSDKMVVQVG
                     TRPDAMSGGQTAMTVTIQTLEEGIMVELGQQAWLGVAASLGATALAAIRNPFSLLGRL
                     DDIAQDIENLQISERVWAVISKAARAAGASAELSENLKRTTCEYCHVANPVGQPSCSA
                     CGAPLGNVQPTTCRHCGFVVRPNEKTCPNCQKPV"
     gene            4706..5530
                     /locus_tag="PLG52_02965"
     CDS             4706..5530
                     /locus_tag="PLG52_02965"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
                     /protein_id="HNB40432.1"
                     /translation="MNLREIEARLQTLIEVDLLKVLPGKRVEDVIVLKLATALQQNAI
                     QQDGTTVLPDVYTLILHPETAKAWEDEPFLTVLLNSVATVAQEAGFKFTIPPIITVIR
                     DENIPLNDVEIKTSHRIETMAETNATPLDTGSLEATGEKLPENSFLIVEGVKIYPLNL
                     PVVNIGRRLDNQLVIDDPRVSRNHAQLRAIKGRFVVFDLNSTGGTFVNGQRTSQSVLY
                     PGDVISLAGVALIFGQDNPPPRPDLKDTNPYKMTSDRPTAVFNKPDHSTAEFKKKK"
     gene            5527..6006
                     /locus_tag="PLG52_02970"
     CDS             5527..6006
                     /locus_tag="PLG52_02970"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB40433.1"
                     /translation="MSGSVVLALRILTALAMYGFLGWVLVFLYREVQRQGIVLANRRV
                     PGISIMVTPKGGAGTLRYFHQAEITIGRDPGCDIPLPEDTISARHAHLTYHHNQWWLE
                     DLASTNGTRLNEMRITTPTVITSGDEIQCGGSNLSVSLSENVIIEPTIRLKNKPDSQ"
     gene            6003..>7311
                     /locus_tag="PLG52_02975"
     CDS             6003..>7311
                     /locus_tag="PLG52_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNB40434.1"
                     /translation="MNNQVQSRLLQWSALFLFLQSIILTLAPAVRERTWDVDYRLSHW
                     FGFLIWCIFTYIAHRTLNHYLPEKDPYIFPAATLLSGWGLLTVWRLDESFCIRQAAWF
                     ATSIIVLILAVRYFRSLSFLRNYKYVFLIGGLLITALTLIFGTNPMGAGPRLWLGLGG
                     VYFQPSEPLKILLVIYLSAYLAERSNIRLSSLPLLIPTLLVTGVALLLLVVQRDLGTA
                     SIFIFLFTIFIFIVTGKRRILIGTGSFLLIALLIGYFLIDIIHVRVVGWLNPWDDPTG
                     NSYQIIQSLLAVANGGTLGRGLGIGSPLLVPVAISDFIFAAIAEETGLLGTLGLIATI
                     WLILARGLTIALRAPDNFRRYLAAGIVAYLGVQSLLIIGGNLRLLPLTGVTLPFVSYG
                     GSSLLTSFIALYLLLSISNVEDDEPATLSNPAPYSILAGIFALG"
BASE COUNT         1934 a         1822 c         1814 g         1741 t
ORIGIN      
        1 gccgtatcct tgaaaggatc accgacggta tcacccacca caccagcctt gtggcgttca
       61 gaacccttac ccagattctt ggcaggatcc ttgggttcgt cttcgatcaa cttcttggca
      121 ttatcccaag cgccaccggc gttgttaagg aacaccgcca acaactgacc agagacaatg
      181 ataccggcga ggaaaccgcc gagggcttcc acacccagca acagaccgac aacgatcggg
      241 gtcacaatgc caagcaatgc gagagaaacc aattccttct gagccgcagt ggtggaaatt
      301 ccaaccgcct gagcgtaatc aggcttgact tttccatcca acacacccag tttgaactga
      361 cggcggactt ccaacacaat gagggaagca gcacgagtca cagcctgaat ggcaaaggaa
      421 gagaacagcc acggcaaagc gccaccgatg agcataccca caaagatctg cgggatgtcc
      481 acgcggatac caatggactg aatctggtca gcaagggcaa cacccaagct cgactgagcg
      541 cgggacacat ccacaaggaa ggagccgaag agcgagaccg ctgcaatcac agcagaaccg
      601 atggcaacac ccttggtgat ggctttggtg gtattgccca ccgcatcgag gtcagccatg
      661 atttgctgcg catctttcgt cgccttgtct gttttgccag accaagacat ttcgccaatg
      721 ccgtttgcgt tatctgcaat cggaccgaag gagtccatcg caacattgtt accggtcagg
      781 gtcaacatac cgataccggt catggcgaca ccgtaaagga tgaaggtcgc gcgggttgca
      841 ccttcaacac caggaatggt gccgaaaatg aaaatggaag caatgatggt caacgcaatg
      901 accaacaccg accacacaga agattcgaaa ccaacggtaa taccgttcag gatcaaggtc
      961 gcaggacccg tatccgcaga tttcttgatg tcgttcacag gggaagcatg agtgccggtg
     1021 aagtattcgg tgaggcggtc gatcaagatc gcgagcaaca cacctacacc gacagccgtc
     1081 ggcatacgcc accaaccacc gtattcagcc atggtcttgc ccatgtaaag gaagccagca
     1141 atgaagaaca aagcagcgga gatcacagca gaggtcaaaa agccgctgaa aatggcgctc
     1201 atggcatctt cactcttgcc ggtcttgcca gccttcacga agtaggtacc aatgatggaa
     1261 gaaagtacgc cgataccacg caccaacaag gggaagacga tccattcgag gcgacccgta
     1321 gcatgccaga gagccaagcc gaggatcaag ccagaaacga tggtcacttc ataggattcg
     1381 aagatgtcgg cagccatacc agcgcagtca cccacgttgt caccaacgag gtccgcgatc
     1441 acggcagggt tgcgtggatc atcctcaggg atgcccgctt cgactttacc gacgaggtca
     1501 gcaccgacat cggcagcctt ggtaaagata ccaccgccca cacgcatgaa caacgcgagg
     1561 agggtgccac caaagccgaa gcccagcaga gcatcggggg ctgcgatacc aaggataata
     1621 aagatgatgg taccgccgag cagaccgaga ccatcggtga gcataccggt gatggtgcca
     1681 gcacggtaag caatacgaag cgcatcgcca aacgacttct tggaagccgc cgctacacgc
     1741 acatttcctt caatcgccat acgcataccg atctgaccta ccgtcagtga gaagcccgca
     1801 cccatgacaa atgccagagc acgtgcaaga ccgatgataa gcttgacctg gctttcgctc
     1861 ataccgctga agcgttcagt cgcttcagga gatggcggaa ccacgtacac ggaaaggaac
     1921 aaggcaatga agaggatgcc gatcaaaggc aagatggatt taaattgctt ctgcaaatag
     1981 gcatttgcgc catctttaat cgcaccccaa acttcttgca ttttttccgt tcccttatcc
     2041 tcattgagga tctgggagcg caaaaaaatc gcgtatccga gcccaagcca tgcaacggca
     2101 aaaacggccc aaatcgcgat ctgttcaaaa ctggtcaatc ccattgtgaa catgaaagga
     2161 tactcctcca acagagatga aaaaataaag gctgacgcct ttttaaaagc acagagattt
     2221 tacagtcgca tagtctttgt gtcaagaaaa tgagtttgga ttaatcttca tacaaaaaaa
     2281 gttgacagcc cactttaaac ctgataacat ttaccaaaat tagatgtaac acaaggagaa
     2341 accttatgct ccaagaaatt aacacccaac tttttacact tacccctgct gccagcgaag
     2401 cggttaagaa tattttgacc gaacgcaaat tagacggcta cgcgttgcgc gtgtacgttg
     2461 ctggcggtgg ttgctgcggc gtcaactttg gtatggcgct tgataataat ttccgtgatg
     2521 tggataccac cttcgaagcc aatggcgtca aggtcgttgt tgatgaagtt tccatcgatt
     2581 acttacgcga tgcgactgtt gatttcgtca acgacccgat gcgtggcgct ggtttcgctg
     2641 tcaatagccc gaacgctcag ggacacagtc acggcgaagg cggttgcgcc tgcggtgctg
     2701 gtggcggtga aagctcttgc ggatgcggcg gcggttcttg cagctgcgca aattaatcta
     2761 agatcgttaa atcaaaagga caggtcatag acctgtcctt ttttgttcct ttttatcgac
     2821 cgcgtaaaaa tgagatcaag gtgccaaacc cgccaataaa cccgagggca atgatgaata
     2881 agccaacagg cacacggttt tcggaacctg tatctggcag gaatgttggg acgacgaatt
     2941 ctggcggcgg tgtgtaagtt ggtaaacgcg ttggggtgac ggggtcaaca aaagcagcaa
     3001 ccagagtagg gttaattgtg ggtgttgaaa caggcgtcgg cgtatttgga gccggaatga
     3061 ctggtagtcg tcctgttttg atgatggtga cgtatggtcc aaaaacccag gcggttgagc
     3121 cgggtacgcc gggataataa atttgaatcc agtcttgatc ttcagaaatg cctacggctg
     3181 gaatctcttg ccccataaga ataaccccaa caacggggta gatcgttgag ttcggacctg
     3241 aatgaacagg tacaatctcg aaattctggt caatgttcac acgtacgatc atgccaggag
     3301 gtgtaccggt tacggttgga attgaacctg tgggctgttg tgcaacgaca aggttttgtg
     3361 ttggctggta gaacgccatc cacatcccaa atgctattat aaatagaaag gcgatcaaac
     3421 gttttctaag gtcgaacatg gtcaatttca acgacggatc accaaagaaa gtaaaaagcc
     3481 aatcacaccc aagaccgcca atgcaataat gatcgaagcc cacggaatgc tatagctcaa
     3541 gctggcagat ggttcaaatt gaactttgat gagggttggt gcaggggtaa acgtcggcag
     3601 atgtgtgctg gttggctcaa taatgaattg cgcggcaaga gtcgggtcaa tagtggatgt
     3661 ggcaggttgg actggtgttg gtggagcgtc tacaacaatg agggtgctac ctgctggtga
     3721 gacctcaacc agcggggaat atacccagcc ttttccgtta ggtccggatg caaattcgat
     3781 ctgaacccag tcgccgcctt ttgaacgccc caaagctggg actgttgagc cggttaataa
     3841 cacaccaatc acgggataca cggttgcgct gggacccatg cgaacattga tctgcggttc
     3901 attggttaca acagtaacga agagaccggt tgcagaggcg ggagtatccg tcggttgttg
     3961 agcatggaca ttagtcgggg aagagacgtt gaatatccaa acaaccaata aaagaaaggt
     4021 taacgtcagt acgaggggat gaaacttata ttttgtcatt tcgtaaagtt tagaaaggag
     4081 aatcgcaaag gcgattataa tcgagttcat ggagcgaaaa atctttcacg ggaatatcaa
     4141 gccggtggat attgcacagg ctttgctggc agagttcaat caggggaata tgcgtgcgca
     4201 gatcttgggt cagagtgaca agatggttgt gcaggtgggc acgcgcccag atgctatgtc
     4261 tggcgggcag accgcgatga ctgtaacgat ccaaacactt gaagaaggca tcatggtgga
     4321 gttgggtcaa caagcttggc ttggagtggc tgccagcttg ggtgccactg cgcttgccgc
     4381 tattcgaaat ccgttcagtc tattagggcg cttggacgac attgctcaag acattgaaaa
     4441 tttacaaatc agcgaacgtg tttgggcggt tatctccaag gctgctcgtg cggctggtgc
     4501 atctgccgaa ctctctgaga atttgaaacg cactacttgt gaatactgcc atgttgccaa
     4561 cccagttggt cagccttcct gcagcgcttg tggagctccg cttgggaacg tgcaacccac
     4621 tacgtgccgt cactgtggtt ttgttgtaag acctaatgag aaaacctgcc cgaactgtca
     4681 gaaacctgta taactaaaaa ccaaaatgaa tttacgagaa atcgaagcac gtctccaaac
     4741 cttgatcgaa gtagaccttt taaaggtttt gccgggcaaa cgcgtggaag atgtgattgt
     4801 cttaaaactc gcaacagcgc ttcaacaaaa tgcgatccag caagatggaa cgactgtttt
     4861 accagatgtc tacacgctca tattgcaccc tgaaaccgca aaagcctggg aagatgagcc
     4921 atttcttaca gttctattaa attctgtggc aactgtagcc caagaagccg gatttaagtt
     4981 tacaataccc cctatcatta ccgtcataag ggatgaaaac atccctttaa acgatgtaga
     5041 gatcaaaaca tctcaccgga tagaaacgat ggcagaaaca aacgcaaccc ccctcgatac
     5101 tggcagcttg gaagcgactg gcgaaaagtt acccgaaaat tcattcttaa ttgtagaagg
     5161 cgtgaagata tacccgctaa atcttcccgt ggtcaatatt gggcgccgac tcgataatca
     5221 acttgtgatt gatgatccgc gtgtctctcg caaccatgcc caactccgcg cgattaaggg
     5281 acgtttcgtt gtattcgacc tcaactccac cggcggcacc tttgttaacg gtcaacgcac
     5341 cagccaaagc gtgctttacc cgggcgatgt aatctcactg gcgggcgtgg ctctcatttt
     5401 tgggcaagac aatccaccac cgcgcccaga cctcaaagat accaacccct acaaaatgac
     5461 gtctgaccgt ccaaccgccg tattcaataa acccgatcat tccacagctg aattcaagaa
     5521 gaagaaatga gtggttctgt tgttctggcg ctgaggattc tgacagcgct tgcaatgtac
     5581 ggctttcttg gctgggtgct tgtattcttg tatcgtgaag ttcaacgcca gggaattgtg
     5641 ctggcaaatc ggcgcgtccc cggcattagt attatggtga cacccaaagg cggggcggga
     5701 acgctgcggt actttcatca agccgaaatc accattgggc gcgaccctgg ctgtgatatc
     5761 cccttacctg aagatacaat ttctgcacgg catgcacacc tcacctacca tcataatcag
     5821 tggtggttag aagaccttgc ctccacaaat gggacacggc taaatgagat gcgtatcact
     5881 acaccaacag tgatcacctc tggagatgaa attcaatgcg ggggttcgaa tttatctgta
     5941 agcctttctg aaaacgtcat catcgagccg acaattcggt taaaaaacaa acctgattcc
     6001 caatgaataa ccaagtacag agccgtctgc tgcaatggtc ggcacttttt ctatttctac
     6061 aatccatcat cttaacgctt gcgcccgctg tgcgtgaacg aacatgggat gtagattatc
     6121 ggctttccca ttggttcggg tttttgattt ggtgtatctt cacttatatt gcccatcgca
     6181 cacttaatca ttatctccct gaaaaagatc cttacatctt cccggcagct acattactaa
     6241 gcggctgggg tctactaact gtatggcggt tagatgaaag cttttgcatt cgtcaggcag
     6301 cttggtttgc aaccagcatc attgtattaa ttctcgccgt tcgttatttt cgaagccttt
     6361 catttttaag aaactataaa tatgtgttct taatcggtgg gctgcttatc acagcgctca
     6421 cattgatctt tggcaccaac cccatgggag cgggtccacg cttgtggctt gggctgggcg
     6481 gggtatattt tcaaccatcc gagccgttaa aaattcttct cgtcatttac ctttctgcat
     6541 atcttgcaga acgaagtaac atccgcctgt cctctttgcc attactgatc cctactttgc
     6601 ttgtcactgg cgtagctctt ttattattgg tggtacaacg cgacctcggc accgcttcta
     6661 tttttatctt cctcttcacc atttttattt ttatcgtaac aggcaaacga agaatactga
     6721 tcggcacagg ctctttttta ctaattgctc ttctgattgg ttattttctg atcgacatca
     6781 ttcacgttcg ggtagtgggc tggctcaacc catgggatga ccccaccggc aattcgtatc
     6841 aaatcattca atctctttta gccgtggcaa acggcggcac attgggaaga ggacttggaa
     6901 ttggcagtcc gctacttgtg ccggtggcga tttccgattt tatttttgcc gccatcgcag
     6961 aagaaacggg tctactcggg accttggggc tgatcgccac catttggctg attctcgctc
     7021 gcggcttgac cattgccttg cgggcgccag ataactttcg gcggtattta gcggctggga
     7081 ttgtggcata cctcggtgtg caaagcctgc tgatcattgg tggaaattta cgtctgcttc
     7141 cgcttacggg agtgactctg cccttcgttt cctatggcgg ctcttcttta ttaacttctt
     7201 tcattgcctt gtatctgtta ctttctatca gtaatgttga agatgatgag cccgcaacgc
     7261 tttcgaaccc ggctccatat tccatcctag caggcatctt cgcccttggc t
//
LOCUS       DAOQNJ010000060         7217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5506_length_7217_cov_7.729964, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000060 DAOQNJ010000000
VERSION     DAOQNJ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5506_length_7217_cov_7.729964"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLG52_02980"
     CDS             complement(<1..380)
                     /locus_tag="PLG52_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40435.1"
                     /translation="MKLRHHRTWLVLLALIIIGLAGYGATAGTPPFVEASYNGTSLPY
                     QGAGGDPALLDELQQEIDLAAINQIPLSELKTILPQTDAILYRGYVRHNLLRRGLNGF
                     QVCAHASPFASGFLQTQDGREIVCA"
     gene            complement(443..691)
                     /locus_tag="PLG52_02985"
     CDS             complement(443..691)
                     /locus_tag="PLG52_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40436.1"
                     /translation="MQKPKTKFDQKWKLIRGHSMEWWSLIAEHDLKKVDKADDKLDKF
                     LTILQVKYGYTRVEARDEVNRRWTAYSRAKTIVESLAS"
     gene            945..1490
                     /locus_tag="PLG52_02990"
     CDS             945..1490
                     /locus_tag="PLG52_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40437.1"
                     /translation="MEEQQLMEYFKFDASDLNANRNGTLTEKQKTRLNAELREARKSR
                     TRWAYFLFFLAAIGIAIALGIWFIPDSGLGMRIGFGIGFGLVWPALYGLVGFALLPPA
                     SYTDLDLASQTGRVNIVRVESRDPDTRAVSSRYDLYIGPRRFLVDSNIGHVLIQGDEY
                     TIYYLKNSNKIVSAEFISRAK"
     gene            complement(1532..2197)
                     /locus_tag="PLG52_02995"
     CDS             complement(1532..2197)
                     /locus_tag="PLG52_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40438.1"
                     /translation="MFKSKRRQIITPQSEHLKLVGTLAMLWGNDQFDTPPIERNSMVM
                     GMGLHDRGYGYLDNAPIGALRDEEWEGIARRTFTMECSDVIADTIVKYHFRRLAGYGS
                     SEIRKKLHADFTEGIHSQLERHNLSKALFDRTDRITDLLDTLSFEFCFDVPASGSVSV
                     FPRNGEETEVSIEYHVENGEINVHPWTFSVDEYRGYLIAYHAEGYPERLDPCILPYVL
                     KRK"
     gene            2319..2735
                     /locus_tag="PLG52_03000"
     CDS             2319..2735
                     /locus_tag="PLG52_03000"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNB40439.1"
                     /translation="MSEVNDWNKKIIAEFRANGGKVGGNFEGRPLLLLHHKGAKSGQE
                     RVNPVACVQDGERYVVIASKGGAPTNPDWYYNVVANPLTIVEVGTETLKVQATVAEEP
                     ERTRLYNKMAEIMPGFNEYREKTTRVIPVIVLTPVK"
     gene            2744..3250
                     /locus_tag="PLG52_03005"
     CDS             2744..3250
                     /locus_tag="PLG52_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40440.1"
                     /translation="MEAIHSERLTLRPLGLDDAGFIFELVNEPSFIQNIGDRGVRTLA
                     DAEKYIENGPLASYAKNGFGLLAVTLKETGEPIGMCGLIKRDTLEDVDIGYAFLPKFW
                     LKGYAVEAARTVMGTAKALGLKRVVAIVDPANTGSIRVLERLGMSFEKMVRLTADDIE
                     LKLFSVAL"
     gene            complement(3305..4243)
                     /locus_tag="PLG52_03010"
     CDS             complement(3305..4243)
                     /locus_tag="PLG52_03010"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNB40441.1"
                     /translation="MENPTSDEKILAALAHASIILMFLGPLGAVIIWAIQRTKSKYVR
                     YHALQAMGYQTFSFWIWMFMMFFFMIILFGFGAIIGVLSSKSPSAMPPDFLFFIQPLF
                     MLVIFGMWGIIFLVGFIGAVFCGLGKEFRYPFIGSWLQTKILNAPTEEEAEKWEDLWV
                     ASICHTTAILMMWGMIMPIIVWFSQKDRSARLKFQSMQAFLYQLIVTVAYFISYAGFF
                     FVYVFGIAIFAASGVMAEPSQTTMPAGFEIGFFIIMAIFMLFWLAWMILYPAYLITAA
                     VAAVRTLRGHDFKYPLLGKFLAKRMSPPESHEVIQA"
     gene            4432..4836
                     /locus_tag="PLG52_03015"
     CDS             4432..4836
                     /locus_tag="PLG52_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40442.1"
                     /translation="MNYRLLATINAVLLAVVGLIFLVVPVLALDFFNTETYTATSLVA
                     RFLGSAMLLAGMFIWVAKDLTDAGTVRTMTIMLLVSSLVSFILTLVGMVGVDVIRANG
                     WIPLVVQILFVLGYGYLVSGVTITATVQPKKK"
     gene            complement(4909..5169)
                     /locus_tag="PLG52_03020"
     CDS             complement(4909..5169)
                     /locus_tag="PLG52_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40443.1"
                     /translation="MRNGVCTACGSHNVYKSANNSWYQSGVKVAVLGDKVNEVFMTEA
                     YVCLDCRHLELVVEENAAAIFGKGKAFKDAVPASTNWKKVSG"
     gene            complement(5200..5364)
                     /locus_tag="PLG52_03025"
     CDS             complement(5200..5364)
                     /locus_tag="PLG52_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40444.1"
                     /translation="MSTKPRPVVFILIILAAAGLWQFSNGVRSVDVVGLFASGALAGA
                     AISSLFMAQK"
     assembly_gap    5458..5557
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5652..5798)
                     /locus_tag="PLG52_03030"
     CDS             complement(5652..5798)
                     /locus_tag="PLG52_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40445.1"
                     /translation="MNSTPEKTQVKHKRPSKGVRKHNRKVKQEARRVSVPGAEVKKSK
                     RSAS"
     gene            complement(5808..6038)
                     /locus_tag="PLG52_03035"
     CDS             complement(5808..6038)
                     /locus_tag="PLG52_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40446.1"
                     /translation="MNKSLFEEKWKLLRGEVNTRWSLMVEYDLLKVDKAEVKFDKFVT
                     MLQVKYGYTRQKAKEEIVKLWSDYESNHRSKN"
     gene            complement(6154..6408)
                     /locus_tag="PLG52_03040"
     CDS             complement(6154..6408)
                     /locus_tag="PLG52_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40447.1"
                     /translation="MQARTCSKCGSHNVYKNTGKNWHQDGVVLQMIAVDRFTYRFETD
                     AYLCLDCRYLEILVSETNTMYGDQKTLTEVVQASNNWAKA"
     gene            complement(6717..>7217)
                     /locus_tag="PLG52_03045"
     CDS             complement(6717..>7217)
                     /locus_tag="PLG52_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40448.1"
                     /translation="GWIFMVRSKAYRAEAILCVAAIFLYVLMFSGYYMWWGGLAFTPR
                     HLIPILPLFVLPLAFVPDILLIPLILSMVISIFQNLVLTASGFDGLGVYLSEHLRPLW
                     KKHGILQPRGILVYDICLPNVLKGNLINNRGIDLFNLSGPYSLIPLMIAECSFLFIFL
                     KLRFQK"
BASE COUNT         1876 a         1543 c         1770 g         1928 t
ORIGIN      
        1 gcacaaacga tctcacgacc gtcttgcgtt tggagaaatc cgctggcaaa ggggctggca
       61 tgtgcgcaga cttgaaatcc attcagcccg cgacgcagaa gattgtgacg cacatatcca
      121 cgatacaaga ttgcatcggt ctgcgggagg atggttttta attcacttag agggatttga
      181 ttgatggctg cgaggtcaat ctcttgttgc agttcatcta gaagcgcggg gtcaccgcct
      241 gcaccttgat agggtaggga tgttccgtta tatgaggctt ctacaaaagg gggagtgcct
      301 gccgttgcgc cataccctgc tagtccaata atgatcagtg caaggagtac cagccaggtt
      361 cgatggtggc gcagtttcat gaaggcgaat tataaccaaa gctttaaaaa gaaaaagcag
      421 tctcgtttga gaccgctttt ctttagctcg ctagtgattc aacgatggtc tttgcccgag
      481 agtaggcggt ccaacgtcgg ttgacttcgt cacgtgcttc aacgcgggta tagccatact
      541 tcacctgaag aatggtaagg aatttatcga gcttgtcatc agctttatct accttcttaa
      601 ggtcgtgctc agcgatgaga ctccaccact ccatggaatg cccgcggatg agtttccatt
      661 tttggtcgaa ctttgtcttc ggtttttgca taacgcgctc ctaaaagagt atatgaatga
      721 ttggactcag ttggagtttt caagatttcg tttctggctt attccccagt caataggcaa
      781 aacaaaattg tcaaactcgt tcgcattcat tgtactacgt gatgcataaa aaagcagtga
      841 tttttcaaaa cgattttcag aattgataat tgttggatgt gacgctaatg taccattgcg
      901 aattaagttg agagagataa aaatagttca aaggagaagc acccatggaa gaacaacagc
      961 tgatggaata cttcaagttc gacgccagtg acttgaacgc caatcgtaat ggaaccctta
     1021 ccgagaagca aaagacaagg cttaatgctg aacttcgcga agcgagaaaa agcagaacac
     1081 gctgggcata ttttcttttc tttctagctg ccattggcat tgctattgca ctgggtattt
     1141 ggttcatccc tgattcaggg ttgggaatgc ggatcggatt cggcattggc tttgggctgg
     1201 tgtggcccgc tctatatggg ctggtgggct ttgcgctttt accgcccgcc tcatacacgg
     1261 acctggatct tgccagccaa actgggcgag tcaacattgt aagagtagag tcgcgcgacc
     1321 cagatacccg agctgtgagc tctcggtatg acctgtacat tggtcctcgc agatttttgg
     1381 tggattcaaa tattgggcac gtcctaattc aaggagatga gtacaccatt tactatctca
     1441 agaattcgaa taagatcgtt tcggctgagt ttatctcgag ggcaaaatag aacttaatga
     1501 aaaggtctcg cttgaaatag cgagaccttt tttattttct cttgagtaca taaggaagga
     1561 tacatggatc gagccgttcg gggtagccct cagcgtgata ggcgatgaga taaccgcgat
     1621 attcatccac agaaaatgtc cacgggtgaa cgttgatctc tccgttttca acatgatatt
     1681 caatgcttac ttctgtctct tcgccgttgc gcggaaagac tgatacgctg ccagaggcag
     1741 gaacatcaaa gcagaattca aaggaaagcg tatccaaaag atctgtgatg cggtcggtgc
     1801 ggtcgaagag agctttcgag aggttgtgtc tctctaattg agagtggatg ccctctgtaa
     1861 aatcggcatg taattttttg cgaatctcag aactgccata cccggcgaga cgtcgaaaat
     1921 gatatttgac gatggtatcc gcgatcacgt ctgaacattc catggtgaag gttcggcggg
     1981 cgatgccttc ccattcttcg tcgcgtagag cgccaatagg agcattatcg agatagccat
     2041 acccgcggtc gtgcagaccc atgcccatta ccatggagtt gcgttcgatg ggcggcgtat
     2101 cgaattggtc atttccccat aacattgcca atgtgccaac cagtttgaga tgttcagatt
     2161 gcggggtgat gatttgtctg cgttttgatt tgaacatact acatctccaa aatttgacta
     2221 taaagtttca tatgcgattc tatcaataaa taattctctt agagtaagag aaaataaata
     2281 gagaccatcg aaacataaaa caccatagga gaattttcat gagcgaagta aacgattgga
     2341 acaagaaaat tattgccgag tttcgtgcca atggcgggaa agtgggcgga aactttgaag
     2401 gcagaccttt gttgctcttg caccacaagg gcgccaagag cggacaagaa cgggtgaacc
     2461 cggttgcctg tgtacaagat ggcgaacgtt atgttgtgat cgcctcaaaa ggcggcgcac
     2521 cgaccaaccc cgattggtac tataacgtgg ttgccaaccc cttaaccatc gtggaagtgg
     2581 gaacagaaac cttgaaagtt caagctaccg tagcagaaga acccgagcgc acccgcctct
     2641 ataacaaaat ggcagagata atgcccggtt tcaacgaata tcgtgaaaag acgacacggg
     2701 ttattcctgt gatcgtgttg acaccagtga agtaaatttt ctgatggaag caatccattc
     2761 ggaaaggctt acgttgcgcc cgctgggctt ggacgatgcc gggtttattt ttgagttggt
     2821 gaacgaacct tcttttattc aaaatatcgg tgaccgtggt gtgcgaactt tagcggatgc
     2881 agaaaaatac atcgagaacg gtccgcttgc cagctatgcc aagaatggat tcggtttgtt
     2941 ggctgttacg ctgaaagaga cgggtgagcc gattggcatg tgtggcttga tcaagcgcga
     3001 taccttggag gatgtggata tcggctatgc ttttttgccg aagttttggt taaaaggata
     3061 tgctgttgaa gcggcacgga ctgtgatggg cacagcaaaa gccctgggat tgaaacgagt
     3121 ggttgcgatt gtggaccctg ccaacacagg ttcgatccgc gtattggaga ggctgggaat
     3181 gtcattcgag aagatggttc gcttaacggc ggatgatatc gagttgaagt tgttttccgt
     3241 cgctttataa aataaaacgg actcaggcga aatgtctgag tccgtttttg attcatggat
     3301 gggtttaggc ttggatcact tcatgcgatt cagggggaga cattcgtttg gcaaggaatt
     3361 tgcccaagag tggatatttg aaatcgtgtc cgcgcaaagt acgcacggcg gcaaccgcag
     3421 cagtgatgag gtaggctggg tagagaatca tccatgcgag ccagaaaagc ataaagatag
     3481 ccatgataat gaagaaacca atttcgaacc cggcaggcat ggtagtttga gaaggttctg
     3541 ccatcacacc gctggctgcg aaaatagcaa ttccaaaaac atatacaaag aagaacccgg
     3601 catacgagat gaaatatgcc acagttacga tgagttgata caggaacgct tgcatcgact
     3661 ggaacttgag gcgggcggag cggtcttttt gtgagaacca gacgatgatc ggcatgatca
     3721 tgccccacat catcaaaatg gcggtggtgt gacaaatgct cgcgacccag agatcttccc
     3781 acttttcggc ttcttcttcg gtgggggcgt tgaggatctt ggtttgcaac caagacccga
     3841 taaatggata gcggaactct ttacccagcc cgcagaagac ggcgccaatg aaccccacta
     3901 gaaaaatgat gccccacatg ccaaagatca ccagcatgaa caggggttga ataaaaaaga
     3961 gaaaatcggg cggcatggcg gatggagatt tgctgctcaa tacgccgata atggcgccga
     4021 acccaaatag gatgatcata aaaaagaaca tcatgaacat ccagatccaa aagctaaagg
     4081 tttggtagcc catggcttgc agcgcatggt agcggacgta ttttgatttg gtgcgttgaa
     4141 ttgcccagat gatgaccgcc ccgagcggac ctagaaacat gagaatgatc gaagcgtgcg
     4201 ccagagcggc aaggattttt tcatcagatg tggggttttc catgggtttc ctcctgcgtt
     4261 gaattgactt gatgatacct ctattctcaa aaaataaaat gtgacattgg gattaaattt
     4321 gacgccatgc aagggaaatg taaggtttgc gcttcaaaat tcctttgcgc tgttttcgga
     4381 gcgttgtata gtagctttgt tctacattca caccgactta aggagaatac aatgaattac
     4441 cgacttttag cgaccatcaa tgccgttctt ctagctgtag tagggcttat atttttggtc
     4501 gtgcccgtgc tggcgcttga tttctttaat accgagacct acacagccac cagtttggta
     4561 gcccggtttt taggctctgc catgcttctg gcagggatgt tcatttgggt cgccaaagac
     4621 ctgaccgatg caggcaccgt ccgcacgatg acgatcatgt tgctggttag ctcccttgtg
     4681 agctttatct tgaccctggt tggcatggtg ggcgtggacg tgatccgcgc gaacggctgg
     4741 attccgctgg tggtgcagat tctgttcgtg ttgggctatg gctatttggt ttctggtgtg
     4801 accatcactg caacggttca gccgaaaaag aagtagatgc cttgttaagc aaaacggtct
     4861 tgggtatttc acccaagacc gttttttgtt taagcctgaa aacgaacatt aaccggatac
     4921 ttttttccag ttcgtgctgg cagggacggc atccttgaag gctttgcctt tgccgaagat
     4981 ggcggcggcg ttttcttcca ccaccaattc aagatgacga caatcgaggc agacgtacgc
     5041 ttccgtcata aacacttcgt tgaccttatc tccaagtact gcaactttga ccccagattg
     5101 ataccaggaa ttgttcgcgc ttttatagac attgtgagac ccacatgccg tacaaactcc
     5161 atttctcatt gctaaagttt ctcctttgat attgataaat tatttctgtg ccataaaaag
     5221 actgctgatg gctgcacctg ccaatgctcc gctggcaaag agaccgacta catccacact
     5281 acggacgccg tttgagaatt gccaaagccc agcagcggct agtatgatca agataaatac
     5341 aacaggacga ggttttgtgc tcatattttt ctcctttgaa ccaataaaga cgatattaac
     5401 tatgattata tagagaagca ggggagtggg tattccctct ttggtcatgt ggaaaatnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntga ttatatagag aagcagggga
     5581 gtgggtattc cctctttggt catgtggaaa ataaaaacgg tctcatcgga taaacctgag
     5641 accgtcctgt tttacgaagc tgaacgctta ctctttttga cctctgcccc tggcacactg
     5701 acgcgtcgcg cctcttgctt gaccttgcgg ttgtgtttgc gtaccccttt ggagggacgt
     5761 ttgtgcttga cctgtgtttt ttctggggtt gaattcatgt ggctccttta gtttttactt
     5821 cgatgattcg attcataatc tgaccaaagt ttgacgatct cttctttggc tttttggcgg
     5881 gtgtacccgt atttgacttg cagcatggtg acgaacttat cgaacttcac ctcagcttta
     5941 tccactttga gcaggtcgta ttcgaccatc aaactccaac gggtgttgac ctccccgcgt
     6001 agcagcttcc atttttcttc aaacagggat ttgttcataa ggcgctcctc ctcaagaaag
     6061 cgcgttttta gggaatacgg gttgggagtt ttgtgatttc gtgcccatcc atctccccca
     6121 tgaataggaa tgaaaaagat tcaaactctt atgtcaggct tttgcccaat tgttactggc
     6181 ttgaacgact tctgtcagtg ttttttgatc tccgtacatg gtgtttgttt cagagaccag
     6241 aatttccaaa tacctgcaat cgaggcagag ataagcatct gtttcaaagc gataggtgaa
     6301 acgatctacg gcgatcattt gcaggacaac cccatcctga tgccagtttt tacctgtatt
     6361 tttatagaca ttgtgggaac cacattttga gcatgttcga gcttgcatgg aaatctcctt
     6421 ctcagatttg agtgtatccg tctcgcttaa tagggcgagt aaggttttct ggaaggtatg
     6481 ttgatgtgat tgcatccccc gttaccgtct agttgcctga tttcattgta cccttttctg
     6541 ggaataaaga tagacaagaa aaaaacggtc tcgcggatat aacatcccga gaccgttttt
     6601 cattccttga atcggcacag ggagtgatga tatttgccaa agaggtgttg gggctgaaat
     6661 acatcgccgc tgcaccttct tcgtacccat tatattcact agtttgcatt tggttcttat
     6721 ttttgaaacc ttaattttag aaagataaat agaaagctac actctgcaat cattaatggg
     6781 attaagctat aaggtcccga taggttgaaa agatcgatgc ctctgttgtt aatcaaattc
     6841 cctttaagta cattcggcag gcagatgtca tacacaagga tccctctagg ttgcaaaata
     6901 ccatgcttct tccaaagagg tcgcagatgt tcactgagat aaacgcccag cccatcaaat
     6961 ccgcttgcgg ttaagacaag gttttgaaaa atggagatta ccatgcttag gatcaaaggg
     7021 ataaggagta tgtccggcac aaatgccaac ggtaatacga ataacggcag gatgggaata
     7081 agatgcctgg gcgtaaaggc gagtccgccc caccacatgt aatagcctga aaacatcagc
     7141 acgtataaaa agatcgccgc aacacacagg atcgcttcgg cacggtatgc ctttgatctg
     7201 accataaaga tccagcc
//
LOCUS       DAOQNJ010000061         7208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5513_length_7208_cov_11.073116, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000061 DAOQNJ010000000
VERSION     DAOQNJ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5513_length_7208_cov_11.073116"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLG52_03050"
     CDS             complement(<1..581)
                     /locus_tag="PLG52_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB40449.1"
                     /translation="MTTEFETFLQKYPTYKHTEKIDDLRKSDYSRLDAGEHVYFDYTG
                     GGIYAESQVKKHYQLLQENVFGNPHSSNPTSIAATHLVEGTREYILKFFNADPDEYLA
                     IFTPNASGALKLVGESYPFPNGRYLLTFDNHNSVNGIREFAHARGAEVTYIPVMLPEM
                     RVDASQLDLELSRPSKAGHNLFAFPAQSNFSSV"
     gene            759..1553
                     /locus_tag="PLG52_03055"
     CDS             759..1553
                     /locus_tag="PLG52_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40450.1"
                     /translation="MALVTRNMKVYFAAVGLLALWVGAWGYFVPARVDEAIPWLVPPL
                     HARFLGGMYLSGTAFMIAGIFSRRFDEVKTMVPMIAIWTGMLFIVSLFYLGEFDYSRP
                     QVWIWFGAYLAYPLIALWITWSHRTLNEPHDGTPLPAWAKNYLLVQGVLVTLLSLSLL
                     FAPDLMVSVWPWKITRLLAQIYSAPFLSYGLGSLMLSRRQTWKEARVVLIGTVVFAIF
                     VLLASIIHRELFSLSNASTWIWFGGFAIASLVLGGMTVRSLREKPE"
     gene            1550..1939
                     /locus_tag="PLG52_03060"
     CDS             1550..1939
                     /locus_tag="PLG52_03060"
                     /inference="COORDINATES: protein motif:HMM:NF025683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HNB40451.1"
                     /translation="MKSPTRLYTAVAGIFLLLQGVSTLTFRLVPALDAAFPQLLAVTQ
                     MVPTHSILHILTGILALIVLFHGGEKGSLWFSLGFGAFYTSLALYGFITHMPTMFHLQ
                     LFDHPFHLFLGVLGLAAGGFYFYSNRK"
     gene            1948..2769
                     /locus_tag="PLG52_03065"
     CDS             1948..2769
                     /locus_tag="PLG52_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40452.1"
                     /translation="MNTKSLEISFPLRAWLAVEVLFGIAAVLAVGLAPADTATNFAWP
                     IQPVVMAAVLGAFYITSAPLFLLPFLAKRWEMVRAMILPAALFSTVQLVATFLHWDKF
                     SVGTFPFYVWFASYLLPPPIFIAVYLWHQKQFGVESPIPAEDQIPAWLRTMLLVLGIA
                     LSLIAFIAFFFPSVIIPIFPWKLTPLTARSLSGWLVAVGTLMLSIRQGNSRVRSRLSS
                     PMLILILPSLLIQMGRYASEVNWASPTLWFGLLLFALTGFCGLTLAIGNWRESLT"
     gene            2771..3346
                     /locus_tag="PLG52_03070"
     CDS             2771..3346
                     /locus_tag="PLG52_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40453.1"
                     /translation="MHKYLKFFLLENLDTVRRIRVGSFWGVTLLITPWTWLSPFVFFG
                     LHFLLNLLNPRLPLDERLLQSIYFVIAVELTTPLHALGHIISGKLVHSAMDELLITAT
                     RDVNIYHGDQSQISGKVHLVRSLGGPILNILVGLFCILIAPSIPAGFWSGVNTSMIST
                     NLFFGLGGLLPVPSVDGEVIWREIINWMKKK"
     gene            complement(3374..4132)
                     /locus_tag="PLG52_03075"
     CDS             complement(3374..4132)
                     /locus_tag="PLG52_03075"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNB40454.1"
                     /translation="MRIIRYQTPEGTASYGWILEEKVGEVQGNMFGEYRRREARTPFK
                     ELKLLAPCEPSKIVCVGRNYVEHAKELGNEVPKIPLLFFKPPSSIIATGEHIVLPTQS
                     KQVEHEGELVVVMGKRAKNVTAEQAKEYIFGYTIGNDITARDLQKTDGQWTRAKGFDT
                     FCSFGPWIDTDFDPSDAVVTCRVNGQMRQMASTRDMVFNVGTLVAYISSVMTLEPGDL
                     IFTGTPAGVGELKNGDVVDVEIEGLGKLSNPVKA"
     gene            complement(4135..4620)
                     /gene="folK"
                     /locus_tag="PLG52_03080"
     CDS             complement(4135..4620)
                     /gene="folK"
                     /locus_tag="PLG52_03080"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNB40455.1"
                     /translation="MEHIVYLALGSNMGNRLANLKNAISNLTPQMDVKKKSPVYETPP
                     WGYTDQPAFLNQVVMVETYMEPENLLGHLKRLETVLGREPTFENGPRVIDVDILFYDD
                     LVLNSPPLVIPHPRLHHRGFVLVPLNDIAPELIHPILGKSISELLLDVSRLNINEYKG
                     K"
     gene            complement(4629..5468)
                     /locus_tag="PLG52_03085"
     CDS             complement(4629..5468)
                     /locus_tag="PLG52_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40456.1"
                     /translation="MSYKQRTIDFLEIEWATYIERFKRLPELDGVQRVRNQGYERFRD
                     MLAHVLAWWEDVMPVIVAIAENREFEKKKYDFDAFNAEAVAKYKDWDEAEFLAHFEKT
                     RQKAVADLKSMNEAAFEDRRIQGRINGIFIHHAREHLVALSKFLTLDTLEHEWGTYIA
                     RFDASEKKEEFLKKQSAARFGDLLAHNFAWWQEGVNAVQGALKDSSFVYKGPADTDAY
                     NAGIVAKYAGTSEEELRTIFEQKRLEMIELVRGLPDSAFQNPTIEEWLAADIVEHYDE
                     HAL"
     gene            complement(5504..5971)
                     /locus_tag="PLG52_03090"
     CDS             complement(5504..5971)
                     /locus_tag="PLG52_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNB40457.1"
                     /translation="METPPVSLALEKLNIPHQIFRHETPVDSFEKAASDRGQRPSQIV
                     RTILFRVSEEEFALVLVAGPGQISWKLLRKLLGKSRITMATEEEVISVTGYRPGTVGP
                     FGLLKPARIMVEAGLLKEEEVSIGSGMRDTAVILKTNDLMKALGNVEVVELFE"
     gene            complement(6025..6690)
                     /gene="nth"
                     /locus_tag="PLG52_03095"
     CDS             complement(6025..6690)
                     /gene="nth"
                     /locus_tag="PLG52_03095"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNB40458.1"
                     /translation="MTDLKQRALQIHETLLKVFGEPIWRNPLPAIDELVSTILSQNTN
                     DVNRDRAFEALRAKFPTWEAVRDAKEKDVVEAIKPAGLANQKGPRIQQVLKAITEERG
                     ELNLQFLADMPIEDARNWLTKFNGVGPKTAAIVLCFSLNRPAFPVDTHVYRVTGRIGL
                     RPEKMTVEQAHSHLESIFPPETYYAAHLNIIRLGREVCNARKPMCPQCPVKHLCDFKE
                     KTQ"
     gene            complement(6718..>7208)
                     /locus_tag="PLG52_03100"
     CDS             complement(6718..>7208)
                     /locus_tag="PLG52_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40459.1"
                     /translation="ESGELAKLKQAAVDFNWAPLQPLADAYASEQLSGCAEEAHKLMG
                     GLKTANESKVLYASWGMFKNLSFAALVQAGLMIQSENRAFSILEEHFGKTHPAWTRAF
                     RLAFGMDVETNVPAYQTRGKASLALYEETAKLFKGIINDNHREVIENTLQLINNFNRE
                     QK"
BASE COUNT         1544 a         1757 c         1826 g         2081 t
ORIGIN      
        1 ttgacggagg agaagttcga ttgggcgggg aaagcaaaca ggttatgacc agccttggaa
       61 gggcgagaca gttcgaggtc gagttgagag gcgtccaccc gcatttcggg cagcatgacc
      121 ggaatgtagg tcacctctgc gccacgggca tgtgcgaatt cgcgaatccc attcacagaa
      181 ttatggttgt cgaaggtcag gaggtaacgt ccgttcggga agggataact ctcgccgacg
      241 agtttgagcg cgccgctggc gttaggtgtg aagatggcga gatattcatc tggatcggcg
      301 ttgaagaact tgaggatgta ctcgcgtgtc ccttcgacca gatgtgtcgc ggcgatggaa
      361 gtggggttgg aggaatgcgg gttgccgaag acattttctt gtagaagctg ataatgcttc
      421 tttacctgtg actcggcata gatgccgccg ccggtgtaat cgaaataaac atgttcgccc
      481 gcatcgaggc gggaataatc gcttttgcga aggtcatcta ttttttcggt gtgtttgtag
      541 gtggggtatt tctgaaggaa ggtttcgaat tctgtagtca ttcgtggggc tcctgaaatc
      601 tatttaacca cacttggaag ttgggtggga gttcttcatt gggtttcttt ataaccaaag
      661 acaggctgtt aacctaacca aggttatatg tataatcagc caagcaaagt ttataaatac
      721 gcagtttcat cgttcttgag tcttttagtg aggtgtttat ggcattggta actcgtaaca
      781 tgaaagtgta ttttgccgca gtggggttgc tggcattgtg ggtgggggcg tggggatatt
      841 tcgtcccagc ccgggtggat gaagctattc cgtggttggt gccgcccttg cacgcgcgct
      901 ttttgggagg catgtatctt tcgggtacgg cgttcatgat cgctggcatc ttttcccgcc
      961 gttttgatga agtgaaaacc atggtgccga tgatcgccat ttggacgggc atgttgttca
     1021 tcgtctcgct tttttatttg ggcgagttcg attacagccg cccgcaggta tggatctggt
     1081 tcggagctta tttggcgtat ccattgatcg ctttgtggat tacatggtct caccgcactt
     1141 tgaacgaacc gcatgatggc acgccactcc cggcatgggc gaagaattat ctgcttgtgc
     1201 aaggtgtgtt ggttacctta ttgtctttgt cgcttctatt cgcgcccgat ctgatggtga
     1261 gcgtctggcc gtggaaaatt acccgtttgt tggcacaaat ctattccgca cctttcttgt
     1321 cgtacggctt gggaagtttg atgcttagtc gtcgccaaac atggaaagaa gcgcgcgtgg
     1381 ttttgatcgg aacggtagtc tttgccatct ttgtgctgtt ggcgtcgatc atccatcgtg
     1441 aacttttctc gctttccaac gcctctacat ggatttggtt tggtggcttt gcaattgcat
     1501 ctctcgtgct gggcggcatg accgtccgtt cgttgcggga gaaacctgaa tgaaaagccc
     1561 aactcgttta tatacagccg tggcagggat ttttctgtta ttgcaaggcg tatccacgtt
     1621 gacatttcgg cttgtccctg cgttggatgc ggcttttccg caattactgg ctgtgacgca
     1681 gatggttccg acccattcca ttcttcacat cctcaccggg attctggcat tgatcgtctt
     1741 gttccacggc ggtgaaaaag gaagcctgtg gttttcgctc ggctttggag cgttttatac
     1801 cagtttggca ttgtatgggt ttatcaccca tatgccgacc atgttccact tacagctttt
     1861 tgaccatccc ttccacttgt tcttgggcgt actgggcttg gcggctggcg ggttttattt
     1921 ttattccaat cgaaaatagg gattcatatg aacacaaaga gcttagaaat atcatttcca
     1981 ttacgtgcct ggttggcagt tgaagtgttg ttcgggatcg cggcggtgtt ggcggtaggt
     2041 cttgcccctg ccgatacggc tacgaatttt gcctggccca ttcagcctgt ggtgatggca
     2101 gcggttttag gcgcgttcta tatcacatcg gctcctttat tcttgttgcc ttttctcgcc
     2161 aaacgttggg agatggtccg cgccatgatc ctcccggcgg cgttgttcag cacggtgcaa
     2221 cttgttgcta cctttttaca ttgggataaa ttttccgtcg gtacttttcc gttctatgtt
     2281 tggtttgcca gctatctact tccacctcca attttcatcg cagtatatct ttggcatcaa
     2341 aagcaatttg gtgttgagtc gcccatccct gccgaagacc aaatccctgc ttggctaaga
     2401 acaatgttgt tagtgctggg cattgctctt tctctgattg cattcatcgc cttcttcttc
     2461 ccgagtgtca tcatccccat cttcccgtgg aagttaactc cgctgacggc tcgttcactg
     2521 agcggttggc ttgtggcagt aggcacgctg atgctttcta ttcgccaagg aaatagccgc
     2581 gtccgctcac gcctttcctc gcccatgttg attctcatcc tgccgtcttt gcttattcaa
     2641 atggggcgct atgccagtga agtcaattgg gcaagcccaa cgttgtggtt cggtttattg
     2701 cttttcgcgc tgacagggtt ttgtggttta actcttgcca tcggcaattg gcgcgagtcc
     2761 ctgacctaac atgcacaagt acctcaaatt ctttttgctc gaaaatcttg ataccgttcg
     2821 ccggattcga gttggttctt tctggggtgt gactcttctg atcaccccgt ggacgtggct
     2881 cagtcctttc gtctttttcg ggttgcattt cttgctcaac cttttgaacc cacggctccc
     2941 gctcgacgaa aggctacttc agtccattta ctttgtcatc gccgtcgagt tgactactcc
     3001 gcttcatgcc cttgggcata tcatcagcgg caaactcgtc cacagcgcca tggacgaatt
     3061 gctcatcact gccacgcgtg atgtcaacat ctatcacggc gaccaaagcc aaatttccgg
     3121 taaggttcat ctcgtccgtt cgttgggcgg acccattctc aatattctgg tgggtttgtt
     3181 ttgtatactc atcgctccat ccattcccgc tgggttttgg agcggcgtga acaccagcat
     3241 gatctccacc aatctattct tcgggttggg cggtctgttg cctgtccctt cagtagatgg
     3301 ggaggtgatt tggcgagaga ttatcaattg gatgaagaag aaataaaaaa gccccgcatt
     3361 tgcggggctt tttttatgct tttaccggat tacttaactt tcccagtcct tcgatttcta
     3421 catcgaccac atcaccattt tttagctcgc caaccccagc gggtgtgccg gtaaagatca
     3481 agtcaccggg ttcgagggtc atgactgagg aaatataggc aaccaaggta cccacgttga
     3541 acaccatatc tcgggtggat gccatttgcc gcatctgccc gttgacacga caggtgacga
     3601 ccgcatcaga cgggtcaaag tccgtgtcga tccacggacc gaaggaacaa aaggtatcga
     3661 agcctttggc gcgcgtccac tgaccgtctg ttttttgcag gtcacgagct gtgatatcgt
     3721 tgccaatggt atagccgaaa atgtattcct tcgcctgctc ggctgtgaca ttcttggcac
     3781 gtttgcccat caccaccacc aactcgcctt catgttctac ttgcttggat tgagttggaa
     3841 gaacaatgtg ttcaccggtt gcaatgatcg aagaaggtgg tttgaaaaag agtagcggaa
     3901 tcttcggcac ttcattgccc aattccttgg catgttctac atagttacgc cccacgcaca
     3961 cgatcttgct gggttcacat ggcgcgagaa gtttcagctc cttgaaggga gtccgcgctt
     4021 cacggcgacg atattcgccg aacatattcc cttgcacttc gccaactttt tcttcaagga
     4081 tccagccgta tgaggcagtc ccttccgggg tttgataacg gatgatgcgc atgtctactt
     4141 ccctttatat tcgttgatgt tcaatcggct cacatccaat aacaattcgc tgatggattt
     4201 tcctaaaatc gggtggatca gctcaggggc aatatcgttt agtggcacca gcacaaatcc
     4261 gcggtgatgc aaacgtgggt gaggaatgac cagtggagga gaattgagca cgaggtcgtc
     4321 atagaataag atgtcaacgt cgatcacgcg tggaccattc tcgaaggtag gttcccgccc
     4381 gaggacggtt tcgaggcgct ttaaatgtcc aagtaaattt tcaggctcca tgtatgtttc
     4441 caccatcacc acctgattca agaatgcagg ttggtctgta tatccccaag gcggagtctc
     4501 atataccggc gacttctttt tcacatccat ttgaggcgtg aggtttgaga tcgcgttctt
     4561 caaattcgcg agtcgattcc ccatgttact tccaagtgca agataaacga tatgttccat
     4621 gtggttcctt agagagcgtg ttcgtcgtag tgttccacaa tatcggcggc gagccattct
     4681 tcaatggtcg ggttttggaa ggcgctatca ggaagtccgc gcacgagttc gatcatttcc
     4741 aaacgttttt gctcaaagat ggttcgcaat tcttcttcac tcgttcctgc gtactttgcc
     4801 acaatgcctg cattgtaggc atcagtatct gccggacctt tatacacaaa gcttgaatct
     4861 ttcaaagcac cttgcacagc attcacgcct tcttgccacc atgcaaagtt atgtgccaac
     4921 aggtcgccaa aacgagctgc gctttgtttc ttcaaaaatt cttctttctt ctctgaagca
     4981 tcgaaccgcg caatataagt tccccattca tgttcaagcg tatcgagcgt gaggaatttg
     5041 ctcaatgcca ccagatgttc gcgggcatga tggatgaaga tgccgttgat gcgcccctga
     5101 atgcgacgat cttcaaacgc cgcctcattc atagacttta ggtctgctac ggctttctga
     5161 cgcgttttct caaagtgagc caaaaactcg gcttcatccc aatctttata ctttgccacg
     5221 gcttcggcat tgaaggcatc gaaatcatat ttcttctttt caaactcacg gttctctgca
     5281 attgccacga tcactggcat cacatcttcc caccaagcca agacgtgcgc caacatatcg
     5341 cggaagcgct catagccttg gttgcgaaca cgctgtacgc catccaattc aggcagacgt
     5401 ttaaagcgtt caatatacgt tgcccattca atttctaaaa agtcgatcgt gcgttgctta
     5461 tagctcattg atactctcca gatcataaga tgctagttag attttactca aacagttcta
     5521 ccacttccac atttcctaac gctttcatta agtcattcgt tttgaggatg acggctgtgt
     5581 cgcgcatgcc cgacccaatg gatacttcct cttccttcaa aagacccgct tccaccatga
     5641 tgcgggcggg cttcaacaac ccaaacggac ccactgtccc tgggcgatag ccggtgacgg
     5701 atatcacttc ttcttcggtt gccatggtaa tgcgtgattt gccgagtaat tttcgcagca
     5761 acttccacga aatttgaccc ggtcctgcta cgaggacgag cgcaaattcc tcttcactca
     5821 cacggaagag gatcgttcgc acaatttgcg aagggcgctg accacggtca ctggcggctt
     5881 tctcgaacga atccacaggt gtttcatgcc tgaagatttg atgagggata ttcagttttt
     5941 ctaaagcaag gctgacggga ggtgtttcca tggcatgcat tctaactgaa aataaaaact
     6001 gcgccataac agcgcagtgt agtcctattg agttttctct ttgaaatcac atagatgttt
     6061 gaccgggcat tgcgggcaca tcggcttgcg cgcattgcag acctcccgtc ccaaacggat
     6121 gatgttcagg tgagcggcgt agtaggtctc tggcgggaag atggattcaa gatgggagtg
     6181 tgcttgctcc acggtcatct tttcggggcg cagtccaatg cgacctgtca cgcgatagac
     6241 atgggtatcc accgggaagg cggggcgatt gagagaaaag cataaaacga tggcagcagt
     6301 tttgggaccc acgccgttga atttggtcag ccagttgcgg gcatcttcaa tgggcatatc
     6361 cgcgagaaat tgcaggttga gttcaccacg ttcttcggta atggctttca acacctgctg
     6421 tatgcgcgga cctttttgat tcgccagccc ggcaggtttg atggcttcga ccacatcttt
     6481 ttctttggca tcgcgcacgg cttcccatgt ggggaacttg gcacgcaaag cctcaaaagc
     6541 acggtcgcgg ttcacatcat tggtattctg cgaaaggatg gtggagacga gttcgtcaat
     6601 agccggcaga ggattgcgcc aaatgggctc gccaaagacc tttagcagag tttcatggat
     6661 ttgtagagca cgttgtttga ggtctgtcat gaggtttatt aaaagaaaga agctgtttta
     6721 tttttgttcg cggttgaaat tattaatcaa ttgtagcgta ttctcaatga cttcacgatg
     6781 gttgtcgttg atgatgcctt tgaataattt tgctgtttct tcgtagagtg ccaaggatgc
     6841 tttgccgcgc gtttgatacg ccgggacatt tgtttctaca tccataccga aggcgaggcg
     6901 aaaggcgcgc gtccatgcag ggtgagtctt tccaaaatgt tcttcaagga tggaaaaggc
     6961 gcggttctcg ctttggatca tcaagccggc ttgcaccaac gccgcaaagg ataaattttt
     7021 gaacatgccc cacgaagcat acaaaacttt tgattcattg gcagttttca acccacccat
     7081 gagcttgtgc gcttcttcgg cacagccaga aagttgttcg ctggcatagg cgtctgcgag
     7141 aggttgcaga ggtgcccagt tgaaatccac ggcggcttgt ttgagtttgg caagttcgcc
     7201 gctttcat
//
LOCUS       DAOQNJ010000062         7158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5581_length_7158_cov_10.049979, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000062 DAOQNJ010000000
VERSION     DAOQNJ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5581_length_7158_cov_10.049979"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PLG52_03105"
     CDS             complement(<1..520)
                     /locus_tag="PLG52_03105"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNB40460.1"
                     /translation="MSKENEAVILSAARTPIGKFQGALSGIPAPKLGAIAVEEAVKRA
                     GIDPSEVEEVFMGNVVQAGNGQAPARQAAIYGGIPATVSATAINKVCGSGLKAAMMSA
                     QAIRAGDAELFIAGGFESMSRAPYLVSGRMGELKFGNSQMTDALLNDGLWDPFENWGM
                     GNAAEFIADEYEV"
     gene            688..3567
                     /locus_tag="PLG52_03110"
     CDS             688..3567
                     /locus_tag="PLG52_03110"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNB40461.1"
                     /translation="MSDVFISYSRRDKVFTQKLYEALRDSGREIWADWDSIPAASDWF
                     AEIKDGVEKANTIVFVLSPEWLKSNECRKEYDHAVAMGKRLLPILYQVVDPKEVPSEL
                     AKINWVYMRDTDDFGQAFQTLSAAMDTDLEWVKTHTRIQIRAIEWDKKSRDHSFLLRG
                     NDLTDGEQFIAQGAQKSPEPTVLHGEYVLASRKDATRRQRMTLAGVTVALVVSVALGI
                     VAFFQRQAAVAARAEAVVERDRAVAAEKIAEDERDRADINAEVAYSRELAAASLNSLN
                     HDPELSLLLGLQSVAITDKTGMPIQPMSEDALRRSVQTSRIRKTWLAHSGSVLDIAIS
                     PDGTKIASASDDLTVKIWDLSGKLLLTIPDFSEEVDSVVFSPDGSQILTSSGQEAVAW
                     DAVTGEKLVEYKGHVSYVVQAVYSPDGKFIATASSDATAIMWDALSGESLVVLGGHET
                     ELSDIAYSPDGEMIASTDYNGAMYVWSTSSKSLLYMLSEGEGSVILDVEFSPDGKYIL
                     ISDNYFQTIVLVAQTGKVISAQNVHTDFVTNAVVSPDGAFMASASTDNTVVVSSITAS
                     AVLFGQSISADPDYIDISKVMFRFTDSTDINTMIYTPDGKYLITGNDEGQIKIWDANP
                     ISDYEVSGFRPHTARIDGMEFSPDGEHYVTVARDGKAFIWSLTDQSSIELVGHQNVIQ
                     DVAYSSDGMQIITGGFDGHAIIWDAKTGDKLITLSKDDQLVTGVNFSPDGKLAVGSLG
                     DNTVVVWDAVSGDEVRVLQSKDMYAYINELAFSPDGKTLAVSSDAGFVVLWDVATWNQ
                     IDVLAGHTDWVKGLDYSSDGTHMVTASYDTTAIIWDVAARTPVLKLEGHSQGVNDVTY
                     SPDDTLVATASVDGTAKLWNANTGELLVTYQTSADQILSVAFSADGKYLATGSETGVV
                     RIYYVYAEDALELAKSRITRTFTEEECLKDLHEEACPANLPQPQQ"
     gene            3632..4045
                     /locus_tag="PLG52_03115"
     CDS             3632..4045
                     /locus_tag="PLG52_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNB40462.1"
                     /translation="MTEQSLYELLGGEEGVRTLVDRFYDLMDSSPEAKDVRALHAKSL
                     KQAREKLFMFLSGWSGGPPLYIEKFGHPRLRMRHMPFVIGTRERDQWLWCMNRAVDAG
                     NYHSAVVEHLKQRFSEIADFMRNQDETEQGPLLQL"
     gene            complement(4042..4716)
                     /locus_tag="PLG52_03120"
     CDS             complement(4042..4716)
                     /locus_tag="PLG52_03120"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNB40463.1"
                     /translation="MHPYVRSLKILFEANANPVNAAPMKKYMRDQFEYLGIKSPQVKS
                     LFSGYIKQNSLPPVEDLSTIVRELWNLPQREFQYLAINLLDKMEKKLPEDFIVTLEYL
                     ITHKPWWDTVDLIAGHSVGMMFKRYPKVKAQYLKKWRKSENIWLRRTTLLFQLGYKKE
                     TDFGLMCELIQENLGSDEFFINKAIGWALRQYAWTNPSAVKKFVKATKELSPLSQKEA
                     LKNIGA"
     gene            complement(4728..6332)
                     /locus_tag="PLG52_03125"
     CDS             complement(4728..6332)
                     /locus_tag="PLG52_03125"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNB40464.1"
                     /translation="MKRITSLIWIACLLCACAGSATVETTIENTATVLPSATPTPIFT
                     PTPQPHSQPISYGPDRENFPSNVNPLSGLQVEDPSLLKTPAIALSISNFPVAARPQAG
                     LSFAPWVFEFYITEGATRFLSIFHGAFPKPEAPIVGNCEVRREPIQGNVIEIGNRIWL
                     DENQNGIQEDYEKGVGGVCINLLDATGKSVQKTTSDSNGFYGFVTEPGNYIVEVTIPE
                     WLKFTKPNVGDEEHDSDADPVSGKIEADLISTPLDLDVGLVPSEKANPPVKQSAQLPP
                     AEVGPIRSGRLLYVDVHSMFLNSCFVFASASEEILPLLPQCYLVPHDEASGGAMLSIE
                     KMKSLAEENKGTRPVDFNYASNVFAETPPEGGETALRLNSYVALLNQSAWVYDPASSS
                     YWRYVDDTTQANAGVLHPEIDRLTGRQLQFNNIIVLEVDQEVYEYQDRPIRTLLDYHL
                     ELGQGGYAYLFRDGRKYDIRWTTKARAYEKQTGQARPLYFVDANGDPVSLKPGNTWFI
                     VATPYSYFTPEDGVWNFRFVPPEGVK"
     gene            complement(6350..>7158)
                     /locus_tag="PLG52_03130"
     CDS             complement(6350..>7158)
                     /locus_tag="PLG52_03130"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HNB40465.1"
                     /translation="AGLVLTSQPSTTSTLPAAKVGPVRSGRLIYADIAAFFPDSCLIY
                     AFASPEVLELLPQCYFVQHDLEGGGYMLDIDELKRLAQESKQYDVDYASNAFDENIPE
                     GGEPASRLDVYFAYLNQSAWVYDAAAESYWRYVDNADYDNAGVLHPEIDRLTGRQLQF
                     ENVVVLFTEHDVVSPTNLDIHLEEDKEGYALLFRDGRMYDMFWSTDLSDAERESGRHK
                     PIHFINPDTKQLMPLKPGRTWIIVVTPETAVTPQADSKWYLEFYQPAGAQ"
BASE COUNT         1833 a         1565 c         1790 g         1970 t
ORIGIN      
        1 tcacttcata ttcatctgcg ataaattctg cggcgttgcc catgccccaa ttttcaaacg
       61 gatcccacag accgtcgttg agcagggcat cggtcatttg ggagttgccg aatttcaact
      121 cgcccatgcg cccgctgacg agatacggag cgcgactcat cgactcaaac ccaccggcga
      181 tgaagagctc cgcatcaccc gcccgaatgg cttgggcaga catcatcgcc gctttcaagc
      241 ccgagccgca gaccttgtta atggcagtcg cactgacggt tgcagggatg ccgccgtaaa
      301 ttgccgcttg tcgtgccggt gcctgaccat tgccagcctg caccacattg cccatgaaaa
      361 cttcttccac ttcgctgggg tcgatgcctg cgcgtttaac cgcttcttcc acggcaattg
      421 caccgagttt cggcgctgga ataccgctca acgcaccttg gaattttccg atcggtgtgc
      481 gggctgcgct caatataacc gcttcatttt ctttggacat cgtacctcct gtggggtgat
      541 attcgtatta tacgcgagag ccgccatagg gcagaaattc tgttaagttc tttcattctc
      601 tgctttcaaa cttgtaggtc tttccataac aaagcctact atataatcga cacatttctt
      661 atggatatat aggatcagga gagcgacatg tcagatgtat ttatttctta ttcaaggcgc
      721 gacaaagtat tcactcaaaa actatatgaa gcactaagag actctggtcg tgagatttgg
      781 gcggactggg actcgatccc agcggcatcg gattggtttg ctgagatcaa agatggggtg
      841 gaaaaagcaa acacgattgt ttttgtgtta agcccggaat ggttgaaatc gaacgaatgc
      901 cgcaaagaat atgatcatgc cgtggcaatg gggaaacgat tattgcctat cctatatcaa
      961 gtagtagacc caaaggaagt tccgtctgag ctggcgaaga ttaactgggt ctacatgcgt
     1021 gatacagatg attttggtca ggcattccaa accctttctg ccgccatgga tacagacttg
     1081 gaatgggtaa aaacccatac tcgtattcaa atccgtgcga ttgaatggga caagaaaagt
     1141 cgtgaccatt cattcttatt gcgtggaaat gacctaacgg atggagagca atttattgct
     1201 caaggggcac agaaatcacc ggaacctact gttctccacg gtgaatatgt gcttgccagc
     1261 cgaaaggacg ccactcgccg tcagcggatg actttggcag gtgtaacggt tgcactggtt
     1321 gtttcggttg ctttgggcat tgtggcattt ttccagcggc aggcggctgt tgctgcgcgt
     1381 gcagaagctg tggtggaaag agatcgtgct gtggctgccg aaaagattgc ggaagatgaa
     1441 cgagatcgtg cggacattaa tgcagaagtt gcatattctc gtgagctagc cgctgcttct
     1501 ttgaattcat tgaaccatga ccctgaactc agtctgttgt taggtttgca atctgttgct
     1561 attacagata aaacggggat gccaattcaa cctatgagtg aagatgccct gagacgttct
     1621 gtgcaaacat cacggattcg aaaaacctgg ttggcacatt caggttcagt tctcgatatt
     1681 gcaattagcc ctgatggaac taagattgca tctgccagtg atgatctaac agttaagatc
     1741 tgggacttgt ctggtaaatt attattgacc atccctgatt tttcagaaga agtcgatagc
     1801 gttgtgttta gtccagatgg ttcacagatt ctgacctcaa gcggacagga agcagttgca
     1861 tgggatgctg tcacaggcga aaaattggtc gaatataaag gtcatgtatc ctatgttgta
     1921 caagcggtct atagcccaga cgggaaattt attgccaccg ctagttcaga tgcaactgca
     1981 attatgtggg atgctttatc tggagaaagc ttggtagtgc tcggtgggca tgaaacagaa
     2041 ttgtctgata tcgcatatag ccctgatggc gaaatgattg cctcgacaga ctataacgga
     2101 gctatgtatg tttggagcac cagttcgaag agtttattgt atatgctttc agaaggggaa
     2161 ggttctgtta tccttgatgt agaatttagc ccagatggta agtacattct tatttctgat
     2221 aattattttc aaacgattgt tcttgttgcc caaactggca aggtaatctc agcgcagaat
     2281 gttcataccg actttgtgac caatgctgtg gttagtccgg atggagcatt tatggcaagt
     2341 gcctctacag acaataccgt ggttgtaagc tcaataactg caagcgcagt attgtttgga
     2401 caaagtattt ctgccgaccc agattatata gatattagta aggtcatgtt caggtttaca
     2461 gattctacag atattaatac tatgatctat acccctgatg gaaagtacct cattacagga
     2521 aatgatgaag ggcaaataaa aatatgggat gcaaacccaa taagtgatta tgaggtgagc
     2581 ggattccgtc cacatacagc acgtattgat ggcatggagt ttagcccaga tggtgaacat
     2641 tatgtcactg tagctcgaga tgggaaggcg tttatctggt cattgacgga tcaatcgtcc
     2701 attgaattgg tcggacacca gaacgtcatt caagatgtag cctatagctc tgacggaatg
     2761 cagataatca ctggcggttt tgatggtcat gccattattt gggatgctaa aacaggcgat
     2821 aaattaataa cactttctaa ggatgatcag ttggttactg gggttaattt tagccctgat
     2881 ggtaaattgg ctgtaggctc attgggtgat aatacagtag tggtttggga tgctgtatct
     2941 ggtgacgaag tacgtgtttt gcagtcgaaa gatatgtatg catacatcaa cgaattggct
     3001 ttcagcccag acggtaaaac tcttgctgta agtagcgatg ccggatttgt tgttctatgg
     3061 gatgttgcta catggaatca aattgatgta ttggctgggc ataccgattg ggtcaagggc
     3121 ttggattaca gttctgatgg tacgcatatg gttacggcaa gttatgacac cacggcgatt
     3181 atttgggatg ttgcagcgcg gacccctgtt ttgaaattgg agggacattc ccaaggagtg
     3241 aatgatgtga catacagccc agatgatacg ctggtggcta ctgcaagtgt agatggaacc
     3301 gccaagcttt ggaacgcaaa tactggggag ttattggtca catatcaaac ctcggcggac
     3361 caaatcttaa gtgtggcatt tagcgctgat ggaaaatatt tggcaactgg cagcgagacg
     3421 ggcgtggtgc gtatctacta tgtatatgct gaagatgccc ttgaactcgc caaaagcagg
     3481 atcacgcgca cgttcacaga agaagaatgt ttgaaagatt tgcacgaaga agcttgcccg
     3541 gcgaatctcc ctcagccaca acagtaacta aagtaaaccg ccttctagta gatagaaggc
     3601 ggtttttatt tctttcgatt acaatgccgc tatgactgaa caatcccttt atgaattact
     3661 cggcggtgaa gagggtgtgc gcaccttggt tgaccgcttc tatgacctca tggattcttc
     3721 gccggaagca aaagatgtac gtgctttgca tgccaaaagc ttgaagcaag ctcgcgagaa
     3781 gttatttatg tttctctctg gctggtcagg cggacctccg ctatatatcg agaaatttgg
     3841 gcatcctcgt ttacggatgc ggcacatgcc gtttgtcatt ggtacgcggg aacgtgacca
     3901 gtggttatgg tgtatgaatc gcgccgtgga cgcagggaat tatcactcag ctgtggttga
     3961 gcatctcaaa cagcgattct ccgaaattgc agacttcatg cgcaatcagg atgaaactga
     4021 acaaggtccc cttttgcagc tttaagcgcc aatatttttc agcgcttctt tttggctaag
     4081 tggacttaat tcctttgtgg ctttcacgaa ttttttcaca gcgctgggat tggtccacgc
     4141 atattggcgt aatgcccaac cgatggcttt gttgatgaag aactcatcag agccaaggtt
     4201 ctcttgaatt agctcgcaca ttagtccaaa gtctgtttcc tttttatagc ccagttgaaa
     4261 caacagagtg gtgcgtctga gccagatgtt ctcagatttg cgccactttt tcaaatactg
     4321 tgccttgacc tttgggtagc gcttaaacat catgccaacg ctgtgccctg caatgaggtc
     4381 aaccgtgtcc caccagggtt tgtgagtgat aaggtattca agcgtaacaa tgaaatcttc
     4441 aggtagtttc ttttccattt tatcgagcaa gttaatggca aggtactgaa actcgcgttg
     4501 agggagattc cataattcgc ggacgatggt gcttaagtct tctacaggcg gcaaactgtt
     4561 ttgcttgatg tatccagaaa acagactctt cacttgcggg ctttttattc ccaggtactc
     4621 aaattggtcg cgcatgtatt tcttcatcgg cgcggcgttg acggggttgg cattggcttc
     4681 aaaaagtatt ttcaaggaac ggacataagg gtgcatagtt tcctctttta tttcacacct
     4741 tcaggcggca caaaacggaa attccaaacg ccatcttcgg gcgtaaagta cgaatagggc
     4801 gtggcaacga tgaaccaggt attgcccggc tttaaggaga ctgggtctcc attcgcatct
     4861 acaaaatata acgggcgggc ttgcccggtt tgtttttcat aagcacgggc tttggttgtc
     4921 cagcgaatat catattttct gccatctcga aagagataag catacccacc ttgcccaagt
     4981 tcgaggtgat agtctagcaa ggtgcggatg ggtctatcct gatactcata gacttcctgg
     5041 tcaacttcca atacgatgat gttattgaat tgtagctgcc tgcctgtcag gcggtctatt
     5101 tctggatgta agactccggc atttgcttgt gtcgtgtcat ctacataacg ccaatacgaa
     5161 ctggaggcgg ggtcatacac ccatgcagat tgattgagta aggctacata agaatttaaa
     5221 cgcagcgctg tctcaccacc ttcgggtggc gtctctgcga aaacattgct ggcgtaattg
     5281 aaatcaaccg gtcgtgtgcc tttattctct tctgccagcg atttcatctt ttcaatgctt
     5341 agcattgcac cgccactggc ttcgtcatgt ggcaccaaat agcattgtgg caacaatggc
     5401 agaatttctt ccgatgccga tgcaaagaca aaacatgaat ttaagaacat gctgtgaaca
     5461 tccacgtata acaagcgtcc cgagcggatg ggtcccacct cagcgggggg gagttgggct
     5521 gattgcttca cgggtggatt cgctttttcg gacggaacca agcccacgtc taagtcaaga
     5581 ggcgtggaga taagatccgc ttcgatcttc cccgaaacag ggtccgcgtc actgtcatgt
     5641 tcctcatctc caacatttgg cttcgtaaat ttcaaccact ctggaatggt cacttcaacg
     5701 atatagtttc ccggctctgt gacgaagccg taaaaaccgt ttgagtcgct ggttgttttc
     5761 tgaactgatt tcccggtcgc atccaataaa ttgatacaga ctccccctac acctttttcg
     5821 taatcttctt gaatgccgtt ttggttttca tccaaccaga tacgatttcc gatctcaata
     5881 acattgcctt gaatgggctc acgcctcact tcgcaattgc caacgatggg agcttctggc
     5941 ttaggaaatg ctccatgaaa aatggagagg aaccgggttg cgccttcggt gatgtaaaac
     6001 tcaaagaccc atggcgcaaa cgataaccct gcctgtgggc gagccgctac gggaaaattt
     6061 gaaatggaga gtgctatggc tggagttttt aataagcttg gatcttccac ttgcaacccc
     6121 gaaagcggat ttacattcga cggaaaattt tcgcggtcgg gaccatatga gataggctgg
     6181 ctgtgaggtt gaggtgtagg tgtgaagata ggcgtgggcg ttgcagacgg taagactgta
     6241 gcggtatttt cgatagttgt ttcaacggtt gcgctgccag cacacgcgca gagtaaacat
     6301 gctatccaga tcaacgatgt aattcttttc aagatgaaaa ctccaaaact tattgtgccc
     6361 cagccggttg ataaaattcc aaataccact tgctatcggc ttgcggtgtt actgccgttt
     6421 caggggtgac gacgatgatc catgtgcgac cgggtttgag cggcatgagt tgtttggtat
     6481 ctgggttgat gaagtgaata ggtttatgtc ttccgctttc acgttctgcg tcgctcaggt
     6541 ctgtgctcca aaacatatca tacatgcgtc catcacggaa taataaggca tagccttctt
     6601 tatcttcttc aaggtgaatg tcgaggttgg tgggagagac aacatcatgc tctgtgaata
     6661 acaccacaac attttcaaat tgaagttggc gtcctgtcaa tcggtcaatt tctgggtgga
     6721 gaacaccggc gttgtcataa tcggcattgt ccacatagcg ccaataactt tcagccgccg
     6781 catcatacac ccatgcagat tgattgagat aggcaaaata cacatccaag cgtgatgcag
     6841 gctcaccacc ttcgggaata ttttcatcga aggcattgct ggcgtaatcc acgtcatatt
     6901 gtttactttc ttgtgccaag cgtttgagtt cgtcaatatc caacatgtag ccgccgcctt
     6961 ccaaatcatg ttgaacaaaa taacattgcg gcaaaagttc caatacttca ggcgaggcga
     7021 aggcatagat gagacaagaa tctgggaaga atgcggcgat gtctgcgtag atcaaacgcc
     7081 ccgaacgcac gggtcctact ttggcagcag gaagtgtgga tgtagtagaa ggctgagagg
     7141 tgaggaccag gcctgcgt
//
LOCUS       DAOQNJ010000063         7122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5643_length_7122_cov_8.380359, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000063 DAOQNJ010000000
VERSION     DAOQNJ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5643_length_7122_cov_8.380359"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..876
                     /locus_tag="PLG52_03135"
     CDS             73..876
                     /locus_tag="PLG52_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40466.1"
                     /translation="MTILYKQALDLLDARSLVTSLDHDLTRLIRRELSASLGGSDDIA
                     DRVLKNILLRWNCALSAADDEVTARQKNLIVVTLPLLLHKYGIQDPSLNSKAYQAIEA
                     LNREVILSDSFYRKSSAIKELLSSPVALRSRKPARPADLTFWRTGDLVAYQIAGKFYA
                     LYIHGIVGANEAPIVEFMDMETSYRPVRDDVLGKKARGIPFKDGVLHVQHYLVYGMRD
                     MPDPANQFHLLGNMPQYKPDQTHLAPPIGMGIVTDVFRLADEVREKLFP"
     gene            887..1513
                     /locus_tag="PLG52_03140"
     CDS             887..1513
                     /locus_tag="PLG52_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40467.1"
                     /translation="MMTENFIFPFELQQSRPFHFVEETSLAAARIGGTMPDGISVPLR
                     DDARYFGTFPLYDGNSRLFFSIFINGSFASFIHALNAGFQTDDRVVVIVHEKKPRSTD
                     TKYASGLSPHSIQVHDIASDLIQNDIGETVIRERHKFGGRPYSIQEPILPGSEALFAQ
                     GYRQVLQIDFPNPTDGAISGSWPFGDGIFNLFWKAPFEKEPYFWCLQG"
     gene            1623..2243
                     /locus_tag="PLG52_03145"
     CDS             1623..2243
                     /locus_tag="PLG52_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNB40468.1"
                     /translation="MINPEEAVIINSLVRQALTSMQEVMGDNGLNAVLKSCGLERFVG
                     NFPPNNLEPAIKTSQYAQLNQAIEDFYGRGGKGMLQRIGRASFQYGVREQVALLGVAG
                     VALKLMPEKQRIKFILNGMADALKKSNAQVKAWVDESGERLAYVDATCGICNSRHSDH
                     PICHLYIGSVSEAVKWATGKDYEVIETHCTAMGDEYCRFEVGNVKE"
     gene            2442..3398
                     /locus_tag="PLG52_03150"
     CDS             2442..3398
                     /locus_tag="PLG52_03150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB40469.1"
                     /translation="MKNTELALVALINILVFGGNFYLLQKRKYLLAMHIGITAITIAS
                     LVSNFYLGWESYFSLYLIASTIIIINSVTLSLRRKLIEVTALMTLFVASFIHMKTFTP
                     LYDLNPVLVERIGLFNSGAVLFSVLFMQFHNFIENENLKKKLQEMSEIDPLTGAYNRR
                     FFNEYLDIELKRLESPSRYAPAKKTNFGIAIIDIDDFKMINDMYGHLVGDMVIAEVAG
                     IIKSAIFKRDIFCRYGGEEFLVLFTNTSKSGVKIAIEKIRTAIQNHVFMTHGERLNIE
                     VTVSVGFAGFSEEANVYKLLDLADKRLYKVKGAGKNGAILGE"
     gene            complement(3461..4894)
                     /locus_tag="PLG52_03155"
     CDS             complement(3461..4894)
                     /locus_tag="PLG52_03155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB40470.1"
                     /translation="MTNESGKKPAMHKKHVARLERELQQTRLILYTFFGILGVVAVLL
                     IYGWLDVNYLQLNRAVATVNDNKIVLKDFEARTRLRRQQLIDNYNQYTQYQQYFGMDM
                     SSQLNSIETQLNTPELVGTSVLDAMIDEEIIRLEAEKRGITVSEEEIDKKMEEGFNFY
                     PNGTPTPSLTPTPADLPTDPAELLQFITATPDVTATPLFTATAEFTATVESQPTAEGD
                     ATATLAPTNTAEPTVTPTATLGPTSTPEPTATPFTREAYEVLLGKTKDNITKYGFDET
                     YIREFFRNQILREKLLEVIAADVKPFEEQVRARHILVADEQTAKDLIARLQAGEDFAD
                     LARQYSSDSSSEQGGDLGWFGKGQMVAEFETAAFALQNPGDFTTEPVASQFGYHIIQL
                     MAKQERPLSASELQNAKETALQDWLTKARDTDYTVETFDFWKQRVPSTPNFITAATES
                     AAAQKTAQAESVATLDAKNSAAATPTP"
     gene            complement(5000..5293)
                     /gene="rpsR"
                     /locus_tag="PLG52_03160"
     CDS             complement(5000..5293)
                     /gene="rpsR"
                     /locus_tag="PLG52_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNB40471.1"
                     /translation="MSDERPERPERSGGEGGGERGDRKFFAKPKFCQFCADKTLTIDY
                     KKVDLLRKYVTEEGSLRPRRQTGACAKHQRLVAAAVKQARHVALLPFANRGVD"
     gene            complement(5344..5751)
                     /locus_tag="PLG52_03165"
     CDS             complement(5344..5751)
                     /locus_tag="PLG52_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB40472.1"
                     /translation="MSRGLNKVQIIGHLGKDPEMRYTPSGKPVTTFTVAVSRTWNSAD
                     GERHNETEWFNVVAWGTLAEICKQYLVKGQQVYVEGRLQTRRWDDKEGIKHTSVEIVA
                     NEMMMLGDRREANNQPDASPAESNGAADEEFPF"
     gene            complement(5788..6072)
                     /gene="rpsF"
                     /locus_tag="PLG52_03170"
     CDS             complement(5788..6072)
                     /gene="rpsF"
                     /locus_tag="PLG52_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNB40473.1"
                     /translation="MRNYELMCIIQPDLDETAMNGVLDKVKGWITEAGGSIDKAEVWG
                     RRRMAYSINKHREGQYVLLNVSMSPAATSDLERNLRYQESIMRHMLSVVG"
     gene            complement(6264..6935)
                     /locus_tag="PLG52_03175"
     CDS             complement(6264..6935)
                     /locus_tag="PLG52_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNB40474.1"
                     /translation="MRLSVLRVINIMRISVYVDGFNFYHGLTENTKYRWCDLLKLTKQ
                     FLPNGEYGKIKLFAAHSKEYYDNLGKPERQNRYLQALKTIPGFEFIPSKFSQFKKTLP
                     LRDSPKDKPEFVHVRGYKEKGADVNLASHLISDGIKNLYDIAVVFTNDSDLVEPIRVV
                     RYDLNKKVYILLTCKNRNAWVSKELITVAKYHKLVEEDHLRVSQLPDVVTRPGKPALK
                     KPLSW"
BASE COUNT         1705 a         1542 c         1842 g         2033 t
ORIGIN      
        1 cctaatcaat aagatggagt taggaaatta gtctgattga ccattgcatg aaattgaaga
       61 aaacttgggg gtatgacaat actatacaaa caagccttgg atttgctcga cgcgcgctca
      121 ctggtgacgt cgctcgacca tgacctgacg cgcttgatcc gcagggagtt atccgcttcg
      181 cttggcggtt ctgatgatat agcggatcgg gttcttaaga atattctgct tcggtggaat
      241 tgtgctttat ctgccgctga tgatgaagtg accgcgaggc aaaagaattt gatcgtggtc
      301 acgttgcccc tcctgctcca caaatatgga attcaagacc cttctcttaa ttcaaaagct
      361 tatcaggcta ttgaggcgct taaccgggaa gtaattctca gtgattcatt ctatagaaag
      421 tcttcggcga tcaaagaact cctatcctct ccggttgctc ttcgctcacg aaaacccgcc
      481 cgcccggcag atctcacttt ttggaggaca ggagatttgg tggcgtatca aatagcgggg
      541 aaattttatg ccttgtatat ccatggcatt gtaggcgcga atgaagcgcc tatcgtagag
      601 ttcatggata tggagacgtc ttatcgccct gtgagggacg acgtcttggg gaaaaaggcg
      661 cgcggtatcc catttaaaga tggagttctt catgtgcagc actatcttgt ctatggcatg
      721 cgtgacatgc ccgacccggc gaaccaattc catctgctcg ggaatatgcc ccaatacaaa
      781 ccagaccaaa ctcatctcgc tccacccatc ggcatgggca tagtgacaga tgttttcagg
      841 cttgcggatg aagtccgcga gaaattattt ccttgaaaag gaacgtatga tgaccgaaaa
      901 ttttattttc ccattcgaac ttcagcaatc ccgtccgttc cattttgtcg aagagacttc
      961 tctcgcagct gcaagaattg gcggtaccat gccggacgga atatccgtcc ctctccgtga
     1021 cgatgcccgc tattttggaa cctttccgct ttatgatgga aattcccgcc tcttcttttc
     1081 catttttatt aatggctctt tcgcaagctt tatccacgcc ttgaatgccg gttttcaaac
     1141 tgacgataga gttgttgtta tcgtccatga gaagaaacca cgcagtactg atacaaaata
     1201 tgcatctggg cttagtccac attccattca agtccatgat attgcttcag acctcattca
     1261 gaatgatatt ggtgaaacag tgattcgtga gcggcacaaa tttggcggac gtccatacag
     1321 tattcaagaa cctatcctgc caggaagtga ggcgttgttc gcacaaggct atcggcaagt
     1381 tttacagatc gactttccaa accctaccga cggagcgatc tctggctcct ggccctttgg
     1441 tgatgggatt tttaatttgt tttggaaagc tccattcgaa aaggagccct atttttggtg
     1501 tttgcaggga taagaacgtc tttttgaaaa gtttaagatt ggtactataa ggtcattaca
     1561 catactttca atagtgagta tgctaaagaa agaatctttg atccataacg ataggagccc
     1621 atatgataaa cccagaagaa gccgtcatta tcaactcgct cgtgcggcag gcgttgacct
     1681 ccatgcagga ggtgatgggg gataacggtt tgaacgccgt cttgaaatct tgcggattgg
     1741 aacgttttgt agggaatttc cctccgaaca accttgagcc tgcgatcaag acctcacaat
     1801 atgctcagtt gaatcaagcc attgaagatt tttacgggcg cggcggcaaa ggcatgttgc
     1861 aacgcattgg cagggcatcc tttcaatatg gagtacgaga acaggtggct ttgttgggtg
     1921 ttgccggtgt ggcactcaag ttaatgcccg aaaaacagcg cattaagttc attctaaatg
     1981 gcatggcaga tgcgctcaag aagagcaatg cacaggtcaa agcttgggta gatgaaagcg
     2041 gtgaacggct ggcatacgtc gatgctactt gcggaatctg taactcgcgc catagtgacc
     2101 atcccatttg ccatttgtat atcgggtccg tttctgaggc ggtgaaatgg gcaaccggca
     2161 aggattatga agttatcgaa acacactgta ctgccatggg cgatgaatat tgtcgcttcg
     2221 aggtaggaaa tgtgaaggaa taatttgaaa attgtaagag ttttctccgc ctcttggttt
     2281 taggggcggt tttttatgtg gggggatata atcgaacaaa gtttgagtat gatgatatga
     2341 atgtgaatga tttagatgag aaaagaaaat attctctttt aagaatattc ttttctctaa
     2401 gcttggtaat caatgctttt tttgttgttc ttcaacttta tttgaaaaac accgagttgg
     2461 ctttggttgc cttaatcaat attcttgtgt ttgggggtaa tttttacctt ctccaaaaga
     2521 gaaagtattt gcttgccatg catattggca ttacggcaat cacaatcgct tctttagttt
     2581 ccaattttta tttgggatgg gaaagttatt tcagcctgta tctaattgct tccaccatca
     2641 ttattatcaa ttcggtaacg ctctctttga ggcggaaatt gattgaagtg accgctctta
     2701 tgacgctatt cgttgccagt tttattcata tgaagacgtt tacgcctctc tatgacctga
     2761 acccggttct cgtggagcgg atcgggctgt tcaattcagg cgcggttctt ttttcggtct
     2821 tgttcatgca gttccataat tttattgaga acgagaacct caagaaaaaa cttcaggaaa
     2881 tgtctgagat cgatccgctg accggggcgt ataaccggcg tttcttcaac gagtatttgg
     2941 atatcgaact caagcgtttg gaaagcccct cccgatacgc ccctgccaag aagacaaatt
     3001 ttgggatcgc gatcatagac attgatgatt tcaagatgat caatgacatg tatgggcatc
     3061 tcgttggcga tatggtgatc gcagaagtgg caggtatcat caagtccgcg atttttaagc
     3121 gcgatatttt ttgccgctat gggggcgagg agtttttggt cttgtttaca aacacgtcaa
     3181 aatcgggcgt gaagatcgcg atcgaaaaga tacgaactgc aattcagaac cacgtgttta
     3241 tgacccatgg cgagagattg aatattgaag tcacggtcag tgtagggttt gcgggtttca
     3301 gtgaagaagc caatgtctat aaactgctgg accttgcaga taagcgtttg tataaagtga
     3361 aaggggcagg gaagaatggc gcaatattgg gcgaatgact tgctctgtaa ataaaaaatg
     3421 ctccagattt tctggagcat tttttatttg aaatcgttct ttacggggtg ggtgttgcag
     3481 ccgcgctgtt ctttgcatcg agtgtggcaa cagactcagc ttgagcggtc ttttgagccg
     3541 cagcagactc ggtggcagcg gtgatgaagt tcggggtgct gggaacgcgc tgtttccaga
     3601 agtcgaaggt ttcaacggtg tagtctgtgt cgcgagcctt ggtcaaccaa tcttgcagag
     3661 cggtttcttt ggcgttctgc aattcgctgg cgctgagcgg gcgttcttgc ttcgccatca
     3721 attgaatgat gtgatagccg aactgcgaag cgacaggttc tgtggtgaaa tcgccggggt
     3781 tctgcaaagc gaaagcggcg gtttcgaatt cagccaccat ctgtcctttg ccaaaccagc
     3841 cgaggtcgcc gccttgttca gaggagctat cgctggagta ttggcgggcg aggtcggcga
     3901 agtcttcacc ggcttgcagg cgggcgatca ggtctttggc ggtttgttca tcggcaacga
     3961 gaatgtggcg cgcgcggact tgttcttcga agggcttcac atctgcggcg atgacttcca
     4021 acaatttttc gcgcaaaatt tggttgcgga agaattcgcg aatgtaggtt tcatcgaaac
     4081 catacttggt aatgttgtct ttggttttgc caagcaacac ttcataagct tcgcgggtga
     4141 agggagtagc cgtaggttct ggggtggatg tgggaccaag cgtggcggtg ggggtgacgg
     4201 tgggttcggc agtgttggtg ggcgccaagg tggcggtcgc atcgccctcc gccgtcggct
     4261 gggattcaac cgtggctgta aactcagctg tcgccgtgaa gagaggcgta gcggtcacat
     4321 cgggggtggc ggtgatgaat tgcaaaagtt ctgcggggtc ggtgggcagg tcggctggag
     4381 tgggggtgag agaaggggtg ggggtgccgt tggggtagaa gttgaagcct tcttccattt
     4441 tcttatcgat ctcttcttca ctgacggtga tgccgcgttt ttctgcttcg aggcggatga
     4501 tctcttcatc gatcatggcg tctaagacag aggtgccaac caattcgggg gtgttcaact
     4561 gggtttcaat gctgttgagc tgtgagctca tgtccatacc gaagtattgc tggtattggg
     4621 tgtattggtt gtagttgtcg atcagttgtt gtctgcggag gcgggtgcgg gcttcaaaat
     4681 ctttgagcac gatcttgttg tcgtttacgg tggcaaccgc acgattcaac tgcaggtagt
     4741 tcacatcgag ccagccgtag atgagcaaga cagcgaccac gcccaggatg ccgaagaagg
     4801 tgtaaaggat gaggcgggtt tgctgtagct cgcgttcgag gcgggcaaca tgctttttat
     4861 gcatagcggg ctttttgccc gattcgttgg tcatgggata ctcgctttgt aataaaggga
     4921 ttgcccctgt ttgtgttttg cagggatata aattcggggc atggactggt gcccatgccc
     4981 cgatgcgtta gttcgatgtt tagtcgacgc cgcgattggc gaagggcagc aacgctacgt
     5041 ggcgtgcttg cttcaccgcc gccgcaacga gacgttggtg cttggcgcag gcgccagtct
     5101 ggcggcgcgg gcgcaggctg ccttcttcgg ttacgtactt gcgaagaagg tcgacttttt
     5161 tgtagtcgat ggttagggtt ttatcggcgc agaactggca gaacttgggc ttcgcaaaga
     5221 atttgcgatc accgcgttcg ccgccgcctt cgccgcctga gcgttcaggt cgttcgggtc
     5281 gttcgtcact catgggttac tccaattgat gtcactctga gcttttgttc agaatggcaa
     5341 gaactaaaac ggaaattcct catctgctgc gccatttgac tcagcagggg aggcatctgg
     5401 ctggttgttc gcttcgcgcc ggtcgccgag catcatcatt tcattggcaa cgatctctac
     5461 gctggtatgt ttaatgcctt ctttgtcatc ccagcggcgg gtttgcaggc gtccttctac
     5521 gtagacctgc tgacctttta cgagatattg cttgcagatc tcagcaagcg ttccccacgc
     5581 cacgacattg aaccattcgg tttcgttgtg gcgctcgccg tctgcgctgt tccaggtgcg
     5641 gctgaccgcc accgtgaagg tggtgacggg tttgccactg ggggtgtagc gcatttcggg
     5701 gtctttccct aaatgtccaa tgatttgtac tttattaagt cctcggctca taagtcactc
     5761 ctaagtagaa tgccgggcaa aaacggatta tccaactaca gaaagcatgt gtcgcataat
     5821 ggactcttga taacgcaggt tgcgttcaag gtccgatgtg gcggcggggc tcatggacac
     5881 gttcaaaaga acgtattgac cttcgcggtg cttgttgatg gaatacgcca tacgacggcg
     5941 accccaaacc tcagctttgt cgatgctacc gccagcttca gtgatccaac ctttcacctt
     6001 gtcgagcacg ccattcatgg cggtctcatc caggtctggc tgaatgatgc acatcagttc
     6061 ataattacgc atgtgggaaa acctcctttc catgggtttg ttcctgttca aaccgtgggt
     6121 ttgccgggaa cggaaaggca cgtatagaaa tgtaacgtac ctaatttcgc ggcgaaagtt
     6181 taccacagga atcactttta cgggattggg ggcagaccga aaactttaag ccaacggtaa
     6241 aagtttaata aagaatttct acactaccaa gagagtggct tttttagtgc aggctttccg
     6301 ggtcttgtta ctacatcagg taattggcta actcttagat gatcttcttc tacaagtttg
     6361 tggtatttcg ctacagttat caactcttta cttacccatg cattgcgatt tttgcaagtc
     6421 aataagatgt aaactttttt attcaaatca tagcgaacaa ccctaattgg ctcaactaaa
     6481 tctgagtcgt tagtaaatac aactgcaata tcatatagat tcttaattcc atcggaaatt
     6541 aagtgagatg ctaagttaac atccgcccct ttttccttat atccacgaac atgaacaaat
     6601 tctggcttgt cttttggaga gtctcttaag ggtaatgtct ttttgaattg cgagaattta
     6661 ctgggaataa actcaaagcc cggtatagtt ttcaatgcct gtaaatatcg attttgtctt
     6721 tcaggtttcc ctaaattatc ataatattct ttagagtgag cagcaaataa cttaattttt
     6781 ccatattccc cattaggaag aaattgttta gttagtttta ataggtcaca ccaacgatat
     6841 tttgtgtttt cagtcaatcc gtgatagaaa ttaaaaccat ctacatatac tgaaattcgc
     6901 ataatattaa ttaccctcaa aacagagagc cgcataagcg gctcctgcat gcccagccgc
     6961 tgaggactgg gggacgtctt tacatattat ataaattgct caaaaaaagt caacagtaaa
     7021 tttgttctat ttacttcaca taaatcggat tgctatagat ccagccacgc cgtttgccta
     7081 ggtaattgcg gtaggcttcc acgcggtaga cgccgggttc gg
//
LOCUS       DAOQNJ010000064         7112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5657_length_7112_cov_11.329318, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000064 DAOQNJ010000000
VERSION     DAOQNJ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5657_length_7112_cov_11.329318"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLG52_03180"
     CDS             <1..189
                     /locus_tag="PLG52_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB40475.1"
                     /translation="DPYLLTIQEKTGQAKYFQLYYDQALPPALGSVVNDTVQALFAGQ
                     LTPEEATQVVEDAFVQSQ"
     gene            264..1208
                     /locus_tag="PLG52_03185"
     CDS             264..1208
                     /locus_tag="PLG52_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNB40476.1"
                     /translation="MQQLPAIPDPLKLNKKTNTPQRTQERLVIFLFLLPALTLFLVFV
                     VFPIFQSLYYSFFTWNGLGPAVDFVGLENFKKILADKVFLKALTNGVLIIIFSLAFQL
                     PFSLFLAVLVGRNLPGRVFFRTIFFMPYVLSEVITALMWLFLYNPDPERGFFNAILIT
                     LGSEPHAWLADTKLVLPAIFLALTWKYFGFHMLLFLTGLQNIPEEVEDAARIDGANSL
                     QNFFYVTLPLLGSTIRTSVYLSILGSLQQFILVWIMSKGGPVNASETLSTYMYRFGFI
                     RFQLGYGSAVAIYMFLLSLIFSLIYQRLTRQPDHLTGL"
     gene            1345..2052
                     /locus_tag="PLG52_03190"
     CDS             1345..2052
                     /locus_tag="PLG52_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNB40477.1"
                     /translation="MSNPYSVPLPPYWDNMTNILSTPVFWQMLRNSLFVMSISTIGVV
                     LLCSLAAFVFARMNFRGRGLAFNLFTLGLMFPVNIAILPVYFVLRQMNLIDTLWGVII
                     VQVAFQISGNIMILRGFFSAIPSELQDAAYIDGCTTFDFFWRILLPLARPALSAVAAL
                     TMIVSWNDFLIPLVVLNSDKLWTLPLGTMQFQGQYGSDLALVSAFIVLSAVPTIIFYI
                     FAERQIVAGLTAGAVKG"
     gene            2059..3606
                     /locus_tag="PLG52_03195"
     CDS             2059..3606
                     /locus_tag="PLG52_03195"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNB40478.1"
                     /translation="MSQTFRNPILTGCYPDPSICRVGEDYYLVTSTFEYFPGLPIFHS
                     RDLVHWRQIGYVLDRASQLDLDEIRPSAGLYAPTIRHHNGIFYVINTLVDGKTKRGNF
                     VVTATQPQGPWSEPYWLDADGIDPSLFFDEDGTCWYVGTHLHEEGHYKGHTEIFLQQL
                     DLETMRLVGETTVLWDGALKGVVWAESPHIYKVNGYYYLMIAEAGTAHHHAVTIARSK
                     NINGPYEANRSNPILTHRHLGLDYPIVGTGHADLVETQHGEWWLVLLAMRPYGGYHYN
                     LGRETFLVPARWEDDWLLASPGIGRVEFEYPAPNLPEHLWDAETPRDDFDDPTLAMQW
                     QLLRTPRDESFNLTARPGYLRLHLRPQHLAEQSNPSFIGRRQQHIHFTAEATMEFTPL
                     SANECAGMVLIQNNNFHYRFIVTQEDVPVIRLIKRSNGNEEVLASQPITAGNCVLKVT
                     AHEQNYNFYFSTDGTWHSLAENVDGRILSTSVAGGFVGAFIGLYASSNGHPSTNHADF
                     DWFEYSN"
     gene            3694..5775
                     /locus_tag="PLG52_03200"
     CDS             3694..5775
                     /locus_tag="PLG52_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HNB40479.1"
                     /translation="MSALQRFITPILLLSFILSSCGSSSTPVSATVTQTAPPPIPLPA
                     KPAFETGVYPNLFKDYLGKSDEEIQAKIDSAFHQLFYGDDATERVYYPVGSDMAYISD
                     IANEDVRSEGMSYGMMIAVQLNKKEEFDRLWKWTKTYMYQKDGGYKGYFAWHCKTDGT
                     QLAANPASDGEVWFTTALFFADGRWGSGEGIYNYRAEAQSILDTALHEDHRGDLATDL
                     FDPETRQVVFVPQLGRNSQFTDPSYHMPHFYELWARWADKDNDFWADAVTASREYLPK
                     SVHPETGLAPNYSEFDGQPVSDEYNGAFRYDAFRVGANIGMDYVWFAPSPWHVEQSNR
                     LLNFFASQGMDEYKAEYTLEGMPEVGHRSPGLMATNAVAALAADRAIGEPFVQAFWDQ
                     ALPTGQYRYYDGLLMMLGMLQVSGNFRIYEPGTAPAGQIYPTPKPEIQGKFAPLSGNA
                     LLLVGPDKKSADAYFDATVTSPGGLAFNTDLQLNNIEELDTIANTYINSTLAVHVDLT
                     GVMSDVATGKADSQINALLDALSTYKRPVFLRLAYGLDDMPNEYVSAWKKFNEQIQAQ
                     SSLSVALIWQADSCGEASMADFYPGDELVDWVSTNYECADATLQFAREHYKPVMLEAT
                     SLGGWNEWYAPFFKFVMDNNDVVRAVIYINADKSQLTDTDIIKSWKTETKQPFWLHAK
                     PNLFGNLGFDN"
     gene            5822..>7112
                     /locus_tag="PLG52_03205"
     CDS             5822..>7112
                     /locus_tag="PLG52_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNB40480.1"
                     /translation="MSVDNINVKVPYLDTTLPLKERVKDLVGRLTLEEKVGLMSHPAH
                     GIPRLNIPAYNYWSEALHGVARNGRATVFPQAIAMAATWDKDLILKVATAISDEGRAK
                     YHEALRRNGYTNQYQGLTFWSPNINIFRDPRWGRGQETWGEDPFFTGEMAAMFVRGLQ
                     GDDPKYLKAAACAKHFAVHSGPEKDRHTFNAIVTKRELYDTYLPAFKKLVTEAKVESV
                     MGAYNRTLDEVCCASQLLIEDILRGEWKFDGHYVSDCFALSDFHLNHKVTEDAAESAA
                     LALKHGCDLGCDHVFNEIPTAIERGDTTEALVDRALIRTFTTRFKLGMFDPAEEVPYA
                     SIPTDVVACDEHRQLAYRTATEAVVLLKNKNNVLPIQSTTKKIFVTGPTAASLEVLLG
                     NYYGFNNKMITLLEGLVGRIPEGMGLEYHSGTPLKHTR"
BASE COUNT         1817 a         1817 c         1594 g         1884 t
ORIGIN      
        1 gatccatatc tgctaaccat tcaggaaaag acgggtcaag caaagtactt ccagctttat
       61 tatgaccagg cattacctcc tgcacttggt tcggttgtga atgacactgt tcaagcattg
      121 tttgctggac aactcacacc tgaagaggcg acacaggtcg ttgaggatgc ttttgtccaa
      181 tcgcagtaag aatgcataac taagaagaag tgcagggagg agaaaacctc tctgcacttt
      241 ctttaccgcc aaggagtttt tgtatgcagc agcttccagc cataccagac ccgctgaaat
      301 taaacaagaa aacaaataca cctcaaagaa cccaggaaag attggttatc ttcctattcc
      361 ttttacccgc cctaaccctc tttcttgttt ttgtagtatt tcccatcttc caatccttat
      421 attacagttt ctttacttgg aatggtctgg ggcctgccgt cgattttgtc gggttggaga
      481 atttcaagaa aatcttagct gataaagtgt ttttgaaggc actcacaaat ggagtgctga
      541 tcattatttt ctctttagca tttcaactcc ccttttcatt atttctggca gtcttggttg
      601 ggcgtaattt accgggtcgc gttttttttc gcactatctt ttttatgccc tatgtgcttt
      661 cagaggtaat cacggctcta atgtggctgt ttctttacaa cccagacccg gaacggggtt
      721 tctttaatgc aattttgata actctgggaa gtgaacctca tgcctggctt gcagatacca
      781 aacttgtctt acccgcaatc tttttagcac taacgtggaa gtactttggc tttcacatgc
      841 ttttgtttct aacagggcta caaaatattc cggaagaagt tgaagatgcc gcacgcatag
      901 acggagccaa ttcgcttcag aatttcttct atgtcacttt gcctttgctg ggcagcacaa
      961 ttcgcacttc ggtctacctc tcgatattgg gttcattgca acaatttatt ttggtatgga
     1021 tcatgtccaa aggcggacct gtaaatgcca gcgaaaccct ctcaacctac atgtatcggt
     1081 ttgggttcat acggtttcaa ttgggatacg gctccgcagt tgccatttac atgttcctgc
     1141 tttccctcat cttttcattg atttatcaac gccttacccg tcaacctgat catttgacgg
     1201 gtctataaaa gggatacttt catgactcga gcccgaaaca ttacccgttt ttttcaatac
     1261 acaattcttt cgataattgt tgtgctcgtt ctgttacctg tgataatgtt ggtttttggg
     1321 gcgttgaaaa cgcgcggcga attaatgtca aacccctata gtgttcccct tcctccctat
     1381 tgggataaca tgaccaatat tttatccaca cccgtgttct ggcaaatgct ccgcaacagt
     1441 ctatttgtca tgtccatttc aaccatcggt gtggtgttgt tatgcagttt agccgcattt
     1501 gtctttgcac gtatgaattt tcgaggtcga gggctggcgt tcaacctatt cacactcggg
     1561 ctcatgtttc ccgtcaatat tgccatattg ccagtgtact ttgtgctccg ccaaatgaat
     1621 ctgatcgaca ctttatgggg tgtgattatc gtacaagttg cttttcaaat atccggcaat
     1681 atcatgatct tgcgtggttt cttcagcgcg atccctagtg aactacaaga tgctgcctat
     1741 atagacggct gtactacctt tgactttttc tggcggatcc tgttgccctt ggcgcgcccg
     1801 gcattatccg cagtagccgc attgaccatg atcgtaagct ggaatgattt cttaatcccc
     1861 ctggttgttt tgaacagtga caaattatgg acactgccgc ttggaaccat gcaatttcaa
     1921 gggcaatatg gctcagatct tgctctagta tctgccttca tcgttttgtc ggctgttcca
     1981 accatcatct tctatatctt cgctgaacgt cagatcgttg ccgggctgac agctggcgcc
     2041 gtaaaaggat aactcataat gtcgcaaacg tttcgcaacc ccattctcac aggttgttat
     2101 cccgaccctt ccatctgccg ggtgggtgaa gactattacc ttgtaacttc caccttcgaa
     2161 tattttccag gactcccgat ctttcatagc cgagacctgg ttcactggcg gcaaattgga
     2221 tatgtcctcg accgcgcatc tcaactcgac ctcgatgaga ttcgcccatc tgcgggttta
     2281 tacgccccga caattcgcca tcataatggt atcttctacg tgatcaacac actggttgac
     2341 ggaaaaacaa agcggggaaa cttcgtcgta acagcaaccc aaccacaagg tccatggagc
     2401 gaaccgtatt ggctcgacgc agatggcatc gatccatcgc tcttctttga tgaggatggt
     2461 acatgttggt acgtcggcac acacctccat gaagaagggc attacaaagg tcacaccgaa
     2521 atttttctac aacaacttga ccttgaaaca atgcgtttgg tcggtgagac aacagtgttg
     2581 tgggatggcg ccctcaaagg tgttgtatgg gcagagtcgc cacatattta caaggtcaac
     2641 ggctattatt atttgatgat cgccgaagcc ggaacagccc accaccatgc ggtcacgatt
     2701 gctcgcagca aaaacattaa tggaccctac gaagcaaacc gcagtaaccc cattctgaca
     2761 catcgccatc tcggtttgga ttatccgatc gttggcactg ggcatgccga tctggttgaa
     2821 acccaacatg gggaatggtg gctggtgctg cttgccatgc gaccttatgg cggttatcac
     2881 tataacctgg gacgcgaaac ctttcttgta cctgcacgtt gggaagatga ctggttactg
     2941 gcaagccctg gcattggacg tgtagagttt gaataccctg cacctaattt acctgagcat
     3001 ttatgggatg cagagacacc gcgtgatgac tttgatgatc caacgcttgc catgcaatgg
     3061 cagcttttac gcaccccgcg tgatgaatca ttcaacctaa cagcccgacc gggttatttg
     3121 cgattgcact tgcgcccaca acatctggca gaacagagca accccagttt tatagggcgc
     3181 agacagcagc acattcattt cactgcagaa gccaccatgg aattcacgcc gctttcggca
     3241 aatgaatgtg caggtatggt tctcattcaa aacaataatt tccattatcg ctttattgtc
     3301 acgcaagaag atgtgcccgt gattcgcctg atcaaacgtt ccaatgggaa tgaggaagtg
     3361 ctggcatcac aaccgatcac agccggaaat tgtgtgctca aagttacagc acacgaacaa
     3421 aattacaatt tttatttttc aacggatggc acctggcatt ccttggcaga aaatgtagat
     3481 gggcgcattc tgagcacatc tgttgcgggt ggattcgtcg gcgcgttcat tgggttgtac
     3541 gccagcagca acgggcatcc cagcaccaac catgctgact ttgattggtt tgaatactcg
     3601 aattaaaacc ttacttgtat tcatactgaa tacaagtaac cttggttaat tgtcgggttt
     3661 atgatgaaaa aaaatctatt taggattgag accttgtctg cgcttcaacg tttcatcact
     3721 cccatacttt tactttcctt catcctctcc tcctgcggat catcctccac gccggtatct
     3781 gccactgtga cccaaaccgc cccacctccc atacctctcc ctgccaaacc tgccttcgag
     3841 accggtgtct accccaactt gttcaaagac tacctcggca aaagtgacga agagatccaa
     3901 gccaagatcg actccgcctt ccatcaactc ttctacggtg atgatgcgac cgaacgcgta
     3961 tattatcctg tcggttctga tatggcatac atctccgata ttgccaatga agacgtacgt
     4021 agtgaaggga tgtcctacgg tatgatgatc gccgtacagt taaacaaaaa agaagaattc
     4081 gaccgccttt ggaaatggac aaaaacatac atgtatcaaa aggatggcgg atacaaaggt
     4141 tattttgcct ggcactgcaa aacggacggt acccaactcg ccgccaaccc tgcttctgat
     4201 ggtgaagtct ggttcaccac ggccctcttc ttcgcagatg gacgttgggg cagcggcgaa
     4261 ggtatctata attatcgcgc tgaagcacaa tccattttgg atacagcatt gcatgaagat
     4321 catcgcggcg accttgccac agatctcttc gaccctgaaa ccaggcaagt tgtctttgtt
     4381 ccacaattag ggcgcaactc gcaattcaca gacccttcgt accacatgcc gcacttctat
     4441 gagttgtggg ctcgctgggc agacaaagat aacgacttct gggcagatgc tgtcacagcc
     4501 agccgtgaat atcttcctaa atccgtccac cctgaaacgg gactcgcccc aaattattca
     4561 gagtttgatg gtcaacctgt cagtgatgaa tacaatggcg ccttccgtta cgatgccttt
     4621 cgagttggtg caaatatcgg catggattat gtctggtttg cgccaagtcc atggcatgtg
     4681 gagcaatcga atcgtctatt gaatttcttt gcatcgcaag gcatggatga atacaaagct
     4741 gaatacacgc tggaaggcat gcccgaagta ggtcatcgct ctccaggctt gatggcgacc
     4801 aatgccgttg ctgctcttgc cgcagatcga gccattggcg agccatttgt ccaagcattt
     4861 tgggatcaag cactgcccac aggtcaatat cgttattatg atggcttgct gatgatgttg
     4921 ggtatgttac aggtcagcgg caacttccgc atttacgaac ccggcaccgc acctgccggg
     4981 cagatctacc cgacccccaa gcctgaaatc cagggcaaat ttgctccatt atcagggaat
     5041 gccttattgt tggttggacc cgataaaaaa agtgcagatg catacttcga cgcaacggtc
     5101 acttcacctg gaggcttggc gttcaacacc gatttgcaat taaacaacat tgaagaattg
     5161 gatacgattg caaatacata catcaatagc actttagctg ttcatgtgga tttgacaggg
     5221 gttatgagcg atgtcgccac aggcaaagcg gattcacaga tcaatgccct gcttgatgca
     5281 ttaagcacct ataaacgccc tgtatttctg cgccttgctt atgggcttga tgatatgcct
     5341 aatgaatatg tatcagcctg gaaaaaattc aatgaacaga ttcaggctca atcgagtctg
     5401 agcgtggctc tcatctggca ggcggattcg tgcggtgaag catccatggc agatttctac
     5461 cccggtgatg agctggtcga ttgggtcagc acaaattacg aatgcgccga tgccaccctc
     5521 caatttgcgc gcgagcatta caaacccgtg atgcttgaag caacatcgct gggcgggtgg
     5581 aatgaatggt acgcgccatt cttcaagttt gtgatggata ataatgatgt tgtgcgcgca
     5641 gtgatctata tcaatgcaga caaaagtcaa ttgacagata ccgatattat caagagttgg
     5701 aaaacagaaa ctaagcagcc cttttggcta catgccaaac caaatttatt tggcaacttg
     5761 ggttttgata attgaccgta gaacactgtt gcggtcatta aagatttcaa aggagcactc
     5821 catgagtgtg gacaatataa acgtaaaagt accctattta gatacaaccc ttcctcttaa
     5881 agaacgggta aaagatttag tgggacgcct gacccttgaa gaaaaagtgg ggctcatgag
     5941 tcacccggcg catggcattc cgcgcctgaa cattcccgcc tataactatt ggagcgaagc
     6001 cttgcacggc gtcgcccgca atggacgagc caccgtcttc ccgcaggcaa ttgctatggc
     6061 tgccacctgg gacaaagacc tgatcctcaa agtcgcaact gccatcagcg acgaaggacg
     6121 cgcaaaatat cacgaagccc tgcgacgcaa tggctacacc aaccaatatc aaggattaac
     6181 cttctggtca ccgaacatca acattttccg tgacccgcgt tgggggcgtg gacaggaaac
     6241 ctggggcgaa gatcccttct ttactggtga aatggcggca atgttcgtac gcggcttgca
     6301 aggcgatgac ccaaaatatc ttaaagccgc agcatgcgcc aaacattttg ctgtccattc
     6361 gggtcccgaa aaagaccggc acaccttcaa cgccatcgta acaaaacgcg aactgtacga
     6421 tacctacctg ccagctttta agaagctggt caccgaagcc aaagttgaat ccgttatggg
     6481 cgcatacaat cgcacactcg atgaagtctg ttgcgccagc cagttgctta ttgaagatat
     6541 cttgcgcggc gagtggaaat ttgatgggca ctatgtctca gactgcttcg cactgtcaga
     6601 ttttcatctc aaccacaaag tcacagaaga tgcggcggaa tcagccgcgc ttgcattgaa
     6661 gcatggctgc gacctcggct gcgaccacgt cttcaatgag attcccaccg ccatcgaacg
     6721 tggcgataca accgaagcgc tcgtagaccg cgccctgatc cgcactttta ccacccgctt
     6781 caaacttggc atgttcgacc cagccgaaga agtaccctac gcctccatcc ccaccgacgt
     6841 ggttgcatgc gacgaacatc gtcagctcgc ataccgcact gccaccgaag cggtagtgct
     6901 gctaaaaaat aaaaacaacg tcctgccgat tcaatcaacc accaaaaaga tattcgtcac
     6961 aggtcccaca gccgcaagcc tcgaagtact cctcggcaac tattacggct tcaacaacaa
     7021 aatgattacc ttgcttgaag gtctggttgg gcgcatcccc gaaggcatgg gtttggaata
     7081 tcactccggc acgccgttaa aacacacgcg tg
//
LOCUS       DAOQNJ010000065         7005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5812_length_7005_cov_12.504604, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000065 DAOQNJ010000000
VERSION     DAOQNJ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_7005_cov_12.504604"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..>1788)
                     /locus_tag="PLG52_03210"
     CDS             complement(4..>1788)
                     /locus_tag="PLG52_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNB40481.1"
                     /translation="FWSPNVNIFRDPRWGRGQETWGEDPFFTGEMAAEFVKGLQGNDP
                     TYLKTAACAKHFAVHSGPEKDRHSFNAIVTKRELYDTYLPAFKKLVTEAKVEAVMGAY
                     NRTLDEVCCASKLLIEDILRGEWGFDGHFVSDCMALSDFHLNHKVTKDAAESAALALK
                     YGCDLGCDHVFNELPAAIERGDTTEALVDRALERTLRTRFKLGMFDPAEDVPFASIST
                     DVVASDAHRQLAYKAATEAVVLLKNQNNVLPIKPSTKKVFVTGPTATSVEVLLGNYYG
                     FNNKMVTLLEGIVGRIPEGMGLEYTAGTPLKHAREIKESWAPQMAQSADFAIVCAGFN
                     SFLEGEEGESLLSAQNGDRESISLPQSQIDYIKELAIHGARIILVLTGGSPIALGEVE
                     DLVEAILFVWYPGMEGGRAVADVLFGDVSPAGKLPITFPKSLDQLPAFDDYGMTGRTY
                     RYMTAEPLYPFGFGLSYTCFEYSNLKLDKTNLALGDSLTLNLTVRNSGGSDSAEVVQY
                     YLSDLHASTIVPLHHLIGFERVNLKAGESKTLTFTITPDMMSFYNDDGKLTLEPGEFR
                     VEVGSCSPGKRGQELGAPAPVTALFEVK"
     assembly_gap    1789..1888
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1942..3108)
                     /gene="xylA"
                     /locus_tag="PLG52_03215"
     CDS             complement(1942..3108)
                     /gene="xylA"
                     /locus_tag="PLG52_03215"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNB40482.1"
                     /translation="MSKYSPSPENKFTFGLWTVGNVGRDPFGSPVRDGKSPADLVRLL
                     GEVGAYGVNFHDNDLIPIDATPSERDAILKEFKKALADTGIIVPMATTNLFSEPIFKD
                     GAFTSNDSKVRAYALQKTMNAIDLGVELGAKTYVFWGGREGTESDATKDPITSIKRSR
                     EAMNFLCEYVLDKKYDLKFALEAKPNEPRGDIYNSTTGHMLAFIETLDHPDMVGVNPE
                     VAHEHMAGLNFVHGVAQALEAGKLFHIDLNDQAFGRYDQDFRFGAVNIKSAFFLVKLL
                     EDNKYNNPRHFDAHAYRTEDYEGVKDFARGCMHTYLVLKEKAAQWNADKEIQSLVAEI
                     NADDGSMAQYFGKYSSAKASALKAQAFDRNAIASRGLKYERLDQLTIDVLLGVR"
     gene            complement(3275..4150)
                     /locus_tag="PLG52_03220"
     CDS             complement(3275..4150)
                     /locus_tag="PLG52_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNB40483.1"
                     /translation="MENVRESDKAMLNNYKFTTTVLRILVYFMLFALLVVTIVPIWLL
                     IVNATRSTTEIQQGLSIMPSTHLIENYNILLGKGLNLPRGFANSTFLAVSSTVVSVYF
                     SMLTAYGIVVYDFKGKHAFSNFIVVLVMIPMQLSIIGFYQYMSKLGLTDNYASLILPL
                     IANAGGVFFGKQYLESMIIQDLIDAARIDGASEMAIFHQVMMPLAIPGAVTMGIFAFI
                     TSWNNFFNAFILISSIEKYTLPMLVQTLRGDVYRTEFGAIYLGLAATIVPIILLYIFF
                     SRYVVSGIAIGAVKE"
     gene            complement(4134..5072)
                     /locus_tag="PLG52_03225"
     CDS             complement(4134..5072)
                     /locus_tag="PLG52_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNB40484.1"
                     /translation="MGLIKLKKHQYGYIFIAPFIIGFLAFGLYPVYNTLALSFTDATL
                     MTKSAHFVGFKNFERLFADDVFLGAVKNTWLLWALNFIPQVGIAMLLSVWFTDARLKI
                     KGVGLWRAVFFLPNLLVPASVAALFFAFFSFYGPVNQFMVRFGFLPEAMHYLQSVPVT
                     RGLVIFIQWWMWFGQTVIIVMAGMTSIPVSLYEAAMVDGATTSQMFRRITLPLLKPIL
                     IYIFVTSLVGGMQMFDIPFLLTDGRGSPNSSIQTNNILMYMKFASSKGHIGAASSVGV
                     LVFLMTSICALGIFYFLRERDTVSEKPKKSLFGKRS"
     gene            complement(5164..6627)
                     /locus_tag="PLG52_03230"
     CDS             complement(5164..6627)
                     /locus_tag="PLG52_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB40485.1"
                     /translation="MKRLSYILSLLVVVSMFLAACAPAATEEAPAATEAAAEATDAPA
                     ATEAAPAELSGTLNVWSFTNEIRTMAIAFEGVHPNVDVVYTMIPMTNGEYQTKVKAAA
                     GTADSPDVVALEAAFVKEWVESDLLADQSDLLPLAEELKTFPSVVEVGTYDGITKAYS
                     YQATPGAFFYRRSIAKECLGTDDPDAVQAMVADLDKFVETAAKIKECGTGDYFTVGTS
                     GEMFNPFLANREQPWVVDGELVIDPMVLEYVKFAKLMRENGYESQATQWSEGWFAGMN
                     DTLKDANGTPKKVFSYFLPTWGLPYVLIPNSKSDTTDTGGDWAMINGPMAYQWGGTWV
                     GALEGSPNAELAKEFIKFVALNEDNLTNWATGVYTNEYLKAIDPETPDDQAQAPGDFV
                     SSQVVVEKITSTFDGSDLAKWLGGQNSYAAFAKAAPDVNGALLTGSDDAIQRALNDPL
                     ASYLNGDITEAEMYTQWLDAVRNEFPDLVIPEPPVTE"
BASE COUNT         1809 a         1614 c         1777 g         1705 t
ORIGIN      
        1 tttttatttg acctcgaaca acgccgtgac cggtgctgga gcgcctagtt cttgtccgcg
       61 tttgccgggg gagcagcttc ccacttccac gcggaactct ccaggttcga gagtgagttt
      121 gccatcatca ttatagaagg acatcatgtc tggtgtgatg gtgaaggtca acgttttgct
      181 ttcgcctgct ttgagattca cacgctcaaa accaatgaga tgatggaggg gaacaatggt
      241 cgaggcgtgg aggtcgctca ggtagtattg aaccacttcg gctgagtctg agccgccgct
      301 gttgcggacg gtcaggttga gggtgagaga gtcgccaagg gctagattcg ttttatcgag
      361 ttttagattc gaatattcaa aacaagtgta gctcaagcca aacccaaatg gataaagcgg
      421 ctcggcggtc atgtaccgat aagtgcgccc agtcatgccg taatcgtcga aggcgggaag
      481 ttgatcgagc gacttgggga aggtgatggg taatttgcca gcgggagaaa catcgccgaa
      541 gagcacatcg gcaacagcgc gaccgccttc catgccggga taccaaacga agagaatggc
      601 ttccaccagg tcttctactt cgcccaaggc gattgggctg ccacctgtga gcacaaggat
      661 gatgcgcgcg ccatgaatag cgagttcttt gatgtaatca atttgacttt gcgggagcga
      721 gatactttcg cggtcgccgt tctgtgcaga gagtaacgac tcgccttctt caccttcgag
      781 aaatgagttg aagcctgcgc acacaatcgc aaaatctgcc gattgtgcca tttgcggcgc
      841 ccaagactct ttgatctcac gtgcatgttt caagggcgtg ccggctgtat attccaaccc
      901 catgccttca ggaatgcgcc ctacaatgcc ttccaacaga gtgaccatct tgttgttgaa
      961 gccgtaataa tttccaagca acacttcaac gcttgtggct gtgggacccg tgacaaaaac
     1021 ttttttcgta gaaggtttga taggcagaac gttattctga ttcttgagca acaccaccgc
     1081 ttcggttgcg gctttgtatg ccaattggcg atgagcatca cttgcaacca catccgtaga
     1141 gatggatgcg aaaggcacat cttcggcggg gtcaaacatg ccgagtttga agcgcgttcg
     1201 caatgtgcgt tcgagggcgc ggtctaccaa ggcttcggtc gtatctccgc gttcgatggc
     1261 ggcgggaagt tcattgaaga cgtggtcaca gccaaggtcg cagccatatt tcaacgccag
     1321 tgcagcggat tccgccgcat ctttcgtgac cttgtggttg agatgaaaat ctgacagcgc
     1381 catgcagtct gaaacaaagt gcccgtcaaa gccccattct ccgcgcaaga tatcttcgat
     1441 cagaagtttg ctggcacaac acacttcgtc caaggtgcgg ttataggcac ccatcacagc
     1501 ttccactttg gcttcggtga caagtttttt gaaagcaggc aggtaggtgt cgtataactc
     1561 acgtttggtt acaatggcat taaaggagtg gcggtctttt tctggaccag aatgcacagc
     1621 aaagtgtttt gcacaggcgg cggttttgag ataggtaggg tcattgcctt gcaagccttt
     1681 gacgaattca gccgccattt caccggtaaa gaagggatct tcgccccagg tttcttgtcc
     1741 gcgcccccaa cgtgggtcac gaaatatatt cacattcggt gaccagaann nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnag atagatgggc ttttccgcga tattcgagtt
     1921 actcattcaa gggctcctac gttatcgcac gccaagtagt acgtcgatgg tcaactggtc
     1981 gaggcgttcg tatttcaatc cgcgagaggc gatggcattg cggtcgaagg cttgagcttt
     2041 gagagcgctg gctttggctg aggagtactt gccaaagtat tgcgccatgg aaccgtcatc
     2101 tgcattgatc tctgcaacga gagattggat ctctttatcc gcattccatt gtgcggcttt
     2161 ttctttaaga acgagatagg tgtgcataca gccacgcgcg aaatctttaa ccccttcgta
     2221 gtcttcggtg cgatatgcat gagcatcaaa gtgacgtgga ttgttgtatt tattgtcttc
     2281 gagtaatttg acgaggaaga aagcgctctt aatgttgact gcaccaaagc ggaagtcttg
     2341 atcatagcga ccgaaggctt ggtcgttcaa gtcaatgtgg aagagtttgc ccgcttccaa
     2401 cgcctgagct acgccgtgta cgaagttgag ccctgccatg tgttcatgtg ccacttcagg
     2461 gttgacgccg accatatcgg gatgatcgag ggtttcaatg aatgccaaca tatgcccagt
     2521 ggtggagttg taaatgtcgc cgcgcggttc attgggtttg gcttcgagcg caaacttgag
     2581 gtcatatttt ttgtctaata catattcgca gaggaagttc atcgcttcgc gcgagcgttt
     2641 gatggaggtg atggggtctt tggtggcgtc actctctgtc ccttcgcgac cgccccagaa
     2701 tacataggtt ttggcaccga gttcaacgcc gagatcgatg gcgttcatcg tcttctgcaa
     2761 cgcatatgcg cggacttttg agtcgtttga tgtaaatgca ccatctttga aaatgggttc
     2821 agagaacaaa ttggtggttg ccatcggcac gatgatgcct gtatcggcaa gcgccttctt
     2881 gaattccttc agaatggcat ctcgttcaga gggcgtagca tcaatgggaa tcaggtcgtt
     2941 atcatgaaag ttgacaccgt atgcgccaac ttctccgagc aggcgaacca agtctgcggg
     3001 agatttgcca tcgcgaaccg ggctaccgaa tgggtcgcgt ccaacattgc caacggtcca
     3061 cagaccgaag gtgaacttgt tttcagggga tggggaatac ttggacatta gattactcct
     3121 tggtttttac tgatttttag caatactctc ccagccccgc ctccacatgg aggcggggaa
     3181 cgcataagga gatagaacaa atgaatgaag caaaactaag atggagggta aagtgtaaga
     3241 gggtagaaca agaaaagtta aagtatgagg cgctttattc ctttacagct ccaatggcaa
     3301 tgccgctaac cacgtatcgt gagaagaaaa tataaagaag aatgatcgga acaatggtgg
     3361 cagccaagcc aaggtaaatg gcgccaaatt cggttcgata cacatctccg cgcagggttt
     3421 gcaccaacat gggaagtgtg tatttttcga tcgaagagat caggatgaag gcgttgaaga
     3481 agttattcca agatgtaatg aaggcaaaga tacccatggt caccgcaccg ggaatggcaa
     3541 gcggcatcat cacttgatgg aatattgcca tttcactggc gccgtcgatt cgagcagcat
     3601 cgatcaagtc ttgaatgatc atggattcta aatattgttt tccaaagaac accccgcctg
     3661 cattggctat cagcggcagg atcagagatg cgtagttatc tgtcagcccc agttttgaca
     3721 tgtactgata aaagccgatg atcgagagct gcatgggaat catgaccaac acgacaatga
     3781 agtttgagaa ggcatgtttg cctttgaaat catagaccac aatgccataa gcagtcaaca
     3841 tggaaaaata gacagacacc actgtggacg ataccgctaa aaaggtgcta tttgcaaacc
     3901 ctctgggaag gttcaaccct tttccaagga ggatgttgta attttcaata agatgcgtac
     3961 tgggcataat gctgagaccc tgttggatct cggtggttga gcgggttgca ttgacgatca
     4021 atagccagat cggcacgatc gtaacgacca gtaacgcgaa cagcataaaa taaacaagaa
     4081 ttcgaaggac ggtggttgtg aatttgtaat tgttcaacat ggctttatca ctttcacgaa
     4141 cgttttccaa agagagactt cttgggtttt tctgaaactg tatctctttc gcgcaagaag
     4201 tagaaaattc ccagtgcaca aatgctggtc ataagaaata ccagcacacc aaccgatgaa
     4261 gctgcaccaa tatgtccctt actactggcg aacttcatgt acatcaagat gttgttggtt
     4321 tgaatggagc tattgggtga gcctcttcca tccgtgagaa ggaaaggaat atcgaacatt
     4381 tgcattccgc ccacaagaga tgtgacaaag atatagatca aaatgggctt gagcaaaggc
     4441 agagtgatac gacggaacat ttggctggtg gttgcgccat ctaccatggc agcttcatat
     4501 aatgaaacgg ggatggacgt catccctgcc atgacgatga tgacagtttg cccgaaccac
     4561 atccaccatt gaatgaagat aaccaatccg cgcgtaaccg gtacgctttg gaggtaatgc
     4621 atggcttcgg gtaaaaaacc aaaacgcacc ataaattgat tgacaggtcc gtaaaaagaa
     4681 aagaaggcga aaaataaagc cgctacagag gcaggcacta gcaggttggg caaaaagaaa
     4741 accgctcgcc ataaccctac ccctttgatc ttaagtcttg catcggtgaa ccatacggat
     4801 aacaacatgg cgatcccgac ctgtggaata aagttcagcg cccaaagtaa ccaggtattc
     4861 tttacagcac caaggaagac gtcatcagca aacaaccgct caaaattttt gaaacctaca
     4921 aaatgagcac ttttggtcat tagagttgca tcagtaaagc tcaatgccaa agtgttatag
     4981 accgggtata agccgaaggc aagaaaacca ataatgaacg gtgcaataaa aatatacccg
     5041 tattgatgtt tcttaagttt gataagtccc atgacgtctc ctcaacaaaa taaaaattgt
     5101 cagtggtgtt ctgacgggtt gggtcagtgt tttgcactga cccaaccact acttcacatt
     5161 ctcttattca gtgacaggag gttcagggat aacgagatcc gggaactcat tgcgaactgc
     5221 atcaagccac tgggtgtaca tttcagcttc agtgatgtcg ccattgaggt agctggcgag
     5281 cggatcattg agggcacgct gaatggcatc gtcagaaccg gtcaacaaag caccgttgac
     5341 atctggagca gccttggcga aagcggcata ggagttctgt ccgccgagcc atttggcaag
     5401 atctgaacca tcgaaggtgc tggtgatctt ctcaaccaca acctggctgg aaacaaagtc
     5461 gccaggagcc tgagcttgat catctggggt ttcaggatcg atcgccttga ggtattcgtt
     5521 ggtgtaaacg ccggttgccc agttggtcag gttatcttcg ttaagagcta cgaacttgat
     5581 gaattcctta gccaattcag cattggggct gccttccaaa gcgccaaccc aagtaccacc
     5641 ccattgatac gccataggac cattgatcat cgcccagtcg ccgccggtgt cggtggtgtc
     5701 agacttggag ttggggatca acacgtaagg aagaccccaa gtggggagga agtagctgaa
     5761 gaccttcttc ggggtgccgt tggcatcctt caaagtatcg ttcatgccag cgaaccaacc
     5821 ttcactccac tgagtagcct gagattcgta gccgttctcg cgcatgagct tggcaaactt
     5881 gacatattcc aagaccatcg ggtcgatcac gagttcacca tccacaaccc agggctgttc
     5941 gcgattggcg aggaaggggt tgaacatttc accagaggta cccacggtga agtaatcgcc
     6001 ggtgccgcat tctttgatct tggcagcggt ttcaacgaac ttatcgaggt cagcaaccat
     6061 ggcttgcaca gcatcaggat cgtcggtgcc gaggcattcc ttggcaatgc tgcggcgata
     6121 gaagaacgcg ccgggggtcg cctgataaga atacgccttg gtgatgccat catacgtacc
     6181 aacttctaca acagagggga aggtcttgag ttcttcagcc aaaggcagca agtcgctctg
     6241 gtcagccaaa agatcagatt caacccattc cttgacgaaa gccgcttcaa gcgcgaccac
     6301 atcgggggaa tccgcggtac cggcagcagc cttaaccttg gtctggtatt caccattggt
     6361 catcgggatc atggtgtaaa caacatcaac attcgggtga acaccttcga atgcgatcgc
     6421 catggtgcga atttcattgg tgaacgacca aacattcaaa gtaccagata attcagcagg
     6481 ggcagcttca gtggctgcag gggcatcagt agcttcagcg gctgcttcag tggcagcggg
     6541 ggcttcttca gtcgctgctg gtgcgcatgc tgctaagaac atgctaacga caaccagcag
     6601 agataagatg taagataatc gtttcatctt cgttctcctt gttaataatt atggtggtat
     6661 atttccaagt tgaagtccgc atccttccat agagtcgggc ttcgacttct tggcgtccga
     6721 tgtcattcag atatcggacg ctcgcatttc aagtacatga aatttgcgag taacaatatg
     6781 tgatgccagc cacctccttt ttcatttgac ggaataaatt ccaaactgca ctactgtacg
     6841 gatcctacag accggctcta aatcggacct gtaagtgggt accaaaacct gggctttcac
     6901 ttactcttag ggagtttccg gtaacgttac cggaaactcg tataaaaaaa tccatttccg
     6961 tccaccacta tcttacgatt ggacggtttg gattaatttt tgaca
//
LOCUS       DAOQNJ010000066         6971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5858_length_6971_cov_7.025593, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000066 DAOQNJ010000000
VERSION     DAOQNJ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5858_length_6971_cov_7.025593"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLG52_03235"
     CDS             <1..701
                     /locus_tag="PLG52_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB40486.1"
                     /translation="GEPSYVWRDGQKRRLNMIAKFAAERMQGKVLENGCGVGMYVEKM
                     TELGATVTGLEYDFERACDAHVNSPRIVNAAGEFLPLPSSTFDLILSHEVIEHVQDDR
                     SAIAEMVRVMKVGGRAVIFCPNRGYPVETHGIFWNGKYYFGNKLFVNYLPRVWRDKLA
                     PHVRVYSRRDMQKLFEGLPVKFIERTIIFGAYDNIIARLGVFGKFIRAVLQFMEATPL
                     KILGLSHLWVVEKI"
     gene            complement(736..1161)
                     /locus_tag="PLG52_03240"
     CDS             complement(736..1161)
                     /locus_tag="PLG52_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40487.1"
                     /translation="MPQPLFAGLIVDEDGNPVQAASIGSEPAYVIDDAGFKRHIPAEQ
                     VDRAVLNQMADMMKGSEDVLSEQTAKMLGQEDPFSKAMIANQLKNIDKQFDALMKTGF
                     PEELRAYLGMMGFKIVINYHGEVVRVEQPGTSGGDEGDE"
     gene            complement(1231..1806)
                     /locus_tag="PLG52_03245"
     CDS             complement(1231..1806)
                     /locus_tag="PLG52_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40488.1"
                     /translation="MNQIFQGNQYLLKRQVFALTGKFRVFDTAGRLLLFSEQKMFKLR
                     EDIRVYADEAKTQEVLAIKARQIVDFSAAYDVIDSATGQKVGAMRRKGLKSILRDEWE
                     VLDVNDNVRGVLVEDSMGLALLRRFLTNLVPQNYDMLFGETRVGDFKQNFNPFTYELN
                     IDFSMDTAGQVDRRLGIAAGILLAAVEGRQQ"
     gene            complement(1867..2889)
                     /locus_tag="PLG52_03250"
     CDS             complement(1867..2889)
                     /locus_tag="PLG52_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNB40489.1"
                     /translation="MTIHQLSRRDFLKLSASGALGAVLSELGVDRALAAPPTSKGLAI
                     FSGVPIYDAPVANANKVELIGKDQVIELKAEVQGDYFGNQFNTTWYEVDGGFVYSAPI
                     LPVEEHYQKPVYSVPTDGQLAEVSVPYTLTYRSPFFYAKNSHRLYYESTHWVNKVIVN
                     REEKGVWYEVYNKILKETVYIPSYNMRLIPNEELTQISPEVPPEEKLLHVDIATQMVT
                     AFEGEKVVLSVRCASGQHGTDTPKGEWSTYHKGPSIHMTNQGDAVANIYNLPGVPWCS
                     FFTGSGHAFHGTYWHNDYGRLRSNGCVNLPSSVAKWVYRWTNPVVPYNVDYVNLPGQG
                     TKVIIE"
     gene            complement(2945..3967)
                     /locus_tag="PLG52_03255"
     CDS             complement(2945..3967)
                     /locus_tag="PLG52_03255"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNB40490.1"
                     /translation="MKTLSRRDFLKLSGLGLASFMVPPLNFDSDDPFDLQQGRVTVRT
                     VWVYDKPTLEATKVKLLQRDAIFNITNTAVTEDIDSHNRIWYQVGTDGYVPSGNIQPV
                     RTMLSQPYTANIPIEGLLAEVCVPYTDAFVEPSTESKVGYRIYYETTHWVKALVAGKT
                     DGQPWYQIRDDKWDELYYVRADHIRIMTPEELAPITPEVPLKEKKIVVQLEQQLVTAY
                     EMGVPVFSVPVSTGARLRSGTYTTPSGNFITYYKRPSRHMAAGDIAASGFDLPGVPWV
                     QYLTKSGISFHGTFWHNDYGRPRSHGCINMSCSAAKWLFLWSSPSVPVNKEFTYGQVG
                     TKVEIL"
     gene            complement(4006..4932)
                     /locus_tag="PLG52_03260"
     CDS             complement(4006..4932)
                     /locus_tag="PLG52_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNB40491.1"
                     /translation="MNNRLTNSQAPAQTGGKSPKNGNPAKGPAKKRNLILPIVLVITG
                     CVVFGFAAWSAVTSPVMASILNRGSNSAEFQQVTPGQSFAQVYIAKPTYTPMGLEPVA
                     QQVVVQPTVTFTPTSQVELAVVQPTDPPTIEAAAEVVVQPTATTESVAVVSTGEAVAQ
                     AQIIADTPTSEYVAPPTAVANAPAPVSSPGNGVRWFDVDLSDQRMYAYEGDTLVRTFV
                     VSTGTWQTPTVTGRFKVWIKLKSAPMSGPGYYLPDVPYIMYFYGDYGIHGTYWHNNFG
                     VPMSHGCVNLSIPDAEWAYNFASVGTVVNVHD"
     gene            complement(5018..5695)
                     /locus_tag="PLG52_03265"
     CDS             complement(5018..5695)
                     /locus_tag="PLG52_03265"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNB40492.1"
                     /translation="MTSRITGTIFGLLIFLAGAIAWTVTTSEALAPIPNVTPTYGKPF
                     AVAEILKPTYTPLPPPTLAPTQEPSLTPQALVEAVIVDSPERVNAAAEEQAPVNYSGG
                     KKILVDISEQHMYVYDGEQIVYSFVASTGMNNATRTGTFSVLDKIPNAYGATWNIWMP
                     NWLGIYWAGSLENGIHALPILPGGATLWAGYLGTPISYGCVVLDTYDAQVLYDWAEVG
                     VPVEIQW"
     gene            complement(5692..6543)
                     /locus_tag="PLG52_03270"
     CDS             complement(5692..6543)
                     /locus_tag="PLG52_03270"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNB40493.1"
                     /translation="MESTNEPVSQEPVVQEPAMHETPIRRRQPKPRGINPFLSTLGIA
                     VLIATLFTAWTPNSIFTNNLSDQLRTLLTPQSAIQSGASTPLPQIRIGIVAGHMGNDS
                     GAVCYDDNGEPDLTEADVNLSIATLVQRSLTERGFQVDLLNEFDTRLNGYRAAALVSI
                     HNDSCDYINDEATGFKVAASLETRDVNRAQRLTACLTDRYQNLTGLFFHANSITPDMT
                     QYHAFSEIDPNTITAIIETGFLYLDREILTKQTDVVAEGVTQGILCFVNNESVLPNPV
                     NLPTILP"
     gene            complement(6595..>6971)
                     /locus_tag="PLG52_03275"
     CDS             complement(6595..>6971)
                     /locus_tag="PLG52_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40494.1"
                     /translation="GQDEAREMKREVVSLAPGVNVKLHEAFYSITSLHVRRVPEARIR
                     IHCESLNETLVIAPHDWSNIWVYGMDIFMVGYATYEEMSQRATLLLPNSRTFQYERTH
                     VKNLSLLVSKLKPIQKLWTAVS"
BASE COUNT         1716 a         1640 c         1824 g         1791 t
ORIGIN      
        1 gcggagaacc ttcgtatgta tggcgcgatg gacaaaagcg ccgtttgaac atgattgcaa
       61 agtttgccgc ggaacgcatg caaggcaaag tgcttgaaaa tggctgcggc gtaggcatgt
      121 acgtagaaaa gatgaccgaa cttggggcaa cagtcaccgg gttggaatat gactttgaac
      181 gcgcctgtga tgcccatgtg aattcgccgc gtatcgtcaa cgccgccggg gaattcctcc
      241 cgcttccttc ttcgacgttt gacctgattc tcagccacga agtgatcgaa cacgtacagg
      301 atgaccgctc cgcgattgcc gagatggtac gcgtgatgaa agtgggtggg cgggctgtca
      361 tcttctgccc caaccgcgga taccccgtgg aaacccatgg tattttctgg aatggcaaat
      421 attattttgg taataagctg tttgtaaatt acctacccag agtgtggcgc gacaaactcg
      481 ccccgcatgt gcgcgtgtat tcccgccgtg atatgcaaaa actatttgag ggattacctg
      541 tcaaattcat tgaacgcacc attatctttg gtgcctacga taacatcatc gcccgcttgg
      601 gtgtcttcgg aaaatttatc cgtgctgtgt tgcaatttat ggaagcgaca ccgctaaaga
      661 tattgggttt atcccatctt tgggtggtag agaagatata aaaaactccg aggttcaaca
      721 cctcggagtt tttctttatt catcaccctc atcgccaccg gaagtccccg gctgttccac
      781 tcgtaccact tcaccgtggt aattgataac tatcttgaaa cccatcatcc ctaaataggc
      841 acgcagttct tcaggaaagc ctgttttcat caaggcatcg aattgcttat ctatattctt
      901 tagctgattc gcgatcatcg cctttgagaa gggatcttcc tgcccaagca ttttggcagt
      961 ctgctccgaa aggacatctt cactgccttt catcatgtct gccatttggt tgagcacggc
     1021 gcgatccact tgctcggcag ggatgtgacg tttgaatccg gcatcatcta ttacgtaagc
     1081 aggttcactg ccaatggatg cggcttgtac ggggttgccg tcttcatcta cgatgagacc
     1141 ggcgaagaga ggttggggca ttaagaactc ctctttcttc tttcctcttt tgttttgcaa
     1201 gaaagaagaa agaggaaaga tatcaagaaa ttattgttgg cgaccttcaa ccgcagcaag
     1261 caaaatgcca gcggcaatgc caagacggcg gtcaacctga cctgccgtat ccatgctgaa
     1321 gtcgatgttc agttcatacg tgaagggatt gaaattctgt ttgaaatcgc ctacgcgagt
     1381 ttcgccgaag agcatgtcgt agttctgcgg cacgagattg gtcaagaagc ggcgcagtaa
     1441 agcaagaccc atgctatctt cgaccaaaac accgcgcaca ttgtcgttga catccagcac
     1501 ttcccattca tcgcgcagaa tcgacttaag acctttgcgg cgcatggctc ccactttctg
     1561 acctgtggca ctgtcaatca catcatatgc agccgagaaa tctacgatct ggcgagcttt
     1621 gatcgccaaa acttcttgtg tcttggcttc atcagcatat acgcgaatat cttcgcgtaa
     1681 cttgaacatc ttctgctcgc tgaagagcag caatctgccg gcggtgtcga acacgcggaa
     1741 tttaccagtg agtgcgaaga cctggcgttt gagtaaatat tggttgcctt gaaaaatttg
     1801 attcatctct ctgtcctttt tttgattcta aaaaatttat cgaacgtctt ttacgttcgt
     1861 gccaacctat tcaatgatta ctttcgtgcc ctgaccgggc aggttcacat aatctacgtt
     1921 gtaaggcaca actgggttcg tccaacggta gacccatttt gcgacactgg agggcaaatt
     1981 gacacatcca ttactgcgga ggcgaccata atcattgtgc caataggttc catggaaggc
     2041 atgcccagag ccagtgaaga aggagcacca tggcaccccg ggcaggttgt agatgttggc
     2101 aacggcatcg ccttggtttg tcatatgaat ggatggacct ttatgatagg tgctccattc
     2161 tcctttgggc gtatcggttc catgctgacc acttgcacag cgtacggaga gaacgacctt
     2221 ctcgccctcg aaggcggtca ccatttgcgt ggcaatatcg acatgcagta atttttcttc
     2281 gggcggaact tcgggcgaaa tttgcgttaa ttcttcattg ggaataagcc gcatattata
     2341 ggaaggaatg taaacggttt cttttaaaat cttattatag acctcatacc aaaccccttt
     2401 ttcttcccga ttcacaatca ctttatttac ccaatgcgtt gattcataat aaaggcgatg
     2461 cgaatttttg gcataaaaaa atggggaacg atacgtcaat gtataaggga cagagacttc
     2521 tgccaattgt ccgtctgtgg ggacagagta aacaggtttt tgataatgct cttccacagg
     2581 caggatcggt gccgagtaga caaaaccgcc atccacttca taccaggtgg tattgaattg
     2641 attgccgaag tagtcgcctt gcacttcagc tttcagctca atgacctggt ctttgccgat
     2701 caattcgact ttgttggcgt ttgcaacagg ggcatcgtag atgggcacac cgctgaagat
     2761 ggcgagtcct tttgaggtgg ggggagcggc tagagcacgg tccacgccta attcggagag
     2821 aacagccccg agtgcgccag aggcggaaag tttaaggaag tcccgacggg agagttggtg
     2881 aatggtcata ttttgattgt aaataataag tttgagaggg ggataagaaa aggcgtcttt
     2941 ggtactacaa gatttcaacc ttggtgccga cctgcccgta ggtaaattct ttgttgaccg
     3001 ggacggaggg cgaagaccat aagaaaagcc atttggcagc cgaacagctc atgttgatac
     3061 agccgtgaga gcgtggacga ccataatcat tatgccaaaa tgtgccgtgg aaggagatgc
     3121 cgcttttggt gaggtattgc acccagggca caccgggcag gtcgaagcca ctggcggcga
     3181 tatcaccggc agccatatgg cgtgagggac gtttgtagta ggtgatgaaa ttgccagagg
     3241 gggtggtgta ggttccacta cgcaaacgtg cgccagtaga gacgggtaca ctgaaaacag
     3301 gcacacccat ttcatacgct gtgacgagtt gttgctctaa ctgcacaacg atcttttttt
     3361 ctttcaacgg aacttcgggg gtgatgggtg ccagttcttc gggcgtcatg atgcggatgt
     3421 gatcggcacg aacgtagtac aactcatccc atttatcatc gcggatctga taccagggtt
     3481 gcccatctgt tttgcctgcc accaaggctt taacccaatg ggtcgtttca taataaatac
     3541 gatagccgac ctttgattcg gtgctgggtt ctacgaaggc atctgtatag ggcacacaga
     3601 cttctgccaa taaaccttca atgggaatat ttgcagtata cggctgactt aacattgttc
     3661 ggacgggttg aatgttgccc gatggaacat agccatcggt gccgacttga taccaaatgc
     3721 gattgtgtga gtcgatatct tcggtcactg cggtattggt gatgttgaag attgcatcgc
     3781 gctgaagcag tttgaccttt gttgcttcga gcgtaggttt atcgtacacc catactgtgc
     3841 gaacggtgac gcgcccttgt tgcaggtcga atgggtcatc agaatcaaaa ttgagcggtg
     3901 gcaccatgaa actggctagc cctagcccgc tcaactttag gaaatctctt cttgataaag
     3961 tcttcatttc taaagaacag agtcacacag ggtgacaact caccattaat cgtgcacgtt
     4021 caccacagtg cccactgaag caaagttata cgcccattct gcatcgggaa tgcttaggtt
     4081 cacgcatccg tggctcatgg gcacgccaaa gttgttatgc cagtaggtgc cgtgaattcc
     4141 atagtcgcca tagaaataca taatgtaagg aacatccggc aagtagtaac ccggaccgga
     4201 cattggcgca gattttagct tgatccacac tttgaaacgt ccggttacgg tgggggtttg
     4261 ccaagtgcct gtagaaacca caaaggtccg taccaaggta tcgccttcgt aagcgtacat
     4321 gcgctgatcc gaaaggtcca catcgaacca acgtacccca ttgcccggcg agctgaccgg
     4381 tgctggtgcg tttgcaacag ccgtaggtgg cgcaacatat tctgaagttg gtgtatctgc
     4441 gatgatctga gcttgagcta ctgcttcacc tgtagataca acggcaacag attcagtagt
     4501 agccgtgggt tgcacaacta cttctgccgc cgcttctatt gttggtgggt ctgtaggttg
     4561 cacaaccgcc aattctactt gagaggtggg tgtaaatgtc actgttggtt gcacaacgac
     4621 ttgttgcgct accggttcca gccccatagg ggtatatgtt ggcttggcaa tatacacctg
     4681 cgcaaaagac tgcccgggtg tcacttgctg gaattctgcg ctatttgagc cgcgattcaa
     4741 aatcgatgcc atcacaggcg atgtgactgc cgaccatgcc gcgaagccaa aaacaacaca
     4801 ccctgtaatg accaagacaa tcggcaggat gagattccgc ttctttgccg gacccttcgc
     4861 cgggttgccg ttctttgggc ttttgccacc cgtttgagcg ggcgcttgtg aatttgtgag
     4921 acggttgttc atggggtcac cttttgaggc atcaacgaat aaggacaatt acaaaacaaa
     4981 cgaactatat aagattataa acaaaaagat tccaatttca ccactgaatt tcgacaggca
     5041 cgccgacctc tgcccagtca tacagcactt gtgcatcgta tgtatccaag acgacacaac
     5101 catacgaaat tggagttccc aaatagccag cccagagggt ggctccgccc ggcaggatgg
     5161 gcaaggcatg gatgccattc tctaagctac ccgcccaata aataccaagc cagttgggca
     5221 tccagatatt ccatgtagca ccgtaggcat tggggatttt atcgagcacg ctaaaggttc
     5281 cggtgcgggt ggcattgttc atgcctgtag aagcgacgaa gctgtagacg atttgttccc
     5341 catcatagac gtacatatgc tgttcagaaa tgtcgacgag aatctttttg ccgccactgt
     5401 agttgaccgg tgcttgttct tctgctgctg cgttgactcg ctcgggagag tccacgatca
     5461 cagcttcaac taatgcttgc ggcgtaagtg aaggctcttg ggtaggagca agcgtcggcg
     5521 gggggagagg cgtgtatgtc ggtttgagta tctcagctac ggcaaagggt ttgccatagg
     5581 tcggtgtaac atttggaatc ggagcaagag cttcagaagt tgtaacagtc catgcgatgg
     5641 cgcccgcaag aaaaataagc agcccaaaaa tcgtgccggt gatgcgagag gtcatgggag
     5701 gatggttggc aggttaacag ggttgggtaa aacactttcg ttgttgacga agcacaaaat
     5761 gccttgcgtt acgccttctg ctaccacatc agtttgtttg gtgagaatct cgcggtcgag
     5821 gtataagaac ccggtttcga tgatggcagt gatggtgttc gggtcaattt ctgagaaggc
     5881 gtgatattgg gtcatatcgg gcgtgatgct gttggcatgg aagaaaagcc cggtcaggtt
     5941 ttggtaacgg tctgtgaggc aggcggtgag gcgttgtgca cggtttacat cgcgagtttc
     6001 aagcgaagcc gccactttga acccggtcgc ttcatcgttg atgtaatcgc aagagtcatt
     6061 atgaatggac accaatgctg ccgcacggta gccattcaaa cgtgtgtcga attcattgag
     6121 caagtccact tgaaagccgc gctctgtgag gctgcgctgt acgagggtcg caatcgataa
     6181 gttgacatcc gcttctgtta gatcgggttc gccattgtca tcgtaacaaa cagcccccga
     6241 gtcattgccc atatgcccgg caacaatgcc aatgcgaatt tgcggcaagg gcgtggatgc
     6301 gccgctttga atagctgatt gcggcgtgag caatgtgcgc agttgatcgc tgagattatt
     6361 tgtaaaaata ctgttggggg tccaagcggt gaaaagagtt gcgatcaaga ccgcaatgcc
     6421 cagcgtggaa agaaaagggt taatgccgcg cggtttgggt tgcctgcgtc gaattggagt
     6481 ttcgtgcata gcaggctcct gaacaacagg ttcctgtgag acgggttcgt ttgtagattc
     6541 catagctaaa taatattacc ttattttgct tggatacaaa tgaaaaacag attgctacga
     6601 aaccgccgtc cataactttt ggatgggctt tagcttggag accagcagcg aaaggttttt
     6661 gacatgcgtg cgttcgtact ggaatgtccg cgagttgggc agtaacagtg tggcgcgttg
     6721 actcatctct tcgtatgtgg cgtaacccac cataaaaata tccatgccat agacccaaat
     6781 atttgaccag tcatgcgggg cgattacaag tgtttcgttc aacgattcgc agtggatgcg
     6841 aatccgcgct tcgggcacgc ggcggacatg cagggacgtg atcgaataga acgcctcatg
     6901 caatttgaca ttgacacccg gtgccaggct gacgacttca cgcttcattt cccgcgcctc
     6961 gtcttgtcca t
//
LOCUS       DAOQNJ010000067         6890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5988_length_6890_cov_7.353621, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000067 DAOQNJ010000000
VERSION     DAOQNJ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5988_length_6890_cov_7.353621"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..826
                     /locus_tag="PLG52_03280"
     CDS             74..826
                     /locus_tag="PLG52_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNB40495.1"
                     /translation="MMNRHPRVVAISQARMGSTRLPGKVMKEICGLPLLEHHLSRVSQ
                     SRLVSEVLVATTTLPTDDAISDWCKRQNIRCFRGDEQDVLSRYTGAARAANADVVVRV
                     TSDCPMIDPEVIDSIIERYLTGKGKWDYVSNTRTRSYPRGLDAEVFSRNALEVANDES
                     HDAEEREHVTPFIYWRPERFAIDQLVNEQDLSQYRWTVDTPEDYQLIQLLIGALYLSN
                     PNYRMRDVLNLLDQHPDWLAINEHIEQKSLSR"
     gene            826..1722
                     /locus_tag="PLG52_03285"
     CDS             826..1722
                     /locus_tag="PLG52_03285"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB40496.1"
                     /translation="MPDKNRRILLVGATGMLGQALLPLAKSTGWEVFGLARQEADYCV
                     DIEDVLALWRCLDELQPGVIINAAAITDLNRCESNPGLAYLVNARPVSVFAEYSRQHA
                     AKLVQISTDHFFSGDGDALHDELARVLLLNEYARSKYCAEAFALTAPGSLVVRTNIVG
                     LRGWAGKPTFVEWLLEAFREHQKVTLFDDYFTSSIDVGSFSDALLALISLPLSGVVNL
                     AAHGSFSKLDFALALANRLGFDDLSFVKDSVRSLSGPARAQSLGLNVTKAEKMLGRKL
                     PSLNEVVSVLAQTIQGKYDVVR"
     gene            1706..2791
                     /locus_tag="PLG52_03290"
     CDS             1706..2791
                     /locus_tag="PLG52_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNB40497.1"
                     /translation="MMLYDSAIFINGREISISQPCYFVADIASNHDGDLDRAKLLIKL
                     AKDAGADAVKFQHFKAEKIVSDYGFRSLGGQLSHQAKWGKPVFDVYKQYECNRDWNLE
                     LAETAKAVGIDFMTTPYDVDAVELLDAYLPAYKIGSGDITWPDFIAYVAQRNKPVMIA
                     SGASDISDVIRAVDAVLQNNRQLILMQCNTNYTGSLENFRYINLNVLRSYALKYPGMV
                     LGLSDHSPGHATVLGAVALGARVIEKHLTDDNSRIGPDHGFSMNPQTWNDMVCRTREL
                     EMALGDGVKRVEENEKQTAVLQQRCLRVTKNLAAGTILTPDCVEVLRPAPDGAMRPYR
                     MHEVVGKTLTCAKVAGDAIYPCDIEGC"
     gene            2794..3318
                     /locus_tag="PLG52_03295"
     CDS             2794..3318
                     /locus_tag="PLG52_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40498.1"
                     /translation="MLTGKYVGLRAIEIGDLPDLLQWRNRPQLRRYFREYRELNCSQQ
                     QAWFESKVNNDPATRMFAIVDLTSHRLLGACGLCYIDWVNRSADFSIYIGADELYIDE
                     KFAPDAALVMIRYAWDELGLHRLWSEIYAFDEAKKRFFEQLGFSLDGRHRQTHWAEGG
                     WHDSLFYGLLSVDN"
     gene            3371..4102
                     /locus_tag="PLG52_03300"
     CDS             3371..4102
                     /locus_tag="PLG52_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNB40499.1"
                     /translation="MKIVATIEARMTSSRLPGKVLMPIMGKPMLAYLIERLKRSRVDE
                     IVVATTDRPTDDLIEQLATNLGVGCFRGSEEDVLGRVLLAAKSVGADVIVEITGDCPL
                     IDPDIVDQVLDVYLSQQVDYASNTLKRTYPRGLDVQVFSTNLLDEVNILTSDPIDHEH
                     VSLYIYEHPEKYRLANLESGLSPAQANLRLTVDTIEDFSLIESVYNSLYLDNPNFSMG
                     HVLAYLSDNPDVAKINSEIHQKPVR"
     gene            4106..5080
                     /locus_tag="PLG52_03305"
     CDS             4106..5080
                     /locus_tag="PLG52_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB40500.1"
                     /translation="MIKAALIGCGKIGSSFADDPKVKGVYAHAHAYSRVDGIALAAVC
                     DNDIEKARHCAVRWGGVLAFSDVAELLADVQPEIVSVCTPDETHVEILEKVLLTSSVK
                     AVLVEKPLAVSFDDATRLVELAKQKNIHLAVNYSRRYSRSHQLLRCQLADRIGVVQCV
                     HGYYTKGLLHNGTHWLDLIRFLFGSVTVTSCVAGLSPFPGDPVGDSSPSVTLSLGNGV
                     PVFLVGLDAAQFSIFEMEIIGSKGRVRLLDSGFNFEWQQVTDSQYFSGYTTLAPGISE
                     PADMQDLTLNALTDLLASVQESREPLCSGEDALAAQALVKQARSYLSA"
     gene            5077..6171
                     /locus_tag="PLG52_03310"
     CDS             5077..6171
                     /locus_tag="PLG52_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40501.1"
                     /translation="MNASSKTILCVAGDPGGAAALVPVIQILDARLPLQLAAYKQALA
                     LWRLNSENVCDLGDSPSLEDAARFLMRVQPALIVTATSVNGVDWECYLLQAARQLKIP
                     ALSILDFWSNYSRRFSLKMPLDSLPQQICVMDARAVGEMRAEGFPEEMLLITGQPALD
                     AATEPLPAAQLLAARNVWPAKDRRLLFVSQPLSALYGGPEECLATLGYNEQSVLSLVE
                     SVVTQLVDQGCALSLKILPHPRESAKNFVAAGFAVAKGFSAKVIVQAADIVIGMNSML
                     LLEAAAAGCVVVSVQPNLQRPDSLGINQSGVIVPVYHEAQLLPVLTSLLGDSELYQHQ
                     QKLALKVAGDGKAANRVVGCIERLLASSVK"
     gene            6193..>6890
                     /locus_tag="PLG52_03315"
     CDS             6193..>6890
                     /locus_tag="PLG52_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNB40502.1"
                     /translation="MSELALLGGMPLRPQGLPKYQTLGAEERHAVNEVLDSGVLSQFL
                     GAWGPDFFGGPRVQKLERAWEYHFNVAHAVSVNSATSGLYAAVGAVGVGPGDEVIVSP
                     YTMSASAVAAVVYGAIPVFADIDPDTFCISARTIAEKITPRTKAIIVVDIFGQPSDWD
                     AIMALATKHGLLVIEDAAQAPGARYKGRWAGTLGHIGVYSLNYHKTIHSGEGGVCVTN
                     DPKLAERMQLIRNHA"
BASE COUNT         1600 a         1482 c         1901 g         1907 t
ORIGIN      
        1 gcttcaaata cagctccgac aataatccgt ggttcctgac cctcgacgaa ctgcggcaac
       61 tggatagcta ggtatgatga atcgtcatcc gcgggttgtt gcgatctctc aggctcgtat
      121 ggggtcgaca cggttgcccg gaaaggtaat gaaagagatt tgcggattgc cattactgga
      181 acatcatctg tcgagggtta gccagagccg cttggttagc gaagtgctgg tagcaactac
      241 cacgctgccg acggatgatg ccatctcgga ttggtgcaaa cgacaaaata ttcgttgttt
      301 tcgtggtgat gaacaggacg tattgtcgcg atataccggc gccgcccgcg cggcgaatgc
      361 cgatgtggtg gtcagggtga cctcggattg ccccatgatc gatccggagg tgattgacag
      421 tatcatcgag cgctatctca ccggaaaggg taaatgggac tacgtttcca atacccgcac
      481 cagaagctac cccaggggat tggacgccga ggtgttttcg cgcaacgcgc ttgaggttgc
      541 gaatgatgaa tcgcacgatg ccgaagagcg tgagcacgtt accccattca tttattggcg
      601 ccctgaacgg ttcgctatag atcagctggt gaatgagcag gatttgagcc agtatcgctg
      661 gacagttgac actcccgaag actatcaatt gatccagttg ttgattggcg cgctgtatct
      721 atccaatcca aattatcgga tgagggatgt gttgaatttg ctggatcagc accctgattg
      781 gctcgctatc aacgagcaca ttgagcagaa atcgctatcc cgataatgcc agataaaaac
      841 cgcagaattt tgctggtggg tgcaaccggc atgcttggac aggcactgtt gccgttggcg
      901 aaaagtacag gctgggaggt atttggcctg gctcgtcaag aagccgacta ttgtgtcgat
      961 atagaggatg tgttggccct ctggcgttgc cttgacgaac tgcagccggg agtaatcatc
     1021 aatgccgcag cgattacgga cttgaatcgg tgcgaaagca atccggggtt ggcttatctc
     1081 gtcaatgcac gaccggtttc agtttttgcg gaatactctc gccaacatgc ggcgaagctg
     1141 gtgcagatat caacagatca ttttttctcc ggtgatggtg atgcgctgca tgatgagttg
     1201 gcccgagtat tgttgctgaa tgaatacgca cgcagtaaat attgcgctga agcgtttgct
     1261 ttgaccgcac ctggctcact tgtcgtacgg acgaatatcg ttggtttgcg gggttgggcc
     1321 ggaaaaccaa cgtttgtcga gtggttgctg gaggctttta gagagcatca gaaagttaca
     1381 ttgtttgatg attattttac ctccagcatt gacgttggct cattttcgga cgcattgctt
     1441 gcgttaattt ccttgccctt gtctggtgtc gttaatcttg ctgcacatgg ttcttttagc
     1501 aagctggatt ttgcactggc attggcaaat agattggggt ttgatgacct gtcttttgtc
     1561 aaagattctg tccgatcttt atccgggccg gcaagggctc aaagtctggg cttaaatgta
     1621 acgaaagcgg aaaaaatgct cgggcggaaa ctgcctagcc ttaacgaggt agtttctgtt
     1681 ttggcacaaa ccattcaagg gaaatatgat gttgtacgat agcgctattt tcatcaatgg
     1741 gcgtgagata tcaattagcc agccctgtta ttttgttgca gatatagcct ccaatcatga
     1801 tggcgaccta gatcgcgcca aattgctgat caaactggct aaagatgctg gcgctgatgc
     1861 tgtaaaattt cagcacttca aagcagaaaa aattgtcagc gattacggat tccgctctct
     1921 tggggggcag ttgagccacc aggcgaaatg gggaaaaccc gtatttgatg tttacaaaca
     1981 atacgaatgc aaccgggact ggaatcttga attggcagaa acagccaagg ctgtcggcat
     2041 agatttcatg actacgcctt atgatgttga tgccgtcgag ctgcttgatg cttatttacc
     2101 ggcatacaaa attggttcgg gagatataac ctggccagac tttattgctt atgttgctca
     2161 acgtaataag cctgtcatga ttgcttccgg tgcctcggat atatccgatg tcatcagggc
     2221 tgttgatgcg gttctgcaga ataatagaca gttgatattg atgcagtgca ataccaacta
     2281 cacgggatct ttggagaatt ttcgttatat caatctcaac gtattgagaa gttatgcact
     2341 gaaatatcca ggaatggtgc ttggcctgtc ggaccattct cctggtcacg ctacagtgct
     2401 gggggctgtg gcacttgggg cccgtgttat cgaaaaacat ctcaccgatg ataattcgcg
     2461 gatcggaccg gaccacggtt tttcgatgaa cccacaaact tggaatgata tggtatgccg
     2521 aacccgagag ttagagatgg ctttgggtga tggcgttaaa cgtgtcgaag aaaatgaaaa
     2581 gcaaacggcg gtattgcagc aaagatgcct ccgggtaacg aagaatctag ctgctggaac
     2641 gatattgact cccgactgtg ttgaagtgct gcgtcccgcg ccagacggtg caatgcgacc
     2701 gtatcggatg catgaggttg tgggtaagac tttgacatgc gctaaagtgg ctggcgatgc
     2761 catatatcct tgcgatattg agggctgtta agaatgctca ccgggaaata tgtggggtta
     2821 cgagcaattg aaattggaga tttgcctgat ttattgcaat ggcgtaaccg cccgcagtta
     2881 cgacgttact tccgtgagta tcgagaactc aactgctcgc aacaacaagc ttggtttgaa
     2941 tccaaggtaa ataacgatcc agccacccga atgtttgcaa ttgttgattt gactagccac
     3001 aggttgttag gggcttgtgg cctctgttat atcgactggg tgaatcgtag tgccgatttt
     3061 tccatttata tcggcgccga cgaactatat atcgatgaga agtttgctcc agatgccgca
     3121 ctagtcatga tcagatatgc ttgggacgaa ttggggttgc atagactctg gtcggaaata
     3181 tacgcatttg atgaggcgaa gaagcggttt tttgaacaac tcggcttttc actggatgga
     3241 cgtcaccgcc aaacacattg ggcagaagga ggctggcatg attcattgtt ttatggattg
     3301 ctctccgttg ataattagct catttcggca tgtatggcat ttttttggtg taaattttgc
     3361 ggtataaaga atgaaaattg ttgcaactat tgaggctcgt atgacctcgt ctagattgcc
     3421 aggtaaggtg ctcatgccga ttatgggtaa gcccatgttg gcgtacctga tagagcgatt
     3481 gaaacgaagc cgtgtggacg aaatcgtcgt cgctacaacg gatcgaccca ctgatgatct
     3541 gattgagcag ttggctacta atttaggggt tggttgcttc cgtggtagtg aagaggatgt
     3601 cttgggaagg gttttgttag ctgccaaatc agttggtgct gatgtaattg tagagataac
     3661 gggtgactgc ccgttgattg atccggatat tgttgatcaa gtattggatg tatatttgtc
     3721 gcagcaagtg gattatgcta gtaatacact taagcgaacc tatccacgtg gcttggatgt
     3781 gcaggtgttt tcaaccaatc tactggatga ggttaacatt cttacttctg atccaataga
     3841 tcacgaacat gtctccttgt atatctatga gcacccggaa aaataccggc ttgcaaacct
     3901 ggaaagcggg ttgagtccgg ctcaggctaa tttgagattg actgtagaca ccatagaaga
     3961 tttttcgttg attgagagtg tttacaattc cttatatttg gataacccga atttctccat
     4021 ggggcatgtt ttggcatatc tatcagacaa tccagatgtc gccaaaatca attcggaaat
     4081 tcatcaaaag cctgtccgct agatcatgat taaagctgcc ttgattggct gtgggaaaat
     4141 tggctcaagc tttgctgatg atccaaaggt gaaaggggta tatgctcatg ctcatgccta
     4201 ttcgagagtt gacggtatag cgcttgcagc tgtctgtgat aatgatattg agaaggcgcg
     4261 gcattgtgcg gtgcgatggg gtggggttct ggctttttcc gatgtagctg agttgctggc
     4321 tgatgtacag ccagagattg taagtgtttg tactccggat gaaacgcacg tagagattct
     4381 agaaaaggtg ttgcttactt catcggtaaa agctgttctt gtagaaaagc cattggcggt
     4441 ttcttttgat gatgcgacga gattggtcga attagcaaaa caaaagaata ttcatctggc
     4501 agtaaattac tcgcgtcgat attcccgttc acatcaatta ttgcgatgcc agcttgccga
     4561 tcgtattggt gtggttcagt gcgttcatgg ttattatact aaaggtcttt tgcataatgg
     4621 cacccactgg cttgatttga ttcgcttctt gttcggatct gtaacggtta cttcttgtgt
     4681 tgcaggtctt tcgccatttc ctggagaccc cgtcggcgat tcaagtcctt ctgtgacatt
     4741 gagtttggga aatggggtgc cggttttttt ggttggacta gatgcagctc agttctcgat
     4801 atttgaaatg gaaataattg gctctaaggg ccgagttcga ttattggatt ccggatttaa
     4861 ttttgagtgg cagcaagtca cagatagtca atatttttca ggttacacca ccttggcgcc
     4921 cggcatttcg gagccagcgg atatgcagga tctcacatta aatgctctga ctgatctatt
     4981 ggcgagtgtt caggagtcac gtgagccgct gtgcagtggc gaagatgctt tggctgccca
     5041 ggctttggtg aaacaggccc gcagttactt gtcggcatga atgcttcatc caaaaccatc
     5101 ctgtgcgttg cgggagatcc gggaggtgct gctgctttag ttccggttat ccagatattg
     5161 gacgcacgct tgccattgca gttggctgct tataaacaag cattggcgct gtggcggctt
     5221 aactcggaaa atgtttgcga ccttggggat agcccttcgc tggaggatgc ggctaggttc
     5281 ttaatgcgag ttcagcctgc tttaatcgta acggcaactt cagttaatgg ggttgactgg
     5341 gagtgttatc tgcttcaggc cgcacgccag ttaaaaatac cggcattatc gatccttgat
     5401 ttctggagca attattcgcg acgctttagc cttaagatgc ctctagactc gttgcctcaa
     5461 cagatatgcg tcatggatgc gcgtgcagtt ggagagatgc gcgcagaagg ctttccggaa
     5521 gaaatgttgc tgattactgg tcagcctgca ttggatgccg ctactgagcc tctgcctgct
     5581 gcgcagttac tggcggctcg taatgtttgg cccgccaagg accggcgctt gctctttgtg
     5641 tctcagcctt tgtccgcatt gtatggcgga cctgaagagt gtctggccac ccttggttac
     5701 aacgagcaat ctgttctgtc tttggtcgaa tcggtagtta ctcaacttgt tgatcaagga
     5761 tgcgcgttgt cattgaaaat tttgccgcat cctcgtgagt ctgccaaaaa ttttgttgcc
     5821 gcaggctttg ctgtggcaaa aggttttagc gctaaggtta tagtgcaggc cgccgatatt
     5881 gtcatcggta tgaactcaat gctcttgctt gaggctgcag cggctggctg tgtggttgtg
     5941 agtgtacagc ctaatttgca gcggcccgac agtttgggca taaatcagag tggcgttatc
     6001 gtgcctgtct atcatgaagc acaattgttg ccggtcttaa cttcactgtt gggcgattct
     6061 gaactttatc agcatcagca aaaactggcg ctcaaggttg ccggcgatgg taaggctgca
     6121 aatcgcgtgg ttggttgcat cgagcgtctt ttggcctcct cggtaaaata gaactagata
     6181 gagaaatttg tcatgagcga actcgcgtta ttgggtggaa tgccgttgcg ccctcaggga
     6241 ttgccaaaat atcagacatt gggcgcggaa gagcgccacg cagtaaacga agtgctggac
     6301 agcggtgttc tgtcgcagtt tctcggagcc tggggccctg atttctttgg tggtccgcgt
     6361 gtgcagaaac tggagcgcgc ttgggagtac catttcaatg tcgctcacgc ggtcagtgtc
     6421 aattcggcta catccgggtt gtatgctgcg gtcggggccg taggtgttgg tccaggggat
     6481 gaggtgatcg tctcgcctta taccatgagc gcctcggctg tggctgccgt ggtgtacggt
     6541 gccattccgg tgttcgcgga tattgaccct gatacctttt gcatctcggc acggaccatt
     6601 gccgaaaaaa tcacgccgcg caccaaggcc atcattgttg ttgatatctt tggccagccg
     6661 tctgactggg acgccatcat ggcgctggcg acaaaacacg ggctgctggt gatcgaggat
     6721 gcagcacagg cgccgggcgc gcgctacaag gggcgttggg ccggcacgct ggggcatatc
     6781 ggcgtataca gcctgaatta tcacaagacc attcacagcg gcgaaggcgg cgtttgcgtc
     6841 accaacgacc ccaagctggc ggaacgcatg cagctgatcc gcaaccacgc
//
LOCUS       DAOQNJ010000068         6883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_5995_length_6883_cov_8.270943, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000068 DAOQNJ010000000
VERSION     DAOQNJ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5995_length_6883_cov_8.270943"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLG52_03320"
     CDS             <1..707
                     /locus_tag="PLG52_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40503.1"
                     /translation="LELNNVHSYYGHIHALKGISLHVNEGEIVTLIGSNGAGKSTSLR
                     TISGIIHAREGKVTFDGKDITHVDPHQIVKMGLGHVPEGRGIFPKLTVQENLDMGAYT
                     INDAKSIKDRMEIVYETFPRLKERITQYGGTLSGGEQQMLAIGRGLMLNPRILMLDEP
                     SMGLAPVLVDLIFDTIKKLNEQGTTILLVEQNALMALSIAHRGYVLQTGAIALTDDAA
                     KLKQNEMVQKTYLGIE"
     gene            811..1968
                     /locus_tag="PLG52_03325"
     CDS             811..1968
                     /locus_tag="PLG52_03325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB40504.1"
                     /translation="MTNLPKTADVIIIGGGVMGASAAYHLAKRGVKNILLLEKESHFG
                     TGATGRCAGGVRYQFSTEINVTLSKHSLPMIERFKEELGQDPGYHKYGYLLVATNEQI
                     VKEFEHNVAMQNRLGVQTELLSGDEVRRRLPLMRFDDALAGTFHHKDGTADPNSIVMG
                     YVTAAQKMGVTALTGVEVLGVTVNGGEVRSVKTNLGEVEARIVLNAAGPWSGLIGAMA
                     GVNIPITPLRRQMFTTNPLQEVPEDFPFVIDFAKSLYFHREGEGLLIGMSNQNEKPGF
                     DQNVDEDFELVNLEAAIERMPLIERASRASHWAGLYEVTPDAHPIYGATDVKGFLLCT
                     GFSGHGFMHGPISGKLMSEFIVDGKFSSVDVSMLDLKRFGEGRLINEYNVV"
     gene            2013..3140
                     /locus_tag="PLG52_03330"
     CDS             2013..3140
                     /locus_tag="PLG52_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNB40505.1"
                     /translation="MTSAEIITIGTEILLGEIVDTNTRYIARTLRSLGVDLYRTVTIG
                     DNTDRIAEAIQRSMERANIIITTGGLGPTVDDPTREAVAQAVGVQTEFREDLWQQVVE
                     TIGRYGRTPSENQKRQAYVPQGALGLRNPVGTAPCFIVETETNAVISLPGVPAEMEHV
                     LHESVIPYLQKRFGLNEVIKVRVLHCAGLGEGMIDEKIADLEELSNPTVGLAAHTGVV
                     DVRITAKAKSESEANEMIAKVEADVRQRLGNVVFGADEDKLEEVTLDMLAKRGLTLTA
                     IESGLDGLLARKVPHTASKPDLDPAALMDALRAARADSKADIALGVSVYVNERAAEMA
                     MITAKGEKSHRITYGGPPKSLPRWSMNLALNWLRTTLEEMK"
     gene            3140..4138
                     /locus_tag="PLG52_03335"
     CDS             3140..4138
                     /locus_tag="PLG52_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNB40506.1"
                     /translation="MAKPKPRKKNDTPLVILAWGLNIFGLCALALVGVAYFALNPVSA
                     ATQAVSVNPAYTATMPPTYTPDVLSINLPTITPNPLSTMIVVQSPTPFVLQNAGTLGR
                     ASVATVGYSVSGRPIEVYMFGNGEHEKMIVAGIHGGYEWNTIALADELIQYIFDHPEV
                     IPSDVTLYILRNMNPDGDARDHGIDGRVNDHGVDLNRNFPANWAKTWDRTGCWDLTKT
                     TGGTGPGSEPETKAVMYFLQSHNVETLISYHSAALGVFPGGIPWEADSKQFAKALAKA
                     TNYPYPPIDTGCVYTGTLADYAVELGVTAVDMELSTHKYTDFDQNVKALEVLLNWR"
     gene            4191..6164
                     /locus_tag="PLG52_03340"
     CDS             4191..6164
                     /locus_tag="PLG52_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB40507.1"
                     /translation="MTQTIDTLFINAIVLTMDEKLTQYDPGAVAVNGDSIVAVGSEAE
                     LKKEYSAKEVVDCGGKILMPGLVNAHTHVPMTLVRGLADDLRLDVWLMGYMLPVERQF
                     VSPEFVRLGTLIACAEQIRSGVTTFNDMYFYEDDIAQATADAGMRAVCGESVMKYPAP
                     DAASYDDSLAYAREFIKKWKGHPLIVPAIAPHAPYSTNPEIIRACVDLAKEFDVPLHI
                     HISETAFEVENMRKEQGMPVVPYVKKLGLFEAKVIAAHCVHIDTGEIRTLQHYKAGVS
                     HNPSSNLKLASGFAPVQKMLEDGLNVGIGTDGPASNNDLDMFEEVRLASFVAKAASND
                     PTVLPAATALTMATRLGAQALHIGHLTGSLEVGKRADLILVDTNPLHNTPRFKRDPQN
                     AYAQLVYASKSTDVTDVMVNGKWLMRDHQLLTLNEKELIAQARELATKIDAFLTEREQ
                     SVLSKLIALGGSMEEESFEVQVKVKVEEPNLIVQNVKRPEIEINAYKHYRQFDEYFLF
                     EDPNQGRLRYREDSLINEKGDIVNVRSRLTLLGVKREDEMGEVLLSRSRFLAPAAQSL
                     RFYREYFKPKSEISVEKNRFRWHIKYKNTEFFINLDEVKEPAMGHFLEVKSRTWSRKD
                     ADLKADLVRELLDILGVGNAETVTQDYIEILTA"
     gene            6235..>6883
                     /locus_tag="PLG52_03345"
     CDS             6235..>6883
                     /locus_tag="PLG52_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40508.1"
                     /translation="MEPFRKPIATFFATSFVVTAVMAILFLNFDRRAFSAETYQQAFA
                     REDFYNKIPNLMAQSIVSGANMGQLPTVTQGMSLETWENFIRILLPPEVLKPIGDDVL
                     ISTFAYLNMERNSVQVNLTPVRTSMMSESGSQAILFLLNGLPACNAEQIAQITFNLLS
                     GEQVQLCNPPTDMLPILMPVIQGEMQATAAVIPDQLTLFTAPFQDDPREKLQTARL"
BASE COUNT         1786 a         1817 c         1687 g         1593 t
ORIGIN      
        1 tacttgaact caacaatgtc cattcctatt acggacatat ccatgccttg aaaggcatct
       61 ccctacatgt aaatgaaggc gagatcgtca cgctcatcgg ttccaacggc gcaggcaaga
      121 gcacctctct gcgcaccatc tctggcatca tccatgcccg cgaagggaaa gtaactttcg
      181 acgggaagga catcacccac gttgatcctc atcaaatcgt taagatgggg ctgggacacg
      241 tccccgaagg acgcggtatc ttccctaaat taactgtgca agaaaatttg gatatgggcg
      301 cctacaccat caatgatgcc aagtccatca aagaccgtat ggagatcgtt tacgagacct
      361 tcccccgcct caaggaacgt atcactcaat acggtggtac gctctcgggt ggtgaacagc
      421 agatgctcgc cattgggcgc ggactgatgc tcaacccgcg cattctcatg ctcgacgaac
      481 cgtccatggg tctcgctccg gtgctcgtgg acttgatctt cgacaccatc aagaaattga
      541 atgagcaggg caccaccatt ctgctcgtcg aacagaacgc tttgatggcg ctctctatcg
      601 cccaccgcgg atacgttctg caaaccggcg caatcgctct cacagacgat gcagccaagt
      661 tgaaacagaa cgaaatggtc cagaagacct atctgggtat cgaatagaac gagacaagaa
      721 acaaacgaaa aacccgtttt cacgcgaaag cgggtttttt tgtaaactgc gcacgcttac
      781 aaattgcgct gggtataaaa taaaccaacc atgaccaatc ttcctaaaac agcagacgta
      841 atcattattg gcggcggtgt aatgggtgcc agtgctgcct atcacctcgc aaaacgcggt
      901 gtgaagaaca tcctccttct tgagaaagaa agccacttcg gcaccggtgc gacaggacgt
      961 tgcgcgggcg gggtgcggta ccagttctct acggaaatca atgtgacgtt gtcgaagcac
     1021 agcttaccga tgatcgagcg cttcaaagag gaactcgggc aagatcccgg ttatcacaag
     1081 tatggctatt tgttggtggc aaccaatgaa cagatcgtca aagaatttga acataatgtt
     1141 gccatgcaaa atagacttgg tgtgcagacc gaacttttga gcggtgatga agtccgcagg
     1201 cgtttgccgc tcatgcgttt tgacgatgcg ttggcaggca ccttccatca taaagatggt
     1261 acagcagacc cgaacagcat cgtcatgggg tatgtcactg cggcacagaa gatgggtgtt
     1321 actgcgctca ccggcgtgga ggtacttgga gtcaccgtca acggcggaga ggttcgcagt
     1381 gtaaaaacaa atttgggtga ggttgaggcg cgcatcgtcc ttaatgcagc gggaccgtgg
     1441 tcgggtctta tcggtgccat ggctggagtg aacatcccca tcactcccct acgccggcaa
     1501 atgttcacaa ccaatcccct gcaagaagta ccggaagatt ttccgttcgt gatcgatttt
     1561 gcaaagtcgt tatattttca tcgcgaaggc gaaggcttat tgatcggcat gagcaatcaa
     1621 aatgaaaagc cgggcttcga ccaaaatgtg gatgaagatt ttgagttggt gaatttagaa
     1681 gcagcgattg aacgaatgcc gttgattgaa cgtgccagcc gcgcctcgca ttgggcaggc
     1741 ttatacgaag tgacacccga tgcgcacccg atctatggcg cgacggacgt gaaaggcttt
     1801 ttgctttgca ccgggttttc gggacatggc tttatgcacg gaccgatctc tggcaaattg
     1861 atgagtgagt ttattgtgga tggaaaattc tcaagcgtgg atgtttcgat gcttgacctg
     1921 aaacgcttcg gagaagggcg tttgataaac gagtacaatg tagtttgacc tcacccctgc
     1981 ccctctccta aaagagaggg gggaaattga atatgacctc agcagaaatc attactatcg
     2041 gtacagaaat attactcggc gaaatcgtag atacgaacac tcgctacatc gcgcgcacgc
     2101 ttcgcagcct gggcgtggat ttatatcgca ccgtcaccat tggcgataac acagaccgta
     2161 ttgccgaagc cattcaacgt tcgatggagc gcgctaacat tatcatcacc actggcgggc
     2221 tgggtcccac cgtggatgac cccacgcgtg aagccgttgc acaagccgtt ggcgtgcaaa
     2281 ctgaatttcg tgaagacctg tggcagcaag tggttgaaac tatcggtcgt tatgggcgca
     2341 caccttctga gaatcaaaaa cgtcaggcat atgttccaca aggtgcgctt ggattgcgca
     2401 acccggttgg aacggcacct tgttttattg ttgaaacaga aaccaatgcc gtcatttctt
     2461 tgcccggcgt gcccgccgaa atggaacatg tcctgcatga gtcggtcatt ccgtatttgc
     2521 aaaaacggtt cgggttgaat gaagtgatca aagtccgcgt gttgcattgc gcgggtctgg
     2581 gcgagggcat gatcgatgaa aagatcgcag acctagaaga attgagcaac ccaaccgttg
     2641 ggctggcggc acataccggc gtagtagatg ttcgcatcac ggcaaaagcc aaaagcgagt
     2701 ctgaggcgaa tgaaatgatc gccaaggtcg aagcggatgt gcgtcaacga cttggcaatg
     2761 tagtcttcgg cgctgacgaa gataagctcg aagaagtgac attggatatg ctcgccaaac
     2821 gcggcttgac cctgaccgcc atcgaaagcg gactcgacgg attgctcgcc cgcaaagtgc
     2881 cgcacaccgc ctccaaaccg gaccttgacc ctgccgctct tatggacgct ttacgcgccg
     2941 ctcgagcaga ctcaaaagca gacattgcgc ttggcgtttc tgtttacgtc aatgaacgtg
     3001 ctgctgagat ggcaatgatc accgccaaag gggaaaaatc ccaccgcatc acttacggcg
     3061 gtccgcccaa gagcctgccg cgttggagca tgaatttagc attgaattgg ctgcgcacaa
     3121 cccttgagga aatgaaataa tggcaaagcc caagccacgc aaaaagaatg acacaccgtt
     3181 ggtcatcctt gcatggggct tgaacatctt tgggttatgt gcgcttgcat tggttggagt
     3241 ggcatacttt gcgttgaacc cggtttcggc tgccacacag gctgtttctg ttaatccagc
     3301 ctataccgca accatgccac ccacctacac gccagatgtt ctctcgatta atttaccgac
     3361 cattacccca aaccctcttt caacgatgat cgtggtgcaa tctcccactc ccttcgtgct
     3421 gcaaaacgca gggacgttag ggcgtgccag cgtcgccacg gtgggatatt ccgtttctgg
     3481 cagacccatt gaagtgtata tgtttggcaa tggtgaacac gaaaaaatga tcgtagccgg
     3541 gattcacggc ggctacgaat ggaataccat tgcattggct gatgagttga ttcaatatat
     3601 ctttgatcat ccagaagtga ttccaagtga tgtgaccctg tatattctgc gcaacatgaa
     3661 ccccgatggt gacgctcgcg atcacggcat tgacgggcgt gtcaacgacc acggtgtgga
     3721 cttgaaccgt aacttccccg ccaattgggc aaaaacctgg gatcgcaccg gctgttggga
     3781 cctcaccaaa accaccggcg gaacgggtcc cggctcggag ccggaaacca aagcagtgat
     3841 gtacttcctg caatcacaca atgttgagac gctcatcagt tatcacagcg cggcactggg
     3901 tgtcttcccc ggcggcattc catgggaagc tgattcaaaa cagtttgcca aagcgcttgc
     3961 aaaagcgacc aactatccct acccccccat cgacacgggt tgtgtttaca caggcacctt
     4021 ggcagattac gcagtagagc ttggtgttac agcagtagat atggaacttt ctactcacaa
     4081 gtacaccgac ttcgatcaaa atgtaaaagc tttagaagtg ctcttgaatt ggagatagct
     4141 tctatttcat atcgctcagg gcgataatca aactataaaa aggagattga atgacccaaa
     4201 ctatagatac cctctttatc aatgccatcg tgttgacgat ggacgaaaaa ctcactcaat
     4261 atgaccccgg cgcagtagct gtaaacggcg attccatcgt tgcagttgga tctgaggctg
     4321 aactaaaaaa agaatattct gcaaaagaag ttgttgactg cggcggcaag attctcatgc
     4381 ccggtctggt caacgcgcat acccatgtac ccatgaccct cgtccgcggg cttgcagacg
     4441 acctgcgcct cgacgtttgg ctgatgggct acatgttgcc cgttgaacgt caattcgttt
     4501 cgccagagtt cgtgcgcctt ggaaccctca ttgcctgcgc cgaacaaatc cgtagcggtg
     4561 ttactacctt caacgatatg tacttttatg aagatgatat cgcccaagcc actgccgatg
     4621 caggcatgcg cgcagtatgt ggcgaatcgg ttatgaaata ccccgcgcct gatgccgctt
     4681 cttacgatga ctcgcttgcg tatgcacgtg aattcatcaa aaaatggaaa gggcatccgc
     4741 ttattgttcc ggctattgcg ccacatgccc cctactccac caaccctgaa attatccgtg
     4801 cctgtgtaga cctcgccaaa gagttcgatg tgccgttgca cattcacatt tctgaaaccg
     4861 ccttcgaagt ggaaaacatg cgcaaagaac aaggcatgcc ggtggttccg tatgtcaaaa
     4921 agcttggctt attcgaagcc aaagtcattg ccgcccactg tgtccacatt gataccggtg
     4981 agatccgcac gttacagcat tacaaagccg gtgtttctca taacccatct tcaaatctca
     5041 agctcgcctc cggctttgcc cccgttcaaa aaatgctcga agatggactc aacgtaggca
     5101 tcggtacgga tggtcccgct tcaaacaatg acctcgacat gttcgaagaa gtgcgcctcg
     5161 cctctttcgt cgccaaggct gcttcaaacg acccaaccgt tctccccgcc gccaccgcgt
     5221 tgaccatggc aactcgcctg ggtgctcagg cattgcacat cggtcacctt accggctcgt
     5281 tggaagttgg caaacgagca gacctgattc tcgtggatac caatccgctg cacaacacac
     5341 cgcgcttcaa gcgtgacccg cagaatgcct atgcccagtt ggtatacgcc tccaaatcga
     5401 cagacgttac cgatgtgatg gtcaacggca agtggctgat gcgcgatcat caattgctta
     5461 cgttgaacga aaaagaactt attgctcaag ctcgcgaact cgcaacaaaa atcgacgcct
     5521 tcttgaccga acgcgaacag tctgtgcttt cgaagctcat tgctctcggc ggttcgatgg
     5581 aagaagaatc cttcgaggtg caggttaagg tcaaggtcga agagcccaat ctcatcgtcc
     5641 agaatgtgaa gcgccctgag atcgaaatca acgcctacaa gcattatcgt caattcgatg
     5701 aatacttcct attcgaagat cccaatcaag gacgtttacg ttaccgcgaa gatagcctta
     5761 tcaatgaaaa aggtgacatc gtcaatgtgc gttcacgttt gactctatta ggtgtgaagc
     5821 gcgaagatga aatgggagag gtgttgctct cgcgtagccg cttccttgca cctgccgcac
     5881 agtcgctgcg tttttatcgt gaatacttca aacccaagtc tgagatttcc gttgagaaga
     5941 accgcttccg ctggcacatc aagtataaga acaccgagtt ctttatcaac ctcgatgaag
     6001 tgaaagaacc cgccatggga cacttccttg aagtgaaaag ccgcacctgg agccgaaaag
     6061 acgcagacct caaagcagac ctcgtgcgag aactgcttga tattcttggc gttggcaatg
     6121 ctgaaaccgt cacccaagat tacattgaaa ttttgaccgc gtaatcaata ccaagggcga
     6181 cctgcctgcc atggtgttta tggcatcggg tcgcccttat gaatttaatt aactatggaa
     6241 ccctttagaa aaccaatagc aacattcttt gccacatcgt tcgttgtcac agccgtgatg
     6301 gcgatcttat tcttgaactt tgaccgccgc gctttcagcg ccgaaaccta tcaacaagcg
     6361 tttgcacgcg aagactttta caacaaaatc cccaacctca tggcgcaatc cattgtatcg
     6421 ggtgcaaaca tggggcaatt gccaaccgtc actcaaggca tgagccttga gacttgggag
     6481 aatttcattc gcattctgct accgcccgaa gtcttaaaac cgattggcga tgatgtgtta
     6541 atctccacct ttgcctatct caacatggaa cgcaactcgg tgcaggtaaa cctcacacca
     6601 gtccgcacca gcatgatgag tgaaagtggc tctcaagcga ttttattttt actcaatggc
     6661 ttgcctgctt gcaacgccga acaaattgca caaatcacat tcaacttgct ttctggcgag
     6721 caggtccaac tctgcaaccc gcccacagat atgctgccca tactgatgcc cgtcattcaa
     6781 ggagaaatgc aagcgactgc tgccgtcatc cccgatcaat tgactttatt taccgcgccc
     6841 tttcaagatg acccacgaga aaaactgcaa accgcccggc tct
//
LOCUS       DAOQNJ010000069         6840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6071_length_6840_cov_10.208106, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000069 DAOQNJ010000000
VERSION     DAOQNJ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6071_length_6840_cov_10.208106"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLG52_03350"
     CDS             complement(<1..376)
                     /locus_tag="PLG52_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40509.1"
                     /translation="MNTFLKSRIWTIFLLVSLLIVACGGSATEAPVATEAPATEAAPA
                     GPTYTIIGAVNRDATQAAQAPFVTVDAQKNLLVVWAENSSGGVRQIFASQLNGTAFQP
                     LGSSINIHTNVLADSPSITVAGE"
     gene            complement(437..2704)
                     /locus_tag="PLG52_03355"
     CDS             complement(437..2704)
                     /locus_tag="PLG52_03355"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNB40510.1"
                     /translation="MPSKHSNFIFRVAIVLMLVFISSCGGAPSDSETAPESSSGVIAK
                     NSSYSSPIAISDDDSLIVVANTLNSTVSLINVAGDANTKLTEVQVGEEPRSVVITPNK
                     AFAYVTNQVSGTVSVIDLNSQTKVTDITVGAEPYGIALTPDGTRAYVANSASNTVSII
                     DTAINTVVGTINIPGVQPRGVAITNNNGGTGQQFVYVTQFLSQPTASGGPGLDQGSEG
                     KVFVISTTDDAQIQGVITLAAHDTGFTADRTAFGGTDKEPTFAYPNQMQSIILKNGRG
                     YLPNVAASPEGPVKFNVDTQAFLSVFDVAAKSELAGGTINLHAAVKAQTFTPKLFLAN
                     PWAIAFKHNANEGYVVSAASDVLVKITIDDNGVPTVVSVPADGDTTRVLTIDVHKNPR
                     GIVINNADTRAYVYNFISKDVSVVDLTTSPEVELARVQSADLPAAGSDAAKFLAGNEL
                     FHSSRGEFDEGVAERMSNEGWQACASCHPDGLSDGVVWAFNSGPRKSVPLNGTFSHEN
                     PTGNQRVLNYSAVFDELEDFELNIRNVSGGQGLIIQSGTTDPDPNVKAFDPPNANREQ
                     LHVGGFPAWDAIVAWTQFRVPSPVSPYRGVDPSSELGQQIAQGRDLFTQANCQACHGG
                     PKWSTPQVDFARVSPFPETITAGKPPEAPIGQLSRFLQIVGTFDPANPLEKNNNNQNA
                     LGTLGYNPPSLLSINAFPPYLHNGSCLTLDCVLENQTHREAGGVTGVLDDPANREALV
                     AFLKSIDITTEPITP"
     gene            complement(2866..3507)
                     /locus_tag="PLG52_03360"
     CDS             complement(2866..3507)
                     /locus_tag="PLG52_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB40511.1"
                     /translation="MKAIRVLIVDDHEIVREGLQTLLSEEKEIEVVGTAGDSDEAVSM
                     VKRHKPDVVLMDVILPGVDGIETTRRVVATNPSTRVLMLTSYSDDQRVREAIQAGAIG
                     YLLKDVLKADLLNAIRSAAAGKPALHAEAQQFLMRQLTGSDSAPHTRLTAREFNILQL
                     IAAGKSNKEIALALHLTEGTVKGYVSTIFDKLEVADRTQAALYAVEHGLLKPK"
     gene            complement(3510..5318)
                     /locus_tag="PLG52_03365"
     CDS             complement(3510..5318)
                     /locus_tag="PLG52_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNB40512.1"
                     /translation="MAEQVNLKTPSSSTSTHHEDINDVIRRADILQRVMSTISSGLEL
                     DPLLSNILESAVKLIEATHGTIGLVGDRNGKPIIRTVATHNMPAEELGAEMAPGIGLA
                     GKVLLEKKTVRLDRYGSLEEPTLQGLAEHSVIGIPILWGEEMIGFFGIGVDARRFTDH
                     DVETLEYFAQYAAVAIHNANLFKANQHALEEMHLLYKTSQRIGLSDDVDGVIDAYLDQ
                     VAAGGKYVCNICLYEFDENNERTEVIVKGRWMPETGSERLDEHLPYVQDNLDPILDAG
                     ETLIFSDVRNDSRAPESLGEIQAASGRWALVMIPLMVRNQRIGLVILSHPGVHEWADE
                     ELSPYRAVAAQLAIAIDHRSQQTLLQQRGQQLAVLQERQRLARELHDSVTQLIFSTTL
                     IAQSISPAWKRNPEEGEKRVERLLELSQTALREMRSLLFELKSSEEIQDTELPVTLTG
                     LERIRHYGLLGAIRLLAQDFSADGILVSVDVDEASEKLFSKQTGSRSGKEPMVDESIY
                     RITQEALNNAVKHAKARQIFISISCQGRQLWVSIADDGKGFKSTSIEKQKPEDGSGLG
                     MKTMKERAEALGGKLSVTSAPKQGTTIELTIPLKES"
     gene            5710..6735
                     /locus_tag="PLG52_03370"
     CDS             5710..6735
                     /locus_tag="PLG52_03370"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNB40513.1"
                     /translation="MYICVLTSTPDDDDIPYDPSPYMNGYRWKQHHVEPTEVEKQIKQ
                     LMDDGVDVFVNLCDGTPDDALSGIALVKALEKYNAAFTGADSKFFDPTRDEMKNAAKR
                     VSVPTPSWAFASRAEDAEKIAKRLKFPMLVKPPHGYASVGIRRNSRVTTVDELREQLK
                     LAIAEFGRALIEEFVEGREFTCLIAENTKNPNKPFTFTPVEFIFPEGESFKHYDMKWV
                     DYEKMSVAVVSDPRIDKILREQTTRVFKELGGNGYARCDYRMDADGTIHMLEINPNCG
                     IFYPPHEPGSADFSLLNDPNTDHTKFMKLIISNAQHRQTRIAAERIIKRQQRKRERVP
                     VEQMAYT"
BASE COUNT         1779 a         1680 c         1581 g         1800 t
ORIGIN      
        1 tctcacctgc aacagtaatg ctgggcgaat ctgcaagcac attggtgtgg atattgatgg
       61 aggagcccaa aggttggaag gcagttccat tcaattggct ggcaaagatt tggcgcactc
      121 cacctgaaga attctcagcc caaacgacca gtaaattctt ttgtgcatct actgtaacga
      181 acggagcttg agccgcttgg gttgcatccc ggttgactgc cccaatgatt gtgtatgtcg
      241 gtccggcagg agcggcttca gtggcaggtg cttctgtggc aacaggcgct tctgttgcag
      301 acccgccgca ggctacgatt aataaactta cgagtaagaa gatcgtccat attcttgatt
      361 tcaagaatgt attcataagg gttctccttc agcgagagag agttgtctcc ctctcgctga
      421 aatgtgacga acaaatttac ggggtgatcg gttctgtggt gatatcgatg gatttgagga
      481 aggcaaccaa tgcttcacgg tttgctgggt catccaatac gccggtcacg ccgcccgctt
      541 cgcgatgggt ttgattttcc aagacacaat ccaaggtcag gcaggagccg ttatggagat
      601 aaggcgggaa ggcattgatg ctcaacaaag aaggcgggtt gtaacctaat gttccaagcg
      661 cattctgatt gttgttgttc ttctcaagcg ggttggcagg gtcaaaggtg ccgacgatct
      721 gcaagaagcg tgagagttga ccaattggag cctcgggtgg tttcccggct gtgatggttt
      781 ctgggaaggg cgaaacgcga gcaaaatcca cttgaggcgt ggaccatttg ggtccaccat
      841 ggcaagcctg acagttcgct tgcgtgaaga ggtcacgtcc ctgtgcgatc tgttgaccaa
      901 gctcactgct tggatctacg ccacgataag gggaaacggg ggaaggtaca cggaattgtg
      961 tccacgcaac gatcgcatcc caagcgggga agccgccaac atgcagttgt tcacggttgg
     1021 cattgggtgg gtcaaaggct tttacattcg ggtctgggtc ggtggtaccg ctttgaatga
     1081 tcaagccttg cccgccagag acgttacgaa tgttcaattc aaagtcttcc aactcatcga
     1141 acactgcgga gtaattcaac acacgctgat tgccagtggg gttctcatgg ctaaacgttc
     1201 cattcaaggg cacgctcttg cgcggaccgg aattgaacgc ccacaccaca ccgtcgctca
     1261 aaccatcggg gtgacaactt gcacaagcct gccaaccttc gttggacatt ctttctgcca
     1321 cgccttcgtc aaactcaccg cgtgaagaat ggaataactc attcccagcc aggaattttg
     1381 cggcgtctga acctgcagcg ggcaaatctg ccgactggac acgagccagc tcaacttcgg
     1441 gtgaggtggt gaggtccacc acagagacat ctttggaaat gaagttgtaa acgtatgcgc
     1501 gagtatccgc attgttgatt acgattccgc gcggattttt gtggacatca atcgtcaaga
     1561 cgcgcgttgt atctccatcc gccggcacag aaactaccgt aggcacaccg ttatcgtcga
     1621 tagtgatctt gactaataca tcactcgctg ctgaaacgac atagccttca ttggcattgt
     1681 gcttgaaggc aattgcccat gggttggcaa ggaataactt gggcgtaaag gtttgtgctt
     1741 taacagccgc atgcaaattg atcgtgccgc ctgccaattc agattttgca gctacatcga
     1801 ataccgaaag gaaggcttgt gtatctacat tgaacttcac aggtccttca ggagaagccg
     1861 ctacgttggg taaatagccg cgtccatttt tcaagatgat ggattgcatc tgatttggat
     1921 atgcaaaggt tggttctttg tctgtgccgc caaatgcggt tcgatctgcg gtgaagcctg
     1981 tatcatgcgc tgcgagcgtg atcacgcctt gaatttgtgc atcgtctgtt gtggaaataa
     2041 caaagacttt gccttcactg ccttgatcta aacctggtcc acctgaagcg gtgggttggg
     2101 agaggaactg ggttacataa acgaattgct gcccggttcc tccgttgtta ttggtgatcg
     2161 ctaccccacg tggttgcacg ccgggaatat tgatcgtacc gacaactgta ttgattgctg
     2221 tatcaatgat cgaaaccgta ttcgaggcgg aatttgcaac ataggcacgg gttccatcgg
     2281 gggtgagcgc gatcccgtac ggctcagctc caacggtgat atcagtgacc ttggtctgtg
     2341 aattcaagtc gatgaccgag accgtaccgc tgacctgatt ggtcacataa gcaaaggctt
     2401 tgttcggcgt gatcaccaca gagcgcggct cttcgcctac ttgaacctcg gtcaacttgg
     2461 tgttggcatc gcctgctacg ttgatcagcg acaccgtgct gttaagcgta tttgcaacca
     2521 cgatgagtga gtcatcatca ctaatggcaa tcgggctgga atagctcgaa ttctttgcga
     2581 tcaccccact gcttgactct ggcgcagtct cagaatcaga cggcgccccg ccgcacgaac
     2641 ttatgaatac aagcataagc acaattgcga ctcgaaaaat aaaatttgaa tgtttcgatg
     2701 gcatacttct ctccttctat ataatggata ccgataacgg gatactcata atcatagaag
     2761 aaagcccttt caaaacaaac catcaagagc cattgaatca aaagaaaaag gttagccatt
     2821 acggctaacc aaagttaggt acagaatcag acggaggaag gcgatttatt tcggcttcaa
     2881 tagcccatgt tccaccgcat aaagcgcggc ttgtgttcgg tctgctactt ccaatttatc
     2941 gaaaatcgta ctgacatagc ctttcacagt cccttctgtg aggtgcaaag cgagcgcgat
     3001 ctctttgttg cttttcccgg cggcgatcaa ttgcaagata ttgaactctc tggcggtcaa
     3061 ccttgtatgc ggcgcagaat ctgaaccggt gagttggcgc atcagaaatt gctgcgcttc
     3121 tgcgtgtaag gcaggttttc cggcagcggc agatcgaatc gcatttaata aatctgcctt
     3181 gagcacatct ttcaataaat aaccaatcgc gcctgcttga atggcttctc gcactcgctg
     3241 atcgtcagaa tacgatgtta gcatcaacac ccgagtagat gggttagtgg caacgactcg
     3301 ccgggttgtc tcaataccat ccacacctgg caagatcacg tccataagaa ccacatccgg
     3361 tttatgccgc ttcaccatag agacggcttc atcactatcc cccgcagtgc ccaccacttc
     3421 gatttctttt tcttcgctta gcaaagtctg caatccctcg cggacaattt catggtcatc
     3481 cacaatcaag acacgaatag ctttcatctt taactctcct taagaggtat ggtcaattca
     3541 atggtcgtac cttgtttcgg ggcagatgtc acactgagtt tgccacccaa tgcttctgcc
     3601 cgctctttca tcgtcttcat tcccaacccg ctaccatcct cgggcttttg tttctcaatg
     3661 gatgtacttt tgaagccttt tccatcatct gcaatagaaa cccacaattg cctgccttgg
     3721 caagagattg atataaaaat ctgacgagcc ttggcatgct tgacggcatt gtttagcgcc
     3781 tcttgagtga tgcggtaaat gctttcatcc accatcggct ctttccccga cctcgaacct
     3841 gtctgcttac taaaaagctt ttcactcgcc tcatccacat ccacacttac aagaatacca
     3901 tctgctgaaa aatcttgtgc caacaatcga atggcaccta atagcccata atggcgaata
     3961 cgctccagcc cggtcaatgt gacgggcagc tcagtatctt gaatctcttc acttgatttc
     4021 aattcaaaca gtagcgaacg catctcacgc aacgcagtct ggctcaattc caacaagcgt
     4081 tctactcgct tttcgccttc ctctgggttc cgcttccacg ccggcgaaat tgactgagcg
     4141 ataagagtcg tgctaaagat caactgagtc acagaatcgt gcagttcacg agccagccgc
     4201 tgacgttcct gtagcacagc cagttgctgt ccgcgttgtt gtaacaaggt ctgttgagag
     4261 cgatggtcaa ttgcaattgc caattgtgcc gccactgccc ggtatgggct taactcctcg
     4321 tcagcccatt catgcacccc agggtgactc aaaattacca atccaatccg ttgatttcgc
     4381 accattagcg gaatcatcac caatgcccat ctgccagatg ctgcctgaat ttcaccaagc
     4441 gactctggcg ctcgcgagtc atttctgaca tctgagaaga taagagtctc tcccgcatca
     4501 agaatggggt caaggttatc ttgcacgtag ggcaggtgtt catccaaacg ttctgagccg
     4561 gtttcgggca tccaacgtcc ttttacgatg acttctgtcc gctcgttgtt ttcatcgaat
     4621 tcataaagac aaatattgca gacatattta ccgcccgccg caacttgatc caaataagca
     4681 tcgatcactc catctacatc atcggaaagt ccaatgcgtt gacttgtctt ataaagcagg
     4741 tgcatttctt caagtgcatg ctgatttgcc ttgaaaagat tagcattgtg aatggcaacg
     4801 gcagcgtatt gagcgaaata ctccagagtt tccacatcat gatctgtaaa acgccgagca
     4861 tctacgccaa ttccaaagaa tccgatcatc tcttcgcccc ataaaatggg gattccaatg
     4921 acggaatgtt ctgcaagccc ttgaagagta ggttcttcta gtgagccata acggtctaag
     4981 cgtactgttt tcttttcaag taatactttt ccagctaacc ctatccctgg agccatttct
     5041 gcgcctagtt cttcggctgg catattatga gttgccactg tgcggatgat aggcttgcca
     5101 ttgcggtctc caacaagccc gatcgttcca tgtgtggctt caattagttt gacggcgctc
     5161 tcaaggatgt tggagagtag tgggtcaagc tccaatccgc tactgatcgt tgacatcaca
     5221 cgctgaagaa tatccgcacg gcggattaca tcattgatat cttcgtgatg tgtagaggtg
     5281 gatgaagaag gagttttcaa gtttacttgc tctgccaata agttttacga attatatacc
     5341 aatgaataga ttgatttgaa tgaaattgta gactgaaact tatatgcaga gttggtgtta
     5401 tttacacaag taacaccaaa caaggacatt ctttttctta aggacgttaa taaccttaaa
     5461 aaccttaatg actttatgaa taaacattat aaaaagatga ataaagcttt caaatatttt
     5521 aagataaaac catgtgtaaa aacctttaca taaacaaata gttgtataca ataatcatat
     5581 attgatgata atttaaatat tttttcatca tatgatgata cgtaaaacat ttaaatatta
     5641 tatagatagc atgcgacatg aatataaata aaatcatgaa cgcaaaaaag gaatgacaaa
     5701 ggaaatatca tgtatatctg cgtactaaca agcacacctg atgacgacga catcccatat
     5761 gatccttcac catacatgaa tggatatcgt tggaagcagc atcatgtgga accaaccgaa
     5821 gttgaaaaac aaatcaaaca attgatggat gatggcgtgg atgtattcgt caatttgtgt
     5881 gatggtacac ccgatgatgc actttcagga attgccttgg tcaaggcgct tgaaaaatat
     5941 aatgctgcct ttactggtgc agattcgaaa ttttttgacc ccactcgcga tgaaatgaag
     6001 aacgccgcca agcgagtttc tgttcctaca cccagctggg catttgccag ccgtgcagaa
     6061 gatgcagaga agattgcgaa gcgtctcaaa ttccccatgc ttgtcaagcc gcctcatggc
     6121 tatgctagcg ttggcattcg ccgcaactcg cgcgtcacta ctgtcgatga gcttcgtgaa
     6181 caattgaaac ttgccatcgc tgagtttgga cgcgccctta ttgaagaatt tgtggaagga
     6241 cgcgagttta cctgtctcat tgcagagaac accaagaacc cgaacaagcc ttttacattc
     6301 actccggtag aattcatctt ccccgaaggc gaatctttca aacactatga tatgaaatgg
     6361 gtcgattacg aaaagatgtc tgtagctgtg gtgagcgatc cccgcatcga caagatcttg
     6421 cgtgaacaaa ccactcgggt cttcaaagaa ttgggtggca acggttatgc tcgctgtgat
     6481 tatcgtatgg atgcagatgg cactattcac atgctcgaga tcaaccccaa ctgcggtatc
     6541 ttctacccgc ctcatgaacc gggttctgcc gatttctcct tgctcaatga cccgaacacc
     6601 gatcacacca agtttatgaa gttgatcatt agcaatgccc agcatcgtca gactcgcatt
     6661 gccgctgagc gcatcatcaa gcgccagcaa cgcaagcgcg aacgcgtgcc ggtcgaacaa
     6721 atggcttaca cctaagaaaa acgggacaca ttgaagtgtc ccgttttttt atccttgaag
     6781 gatacttgct ccttcactgg ctttgcagac gtaaatactc gcctccaacc cggacctttg
//
LOCUS       DAOQNJ010000070         6782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6153_length_6782_cov_7.205441, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000070 DAOQNJ010000000
VERSION     DAOQNJ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6153_length_6782_cov_7.205441"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="PLG52_03375"
     CDS             <1..1353
                     /locus_tag="PLG52_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40514.1"
                     /translation="PVATEAPATEVAPTGPTYTIIGAVNRDATQAAQAPFVTVDAQKN
                     LLVVWAENSSGGVRQIFASQLNGTAFQPLGSSINIHTNVLADSPSITVAGENNAVPWV
                     AWSEPSPGFDNVAQVFASRFSVETGLWQQAGTDRGGGEASLNISTERAGTHPFIFSGS
                     TEIGNPPVPWVAWEEVSDVNPSSVQIFVAKGVKDESAIGGFRWETVGQVKEDGEQTLN
                     VNRFRISQHPTGVFAETSNSVPWVTWQEQGRDEPSRIFTARGVADPNTPGGFKWINVP
                     PCDPKDETKCALNINPLNDAKDASMASGSTVPGEGNVPWIAWPEIGQNGKWQIFVSRL
                     DTSTRNSFLQVGGSLNIDPNHDAKLPTIVFVGNVPYVAWLEDDGTGKFEVQMRHLASD
                     PQTGTWALDTPAEGFNKDKTLSDFNLSVVASTDTVFLAWGEGDPATSASQMVIGAFRP
                     "
     gene            1375..1785
                     /locus_tag="PLG52_03380"
     CDS             1375..1785
                     /locus_tag="PLG52_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB40515.1"
                     /translation="MPLRLLMHPAHPARDSYRILIVDDAPTVRESLKWLLEDESGLTV
                     IGDAGNGDDAIQMAVNLKPDLVLLDIELPDMDGFAVAKQIKALLNPPRIILLSMHNDA
                     VSKKRGAEAGCDAFVGKEFGWGVLLPVLRTVLES"
     gene            complement(1825..2685)
                     /locus_tag="PLG52_03385"
     CDS             complement(1825..2685)
                     /locus_tag="PLG52_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB40516.1"
                     /translation="MSTLTLASEVAMKRLLILTVGLLVLTACASPTPTPVPDAVETTA
                     PVIEVSSPDVVIASADVEPTHVSHLGFTISALVKEVAVGEGDVVKAGDTLMVLNTPDL
                     QYAVVSAEADFNARTQAAQLQKADKVLYVNPNTGKKTWYSLPREVFLKAQAQADQAKA
                     VWDTAAATYAQSTLTAPFDGVVVDVAVIPGELVQVNQVVITIADLSDLQITTTDLSER
                     DIARVKLGQSVNVSIEALGQTISGKVIRISPVSETVGGDVVYPVTIQLDEQPDGLMWG
                     MSAEVEISTK"
     gene            complement(2660..3493)
                     /locus_tag="PLG52_03390"
     CDS             complement(2660..3493)
                     /locus_tag="PLG52_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB40517.1"
                     /translation="MKRVIFVVVSLLALLLSACGQASSTEAVPTVVIDAGNSSQPADS
                     QPSSGGSVSASAIVVPMQDANLSFTNIGRVTQVNVQVGDKVSAGDVLVQLDTTILEAK
                     VREAEANLKYAQISLDYLIRNVGCKMNCAPSQEHIEVAENDVAKAQALVDSAKAVLAA
                     QSNLTAPFAGTVISVDIASYETVAPGQVVMVIGDLSKYQIETTDLSERDVTQVKVGQP
                     ASVYIEALDEEFTGKVVDVARISSEVGGDVVYKVTIEFDKQPAGLLWGMSADVNIDAG
                     E"
     gene            complement(3582..4265)
                     /locus_tag="PLG52_03395"
     CDS             complement(3582..4265)
                     /locus_tag="PLG52_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40518.1"
                     /translation="MEVVKIENITRTYQIGKLETHALRGVSLSINSGEFTALVGPSGS
                     GKTTLLQMIGCLDQPTSGRVTVDGKEVTSLNRNQRADMRKGHIGFIFQFFALIPTLTA
                     YENVEMPLLLNGHSPKERRDRVMELLKAVDLTDRANNRPDQLSGGQQQRIAIARALAP
                     KPALILADEPTANLDTENGKQVMEIMKKLNQETGETFVFATHDPRVINYASRVVTLED
                     GVIVKDSKG"
     gene            complement(4295..5515)
                     /locus_tag="PLG52_03400"
     CDS             complement(4295..5515)
                     /locus_tag="PLG52_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40519.1"
                     /translation="MIQIFKMAFRDLGRNRRRNFFSALAVAIGLALLMLMSSVIQGEM
                     GSAVESAINLQSGHIQIRAASYDANKSSLKWEDLVANPDEIAAQISALEPVKAATPRL
                     YASGFLSTGDESTGVQINGIDPQSVANDPYRNGIISGEFLNADDREGLLIGRPAAEKL
                     HLKVGDKVSLSVNTADGNVQEQVFIIRGIYTTDTYGFDSGTVFLPLAKAQAITQTEGH
                     ASTIFVLLKDTADTDQVVAALKSSSSLEVLTWRDLNKLFVEFETMAQSYISFFYLIIL
                     AITASVIVNTLIMSVYERTREIGILSAIGMRGSRILGLFLAESFMLAVGGIVMGLALG
                     VFAVYLFNINGFFVGNMGLSGMLVSDTIRAQLTVDNTVNVTVMTFIATILSGLYPAVM
                     ASRLEPVQALRAEK"
     gene            complement(5524..6750)
                     /locus_tag="PLG52_03405"
     CDS             complement(5524..6750)
                     /locus_tag="PLG52_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB40520.1"
                     /translation="MNLYLRLAWRNIWRHKRRTVIIVLAMSMSLSLMMFYDGLMNGFT
                     DAIYGNAVKVLGGNIQIHAEGYRAEVGSTPLLPLTDAQAVVNAAEADTNVVAATQRIN
                     TGGLVTNREGAFAVVITGIEPEKEVQVNLIGQYVTEGRNLVSDDGDNILIGKGLADAM
                     DVTVGDRITLVGRSQNKEMRQRTMTIVGVFDLGMADIEKQTVYISLNEAQALYELSGP
                     TEVAIFLEHLGQEEQVITSLQASLPGYEIESFQANYPDLANAIDSKGGVMNVFSVIII
                     AIAGVGILNLLLMAVYERTREIGVLGAMGLKANQIALLFILEGTMIGLVGVAVGIALG
                     LALNGYLMKVGMDFGSLTQAASYMALIKDKVYPTLGVEKLFMRASMVAVISALASVIP
                     AIEAGRREPAEALHFV"
BASE COUNT         1683 a         1806 c         1686 g         1607 t
ORIGIN      
        1 cctgttgcca ccgaagcccc agctacagaa gttgccccca ccggacccac ctacaccatt
       61 attggtgctg ttaaccggga tgcaacccaa gcggctcaag ctccattcgt aacagtggat
      121 gcgcaaaaga atctactggt tgtttgggct gagaattcct ctggcggagt acgccagatc
      181 tttgccagcc aattgaatgg aactgccttt caacctttgg gctcctccat taatatccac
      241 accaatgtgc ttgcagattc gcccagcatt actgttgcag gtgagaacaa tgccgtgcct
      301 tgggtggcgt ggtctgagcc cagccccggt ttcgacaatg tggcgcaggt gtttgcgagt
      361 cgtttcagtg tagagacagg tttatggcag caagcaggca cagacagagg aggtggcgag
      421 gcgagtctca acatctcgac agagcgcgca ggcacgcatc catttatttt ctcagggtcc
      481 acagagatcg ggaatccgcc cgttccgtgg gtggcatggg aagaagtctc ggatgtgaac
      541 ccatcttctg tgcaaatttt tgtagccaaa ggcgtaaagg atgaaagtgc gattggcggc
      601 ttccgatggg agaccgttgg gcaagtgaaa gaagatggcg aacagacttt gaatgtcaat
      661 cgcttccgca tctcacagca ccctacgggc gtgtttgctg aaacgtcgaa ctcggtaccg
      721 tgggtaacct ggcaagaaca gggacgtgat gaacccagcc gcatttttac ggcacgtggt
      781 gtggcagacc ccaacacgcc cggcggtttc aagtggatca atgttccgcc ttgtgatccg
      841 aaagatgaaa ccaaatgcgc gctcaacatc aatcctttga atgacgcgaa agatgcttcc
      901 atggcttctg gcagcactgt tcccggtgaa ggcaatgtgc cgtggatcgc atggcccgaa
      961 attggtcaga atggcaaatg gcagatcttt gtctcgcgtt tggataccag cacgcgcaat
     1021 agttttcttc aggttggcgg cagtttgaac attgacccga atcatgatgc caagttaccg
     1081 accattgtat ttgttggaaa tgttccgtat gtggcttggt tggaagatga cggcacaggc
     1141 aaatttgaag tgcagatgag acacctcgcc tcagacccac aaaccggaac ttgggcgctc
     1201 gataccccag ccgaaggctt caataaagat aaaacccttt cagatttcaa tctctctgtg
     1261 gttgcttcta cagatacggt cttccttgct tggggcgaag gtgaccctgc aacctctgcc
     1321 tcgcaaatgg ttatcggcgc attccgcccg taacgagctg atatgcagaa ctccatgccc
     1381 ctgaggcttc tcatgcaccc tgcccaccct gcaagagata gttaccgaat tctgatcgta
     1441 gatgatgcgc ctaccgtacg tgaaagtttg aagtggttgt tggaggatga atcgggactg
     1501 accgttattg gcgatgccgg gaatggcgac gatgccattc aaatggcggt caaccttaag
     1561 cctgatctgg ttctgctcga tatcgaatta ccagatatgg acggctttgc cgttgcgaaa
     1621 cagatcaaag ccctcctcaa cccgccgcgg atcattctgc tttccatgca taacgatgcg
     1681 gtctctaaga aacgaggtgc agaggcaggc tgtgatgctt ttgtaggaaa agaattcggt
     1741 tggggtgtgt tgttacctgt attaaggaca gttttggaaa gttgatacaa aaaaggaggt
     1801 cacgttttga acgtgacctc ctttttattt cgtagaaatt tctacttcag cgctcattcc
     1861 ccacatcaag ccatcgggtt gttcatccaa ttggatagtc accgggtaga ccacgtcacc
     1921 gcctacggtt tcagaaaccg gtgaaatgcg aatgactttt cctgaaatgg tctgccccaa
     1981 cgcttcgatg gaaacgttca cgctctgccc gagttttaca cgcgcaatat cgcgttcgct
     2041 caagtctgtg gtggtgattt ggaggtcgct caagtctgct atggtgataa cgacttgatt
     2101 cacctgcacc aactcacccg ggatgaccgc aacatccaca accacgccat caaagggagc
     2161 tgttagggtg gattgagcat atgtagcagc ggcagtgtcc caaacggctt ttgcttggtc
     2221 ggcttgggct tgggctttca agaacacttc acggggcaag ctataccaag ttttcttgcc
     2281 ggtgttcggg ttgacataca aaaccttatc ggctttctga agctgcgctg cctgcgtgcg
     2341 cgcattgaaa tctgcttcag cagaaaccac ggcatactga aggtctgggg tattcaagac
     2401 catcaacgta tcgccagcct taaccacatc cccttcacca acggcaactt ctttaacaag
     2461 tgccgagata gtaaacccaa ggtgagaaac gtgagtcggt tcgacatcag cagaagcaat
     2521 caccacatct ggcgaagaca cttcaatgac cggggcagtt gtttcaacgg catcaggaac
     2581 aggcgttggg gttgggctgg cgcaagccgt tagcactagc aagccaacgg taaggattaa
     2641 caatcgcttc atggcaacct cactcgccag cgtcaatgtt gacatccgcg ctcatgcccc
     2701 acaataagcc tgcaggctgt ttatcaaatt caatggtcac tttgtagacc acgtcgccgc
     2761 caacctctga agagatgcgt gccacgtcca caactttgcc agtgaattct tcgtccaacg
     2821 cttcgatgta taccgaagca ggctgaccca cttttacctg agtcacatcg cgttcgctca
     2881 agtcggtagt ttcgatctga tacttggaga ggtcaccgat caccattaca acctgtcccg
     2941 gagcgaccgt ctcatacgag gcgatatcga ccgagataac ggtaccagca aacggcgcgg
     3001 tgagattcga ttgagcggcg agaacagcct tggctgaatc caccaaagct tgagccttgg
     3061 caacgtcatt ctctgctact tcaatgtgtt cctgtgaagg tgcacaattc atcttacagc
     3121 ccacattgcg aatcaaataa tcgaggctga tctgcgcgta tttcaaattg gcttctgcct
     3181 cgcgaacctt ggcttccaaa atggtggtat ccaactgaac caacacatca cctgcgctaa
     3241 ccttatcgcc aacctgcaca ttgacctgag tcacacgtcc aatattggta aaggaaaggt
     3301 tggcatcttg catgggaaca acaatcgccg aagcagaaac tgaaccgccg cttgaaggtt
     3361 gagaatcggc aggctgggat gaattgcccg catcgatcac aacggtggga acggcttctg
     3421 ttgaactagc ctgtccacac gcggacagca aaagagccag caaactaaca actacaaaaa
     3481 taactcgttt cattctttat ttctccaaac tgtgtaatcc ttgaagtttc taaagtatat
     3541 catattgcac agtttttgca aaggttttgt caagaaagga cttatccctt agaatccttg
     3601 acaatcaccc catcttccaa agtcaccaca cgtgaagcgt agttaatgac acgcggatca
     3661 tgtgtagcga agacaaaggt ctcccctgtt tcttgattca gcttcttcat gatctccatc
     3721 acctgcttgc cattctcggt atcgagatta gcagtcggct catcggcaag gatgagcgca
     3781 ggtttgggcg ccaaagcacg cgcaatggcg atgcgttgtt gctgaccgcc agagagttga
     3841 tcagggcgat tattggcacg atcggttaga tccacggctt tgagtaattc catcacacgg
     3901 tcgcggcgtt cttttggaga atgcccattg agcagaagcg gcatttccac attttcatag
     3961 gcagtgaggg tggggatcaa cgcaaagaat tggaagataa acccaatatg acctttgcgc
     4021 atgtcggcac gctgattgcg gttcagcgaa gtcacttcct tgccgtccac ggtgacacgc
     4081 ccgctggtgg gctggtcgag acagccgatc atttgcaaga gtgtggtctt gcccgaacct
     4141 gaaggtccaa ccagggctgt gaattctccg ctgttaatgg aaaggctgac gccacgcaag
     4201 gcatgggttt ccagtttccc aatttgatag gtacgagtga tattttcgat ctttacgact
     4261 tccatgatat ttctcctcgc ggattcttcc gcgtttattt ttctgcacgc aacgcctgaa
     4321 ccggctccaa tcgagatgcc atcaccgccg gatacaaccc ggaaaggatg gtggcaataa
     4381 aggtcatgac ggttacattg accgtgttat ccactgtcaa ctgggcacgg atggtatctg
     4441 agaccaacat accgctcagc cccatgttgc caacgaaaaa gccattgata ttgaagagat
     4501 agacggcaaa cacgcccaat gccaatccca ttacgatgcc gcctactgcc agcatgaacg
     4561 attccgccag gaacaatccc aaaatacgac tgccgcgcat gccaatagca gaaaggatgc
     4621 cgatttcgcg cgtccgctcg tagacagaca tgatgagcgt gttgacaatg accgaggcgg
     4681 taatggcaag gatgatgaga tagaagaacg agatatagct ttgcgccatg gtctcgaact
     4741 ctacaaagag tttgttcaag tctcgccagg tgagcacttc caaactcgac gaagatttca
     4801 aggcagccac gacctgatcg gtatccgccg tgtcttttag aagcacaaag atcgtgctgg
     4861 catgaccttc cgtctgtgtg atcgcctgcg cctttgccaa cggcaggaag accgtgccgc
     4921 tatcaaagcc ataggtatcg gtggtgtaaa taccgcggat gataaagacc tgctcctgca
     4981 cattgccatc ggcagtgttg accgaaaggc tgaccttgtc gcccactttc aggtgcaact
     5041 tctctgccgc cggtcttccg atcaataaac cttcacggtc atccgcattg aggaattctc
     5101 cgctgatgat cccgttgcga tatgggtcat tcgccacaga ctgcgggtcg atgccgttga
     5161 tctgtacgcc tgtcgattca tcgcccgtgg aaagaaatcc gctggcatac aaacgcggcg
     5221 tggcagcctt caccggctcc aatgcgctga tctgtgcggc aatctcatcc ggattcgcta
     5281 cgagatcttc ccacttaaga ctcgatttgt tcgcgtcata agaagcggca cgaatttgaa
     5341 tatgtccgct ttgcaggttg atggcagact caacggcact gcccatttca ccttgaatga
     5401 cggatgacat taacatcaac aacgcgagcc cgatcgcaac agccaaggca gagaaaaagt
     5461 ttctgcggcg gttacgaccc aggtcacgaa atgccatttt aaaaatctgt atcatgatgc
     5521 accttataca aaatgcagag cttcagccgg ttcgcgtcgc cccgcctcga tggcagggat
     5581 aaccgatgcc aacgccgaga taaccgcgac catggatgcc cgcatgaaca acttttcaac
     5641 gcccaacgtg gggtagacct tgtctttgat cagcgccata taactggcgg cttgtgtcag
     5701 agaaccaaag tccatgccaa ctttcatcaa atatccgttg agagcaagtc ccagggcaat
     5761 gcctactgcc acccctacca gaccgatcat ggtgccttcg aggatgaaca acaacgcgat
     5821 ctgattggct ttgagcccca tggcacccag cacgccgatc tcgcgcgtcc gttcgtagac
     5881 tgccatcagc aacaggttga gaatgcccac acctgcaatc gcaatgatga tgacgctgaa
     5941 gacattcatc acgccgcctt tggagtcgat cgcattcgca aggtcggggt aattcgcctg
     6001 gaatgattcg atctcatacc ccggcaagct ggcttgcaaa ctggtgatga cctgctcttc
     6061 ctgccccaaa tgctcaagga agatcgccac ttcggtcgga ccggacaatt catagagagc
     6121 ttgtgcctcg ttcaatgaaa tgtagaccgt ctgtttttca atatctgcca taccaaggtc
     6181 aaacacgccg acaatggtca tcgtgcgttg acgcatttct ttgttctgag aacgtccaac
     6241 caaggtgatc cggtcgccca ctgtgacatc catcgcatcg gcgagtcctt tgccgatcag
     6301 aatgttgtca ccatcgtcac tgacaagatt ccgtccctca gtgacatatt gaccgatgag
     6361 attaacctgt acttccttct ccggctcaat gcccgtaatg acaaccgcaa aggcaccttc
     6421 acgatttgtc accaaaccgc cggtattgat gcgttgagtc gcggcaacca cgtttgtatc
     6481 tgcttcggcg gcgttgacca ccgcctgtgc atcggtcaac ggcagcagcg gagtggagcc
     6541 cacttctgcc cgatacccct ccgcgtggat ctgaatgttg ccgcccaaga ccttgaccgc
     6601 attgccgtag atcgcatcgg tgaagccatt catcaatcca tcatagaaca tcatcaacga
     6661 caaagacatc gacatggcaa gtacgatgat gactgtgcgg cgtttatgcc gccaaatgtt
     6721 gcgccatgca aggcgcaggt agagattcat agataaactc ctttcgagtg aacgttcaaa
     6781 at
//
LOCUS       DAOQNJ010000071         6735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6237_length_6735_cov_10.525898, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000071 DAOQNJ010000000
VERSION     DAOQNJ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6237_length_6735_cov_10.525898"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /gene="trpS"
                     /locus_tag="PLG52_03410"
     CDS             <1..932
                     /gene="trpS"
                     /locus_tag="PLG52_03410"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNB40521.1"
                     /translation="GNYLGAIENYVGLQTDYDCVYCIVDVHALTTVETTQELRQNTFE
                     MALDWLAAGIRPEDSILFVQSHVPEVMELHTYLSMVTPLGKLTDLPTFKEKVRQQPEN
                     VNYGLLGYPVLMTADIVLYKTDIVPVGIDQAPHIEFARETVRSFNYRFGTKALIEPQV
                     KHTEVKKVVGIDGKDKMSKSLNNHIELALTAEETTKRVREMVTDPARQRKTDPGNPDV
                     CNVFTMHKIFSPQEEVDMINTECRKAGIGCVDCKLRFASNLNKHLEPFRAKRADFASK
                     PAEVQEILNDGGKRARAIAQETMSEVREAMKLP"
     gene            970..1407
                     /gene="dtd"
                     /locus_tag="PLG52_03415"
     CDS             970..1407
                     /gene="dtd"
                     /locus_tag="PLG52_03415"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNB40522.1"
                     /translation="MRVLIQRVSQASVTVEQQTISSIGKGLLILLGIGHGDGEEQVQF
                     LAEKTANLRIFEDEQGKTNLSVLDVKGEAIVVSQFTLYADSRKGRRPSFIDAALPDVA
                     EPLVHRFVELLRSHGVPTQTGKFGAHMQVEIHNDGPVTIWLEK"
     gene            1407..1892
                     /locus_tag="PLG52_03420"
     CDS             1407..1892
                     /locus_tag="PLG52_03420"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNB40523.1"
                     /translation="MKRPNFIQKFVHRFFMLKPVTAFFATRTHIMDGIALKLTGGKYT
                     VSEILGWNIAQIITTGAKTGEKHTIILIAVINGEKIALIGSNFGRKPNPGWYYNLKKH
                     PECEVHLNGKAGMYLARETEGEERESYWKQAVALYAGYEKYKERASHRRIPVMLLEPI
                     K"
     gene            2065..2274
                     /locus_tag="PLG52_03425"
     CDS             2065..2274
                     /locus_tag="PLG52_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNB40524.1"
                     /translation="MAERIIGTVKWFNGTKGYGFIARDGGPDVFVHFSAIRGEGFKNL
                     QEGQKVEFTIEKGPKGLQAADVVVL"
     gene            complement(2362..3114)
                     /locus_tag="PLG52_03430"
     CDS             complement(2362..3114)
                     /locus_tag="PLG52_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40525.1"
                     /translation="MKRDLYFSKPLLNAAGSLGFTPDPRNGISLDSFGAFVTNPLSLR
                     PRLPTTHPALVEFPGGFLMHTGLPNPGFHATLKKYSAKWNRANLPIIVHLMADRPEET
                     QRMVRELENVENVMAVQLGFAPLLSDDIILMNLEMCLGEVPLIFALPHEQVLTLGRQL
                     VDGGASAISIASPRGALYDKNGNLITGRMYGQSLFPRALDMVRSAVMSGIPIIGSGGV
                     WTEMDVESMLIAGAIAVETDAQLWVPKEAPSP"
     gene            3125..4309
                     /locus_tag="PLG52_03435"
     CDS             3125..4309
                     /locus_tag="PLG52_03435"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNB40526.1"
                     /translation="MQPLQNIRVLDLSRVLAGPYCTMVLGDLGAEVIKVEPPEGDETR
                     GWGPPFAGGESAYYLCVNRNKRGMVVNLKTEEGRDVLKKLAMQSDVFVENFRPGTLKK
                     FGLDFETLHELNPKLIYCSISGFGQTGPLKDMPGYDFMIQAMGGIMSVTGEPEGEPMK
                     VGVAVADLFAGQNAIISILAALQARTFTGEGQYIDIALFDSELGWLANVASNFLISGK
                     NPKRYGNAHANIVPYQSFQASDGWFVVAVGNDKQFESFCNVIETPGLSSDERFSTNSA
                     RVQNREVLIPLLKPIFMQKTVNEWLALIGDQFPCGPINTFDQVFSMPHVKEREMLIEM
                     EHPTIGALPLVGSPLKMGGTPVSYRLPPPLMGQHTHEILKEVLEFSDEAVQKLMNDGC
                     IK"
     gene            4327..4839
                     /locus_tag="PLG52_03440"
     CDS             4327..4839
                     /locus_tag="PLG52_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40527.1"
                     /translation="MEVIYSVSPLKAFSGSFLFLAFFFVLGGFGALSAIFSRRDRAGK
                     RIALGCASAIILLAGAGGAVATFMTYTGGQTTALVHVDKKRIVERNCDNASNTCIDYV
                     IEASDGQKYYTFGLAKDTWDVVQDQACYEFTYYPAKSLFGEYLQQDNPYADMYETTSN
                     IVEIKTAQCP"
     gene            complement(4847..5419)
                     /locus_tag="PLG52_03445"
     CDS             complement(4847..5419)
                     /locus_tag="PLG52_03445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB40528.1"
                     /translation="MKLSAPEFNAMQSLPRKLGQRYFEMPLFKRMGLNLQNKDVLEIG
                     CGSGYGAYLLSTLNPKSYIGLDVMEEQVALAKKQYPQFQFILQGAEDLSVFADASKDV
                     VIIFGVLHHIPDWRKAIDEIIRVLKPGGSLFLEEPRGVDIKVFDAFFRWGHPNTDFGL
                     NALDAHLTQRGLTIETKQWTPLLTMYHIKK"
     gene            complement(5461..>6735)
                     /locus_tag="PLG52_03450"
     CDS             complement(5461..>6735)
                     /locus_tag="PLG52_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB40529.1"
                     /translation="GPAGLFGARELANSGVRVALFNRDIKAGGLAEYGIYLEKHTMKE
                     GLRKQFRQALDMPNIDYYGNVAIGTKGDFTLDEIRALGFDAVLVAAGAQGTKSLGLPG
                     EELEGVYHAKDVVYGYNKLPPFSQRKFRYGKRAAIIGAGNVMVDIARYLINHQGVEEV
                     TAVVRRGPAEVNFTKEEMKHLVAYLDMDEFEKELARVRPALDAIGQDPQIGRQKVLDA
                     LAKADPKVKEAKFKFDFLSSPSGMFGENGLLTQLEVEENILTERDGKTSAKGTGKKHT
                     LDVETVIFAIGDQVDANFGLPTEWGEFSKNKEPKFPIEGTSYESTTEGIFVGGWSRKA
                     SEGLVGYARRDGTNAAKAVMQYLQTKQPSNASPEAVAAKVKALQKPIVYKEDIKRLEA
                     AEAEEAKKRELEWFKYGSNEEMLQAMGLIETA"
BASE COUNT         1673 a         1612 c         1726 g         1724 t
ORIGIN      
        1 tggggaatta ccttggcgca attgagaatt atgtaggctt acaaactgac tatgattgtg
       61 tgtattgcat cgtggatgtt catgcattga ccactgtgga aacgacacag gagcttcgtc
      121 agaacacctt tgaaatggcg ctcgactggc tcgcagcagg gattcgaccg gaagattcga
      181 tcttgtttgt gcaatcacac gtgcctgaag tgatggaatt acacacctat ctttccatgg
      241 tcactccgct tggtaagttg accgatttgc caaccttcaa agagaaagtt cgccaacagc
      301 cagaaaatgt caactatggt ttgctgggtt acccggtttt gatgacggcg gacattgttt
      361 tgtataagac ggatattgtc ccggtcggca ttgaccaggc tccacatatc gaatttgcgc
      421 gcgagaccgt ccgctcgttc aattatcgtt ttggcacaaa agccttgatt gaaccacagg
      481 tcaagcacac tgaagtgaaa aaagtggtcg gtatcgacgg caaggataag atgagcaaga
      541 gcctcaataa tcatattgag ttggcgctca ctgccgaaga gaccaccaaa cgtgtgcgcg
      601 agatggtcac agacccggct cgtcagcgca agacagaccc cggcaaccct gatgtatgca
      661 acgtgttcac catgcacaag atcttctcac cgcaagaaga agtagacatg atcaataccg
      721 aatgccgtaa agctggcatt ggctgtgtgg attgcaaact gcgcttcgct tctaacctca
      781 acaaacatct tgaacccttc cgcgccaaac gcgccgactt tgcttctaaa cctgccgaag
      841 tgcaggaaat cctcaacgat ggcggcaaac gcgcccgcgc cattgctcaa gaaaccatga
      901 gcgaagtccg cgaggcgatg aagttacctt aaacgcaggg gcggggtttc cccgccctat
      961 ttatttctta tgcgcgtcct tatccaacga gtttctcaag ccagcgtcac cgtagaacag
     1021 cagaccattt ccagcatcgg aaaaggtctg ctgattttat tgggcatcgg tcacggcgat
     1081 ggggaagagc aagttcaatt ccttgctgag aagaccgcca acttgcgcat ctttgaagat
     1141 gaacaaggta agaccaacct ctccgttttg gatgtaaaag gtgaagcgat tgttgtttca
     1201 cagttcacgc tctatgccga cagccgtaag ggacgccgcc cctcttttat cgatgctgct
     1261 ttgcccgatg ttgccgaacc gctggtgcat cgctttgtag aactcttacg ttcacatggg
     1321 gttcccactc aaacaggcaa gttcggcgcc catatgcaag tcgaaattca taatgatgga
     1381 cctgtcacca tctggttgga gaagtaatga aacgcccaaa ttttattcaa aagtttgtcc
     1441 atcgtttttt tatgctgaaa ccggtcacag ctttctttgc cactcgtacc cacatcatgg
     1501 acgggattgc ccttaaactc acaggcggaa agtatacggt ttctgaaatt ttggggtgga
     1561 atattgccca gatcatcacc actggcgcga agacaggcga aaaacacacc atcattttga
     1621 ttgctgtcat caacggtgaa aaaattgctc tcattggctc caactttggg cgcaaaccca
     1681 accccggctg gtactacaac ctcaaaaaac accctgaatg cgaagtccac ttgaacggga
     1741 aagcggggat gtatctggca cgcgagaccg agggagaaga gcgcgagtcc tattggaaac
     1801 aggcggttgc gctgtatgca ggctatgaaa aatacaaaga aagagcctca caccgtcgca
     1861 tcccggtcat gctcttagag cctataaaat aattgccaac tttggaattt aatgataaat
     1921 taaggcttga aggatgcaag aggttttcgt tgcggaagga agaagacaac ggctcaaagc
     1981 aagaccttga gccgtatttt ttttaccacc cgatgaatgc aattgcgtca cccaattttt
     2041 tttcattttg ctcaggaggc aaatatggct gaacgtatca tcggcacggt taagtggttc
     2101 aacggaacca agggttatgg tttcatcgct cgcgatggcg gacccgacgt cttcgtccac
     2161 ttttccgcta ttcgcggcga aggcttcaag aacctgcaag aaggccagaa ggtggaattc
     2221 accatcgaga agggccccaa aggtttacag gccgcggatg tagtggttct ctaattctgc
     2281 aagtaattaa ttgaacctaa tgaaagagct tcgtcgtgag acggagctct tttttgttac
     2341 cttttgaaaa aattagggag gctagggaga aggcgcctct ttcgggaccc ataactgggc
     2401 atctgtttcg acggcaattg cccccgctat aagcatggac tcgacatcca tctccgtcca
     2461 caccccgcca gaaccaatga ttgggattcc actcatcact gccgagcgga ccatatccaa
     2521 tgcccgcgga aacagcgact gaccatacat tctgccggtg atgagattgc cattcttgtc
     2581 atacaacgcg ccgcgcggag aggcaatact gatggcactc gcaccaccat ccactagttg
     2641 acgtcccaag gttaatactt gctcatgggg taaggcaaaa ataagcggaa cttcgcccaa
     2701 gcacatttct aaattcatca agataatatc gtctgataaa agtggcgcaa aaccgagttg
     2761 aaccgccatc acattttcaa cattttccag ttcgcgcacc atgcgttgag tttcttcggg
     2821 gcggtctgcc attaagtgga cgatgatggg caggttggca cgattccatt tggcggaata
     2881 ttttttgagc gtggcatgaa agccagggtt gggcagccca gtgtgcatga ggaatccgcc
     2941 ggggaattcg accaaagccg gatgggtagt cggcaggcgc gggcggagag agagcggatt
     3001 cgtcacaaat gccccgaatg aatccaatga gattccattg cgtgggtcgg gtgtgaatcc
     3061 caatgatccc gccgcattga gaagaggttt gctaaaatac agatcgcgtt tcataggaga
     3121 tgatatgcag cccttacaga atatccgcgt gctcgaccta agccgtgtgt tggctggacc
     3181 ttattgtacg atggttcttg gtgacttggg agcggaggtc attaaggtgg agccaccaga
     3241 aggggatgaa acgcgtggct ggggtccgcc atttgcgggt ggagagagcg cttattattt
     3301 atgtgtaaac cgcaataagc gtggcatggt ggtgaatttg aaaacggaag agggacgaga
     3361 tgttctaaaa aaacttgcca tgcaaagcga tgtattcgtg gagaattttc gtcccggtac
     3421 attgaagaaa tttggcttgg attttgaaac tttgcatgaa ttgaacccga agctgattta
     3481 ttgctcaatc tccggcttcg ggcagacggg tccgttgaaa gatatgcccg ggtatgattt
     3541 tatgatccaa gccatgggcg gaatcatgag cgtcactggc gaaccagagg gtgagcccat
     3601 gaaagtgggc gtggctgtgg cagatttatt cgctgggcaa aatgcgatta tctcgatctt
     3661 ggcggcatta caagcgcgga cgtttaccgg cgaaggtcaa tacattgaca ttgccttgtt
     3721 tgattctgaa ttgggttggc tggcaaatgt tgcgagcaat tttctgattt cggggaagaa
     3781 ccccaagcgt tatggcaatg cacatgccaa tattgtgccg tatcaaagtt ttcaagccag
     3841 cgatggttgg tttgtggtgg cagtggggaa tgacaagcag tttgagtctt tttgcaacgt
     3901 gattgaaacc cctgggcttt cgagcgatga aaggttttct acgaattcgg cgcgtgtgca
     3961 aaatcgcgaa gtattaattc ctttgctcaa acctatcttc atgcagaaaa ccgtgaacga
     4021 atggcttgct ctcattggtg accaattccc atgtggaccc atcaatactt ttgaccaggt
     4081 gttctccatg ccgcatgtga aagaacgcga gatgttgata gagatggagc atcccaccat
     4141 tggggcgttg ccactggtcg gttcgccgct taagatgggt ggtacgccgg tctcataccg
     4201 cctcccgccg ccgctgatgg gtcagcacac ccatgaaatt ttgaaagaag tgcttgagtt
     4261 ttccgatgaa gcggtgcaga agttgatgaa tgacgggtgt atcaaataac tttattggag
     4321 ttttacatgg aagtcattta ttctgtatcc ccgttgaagg cattttctgg aagctttctc
     4381 tttttagcat ttttctttgt gttgggcggg tttggcgcgc tctctgccat ctttagccga
     4441 cgtgaccgag cggggaaacg cattgccttg ggttgtgctt cagcaatcat tctgttggct
     4501 ggggcgggcg gagcagtagc tacgtttatg acttataccg gtgggcaaac aaccgctctt
     4561 gtgcacgtgg ataaaaagcg gatcgtagaa cgtaattgtg ataacgcatc aaatacctgc
     4621 attgattatg tcattgaagc cagtgacggg cagaagtatt acacttttgg tttggcgaaa
     4681 gatacttggg atgtagtcca agatcaggct tgttatgaat tcacttatta cccggcaaaa
     4741 tcattgttcg gtgagtattt gcaacaagat aatccctatg ccgatatgta cgagacaacc
     4801 tcaaatatcg tggaaataaa aacggctcag tgcccctgat gagaacctat ttcttgatgt
     4861 gatacatcgt caacagcgga gtccactgtt tggtttcaat ggtcagcccg cgttgagtca
     4921 gatgcgcatc tagggcgttg agtccgaaat cggtgttggg gtgtccccag cggaagaaag
     4981 cgtcgaagac tttgatatcc acaccgcgcg gttcttcgag aaaaaggctg ccgccgggtt
     5041 tgagtacgcg gataatttcg tcaatcgctt tgcgccaatc agggatgtga tgcagcacac
     5101 cgaagataat gacgacatct ttgcttgcat ctgcaaagac actgaggtct tctgcgcctt
     5161 gaagaatgaa ttgaaattgg ggatattgtt ttttcgcaag cgccacttgt tcttccatca
     5221 catccaaccc gatatagctt ttggggttga gagtagaaag cagataggca ccatagccag
     5281 agccacagcc aatttcaagc acatctttat tttgcaggtt gagtcccatg cgtttgaaga
     5341 ggggcatttc aaaataacgt tgacccagct tgcggggcag ggactgcata gcgttgaatt
     5401 caggggcaga tagcttcata aaaaatctcc gagttcatga agaactcgga gattgatttc
     5461 ttacgcggtt tcgatcagac ccatggcttg caacatttct tcgttgctgc cgtacttgaa
     5521 ccattcaagt tcacgcttct tggcttcttc ggcttcagcc gcttcgagcc gtttgatatc
     5581 ttctttgtac acgatcggct tttgtagcgc tttgaccttg gctgccaccg cttccgggct
     5641 ggcattgctg ggctgtttgg tctgtagata ttgcatcacg gctttggcgg cattggtgcc
     5701 atcgcggcga gcatacccaa ccaaaccttc gcttgcctta cgagaccatc cgcccacgaa
     5761 gatgccttcg gttgtggatt cgtaggaagt gccttcgatg gggaacttcg gctctttatt
     5821 tttggagaac tcgccccatt cggtcggcag accaaaattt gcatctacct ggtcaccaat
     5881 ggcgaagatg actgtttcaa catcgagcgt atgttttttg ccggtgccct tggcgctggt
     5941 ttttccatcg cgttcggtca gaatgttctc ttccacttcg agttgggtga gtaaaccatt
     6001 ctcgccaaac atgccagaag gggaggagag gaagtcgaac ttgaacttcg cttctttcac
     6061 cttcgggtct gctttggcaa gcgcatccaa aaccttttga cgcccaatct gcgggtcctg
     6121 accaatcgca tcgagagccg ggcgtacacg agccaattcc ttctcgaact cgtccatgtc
     6181 gagataagca acgaggtgct tcatttcttc cttcgtgaaa ttcacctcag ccggaccgcg
     6241 acgaactacg gcggtcactt cttctacacc ttggtggttg atgagataac gggcaatatc
     6301 caccatcacg ttaccagcgc cgataatggc ggcacgcttt ccatagcgga acttgcgctg
     6361 gctgaacggt ggcaacttgt tatagccata aaccacgtct ttggcgtggt acacgccttc
     6421 aagctcttcg ccgggcaagc ccaaagactt ggtgccctgc gcacccgcag caaccaacac
     6481 cgcgtcaaaa ccgagcgcgc gaatttcatc caatgtgaaa tcaccctttg tgccgatggc
     6541 tacattgcca taatagtcaa tgttcggcat gtcgagcgcc tgacggaact gcttgcgcag
     6601 cccttctttc attgtgtgtt tttcaaggta aatgccgtac tcagccagac caccagcttt
     6661 gatatcacga ttgaaaagag caacgcgcac tccgctattt gctaattcac gtgccccgaa
     6721 aagtcctgcg ggtcc
//
LOCUS       DAOQNJ010000072         6720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6267_length_6720_cov_8.314929, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000072 DAOQNJ010000000
VERSION     DAOQNJ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6267_length_6720_cov_8.314929"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PLG52_03455"
     CDS             complement(<1..1139)
                     /locus_tag="PLG52_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40530.1"
                     /translation="MDTLNEFLSAHHYPLELSNQTLSGSGADFLLARARESRFMLIGE
                     EHGVGSNIDFAAALFRLINRLGYKNYVTEIGPVSARQVNHLLRESDSQAAFEAFYQKY
                     PFSVPFAWMQEEVELLKAVAQHAHEKDNEPVIGIDQEFIFSPQFHFERLHEMCADPAL
                     KTVLEGWLKAEQQAYVDMQNGVSPDKLSVFMNLPLPTGWQELRSWFQAHHADEALTIL
                     DAVQASHVIYRHYVNEEYYDNNNVRALLMRDYFYKAFQRVDSFNPEARFVIKLGANHV
                     SRGFTSMGIQDIGNFISELAPMLGTQSFHVFVLPIRGTLNAWLPFLPESFKAYNVDIN
                     EYGAAVNHVVNHAPVKTGWNLYDLRPLRHRQRHWSKEVPEFQDLF"
     gene            1244..2614
                     /locus_tag="PLG52_03460"
     CDS             1244..2614
                     /locus_tag="PLG52_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40531.1"
                     /translation="MLIIVSDLHLGDGSTADSISPSAFKLFARRLSETARFASIRKDG
                     KYRPVQSMDVILMGDIVDPLHSTRWLDTDPGASNYLRPWSDHTTAAYAQKLHEVTRAI
                     LDENEESLQVMRRLAAGEEIRLAPASSKGEPDYESRVRLPLKVRFHYMVGNHDWYFHL
                     KGPAFDAIRNEMIQRMGLSNPLNTPFPFDAEEAPELKELLERHKVYARHGDIYDRFNY
                     NREAGRDYGTIGDVFTMDVCNRYPVEVSKRYRDRLPAGVVNSLRKIANIRPLLASPLW
                     ISGQIRQFAGNRKLENDLKKVWDEISDEFLQLDFVRQADKAFRFDMVDAMELIVKLSG
                     KASFNTINDVVLWVRKKMWGGKRSFSNYATEEPAFKKGTAQYIVYGHTHSYEIVPLAL
                     NLSMPVPESQIYFNSGTWHSYYDLAMQNPKDQLFVPYQALTYLTFYAAEEHDGRQFEV
                     WSGSYA"
     gene            complement(2648..3466)
                     /locus_tag="PLG52_03465"
     CDS             complement(2648..3466)
                     /locus_tag="PLG52_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40532.1"
                     /translation="MKRKILAGVLVGLSSIILLASLVGMVLAWVYNEPLTLEATTRLD
                     EVETQLTQIQTDLRKAKDEVERALRIIQSAEDALSELTQQAGDAKELLEQVNKTLDDD
                     LIPGLEDTRTKLTDVRSILEDLRNALKQLNSLPFVNFEVPGDDVIANIINGVDSLDTE
                     IADVQGLAKRASTFISDTSYLIGGDFQTTKQNLQDLLVVLKGYDEKLTGWLEQVRMLK
                     ESAPKWIDNASLSLTVVLLWFAFSQLGLILHGLSIWYGENPWEVLKKLKKSSVA"
     gene            3649..3876
                     /locus_tag="PLG52_03470"
     CDS             3649..3876
                     /locus_tag="PLG52_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40533.1"
                     /translation="MSVQTLENTMEKCETTTSDAAPAETPARSLFTRQTGQALITLGT
                     LFKHVPPFNESIDKSTGDTLISIGKKLKGKK"
     gene            3949..>4918
                     /locus_tag="PLG52_03475"
     CDS             3949..>4918
                     /locus_tag="PLG52_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB40534.1"
                     /translation="MAKKNNSSPKLVWDFGTAYELFISLHVLLEPEHFGIRPSYAASV
                     RARIPATERKLLEELYPLLGVPLKWLNTLPGPKDAISALWAIKQIPPAKRMIELYNVH
                     EVYDADKPEDVEKHKTFREILLRIAAEGKVLSTDVDFFQKLFSQKHGNMKREAVESAL
                     DWWSRPAELGEAFLTAFQSYYQAFFEEEEKRIEPILRAGLERAQALSSTVSFEELFTE
                     LSQGLQVGDEFNAPNYIIVPAFWITPLIFFERFDRNTMFLTFGARPADMSVIPGETVP
                     DALVRSLKALADPTRLKIMHYLTHESLTPSEIARKLQLRPPTVTHHL"
     assembly_gap    4919..5018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5019..5216
                     /locus_tag="PLG52_03480"
     CDS             <5019..5216
                     /locus_tag="PLG52_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNB40535.1"
                     /translation="SEIARKLQLRPPTVTHHLKELRLAGLVELSLMHEENRYTARKQA
                     LDAVYEHLNAFLQGENLQELA"
     gene            5213..6493
                     /locus_tag="PLG52_03485"
     CDS             5213..6493
                     /locus_tag="PLG52_03485"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40536.1"
                     /translation="MNTTTSFKPFRILGEYSSHIRAFKPNARLYLLNVIITGAVMGVF
                     RLIFNFYALSLGFDESVLGNLITTSSFVALIAALPMGYLADIIGRKGSLILSSILLAF
                     SVIAMALWKTETSLYAMNVVSGLAQSLAGVTMSPFLMENSDEKERTYLFSFGQGLQMT
                     MASIGNWVGGYLPTWISQTQHVSETSSIAYGNSILIAGIGALIAVLPLMFIKSPNITR
                     SQRAVFAPFQYAAKNPGLLTKLVLPMLLTSLGAGLIMPFMNVFFRVVHHQPDPVIGTL
                     FAWGSLAMGIGLLIAPPLADRTGKIQLVVITQALSIPFLILLGFSPIFWVGAASYYIR
                     LALMNMSSPVYQTFVMEHVEPSGRATVASLTSMAWNFGWAFSPTISGWLQVKYGFGPA
                     FIGTITLYTISVLMYWAFFWRRSIDPVPASAAAD"
BASE COUNT         1689 a         1866 c         1428 g         1637 t
ORIGIN      
        1 aagaacaagt cttggaattc aggcacttcc ttcgaccaat gtctctgccg atggcgcagc
       61 ggacgtagat catataaatt ccagcctgtt tttacaggcg catgatttac tacatggttt
      121 acagccgcgc cgtactcgtt gatgtccacg ttatacgcct tgaacgactc tggtaagaag
      181 ggcagccaag cattcaacgt gccgcgtatc ggaagcacaa acacatgaaa cgactgtgtc
      241 cccaacatgg gcgcaagttc agagataaaa ttcccaatgt cttgaatgcc catagaggtg
      301 aacccgcgcg aaacatgatt cgcgccgagt ttgatcacaa aacgcgcctc tgggttgaac
      361 gagtccacgc gttgaaacgc cttataaaaa taatcccgca ttaaaagcgc gcgcacattg
      421 ttattatcat agtactcttc gttcacataa tgtcgataga taacatggct ggcttgcacc
      481 gcatccagga tggttaacgc ctcgtccgca tgatgggctt gaaaccaact ccgtagttct
      541 tgccagcctg taggcaaggg caagttcata aacaccgaaa gcttatccgg cgaaacccca
      601 ttctgcatat caacataggc ttgttgctct gccttcaacc aaccctcaag cacggttttc
      661 aaggctgggt ctgcgcacat ctcatgcaaa cgctcaaaat gaaattgcgg gctgaaaata
      721 aactcctgat cgatcccaat cacaggctcg ttatcttttt catgcgcatg ctgtgcgaca
      781 gctttcaata actccacttc ttcttgcatc cacgcgaacg gcaccgaaaa cggatacttc
      841 tgataaaacg cctcaaatgc agcttgtgaa tctgattcgc gcaacaagtg attcacctgt
      901 ctcgcactca ccggtccgat ctcggtgaca taattcttat atcccaatcg gttaatcaac
      961 cgaaacaacg ctgccgcaaa atcgatattg ctccccaccc catgctcttc accaattaac
     1021 ataaagcgag actctcgtgc acgcgccagt aaaaagtctg cgccagagcc gctcaatgtt
     1081 tgattggaaa gttccaaggg gtaatgatgt gcagaaagaa attcattcaa ggtatccatt
     1141 aacgtctcca ctaaaaggtt tcagacttgc gaatcacttt cttttgaaaa attataatcg
     1201 gacaaatccg aatttcatcc aatcttcgcg ctataatctg cttatgctta tcatcgtcag
     1261 cgacttacat cttggggatg gctccaccgc agactccatc tcccccagtg cattcaaact
     1321 cttcgcgcgg cggctttctg aaaccgcccg tttcgcttcc attcgcaaag atgggaagta
     1381 tcgccccgtt caaagcatgg atgtgattct catgggcgac atcgtcgacc cgcttcattc
     1441 cacccgctgg ctggatactg accccggagc ctccaactat ctgagaccgt ggagcgacca
     1501 tactaccgcc gcgtatgctc agaaattgca tgaggtcacc cgcgccatct tagatgaaaa
     1561 cgaagagtcg ctgcaagtca tgcgtcggct cgcagccggc gaagagattc ggcttgcccc
     1621 agccagcagc aaaggcgaac ccgattacga aagccgcgta aggcttccgc tcaaagtccg
     1681 ctttcattat atggtgggca atcacgattg gtatttccat ctcaaaggtc ccgccttcga
     1741 tgccattcgc aatgaaatga ttcaacgcat ggggctgagc aacccgctca atacaccctt
     1801 cccgttcgat gcagaagaag cacctgaact gaaagaactt ctcgaacgac acaaagttta
     1861 tgcccgccac ggcgatattt acgaccgctt caactataac cgtgaagccg gtcgcgatta
     1921 tggcacgatt ggcgatgtat tcaccatgga cgtttgcaat cgctaccccg tagaagtttc
     1981 caaacgctat cgagaccgtc tccctgccgg ggtggtcaac agcctgcgca agatcgccaa
     2041 catccgccca ttgctcgcct ctccactttg gatcagcgga caaatccgtc agttcgccgg
     2101 gaatcgcaaa ctggaaaatg acctgaaaaa ggtctgggat gagatttcag atgaattcct
     2161 gcaattggat tttgtgcgtc aagccgataa agccttccgc ttcgatatgg tcgatgccat
     2221 ggagttgatc gtcaagttat cgggcaaggc ttcttttaac accatcaacg acgtggtcct
     2281 ttgggttcgc aaaaagatgt gggggggcaa acggtctttt tccaactatg caacggaaga
     2341 acctgctttc aaaaaaggga ctgctcaata cattgtctac ggacataccc atagttatga
     2401 gatcgtgcca ttggcattga acctgtctat gccggtgcca gagagccaga tctacttcaa
     2461 ctcaggaacc tggcattcgt attatgacct tgccatgcaa aacccaaaag accagctatt
     2521 tgtgccttac caagccctca cctatctaac tttttacgcc gccgaagaac atgatggtcg
     2581 gcagtttgag gtttggtctg ggtcgtatgc ctaaaaaaga aacggtctca aatgagaccg
     2641 tttcttttta tgctacagaa ctcttcttta atttctttaa aacctcccac gggttttcgc
     2701 cataccaaat gctcagcccg tgcagaatca gcccgagctg agagaaggca aaccagagca
     2761 gaacaactgt cagggagaga gaggcgttat cgatccactt aggcgcggac tctttcagca
     2821 tccgcacttg ttcaagccag ccagtcaact tctcgtcata tccttttagc accaccaata
     2881 aatcttgtag attctgcttg gtggtttgaa aatcaccgcc gatcaaatag gatgtgtcac
     2941 taataaaggt agaagcgcgt ttggcaagcc cttgcacgtc tgcgatctcg gtatccaatg
     3001 aatcgactcc gttgatgatg ttggcaatca catcgtcacc cggcacttca aagttgacga
     3061 agggcagcga attcaactgt ttcaaggcat ttcgcaagtc ttccaaaatc gagcggacgt
     3121 ctgtcagctt tgtcctcgta tcttccaagc cgggaatcag gtcgtcatcc aaagtcttat
     3181 tgacctgttc caacagttcc ttcgcgtcgc cagcctgttg tgtcaactcc gaaagcgcat
     3241 cctccgcaga ctgtatgata cgcaatgcac gctctacttc atctttcgct ttgcgcaagt
     3301 cggtttggat ctgagttagc tgggtttcca cctcgtccaa ccgcgttgtg gcttcgagag
     3361 tcagcggctc gttgtaaacc cacgccaaaa ccatccccac caaactcgcc aacaaaatga
     3421 tggaactgag tccaaccagc acccccgcca agatttttcg cttcatggtt ccgtcctttc
     3481 atatagtaga accctgccgc tgccaaatca gaatgaccca aaactcctat ttgactagaa
     3541 atacccccta cctacttgac aaagcgtcta agttagacta ttatctaatt agatgtacgt
     3601 ctaatacagt catttgccta accgtcccca tcagagagag aggacaccat gtcggtacaa
     3661 accttagaga acacaatgga aaaatgcgaa accaccacat cagacgccgc tccggctgaa
     3721 accccagccc gaagcctctt cacccgccaa accgggcagg cgctcatcac gcttggcacc
     3781 ctgttcaaac atgttccccc attcaatgaa agcatcgaca aaagcaccgg ggatactctc
     3841 atctccatcg gtaaaaaact aaaaggaaaa aaataaagcc cacacttcag gtgacagtcc
     3901 aatcgatggg ctgtcacctg aactttgaat acgggcacac tactatatat ggctaaaaag
     3961 aacaattcct ctccaaaact cgtttgggat tttggcaccg cctacgagtt attcatcagc
     4021 ttacatgttc tgcttgaacc cgaacatttc ggcattcgcc catcgtatgc cgccagtgtt
     4081 cgagcccgta ttccggcaac agaacgaaaa ctgttagagg agttgtatcc gcttttgggt
     4141 gttccgctta aatggctaaa taccctgccc ggacccaaag atgccatcag cgcactttgg
     4201 gcgattaaac aaattccgcc cgccaaacgg atgatagaac tgtataacgt tcacgaagtg
     4261 tacgatgccg ataaaccaga agatgttgaa aaacacaaaa cctttcgaga gattctgttg
     4321 cgcatcgcgg ctgaaggcaa agtcctttcc accgatgtag attttttcca aaagctcttc
     4381 tcgcaaaaac acggcaacat gaaacgcgaa gccgttgaat ccgctttaga ttggtggagc
     4441 agacccgccg aactcggtga agcgttcctc actgcttttc aatcctacta tcaagcattt
     4501 tttgaggagg aagaaaagcg catcgaaccc atcctgcgtg ctgggttaga aagagcccaa
     4561 gctttatcct ccactgtcag ctttgaagaa ctattcaccg agctttctca aggtcttcaa
     4621 gtaggcgatg aattcaatgc tcccaactac atcatcgttc ctgcattttg gatcaccccg
     4681 ctaatcttct tcgaacgctt tgaccgcaac accatgttcc tcaccttcgg cgcccgcccc
     4741 gccgatatgt ctgtcatccc cggcgaaacc gtacccgatg cgttggtccg ttcgctcaaa
     4801 gcccttgcag accccacgcg cttgaagatc atgcattacc tcacacacga gagcctgacc
     4861 ccttctgaga tcgcccgcaa gttgcagctc cgcccaccca cagtgacgca tcacctcann
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc tgagatcgcc cgcaagttgc
     5041 agctccgccc acccacagtg acgcatcacc tcaaagagct tcgtctcgcc ggtttggtgg
     5101 aactttctct catgcacgaa gagaaccgtt acaccgcccg taagcaagcg ctcgatgcag
     5161 tgtatgaaca tctaaatgcc ttcctgcaag gcgaaaatct acaagaactc gcatgaacac
     5221 cacaacctct ttcaaaccct tccgtatctt gggagaatat agctcccaca tccgcgcatt
     5281 caaaccgaat gcgcggcttt atcttctaaa cgtcatcatc accggtgcag tgatgggcgt
     5341 tttccgcctc atcttcaact tctacgccct gagcctcggc ttcgatgaat cggtgttggg
     5401 aaatctcatc accaccagca gtttcgtcgc tctgatcgcc gctctgccca tgggctatct
     5461 ggcagacatc atcgggcgca aaggttcgtt gattctttcc agtatcttgc tggcattttc
     5521 tgtcattgcc atggcgctat ggaaaaccga aacatcgctc tatgccatga acgtcgtttc
     5581 tgggttggca caaagtttag caggcgtgac catgtctccc ttcctcatgg aaaatagcga
     5641 cgagaaagag cgcacctacc tcttcagctt tggtcagggc ttgcaaatga ccatggcatc
     5701 cattggcaat tgggttggcg gctacctccc cacttggatc agccaaaccc aacacgtctc
     5761 tgaaaccagc agcatcgcgt atggaaattc cattctcatc gccggaatcg gcgcattgat
     5821 tgctgtgttg ccattgatgt tcattaagtc accgaacatc acccgaagtc aacgcgctgt
     5881 ttttgcacct tttcaatacg cggcgaagaa ccccgggctt cttacaaaac ttgtcctgcc
     5941 aatgttgctc acctcgttag gtgcgggtct catcatgcca ttcatgaatg tcttcttccg
     6001 cgtggttcat caccaacccg acccagtcat tggtacactt ttcgcttggg gttcactggc
     6061 aatgggcatt ggtctgctta tcgcgccacc tctcgcagac cgaacaggca aaatccaact
     6121 cgtggtaatt acacaagcgc tttccattcc atttctcatc ttgcttggtt tctcccccat
     6181 cttttgggtt ggcgctgcca gctattacat ccgcctcgcg ctcatgaata tgagcagccc
     6241 cgtttaccaa actttcgtaa tggaacacgt ggagccatca gggcgtgcca cggttgcaag
     6301 cctcaccagc atggcatgga acttcggctg ggcgttcagc cctaccatca gtggttggtt
     6361 gcaagtgaag tatgggtttg gtccggcatt cattggaacc atcacactct atacgatctc
     6421 ggtccttatg tattgggcat tcttctggcg tcgaagcatc gacccggtcc cagcttcggc
     6481 agccgcagat tagaaaagtc tcaccgattt tggaaaaaca gagcattgaa attgctctgt
     6541 ttttttataa aatatcttaa tggacaaaga tatcttgatt attggcggcg gtccgagcgg
     6601 actctcgact gcccttcatc tcgcaaaata tgccccgcat ctgactcagc gtattttgat
     6661 cctcgaaaag gaacattatc cacgcctgaa gctttgtgct ggcggcttgg tcatcgatgc
//
LOCUS       DAOQNJ010000073         6676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6336_length_6676_cov_8.684942, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000073 DAOQNJ010000000
VERSION     DAOQNJ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6336_length_6676_cov_8.684942"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PLG52_03490"
     CDS             <1..1068
                     /locus_tag="PLG52_03490"
                     /inference="COORDINATES: protein motif:HMM:NF036809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNB40537.1"
                     /translation="WPSSPSSDVPFEDPNGQIQGDAHYWEVWHRGKPFTAYRDQYPRF
                     MSEFGFQALPPLETIRTFAEESEWNMTSYIMERHQKNDSGNSLIVRQMLDTFRLPKDF
                     DALVYLSLVLQAEGIRYGVEHWRRHTNRVSGTLYWQLNDCWPVASWASLDYFGRWKAL
                     HYAARRFYAPLMLSIEDAPPKQSIFISSDLMKDWSGKVRWSLVDLDGKVLTSGEKAAS
                     VSVNGVTAVESLDFSQYLNDDLRRELAFIAELWEGDTCLARQSAFFAPTKHLKLKAPQ
                     ITSTLSAKDGVLSIEISSKSFARLVEVSLSGADVVFSDNYFDLPANTKVTVTAPLPAG
                     WDLKKAKAAFKVRSVFDTYSN"
     gene            1082..3400
                     /locus_tag="PLG52_03495"
     CDS             1082..3400
                     /locus_tag="PLG52_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNB40538.1"
                     /translation="MATDIKSIISQMTLEEKAALCTGASNWSTTPVERLGLPEMIVSD
                     GPHGVRRVADVHSMGQKSLPATCFPTASCTASTWDVDLIKQMGIALAEECIALNVDVL
                     LGPGVNMKRSPLNGRNFEYFSEDPFLAGELAANFINGVQSKGVGTSLKHYAANNQEFQ
                     RFSISAEIDERTLREIYLPAFEKAVKQAQPWTVMCSYNKVNGTFASEHHQLLTKILKD
                     EWGFEGLVVSDWGAVRNRVAALKAGLDWEMPGPQDLRVKAVVEAVRSGELNEATLDES
                     VRRILRIVFKSKETPKGNGSFDVDAHHELARKIATEGVVLLKNDGLLPLKDHQHIAVI
                     GRSAESAHFQGGGSSHINPTKVAMPYKELQSRAENAELTYAEGYPTDNSFQQGMIDDA
                     VGLAEAADVAILYIALPTFKESEGYDRKDLDLTVQQIALIKAVSKVQPNTVVVLNNGA
                     PVAMSEWIDGVPAVLEGWMMGQAGGAALADILFGRVNPSGKLAETFPLKLTDTPSHIN
                     WPGGAGKVHYGEGIFIGYRYYDTKEIPVLFPFGHGLSYTTFEYSNAKVSSKNFKDVDG
                     LTVTVDVKNTGKLAGKETVQVYVHDQKSDLMRPVKELKGFAKVDLQPGETKTVSIHLD
                     FRAFAYYHPEHKQWITEDGDFDILIGSSSADIRSHLTTTLQSTLDLPCVLDIESTMRE
                     WMADRRGRQVLGSLYEQIESQVRRLFAGEDRYGNTKAEEQVAEQTVMGMDIMDMMVDM
                     PVVSVLMFQQSSLTMPAEEMVAGLLRQVHSMK"
     gene            3572..4846
                     /locus_tag="PLG52_03500"
     CDS             3572..4846
                     /locus_tag="PLG52_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40539.1"
                     /translation="MLNNHPLIQSLRELKGNPRITVWTEIMFGIPYNLFMPFFSVYML
                     ALGVTDQQIGSIASMGLVAQVFTALISGAIVDKFGRRLTLFIGDLLCWSVPCFIWAFA
                     QDVRFFVIAALMNSLWRISHTAWTCLMVEDAETRHLVHIWTWIMIFAFSSAFFAPLGG
                     WFVERYGLISAMRGLLIFGFVMLTAKGILLYIYSHETGRGIQRREETRHRSIISLLAE
                     YRSVFGQILHSRAILSALTLLVIMNIYSTITNNFWGVLFSSKLGFSDSQISTYVALRS
                     IVMTLGFFLIGPRLTNLRRFRLPLGAGFGVFLLSQVLLVLMPPKAVLFLVLSVVLEGA
                     GAALVNPMTESLLAVSMETDERARLTAMVSMAMIILISPFGWIAGQLSALDRSYPFLL
                     NIGLFAIGLVIVWIIGKPGFIPFVKQTEQQEI"
     gene            4863..5855
                     /gene="glk"
                     /locus_tag="PLG52_03505"
     CDS             4863..5855
                     /gene="glk"
                     /locus_tag="PLG52_03505"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNB40540.1"
                     /translation="MNVLVGDIGGTKTALAIVSSETGPRELSNEKHFLSSQYDSVDEI
                     IHAYLEDAAVQIDAICLVVAGPVIDGKAKITNLPWVMEEVALAEKFKVTYAKLLNDLE
                     GMAYAVPILNEEDVFTLSAGKPVSGGSIAVIAPGTGLGEAYLTKDSANYCAHASEGGH
                     ASFSPINALQIELLEFLQHKKGLEHVSMERVCSGSLGIPNLYTFLKESGKYSEPDWLS
                     QKFADGVDMAPIIVGAAQDPENPSELCIATLDLFCSILAVEAGNLALKILSTGGIYFG
                     GGIPPRILERLQKPEFLATLRHKGRFEGLLTQMPVKVILNTKAPLMGAGEYGLR"
     gene            6069..>6676
                     /locus_tag="PLG52_03510"
     CDS             6069..>6676
                     /locus_tag="PLG52_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNB40541.1"
                     /translation="MAGKTKIRAAAVQFGVVADVAANLQTCLRMIDEAAKVKPDVMVL
                     PEFVNHIAWYRDAAHCYGVAVSLDDDFIHAIGRKAKEHGCYIKINVTLKRDDGKVSGT
                     NILFDPAGNVVGLTDKQILMGNENNFLEKSTTIAPILETELGHIGMYACMDGVIPEVA
                     RALAVRRPQMLLNSLNSFAHDEASLHIPVRAAENKVFVVAAN"
BASE COUNT         1645 a         1510 c         1678 g         1843 t
ORIGIN      
        1 tggcccagtt cgccttcttc agatgttcca tttgaagacc cgaacggaca aattcaaggt
       61 gatgcacatt attgggaagt atggcacagg ggcaaaccat ttacggcgta tcgcgatcaa
      121 tatccgcgct tcatgagtga gtttggtttt caggcgcttc caccgcttga gactattcgt
      181 acctttgctg aagaatccga atggaacatg acctcttaca tcatggagcg tcaccagaag
      241 aatgacagcg gcaactcgtt gatcgttcgg cagatgcttg atacgttcag gcttcccaaa
      301 gactttgatg cgttggtgta tctcagcctt gtgttgcaag ccgaagggat acgctatggt
      361 gtagagcatt ggcgcagaca taccaaccgc gtctctggca ccttgtattg gcagttgaac
      421 gattgctggc ccgtcgcttc gtgggcaagc cttgactatt ttgggcgttg gaaggctctg
      481 cactacgctg cccgtcgctt ctatgcgcca ttgatgcttt ccattgaaga tgcaccaccc
      541 aagcaatcaa tcttcatcag cagtgatttg atgaaagatt ggagcggtaa ggtccgctgg
      601 tctttggtgg acttggacgg aaaggtgctg acctctggtg aaaaagctgc ctcggtttcg
      661 gtaaacggag tcacagccgt tgagtcatta gacttctctc aatacctgaa cgatgacttg
      721 cgccgtgagc ttgccttcat tgctgagcta tgggaaggtg atacctgcct tgctcgtcaa
      781 tccgctttct ttgcaccaac caagcacctc aaattaaaag ctccacagat cacatccact
      841 ttgagtgcga aagatggagt gctttccatt gaaatttcat ctaagtcgtt cgcgcgcctt
      901 gtggaagtta gcctaagcgg tgcagatgtg gtcttcagcg ataactattt cgatctacct
      961 gcaaatacca aggtgacggt aacagcacct ttgcccgcag gttgggatct caaaaaagcc
     1021 aaggcggcat tcaaagtccg ctctgtattt gatacgtatt ccaattaaaa aggaaaagac
     1081 tatggctaca gatattaaat ccatcatttc tcaaatgacc cttgaagaga aggcggctct
     1141 ttgcactgga gccagcaact ggtctaccac acctgtcgag cgtttggggc tgcccgaaat
     1201 gattgtctcc gatggtccgc atggagtgcg ccgtgttgcg gatgtccact cgatgggaca
     1261 aaagagtttg cctgctacct gtttcccaac cgcatcctgc accgcctcca cctgggatgt
     1321 ggacctgatc aagcaaatgg gcattgccct cgctgaagaa tgcatcgctc tcaatgtgga
     1381 tgtcttgctt ggtcccggtg taaatatgaa gcggtctccg ctcaatgggc gtaactttga
     1441 atatttctcc gaagacccct tccttgcagg cgaattggct gctaacttca tcaatggcgt
     1501 gcaatctaaa ggcgtaggaa cttcgctcaa acattacgcc gccaataatc aagaattcca
     1561 gcgcttcagc atcagtgccg agattgacga acgcaccctg cgtgagatct atctacccgc
     1621 gtttgagaaa gctgtcaaac aagctcaacc gtggacggtg atgtgctcat ataacaaggt
     1681 caatggcaca tttgcatctg aacatcatca attgcttacc aaaatcctca aagatgaatg
     1741 gggctttgaa ggtttggtcg tgtccgactg gggcgcggtg cgcaatcggg tcgcggctct
     1801 caaagcgggt ttggattggg aaatgccggg tccgcaggac ttgcgtgtca aagctgtggt
     1861 cgaggcggtt cgctctggtg agttgaacga agccacattg gacgaatccg ttcgccgtat
     1921 tttgcgcatc gtttttaaat ccaaagaaac ccctaaaggg aacggctcct ttgatgtgga
     1981 tgcccaccat gaactggcac gaaagatcgc cacagaaggt gtggtgctgt tgaagaacga
     2041 tggtctatta ccgttgaaag atcatcaaca tatcgcagtc attgggcgct cagcagagtc
     2101 cgcgcatttt caaggcggtg gcagctcaca catcaacccg accaaagtcg ccatgcctta
     2161 taaggaactt caatctcgcg ccgaaaacgc cgagttgacc tacgccgaag gctaccccac
     2221 cgataactcc ttccagcaag gtatgatcga tgacgcagtg ggtctcgcag aggctgctga
     2281 tgtggcaata ctttacatcg ctttgcccac tttcaaagag tccgaaggct atgaccgaaa
     2341 agacctagac ctcacggttc aacagattgc attgatcaaa gctgtttcca aagttcagcc
     2401 gaatacggtt gtggtgttga ataatggtgc gcctgttgcc atgagcgaat ggattgatgg
     2461 tgttccggct gtgctcgaag gttggatgat ggggcaggca ggcggcgcgg ctcttgcaga
     2521 cattctcttt gggcgtgtga accccagcgg caaactcgca gaaaccttcc cgctcaaact
     2581 taccgacact ccatcccaca tcaattggcc cggcggcgct ggcaaggttc attatggcga
     2641 aggcattttc attggttatc gttattacga caccaaagaa atacctgtac tcttcccatt
     2701 tgggcacggt ttgagctaca ccacatttga atatagcaac gcgaaagtat cttccaaaaa
     2761 tttcaaagat gtggatgggc ttacggtgac tgtcgatgtt aagaacacgg gcaaactcgc
     2821 aggtaaagaa acggttcagg tgtacgtcca cgaccaaaag tctgatctga tgcgcccggt
     2881 caaagaattg aagggctttg ccaaagtgga cctccagccg ggcgagacca agaccgtttc
     2941 catccatttg gatttccgtg ccttcgctta ctatcacccc gaacataaac agtggattac
     3001 cgaagacggc gactttgaca tccttattgg ctcttcttca gctgatattc gtagccacct
     3061 caccaccacg cttcaatcga ccttagatct gccctgcgtt ctggatattg aatctacaat
     3121 gcgagagtgg atggcagatc gacgtggtag gcaggtgctt ggtagcctct acgaacaaat
     3181 cgaaagccaa gtccgcaggt tgtttgccgg tgaagaccgt tatgggaata caaaggcaga
     3241 agaacaagtt gctgaacaaa ctgtgatggg catggatatc atggacatga tggttgatat
     3301 gcctgtagtg agtgtcttga tgttccagca aagctcactg actatgcccg ccgaagagat
     3361 ggtcgctggg cttttacgac aggttcatag tatgaaatag ctcttttggg atgaaggtta
     3421 tcaaagcctt catccctatt ttttttctaa aggcttgatg taacttgacg tggcgctgtt
     3481 cattgcgaat agcgccatta ttgacgctat tttcaagcct gttcaactgg tttgacctat
     3541 agaaagcttt tggtaatatt gcaggactac aatgttaaat aatcatcctc ttattcaatc
     3601 ccttcgtgaa ctgaagggaa acccacgtat tacggtctgg accgagatca tgttcggtat
     3661 tccatacaac ctttttatgc cattcttttc tgtatacatg ctggctttgg gtgtcaccga
     3721 tcaacaaatc ggtagcattg ccagtatggg tttggtagct caggttttca ccgcgctcat
     3781 aagtggcgcc attgtcgata aatttgggcg acgcctgacg ctttttattg gcgatctatt
     3841 atgctggagc gtgccatgct ttatctgggc atttgcacaa gatgtgagat tctttgtcat
     3901 tgcggctttg atgaatagcc tttggcgcat ctcacacacc gcatggacat gcttaatggt
     3961 cgaagatgct gaaacccgtc acctcgttca tatttggaca tggattatga tctttgcttt
     4021 ttcttcggcg ttttttgcac ctttaggcgg ctggtttgtc gaacgatatg gcttgatctc
     4081 tgccatgcgc ggattgttga tcttcggttt tgttatgtta actgccaaag gtattttgct
     4141 ttacatatat tcgcatgaaa cggggcgtgg catacaacgc agagaagaaa cccgtcaccg
     4201 ttctatcatt agtttgttgg ctgaatatcg cagtgttttc ggacaaatat tacattcacg
     4261 cgccatcctt tctgcgctaa cattgctcgt cattatgaat atctactcaa ccatcacaaa
     4321 taatttttgg ggtgtgttgt tttccagcaa attaggcttt tctgactcgc aaatttccac
     4381 ctatgttgcc ttgcgctcaa ttgtaatgac tttgggcttc tttttgatcg gtccacgcct
     4441 caccaatttg cgccgattcc gtttgcctct gggcgcaggc tttggtgtct ttttattgag
     4501 tcaggtgtta ttggttctta tgccgcctaa agctgtgtta ttcctggtgt tgagcgtggt
     4561 tttggaaggc gctggcgccg cgcttgttaa ccctatgacg gagtcgttgt tggcggtttc
     4621 catggaaaca gatgaacgcg ctaggctgac tgccatggtt tccatggcaa tgattatctt
     4681 gatttcgccc tttggctgga tcgcaggtca actctctgcc cttgaccgtt cctacccatt
     4741 tttattgaat attggcttgt ttgctatagg gcttgtgatt gtttggatta taggaaaacc
     4801 cgggttcatt ccatttgtaa aacagacaga gcagcaagaa atttagattt gaggtaatta
     4861 cgatgaatgt attagtaggt gatatcggcg gtacaaaaac tgctttggct attgtctctt
     4921 ccgagacagg tccacgtgag ctttcaaacg aaaaacattt tctaagctct cagtatgaca
     4981 gtgtggatga gatcatccat gcttatttag aagatgccgc agtacagatc gacgcgattt
     5041 gtttggtggt ggcgggacct gtaattgacg gaaaggcaaa gatcaccaac cttccatggg
     5101 taatggaaga agtagccctc gcggaaaaat tcaaggtgac atatgccaaa ctgttaaatg
     5161 accttgaggg catggcttat gctgttccta tattaaatga ggaggatgtg ttcaccctat
     5221 ctgctggaaa acctgtcagc ggtggctcga ttgcagtgat agccccaggc acagggttag
     5281 gtgaggcgta tctgactaaa gatagtgcaa actattgtgc tcatgcttca gaaggtgggc
     5341 atgcatcctt ttctcccatt aatgctttac aaatcgaatt actagaattc ttacaacata
     5401 aaaagggact tgaacatgtt agtatggagc gggtttgttc cggctcgctt ggaataccaa
     5461 atctgtatac attcctaaaa gaatctggga agtacagcga acctgattgg ctctctcaaa
     5521 aatttgcaga tggcgtagat atggctccca tcattgttgg tgcggcacaa gacccagaaa
     5581 atccgtctga gctttgtatc gccactctcg atttgttttg ttcgatcctt gctgttgagg
     5641 cagggaactt ggcgttgaag atattatcta ctggtggcat ttattttgga ggcggcatac
     5701 cgccacggat tcttgagaga ttgcaaaagc cagaatttct cgccactttg cgccataagg
     5761 ggcgctttga agggttgctc acacaaatgc ctgtcaaagt aatattaaat accaaagcac
     5821 ccctaatggg cgccggagaa tatggtttga ggtgagtgct taagcatata gctctaaatt
     5881 cgattcttgt aaaagccatg ctttgtggga caactcaaag catgttacat caagctacaa
     5941 tgtgacgaaa tagaaggagc aatatttaaa gtgtagtgag ctccttctaa atatttctga
     6001 gttgacaaag atggcttttt taaggtatat tgttataatg ttataacatt tagccttgga
     6061 ggcagttttt ggctggtaaa actaaaattc gagcggcagc ggtacagttt ggcgtggtgg
     6121 cggatgttgc tgcgaacctg caaacctgtt tgcggatgat cgacgaagcg gcaaaggtca
     6181 agcccgatgt gatggtgctg cctgaatttg tgaatcacat cgcctggtat cgtgacgcgg
     6241 ctcattgcta cggggtggct gtttcgctcg acgatgactt tatccacgcg ataggcagaa
     6301 aagccaaaga gcatggctgc tatatcaaga tcaacgtcac gttgaaacga gatgatggca
     6361 aggtttcagg cacgaatata ttgttcgacc cagcggggaa tgtggtcggg ttgaccgata
     6421 agcagatttt gatgggcaat gaaaataact tccttgagaa gtccacaaca attgcgccga
     6481 ttttggaaac agagttgggg catataggca tgtacgcctg catggatggt gtcatccctg
     6541 aggtggcgcg tgcgttggcg gtgcgtcgtc cgcagatgtt gctcaacagc ctgaattcat
     6601 ttgcacatga tgaagcgtcg ttgcatatcc ctgtgcgggc ggcagagaat aaagtattcg
     6661 tcgtcgcggc aaacaa
//
LOCUS       DAOQNJ010000074         6665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6353_length_6665_cov_7.871861, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000074 DAOQNJ010000000
VERSION     DAOQNJ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6353_length_6665_cov_7.871861"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLG52_03515"
     CDS             complement(<1..461)
                     /locus_tag="PLG52_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40542.1"
                     /translation="MKRPNEKNFYIFVSLLLEGVGAGMIIIPSHFGITHKILSIIPLW
                     ILGFSCFGTALAIQIHCVWGLTHKREALIGVIATTLLFIVSFFLIQSRQYISAAAMVI
                     AGIYQTYIILPINKKLKQVDFASLSIAAITFATGIILSVTSAGDDAKLLLAA"
     gene            624..1460
                     /locus_tag="PLG52_03520"
     CDS             624..1460
                     /locus_tag="PLG52_03520"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNB40543.1"
                     /translation="MQKFISLAQIDEIVNVIKKKISIQPVIGMILGSGLNGLADSVQN
                     PVHIPYSDLPNFPTSTVHGHVGRFVIGELEGKPVIVMQGRIHYYEGYTMGEVTLPVRV
                     MHRLGVHSLFVTNAAGGVNENFVPGDVMLITDQLNLMGMSGLNPLMGPNLDEIGPRFP
                     DMSQPYDREYCDLARKVAKQSNLTLREGVYAGLSGPSFESPADLRFLRLAGADAVGMS
                     TVPEVIIARHGGMRVLGLSGVSNKANLDGSTITTHEEVIEAGRVITPKIETIVRGVLR
                     GI"
     gene            1485..2162
                     /locus_tag="PLG52_03525"
     CDS             1485..2162
                     /locus_tag="PLG52_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40544.1"
                     /translation="MVDLFKFLAEYEVLIYIVLAIGALFSFRWLFRSWREWQVAVFSL
                     EREFSARRFGRSALVSAMIVIFFCVEFFMASFIIPGLPADFFVATPTLDLISTPTGTL
                     SPELMTQFANQPQVAQPNAAGCTPGQIELTFPEAGTEIKGIIELTGTVDIPNFGFYKY
                     EIAPSGSDTWATIAAGRTVVKASSLGRWDTTALTPGDYQLRLVVIDNEGNSLTPCVVP
                     VQVIPNQ"
     gene            2179..2718
                     /locus_tag="PLG52_03530"
     CDS             2179..2718
                     /locus_tag="PLG52_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40545.1"
                     /translation="MSELQIRPTVATDLTRLMGIDHSMTSEAVWQLEVRRSTGQVAVT
                     FREVRLPREIQVPYPHNHFALADDWVKRSMMYTAIKDAEPVGYISLLERGTASTVWVL
                     DLAIDAKQRRRGVAAALLASAQAWAESRSHRRLILEVQSKNVPAIRLAQKSGFEFCGY
                     NDQYYLNKDVALYFAKVLK"
     gene            2767..4680
                     /locus_tag="PLG52_03535"
     CDS             2767..4680
                     /locus_tag="PLG52_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40546.1"
                     /translation="MINGWPETLLAGLQTINQILTAGIAITAFSLFLYSLSFNLRDRV
                     ARSFAIILLCVVIVFTAESLQDNSVTIKALDLLMRLQWFGLAFLPAAYLHLSDALLVT
                     AGRPSRGRRRFAVRGMYIISALFLVLLAFGYLLGPIVPDGKPAPYLQRTAWTEVFTIF
                     YVTSMVWAGVNFYRAYTLMLTRSGRRRMIYLMAGATAPALGSYPYLLFGYSLASQHQF
                     LFWTAATVINALVGALVIVMAYAVAFFGVSWPDRVIKSRLFKWIMRGPVVASATLALM
                     TVVRRGGELLGTPYSAFVPFTVVASILLMEHAITFAAPLWERGLFFGRDRNELELLQN
                     IEERLLTQGDLQQFLEAVLAAVRDHLQSPSAFVAALDDETLLPVVVAGNRALLEQESL
                     TEALEHINGDVRHEFQWGNYWVLPLYSRRRPEMDEDELPPLLGLLGVAKNGKRDIMEQ
                     DQRESLWLLADRAALALEDRQLQQRVFRSLADIQPQVEMIQRMRAASRYDTNANLLTA
                     PLPREADLANWVKDALTHYWGGPKFTNNPLTKLQVVQELADKEDGNISNALRTLLRQA
                     VDEVKPRGDRKFTTEWILYNILEMKFIEGRKVRDVAARLAMSEADLYRKQRVAIEAVA
                     KAILEMEVSKLEQ"
     gene            4774..5739
                     /locus_tag="PLG52_03540"
     CDS             4774..5739
                     /locus_tag="PLG52_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNB40547.1"
                     /translation="MNEKTKYDKTEPTIDEGWWESVLAEENRYSTPTQRLVQSKPEVQ
                     KEAGKEFVSEEAQITVDWSQIKDLYLKDRIVELTVTGHNRGGLLVEGSGMFGFVPFSH
                     LIELAGKDNLDRSHDLEPYIGRTLHLKVIECVPEDGRVVFSERAAQTEPGKRAELFHS
                     LHAGQSVSGTVTNITDFGVFVDLGGVEGLIHISELSWGRVTHPSQFVKLGNAIDVQVL
                     DLAPERCRVALSLKRMLPNPWTNAANEFPIGSVHNATITSVLSYGVFARLDKYGVEGL
                     VHASEIPHEEGTLLKDFLSDGQAIQVRVLHLDPAHHRLGFSMRLE"
     gene            5743..>6665
                     /locus_tag="PLG52_03545"
     CDS             5743..>6665
                     /locus_tag="PLG52_03545"
                     /inference="COORDINATES: protein motif:HMM:NF036827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNB40548.1"
                     /translation="MSKPTETRRYAQEDFSPRTDWLGRLARLNTQFGRFTRDLIGVSL
                     ISFSVMAFFAIWNWTDGFVLTPIALILRTWFGWGSLLILAGIAYLGYSFLQRQQTSLH
                     WGRILALELGALLTLGLLAIPFGNDLFRAESGMDGGRLGWGIVRLLWQMGTIPGTLVL
                     LLLWALSVATGFGLWAKLEAWLIKIAGDEAPVIVSMPEPEVPQEIAVEQPVSKEAAAP
                     KKKAPAIPPEFRTQLKSAEKKPEKSLKPPPRGEDLPPLTVLMGDSTVRTDERTINQTA
                     GLIMKTLSEFGIPATVIGYRVGPSVTQFAVQP"
BASE COUNT         1575 a         1484 c         1744 g         1862 t
ORIGIN      
        1 gctgctaata aaagttttgc atcatctcct gcgctcgtga cagacaaaat gatacctgta
       61 gcaaatgtta tagccgcaat agatagactt gcaaaatcaa cttgttttag ttttttatta
      121 atgggtaaga tgatataggt ttgataaatt cctgcaatga ccatggctgc tgctgatatg
      181 tactgcctgc tttggatcag aaaaaaactg acaataaaaa gcagagttgt ggcaatcacg
      241 ccaataaggg cttcacgttt gtgcgtcagc ccccatacac aatgaatttg aattgcaaga
      301 gcggtaccaa agcaggaaaa acccagaatc cacaacggaa tgatcgagag aattttatgg
      361 gttatcccaa aatggcttgg gataataatc attcctgccc caacaccttc cagcagcaag
      421 gaaacaaaaa tgtagaaatt tttctcgtta gggcgcttca tgtcgctaga ggatagcata
      481 agaaacaaat tttatctagt tcacttcaat tgtgcatttt agggctgaaa agcccgcagg
      541 gtcatttctg ttataatcat ttcgcaaact ggtctttatg gggctgagcc gtttgtaatc
      601 ctgacgatat aaggtaaatg taaatgcaga aatttatctc tcttgctcaa atcgacgaga
      661 tcgttaatgt cataaaaaag aagatttcca ttcagccagt gatcgggatg attctcggct
      721 cagggcttaa cgggctggct gattctgtcc agaatccggt tcacattcca tacagtgacc
      781 taccaaactt ccccacctca accgtccacg gacatgttgg tcgctttgtg atcggtgaac
      841 tagaaggcaa gcccgttatc gtcatgcaag gacgaattca ttattatgaa ggctacacca
      901 tgggcgaagt aacgttgcct gtgcgcgtca tgcatcgact tggagttcat agtttatttg
      961 taaccaatgc ggctggcggt gtgaacgaga atttcgtacc aggtgatgtg atgttgatca
     1021 ctgatcagtt aaacctgatg gggatgtctg gtttgaaccc cttgatggga cccaacctcg
     1081 acgagattgg accgcgcttc ccagatatga gccagcctta tgaccgtgaa tattgtgacc
     1141 ttgcccgtaa agtggcaaag caaagtaacc taaccttgcg tgaaggggtc tacgctggtt
     1201 tgagtggacc ttcctttgaa tcaccagcag acctgcggtt cttacgcctt gccggtgcgg
     1261 atgctgtagg aatgtccaca gtaccagaag tgattattgc tcggcatggc ggaatgcgcg
     1321 tcttagggct ttcaggcgta agtaacaaag ctaatttaga tgggtccact attacaactc
     1381 acgaagaggt gattgaagct gggcgtgtga tcacacccaa gatcgaaacc atcgtacggg
     1441 gcgttcttcg cggaatataa acgcgaactt ggcaaggttg gtagatggta gacctcttta
     1501 agttcctcgc tgaatacgag gtattgattt atattgtctt ggcgattgga gcattatttt
     1561 cctttcgctg gctttttcgt tcatggcgcg aatggcaggt ggctgtcttt agtttggaac
     1621 gcgagttttc agctcggcgt tttgggcgtt ctgctttggt ctcagcgatg atcgtgattt
     1681 ttttctgcgt agagttcttc atggcatcgt tcatcatccc agggctcccc gctgatttct
     1741 ttgttgctac accgactctg gacctaatct ccacaccaac cggtacgctt tcgcccgaac
     1801 tgatgaccca atttgcaaat cagccgcaag tggcgcaacc caatgctgcc gggtgtaccc
     1861 ctgggcaaat cgagttgaca ttccctgagg ctggtactga aattaaaggc atcatcgaac
     1921 tgacaggtac tgtcgacata cccaattttg gattttataa atatgagatc gctccaagtg
     1981 gcagtgacac gtgggcgacg attgcggcag gacgtaccgt tgtgaaagca agttctcttg
     2041 ggcgttggga caccacagcg ctcacccctg gcgactatca attgagattg gtcgttatcg
     2101 ataatgaagg taattcgctt acaccttgtg tcgtgcctgt gcaggttata ccaaatcaat
     2161 aaaaggtaat ttcaattcat gtctgagtta caaattcgcc caactgttgc tactgatcta
     2221 acccgtttga tgggaattga ccattccatg accagcgaag cagtttggca gttggaagtt
     2281 cgtcgtagta cggggcaggt ggcagttaca tttcgtgagg ttcgtctacc gcgtgaaatt
     2341 caggtgccat acccgcataa tcattttgcc ttggcagatg attgggtaaa gcgctccatg
     2401 atgtatacag cgatcaagga tgccgagccg gtcggatata ttagcttgtt ggagcggggg
     2461 actgcttcaa ccgtgtgggt gctggatctg gcaattgatg cgaagcaacg tcgccgcggc
     2521 gtggctgcag ctttgttggc gtctgctcag gcatgggcgg agtcgcgatc gcatcgtcgc
     2581 ctgattctcg aagtgcagtc aaaaaatgtc ccagcgatcc ggctggcgca gaaatctgga
     2641 tttgagttct gcgggtataa tgaccagtat taccttaaca aggacgtagc actatacttt
     2701 gcgaaggtat taaagtaagg cgagcggttt taccgcccgt tgcacgattt ttattggaag
     2761 ggttgcatga ttaacggttg gccagaaaca ctactggcgg ggcttcaaac gatcaatcag
     2821 attctgacgg cgggaattgc cattacggca ttctcgttgt ttctctattc gctttcattt
     2881 aatctgcggg acagggtggc acgttcattt gcgatcattt tgttgtgtgt ggtgattgtt
     2941 tttaccgcag agtctctaca agataattca gtaactatta aggcgcttga cctgttaatg
     3001 cgcttgcaat ggtttgggct ggcgtttttg ccggcggctt acttgcatct ttcagatgct
     3061 ttgcttgtta cagctgggcg cccttctcgt ggaaggcgtc gttttgctgt gcgtggcatg
     3121 tacatcattt ctgcgctttt cctcgttttg ctcgcattcg gatatttact cggacccatt
     3181 gtgccagatg ggaaacctgc tccttacctt caacgcacag cgtggacaga ggttttcacg
     3241 atcttttatg tgacttcgat ggtctgggcg ggtgtgaatt tttaccgtgc ttatacattg
     3301 atgctaaccc gttcgggcag acgccgcatg atctacctga tggcgggtgc aaccgccccg
     3361 gctttgggct catacccgta tcttttgttt ggatattctc tcgcatcaca acatcagttc
     3421 ctcttttgga ccgctgcaac agtaatcaac gctttggttg gtgctttggt gatcgtgatg
     3481 gcgtatgcag tggcgttctt tggcgtttca tggccagacc gtgtgattaa gagccgcctc
     3541 tttaagtgga tcatgcgtgg acctgtcgtt gcctctgcta cactggcatt gatgactgtc
     3601 gtacggcgtg gtggggaact gctcggaact ccttatagtg cctttgtccc ttttacggtt
     3661 gtagcatcta ttctcttaat ggaacatgct atcacctttg ctgctccgct ttgggaacgg
     3721 ggcttgttct ttgggcgtga ccgtaatgaa ttggaattat tgcaaaacat tgaggaacgt
     3781 ctgttaaccc agggtgacct tcagcaattt ttagaagctg ttttggctgc tgtgcgcgac
     3841 catttacagt cgccttctgc ttttgttgca gccctagatg atgagacttt attaccggtt
     3901 gttgtggcgg gaaaccgtgc attgctggaa caagaaagtt tgaccgaagc tcttgaacac
     3961 atcaatggag atgtacggca cgaatttcag tggggtaatt attgggttct gccgctttat
     4021 tctcgtcgcc gacccgaaat ggatgaggat gaactcccgc ctttgttggg tctattaggt
     4081 gtggcaaaga acggcaaacg agacatcatg gagcaagacc agcgcgagtc tttgtggctt
     4141 ttagccgacc gtgccgcgtt ggcattggaa gaccgccaac ttcaacagcg ggttttccgt
     4201 tcattggcag atattcaacc tcaagtggaa atgatccaac ggatgcgtgc ggcgagccgt
     4261 tatgatacca acgccaattt attgacggcg cctttgcccc gtgaagctga ccttgcgaat
     4321 tgggtcaaag atgcgcttac gcattattgg ggtggaccca agttcacgaa taatccactt
     4381 acgaagttgc aggtggtgca agaactggca gacaaggaag atggaaatat ctcaaacgcc
     4441 ttgcgaactt tgttgcgtca ggcagtggat gaagtcaaac cgcgtggaga tcgtaaattc
     4501 accactgagt ggatcttgta caatatcctt gaaatgaaat ttattgaagg gcgtaaagtc
     4561 cgtgatgtgg cggcgcggct tgccatgtct gaagcggatt tatatcgcaa acagcgtgtg
     4621 gcaattgaag cggttgccaa ggcaattctc gagatggaag tgagcaagct cgagcagtaa
     4681 gttttttatt ggaagatctc gtcgaagaat cctgatgtat ctctaggagg gcgcattggg
     4741 atttagggtg cggcgattta taaggagaat gttatgaatg aaaaaaccaa gtacgacaaa
     4801 acagaaccaa cgatcgatga aggctggtgg gagtcggtcc ttgcggagga gaacaggtac
     4861 tcgacgccga cacagcgtct agtgcagtcc aagcccgagg tccagaaaga ggcgggcaag
     4921 gagtttgttt ctgaagaagc tcaaatcaca gtcgattgga gtcagatcaa ggacttgtac
     4981 ttaaaggatc gtatcgttga actgaccgtc accgggcata atcggggtgg tttattggtg
     5041 gaaggcagcg ggatgtttgg ctttgtccca ttttcacatt tgatcgaact ggcaggcaaa
     5101 gataatcttg accgcagtca cgatcttgaa ccttatattg gacgtactct ccacttgaaa
     5161 gtgattgaat gtgttccaga agatggacgt gtggtttttt cggaacgtgc tgctcagacg
     5221 gaacccggaa aacgcgctga gttattccac tcattgcatg caggtcaaag cgtaagtgga
     5281 acagttacga acattacaga ctttggtgtg ttcgtagacc ttggcggtgt ggaaggtttg
     5341 attcatatct ctgaactttc ttgggggcgt gtaacgcacc cctcgcaatt tgtaaaactt
     5401 ggcaatgcca ttgatgtgca agtattagac cttgcgccag aacgctgccg tgttgcctta
     5461 agtttgaagc gtatgcttcc caacccttgg acgaatgcgg caaatgaatt cccgattggt
     5521 tctgtgcata atgcaaccat caccagcgtt ttatcttatg gcgtgtttgc acggctggat
     5581 aaatatggtg tggaaggctt ggttcatgct tctgaaattc cgcatgaaga aggcacattg
     5641 ctaaaagact ttctctctga cgggcaagcg attcaggtgc gtgtattgca tctcgaccct
     5701 gcacatcacc gccttggctt cagcatgagg cttgaataag ggatgtctaa acccactgaa
     5761 acaaggcgtt acgctcaaga agatttttct ccgcgcaccg attggctcgg aaggcttgcg
     5821 cgtcttaaca cgcaatttgg gcgtttcact cgcgatctta ttggtgtaag cctaatctcg
     5881 ttttcagtca tggcattctt tgccatttgg aactggaccg atggctttgt gcttacacca
     5941 atcgccttga tattgcgcac ttggtttggg tggggcagtc tcctgatttt ggcagggatt
     6001 gcttatcttg gatattcctt tttacaacgc caacagacgt ctctacactg ggggcggatt
     6061 cttgctcttg aactcggcgc tctcttaacc ctgggcttgc ttgctatacc ctttggcaac
     6121 gacctcttcc gagccgaatc tggcatggac ggtggaaggt tggggtgggg tattgtgcgc
     6181 ctgctgtggc aaatgggaac cattcccggc acgttggtgc ttttgctttt gtgggcgttg
     6241 agtgttgcta ctgggtttgg gctgtgggca aagcttgaag catggctcat aaaaattgca
     6301 ggtgacgaag cgcctgtgat cgtctctatg ccagagccag aagttccaca agagatcgct
     6361 gtcgaacagc cagtttcaaa agaagctgct gctccgaaaa agaaagctcc tgccattccg
     6421 cctgagtttc gtactcagtt gaaatctgct gaaaagaagc ccgagaaatc actcaagccg
     6481 cctccacgcg gtgaagattt accgcctctc actgtcttga tgggcgactc aactgtccgc
     6541 accgatgaac gcacgatcaa ccaaactgcc ggtttgatca tgaaaactct ttcagagttc
     6601 ggtattcccg caacggtaat cgggtatcga gttggtccat ctgtgacgca gttcgctgtt
     6661 cagcc
//
LOCUS       DAOQNJ010000075         6563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6542_length_6563_cov_8.260295, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000075 DAOQNJ010000000
VERSION     DAOQNJ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6542_length_6563_cov_8.260295"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="PLG52_03550"
     CDS             <1..170
                     /locus_tag="PLG52_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB40549.1"
                     /translation="AELDSAEDMVVFCKRGSRSARAIEILSSAGFKKMKNLKGGINAW
                     AEEVDKSLPLY"
     gene            complement(233..1798)
                     /locus_tag="PLG52_03555"
     CDS             complement(233..1798)
                     /locus_tag="PLG52_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB40550.1"
                     /translation="MSRKIIPFVIALILICLVMGVAGVFAFSGFAAAEKFGSQTAWSQ
                     SYSQAESMKVIDVTGDGQDDLFIQNASEVNILDGSGTPILGLDYPSPKTTLGDVNGDG
                     VEDIVVYYMGPEGASVDVISKGSVSNLAKSLNIGSPARVAFIRFSSGPQIVLGDSSGN
                     VLSIDAGSGAPLWNSNVGSAEIRGMDDARIGGEVHVAIASNDGSVKIFASDGSNVWTV
                     NQEQLRRMRAFDLNADGNSEVITGGEYGAFKIYNAANGQVLFEKSIGQAVSEVREVEL
                     NGDPSSREIVVGGKDGGVWAYSFNGTTASQLWTGSLSDKVTEIAGLDINEDGKQEAVI
                     GDDSGKVAVFTDNGTRNNLPDHSTGITRIDIGKLGSERYVVIADYNEVQTNKVNFSSI
                     PGFQFTPLIVGIIVSAVILAIAGILASIPPKPEMKLSLQDKSRESLEAERRMLKESIA
                     DVERLRKAGEMSNEAYLARLKRLRGDLAENEAAFKTANFPIKTETFACPNCGGTLELG
                     MDKCEYCGQVILS"
     gene            complement(1799..2749)
                     /locus_tag="PLG52_03560"
     CDS             complement(1799..2749)
                     /locus_tag="PLG52_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40551.1"
                     /translation="MDGLICGLIIAGIVVISFFFYLLNTARKKGNTDKNQFGKTEYSP
                     VYVSIADKPDSIIRGMNKFVAEARKTETAGDKWRWIPMLIFFAGIGLALIDVFILLLG
                     YSISFVFIGGGVLLWIAAFVMARALRRSDLLDFSPRYNGTKEILHTLRDDLKPGSTFL
                     GHLDLTGSMLQNKVARETQDAQSRTTQLFNDPWLSLKAKLYDGNVLRVTAIQKTKKRK
                     SYWKRSRISGKSKLKPEKFKGSQQELKVRIVVNPEAYVIRPNNQFSLNKDVGKYKVAQ
                     LSTEGGMINILAVSPFEDVSHTDILTFLKSAYSLLERKAA"
     gene            2817..3980
                     /locus_tag="PLG52_03565"
     CDS             2817..3980
                     /locus_tag="PLG52_03565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB40552.1"
                     /translation="MKNVLYTAFDIVPSPKGASTHILHNLRGLVNGNFNVHLITPNDG
                     VLPPEDTIEGARLTRISQDLSQNFLARAVHFGKSVLSHLALNPGYDVVHYRNIWDGFH
                     ITQNKKKFGYKTIFEVNGLSSIELKYHYPGMDASLLAKIKEQELATLHLSDAIICPSN
                     VTRDYIASLGLNRKLVTVIPNGVSPSDFPVTPLPIRDGREPVLLYIGTLADWQGLDIV
                     IKALPKILEQQPVKLRILGRGRSRQRKMLAKQIRKLGLDEYVLVQPAVPHHEIPAVIA
                     SADLCVAPLGLNDRNVTQGACPIKVLEYMAAGRPLLASNMPIVRELVREDVDGLLFSP
                     NDPDDLARQANQLLKDMELSKRLAESAAGYVREKFTWHESQKKLLKVYEKLIK"
     gene            4048..5052
                     /locus_tag="PLG52_03570"
     CDS             4048..5052
                     /locus_tag="PLG52_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40553.1"
                     /translation="MLDFIKEAGFPAIALLLGGVMVVIAIIRNIALEKVKIQLTSSQS
                     IQLAVVGVLFIIGGFVLQYFPPSSANTPENVDVYSTLYALQTQMVQPVNPQGQTNQTS
                     TPAGFALVSPTVTLFPNPVATLTPTLIPFTLTPDPSQPLAEVFCTNFYSIMVREGPND
                     NYPIKSVIENPNNLSNPDCMLFDFRMPDNSWIRIAANQDNPNYTQHELGWVTSDQFRP
                     IDFDKLRVYIPENVQDGLYCVTSRYGLKVRSCPNEGCREVGNLSLQDCLFMDGRSSDG
                     LWVRVSADKNDEKYASYKGNWVSTYFLAPFEFSAGYQPYFRYYFELLPTVEQIPTPNG
                     "
     gene            complement(5092..5496)
                     /locus_tag="PLG52_03575"
     CDS             complement(5092..5496)
                     /locus_tag="PLG52_03575"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB40554.1"
                     /translation="MPISSIYSKTITIPSSAIDENGHVNNVVYVQWMQDIAIEHYASI
                     GGVEAQGPDATWVIRKHSIEYFLPAFEGEEIEVRTWVEDLRKVRSLRMYEFVRKSDGK
                     TLVKGESDWVFMDMKTGRPKAIPAEVENIFKP"
     gene            5596..6015
                     /locus_tag="PLG52_03580"
     CDS             5596..6015
                     /locus_tag="PLG52_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40555.1"
                     /translation="MGPIIGGTSLIITVISIACGCLITIVTLAGTGFLIYKVFGSTFK
                     SMSTNRNVLQTGVSAPAVILSVQDTGVTMNDNPQARIRLRVMPMNGEAFEAEVTQIIG
                     RFQVGMVVPNGSAMVKYDPNDKTKVAIESFGMPSAGQ"
     gene            complement(6064..>6563)
                     /locus_tag="PLG52_03585"
     CDS             complement(6064..>6563)
                     /locus_tag="PLG52_03585"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB40556.1"
                     /translation="AVETYASNLTQLAPTSTPFPTLTPVDITATALSLTPEPSPTSNP
                     CFNLLYIDDVTVPDGTRMKPGEKFTKTWLVQNIGGCAWRAGFTFQNVGGDSMRGTPIT
                     LTEAIQTGAKRELSVELVVPSGINGVIESAWQMADDTGVFFGDTLFVSIVVGDVTTPA
                     VTSTP"
BASE COUNT         1686 a         1577 c         1522 g         1778 t
ORIGIN      
        1 tggcagaatt agactccgct gaagacatgg tcgtcttctg caaacgcgga tcccgttccg
       61 cgcgtgcgat tgaaattctt tcctccgctg gcttcaagaa gatgaagaac ctcaaaggcg
      121 gcatcaatgc ctgggctgag gaagtagaca agtctctgcc gttgtactaa caagaaataa
      181 aaaaacatcc gccaatattg gcggatgttt ttttatttac aatcctactt tcttacgaca
      241 aaataacctg accgcaatac tcgcacttat ccattcccaa ttccaaggtg ccgccacagt
      301 tggggcaggc gaaggtttcg gttttgatgg ggaagttcgc ggtcttgaag gcggcttcat
      361 tctcagctaa atctccgcgt aggcgtttga ggcgcgccag ataagcttca ttcgacattt
      421 cacccgcttt gcgcagacgt tccacatccg caatcgattc tttgagcatg cggcgttcgg
      481 cttcaaggct ttcgcggctc ttatcctgaa gcgagagttt catctctggc ttgggcggaa
      541 tagaagcaag aatgccagca atcgccaaaa taacagccga gacaatgatg ccaacaatca
      601 acggtgtaaa ttggaaaccg gggatcgagc tgaagttcac tttgttcgtt tgcacttcat
      661 tgtaatcagc gatcaccaca taacgctcac tgccaagttt gccaatgtcg atgcgggtga
      721 taccagtgga atggtcgggc aggttgttgc gcgtaccatt atctgtgaag actgccacct
      781 tgcctgagtc gtcgccgatg accgcttctt gcttaccatc ttcgttgatg tctaagccag
      841 cgatctcggt caccttgtct gataaagacc cggtccacaa ttgcgaagcg gttgtgccat
      901 tgaacgaata cgcccacaca ccaccgtctt tgccgcctac tacgatctcg cgcgaagatg
      961 ggtcaccatt caactccact tcgcgaactt cgctcacagc ttgcccaatg gacttttcaa
     1021 agagaacttg tccatttgcg gcgttgtaga ttttgaaggc gccatattca ccacctgtga
     1081 tgacttcgct attgccatcg gcattcaggt cgaatgcacg catccttctt aattgttcct
     1141 gattcaccgt ccaaacatta ctgccatctg aagcaaagat cttcaccgaa ccgtcgttcg
     1201 atgcaatcgc cacatggact tctccgccaa tacgggcatc gtccatgccg cggatttctg
     1261 ccgagccgac gtttgaattc caaagcggcg caccgctccc agcatcaatc gaaaggacgt
     1321 ttccgctgct gtcgccgaga acgatctgag gtccgctaga gaagcgaatg aatgccacac
     1381 gggcagggga gccgatgtta agcgatttcg ccaggttgct tacactgccc ttggagatta
     1441 catcgacaga agcgccttcg ggacccatat agtaaacaac aatgtcttcc accccatcac
     1501 cgttgacatc tccaagagtg gtcttggggg aggggtaatc cagcccgaga atgggtgtac
     1561 cagacccatc gaggatattg acctcgcttg cgttttgaat aaagaggtca tcctgaccat
     1621 caccggtcac atcaatgacc ttcatgcttt ctgcttgcga ataggattga gaccatgcgg
     1681 tttgactgcc aaatttttcg gcggcggcaa atccgctaaa agcgaacacg ccagcaaccc
     1741 ccatgacaag acagatcaag attaatgcaa tgacgaacgg aataattttg cgactcattt
     1801 atgcagcctt tctttccaat aatgaataag cagatttgag gaaggtcaaa atatcagtat
     1861 gactgacatc ttcaaaagga gaaacagcaa ggatgttgat catgccgcct tcggtgctga
     1921 gctgagcgac cttatacttg ccaacatctt tatttagcga aaattgattg ttcgggcgaa
     1981 tgacatacgc ctcggggttg accacaatgc gcactttcaa ttcctgttgt gagcccttga
     2041 acttctcagg ctttaacttg gatttgccgc tgatgcgact gcgcttccaa tacgatttgc
     2101 gttttttggt cttttgaatg gcagtaacac gaagcacatt gccatcatac aacttggctt
     2161 tgagcgaaag ccaagggtcg ttaaaaagtt gggttgtgcg gctttgagca tcttgtgttt
     2221 cgcgggctac tttattttgc aacatggaac ccgtcaggtc gagatgccca aggaatgtag
     2281 agccaggttt gaggtcgtct cgaagcgtgt gcagaatttc ttttgtcccg ttatagcgcg
     2341 gagagaaatc caacaagtca ctgcggcgta aggcacgtgc catcacaaag gctgcgatcc
     2401 aaagtagaac accgccgcca atgaagacaa aggagatgct atacccaagt agtaagataa
     2461 aaacatcaat gagtgccagc ccaatgcccg caaagaagat cagcatcgga atccaacgcc
     2521 acttatcccc ggctgtttcg gtctttcgcg cttcagccac aaacttgttc attccgcgaa
     2581 tgatcgagtc tggcttgtct gcaatcgaaa catagacagg tgaatactct gttttgccga
     2641 attgattttt gtcggtgttt ccttttttac gtgcggtgtt cagcaggtaa aagaaaaaag
     2701 agatgacaac gatccccgcg atgataagcc cacaaatcag accatccatc catgcctccg
     2761 agttattttt atgcccacag tcgcaatttg tgaccgcgct gggtataatt tcttgaatga
     2821 aaaacgttct ctacaccgca ttcgatatcg tgccaagccc gaagggtgcc agcacccata
     2881 ttctccacaa tttgcgaggg ttggtcaatg ggaactttaa tgtccatctt atcacgccca
     2941 acgatggcgt gctccccccc gaagatacta ttgaaggcgc cagactcacg cgcatttcgc
     3001 aagacctttc acagaatttc cttgcccgtg ccgtgcattt tggtaagtca gttttatcgc
     3061 acctagccct caaccctggc tatgatgttg tccactatcg caatatttgg gatggctttc
     3121 acatcacaca gaataaaaag aagtttgggt ataaaactat ttttgaggtg aatgggcttt
     3181 cttccattga actcaaatat cattaccccg gtatggatgc cagcctgctc gccaagatca
     3241 aagaacagga attggcgact ctgcacctga gcgacgccat catctgccca tcaaacgtga
     3301 cgcgcgatta catcgcctcg ctcggtctta accgcaaact ggtgactgtt atcccgaacg
     3361 gagtcagccc ttcagacttt cccgttaccc ctttgcccat tcgagatgga cgcgagccgg
     3421 ttttacttta catcggtaca ttagccgatt ggcaaggctt ggatatcgtc atcaaagccc
     3481 tgccgaaaat ccttgagcaa cagcctgtca aactccgcat cctcgggcgc ggacgttccc
     3541 gtcagcgtaa gatgttggca aagcaaattc gcaaactggg tttggatgaa tatgttttgg
     3601 tgcaacctgc tgtaccgcat catgagattc cagctgtgat cgccagtgca gatctttgtg
     3661 ttgcaccact cgggttgaat gaccgcaatg tgactcaagg tgcttgcccg atcaaagtgc
     3721 ttgagtatat ggcggctggg cgacctttac tcgcctcaaa tatgcccatt gtccgcgaac
     3781 tggtgcgcga agatgtagac ggattgctct tttcacccaa cgacccagac gacctagcgc
     3841 gacaggcaaa tcaattactg aaagatatgg aactatccaa acgcttggct gagtctgcgg
     3901 cgggatacgt ccgcgagaaa tttacatggc acgagtcaca gaagaaattg ttgaaagtgt
     3961 acgaaaaact aataaagtaa cagaccttct tttttgttga aaatccgccc cggtttcagt
     4021 atataatttt cgaataggag gagcaacatg ttagatttca ttaaagaagc cggttttccc
     4081 gccattgctt tgttgctcgg aggggtaatg gttgtaattg ctatcatcag aaacattgcc
     4141 cttgaaaaag taaaaataca acttacttca agccagtcta ttcagttggc agtggtgggt
     4201 gtattgttca ttataggtgg gttcgtttta cagtattttc ctccttcttc tgcaaatacc
     4261 cctgaaaatg ttgatgttta ttccacgctt tatgccttac agacccaaat ggtgcagcct
     4321 gtaaacccac aaggacaaac taatcagact tctacacctg ctggttttgc tttggtttcg
     4381 ccaaccgtta ccttgtttcc aaacccagtg gcgaccctaa caccaacctt aatccccttc
     4441 acactaactc cagatccttc acaaccatta gcagaagtgt tttgcacgaa tttttatagc
     4501 attatggtac gtgaaggacc taatgataat tacccgatca agtctgttat tgagaacccc
     4561 aataatttga gtaacccaga ttgtatgctt ttcgattttc gcatgccaga taactcatgg
     4621 atacgtattg ctgcaaatca ggataacccc aattacaccc aacatgaact tggttgggtg
     4681 acttcagatc aattccgccc gattgatttt gataaattgc gagtatatat ccctgaaaat
     4741 gtgcaagatg gtttgtattg tgtcaccagc cgatatggtt tgaaagtgcg gagttgcccc
     4801 aatgagggtt gtcgtgaagt tggcaacctg tctttgcaag actgtctttt catggatggg
     4861 cgttcttcag atggtttatg ggtgagagtt tcagccgata aaaatgatga gaaatatgct
     4921 tcttataaag gaaattgggt gagcacttat ttcttagccc cctttgaatt ttcggctggg
     4981 tatcaaccat atttccggta ttactttgaa ttattaccta ctgttgaaca aatcccaacc
     5041 ccgaatggat aacgaagaac caggtcacgc ttcaaacgtg acctggttct tttatggctt
     5101 gaatatattt tctacttcgg cgggaattgc ttttgggcgt cctgttttca tatccataaa
     5161 aacccagtca ctttcgcctt ttactaaagt cttcccatca ctcttgcgga caaattcata
     5221 catgcgcaac gaccgtactt tgcgtaaatc ctctacccaa gtgcggacct caatctcttc
     5281 gccttcgaag gctggtagaa aatattcgat gctatgttta cggatcaccc aggtggcgtc
     5341 aggtccctgg gcttctacgc ctccgatgga tgcataatgt tcaatggcaa tatcctgcat
     5401 ccattgcaca tagactacgt tattgacatg tccattttcg tcgatagcag aagaggggat
     5461 tgtgattgtt ttggaataaa tagaggaaat cggcattttt tgattttaca gtgacttccc
     5521 ccaaacgggg gagtttattt ttattgcccc gctgatagaa tgaatttaat aaaaatcatt
     5581 ttttggagaa attaaatggg accaatcatt ggtggcactt cattaataat tactgtaatt
     5641 tcaatcgctt gtggatgttt gattaccatt gtcacactgg ctggaactgg ttttctgatc
     5701 tacaaggtat ttggaagtac gttcaagagc atgtcaacaa atcgcaatgt tcttcagact
     5761 ggcgtttctg ctccggcggt gattctttct gtgcaagaca cgggtgtgac catgaacgat
     5821 aatccacagg ctcgcatccg tttgcgtgtg atgccgatga acggcgaagc atttgaagca
     5881 gaagttaccc agatcatagg gcgttttcag gtgggaatgg tcgttccgaa tggctcggca
     5941 atggttaaat atgacccgaa cgataaaacc aaagtagcaa ttgagtcctt cggtatgcca
     6001 tcagccgggc aataattcta gaagaaacaa aaaggcgtgg attttatcca cgcctttttg
     6061 tttttacggt gtacttgtca cggctggagt ggttacgtca cctacgacga tgctgacaaa
     6121 caatgtatcg ccaaagaaga caccggtatc gtctgccatt tgccaggcgc tttcgatgac
     6181 accattaatg ccactaggca caacaagttc tacagaaagt tcgcgtttag cgccggtttg
     6241 gatggcttcg gtaagagtga tgggagttcc acgcatagag tcgccgccta cgttttggaa
     6301 ggtgaaacca gcacgccagg cacaaccacc aatgttttgc accaaccatg ttttagtaaa
     6361 tttttcgccg ggtttcatcc gtgtcccatc tgggacggtg acatcgtcaa tgtacaacag
     6421 gttgaagcag ggattcgatg tgggggatgg ctcaggggtg agggaaagcg ccgttgctgt
     6481 gatgtcaaca ggcgtcagtg taggaaatgg tgtggaggtt ggagcaagct gggtcaggtt
     6541 tgaggcgtat gtttccacgg ctt
//
LOCUS       DAOQNJ010000076         6511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6623_length_6511_cov_9.367255, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000076 DAOQNJ010000000
VERSION     DAOQNJ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6623_length_6511_cov_9.367255"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..775)
                     /locus_tag="PLG52_03590"
     CDS             complement(2..775)
                     /locus_tag="PLG52_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB40557.1"
                     /translation="MTYTTILTETRGRVGIVQLNRPQAMNAFNLAMLGEVFDALEVFD
                     KDENVGAIIVTGNEKAFAAGADIKEMADQTYVQMLANEHVKIWDRIRGIRKPVIAAVS
                     GWALGGGSEFVLSCDMVIASESAKFGQPEITIGIIPGAGGTQRLARLVGKHLAMEMVI
                     NNRTLTAQEAQQFGLANHVVPVEGYLDAAVAFAEEIASRAPIAVRMAKDAVNAAFETT
                     LTEGLAVEKRNFYPLFATEDQKEGMQAFIEKRKPNWKGK"
     gene            917..1315
                     /locus_tag="PLG52_03595"
     CDS             917..1315
                     /locus_tag="PLG52_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40558.1"
                     /translation="MQILFLIHSHMRWLILIVALVAIVKFAIGWLRGGAFKGMDRGLA
                     SGFSGLMDLQATLGLIFLIWTGLAGVGFPMNRIEHATTMIIAAVLAHLPARWKNASDT
                     VRFRNTLFCIVGSLVLVFMGVVRLRGGWTW"
     gene            1364..1957
                     /locus_tag="PLG52_03600"
     CDS             1364..1957
                     /locus_tag="PLG52_03600"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNB40559.1"
                     /translation="MKKLILIGPVLFAVVLVTLTFVKYDFLLSLGWHPINDPTFDWPS
                     GLALGRFGWIMTATFITSGLIMTLFARRLFLDLKPGTASKLGTTLMAFAGLFLATLAF
                     TTDPTIRDTPATWHGQLHDLSFVLLGLTLFPAMIVLGFAFRADERWKNLAVYTWGTLA
                     LAGPAFFIKGAAFYVFLFAILAWNEIAAIRLNSHAEN"
     gene            complement(1992..4673)
                     /locus_tag="PLG52_03605"
     CDS             complement(1992..4673)
                     /locus_tag="PLG52_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB40560.1"
                     /translation="MLSENSELAHQGSTEVQRARKTARSTFFTALKFMATRLLTVLLT
                     IGLGTFITIVAANGGGAIDNAEKSRINAVTLRSVDPFATSAEELTKLRADMEEKEGLN
                     LPFLPRHMYWTYKALTLNWGEVNDRVKFHPRYTTSNMNAEDSRVILMQYLPNTILLIG
                     AGFFFLFIAGIPFALHLSQNPGGRLDKLIGTLTPLSSIPGWVIGFILILVFAVGLKIL
                     PVGRMIDSIPPESSFEYVLTVIRHMVLPVTAILLSSAFTLIFSWRTYFLAYSHEDYVD
                     VGKAMGLPQKTFRRNYILRPVLPAIITSFSLSMVGFWQLITTVEFVFDWPGIGSLFVR
                     ALPHFLGERMYPGEIPIIIGTMVIFAYLLGFTVFMLDIAYLVTDPRIRVTSGGSSQRL
                     VAKRTKRKWTFPTFKRPEFSWTETKQNFSDTKAQWAENWKKFLALLSELRGYPSAIIG
                     FSIVGLLVLTSIYTVIALPYRQIGRDWFLLSVKPRSVVPKLALPSWVNFFRENDLPST
                     ILIDSRTDSVERTETVLNETTKTITLTYTFDYPYTEFPKDMVIYYYPTYQEKVPFASM
                     ILTTPDGRELELRGEMTSFEKNYEPGSVLNINKILKQNPNWKEWFSIDEHYPTPAFYL
                     LFADPNAKTAKAVPGTYILTINAVGFEKDWDLDAQLVIFGQVYGMAGTDYMRRDLMTP
                     LLWGMPIALIFGLLGSIVITVGSAIVGAAAAWYGGWVDKALQVVIEANMILPVLAVGI
                     LLYAFYQVNFWLILMIIILLNIFGSPTKTFRAAFLQVKEASYVEAARAYGATNWRIIW
                     HYLIPRITPVMVPQLIALIPSFAFLEATFAIFNVSEVMYPTWGLVIQNAMKYGAAYGS
                     QFWVLEPISLLLLTGVAFGMSSAAFDYILNPRLRTKG"
     gene            4796..5371
                     /locus_tag="PLG52_03610"
     CDS             4796..5371
                     /locus_tag="PLG52_03610"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNB40561.1"
                     /translation="MHLEQLITHFGYPALLLGLLLEGETVLVLAAFMAHRGYLDLPLV
                     ILLGGLVAFASDQFFFWMGRIQGNTFLANRPAWQPHVEKAKALLDRNSTLLFLSVRFM
                     YGLRTVLPFVLGMSKLNTKKFVLLDFIGAFLWAITFGLAGHFIGHVMTLIFDDVKEHE
                     LPVMIGIALIGIGIWLYRRNNNKDKDGKYHE"
     gene            5364..5918
                     /locus_tag="PLG52_03615"
     CDS             5364..5918
                     /locus_tag="PLG52_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNB40562.1"
                     /translation="MNNHSQEQFKNLFLEKMETFTRNEWELLRHIMDRRHVTRNVNKE
                     FDDQLTFGQRLADNVAKFGGSWTFIIIFGLILLTWVFLNSVVLARLNDAFDPYPYILL
                     NLFLSMLASIQAPIIMMSQNRQATKDRMDAAHDYEVNLKAEMEIETLHQKLDELRDKQ
                     WAELVEMQQEQIRLLTKLLEEKNK"
     gene            6171..>6511
                     /locus_tag="PLG52_03620"
     CDS             6171..>6511
                     /locus_tag="PLG52_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40563.1"
                     /translation="MRIAKHFRKDQETLQRFLDVFGAGSAMLGGGNKNVQPGFFIHGS
                     TFIHEYVDGNFFRKEELLMKTLEDAGFPVNSGVIGNMKDEHAKCRQAADLLSKAASDW
                     QGGDAGARIEVG"
BASE COUNT         1637 a         1470 c         1642 g         1762 t
ORIGIN      
        1 gctacttacc tttccaattc ggtttgcgtt tttcaatgaa ggcttgcatg ccttcctttt
       61 gatcttcggt cgcaaataat ggatagaagt tacgcttctc gacggctaga ccttcggtga
      121 gtgtggtttc aaaggcagca ttgacggcat ctttcgccat gcggacggcg atgggtgcgc
      181 gcgaagcaat ttcttccgca aaggcaacag cagcatcgag atacccttca acaggcacaa
      241 catgattcgc gagaccgaat tgctgagctt cttgtgcggt gagggtgcgg ttgttgatga
      301 ccatttccat ggcgagatgt ttgcccacga gacgagccag tcgctgtgtg ccgcctgcgc
      361 cggggataat gccaatagta atttcaggct gaccgaactt tgcagactcg ctcgcgatga
      421 ccatatcgca ggaaagcacg aattcgcttc cgccgccgag cgcccaaccc gaaactgctg
      481 caatgaccgg tttacgaatg ccgcgaatgc gatcccaaat ctttacatgt tcattggcaa
      541 gcatttgcac ataggtttga tctgccattt ctttgatatc ggctccggca gcgaaggctt
      601 tctcattgcc ggtgacgatg atcgcgccaa cattttcatc tttatcaaaa acttccaatg
      661 catcaaagac ttcaccaagc atggcaaggt tgaacgcgtt catggcttgc gggcggttca
      721 actgcacaat gcctacgcgt ccgcgggttt cggtcagaat ggtggtgtag gtcatgggtt
      781 cctccaaagt tcagatagtg agattataaa ctcaacctgc actgggtata attctcaaac
      841 cctattgggc agcgtaagaa cggctatatc cgcttcatcc caaatatctg aactaaattt
      901 atatacggag actctcatgc aaattctatt cctcattcat tcccacatgc gctggttgat
      961 cttgatcgtg gcgttggttg ccatcgtcaa atttgccatt ggctggctgc gtggcggcgc
     1021 cttcaaaggc atggaccgcg gtctggcttc tggttttagc gggctgatgg acttacaagc
     1081 cacgctcggc ttgattttct tgatctggac cggcttggca ggcgttggct tccccatgaa
     1141 tcgcatcgaa catgccacca ccatgatcat tgcggcagtg cttgctcatt tgcccgcccg
     1201 ttggaagaac gcctcagaca ccgtccgctt ccgcaatacc ttgttctgta ttgtcggttc
     1261 gttggtgttg gtcttcatgg gtgtcgtccg cttgcgcggc ggttggactt ggtaatctaa
     1321 aaaagggaac agcctcgcgt atgcgaggct gttttgttta aacatgaaaa aattaattct
     1381 tattggtccc gttctctttg cagttgtgct tgttacgctg acattcgtca aatacgattt
     1441 tcttttgagt ttgggctggc atcccatcaa cgaccccacc ttcgactggc ccagtgggct
     1501 ggcgctcggt cgcttcgggt ggatcatgac cgccaccttc atcaccagcg gactcatcat
     1561 gactcttttc gcccgtcgcc tctttctgga cttgaagccg ggcacagctt ccaaacttgg
     1621 caccacgctg atggcgttcg ctggtttatt tctcgccacc ttggctttca ccaccgatcc
     1681 aaccattcgt gatactcccg ccacttggca tggacagctg catgacctct cgtttgtgtt
     1741 gcttggacta acgcttttcc ccgcgatgat cgtccttggc tttgccttcc gcgccgatga
     1801 gcgttggaag aatcttgccg tctatacgtg ggggacgctt gccttggcag gaccagcatt
     1861 ctttatcaaa ggcgcagctt tctatgtttt tctattcgca attttggcat ggaatgaaat
     1921 tgcggcaatt cgtttgaact cacatgcaga aaactaacag cgtaacgaaa agccagagaa
     1981 actgaatcaa cttatccctt tgttctgagg cgcgggttga gaatatagtc aaatgccgcg
     2041 ctagacattc caaatgcaac accggtcaat aagagcaaag agattggttc caagacccag
     2101 aattgtgagc cgtaggcggc gccgtatttc atcgcatttt gaatgaccaa gccccatgtg
     2161 gggtacatta cttcagaaac gttgaagatg gcgaaggtgg cttcgagaaa agcgaaacta
     2221 ggaatgagag caataagttg cggcaccatc accggtgtga tgcgcgggat gaggtagtgc
     2281 caaatgatgc gccaattggt tgcgccatat gcacgcgcgg cttctacgta cgaagcttct
     2341 ttgacttgta aaaacgcagc gcggaaggtt ttggtggggc tgccgaaaat atttagcaag
     2401 atgatgatca ttaagatcaa ccaaaagttg acctggtaaa aggcgtataa caaaatgcca
     2461 actgccagaa ccggcagaat catattagct tcgatcacca cttgcagggc tttatctacc
     2521 cagccgccat accaagcggc ggctgcgcct acaatagcag acccaaccgt gatgacgatg
     2581 gaacctagca acccaaagat gagggcaatg ggcatgcccc acaaaagcgg agtcatcaga
     2641 tcacggcgca tgtaatcggt accagccatg ccatacactt gtccaaagat caccaactgg
     2701 gcatcgaggt cccagtcttt ttcgaagcca accgcattga tggttaggat gtatgtgccg
     2761 ggtactgcct tggcagtttt ggcattgggg tctgcaaata ataaatagaa agcaggtgtg
     2821 gggtaatgct catcgatcga gaaccattct ttccagtttg ggttttgctt gagaatctta
     2881 ttgatattga gcacactgcc cggttcgtaa ttcttttcaa aggacgtcat ctcgccacga
     2941 agttccagtt cgcgcccatc gggtgtggtg aggatcatcg atgcaaaggg aactttctct
     3001 tgataggtcg ggtaataata aatgaccata tcttttggaa attctgtgta aggataatca
     3061 aaggtgtagg taagtgtgat ggttttggtc gtttcgttga gtacggtttc tgtgcgttcg
     3121 acagaatcgg tgcggctatc tatgagaata gtggatggca ggtcgttttc gcgaaagaag
     3181 ttgacccaac ttggcaaagc cagtttggga accacacttc tcggtttgac gcttaataag
     3241 aaccaatctc tgccgatctg acgatagggc aaggcgatga cagtgtagat ggaggtcaat
     3301 accagaagac caacgatgct aaagccaatg atcgcagagg gatatccgcg tagttcactt
     3361 aagagcgcaa ggaatttctt ccaattctct gcccattgcg cttttgtatc ggagaaattt
     3421 tgttttgttt ctgtccatga gaactctggt cgtttgaagg ttgggaatgt ccacttacgc
     3481 ttggtgcgtt tggcaaccag cctttgtgag cttccaccag atgtgacgcg gatacgtgga
     3541 tctgtgacta gataggcaat atccaacata aagacggtga agcccaacaa gtaggcaaaa
     3601 atgaccatcg tgccgatgat gatgggaatt tcaccggggt acattctttc acctaaaaag
     3661 tgaggcaagg cgcgtacgaa tagtgagccg atgccgggcc aatcgaagac gaattccaca
     3721 gtggtgatca actgccaaaa tcccaccatg gaaagggaga aactggtgat gatggcgggc
     3781 aataccgggc gcaggatgta atttcgtcgg aaagtctttt gcggaagacc catggctttt
     3841 cctacatcta cataatcttc gtgagagtaa gcgaggaagt aggtacgcca ggaaaagatc
     3901 aatgtgaatg cgctggaaag caaaatggct gtaaccggta agaccatgtg tctgataacg
     3961 gtgagcacgt attcaaagga tgattcaggt ggaatgctgt ctatcatccg cccgacaggg
     4021 aggatcttta aacccacagc gaagactaga atcaaaatga aaccaatgac ccagcccgga
     4081 atggaggaaa gaggcgtgag cgtccctatg agtttgtcta accgcccacc ggggttttga
     4141 gataggtgta gcgcaaatgg aatccctgca ataaaaagaa agaagaagcc agcaccgatc
     4201 aaaaggatgg tgttaggaag gtattgcatc aggatcacgc ggctatcttc tgcattcata
     4261 ttggaagttg tgtagcgcgg atgaaacttc actctgtcgt ttacttctcc ccagttcaga
     4321 gtcagtgctt tatacgtcca gtacatgtgg cgcggcagaa aggggagatt taacccttct
     4381 ttttcttcca tatctgctcg tagtttagta agctcttcgg cagatgtggc gaaagggtcc
     4441 acactccgca aggtgacggc attaatgcga ctcttttctg cgttgtctat ggcaccgcca
     4501 ccattggcag ccacaatggt aatgaatgtg cccaacccaa tggtgagaag gaccgttaac
     4561 aatcttgtcg ccatgaactt cagggcggtg aagaacgtac tccgagctgt tttgcgtgct
     4621 ctttgtacct ctgtacttcc ctgatgggca agctctgaat tttcagaaag cattcggttt
     4681 ccttaatctt ggtctaggca agttctactt tgagattata catttactag gaaaatgctc
     4741 cccccaccgt agtatggttt taaattttga ttttatgtgg cgtacaattc cgactatgca
     4801 tcttgaacaa ctgattactc attttggtta tcccgccctg ttgttgggac tcttgctgga
     4861 aggtgaaaca gtcctagtcc ttgcggcttt catggcgcat cgcggatatt tagacttgcc
     4921 gttggttatt ttgctcggtg gcttagttgc cttcgcttcc gaccaatttt tcttttggat
     4981 ggggcgtatt caaggaaata cgtttttggc aaatcgacct gcctggcagc cgcatgtaga
     5041 aaaagccaaa gctctacttg accggaactc cactctgctt tttctcagcg tgcgttttat
     5101 gtatggctta aggaccgtat taccttttgt gcttggtatg tccaaactga atacaaagaa
     5161 atttgtttta ttagatttta taggtgcttt tttgtgggca attacttttg ggttggctgg
     5221 gcattttatt gggcatgtga tgacattgat ctttgatgat gtgaaagagc atgaactgcc
     5281 cgtgatgatc gggatcgctt tgataggaat tggcatttgg ttgtatcgcc gtaacaataa
     5341 taaagataag gatggtaaat accatgaata atcattctca ggaacaattc aaaaacctgt
     5401 ttttagaaaa aatggaaaca tttaccagaa atgaatggga gctgcttcgc cacatcatgg
     5461 atcgcagaca cgtgacccgt aacgtcaaca aggagtttga tgaccaacta acctttggtc
     5521 agcgcctcgc ggataatgtt gccaaatttg gcggatcatg gacgttcatc attatctttg
     5581 gcttgatctt attgacctgg gtttttctca actcggttgt gcttgctagg ttgaatgatg
     5641 cttttgaccc atacccttat attttgctaa atctctttct ttccatgttg gcgtcgattc
     5701 aggctcctat tattatgatg tctcaaaacc ggcaggcaac gaaagaccgg atggatgcag
     5761 cacatgatta cgaagtgaac ttaaaagcag agatggagat cgaaacattg caccagaaat
     5821 tagatgaact gcgcgataag caatgggcag aactggtgga aatgcaacag gaacagattc
     5881 gattgttgac gaaactgttg gaagaaaaaa acaaataaac gtttgagtaa aacaggtcag
     5941 agcaactctg acctgttttg tttccatcgg gaaaactcgt gatagcccct tactggctgc
     6001 cagcggactg gcgctctgtc cttttggggt tgtttgcgtc ttggtttgga atgagatcag
     6061 ttggctaaat tagcatacaa ttgcacgggt tcattgctgt ttgtttctat taaagggcgg
     6121 taaaatttta aagcaaagaa aaagtcccgt tctactgaca ggaaacccat atgcgtattg
     6181 cgaaacattt tcggaaagat caagaaactc tccagcggtt tttagatgtg tttggcgcag
     6241 gatctgccat gcttggggga ggcaataaaa atgttcaacc gggttttttc atccatggca
     6301 gcaccttcat ccatgagtac gtggacggaa atttctttcg caaggaagaa ctgcttatga
     6361 aaactctcga agatgccggg tttcccgtca actcgggcgt gatcggaaat atgaaagatg
     6421 aacatgcgaa atgcaggcaa gccgcagacc tgctttccaa agccgccagt gactggcagg
     6481 gcggggatgc tggcgcccga attgaagtgg g
//
LOCUS       DAOQNJ010000077         6478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6670_length_6478_cov_6.588354, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000077 DAOQNJ010000000
VERSION     DAOQNJ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6670_length_6478_cov_6.588354"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..2477)
                     /locus_tag="PLG52_03625"
     CDS             complement(138..2477)
                     /locus_tag="PLG52_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB40564.1"
                     /translation="MNVQTQKILRDLWVNKSRSLLITLAVAIGVAAFGLMISGRIVLE
                     ENLRDVYAATKPAHAILSLSPFDQTLLTDVRAMDNVVAAQAYRADLARLKTGPDSWLG
                     FEIQTIPDFDNIAVNKITAEGELSNPPLNSIFLERSLKNVIDIGDTVTIRLLNGNEHT
                     LTVAGFVNDLSRLPAEISLAGIGYITPETANELELEPEYNQLLIRYADGSTRTEVEKL
                     TTTVTEAIEEDEYIVFNANVPVPDTYILGDNMTSVLFILNSLGFLTLILSAFLVTSVM
                     SAMMSQQIPQIGILKSLGAHVGQTMSIYFREVLLFGIFALILAIPMGLVGAYFLADGL
                     ASSMNFDVPNFYLPTQTLILQAISALLAPLLAALIPIISGSRITIREALSNYTPDSAS
                     NALSLGEIPQIANLSVRNTFRRKSRLALTFAALLLAGSMFISIIGIREEMRQASIEMQ
                     KEANYDLDVDLARPYSQSTLEETALRVKGVEEVETWAVDTGRLIFNKDRLSGSIILYG
                     IPQGTTMTQPAVINGTWLSKPKSIFVNNDFLLLSPDLKVGSVITLNIGGKEEEWTIVG
                     STGRGFIPIAYVYYDDLTKVTGLDGFASRLVVRTDKSDSAFQTSAQSDVLSHLAAEDI
                     DVVNSSTTTERNETSLEQMDTLIILLMAMVILIAIVGGLGLAITMSLNVMERTREIGI
                     LRSLGAQNGVVRRVVMIEGLVIGVISWVISIPCSVPLSIFLGNSLGLSLLARPLDYIF
                     SVPAMLIWLALTVVIAIIASILPAQSAARLTIRDALVYE"
     gene            complement(2474..3943)
                     /locus_tag="PLG52_03630"
     CDS             complement(2474..3943)
                     /locus_tag="PLG52_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB40565.1"
                     /translation="MTPNSASPLIEIKNLVKTYKTPAGDFTAVKGIDVEVQRGEFVAV
                     IGKSGSGKSTFINMITGIDRPTSGEIYIDGAPVHSFSEAQMAAWRGRNLGIVFQFFQL
                     FPTLTLLENVILPMELNHLYTKKERRERAMDLLKLVEMDHQAHKLPTTISGGQQQRVA
                     IARSLANDPPLLIADEPTGNLDSKTAAKIFSLFEELVASGKTILMVTHDSDLARRVNR
                     TILISDGEVVNEYLVRALATLTQDQLVEVARRVKPQIFPPDASITRQGEVGDKFYILL
                     DGKADVYLNEAGGGQVLVNELKRGQYFGEMALLGSGVRTATVKASPETSVSVAALDSM
                     AFNKLVKESRTLQDELKSIIERRNILDQLKVFASAMVKDPASKSPIEFGANQEIIKQG
                     DGGEVFYLILEGKVNVIVDDSQGEETIIEHLGKGAIFGESILLGDTRYRTTVRVSKEK
                     PAKVLAITPEEFNQFAEQNKQAEAKKKPTESSIRRKVRK"
     gene            complement(4020..4511)
                     /locus_tag="PLG52_03635"
     CDS             complement(4020..4511)
                     /locus_tag="PLG52_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNB40566.1"
                     /translation="MTKLDSMVQNFLAQKRIAVVGVSDKRETGCNAAYQKFKDAGYEV
                     FAVNPRIASFQGDACYPDLAALPTKPDAVFILANPKVTDQIVSQCVDLGVKHVWMHCM
                     MGTKPGLATSTTSVSPTAVDVCKANGISVIPGSCPNQYLNPDGGHKFMNGMFKLFGFM
                     SVN"
     gene            complement(4611..5516)
                     /locus_tag="PLG52_03640"
     CDS             complement(4611..5516)
                     /locus_tag="PLG52_03640"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB40567.1"
                     /translation="MDRKEFGILVSHLRQDLNLTQFELAENAEVDIAVISQIERGVKK
                     HLDSELLFKLANSFHLTTIERREFFLAACGLGETRIIRQPSAALATDTINAEKVLGKM
                     VKIVEKLSIPAFIMDVYSDVIAINYIAAAFFQVTPDVLDVASKVPGGYSTVRMMFGKD
                     LHLRSKVLENWDQYAVGSMRFVRENSLRYRAKPYFQYLMKSFQDRNEFPLFERYWRMV
                     SSFEQDREANVDVFSYTHADYGPLNYATATTVSITPHGELFLNQYLPLDDHTRNLFSA
                     LEQQTGQGVVTELASWPEKKIPMNT"
     gene            5781..>6478
                     /locus_tag="PLG52_03645"
     CDS             5781..>6478
                     /locus_tag="PLG52_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB40568.1"
                     /translation="MTDAQQFTFKAETKQLLQILIHSLYKDREVFLRELLSNASDAIN
                     RLRFEMLTNQNVLDADAELKIHVKVDKDNRTITVQDTGIGMTQDELVDHLGTIAQSSA
                     RKFLEAAKEKNQDASGLIGQFGVGFYSVFMVAEWVRVTSRSFRPDAQAVTWYATGDDT
                     YQVSPAEMTERGTRIEIKLKEDAAEFAEEYRLKNIIHKHSDYIGFPIYLGDEEKPVNK
                     QTSLWRTARNEITA"
BASE COUNT         1603 a         1449 c         1756 g         1670 t
ORIGIN      
        1 gtttcaattt caagaaaatc gaaaaatccc tgttcgaaga gttaagtcaa ctcaccaaac
       61 agggacatga acgcatgatg aaagaagaat tttcgtaatt gttttaggga cgatatgctt
      121 gttgctgtcc ctaaacttta ttcatacacc agggcgtctc gaattgtaag gcgggcggcg
      181 ctttgcgcag gcaggatgct ggcaatgatg gcgatcacca cggttagggc gagccagatc
      241 agcatggcag ggacggagaa gatgtaatcc aatgggcggg caagcagcga aagcccgagg
      301 gaattgccca aaaatataga gaggggaact gagcagggga tggagatcac ccaactgatg
      361 acaccgatca ccaagccttc gatcatcacg acccggcgaa cgactccatt ttgagcgccc
      421 agtgaacgca atataccgat ctcgcgggtg cgttccatca cattgagact catggtgatg
      481 gcaagcccca gtccgccgac gatggcgatc agaatcacca ttgccatcag aaggatgatc
      541 agggtatcca tctgttcgag tgaggtttca tttcgctcgg tggtggtgga tgaattgaca
      601 acatctatat cttcagcggc taggtgagag agaacgtcag attgagccga ggtttgaaag
      661 gctgaatcag atttatcggt ccgcactacg aggcggctgg cgaaaccatc cagccctgtg
      721 actttggtca aatcatcgta gtacacataa gcgatgggga taaatccgcg cccggtggaa
      781 ccaacgatgg tccattcttc ttctttgccg ccaatgttca gcgtaatgac agagccaact
      841 tttagatcgg gggagagcaa aagaaaatcg ttgttgacga agatgctttt gggtttggat
      901 aaccatgtgc cgttgatgac tgcgggttgg gtcatggttg tgccttgtgg aatgccatac
      961 aaaatgatgc tgccgctcag gcggtcttta ttgaaaatga gccgacctgt atccactgcc
     1021 caggtttcaa cttcttctac accttttaca cgcaaggcag tttcttccaa cgtgctttga
     1081 gaatatgggc gcgccaagtc tacatcgaga tcgtagttcg cttctttttg catttcaata
     1141 ctggcttgcc gcatttcttc gcgaatgccg ataatggaaa tgaacatgga accagccaga
     1201 agcagcgcgg cgaaggtgag tgcaaggcgg cttttgcggc ggaatgtatt ccgaactgaa
     1261 aggtttgcga tctgcggaat ttctccaagg cttaatgcat tggaagcact atcaggggtg
     1321 taattgctga gcgcttcacg gatcgtgatg cgcgaaccag aaatgatagg gatgagcgcc
     1381 gccaataatg gcgcaagcaa tgcactgatg gcttgcaaga tcagggtttg agtgggcagg
     1441 tagaaattcg gcacatcgaa gttcatgctc gaagccagac catctgcaag gaaataagcg
     1501 ccgaccaagc ccattggaat ggcgaggatc aaggcgaaga tgccgaacag cagcacttct
     1561 cgaaaataga tggacatggt ctgaccgaca tgcgcgccta aagattttag aataccgatc
     1621 tgagggatct gctgcgacat catggcagac atgacactgg tgacgaggaa cgcgctcagg
     1681 attagtgtga ggaagccaag cgaattcaaa atgaaaagaa ccgatgtcat gttatcgccg
     1741 aggatgtaag tatcgggcac aggcacattc gcgttgaaaa cgatgtactc atcctcttcg
     1801 atggcttcgg tcactgtggt tgtcagtttt tcaacttcgg tgcgcgtaga gccatctgca
     1861 tatcggatga gcagctgatt gtattccggc tcgagttcaa gttcgttggc tgtttctggg
     1921 gtgatgtaac caatccctgc caggctgatc tcagcaggta aacgagaaag gtcatttaca
     1981 aaaccggcaa cggtcagggt gtgttcattt ccgttcagca aacggatcgt gaccgtatcg
     2041 ccaatgtcga tcacattttt tagtgaacgt tccagaaaga tggagttcag cggcgggttg
     2101 gaaagttcac cctcggcggt aattttatta accgcgatat tgtcgaaatc aggaatagtt
     2161 tggatctcga agccaagcca ggagtctggt ccggttttga ggcgggcgag gtctgcacga
     2221 taggcttgtg ccgcaacgac attatccatg gcgcgcacat ccgtgagcag cgtttggtca
     2281 aatggcgaga gcgaaagaat ggcatgcgct ggtttggtcg cagcatacac atcgcgtaag
     2341 ttttcttcca acacaatgcg accgctgatc ataagcccaa aggctgccac cccaatggcg
     2401 accgccagcg tgataagcaa actgcgtgac ttgtttaccc acaggtcacg caggattttt
     2461 tgagtctgca cgttcatttg cggacctttc gcctgatgct gctttcagtg ggctttttct
     2521 tcgcttcagc ctgtttgttt tgttctgcaa attgattgaa ttcttctggc gtgatcgcaa
     2581 ggactttggc tggcttctct ttgctaacgc ggacagtggt tcgatagcgt gtgtcaccaa
     2641 gcaggatcga ttcgccaaag attgcacctt taccaagatg ttcgatgatg gtctcttctc
     2701 cttgagaatc gtccacgatc acatttactt tgccttcgag aatcagatag aagacttcac
     2761 cgccatctcc ttgcttgatg atctcctgat tggctccgaa ttcgatgggt gatttactcg
     2821 cagggtcttt gaccatggca gaggcaaaga cctttaattg gtcaagaata ttgcggcgtt
     2881 cgataatgct tttgagttcg tcttgcaggg tgcgtgactc tttcactagt ttattgaatg
     2941 ccattgaatc caatgcggcg acagagacgg atgtttctgg cgaggctttg acggtggcag
     3001 tccgtacgcc acttccgagt aatgccattt cgccgaagta ctgtccgcgt ttgagttcat
     3061 tcaccagaac ttgaccaccg cctgcttcgt tgaggtacac gtctgctttg ccatcgagca
     3121 agatgtagaa cttatcgcca acttcgcctt ggcgggtaat gctggcatca ggcggaaaga
     3181 tttgtggttt aacgcgcctt gccacttcca cgagttggtc ttgggtgaga gttgccagag
     3241 cacgcacaag atattcgttt acaacttcac catctgagat aagaatggtg cggttgacgc
     3301 ggcgagcaag gtcactatcg tgtgtgacca tcagaatggt tttaccggaa gcgacgagtt
     3361 cttcgaataa agaaaatatc ttggcggcag tttttgagtc gaggttgccg gtgggttcgt
     3421 ctgcaatgag cagtggtggg tcattggcaa gggagcgggc gattgccaca cgttgttgct
     3481 gcccgcctga aatagtggtg ggaagtttgt gagcctgatg gtccatttcg accagtttga
     3541 gcaggtccat ggcgcgttca cggcgttctt ttttggtata gagatggttc aattccattg
     3601 ggaggatgac attctctaac agggttaatg tggggaaaag ctgaaagaat tgaaagacga
     3661 tcccaagatt ccgtccgcgc caggctgcca tttgcgcctc actaaaagag tggactggtg
     3721 cgccatcgat atagatttcg cccgaggtgg ggcggtctat gccggtgatc atattaatga
     3781 aagtagattt gccgctgccc gatttgccaa tgacggcaac gaactctccc cgttgcactt
     3841 ccacgtcaat acctttgacg gcagtgaaat ctccagcggg ggtcttatag gttttcacta
     3901 agtttttgat ctcgatcaat ggggaggcgg aatttggagt catgttgtca agtttaatcg
     3961 aattaataaa aaaaacagcc atggttttgc catgactgtt caagaaagtt ggtgggcagt
     4021 cagttcacgc tcataaaacc aaatagcttg aacattccat tcatgaattt atgcccgcca
     4081 tcggggttga ggtattgatt ggggcaagag ccggggatga cggagatgcc attggctttg
     4141 catacatcta ctgcggtggg ggatacactg gttgtgctgg tggcgagacc gggcttggtg
     4201 cccatcatgc agtgcatcca cacatgtttc acgcccaagt ccacgcattg actgacgatc
     4261 tggtcggtca ctttggggtt ggcgaggatg aagaccgcat cgggcttggt gggcagagcg
     4321 gctaggtcgg ggtaacaggc gtcgccttgg aacgatgcga tgcgcgggtt gacagcgaag
     4381 acttcgtagc ctgcatcctt gaatttttgg taggcggcat tgcatccggt ttcgcgcttg
     4441 tctgaaacgc ctacaacggc gatccgtttt tgggcgagga agttctgaac catactatcg
     4501 agtttcgtca tgacattctc cttattcata ttgcttacgc ttcttgttac ataagcgtgg
     4561 cgctattaat gattataagg aggacattcc tgccttcaat atgacgaata tcaggtgttc
     4621 atagggattt tcttttcggg ccatgaggca agctctgtga caacaccttg ccctgtttgt
     4681 tgctcaagtg cagaaaaaag attacgggtg tggtcatcga gtggcagata ttggttgagg
     4741 aataattcgc catgtggggt gatcgagacg gtagtagcgg ttgcatagtt gagcggaccg
     4801 taatcagcat gggtgtaact gaagacatct acattggctt cacggtcttg ttcaaaggac
     4861 gagaccatgc gccaatagcg ttcgaagagc ggaaattcat ttcggtcttg gaatgacttc
     4921 atgagatatt gaaaataagg tttggctcgg tatcgcaagc tgttctcacg gacgaagcgc
     4981 atcgagccga cggcgtactg gtcccagttt tcaagcactt tggaacggag gtgcaggtct
     5041 tttccaaaca tcatgcggac ggtgctatac ccaccgggga ctttcgatgc gacatccaga
     5101 acgtcaggtg taacttgaaa gaaagcagcg gcgatatagt tgatggcaat cacatctgaa
     5161 tagacatcca tgataaatgc ggggatgctt agcttttcaa caattttgac cattttgccg
     5221 aggacttttt cggcattgat cgtatcggtt gcgagcgcgg cagagggttg gcggataatg
     5281 cgagtttcgc cgagcccgca agcagcaagg aagaactcac ggcgttcaat ggtagtaagg
     5341 tgaaacgaat tggcgagttt gaaaagcaat tctgaatcaa gatgtttttt cacgcctcgt
     5401 tcaatttgac tgatgactgc gatatccact tctgcatttt ctgccagttc aaactgcgtt
     5461 aagttgaggt cttgccgaag atgagaaacg aggattccaa actctttgcg gtccatgacg
     5521 gctcctacca aataaattcg actcaaaaat tttttcgagt caagtttaaa ctcttttgct
     5581 ggacttttca acccaaaatc gtacaatatt tgagttgttt gtttccgttc ttgcaaccct
     5641 gcctcatgta gtgggggagg ggaactacat ttgggacctc agccttaagg ctgaggtttt
     5701 attttaagta agtctcttac gaaattttta cgatttgcgg ctataaatag ggaaaatcct
     5761 tcatctttca ggaggcaacc atgacagatg cccaacaatt tacgttcaaa gcagagacca
     5821 aacaactgct gcaaatcctt atccattccc tgtataaaga ccgggaagtt tttttgcgtg
     5881 aactgctctc gaatgcgtct gatgcgatca accgcttgcg ctttgagatg ctgaccaacc
     5941 aaaatgtgtt ggatgcggat gccgaattga agatccacgt aaaagtggat aaagacaacc
     6001 gcaccatcac ggttcaggat actggcatcg gcatgaccca ggatgagctg gtggatcacc
     6061 tcgggacgat cgcccaatcg agcgcgcgca agtttttgga ggcggcgaag gaaaagaatc
     6121 aagacgcttc cggtttgatc ggtcagttcg gcgtgggttt ttactccgta tttatggtgg
     6181 cagagtgggt gcgggtcacg tcgcgttcct tccgccccga tgctcaagcc gtgacttggt
     6241 acgccacggg tgacgatact tatcaggtca gcccggcgga gatgaccgaa cgcggtacgc
     6301 gtatcgaaat taaactgaaa gaggatgccg ccgaatttgc cgaagaatac cgcctaaaga
     6361 atatcatcca caaacattcc gattacatcg gctttcccat ttatttgggc gacgaagaaa
     6421 agccggtcaa caagcagacc tctctgtggc gcacggctcg taatgagatc acggctga
//
LOCUS       DAOQNJ010000078         6424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6773_length_6424_cov_9.881300, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000078 DAOQNJ010000000
VERSION     DAOQNJ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6773_length_6424_cov_9.881300"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PLG52_03650"
     CDS             complement(<1..679)
                     /locus_tag="PLG52_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNB40569.1"
                     /translation="MQKQSLTSLWQFRQLGSIEWLPAQVPGGVHTDLMALARIPDPFV
                     GDNEKRVAWVAESDWEYHTTFNVQADVFQRKNIWLVCDGLDTLASLTLNGKALGETNN
                     MFRQYRFDIKPLLKAEGNELTITFKSPVKFVAEKNTARPMNGVSQAIAGGPHLRKAPC
                     HFGWDWGPQLPPIGIWKDIYLEAYDTARISEVHLRQKHDNGKVTISARVEVENGNAPL
                     SATLLVTA"
     gene            complement(730..1542)
                     /locus_tag="PLG52_03655"
     CDS             complement(730..1542)
                     /locus_tag="PLG52_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40570.1"
                     /translation="MPSEKKLYLSAKGITKTFGFGRQKTVAVDNVDLDLYEGEVVSIV
                     GESGSGKTTLSKVLLGLMNQTSGDVYFQGVKRDISTQSRKQEYWKNIQAIFQDPFSSY
                     NIFNKIDSVLLDCINMRGGKYLPREKKLEMMTEACSFVNLKFEELTNKYPFELSGGQM
                     QRLMIARIFLLQPKILLADEPTSMIDACSRATILDELMHLRKEIGMTIIFITHDIGLA
                     YYVSDSVYIMEKGKFVEGGSADEVILTPRHPYTKRLINDVPKIYEEWDLSTV"
     gene            complement(1532..2563)
                     /locus_tag="PLG52_03660"
     CDS             complement(1532..2563)
                     /locus_tag="PLG52_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40571.1"
                     /translation="MTNTMLDVRELSTKYVTRFNESVYAVDNVSLKVEEGRSLGIAGE
                     SGCGKSTLALSLMGYYFPPLHYMNGDIIINGKNISGLKPDDVRKSILGKEIAYIPQAA
                     MNALNPTRKIINFIEDVILEHEMYVTKKDIYERAKKLFETLGLPPEVLHKYPVELSGG
                     MKQRTVIAISVLLLPKVLIADEPSSALDVTSQKMVIKMLKQLMDAGFISSMIFITHEL
                     PLLYNVVDDIMVMYAGQIVEKGTSHEMVFDPIHPYSKGLMGSIIVPEKGLRDVKLTAI
                     PGVPPNLKKPPSGCRFAERCRFVRPECRVASVPLHEASNGRMYRCIIPEAELRAIYAR
                     EQEEQKNAK"
     gene            complement(2573..3823)
                     /locus_tag="PLG52_03665"
     CDS             complement(2573..3823)
                     /locus_tag="PLG52_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40572.1"
                     /translation="MKHIIQQVFRSGKFVTGFSIFSAIILIVIFYPLIITDQPLAIIG
                     QGTFFPPGIYVSVYDSMSANAYTLNLDEAAEMRIASKLNDEDRLAVKEWLVAAGIPES
                     DIDTTDTAKLLDQWFANYDSKKNVPGMTNAKRNYYIRLNNALKGLLSAEAATVMQTSP
                     QTNELEQIDTIQQTDYVNVGEVANVRVLPLGTDNFGRDVLTELVSATRVSLQIGLVAG
                     IIATIIGLTLGLLAGYIGGLVDDLIMFVTNLFIVIPPFVLLILISFSIGQEQRGAITV
                     AVVIGLTSWVWTARAVRSQVISLRNRDHVNLSKLSGHSIVHIILNDILPYIMSYVIMA
                     LILQISSGILAEAGLSILGLGPRTTEVPTLGLMMNWAMIYQAHILGNWWAYMPVLVTI
                     ALITFSMNLMNTGMDQVFNPALRD"
     gene            complement(3838..4845)
                     /locus_tag="PLG52_03670"
     CDS             complement(3838..4845)
                     /locus_tag="PLG52_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40573.1"
                     /translation="MLKGYLKYFLNKLGWFAITFLFAFILNFLLPRLMPGDPVAAIVS
                     RLAQGMSNTSGVQAIYEQYTELFGTNKPMIEQFLIYVKNVARGDFGFSFSQYPRTVSD
                     VIGASIWWTLALQFPAIIVGWIIGNTLGALAAYLKGGFDKILMPISIFVSNFPAFGMA
                     VIFMVIFGVNLKWFPTSGGYGFDMIPNFSWAFIKSVIIHYQLPFWSIVVIAIGGQAIG
                     MRSMSIYELNADYVKYARFMGIRDSKIVGYVFRNAMLPQVTGLALSVGTMVGGALVAE
                     IVFSYPGLGTTLLRAVLGQDYPLISASTLIITVMALFANFIIEILYGLIDPRIKAAQS
                     D"
     gene            complement(5115..>6424)
                     /locus_tag="PLG52_03675"
     CDS             complement(5115..>6424)
                     /locus_tag="PLG52_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB40574.1"
                     /translation="SGKAVNPLIVSAYLSSNYVIQKAWTQTLESRSADGTALMADAAD
                     DVVWSGPYTKYFADDTKVVMIRDDNYWGQDASMWGALPAPKYLAHVIYKDNAAGTTAL
                     QAGEVDVSQQFNSNVQDLWLKDGLAVSTYLPEAPYQIGASLPTAFFNLNSNGLDQVAV
                     RKAIAMAVDYPTIIANAMTNQSATFDQAPRSLMNPTAGEQAMYDSAAVADLQWAGNDI
                     EGAKALLDEAGIVDSNGDGNREYNGQELHYVATCPNGWSDWQAAIEVVAAAGKEIGID
                     ITTNYPEWSVYQTVVTKSDTALPEGYDIFMMWSDGAGPTQPWSRIRHLISSEFVGDAV
                     NWNGNWGQYSNPAADELINAIPNESDPAKLNEMYTELVKIYLTDVPSFTLMYRPQAFH
                     TVNESVWTNFPHGEDGTNPPVPPLDLTDGWSIAGLYNLTLVNP"
BASE COUNT         1775 a         1497 c         1495 g         1657 t
ORIGIN      
        1 gagcagtaac aagtaaggtc gcagaaagag gcgcgtttcc gttttccact tccacccgcg
       61 cagaaattgt caccttgcca ttgtcatgtt tttggcgaag atgaacttca gaaatacgag
      121 ccgtgtcata agcttcgaga taaatatctt tccagatgcc gatcggcgga agttgtggac
      181 cccaatccca gccgaaatga catggagctt tgcgcaggtg tggtccaccg gcgatggctt
      241 gcgaaacgcc gttcatgggg cgcgctgtat ttttctcggc gacaaacttg acgggtgatt
      301 tgaacgtaat ggtcagttcg ttgccttcag ctttgagcaa gggctttata tcaaaacgat
      361 attggcggaa catgttattg gtttcgccaa gcgcttttcc attgagtgtg agtgacgcaa
      421 gcgtatccaa cccatcacaa acaagccaga tatttttgcg ctgaaagaca tctgcctgca
      481 cattgaaggt ggtgtgatat tcccagtcgc tttcggcaac ccaggcaact cgtttttcat
      541 tatcgcctac aaatgggtct gggatgcgtg caagcgccat gagatccgtg tgaactccgc
      601 ccggaacctg ggctgggagc cattctatag aacccaactg gcgaaactgc cataggcttg
      661 ttagagattg tttttgcatt ggcattcttc ctgaatgttt tcttctttag atttttattg
      721 ctcgtgcttt tatactgtgg agagatccca ttcctcgtag atcttcggca catcgttaat
      781 gaggcgtttg gtataaggat gccgcggagt gagaatcact tcatctgccg agccgccttc
      841 cacaaatttg cctttttcca taatatagac actatctgaa acatagtacg ccagaccaat
      901 atcatgcgtg atgaagatga tggtcatgcc aatctctttg cgaagatgca tcaattcatc
      961 cagaatagtt gcgcgagagc aggcatcgat catggaagtt ggctcatcag cgaggagaat
     1021 ttttggctgc aacaaaaaga tgcgggcgat catgaggcgt tgcatttgtc cgccggaaag
     1081 ttcgaaggga tatttattgg tcaactcttc gaacttgagg ttcacgaagc tgcatgcctc
     1141 agtcatcatt tctaacttct tttcacgtgg caaatatttt ccgccgcgca tgttgatgca
     1201 gtccagcaag accgagtcga tcttattgaa gatgttatag gaagagaacg ggtcttggaa
     1261 gatggcttga atattcttcc aatattcctg ttttctggac tgggtgctaa tgtcgcgctt
     1321 gacgccttgg aagtacacat cgcccgatgt ttggttcatc aatcccaata gcactttaga
     1381 gagggtggtc ttgccgctgc ccgactcacc cacaatggaa acaacttcac cttcatacaa
     1441 gtcgaggtct acattatcca ccgccactgt tttttggcgt ccgaacccga atgttttggt
     1501 gatgcctttt gcgctcaggt agagtttctt ttcacttggc atttttctgt tcctcctgct
     1561 cacgagcata gattgcgcgc agttcagctt ctgggatgat acagcgatac atacgtccat
     1621 tgctcgcttc atgtaaaggc acacttgcga cacggcattc cggtcgaaca aagcgacagc
     1681 gttcggcgaa gcgacaacct gaaggcggct ttttgaggtt aggtggcacg ccaggaatgg
     1741 cggtcagttt gacatcgcgc aaaccttttt caggaacgat gatcgaaccc atcaatcctt
     1801 ttgaataagg atgaatggga tcgaagacca tttcatgcga agtccccttc tccacgatct
     1861 gaccggcgta catgaccatg atgtcatcca ctacgttata aagcagaggc aactcatgcg
     1921 taatgaaaat catggagcta atgaaaccag catccattaa ctgcttaagc atcttaatga
     1981 ccattttttg cgaggtcaca tcgagtgctg aagagggttc atctgcaatc aacactttgg
     2041 gtagaagcag tactgaaata gcgatcacag tacgctgttt cataccgcct gaaagttcaa
     2101 ctggatattt atgtaaaact tcgggaggaa gacccaatgt ttcaaagagt tttttcgcgc
     2161 gttcgtaaat gtccttctta gtaacataca tctcatgttc aagaatgaca tcttcaataa
     2221 agttaatgat cttgcgtgtt gggtttagag cgttcattgc cgcttggggg atataggcaa
     2281 tctctttacc aagaatggat ttgcgcacat cgtctggctt caaaccggag atattcttcc
     2341 cattaataat gatgtcaccg ttcatgtagt gcaaaggcgg aaagtaatat cccatcaggc
     2401 tgagggcgag ggttgatttt ccacagccag attcccctgc aatcccaagc gaacgtcctt
     2461 cttctacttt aagtgacaca ttatcaaccg catatacact ctcattaaag cgggtaacat
     2521 attttgtaga tagttctcta acatctaaca ttgtgttcgt catattaatc tcctagtctc
     2581 gcagggctgg gttgaagacc tgatccatac cggtattcat caggttcatc gagaaggtaa
     2641 tcagcgcgat ggtaacgagc accggcatat aagcccacca attgccgaga atatgtgcct
     2701 gataaatcat cgcccagttc atcatcaatc ccagagtggg gacttccgta gtgcgcggtc
     2761 ccaacccaag gatagaaagc cccgcctctg ccaaaatacc agaggagatt tgcaaaatga
     2821 gcgccatgat cacatatgac atgatgtatg gcaagatatc attgagaatg atgtggacaa
     2881 tggagtgtcc agagagtttt gaaagattga catggtcgcg gtttcgcaaa gaaatgacct
     2941 gcgaacggac agcacgtgct gtccacaccc atgaggtaag cccgatcact actgcaacag
     3001 taattgcgcc acgttgctct tgcccaatac taaatgagat gaggatcaat aacacgaacg
     3061 gcgggatgac aataaaaagg ttcgtgacaa acatgatgag gtcatctacc aacccgccga
     3121 tatagcccgc gagcaaaccc aaggtcagac cgataatggt ggcaattatt cctgccacaa
     3181 gaccaatctg caaagagaca cgggttgcag agaccagctc agttagcaca tctctgccaa
     3241 agttatctgt accaagcggt agcactctca cattggcaac ttcgccgaca ttcacataat
     3301 ctgtctgttg aatagtatca atctgctcta gttcattggt ctgcggactg gtctgcatca
     3361 ccgtcgccgc ttcagcagaa agcaaccctt taagggcgtt gttgaggcga atgtagtaat
     3421 tacgtttagc attcgtcatg ccaggcacat tctttttcga gtcgtaattt gcgaaccact
     3481 gatccagaag ttttgctgta tctgtagtat caatgtcgct ttctggaatg ccagcagcaa
     3541 ccaaccattc ttttacagcc agacgatctt catcatttaa cttactagca atacgcattt
     3601 cagctgcttc atctaagttg agtgtgtatg cgttcgcgct catactatcg tatacactta
     3661 cgtaaattcc tggaggaaag aacgtacctt gaccaataat tgccaaaggc tgatcggtaa
     3721 tgatcaatgg ataaaagatc acaatcagaa tgatggctga aaaaatagag aagcctgtaa
     3781 cgaacttacc cgagcgaaaa acctgttgga ttatatgttt catgctggtt tctccactta
     3841 gtcagattgt gcagctttga tgcgcggatc gatcaagcca tacaagatct caataatgaa
     3901 attggcaaat aatgccataa cggtgatgat caaggttgat gcagagatca atggatagtc
     3961 ctgtcctagc acagctcgca acagggttgt gccaagcccg gggtaactga aaacaatttc
     4021 agcaacaagt gcaccgccaa ccattgttcc aaccgacaat gccaaaccgg tcacctgcgg
     4081 aagcatagca tttcggaaca catagccaac gatcttgcta tcacgaatac ccataaagcg
     4141 ggcatacttc acatagtctg cattcaattc ataaatagac atagaccgca taccaattgc
     4201 ctgtccacca atggcaatca ctacaattga ccagaatggc aactgatagt gaataatgac
     4261 cgatttaata aatgcccagc tgaaatttgg aatcatatcg aaaccatatc cacctgaggt
     4321 agggaaccat ttgaggttga ccccaaaaat aaccatgaaa ataaccgcca taccgaatgc
     4381 cggaaaatta ctgacgaaaa tgctaatcgg cataaggatc ttatcaaaac cgcccttaag
     4441 ataagctgct aaagcgccca aagtatttcc gataatccat cccacaataa tggcagggaa
     4501 ctgcaaagca agtgtccacc aaatggaagc accaatgaca tcagatactg ttctaggata
     4561 ttggctgaaa gaaaaaccga agtcgccacg agcgacattc tttacataaa tcaaaaattg
     4621 ttcgatcatc ggtttattcg tgccgaacaa ttctgtgtac tgctcataaa ttgcctgaac
     4681 accagaggtg ttactcattc cctgcgccaa acgcgaaaca atcgcagcaa cagggtcacc
     4741 gggcataagg cgcggaagaa ggaaatttag aataaacgca aagagaaacg taatggcaaa
     4801 ccaccccaat ttatttagaa aatacttcaa atatcctttc aacacacacc tccatgtatt
     4861 cagcgggttg cttttatcta ctatcgctta ttttaagaga tttcacttaa attgctatct
     4921 tcagttttct tggtctctcg tcaaaaaacc tcccctactc tcttctaaaa aagagaggaa
     4981 atgttcttag aagaatttct taagcagagc aagggatgat ttttgaaggt agacgctttt
     5041 atcatcatga tggttacaag aacagccatg ccccatacag gatggaacat ggctgttttt
     5101 gaacacaaac ttgattacgg gttgacgagc gtcaggttat agagacctgc gatgctccag
     5161 ccgtctgtta gatcgagcgg gggcacaggc gggttggtgc catcttcacc atgcgggaag
     5221 ttggtccata cgctttcgtt cacagtgtgg aacgcctgcg ggcggtacat gagtgtgaag
     5281 gaaggtacat cggtaaggta gatcttgacc aattcggtgt acatttcgtt caacttggca
     5341 ggatcgctct cattcggaat tgcgttgatc aattcgtcag ctgccgggtt ggagtattga
     5401 ccccagttac cgttccagtt gaccgcatca ccaacgaact cagagctgat gaggtggcgg
     5461 atacgagacc aaggctgtgt aggaccagca ccatcagacc acatcatgaa gatgtcgtag
     5521 ccttcgggga gtgcggtgtc agacttggtt acaacggttt gatagacaga ccattcagga
     5581 tagttggtgg tgatgtcgat gccgatttct ttaccagcag cagccacaac ttcaattgca
     5641 gcttgccagt cagaccaacc attcgggcag gtagctacat agtgaagttc ctgtccattg
     5701 tattcgcggt taccatcgcc attggagtca acgataccag cttcatcgag caacgctttt
     5761 gcgccttcga tgtcattgcc agcccactgc aagtcggcaa cagcggcaga gtcgtacata
     5821 gcctgttcac cagctgtcgg gttcatgaga gagcgcggag cctgatcgaa agtagcagat
     5881 tgattggtca tcgcgttagc aatgatggtt gggtaatcaa ctgccatagc aattgctttg
     5941 cgaactgcaa cctggtccaa accattggag ttgaggttga agaaagcggt tgggaggctt
     6001 gcgccaatct gataaggagc ctcaggtaaa taggtggaga ctgctaaacc gtctttcaac
     6061 cagagatcct gaacattgga gttgaattgc tgtgatacat ccacttcacc agcctgaaga
     6121 gcggtggtgc cagcggcatt gtctttgtag atcacatgcg caaggtactt aggagcagga
     6181 agagcgcccc acatggaagc gtcttgaccc cagtagttat catcacggat cataacgact
     6241 ttggtgtcat cagcgaagta cttggtataa ggaccagacc aaaccacgtc atcggcggcg
     6301 tcagccataa gggcagtgcc atcagcagag cgggattcga gggtttgggt ccaagccttc
     6361 tggataacgt agttgctgct caagtaagca gaaacgatca gagggttaac agccttgcca
     6421 ctgt
//
LOCUS       DAOQNJ010000079         6418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6783_length_6418_cov_10.199749, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000079 DAOQNJ010000000
VERSION     DAOQNJ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6783_length_6418_cov_10.199749"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PLG52_03680"
     CDS             complement(<1..1235)
                     /locus_tag="PLG52_03680"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNB40575.1"
                     /translation="MQARKLTRRDFLKFGGALAATSALAACAPGEENAITQPFEGTTT
                     LQNPVITPEAQTSLPPVSMAILALSRLTFGIRPGDVDAFNALGATDDERLSAFVTQQL
                     NPDSINDSEFEARYANAGFETLHKSHEQLYADHIASNPYDSNDDAYWQWYSKPTYELV
                     DATILRAVYSKKQVVELLADFWHNHFNVYFWQDDGVPLLVSYNRDVLRAHMLGNFRQM
                     LEAVARHPSMLYYLNQNNSSDAGPNENFARELFELHTLGAENYLGVRDPNTVEKDANG
                     IAVGYVDNDVYESARCLTGWRVDDDLGDWEDGVEKTGNFLYYKPWHDRFNKLVLGKYI
                     PADQEDMKDAHDVLDLLAYHPGTARHIARKLCTRFISDVPSESIVQAAANTFIQFKDA
                     PDQLKRVMETILLSAEFKA"
     gene            complement(1334..2065)
                     /gene="lipB"
                     /locus_tag="PLG52_03685"
     CDS             complement(1334..2065)
                     /gene="lipB"
                     /locus_tag="PLG52_03685"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNB40576.1"
                     /translation="MPLHIIGMNIEILDLGLIDYEQAWKLQDEYAGEIAKGLRPPTLL
                     LLEHPHVYTFGRKGNASNLLWNENQLKEKGIATYWVDRGGDVTYHGPGQLVGYPLIPL
                     GRLNQDNRLPEADYVGYIRKLETMLIGVLMNYGIASGQRPGKTGVWIQADVHSRCIHC
                     KPEDKQKPAKIAAIGVKVDVNGVTRHGFALNVNPDMDYWNGIIPCGLTEPVVSLADLL
                     NQAPSMQEVKQHIKEAFCTVFENSM"
     gene            complement(2089..3483)
                     /locus_tag="PLG52_03690"
     CDS             complement(2089..3483)
                     /locus_tag="PLG52_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB40577.1"
                     /translation="MRTTFTKLENFGHSLEAPAYVLKPRNSEEIYSAFKQAKKMGLTV
                     TARGAGRSYNDASLNGGGIVLNMTGMDQILEWDPATGLVRCEPGVTLEKLWQQVEPDG
                     WWPPVVSGTMRTTLGGCLSANIHGKNNFKMGTIGEHVVEFTATLPTGAEVTCSPKKNA
                     DLFYSMISGYGMLGIFTSVTMQMKRLHSGLLVVDALPVHNLREHLSALQDGAPHNDYI
                     VGWLETFASGKALGRGQIHAARYLHEGEDPNPQETMKLKNQILPPMIFGVFPKSILYQ
                     FMKPFANNLGWRLVNIAKYVASLPKHTFRQSHAAFHFLLDYVPNWELSYGRGGLIQYQ
                     SFLPKETAESAWREVLSLSQKRGMPSYLGVTKRHRPDKFLLTHAVDGFSLALDFKVSD
                     STRPKMAAMLQEFDKIVLAHGGRFYFAKNGETTAETAQAFYGEETVKKFKKLKKRCDP
                     DGLLESDLYRRIFG"
     gene            complement(3492..4274)
                     /locus_tag="PLG52_03695"
     CDS             complement(3492..4274)
                     /locus_tag="PLG52_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB40578.1"
                     /translation="MSDLKPATPLNPRRRAIVIGASVGMGAALSKKLAKEGYVVALVA
                     RQKEKLNELCAEINESTEEVRAFAYPHDVSNYKEAPELLKNIVAEIGGLDTFIFMAGV
                     NYPPGGNDIYNFENDRQMVEINLIGAMAWLSPVAEMFQAAKYGQIVGVGSVAGDRGRV
                     GNPGYNTSKAGLATYLEALRNRLTRHGVNVLTVKPGFVKTEMLKAAVGPTPFAIPVEK
                     AVDDIYKALQKRKQSIYTPFFWQYIMLIIRHIPSFIFRRLSF"
     gene            4423..5685
                     /locus_tag="PLG52_03700"
     CDS             4423..5685
                     /locus_tag="PLG52_03700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNB40579.1"
                     /translation="MNSNHGKHGSVFTRAWDIISRDDTIPPEEKKAILRLIQVIVEAR
                     EQASDKRVGDDEEKSITHEVISKHSLMMTVKHQADELDALRRISLNLTSNLDLQKVLD
                     ILVTESMGLIKNANNVTIFLYERGELEFGAALDDKGERNIPVSKPRKNGLTYSVATSG
                     QQIIVEDMKSHPLYQNTSLNLTGSIIGVPLKFKDEVVGVMNLSKKVVGKFTRSELRLI
                     NLLADQAAVAIFNASMYKRLAQMANTDSVTGLPNRRALDERLQEEWRLAEQTKFPFAV
                     VMMDLDGFKAVNDTFGHNIGDDLLYSLFNFLAQRMRSSDFLARYGGDELTLVMRNTDA
                     EAAQSVMKKILELMQDYHFPFPKGKDMKLGITAGIAIYPTHANTPSDLIRAADSALYH
                     AKKYARGEYVVAKAITGRLNPVQVKDGS"
     gene            5757..>6418
                     /locus_tag="PLG52_03705"
     CDS             5757..>6418
                     /locus_tag="PLG52_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNB40580.1"
                     /translation="MPRKVKLILNPMADMGRAWKTANALRPIAQEFQGELTWSGTVYP
                     THAIELARQAAEEGYDMVIAMGGDGTVHEVMSGLMQVPAEKRPVMGVVPIGSGNDFAY
                     SIGIHQKPDHALAHALKAENIKPVDIGLMTDEHGRKEYFDNTLGIGFDAVVTIRSHKL
                     PIVKGFLMYLTAVIQTILLNHNPAKLNIETDHQSWSASALMLTLCNGPREGGGFMLSP
                     KS"
BASE COUNT         1651 a         1432 c         1621 g         1714 t
ORIGIN      
        1 ctggctttaa actccgctga aagcaggatg gtctccatta cacgcttgag ttggtctggt
       61 gcatctttga attgaataaa agtattggct gcagcctgaa caatcgactc ggatggcaca
      121 tcggaaatga agcgtgtgca aagttttctt gcaatgtggc gagccgtgcc cgggtgatag
      181 gctaaaaggt cgagtacatc atgagcgtct ttcatatctt cctgatcggc aggaatgtat
      241 ttgccgagca cgagtttgtt gaaacggtca tgccagggtt tgtaatacag gaagttgcct
      301 gtcttttcaa caccatcttc ccaatcaccg agatcatcat ccacccgcca gccggtgaga
      361 cagcgcgcag actcgtagac gtcgttatca acatagccga cggcaatgcc attcgcatct
      421 ttctcaaccg tgtttgggtc gcgcacgccc agataattct ctgcgccgag tgtgtgaagc
      481 tcaaataatt cgcgtgcgaa attctcgttg ggacctgcgt cgctgctgtt gttttgattc
      541 aagtagtaca acatagaagg gtgccttgcg acggcttcca acatttggcg gaagttgcca
      601 agcatatggg cacgcaacac atcgcggttg tatgaaacca gcagtggcac gccatcatcc
      661 tgccaaaaat agacattgaa atggttatgc cagaaatctg cgagaagttc cacaacttgt
      721 tttttgctgt atacggctcg taagatggtg gcatctacta attcataagt gggtttgcta
      781 taccattgcc agtatgcatc gtcattagag tcgtatggat tgctggcgat atggtctgcg
      841 tataactgtt catgcgattt atgcaaggtc tcaaacccgg cattggcata acgtgcttca
      901 aactcactgt cattgatgct atctgggttg agttgttgag ttacaaaagc tgaaagacgt
      961 tcgtcatctg ttgcgccaag ggcattgaaa gcatccacgt caccggggcg aatgccaaat
     1021 gtaaggcgac tcaaagctag gatcgccatc gaaacagggg gaagagacgt ttgtgcttcg
     1081 ggtgtaataa ccgggttttg tagagttgtt gtcccttcaa aaggctgcgt gatcgcgttt
     1141 tcttctcccg gcgcacaagc tgctaaagca gatgttgctg ccaaggctcc gccaaacttt
     1201 agaaaatcac gtcgcgttaa ttttctagct tgcataagac ctccaacaaa aaatggattt
     1261 caggcttttt tacttataca gttcggttct tattataaat atcccgctta agtaccgaat
     1321 aagttttcgt ggtttacatg gagttttcaa aaactgtaca aaaggcttct tttatatgct
     1381 gttttacttc ttgcattgac ggcgcctggt tgagcaggtc tgccagtgaa acgacgggtt
     1441 cggttagccc gcaagggatg atgccattcc aataatccat gtcagggttt acgttcaagg
     1501 cgaacccgtg ccgggtgact ccgttcacat ccactttcac accaatggcg gcgatcttgg
     1561 cgggtttctg cttatcttct ggcttgcaat ggatgcaacg cgaatggaca tcggcttgaa
     1621 tccatacgcc agttttgccg gggcgttgac cagaggcaat gccatagttc atcaaaacac
     1681 caatcaacat ggtttcgagc ttgcgaatat acccaacgta atcggcttcg ggtaaacgat
     1741 tgtcttggtt caatctccct agtggaatta acggatagcc caccagttga ccaggtccat
     1801 gataggttac atcaccacca cggtctaccc agtaagtggc gatgcctttt tctttgagct
     1861 gattttcatt ccaaagtaag ttgctggcat tgcctttacg cccaaaagtg tacacatgtg
     1921 gatgctcaag caataaaaga gttggcgggc gcagcccttt cgcaatttcc cccgcgtatt
     1981 catcctgaag cttccaagct tgttcgtagt cgattaaacc taaatctaaa atttcgatgt
     2041 tcataccaat aatatgtagg ggcatgattt atcatgcccc tgttcaaatt atccaaatat
     2101 tcttcgatac aaatctgatt ccaataaacc gtctgggtcg cagcgtttct ttaatttttt
     2161 gaacttcttg acagtctctt cgccgtaaaa agcctgcgct gtttcagcgg ttgtttcacc
     2221 attcttggca aagtaaaaac gcccaccatg agctaggacg atcttatcaa attcctgtaa
     2281 catggctgcc atcttcggac gagtggaatc ggacactttg aaatccaacg cgagcgagaa
     2341 accatctaca gcgtgggtga gcaaaaattt atcggggcgg tggcgcttgg tcacaccgag
     2401 gtacgagggc ataccgcgct tttgtgaaag gctcaacact tctcgccatg cagattcggc
     2461 tgtctctttg gggaggaaag attgatattg gatcaagccg ccgcgtccat atgaaagctc
     2521 ccaattcggc acataatcta acaagaaatg aaatgccgca tgtgattggc ggaaggtatg
     2581 ttttggaagg gatgccacat actttgcgat attcaccaaa cgccaaccga gattgttcgc
     2641 gaacggtttc atgaactgat ataagatgga tttcggaaag acgccaaaga tcatgggcgg
     2701 aagaatctgg ttcttcaact tcatcgtctc ttgcgggttc gggtcttcgc cttcgtgcaa
     2761 atagcgtgcg gcatgaattt gtccgcgccc taatgcttta ccgcttgcaa aggtttccag
     2821 ccagccaaca atgtaatcgt tatgcggggc tccgtcctgc agagcagaaa ggtgttcgcg
     2881 gaggttatgt acggggagcg catccacaac taaaagtccg gagtggaggc gtttcatttg
     2941 catggttacg gaagtgaaga tgccaagcat cccatatccg ctgatcatgg agtagaacaa
     3001 gtccgcattc tttttgggcg aacaagtcac ttccgcgccg gtgggtagtg tggctgtaaa
     3061 ctccaccaca tgttcaccga tggttcccat cttaaaatta tttttgccgt ggatgtttgc
     3121 actgagacaa ccgcccaatg ttgtgcgcat cgtaccagag acgacgggcg gccaccagcc
     3181 atctggttcg acttgttgcc aaagtttttc aagcgtgacg ccgggctcgc atcgcaccaa
     3241 gccggtagct ggatcccatt ccagaatttg atccatgccg gtcatgttga gcacaattcc
     3301 gccaccgttg agggaagcat cgttataact tcgcccggct ccacgagctg tgacggtaag
     3361 ccccattttc tttgcttgct tgaatgcaga atagatttct tcgctgttgc gaggttttag
     3421 aacatacgcc ggggcttcaa gagaatgacc aaaattttcg agcttcgtaa aagtagtacg
     3481 catgaggttc gttagaaaga gagacgtctg aatatgaaag aggggatatg ccgaatgata
     3541 agcatgatgt attgccagaa gaaaggcgta tagatggatt gctttcgttt ctgcagagct
     3601 ttgtaaatgt catccactgc tttttcgacc gggattgcga acggagtggg acctactgcg
     3661 gctttgagca tttcggtttt cacgaagcct ggtttgactg tgagcacatt caccccatga
     3721 cgggtgaggc ggtttcgtaa ggcttccaga taagttgcca acccagcttt ggatgtgttg
     3781 tagccggggt tgcctactcg tccacggtca ccagcaacgg agccgacacc aacaatttgc
     3841 ccatattttg cggcttggaa catttccgcg actgggctga gccatgccat agccccaatc
     3901 aggttgattt ccaccatttg gcggtcgttc tcgaaattgt agatgtcgtt cccaccgggc
     3961 gggtaattga caccagccat gaaaataaaa gtatctaaac cgccaatttc agcgactata
     4021 ttctttaaga gttctggagc ttctttatag tttgaaacat cgtgcgggta agcgaaggca
     4081 cgtacttcct cagtagattc gttgatttcg gcgcagagtt cattcagttt ttctttttga
     4141 cgggcaacta aagcaacgac atacccctct ttggcaagtt ttttagagag cgcagctccc
     4201 attccaacag aagcgccaat cacaatggct ctgcggcgtg gatttaatgg tgttgcaggt
     4261 tttaagtcac tcacaatgac ctcgtattaa gtatttattc atgtcattct aacacagcaa
     4321 taaatgacct ttcgggtcga aatagaatat gtatatgaaa gtgtataata gtacaaagga
     4381 tgtctcgaca gttaatgctt tttaggcata attctaaaaa acatgaatag caatcatggc
     4441 aagcacgggt cggtctttac gcgagcgtgg gatattatct ctcgtgatga tacgattccg
     4501 ccagaagaga aaaaagccat ccttcggctc attcaggtta ttgttgaggc acgtgagcag
     4561 gcttctgata aaagagtagg ggatgatgaa gagaaatcca ttacccatga agtgatctcc
     4621 aaacactcgc tgatgatgac cgtcaagcat caggcagatg aactggatgc gttgcggcgc
     4681 atcagcctta atttgacctc aaacctagac ctgcaaaagg tgttggacat cctcgtgaca
     4741 gagtcgatgg ggttgatcaa gaatgcaaac aatgtgacca tcttcttata tgaacgcggc
     4801 gagctggaat ttggggctgc attggatgac aaaggggaaa gaaatatccc ggtttcaaaa
     4861 ccacgaaaga atgggttgac gtattctgtc gccacctcgg gacagcaaat tattgttgag
     4921 gatatgaagt ctcatccgct ttaccagaac acctcattga atttaacagg gtcaattatt
     4981 ggtgtgccgc ttaaatttaa agatgaggtt gtgggggtga tgaacctttc gaaaaaggta
     5041 gtgggaaaat ttacccgctc tgaattgcgt cttattaact tattggcaga tcaggctgcg
     5101 gtagccatct tcaacgctag catgtataaa cgcttggcac aaatggcgaa tacggatagt
     5161 gtgacagggc taccgaaccg ccgtgccttg gatgaacgct tacaagaaga atggcggctt
     5221 gccgaacaaa ccaagtttcc ctttgcggtt gttatgatgg acttggatgg tttcaaagcg
     5281 gtaaatgaca cctttggtca taatattggg gatgatctgt tgtattcctt gttcaacttt
     5341 ctggcacaac gaatgcgcag ttcagatttc cttgcgcgtt atggcgggga cgagttaact
     5401 ttggtcatgc ggaatacaga tgccgaagcg gctcagtctg tgatgaaaaa gattctcgaa
     5461 ctcatgcagg attatcactt cccatttcca aaagggaagg atatgaaatt gggcattaca
     5521 gcagggattg cgatttaccc gacccatgcc aataccccaa gtgacttgat ccgtgcggca
     5581 gattcggctt tgtatcatgc caagaaatat gcccgcggcg agtatgtggt tgccaaggcg
     5641 atcacgggta ggttgaatcc ggttcaagtg aaagacggtt cctgaagcga gacgcaactc
     5701 aaagttacga agttgaggcg gtggggttat atccttaaac caataaggag agacaaatgc
     5761 ctcgtaaagt caaattgatc ctcaacccta tggcagatat ggggcgggcg tggaagaccg
     5821 ccaatgcgtt gcggccgatt gctcaggaat ttcaaggtga gcttacctgg agcggaacgg
     5881 tgtacccaac acacgcgatt gaacttgcgc ggcaggctgc cgaagaggga tatgatatgg
     5941 tgattgccat gggcggggat ggtacggttc atgaagtgat gagcgggttg atgcaagtgc
     6001 ctgccgagaa gcgccctgtg atgggcgttg tgccgattgg gtctggaaat gattttgcgt
     6061 attctattgg tatccaccaa aagccagacc atgcgcttgc ccatgccttg aaggcagaaa
     6121 atattaaacc tgtggatata gggctgatga cggatgaaca cgggcgcaag gaatattttg
     6181 ataataccct tggcatcggt tttgacgcgg tggtaactat ccgttcgcat aagttgccga
     6241 ttgtcaaagg atttctgatg tacttgaccg cggtaattca aaccattctt ttgaatcata
     6301 accctgcaaa attgaatatt gaaactgatc atcaatcgtg gagcgcaagc gctttgatgc
     6361 tgacgctgtg taacggtccg cgagagggtg gtgggtttat gctttcgccg aaatcaaa
//
LOCUS       DAOQNJ010000080         6407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6796_length_6407_cov_10.239295, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000080 DAOQNJ010000000
VERSION     DAOQNJ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6796_length_6407_cov_10.239295"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..498
                     /locus_tag="PLG52_03710"
     CDS             175..498
                     /locus_tag="PLG52_03710"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNB40581.1"
                     /translation="MPRTIRKILRELKKGLTQIYGDQLNAVYLFGSYARGEGRIPDSD
                     IDVMVVLKDDFNRREVETRSIEFVASLCLEYDAVIMCKFITAKKYSDSKMPFMINVRR
                     DAVAI"
     gene            495..875
                     /locus_tag="PLG52_03715"
     CDS             495..875
                     /locus_tag="PLG52_03715"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNB40582.1"
                     /translation="MTTPEIKKLIAHAEESHQAAKVLLDGGFFGFSAAQSYYTLFYLT
                     EALLHSKGLQFSSHSAVIAAYGREFSKRAVLDPKYHRRLIVAESRRETGHYGEESSVT
                     DEEARESFEWAGEFLSVTKVYFGI"
     gene            903..1034
                     /locus_tag="PLG52_03720"
     CDS             903..1034
                     /locus_tag="PLG52_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40583.1"
                     /translation="MTDKTDIVNDQPTDTDALDFTPYVQTLADIIQTGNTPLTNDVL"
     gene            complement(1050..2426)
                     /locus_tag="PLG52_03725"
     CDS             complement(1050..2426)
                     /locus_tag="PLG52_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40584.1"
                     /translation="MGNTKSERDYFINSGLREIALLRKYFNIDGIGDKVKVTKLVSPK
                     NVVGFYREYFASVRPSNLSKDKTLPRSDNNKLRGLYLGQPDVNIVLNLIVRHCLYVDQ
                     IVLIDPFMSPVSFGVLDMPEGWMEVLINRALCLCALEEWIKQNIVIIIPDPIYFRPEV
                     TKIIEENASQFMEARDDNKDWKFMVVRALVNEEEEHREAFLDLLERMGQNFSVEERTS
                     LLYEASEFEKKYPIRFKLSKEFYKKYFDDVNKHGQVLDFSTGTSLIAAPYIAEAMGAF
                     LLFEHRHHFDEIRNKFKENQSSGSSRLQKIAIAFQQLDFPFLHNVPLTEALELRQKGY
                     LSRFRVYLKDLWDLSSSKLQEKDLNDQFQEFSDRLKSEYEVVQQEWGNIKKELRAKAV
                     ASGLIIGMSAGSALVFGNIAIPLSTLSGLGAGLLKEYFFGGYSETSEKVAQTTNQPLF
                     VFLTLENK"
     gene            complement(2516..5101)
                     /gene="gyrA"
                     /locus_tag="PLG52_03730"
     CDS             complement(2516..5101)
                     /gene="gyrA"
                     /locus_tag="PLG52_03730"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNB40585.1"
                     /translation="MELGIVRKIDIDTEMQQAYLDYAMSVIVSRALPDARDGMKPVQR
                     RILYAMYDMGIRSDSPYKKSARIVGEVLGKYHPHGDSSVYEAMARLAQDFSMRTMLVD
                     GQGNFGSVDGDPPAAMRYTEARLTAAALDILNDLNKNTVDFTPNFDDTLEEPSVLPSA
                     IPNLLVNGATGIAVGMATSIPPHNLSEVVDALVFMLERWEKLDDIDVEQLMKFVKGPD
                     FPTGGLIIEEKGDEGIEAAYGSGRGRITVQAKAHVEEMERGKNRIIVSELPYMVNKSS
                     LIERIAELARDGHLEGLSDLRDESDRQGMRIVLELTKNADPNFVLRDLYKRTPMQTTF
                     SINLLALVDGEPRTLTLKQALRVYLEHRLEVIKRRAQFDLEKAKARQHILEGYLVALK
                     NLDEIIELIRSAPDVEAARAKLMKRYKLTEIQANAILDMQLRRLAALERKKIETEYKE
                     VTAVIKELTTLLKTPKLMRGVVADELQKVKAAYGDRRRTQVVSVKEGGTAKALTTNDL
                     LPQQTVWVGVTDDGLVSRTADDKQPKHSGNDAPRFLVKASTTDTVYFVSKLGKAAAVA
                     AHLIPEAGKLSQGVPWHRVSPLGDTDALAAVFALPASKSALGEEVCVLTATRFGMIKK
                     SLVTELPGPSSQTFVLVKVNEGDKLNTVGITDGKKDILLITASGMAIRFKEDDVRPMG
                     LVAAGVNGMKLEDKDEVVGMEILPADGEIFLLTNEGKAKRVDQKDFPVQGRYGKGVVA
                     WDMPGKSTLAAVASGKPNHVATIHMTKGAPKSARLDAAGLRKRASAKGDVVVEVKPGE
                     GITSIAVGWMLESYVKVAKEEPKRKEGKKKETKAPVKKAAKAKAKPAKTSSPKGKAKP
                     VKKKK"
     gene            5293..>6407
                     /locus_tag="PLG52_03735"
     CDS             5293..>6407
                     /locus_tag="PLG52_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNB40586.1"
                     /translation="MTLITDSKTAPAPYKNDPRIEKDENGTWHVRGYNEARDLLKEDL
                     IQDGFNAEELRKAGFDGVLYMSGDPHRQMRAAIAKYFSPTTVQNTHMTVIEKTADDLL
                     SRLQARKRLNLKELTLEMATVIATAVVGLDPTPGLIQRLNAMLHAPKMSENKFIATLQ
                     HYEGHYRRFAFFFADVLPAIRRKRKNPGNDVITYMISKNKKPLEIWVECIVYGAAGMA
                     TTQEFILVVLLHCMKDAKLRETMLSDNAAARYEALNEILRLEPVIGKLYRKPKEPVSV
                     TSEGREYTIPAGERVTFHLYDVNADAKAVGEAPEKLNPHRQLEKGVYRSMMGFGSGPH
                     RCAGEHLALAETDVFIQKLLKLPGIRIESGPNIIRNDT"
BASE COUNT         1624 a         1697 c         1438 g         1648 t
ORIGIN      
        1 caagccagca tggaggcagt caataacagg aaggcgatgc gtttgagggt catggaaaaa
       61 cacctttaat cgattggaaa atgttggggc aattgtaaat caggtttgtg cgggtacaat
      121 atgggaacat ttctcaggat tgattgacgt ataatttagt caataggttt cattatgccg
      181 agaactatca gaaaaatact ccgcgaattg aaaaagggac tcacgcagat atacggcgac
      241 cagctaaacg ccgtgtatct cttcgggtcg tatgcacgcg gcgaagggcg tatccctgat
      301 tcggatattg acgtgatggt tgtcttgaag gacgatttca accgcaggga ggttgagacc
      361 cgttcgatag aatttgtggc ttcgctgtgt ctcgaatatg acgccgtcat tatgtgcaaa
      421 ttcatcacag ccaaaaaata ctctgatagc aaaatgcctt ttatgataaa cgtccgcagg
      481 gatgctgtgg caatatgaca acccctgaaa tcaaaaaact catcgctcat gcagaggaaa
      541 gccaccaggc cgcaaaggta ttgctcgatg gcggtttttt tggcttttct gccgctcaat
      601 cttattacac gctattctat ttgaccgagg cattgctgca ctcaaagggt ttacagtttt
      661 caagccactc ggcggtcatt gcagcttatg ggcgggaatt ctcgaagagg gctgtccttg
      721 accccaaata tcatcgccgt ttgattgtgg ccgaaagccg ccgcgaaaca ggccactatg
      781 gcgaggaaag cagtgtgacc gatgaagaag cacgggagtc ttttgaatgg gcgggggaat
      841 ttctttccgt tacaaaagta tattttggta tttgatgctt gacgcctgaa agggagaaat
      901 ttatgaccga caaaaccgac attgttaacg accagcccac cgacacggat gcgctcgatt
      961 tcacacccta tgtccaaacg ctcgcggaca tcatccagac cggcaatacg cctttgacca
     1021 atgacgtgct ttaaagcact ctaagcttat tatttatttt caagtgttaa aaacacaaaa
     1081 agtggttggt ttgttgtttg cgcaaccttt tcggaagtct cagaatatcc accaaaaaaa
     1141 tattctttta gcagtccagc gcctaaccct gaaagagtac taagtgggat agcgatattc
     1201 ccaaaaacaa gtgctgagcc agctgacata cctattatca atccactcgc aactgctttg
     1261 gctcgtaatt ccttcttaat gtttccccat tcctgttgta caacttcgta ttcagacttt
     1321 aacctatcgc taaattcctg aaattgatca tttaagtctt tttcttgcaa tttacttgag
     1381 cttaaatccc ataaatcttt taagtagact ctaaatctac ttaagtaccc tttctggcgt
     1441 aattctagtg cttctgtcaa tggcacatta tgcaagaaag gaaaatccaa ttgctggaaa
     1501 gcaatcgcta ttttctgcag cctagaactt ccactacttt gattttcctt aaacttattt
     1561 ctaatctcat caaaatgatg tctatgttca aataataaaa aagcacccat tgcttcagct
     1621 atatatggag cagcaattaa tgatgtaccc gtactaaaat ctaatacttg cccatgttta
     1681 ttaacatcat caaaatactt tttgtaaaac tctttagaaa gcttaaatcg aattggatat
     1741 ttcttttcga attcagacgc ctcatacaac aagctagtcc gttcttcgac agaaaaattt
     1801 tgccccatcc tctctaataa atccaagaat gcctcacgat gttcctcttc ctcattaact
     1861 aaagcccgaa caaccataaa tttccaatct ttattgtcat ctcttgcttc cataaattga
     1921 gaagcatttt cctcaataat cttcgttact tctgggcgaa aataaatcgg atctgggatt
     1981 atgataacta tattttgttt gatccactct tctaatgcac ataaacaaag cgccctatta
     2041 attaatacct ccatccaacc ttcaggcata tccaaaactc caaaacttac tggactcatg
     2101 aatgggtcaa tcaaaactat ttggtctaca tacaaacaat gacgaactat taaatttaaa
     2161 acaatattca catctggttg ccctagatat aatcctctta atttattatt atcagagcga
     2221 ggaagggttt tatctttact taaattactc ggacgaaccg aggcaaaata ttctctgtaa
     2281 aagccaacta catttttagg tgaaacgagt ttagttacct taactttgtc accaatacca
     2341 tcaatattga aatatttacg cagaagggct atttctctta aaccgctatt gataaaataa
     2401 tcccgttcgc tcttagtatt tcccataata tctgcctttc taaaaactat atattaagtt
     2461 gattaacaag gcaagtaaaa tcaaggctgg caaaacaacg ccagccttgc ctccattatt
     2521 ttttcttctt caccggcttt gccttcccct tcggggacga tgtcttcgct ggtttcgctt
     2581 ttgccttagc agcttttttc acaggagcct tcgtttcttt cttctttcct tctttcctct
     2641 tcggctcttc cttcgctacc ttgacataac tttccaacat ccaccccacc gcaatggagg
     2701 taatgccctc acccggcttg acctccacca ctacatcgcc tttggcagag gcgcgtttgc
     2761 gcagaccggc agcatccaag cgggctgact tcggcgcgcc cttggtcatg tggatggtcg
     2821 caacgtgatt tggcttgcca ctcgcaaccg ccgccagcgt ggatttgccc ggcatatccc
     2881 aggccaccac acctttgccg taacggcctt gcacggggaa gtctttctga tccacgcgct
     2941 tggctttgcc ttcgttggtg agcaggaaga tctcgccatc ggctggcaaa atttccatgc
     3001 caaccacttc gtccttatct tccaacttca tgccgttcac gcctgcggcc acgagaccca
     3061 tcgggcgcac gtcatcttcc ttgaaacgga tcgccatgcc gctggccgtg ataagcagaa
     3121 tatctttctt tccgtccgtg atgccaaccg tgttgagctt gtccccttcg ttgaccttga
     3181 ccaatacaaa tgtctgcgaa gatgggccgg gcaactccgt gacgagactc ttcttgatca
     3241 tgccaaaacg cgttgcggtc aacacacata cttcctcgcc cagcgcagac ttgctggcag
     3301 gcaaagcgaa gacagctgca agcgcatccg tgtcacccaa cggtgagacg cgatgccacg
     3361 gcacaccctg actcaactta cccgcttcgg ggatcaagtg cgcagccacc gccgcggctt
     3421 tgccaagttt gctcacaaag taaaccgtat cggtagtgga tgccttgacc aggaagcgcg
     3481 gcgcgtcatt gccggaatgc ttgggttgtt tatcgtccgc ggtgcgggaa accagaccat
     3541 catcggtcac accgacccac acggtctgtt gcggcagtaa gtcattggtg gtgagcgcct
     3601 tggcggtgcc gccttccttc acactgacaa cctgcgtacg acgacggtcg ccataggcgg
     3661 cttttacttt ttgaagttca tccgccacca caccgcgcat cagcttcggc gttttcaaca
     3721 gcgtggtcaa ttccttgatg acggcggtca cttccttgta ttcagtctcg atcttcttgc
     3781 gttcaagcgc ggcaagacgg cgcagctgca tatcgaggat cgcattcgcc tggatctctg
     3841 tcagcttgta gcgcttcatc agtttggcgc gggcagcctc aacatcgggt gcagagcgga
     3901 tgagttcaat gatctcatcc aaattcttca aggcgacgag atacccttcg aggatatgct
     3961 gtcgcgcttt ggctttttcc agatcaaatt gcgcgcgcct cttaatgacc tcaagcctgt
     4021 gctccaaata gacgcggagt gcctgtttga gagttaaagt gcgcggttcg ccatccacca
     4081 gcgccagcag attgatgctg aaggtggtct gcatcggagt gcgtttgtaa aggtcacgca
     4141 aaacgaagtt gggatccgca ttcttggtca gttccaggac gatgcgcata ccctggcgat
     4201 ccgattcatc acgcaagtct gaaagacctt cgagatgacc atctcgtgcc aactctgcaa
     4261 tgcgctcgat caaactggac ttgttgacca tgtacggcaa ttcagagacg atgatgcgat
     4321 tcttaccgcg ttccatctcc tcaacgtgcg ctttcgcctg caccgtgatt cgtccacgtc
     4381 ccgagccgta tgcagcctca atgccctcat cccctttttc ttcgatgatg aggccgccgg
     4441 tcgggaagtc gggtcccttc acgaacttca tcaactgctc gacgtcaatg tcgtcgagtt
     4501 tctcccagcg ttcgagcatg aacacgaggg cgtccacaac ttcacttaag ttatgaggcg
     4561 ggatcgatgt cgccataccc acagcgatac ccgtcgcgcc gttcaccaaa agattcggaa
     4621 tcgcggaagg aagtacactc ggttcttcga gtgtgtcgtc gaagttcggc gtgaagtcca
     4681 ctgtgttttt gttgaggtca ttcaaaatat cgagtgcggc agcagtgagg cgcgcctcgg
     4741 tataacgcat cgccgcgggc gggtcgccat cgacagaacc gaagtttccc tgaccatcca
     4801 cgagcatggt gcgcatcgaa aaatcctgcg ccaggcgagc catggcctca tacacagacg
     4861 agtcaccgtg cgggtgatat ttacccagca cctcacccac gattctcgcg gacttcttgt
     4921 atggcgagtc gctgcggatg cccatgtcgt acatcgcgta caagatgcgg cgctggaccg
     4981 gcttcatgcc gtcacgggca tcaggcaacg cgcgcgacac gatgacagac atggcgtagt
     5041 cgagatatgc ctgctgcatt tcagtgtcga tgtctatttt tcgtacgata ccaagttcca
     5101 tgtaaattcc tttttgaacc gctaagaccg caaagggctc aaagaagaaa agatcttctt
     5161 cgcggtcttg gggtgtgtag tggtattttt atggtgaatt aatgttctat tgcggggctt
     5221 atcttacggg caaacaaacg atgcgtcaag tgtattgtat aatggacaca ccaactttag
     5281 agagaggctg aaatgaccct tatcaccgat tcaaagaccg cccctgctcc atataaaaac
     5341 gacccacgca ttgagaaaga tgaaaacgga acctggcatg tgcgtggata taacgaagcc
     5401 cgcgatctgc ttaaggaaga tctgatccag gatggcttca acgccgaaga gctgcgcaaa
     5461 gccgggttcg atggcgtgct ctacatgagc ggcgacccgc accgtcagat gcgcgctgcc
     5521 atcgcaaaat atttcagccc cactactgtt caaaataccc atatgaccgt catcgaaaag
     5581 acagccgatg acttgctctc gcgcctgcaa gcccgcaagc gactcaacct caaagagttg
     5641 actctcgaaa tggcgaccgt catcgccacc gccgtggttg ggctggaccc tacacccggt
     5701 ctgattcaac gcctgaacgc gatgctgcac gcgccgaaga tgtctgagaa taaattcatc
     5761 gccaccctgc aacattacga agggcattac cgtcgcttcg ccttcttctt cgccgatgtg
     5821 ttgcctgcca tccgccgcaa acgcaagaac cccggcaatg acgtgatcac atacatgatc
     5881 tcaaagaaca agaaaccgct cgaaatctgg gtggagtgta tcgtctatgg cgcggcaggc
     5941 atggcaacca cacaggaatt tatcctggtc gtactgctgc actgcatgaa agatgccaaa
     6001 ctgcgtgaga cgatgctatc ggataacgcc gccgcccgtt acgaagcgct gaacgaaatt
     6061 ctgcgcctcg aaccggtgat cggaaaactg tatcgtaagc ccaaggaacc cgtcagcgtg
     6121 accagtgaag gcagggaata caccatcccg gctggcgaac gcgttacctt ccatctttat
     6181 gatgtcaatg cggatgccaa agccgtgggc gaggcaccag agaagttaaa tccgcatcgc
     6241 cagcttgaaa aaggagtcta ccgctccatg atgggcttcg gttctggtcc tcaccgttgt
     6301 gcgggtgaac acctcgccct cgccgaaacc gatgtcttca tccaaaagct tctcaaactc
     6361 cccggcatcc gcatcgaaag cggaccgaac atcatccgca acgatac
//
LOCUS       DAOQNJ010000081         6392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6816_length_6392_cov_10.196307, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000081 DAOQNJ010000000
VERSION     DAOQNJ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6816_length_6392_cov_10.196307"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="PLG52_03740"
     CDS             <1..1618
                     /locus_tag="PLG52_03740"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNB40587.1"
                     /translation="NFTFVFLLFVLMIGITGCNLPLPTCATDSLQEVTLLSPAKMGVV
                     DTLEPTLEWSYPSSECTPEGYAITMWEGPVMTENIGGGTGNPNTQWVPGSPLEPGKQY
                     RWTVAPINGTTLGPVSETFVFFTGPVCTDASLVPPTMLEPADGASFNATTDLLWWDYT
                     AGCTPTGYQLDVATDSSFASGLYPSGIIDIPLTRAVLGDPPLVECQTYYWRVAARNGD
                     TIGSYSPTRSFVADTTGTCGGSGGGGGGTPPDSASISGIVWHDICAIPDGPLPDPLSA
                     GCVEDGAGSARANGIREEGEPGIAGVQVNLYYGDCDFAGASDASVLTDADGRYIFDGL
                     TSFGNFCVAVNPGTSPNDAILLPGEWTYPDLITPDGVAYQTFGGIESGVHVDIIDFGW
                     DYQFLPAFNDPTPTPVAGNLTPNIPNFVDDSGFNTPLPQFVFDKNAFCRKGPNINYSD
                     VTAISAGDTVQIQGISSDRLWYHIFWAKFNVNCWVAGSTGHVVGDVQNIPVMTPPPTV
                     VPSSVPSSVPSNNNKNNTPTPVPSVTPVKP"
     gene            1823..3079
                     /locus_tag="PLG52_03745"
     CDS             1823..3079
                     /locus_tag="PLG52_03745"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB40588.1"
                     /translation="MNKRRTRPNWFRLALLSLLVLGAAYVNRFVVTNVDPLGVPSPTP
                     TISAETLSDQGDELFKQGKLVPAIDAYNQAIVSQPDNPALHITLARTLAFAGKYKEAQ
                     TAAEDALLLNPNNSMAHAVRAWALNFQEQYAEAESSITRALELDPNNALAHAYYVEIL
                     VDSYYSGLGAFDGIEKAIEESKFAQALAPDLLETHRARGYILEATGNYEEAIREYDQA
                     ISINPNISDLYLATGRNYRILGIYDKAVDAFTRADALNPEDPNPDLLLSRTYATIGEY
                     GKAMQFAESAVANNPADTNLRGNYGVMLYRNAYWEDAIRELSYVVKGGLSEDGHEMEV
                     INLVPDAPRIAEYYFTYGLALARTDNCGEALQISQLIISRVPADELAVNNANEIVNNC
                     TENLNAPPATATEEPVATEVDATPTP"
     gene            3076..>3737
                     /locus_tag="PLG52_03750"
     CDS             3076..>3737
                     /locus_tag="PLG52_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB40589.1"
                     /translation="MNIYNKRSLFRRRDESSVYRMFFVVIFILGGIWLIRSVQQGDVK
                     PLFLPTPTPTRFATSYTMEGDANFTAGQLNAAIEAYQEAATIDPSNADVWAQLSRVQT
                     YSTALIVQREDLLKRLDEAIVSAEKAIEVNPDSSNAHAVYAFALDWKASYTSDSRERQ
                     TLLQDAEQHATRAILLDNTNVLAQAFYAEILVDQQKWTQAQQFIDQALAADPTLMDVH
                     RV"
     assembly_gap    3738..3837
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3838..4655
                     /locus_tag="PLG52_03755"
     CDS             <3838..4655
                     /locus_tag="PLG52_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB40590.1"
                     /translation="IDQALAADPTLMDVHRVHAYVLESLGEYALAIEAYDKAIAIAPN
                     MTFLYLRAGAGYRRLAFESANDEVQRQLFEKSLEYFAQTARINSQLGVKDPAPYISIA
                     KTYSQMGEFFIAIRNIQKAIEFEPANAVLYGELGVLYHKNRNYETGILALGCAINGCT
                     GPESCDGRGGCGPNDVEAEVKPLALDGSTVYYYDILASELAALSTPKNNKCPQALEVA
                     AIIENSEYISDPNIATDMAVVRTICSFSPETTNTGSEPTPTAESATPEVTPTP"
     gene            4905..5207
                     /gene="groES"
                     /locus_tag="PLG52_03760"
     CDS             4905..5207
                     /gene="groES"
                     /locus_tag="PLG52_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNB40591.1"
                     /translation="MAKNNVSFKPLGSRVLIETIEQEEITASGIILPETAKEKPQQGK
                     VLAAGPGDRNDKGERVAMDVKTGDVVLFAKYSGTEVKMDGKKLLILRESDILGIVG"
     gene            5243..>6392
                     /gene="groL"
                     /locus_tag="PLG52_03765"
     CDS             5243..>6392
                     /gene="groL"
                     /locus_tag="PLG52_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNB40592.1"
                     /translation="MAAKQLVFSEDARRKLKNGMNVVANAVSATLGPKGRNVAIDRKF
                     GSPTITHDGVSVAKEIELEDPFENMGAQLLKEAAQKTNDIAGDGTTTSTVLAHAIVNE
                     GLKALEAGYNPMLLKRGIELATETIVAELKKNSTKIDTKEEIASVATNSAADEEVGQL
                     IADVMDKVGKDGVITVEESKTMQFETEFVEGMQFDRGYLSPYFITNADQMEAQIGDAY
                     VLIYDKKISAAQDIVPLLEKLVQLGKRELVIIAEDVDGEALATLILNKIRGMLNVLAI
                     KAPGFGDRRKAMLQDIAVLTGGEVISEEMGRKLESVTVQELGRAEKVVSDKENTTIIG
                     GKGKKANIEARIKEIRIEIDKSTSDYDREKLQERLAKLSGGVAIIRVGA"
BASE COUNT         1625 a         1497 c         1577 g         1593 t
ORIGIN      
        1 aaattttact tttgtgttcc tgctttttgt tttgatgatc ggaataacgg ggtgtaattt
       61 accgctgcct acctgcgcta cagatagttt acaagaagtc actctgctta gccctgcaaa
      121 gatgggtgtt gtagatacgt tagaacccac acttgagtgg agctatccat cttctgaatg
      181 tacgccagaa gggtatgcca ttacgatgtg ggaaggaccc gtgatgactg agaacattgg
      241 tggtgggacg ggcaacccca acacccagtg ggtaccaggt tcaccccttg aacccggcaa
      301 acaatatcgc tggacggttg cgcccatcaa cgggacgacg ctaggaccag tttcagaaac
      361 gtttgttttc tttacaggac cagtgtgtac cgatgcatct cttgttcccc ccactatgct
      421 tgagccagcc gatggagcaa gtttcaatgc aacgacagac ctattatggt gggattacac
      481 ggcagggtgt acgcctacag ggtatcaact ggatgtcgca acggattcat cttttgcaag
      541 cggactatat ccatctggga ttattgatat tccactaaca agggctgtgt tgggcgatcc
      601 tccattggtg gagtgtcaaa cctattactg gcgtgttgcc gcaagaaacg gcgatactat
      661 tggctcgtat tcgccaacgc gtagttttgt cgctgatact actggtacgt gcggtggtag
      721 tggcggtggc ggcggtggaa ctccacctga ctcagcttct atcagcggaa ttgtttggca
      781 tgatatttgt gccatacctg atggaccgct tcctgacccg ctttcagctg ggtgtgtaga
      841 agatggagct ggttctgctc gtgcaaatgg aatccgcgaa gaaggcgaac ctggtattgc
      901 aggggtacaa gtcaatttat attatggcga ttgcgatttt gctggagcaa gtgacgcaag
      961 cgttctaaca gatgccgatg ggcgatatat atttgacggt ctcacttcat ttgggaattt
     1021 ttgtgttgcg gttaacccgg gcacatcgcc taacgatgcc atcttactcc ctggcgaatg
     1081 gacttaccct gatctgatta cgccggacgg tgtggcatat caaacctttg gtggcataga
     1141 gtctggtgtg catgtggata ttattgattt tggttgggat tatcaattcc taccagcttt
     1201 taatgaccct acacctactc cagttgcagg aaatctaacg ccaaacattc ccaattttgt
     1261 agatgacagt ggttttaata ccccactgcc acaatttgtt ttcgataaaa atgctttctg
     1321 tagaaaagga ccaaatatta actatagcga tgtcactgct atttctgctg gtgatactgt
     1381 gcagattcaa ggcatcagtt ccgatagact ttggtatcac attttctggg cgaagtttaa
     1441 tgtgaactgc tgggtggcag gctctacagg gcatgtggtc ggtgacgtac agaatattcc
     1501 ggttatgact ccaccgccta ctgtggtgcc ttccagcgtg ccttcgtcgg ttccgtctaa
     1561 taacaacaaa aataataccc ctacaccggt gccaagtgtg accccggtga agccataagc
     1621 aaaatttctt agtattaata aacggacctc tgaagccaac ttcacaggtc cgtttatttt
     1681 gagaatgcat attttccgtc aaaaacatgg cagaaatgtg atttttgcta agaagcaaat
     1741 tagaagcgaa aacccctgct tgacgttgct ttctgccgga tggtaatatg attttatgta
     1801 tttacgagga agtaagtgga gtatgaacaa aaggcggacc cgcccaaact ggttccgcct
     1861 tgcattgctt agcctgttgg tgcttggtgc agcctatgtc aaccgttttg tggtgacgaa
     1921 tgttgatccc ttaggcgtac cttctccaac gccgactatt tcagccgaaa cattgagcga
     1981 tcaaggggat gaacttttca agcagggcaa acttgtccct gcgattgatg catataacca
     2041 ggcgatcgta tctcagccag ataaccctgc gcttcacatc accttggcgc ggactttggc
     2101 atttgctggc aaatataaag aagctcaaac tgctgcagaa gatgcgttat tactcaaccc
     2161 taataattca atggcacatg ccgtgcgtgc gtgggcactg aattttcaag aacaatacgc
     2221 tgaagcagaa tctagtatta cccgtgcttt agagcttgac cctaataatg ccttggcgca
     2281 tgcctattat gtagagattt tggtcgattc atattacagt ggtttgggcg cctttgatgg
     2341 catagagaaa gcgattgaag aatccaagtt tgcgcaggca cttgcgcctg atttgcttga
     2401 aacccatcgt gctcgtggat atattctgga ggcgacggga aattacgaag aagctatccg
     2461 tgaatatgat caggcgattt ctatcaaccc aaatatttcg gacctctacc ttgctacagg
     2521 aagaaattat cgtattctcg gaatttacga caaggcagtg gatgctttta cgcgtgccga
     2581 tgcattgaac cctgaagacc ccaatccgga cctgttactc tcccgcacct atgccacgat
     2641 cggtgaatac ggtaaagcca tgcaattcgc tgaatcagca gtggcgaata accctgcaga
     2701 taccaacttg cgcggaaatt atggcgtgat gctgtatcgt aatgcctatt gggaagatgc
     2761 tattcgtgaa ctttcatatg tggtcaaggg tggactttcg gaagatggtc acgaaatgga
     2821 agtgatcaac cttgtgccag atgcgccgcg tatcgccgag tattatttca cttatggttt
     2881 ggcgcttgct cgtacagata actgtggcga ggcattacaa atttcgcagt taatcatttc
     2941 gcgtgttcca gcagatgaac ttgctgtgaa caatgccaac gaaatcgtca ataattgcac
     3001 cgaaaacttg aatgctcctc ctgctacagc gacagaagag cctgtagcta ctgaagtaga
     3061 tgctacccca accccatgaa tatttataac aaacgcagtt tattcaggcg acgcgatgag
     3121 tccagcgtat atcgcatgtt ctttgtcgtc atcttcattc tcggcggaat ttggctaatc
     3181 cgctcggtgc agcagggcga tgtaaagccg ctctttttgc ccacgccaac tcccacccgt
     3241 ttcgctactt cctacaccat ggaaggcgat gcaaacttta cggcagggca gttaaatgct
     3301 gccattgaag cttatcaaga agctgccaca atcgacccaa gtaatgctga tgtttgggca
     3361 cagctttcgc gtgtgcaaac atactctact gcattgattg ttcaacgcga agacttgtta
     3421 aaacggttgg acgaagcgat tgtttcagca gaaaaagcga ttgaggtgaa cccagatagt
     3481 agcaatgcgc atgcggtata tgcatttgca ctagattgga aagcttctta tacatcagat
     3541 agccgtgaga gacagacgtt gcttcaagat gcagaacagc acgctacacg tgccatcctc
     3601 cttgataaca cgaatgtgtt ggcacaggct ttctatgcag aaatccttgt cgatcagcaa
     3661 aaatggacac aggcacaaca gttcatcgat caggcattgg cagctgaccc cacattgatg
     3721 gatgtgcacc gtgtgcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntca
     3841 tcgatcaggc attggcagct gaccccacat tgatggatgt gcaccgtgtg catgcctatg
     3901 tgttggaatc tttgggcgaa tatgcgctcg cgattgaagc atatgataaa gcaatcgcga
     3961 ttgcaccaaa tatgacattt ctttacctgc gggcgggggc agggtatcgc cgtcttgctt
     4021 ttgaaagcgc taatgacgaa gtgcaaagac agttgtttga gaagtcgctt gaatattttg
     4081 cacagacggc tcgtatcaat tcgcagttgg gtgtgaaaga cccagcgccc tatatttcga
     4141 ttgccaagac ctattcgcag atgggtgaat ttttcattgc tattcgtaat atccaaaaag
     4201 ccattgagtt tgagcctgct aatgccgttt tgtatggcga gttgggcgtg ttgtatcaca
     4261 agaaccgtaa ctatgaaacc ggtattctcg ccttagggtg cgcgattaat ggttgtacgg
     4321 gacctgagtc ttgtgatgga cgcggtggct gtggtcccaa cgacgtggaa gctgaagtga
     4381 agccattggc attagatggt agtacggttt attactacga tatccttgct tctgagttgg
     4441 cggcgttgag taccccgaaa aataataaat gccctcaggc gctagaggtt gcggcaatta
     4501 tcgaaaactc tgaatatatt tcagacccga acattgctac ggatatggct gtggttcgaa
     4561 cgatctgttc gttctctcct gagacgacaa acaccggctc tgaaccaacg ccgacggctg
     4621 aatcagccac ccctgaggtg acgcccacgc cttgattgac gacagggtcc tagcgcctgc
     4681 cagagccagg gtacagcggg gataaggctc gggtaggcga ggcagaaagc gggtccagaa
     4741 agaggcgggc gaagcgtcat cagagaaatg taagaatttt tatatatccc cttgacgttg
     4801 actttttaac tgtgtaagat agactttagc actcagttgt attgagtgct aattctgtga
     4861 ttatcctaaa aaatcaaaag tatcaacctt acaggaggca tcaaatggca aagaacaatg
     4921 tttcgttcaa acccctcggc agccgcgtgc tgatcgagac cattgaacag gaggaaatca
     4981 ccgcaagcgg gatcatcctc cccgaaaccg cgaaggaaaa accgcagcag ggcaaggtct
     5041 tggctgctgg tcctggtgac cgcaacgaca agggcgagcg cgtcgccatg gatgtaaaga
     5101 ctggtgatgt agttttgttc gccaagtatt ctggcaccga agtgaagatg gatggcaaga
     5161 aattgctcat tctgcgcgag agcgatattc tcggcatcgt cggctaatcc caaacaaatt
     5221 tctagatata aggaagtgaa ttatggcagc taaacaactg gtattttccg aagacgcccg
     5281 ccgcaagcta aagaacggca tgaatgtcgt tgcaaatgcg gtatccgcta ctcttggtcc
     5341 taagggacgc aatgtagcca tcgatcgcaa gtttggttcc cccaccatca cccacgatgg
     5401 cgtgtctgtg gccaaggaaa tcgaactcga agatccgttt gaaaacatgg gtgctcagct
     5461 tctcaaggaa gccgcccaga agaccaatga tattgccggt gacggtacca ctacctccac
     5521 tgttctcgct cacgcgatcg tgaacgaagg tttgaaggct cttgaagctg gttacaaccc
     5581 catgctcctc aagcgcggca ttgaactcgc caccgaaacc atcgttgccg aactcaagaa
     5641 gaactccacc aagatcgata ccaaagaaga aattgcttct gtcgccacca actctgcggc
     5701 agacgaagaa gtcggtcagc tcatcgccga cgtgatggac aaggtcggta aagacggcgt
     5761 catcaccgtt gaagaatcca agacgatgca gttcgaaacc gaattcgttg aaggtatgca
     5821 gttcgaccgc ggttacctct ccccctactt catcaccaac gccgaccaga tggaagccca
     5881 gattggcgat gcctatgtgt tgatctacga caagaagatc tccgctgctc aggacatcgt
     5941 tcctttgctc gagaaactcg tccagctcgg caagcgcgaa ttggtcatca tcgctgaaga
     6001 tgtcgatggt gaagctctcg ccaccctcat tctcaacaag atccgcggca tgctcaacgt
     6061 gctcgctatc aaagcccctg gctttggcga tcgccgcaag gccatgcttc aggatattgc
     6121 tgtcctcacc ggcggcgaag tgatctctga agaaatggga cgcaagctcg aatccgtcac
     6181 cgttcaggaa ctcggtcgcg ctgagaaggt cgtttccgac aaggaaaaca ccaccatcat
     6241 cggtggcaag ggcaagaaag ccaacatcga agcccgcatc aaggaaatcc gcatcgagat
     6301 cgataagagc accagcgact atgatcgcga aaagctccag gaacgcctcg ccaagttgag
     6361 cggcggcgtc gccatcatcc gcgtgggtgc tg
//
LOCUS       DAOQNJ010000082         6364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6876_length_6364_cov_7.867174, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000082 DAOQNJ010000000
VERSION     DAOQNJ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6876_length_6364_cov_7.867174"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLG52_03770"
     CDS             complement(<1..518)
                     /locus_tag="PLG52_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB40593.1"
                     /translation="MNSDSHRQLRNVVADKMRAAILDGQYKPGEWLRQERLAQELGVS
                     QMPVREALNELVNEGLIEHVPYRGARVIQFSVEDVIDLYEHRAFLEGRAAYFSAQVIT
                     DKDLMTLRKIQAGMEENDAPEAVLKYRELNRNFHKTIFTISKHKYLIRTLNQMWAAFP
                     TMLIGNFAATAS"
     gene            complement(528..1535)
                     /locus_tag="PLG52_03775"
     CDS             complement(528..1535)
                     /locus_tag="PLG52_03775"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNB40594.1"
                     /translation="MKLVFPFFRFTTLFIILVSLISPSPVFAAKKHYEYYVVGNSNNA
                     TSPTQAGTVLMGGGTDVDAAFQWMINKSGGGDFVVIRASGTDAYNPYIYGLGTVDSVE
                     TLIITSRTGASDPFVLDKINNAEALFIAGGDQADYVNYWKGTPVEDAIHNLIARNVPI
                     GGTSAGLAILGEFSFSAANGTVDSSTALGNPFGRRIALERDFLTVPYLSSLITDSHFV
                     TRDRMGRLVTFLARIVNDGWATQAKGLGIDEETAVVVEADGSASILGNGAAYFLQTPG
                     TPEVCLPKTDLTYRNVSVYRISGSATFNFATWTGSGGTAYTITAENGTVTSSQPGGSI
                     Y"
     gene            complement(1841..2572)
                     /locus_tag="PLG52_03780"
     CDS             complement(1841..2572)
                     /locus_tag="PLG52_03780"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB40595.1"
                     /translation="MRKPLFLAVMILIMLACAVPGAPAVAPLPTFDPNSLQTFIVQTA
                     EAAQTQTALVMPTFTKTPTTTFTPSITPSSTPTFIFKLSTPTPIPTYTIPPTIGTLKS
                     PVPESASGDDEENTDGDKPKNKDEDPRKMSGKDWTCVWYGLNPPRNTIFKPKTHFTVQ
                     WMLFNSGNQSWPRTGVDFVYTGGFRHEETKIQDFAQSVAPGKEIWVRASFIAPKAAGD
                     YSTYFTLTVGKRRFCSVAYYFTVQE"
     gene            complement(2585..4012)
                     /locus_tag="PLG52_03785"
     CDS             complement(2585..4012)
                     /locus_tag="PLG52_03785"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNB40596.1"
                     /translation="MNNVRPRLSLMNEEQIQEAHRNVLKVLSETGVRVDSPEILQMLE
                     WKLGLKSQDRIIKFPPAVVEEAIKSAPKTIDVYDRRGEHKLKLGEDRLRFGVGVTALF
                     YQNPVDETLDIFKRENFRDLVRLASGLKHYDVISTVGIVRDVKEELGDMYGSLEHISN
                     TTKPLVLLVSDEDRFPDVLDMFEHLNGKEVGDKPFVIPYFNPVSPLVMNAGTVDKMKV
                     AIERGLPIIFSNYSMAGASTPLTPAGTLTLLMAELLAGLVISQTIKKGAPILLGMLPV
                     YFDMRTMLNFYDPQSILISVACSEMMKSYGIPHCSTSGSGTGWGMDLIAADTYWMNTL
                     ALLLSNGHLAPFIGDSLGSKSISPTTFVHAHEIIDQALRLHNGFQLDDANSAVDEIFK
                     VGPGKSFLNQPSTLKNYKNGYYVSGVYPRYSMEKWMDAGAPPARQVLRQKTQDLMASA
                     PVPNEYDEFIAKGEEFIRKRFHDIL"
     gene            complement(4087..5100)
                     /locus_tag="PLG52_03790"
     CDS             complement(4087..5100)
                     /locus_tag="PLG52_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HNB40597.1"
                     /translation="MSKNQLHDYFDELDDLSEGAQDSARKRTSIKRESKRKEGEAKLF
                     IRAQEAAKEFKFTYKAARFEEAWLLDSLTEIYEHQWISDVLRKVKGGKEASVYLCKSG
                     TDMSVPFVAAKIYRPRSLRNLKNDAQYRVGRVDLDADGSALWKEADINAIAKRTTYGE
                     EVRHRSWIAYEFKTMEKLFEAGVDVPKPYAMEKNAIVMDFIGDANTAAPILNSVNLSA
                     HEAKNLFDRVIHNIDLMLANDCIHGDLSAYNILYWNGAITLIDFPQIVLPKSNPTAWN
                     IFQRDVIRICDYFSAQGIHCDGRKIAAELWTFRGYRIGKQADPKYLDPEDPADRKLWE
                     KQQ"
     gene            complement(5347..6246)
                     /locus_tag="PLG52_03795"
     CDS             complement(5347..6246)
                     /locus_tag="PLG52_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB40598.1"
                     /translation="MTETSLQRVDPKSLTAFILVVLLGGSNSVAIRFSNLELAPFWGA
                     FLRLMPAALIYWAILLFRKMDLPTFKDSAVIAINGFISTGVGFALLYWGLQTVPVSLA
                     TVVISTGPLFTLVLAVLHRLESFRIQSLIGGLIAFSGLAIAINAQPGGRELIPGIIAL
                     LIGALISAEGNIIFKIYSVNSNPVSINALSLFSAAVFLGITSFFTQETWNLPSTSAGW
                     TALAYLIIFGSVLMFYLFVYVLTRWTASSASYSILLFPLVATVFAALLANETITLPFI
                     LGGVIVLLGVWVGAFYTKPKTGA"
BASE COUNT         1713 a         1445 c         1597 g         1609 t
ORIGIN      
        1 tgtgatgctg tggcagcaaa attaccgatc aacattgttg ggaatgccgc ccacatttga
       61 tttagggtac ggattaagta tttatgcttg ctgattgtaa aaatagtctt gtgaaaattc
      121 cggttaagct cacgatactt caataccgct tccggcgcat cattctcttc cattcccgcc
      181 tgtattttcc gtaaggtcat caagtcttta tctgtaataa cttgtgctga aaaataagca
      241 gctcgccctt ccaggaaagc gcggtgctca tacaaatcaa taacatcctc aacagaaaat
      301 tgaataacac gcgctccccg atatgggaca tgttcgatca gcccttcatt gacaagctca
      361 tttagagctt ctcgcacagg catttggctt actcccaact cctgtgcgag gcgttcctga
      421 cgcagccatt ctccgggttt gtattgccca tcaagaatgg ctgcacgcat tttatctgcg
      481 acaacgttcc gtagctgtct gtgagaatct gaattcatag ctccgtctta gtaaatggat
      541 ccaccgggtt gggaagatgt cacagtgcca ttttcagcag tgatcgtata tgctgttccg
      601 ccgctgcctg tccatgttgc gaagttgaat gtggctgagc cgctaatgcg ataaacagaa
      661 acattacgat aggttaggtc ggttttaggt aggcaaactt caggcgtgcc aggagtttga
      721 aggaagtacg cagctccgtt tccaagaata gaggcgctcc cgtcagcttc aacaactacg
      781 gcagtttctt catcgatgcc caaccctttt gcctgtgttg cccatccatc gttgacgata
      841 cgagcaagga atgtaacgag tcgacccata cggtcacggg taacaaagtg actatccgtg
      901 ataagactgc tcaaataagg gacagtaaga aaatcacgct cgagagcaat acgacgacca
      961 aatgggttgc ctaaagccgt ggaagagtct actgtgccgt ttgctgccga gaaagaaaat
     1021 tctcccaaaa tggcaagccc ggcacttgtg cctcctatag gcacgttccg tgcaatcaga
     1081 ttatgaatcg catcttccac tggagtccct ttccagtagt taacataatc agcctggtcg
     1141 cctcctgcaa taaacagagc ttcagcatta ttgatcttat ccaacacaaa ggggtcactt
     1201 gcgccagtac gtgatgtaat aatgagagtc tctacagagt caaccgtgcc taaaccataa
     1261 atataaggat tataggcatc tgtacctgaa gcgcgaatga caacaaaatc tcccccacct
     1321 gatttattga tcatccattg aaatgctgca tcaacatcgg tgccaccgcc catgaggacc
     1381 gttccggctt gagtaggtga agtggcattg tttgagttac caacaacata gtattcataa
     1441 tgtttttttg ctgcgaaaac ggggctaggg gatataagcg acaccaagat gataaacaag
     1501 gtggtgaatc gaaaaaatgg aaatacaagc ttcattttat gctcctttgg catagcacgt
     1561 tattgaaggt gggtatttct aaattagata taatatctag gtttaggata gcgtttattg
     1621 gttgatgtgt caatactttt taagaggaat aatttgatat ttattgacta tgcgcaattt
     1681 aaggagtgcc cttaatacat gataatttga ctgcctcatt acgaattttt gtaactagca
     1741 caaaaaagag accctatgct tcaggatctc tttaaaatca atgtttccct cccccattcc
     1801 atagagtata tttagcgcaa gttattgtct atgcgcaatt tcactcttga actgtaaagt
     1861 agtatgccac agaacaaaat cttcttttgc caaccgttaa agtaaagtat gtagagtaat
     1921 caccggcagc tttgggagca ataaaagatg ctcgtaccca aatctctttg cctggggcaa
     1981 cactctgggc aaagtcttga atctttgtct cttcatggcg gaacccgccg gtataaacaa
     2041 agtcaacacc ggtgcgcggc caagactggt taccactatt aaacaacatc cactgaaccg
     2101 taaagtgggt ctttggtttg aatatcgtat tcctaggcgg attcaaccca taccaaacgc
     2161 aggtccaatc tttgccagac atctttcttg ggtcttcatc tttattcttt ggcttatcac
     2221 catctgtgtt ttcttcatcg tcaccagatg cagactctgg aacaggagat ttcaaggttc
     2281 cgatcgtagg cggaatggta taagttggta tcggtgttgg agtagaaagc ttaaaaataa
     2341 aggttggagt agaactcggt gtaattgacg gtgtaaaagt ggtggtggga gttttagtaa
     2401 aggtaggcat caccaaagcg gtttgagttt gagccgcttc tgccgtttga acgataaagg
     2461 tttgaaggga atttgggtca aatgttggca aaggggcaac agctggcgca ccggggacag
     2521 cacaagccaa cattattaaa atcatgacag cgagaaaaag aggtttacgc acgggaactc
     2581 ctacttaaag tatatcgtga aacctttttc ggataaactc ttctcccttt gcaatgaact
     2641 catcatattc gttaggtaca ggcgcggatg ccattaaatc ctgggttttt tggcgcaata
     2701 cctgacgggc cggcggcgca ccggcatcca tccacttttc catgctgtag cgcggataga
     2761 ctccacttac atagtatccg ttcttgtaat ttttcaaagt cgaaggctgg ttgaggaaac
     2821 tcttgccggg acctactttg aaaatctcat ctactgcaga gttggcatcg tctaattgaa
     2881 agccattgtg gaggcgtaag gcttggtcta tgatctcgtg tgcgtggacg aaggtggttg
     2941 gggagatcga cttcgagcca agagaatctc cgataaacgg agccaggtgt ccgttagaga
     3001 gaagaagcgc gagggtattc atccaatagg tgtctgccgc gataaggtcc atgccccagc
     3061 cggtgccact accagaggtg gagcaatgcg gaatgccgta ggacttcatc atctcagaac
     3121 aagcaacact aatgagaata ctttgcggat catagaagtt gagcatagtg cgcatgtcaa
     3181 aatagacagg caacatgcca agcaaaattg gtgcgccttt tttgatagtt tgactaatga
     3241 ctaaacctgc aagcaattct gccatcagga gcgtgagcgt gcccgctggc gttaatggcg
     3301 tggatgcgcc cgccatgctg tagttggaaa agatgatggg aagtccgcgt tcgatggcga
     3361 ctttcatttt gtcgacggtg ccggcattca tcacgagcgg actgaccgga ttgaaatacg
     3421 gaatcacaaa gggcttatcg ccgacttcct tgccattcaa atgctcgaac atatcgagca
     3481 catctggaaa gcggtcttca tctgagacaa gcaacaccaa tggcttggtt gtattggaga
     3541 tgtgctccaa tgagccgtac atatcgccaa gttcttcttt tacatcacgg acgatcccca
     3601 ccgttgagat gacgtcataa tgtttgagcc cagaagccaa gcgtacaagg tcgcgaaagt
     3661 tctcgcgttt gaaaatatcc agcgtttcat ccaccgggtt ttgatagaac agcgccgtca
     3721 cccccacccc aaagcggagg cggtcttccc ccaatttcag cttatgttcg ccgcgacggt
     3781 cgtaaacatc tatcgtcttt ggcgcggact tgatcgcttc ttcgacaact gcgggcggga
     3841 atttgataat gcggtcttgc gacttcaagc ctaatttcca ttcaagcatt tgcagaattt
     3901 caggcgagtc gacgcgcact ccggtttcgc tgaggacttt caacacattt cgatgtgctt
     3961 cttgaatctg ttcttcattc atcaaactca atcgcgggcg gacgttgttc atagatcatc
     4021 tcctattcaa attgtaatag aggaaggaag aaaaaagaag ccagtgcaat tgcactggct
     4081 ttgtacttac tgttgtttct cccacagttt gcggtctgcg gggtcttcgg ggtcgaggta
     4141 tttggggtcg gcttgtttcc caatgcgata accgcggaag gtccaaagct cggctgcgat
     4201 tttacgtcca tcacaatgaa tgccctgagc cgagaaataa tcgcagatgc gaataacatc
     4261 gcgttgaaaa atgttccatg cggttgggtt ggatttcggc aaaacaatct gcggaaagtc
     4321 gatcaacgtg atggcgccgt tccagtacaa aatgttgtat gcggataagt cgccatgaat
     4381 acagtcgttt gccaacatca ggtcgatgtt atggatcact ctgtcgaaca agtttttggc
     4441 ttcatgtgca gaaagattaa ccgaattcaa gatcggcgcg gctgtgtttg catcgccaat
     4501 aaaatccatc acgatggcat tcttttccat ggcatagggt ttaggaacat cgacaccggc
     4561 ttcgaaaagc ttttccatgg tcttgaattc gtaggcgatc cacgaacgat ggcggacctc
     4621 ttcaccgtag gtagtgcgtt tggcgatagc attgatgtcg gcttctttcc acaaggcaga
     4681 gccgtcggca tccaagtcca cgcgcccgac gcggtattgc gcatcattct tgaggttgcg
     4741 caacgaacgc ggacggtaga tcttcgcggc gacgaaaggc acgctcatat cggtgccaga
     4801 cttgcacaag tagaccgagg cttccttgcc gcctttgacc tttcgcaata cgtcagaaat
     4861 ccattggtgt tcgtagatct ctgtcagcga atccaagagc caggcttctt cgaagcgcgc
     4921 ggctttgtag gtgaacttga attctttggc ggcttcctgg gcgcggatga agagcttcgc
     4981 ctcgccttct ttgcgtttgg actcgcgttt aatagaagta cgcttgcgag ccgagtcttg
     5041 tgcaccttca ctcagatcgt ctaattcatc aaaatagtca tgtaattgat tcttactcat
     5101 tgttataaaa ccttatctct taacaaaaaa accacaggga ccttcctgcg gctggttgaa
     5161 tgaggttaaa caaaaaccgc aggctgcgag cctgcggttg aatcgatagg gcaagactat
     5221 gccaattcgc tcaacggagg cgcgccaaac aaaacaactg tggaaaccaa gatagcttgc
     5281 atgtttttga gcctcctttc gtggaataag atagtaaaga aattttatgc caagccccaa
     5341 gcaacgtcaa gccccagttt tgggtttggt gtagaaagca cccacccaca ccccaagaag
     5401 gacaatgact ccgccaagga taaagggcaa ggtaatggtc tcgtttgcca gcaaagcggc
     5461 gaagacggta gccaccaagg gaaaaagcaa aatggaataa gaagcggaag aagccgtcca
     5521 gcgagtgagg acgtagacga acaggtaaaa catgagcacc gagccaaaga tgatgagata
     5581 ggcaagggct gtccagccgg cggaggtgga gggtaaattc caggtttctt gggtaaaaaa
     5641 tgaggtgatg ccaagaaata cagcggcaga gaacaaggac agggcattga tcgagacggg
     5701 gttgctgttg acagaatata tcttgaaaat gatattccct tcggcgctga tgagagcccc
     5761 aatgagcaag gcgataatgc caggaattaa ctcacgcccg ccgggttggg cgttgatggc
     5821 gattgccaag ccagagaagg cgatcagccc accgatgagg gattggatgc gaaagctctc
     5881 gaggcgatgg agcactgcca gcaccaaagt aaataaaggt ccggtggaga tgacgacggt
     5941 ggcgagactc acaggaaccg tttgcagccc ccaatacagt aaggcaaagc caaccccggt
     6001 agagataaag ccattgatgg cgatgacggc ggagtctttg aaggtgggca gatccatttt
     6061 gcggaagagg aggattgccc agtagatgag cgcggcgggc atgaggcgga ggaaggcgcc
     6121 ccagaagggt gccagctcca agttactgaa gcggatggca actgagtttg aacctccgag
     6181 caaaacgacg agaatgaagg cggtgaggga tttggggtct acacgttgga gggaagtttc
     6241 ggtcatgggt ctcttttctt tagtagtagg ggcgacccgt catagcagta ctttagagaa
     6301 cgtttataag gaagggtcgc ccctacaaaa aatagtattc aacttggttg ggtcgcccct
     6361 actt
//
LOCUS       DAOQNJ010000083         6357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6896_length_6357_cov_8.517296, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000083 DAOQNJ010000000
VERSION     DAOQNJ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6896_length_6357_cov_8.517296"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLG52_03800"
     CDS             complement(<1..407)
                     /locus_tag="PLG52_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNB40599.1"
                     /translation="MWHNYINATSIDEVVRILAEKRESARVVAGGTDLVLELERGVRK
                     GVDTLIDVTRIPQLDQITIDEDDVIHLGALVTHNDCAASKLIRTRACPLARASWEVGA
                     PQIRNRGTVAGNLITGSPANDTITPLMALGAKVT"
     gene            complement(450..971)
                     /locus_tag="PLG52_03805"
     CDS             complement(450..971)
                     /locus_tag="PLG52_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40600.1"
                     /translation="MSDFFEKVSSQVDPFKKLVSFIPGFSGYIERQNRRDADKLLRET
                     VARRFEEQWTRASSLQVDMVNNGMLSYVDDMEKASLALRTFIDKISTAARGYSGMFDA
                     VKINEKELEAIYQFDAAFFDLADQVKSGLDNVEASMGDEAALPAAIRNITSLSRLAVE
                     TFERRSEIVTGSR"
     gene            1152..2570
                     /locus_tag="PLG52_03810"
     CDS             1152..2570
                     /locus_tag="PLG52_03810"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNB40601.1"
                     /translation="MSDHVKQLLDLVENSISFDGTTVRVLDAAKFRKNVGKLVERSAL
                     ASDSSAGLARFLVRAAALELGVYAASIHDLYMARGRGDAPMTWSTPAFNLRALSHHAA
                     RAMFRAANKINAGAFIFELARSEMNYTDQRPSEYATNILGAAVAEGYTGPVFIQGDHF
                     QVSAKKYKADAAAELKAVRDLSIEAMAAGFFNIDVDTSTLVDISLPTVQEQQTLNSKL
                     SAELSAFIRENEPAGVTISIGGEIGEVGTENSTEPELRAYMDAYNTNLQKLAPGKPGL
                     SKISVLTGTSHGGTVLPDGSIAEVSIAFDVLRDLSRVARKDYGMGGTVQHGASTVPEE
                     NFNKFVQNEAIEVHLATNFATMFFDNIPADFKKEIYAWLDVNAAGDRKPGMTDEQFYY
                     KVRKNAIGPFKAKSYALPAESLDKLGKAWEAQFDKLFNLLGLKDTKNQTEKFIKPVKV
                     APVLADYVKDAVAAEDVSDLSD"
     gene            2576..3070
                     /locus_tag="PLG52_03815"
     CDS             2576..3070
                     /locus_tag="PLG52_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB40602.1"
                     /translation="MPVSEQGVTKDRYMLIPRTAIFVRRGDEYLLLKGAPTKRLWAGK
                     YNGLGGHVDRGEDVLSSARRELMEEAGISADLWLAGTVIVDAGEIGILLFVVVGENPQ
                     GEVKGSEEGTPEWVQKEAVSQLPVVEDLPVLLERIHSMKKGDPPFAARSFYENDKLNV
                     VFGE"
     gene            complement(3100..3885)
                     /locus_tag="PLG52_03820"
     CDS             complement(3100..3885)
                     /locus_tag="PLG52_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40603.1"
                     /translation="MRQTHQLVITILVLVLLTACGGNEQSSNISLPTVPPPAGQTTSD
                     IVSQLCDNPLFPVKQNASWSYFSSGGPNGDFAYTDTITSLTPDGFVLTSQFPALSLTQ
                     NWICAPEGYIAQQLGGGTTASVSMQNMITDFKTIQVSGVSLPRTLTPGMQWKYDLSMQ
                     GAVAMPGEQTQSPGTFALTMQELGTETITVPAGTFDATKIQATFTANIDVDFEGSTIR
                     YTVNGTSLLWYAPGVGFAKSIENIDFSGTSFTSTTELQSYNIP"
     gene            complement(3891..5057)
                     /locus_tag="PLG52_03825"
     CDS             complement(3891..5057)
                     /locus_tag="PLG52_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB40604.1"
                     /translation="MTTLRTPARIIPRAFPGIKPEEVQEIIVNSQIKSYPAGTILCQE
                     DAIEHTFYMILEGDFEVTKTINNKEKRLLKTLIAGDFFGEMALIHNAPRAATVQSLTP
                     AVVLELDKQGFDRVLKHSPSVATAMVAEISNRLRANDQLAIEDLRLRAREIADAYQKL
                     AEQDMARNEFLTSIAHELRTPLMVASGYLHTLKKGVLTGEQLQSTIEIVSRNVDQIVN
                     LTNDLLFLQEQDLVLRDFQALNLYHVVEGVVEKYQAKAKTRSINIKVRGSANIPSVQG
                     DEPSLERAIAGLVDNAIKFSPYGGTVEIRFADLGNYVSVSVVDYGIGIDPQIRPRIFD
                     RFFHLEKSNDEIYDGLGIGLSITRQVIQQHRGMLDVDSQPGKGSTFTISLPKWK"
     gene            complement(5124..5354)
                     /locus_tag="PLG52_03830"
     CDS             complement(5124..5354)
                     /locus_tag="PLG52_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40605.1"
                     /translation="MNDLEKIIMEEVSTLDDMRLIDVIGFIRYLKAEKPIRQEWIAGW
                     YESAIKSMHEREEELRITPAELEKQLRKKQSQ"
     gene            5555..>6357
                     /locus_tag="PLG52_03835"
     CDS             5555..>6357
                     /locus_tag="PLG52_03835"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40606.1"
                     /translation="MEKPIERPSILQTYTRSFEPLKNANLRMYLGGQAISLLGTWMQS
                     TAQSWVVWELTQSESALGIVVMLNSLPLFLLAPWAGGFADRFNRRAILMVTQATAMLL
                     AFALAFLVQTKLVQIWHIYVFSFVLGTIAALDFPAQQAFIGDLSGMKQVRQAVTLNIM
                     GLQLARMLGPAAAGFLLKAVGSAAAFWINGASFLVVLFSIFMVKSHQQEIHRRENGRG
                     QFREVLDYVRAQPRMVDLLIFASLVTFFGLSIMNILPAVADDVLKGDSQT"
BASE COUNT         1530 a         1477 c         1585 g         1765 t
ORIGIN      
        1 gtaactttgg caccaagcgc catgagagga gtaatggtat cgtttgcggg agagccggtg
       61 atgaggttgc ctgctacggt tccgcgatta cgaatttgcg gagcgccgac ctcccaagac
      121 gcacgtgcca gcggacaggc tctggtgcgg atgagtttgg atgccgcaca gtcattatgc
      181 gtgaccagtg caccaagatg aataacatca tcttcatcaa ttgtgatttg atctagttgg
      241 ggaatgcgag taacgtcgat gagtgtatca acgcctttgc gaacgccacg ttcaagttca
      301 agaacaaggt ctgttccacc tgcgacgacg cgggcacttt cacgcttctc agcaaggata
      361 cgaacgactt cgtcaatgga tgttgcgttt atatagttgt gccacatatg aatatccgta
      421 ggggcggggt gacccgcccc tacataaact taacgactgc ctgtcacgat ttcagagcgg
      481 cgttcaaagg tttccactgc gaggcgtgag agactggtga tgttacgaat ggcggcgggc
      541 agggcggctt catcgcccat gctggcttct acattatcaa gcccgctctt aacctggtct
      601 gccagatcaa agaaggcagc atcaaattga taaatagctt cgagttcttt ttcgttgatc
      661 ttcacagcat cgaacatgcc agagtaaccg cgcgcagcgg tgctgatctt atcgataaaa
      721 gtacgcaacg ccagtgatgc tttttccata tcgtcaacat aagagagcat cccattgttg
      781 accatgtcca cttgcaggct tgaagcgcgt gtccactgct cttcaaaacg gcgcgctacg
      841 gtttcgcgaa ggagtttatc tgcatcgcga cgattttgtc tttcgatgta accactaaaa
      901 ccgggaatga aagacaccaa cttcttaaac gggtcgacct gactactaac tttttcaaaa
      961 aaatcgctca tgtgagcctc ctgccaaaat atacgtactc ggtcaagttt tgtttcacac
     1021 tgaaaaggtt gccggcaaca aggggctaaa atgacattta tccggtttta ttcaggcgcg
     1081 tcactgtggg taattatatc gtttaattcc agttataatc aagttatcaa tttttattta
     1141 ggagaaatag tatgtctgac catgtaaaac aattactcga ccttgtcgag aatagcattt
     1201 cgtttgacgg aactacggtc cgtgttttgg atgcagctaa atttaggaag aatgttggca
     1261 agttggtgga acgttctgcg ctcgcttcag actcctccgc gggcttggct cgttttctgg
     1321 tgcgtgctgc ggcgctcgaa ctgggcgtct atgctgcatc gatccatgac ctctatatgg
     1381 ctcgcggacg tggcgatgcc ccgatgactt ggtctacgcc agcgttcaac ttgcgcgctt
     1441 tgtctcacca tgcggcgcgt gccatgttcc gcgcagcgaa taaaatcaac gctggtgctt
     1501 tcatctttga attggctcgc tctgaaatga attacaccga ccagcgcccg tcggaatatg
     1561 caaccaacat tctgggtgcg gctgtagcag aaggatatac aggaccggta ttcattcaag
     1621 gtgaccattt ccaagtctct gccaagaaat acaaggctga tgcagctgct gaattgaaag
     1681 ccgttcgcga cctttccatt gaagcaatgg cagctggctt cttcaacatt gacgtggata
     1741 cctctactct tgtagatatc agcctcccga ccgttcagga acagcagacc cttaacagca
     1801 aactttctgc agaactttct gccttcatcc gcgaaaatga gcctgcaggc gtgaccatct
     1861 ccattggtgg tgaaatcggt gaagtgggaa ccgaaaattc caccgaacct gaactccgcg
     1921 cttatatgga tgcttacaat accaatcttc agaaattggc tcctggcaag cccggcttga
     1981 gcaaaatcag tgtgttgacc ggtacttctc acggtggtac ggttttgccc gacggctcaa
     2041 tcgctgaagt cagcattgca tttgatgtgt tgcgcgatct tagccgtgtt gcccgcaaag
     2101 attatggcat gggcggtacg gttcaacacg gtgcttcgac cgttccagaa gaaaacttta
     2161 acaagtttgt tcagaacgaa gcgattgaag ttcaccttgc gaccaatttt gcgaccatgt
     2221 tctttgataa catccccgct gatttcaaaa aggaaattta tgcttggttg gatgtcaatg
     2281 ctgctggtga ccgcaaacct ggtatgacgg atgagcagtt ctattacaag gttcgtaaga
     2341 atgctattgg accgttcaaa gcaaaatctt acgctctgcc tgcagagtct ttagataagt
     2401 tgggcaaggc ttgggaagct cagtttgata agcttttcaa cttgcttggg ctgaaagaca
     2461 ccaagaacca aaccgagaag ttcatcaagc cggtgaaggt tgctcccgtt cttgccgatt
     2521 atgttaaaga tgcggtggcc gcggaagacg ttagcgatct ctcagactaa aaaagatgcc
     2581 tgtctctgaa caaggcgtaa cgaaagatag atatatgctc attccccgca cggcgatttt
     2641 cgtccggcgc ggggatgagt atttattgct caaaggtgcg cccacaaaac gattgtgggc
     2701 tggcaaatat aatggcttag gtgggcatgt agatcgagga gaggatgtac tttcttcggc
     2761 aagacgcgag ttgatggaag aagctggaat ttctgccgac ctttggctgg ctgggacagt
     2821 gattgtcgat gcgggagaaa ttggtatttt gctttttgtt gttgtagggg agaaccctca
     2881 aggcgaagtg aaaggttcag aagaaggcac accagaatgg gtccagaaag aggcggtgag
     2941 tcaacttcct gtcgtggagg atctgccggt cttgcttgag cgcatccatt caatgaaaaa
     3001 gggagacccg ccttttgcgg cgcggtcttt ttatgagaac gataaattga acgtggtctt
     3061 tggagaatga aaacgccgcc taataaggcg gcgtttgttt tacggaatgt tataactttg
     3121 cagctcggtg gtggatgtaa atgatgtgcc gctgaaatcg atgttttcaa tggattttgc
     3181 gaagcccacg cccggtgcat accataataa acttgtgccg ttgaccgtgt accggatggt
     3241 acttccttca aaatcaacat cgatgttggc agtgaaggtg gcttgaattt ttgtcgcatc
     3301 aaacgtacct gctgggacag ttatggtttc ggtaccgagc tcttgcatag tgagcgcgaa
     3361 tgtgcccggg gattgagttt gttcaccagg catggcgact gcgccttgca tggaaaggtc
     3421 gtatttccat tgcataccgg gggtgagtgt gcgtggcagg ctgactccag agacctggat
     3481 tgtcttgaaa tcggtgatca tgttttgcat ggagacgctg gctgttgtgc ccccgccaag
     3541 ttgttgagca atatagcctt ctggggcaca aatccaattt tgcgttagag acagagccgg
     3601 gaattgtgag gtgagtacaa agccatctgg cgtaagactg gtgatggtat cggtgtaagc
     3661 gaaatcacca ttgggtccgc cgctgctgaa ataggaccag gatgcatttt gtttgacagg
     3721 gaaaagtgga ttgtcacaaa gctgacttac tatatcggaa gtcgtctgcc cggcgggtgg
     3781 tgggacggtt ggcaacgaaa tatttgaaga ttgctcattg cccccgcatg cagtgagtag
     3841 aaccagaact aggatagtga tgacaagttg atgagtctgg cgcatggaat ttatttccat
     3901 ttcggaagag agattgtgaa cgtgcttcct tttcccggct ggctgtccac gtctaacatg
     3961 ccgcggtgtt gttgaatgac ctgacgagtg atcgaaagtc cgatccccaa accatcgtaa
     4021 atttcgtcgt tacttttttc aagatgaaag aaacggtcaa aaatacgtgg acggatctgc
     4081 gggtcaatgc cgatgccata atccaccacg cttacactga cataattgcc taagtctgca
     4141 aaacgaattt ctactgtgcc gccatatggg ctgaacttaa tggcattatc gactaaccct
     4201 gcaattgcgc gctctagaga aggctcatcc ccttgaaccg atgggatatt ggcgcttcca
     4261 cgtactttga tatttatgct gcgagtcttt gctttggctt gatacttttc aacaacaccc
     4321 tctacaacat gatacaagtt aagtgcttgg aaatcacgta gcacaaggtc ttgttcttgc
     4381 aagaaaagca aatcattggt taagttcacg atctgatcca cgttgcggct gacgatttca
     4441 atagtcgact gcaactgctc gccagtgaga accccttttt ttagagtatg taaatatcca
     4501 ctggcaacca tcaacggagt ccgcagttcg tgggcgatgc tggttaggaa ttcattacgc
     4561 gccatatctt gctcagcaag tttttgatat gcatccgcga tctcacgagc gcgtaaacgt
     4621 aaatcttcga tagcgagctg gtcgttcgct cttaaccgat tgctgatctc tgccaccatt
     4681 gcggtggcta cgctgggact gtgttttaag actcggtcga agccttgctt atcaagttcc
     4741 agcaccacag ccggcgttag cgattgaacc gtggcggcac gtggcgcgtt atggattaac
     4801 gccatctcgc cgaagaagtc tcctgcaatc agagtcttaa gcagtctttt ttctttatta
     4861 ttaatggtct ttgtaacttc aaaatcccct tcgaggatca tataaaaagt atgttcaatc
     4921 gcatcttcct gacatagaat cgtaccagcc gggtaactct taatctggct gttgacgata
     4981 atctcttgca cttcttccgg tttgatgccg ggaaaagctc gtggaatgat tctggcaggg
     5041 gtgcgaagtg tggtcatgct tttggatatg gatcctacat tgattttagc acgggcagat
     5101 tatctgttat tcacccatta ggactattga ctttgctttt ttcttaactg tttttcaagt
     5161 tctgcgggag taatccttaa ttcttcttcg cgctcgtgca tagacttaat tgcactttca
     5221 taccagcctg caatccactc ctgccgaatg ggcttttctg ccttgagata gcggataaac
     5281 ccgatcacat cgattagacg catatcatcc aaggtggaaa cctcttccat gataattttt
     5341 tctaggtcgt tcataatagc tcctacactt tgaatacaaa cagtcattgg agaaatatat
     5401 aggatttcct cgacattgtt ttgaaaggaa atcggtttat agttaagggg atttcccaaa
     5461 ttgtactttg ttttattagt gattggtaag aattcagctt gggatttcat ccgaattctt
     5521 gtctaaaatc tcttcaatcc cttaggagcc ttccttggaa aaaccaattg aacgtccttc
     5581 cattcttcaa acctacactc gcagtttcga gccgcttaag aatgccaatc ttcgcatgta
     5641 cttgggtggg caagcgattt ctctgcttgg tacgtggatg caatctacag cacaaagttg
     5701 ggtggtttgg gaattgactc aatctgaaag cgcgcttggc atcgtggtca tgctcaatag
     5761 tttgcctctc ttcttgcttg cgccatgggc gggtggcttt gcagaccgtt tcaatcgccg
     5821 cgcgattctc atggttactc aagccaccgc catgcttcta gcttttgcct tggcgtttct
     5881 agtgcaaacc aagcttgttc aaatctggca tatttacgtc ttttcattcg tccttggcac
     5941 catcgccgcg ttagacttcc ccgcccaaca agcatttatc ggcgatctct ctggtatgaa
     6001 gcaagttcgc caagcagtga cgctcaatat catgggcttg caacttgcgc gtatgttggg
     6061 tcccgccgcg gctggctttt tgctgaaagc cgttggttct gccgcagcct tctggatcaa
     6121 tggcgcgagc ttcctcgtgg tcttgtttag tattttcatg gtcaagtctc accagcagga
     6181 aattcaccgc cgcgaaaacg gacgtggaca attccgcgaa gtgttggatt acgtccgcgc
     6241 acagccgcgc atggtggatt tattgatctt cgcctctctc gtgacctttt tcggtctctc
     6301 catcatgaac attctccccg ccgtagccga tgacgtcctc aaaggcgact cgcaaac
//
LOCUS       DAOQNJ010000084         6349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_6906_length_6349_cov_10.029552, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000084 DAOQNJ010000000
VERSION     DAOQNJ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6906_length_6349_cov_10.029552"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..709)
                     /locus_tag="PLG52_03840"
     CDS             complement(65..709)
                     /locus_tag="PLG52_03840"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNB40607.1"
                     /translation="MSAAETLERIAHEVSVCTKCALHESRKKAVTGEGPADAEIMFIG
                     EGPGFHENEQGRPFVGASGKFLDQLLEQAGLTRSDVFIANVVKCRPPANRDPQPEELM
                     ACGEYLDGQIEAINPSIIVTLGRISMGRYFPGAKITSVHGQMQNIDGRFIIPMYHPAA
                     ALHQPALRPSILADFAKLPDQLNEARAALGRKVVEKKKNVVVQQPEKPQQLSLF"
     gene            complement(719..1177)
                     /gene="rimI"
                     /locus_tag="PLG52_03845"
     CDS             complement(719..1177)
                     /gene="rimI"
                     /locus_tag="PLG52_03845"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNB40608.1"
                     /translation="MAEVLIRKMIHADLEQVIEIDQISFSLPWPKRSFQYELDENFTS
                     RCWVADVDGRIAAMMVGWMIIDELHVATIATHPDYRGQGIGKKVLVHALLAARKEGVI
                     RAFLEVRQTNEAALNMYRSLGFVEDGRRKEYYKDNNEDAILMSLNDMTHL"
     gene            complement(1183..1848)
                     /gene="tsaB"
                     /locus_tag="PLG52_03850"
     CDS             complement(1183..1848)
                     /gene="tsaB"
                     /locus_tag="PLG52_03850"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNB40609.1"
                     /translation="MLLAVDTSTALVGLALYDGDQVLGEMTWTTKQHHTTELSPALSG
                     LLKRCGVTMTSVNALGVAIGPGSFTSLRVGLSLVKGIALARNLPVMGISTLDVIAATQ
                     PISDTPLVAVLQAGRTRVAYGVYQSVDGQWQAEGPVRSGTLDEMLSQFESATLIAGEL
                     TSEERKKVFKAKHFQLASPVLCVRRPAVLAELAWARWQDDDADDAVSLAPIYLHVAGT
                     PIQ"
     gene            complement(1851..2345)
                     /gene="tsaE"
                     /locus_tag="PLG52_03855"
     CDS             complement(1851..2345)
                     /gene="tsaE"
                     /locus_tag="PLG52_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNB40610.1"
                     /translation="MSILDAHMLEFFSRSPEQTRRIGMRLGSLLEAGDVICLQGNLGA
                     GKTTFTQGLAQGWGSLDTVSSPTFILVNQYRRADGAQLFHLDAYRLETVPEAEELDLD
                     SMLAEGALIIEWPERLGDLIPAEHLHIRLEHLSEEHRQMNFHAHGKRYDDLLDSIRQS
                     MVGG"
     gene            complement(2423..3745)
                     /locus_tag="PLG52_03860"
     CDS             complement(2423..3745)
                     /locus_tag="PLG52_03860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB40611.1"
                     /translation="MDPQNTDLPIGSLRIGLFTDTYAPQVNGVSVSLQMISEGLKKRG
                     HQVTIFAPKFPGYKDNEPNVMRLPSLKYLNNPPIYVAVLGTPRSTWKLTRKHFDVLHA
                     HSPASVGLLAYLTASTKRLPLIYTYHTSITDYTHYIKFIGNTGIIKYAASWFSKASTD
                     LGDQIIVPSPKFYRLLLNQKVKQPISIIPNGIDLSMFKVAKNPGALRKRLGLGADVPI
                     MLTVGRMDPEKRLDFIVEAFDLIADRVPNAHLVFAGDGGARKHVEEKANSTRAKDRIH
                     FLGMVNRADLPDILHDANVFLSASTTEVHPISVIEAIASGLPFVAVQDEAFEGMLEEG
                     QNGYTVPLDVKKYADVLADLLPDRERLQRFSAHSRELSEKYSIEAQVKALEKLYMEAI
                     LQNWRGTLFKRMIPRQLNEIPQRISKEINEFPQKITKEINKILPGKKD"
     gene            complement(3769..3996)
                     /locus_tag="PLG52_03865"
     CDS             complement(3769..3996)
                     /locus_tag="PLG52_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HNB40612.1"
                     /translation="MLPDLQMNDHLRLRKPHPCGSYEWTVVRLGADIGLECKGCQHRV
                     MLTRRELTKRMKTNLSQQEREKHRDSEEHNT"
     gene            4202..5155
                     /locus_tag="PLG52_03870"
     CDS             4202..5155
                     /locus_tag="PLG52_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB40613.1"
                     /translation="MNKDFTVKFWGVRGSYPTPGVSTVQYGGNTACVEVNAGGRTIIL
                     DAGTGIIPLGRELAKRQNLELLILFSHLHHDHTQGFPFFVPAYMPKAKLHIYGPGGTA
                     ETVKHVLEHNQSSDTFPISLRDMASNKDIQSLRETQVIVWDEDGVSVHEASPSLSPEA
                     VVIRIHKSYAHPGGVYHYRIEYQGKSIVYATDTEGYVGTDRKLVNFTRNADVLIHDAQ
                     YSDDHYYGCLAGFPATQGYGHSTATMAGQVAASAETGQLILFHHDPAYADDWVAAQER
                     AAKNIFPDSLAAFEGMTLALTPQPLSQRARGVKEVKYVTHG"
     gene            5139..5846
                     /locus_tag="PLG52_03875"
     CDS             5139..5846
                     /locus_tag="PLG52_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB40614.1"
                     /translation="MLRMVELKKDDTRNLLSDIRLFEGLAPAQLDWVAHRAHRRVFPT
                     GKNVMLIEQPGEAVYVILHGTVKVYTEQGGRDAILSILGSGDLIGEMSLIDSVGRSAS
                     VVTLEDSLMLWMDKTAFFYMLDNFPPVARNLVKILSARVRLSDQMIQSLATLDVNGRV
                     ARQLLAFAERYGRDMDGGVKIRIALTQSDIADLVGASRKRVNQAMVFFKEQGLATTDT
                     DGHIIIQDKAGLAKYCD"
     gene            5988..>6349
                     /locus_tag="PLG52_03880"
     CDS             5988..>6349
                     /locus_tag="PLG52_03880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB40615.1"
                     /translation="MTDYNFFHPTEVRYGDLDPQGHVNNAKYLTYFEQARVYYFMKLG
                     LFSKDQSFMEIGVIVADIHITYHAPTHYGDNIKAGAKVTKIGNKSMTVEQCIVNADTG
                     KVMASGEVVLVTFDYEGL"
BASE COUNT         1612 a         1617 c         1530 g         1590 t
ORIGIN      
        1 ttggcattct tttctttcaa tttcttgatt aaatcttcct tgatagacat ttttaccttc
       61 aatttcaaaa tagacttaat tgttgtggct tttcaggttg ctgaacaacc acattttttt
      121 tcttttcaac aactttcctt cccaatgcag cacgggcttc gttaagttga tccggaagct
      181 ttgcaaaatc tgccaaaatg gatggacgca aagctggctg atgaagcgct gccgcggggt
      241 gatacatggg aatgataaac cttccgtcaa tattttgcat ctgcccatgt acggaagtaa
      301 tctttgcgcc ggggaaatat cgccccatgg aaatacgccc caacgtcaca atgatactcg
      361 gatttatagc ttcgatctga ccatccaaat attcaccaca tgccattaat tcttcaggtt
      421 gcgggtcacg gttcgcaggc ggacggcact tgaccacatt cgcaatgaac acatccgaac
      481 gagtcaaacc ggcttgttcc aacaattgat ctaaaaactt acccgatgcc cccacgaacg
      541 gacgtccctg ttcattctca tgaaatcccg gaccctcccc aatgaacatg atctcagcat
      601 ccgctggacc ttcacctgtc actgctttct tgcgcgactc atgcaaagca cacttcgtac
      661 aaacagaaac ttcatgggca attcgttcaa gggtttcagc ggcagacatg cagtctcctt
      721 acagatgcgt catatcgttc aacgacatca gaatggcatc ctcattatta tctttgtaat
      781 actcttttct tcgaccatct tccacaaagc ctaaacttcg atacatgttc aaagccgctt
      841 catttgtttg gcgcacttca agaaaggcgc ggatcacccc ttcttttctc gccgctaaaa
      901 gggcatgaac cagtactttc tttccaatgc cctgcccgcg atagtcggga tgcgtagcaa
      961 tcgtcgcgac atgcaattca tcaatgatca tccagcccac catcattgca gcaatacgac
     1021 catccacgtc agccacccaa caacgcgacg taaagttttc atctaattcg tattgaaagg
     1081 aacgcttcgg ccacgggagt gaaaaagaaa tctgatcgat ctcgatcacc tgttccaaat
     1141 ctgcatggat catcttacga attagaactt cagccataat ctttactgga tcggtgtgcc
     1201 tgctacatgc aagtaaatcg gcgcaagcga cacggcatca tcagcgtcat catcctgcca
     1261 acgcgcccaa gccaactctg ccaatacagc cggtctgcgc acacacaaaa ctggagacgc
     1321 caactggaaa tgttttgcct tgaagacttt ctttctctct tcggatgtta actccccagc
     1381 gatcaaagtc gcactctcga actgactcaa catctcatcc agggttccgc ttcttacagg
     1441 accctcagcc tgccactgac catccacact ttgatacacc ccgtatgcga ctcgcgtccg
     1501 cccggcttgc aagactgcca ccaatggggt gtctgaaata ggctgtgttg cggcaattac
     1561 atccaatgtt gagatgccca tcactggcag attacgagcc aaggcaatgc ccttcaccaa
     1621 agacaacccg actctcaaac tcgtgaatga accgggtccg attgctacac ccaaagcgtt
     1681 gaccgaagtc atggtcacgc cgcagcgttt caaaagtccg gaaagagccg gggaaagttc
     1741 cgtggtgtga tgctgtttgg tggtccacgt catctcaccg aggacttgat ccccatcata
     1801 caaagctaac ccaaccaatg cggtggaagt atctacggca agcaacatag ttaacctccc
     1861 accatagact gacgaatgga gtctaagaga tcatcgtagc gtttgccgtg cgcgtgaaaa
     1921 ttcatttgac ggtgttcttc tgaaaggtgc tcaagccgaa tatgcaaatg ctcagctggg
     1981 atgagatcgc ccaaacgttc gggccactca atgatgagtg cgccttcagc gagcatggag
     2041 tcgaggtcga gctcctcagc ttcgggcacg gtttcgagcc gataggcgtc gaggtgaaaa
     2101 agttgagcgc catcggcacg gcgatattga ttgaccagaa taaacgtggg gctagaaacg
     2161 gtatctaacg acccccaacc ttgtgccaga ccttgtgtga aggtggtctt gcctgcgcca
     2221 aggttgcctt gtaaacaaat aacatctccc gcctcaagca aactgccaag gcgcatgccg
     2281 attcttcttg tttgttcggg gcttctgctg aaaaactcca gcatgtgggc atcaaggatg
     2341 gacatcgggt tggaattata ccccccgctt tgctagttac aaattacggg ttacgcattg
     2401 tagatacgta acccgtaatc attcagtctt tttttcctgg caggatctta ttaatttcct
     2461 tggtgatctt ttgcggaaat tcattgatct ctttcgaaat tctttgagga atttcgttta
     2521 actgtctggg aatcatgcgt ttgaagagcg ttccgcgcca attttgaagg atggcttcca
     2581 tgtacagttt ttccaaggct ttgacctgcg cttcgatgga atacttttcg ctcagttcgc
     2641 gagagtgtgc gctgaaacgc tgtaaccgtt cacggtctgg gagcaagtcg gcaagtacat
     2701 cagcgtattt cttcacgtcg agcggaaccg tatagccgtt ttgcccttct tcgagcatgc
     2761 cttcaaaggc ttcatcctgc acggcgacga atggcaaacc ggaagcgatt gcttcaataa
     2821 cagaaatagg atgaacttct gtcgtagacg cggagaggaa cacattggca tcatgcaaaa
     2881 tatcgggtaa gtctgcgcgg ttgaccatac caaggaagtg gatacggtct ttggcgcgag
     2941 tggaatttgc tttttcctcc acatgcttgc gggcgccacc atccccggca aagacgaggt
     3001 gagcgtttgg tacacggtct gcaatcaagt caaaagcttc gacgatgaaa tcaaggcgtt
     3061 tctctgggtc catgcgacca acggtgagca taatcggcac atcggcacct aaccccagtc
     3121 tctttctcaa tgcgccgggg tttttcgcga ccttaaacat gctcaggtca attccatttg
     3181 gaataatact gataggttgt ttcacttttt gattcaaaag taaacgatag aacttcggag
     3241 aagggacgat gatctgatcc cccaagtctg tagatgcctt actgaaccat gatgccgcat
     3301 atttgataat gcctgtattc ccgatgaact tgatgtaatg agtgtaatca gtaatagacg
     3361 tgtgatatgt gtagatcaat ggcagtcgct tggtggaagc tgtaagatac gccagtagcc
     3421 ccacacttgc agggctgtga gcatgcagca cgtcgaaatg cttgcgcgtt aacttccacg
     3481 tagagcgcgg agttcccaat accgccacgt aaataggcgg attgttcaaa tatttcagcg
     3541 aaggcaggcg catcacattc ggttcattgt ctttatagcc ggggaatttt ggcgcaaaga
     3601 tggtgacttg atgtccgcgc ttcttcaagc cttctgagat catctgcaac gagaccgaaa
     3661 caccatttac ttgtggagcg taggtatcgg taaataagcc aatgcgaagt gaaccaatgg
     3721 ggagatctgt attctgtggg tccatgtatg ctccttgttt gtatttatct acgtattatg
     3781 ttcttctgag tctctatgtt tttcccgttc ctgttgagac aagttcgttt tcatgcgttt
     3841 tgtcagttcg cggcgggtga gcatgacacg atgctggcag cctttgcatt ccaaaccgat
     3901 atctgcgccg agccgcacca cggtccattc gtaagagccg cagggatgtg gtttgcgcaa
     3961 acgtaggtga tcgttcattt gcaggtctgg aagcatacaa aggattatac ctttacgacc
     4021 atagacgata gaccatgaaa aaccgtccat cgtccgcact tgggaaatag ttctcacctg
     4081 cccagagaac actttccttg aaggatacag agtttggaac ataatacaag tgtcttggag
     4141 gtgacaaagc ttcagaaaga agaccgtcat ctccaagaac ctcaacaaat tggaggcaga
     4201 catgaacaaa gatttcacag tcaaattctg gggcgtgcgc ggaagttacc cgacgccggg
     4261 tgtaagcacc gtccaatatg gtggcaacac ggcttgcgtg gaggtcaacg caggcgggcg
     4321 caccatcatt cttgatgcgg gcacgggcat tattccgctt gggcgtgagc ttgccaaaag
     4381 gcagaatctt gaattgctga ttctgttcag ccatctgcat cacgaccaca cgcagggatt
     4441 tccgttcttt gtgcctgcgt acatgccgaa agcaaaattg cacatttatg gtcccggcgg
     4501 cacggctgag acggtgaaac atgtgctgga gcacaatcag tcttcagaca cattcccgat
     4561 ctcattgcgt gacatggcgt cgaacaaaga tattcaatcg ctgcgtgaga cgcaggtcat
     4621 tgtttgggac gaggatggag ttagtgtcca tgaagcaagc ccaagtctga gtcctgaggc
     4681 ggtcgttatc cgcattcata aatcgtatgc gcatcccggc ggcgtctatc actatcgcat
     4741 cgaatatcaa ggcaagtcca ttgtgtacgc cactgacacc gaaggatatg taggcacaga
     4801 ccgcaagttg gtgaacttca cgcgcaatgc cgatgtgctc attcatgatg cgcaatattc
     4861 agacgatcat tattacggat gccttgctgg atttcctgcg acgcaaggat atgggcactc
     4921 gaccgcaacc atggcaggac aagttgccgc ctctgccgaa acgggacagt tgatcctgtt
     4981 ccatcacgac ccagcctatg ctgacgattg ggtggcggcg caagaacgtg ctgcgaaaaa
     5041 tatttttcct gattcactag cggcgtttga gggaatgaca cttgccctca ccccccagcc
     5101 cctctcccaa agggcgcggg gagtaaagga agtaaaatat gttacgcatg gttgaactca
     5161 agaaagacga cacccgcaat ctgctttcag acattcgttt gtttgaaggg cttgcccccg
     5221 cccaattaga ctgggtagct caccgcgcgc atcgccgcgt atttccgacc ggcaagaatg
     5281 tgatgctgat cgaacagcca ggcgaagcgg tgtatgtcat cctgcatggg accgtgaagg
     5341 tgtataccga acaaggcggg cgagatgcga tcctgtccat tttaggcagc ggagatctga
     5401 tcggcgaaat gagtttgatc gacagcgtag ggcgctctgc cagtgtggtg accctcgaag
     5461 actcgctcat gctttggatg gataaaaccg cgttctttta catgctcgat aactttccgc
     5521 ccgtagcgcg caatttggtg aaaatccttt cggcacgagt acgcctctct gaccagatga
     5581 ttcaatcgct tgccacatta gatgtgaacg gacgggtggc gcgtcaactg ttggcgttcg
     5641 ctgaacgcta cgggcgcgat atggatggcg gcgtgaagat ccgcattgcg ctcacccaaa
     5701 gtgacattgc tgacctggtg ggcgcctcgc gcaaacgggt gaaccaagcc atggtattct
     5761 tcaaagagca gggactcgcc accaccgata cagatgggca catcatcatt caagataaag
     5821 cagggttggc gaagtattgt gattagttga gtagtcgaat agtctcttag tcaaatagcc
     5881 gtggagcgtt ccgctctgcg gctatttttt agccatgcaa ccatctgacc attcgacgaa
     5941 caggctaaaa actacccaac tcagaattac caaactataa tcgcttcatg accgattaca
     6001 acttcttcca cccaaccgaa gtccgctacg gagatttgga cccgcaagga catgtcaaca
     6061 acgcaaaata cctgacgtat ttcgagcagg cgcgtgtcta ctatttcatg aagcttggtt
     6121 tgttcagcaa agaccaatcc tttatggaaa ttggcgtcat cgtggcagat attcacatta
     6181 cctatcacgc ccccacgcat tacggcgaca atatcaaagc cggtgcaaag gtcaccaaga
     6241 tcggcaacaa gtctatgacg gtagagcagt gcattgtgaa tgccgacacg ggcaaagtga
     6301 tggcaagcgg cgaagtggtg ctggtcactt tcgattacga aggtttgaa
//
LOCUS       DAOQNJ010000085         6282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_7032_length_6282_cov_9.380440, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000085 DAOQNJ010000000
VERSION     DAOQNJ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7032_length_6282_cov_9.380440"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLG52_03885"
     CDS             <1..395
                     /locus_tag="PLG52_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB40616.1"
                     /translation="HFVHTLPDGYNMVLNEETSNISQGQMQLLTIARAFLADPKILIL
                     DEATSSVDTRTEVLIQRAMTSLMKNRTSFVIAHRLSTIRDADLILVMNHGDIVEQGTH
                     KELLAKFGFYHELYMSQFKKKEDETIVA"
     gene            461..1801
                     /locus_tag="PLG52_03890"
     CDS             461..1801
                     /locus_tag="PLG52_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40617.1"
                     /translation="MTETTQTPPEEKLDFKRILPILVIVLVDLMGLSIIIPLLPLYAA
                     RFGAEPIVVGILQATYPLMQFIGAPVLGRLSDRFGRKPVLIASQIGTLIGFIMLGFAN
                     SMVVLFISRIIDGLSGANISTAQAAIADSTNEKTRTQGLGLVGAAFGVGFVLGPILAF
                     IVLAASGQNYQYVAWTAALFSLISILLTTFWFKETVADAANPEASRKRRPFSFGNLRE
                     AMAHRPTIMFLVIVMFFYQIAFGGYEQLFSLFTLNRLGMDARDTSGLFMLAGILIIVI
                     QGGLIGRWSRKKGDRWLALMGIGALAIGLIGTALTPAMPVPWYDKARVLESLAGQGEF
                     RVSIQSINVSLPDEAARGWFGIIWLMIASIPAALGGGTLQPAINSLITKDSDKSEVGG
                     MLGLSAAAYSAANAIAPLFYGALFQWFGAPVPFFAGGAILLALFLFAPRVIKNS"
     gene            complement(1830..2507)
                     /locus_tag="PLG52_03895"
     CDS             complement(1830..2507)
                     /locus_tag="PLG52_03895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNB40618.1"
                     /translation="MPLDVYRINALCFDVDGTLSDTDDLYVQKVSKLFPRFLFKDPEH
                     AARRFVMWIEAPGNALLGLADTLHLDDEMVAVINWLSRHRKQSDKKFLLVPGVDEMLK
                     QLHGRYPMAVVSARDEKGTMRFLEQYDLVKYFDAVITGLSAVHTKPYPDPILLAAQKM
                     NVSPENCLMIGDTTVDIRAGKSAGAQTLGVLCGFGEEPELRHLGADEIVEDTTKLLEI
                     LTINRER"
     gene            2551..4680
                     /locus_tag="PLG52_03900"
     CDS             2551..4680
                     /locus_tag="PLG52_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB40619.1"
                     /translation="MTEDPNRALLELLVKVSREVATALDLRTVLQRVLYACIQYVGGE
                     RGSIVVMDDLGKPVDATIVYGKQFHDHTTQQLRETVERGLAGWVVQNRKPALVPDTSK
                     DERWLRRADDAAEKSGAKSAICVPLLARERFVGVLTLVHPVPNAFNEQHLELVQAISD
                     QASIAVLNARLYTETQRTARVMTALAEGAATINSSLEMQDVLRRILNQTMQALQVETV
                     ALGLIDSHSNDLVFQAAAGNNSGHIPGLREPAGKGLVGKVIQAGRGVILPSIKHDPTL
                     VEEDRLNGVEMRALAIAPIQLHGKVIGVLEAVNPISGAFDPDALVVMTGLGNLAGTTI
                     DNSKLFEQLQNAHKHYRELFEDSIDPILVTDWEGKVLEANRQAVELSGYTTEQLHTMS
                     IDQLHEVNWGKVGLSFETIKAGTSCTYESGMLRQDGGRLPVEVYARRVEFEDSDSIQW
                     ILRDITARKDLDALRDDMTSMIFHDLRSPLGNIVSSLEMMSTLMPEDETLSSMLNIAK
                     NSTGRIQRLVNSLLDINRLEAGQRIVDQNSIDPVALVREAIRDVEPSVTARQHVLTNR
                     ALSVLPLIWVDVDMIHRVLINLLENSIKFTPAGGRIDIGAQTNEDGLFVRFWIRDNGP
                     GIPAAERERIFEKFTRLRGKNKAGGLGVGLAFCRLAVHAHDGDIWIESEEGKGTTFWL
                     TLPVAQRKSAGQIKRQTGRLTFKPDKP"
     gene            4740..5597
                     /locus_tag="PLG52_03905"
     CDS             4740..5597
                     /locus_tag="PLG52_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB40620.1"
                     /translation="MQEITKNIFIEDQFLGVTLGVIVTPRGLIQIDAPPSPEDARSWR
                     ASLMNLGGGIDRLLINMDAHPDRTLGARAMDCTVVAHEKTAYIFRTRPSTFKAQGEET
                     GADWESIPGLGSVRWAPPEISFVDQMTLHWGSSPVMLEHRPGPSGGSIWVNLPEEKVL
                     FVGDMVMKNQPPFLAGSNLKAWLESLDLLLEPEYKGYTFISSRGGVVNSSAIKAQYDF
                     LKHVHDKLNKSTSKKPNPAAVEKMVTSLLTWFKAPAARQKQFAQRLRYGLLHYNARQS
                     YAPSNTIED"
     gene            5597..>6282
                     /locus_tag="PLG52_03910"
     CDS             5597..>6282
                     /locus_tag="PLG52_03910"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNB40621.1"
                     /translation="MNTSLPLLELMRGPVVEATHLGSIAVVTADGTLLHSYGDPHTVA
                     FLRSSAKPFQVLPFVEAGGVEHYNYTQRELALSCASHETSQMHLDAVTALQKKVGIEE
                     HDLQCGPHLPGDARKLREVIQQNILPTANFNNCSGKHTSMLAFAKMRGLPLTNYLAID
                     HPIQQDILKTLSEMCNIDANEIQLGVDGCSAPNFALPLFHAALGMARMCDTRDLSESR
                     ATACKKITTA"
BASE COUNT         1525 a         1629 c         1573 g         1555 t
ORIGIN      
        1 atcatttcgt ccacaccctg cccgatggct acaacatggt cttgaacgaa gagacttcga
       61 acatctcaca gggacagatg cagttgctga cgattgcgcg cgccttcctc gctgacccga
      121 agatcctcat ccttgatgaa gcgaccagct cagtggatac acgcaccgaa gttctcattc
      181 aacgcgcgat gaccagcttg atgaagaacc gcacctcgtt cgtgattgct catcgcctct
      241 ccaccatccg cgatgcagat ttgatcctcg taatgaatca tggtgatatc gtggagcaag
      301 gaacgcacaa agaattgctc gcaaagttcg gcttctatca cgaactgtac atgagccagt
      361 tcaagaaaaa ggaagacgaa accatcgtag cgtaataaaa tcaacagacc ctaaagtttc
      421 gctttagggt ctgtcattaa atcaaatcca aggattgtcc atgactgaaa caactcagac
      481 tccccccgaa gaaaaacttg atttcaaacg catcctgccg attctcgtca tcgtgcttgt
      541 agacttgatg ggactttcca tcatcattcc gcttttgcct ttgtatgccg cccgcttcgg
      601 cgcagaaccg atcgtggtcg gcattctcca agccacgtac ccgctgatgc agttcatcgg
      661 cgctccagtt ctgggtcgcc tttcagaccg ctttggtcgt aagccagtcc ttattgccag
      721 ccagatcggc acattaatcg gttttatcat gcttggtttc gccaacagta tggtggtcct
      781 tttcatctcc cgcatcattg acggtctttc tggagcgaat atctctaccg ctcaagcagc
      841 gattgcagac agcaccaatg aaaagactcg cacgcaaggg ttgggtttgg tgggtgccgc
      901 attcggtgta gggtttgtgt tgggtcctat cttggctttc attgttttgg ctgcctcggg
      961 tcagaattat cagtacgtgg catggacagc cgcgcttttc tccctcattt ctattttgct
     1021 aacgacattc tggttcaaag aaacagttgc agatgccgcc aaccccgaag cctcgcgcaa
     1081 gcgccgtccg ttttcgtttg gtaatttacg cgaagctatg gcacaccgcc ccaccatcat
     1141 gttcctcgtg atcgtgatgt tcttctatca aatcgctttt ggcggatacg aacagctctt
     1201 ctcactcttc actctcaatc gcctcggcat ggatgcgcgt gacacctctg gcttgttcat
     1261 gttggcaggc atccttatca ttgtgatcca aggtggtttg atcggacgtt ggtctcgcaa
     1321 aaagggtgac cgttggctgg cattgatggg cattggtgcc ttagctatcg gcttgatcgg
     1381 cacggctctg actccagcca tgcctgtgcc ttggtacgat aaggcgcgtg tactcgaaag
     1441 ccttgccggg cagggtgagt tccgtgtttc tattcaaagc atcaacgtta gtttgccaga
     1501 tgaggcggct cgcggttggt tcggtattat ttggttgatg atcgccagca tccctgccgc
     1561 tttgggcggt gggacgctcc agccagccat caatagctta atcacaaaag attctgataa
     1621 gagcgaagtg ggtggaatgc tcgggttatc ggctgcggca tatagcgcgg caaatgcgat
     1681 tgcccccctg ttttatggtg cattattcca gtggtttggt gcgccagttc cattctttgc
     1741 gggcggagcg attttactgg cgctgttctt atttgcccca agagtgatta agaactcata
     1801 acgaaagaat gtgagcgcta gacgaaggtc tagcgctcac ggtttatagt cagaatctcc
     1861 aatagctttg ttgtatcttc tacaatctca tcggcgccaa gatgtcttag ctctggctct
     1921 tcgccaaaac cacatagcac gccaagggtt tgtgctccag ccgatttgcc cgcacggata
     1981 tcaaccgttg tatccccgat catgaggcag ttttccggcg agacgttcat cttctgcgcc
     2041 gcaagaagga tgggatcggg atagggtttg gtatgaaccg ctgaaagccc tgtaatcaca
     2101 gcatcgaaat atttcacaag gtcatactgc tcgagaaaac gcatggtccc tttttcatct
     2161 cgggcgctga cgacagccat ggggtaacgt ccgtgtagct gtttgagcat ctcatccacg
     2221 ccggggacga gtaggaattt tttatcagat tgtttgcggt gacgactcag ccaattgatc
     2281 acagccacca tttcatcatc caaatgaagc gtatcggcaa gaccaagcaa ggcattgccg
     2341 ggagcttcga tccacatcac gaagcgacgt gcggcatgtt ctgggtcctt aaaaagaaaa
     2401 cggggaaaca atttcgacac cttttgaacg tacaggtcat ctgtatcgct taaggtgcca
     2461 tctacatcga agcatagtgc atttatccta tatacgtcta gaggcatgga ttaattgtac
     2521 caaaggggct atacttaagc cgggataaga atgactgaag atcctaatcg tgccctcctc
     2581 gaattattag taaaggtcag ccgtgaggtt gccaccgcgc tggatctccg cacggtgttg
     2641 caacgggttc tgtacgcttg catccagtac gtgggcggtg agcgtggcag cattgtggtg
     2701 atggatgacc ttggcaaacc tgtagatgcc accattgtgt atggaaaaca attccatgat
     2761 cacaccacac aacaacttcg agaaacggtc gaacgtgggt tggcaggttg ggtggttcaa
     2821 aatcggaaac cagcccttgt ccctgatact agcaaggatg aacgttggct gcgccgcgca
     2881 gatgatgccg ccgaaaaaag tggagccaag tctgcgatct gtgtgccgct tctggcgcgc
     2941 gaacgctttg taggtgtgtt gacgctggtt caccctgttc ccaatgcttt taatgaacaa
     3001 catcttgaat tagtccaggc gatctccgat caggcgagca ttgcagtgtt gaatgcccgc
     3061 ttgtataccg agacccagcg cacggcgcga gttatgacgg cgctggcaga aggcgctgcg
     3121 acgatcaatt cttcgctgga aatgcaggat gtattacgtc gcatcttgaa tcagaccatg
     3181 caagccttgc aagtggaaac ggttgcgctg gggctgatcg actcgcatag caatgatctc
     3241 gtatttcaag ccgcagctgg aaataactct gggcatattc cggggttgcg tgaaccagca
     3301 ggaaaaggct tggtagggaa agtgattcaa gccgggcgcg gagtgattct tcctagcatc
     3361 aaacatgacc caacgttggt cgaagaagac aggctgaacg gagtcgagat gcgcgctctt
     3421 gctatcgctc cgattcaatt gcatggaaaa gtcattggcg ttttggaagc ggtgaatccc
     3481 atctctgggg catttgaccc ggatgcgttg gtggtgatga ccggcttggg gaatctcgca
     3541 ggaacgacca tcgataattc gaaattattc gagcaacttc aaaatgcaca caagcattat
     3601 cgcgagttat tcgaagacag cattgacccg atcctcgtta cggattggga aggcaaagtg
     3661 ttggaagcga accgtcaggc agtggagttg agcggctata ccaccgagca gcttcatact
     3721 atgagcattg accaactcca cgaagtgaat tggggcaagg tgggtctgag ctttgaaacg
     3781 atcaaagctg gcacctcttg tacgtatgaa tctgggatgt tacgccaaga tggcggcaga
     3841 ttgcccgtgg aagtatatgc acgccgagta gaatttgaag attctgactc cattcagtgg
     3901 atcttgagag acatcaccgc gcgcaaagat ttggatgcac tgcgcgatga tatgacctcc
     3961 atgatcttcc atgacttgcg ctcaccgctt ggcaatatcg tttcaagcct cgaaatgatg
     4021 agcacgctca tgccagaaga tgaaacgtta agctctatgc ttaacatcgc caagaattct
     4081 accgggcgca ttcagcgtct ggtcaattca ttgctagata tcaatcgtct cgaagcgggt
     4141 caacgcattg tggaccagaa ttcaatcgac ccggttgcgt tggtccgtga agccattcgc
     4201 gatgtggaac catcggtgac agcccgtcaa catgtgctta ccaaccgcgc attgagtgtc
     4261 ttgccgttga tctgggtgga cgtggatatg atccaccgcg tgttgatcaa cttgttggaa
     4321 aactccatca agttcacgcc cgctggcggg cgcattgaca ttggtgcaca gaccaatgaa
     4381 gacggtctgt tcgtcagatt ttggatacga gataacggtc ccggcattcc cgctgcggaa
     4441 cgtgaacgca ttttcgaaaa gtttacacgc ctgcgtggga agaacaaagc aggcggcttg
     4501 ggggtagggc ttgctttttg tcggcttgca gtccacgctc atgacggtga tatctggatc
     4561 gaaagtgaag aaggcaaagg aaccaccttc tggctcaccc tgcccgtggc gcaacgcaaa
     4621 tctgctggtc agatcaaacg ccagaccggt agactcacct tcaagccaga taagccataa
     4681 aaagatagaa agaacaacga aggacaatag atccgaaagg aatttttttc agaaccatca
     4741 tgcaggaaat cacaaagaac attttcatcg aagaccaatt tttaggcgtc accttgggcg
     4801 tgatcgtcac cccgcgcgga ttaattcaga tcgacgcgcc tccctctcct gaagatgccc
     4861 gctcttggcg cgcctctctc atgaatttag gcgggggcat cgaccgttta ctgatcaaca
     4921 tggatgccca tccagaccgc accctcggcg cccgcgccat ggattgcacc gttgtagccc
     4981 atgaaaagac tgcgtacatt ttccgtactc gtcccagcac tttcaaagca caaggcgaag
     5041 aaaccggcgc cgattgggaa tccattcccg gcttgggcag tgtgcgttgg gcacctcccg
     5101 agatttcttt tgttgaccaa atgaccctgc actggggcag ctcccctgtg atgttagaac
     5161 atcgccccgg tccttctggg ggctcgatct gggtcaactt gcctgaagag aaagttctct
     5221 ttgttggcga tatggtcatg aaaaatcagc cgcccttcct tgctggctct aacctcaaag
     5281 cctggctcga aagcttagac ctcttgctag agccggaata taaagggtat acatttatta
     5341 gcagccgcgg tggcgtggtc aatagcagtg ccatcaaagc acaatatgac ttcctaaagc
     5401 atgtccacga caaattgaat aagtccacta gcaagaagcc aaaccctgcc gctgtagaaa
     5461 aaatggttac gtcgttgttg acttggttca aagccccagc ggctcgtcag aaacaatttg
     5521 cccaacgttt gcgttacggc ttattgcact acaacgctcg gcagagctat gcacccagca
     5581 ataccataga agattaatga atacatctct ccctcttctt gaactcatgc gcggacctgt
     5641 ggttgaagcc acccaccttg gctccatcgc agtggtcaca gcagatggaa cgttgcttca
     5701 ctcttatggc gaccctcaca cggtcgcctt tttgcgctcc tctgccaaac cattccaagt
     5761 gctgcctttc gttgaagctg gcggcgtgga gcattacaac tacacccaac gtgagttggc
     5821 tctctcctgc gcttcacacg agacctcaca aatgcatctc gatgctgtga ccgctttaca
     5881 gaaaaaagtt ggcattgaag agcatgattt acagtgcggt ccacacctgc cgggtgatgc
     5941 tcgaaaatta cgcgaagtca ttcaacagaa catcctcccc actgcaaact tcaacaattg
     6001 ttcgggcaag cacacttcca tgttagcatt cgccaaaatg cgcggacttc ccctcacaaa
     6061 ctatcttgct atcgaccacc ccattcaaca agatattttg aagacacttt cagaaatgtg
     6121 caacatcgac gcgaatgaaa ttcaactcgg tgttgatgga tgctccgcac caaattttgc
     6181 actgccatta ttccatgcag ccctcggcat ggctcgcatg tgcgatacac gcgacctttc
     6241 cgaatctcgc gccactgcct gcaaaaaaat aaccaccgcc at
//
LOCUS       DAOQNJ010000086         6275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_7044_length_6275_cov_8.286174, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000086 DAOQNJ010000000
VERSION     DAOQNJ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7044_length_6275_cov_8.286174"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PLG52_03915"
     CDS             <1..1164
                     /locus_tag="PLG52_03915"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB40622.1"
                     /translation="IKINNRKLKKVSMKKNTLLFITLIVLLTLVLSACGGGQQTAATQ
                     EPVANVINASDVVAEGRLEPAHATNLTFQARGVVEDVLVKVGDTVSEGDVLARLANAG
                     AAEAQVVVAQNAYDTLLRNESGDRAKLWEVYMNAQIVRAEAEKKWEDVDEDAVKNRIE
                     DINAEIEDLKDTLKDAQDEFDKYKDLDEDNSKRKAADDDLKTAQDNLNAKIRELEEET
                     RSRDEVKAAYEAALDAEAEAKYQYEISLDGPNADQLTLAKANLDAAKDALSNYVITAP
                     FSGVVADVNVKAGEQVGTDTRVVSIIDDSQWFVKTTDVTELEVVSLSIGQAVTIRPDA
                     LTDLEMTGTVTEISSAYTQQGGDILYTVRVRVDGSDPRLKWGMTVETVFRTGE"
     regulatory      1202..1288
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      1368..1462
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1563..2588
                     /gene="ruvB"
                     /locus_tag="PLG52_03920"
     CDS             1563..2588
                     /gene="ruvB"
                     /locus_tag="PLG52_03920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNB40623.1"
                     /translation="MAKTPSKALDPEAKPDDRVDNALRPQKLNDLIGQNQVKENLSIL
                     IAAARQRSEALDHVLFYGPPGLGKTTLAHVLGNEMGVNVKVTAGPAIERAGDLAAILT
                     NLRAGDILFIDEVHRLGRAVEEVLYPAMEDFALDIVIGKGPSARSIRLKLPRFTVVGA
                     TTRLALVTAPLRARFGAVYRLDYYDLDAMKQIVGRAAGLLKVQADESGIAEVARRARG
                     TPRIALRLLRRVRDFAQVRADGVITQNVAKEALDLLQVDPLGLDDVDRRVLKTIIEKY
                     NGGPVGLNTIAASISEEQDTIMDVVEPYLLQLGFLDRTPQGRVATKSAYEHLGYQYTE
                     EGQPRLL"
     gene            2600..2890
                     /locus_tag="PLG52_03925"
     CDS             2600..2890
                     /locus_tag="PLG52_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40624.1"
                     /translation="MKQEVIIKGNANEDLTRLIEVAIKNQLEILEKDIRASKVRLKAF
                     EKKAGVSSEKFETMLASGELNECLDTLDWKMELASFRLLNQKYKSLIEAEVS"
     gene            2895..3122
                     /locus_tag="PLG52_03930"
     CDS             2895..3122
                     /locus_tag="PLG52_03930"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNB40625.1"
                     /translation="MESIARYLMLGGIILFCIGGGVYLASRFGIPLGRLPGDIRIEGE
                     HGSFYFPVTSSILVSVLLTIVLNLITRFLNK"
     gene            3144..4034
                     /locus_tag="PLG52_03935"
     CDS             3144..4034
                     /locus_tag="PLG52_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HNB40626.1"
                     /translation="MKSKWTPLIIGILFMFFCCIPLVIGAFFFQTRPPGALSDSGYYV
                     RLTKVYYYPGFGISQAFVIQGADPFSFETIDSTYAKDKAHVYYDGVQIPDADPATFEL
                     LDSTYARDSRHVYLNGQIFSNDPANFENVSENIYRDSQYIYWSGEPISEDPQNLVVLG
                     NFGFYTYLKDSQTVFVNGNSIHGANPNTFQVISEGYSRDSEHVFYFDELISTADAATF
                     EALEQQYARDAVSAYWMGKPIPNSDPASFDILNADFECTADANHAYYQDQLIEGFDPA
                     TIPTNAQVNSCDLTTVYFTP"
     gene            4031..5071
                     /gene="queA"
                     /locus_tag="PLG52_03940"
     CDS             4031..5071
                     /gene="queA"
                     /locus_tag="PLG52_03940"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNB40627.1"
                     /translation="MKTNDFDYHLPESSIAQTPAEPRDSSRLLVLHRTTGDVEHRLFR
                     DVSLFLRPGDLLVLNQTRVIPARIFAHKETGGKVELLLLRRRDALTWEVLVGGKGLRI
                     GKIVQVENGPSAEIIELLEGSERLIKFSEPIEPYFSQVGNVPLPPYIHEKLNDPERYQ
                     TVFSREPGSAAAPTAGLHFTPELLAKIQKMGVKTAYVTLHVGLDTFAPVNEDDPEEHK
                     IHSEWCHLSQETADAINETKRNGGRVIAVGTTSVRTLESAGKDLILDVRSPNTEYRIS
                     PFTGPTTLYILPGYRFKVVDAMITNFHLPKSTLLMLVSAFAGREKILETYNLAIREGY
                     RFYSFGDAMFIE"
     gene            5094..5759
                     /locus_tag="PLG52_03945"
     CDS             5094..5759
                     /locus_tag="PLG52_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNB40628.1"
                     /translation="MNLEQHNKQIIACRKCPRLVEWREEVAQVKRKAYINEEYWGKPV
                     PGFGDPKARVLVVGLAPGAHGSNRTGRQFTGDASGNFLYPALHKAGFANQPTSESRSD
                     GLALKDMYITASGRCAPPDNKPSPEELNNCQPFLERELEIINPKVIVCLGRIAFERIL
                     KIYSEKSSVYKFAHGASYQLANGKWLLCSYHPSQQNTSTKKLTPTMFDAIWVKAKQLA
                     DRN"
     gene            5765..>6275
                     /locus_tag="PLG52_03950"
     CDS             5765..>6275
                     /locus_tag="PLG52_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB40629.1"
                     /translation="MKKINWKIVAAVITAVLLVLVAGFVFWASDASAATDVALQALNS
                     DSKVNVSAENGWVIFTPAENSRPETGFIFYPGGKVDYRAYAPILKMISAKGYFVAVVP
                     VPLNLAFFDINAASRVEAAYPEVKNWFVGGHSLGGVAASSYAASHEEIRGVVFWASYP
                     ADDSLKTKNI"
BASE COUNT         1680 a         1404 c         1572 g         1619 t
ORIGIN      
        1 ataaaaatca acaatcgaaa attgaagaag gtttccatga agaaaaatac actacttttt
       61 ataactttga tcgtgctcct tacgcttgtt ctctcagcgt gcggcggcgg tcaacagacg
      121 gctgcaactc aagaacctgt tgccaatgta attaatgcca gcgatgttgt tgctgaagga
      181 cggcttgaac ccgctcatgc cacgaatttg actttccagg cacgcggcgt tgtggaagat
      241 gtgcttgtca aagttgggga tactgtcagc gagggagatg ttttggctcg cctcgcaaat
      301 gcgggtgcgg cagaggcgca ggttgtcgtc gcacaaaatg cttacgatac gttgctccgt
      361 aacgagagtg gtgaccgtgc caagttgtgg gaagtatata tgaatgccca aatcgttcgc
      421 gcagaagcgg aaaagaaatg ggaagatgta gatgaagatg ccgttaagaa ccgtattgaa
      481 gatatcaatg cagagatcga agacttaaag gatacgttga aagacgcaca agacgaattc
      541 gataagtaca aggacctcga cgaagataat agcaagcgta aggctgctga tgacgacctc
      601 aaaacagcac aagataacct caatgcgaag attcgtgagt tagaagaaga gacccgttca
      661 cgcgacgaag tgaaagcggc ttatgaggct gctcttgatg cagaagcaga agctaaatat
      721 caatatgaga ttagcttgga tggtcccaat gctgatcagc taacacttgc caaggcaaac
      781 cttgatgcgg cgaaagatgc cctttctaac tatgtcatca cagctccatt ctctggcgtt
      841 gtagcagatg tgaacgtcaa ggctggcgaa caggttggca cagatactcg tgtggtaagt
      901 atcattgatg atagccaatg gtttgtgaag accacggatg tgactgaatt ggaagttgta
      961 agtttgagca ttggtcaggc agttaccatt cgtcctgatg ctctgaccga ccttgaaatg
     1021 actggtactg ttaccgagat tagcagtgct tacactcaac aaggcggcga tattctttac
     1081 accgtccgcg ttcgtgtgga tggttcagat ccacgcttga aatggggtat gacggtcgaa
     1141 accgttttta gaacaggtga gtgattgaaa ttgcacgaaa agagtgcaat aataaaattg
     1201 ctccgagaaa gagcaaaacc gggaaaaccc ggcggcgcaa agccctactc taaagctgaa
     1261 ctgagccatg agtgtgaggg ttgccgataa aagcaaaacc gttaaaagac ggcggcgcaa
     1321 agtcagagcg tctacctctg gttactgtta ccggataaga ccgaccgact gccgaaaggc
     1381 aaacccgggg aaacccggcg acgcaaagtc atgggtctac aatcgtgatt tgatagacga
     1441 tcaagatcgc cagactgccg aagagcaaac cgtttgcagg gatgacattt taggtcatcc
     1501 ctgcttggtt ttaagctaat tgtttgcagg gcaagctacc tgtaaagggt ataatcgcgc
     1561 acatggcaaa aacaccttcc aaagcactcg accctgaagc caagcccgat gaccgcgtgg
     1621 ataacgcatt gcgcccacaa aagctcaatg acctgattgg acagaatcag gtaaaagaga
     1681 atttatccat tctcattgcg gcggcgcgtc aacgtagtga agcgttggat catgtcttgt
     1741 tttacggtcc ccccggtttg ggaaagacta cacttgcaca cgtgcttggg aacgagatgg
     1801 gggtcaatgt caaagtcacg gcaggacccg ccattgagcg tgctggcgat ttggctgcca
     1861 ttctcacgaa tttacgtgct ggcgatattc tctttattga tgaagtccat cgccttgggc
     1921 gggctgtgga agaagttctc tacccagcta tggaagattt tgctttggat attgtcattg
     1981 gcaagggacc ttcggctcgt tccatccgcc tgaaactgcc tcggtttacg gtagtggggg
     2041 ctacgacccg gctggctctc gtcacggcgc cgttacgcgc gagatttggc gcggtctatc
     2101 gtttggacta ttacgatttg gatgccatga agcagattgt tggacgggca gcagggttgt
     2161 taaaagttca ggcagatgag tcaggcatcg cggaggttgc gaggcgcgca cgcggtacgc
     2221 cgcgtattgc tttgcggttg ctgcgtcgtg tgcgagactt tgcccaagtc cgcgcggatg
     2281 gtgtcatcac ccaaaacgtt gcaaaagaag cattagactt gttgcaagta gacccgcttg
     2341 gtttggacga tgttgaccgc cgcgtgctca agaccattat cgaaaaatac aatggcggac
     2401 ccgtgggttt gaataccatt gctgcatcca ttagtgaaga acaagatacg atcatggatg
     2461 tggttgagcc ctacttgttg caacttggct ttttagaccg cactccacaa ggccgtgtag
     2521 cgaccaaatc tgcatatgag catttggggt atcagtatac agaagagggg caacccagat
     2581 tgttgtaagg atcatcacca tgaaacaaga agtcattatc aaaggcaatg caaatgaaga
     2641 tttgacccgc ttaatagaag tggcaatcaa aaatcaatta gagatccttg aaaaagatat
     2701 ccgcgcctcc aaggtccgct tgaaggcgtt cgaaaaaaaa gctggggtat cttcagaaaa
     2761 atttgaaacc atgctggcaa gcggggagtt gaatgaatgt ttggatacat tggattggaa
     2821 aatggaattg gcatctttcc gcttattgaa tcaaaaatat aagagcctga tcgaagcaga
     2881 ggtatcctaa tcaagtggaa agtattgccc gttacctcat gctcggcgga atcattctct
     2941 tctgcattgg cggaggggtc tatctagcgt cccggtttgg aattccgctt gggcgcttgc
     3001 ccggcgatat ccgcattgaa ggcgagcatg gctcgtttta ttttccagtg acaagttcga
     3061 tcttggtttc agtactactt accattgttt taaatctcat tacccgtttt ctgaataaat
     3121 aactttatcc ataaggtgta cctatgaagt caaaatggac tccgttgatt atcggtattc
     3181 tttttatgtt tttttgttgt atcccacttg taattggggc tttctttttt caaacccgcc
     3241 cgccgggagc cttaagcgat agcggatatt acgtccgcct gacaaaggtc tattactacc
     3301 ccggttttgg gatttcgcag gcatttgtca ttcaaggtgc agacccattc tcttttgaaa
     3361 caattgattc gacttatgca aaagataagg ctcatgtgta ctacgacggc gttcaaattc
     3421 cagatgcaga cccggcaaca tttgagctgc tggattcaac ctacgcaaga gattctcgcc
     3481 atgtctattt gaacggtcag atatttagca acgaccctgc aaatttcgaa aatgtatcag
     3541 aaaatattta tcgtgatagc cagtacattt actggagcgg cgagcctatt tctgaagacc
     3601 cacagaattt ggtagtgctc ggcaactttg gcttttatac ctacctgaaa gattcgcaaa
     3661 ctgtttttgt caatggcaac tccatccatg gcgcaaaccc aaatacattt caagtgatta
     3721 gtgagggata ttcacgtgac tcggaacatg tattttattt cgacgaacta atttcaacag
     3781 ccgatgccgc cacattcgaa gcgctggaac aacaatatgc gcgcgatgcc gttagcgctt
     3841 attggatggg caaaccaatt cccaattcag accctgcatc ttttgatatt ctcaatgcag
     3901 attttgaatg taccgccgat gcaaaccacg cctattatca agaccaattg atcgaaggct
     3961 ttgaccctgc caccattcca accaatgcgc aagtcaacag ttgcgacctg actactgtat
     4021 acttcacgcc atgaagacca acgactttga ctaccatctt cctgaatcgt ccattgctca
     4081 aacgcctgcc gagccgcgtg attcatctcg actccttgtt ctacatcgca ccacagggga
     4141 tgtagaacac cgcctctttc gtgacgtaag cctgtttcta cgcccaggcg atttactcgt
     4201 cctgaaccag acgcgcgtga ttccagcccg catttttgcc cataaagaga ccggcggcaa
     4261 agtggagttg ctccttctcc gccgccgaga tgcgctgact tgggaagttt tagtgggagg
     4321 caagggtttg cgcataggca aaattgtgca agtagagaat ggaccaagtg ctgagattat
     4381 cgaattgctc gaaggttctg aacgcctcat caaattttct gaacccattg aaccgtattt
     4441 ttcacaggtg gggaatgtgc ccctcccacc gtacatccac gaaaaactaa atgacccaga
     4501 acgctatcaa actgtctttt cgagagagcc ggggtctgcc gctgctccca cagcagggtt
     4561 acactttacg cctgaactgc tcgcaaaaat tcaaaagatg ggtgtaaaga ccgcctacgt
     4621 tactttgcac gttggcttag atacctttgc gccagtcaat gaagatgacc ccgaagaaca
     4681 caaaatacat tctgaatggt gtcacctttc gcaagagacg gcagacgcca tcaacgaaac
     4741 caaacgcaat ggaggaagag tcatcgcggt tggcacaaca tcggtgcgga cgttggaaag
     4801 cgccggaaaa gacttaatac ttgatgttcg ctcaccaaat accgaatatc gaatatcgcc
     4861 atttacagga cctactactt tgtacatcct ccctggctat cgatttaaag tggttgatgc
     4921 catgatcacg aattttcacc tgcctaaatc cacgctctta atgttggtca gtgcctttgc
     4981 tgggcgcgag aaaattcttg aaacgtataa tctcgcgatt cgagaaggct atcgcttcta
     5041 ttctttcggt gatgcaatgt tcatcgaata accttatatt tctacttatt cctatgaacc
     5101 tagaacaaca taacaaacaa atcattgctt gtagaaaatg tccgcgtttg gtggagtggc
     5161 gcgaggaagt ggcgcaagtc aaacgcaagg catatatcaa cgaggagtat tggggaaaac
     5221 cagtgccagg ttttggcgac ccgaaggcac gtgtgctcgt ggttgggctt gctcctgggg
     5281 cgcatggttc gaaccgtaca ggtcgtcagt tcacgggtga tgcttcgggg aattttttgt
     5341 atccggcgtt gcacaaggct gggtttgcga accagcccac gtctgagtct cgaagcgatg
     5401 gacttgctct aaaggatatg tatatcaccg cgtcgggtcg ttgcgctccg ccagataata
     5461 agccgagtcc agaagagcta aataattgtc agccttttct tgaacgtgaa ttagaaatta
     5521 ttaatccgaa agtcattgtt tgcctgggtc ggatagcttt tgagaggatt ctaaaaatat
     5581 attccgaaaa aagttcggta tataaattcg ctcacggcgc gtcttatcaa ctggcaaatg
     5641 ggaagtggct gctttgttca taccacccca gccagcaaaa tacctctaca aaaaaactga
     5701 ccccaacgat gttcgatgcg atttgggtga aggcaaaaca actcgcagat aggaattgat
     5761 gtgcatgaaa aaaataaatt ggaagatcgt agcggcggtg atcaccgccg ttttgttggt
     5821 acttgttgcc ggttttgtgt tttgggcaag tgatgcttca gccgccactg atgtggcttt
     5881 gcaagcattg aactcagatt cgaaagtgaa tgtttcggct gagaatggct gggtgatttt
     5941 taccccggca gaaaattcac gacctgaaac gggctttatt ttttaccctg gtggtaaagt
     6001 ggattatcgc gcatatgcgc cgatcttgaa aatgatttct gccaaggggt atttcgtagc
     6061 ggtagttccg gttccattga atttggcttt ctttgatatc aatgcagctt ctcgggtaga
     6121 agcggcgtat cctgaagtca agaattggtt tgtaggtgga cactcgttag gcggcgtggc
     6181 ggcatcttct tatgcggcaa gtcacgaaga aattcgaggt gtggtgtttt gggcttcgta
     6241 tccggctgat gattctttga agaccaagaa catcc
//
LOCUS       DAOQNJ010000087         6151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_7278_length_6151_cov_10.191601, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000087 DAOQNJ010000000
VERSION     DAOQNJ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7278_length_6151_cov_10.191601"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1448
                     /gene="obgE"
                     /locus_tag="PLG52_03955"
     CDS             183..1448
                     /gene="obgE"
                     /locus_tag="PLG52_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNB40630.1"
                     /translation="MFIDQVNIHVKSGKGGDGMVHFRREKYEPRGGPDGGDGGKGGDV
                     IFEVKPILNSLSHFKPKQSFAAENGINGGGSQMTGRGGKDLTIHVPPGTVIYDAETGA
                     LLGDLTDPGQQLIVCKGGRGGRGNQHFATSRNQAPRTAERGEPHEEKMLRLELKLIAD
                     IGIIGLPNAGKSTLLSVLTNAKPKIGDYPFTTLEPNLGVAKIDEEATVVLADIPGLIE
                     GAHEGAGLGHDFLRHIQRTRVLIHMIDGLSEDPLADFSQINTELSLFDTKLGKKPQVV
                     VINKIDQPDVQARLKEIQTSFKKKKVEIITASAMARNNTRDVLLTAYRMLQELPPEQL
                     GEEALPVYKPEVDPNLFDIHREDGDKWRVSGVAIERAAKMTYWEHFGSVRRFQRLMQK
                     LGVDKALREAGVQDGDTVFVGDFELEWQE"
     gene            1539..1967
                     /locus_tag="PLG52_03960"
     CDS             1539..1967
                     /locus_tag="PLG52_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40631.1"
                     /translation="MTKQRILTTGLIVIGLVVVIFFGMNTLRAFKHMRGHGPFNGKPP
                     TANQTDVELIRDWMTLPYIGSMYDVPPEALFYSLGIEPGKQNGKKSLKELNDEFFPEQ
                     PDLAISQVKDTIKAFQTQERPPEPFPPVAPTPPSPPTQNP"
     gene            1964..2575
                     /locus_tag="PLG52_03965"
     CDS             1964..2575
                     /locus_tag="PLG52_03965"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNB40632.1"
                     /translation="MTDTISGFFLTQVINYGAPLFALTLFLGAVGLPVGASLMVIAVG
                     AFSQQGYFEWTTMALLGLLGAALGDALSFGMGHYGKGWVEKRFGTSPTWKNAQSYFDK
                     RAWVAIFLTRFLVTALAIPTNLIAGGSGYKFQRFMLYDVLGEMTWIILYGGLGYAFGS
                     QWEVVYDFIGNFGGFVLGAVILGAGIWMARNQFNKPKQEQLAV"
     gene            complement(2609..3100)
                     /locus_tag="PLG52_03970"
     CDS             complement(2609..3100)
                     /locus_tag="PLG52_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB40633.1"
                     /translation="MANDTVKDGLVVSMEYKLTVDGEVLDSSDEAGPLQFLAGYGNII
                     PGLENEMMGMKIGDSKNVTVQPADGYGEFEDDAFVDVPRTEFPADMEIEEGMELHVTD
                     EDGNHQAAYVAEFDDKTVKLDFNHPLAGAVLDFYVKVVALREPTDEELDHGHVHEGDG
                     HHH"
     assembly_gap    3347..3446
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3447..4142
                     /locus_tag="PLG52_03975"
     CDS             <3447..4142
                     /locus_tag="PLG52_03975"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNB40634.1"
                     /translation="ETADAWLNNMNGFFVQEHHVLEAINSAKSGEIDEGNVGGGTGML
                     CYEFKGGTGTSSRKLPEKLGGWTVGAIAQCNFGRRYQLTIAGVPIGHHLTDDAPATNG
                     ENPFKQDDGSLIVIIATDAPLLPHQLKRVAKRVSLGMSRTGAIGGNGSGDIFLAFSTA
                     NSEAAKGLGRTQLEALPNGQLDPIFAATAYATEEAIVNALVAAEDMTGHEISAKALPH
                     EDVKELLKKHGRL"
     gene            4291..4845
                     /locus_tag="PLG52_03980"
     CDS             4291..4845
                     /locus_tag="PLG52_03980"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNB40635.1"
                     /translation="MLKYALLGFLSYSPLTGYELKQRMDSSTNNFWNANLSQIYTSLK
                     SLEEDGWVKSKIQAQDDKPDKRIYTLTDEGLSSLKAWLEEPITDPEQRKSTFLLKLFF
                     SAKVDKETSLAQLRVSKNLHQREKERYKSEAQTSIKDIVATYPSMKKEAIFWEATRRF
                     GELYEEMYVHWLSETIELIENKMK"
     gene            4862..5806
                     /locus_tag="PLG52_03985"
     CDS             4862..5806
                     /locus_tag="PLG52_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB40636.1"
                     /translation="MASILEVQNLEKNYGDFKAVKGISFDIKEGEIFSLLGPNGAGKT
                     TTISMLSTLYTPTAGDATIAGHSITKAPMAVRNAIGVVPQDLALYEDLNARENLTFWG
                     QMYGLGGSALKNRVEEVLNQIGLVDKAKDRVKTYSGGMKRRVNIGVGLLHKPKLLFMD
                     EPTVGIDPQSRRAILDTVKDLNKQGMTVLYTTHYMEEAEELSNRVGIIDHGELIAIGT
                     QKELTQQVGETETLILHISENEDPEALVTAFKGIKGVLEAKSVDHEISVITPSAADVM
                     APVVTKANERGIKIHSIDIREPNLEAVFLHLTGRALRD"
     gene            5811..>6151
                     /locus_tag="PLG52_03990"
     CDS             5811..>6151
                     /locus_tag="PLG52_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40637.1"
                     /translation="MLKTFIIGIKDVKLAFRDRAALIFMLLAPFLLTLGMGLVTGRFS
                     GDNSSGISDIPVVIVNQDDGQLSKALVDLFYSEDLANLVEPSLGTSPEAARQMVDDDE
                     VATAIIIPAGFT"
BASE COUNT         1695 a         1442 c         1429 g         1485 t
ORIGIN      
        1 agggtgctgc ggccttgttt gtatactact gaacgtatac aaacaagagc ttatttttat
       61 cctagcatca ttctacatct caattaatag ccatttacct tatctatcat gagtctattc
      121 aagcaaatac cccttaattt gtattccctc gaaagatcac ttatagccat tataatgggg
      181 gaatgttcat tgaccaagta aatattcatg taaaatctgg aaaaggcggc gatggtatgg
      241 tgcattttcg ccgtgaaaaa tacgaaccta ggggcggtcc ggacggcgga gacgggggca
      301 aaggcggcga tgtcatcttt gaagtgaaac ccattcttaa ttccctctct cactttaaac
      361 ccaagcaatc ttttgcagcc gaaaatggca tcaacggcgg cggttcgcaa atgaccgggc
      421 gtggcggcaa agacctgaca attcatgttc caccgggaac cgtcatctat gatgcggaaa
      481 ccggagcatt gctcggcgac ctcacagacc caggacagca actcatcgtc tgcaagggcg
      541 ggcggggtgg acgtggcaac cagcattttg ccacctcacg aaaccaagcc ccaaggaccg
      601 ccgaacgcgg cgaacctcat gaggaaaaaa tgctgcggct cgaacttaag ctgattgctg
      661 atattggcat cattggctta cccaacgccg ggaaatccac tctcctctcc gtgctgacga
      721 acgccaaacc caagatcggt gactatccct tcacaactct cgaacccaac ctgggtgttg
      781 ccaaaattga tgaagaagcc accgttgtgc tggcagatat ccctggtttg atcgaaggcg
      841 ctcatgaagg tgcagggttg gggcacgact ttttacgcca tattcaacgc actcgcgtcc
      901 tcattcatat gattgacggg ttatcagaag acccactcgc cgatttcagt cagatcaata
      961 ctgagctttc gctctttgat acaaagctag gcaaaaagcc gcaggttgtg gttatcaata
     1021 agattgacca gcctgatgtg caagcgcgat taaaagaaat tcaaaccagc ttcaaaaaga
     1081 aaaaagtgga aatcatcact gcctcagcca tggcacgcaa caacacacga gatgtgttgc
     1141 tcacagcgta tcgtatgctt caggaactgc caccagaaca actcggggaa gaggcgttgc
     1201 ccgtctacaa accggaagta gacccgaact tgttcgacat ccatagagaa gatggagata
     1261 agtggagagt atcgggggta gcgattgaac gtgctgcaaa gatgacgtat tgggaacact
     1321 ttggctctgt gcggcgcttc caacgtttga tgcaaaaact cggcgtggat aaggcattac
     1381 gcgaagccgg ggttcaagat ggcgatacgg ttttcgtagg cgattttgaa ttggaatggc
     1441 aagagtaacg ataaaaggac acaccacatg gtgtgtcctt tttgattcca cattatttct
     1501 ttacaatttg aacctgaaag cagggataaa atacgcatat gactaaacaa cgcattttaa
     1561 caaccgggtt gatcgtcata ggattggttg tcgttatctt ttttggcatg aatactcttc
     1621 gcgcctttaa acacatgcgc ggacatggtc cattcaatgg caaaccacct acagcgaatc
     1681 aaactgatgt ggaattgatc cgagattgga tgacgcttcc ttacattggc agtatgtatg
     1741 atgttccgcc agaagccttg ttctacagtt tagggattga acccggaaaa caaaacggga
     1801 agaaaagttt gaaagaattg aacgatgaat tcttccctga acaaccagac ctggcgatct
     1861 cgcaagtaaa agatacgatc aaggcattcc aaacacagga acgtcctccg gaaccattcc
     1921 cacctgttgc accgactcca ccgtcaccgc cgactcaaaa cccatgaccg atacgatctc
     1981 tggatttttc cttacacaag ttattaacta cggggcgcct ctttttgcgt tgacgctttt
     2041 tcttggagcg gttgggctac ccgtaggtgc ctctctgatg gttattgctg ttggcgcttt
     2101 ctctcaacaa ggatatttcg aatggaccac gatggcactt ctaggcctgc tcggggctgc
     2161 actaggagat gctctcagct ttggaatggg tcattatgga aaaggctggg ttgaaaagcg
     2221 ctttggcacc tcccccactt ggaagaacgc tcaatcctat tttgataaac gtgcctgggt
     2281 agccattttt ctcactcgct ttttggtaac ggcgttggca attcccacaa acctgattgc
     2341 tggtggcagc ggatataagt ttcaacgttt tatgctatat gatgttctcg gcgaaatgac
     2401 ctggatcatt ctgtatggcg ggttgggata tgcatttggg tctcaatggg aagtggtcta
     2461 tgattttatt ggaaactttg gtgggtttgt attaggtgcc gtgatcttag gtgctggcat
     2521 ttggatggca agaaatcaat ttaacaaacc caaacaagaa caactagctg tataaaaaaa
     2581 catcctctat acagaggatg tttttttatt agtgatgatg tccatcgccc tcgtggacat
     2641 gaccgtgatc gagttcttcg tcagttggct cacggagagc tacaactttg acatagaaat
     2701 ccaacacagc gccagcaagg ggatgattga aatcgagctt gacggtcttg tcatcgaatt
     2761 cagccacata cgcagcctga tgattaccat cttcatcggt aacgtgcaat tccatacctt
     2821 cttctatttc catatcagca gggaattcag tgcgtggaac atcaacgaaa gcatcgtctt
     2881 cgaactcgcc atatccatcg gctggttgaa ctgtgacatt tttgctgtcg ccgatcttca
     2941 ttcccatcat ttcattttcc aaaccgggaa tgatattgcc gtaccccgcc agaaactgca
     3001 aaggacccgc ttcgtcagaa gagtccaaaa cttcgccgtc tacggtcagt ttgtattcca
     3061 tggaaaccac caaaccgtct tttactgtat cgtttgccat acttgtatcc tttcgaattt
     3121 aatgttccca ttgtaccagc aataaataga ctgcaaggca tccacagaac cccttcggct
     3181 ataatgttgg cgtcttttca atgctttgcc aaggagaatg tatgaaaaaa atacgtgccc
     3241 gcgatctggg catccctttt gagggaacac ctggggcatt caacgcaatc acagacgtgc
     3301 ctggtattac ggttggatac tccactatta tcgaagggac agatgcnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnngaaa ccgcagatgc ctggctcaat aacatgaatg
     3481 gttttttcgt acaagaacac catgttcttg aggcgatcaa ctctgccaaa tcgggtgaga
     3541 tcgatgaagg caacgtaggc ggtgggacgg gtatgctatg ctacgaattc aagggcggaa
     3601 cgggaacatc atccagaaaa ctgcccgaaa aacttggcgg ttggacagtc ggcgcgatag
     3661 cgcaatgtaa ctttggcaga cgttatcaac ttacgattgc aggcgtacct attgggcatc
     3721 acctaacaga tgacgcccct gccaccaatg gtgaaaatcc gtttaagcag gatgatggtt
     3781 cattgatcgt catcattgcg accgacgccc cacttttacc ccatcaactg aaacgtgtcg
     3841 caaagcgggt ctctcttgga atgagtcgta cgggcgcaat tggcggaaat ggctctggtg
     3901 atattttcct tgcattttcc acagcgaatt ctgaagcggc aaagggactg ggacggaccc
     3961 aacttgaggc attgccgaac ggtcaacttg acccgatctt tgccgccaca gcttatgcaa
     4021 cagaagaagc gatcgtcaac gcgcttgttg cagctgaaga tatgacaggg catgagatca
     4081 gcgccaaggc tttgccacat gaagatgtga aagaactctt gaagaaacac ggcaggttat
     4141 aaatcagtaa atccgcttaa ctgactatac gaaaattcta taaaccctag tcgtttatga
     4201 ctagggtttg tagtttaaga gcccttgaca aaacaaaacc tataatatat aaacagttat
     4261 atataaaaat ttatatataa aggatgcctt atgctcaaat atgccttact cgggtttctc
     4321 agctattccc ctttaacggg gtacgagcta aaacagcgta tggatagttc tacaaacaat
     4381 ttctggaatg cgaaccttag tcagatctac acttcgttga agtctctaga ggaagatggc
     4441 tgggttaaat caaagattca ggctcaagac gataaaccgg ataagcgcat ctacacgttg
     4501 accgatgaag ggcttagcag tctaaaggcg tggctcgagg agcctattac cgaccctgag
     4561 cagagaaaaa gcacattctt gctaaaactt ttcttctcgg caaaggtgga taaagaaaca
     4621 tcccttgccc agcttagggt atccaaaaac ctgcaccagc gcgaaaaaga acgatacaaa
     4681 tctgaagcac aaacaagtat taaagacatc gtcgcaacct acccaagtat gaaaaaagaa
     4741 gccattttct gggaagccac gcgaagattt ggagaacttt acgaagagat gtacgtacat
     4801 tggttatctg aaacgatcga acttattgaa aacaaaatga agtgaacaca aaggaacaat
     4861 catggcatct atactggaag tacaaaacct cgaaaaaaac tacggtgact ttaaagctgt
     4921 aaaagggatt tcgttcgata tcaaagaagg cgaaatcttc agcttactcg gaccgaacgg
     4981 cgccgggaag accaccacca tctcaatgct ttccacactc tacaccccca ccgctggcga
     5041 tgcgaccatt gcgggtcact ccattaccaa agcccccatg gcggtgcgga atgccattgg
     5101 tgtcgtaccg caagaccttg ccctatacga agacctcaat gctcgcgaaa acctcacttt
     5161 ttgggggcaa atgtatgggc taggcggctc ggctcttaag aaccgcgtgg aagaagtgtt
     5221 gaatcagatc gggctggtgg ataaagccaa ggatcgtgtc aaaacgtatt cgggcgggat
     5281 gaagcgccgc gtcaacattg gggtgggctt gctccacaaa ccaaagcttc tcttcatgga
     5341 cgagccaacg gtgggtatcg acccacaatc tcgccgtgcc attctcgaca cggtaaaaga
     5401 cttgaacaaa caaggcatga ccgtcctcta caccactcat tacatggaag aagccgaaga
     5461 actctccaac cgcgtgggca ttattgacca tggtgagttg atcgccattg gtactcaaaa
     5521 agaactcacc caacaagttg gcgaaaccga aacgttgatt ctgcacatta gcgagaatga
     5581 agaccccgaa gctttagtga cggcattcaa aggcattaag ggcgtgctcg aagccaaatc
     5641 ggttgaccat gaaatttccg tcatcacacc ctctgcagca gacgttatgg ctccggttgt
     5701 gaccaaagcc aatgaacgcg gaatcaagat ccactcgatt gatattcgcg aaccgaattt
     5761 ggaagccgtc ttcttacatc tcacagggcg cgcactgcgc gattaacatc atgctcaaaa
     5821 catttattat cggcatcaaa gatgtaaaac tcgcctttcg cgaccgtgcg gcattgatct
     5881 tcatgctgtt agcgcccttc ctactcacct tgggcatggg gctggtcacc gggcgtttta
     5941 gcggagacaa ttcatctggc atttcagata ttccagtggt gattgtcaat caggatgatg
     6001 gacagctgag caaggcgttg gtggacctgt tctacagcga agacttggct aacttggttg
     6061 agccaagcct cggcacgagt cctgaggcgg cacgtcagat ggtggacgat gacgaagttg
     6121 ctactgccat catcatccct gccgggttta c
//
LOCUS       DAOQNJ010000088         6114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_7339_length_6114_cov_9.837432, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000088 DAOQNJ010000000
VERSION     DAOQNJ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7339_length_6114_cov_9.837432"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLG52_03995"
     CDS             complement(<1..182)
                     /locus_tag="PLG52_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40638.1"
                     /translation="MIRVAKTEDAVKIAEIYNHYIANTIITFEKEPVTPDEISTRMTD
                     VFSSSLPWLVLENNDEV"
     gene            complement(207..716)
                     /locus_tag="PLG52_04000"
     CDS             complement(207..716)
                     /locus_tag="PLG52_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40639.1"
                     /translation="MPKQRDSRFITIRRGGTLQDSDHHLLALWAADCAEHVLYLFENV
                     CPQDDRPRQAIESARAWTRGEISMSQSRAAGGHAMAAARALSGAARHAAFAAGQAGAV
                     AHVAAHELGAAAYAIKAVQAAALEGESKEAGRLECQWQRAQLPSQIRALVLDDQKLRN
                     EICWFVFDC"
     gene            complement(744..1025)
                     /locus_tag="PLG52_04005"
     CDS             complement(744..1025)
                     /locus_tag="PLG52_04005"
                     /inference="COORDINATES: protein motif:HMM:NF025482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HNB40640.1"
                     /translation="MSFQAYLDKVEELTGKAPKEFVAEAKKKKLTTHKEILDWLKTDY
                     GLGTGHARAIAHVILHGAEFTVKQTTGTHRDASGTLNLNGKKAAKKKKS"
     gene            complement(1052..2731)
                     /locus_tag="PLG52_04010"
     CDS             complement(1052..2731)
                     /locus_tag="PLG52_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNB40641.1"
                     /translation="MANPEHLEALKQGMEKWNTLRSKNFDTVFDLSGADLSGMNLVGI
                     RLYNVNLSGANLSNAYLRNADFSSSDLSGANLTGADLSQSYMFGVKLSDANLEGAIFE
                     GVNADNEVLKLISQSTANPEHLKILKQGVSAWNAWRQENPRVTPILSNADLHELTDMT
                     RVNLSGVIMHGVNLAGVNLESAEFNHANLSEANLAGVNLRTASLTGANLSKANLHGAN
                     LRGVSLIQADLSEADLSNANLDVIPDRDGDTEAFLSYATFRATNLSGASMRKAILRYS
                     NLVDANLTGANLTDADLYNADLVRSNLTNAILTGSDLSLADLVETNLTNAEISGCRIY
                     GASAWNVTLENTSQKDMVISRKDEPVVTVDDLEVGQFIYLMLNNTKIRDVINTITSKG
                     VLILGRFADPQRKAVLDGLREKLREFDLLPIVFDFDRPTDKDYTETVQTLAGMSMFVI
                     ADVTSPKSTPLELEATAKQFKIPFIPIIDISVDPRPFAMLVDLQKSFHWVLPTLQYQT
                     KEDLLDDENLKTYIIDRAVAKREELRAAKSQEPEMLLVRKKGTSTPSVTAS"
     gene            complement(2795..3280)
                     /locus_tag="PLG52_04015"
     CDS             complement(2795..3280)
                     /locus_tag="PLG52_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40642.1"
                     /translation="MTNELLLRDVSLDDLPIFFEQQRDEEANTMAAFTAKDPNDREAF
                     FAHWQRILADETVINKTIVVHGQVVGSVASYIDEGKPEVTYWLGKEYWGKGIATKALR
                     EFLAHHNQVRPIYARVAKDNLGSRRVLEKCGFTVIGESQGFANARGQEIEELLLERSA
                     A"
     gene            complement(3294..3797)
                     /locus_tag="PLG52_04020"
     CDS             complement(3294..3797)
                     /locus_tag="PLG52_04020"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB40643.1"
                     /translation="MNSLQNESLLTKLIEHNNWANLQIIQACSVLNDKQLDASPQSTT
                     KGSIRSTLLHLVASQRGYLSMLTQAEEAQSQTPLTFAELQASAEISGAGLLAVARDEA
                     TMSVKTKIQAKDGFIIVPWVIMVQVINHATEHREQIKSMLSDLGVSPPTIDGWSYGEF
                     TNAYTER"
     gene            complement(3929..4390)
                     /locus_tag="PLG52_04025"
     CDS             complement(3929..4390)
                     /locus_tag="PLG52_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40644.1"
                     /translation="MSTYSLRGISRIHSKGAGWLVRLYRNAKVITKLFSDGVYGDADK
                     SLRAAQVYYRQAQIKFPPIERPPFRETPLRNNTSGYNGICETYCRTRKGEKISCWNVS
                     WYNPPNKLNSKKFYFHDPQERKQALKEAIQFRKEREAEILKRSRSRRKIIS"
     gene            complement(4507..4968)
                     /locus_tag="PLG52_04030"
     CDS             complement(4507..4968)
                     /locus_tag="PLG52_04030"
                     /inference="COORDINATES: protein motif:HMM:NF025879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNB40645.1"
                     /translation="MKTMGLKIIGIAVRTTNKDGQSARDIGELWGQFYSQNLLETIPN
                     KLSNDIYSIYTDYKSDFTDEYTTLIGLQVSSLDTIPSGLIGRQFPTETFEVFTAKGEM
                     PQAVMNTWLDIWQRDNELQRKYTYDFEFYGEKSQNGENSEVQIFIATNRTQ"
     gene            complement(5010..5465)
                     /locus_tag="PLG52_04035"
     CDS             complement(5010..5465)
                     /locus_tag="PLG52_04035"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB40646.1"
                     /translation="MKTTTASPKGKSTTPTTSVKEFIASLDDEQSKADTHTLIEMMQR
                     ISGHAPKMWNVGTIGFDTYHYKYESGREGDSQTIGFYPRKGKLTVYLMDGTVRYSELL
                     SRLGKHTTSRVCIYIKRLSDIDLSILEQIVRQSYEYVKSQDGHMHRALE"
     gene            complement(5518..6000)
                     /locus_tag="PLG52_04040"
     CDS             complement(5518..6000)
                     /locus_tag="PLG52_04040"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNB40647.1"
                     /translation="MIKFRAKIEIIGVNPFVFLPEHALTEIFKQANTTKGKIPIKMKI
                     DGHEFTQTLIKWQGAWRLYLNMPMRKAAQKDVGDTADFEIIYDPVKRGFPMHPKLKKA
                     LQENPSAKEVYESLRPSLQLEINRYLSFLKTEESVDRNVIRAIQFLLGKGRFIGRDKP
                     "
BASE COUNT         1433 a         1414 c         1389 g         1878 t
ORIGIN      
        1 acttcatcat tattttcaag cacgagccat ggcaaagagg aagaaaatac atctgtcata
       61 cgagtactaa tttcatcggg agtaacgggt tctttttcaa atgtgataat cgtattcgca
      121 atgtaatggt tgtagatctc ggcgattttg acagcatctt ctgtctttgc tactcgaatc
      181 atcctgcgct cctgcaataa acgttttcag caatcaaata cgaaccaaca aatttcatta
      241 cgcagtttct ggtcatccag caccaatgcg cggatttgac tcggaagttg cgctcgttgc
      301 cattgacatt ccaatcgccc cgcttctttt gattcaccct ccaatgccgc tgcttggacg
      361 gctttgatgg cataggcggc ggctcccagc tcatgggcag ctacatgcgc caccgccccg
      421 gcttgacctg cggcaaaggc ggcgtgccga gccgcaccac tcaaggcgcg cgccgcagcc
      481 atggcatgtc cgcctgcggc acggctttgt gacatgctga tctcaccacg cgtccatgcc
      541 cgagcagact caattgcctg gcgcggacgg tcatcttgcg ggcagacatt ctcaaacaag
      601 tacaagacgt gttcggcaca atctgctgcc cataatgcca ataggtgatg gtctgaatct
      661 tgtaaggtgc cgccgcgccg tatggtgatg aatctagagt ctcgttgttt aggcaatatc
      721 atggcttggt ttaatgccgt tgtttaagac tttttctttt tagcggcttt tttgccattc
      781 aagttaagcg tgccagaggc atcgcgatgc gttccggtgg tttgttttac ggtaaattca
      841 gcaccgtgca aaatcacatg tgcaatggct ctggcatgcc cggtacccag accgtaatcc
      901 gtttttaacc aatcgagaat ctctttatgt gttgtcagct tctttttctt tgcctcagcc
      961 acgaattctt tcggggcttt gcctgtaagt tcttcaactt tatctaaata ggcttgaaac
     1021 gacatggttt tctcctgtaa gtgttaacac ctcaacttgc tgttacagac ggggtggatg
     1081 tgcctttttt acgaacgagc agcatttcag gctcctggct ttttgcagct cgtaactctt
     1141 cgcgttttgc aacggcgcga tcaatgatat aggtttttag attctcatca tccaacaggt
     1201 cttctttggt ttgatattga agggttggca aaacccaatg gaaagatttt tgcaaatcga
     1261 ccagcatggc gaacggacgt gggtctacgc tgatatcgat aatgggaatg aaagggatct
     1321 tgaattgttt ggcggtggct tccagttcta atggggtcga cttcgggctg gtcacatcgg
     1381 cgatgacgaa catgctcata cctgccaggg tttgaaccgt ctcggtatag tctttatcgg
     1441 tggggcggtc aaaatcgaag acgatcggca acaggtcgaa ctcgcgtagc ttttctcgca
     1501 gtccatccaa aacggctttg cgctgcgggt cggcgaacct gccaagaatg agtacacctt
     1561 tcgatgtgat ggtattgatc acatctcgga tcttggtatt gttcagcatc aggtaaatga
     1621 actgaccaac ttccagatca tctacagtga caaccggttc atctttccgc gaaatgacca
     1681 tgtctttttg agaggtattc tccaaagtga cattccatgc tgaagcccca taaattcgac
     1741 aacctgagat ctcagcgttg gttaaattag tttccaccaa atcagccagg ctgaggtcag
     1801 agccggtgag tatcgcgttg gtgaggttcg accggaccaa atcggcatta tagaggtcgg
     1861 catcggtcag gttggctccg gtcaagtttg catccactaa gtttgagtag cgcaaaatgg
     1921 ctttgcgcat gctggctccg ctaaggttgg ttgccctgaa cgtagcataa gataggaacg
     1981 cctccgtatc gccatcacga tctgggatga catccaaatt ggcgttgctc aaatccgctt
     2041 cgctgagatc tgcctggatt aaggacacgc ctcgcaaatt tgccccatgt aaatttgctt
     2101 tgctcaggtt cgctccggtt aaggatgcgg ttcgtaagtt gactccggca aggttcgctt
     2161 cgcttaagtt ggcatggtta aactctgccg attccaagtt gaccccggcg aggttaacgc
     2221 cgtgcattat gacgccgctc aagttgacgc gtgtcatgtc ggtcaactcg tgcaggtcgg
     2281 cattgctcag aatgggggtc actctggggt tttcttgccg ccaggcattc catgcgctaa
     2341 ccccttgctt aagaatcttc agatgttcgg ggttggctgt tgattgactg atgagtttaa
     2401 gaacttcatt atctgcattc accccttcaa aaatcgcacc ttccaaattt gcgtcactca
     2461 gcttcacccc aaacatatag gactggctta aatcggcacc ggtgagattg gctccgctaa
     2521 ggtctgaaga tgagaaatct gcattgcgca aataggcatt ggacagatta gctccgctta
     2581 aattaacatt gtataaacgt atgccaacca aattcatacc gctcaagtct gcgccgctaa
     2641 gatcaaagac cgtatcaaaa tttttgcttc ggagtgtatt ccatttctcc atgccttgct
     2701 ttagagcttc gagatgttcg gggtttgcca taggggatag gctcctcttt ttaaagtggt
     2761 tgcttttttc tcgatgatat cagttttgat gagcttacgc cgctgatcgt tctaaaagca
     2821 actcttcgat ctcctgaccg cgtgcgttgg caaacccttg ggattctccg atgaccgtaa
     2881 agccgcattt ttccaatacg cgccgcgagc cgaggttatc ttttgccaca cgcgcataga
     2941 tggggcgtac ttggttgtgg tgggctaaaa actctctcaa tgcttttgtc gcaatgcctt
     3001 tgccccaata ttcctttcca agccaatagg tgacttcagg tttgccttcg tctatgtaac
     3061 tggcaacact gcctacaacc tgcccgtgga caacgatggt cttattgatg accgtctcat
     3121 ctgccaaaat tctttgccag tgggcgaaaa aggcttcccg atcatttggg tctttggcgg
     3181 taaatgccgc catggtattg gcttcctcat ctcgttgttg ttcaaaaaag attggaaggt
     3241 catcgaggct aacatcccgc agaagaagtt cgtttgtcat gtcgtaaatt tgcctatctt
     3301 tctgtgtatg cattcgtaaa ttcgccatag ctccacccat caatagttgg cggggataca
     3361 cccaaatcgc taagcatgct tttgatctgc tcccggtgtt cagtggcgtg attgataacc
     3421 tgtaccataa tcacccaagg cacaatgatg aagccatcct ttgcctgtat ttttgttttt
     3481 acagacatgg ttgcttcatc tcttgccact gccagcaagc ctgcgccgct tatttctgca
     3541 gacgcttgca actcagcaaa tgtgagcgga gtttgagatt gtgcttcctc tgcctgtgtc
     3601 aacatcgata aatagcctcg ttgagaggcg acaagatgta acaaggtgct acgaatactg
     3661 cccttggtcg tagactgagg actagcgtcc agttgtttgt cattcaaaac agagcacgcc
     3721 tgaataatct gaaggtttgc ccaattgttg tgttctatta acttcgtaag aagactctcg
     3781 ttctgcaaag aattcatttt ctaagatttg ctcctcactt atcaattatt tgtaaatagg
     3841 ttatcttgta ttttatttat aacaatagcc gtcaaaacgg cacgaaactt ttttgtgcat
     3901 cattttgacg gctacgagct cacaaccttt atgatatgat ttttcgtcta ctacgagagc
     3961 gctttaatat ctcggcttct cgttctttac gaaattggat cgcttctttc aaggcttgct
     4021 tgcgttcttg aggatcgtga aaatagaact ttttactgtt caacttgttt ggcgggttat
     4081 accaactgac attccagcat gagattttct cgcctttgcg ggtgcggcag tacgtctcac
     4141 aaatgccatt gtaaccagag gtattattcc gaagtggcgt ttcccgaaaa ggaggtcttt
     4201 caatgggtgg aaattttatt tgtgcctgcc ggtaatacac ttgcgccgcc cttagtgatt
     4261 tatcggcatc tccataaacg ccatcgctaa agagttttgt gatcacttta gcattccgat
     4321 acaacctgac taaccagcca gccccctttg agtgtatcct gctgataccc ctcaaagagt
     4381 aagtactcat tcgattatca aattcctttc gtatgtaaga tgtgaaagga attatatctg
     4441 acaaattttg tagaatatag agagcggtta gcggggtttt gatcggttac ttggacagta
     4501 ttttgattat tgggtcctat tggttgctat aaagatctga acttctgaat tttctccatt
     4561 ttgagatttc tctccataga attcaaagtc ataggtgtat tttctttgca attcattatc
     4621 tcgttgccaa atatcgagcc aggtattcat aaccgcttgg ggcatttccc ccttggcagt
     4681 aaacacttca aatgtttcgg taggaaattg tctaccgatc agcccgctgg gaatggtatc
     4741 caaactgctg acttgtagcc ctatgagtgt tgtgtattcg tctgtgaaat cacttttata
     4801 gtcggtgtaa atagagtaaa tatcgttact caacttgttt ggaatggttt caagtagatt
     4861 ttgtgaatag aattgtcccc ataactcccc aatatctcga gcggactgac catctttatt
     4921 ggtcgttcga actgcaatac caattatttt gagtcccatt gttttcatta ggttttctcc
     4981 gctaccgata tgcgaaaagc atagattttt tactcgagcg cccggtgcat gtgtccgtct
     5041 tgagacttta cgtactcgta tgattgccgc acgatctgtt caaggattga caggtctatg
     5101 tcgctaagcc gtttaatata tatacagacc cggctggttg tatgtttgcc caaacgagac
     5161 agcaactcag aatagcggac cgtgccgtcc atgaggtaaa cggttagttt gccttttctt
     5221 ggataaaagc caatggtctg actgtcacct tcacgtccgc tctcatactt gtagtgataa
     5281 gtatcaaacc caatcgtgcc tacattccac attttgggcg catgcccgct gatgcgttgc
     5341 atcatttcga taagagtatg agtatccgct tttgattgtt catcgtccaa cgaagcaatg
     5401 aactctttga cagaagtggt tggggtagtg gacttgcctt ttggtgaagc ggtagttgtt
     5461 ttcataagtc ctaaaatttt agaacttatg ttctatttcg tcaagattga aatttactta
     5521 tggcttatct ctgccaataa atctaccttt tcctaatagg aactggatcg ccctaataac
     5581 gtttcggtcc acggactctt ctgttttcag gaagctcaaa tagcggttga tttccaattg
     5641 caatgaggga cgtaatgatt cgtagacctc ttttgcggat gggttttctt gcagcgcttt
     5701 tttgagttta ggatgcattg ggaagccgcg ctttacaggg tcataaataa tttcaaagtc
     5761 agcagtatcg cccacatcct tttgagccgc ttttcgcatc ggcatgttta gatacaaacg
     5821 ccaggctccc tgccatttta taagggtctg cgtgaactca tgcccgtcaa ttttcatttt
     5881 gatgggaatc ttgcctttgg ttgtgtttgc ctgtttaaaa atttctgtca acgcgtgttc
     5941 aggaaggaac acaaaagggt ttaccccgat gatttcgatt ttggctctaa acttgatcat
     6001 ggttatttga ctttggctag taaggtttca atagccgtta tatgttccag ctcatgcagg
     6061 gacattgaac gtgcctgcca atagacggat tctttccgct tttttccttt ctca
//
LOCUS       DAOQNJ010000089         6090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_7384_length_6090_cov_9.002486, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000089 DAOQNJ010000000
VERSION     DAOQNJ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7384_length_6090_cov_9.002486"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..856
                     /locus_tag="PLG52_04045"
     CDS             332..856
                     /locus_tag="PLG52_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB40648.1"
                     /translation="MSQDEISELISAAKSDPTAFGRLYDRYLQPVYRYLYSRLGDAHS
                     AEDVTSQTFITAYEALPKYREQGQFTAWLFRIARSKMYDYLRKNKREVELEAAGELLE
                     REDALGTLIRAEELSKVRFLIKQLNEEEQDLIRLRYVADLTFVEIADLLGKREDAVKK
                     TLYRLLARLKGQME"
     gene            859..2379
                     /locus_tag="PLG52_04050"
     CDS             859..2379
                     /locus_tag="PLG52_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HNB40649.1"
                     /translation="MNDQNPSPEFEEKVREAMHMPEADPKFAKRLRNELVGRPVKMKT
                     KIFRPAWAIAFVLALVVLAINVPRLAAALGRVFGYIPEVGLVETTNGLRMLAEPISST
                     RDGVTITITNVFAYPDRIELQYEVSGVAPEDDGWQSSDASSNPTAFCGGVNPGSTYNK
                     AGDARLRLPDGTVLERDYTGKYPKNVFAMQPVYDGALPAGVTSLTFVLDCIPQARLGV
                     VPEHWELALELVEVPAGTVVGEPVIEVTQPVVTEEVPASDSTETVSAPKVTITLERIV
                     PTDTNTVVYFHLNMENADPSLVSIMPRSVYVIDSLGQQFRMVGSFVWQPFEHKVGSAF
                     EFVSEGKPATGPLTVVVDEMTAYYMPMYVDPPQATPEEMTFTFDAGENPQYGQKWDLN
                     KTFTIAGYDFEIVSAQASNYDDVAKIHDYIDGSQGYEYGYQFAVTADPSLELLVEMDI
                     RSDEYQCWLSDVNKVETQPLLYTQLCKDGYPKGQVSVTIREMSIALDDDVQAEWIP"
     gene            2412..2795
                     /locus_tag="PLG52_04055"
     CDS             2412..2795
                     /locus_tag="PLG52_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40650.1"
                     /translation="MLKALQENMMNKSLIPFFLPFIIVACGSKGDEVPDVLLTPPAIV
                     VNASRDNCPSIEAEVGMTIAWENVDTVPLPIKIEFLDDNGKVTDTGLSVIDPETVFST
                     QFQEAGVYHVYCTENADTYSTITVK"
     gene            complement(2881..3453)
                     /locus_tag="PLG52_04060"
     CDS             complement(2881..3453)
                     /locus_tag="PLG52_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNB40651.1"
                     /translation="MTTLAPTIYNRTFPRVSEVVRDITLILLGSWLIALFAQIEIPLP
                     FSPVPITGQTFAVLLVGALLGSKRGAAAMVAYIAQGAAGLPFFAGGASGFGILTGATA
                     GYLVGFIVAAYVVGLLAERGMERSFKTSIVPFLVGTIIIYAFGVTWLSSVVGGFGKAI
                     ELGLLPFVVGDAIKLVAAAVALPAAWKFVR"
     gene            complement(3566..4813)
                     /locus_tag="PLG52_04065"
     CDS             complement(3566..4813)
                     /locus_tag="PLG52_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNB40652.1"
                     /translation="MILDETHPLEEVMVWGEPGIEALLGQLLPQSRSLFRSYYEVPKA
                     RGEFACMSSLIESEGIKVTRAKDAVADLLKTREIPELPGSIQDLKRILHQKADSTFEH
                     YRLAKQSDLIRDGITNISPLDVYNAVKRDIDVVLEEDIAQYGEQGALRLNYLLSLSKP
                     WPMANIFYGRDQSQALTDRIILSSLRWNIRKPEVRIFKDALHHMGYGTVLADIREGTI
                     EGGDVAMFKGYCFIGVGARTNINAVKEVYRQIGGSLKEHRIELIAVVNKQHEEDSNFF
                     ISPTSEHMQVMHLDMFWIPLTQDLVMAYTTELDHREVVRVWQEKGRILTERLGTFREF
                     MSIIGVEIFEVDEAEQKNFATNLLNFGNKKVIAALSSNPRVNAELERRGFTVKYAELT
                     KLVDGYGAVHCLTAPLKRRIPNS"
     gene            complement(4879..>6090)
                     /gene="argS"
                     /locus_tag="PLG52_04070"
     CDS             complement(4879..>6090)
                     /gene="argS"
                     /locus_tag="PLG52_04070"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNB40653.1"
                     /translation="HERGQWLLKLYVEANQLLEKKENETPEQTAQREAYEAERREMYR
                     KWDAGDEYVRELWRVTREWSLEELHEVLHMLDVKMDVWFYESEVDEPSKAIVEELIAK
                     NIADDERPNGGAVIVKIDEKLGLTKEKYRTNVILRRDGTTLYLTKDLALAKVKFEQYH
                     VDRSIYVVDSRQALHLQQAFAILKLWGFKQSEKCYHLGYGFVSLPEGAMSSRKGRVVL
                     FKEVFDEAVKRVLSVNAEKSVEIAADEREKVAAQIGLGALVYSMLSVDNNKDIVFNIE
                     EALSFDGRTGPYIQNAHVRATSILKKASKVSEADFNYDLTKQEIELIEQMSQFPQKVQ
                     QAAEEYRPLVMAMYAYDLANAFHSFYHAVPVLQSEDEKVKNARLRLVAAAKQAIANAL
                     HLLDIQSPDVM"
BASE COUNT         1559 a         1445 c         1453 g         1633 t
ORIGIN      
        1 ctgcggcgcc catgatgatg gttttgatcg gcataggaaa ctcctgtgca tgtcacggtg
       61 aggaacctgt cccgcttgtg gggcaatgtc cggcaaactc gaggtgggat ttgagcctgt
      121 ccgtttttac ccacgatgac atttacgatg cttaatatct aatctaaaag aaattacctc
      181 tctggtgaag taacgtttag cctgattttg gcatgacagt tttgtcctgt tttgattttc
      241 ggtgcgttta ctttatgaat gcatttacat acgcatcctc gcgagagaaa ggaactgcat
      301 gttaggattt gaagcagtaa agacgaaatt aatgagtcaa gacgaaatct ccgaactgat
      361 atccgcagcc aaaagcgacc ccaccgcgtt tggcaggttg tatgaccgtt atttgcaacc
      421 tgtctatcgc tatctctata gtcgccttgg tgatgctcat tctgcagaag atgttacatc
      481 gcagacattc atcactgcat atgaagcgtt gcccaagtat cgagagcaag gtcaattcac
      541 ggcgtggtta tttcgaatcg ctcgcagcaa gatgtatgac tacttgcgta agaacaagcg
      601 cgaggtcgag ttggaggcgg cgggcgagtt gcttgagcgt gaagacgcac tgggaacgtt
      661 gatccgcgcg gaagaattaa gcaaagtccg ttttctcatc aagcaattga acgaggaaga
      721 acaggatttg attcgcttgc gctatgtggc tgacctaacc ttcgttgaga ttgcagattt
      781 gcttggcaag cgcgaagacg ccgtcaaaaa gactctttac cgtttgttgg ctcgtttgaa
      841 gggtcagatg gagtgaatat gaacgatcaa aatccctctc ctgaatttga agagaaggtt
      901 cgcgaagcaa tgcatatgcc tgaagcggac cctaaatttg caaagcgatt acgcaatgaa
      961 ctggtaggga gaccagtaaa aatgaaaaca aaaatattta gacccgcttg ggcgatagca
     1021 ttcgtgcttg ctctagtagt tcttgcaata aatgttccga gattggctgc tgcattaggt
     1081 cgtgtgtttg gatatattcc cgaagtgggg ttggtggaaa caacgaatgg tttacgtatg
     1141 ttagcagaac cgatttcatc aactcgtgat ggcgtcacga ttacgatcac aaatgtattt
     1201 gcttatcctg atcgtattga attgcaatat gaagtgagtg gcgttgctcc tgaagatgac
     1261 ggctggcagt catctgatgc atcgtcgaac ccgactgcat tttgcggcgg cgtgaatcct
     1321 ggcagtacat ataacaaagc gggcgatgcg cgtttgcgtc tgcccgatgg cacggtgctt
     1381 gaacgtgatt acaccggcaa atatccgaag aatgtttttg cgatgcagcc tgtttatgat
     1441 ggtgcgcttc cggcgggtgt cacttctctc accttcgtgc tcgactgtat tccgcaagcc
     1501 cgtctcggcg ttgtgcctga acattgggaa ctcgcgcttg aacttgtcga agtcccggct
     1561 ggaacagtcg tgggcgagcc cgtgatcgaa gtgactcaac ctgttgtgac agaagaagtg
     1621 cccgcctctg actcaaccga aactgtctct gctcccaaag tgaccataac cctcgaacgg
     1681 atcgtcccta ctgataccaa cacagtcgtt tacttccacc taaacatgga gaatgccgat
     1741 ccttctttgg tatccattat gccgcgcagt gtttacgtca tcgactcgct cggccaacaa
     1801 ttccgtatgg ttggctcatt cgtctggcag ccttttgagc ataaagtggg cagtgccttt
     1861 gaatttgtca gcgaaggcaa acctgctacc ggtcccttga ccgtggtggt cgatgaaatg
     1921 accgcctatt acatgcccat gtatgtggac cctccacagg caacccctga agaaatgaca
     1981 tttacttttg atgcgggcga gaatccgcaa tacggtcaga aatgggattt gaacaagact
     2041 ttcaccattg ccgggtacga ctttgagatc gtctctgccc aagcttcgaa ctatgatgat
     2101 gtcgcgaaaa tccatgatta tatagacggt tcacaaggat atgagtatgg ctaccaattt
     2161 gccgttactg ctgacccatc tttggaactt ttggttgaaa tggacattcg aagtgacgaa
     2221 taccaatgct ggctgtcaga tgtcaataaa gtggaaactc aacctttgct ctatacccaa
     2281 ctttgtaagg atgggtaccc gaaaggacag gtttctgtca ccatccgcga aatgtccatt
     2341 gcattggatg atgatgttca agccgaatgg ataccatagg aactttatac cattcaggaa
     2401 aattcctttc gatgttaaaa gctttgcagg agaatatgat gaataaaagt ttaattccct
     2461 tttttctgcc tttcattatt gtcgcctgcg gttcaaaagg tgatgaagtg ccagatgtgc
     2521 tcctcactcc acctgctatt gttgtgaacg cttcgcggga caactgtcct tccatagaag
     2581 cagaggtagg aatgaccata gcttgggaaa atgtcgatac ggtgcctctg ccgataaaga
     2641 tcgaattctt ggatgacaat ggaaaggtta ctgacacggg attgtctgtg atcgaccctg
     2701 aaaccgtgtt ttcaacacaa tttcaagaag ccggggttta tcatgtctac tgtactgaaa
     2761 atgctgacac ctattcaacg attacggtga aataaaaaaa ctccccatac gcctgcaaga
     2821 atatatgcag gtgtatgggg agtaaataat tgtcttattc taggcgtgtg atgtttttag
     2881 ttatcggacg aatttccaag ctgcaggcaa tgctacagca gcagcgacta acttgatagc
     2941 atcaccaacc acaaatggta gcaatcctaa ttcgatagct ttaccaaaac cgccaacgac
     3001 ggagctcaac caagtgacgc caaaggcata gatgatgatc gtgccgacca aaaatggaac
     3061 aatggaggtc ttgaagctac gttccatgcc tcgttctgca agcagaccta caacatacgc
     3121 tgccacaata aagccaacga ggtaaccagc ggttgcgccg gtgagaatgc caaatccgct
     3181 ggcgccgcct gcgaagaaag gcaaccctgc cgcgccttga gcgatataag cgaccatggc
     3241 agctgcacca cgcttggagc caagcaaggc accgaccagc aatacggcaa acgtctgacc
     3301 agtaattggg acaggagaga aggggagtgg aatttcgatt tgagcaaaaa gcgcgatcaa
     3361 ccacgaaccg agtaagatga gggtgatatc gcgcacgacc tcagaaacac gaggaaaggt
     3421 gcggttgtag atggttggtg caagtgtggt catataaaat ctatctccta agtagaatgt
     3481 caatagttat aacacgcaaa cccttgtgaa gatttggata gggattaatg tcattctgtc
     3541 cccactgtag gatgacatgc catttttatg aatttggtat acggcgtttt agcggtgcgg
     3601 tgagacagtg gactgcgccg tatccatcga ccagtttggt caactcggcg tatttgaccg
     3661 tgaagccacg tctttccaac tctgcattca cgcgcgggtt ggaagataat gccgcgatca
     3721 cttttttgtt gccaaagttg agtaaattcg tggcgaagtt cttttgttcg gcttcgtcca
     3781 cttcgaagat ttctacaccg attatggaca taaattcacg gaaagttcct aatcgttccg
     3841 ttaagatacg ccctttttcc tgccatacac gcacaacttc gcgatgatcc agctcagtcg
     3901 tgtatgccat gaccaaatcc tgtgtaagtg gaatccagaa catgtccaaa tgcatgactt
     3961 gcatgtgttc gctggtcggg ctgatgaaga aattgctgtc ttcttcatgc tgcttgttga
     4021 cgacagcgat cagttcgatg cgatgctctt tcaacgagcc gccgatctgg cgatatacct
     4081 ctttgaccgc gtttatattc gtccgtgcgc cgacaccgat aaagcaatag cctttgaaca
     4141 tggcgacgtc gccgccttca attgtccctt cgcgaatgtc tgctagaaca gttccatagc
     4201 ccatatgatg tagcgcgtct ttgaaaatgc gcacctcagg ttttcggata ttccagcgta
     4261 gagaagaaag aatgatacgg tctgtgaggg cttgcgattg gtcacgtccg taaaagatat
     4321 ttgccatagg ccagggtttg gagaggctca gtaaataatt taggcgtaga gcaccttgtt
     4381 ccccatattg cgcaatgtct tcttcgagta caacatcaat atcccgtttt actgcgttgt
     4441 atacatctag aggggagatg ttggtgattc cgtctcggat caagtcgctt tgtttggcga
     4501 ggcgatagtg ctcaaaggta gaatccgctt tttgatggag aatccgtttc aggtcttgaa
     4561 tagagccggg taattcggga atttcacgcg tcttcaataa atctgctact gcatcttttg
     4621 ctcgcgtaac cttaatccct tcgctttcga ttaaggaaga catacaagca aattcaccgc
     4681 gtgctttggg tacttcataa taactgcgga ataggctgcg cgattgcggt aacagttgac
     4741 caagcaaagc ctcaatgccg ggctcgcccc aaaccatgac ttcttctaaa ggatgtgttt
     4801 catcaagaat catttatacc ttcacttata aaaacagact aaatcctcaa ggatttagtc
     4861 tgttttttgt actgattttt acatcacatc tggcgactga atgtctaata gatgaagcgc
     4921 attggcaatg gcttgtttcg ccgctgccac caatcgcaga cgggcattct taaccttctc
     4981 atcttctgat tgcaaaaccg gcaccgcatg atagaacgaa tggaaggcgt tcgccagatc
     5041 gtaggcatac atcgccatca caagcggacg atactcctct gccgcttgct gcaccttctg
     5101 cgggaactgc gacatttgtt caatcaactc gatctcttgc ttggtcaggt cataattgaa
     5161 gtccgcttca gaaactttgc tggctttctt caaaatggag gttgctcgca catgcgcatt
     5221 ctgaatatac ggacccgtgc gcccatcaaa agagagcgct tcttcgatgt tgaagacaat
     5281 atctttattg ttatccaccg aaagcatgga gtagaccaaa gcgcccaaac caatttgagc
     5341 cgcaactttc tcgcgttcat cagcagcgat ctccacgctc ttttcggcat tgaccgagag
     5401 cacgcgcttc acggcttcat caaagacttc cttgaaaagc accacccgtc ccttgcggga
     5461 agacatggca ccctcgggca ggctcacgaa accatagccg aggtgatagc atttttccga
     5521 ttgcttgaaa ccccataact tgagaatggc aaatgcctgt tgcaaatgca atgcctggcg
     5581 tgaatctaca acatagatcg agcgatctac atgatattgt tcgaacttca ctttggctaa
     5641 tgccaaatct ttggtcaggt aaagggttgt accgtcgcgg cgcaaaatga cgtttgtgcg
     5701 atacttctct ttggtcagcc caagtttctc gtcgatcttc acgattactg cgccgccgtt
     5761 cggacgttca tcatctgcaa tatttttagc aatcaactct tcaacgattg ctttcgaggg
     5821 ttcgtccact tcagactcgt agaaccacac atccatcttt acatcgagca tgtgtagcac
     5881 ttcgtgcagt tcttccaaac tccactcgcg ggtcacacgc cataactcgc gcacatattc
     5941 atcgcctgca tcccacttgc gatacatctc gcggcgttcg gcttcgtagg cttcgcgctg
     6001 agcggtctgc tcaggcgttt cgttctcttt cttttctaac aactgattgg cttccacgta
     6061 taacttcaag agccactgtc cgcgctcgtg
//
LOCUS       DAOQNJ010000090         6047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_7469_length_6047_cov_9.708945, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000090 DAOQNJ010000000
VERSION     DAOQNJ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7469_length_6047_cov_9.708945"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="PLG52_04075"
     CDS             complement(<1..1853)
                     /locus_tag="PLG52_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing serine/threonine
                     protein kinase"
                     /protein_id="HNB40654.1"
                     /translation="MAIPEKIGRYEIKEELGRGGFATVYRAYDPRFEREVAIKFLPSE
                     LMHSDPQFRMRFQREAKIIAQLEHPSIVPVYDVGAENEQPYFVMRYMSGGSLSERIKA
                     RTYTIEEAVKIIENLAPGLDEAHAKNIVHRDLKPANILFTEKNVPLIADFGIAKFTQG
                     DSTVDNMTGSAIIGTPAYMAPEQASGEAVDGRVDIYALGVILYEMVTGKQPYTADTPL
                     GLAIKHVTEPVPRILEANPNLPVWMEKVISTAMAKNPDDRFYSAVELVETIKAFLRGE
                     NPQINANVTKKMSPYNSTATFKRTKTAEPKRRNWVVPALVSVGFLAVAGVCAFALVSS
                     GMLQSLLEPVVTPTIAATSTISPATEVTETSEAITATVEVPPTENVPSLPLMGGADKI
                     AFIKNNDIWVMNVDGSELKQHTNDGLPKFNLQWMPDGKNLLYMTGKTVKTVNIDTEVE
                     EVLMNFVSAEYFEGFSVSPNGKQAAISINRELFIVPFEIETLRAATRKSALLEMKGCF
                     YNDLGIKELQWSDDSTKLAVEFIANSNGSFADAIRVYDVQDCEKISNSKIDEFPAGRF
                     QFSGDLSSFDWDGDLLFFLTSNTRNGGFGDLALYNIFTHKAEKAAPYESNC"
     gene            complement(1879..2508)
                     /locus_tag="PLG52_04080"
     CDS             complement(1879..2508)
                     /locus_tag="PLG52_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40655.1"
                     /translation="MSKLTPKIILEGTRLTFKTDIAFALNEHPRIVGPRKYKYHSPLV
                     SGEWCAFTNFPWGRGLINFEPQEESLAMETYNTWVKLFELQKYYSWIVDRFHLSTRMY
                     QLMTYQKDYDFRWLEERLLPLNFRLVLMTRSPESFAAARAERLKVSGNPSQYDDLNLF
                     VEEQKMIRKLFNDSILPKIEIDISDNNISAAVEHIADWMESTGGLYMAE"
     gene            complement(2505..3380)
                     /locus_tag="PLG52_04085"
     CDS             complement(2505..3380)
                     /locus_tag="PLG52_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB40656.1"
                     /translation="MMKKLAFLSLLLVSSLACATIEKALTSGATPTPIVIPTIAPSQT
                     AVPIATDIIRKEESDIFCPSNVTEAVDAYNKGLTYENAGDTASAEKAYRDAIALDPNY
                     CDAMDNLALLVRKNGNSDEAIALYENSIKVAPDNQVAHLGLANTYMELEEYDKALAEY
                     NAILAINPENPEGYYGAGRVYFSKEDFKSAIPQFKQAEELYKAEGSSYLIDAQVYLGF
                     SYALIDEFETGRDYLEMVYPQMQDNGYVNYFLGYCYYYGTSIRDEKLAKQYLLRARDL
                     GIDLGSDLETFVNTP"
     gene            3523..3888
                     /locus_tag="PLG52_04090"
     CDS             3523..3888
                     /locus_tag="PLG52_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40657.1"
                     /translation="MDDKITIIEGPPPVFEHVNDGWAMGLNESPNLSIPALTRLRTFN
                     GPALVQRCYNAWHNKTSIHLHYRNETGLEQTAPILAARNVETPEGHVLLLWVYLDSEK
                     VEYETDDASDDDLDDYPGE"
     assembly_gap    3914..4013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4014..4682
                     /locus_tag="PLG52_04095"
     CDS             <4014..4682
                     /locus_tag="PLG52_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB40658.1"
                     /translation="QNALTDAEKDSQVRCVVITGNGKVFSAGQDISEMKKGGGEISYA
                     EHLEKTYNPLILQIREMGKPVVAAVNGACAGAAFGIALACDLRIAHTNAYFVVGFSGI
                     ALAPDSGVSLLLPKLIGLGRAQEYFYTNSPIPAQLAFNWGIVNRLGGFNYQRVVDQMM
                     GDLAQGPREAFALGKKAFNEAVLPNLASVLHGEGILQDVAGKSSEHQVAVKAFFEKRK
                     PKYD"
     gene            4907..>6047
                     /locus_tag="PLG52_04100"
     CDS             4907..>6047
                     /locus_tag="PLG52_04100"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNB40659.1"
                     /translation="MSGQINAFEMAQKQFDAVAKQLKLDSGVAETLRWPMREYAFRIP
                     VRMEDGSLKVFQGFRVQHNDARGPNKGGIRFHPAETLDTVRALATWMTWKCAVADIPL
                     GGGKGGIIVDPSTLTVREKEDLCRGWVRNMWKNIGPRNDVPAPDVGTTPQMMGWMMDE
                     YSKLVGHYTPGVFTGKPLGGGGSLGRTEATGYGVIYTVREAMKRLNIDPKKSVAVLQG
                     FGNVSQYAAIGFVEILGGTVACVSCYDRHDKKAYTYSKKGGIDPRFLLSIVDEYGTID
                     KKKALDSGYQVDDGDKWIEFEGNVLIPAALEGQVNGDTVKKVSKGVKIVAEGANGPTT
                     PEADEVFKKNGVFNIPDFLCNAGGVTTSYFEQVQNDMNFYWSKDEV"
BASE COUNT         1465 a         1413 c         1513 g         1556 t
ORIGIN      
        1 caacaattgc tttcatacgg cgcggctttt tcggctttgt gggtgaagat gttgtataac
       61 gccagatctc cgaacccgcc attgcgtgtg ttgctggtga ggaagaagag caggtcacca
      121 tcccaatcga aactggagag gtcgcctgag aattggaaac gcccagcggg gaattcatcg
      181 atcttggagt tgctaatctt ttcgcaatct tgcacatcat acacacgaat ggcatcggca
      241 aagctgccgt tggaattcgc aatgaactca actgctagtt tggtgctgtc atcagaccat
      301 tgaagttctt tgatgccaag gtcgttataa aaacaaccct tcatttcgag taaagcgctt
      361 ttgcgagttg cggcacgcag tgtttcaatc tcaaatggca caatgaataa ttcacggttg
      421 atgctaatag ctgcttgttt accattgggc gaaacactga aaccttcgaa gtattctgct
      481 gaaacaaagt tcatcaatac ttcttctact tctgtatcaa tgttcaccgt cttcaccgtt
      541 ttaccggtca tataaagaag gttcttgcca tctggcatcc attgcaaatt aaactttggc
      601 aagccatcgt tggtatgttg ttttaattca ctgccatcta cattcatcac ccaaatatca
      661 ttattcttga tgaatgcaat cttatcggcg ccgcccatca atggcaggct cggcacattt
      721 tcggttgggg gcacttcaac agttgctgtg atggcttcgg atgtttcagt cacttcagta
      781 gcaggtgaga ttgtagatgt ggcagcaatg gtcggagtaa caaccggctc taacaaagac
      841 tgaagcatgc cgctgctaac gagggcaaaa gcacatacgc cggcaactgc gagaaaccca
      901 actgacacca aggcagggac gacccaattg cggcgtttgg gttctgcggt tttggttcgt
      961 ttgaaagtcg ccgtgctgtt ataaggcgac attttcttgg tcacattggc attaatctgc
     1021 ggattctctc cgcgtagaaa tgccttgatg gtttcgacca attccactgc agaatagaag
     1081 cggtcatctg ggtttttcgc catcgccgta gagatgacct tttccatcca gaccggtaga
     1141 tttgggttgg cttctaaaat acgcggcaca ggttctgtga cgtgtttgat cgccagtcca
     1201 agtggagtgt cggcagtgta gggctgtttg ccagtgacca tttcatacaa gattacgccc
     1261 aaggcataaa tatccacacg tccatccaca gcttcgccag aggcttgctc gggtgccatg
     1321 tacgctgggg tgccaatgat ggcagagccg gtcatgttat cgaccgtgga gtcaccttga
     1381 gtgaattttg caatgccgaa atcggcaatc aatgggacgt tcttttcggt aaaaagaata
     1441 ttggctggct tcaggtcgcg atggacaatg ttcttggcat gcgcttcatc caaccctggc
     1501 gcgagatttt caatgatctt gacggcttct tcaatggtat aggtccgcgc tttgatgcgt
     1561 tcagagagtg accctccact catgtagcgc attacaaaat aaggctgttc attttcagcc
     1621 cctacatcgt agaccggcac aatggaggga tgttccagtt gggcgatgat ctttgcctca
     1681 cgctggaaac gcatccggaa ttgtgggtcc gaatgcatca gctcagatgg cagaaacttg
     1741 atcgccacct cgcgttcgaa gcgcgggtcg tacgcccgat agaccgtggc aaacccacca
     1801 cgcccgagtt cctctttgat ttcgtaacgc ccgatcttct ctggaatagc catgcgagca
     1861 agtataccga tgaagtcttt attctgccat gtacaacccg cccgtggatt ccatccaatc
     1921 ggcgatgtgt tctacggcag ccgaaatgtt gttatctgag atatcgatct cgattttggg
     1981 caggatcgaa tcattgaaca acttgcggat catcttctgc tcttccacaa agagattcaa
     2041 gtcatcatat tgagagggat ttccagagac cttcaagcgt tcggctcgag ctgcggcaaa
     2101 agattcaggt gagcgcgtca tcaagacgag gcgaaaattg agcgggagga gacgttcttc
     2161 cagccagcgg aagtcataat ctttttgata cgtcatgagt tgatacatgc gtgtggaaag
     2221 atgaaaacgg tccacgatcc atgaatagta tttttgcaac tcgaaaagtt tgacccatgt
     2281 attgtaggtc tccattgcta gtgactcttc ctgcggctca aagttgatca gcccgcgccc
     2341 ccaggggaag ttggtaaagg cgcaccattc tccagaaacc agcggcgagt gatacttata
     2401 cttgcgcgga ccgacaatgc gcggatgttc gttgagtgca aacgcaatat cggttttgaa
     2461 ggtcaggcgt gtcccttcga ggatgatctt cggtgtgagt ttgctcatgg tgtgttgacg
     2521 aaagtctcca agtctgatcc aaggtctatg ccgaggtcgc gggcacgcag caggtattgt
     2581 tttgccaact tctcgtcacg aatggatgtg ccataataat aacagtagcc aaggaagtag
     2641 ttaacatagc cgttatcttg catttgcggg taaaccattt caagataatc gcgcccggtt
     2701 tcgaattcat cgatcaatgc gtatgaaaaa ccgagataca cctgtgcatc gataagatag
     2761 ctcgaccctt cggctttata aagctcttct gcttgtttaa actgaggaat agcagacttg
     2821 aaatcttctt tggagaaata caccctgcct gccccgtaat agccttctgg gttttctggg
     2881 ttgatcgcca agatcgcatt gtattcagcc aatgctttat cgtattcttc aagttccatg
     2941 taggtgtttg ccaaccctaa gtgcgctacc tgattatcgg gtgctacttt gatggagttt
     3001 tcatacaaag caatcgcctc atctgaattt ccatttttgc gtaccaaaag agccagatta
     3061 tccatggcat cgcaataatt cgggtccaat gctatggcat ctcgataggc tttttctgct
     3121 gaggcagtat cgcctgcgtt ttcataggtc aaccctttgt tgtaggcgtc cacagcttct
     3181 gtcacattgg atggacaaaa aatatcagat tcttcttttc gaatgatatc ggtggcaatg
     3241 ggcaccgcag tttgggatgg ggcaatggta gggatcacaa tgggtgtggg cgttgcgccg
     3301 gaagtcaacg ctttttcaat cgtggcacat gccagcgaac ttaccaaaag aagagataaa
     3361 aaagccaatt ttttcatcat ttgcctccag tttgtatgtg aaaattatct cattcaatgc
     3421 gcccagaacc tatagtctat ttgtcctgat ttcgattctg ataaatttct gaaacgattc
     3481 gacaacccag ttgtggtata ttcccgccaa ggtaaattta ttatggacga caaaattacg
     3541 atcatagaag gtccgccccc cgtttttgag cacgtcaacg acggatgggc aatgggcttg
     3601 aacgaaagcc cgaatctctc catccccgcg cttacgcgcc tgcgcacgtt taatggtccc
     3661 gccttggtgc agcgctgtta taacgcgtgg cataacaaaa cttccatcca cttgcattac
     3721 cgcaacgaaa cgggcttgga acaaaccgct cccattcttg cagcccgcaa tgtggaaacc
     3781 cccgaagggc acgtcttgtt gttgtgggtc tatctcgata gcgaaaaggt cgaatacgaa
     3841 accgacgacg ccagcgacga cgatttagac gattaccccg gcgaataaag ttgattacaa
     3901 tcagtgacag tcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaaaatg
     4021 cgttgaccga tgccgaaaaa gattcacagg tgcgttgtgt tgtcattacc gggaatggaa
     4081 aagtattcag tgctgggcaa gatatttctg aaatgaaaaa aggcggcggg gagatttcgt
     4141 atgcagaaca tttggagaaa acctataacc cactcatttt acaaattcgt gaaatgggca
     4201 aacctgttgt cgctgcggtt aatggagcgt gtgcgggcgc tgcctttggc atcgctttag
     4261 catgtgattt gcgtattgcc cacaccaatg cgtattttgt ggttggtttt agtggcattg
     4321 ctcttgcgcc cgattctggc gtttcattgt tgttaccaaa actgatcggg ttggggcgtg
     4381 ctcaagaata tttctatacc aacagcccca ttccggcaca attggccttt aattggggca
     4441 ttgtgaatcg gcttggcgga ttcaactatc agcgtgtggt agaccaaatg atgggcgacc
     4501 tggctcaagg tccgcgtgag gcgtttgcat tggggaagaa agcgttcaac gaggcggtct
     4561 tgcccaactt ggcaagcgtc ttgcacggag agggtatcct gcaagatgta gcgggaaagt
     4621 cgtctgaaca tcaggtggcg gtaaaagcgt tctttgagaa gcgtaaaccg aaatatgact
     4681 agcgtatcct atttgggggt ggcagggtta ttacatggcg tcagaaatca gcgtactttt
     4741 gtcatattga cgcaaaaatg aatctcttgt atagttttag agtagtattc gtggaattac
     4801 cgatcttatg aaccagcgtt cttacatagg ctgtgaataa gcgggcaccc cgtccggagt
     4861 gcccgcttat attttctctt aaacctctac ctaggagagt aaaaaaatgt caggacagat
     4921 caatgcgttc gaaatggcac agaagcaatt tgatgctgtt gccaagcaat tgaagctcga
     4981 ctctggcgtg gctgaaaccc tgcgttggcc gatgcgcgaa tatgcgttcc gcatccccgt
     5041 tcgcatggaa gatggctcgc tcaaggtctt ccaaggcttc cgtgtgcagc ataacgatgc
     5101 gcgcggacct aacaaaggtg gtattcgctt ccacccggct gagacgctcg atactgtgcg
     5161 tgcgctcgcc acatggatga cctggaaatg cgccgtggca gatattccgc tcggcggcgg
     5221 taaaggtggc attattgttg acccctccac cctcaccgtg cgcgagaagg aagacctttg
     5281 ccgtggttgg gtacgcaata tgtggaagaa tatcggtcca cgcaatgacg tgcccgcccc
     5341 cgatgtaggt accactccgc aaatgatggg ctggatgatg gacgaatatt ccaaactcgt
     5401 cggtcactac acccctggcg tcttcactgg caaacccctt ggcggcggcg gctcgctcgg
     5461 ccgtaccgaa gccacgggtt atggtgtcat ctacaccgtc cgcgaagcga tgaagcgcct
     5521 taacatcgac ccgaagaaat ctgtggctgt gcttcagggc ttcggaaatg tgtcgcagta
     5581 tgcggcaatc ggtttcgttg aaattctcgg tggtaccgtg gcttgtgttt cttgctacga
     5641 ccgccatgac aagaaagcct acacctatag caagaagggc ggcattgatc cgcgcttcct
     5701 gctctccatc gtggatgaat acggcaccat cgacaagaaa aaggcgctgg actctggcta
     5761 ccaggtggat gatggcgata aatggatcga gttcgaaggg aatgtcttaa tccctgccgc
     5821 tctcgaagga caggtcaacg gtgatacggt caagaaggtc tccaagggcg tcaagatcgt
     5881 tgctgaaggc gcgaatggtc ctaccactcc ggaagccgac gaagtgttca agaagaacgg
     5941 cgtgttcaac attccagact tcctctgcaa cgctggcggc gtgaccactt cttacttcga
     6001 acaggttcag aacgatatga acttctactg gagcaaggat gaagtcc
//
LOCUS       DAOQNJ010000091         6008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_7575_length_6008_cov_9.334789, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000091 DAOQNJ010000000
VERSION     DAOQNJ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7575_length_6008_cov_9.334789"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..599)
                     /gene="rnpB"
                     /locus_tag="PLG52_04105"
     ncRNA           complement(149..599)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLG52_04105"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            777..1922
                     /gene="coaE"
                     /locus_tag="PLG52_04110"
     CDS             777..1922
                     /gene="coaE"
                     /locus_tag="PLG52_04110"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNB40660.1"
                     /translation="MSNIPGKFVIGLTGNIATGKSVVRRMLEHLGAYTIDADSLTHRS
                     YAKGAPGYQQVLDKFGKWLLNREGEIDRQKLGNLVFGDPEALRQLEEIVHPLVRQATE
                     MLIKRASQPVVVIEAIKLLEGDLKKSCDSIWVTNAPEVIQVERLMRKRGFTREQAQER
                     IHAQSPQSQKVNMANMVITNTGSYDDLWKQVNEAWKQVVPGASDTQAETITIKKSTGT
                     GELTAHRGKPKHAASIADLVTRLSKGKRKMSSDEVMESFGDKAYMLLQMDGQVVGVAG
                     WQVENLVTRTTDIFLEDNLDLSKALDTLVKGVEGASGELQSEASLIFAMDNLAAQDAT
                     WQKLGYEKRTPDSLGVQAWQEAAEEAAPAGSVLFFKQLRQDRVLRPI"
     gene            1995..2867
                     /gene="cdaA"
                     /locus_tag="PLG52_04115"
     CDS             1995..2867
                     /gene="cdaA"
                     /locus_tag="PLG52_04115"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNB40661.1"
                     /translation="MTEFLNNLFFIFQRVSWLSVLDIMLVTLIFFALLYSLRDTQAMA
                     LLRGMIMIIVAITLITSLVDLPAFSWFARNALPSLLIAIPVIFAPEIRRALERLGRAG
                     TFLPATAKTADSALEETIRAIVGAAARLSARKHGALIVMQRLDHLDDYIQTGVQLNAK
                     VTPELFLQIFYPNTPLHDGGVIVVDSKVVAASCVLPLSASGILNRTPERQMGLRHRAS
                     LGISEVSDAIAIVVSEETGAISIAHSGRMLRRLDPERLENILTAFFRPSGAEQRIGFI
                     GKLFSGKKSRKEEK"
     gene            2871..4124
                     /locus_tag="PLG52_04120"
     CDS             2871..4124
                     /locus_tag="PLG52_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNB40662.1"
                     /translation="MVRWILENYRTFLWAFALAVAVWISSVTSADPDETRALDNPISV
                     EVIGQSSNLVFSEPIAKEVSVTLRAPSSVWALIDDDPSIVRAILDVSGLSAGEHMVDL
                     QIQVNARPVQIVSVSPKTFVFSLEPLITKSMDVDLRVAGEVAVGYQVGDSTLDPVSVV
                     VSGAQSQVEKVSRARVSIDLNRIRENFDQAVKVEVLDDRGQVVNGVTVSPESVHIVLP
                     VSQQGGYRDVAVKVTTLGRVASGYRLTDISVSPPVVTIYSTDPDLVNSLPGVLETQPL
                     DLQNAQDDIATRLKLSLPSGISVIGEQTVFVQAGVSPIESSVTISSENIEILGLDESL
                     NVQISPSNVDIILSGPLPLLETLTRQSVRVTVDLTGLTEGKYQLSPNVEVLVANVAVE
                     SILPNTIEVTLTPLNSSTPTPTPKP"
     gene            4140..5504
                     /gene="der"
                     /locus_tag="PLG52_04125"
     CDS             4140..5504
                     /gene="der"
                     /locus_tag="PLG52_04125"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNB40663.1"
                     /translation="MKPIVALVGRPNVGKSTLFNRLVGERMAIVDDTPGTTRDRLFGD
                     AEWNARPFTVIDTGGIDPTHGGRTPLSVGSADFIGDIRRQAQAAIQEADAILFVVDGE
                     TGVTEPDREVADILRRMQKKQPDGSFLPPIILVVNKCESRERRDSVGEFYELGLGEPF
                     AISAIHGTNTGDLLDALVSSFPTQNEDEEDNNIKIALVGKPNAGKSSLLNKLVGEERV
                     IVSPIAGTTRDAIDMELEFGGLPITLIDTAGIRRRGKIEKGVEEYSVIRSFKAIERAD
                     VALLMIDATTGITSQDAHIAGFIKDEWKSCVVLVNKWDAIEKDGTTMDAYTEKILNDL
                     NFVSYVPILYISAKTGQRVDQVLPLALRVQEERLARLTTSKINEIIHAAQDAHPHPTH
                     AGRALKMYYGTQVRSDPPTFMIYVNEPSLMHFTYMRYLENQIRAEYGFLGTPIRIVLK
                     GRHE"
     gene            5497..5877
                     /locus_tag="PLG52_04130"
     CDS             5497..5877
                     /locus_tag="PLG52_04130"
                     /inference="COORDINATES: protein motif:HMM:NF022700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 family protein"
                     /protein_id="HNB40664.1"
                     /translation="MNKKNILITGDVLAFAILNIIGFATHKETGVSFLPRMAVAFFPI
                     VFAWFVLAPWFGLFDETVINNSKNLFRIPLAFLFVAPLAVILRGALLNAPALPLFALV
                     FGSTNAFGMLVWRWLYLVLFAKKA"
BASE COUNT         1450 a         1545 c         1495 g         1518 t
ORIGIN      
        1 actgccgata gagtggcaaa caggtctggg gttggggtgg aggcggaagg caaattgcaa
       61 gcaaagacga agacaatcaa gaatagtatc caccatgcag ggcgtttggt cttcatccga
      121 tttaaagaat tcatccaata aaaaaatcgc ttcaggctgg tctataagcc gcattctgtc
      181 caatggcttg cgccatttgg gcaatcatct ctctgggcgg tgcgtcaccg cgccgctcgg
      241 tgcagcctac ccgggactga cccaacccac aggagggaag ggtgcgtatg gaggcgagca
      301 gcctcccatc gtccacagac gaaatcgtcc ctgcttggcc ttgctcccgg cgggggttac
      361 ctggccacgc acattactgc gcgcgccggt ggtctcttac accacctttt caccatcact
      421 gccaaaggca gctgtttgtt tctgtggccc ttttccggta ggttcatgcc tttcggcaat
      481 ctttccgtca acgaatgacg gacttcccca ccccgggtgc tatccgacgc cgtgctctaa
      541 aggagtgcgg actttcctcg atcccgtcaa cgcagaaccg cgattgccca accaacctga
      601 agcgtttgca tgatactcgt ggagagaggg agagtcaacc gcgcagagcc gattttgctc
      661 ttttctttat acaattttgc aaaagtcatg cagttttcat tgccaaaggt ggacatttct
      721 ggaaaagaca ttaaaattga aaggtcatat aatttttgtc tcctagagga atccctttga
      781 gcaacatacc tggaaaattc gtcattggtc tcactggtaa catcgccacc ggcaagagtg
      841 tggtgcgccg catgctggaa cacttaggcg catacaccat cgatgccgat tctttgacgc
      901 atcggtcgta tgccaaaggc gcgccgggtt atcagcaagt gctcgataaa tttgggaagt
      961 ggttgctcaa tcgtgaaggg gaaatcgatc gccaaaaact tggcaattta gtgtttggtg
     1021 accctgaagc cctgcgacaa ttggaagaga tcgtccaccc gctggtgaga caagccaccg
     1081 agatgctcat caaacgcgct tctcaacccg tggtggtgat cgaagcgatc aaacttctcg
     1141 aaggcgactt gaaaaaatcg tgcgactcga tctgggtcac gaatgcgccg gaagttatcc
     1201 aagtggagcg cttgatgcgc aagcgtgggt ttacccgcga acaggcacag gaacgcattc
     1261 acgctcaatc accgcagagt caaaaagtga atatggcaaa catggtcatc acgaataccg
     1321 gttcgtatga tgatctgtgg aagcaagtca acgaagcatg gaagcaggtc gtccctggtg
     1381 caagcgatac acaagcagag acgattacca tcaaaaaatc taccggcacc ggtgaactca
     1441 cggcacaccg tggcaagccg aagcatgccg cgtccattgc agacttggtc acacgcttaa
     1501 gcaaaggcaa gcgcaagatg agttctgatg aagtgatgga aagctttggt gacaaagcct
     1561 acatgctcct tcaaatggac ggtcaagttg ttggtgtggc tggctggcaa gtagagaatt
     1621 tagtaacacg cacaaccgat atcttccttg aagataatct tgacctttca aaagcactcg
     1681 atactctcgt caagggagta gaaggggcat caggtgaatt gcaaagcgaa gcatcgttga
     1741 tcttcgccat ggataatctt gctgcacagg acgccacctg gcagaaactc ggatatgaaa
     1801 agcggacacc cgatagcctg ggtgtgcaag catggcagga agctgccgaa gaagcagccc
     1861 ccgctggaag tgtgctcttc ttcaaacaat taaggcagga ccgcgtcctc cgcccgatct
     1921 aagctgttac aacctagtgg atttggggta catcccgaac gaacagctga ggcggagttc
     1981 cgtcccattt acacgtgact gaatttctga ataacctctt ctttattttc caacgtgtca
     2041 gctggctgag cgtgttagac atcatgctcg tcacgctgat tttctttgcg ttgctctact
     2101 ccctgcgtga tacacaagcc atggcattgc tacgcggcat gatcatgatc atcgtcgcca
     2161 ttacgttgat caccagcttg gttgacctac ccgccttttc ctggtttgcc cgtaatgccc
     2221 tgccctcttt gttgattgcc attccagtga tctttgcccc agaaattcgg cgcgcattag
     2281 aacgcctcgg tcgtgccggg acctttttgc ctgccactgc taaaacagct gactctgctc
     2341 tcgaagagac catccgcgct attgtgggtg ccgctgccag actctccgcg cgcaagcatg
     2401 gtgccttgat cgtgatgcag cggctagacc atctcgatga ttacattcag accggtgtgc
     2461 aattaaatgc caaagtcaca ccggaattat ttttgcagat cttttacccg aacacgcctt
     2521 tgcatgatgg cggcgtgatc gtagtggatt caaaagtcgt ggcagcatct tgtgtgctgc
     2581 cgttatctgc cagcggcatt ttgaaccgca ctcctgaacg tcaaatggga ttgcgacatc
     2641 gtgcctcgtt ggggatttca gaagtgagtg atgcgattgc catcgtagtt tcagaagaga
     2701 ccggcgccat ttccattgca cattctggta gaatgctccg ccgcctcgac ccggaacgcc
     2761 ttgaaaatat tctgacggcg tttttccgcc cgagcggtgc agaacagaga attggtttta
     2821 tcggtaagct attttccggc aaaaaaagcc ggaaggaaga aaagtagaac atggttcgtt
     2881 ggatccttga gaattaccgt acttttttat gggcattcgc attggctgtt gccgtgtgga
     2941 tctcttcggt tacctcagca gacccagatg aaacccgcgc gttagataac ccgatctcag
     3001 tagaagtgat tggtcagtcg agcaatcttg ttttcagtga gccaattgca aaagaagtct
     3061 ctgttacttt gcgcgcccca agctcagtat gggcattgat tgatgacgac ccaagcattg
     3121 tccgcgctat tcttgatgtc tctgggctca gtgctggtga gcacatggtg gatttgcaaa
     3181 tacaggtcaa tgctcgcccg gtgcagatcg tttctgtttc gccaaagacc tttgtattct
     3241 ctctggaacc gcttatcacc aaatcgatgg atgtggattt acgtgtggcg ggtgaagtgg
     3301 ctgtgggata tcaagtagga gattccactc tggacccagt atccgttgtg gtgtctggcg
     3361 cccagtctca ggtcgagaaa gtgagccgcg ctcgtgtgtc cattgacctg aatcgcatcc
     3421 gcgagaattt tgatcaagcg gtgaaagtcg aagtgttgga tgatcgcgga caagtagtga
     3481 atggcgttac agtttcgcct gaaagcgttc atattgtgtt gcccgtcagc cagcaaggcg
     3541 gttatcgcga tgtagccgtc aaagtgacta cgctcgggcg tgtggcgagc ggatatcgcc
     3601 tgaccgatat ttctgtctca ccgccagtgg tcacaattta ttccaccgac cctgatttgg
     3661 tgaacagcct gcccggcgtg ttggaaaccc agccgcttga tctgcagaac gcacaagatg
     3721 atattgctac gcgtctgaaa cttagcctgc cttcgggcat ttccgtcatt ggcgagcaga
     3781 cggtttttgt acaagctggt gtgtcgccca ttgaaagtag tgtgaccatc tccagcgaaa
     3841 atattgaaat ccttgggctg gatgaaagtt taaatgttca gatctcgccc tccaatgtag
     3901 atattattct ttcgggtccg ttgccgctct tggagacact cacacgccaa tcggttcgcg
     3961 tgaccgtgga cctgactggg ctgaccgaag gcaaatatca actcagcccc aatgtggaag
     4021 tgctggtagc aaatgtcgct gttgagtcga tcttgcccaa caccatcgaa gtgacgctga
     4081 ctccgctaaa ttcgagcacg cccacaccaa cccctaaacc ataaagtaaa ataaacccta
     4141 tgaaacccat tgttgcacta gtcggccgac ccaatgtcgg taaatccacc ctgttcaatc
     4201 gcctcgttgg cgaacgcatg gcaatcgttg acgatacccc cggcaccacc cgtgaccgcc
     4261 ttttcggtga tgcagagtgg aacgcccgtc ccttcactgt tattgataca ggcggtatag
     4321 acccgactca cggcggtcgg actccgctct cagttggttc tgccgatttt atcggagaca
     4381 tccgccgaca agcccaagct gccattcaag aagccgacgc catcctcttt gtcgtcgatg
     4441 gcgaaaccgg cgtcactgag ccagaccgcg aagtggcaga tattttgcgc cgcatgcaaa
     4501 agaaacagcc cgatggttct tttctgcctc cgatcatatt agtcgtaaac aaatgtgaat
     4561 ctagagaacg ccgtgattct gtaggtgaat tttatgagct aggtttaggg gaaccctttg
     4621 ccatttctgc cattcacggc accaacaccg gtgacttact cgatgcactc gtctcttcgt
     4681 tcccgactca aaatgaagat gaagaagata acaatatcaa gatcgccttg gtgggcaaac
     4741 ccaatgccgg gaaatcaagc ctgctcaata aactcgttgg cgaagaacgt gtgatcgtca
     4801 gcccaattgc agggaccacc cgtgatgcca tcgacatgga attagaattt ggcgggttgc
     4861 ccatcactct cattgatacc gcgggtatcc gtcgccgtgg caagattgaa aaaggcgtgg
     4921 aagagtacag cgttatccgt tctttcaaag ccattgaacg cgcagacgtt gcgttactga
     4981 tgatcgatgc caccaccggt atcacctcac aagatgccca cattgctggt tttatcaaag
     5041 acgagtggaa atcttgtgtg gttttggtca acaaatggga tgccattgaa aaagatggca
     5101 ccacgatgga tgcctacaca gagaaaattc tgaacgacct gaattttgtt tcgtacgttc
     5161 ccatccttta tatctccgcc aagaccggtc agcgcgtgga tcaagtctta cccttggcat
     5221 tgcgtgtcca agaagaacgc ctcgcccgcc tgacgacttc gaagatcaat gaaatcattc
     5281 acgccgcgca agatgcccat ccgcacccta cgcatgccgg gcgtgctctt aagatgtatt
     5341 acggcacaca agtccgctca gatccgccca ccttcatgat ctatgtcaat gaaccttcgt
     5401 tgatgcactt cacgtacatg cgttatttag agaatcaaat ccgcgctgag tatggtttct
     5461 taggaacccc cattcgtatt gtcctcaagg gcagacatga ataagaaaaa catcctcatt
     5521 acaggcgacg tcctcgcttt tgcgattcta aatattattg gctttgcaac tcacaaagag
     5581 actggcgttt ctttcctgcc gcgtatggca gttgcctttt tcccaattgt ttttgcatgg
     5641 tttgttctcg ctccatggtt tggtttgttc gatgagactg tcatcaataa ttcgaaaaat
     5701 ctttttagaa tcccactggc gtttctgttt gtagcccctc tggcagtgat cttgcgcgga
     5761 gcattgctca acgcgccagc gttgccgctt tttgcgttag tctttggcag tacgaatgcc
     5821 tttggtatgt tggtgtggcg ttggttatat ctcgttctgt ttgcgaaaaa agcgtaacat
     5881 ctaaaattca catcatttca aactattatc tctaaccatt tcagcaaaga cccggacaac
     5941 ctccgggtcg aattgtttat ccgcctgagc ttgaatataa gtcaacgcct cttcatctaa
     6001 aagtgctt
//
LOCUS       DAOQNJ010000092         5992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_7613_length_5992_cov_10.075459, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000092 DAOQNJ010000000
VERSION     DAOQNJ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7613_length_5992_cov_10.075459"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLG52_04135"
     CDS             <1..889
                     /locus_tag="PLG52_04135"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HNB40665.1"
                     /translation="GLISLFGVLIDLTLGNRYLSDSGMREPLAGAFASLLVGLPLWLL
                     NWRVAQSQALADSDLGDHARRSVIRKAYLYLELFATVIGGMVSAGFLVYNLINAVLGN
                     GISDFWTAVLNQLAVLIVFIVLLVYHLLALRADGTARADVLLEKQSKYKMVVFDADGK
                     FGNAVKSVFAKRAPQVPLTVVDANAELPADLNADAVILPGSLAVNTPEKVEAWLRSFG
                     GNKLIVSDEAAGVFWMNDFEQAADSAKSLAEGQDIRPQSSKRTASIWTVVAYVFAALF
                     ACQLLFGLLMFGVSMVTGF"
     gene            1061..2194
                     /gene="dnaN"
                     /locus_tag="PLG52_04140"
     CDS             1061..2194
                     /gene="dnaN"
                     /locus_tag="PLG52_04140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNB40666.1"
                     /translation="MKVTVLQENLARGLSIVSRAVSSRSTLPVLSNILIATDEGRLRL
                     SATNLELGITCWIPARIDQDGSTTVPSRTFSDLVNTLPGDQVQLTLDLKTQNLHVKGG
                     SSNNDIKCIDAQEFPPMPTPEMKDAVQLNVADFKEMIQQVVFASSTDEARPVLMGVLM
                     QVEKDKVTMAAADGFRLSVRKGQLAHSVKETLNIIIPSRALNELSRVAHDPEEPIYMV
                     VPKQRGQVLFRVKDVEVVSQLIDGTFPDYHQIIPRNYKSRTLVSTAALLKACKQAEIF
                     AREGSNVARLDIKQSNGDMQPSEVEISATSEETGKNETIVEATVDGSGVLIAFNVKFL
                     REALEVIKTPNVALETTAQNAPGVIRPVGDDNFLHVIMPMHLG"
     gene            2325..4700
                     /locus_tag="PLG52_04145"
     CDS             2325..4700
                     /locus_tag="PLG52_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNB40667.1"
                     /translation="MTLPRVSIDNVLRILLSLSQYPILSSRIRHQMRKTLYSRGIVRK
                     EVFEEEVKRKAVESQTLEGITDPLTEESNEVWLMRITRVKDSLTDFYFAYNLPYAEFE
                     DLVRKVLAERGSIDSDFVWVNPELAPQELLFEQAEMIESMPDADKEKYEARLQAIIAV
                     LIRTMISDQLKYIKIARQWFTVEDLREINRRKIGGGKIGGKAAGMLLAMRILKETAPV
                     QIRNRFRLPISYYLGSDVYYNFMSLNDLGGWNGQKYKTEEQMRADYPELCEDFYKGQF
                     PPEVIESLERVLEEAGQRPLIVRSSSLLEDNFGTSFAGKYESIFLPNQGDANECLKAL
                     TQAIAKIYASVMNPDALIYRHTKELADYDERIGILIQIVEGERAGRYYFPHGAGVAFS
                     RNLFRWSPQIKQDEGFLRLVTGLGTRAVDMLADDYPRLVALSHPRLHSSSDVRTIKRY
                     SQQHLDAIDIETNSFVTIPVRDALHTDYDPLRYIAQVEQDGYLAPIRSRLDSTDKLVI
                     TFDGLLSRTPFSKSMRAALTLLETHYGSPVDTEFTVEIVNPDSQPDVRITILQCRPQS
                     HIHEANEVQIPSDLETQDIVFSSQTMVPQGVVENIGYVLFVPSEGYFSLETQAERTQL
                     ERAIGQLNAALKGQTFIAVGPGRWGTSTPDLGVHVAYSDIYNSRALVELAGEEIGASP
                     EPSFGTHFFQDLMEANIYPLGVFVDEEGTIFKRDFFYSTPNRLSEFISIENPRVVAAL
                     KLIAVSDFRPNTHMNLIMEASKSRAVAFLVGKATPIESDDAIVGTAPTSLE"
     gene            complement(4758..5678)
                     /locus_tag="PLG52_04150"
     CDS             complement(4758..5678)
                     /locus_tag="PLG52_04150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNB40668.1"
                     /translation="MIEKKNIYWFLGITVLLSWPLFLSTLFLGNMDAQTKQLTTQGLW
                     AAAMWGPGIAAIITTLFIAKVPFNSLRLNTIGPKRFYLWAWFLPTVFTVLSGLFTILF
                     GIGKLDLSFSMIRTALESTPGGSEIPASVVVLSQSLLALTLAPFLNMLFTMGEELGWR
                     GFLLPRLLPLGQWKAVLWSGLIWGLWHAPVIVQGHNYPGYPILGVFMMIVFCFLLGTI
                     IAWLYINTKSPWVAALAHGAVNAIAGLPVLFLAPGFNMAFGGTLAAPTAWIGMALFIA
                     WLVWTKRFPVQDQTGDAQSTSEVDGSEITK"
     gene            complement(5690..5929)
                     /locus_tag="PLG52_04155"
     CDS             complement(5690..5929)
                     /locus_tag="PLG52_04155"
                     /inference="COORDINATES: protein motif:HMM:NF025156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HNB40669.1"
                     /translation="MNNNELSKWEYRVQTVGNFWSGVKATELEELLNAWGEEGWEVVS
                     THVLENQNKINVIAKRPLTRETIRQRSMPNYQYEK"
BASE COUNT         1517 a         1386 c         1438 g         1651 t
ORIGIN      
        1 cggcttgatc tctctgtttg gcgttttaat cgatttgacc ttgggcaatc gctacttgag
       61 cgactctgga atgagagagc ctttggcggg tgcgttcgct tcgttgttag tgggcttacc
      121 tttatggctg ctgaactggc gtgtggcgca atctcaagcg ttggcagata gtgacctggg
      181 tgaccatgct cgccgttcag tgattcgcaa agcctatctc tatctcgaat tgtttgctac
      241 ggtcattggt gggatggtct cggctggttt tttggtctac aacctgatca acgctgtttt
      301 gggaaatggc atctccgatt tttggacggc ggtgctcaac caactcgcgg tgttgattgt
      361 gttcattgtt ttgctggtct atcacttgct tgctttgcga gccgatggca ctgcacgagc
      421 tgatgtattg ctggaaaaac agtcgaagta taagatggtg gtttttgatg cagatggcaa
      481 gtttggaaat gcagttaagt cagtatttgc caaacgtgcg cctcaagtgc cattgacggt
      541 ggtggatgcg aacgcagagt tgcccgctga cttgaatgca gatgctgtga ttttgcccgg
      601 ctctttggct gtgaatacgc cagaaaaggt tgaggcgtgg ttaaggtcct ttggcggtaa
      661 caagctcatc gtcagtgatg aggcggcggg cgtattttgg atgaatgatt tcgagcaggc
      721 agcggattcg gcgaagtctc tggctgaagg gcaggatatc cgcccgcagt cttccaaacg
      781 cactgcctct atttggacag ttgtagccta tgttttcgca gccttgtttg cgtgtcagtt
      841 gttgtttggg ttgctgatgt ttggggtctc gatggtgaca ggcttctagt aaagaagtag
      901 caaaaaattt ctacccgaag gagtgacatg gcttgtaaac gctgtgctat tccttcggga
      961 tttgttttaa ttcgcgattg ttatctataa tccttccatc cctttgggat tggatgatat
     1021 acttttgaaa attctgacta attattaagc gaggcgcacc atgaaagtaa ctgtccttca
     1081 agagaatctt gcccgtggtc ttagtattgt ttcgcgcgca gtttcctcgc gcagcacact
     1141 ccctgttctt tcgaatattt tgatcgcgac cgacgaaggt cgtttgcgac tttcagccac
     1201 caaccttgag ttgggcatta cgtgttggat tcccgctcgc attgatcagg atggttcaac
     1261 gaccgttccc tcgcgcacat tttcagattt ggtgaatacc ttgccgggcg atcaggtgca
     1321 gttgacgctt gatctgaaaa cccagaatct gcatgtaaaa ggcggctcct cgaacaacga
     1381 tatcaagtgt atcgacgcac aggaattccc gcccatgcct acgccggaga tgaaagatgc
     1441 cgtgcagttg aacgtggcag atttcaaaga aatgatccaa caggtggttt ttgcttcttc
     1501 caccgatgaa gctcgcccgg tattgatggg tgtgttgatg caggttgaaa aagacaaggt
     1561 caccatggcg gcagcggacg gctttcgcct ttcggtgcgc aaaggtcagc ttgcgcactc
     1621 tgtgaaagag acgctcaata tcatcatccc ttcacgtgcg ttgaacgaac tttcgcgtgt
     1681 ggcgcatgac cccgaagaac ccatttatat ggttgtccca aaacagcgcg gacaagtcct
     1741 tttccgtgtg aaagatgtgg aagtagtctc gcagttgatc gatgggacct tcccagatta
     1801 tcaccagatc attccgcgca attacaagtc tcgtaccttg gtttccactg cggctttgct
     1861 caaagcctgc aaacaagcgg agatctttgc ccgtgaaggt tcgaatgtgg cgcgtttgga
     1921 tatcaaacaa tcgaatggcg atatgcagcc cagcgaagtg gaaatttctg cgacctcgga
     1981 agaaactggt aagaatgaaa ccatcgttga agccaccgtg gatggtagcg gtgttttgat
     2041 tgcgttcaac gtcaagttct tgcgtgaggc gctcgaagtc atcaagaccc cgaatgtggc
     2101 gctggagacc acggctcaga atgcgccggg agtcatccgc ccagttggtg atgacaactt
     2161 cttgcacgtc attatgccga tgcatttggg ataggcttac aaacagaata cgaatccgcc
     2221 agagacctat ctggcggatt ttttgttata aaatcggaca atgaaccgca gatgatcgac
     2281 ctgcgttttg tctctatcgt cagttgtcca tcaaaggtga attcatgact ctcccacgcg
     2341 tttccattga taacgtactt cgcatcttgc tttctttgag ccaatatccc attctcagca
     2401 gtcgcattcg ccatcaaatg cggaagacgc tgtattcacg cggtattgtt cgaaaagagg
     2461 tttttgaaga agaagtaaaa cgcaaagccg ttgaatctca gacgcttgag ggcattaccg
     2521 acccgttgac ggaagagtcg aatgaagttt ggttgatgcg tatcacccgt gtaaaagact
     2581 cgctcaccga tttttatttt gcatacaacc tgccatacgc tgaatttgaa gatttagtcc
     2641 gcaaggtgtt ggcagaacgc ggcagtattg actcagattt tgtttgggtc aacccagaac
     2701 tagcgccaca ggaattgctt tttgaacagg cagagatgat cgaatccatg ccagatgcag
     2761 acaaggaaaa gtacgaagcg cgtctgcaag ccattattgc ggtattgatc cgtactatga
     2821 tcagcgatca attgaaatac atcaagatcg cgcgtcagtg gtttactgtg gaagatctgc
     2881 gtgagattaa tcgtcgcaag atcggcggcg gaaagatcgg cggtaaagcg gcgggcatgt
     2941 tgcttgccat gcgcatccta aaagagaccg cgccggtaca gatccgcaac cgtttccgtt
     3001 tgcccatttc ttattatctt ggctccgatg tgtactacaa cttcatgtcg ctcaatgacc
     3061 ttggtggttg gaatgggcag aaatataaaa ccgaagaaca gatgcgtgca gattaccccg
     3121 agctgtgcga agatttttac aagggacaat ttccacctga agtgatcgaa agccttgagc
     3181 gcgttttaga agaggcaggt caacgcccgc tgatcgtgcg ctcctccagt ttgcttgaag
     3241 ataattttgg cacatcgttt gccggaaaat acgaaagcat tttcttgccc aatcaagggg
     3301 atgctaatga atgcctcaag gcactcacgc aagctattgc caagatctat gccagtgtaa
     3361 tgaaccccga tgcgctgatt tatcgtcaca ccaaagaact tgctgattat gatgaacgca
     3421 tcggtattct gattcaaata gttgaaggcg aacgagctgg tagatattac tttccccatg
     3481 gggctggtgt ggcatttagc cgaaaccttt tccgctggtc tccgcagatc aaacaggatg
     3541 aaggcttctt gcgtcttgtt actggtttag gtacccgcgc cgtcgatatg cttgcagatg
     3601 attatccgcg attagtagcg ctcagtcatc cgcgtttgca ttcgtcttca gatgtccgca
     3661 ccatcaaacg ctattcgcaa cagcaccttg atgcaattga cattgaaacg aattcttttg
     3721 taaccatccc cgtgcgcgat gctctccaca cggattatga tccgctgcgc tatatcgccc
     3781 aagtggaaca agacggatac ctcgctccga tccgttcgcg cctcgactct accgataaac
     3841 tggttattac cttcgacggc ttactctcgc ggacgccttt ctcaaagtcc atgcgagcgg
     3901 cattgacttt gcttgaaaca cactatggct cgcccgttga tacagagttc acggttgaaa
     3961 tagtaaaccc tgatagtcag ccagatgtcc gcattacgat tttgcagtgt cgtccgcaaa
     4021 gtcatattca cgaagcgaat gaagtacaga ttccaagtga ccttgaaaca caagacatcg
     4081 ttttctcgtc acaaaccatg gtgccacaag gggttgtgga aaatatcggg tatgttttgt
     4141 ttgttccttc agaaggttat ttcagccttg aaacacaagc agagcgtacc caactggaac
     4201 gcgccattgg tcaactcaat gcggcgttga aaggacaaac ctttatcgca gtaggacctg
     4261 gacgctgggg aacatcgaca ccggatttgg gtgtgcatgt ggcgtacagc gatatctata
     4321 actcgcgtgc cttggttgaa ttggcgggag aagagatcgg agcatctcct gaaccttctt
     4381 tcggcaccca tttcttccaa gaccttatgg aggcgaatat ctatccgctt ggtgtatttg
     4441 ttgatgaaga aggaactatt ttcaagcgtg actttttcta tagcacccca aatcgacttt
     4501 ctgaatttat ctctattgaa aatccacgag ttgtcgctgc tttgaaatta attgctgttt
     4561 cagactttcg tccaaatact cacatgaatt tgatcatgga agcaagcaaa agtagagctg
     4621 tcgcttttct agttgggaaa gctactccaa ttgaaagtga tgacgcaatt gttggaacag
     4681 caccaacaag tttggaataa aaaaatgggc gactcatcgt cgcccatttt tttataatta
     4741 caaaaagaac aaatgttcta ttttgtaatt tcactcccat caacttctga tgtcgattgt
     4801 gcatcgcctg tttggtcttg tactgggaaa cgtttcgtcc acaccaacca tgcaatgaac
     4861 aaagccatgc caatccatgc agttggtgct gcaagtgttc caccgaatgc catattgaaa
     4921 ccaggtgcca ggaacaatac aggcaaccct gcaattgcat tgactgctcc atgtgcaagc
     4981 gctgcaaccc atgggctttt ggtattgata tacaaccatg caatgatcgt cccaagtaag
     5041 aaacaaaata cgatcatcat gaacacaccc agtatcggat acccaggata gttatgtcct
     5101 tgtacgatca caggtgcatg ccacaagccc catatcaagc cactccataa aacggctttc
     5161 cattgtccga gtggcaacaa tcgcggaagt aaaaatccac gccagcctaa ttcttctccc
     5221 atcgtgaata gcatgtttaa aaatggtgca agtgttaatg ccagcaatga ttgagataag
     5281 acaacaacgc ttgctggaat ttcacttcca cccggcgtag actctaatgc agttcgtatc
     5341 atgctgaagc tcaaatccaa tttgcctatt ccaaatagaa tagtgaagag tccactcagc
     5401 acagtgaata cagtgggcag aaaccatgcc caaagataaa agcgttttgg accaatcgta
     5461 ttcaaacgca aactattgaa tggtactttg gcaatgaaca aagtagtgat gatggctgca
     5521 attcctggac cccacattgc agcagcccat aaaccttgag tggttagttg tttagtttgg
     5581 gcatccatat ttccaaggaa caatgttgaa aggaataacg gccatgaaag caacactgta
     5641 attcctagaa accaatagat gtttttcttt tcgatcattt tatatctcct tacttttcgt
     5701 attgataatt cggcatcgaa cgttgacgga ttgtttcgcg agtcaacgga cgtttcgcga
     5761 tcacattgat tttgttctgg ttttcaagca catgtgtgga tacaacttcc cagccttctt
     5821 caccccaggc gttcaatagt tcttccaact cggtggcttt caccccagac caaaaattcc
     5881 caacggtttg tacgcgatat tcccatttgg ataattcatt attgttcatt ggaacccttt
     5941 cgtttcacgt gaaacaaaac gtgcgtgtct gtaccagaca cgcacgtttt gt
//
LOCUS       DAOQNJ010000093         5963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_7671_length_5963_cov_9.521158, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000093 DAOQNJ010000000
VERSION     DAOQNJ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7671_length_5963_cov_9.521158"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLG52_04160"
     CDS             complement(<1..896)
                     /locus_tag="PLG52_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40670.1"
                     /translation="MVRKLSVVLLILFQISCSAATPEPTATFTPLPPTATVTPTPAPT
                     ATQLPASPMPDAITYEGNGFRFAVPDTLDVDMQKNAVGIYDKSGTLIISFTRVPYDES
                     TDTLEDVINDYLAEISTRGGGKFTQSEATSITVAGAEGLSVELSGTLFERPIEGRAIA
                     VVPDKNSVLFGMGVSNLVSDETLWQSTGSLIFQGLLDSLEFVDVQIAAQCTVSTDKSY
                     GYTETNPIKVGGDFIDGPARERAYLDNLLGPNGEALSYEREGSINSGDTILDIYRVTG
                     NGLNVTLYLDEYTYSPLLAPVG"
     gene            1094..1924
                     /locus_tag="PLG52_04165"
     CDS             1094..1924
                     /locus_tag="PLG52_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB40671.1"
                     /translation="MHRERVSENVFWFQSEVYAQVTAGVIIGPQWAVVIDTLALPEET
                     LSMREFIEHELGVQVRYVINTHYHADHCWGNCFFPGAVVIGHSKCRQYLVEDGAESLE
                     NAQKQNPNLRQVKIVPPHITFETGEMTLRVGKKNLIISTSPGHSDDGISVLVEEDRVL
                     FAGDAFMPLPFIVDGDADDMLNSIKKIGKMGLENIIQGHGDIILRGEIDAAVRENVNY
                     LNNIKKAVKSGGKRKNADEYLEEIAIEDCGKSRVYLGGLAETLHRRNLRALHRQMFGE
                     "
     gene            2018..2470
                     /locus_tag="PLG52_04170"
     CDS             2018..2470
                     /locus_tag="PLG52_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNB40672.1"
                     /translation="MKFLLERSRYLTLIGIISLLIASFAAFAWGTIKTVTTTLLVIET
                     LGKDSSITIELIELIDIFLIATTILIFSASLYELFIGKLDMPEWMLAHDLYELKAKLS
                     SMIVLVMAVKYLQKLIEIKDGTELLQRGIAVAIVSAALIAFGYFGKKD"
     gene            2499..3053
                     /locus_tag="PLG52_04175"
     CDS             2499..3053
                     /locus_tag="PLG52_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40673.1"
                     /translation="MTGLPPFQQGGLDSLCGLYSIINAERIINHSTDDESQALFDGLI
                     RYLSKRGLLGKFLVGGILHPQMLLILNKVVGKKRISNVNIPWRGVPNPDLTTFWKSMQ
                     SFLDGTQGRAIILGIMGYHEHWTVIEKITNRSILLYDSALIKRLPRNFCTTVYATGQR
                     KHVLLPAQTYFLSNEVFDITQYDT"
     gene            3090..3452
                     /locus_tag="PLG52_04180"
     CDS             3090..3452
                     /locus_tag="PLG52_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNB40674.1"
                     /translation="MTLDLAPFEKEEYCYLTTKGRKTGTPHEIEIWFTIHENAVYLLS
                     GGMDKSDWVKNLLQEPNVTLRIAGQTFSAFASILEDKELEPMIRMAMAIKYDEWEGQD
                     PSEWARTALVVKFIPQSL"
     gene            3714..>4137
                     /locus_tag="PLG52_04185"
     CDS             3714..>4137
                     /locus_tag="PLG52_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40675.1"
                     /translation="MNTIRKSFSVIFSFLCIGLLIACIPYTPPPPPPPPGVALTLTVQ
                     AIQLGFFTQTAQALQSQPIVIVVTATPDPNNLAPTTDPNAAPAATTSSAVTITVSQNT
                     NCRLGPSQDFDEIGSLGPGQVAEVVGKDTYDNYWVIKLP"
     assembly_gap    4138..4237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4238..4684
                     /locus_tag="PLG52_04190"
     CDS             <4238..4684
                     /locus_tag="PLG52_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40676.1"
                     /translation="AEVVGKDTYDNYWVIKLPNNPAITCWLWGQYATVTGDISTVADS
                     ATPTAISNGAPNAPSLLDAQVKCTKLSGKDYQYKITLKWADNSSDENKFVINTSTGKT
                     FSTPPNKTSFSFEEGLPSETNLSATITAANEKGESQPVGTGAFKCP"
     gene            4799..5698
                     /locus_tag="PLG52_04195"
     CDS             4799..5698
                     /locus_tag="PLG52_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40677.1"
                     /translation="MNAARKSLILLVVLILSTLACGLENIQVVEAPNLEATITAQAAL
                     LQQGGQPAQDPAQQVIVVVTATPEGGVAPTEQVQAAQVLPPATGDVTVTVTTATNCRT
                     GPGQNFKIVYGMPIGQVAKVVAKNSYSGYWIIEIPGQTGQNCWLWGQYAVINGDTSTL
                     KEVVTPTSPAPTKTNTPTPTATATATTGVVFPNAPSGLTASISCAAGPGGTQTTVSGN
                     ISWVDNSTNEQVFVSSVNGVSSHTLGVNSTSDSFSTALETSALQAVAPGQQFTVSVYA
                     CSGNTAGTCGNTVTITVTGAGCP"
     gene            5779..>5963
                     /locus_tag="PLG52_04200"
     CDS             5779..>5963
                     /locus_tag="PLG52_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40678.1"
                     /translation="MNKRNPYFFLFLILAFLLSACNLQLVESPQDSDSANLELTAIVQ
                     QMLIDQAAQQGNVVEITA"
BASE COUNT         1612 a         1554 c         1286 g         1411 t
ORIGIN      
        1 aaccccaccg gtgcaaggag aggcgaatat gtgtattcat ccaaatacaa ggttacattc
       61 aaaccgttgc ctgtaacacg ataaatatcc aagatcgtgt caccgctatt tatagagcct
      121 tcacgttcat aagacaaagc ctcaccattc ggacccaaca aattatcgag ataggctcgt
      181 tcacgagcag gtccatcgat aaaatcgcct ccgaccttga tcgggttggt ttctgtatac
      241 ccatagcttt tatctgtgct gacagtacac tgcgcagcga tttgcacatc gacaaattcc
      301 agtgaatcca atagcccctg aaagatcaat gaaccagtgc tttgccatag cgtctcatca
      361 ctgactaaat ttgagactcc cataccaaat agcaccgaat tcttgtcagg caccaccgca
      421 atggctctgc cttcaatggg tctctcaaaa agagtgccgc ttagttccac agaaagcccc
      481 tctgccccag ccactgtgat cgaagtcgcc tcgctttggg tgaacttgcc gccaccacgc
      541 gtagaaatct ctgccagata atcattgatc acatcttcca atgtatcggt agattcatca
      601 tacggcactc gggtaaaaga gatgatcaaa gtgccagatt tatcataaat ccccacggca
      661 ttcttttgca tatccacatc taaggtatca ggtaccgcaa agcgaaaccc gttaccttcg
      721 tatgtaatcg catcaggcat agggctagcc ggaagctgtg tagcggtcgg agcaggcgta
      781 ggtgtgacag tagcggtggg cggcaatggg gtaaaagtcg ccgttggctc gggggtagcc
      841 gcactacatg agatttggaa caggatcaac aaaacaacag aaagttttcg aaccatctag
      901 aatcctcctt tttgggtgaa gggaaaggca caagtcttgt cagaatataa ctcaaatctg
      961 aagaaatctc catatttgtc tatacatctt gaaattttta agattgtatg ctattaacaa
     1021 cgaatactaa acacaaatgg cttcgctcca gtatgggata gcaaaccggc gaaccagtga
     1081 agaaggtaaa actatgcacc gcgaacgagt ttctgaaaac gtattttggt tccaaagcga
     1141 agtctacgcg caggtcactg cgggtgtcat tattggtccg caatgggcag ttgtcatcga
     1201 taccctcgct ctccccgaag agaccctctc catgcgcgaa tttatcgagc acgaattggg
     1261 cgtacaggtc cggtatgtga tcaacaccca ttatcacgcc gaccactgct ggggtaactg
     1321 tttcttcccc ggcgccgttg tgatcggtca ttcgaaatgc cgccaatatc tggtggaaga
     1381 tggcgctgaa tcattggaaa atgctcaaaa gcaaaaccct aatctgcgtc aggttaagat
     1441 cgtgccgccg cacatcacct ttgaaaccgg tgaaatgacc ctacgtgtgg gtaagaaaaa
     1501 tctcatcatc tccacttccc ccggtcacag tgacgatggc atttccgtcc tcgtcgaaga
     1561 agaccgtgtg ctcttcgccg gtgatgcctt tatgccgttg cccttcattg tagatggcga
     1621 tgccgatgac atgttgaact ccatcaagaa gatcggcaag atgggcttgg aaaatatcat
     1681 ccaaggtcat ggcgatatca tcctgcgagg tgagatcgac gccgccgtac gtgagaatgt
     1741 caactacctc aacaatatca aaaaagcagt caagtcgggt ggcaaacgca agaatgccga
     1801 tgaatatctc gaagagatcg ccattgaaga ttgtggcaag agccgtgtct atctcggcgg
     1861 gctggcagag accctgcaca ggcgcaacct gcgagcctta caccgccaaa tgttcggaga
     1921 gtaaacgaat acactagatg ttgcagatgc aagccgggtc tgcaacatct ttgttagtgg
     1981 ctagagcctt atacattatg acaagaggat taacaacatg aaatttttac tggaacgttc
     2041 acgctaccta actttgatcg gcatcatctc tctgttgatc gcttctttcg ccgcctttgc
     2101 ctggggcacc atcaaaacag tgactactac cctgttagta atcgaaaccc tcggcaaaga
     2161 cagttccatc accatcgagt tgatcgaact gatcgatatt ttcctgatcg ccaccaccat
     2221 tctgattttt tctgccagcc tgtacgaact cttcatcggc aaattagaca tgcccgaatg
     2281 gatgttggca catgacctgt acgaactcaa agccaaactc agcagcatga tcgtgctggt
     2341 aatggcagtc aagtatttgc aaaaactgat cgaaatcaaa gatggaactg aactcctcca
     2401 acgcggcatt gccgttgcca tagtctctgc ggcgctgatc gccttcggat atttcggcaa
     2461 aaaggactga tggcaccatg agaaggtcaa gccgcttaat gacgggactt ccgccctttc
     2521 aacaaggcgg cttggactca ctttgcggct tgtacagtat tatcaacgcc gaacgcatca
     2581 ttaaccattc caccgatgat gaatcgcaag ccttgtttga cggactgatc cgctaccttt
     2641 ccaagcgcgg gttacttgga aaattcctcg tcggcggaat cctccaccca cagatgttgc
     2701 tgattctgaa caaggtcgta ggaaagaagc gtatctccaa cgtaaatatt ccctggcgcg
     2761 gcgttcccaa cccagacctc accaccttct ggaagtcgat gcaatccttt ttagatggca
     2821 cccaaggtcg tgccattatc cttggcatca tggggtatca cgagcattgg accgtcatcg
     2881 aaaaaattac caaccgttct attctgctat acgactcggc actcatcaaa cgcttaccac
     2941 gcaacttctg caccaccgtc tacgccacag ggcaacgcaa gcacgttcta ttaccggcac
     3001 aaacctattt tctctccaac gaagtttttg atattacaca atacgatacc tagacttagc
     3061 ccacctactg aatgattttg gagacatata tgaccctaga ccttgctcca tttgaaaaag
     3121 aagaatactg ttacctcacc acaaagggac ggaaaaccgg aacgcctcat gagatcgaaa
     3181 tctggttcac catccatgag aacgcggtct accttctctc tggcggcatg gacaaatcgg
     3241 attgggtcaa aaacttgctg caagaaccca acgtcactct tcgcattgcg ggtcaaacct
     3301 tctctgcctt cgccagtata ttggaagaca aagaactaga acccatgatc cgcatggcaa
     3361 tggcgatcaa gtatgatgag tgggaagggc aagaccctag tgaatgggca cggacagcat
     3421 tggtagtgaa gttcattccc caaagtttat aaagtcattt gtgataaata acatattacg
     3481 agtaaattac agtacttaag aacaccttac gtaaatgaaa agtccagtaa aatgagggaa
     3541 attatctaat tggggacatt tagttttgcc aattttattt catgctcttt gccatgtgcc
     3601 aagctccgca caggtaaagc ctttcataac gccggagtga aagggatgac tatttggcaa
     3661 atatacaact acctccattt tagggaattc ccatttcgta caggagctgc tccatgaaca
     3721 ctattcgtaa gtcattttct gtaattttct cctttttatg tattggttta cttattgctt
     3781 gtattccata tacccctccc cctcccccgc ctccgcctgg ggttgcgttg acgctcaccg
     3841 tgcaagccat tcaactgggc ttcttcactc aaactgccca ggcattacaa tctcaaccca
     3901 ttgttattgt tgtcacggct acgcctgacc cgaacaatct tgctccaaca acagacccta
     3961 atgcagcccc agcggctact acatccagcg cggtgacgat caccgtcagc cagaacacca
     4021 actgtcgtct cggtccgagc caggactttg acgagattgg ctctttggga cctggtcagg
     4081 tcgcagaggt agtcggtaag gatacatacg acaactattg ggtcatcaaa cttccaannn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca gaggtagtcg gtaaggatac
     4261 atacgacaac tattgggtca tcaaacttcc aaacaaccct gcgatcacat gctggttatg
     4321 gggacaatat gccacagtga cgggtgatat ctcaaccgtt gcagatagtg ccacgcctac
     4381 tgctatttcc aatggtgctc ccaatgcccc atctctttta gacgcccaag tgaaatgcac
     4441 caaactgagc ggtaaagatt atcaatataa gatcaccctc aaatgggccg ataactcttc
     4501 cgacgaaaat aaattcgtaa tcaatacgtc caccgggaaa accttctcaa caccacccaa
     4561 taaaacctct ttcagttttg aagaaggtct tccttccgag acaaaccttt ctgccacaat
     4621 aactgccgcc aatgaaaagg gcgaatctca gccggtagga actggcgcct tcaaatgccc
     4681 ataaaagggc atccctacta tctagcctaa gtaaaagaaa attcaccaca gagaagattt
     4741 tattttctct gtggtgaaaa tctattcagc aattatctct gaacgaggag aattaagaat
     4801 gaacgccgcc cgaaaatctc ttatcttgct agttgtcctc atcctatcca cgctggcttg
     4861 cggactggaa aatattcaag tcgttgaagc gccaaacctg gaggcgacca tcaccgcaca
     4921 agccgccttg ttgcaacaag gcgggcaacc tgctcaagat cctgctcaac aagtcatcgt
     4981 ggttgtgacc gccacacccg aaggtggagt tgctcccact gaacaggttc aagccgctca
     5041 ggtgctgccc cccgccaccg gcgatgtgac cgtcactgtc accacggcaa ccaactgccg
     5101 caccggtcct ggacagaatt ttaagatcgt ctatggcatg ccgatcggac aggtggcaaa
     5161 ggttgtggca aaaaattcat actctggcta ttggatcatt gagatccccg ggcagaccgg
     5221 gcagaactgc tggttgtggg gacaatacgc cgtcatcaat ggcgatacct ccacgttgaa
     5281 ggaagtggtc acgcctacat cgcccgcgcc gacaaagacc aacaccccca ccccgacagc
     5341 cacggctact gccaccaccg gagtggtctt ccccaatgca ccctccggct tgaccgctag
     5401 catcagttgc gctgccggac ccggcggtac ccagacaacc gttagtggaa acatatcatg
     5461 ggtagataac tccaccaatg agcaagtctt tgtttcgagt gttaacggcg tctcctcaca
     5521 taccttgggc gtcaactcga ctagtgatag tttcagcacc gccctagaga cgagcgccct
     5581 tcaagctgtc gctccaggtc aacaatttac ggtctcagtc tatgcctgta gcggcaacac
     5641 agccgggact tgtggaaaca ctgtcaccat cactgtaacc ggcgctggct gcccataata
     5701 aacaataaag ctcacagtcc ttgtgaaaaa ttcgcaagga ctgtgagtct ttaatatcca
     5761 tagggcaaag atatcatcat gaacaaaaga aacccatatt tttttctctt tttgatattg
     5821 gcatttttat tatccgcctg taacttgcaa ttggtagaaa gcccgcagga ctcagatagc
     5881 gccaaccttg aattgaccgc catcgtccag cagatgttga tcgaccaagc agcgcagcaa
     5941 gggaacgtgg tagagatcac tgc
//
LOCUS       DAOQNJ010000094         5817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_7988_length_5817_cov_9.116973, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000094 DAOQNJ010000000
VERSION     DAOQNJ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7988_length_5817_cov_9.116973"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..683)
                     /gene="rpiB"
                     /locus_tag="PLG52_04205"
     CDS             complement(225..683)
                     /gene="rpiB"
                     /locus_tag="PLG52_04205"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNB40679.1"
                     /translation="MKVAVGCDHGGFPLKDVVLEAVKAMGHEVIDVGTFNAEAVDFPD
                     FTKKVGEKVQSGEAERGILICGSGIGAAIAANKMKGIFASICHDTYSAAQGVEHDAMN
                     VLCMGGRVIGPELVKVIVPAFLNAEYLGDKTGGERFARRVGKIRKMEENQ"
     gene            complement(763..2733)
                     /gene="tkt"
                     /locus_tag="PLG52_04210"
     CDS             complement(763..2733)
                     /gene="tkt"
                     /locus_tag="PLG52_04210"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNB40680.1"
                     /translation="MTNLEFQNKAINTLRFLSADGVQKANSGHPGLPMGAAAMAFTIW
                     TRHLRHSPRNPKWMGRDRFILSGGHGSMLLYSLLHLTGYDLSLEDLQSFRQWGSKTPG
                     HPEYGWTPGVEVTTGPLGAGLSTGVGMAIAGAHLAATFNKPGHELIDSYIYAIVTDGD
                     LMEGVASEAASLAGHLSLGKLIYLYDDNKISIDGSTDLSFTEDRAKRFEAYGWHIQRV
                     EDGNDVEAIDSAIKAAKTDPRPSLILCRTIIGFGAPNRQGTQKAHGEPLGDEELNAAK
                     DNLGWPKEPRFLIPDDVLEFYRKAVDRGRELEADWKMRREAYARLFPDLGTEFNRRIL
                     MNKLPEGWESALPTFPADPKGMATRAASGKVINALAAKLPELIGGSADLAPSNNTKID
                     GSPAFQKDSPAGRNFHFGVREHGMGSVLNGMAIFGGVIPYGATFLVFADYMRASVRLA
                     ALSHYPTIFIFTHDSVGLGEDGPTHQPVEHLASLRIIPNLVVLRPADANETAQAWKAA
                     IARRDGPTVLALTRQNVPTMDGPALVEKGAYVLKDFGTPEIILMASGSEVGLVMDAAQ
                     KLADEGKGVRVVSFPSWELFEKQDEAYRESVLPKSIQKRLAVEAGSSIGWERYAKSVI
                     GIDRFGASAPAKTIFEKLGFTVENVVAKAKEL"
     gene            complement(2755..3513)
                     /locus_tag="PLG52_04215"
     CDS             complement(2755..3513)
                     /locus_tag="PLG52_04215"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HNB40681.1"
                     /translation="MLFTDMVFVGIDAASSHKSYTYAALDKGMNLVALADGELDDVTT
                     FLGGQSSAIVAINAPSGVNRGLVREKKKDMFKSVKPRAAGFRVAELELRDRGITVSGT
                     PSTISACSAWVQSGFALYRKLEKMGFKKYPTENTPYQILESQPHAAFCVMAEAIPQPK
                     PSLEGKIQRQLLLYEAGVRIKDPMDFFEEITRHKMLKGNWPLDMLYQPEQLDALVAAY
                     TAWLSIHKPEKVSLIGDSTEGQIALPEKNLKEKY"
     gene            3581..4123
                     /locus_tag="PLG52_04220"
     CDS             3581..4123
                     /locus_tag="PLG52_04220"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB40682.1"
                     /translation="MNRTQRIILYILLLIAGASWIVISGQPTGTTTVAAAAPQAGFTA
                     PDFTLKTAEGETYTLSELKGQAVLINVWATWCPPCKAEMPAIEKMYNEYKGQGFNVLA
                     INSTFQDDPLQIKPFTEEYSLTFPILLDETSDISRDYQVRSLPSSYFVNRYGIITEVV
                     IGGPMSEALLRTKIEEALKP"
     gene            4120..4848
                     /locus_tag="PLG52_04225"
     CDS             4120..4848
                     /locus_tag="PLG52_04225"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNB40683.1"
                     /translation="MIETFQNLFAPPRHMILLVIAAWLGLALAERRTERHGISKEDLN
                     NITFYGLIAFVIGGRISYIIQNISAFTKSPLGIVSINPNLFDPIGAIAVAFIVLIVYG
                     QRKQLNIWNIFDSLTLFFAVLAIGLGLSHLAAGSAFGLPTDLPWGINLWNATRHPTQI
                     YDTLASSIILILLWLFKPSPRPGITFLTYAAFVALAQLILQAFRANYTIIFSGLRQEQ
                     VVAWVGLLVCFILIEVRLKSQEKQ"
     gene            complement(4892..5455)
                     /gene="raiA"
                     /locus_tag="PLG52_04230"
     CDS             complement(4892..5455)
                     /gene="raiA"
                     /locus_tag="PLG52_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNB40684.1"
                     /translation="MSNKVEVQTRHIRLTERIEEYVSKKAGNLDHYLPAIEDARVELS
                     HHQAARDVNDRNVAQITVFGKGFTLRSEERADEALAAFDKALDNMQRQIERHKGKKYH
                     GRGDGRSAAEAVDDIIDDETGQLSPLFAKRKKFVMYPMNEEEALVQMRNLGHDNFFVF
                     YNAETSKINVLYRRRNGSYGLIEPELG"
     gene            complement(5497..>5817)
                     /locus_tag="PLG52_04235"
     CDS             complement(5497..>5817)
                     /locus_tag="PLG52_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNB40685.1"
                     /translation="RGYNQVAMIAKPLAMGLGLQYSPHALARRKETRSQVGLSREERQ
                     KNVHDAFQASGVQGKIALIMDDVSTTGSTLSSGAEALYAAGAKEVYALTVARALSHHD
                     LRHV"
BASE COUNT         1527 a         1533 c         1247 g         1510 t
ORIGIN      
        1 cgtttttgca atggcatttt ggaaaatagt cgggttggag gtcacaccgc gaatgtcgcc
       61 acgattgatc atggctttga gttcgccgtt gaccaaaagt ttacgttgga tgttatcgta
      121 ccaaagcgat tgcccaaggg cagttaattt cttaattgat tcagacataa taaaaaatgc
      181 tccttaaatt aattaaatag taactggtaa ttaccaatta ctatttactg gttctcttcc
      241 attttcctaa tctttcccac ccggcgcgca aagcgttcac cacccgtttt gtcccctaaa
      301 tattctgcat tcaaaaatgc aggcacaata acttttacca gttctggtcc gatcacacgc
      361 ccgcccatac aaagcacatt catggcatcg tgctccacgc cttgtgcggc tgaatacgtg
      421 tcgtggcaga tcgaagcgaa aatgcctttc attttattgg cagcaatcgc ggcaccaatg
      481 cccgaaccgc aaatcaaaat tccgcgctcg gcttcgccgc tttgcacttt ctcaccaact
      541 tttttcgtga aatccggaaa gtccacagct tcagcattga acgtccccac atcgatcact
      601 tcatgtccca tcgccttcac agcttcaaga acaacatcct taagcggaaa tccgccgtgg
      661 tcacaaccta ctgcaacttt cataattctc caaaagtaag ggcgacccat cttggcagag
      721 tcaggtctct gaccattccg cgcgggtcgc ccctacgatc cattaaagtt ctttcgcctt
      781 cgctacaaca ttctcaacag taaagcccag cttttcaaag atcgtcttcg ccggagcaga
      841 tgcaccgaag cggtcaatac caatgacgga cttcgcgtat cgttcccagc cgatacttga
      901 tcccgcttcg acagcgagtc tcttctgaat gcttttcggc aacacagact cgcgataggc
      961 ttcatcctgt ttctcgaaca attcccaact cgggaaagac actacacgca cacccttgcc
     1021 ttcgtccgct agtttttgtg cggcatccat taccagccca acttctgagc cagatgccat
     1081 gagaataatt tcaggggtgc caaagtcttt caagacatag gcgccttttt caaccaatgc
     1141 aggaccatcc attgtaggaa cattttggcg ggtcaatgcc aagacagtag gaccatcacg
     1201 gcgagcaatg gcagctttcc acgcttgagc cgtctcattg gcatccgctg ggcgaagcac
     1261 aacaagattc ggaataatac gcaaagaagc caaatgctcc acaggttgat gtgtcggtcc
     1321 atcttcgccc aaaccgacag agtcgtgagt gaaaatgaaa atggtcgggt aatgtgaaag
     1381 agccgctaac cgcacagagg cacgcatgta atcggcaaag accaggaagg tcgcaccgta
     1441 cggaatcacg ccaccgaaaa ttgccatacc atttaggaca gaccccatcc catgttcgcg
     1501 cacaccgaag tggaagttac gtccagccgg agagtctttt tggaaagcag gcgaaccgtc
     1561 aattttggta ttgttcgaag gagcaaggtc tgccgagccg ccaataagct cagggagttt
     1621 tgccgccagt gcgttaataa ccttaccaga agcggcacga gttgccatac ctttcgggtc
     1681 agcggggaat gtcggtagtg cagattccca accttcgggc aatttgttca tcaatatgcg
     1741 acgattaaat tctgttccaa gatccgggaa gagccgtgca tacgcttcgc ggcgcatctt
     1801 ccaatcggct tcgagttcac gtccgcggtc aacggctttg cgataaaact caagcacatc
     1861 atcagggatc aagaaacgag gttccttggg ccagccaagg ttatcttttg cagcattcag
     1921 ttcttcatca cctagcggtt cgccatgtgc tttctgagtg ccttgtcggt tcggagcacc
     1981 aaaaccgatg atggtgcggc acaagatcaa cgacgggcgc gggtctgttt tagcagcttt
     2041 gatggcagaa tcaatcgctt ctacatcgtt accatcttct acacgttgaa tatgccaacc
     2101 gtaggcttca aagcgtttgg cgcggtcttc tgtgaaggaa aggtctgttg agccatcgat
     2161 ggaaatctta ttatcgtcgt acaggtaaat aagttttccg agcgagaggt gcccagccag
     2221 tgaagcggct tcagaagcga caccttccat caaatcacca tctgtaacga tggcatagat
     2281 gtacgagtca atcagttcgt gaccgggttt gttgaaagtg gcagcgagat gtgcaccagc
     2341 aattgccatc cccacaccgg tggagagacc cgcaccaagc ggaccagtgg ttacttccac
     2401 gccgggtgtc cagccgtatt cggggtgacc cggggttttg ctcccccact ggcggaaact
     2461 ctgcaagtct tccaaagata ggtcgtaccc ggtcaaatgg aggagagagt aaagaagcat
     2521 cgaaccatgc ccacccgaaa ggatgaagcg gtcacgtccc atccacttgg ggttacgggg
     2581 gctatgtctc agatggcgag tccagatggt gaaagccatc gcagcggctc ccatcgggag
     2641 accgggatga ccagaattcg ctttttgcac gccgtcagcg gaaagaaatc gaagtgtatt
     2701 gattgctttg ttttgaaatt cgaggtttgt catgggcggt attttaccat tcggttagta
     2761 tttttctttc aaatttttct cagggagggc gatctgccct tctgtagaat ctccaataag
     2821 ggaaaccttt tcaggtttat gaatcgacag ccaagccgta taggctgcca ccaatgcatc
     2881 caattgctca ggctgataaa gcatatctaa cggccaattg ccttttagca ttttatggcg
     2941 tgtgatctct tcaaaaaaat ccatcgggtc tttgatacga acgccggctt catacagaag
     3001 caattgacgt tgaattttcc cttccaatga aggctttggt tgcggtatcg cctcagccat
     3061 gacacaaaaa gcagcatgcg gctgagactc taaaatttga tagggcgtat tttcagttgg
     3121 atatttcttg aagcccatct tttccagttt acgataaagc gcaaaaccag attgtaccca
     3181 tgcagagcaa gcgctaattg tagaaggagt accagaaacc gtaatgccgc ggtctcgcaa
     3241 ttccaattct gccacgcgaa aaccagccgc acgcggcttc accgacttaa acatgtcttt
     3301 ctttttctcg cgcaccaacc cacgatttac gccagaaggc gcattgatcg caacaatcgc
     3361 tgaagattgc ccacccagga aggtcgtcac atcgtccagc tcgccatcag ccagagcgac
     3421 aagattcatc cccttatcca gtgcagcata cgtataggac ttatgggaag aagccgcgtc
     3481 aataccaaca aaaaccatgt ctgtaaaaag catagtaaga ttatctctta agagcacacc
     3541 gaatacaaaa caaacagtcc tattttactt ctaaaatctc atgaaccgaa cccaacgaat
     3601 cattctctac attcttttgc ttattgccgg ggcttcgtgg atcgtaatct ccggtcagcc
     3661 aaccggaaca accacagtcg ccgcagccgc ccctcaagcg gggtttaccg ccccagactt
     3721 taccctaaaa accgcagaag gtgaaacata caccctctcc gaactaaaag ggcaggcggt
     3781 tctcattaat gtatgggcaa cttggtgccc gccatgcaaa gccgaaatgc ccgccattga
     3841 aaaaatgtac aacgaataca agggtcaagg ctttaacgta ttagcgatca acagcacctt
     3901 tcaagatgat ccgcttcaga tcaaaccctt cactgaagaa tatagcttaa cctttcccat
     3961 cttattagat gaaacttcag atatcagccg tgattatcaa gtccgctcac tgccttcttc
     4021 gtattttgtc aatcgctatg gcatcatcac cgaagttgtg atcggtggcc ccatgtctga
     4081 agctttgctc cgcactaaaa ttgaagaggc tttgaaacca tgatcgaaac gttccaaaat
     4141 ttattcgcgc cgccacgtca catgatcttg ctcgttatcg ccgcatggct tggtcttgca
     4201 ttggcagaac ggcgtacaga acgtcacggc atcagcaaag aagacctcaa taacattacc
     4261 ttctatggat taattgcttt tgtcattggt gggcgcattt cgtatattat ccaaaacata
     4321 tcggcgttta caaaaagtcc acttggcatt gtttccatta acccaaatct cttcgaccct
     4381 atcggtgcaa ttgctgtagc atttatcgtt cttatagtat atggtcaacg caagcaactc
     4441 aacatttgga atatctttga ctcgctgaca ctttttttcg ctgtcctcgc cattgggctt
     4501 gggttaagcc accttgctgc gggcagtgca tttggactcc ctaccgatct tccgtgggga
     4561 attaacttat ggaatgccac acgtcaccca actcaaattt atgacacgct cgcctcttcc
     4621 atcatattga tcttgctctg gctcttcaaa ccaagtccac gcccaggcat caccttctta
     4681 acctatgctg catttgtagc cttagcacaa ctcatcttgc aagctttccg agctaattac
     4741 actattattt tttctgggct tcgtcaggaa caagtagttg catgggtggg cttactagta
     4801 tgtttcattc ttatagaagt taggctgaaa tcccaagaaa aacaataaat aaaagagctg
     4861 actaaagtca gctcttttat ttattgaaag cctatccaag ctcaggctca atcagaccat
     4921 agcttccatt gcgcctgcga tacagaacat taatcttgct cgtctctgca ttgtaaaaca
     4981 caaagaaatt atcgtgccct aaattacgca tttgcaccaa cgcttcttcc tcattcatag
     5041 gatacataac aaatttcttg cgttttgcaa acagcggtga aagttgcccg gtctcatcat
     5101 cgatgatgtc gtcgacagct tcagcagccg aacgtccatc gccacgtcca tgatacttct
     5161 tccctttatg cctttcaatc tgacgctgca tattatccaa tgctttgtca aatgcagcca
     5221 aggcttcgtc tgcgcgttct tctgagcgaa gggtaaatcc ctttccaaac acagtaatct
     5281 gcgcaacatt tcgatcattg acatcgcgtg cagcttggtg gtgcgaaagt tccacgcgcg
     5341 catcttcaat agctggcaag taatgatcga ggttgccagc cttcttcgaa acatactctt
     5401 cgatccgttc ggtcaagcga atatgacgtg tctgaacttc cactttgttg gacataagat
     5461 actcctttta ttaggttgtt aagttacaaa cacacgctat acatgtctca aatcatgatg
     5521 agacaatgca cgcgcgactg tcaacgcata tacttccttc gcacccgcag cataaagagc
     5581 ctctgcgcca gaggaaagag tcgaaccagt tgttgaaacg tcatccataa taagagctat
     5641 ttttccttgc acccctgatg cttgaaaagc gtcatgaaca ttcttctgtc tctcctccct
     5701 actcagcccc acttgagagc gcgtctcttt tcggcgcgcc aaagcgtgag gtgaatattg
     5761 caagcccaaa cccatagcaa gtggttttgc gatcattgca acctgattat atcccct
//
LOCUS       DAOQNJ010000095         5797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8034_length_5797_cov_9.057471, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000095 DAOQNJ010000000
VERSION     DAOQNJ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8034_length_5797_cov_9.057471"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PLG52_04240"
     CDS             <1..503
                     /locus_tag="PLG52_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40686.1"
                     /translation="AEESPIPNLKHVIAISPVLDPEKSTYAIDEFPLLRDYFRKKWTD
                     SMRKKQALFPELYQLYDVFSLPTIAEMTERMLQRHSDYPSASAYFRAYAVTGEALAAL
                     STPLTVITAQDDPIIPVSDFHHLKLSSTGELLIQPYGGHNGFLENIFGKAWYQRKIAE
                     ILLPLG"
     gene            562..2400
                     /locus_tag="PLG52_04245"
     CDS             562..2400
                     /locus_tag="PLG52_04245"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNB40687.1"
                     /translation="MKTSLMISYSRRQTPFVDRLYEELQKDGYSSIWLDYQSLTPAQP
                     WYQQILDGITGAETLLLVVSKDSIESKNVEPEWRLALEHKKRIVLVIFEAYPLPVELQ
                     GCEWVDFRLNHKASLQKLSNMLGMPPQPVTFPAPQSGFKAPKRFWLAIALSVIVLLSS
                     LPSWWTLIIPFILIPLPWQIYKRDYSFSRVIPTLTVLFPLFQIISVNWFFEEGSALSF
                     LRDIINLDWLPVVGVLAGWLLVGLLLTPVMQRRATPVAARVRFANPLVADIKKPQPVV
                     FAIDHAPEDGRYANALRRTLEKYGHRSIEPGETPQAVLVLISAFKKTTEYDLDTVAVY
                     PILLQAVTDIDRKLQRIQWIDFRKGMGSADKLAKLLPEPQKLLKVLAVAPTGSQEIFP
                     LAVNALQYFYLIAGVFGGGGLLISTLSFASLIASGVFGIEYISQIFVAILNGAFLFLV
                     AFSAVRGLRTRLGGSLALYPLLILMGFVYLINFANFSALFVDSNSEETSQLAFTAVNG
                     AGFALWFLPMGTFLIIPFLLFGWQGLYRWIPLRQTDNVTSLESIFLFYAPRKKGRLVF
                     HILFHLLLAVIVIAAMQLDSGEILTDLLVVVMMAVFFRWLARRNLK"
     gene            complement(2486..2989)
                     /locus_tag="PLG52_04250"
     CDS             complement(2486..2989)
                     /locus_tag="PLG52_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40688.1"
                     /translation="MGLFGFGKKANKNAPVLSADEVRTKILELNRETAPYQIIDGKDK
                     GVDLIAEWKIVDAKWYELFAKANITKVFRIYMKFDPAKNEVRAKDEEYTVEWRAGVPS
                     LSISVSKFQGQMTSVEFGAAYAFTEELKPGLVYNYRFNTNEIKKPIQEAVATCGWNYK
                     GVAFGKL"
     gene            3125..3661
                     /locus_tag="PLG52_04255"
     CDS             3125..3661
                     /locus_tag="PLG52_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40689.1"
                     /translation="MFFGKKETTEKKKNPIRLTILTDEYIIDALDDPDLSILQAVHDS
                     EIGDASGGVIILKDATLHSLRHASAPARTFSEWRLTSLVNMIAILSDDPVAQQLIAEG
                     WEDYQTPFKAFIYAGVYIIEGKMFSEDDTPPDFRIRTFAPLEDATITYALDQKEKTIR
                     GRWGLVNSVLMHGFSIEK"
     gene            3691..5091
                     /locus_tag="PLG52_04260"
     CDS             3691..5091
                     /locus_tag="PLG52_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40690.1"
                     /translation="MRKIFFVISIFVLTALACQSLTSTPLPSEPLTDSSTQAVSAPQA
                     TALPIGTSTPTSLPKGVIFVDTLQQEVYPFEENGKCSLAEAVIAANTGEPKDTCPAGV
                     PGESIISLMPGEYHFSQRDQSPPQFDWLLSLVKVGSALPPITFPLVIQGNGATLVRDE
                     GSEPFRFFEIMVNASLSLENMTLQNGDVQDDSGGAIYASNGSVTLNRVRFLNNHAEHG
                     GAVYLTFGALTVQESEFIENHAGFQGGGIHLDSAKSTIQNTKFISNTTDARGGALSAE
                     GVTLVVEDSLFLKNVTQDNRGGALHLEHVNVNITRSQFYQNQSAWIGAAIYINNPVMS
                     GTSEDEGDPLEQLDDTPMYVQMATLIPGYEATLQAHPSGVFQDFHEDMQVHQNCFANN
                     IILDTYEMNFSSAILARDVNAEDNYYGTASGPSGSGPGTGDGVGRSVKFAPFLTEPPA
                     YCDPELSQQVKENHNQ"
     gene            5101..5361
                     /locus_tag="PLG52_04265"
     CDS             5101..5361
                     /locus_tag="PLG52_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40691.1"
                     /translation="MAKLIPASERILRARKLIEQARALPVPTEGLGKSDFSYIANVRD
                     VLRQAKDMVKFIPQTAGVSLEMKEEVKKIYAEVEQAEKEILS"
     gene            5450..5707
                     /locus_tag="PLG52_04270"
     CDS             5450..5707
                     /locus_tag="PLG52_04270"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HNB40692.1"
                     /translation="MTDKLDALLEQVHAELQKVDEVDADSLKLLQELQQDVQGLLDKT
                     EMETPTVLARMQKAVQQFEVDHPALTALISELSTVLSNAGI"
BASE COUNT         1405 a         1458 c         1314 g         1620 t
ORIGIN      
        1 aagccgaaga gagtcccatc ccgaatctca aacatgtcat cgccatcagc ccggtgctcg
       61 accccgaaaa gtccacctac gcgattgatg aatttcctct gctgcgcgat tacttccgca
      121 aaaaatggac cgactccatg cgcaagaagc aggcactctt ccccgaactt taccagttat
      181 atgacgtctt ttccctcccg accattgccg agatgaccga gcgcatgctc caacgtcaca
      241 gcgactatcc ctccgcctct gcctacttcc gcgcctatgc cgtcactgga gaagcgctcg
      301 cggctctttc cacgccgctc accgttatca ccgcccaaga cgatcccatc atccccgtct
      361 ccgactttca ccatctcaaa ctatcgtcca cgggcgagct tctcatccaa ccctacggcg
      421 gtcacaacgg ctttctagaa aatatctttg gcaaagcctg gtatcaacgg aagatcgcgg
      481 aaatcttatt accgcttggt tgatacattc tctgcttata taatagatta aaaccaattc
      541 aatcaacgga ggaagccatt catgaaaacc agtttgatga tcagttattc acgccgccag
      601 actccttttg tagaccgact gtatgaagaa ctgcagaaag atgggtattc ttccatctgg
      661 ttggattatc aaagcttgac ccctgcccag ccctggtatc agcagatctt agatggcatt
      721 acaggggcag agaccctatt gttggtcgtt tccaaagatt cgattgaatc aaaaaatgtt
      781 gaaccagaat ggcggctggc acttgagcat aagaagcgca tcgttttggt aatctttgaa
      841 gcctatccct tgcctgtcga attacaaggt tgtgagtggg ttgatttccg cttgaatcat
      901 aaagccagtc tccaaaagct gtccaacatg ttgggtatgc ctccacaacc agttacattt
      961 cccgcacccc aaagcggatt caaagctccc aagcgcttct ggctggcaat tgccctgagt
     1021 gtaattgtcc tgctttcttc tctgccttcc tggtggacgc tcattattcc cttcatcctt
     1081 atcccgttgc cgtggcagat ctataaaaga gactatagtt tctcgcgtgt catccccacg
     1141 ttgaccgttc tatttccttt atttcagata atttcggtga attggttttt tgaagaaggt
     1201 agtgcccttt ctttcttacg tgacataata aatttggatt ggctgccagt cgttggtgtt
     1261 cttgcaggtt ggcttcttgt tggcttactg ctcaccccag tcatgcaacg acgtgccaca
     1321 cctgtggcgg cgcgtgtccg ttttgccaac ccactggtgg cagatatcaa gaagccgcag
     1381 ccggtcgtct ttgcaattga ccatgctcca gaagatgggc gttatgccaa cgctttacga
     1441 cgcacgctgg aaaaatatgg gcaccgttcc atcgaaccgg gagaaacccc tcaggcagtg
     1501 ttggttctca tttcggcgtt taagaaaaca acagaatacg acctcgatac tgtggcggtt
     1561 tacccgattc tgttacaagc agtcacagat attgatagaa aacttcagcg cattcaatgg
     1621 atcgacttcc gcaaagggat gggttcggca gataaattgg ctaaactttt gcctgagccg
     1681 caaaagttac taaaagtgct ggcagtcgcc ccaacaggct cacaggaaat cttcccattg
     1741 gcggtcaatg ctcttcaata tttttatttg attgcgggtg ttttcggggg aggagggctt
     1801 ttaatttcaa ccctgtcttt tgcatctttg atcgcatctg gagtttttgg cattgagtac
     1861 atctctcaaa tctttgttgc tatcttaaat ggtgcatttt tatttcttgt tgccttttct
     1921 gctgtacggg gtcttcgcac tcgtcttggc ggctcgcttg cactataccc tttattgatt
     1981 ttaatgggct ttgtgtatct tattaacttc gctaattttt ccgccttgtt tgttgattcc
     2041 aattcagagg agacttcaca gctggcattt acggctgtca atggggctgg gtttgccttg
     2101 tggtttttac cgatgggcac attcctaata attcccttcc ttctttttgg ctggcagggc
     2161 ttatatcgtt ggattccgct tcgccaaacc gataatgtca cctcgttgga aagtatattt
     2221 ttattctatg ccccccggaa aaaagggagg cttgttttcc acattttgtt tcacttgctc
     2281 ttagcagtta ttgtgattgc agctatgcaa ttggattcag gtgaaatact cactgatttg
     2341 ctagtagtag ttatgatggc agtatttttt cgctggctgg cgaggcgtaa tttaaaataa
     2401 acattgcctt gaatgaacaa taaataacca cgccgttctt cttcaagagc ggcgtggtta
     2461 tttatgcagg ctttttaaac gagttctaca gcttaccgaa cgccacacct ttgtagttcc
     2521 aaccacaagt cgctacggct tcctgaatcg gcttcttgat ttcattggta ttaaagcgat
     2581 agttgtagac caatcccggc ttcaattctt cggtaaacgc ataggctgca ccaaattcca
     2641 ccgaagtcat ctgtccctga aacttcgaga ccgagatcga aagcgacggc acgcctgccc
     2701 gccattccac ggtgtattct tcatctttag cacgtacttc gttctttgcc gggtcgaact
     2761 tcatgtaaat gcgaaagacc ttggtgatgt ttgcctttgc aaagagctca taccatttcg
     2821 catctacgat cttccactcc gcgatcaggt ccacaccttt gtctttcccg tcaatgattt
     2881 gatacggcgc cgtttcacgg ttcaattcca atatcttcgt ccgcacttcg tctgctgaga
     2941 gaaccggtgc gttcttgttt gctttcttgc cgaaaccaaa gagtcccata acaatgtcct
     3001 ttcgaattga tgaactgact ttattatacg agttgcttcc acattctcta tcccccagat
     3061 aggcgattgg ggaaatcccc gctccttcat ataattgtct gcgtctatat ggaaggagag
     3121 taccatgttt tttggtaaaa aagaaaccac tgaaaagaaa aagaatccca ttcgtctaac
     3181 catcctgaca gatgaataca ttatcgatgc cttggatgac ccagacctgt ccattttgca
     3241 ggcagtccac gactcagaaa tcggggatgc atccggcggc gtaattatct tgaaagacgc
     3301 gaccctacat tcgcttcggc acgcgtcggc tccggctcgc accttttccg aatggcggtt
     3361 gacctctttg gtaaacatga tcgccattct ttcggatgat cccgtcgcac agcagttgat
     3421 cgcagaaggc tgggaagatt atcagacgcc tttcaaggct tttatctacg caggggtcta
     3481 catcattgaa ggcaagatgt tttcagaaga cgatactccg cccgatttta ggattcgcac
     3541 ctttgccccg ctcgaagacg ccacaatcac ctacgcatta gaccaaaaag aaaagaccat
     3601 tcgcgggcgc tggggattgg tcaactcggt tctcatgcat ggctttagta ttgaaaaata
     3661 aattttatcg tttctgtttt ggagtatgtt gtgagaaaga ttttttttgt aatttcgatt
     3721 ttcgttttaa cagcgttagc ttgccagtcc ctgacgagta ccccgcttcc ttctgagccg
     3781 ctgactgatt catccaccca ggctgtttct gccccgcaag cgacggctct gcccatcggc
     3841 acgtccacgc caacttctct gcccaaaggg gttatcttcg ttgatacatt gcagcaagaa
     3901 gtgtatccct tcgaagagaa tggtaaatgt tcactggcag aggctgtcat tgctgccaac
     3961 acaggcgaac ccaaagacac ttgtcctgct ggtgtgccgg gtgaatccat tatcagcctc
     4021 atgcccggag aatatcattt cagtcaacgt gaccaaagtc cgccgcaatt tgattggttg
     4081 ctaagcttgg tcaaggttgg cagcgccttg ccgccgatta ctttcccatt ggttattcaa
     4141 ggtaacggcg cgacccttgt ccgcgatgaa ggctcggaac ccttccgctt ctttgaaatc
     4201 atggtcaatg ccagcctcag ccttgagaat atgactttgc aaaatggcga cgtacaagat
     4261 gactcgggcg gtgcaatcta tgcctcaaat gggtctgtca ccttgaaccg cgtccgtttt
     4321 ttgaacaatc atgctgagca tggcggggct gtctatttaa cctttggggc actgaccgtt
     4381 caagagagcg agtttattga gaatcatgct ggctttcaag gtggcggtat tcacctcgac
     4441 tctgccaagt ccacgattca gaatacgaaa ttcatcagca acaccaccga cgcgcgcggt
     4501 ggtgcgcttt ctgccgaggg ggttaccttg gtggttgaag acagtttgtt cttgaaaaat
     4561 gtaacccaag ataatcgtgg cggcgctttg catttggaac atgtcaacgt caatataacg
     4621 cgcagtcagt tttaccagaa ccagtctgca tggatcggag ccgcgatcta catcaacaac
     4681 ccggtgatga gcggtaccag cgaagatgaa ggggatccgc tcgaacagtt ggatgatacg
     4741 cctatgtatg ttcaaatggc gactcttatt cctggctatg aagcgacctt gcaggctcac
     4801 cccagtgggg tgtttcaaga ctttcatgaa gatatgcagg tccatcagaa ttgttttgcc
     4861 aataatatta ttttggatac ctatgagatg aacttctcaa gcgccatcct tgcgcgtgac
     4921 gtcaatgccg aggataatta ctacggcacc gctagcggac cctccggcag tggtccaggc
     4981 accggagatg gggtaggtcg gtctgtgaaa tttgcaccct tccttaccga accgcctgcc
     5041 tattgcgacc ctgagttgtc acaacaagtg aaagaaaatc ataatcaata ggatttcatc
     5101 atggcaaaac ttatccctgc atctgaacgc atcttgcgcg cccgaaaact gatcgaacaa
     5161 gcccgggctt tacccgtccc gacagaaggt cttgggaaaa gcgatttttc ctatatcgcc
     5221 aatgtccgcg atgttttgcg tcaggcaaag gatatggtca agttcattcc acagacggcg
     5281 ggtgtttcgc tcgaaatgaa ggaagaggtc aagaagatct acgctgaagt ggaacaggca
     5341 gagaaggaaa tattgtcata atcaaggttt tatgacgtta ttcactggaa gcagccttgg
     5401 gttggttgta caatgaaagt gaacctgatc ctcgaaagga gccaacccca tgaccgataa
     5461 actcgacgca cttctggaac aagtccatgc cgagctccaa aaagtggacg aagtggatgc
     5521 cgacagcctc aaacttttac aagaactgca acaggatgtg caaggattgc ttgacaagac
     5581 cgagatggag acgccaaccg tgcttgcccg aatgcaaaag gcggtgcagc aatttgaagt
     5641 agatcaccct gctcttacgg ctttgatctc tgaactatcc acagtattaa gcaacgccgg
     5701 aatttgaaag agcccgagac cgctatgaag gaattttcct ttatgacggt cttttttatt
     5761 tttgatgatc ggaattcttc atctgacctc taccttt
//
LOCUS       DAOQNJ010000096         5795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8043_length_5795_cov_9.593728, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000096 DAOQNJ010000000
VERSION     DAOQNJ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8043_length_5795_cov_9.593728"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..522)
                     /locus_tag="PLG52_04275"
     CDS             complement(7..522)
                     /locus_tag="PLG52_04275"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNB40693.1"
                     /translation="MKKIFAFLFASALLLSACGGGMAGGEEHTDGEGVEAHDYWVRAA
                     MAGGNSAAYMLLHNHGAEDDALIAVSSDLADAVEMHLSQMKADGTMEMIQQEKIDLPA
                     DGEVELKPGSYHIMFIGLKQDLVAGNEITLTLTFEKAGDITLTVPVKDSADMGGESMN
                     MGGMDMATPTP"
     gene            complement(556..1128)
                     /locus_tag="PLG52_04280"
     CDS             complement(556..1128)
                     /locus_tag="PLG52_04280"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HNB40694.1"
                     /translation="MATNSLDREQKILEHLKTHKTASIQELAEAFGVSNMTIHRDLHK
                     LANAGQIIKKHGGAALPNSGVKALEEPSSCAMCGKPASQRTIFIVKFDNGEELRACCA
                     HCGLMMQSREKNVWQSLTVDFLYGHMISAGQAYYLVGSELNICCVPSILAFGSKADAE
                     KFQKGFGGQLLDMENTIHHMHGMMHMHSDM"
     gene            complement(1208..2371)
                     /locus_tag="PLG52_04285"
     CDS             complement(1208..2371)
                     /locus_tag="PLG52_04285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB40695.1"
                     /translation="MPYLILSTILFLLALIIIHWIHNQYHLDIAVTPVPAPESAPKVS
                     VCIPARNEETNIRRCVEAVLRQDYPNFEIIVLDDRSTDSTLTLLKEIASRDSRLLPIS
                     GSDLPEGWAGKPHALYQASAVAKGEWLCFVDADTFLAPNALSAVYAKASETKADLFTV
                     MTKQILGSFWERTVMPLVMTALSVGFSPRKVNDPSRRDAVANGQFIFIKRSIYDLIGG
                     HEKIKDQIVEDKALSENVKWNGHRLVVANGMQLISTRMYTSLETMWEGWTKNIYLGLR
                     DHPSLLLLGAFGATLALLAALFLPVWPLLGLGWFLNEGGWMALAVIVEALFVWGYLIV
                     IRARIAQEMDIPRWYAWTTPLGAGVFAAMMLTSAWKVISGQGVTWRGRNYKPK"
     gene            complement(2436..5018)
                     /locus_tag="PLG52_04290"
     CDS             complement(2436..5018)
                     /locus_tag="PLG52_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNB40696.1"
                     /translation="MIPLHLRIAGFLSYRDPVDLDFNMVDLACISGHNGAGKSSLLDA
                     FTWSLFGEARGKGVDVINLSQDVKAAEVALTFLYEGNIYRVQRTLPRNKSTVLEFQVL
                     DRGSQTTDDGRWRPLTEKTTRDTQARIEQTLRLDYETFINASFFLQGKADQFTQKKAS
                     ERKAVLSAILGLEIWDTYKDRTAERRRELETEVTEIDGRIAEIDAELGEEDERRRRLS
                     ELEESLKQLTTAREAQESALENIRRNAALINEQRKTVANAETQLKRVQDTLTSIEAKI
                     AVKENERAEYLDLLERAKDIESAYKAWQKARKELEAFDANATEFREHDEKRQPLLREI
                     EVEKAKLEQEGEELGKQYSVINDYVAMVESLESQLNTARESLKEAEEKIQERTKLEAQ
                     RNEARERQAGLKAENESLRREMDELKTRIDALKVAEGAECPLCGQPLSESHRKSTLKD
                     LEQTGKDKGDAFRSNKKEAEDLVEQIANYESRITKLSGADNERVRFSNSISQLTLQVE
                     SIRQQSKEWEKTGAKRLKEIEKVLESGKYASEAQKALKVLDKELTKLGYDVSAHEAKR
                     KEENDLRYVEEESRKLESARELSKRIGKEIQELEEERGKKKEETAEAEKVFSEAENVL
                     AESEAGAPNLQDAESKLFELREDENRVRDEAGAARQRVDVLATLRNRRAEYERQREEI
                     SQNIVRHKSLERAFGKDGVPALLIEQALPQIQQKANDILERLSDGTMMIQFVTQAEYR
                     DKKRDDLKETLDIHISDSSGMRDYEMYSGGEAFRVNFAIRLALSEILAQRKGARLQTL
                     VIDEGFGSQDALGRQRLVEAINAVKNDFSKIFIITHLDELKDAFPNRIEIEKTERGSS
                     VRVV"
     gene            complement(5132..5485)
                     /locus_tag="PLG52_04295"
     CDS             complement(5132..5485)
                     /locus_tag="PLG52_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40697.1"
                     /translation="MSVVVGVGVLVIVAVMVGVFDAVGVNVLVGVKVRVVVGVNVNVL
                     VLGRKNVSVAVGVDVDVGVEVIVGVNVIVPVTIKGVLLAVAVFGVPVTVSVMDGVGVT
                     SRGLGAKERQTNPAQ"
     gene            5509..>5795
                     /locus_tag="PLG52_04300"
     CDS             5509..>5795
                     /locus_tag="PLG52_04300"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40698.1"
                     /translation="MSDILPFLKSIISISGVSGDEAPVADLVQKKWTPLVDEISQSRL
                     GSIHGLKQGTLKKSGSKATASSRPSVMIATHMDAIGMMVTRIVDGFIYFTNV"
BASE COUNT         1399 a         1612 c         1212 g         1572 t
ORIGIN      
        1 aaaaaattac ggggtcgggg ttgccatatc cattccgccc atgttcatgc tttctccacc
       61 catatctgca gagtccttca cagggacggt caaggtgata tcacccgctt tttcaaaggt
      121 cagggtcagt gtaatttcgt tgccagccac aaggtcttgc ttcagaccaa tgaacatgat
      181 gtgatagcta cccggcttca attccacttc accatcggca ggcaaatcaa tcttttcttg
      241 ctggatcatt tccatggtgc cgtcggcttt catttggctc aggtgcattt caaccgcatc
      301 tgccaaatca gaactgacag caatgagggc atcatcttct gccccatggt tatgaaggag
      361 catataagca gcgctattgc cacctgccat cgctgcgcgg acccaataat catgagcttc
      421 cacaccttcc ccatcggtgt gttcttcccc acctgccata ccacccccgc atgcacttaa
      481 cagaagcgca ctggcaaaaa gaaaagcaaa aatttttttc attctcttat tcctcacaaa
      541 ttttattttt ctgaatcaca tgtctgaatg catgtgcatc attccatgca tatggtgaat
      601 agtattttcc atatcaagca attgcccacc aaagcctttt tgaaacttct ctgcatctgc
      661 ctttgagccg aatgcaagaa tacttggcac gcagcaaata tttagctcgc tcccaacaag
      721 gtaataagcc tgcccagcac tgatcatatg accatataaa aaatctactg tcagagattg
      781 ccaaacgttc ttttcgcggc tttgcatcat tagaccgcaa tgagcacagc aagcacgcag
      841 ttcttccccg ttatcgaatt tgacgatgaa gatcgtcctt tgagaagcgg gtttcccgca
      901 catggcacat gaagatggtt cttctaatgc ttttacgccc gagttcggta aggcagcacc
      961 gccatgcttc tttattatct gacccgcatt tgctaactta tgcaaatcgc ggtggatggt
     1021 catattcgac acgccaaaag cctcagccag ttcttggatt gaggcagttt tatgtgtctt
     1081 gagatgttca aggatctttt gttcgcggtc taaggagttg gtagccatat gttatatttt
     1141 gttatcttta acaagagatt acgaaatata acaactcaac caatatttat caagatgaca
     1201 tttatcacta ctttggctta tagttccgtc cacgccaggt aaccccctgc cccgaaatca
     1261 ctttccatgc cgaggtcaac atcatggcgg caaacacccc tgccccaagc ggagtggtcc
     1321 acgcatacca gcgcggaatg tccatctctt gcgcaattct ggcgcggatg acaatcaaat
     1381 agccccacac aaataatgcc tcaacaatca cagccaatgc catccaccca ccttcattga
     1441 gaaaccagcc taaacccaac aacggccaca ctggaaggaa caacgcggca agcaatgcca
     1501 aggtagcacc aaatgcgccg agcaataaca acgatggatg gtctcgcaag ccaaggtaga
     1561 tattcttcgt ccagccttcc cacatggttt ccaaagacgt gtacatgcga gtgctaatca
     1621 gttgcatgcc atttgccacc accaagcggt gaccattcca ctttacattt tcagagagcg
     1681 ctttatcttc cacaatttga tctttaattt tttcatgccc gccgatcaaa tcatagatac
     1741 tgcgcttgat gaagatgaac tgtccgttcg ctaccgcatc acggcgcgat gggtcattga
     1801 ccttgcgcgg agaaaagcct actgagagcg cggtcatcac caacggcatc accgtccgct
     1861 cccagaagga accgaggatt tgcttcgtca tcacggtgaa caaatcggct tttgtttcgc
     1921 tggctttggc atacacggcg gaaagcgcat tcggcgcgag gaaggtatcg gcatctacaa
     1981 agcaaagcca ctcgcctttc gccacagcag aggcttggta caaagcatgg ggtttgcccg
     2041 cccagccttc gggtaagtct gagccgctga ttgggaggag acgagagtcg cgggatgcga
     2101 tctctttcaa tagagtcaga gtggaatcgg tggagcgatc atccaaaacg atgatttcaa
     2161 aatttgggta atcttgacga agaaccgcct caacacaacg ccgaatgttg gtctcttcgt
     2221 tgcgagccgg gatacacacc gaaaccttcg gcgcggactc tggcgcaggc accggcgtga
     2281 cggcgatatc aagatgatat tggttatgaa tccaatggat gatgatgaga gcgaggagga
     2341 aaaggatggt ggagagaatc aagtagggca tgggtaaata gtaattggta attagagctt
     2401 gatgcttaat atttaccaat tactaatctc ttgaattaca ccacccgcac ggaacttccc
     2461 cgttccgttt tttcgatctc gatgcgattt gggaaagcat ctttgagttc gtcgagatga
     2521 gtaatgatga aaattttaga gaagtcattt ttgacagcgt taatcgcttc aacaaggcgt
     2581 tgacgtccca atgcatcttg cgagccaaaa ccttcatcaa tgacgagcgt ttgcaaacgt
     2641 gcacctttgc gttgggcaag gatttcagag agtgccagcc gaatcgcaaa gttgacgcgg
     2701 aaggcttctc cgcccgaata catttcataa tcacgcatgc ccgaagaatc gctgatgtga
     2761 atatcaagcg tttctttcaa gtcgtcgcgt tttttatcgc gatactctgc ctgtgtgacg
     2821 aattggatca tcatcgtacc atcgctgaga cgttcgagaa tatcgttggc tttctgctga
     2881 atttgcggaa gcgcctgttc gatcaacaac gctgggacgc catctttgcc aaaggcacgc
     2941 tccagcgatt tatgacgcac aatattctgg ctgatttcct cgcgttgacg ttcatactct
     3001 gcccgacgat tgcgaagggt agccagcaca tccacccgct gacgggctgc accggcttca
     3061 tcgcgcacgc ggttttcgtc ttcgcgcaat tcaaaaagct tgctctcggc atcttgcagg
     3121 ttcggagcac ccgcttcaga ttcggctaaa acgttttcgg cttcgctaaa caccttctca
     3181 gcctcagcag tttcttcttt cttcttgcct cgttcttctt ccagttcttg aatctctttg
     3241 ccaatacgct tactcaattc tcgggcagac tctaacttgc gagattcttc ctctacatag
     3301 cgcaggtcgt tctcttcttt gcgttttgct tcgtgagcag aaacgtcata cccaagcttg
     3361 gtaagctctt tatcaagcac tttgagcgct ttctgtgctt cactcgcgta ctttccgctt
     3421 tctaatacct tttcaatttc tttcaagcgc ttcgcgccgg tcttttccca ttctttgctc
     3481 tgctgtcgaa tcgactctac ctgcaaagtc agctgtgaaa tggaattaga gaagcgcaca
     3541 cgttcgttat cagcgcctga taatttcgta attcgcgatt cgtaatttgc gatctgttca
     3601 acgaggtctt ctgcttcttt tttgttagaa cggaacgcat cgcctttgtc tttgccggtc
     3661 tgctccaaat ctttcagagt agatttacga tggctttcgc tcaaaggctg cccacacagc
     3721 ggacattccg caccttccgc aactttcaag gcatcgatgc gggtcttgag ttcatccatc
     3781 tcccgccgca gagattcatt ctccgctttc aagcctgcct gtcgctcccg cgcttcgttg
     3841 cgctgagctt ccaatttagt gcgttcttga attttttctt ctgcttcttt tagcgactct
     3901 cgcgcagtat tcaattgact ttccaggctt tcaaccattg ccacatagtc attgattact
     3961 gaatactgtt taccgagttc ctccccttct tgctccaact tcgccttctc tacttcaatt
     4021 tcgcgcaaca agggttgacg tttctcatca tgttcgcgga actctgttgc gttggcatcg
     4081 aacgcttcca actctttacg cgctttttgc cacgccttat acgcggactc aatatccttt
     4141 gctcgttcta acaaatccaa atactcggcg cgttcatttt ccttgacagc gatcttcgcc
     4201 tcaatggaag taagcgtatc ttgtacccgt ttcaactgcg tttcggcgtt cgcaactgtt
     4261 tttctctgct cattgatcaa agctgcattt cggcggatgt tttccaatgc agattcttgt
     4321 gcttcgcgcg ccgtcgttaa ctgcttcagt gattcttcta attcgcttaa acggcgtcgg
     4381 cgttcgtctt cttcccctaa ttccgcgtcg atctccgcaa tgcgaccatc aatttcagtg
     4441 acttcggttt ccaattcacg acgacgttct gccgttcggt ctttgtaggt atcccaaatt
     4501 tccaacccga gaatggcgct tagcactgcc tttcgctcgc tggctttctt ctgcgtgaat
     4561 tggtcggctt tgccttgcaa aaagaacgaa gcattgataa acgtctcata atcgaggcgt
     4621 aaggtttgtt caatgcgggc ttgcgtgtca cgggtcgttt tctccgtcag cggacgccac
     4681 cgtccatcgt cggtcgtctg tgacccacgg tctaagacct gaaactccaa aaccgtactc
     4741 ttattgcgtg gcaaagtccg ctgcacgcga taaatattgc cttcatataa aaacgtgagg
     4801 gcaacttcag cagcttttac gtcctggctt aggttgataa catccacccc tttaccgcgt
     4861 gcctccccaa acaatgacca tgtaaacgca tccaataaag aagacttgcc cgcgccgtta
     4921 tgcccagaga tacaagcaag gtctaccatg ttgaaatcca agtcaacagg gtcacgataa
     4981 gagaggaaac cagcaatacg aaggtgaagt ggaatcatta aatgctaccc aatcgcatgg
     5041 attataatcg aaccactatg ctagacgccg aaacacctca agcacgccgc cgcatgcggc
     5101 ttctcgcaat cattattgca tccataccct gttattgtgc cgggttcgtt tgtctttcct
     5161 ttgctcccaa tccgcgcgat gtgaccccaa ccccatccat cacagaaact gtgacgggca
     5221 ccccgaacac agccaccgcc agtagcacac ctttgatcgt aaccggcaca attacattca
     5281 cgccaacaat cacttccacg cctacatcaa catccacacc tacggctacg ctcacgttct
     5341 tcctgcctag caccaacaca tttacattta ctcccaccac aaccctcacc ttcacgccta
     5401 ccaatacatt tacaccaacg gcatcgaaca cgccaaccat tacggcaacg atcactaata
     5461 cgccaacccc aacaaccaca ctcactccat cccctacccc ataacttcat gagcgatatt
     5521 ctccccttcc taaaatcgat tatctccatc tctggtgttt ctggggatga agcccccgta
     5581 gcggacctcg ttcaaaaaaa gtggactccg ctggttgatg agatctccca aagtcgcctc
     5641 ggctcgattc atggactgaa gcagggtacg ctaaagaaaa gcggatccaa agccacagcc
     5701 tcttcccgcc catcggtgat gatcgccacc cacatggatg ccatcggtat gatggtcacc
     5761 cgcattgtgg atggctttat ttatttcacc aacgt
//
LOCUS       DAOQNJ010000097         5774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8086_length_5774_cov_6.985662, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000097 DAOQNJ010000000
VERSION     DAOQNJ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8086_length_5774_cov_6.985662"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="PLG52_04305"
     CDS             <1..1408
                     /locus_tag="PLG52_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNB40699.1"
                     /translation="PAIDLLITEDSARARVLATQIEGLNAQRRMLTKQVNDAAEAQLK
                     ENPELLNHPVIILSHPNWPGGVVGIVANKLVERYHKPAILFNESDDGILRGSARSIEG
                     LHITKAITTQKDLLMGFGGHPMAAGMALHKENLPAFRRGLGKAVEKQLGDIVFEEPTL
                     QIDAWLGLTDLSLDLADSLELLAPFGAGNPQLTLATRNVTLKSAISLGKTKEHLRLNV
                     EDENGEVASFLWWGGAGEELPPKDSKFDIAYTLRATSFRGQRQVTLQFKEFRVTEERP
                     IEVKEAKLDIRDMRLQHTLVNAQPSALIWAEGPDRAIGVNRLELTQAKELTIYTTPPS
                     SIELRKALDIVQPEVVYFFGISPAEQSPEEFLTYLAGLCKFVLNQRHGKTTISELAAA
                     SSARESAIQLGLEWLAAGGQLTVTLEENEVQLSKETQEKNPYLQMELFIALRGVLSET
                     SAYRKYFATVPDLKTVFR"
     gene            1419..1862
                     /locus_tag="PLG52_04310"
     CDS             1419..1862
                     /locus_tag="PLG52_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HNB40700.1"
                     /translation="MTNRVLFICTGNYYRSRYAEIYFNTIAQNLSIDWEADSCGLDTS
                     GGHNVGPISKFALERLKIRNISPDPLRYPRQLEEKDLQEASLIIAINRVEHQPMIERQ
                     FPAWADRITYWEIADLNITSADDALSALESNVEKLVANLATSHNQ"
     gene            complement(1843..2349)
                     /locus_tag="PLG52_04315"
     CDS             complement(1843..2349)
                     /locus_tag="PLG52_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40701.1"
                     /translation="MTDSPANLLCKACGLCCSGHLFSWVRLDANELDPIAKLGIDVIR
                     DDPRQRGFLQPCPMWNGVCTIYTIPEYPRSCKRYKCKVFRQLVDDDISFPAAMSIIQE
                     TLTLIREIEPLLPVSEIPSFRERLIAHKEELEAKKEVDNDFMQKTLTLLTRYEDLFGV
                     DDFIDYDS"
     gene            complement(2444..3598)
                     /locus_tag="PLG52_04320"
     CDS             complement(2444..3598)
                     /locus_tag="PLG52_04320"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNB40702.1"
                     /translation="MTDLNQAIQLIRQGQKFEAQRILQEIIKTEPKNIPAWFWYVETC
                     ATTERRIQVLETCLKMNPGNLQVEQALQIFRNSQPPVPAPPKVEPPTYQSNTPEPQKN
                     DDLYGYTSFRSDSFEQTQSYEEDKSPAYDSPLYEYETFRTNSSVKEESQKKQPWEMDL
                     STYEDNSMLAKSKKVRRSYSAFDVWLTALTSQDEKSYADLLEDPEAGLERAFSWVAIA
                     GLITALTVPVQYFLNPNLGELLDIPELQRQVGNMTPNAFIIILTMISFVTAPIGAILN
                     LALSGGIQNLLARFFGGGGNFKRTAYALSAFLAPMAVVTSLVAIVPVAGGCLSFPLGI
                     YSFILNVRALKASHSLTNGAAIGVLLAPSILVIIFGCLFLFMSGTSLSSS"
     gene            complement(3670..5502)
                     /locus_tag="PLG52_04325"
     CDS             complement(3670..5502)
                     /locus_tag="PLG52_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNB40703.1"
                     /translation="MYGWHLRILRVNLTTRKVTTEDVDPQIARDYLGGRGWAIHYMYK
                     EMDPAVDPLSPENMLIFATGPLTATPAPTGNRYMVVTKSPLTGALAHSNSGGEFPTWM
                     KRTGFDMFIFEGKASEPVYVLVNEDQIEIKSAAHVWGKDTHETTDILKAETSDEARVA
                     CIGPAGENLALMAAIMNDKHRAAARSGVGAVMGSKNLKAVVAMGNKNPPLHDSEGMRA
                     ISVETSKNVGADVKKGSNMRIYGTSYVPQVTNTLGILPTRNFLQGTFEDVHNVDGDAL
                     KNQYLIRHTPCYRCPLSCGRLTEVPDGPYKGKGEGPEYETISSLGTGCGVSNLAALVK
                     ANYLCNEYGMDTITTGMTIAVAMEMYEKGYLTEETIGMPLKFGDHDAMIEMIKLMAYN
                     KGFGQDLAQGSYRLAEKYGHPELAVTTRKQEFPGYDPRGSQGMGLLYATSNKGASHME
                     GDVAYEEVFGVPIKENPLSTEGKPELVKHFQDSFALIDSSGLCVFVAIRYVFDKNRLI
                     WPQTLAAMMNLSTGAGYTPEEVMKAADRVYTLERMFLIKAGSGPEWDVLPYRMLHEPL
                     PDGPAKGKVAELDKMLPEFYEKRGWDEKGYPTQAKLMELGLPLN"
     gene            complement(5558..>5774)
                     /locus_tag="PLG52_04330"
     CDS             complement(5558..>5774)
                     /locus_tag="PLG52_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNB40704.1"
                     /translation="ANYRDALPPGHKHGVIELDVPEGTTVYDAISRFDIPLNDESVIV
                     LNGLTVDMDTPLREGDMVTAFSAIAGG"
BASE COUNT         1563 a         1419 c         1338 g         1454 t
ORIGIN      
        1 ccccgccatc gacctgttga ttaccgaaga ctctgctcgt gcacgagtac tcgcgactca
       61 aattgaaggc ttaaatgccc aacgtcgcat gctcaccaaa caagtcaacg atgcggcgga
      121 ggcacaactc aaagaaaacc ccgaactgct taatcatcca gtcatcattc tttcgcatcc
      181 taactggccc ggcggcgtgg tgggcatcgt cgccaacaaa ctcgttgaac gctatcacaa
      241 acccgccatc ctcttcaacg aatcagacga tggcattctg cgcggttcgg cacgttccat
      301 cgagggtttg cacatcacca aagccatcac cacccaaaaa gatctgctga tgggtttcgg
      361 cggacatccc atggcggcgg gcatggcatt gcacaaggag aatcttcctg cctttcgccg
      421 cggacttgga aaagccgttg aaaaacaact tggggatatt gtctttgaag aacccacgct
      481 tcagatcgac gcctggctgg gtctcaccga cttaagcctt gacctcgccg atagcttgga
      541 gctactcgct cctttcggcg ccggcaaccc acagctgacc ctcgccacgc gcaatgtgac
      601 tctcaagtct gccatttcgc ttgggaaaac caaagaacat ctacgcctaa acgtagaaga
      661 tgaaaacggt gaggttgcca gtttcttatg gtggggcggc gccggagaag aacttccacc
      721 caaagacagt aaattcgata ttgcctacac tttacgtgcc acatccttcc gtgggcaacg
      781 tcaagttacg ctgcaattta aagaatttcg agttactgaa gaacgcccta tcgaagtcaa
      841 agaagcaaag ctcgatattc gagatatgcg tttgcaacat actttggtta acgcacaacc
      901 atccgccctc atttgggcag aaggacccga tcgcgcaatc ggagtcaacc gtcttgaatt
      961 gactcaagct aaagaattga ccatctatac gacgccgcct tcttctattg aactacgaaa
     1021 agccttggat attgtccaac cagaggttgt ttacttcttt ggtatttccc ctgccgagca
     1081 aagccctgaa gaattcctca cgtatctagc agggttatgc aaattcgtcc ttaatcaacg
     1141 ccatgggaaa accaccattt ctgaactcgc cgccgcatct tctgcacgag agtctgccat
     1201 tcaactcggt ttggaatggc tggcagccgg cggtcaattg accgtgactc ttgaagaaaa
     1261 tgaagtccag ctttcaaaag agacacaaga aaaaaatccg tatctacaga tggaactttt
     1321 cattgcgcta cgtggcgtct taagtgaaac ctctgcatac cgaaaatatt tcgcgacagt
     1381 tccagaccta aaaactgttt ttagataaaa aaagcaaaat gacaaatagg gttttattta
     1441 tttgtaccgg caactactat cgcagccgat atgcagaaat ttatttcaat acaatagctc
     1501 aaaacttaag tattgattgg gaagctgact catgcgggct ggatacatca ggtggacaca
     1561 atgtgggtcc gatctcgaaa tttgcgttgg aacgcctcaa aatccgcaat atctcacctg
     1621 accctttgcg gtatccgcga caattggaag aaaaagactt gcaagaagca agccttatca
     1681 ttgcgatcaa tcgagtggaa catcagccaa tgattgaaag acagttccca gcatgggctg
     1741 accgtatcac gtattgggaa atagcagact taaatatcac cagtgcggac gatgcactat
     1801 ctgcccttga gagcaacgtt gaaaaactcg tagcaaacct tgctacgagt cataatcaat
     1861 aaaatcatct acaccaaaca gatcttcgta gcgggttaac aaggtcaaag ttttttgcat
     1921 aaaatcgtta tctacttctt tcttcgcttc caattcttct ttatgtgcga tgaggcgctc
     1981 tcgaaaactg gggatttctg acacaggtaa aagcggctca atttcgcgga tcaaggtcag
     2041 cgtctcttga atgatagaca tagcagccgg gaaagaaata tcatcgtcca caagttgacg
     2101 gaacactttg catttataac gcttgcaact acgggggtat tcagggatag tgtagattgt
     2161 gcagacccca ttccacatgg ggcaaggttg caaaaatccg cgttgacgtg gatcatcacg
     2221 gatcacgtca atgcccagtt tggctatcgg atcgagttca tttgcatcga gccgaaccca
     2281 cgagaaaaga tgccctgaac agcatagtcc acaggcttta cagagaaggt ttgctggaga
     2341 gtcggtcatg tatggatgat aacaaaggta cgagaaactg aatcaactat cccttaagaa
     2401 agaagcgggc gcatttggaa gcatccgctt ctttttggat attttaagat gacgataatg
     2461 aagtccctga cataaaaaga aataaacacc caaatataat aaccagaata gatggtgcta
     2521 ataagacgcc gattgcagcg ccgtttgtca aagagtgaga agccttcaag gcgcggacgt
     2581 tcagaataaa actataaatg ccgagcggaa aagaaaggca accgcccgca accggcacaa
     2641 ttgccacaag ggaagtaacc actgccattg gtgcaaggaa tgcagataat gcatacgctg
     2701 ttcgtttaaa gttgccgcca ccaccgaaga accgcgccaa caaattttgg atgccccccg
     2761 aaagtgcaag attaagaatc gctccaatag gcgccgtgac aaaagagatc atggtcagaa
     2821 taataatgaa ggcattgggc gtcatattcc caacttgccg ctgcaattcg ggaatatcaa
     2881 gcaattctcc taaattaggg ttgagaaagt attggacggg aacggtcaaa gccgttatta
     2941 acccggcaat ggcgacccaa ctaaaagcac gttcaagacc tgcttctggg tcttccaata
     3001 ggtcggcata tgatttttca tcctgagagg ttaaggctgt caaccataca tcaaacgcag
     3061 aataactacg ccgaaccttt ttagacttgg ctaacatgga gttatcttcg taggtagaca
     3121 aatccatttc ccaaggttgc ttcttctgag actcttcttt cactgatgaa tttgtgcgaa
     3181 atgtttcgta ctcatacaag ggcgaatcat atgctggaga tttatcttct tcataagatt
     3241 gtgtctgctc aaaagaatcg gaacggaatg aggtgtaacc gtacaaatcg tcgttctttt
     3301 gaggttcagg agtgttgctt tgataagtgg gcggttcgac cttgggagga gctggtacgg
     3361 gcggttgaga gtttcgaaaa atctgtaacg cctgctctac ttgaaggtta ccagggttca
     3421 tttttagaca agtttcaaga acttgaatcc gtctttcagt ggtcgcgcag gtttccacat
     3481 accaaaacca agcagggatg ttcttcggct cggtcttgat aatttcttgc agaatgcgct
     3541 gcgcctcaaa tttttgcccc tgacgaatga gttggatggc ttgatttaga tctgtcattc
     3601 gaactcccaa gaaaaagata tgcccatagc ttgtgttgac caagctatgg gcaggttaaa
     3661 acaactctgc taatttagcg gcaaacccaa ttccattaac tttgcctgag tggggtaacc
     3721 tttttcatcc cagccacgtt tttcatagaa ctctggcaac atcttgtcga gttctgctac
     3781 ttttcccttg gcaggaccat caggcaaagg ttcatgcaac atgcggtaag ggagcacatc
     3841 ccattcggga ccagaccctg ctttgatgag gaacatgcgc tcgagagtat aaacacggtc
     3901 ggcggccttc atcacttctt caggggtata acctgcacct gtggataaat tcatcatagc
     3961 tgccaaggtc tgaggccaga taaggcggtt cttgtcgaat acatagcgaa tggcaacgaa
     4021 tacgcacaac cctgaggagt cgatcaaggc aaacgaatct tggaagtgtt tgaccagttc
     4081 tggttttcct tccgttgaaa gcggattttc cttgatcggc acaccaaaga cttcttcata
     4141 cgccacatcg ccttccatat gtgacgcgcc cttgttggag gtggcataca acaagcccat
     4201 accttgcgag ccacgtggat catagccggg gaattcttgc ttacgagtcg tcactgccaa
     4261 ctcggggtga ccgtactttt ccgcaaggcg ataactgcct tgcgccagat cctgaccaaa
     4321 gcctttgtta taagccatca gcttgatcat ctcgatcatg gcatcatggt cgccgaactt
     4381 aagcggcata ccaatagttt cttcggtgag atagcccttt tcgtacattt ccattgccac
     4441 ggcaattgtc atgccggtgg tgatggtatc cataccgtat tcattgcaca gataatttgc
     4501 ttttaccaat gcagcaagat tgctcacacc gcaccccgta cctaaagatg aaatagtttc
     4561 atattcagga ccttctccct tgcctttata cggaccatcg ggcacttcgg tgaggcgccc
     4621 acatgaaagc ggacagcgat aacaaggggt atggcgaatc aaatattgat tcttcaaggc
     4681 atcgccatct acattgtgaa catcttcaaa agtgccttgt aaaaagtttc gcgtgggcaa
     4741 aatgccgagg gtgttggtca cttgtggcac atacgaagtg ccataaatgc gcatgttcga
     4801 gcccttcttt acgtcggcac caacattctt ggaggtctcc acgctgatgg cacgcatccc
     4861 ttcagaatca tgcagaggcg ggtttttatt tcccattgcc actacggctt tgagattctt
     4921 acttcccatc acagcaccca cacccgaacg agccgccgca cgatgtttat cgttcatgat
     4981 agctgccatc agtgcaagat tctcgcctgc aggtccgata catgcgactc gcgcttcatc
     5041 ggatgtttct gccttgagga tgtctgtcgt ttcgtgtgta tccttgcccc acacatgcgc
     5101 cgcggactta atttcgattt ggtcttcgtt caccaaaaca taaactggct ctgaggcttt
     5161 gccctcaaag ataaacatat cgaaccctgt gcgtttcatc cacgttggga attcaccacc
     5221 agagttagag tgtgcaagtg cgcccgtcag cggagatttg gttaccacca tgtatcggtt
     5281 gccggtcggg gcaggcgtgg ccgttagcgg accagtggca aagatgagca tgttctctgg
     5341 cgaaagcggg tccacggcag ggtccatttc tttgtacata taatggatcg cccatccgcg
     5401 cccaccgaga tagtcgcgtg cgatttgtgg gtctacatct tcagtggtta cttttcgagt
     5461 agtgaggttt acgcgtaaga ttctgaggtg ccagccgtac atgggaactc cttgaaaaag
     5521 tttgaacgtt gaaatgtttc aacgattaga accgagatta gccgccagcg attgcggaaa
     5581 atgctgtgac catgtcaccc tctcttaacg gggtatccat atccacggtg agaccgttca
     5641 agacgatcac gctttcatca ttaaggggaa tatcaaaccg agagatagca tcgtagactg
     5701 ttgtcccttc cgggacatct aactcaatga cgccatgttt atgccctgga gggagggcgt
     5761 cacgatagtt ggca
//
LOCUS       DAOQNJ010000098         5764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8111_length_5764_cov_7.753022, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000098 DAOQNJ010000000
VERSION     DAOQNJ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8111_length_5764_cov_7.753022"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1422)
                     /locus_tag="PLG52_04335"
     CDS             complement(112..1422)
                     /locus_tag="PLG52_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNB40705.1"
                     /translation="MNPSSIYQSRVTTAEEAIKQVKSGNRVFLTGNVSVPKVLLSALV
                     GHAPNINDVEVCQALTIGAADYVTEAMEGHLRVNSMFISANVRKAVQDGRADFTPVLL
                     SEFPLLFKRGILPVDVALIHVSRPDEHGFCSLGVEVGLTKSAAESAKIIIAEVNDQMP
                     RTLGDSFIHVSRLNYIVPVNYPIPEHKMAAEGDSDIVQGIAQHVASLIPDGATMQLGI
                     GSIPDAVLKFLMDKKDLGIHTELFSDGVIELVNAGVLTNARKSLHPGKIVAGFILGTQ
                     KLYDWVNDNPMVEMHPTEYVNDPFIIMQNERMVAVNSAIEVDLTGQVCADSIGPKLYS
                     GVGGQLDFIYGASRSKGGVPIIALPSTATMRDGTKVSKITAMLKPGAGVVTSRNHVHY
                     VVTEYGIADLYGKTIRQRAQALIKVANPIFHDELEKQAKELHYL"
     gene            complement(1419..2510)
                     /locus_tag="PLG52_04340"
     CDS             complement(1419..2510)
                     /locus_tag="PLG52_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNB40706.1"
                     /translation="MYIGIPKESRPFEYRVGLSPAGVEILSHLGHQVFVEHDAGASAG
                     FKDQDYEKAGGRIAFSAQEVFGRADFLLKVSRPIKQEMEWINDGAILAGFLHLASASQ
                     DQIDLLLEKKITALAYEQITDAQGGRAVLRPFSLICGAMVAQIAARWLQTNRGGKGIM
                     LSGVPGVPPAEVVIIGGGAVGTSAAQAMQNAGAHVTVLDKNMAALENIHDKLPGVVTM
                     MATQRNIERATAYADVVVGAVLVAGQRAPMVITREMVRAMKPRSVIIDVSIDQGGCVE
                     TSRPTMHDNPTFIEEGVVHYCVPNIASTVARTASHVFLNSAIPYITAIANTGIDAAMK
                     NDPSIEVAVNTHQGKLVNLVRLGGVEVKE"
     gene            complement(2569..3771)
                     /gene="rocD"
                     /locus_tag="PLG52_04345"
     CDS             complement(2569..3771)
                     /gene="rocD"
                     /locus_tag="PLG52_04345"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNB40707.1"
                     /translation="MNTQDFIKIEEQYGAHNYHPLDVVIEKAEGVWVYDVEGRKYLDC
                     LSAYSAVNQGHVHPEILNALLEQAKKVTLTSRAFRNDQLPLLYKELSEMTGYDMSLPM
                     NSGAEAVETAVKLARKWAYQVKKVPRHQAEIIVAGNNFHGRTVTIVSFSTEPLYRDDF
                     GPFTPGFVVVEYGNLDALEKAITPNTAAVLLEPIQGEAGVIMPPAGYLKKVAELCKKN
                     NVLYISDEIQTGLGRTGKLFAANHEDVRPDVVIVGKALAGGFYPVSAVLADKPILGLF
                     TPGEHGSTFGGNPLAAAVARASLKVLREEKLADRAAELGTYFVEQLSEIPSPHVKEVR
                     GKGLLIGVELKPEAGGARRFCEALKSKGILAKETHDNVIRFAPPLVIDKDTIDWALPA
                     IRETLNMP"
     gene            complement(3805..4731)
                     /gene="argF"
                     /locus_tag="PLG52_04350"
     CDS             complement(3805..4731)
                     /gene="argF"
                     /locus_tag="PLG52_04350"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNB40708.1"
                     /translation="MKDFIAISDYSPEEIEDLLDLAVKLKKQYFKKGNKPIFQGKVLG
                     MIFQKPSLRTRVSFDMAMRHCGGDALYLSPNEIGLGKRESIADIARVLSGYVQALMAR
                     TFDHAHVVELAKWADIPVINGLSDYNHPCQGMADALTIIEKFGKDSKGLNVSYVGDGN
                     NVTVSLMHVAAQLGWNMTVGTPEGYDVNPQAVEIAQNIARKTGSKLTFVRDVHEAVKN
                     AHVIYTDTWTSMGQEEETSKREQIFPPYQVNAKLVSEADKDVIVMHCLPAHRNHELTD
                     EVADGPHSVIFQQAHNRLHAQKAILARLFDVA"
     gene            complement(4809..5711)
                     /locus_tag="PLG52_04355"
     CDS             complement(4809..5711)
                     /locus_tag="PLG52_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB40709.1"
                     /translation="MANTPIVTIDLHFQGKTQAIASYLIRQGDAVVLIESGPGSTLSA
                     LEAGLAKEGLSPRDVTHVLLTHIHLDHAGAAGWLSRQGAEIYVHPIGAPHLLNPEKLI
                     ASATRIYGDRMDTLWGEFLPVAQEQLKTPNDAEEIVIGTMRFLPVNTPGHAEHHYSYV
                     FEDVCFSGDVGGVRIPGFQYLRAPMPPPELHFGRWRESLARLRGMKFAHIAPTHFGMF
                     DDVQWHLSTLDETLAAMEKWLETVMAQDPSIEKLREEFTLWMEEEGKAKNLSEDVLRA
                     YSLSNPVAMSADGLMRYWKKVRSA"
BASE COUNT         1468 a         1549 c         1304 g         1443 t
ORIGIN      
        1 cgtatgatag gtctaaaaca gaggaatgtc aagccggggc aaattattat gggtttaaca
       61 ataaaaaggg catggagtta cgcaaactcc atgccctttt tgggtactct tttaaaggta
      121 atgcagttct ttcgcctgct tttccagctc atcatggaaa atcgggttcg ccaccttgat
      181 cagtgcctga gcccgctggc ggatggtctt gccgtacaag tccgcgatgc catactctgt
      241 caccacataa tggacatgat tacggctcgt gaccacgccg gcacctggtt ttagcatggc
      301 agtgatctta ctcaccttgg tgccatctcg catggttgcg gtgctgggca aagcaatgat
      361 gggcacacca cctttggaac gagacgcccc gtaaatgaaa tctaactgcc cacccacacc
      421 gctgtatagc ttcggaccaa tgctatcggc acagacttgc ccagtaaggt ccacttctat
      481 ggcagagttg accgccacca tgcgttcatt ctgcataatg ataaacggat cattcacata
      541 ctccgtcggg tgcatttcga ccattgggtt atcgttgacc cagtcataaa gtttttgggt
      601 acccaagata aaaccagcca caatcttgcc cgggtgcaga gatttgcgag cattggtcaa
      661 cactcctgca ttgaccaatt cgatcacgcc gtctgagaat aactcggtat gaatcccgag
      721 atctttctta tccatcagaa atttcaacac agcatcaggg atggaaccaa tacccagttg
      781 catggtcgcg ccatcgggaa tcagcgaagc aacatgctgg gcaatgcctt ggacgatatc
      841 cgagtcacct tcggctgcca tcttatgttc agggatgggg taattgaccg gcacaatgta
      901 gttcaaacgg ctcacatgaa tgaaggaatc ccctaatgta cgcggcatct gatcattgac
      961 ttcagcaata atgatcttgg cagactcagc cgcagacttg gtaagcccca cttccacgcc
     1021 aaggctgcag aacccatgtt catctgggcg agagacatga atcagtgcca catccacagg
     1081 cagaatgcca cgtttgaata gaagtgggaa ttctgaaagt aatacaggcg taaagtctgc
     1141 gcgaccatct tggactgctt tacgaacatt ggcgctaata aacatggaat tgacgcgtaa
     1201 atgcccttcc atggcttcag ttacgtaatc ggcagcacca atcgtcaatg cctggcaaac
     1261 ttccacgtca ttgatattgg gagcatgtcc caccagtgca gacaaaagta ccttggggac
     1321 cgagacgttc ccggtcagaa agacgcggtt gcccgatttg acctgcttga tggcttcctc
     1381 tgcagtcgtc acacgggatt gataaatgga agatggattc attccttcac ctccacgcca
     1441 cccaaacgta caaggttgac taacttccct tgatgtgtgt tgactgcaac ttcaattgaa
     1501 gggtcgttct tcatggcggc atcaatacct gtgtttgcta ttgctgtgat gtagggaata
     1561 gctgagttca aaaacacatg gctggctgtg cgcgccactg tgctggcaat attgggaaca
     1621 cagtaatgca caacaccttc ctcaatgaaa gtgggattat cgtgcatggt cggacgggag
     1681 gtttctacac aaccaccttg atcgatactg acatcaataa tgaccgagcg cggtttcata
     1741 gcacggacca tttcgcgtgt gatcaccatc ggtgcgcgtt gacctgccac caaaaccgcc
     1801 cccacaacta cgtccgcata ggctgtggca cgttcaatgt tgcgttgcgt tgccatcatg
     1861 gttaccacgc caggcaactt atcgtggatg ttttccaacg cagccatatt cttatctagg
     1921 acagtcacat gcgcaccggc attttgcatg gcttgcgcgg cagaagttcc caccgcacca
     1981 ccaccgataa ttaccacctc ggcaggcggc acaccgggta cgccactaag cataatgccc
     2041 ttgccaccgc gattcgtctg cagccaacgc gccgcaattt gagcgaccat cgcaccgcag
     2101 atcaaactaa acggacgcaa aacggctcgt ccgccttgag catctgtaat ctgctcgtat
     2161 gccagcgctg taatcttttt ctccaagagc aggtcaatct gatcttgtga agcagaagcg
     2221 agatgtaaaa accccgcaag gatagctcca tcattgatcc attccatttc ctgcttgatc
     2281 gggcgtgaga ccttcaataa aaagtctgcc cgcccaaaaa cttcttgcgc agaaaaggcg
     2341 atgcgcccgc cggctttttc gtaatcctga tctttgaagc cagcactcgc ccccgcatca
     2401 tgctcaacga aaacttgatg ccccaaatga gacagaatct ccaccccagc cggagacagc
     2461 ccaactcggt actcaaacgg tctactttct ttcggaattc caatatacat ggttcctcca
     2521 taaaaagacc ctagaggtat ataaaccttt agggtctggt ttaatttatt acggcatatt
     2581 gagagtttcc ctgatcgcag ggagcgccca atctatcgta tctttatcaa taacaagtgg
     2641 tggtgcaaaa cgtatcacat tatcatgcgt ttctttagca aggatgccct tgctcttcaa
     2701 tgcttcacag aaacgacggg ctccgcctgc ttctggcttg agttccacac caatgagcaa
     2761 gcccttacca cgcacttcct tgacatgtgg actggggatt tcgctcaatt gctctacaaa
     2821 gtaagttccc aattctgcgg cacggtctgc taatttttct tcgcgcaaaa ctttcaatga
     2881 agcacgagcc accgcagcag cgagcggatt tccaccaaat gtggagccgt gttcaccggg
     2941 tgtgaacaag cccaaaatgg gtttatccgc gagaacagca gagacggggt aaaagccacc
     3001 agccaatgcc tttcctacga tcacaacatc cgggcggacg tcttcatgat tcgcggcaaa
     3061 cagtttcccg gtccgaccga gacccgtttg aatttcatcc gagatataaa gcacattatt
     3121 cttcttgcaa agctcggcaa ctttcttcaa gtatccagcc ggcggcatga tcaccccagc
     3181 ttctccctga atcggttcga gcaaaaccgc agccgtgttg ggcgtaattg ccttttcaag
     3241 cgcatcaagg tttccatatt caaccaccac aaaacccggc gtgaacggac cgaaatcatc
     3301 ccgatagagc ggttctgtgg agaatgaaac aatggtgacg gtgcgcccgt ggaagttgtt
     3361 gcccgctacg ataatttccg cctgatggcg cggcaccttc ttgacctgat acgcccactt
     3421 gcgagcaagc ttcaccgccg tctcaactgc ttctgcacca gagttcatcg gcagagacat
     3481 atcgtagccg gtcatttcag aaagttcttt ataaagaagc ggcaactggt cattgcggaa
     3541 agcgcgcgaa gtgagtgtga ccttctttgc ctgttccaac aatgcattca aaatttcagg
     3601 atgaacatgc ccttggttta cagcagaata tgcagaaaga caatcaaggt attttctgcc
     3661 ttccacatcg taaacccaca ccccttctgc tttttcgatg accacatcca atggatggta
     3721 attatgcgcg ccatattgtt cttcgatctt gataaagtct tgggtattca taaaatgggt
     3781 atcctctaaa aactatgaat atctttatgc gacatcgaat aaacgcgcca aaattgcctt
     3841 ctgcgcgtgc aagcgattat gcgcctgctg gaagatcacc gagtgtggac catccgctac
     3901 ttcgtcagtc aactcgtggt tgcggtgtgc aggcaaacaa tgcattacga tcacatcttt
     3961 atctgcttcg ctcaccaact tggcattgac ctgatacggc gggaagatct gttcgcgttt
     4021 ggaggtttct tcttcctgcc ccatgctggt ccacgtatcg gtataaatca catgagcatt
     4081 cttcactgct tcatgcacat cgcggacaaa agtcaacttg ctgcccgttt ttctggcgat
     4141 gttctgagca atttccaccg cctgcgggtt gacgtcgtag ccttcgggcg tgccaaccgt
     4201 catgttccaa cccagttgag ctgctacatg catcaacgaa accgtgacgt tattgccatc
     4261 gccgacataa ctaacattca aaccttttga gtctttgcca aatttttcaa tgatggtcaa
     4321 tgcatctgcc atgccctgac agggatggtt gtaatcgctc aagccattga tcaccggaat
     4381 atctgcccat ttggcgagtt ccaccacgtg agcatggtca aaggtacgtg ccatcaatgc
     4441 ttgaacataa ccagataaca ctcgcgcaat atctgcaatt gattcgcgct tgcccaaacc
     4501 aatttcgttt ggagaaagat acaaggcatc gccgccacaa tgtcgcatcg ccatatcaaa
     4561 agaaacgcgc gtgcgcaggc tcggtttttg gaagatcatt cccagtactt tgccttgaaa
     4621 gatcggtttg ttgccttttt tgaaatattg ttttttgagt tttacagcaa ggtcgagcag
     4681 gtcttcaatt tcttctggtg aatagtcaga aatcgcaata aagtccttca tgtgtgctcc
     4741 tcagggaaag tatatctttt ttagtctatt ttgccatgtt tcaagcatca cgcaaagaat
     4801 atgacaaatt acgcactgcg aactttcttc caataccgca ttagcccatc ggcactcatc
     4861 gccaccgggt tagagagaga ataagcccgc aacacgtctt cgctcaagtt ctttgcctta
     4921 ccttcttctt ccatccacaa cgtaaattct tcgcgcaact tctcaatgga cgggtcttgc
     4981 gccatcactg tttcaagcca tttctccatt gcagccagcg tctcatcgag ggtgctcaaa
     5041 tgccattgca catcatcaaa catgccaaaa tgggtcgggg cgatatgcgc aaatttcatt
     5101 ccgcgcaaac gcgccaaact ttcacgccaa cgcccaaaat gcaactctgg tggtggcata
     5161 ggcgcacgca aatattgaaa gcccggaata cgcaccccgc ctacatcccc actgaaacat
     5221 acatcttcaa aaacatagga ataatgatgt tctgcatgcc ccggtgtatt gaccggaagg
     5281 aaacgcatag taccgatgac aatctcctcc gcatcattcg gagttttgag ctgttcctgc
     5341 gctacgggca gaaactcacc ccaaagtgta tccatcctat ctccgtaaat acgggtggca
     5401 ctggctatca gcttctcggg gttcaataaa tgcggagccc caatggggtg tacataaatc
     5461 tccgcgccct gacgagacaa ccaacccgcc gcccctgcat ggtcaagatg aatgtgcgtg
     5521 agcagaacgt gggttacgtc tcggggagat aagccctctt tcgccaaacc agcttcgagc
     5581 gcagaaagag tcgagccggg tccgctttcg attaagacaa cagcatcgcc ttgtcgtatc
     5641 aaatacgagg cgatggcttg agtcttgcct tgaaaatgga ggtcaatcgt tacgataggg
     5701 gtgttcgcca tgcatgcaat tcctgtagtt tgaaattaga ggatatgtct gccagcgcct
     5761 gcgg
//
LOCUS       DAOQNJ010000099         5739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8170_length_5739_cov_6.775862, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000099 DAOQNJ010000000
VERSION     DAOQNJ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8170_length_5739_cov_6.775862"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1103
                     /locus_tag="PLG52_04360"
     CDS             201..1103
                     /locus_tag="PLG52_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB40710.1"
                     /translation="MNNKRNILLAVSVLVWASLACKALSPSETADLPQETGSTEESNP
                     LLPSDSSSPATEEASAQSAGLYDGSWVGTNTVDNKEILFRVENNEIVSVALNYTGESN
                     GCDYHGALSTGSETGGIDPISIASESFSAVITSVNDELTFSGTFSSETEASGTLLIKS
                     SAEGICGEYEKEVSWTATKGSSAEAETSQESSGTLAQGDPVEIVTQFFDAVNAGDLNS
                     AVAVTDENVMFTIGTSVTKFSSSELESYFEANQGVTYQLSDASSMAAGTMVQFKVKAS
                     DGTTYSYCQAFVTDGKITMMSWMP"
     gene            1219..2751
                     /gene="ftcD"
                     /locus_tag="PLG52_04365"
     CDS             1219..2751
                     /gene="ftcD"
                     /locus_tag="PLG52_04365"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNB40711.1"
                     /translation="MTNQLIECIPNFSEARRPEVIDQIVAAIQAVGEVKLLDRSSDLD
                     HNRTVLTFAGSAAGVEEAAFRAIQTASELIDLDQHTGEHPRIGATDVVPFVPLSGASM
                     EECIAIAKRLAERVGKELNIPVYLYEAAATKPERTNLENIRKGQYEGLKTEIEKPERQ
                     PDFGPNTLPKAGATVIGARNPLIAFNVYLTTDDVSIAKKIAKAVRQSSGGLRYVKGLG
                     LLVEGRAQVSMNLTNFRETPIARVVEFIRREAERYGVGIHHSELVGLIPQEALVDAAV
                     WYTQLDAFSPEQVLESRLFSTPTASASNALKPASFIEELAAPTPTPGGGSAGAYAGAM
                     GAALVAMVAGVTIGKKKYAEVEAEMQAIRVVAENLRKELTQAVDDDAASFEVLMAKFK
                     MPKETEEQKSAREAAITQATLNAAHIPLHVSEHAVKVMELALKCAKSGLQSAISDAMS
                     GFAMARASLTAAGYNVRININSLEDKSLGDRMLSELAELEKAADKLESEIRTVMKERG
                     GI"
     gene            2814..3680
                     /locus_tag="PLG52_04370"
     CDS             2814..3680
                     /locus_tag="PLG52_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB40712.1"
                     /translation="MDKKLSRRDFLRILKLFGLGAFFAYETRYVSAIEPSWLEVTQVA
                     LELQGLPKAFHNYRILQLSDIHMGGWMNRERFSAVLDVVEQQSFDLLVITGDFVIGGR
                     SWTENLDLAAIDFIELMKPVTKRRLTLGVMGNHEYKTDPVLARKMLAEAGVMELKNDV
                     HLLEKDGEALYIAGLDDISKHQQSLDQVTQKLPALAPTILLVHEPDYATVTSKTKRFL
                     AQLSGHSHGGQVVFPLVGPLVLPQWGRRFPAGLYQINDMFLYTNRGVGMTPPYVRFGC
                     RPEITIFTLNQG"
     gene            3713..5275
                     /locus_tag="PLG52_04375"
     CDS             3713..5275
                     /locus_tag="PLG52_04375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB40713.1"
                     /translation="MLPPNVLYSAILLAAGVGCLVVAVAIWLKRREAIGAKSLFVFLL
                     GLSWWDITYGIFWADIPGPNPYFWLDITLFGAFVVPTAFLIFALEYSNSQKWLGRPFI
                     IAAIIEPILISILLCTDRWHNLYFGGVRALNTTMLVEMGPAAWANVVYSYLLILIGVL
                     VLAVTLYRSRGVYRMQVTFILAATVTPWVIHILFLSIGDYALLPDADVTPFIFSMTAL
                     LIAYALIRYRLLDVVPIARSILIESMSEGVCVLDSQNRIVDINQTARKALSSEFFIGQ
                     PVEQAFKCWIDIVSHFFDQETAQTEVVIDDRYLDLRISPVYDKNQRLLGKLVIWRDIT
                     ELKNIQLKLQQLATIDELTLAFNRRHFMEIAEVQFSQALRQNQPFALALIDLDYFKGI
                     NDKYGHPAGDQVLAEFSRMFMENIRKFDVFGRLGGEEFALLMPNTDMHFAYHASERLR
                     FMITNTPIPINGGDVIVTFSLGVAMLQGAQDTLSAVFRRADQALYAAKNSGRNQVVIW
                     EQGLEAVASKQA"
     misc_binding    5322..5572
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1415 a         1349 c         1448 g         1527 t
ORIGIN      
        1 aacatctttt ttgttgcctt aaaaggtagt agtgataaat aatctttctt gaatcttgcc
       61 atgctctcac tagcattggt aaaagacggc tctataaaat ccccttacct ttaggtgata
      121 ttcaaaccca caggcttcgg taaaatctgc cctgctttag tcgtattcta aaaacttatc
      181 ttttattctt ggagcctttc atgaacaaca aaagaaacat cctcttggca gtcagtgtgc
      241 tggtctgggc atcgcttgct tgtaaagcct tgtcgccttc agagaccgcc gacctgccac
      301 aagagaccgg tagcaccgaa gaaagcaatc cgcttttacc ttctgattct tcctcgcctg
      361 ccaccgaaga agcctctgcg caaagcgccg gtttgtacga tggcagttgg gtcggcacaa
      421 acacggtgga taacaaagag atcctgttcc gcgtggaaaa caacgagatc gtttcggttg
      481 ctttgaacta cacgggcgaa tcaaacggct gtgattatca cggcgcgctc agcaccggtt
      541 cagaaaccgg cggcattgac ccgatctcca tcgcgagcga gagtttctct gcggtcatca
      601 ccagcgtcaa cgatgaactg acctttagcg gaaccttcag ctctgaaacg gaagccagcg
      661 gcaccctgct catcaaatcg tccgcagaag gcatttgcgg agagtatgaa aaagaagttt
      721 cttggaccgc caccaaaggc tccagcgcag aagccgaaac cagccaagag agcagcggca
      781 cgctcgccca aggagacccg gtcgagatcg tcactcagtt ttttgatgcc gtcaacgccg
      841 gtgatcttaa ctctgccgtg gctgtgaccg atgaaaatgt gatgttcacc atcggcacca
      901 gcgtgacgaa gtttagcagc agcgaactcg aaagctattt cgaagccaat caaggcgtaa
      961 cctaccaact cagcgacgct tcttccatgg cggcgggcac catggtgcag ttcaaggtca
     1021 aagccagtga cggcaccacc tattcctact gccaagcctt tgtcaccgac ggcaagatca
     1081 ccatgatgtc ttggatgcca taaatcaatc aaaagaccga aacaacctca ccaagcggtg
     1141 aggttgtttt ttttctcctg aatatctttt ataatggatg catacccaac taacgaaact
     1201 gcgagactat caaactccat gaccaatcaa ctcattgaat gcattcccaa cttttccgaa
     1261 gcgcgtcgcc ccgaagtgat cgaccagatc gtcgccgcca ttcaagccgt gggggaggtc
     1321 aaactgctcg accgctcctc tgacctcgac cacaatcgca ccgtgctaac ctttgccggg
     1381 tctgccgcag gcgtagaaga agccgccttc cgtgccattc aaaccgcctc cgaactgatc
     1441 gacctcgacc agcacaccgg cgaacatccg cgcatcggcg caaccgatgt ggtgcctttt
     1501 gttccgctca gtggcgcaag catggaagaa tgtatcgcca tcgccaaacg ccttgctgag
     1561 cgcgtgggca aagaactcaa tattcctgtt tatctttacg aagccgccgc taccaaaccc
     1621 gaacgcacca accttgagaa catccgcaaa gggcaatacg aaggcttgaa gaccgagatc
     1681 gaaaaacccg aacgccagcc agattttggt cctaatacac ttccaaaagc gggcgccact
     1741 gtcatcgggg cgcgcaatcc gctgattgcc ttcaacgtct acctcacgac cgatgatgtt
     1801 agcatcgcga aaaagattgc caaagccgtg cgtcagtctt cgggcggtct gcgttacgtc
     1861 aaaggtctcg gtctactcgt ggaagggcgc gcgcaagttt cgatgaacct taccaacttc
     1921 cgcgaaacgc cgattgcgcg tgtggtcgaa ttcattcgcc gcgaagccga acgttatggc
     1981 gtcggcattc atcacagcga attggttggt ttgattccac aagaagccct tgtggatgcc
     2041 gcggtctggt acacccagtt ggatgctttc tctcccgaac aggttctcga gtccaggctg
     2101 ttttctactc ccaccgcttc cgcctccaac gcgctcaagc ctgcttcttt cattgaagaa
     2161 ttagccgcgc cgacaccgac gcccggcggc gggtccgcgg gggcttatgc gggtgccatg
     2221 ggcgctgcct tggttgccat ggtggcaggc gtgaccattg gcaagaagaa atacgctgag
     2281 gtcgaagctg aaatgcaagc catccgtgtg gtggcagaga atcttcgtaa agagcttacc
     2341 caagctgtcg atgacgatgc ggcatccttt gaagtcttga tggcaaaatt caaaatgccg
     2401 aaagagaccg aagagcaaaa gtccgctcgt gaggcggcta ttacgcaggc aaccttgaac
     2461 gccgcccata ttccactgca cgttagcgaa catgcagtca aggtcatgga gcttgctctt
     2521 aaatgcgcca agtctggttt gcagagcgct attagtgatg ccatgtctgg ctttgcgatg
     2581 gcacgcgcat ccctgacggc tgccgggtac aacgtccgca ttaacatcaa ttcgcttgaa
     2641 gataaatcat tgggcgaccg tatgttgagc gagttggcag agttggaaaa ggcggcagat
     2701 aagctcgaaa gtgagattcg aaccgtgatg aaagaacgag gcgggatcta aatcccgcct
     2761 ttttattttt aaggtgggtt ttagtttcgt ttgagatgat tttagaagca gttatggata
     2821 aaaaattatc aagaagagat tttctaagga ttctcaagct ctttgggcta ggcgcttttt
     2881 ttgcatatga aacccgatat gtaagtgcta ttgaaccttc ctggcttgaa gtgacacagg
     2941 ttgctttgga gctacagggg ttaccaaagg cttttcataa ttatcgtatt cttcaattga
     3001 gcgatattca tatgggtggt tggatgaatc gagaacgctt ttccgctgtt ttggatgtgg
     3061 tggagcaaca aagttttgac ttactggtga tcacgggaga ttttgtaatc ggcggacgtt
     3121 cttggacgga aaacctggat ctagctgcta tagattttat tgagcttatg aagcctgtta
     3181 caaagcgccg ccttaccttg ggtgtgatgg gaaatcatga atataagacg gatccagtcc
     3241 ttgctcggaa aatgcttgct gaggcagggg tgatggagct taagaatgac gttcatcttt
     3301 tggaaaaaga cggagaggct ttgtatattg ctggcttgga tgatatctcg aagcaccaac
     3361 aaagcttgga tcaagtaacc cagaaactac ctgcgctcgc tcctactatt ttgttggtac
     3421 acgaacctga ttatgcaacg gtaacctcta aaacaaaaag attccttgct caactttcag
     3481 ggcattccca tggcgggcag gtcgtgtttc cgttggtggg tccgcttgtg ttaccacaat
     3541 ggggcagacg ttttcccgct gggttgtatc aaattaacga tatgtttctt tataccaacc
     3601 gtggagttgg gatgacgcca ccttatgtcc gctttggttg ccgacctgag attacaatat
     3661 ttaccctaaa tcagggatga tattcaagtt gggggtattg tataatttga gtatgcttcc
     3721 accgaatgta ttgtatagtg caattttgtt ggcggcaggt gtggggtgtt tggtagttgc
     3781 cgttgcaatt tggctgaaac gccgtgaagc cataggggcg aaatcgcttt ttgtattcct
     3841 attagggcta agctggtggg atataaccta tggcatcttt tgggcagata tacctggacc
     3901 aaacccatat ttttggttag atatcacctt gtttggtgct tttgttgtgc cgaccgcgtt
     3961 tttgattttt gcattggaat actctaattc gcaaaagtgg ctggggcgac cttttattat
     4021 tgcagcgatc atcgaaccca tcttgatttc catcctgctg tgtacagacc gttggcataa
     4081 cctttatttt ggcggggtgc gtgctttgaa cacgaccatg cttgttgaga tgggaccggc
     4141 tgcctgggca aatgttgtct actcgtatct tctaattctt attggcgtgc ttgtattggc
     4201 tgtaacgttg tatcgttcgc gcggggtcta tcgtatgcag gtgacgttta tcttggcagc
     4261 gactgtgacg ccctgggtaa ttcacatcct ctttctttcc attggtgatt atgctctttt
     4321 acccgacgcc gatgtgacac catttatttt ctcaatgaca gcgttactca ttgcatatgc
     4381 tcttattcgt tatcgtttat tggatgtcgt tccgattgct cgcagtattc tgatcgagtc
     4441 tatgagcgaa ggggtttgcg tcttggattc acagaaccgc atcgttgaca ttaatcagac
     4501 agcacggaag gcgctctctt cggagttctt tattggacag ccagttgaac aagcgttcaa
     4561 gtgctggata gatattgtct ctcatttttt cgatcaagaa acggcgcaga cggaggttgt
     4621 gattgatgat cgatatcttg atctacgtat ttcgcctgtg tatgataaaa atcaacgcct
     4681 tttagggaag ttggttatct ggcgtgatat tacggaatta aagaatatac agctgaaact
     4741 tcaacaactt gctaccatcg atgaattgac actggcattc aatcgccgac attttatgga
     4801 aattgccgaa gtgcagttta gtcaagcact tcggcaaaac caaccctttg ccttggcgtt
     4861 gatcgatttg gattacttca agggaataaa tgataagtat gggcaccctg caggagacca
     4921 agttttagct gagttttcac gcatgttcat ggagaatatc cgcaaattcg atgtgtttgg
     4981 gcgccttggc ggggaggaat ttgctctgct tatgccaaat accgatatgc attttgcgta
     5041 tcatgcttct gaacggctgc ggtttatgat taccaatacg cctattccga tcaatggcgg
     5101 ggatgtgata gtaactttta gtttgggtgt tgctatgctg cagggcgcac aagatacgct
     5161 ctctgccgtt ttccgtcggg cagaccaggc gctctatgcc gcgaagaatt ctggcagaaa
     5221 tcaggtagtg atatgggagc aagggctgga agcagtagcg tcaaaacagg cttaaactca
     5281 cgccttgctc attccctgcg gcttgtgtat aatgccgcta tctaaaagcg tggatgggaa
     5341 cgagtaaacc gttcaagacg ggcagcgagt ccgaggtcag gtgtgaagcg gatacgttca
     5401 agacggttga aaatcctccc ggagctgtga actgaaatca gaaaattctg agagtaggga
     5461 atccgaaatt gttcttcgtt atcagaacag ggcgatggtt gtcgtccaat gagtgttcat
     5521 cttcggatga aaatcagagt ggtaccgcgt gagccttgct ctcgtctctg ttatgagatg
     5581 agagtttttt attttaattt caccgtcatt gcgtggatga tgacagaaac aaggagtaag
     5641 aatgttcaaa gcagtcaacc caaaactcga tgtgccttcg atggaagata acgtcctcaa
     5701 aatgtggacg aagcaggaga tcttcaagaa gaccgttga
//
LOCUS       DAOQNJ010000100         5713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8215_length_5713_cov_9.585543, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000100 DAOQNJ010000000
VERSION     DAOQNJ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8215_length_5713_cov_9.585543"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1221)
                     /locus_tag="PLG52_04380"
     CDS             complement(<1..>1221)
                     /locus_tag="PLG52_04380"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HNB40714.1"
                     /translation="KLILVTGATGYVGGRLVPKLLEAGYRVRCLVRDVSRLQGRAWSK
                     KVEVVAGDALNLDELASAMKDVSVAYYLMHGKQGGKDNAERDLKAARNFAQAAEDEGI
                     EQIVYLGELVDPTANLSPYLRSRHETGFVLRHGKVPVTEIRAGMIIGSGSALFEMIRY
                     LTEREPVLTCPAWFFSQAQPIAIRDVLSYLVDTLKTPDVIGRVIEVGGPTRLTYADML
                     LGYAKERNLRRWLIRTPFYAPRLSAYWVHMLTPIHWRVVAPLIEGLSAELIVRDETAK
                     KLFPHIQPIDFQTAVHLALGRVQRDNVETSWADALVTEAGDVKPYVFTVEDGLFIERR
                     QVVLDLPAETVFRSYTGIGGARGWMYMDWAWTIRGWMDKAIGGVGLRRGRRHPDEINT
                     GEALDFWRVELVEK"
     assembly_gap    1222..1321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1462..3849
                     /locus_tag="PLG52_04385"
     CDS             1462..3849
                     /locus_tag="PLG52_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNB40715.1"
                     /translation="MPLLWISLSFLTGIVIAAALPLPTWAWVLIALTFFLLFIFLRRS
                     QFATRFSFLNTFPFIPLLPLFLFLGSAYYQARQPKLDAFHVAFYNDRTYDLLITGYIE
                     DAPDYRDSYTNLILNVEAVDSGDGDLPVSGRLLVRVEPNETYEYGERIRLRGKLKTPP
                     ENEEFSYRDYLARQGIHSYMTIAEVTRLPGNDGNPIYKLIYGFKEQLVSHTYKIFPDP
                     EASLLSGILFGVDTGLPKKLQDDFKTTGTAHIIAISGFNIAIIAGLFFRFFKWTLKNE
                     RLGAVFAVIFVFLYAFLVGGDPAVIRAAIMGSFSLFAKQVGRRNAGMNTLAVVAALMA
                     LWNPLTLWDVGFQLSFFATLGLILYAEPLSNITVRFFEKVTQQEAGAIANIINDNVML
                     TIAAQIMTIPLMLYYFHRLSLISFIANPFILPVQPAVMILGGLAVFVSLLLQPIGQLL
                     AWVAWPFAGYTIRVVEFFAKTPNGVIVFGDVPLWFIYMTYTSLLLVTFNWSAIQNWFK
                     SAASSLRAIALTLAFSIAFICMVTFWSASAKSGDGQFHIIFLEVGSADAILIQTPEGR
                     NVLINGGASASELSDELGRRLPFFTRKLDWLIIAATDEDQLAALPRVVERYEPENVLW
                     SGNVQASFSARTLDEYFAEKEIPVSLAEAGQRLELGDGAFIEVQATGPRGSVLLVEYG
                     SFRALLPIGVSEGTFESLEFGNAIGKVDVLLLADSGFAPSNPPDLLENLNPQLIVLDV
                     AAGDPDGLPSQDVLDMLEGYSLLRTDRNGWVDVRTDGTEMRIQVERGEDTVKSEP"
     gene            3883..4272
                     /locus_tag="PLG52_04390"
     CDS             3883..4272
                     /locus_tag="PLG52_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB40716.1"
                     /translation="MSDLLKITKTEGKVTILHLEGVLDGQTEKGFVDAAHAEFEAGKT
                     TLLLDFGKLDIITSAGLRALQNLYKKFTPEEEIQAWNAEHQGEVHKAKNLRVAQASSE
                     VTYILSITGFLQSLYVYADIQEALDSF"
     gene            4276..4980
                     /locus_tag="PLG52_04395"
     CDS             4276..4980
                     /locus_tag="PLG52_04395"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNB40717.1"
                     /translation="MGYLLLEGGAEFGGRMSEPDLRAMELAGGFEAPIAILPTAAAPD
                     HNHKRAGNNGIRWFQSLGAKHVFSVDVIDAQSANDPKLADQLRNSKLIYLLGGFPRYL
                     GETLKGSACWSAAMEAYAKGAILAGSSAGAMVLCEHYYDPYERKLLAGLSLVPNACVL
                     PHHNNFGKSWAKQLKQMLPDITLIGIDERTGMLSTDALGTEVQWGVYGGGEVTLYRSE
                     ATTGYERGASFQLKIS"
     gene            5064..>5713
                     /gene="msrP"
                     /locus_tag="PLG52_04400"
     CDS             5064..>5713
                     /gene="msrP"
                     /locus_tag="PLG52_04400"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNB40718.1"
                     /translation="MSHRFKSVPVRSSEITPKEVYLSRRDFIKAAGVVAGSMALAACA
                     PESSATPAAQFNGPVLNDELGDPTNTFEEITNYNNYYEFTTNKEAVAPMAKDFTTTPW
                     TIEVSGMVSKPKTFGLEDLLSMFPQEERIYRLRCVEAWSMVIPWNGFPLASLLKAVEP
                     TSNAKYVRFETIYRPEEMKGQSSPFYPWPYQEGLRLDEAMNDLTLLATGIYGETILP"
BASE COUNT         1379 a         1492 c         1325 g         1417 t
ORIGIN      
        1 atttttccac caactccaca cgccagaaat ccagcgcttc accggtgttg atttcgtcgg
       61 gatgacgtcg tccacggcgc aaaccaacac cgccaatggc tttatccatc caaccgcgga
      121 tcgtccacgc ccaatccata tacatccacc cgcgagcacc gccgatgcca gtataagaac
      181 gaaaaaccgt ttcagcagga agatctaaca ctacttggcg gcgttcgatg aacaagccat
      241 cttccacagt gaaaacatac ggcttgacat cccctgcttc cgtgacgagt gcatctgccc
      301 aactggtttc cacattgtca cgttgaacac gcccgagcgc gagatgaaca gccgtttgaa
      361 aatcaatcgg ttgaatatgc gggaataatt tcttcgccgt ctcatcgcgg acgatcaact
      421 ctgcgcttaa cccttcaata agcggtgcga ccacacgcca gtgaatcggt gtgagcatat
      481 gcacccaata agcagataac cttggggcgt agaagggagt gcgaatcagc caacggcgga
      541 gattccgctc tttggcatac cccagcaaca tgtccgcata ggtgaggcgc gtgggtccgc
      601 ccacttcgat gactctgcca atgacatctg gcgtcttgag cgtatccaca agataggaga
      661 gcacatctcg aatggcaatc ggttgtgctt gagagaagaa ccaagctggg caggtcaaca
      721 ctggttcacg ctcggtgaga taacgaatca tttcaaagag cgccgacccc gagccgatga
      781 tcataccggc gcgaatttct gtcacaggaa ctttgccatg ccgcaacaca aaccccgttt
      841 catggcgcga ccgcaagtac ggcgaaagat tcgctgtggg gtctaccaac tcaccgagat
      901 aaacaatttg ctcaatgcct tcatcttcag cggcttgcgc aaaatttcgt gcggctttca
      961 ggtcgcgttc agcattgtct ttgccgccct gcttgccatg catcagataa tacgcaaccg
     1021 acacatcttt catcgcggag gcaagttcat ccagattaag cgcatcgcct gccaccactt
     1081 ctactttttt cgaccaggca cgcccttgta aacgagacac atctcgcaca agacaacgca
     1141 cgcgataccc ggcttcaaga agtttaggaa caagacgccc gcccacataa ccggttgcac
     1201 cggtgacaag gatcaatttt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 ngattctcaa gaatgggaaa cctttcatgg tccaactctg gtgatgccca tccacaaatc
     1381 catgcaggaa tggcgtcatg gcgaattgat tcacctacac ggtatgccgt ggtgatgact
     1441 aaactaaagt agaatccttt catgccctta ttgtggatct ctttatcttt cctaacggga
     1501 attgtcattg cagcagcact ccctctcccc acttgggcat gggtcttgat cgcgcttacc
     1561 ttctttcttc tttttatctt tcttcggcgt tctcaatttg caactcgttt ctcattccta
     1621 aataccttcc cattcatccc gctcctccct ctctttcttt ttcttggttc tgcttattat
     1681 caagcccgcc aacccaagtt agacgcgttt catgtcgcct tctacaacga ccgcacttat
     1741 gacctgctca tcacaggata catcgaagat gcgcccgatt atcgtgattc ctataccaac
     1801 ctcatcctca acgtcgaagc cgtggatagc ggcgatggtg acctaccagt ttcgggtcgg
     1861 ctgctggtgc gtgtggagcc aaacgaaacc tacgaatatg gcgagcgtat ccgtctgcgc
     1921 ggaaagctga aaactccgcc cgagaacgaa gaattttcgt atcgcgatta tctcgcccgt
     1981 cagggaatcc attcgtatat gacgatagcc gaagtgactc gtctccctgg caacgatggc
     2041 aaccccattt acaaattgat ctatggcttc aaagaacaac tggtaagcca cacctataaa
     2101 atttttccag acccagaagc ctcactgctc tctggcattc tcttcggcgt agatacgggt
     2161 ctgcccaaga aactacaaga tgacttcaaa accacaggca cggcgcatat catcgccatt
     2221 tctgggttca acatcgccat cattgctggt ctattcttcc gcttcttcaa gtggacgctc
     2281 aaaaacgaac ggctcggcgc agtattcgcc gtcatctttg tttttttata tgcctttctc
     2341 gtaggcggtg acccagccgt gatacgcgcc gccatcatgg gcagtttctc gctctttgcc
     2401 aaacaagttg gcaggcgcaa tgcgggcatg aatacgttgg ctgttgtggc tgcactcatg
     2461 gcgttatgga atccgctcac cttatgggac gtagggtttc aactctcctt cttcgcaaca
     2521 cttgggctga tcctatacgc tgaaccactc tcaaacatca cagtccgctt ctttgaaaaa
     2581 gtcacccaac aagaagccgg cgccattgcg aatatcatca acgataatgt catgctcacc
     2641 atcgccgcgc agatcatgac tatccctttg atgttatatt attttcatcg cctgtctttg
     2701 atctcattca tcgcaaaccc gttcattctg cccgttcaac cagcggtgat gattctgggt
     2761 ggtctggcag ttttcgtcag cttgcttctt caacccatcg gtcaactcct tgcatgggtg
     2821 gcatggccct ttgctggtta caccattcgc gtggtggaat ttttcgccaa aacaccaaac
     2881 ggtgtgatcg tattcggaga cgtccctctt tggtttattt atatgacata cacctcgctt
     2941 cttctcgtga cctttaactg gtctgccatc caaaactggt tcaagtctgc ggcttcatct
     3001 cttcgagcca tcgcgttgac tttagccttt tccatcgctt tcatctgtat ggtcacattt
     3061 tggagtgcct cggcaaaatc tggcgatggt caatttcaca tcatctttct cgaagttggt
     3121 tccgcagatg ctatcctcat tcaaacccct gagggacgaa acgtccttat caatggcggt
     3181 gccagcgcct ctgaactttc agatgaactc ggtcgccgtt tgcccttttt cacccgcaaa
     3241 ttagattggc tcattattgc cgctacagat gaagaccaat tggctgccct cccccgcgtt
     3301 gtagaacgat acgagccaga gaacgtgcta tggagcggaa acgtccaagc ttcgttctct
     3361 gcccgtacct tggatgaata ctttgcagag aaggaaatcc ccgtcagctt ggcagaagca
     3421 ggtcaacggc ttgagctggg tgatggagcg tttatcgagg tgcaggcaac gggtccgcgt
     3481 ggaagcgtgt tactcgtcga gtatggcagt ttccgcgcct tgttgcccat cggcgtcagc
     3541 gaagggacat tcgagtcgct tgaatttggc aatgccatcg gcaaagtgga tgtgctttta
     3601 ctcgccgatt caggctttgc tcctagcaac ccgcctgacc tattggaaaa tttaaatccg
     3661 caactgattg ttttggatgt tgcggctggt gaccccgatg gtttaccttc gcaagatgta
     3721 ttagacatgt tggaaggcta ttctctgctt cgtacggacc gcaacgggtg ggttgacgtc
     3781 cgcaccgatg ggacagaaat gcgtattcaa gtagaacgag gcgaagacac tgtaaaaagc
     3841 gaaccgtaat ttttagcgta catcaaaacc aaggagagag agatgtctga tttattgaag
     3901 atcacaaaga ccgaagggaa agtcaccata ttgcatttag aaggggtatt agacggacaa
     3961 actgaaaagg ggtttgtgga tgcagcacat gctgagtttg aggctgggaa aacaaccctg
     4021 ctactcgatt ttggcaagct ggatatcatc accagcgccg ggttacgtgc gttgcaaaat
     4081 ctttataaaa aatttactcc tgaagaagaa attcaagcat ggaatgctga acatcaaggc
     4141 gaagttcata aagccaaaaa tctcagggtt gcgcaggctt cttcagaagt gacgtacatt
     4201 ctcagcatta ctggcttttt acaaagcttg tatgtctatg ctgatattca agaagcattg
     4261 gattcttttt aggcgatggg ctatctttta ctggaaggcg gcgcggagtt tggcgggcga
     4321 atgtctgagc cggatctgcg cgccatggaa ttggcaggtg ggttcgaagc gccgatcgct
     4381 attctgccca ctgccgccgc gccagaccac aatcacaaac gagcggggaa caatggcatt
     4441 cgctggtttc aaagcctggg ggcaaagcac gttttctctg tggatgttat tgatgcacaa
     4501 tctgccaatg accccaagct cgccgaccag cttcggaatt caaaattaat ttacctgctc
     4561 ggcggctttc cccgttacct cggcgagacg ttaaaaggca gtgcctgttg gtctgccgcc
     4621 atggaagcct atgccaaagg cgcgatcctt gccgggagca gtgctggagc catggtgctg
     4681 tgtgaacatt actacgaccc ctatgagcga aaacttttag cagggctgag tctggttccg
     4741 aatgcttgcg tcctccccca tcacaataat tttgggaaaa gctgggcgaa gcagttaaag
     4801 caaatgctgc cagatatcac acttatcgga attgacgagc gtacggggat gctgtcaaca
     4861 gatgcgctcg gaacagaagt acaatgggga gtctacggcg gcggtgaggt gacactttac
     4921 cgaagcgaag caactaccgg ctacgaaaga ggcgcaagtt tccaactgaa gatttcctaa
     4981 gcaaattatc atctgtccgt tagaaagttt ttttagaatc agtgtgtcca ttaaagtaaa
     5041 tcattgaacg aggagagacc tccatgagtc atcgttttaa atctgtacct gtccgttctt
     5101 ctgagatcac ccccaaagag gtgtatctct ctcggcgtga ctttatcaaa gctgcgggtg
     5161 tggttgctgg cagtatggca cttgccgctt gcgccccaga atcctctgca accccagcgg
     5221 ctcaatttaa tggtcctgtt ctgaacgatg agctgggtga ccccaccaac acttttgaag
     5281 aaattaccaa ctacaacaat tactacgagt tcaccaccaa caaagaagcc gtagcgccca
     5341 tggcaaaaga cttcaccacc acgccgtgga ccatagaagt aagcggtatg gtaagcaagc
     5401 cgaagacttt tggtttagaa gacttgcttt caatgttccc acaggaagag cgcatttatc
     5461 gcctacgctg cgtagaagcc tggagcatgg tcattccgtg gaatgggttt ccgctcgcaa
     5521 gtttgttgaa agccgttgaa cccacgtcca atgcaaagta tgtgcgcttt gaaaccattt
     5581 atcgccccga agaaatgaag ggacaaagca gcccgttcta cccatggccc tatcaagaag
     5641 ggttacgcct cgatgaagcc atgaacgacc tgaccttgct ggcaactggt atttacggtg
     5701 aaaccattct ccc
//
LOCUS       DAOQNJ010000101         5704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8241_length_5704_cov_9.955921, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000101 DAOQNJ010000000
VERSION     DAOQNJ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8241_length_5704_cov_9.955921"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2572)
                     /gene="gyrA"
                     /locus_tag="PLG52_04405"
     CDS             complement(<1..2572)
                     /gene="gyrA"
                     /locus_tag="PLG52_04405"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNB40719.1"
                     /translation="MELGIVRKIDIDSEMQQAYLDYAMSVIVSRALPDARDGMKPVQR
                     RILYAMYDMGIRSDSPYKKSARIVGEVLGKYHPHGDSSVYEAMARLAQDFSMRTMLVD
                     GQGNFGSVDGDPPAAMRYTEARLTSAALDILNDLNKNTVDFTANFDDTLEEPSVLPAA
                     IPNLLVNGATGIAVGMATSIPPHNLSEVVDALVFMLERWEKLDDIDVEQLMKFVKGPD
                     FPTGGLIIEEKGDEGIESAYGSGRGRITVQAKAHIEEMERGKSRIIVSELPYMVNKAS
                     LIERIAELARDGHLEGLSDLRDESDRQGMRIVLELTKNADPNFVLRDLYKRTPMQTTF
                     SINLLALVDGEPRTLTLKQALRVYLEHRLTVIKRRAEFDLEKAKARAHILEGYLVALK
                     NLDEIIELIRSAPDVETARAKLMKRYKLTEIQANAILDMQLRRLAALERKKIETEYKE
                     VTAIIKELTTLLKTPKLMRGVVADELQKVKAAYGDRRRTQVVSVKEGGTAKALTTNDL
                     LPQQTVWVGVTDDGLVSRTADDKQPKHSGNDAPRFLVKASTTDTVYFVSKFGKAAAVA
                     AHLIPEASKLSQGVPWHRVSPLSDNDALAAVFALPASKSALGEEVCVLTATRFGMIKK
                     SLVTELPGPSSQTFVLVKVNEGDKLNSVGITDGKKDILLVTASGMAIRFKEEDVRPMG
                     LVAAGVNGMKLEDKDEVVGMEILPADGDIFLLTNEGKAKRVDQKDFPVQGRYGKGVVA
                     WDMPGKSTLAAVASGKPNHVATVHMSKGAPKSTRLDAAGLRKRAAGKGDVVIEVKPGE
                     SITSIAVGWALESYVKVAKAEEPKNKKEGKPKAAKAPAKKAAKPVAKAKAKPAKKAPA
                     K"
     gene            2726..3886
                     /locus_tag="PLG52_04410"
     CDS             2726..3886
                     /locus_tag="PLG52_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNB40720.1"
                     /translation="MTFITDSQTAPAPYKNDPRIEKDENGTWHVRGYTEARDLLKEDL
                     IQDGFNAEELRKAGFDGVLYMSGDPHRQMRAAIAKYFSPTTVQNTHMTVIEKTADDLL
                     SRLQARKRLNLKELTLEMATVIATAVVGLDPTPGLIQRLNSMLHAPKMSENKLIASLQ
                     HYEGHYRRFAFFFADVLPAIRRKRKNPSNDVISYMISKNKKPLEIWVECIVYGAAGMA
                     TTQEFILVVLLHCMKDAKLREIMTSGDATARYNALNEILRLEPVIGKIYRKPSVPVHV
                     SSEGKEYTIPAGERIAFHVYDVNADAKAVGETPEKLNPHRELEKGVYRSMIGFGSGPH
                     RCAGEHLALAETDVFIQKLLKLPGIRIESGPNIIRNDTVEGYEVNDLIVAVD"
     gene            3953..4381
                     /locus_tag="PLG52_04415"
     CDS             3953..4381
                     /locus_tag="PLG52_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40721.1"
                     /translation="MKLNTKKEVWIARVIAWVLLCFISSGILFVTTFMVGPTLFVVPN
                     LTEKLAVAIYCPGAESTTLETGASTPTTSDPNGTTGHTVEITCTFPDGSTDIIDNGEY
                     AVASIGGMFGIGALIGLLISLPLFVVPFFLIRKRKEPSAL"
     gene            complement(4426..5361)
                     /locus_tag="PLG52_04420"
     CDS             complement(4426..5361)
                     /locus_tag="PLG52_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNB40722.1"
                     /translation="MSDLKRLMPFMVSILVVLACAIPSIPIQNVDTINTNVANTVIAG
                     LTQSVTATFPASSTPSETLAYTSTPTLTYLTARVPSQTPTFTPLPGTDTAIPLLTDTA
                     EPSLVKISVTRPTHCRSGPGKSYEIVGSLLVGMKADVIGRDPTSEYWYIPNPYVFTDY
                     CWVWGEYAEFEGNQLSVPVVMPPPTPTSTATTIPTVDFKLLGSGHHSCDKTHWMNITI
                     LNESNFTFSSINIVMQDKVKNISRSVTYNNFPIQTGCEGLTWVETVPADASATVLGPK
                     FDYNIRGNKLHVTVTVCSEKDMKGACSTRETNLVP"
     gene            complement(5400..>5704)
                     /locus_tag="PLG52_04425"
     CDS             complement(5400..>5704)
                     /locus_tag="PLG52_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40723.1"
                     /translation="VESAQWSFEALVFSTNFHTGFDVEEFQTILPKNYIHRMVKGELI
                     FEFFEAWKRSAAETSHTKIFGLKQWFIASAEALAQKGFQVDRHKKWLNKGYLIWYL"
BASE COUNT         1454 a         1513 c         1276 g         1461 t
ORIGIN      
        1 cctttgcagg agctttcttt gccggttttg cttttgcctt tgcaacaggt ttagccgctt
       61 ttttcgcagg tgcctttgcc gctttcggct ttccttcttt cttgttcttt ggctcttctg
      121 cttttgccac ctttacataa ctctccaaag cccatcccac tgcaatggag gtaatgctct
      181 cacccggctt gacttcgatc actacatcgc ccttgccagc ggcgcgttta cgcagaccag
      241 ccgcatccaa gcgtgtggat ttcggcgcgc ctttgctcat gtgaaccgta gcaacatggt
      301 tcggcttccc agaagccact gccgccaagg tcgatttacc cggcatatcc caagccacta
      361 cgcccttgcc ataacgtccc tgcacgggga agtctttctg atccacacgt ttggctttgc
      421 cttcgttggt caacaagaag atatcgccat cagcaggcaa aatttccata ccgaccactt
      481 catccttatc ttcgagcttc ataccgttca cgcctgccgc cacaagtccc atcgggcgca
      541 catcctcttc cttgaagcgg atcgccatgc cgctggcagt gacgagcaag atatctttct
      601 ttccgtccgt gataccaaca gaattgagct tatcgccttc gttgaccttc accaacacaa
      661 aggtctgtga cgatggaccc ggcaattccg tgaccagact cttcttgatc atgccgaagc
      721 gtgtagcggt caacacgcag acttcctcac ccaacgcaga tttgctcgcg ggcaaggcaa
      781 agaccgccgc cagcgcatca ttgtcactca atggtgaaac acggtgccat ggcacgccct
      841 gactcaactt actcgcttcg gggatcaagt gcgcagcaac tgccgcggct ttcccgaatt
      901 tgcttacaaa atacacagta tcggtagtcg atgccttgac caggaatctc ggcgcatcat
      961 taccggagtg cttaggctgt ttatcgtctg cagtgcgtga gaccaaacca tcatcggtca
     1021 cacccaccca cacagtttgt tgtggcagca aatcattcgt agtgagcgct ttggcagtgc
     1081 cgccttcttt gacacttaca acctgcgtac ggcgacggtc accgtatgcg gcttttactt
     1141 tctgaagttc atcagccacc acaccgcgca tcagttttgg ggtcttcaac agcgtggtca
     1201 attccttgat gatggcagtc acttctttat attcagtttc gattttcttg cgttcgagtg
     1261 cggcaagacg gcgcaactgc atatcaagaa tcgcattagc ttgaatctct gtcagcttgt
     1321 aacgcttcat cagtttagca cgcgctgttt ccacgtctgg tgccgagcgg atgagttcaa
     1381 tgatctcatc caaatttttc aaggcgacga gatatccttc gaggatgtgt gcacgcgcct
     1441 tggctttttc cagatcaaat tccgcgcgcc tcttgatgac cgtgagcctg tgctccaaat
     1501 agacgcgcag cgcctgtttg agagttaagg tgcgcggttc accgtccacc aatgccagca
     1561 agttgatgct gaaggtggtc tgcatcggag tgcgcttgta gaggtcgcgc aacacaaagt
     1621 tcgggtctgc gttcttggtt agttcgagga caatgcgcat accttgacga tccgactcat
     1681 cgcgcaagtc cgatagccct tcgagatgtc catcacgtgc caactcggcg atgcgttcga
     1741 tcaaactcgc cttattgacc atgtatggca gttcagagac gatgatacgg ctcttgccgc
     1801 gttccatctc ttcgatgtgt gccttggctt gcaccgtaat tcgtccacgt ccagaaccgt
     1861 aggcagactc aatgccttcg tcgccttttt cttcaatgat gagaccgccg gttgggaagt
     1921 ctggtccctt tacgaacttc atcaactgct caacatcaat gtcgtcgagc ttctcccagc
     1981 gttcgagcat gaatacgagg gcgtccacaa cttcacttaa gttatgaggc gggatggatg
     2041 tcgccatacc gacggcgata cccgtcgctc cattcacgag cagattcgga atcgcagctg
     2101 gaagcacact cggttcttca agtgtgtcat cgaagttggc agtgaaatcg actgtgtttt
     2161 tgttgagatc attcagaata tcaagcgcag cggatgtaag tctcgcttcg gtataacgca
     2221 tcgcagcggg tgggtcacca tccaccgaac cgaagtttcc ctgaccatct accaacatgg
     2281 tgcgcatcga gaaatcctgc gccaagcgag ccatcgcttc atacacagac gagtcaccat
     2341 gtgggtgata tttacccaac acttcaccca caattctcgc agacttttta tacggcgagt
     2401 cactgcgaat gcccatgtcg tacatggcat ataaaatacg acgctgcacc ggcttcatgc
     2461 catcgcgggc atcgggcaac gcgcgagaca caatgacaga catggcgtaa tcgagatacg
     2521 cctgctgcat ttcgctatcg atatctattt ttcgtacgat accaagttcc atgaaagttc
     2581 ctttaaccac ataggctcaa aaattaaaaa catcctaccc attatggtga attaatgttc
     2641 tattgcgggg cttatcttac gggcaaacaa acgatgcgtc aagtgtactg tataatgtgc
     2701 atatcaactt tagagagagg catacatgac ctttattact gattcacaga ccgcccctgc
     2761 tccatacaaa aatgatccac gcattgaaaa agacgaaaac ggaacctggc atgtgcgcgg
     2821 atacaccgaa gcccgtgacc ttctcaaaga agatctgatc caagatggct tcaacgccga
     2881 agagttacgc aaagccgggt ttgatggcgt gctctacatg agcggcgacc cgcaccgcca
     2941 aatgcgtgct gccattgcca aatatttcag ccccaccact gttcaaaata cacacatgac
     3001 cgtcatcgaa aaaacagccg atgacttgct ctcacgttta caggcacgca aacgtctcaa
     3061 cctcaaagag ttgactcttg aaatggcgac cgtgatcgcc accgccgtag ttgggcttga
     3121 tcccacaccc ggcttgattc aacgcctcaa ctcaatgttg catgccccaa agatgtctga
     3181 gaacaaactc atcgccagct tacaacatta tgaaggtcat tatcgccgct tcgccttctt
     3241 ctttgcagac gtcctgcctg ccattcgccg caaacgcaag aaccccagca atgacgtcat
     3301 ctcctatatg atctccaaga acaaaaagcc gctagagatc tgggtggagt gtatcgtcta
     3361 cggtgcagca ggcatggcaa ccacacaaga atttattttg gtggtcttgt tgcactgcat
     3421 gaaagatgcc aagctacgcg agatcatgac ctcgggcgac gccaccgcac gctacaatgc
     3481 cttgaatgaa atcctgcgtc ttgaacccgt catcggcaag atataccgca aaccttctgt
     3541 gccagtccat gtgagcagcg aaggcaagga atacaccatt cctgctggag agcgcattgc
     3601 ttttcacgtc tatgatgtca atgccgatgc caaagccgta ggtgaaaccc cagagaaatt
     3661 aaatccacac cgtgaattgg aaaaaggggt ctatcgctcc atgatcggct tcggttctgg
     3721 tccgcaccgt tgtgcgggtg aacatctcgc cttagccgaa accgatgtct tcatccaaaa
     3781 gttgctcaaa cttccgggca tccgcatcga aagcggaccg aacatcatcc gcaacgacac
     3841 agtagaagga tacgaagtca acgacttgat cgtagcagtc gactagaaaa aagacagcct
     3901 ttgaaagagg ctgtcttttt atataaatga tgatttccat gagaggtgaa acatgaaact
     3961 taacacaaaa aaagaggtgt ggattgcccg tgtgatcgct tgggtcttgt tgtgttttat
     4021 ttccagtggg attttgttcg ttacaacttt catggtaggt cccactttat tcgtcgttcc
     4081 aaacctcact gagaaacttg ccgttgccat atattgtccc ggtgcagaaa gcaccacgct
     4141 tgaaacaggt gcatctacgc cgaccacttc agaccccaac gggaccacag gtcacaccgt
     4201 ggaaatcacc tgcaccttcc cagacggctc taccgatatt attgacaacg gcgaatatgc
     4261 tgttgcctcc atcgggggca tgttcggtat tggggcattg atcggactgt taatttcatt
     4321 acctttgttt gtggttccgt ttttcttaat tcgcaaaaga aaagagccat cagctttata
     4381 gcatctggct ctaaacacat aaggcacttc atacttcttt gttaactaag gcaccaaatt
     4441 ggtctcacgg gtactacatg cacctttcat atccttctct gaacagacag taactgtcac
     4501 atgcagttta ttaccacgga tgttgtaatc aaatttagga ccaagcacag ttgccgaagc
     4561 atctgcagga accgtctcca cccaagtaag cccttcacaa cctgtctgaa tagggaagtt
     4621 gttgtacgtc acagagcgag aaatattctt gaccttatct tgcatcacaa tattaatgga
     4681 gctaaacgta aaattactct cattaaggat cgtaatattc atccaatgtg ttttatcaca
     4741 agagtgatgc ccgctgccga gtaacttaaa gtcaacagtt ggaatagtcg tcgccgtact
     4801 agtgggagta ggcggcggca taacaacagg gacagataat tgattccctt caaattcagc
     4861 atactccccc catacccaac aataatccgt gaatacatag ggattaggaa tataccaata
     4921 ttcagaagtc gggtctcgcc caattacatc tgctttcatt cctaccaaaa gtgagccaac
     4981 aatctcatat gattttcccg gaccggaccg acagtgagtt gggcgagtaa cgctgatctt
     5041 taccagactc ggttctgctg tatcggttaa taatgggatt gccgtatccg taccaggaag
     5101 tggagtaaag gtcggtgttt gagaaggcac acgtgctgta agataagtca acgttggagt
     5161 ggaagtgtat gccagggttt cagaaggtgt actactggct gggaatgtag ccgttacact
     5221 ttgcgtcaac ccggcaatca cggtgttagc gacattggta tttatggtat caacgttttg
     5281 tatgggaata ctaggaatgg cacaagccaa aacaactaga attgaaacca taaatggcat
     5341 aagtcttttt aaatcagaca tagatgtctc ctattacatt cccaattatg acacgaaaat
     5401 caaagatacc agatcaagta ccctttgttc agccattttt tgtgacgatc cacttgaaaa
     5461 cctttttgcg ccaacgcctc agcagaggca ataaaccatt gctttagccc aaaaatcttg
     5521 gtgtgtgatg tctctgcggc tgagcgtttc catgcctcaa aaaattcgaa gatcaactca
     5581 ccttttacca tacgatggat atagttcttg ggaagaatcg tctgaaattc ttcgacatca
     5641 aaaccggtat ggaaattagt ggagaagacc aacgcctcaa acgaccattg agcggactcc
     5701 accc
//
LOCUS       DAOQNJ010000102         5687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8278_length_5687_cov_8.039595, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000102 DAOQNJ010000000
VERSION     DAOQNJ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8278_length_5687_cov_8.039595"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /gene="bcp"
                     /locus_tag="PLG52_04430"
     CDS             complement(<1..442)
                     /gene="bcp"
                     /locus_tag="PLG52_04430"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNB40724.1"
                     /translation="MPVTSGIPAPTFELLDDTNTLRKLSDYRGKNVVLYFYPKDDTPG
                     CTKEACNFRDDYSAYERAGVVILGISPDSVESHVKFKKKFQLQFPLLADDGHKVCDLY
                     QVWGPKKFMGKAYEGVLRTTFLIDADGNIKKVYEDVRPAEHSQEL"
     gene            665..749
                     /locus_tag="PLG52_04435"
     tRNA            665..749
                     /locus_tag="PLG52_04435"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:699..701,aa:Leu,seq:tag)
     gene            788..2173
                     /gene="tig"
                     /locus_tag="PLG52_04440"
     CDS             788..2173
                     /gene="tig"
                     /locus_tag="PLG52_04440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNB40725.1"
                     /translation="MNIEKQFQDNHSVKLVVEVDQEKMGEYKKRAASKISQRGNIAGF
                     RPGKAPYHIVARTYGEAAITEQAIDLFIDAEYSNILTEAGVNPGASGSLENIESMDPP
                     KFLFSVPLAPEVDLGDYTSVRLPYEWKAPEQKDIDAAIEDLRQMYASTETVERAIESG
                     DYVLLDVTSETTELNRTGFATFVRSEDRDTEWPYNGFAKELIGLKAGESKTIKHSFPK
                     DWEVEDLQGKDVEITATIKTVRGVTLPEINDEFAKTTGAGETVDALMEAIKKDVETRS
                     QNEYDDKYFVDLIEKVKEGATLKYHEHSLEHEGEHVLQDLSQRLAQQGMDLETYFKMR
                     NTTREQFIETDVTPVAKKRLERGLILDEIVRKEQIQIDDDALNAEYMNAINSLAMQGM
                     DFNKIRGGKKGQQELSQAIAMDAASRVMTRKALDMLKSIATGSYKPFEEREAEAKKAA
                     EAETSEEKAAE"
     gene            2348..2959
                     /locus_tag="PLG52_04445"
     CDS             2348..2959
                     /locus_tag="PLG52_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNB40726.1"
                     /translation="MIPDFKNVVPMVVENTGRGERAYDIYSLLLRNNIIFLGTPIDDQ
                     VANVIVAQLLFLNHEDPEKEIRMYINSPGGVIYAGLAIYDTMQIISNPISTVAVGVTA
                     SFGTVLLAAGTKGRRYALPHATVHMHQPLGGAQGQATDIEIQAKQILRLKSLLNGIMA
                     KNTGQPLEVIERDLDRDYYLDAQQAVDYGLVDQIIETPEKAFK"
     gene            3093..3872
                     /locus_tag="PLG52_04450"
     CDS             3093..3872
                     /locus_tag="PLG52_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNB40727.1"
                     /translation="MIPSNIKALILDMDGVIWKGDAPIGDLPATFKRIRERGLKFVFA
                     TNNGTKTPEDYQEKLAELGVDVDPSQVVTSALGVAFMLAQRHPRGTKVFMIGENGIRV
                     ALEEKGFEVVGVEDAPQAEAVVMGIDRGISFQKIAEATLLVRAGRPFYTTNTDRTFPT
                     PRGEIPGSGSWLSVVTSATGVEPMVAGKPFPFLMELSLERLGTSKEETLVVGDRLETD
                     IAAGQAVGCPTAFVLSGVSKLEEAKEWNPSIIADSLAKLVE"
     gene            complement(3922..4953)
                     /locus_tag="PLG52_04455"
     CDS             complement(3922..4953)
                     /locus_tag="PLG52_04455"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB40728.1"
                     /translation="MLKPMKPKQTFPNNTILWILVGVLVIGGIIAIVSAFSGGGKNAE
                     DEVNAVYTNAAATLAIQQMTLQAASPSVTPTVATPTGTVTFTPQPLATLPTSQGISTS
                     SSSSSGGSGAVGCNNSVFVSDVTVPDDTVMTPGQSFTKTWKLQNTGSCPWTTSFKVSF
                     VSGNAMSGTTTALTTTVQAGQSGDISVAMKAPATTGDAIGYWILTNDSGQNFGSSFYV
                     KIKVGGTSTTGTATTTSTATNTSAPSTPVAVGNVSVTLQCSSPDGGLNFDYSGTTISW
                     TDQSNNESGFYIYINNQKVSPDASANATTYTVAANTIFPAGSQIPFGVAAFNGVGAAN
                     IVSAVGACQ"
     gene            5158..>5687
                     /locus_tag="PLG52_04460"
     CDS             5158..>5687
                     /locus_tag="PLG52_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C2)-methyltransferase"
                     /protein_id="HNB40729.1"
                     /translation="MLIYDLNLPALESVLKEWNEPAYRAKQIWQGLYQHLYSSPEQFS
                     NLPNSLRSKMAEHFTFNPFTIKTYLDSSDKSTRKTLFQLSDGNLIESVLMRYGDPADE
                     HSASLEKGTKQRRTLCISTQAGCAMGCVFCATGQLGFKRNLSSGEIVAQVMYYAQMLK
                     GQNEVVSNVVFMGMGEP"
BASE COUNT         1582 a         1362 c         1373 g         1370 t
ORIGIN      
        1 aaagttcctg gctgtgttca gctgggcgca catcttcata tactttcttg atgtttccgt
       61 cagcgtcgat caaaaacgtt gtacgaagaa caccttcata tgctttaccc ataaactttt
      121 taggtcccca cacctgataa aggtcgcaaa ctttgtgtcc atcatcagca agcaagggaa
      181 attgaagctg gaatttcttt ttaaacttca catgagattc aacggagtct gggctaatgc
      241 ctagaatcac cacccctgcc ctttcgtagg cagagtaatc atcgcgaaag ttgcacgctt
      301 cttttgtgca accgggtgta tcatctttcg gataaaaata taagacgaca ttctttccac
      361 gataatcgga aagtttgcgt aaggtattgg tatcgtcaag caattcaaat gtaggggcag
      421 gaatgccaga agtaacgggc ataaaaaatg tactctctct ccaaagaagt atgcgcgtta
      481 ttataccttt attcatttta agggtaggaa aactttcaca attatttggt gggcagcact
      541 ccaattttcg gtagaaaacc ggctttaagg catggatcaa tctccttata aaaaacctca
      601 gaaaaaaatg gcgattcggg cattccctac ggtataattg cgcccgcctg taagcacaca
      661 aaccggggat gtgctggaac tggcagacag gcatgactta ggatcatgtg ccgcaaggcg
      721 tgagggttcg accccctcca tccccaccaa aataatattc agaccttatt ggtcttcgga
      781 ggaaccattg aacatcgaaa agcaatttca agataatcac tcagttaagc tggtcgtaga
      841 agtagaccag gagaaaatgg gcgaatataa aaagcgcgca gcgagcaaaa tttctcaacg
      901 cggtaatatc gccggctttc gccccggcaa agctccttat cacatcgtag cccgcaccta
      961 tggcgaagcg gccatcaccg aacaagccat tgacttgttc atcgacgcgg aatattccaa
     1021 cattttgacc gaagctggtg ttaaccccgg cgcttctggc agcttggaaa acatcgaaag
     1081 catggatcca cccaagttcc tctttagtgt gccactcgca cctgaagtgg acctcggtga
     1141 ttacacctcg gtacgcctcc cctacgaatg gaaagcccct gaacagaagg atattgacgc
     1201 cgcgattgaa gacctgcgcc aaatgtacgc ctccaccgag accgtggaac gtgccatcga
     1261 atctggagat tatgtgctcc tcgatgttac ctctgagaca actgaactca accgcactgg
     1321 ctttgctacg tttgtccgca gcgaagaccg tgacactgaa tggccataca acggtttcgc
     1381 caaggaactt attggtttga aggctggcga aagcaagacc atcaagcaca gcttccccaa
     1441 agattgggaa gtggaagacc tgcaaggcaa ggatgtggaa atcaccgcaa ccatcaaaac
     1501 cgtgcgcggc gtgacccttc ctgaaatcaa tgatgaattc gccaagacga ccggcgctgg
     1561 cgaaactgtc gatgcattaa tggaagcgat caagaaagat gttgaaaccc gctctcagaa
     1621 cgaatatgac gataagtact tcgtggattt gatcgaaaag gtcaaggaag gcgcgaccct
     1681 caagtatcac gaacactcgc ttgagcacga aggcgaacat gttctgcaag atctctctca
     1741 gcgacttgcc cagcaaggta tggaccttga aacctatttc aagatgcgca acaccacccg
     1801 cgaacagttc attgaaaccg atgtgacccc ggtggcaaag aaacgcctcg aacgcggttt
     1861 gatccttgat gagatcgtgc gcaaggaaca gattcaaatt gacgacgatg ccctgaatgc
     1921 agaatatatg aatgctatca acagcctcgc tatgcaaggc atggacttta acaagatccg
     1981 cggcggaaag aaaggtcaac aagagttgag ccaagccatt gccatggatg cggcaagccg
     2041 tgtgatgacc cgcaaggcgt tggatatgtt gaagagcatc gcgaccggta gctacaagcc
     2101 gtttgaagaa cgcgaagccg aagccaagaa agccgctgaa gctgaaacta gcgaagaaaa
     2161 agccgcagag taagaaaagg cgtttcgaag gctggcaggc ttaggtccaa gaaatgaagc
     2221 gtgcgccgcg tcccatcggg tagaatcctc tcatcgaacc gggcgcggcg catttatatc
     2281 gcctatcaaa ctctaatata aatccaatag tccctcggta acattggtcg aataaaggag
     2341 accgaacatg atcccagatt tcaaaaatgt agttcccatg gtggttgaaa atacaggtcg
     2401 cggcgaacgc gcttatgaca tttactcgct gctcctgcgt aacaacatca tcttcctcgg
     2461 cacgcccatc gacgaccagg tcgcgaatgt gatcgttgcc cagcttttgt tccttaatca
     2521 cgaagaccct gaaaaagaaa tccgtatgta catcaactcg cctggcggtg tgatttatgc
     2581 ggggctcgca atctatgaca cgatgcagat tatctcgaac cccatcagca ccgttgccgt
     2641 gggtgtgacc gcctctttcg ggaccgtgtt gctggctgct ggaaccaagg gtcgacgcta
     2701 cgccctgccc catgccaccg tccacatgca ccagcctctc ggcggcgcac aaggtcaggc
     2761 gacagatatc gaaattcagg cgaagcagat tttgcgcctc aagagcctgc tcaatggcat
     2821 tatggcgaag aacaccggtc agccgctcga agtgatcgaa cgcgacctcg accgtgatta
     2881 ctatctcgat gctcaacagg ctgtggatta cggcttggtg gatcagatca tcgaaacccc
     2941 tgaaaaagca ttcaaatagt tattaatacg gtagctatga gacctcgaag agaaatcttc
     3001 ggggtctttc ttttataggt ataatgcccg ctgcgttgtt cgtgatacgt aaaccgtaca
     3061 tcgaaaatct aaaatcgcaa actgtaaatt gtatgattcc ttcaaatatc aaagcactta
     3121 ttctcgacat ggacggtgtg atctggaaag gtgacgcccc catcggtgac ttgcccgcta
     3181 cgttcaaacg aatccgtgag cgcgggttga agttcgtatt cgctacaaat aatggaacca
     3241 agacgcctga agattatcaa gaaaagcttg ccgaactcgg cgtggacgtg gacccttcac
     3301 aagtcgtcac atcggcatta ggcgtggcat tcatgttggc acagcggcat ccgcgtggaa
     3361 cgaaggtttt tatgatcggt gaaaatggca tccgtgtggc gttggaagaa aaggggtttg
     3421 aagtcgttgg cgtggaggat gccccacaag cggaagccgt tgtaatgggc attgaccgtg
     3481 gcatcagctt ccaaaaaatt gccgaagcga ctttgctggt gcgggcgggt cgtccgtttt
     3541 acacgaccaa tacagaccga accttcccca ctccgcgcgg agaaatcccc ggttctgggt
     3601 cttggctctc tgtggtcaca tcggctacgg gtgtggagcc gatggtcgct ggcaaacctt
     3661 tccccttctt aatggagctt tcgctcgaac ggttgggaac atcaaaagaa gaaaccttgg
     3721 tcgttggcga ccgcctcgaa acggatattg cggcaggtca ggctgtgggc tgcccaaccg
     3781 cattcgtatt gagcggggtt tccaaactcg aagaagcgaa agagtggaat ccgagcatca
     3841 ttgctgatag tttagcaaag ttagtggaat aaaaaaaacg cctgtgtttt caacacaggc
     3901 gtttttttta tagaaacact tctattgaca ggcgccgaca gcacttacta tgtttgcagc
     3961 accaactcca ttaaaggctg ctacaccaaa aggaatttgg ctccctgcag ggaatatagt
     4021 gtttgctgca acagtataag ttgtagcatt agcagaagca tccggagaga ctttttgatt
     4081 gtttatatat atataaaaac cgctctcatt gtttgactga tcagtccaag aaatagttgt
     4141 tcctgaataa tcaaaattca atccgccgtc aggtgaagaa cactggagag tgacactaac
     4201 attacctact gcaacaggag tagatggagc gctcgtgtta gtagcagtcg atgtagtagt
     4261 ggcagtacct gttgtagaag tgccacctac tttgatctta acatagaaag aagagccaaa
     4321 attctgcccg ctatcgttgg tcaaaatcca atagccgatg gcgtcacctg tagttgcagg
     4381 agctttcata gctactgaaa tatcaccaga ttgtccagct tgaacggtag tagtaagagc
     4441 agtagtagtc ccgctcatcg cattgccaga aacaaagcta actttgaacg acgttgtcca
     4501 cgggcaggag ccggtgtttt gcaatttcca agtcttggtg aaagactgtc cgggggtcat
     4561 taccgtatca tccggcacgg tcacatctga gacaaagacc gagttgttac atcccaccgc
     4621 gccagagccg ccagacgaag aggaagagct ggtcgaaatc ccctgcgatg ttgggagggt
     4681 ggcaaggggc tgtggagtga aggtcactgt ccctgttggg gttgcaacag ttggggttac
     4741 agacggggaa gccgcctgca aggtcatctg ctgaatagca agggtcgctg cagcattagt
     4801 gtataccgcg ttaacttcat cctctgcatt ctttcctccc ccactaaatg cagacacaat
     4861 ggcaataatg ccgccaataa caaggactcc caccagaatc cacagaatag tgttatttgg
     4921 aaaagtttgc tttggtttca ttggtttcaa cataaatttc tccttcgttt tcaagcaagg
     4981 cttagaatta tatttggaaa aaattaagaa aacataggta tttccctacg aatttcctac
     5041 ttttcttgta cgatacgggc agaattttat agatttatga cggtctccat gagagataag
     5101 ttccattctt acgaaaaatg ggatttccat ttgctggtgt aaaataggaa accatttatg
     5161 ttaatttacg atcttaacct tcccgctctt gaatctgtct taaaagaatg gaatgaacct
     5221 gcataccgcg ccaagcaaat ctggcaggga ctgtatcaac atctttattc ttcaccagaa
     5281 caattcagca atctgccgaa ttccctgcgc agcaaaatgg ctgagcattt tacctttaat
     5341 ccattcacaa tcaaaaccta tttagactca tcggataaat ccactcgtaa aacattattt
     5401 cagctttcgg atggaaattt aatcgagtct gtcttgatgc gttatggtga ccctgcggat
     5461 gaacattctg cttctttaga aaagggtacc aaacaaaggc gcacgctttg catctccact
     5521 caggctggct gtgcaatggg atgtgtcttc tgcgccacgg gtcagctcgg gtttaagcgc
     5581 aatctttcca gcggtgagat cgtggcacag gtcatgtatt acgcgcaaat gctcaaagga
     5641 caaaacgaag ttgtaagcaa cgtcgttttt atgggcatgg gcgagcc
//
LOCUS       DAOQNJ010000103         5683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8290_length_5683_cov_11.391791, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000103 DAOQNJ010000000
VERSION     DAOQNJ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8290_length_5683_cov_11.391791"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLG52_04465"
     CDS             <1..192
                     /locus_tag="PLG52_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40730.1"
                     /translation="VQVGKGLNPQEWIQLGEDNYTPVEGGILAEWDTTGLSGLYAVQL
                     IVVRQDQKVDTAVIQVTVK"
     gene            complement(233..2113)
                     /locus_tag="PLG52_04470"
     CDS             complement(233..2113)
                     /locus_tag="PLG52_04470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNB40731.1"
                     /translation="MAYKLSKWDLSQLYSGYDSPELENAFDMIEEQVASFEGVRSKLT
                     PDISAEQFMQVMKADELSTRVANKLYSFAGLSFTADTQDQKAQALQARVQQFLAENQN
                     RILFFSLWWKELDDANAKRLMDASGDYRYYLESMRNFKPHTLSEAEEKIINLKNVNGV
                     NALGNLYDSITNRYVFKMKVGGKEKEMTAAELFSYRYSTDPAVRAASYQAQFKVYAED
                     GPILGQIYQTILRDWHSENIGLRKFKNSIAPRNLSNDVPDEAVDALLNVARKNTGIFQ
                     RYFTMKAKHLGMKKIRRYDIYAPLAASKKTYDWDTAVHMVLDAFNNFSPKVAELAQRV
                     FDQNRIDSEIRKGKRGGAFCWGFLPEETPYVLVNYQGNSREVATLAHELGHAIHAMLA
                     EQHSVYTFHSSLPLAETASTFAEMVLIDKLLTEETDESVRRDILFKQMDDAYATVLRQ
                     SYFALFEKTAHEMTQKNASIDDLSAAYLENLKEQFGDSLELSDEFKWEWVGIPHIYQT
                     PFYVYAYAFGQLLVYALYQQFKQEGESFKPKYLKILSAGGSEAPERILSEAGVNIRDP
                     KFWQGGFDFLERLVSELEKLPVEKAKKPAAKKVAKKAVKKVAAKKPAKKAPVKKVAKK
                     KK"
     gene            complement(2210..3268)
                     /gene="ychF"
                     /locus_tag="PLG52_04475"
     CDS             complement(2210..3268)
                     /gene="ychF"
                     /locus_tag="PLG52_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNB40732.1"
                     /translation="MKLGIIGLPQSGKTTIFNALTRGNAPTNASAGRIEVHQSVVDVP
                     DPRVDLLSKMFNPRKTIYTKITYADIAGLDSGSAKSGISGQLLNHLNQMDALILVVRA
                     FENDNVAHPNGSINPLRDVDTMTSELLLNDLIAVERKLERLTDERKKGGTDKTLNARQ
                     TELFEKMHKALSDNKPLRDLEYTPEEEKELSSFGLLTRKRLLTVFNLGEGQSAPDAKL
                     DHPSVALMGKLEMEIAQLSAEDAAVFMDEYGIKELSLNRMINLSYELLQELTFFTVGE
                     DEVRAWVTHRGATAQEAAGEIHTDLSRGFVRAEVVAYEDLISLGSMNEAKAKGKFRLE
                     GKEYIVKEGDIMHVRSSL"
     gene            3335..3417
                     /locus_tag="PLG52_04480"
     tRNA            3335..3417
                     /locus_tag="PLG52_04480"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3369..3371,aa:Leu,seq:caa)
     gene            3779..5029
                     /locus_tag="PLG52_04485"
     CDS             3779..5029
                     /locus_tag="PLG52_04485"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNB40733.1"
                     /translation="MPSYNEVLAVILGGGRGARLYPLTQMRSKPAVPIAGKYRLIDIP
                     ISNCINSEIFRIAILTQFNSVSLHRHITRTYNFDSFHQGWVQIWAAEQTIESADWYQG
                     TADAVRKQMLEIQATGAKYVLILAGDHLYRMDYKAMAEFHWANNADITVAVQPVAKDE
                     ANRFGVLKRESDGTISDFYEKPKDPDVQKRFVSRDDPERPFLGSMGIYMFNTKVLLDF
                     ISNFPDYDDFGGDIIPQAIKTHKVFGFDFDGYWQDIGTIRSFYETNLMLTKSNTPFDF
                     YDAKLPIYTDTRYLPSSIVEDSQMKDVLIAEGSRVLKSEITHSIIGIRSQIAGGCVIK
                     DSIVMGSDYYEREHRAGTPLGIGPNCHIEGAILDKNVHIGENVTIRPFPRNTDIDSEK
                     WYVRDGIVIIPKDVEIPSGTVIAP"
     gene            5153..5398
                     /locus_tag="PLG52_04490"
     CDS             5153..5398
                     /locus_tag="PLG52_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40734.1"
                     /translation="MKSTREFWQRWAESLRRYQLHDLVASFLEAGSPLALLGAQAIYF
                     GGGLVKNDQLTALAGLLEDENETRAFVAFLSHNGASS"
     gene            5395..>5683
                     /locus_tag="PLG52_04495"
     CDS             5395..>5683
                     /locus_tag="PLG52_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40735.1"
                     /translation="MTALAILGVAAFLLLSLTLWKRRSPSYLREIPAFTKLAHSLGLS
                     IEDGKRLHISLGHGGLFDARGGSAFAGLALLRNIAERTSVSDMPAVASAGDP"
BASE COUNT         1371 a         1364 c         1377 g         1571 t
ORIGIN      
        1 gttcaagtgg ggaaggggct gaatccgcaa gagtggattc aattgggcga agataattac
       61 accccggtag aaggcggcat cttagctgaa tgggatacaa ccggtctaag cggtctgtat
      121 gccgttcagt tgattgttgt ccggcaggat caaaaagtag atacagccgt tattcaagtg
      181 acagtgaaat agttcaaata aaaagacccg gctcattacc gggtcttttt atttacttct
      241 ttttcttcgc aaccttcttt acaggtgctt tcttggcagg ctttttcgct gccactttct
      301 ttacagcttt cttggcgact ttctttgccg caggcttttt ggctttttcc actggcagtt
      361 tttccaactc actcaccaaa cgttcgagga agtcgaaacc accttgccag aatttcgggt
      421 cacgaatgtt gacaccggct tcgctcaaaa tgcgttctgg cgcttctgaa ccgcccgccg
      481 aaaggatctt gaggtacttc ggcttgaagg attcaccttc ctgcttgaat tgctgataca
      541 aggcgtacac cagcaattga ccgaaagcat aggcatagac gtaaaacgga gtctgataga
      601 tgtgcggaat gccgacccat tcccatttga attcgtcgct cagttcgagc gagtcaccaa
      661 attgttcttt gaggttttca aggtacgcag cgctcaagtc atcgattgag gcgttctttt
      721 gagtcatctc atgcgcggtc ttttcaaaca gcgcaaagta cgattgacgc agcacggtcg
      781 cataggcatc atccatttgt ttgaagagaa tgtctcggcg aaccgattca tctgtttctt
      841 ccgtgagcaa tttgtcgatc aagaccatct cagcaaaggt cgaggcggtt tcagccaacg
      901 gtagtgacga atggaaggtg tagacactgt gctgttctgc cagcatggca tgaatcgcat
      961 gacccagttc gtgcgcaaga gtggcaactt cacggctatt gccctgatag tttaccaaca
     1021 catagggagt ctcttcgggt aaaaagcccc aacagaaagc gccgccgcgt ttgcctttgc
     1081 ggatctcgct atcaatgcga ttctggtcaa agacgcgctg ggcgagttct gccactttgg
     1141 gcgagaaatt attgaatgca tccaacacca tgtgaacggc agtgtcccaa tcataagttt
     1201 tcttggaagc agccaacgga gcatagatgt catagcggcg gatcttcttc atgccaagat
     1261 gtttggcttt catggtgaag taccgttgga agatacctgt attcttgcgt gccacattga
     1321 gtaaggcatc caccgcttcg tcagggacgt cgttgcttaa gttgcgcggc gcaatggagt
     1381 tcttaaattt gcgcagccca atattctcac tgtgccaatc gcgcaagatg gtctggtaaa
     1441 tttgcccaag gatgggacca tcctctgcgt ataccttgaa ttgcgcctgg tagcttgcgg
     1501 cgcggacggc cgggtctgtg ctgtagcggt aagagaacag ctcggcagcg gtcatttctt
     1561 tttctttgcc acctactttc atcttgaaca catagcggtt cgtgatcgag tcgtacaggt
     1621 tgcccaacgc attcaccccg ttgacgttct tcaagttgat gatcttttct tctgcttcgc
     1681 tcaaggtatg cggcttgaag ttacgcatgg attcgagata gtagcgataa tcgccagaag
     1741 catccatcaa acgtttggcg ttggcgtcat ccagttcttt ccaccacaag ctgaaaaaca
     1801 aaatgcggtt ctggttctct gctaaaaatt gttgaacacg ggcttgtaaa gcctgtgctt
     1861 tttggtcttg tgtatctgct gtgaaggata aacccgcaaa cgaataaagt ttgtttgcaa
     1921 cgcgggttga aagctcatcg gctttcatca cctgcataaa ttgttcggca gaaatatcgg
     1981 gcgtaagttt gctgcgcacg ccttcgaaag atgccacttg ttcttcgatc atatcgaatg
     2041 cattttcaag ctctgggctg tcgtagccag aataaagttg agaaaggtcc cacttggaaa
     2101 gtttatacgc catgttgtct ccttgtttta ttcgtagagg gacgagttgc cctcgtccct
     2161 acgttttatc acaacttaaa aaactttaac ccattctacg gtgtggattt tacaaactgg
     2221 accgcacgtg catgatatcg ccttctttga cgatatattc tttgccctcc agccggaact
     2281 tgccctttgc cttcgcttca ttcatggacc cgaggctgat caaatcttca taagccacca
     2341 cctcggcgcg aacgaatcca cgcgaaagat cggtgtgaat ttctccggcg gcttcctgtg
     2401 ctgtcgcgcc gcggtgggtg acccaggcgc gcacttcatc ttcacccacg gtgaagaagg
     2461 ttaactcttg caacaactca tacgagaggt tgatcatgcg gttgaggctc aactccttga
     2521 tgccatattc atccatgaac acggctgcat cttccgcaga gagctgcgcg atctccattt
     2581 caagtttgcc catcaacgcc accgaaggat gatctaattt cgcatccggc gcagactgtc
     2641 cctcgccaag gttgaacacg gttaacaaac gtttgcgggt gagcaagccg aagctggaaa
     2701 gttctttttc ttcttcaggg gtgtattcca aatcgcgcaa tggtttgtta tcagaaagtg
     2761 ccttgtgcat tttctcaaat aactcggttt ggcgggcatt gagagttttg tctgtgccgc
     2821 cttttttgcg ttcatcggtg aggcgttcta atttccgttc aacggcaatg aggtcgttca
     2881 acaacaactc actggtcatc gtatccacat cgcgcaaggg attgatgctt ccgttcgggt
     2941 gcgcgacatt atcattctca aaagcacgaa cgaccagaat aagcgcgtcc atctgattga
     3001 ggtgattcaa caactgaccc gaaataccgc ttttggcaga gccgctatcc aaaccagcaa
     3061 tatcggcata ggtgatcttg gtgtagattg tcttgcgtgg gttgaacatt ttggaaagaa
     3121 ggtccacacg cgggtcgggt acatccacta cagactgatg gacttcgatg cgtccagcag
     3181 atgcgttggt gggggcgttg ccacgggtga gggcgttgaa aatcgtcgtt tttccagatt
     3241 gggggagtcc aataattcct agcttcatct tgaccttatc aatgggagtg gtatacttgc
     3301 gttcgcctcg ctaatgcgag accacaacaa ttaagccgaa gtggcggaat aggcagacgc
     3361 gcacgactca aaatcgtgtt ccctccggga atgagggttc gattccctcc ttcggcacac
     3421 ggattatacc ctacgggtac ttgaccctaa gagcaaataa tccacctctg gcagcgtcct
     3481 ccgcaatgga ggacgtttga tttttatatg taccctactc tgtaaggttg accgggtcat
     3541 ttggggattg tagaatgaaa atagaaacaa gtttgggttt tatacgtatt tatattaagc
     3601 tgtctagacc cgtaggagga gtccctcatg aaactttctc tctttttcat gttgtttgat
     3661 gttttcgtga ttttggcata cccaattgct tatgtaattc atcgcattcg taaggtgaag
     3721 ggtgtaaaat agggagcatt gctcgacatt cctgaacgaa aattccagga ggctccatat
     3781 gccgtcgtac aacgaagtcc tggctgtgat ccttgggggt ggacgcgggg cacgccttta
     3841 cccgctcaca caaatgagat ccaagcctgc cgtcccgatt gcgggaaaat accgtctcat
     3901 tgacattccc attagtaatt gtattaactc agagatattt cgtattgcga ttctgaccca
     3961 gttcaactcg gtctcattgc atcgtcatat cacccgcacc tataattttg attctttcca
     4021 tcagggttgg gtgcagatct gggcagccga acagacgata gaaagtgccg actggtatca
     4081 aggcacagcc gatgctgttc gcaagcagat gcttgaaatt caagccacgg gtgcaaagta
     4141 tgtactcatc cttgcagggg atcatctgta ccgtatggac tacaaggcaa tggctgagtt
     4201 tcactgggcg aataatgcag atatcaccgt cgccgtgcaa cccgttgcga aggacgaagc
     4261 aaatcgcttc ggcgtcttga agcgtgaatc agacggtacg atctcagatt tttacgaaaa
     4321 gcctaaagat ccagacgtcc aaaaaagatt tgtcagccgt gacgaccctg aacgaccttt
     4381 tctcggttcc atgggtatct atatgttcaa caccaaagta ctgttggact ttatcagcaa
     4441 cttcccagat tacgatgact ttggcggcga tattattccg caagcgatta agacccataa
     4501 agtctttggc tttgattttg acggctactg gcaagacatt ggaacgatcc gctcgttcta
     4561 tgaaaccaat ttgatgctga cgaaatcgaa tactccgttt gatttctacg atgcaaaact
     4621 gccgatctat accgatacgc gttatctgcc ttcttccatt gtggaagata gccagatgaa
     4681 agatgtattg atcgctgaag gtagccgggt cttgaaatct gagatcacgc attcgatcat
     4741 tggtattcgc agtcagatcg ctggtggctg tgtgatcaaa gacagcattg tgatgggttc
     4801 tgattactat gaacgtgaac accgtgcagg cacaccctta ggaattggac caaattgcca
     4861 cattgaaggt gccattctcg ataagaatgt acacattggc gagaatgtaa ccattcgccc
     4921 cttcccacgt aataccgata tcgacagcga aaaatggtat gtgcgagatg gtattgtcat
     4981 tattccaaaa gatgtagaga taccatcggg tacggtcatc gctccataaa caatgaaccc
     5041 ctgaagatcg aattcttcag gggttttttc ttgaaatatt cacaaaactg taacatttag
     5101 cgctcttatg cagcaggggc aaccttaaaa ttatgctata ctcgcattaa ctatgaagtc
     5161 aacgcgcgaa ttctggcaac gctgggctga gtccttgcgc cgttatcaac ttcacgatct
     5221 tgtcgcctca tttctagaag caggaagccc gcttgccctg ctcggtgctc aggcgattta
     5281 ttttggtggg ggacttgtca agaacgacca acttacagct cttgccggtt tgcttgaaga
     5341 tgaaaacgag acacgagcct ttgtcgcttt tttgagtcac aatggagcga gttcatgacc
     5401 gcactagcta ttcttggcgt cgcagctttc ttattactta gccttacatt atggaagcga
     5461 cgctctccct catatttacg tgaaattccc gcttttacaa agcttgctca ttctcttggc
     5521 ttaagcatcg aagacggcaa acgtcttcac atttcgcttg ggcatggcgg cctgttcgat
     5581 gcacgtggcg gatctgcctt cgccgggctt gctctactgc ggaatattgc tgagcgcacc
     5641 tctgtgagtg atatgcctgc tgtcgcctcg gcaggggacc cga
//
LOCUS       DAOQNJ010000104         5659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8342_length_5659_cov_5.827445, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000104 DAOQNJ010000000
VERSION     DAOQNJ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8342_length_5659_cov_5.827445"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2217)
                     /locus_tag="PLG52_04500"
     CDS             complement(<1..2217)
                     /locus_tag="PLG52_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HNB40736.1"
                     /translation="MSRAENKTERLLQMEALLLSNPQGLTQAEIARRLGVDRSVVNRN
                     LYDFQKIYPTITHEDGRISLDRSAYLVKVAFTLHESTAVHLAARLLATRMDRQNPHAA
                     SALRKLGIALEKLAPRISTHVKQSAEVMDDATQWQDPRYLDVLEKLTLAWAELRKVKI
                     WHRSDKAQKVLEYFLCPYFIEPYAVGQTTHLIAHDESTGKMRTLKIERIEKVEVTQEF
                     YEIPSDFDPRDLLADAWGIWYTTSDPVEVTLKFHRSVAPRLEETRWHRSEEEIKLEDG
                     SVIWKAKVAEPQEMIPWIRSWGADCEVLEPKTLREALAREAHSLAELYQVMEMTPKYY
                     GHSLKGKDKSEWQLLKTHLSDTAVKAKEFGEDAGIGDLAYTAALLHDIGKYSKAFQAR
                     LDGNGRKVDHATAGAREVLKLFNKDGLEKNLATMLSYCIAGHHTGLPDYGDATDVDGT
                     GTLLSRVGRKKLEDYSAYKTEINSASLELKSRKLKTSNEAFSVAFLTRMIFSALVDAD
                     FQDTETFMQQAEKPRGGHASIEELCNTFNAAIQKFDNPQGEINKKRTETLKACKEGAE
                     LDQGFFTLTIPTGGGKTLASMAFALNHAKRHGLKRIIYVIPFTSIIEQNAGVFKEYLG
                     KDNILEHHSNFDWKKGNKTNSDEADDQTKEALDKLKLASENWDIPIVVTTNVQFFESL
                     YANKSSSCRKLHNIAKSVIIFDEAQMLPREYLDPCMLAIKELVTNYGASAVFCTATQ"
     gene            complement(2289..2507)
                     /locus_tag="PLG52_04505"
     CDS             complement(2289..2507)
                     /locus_tag="PLG52_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40737.1"
                     /translation="MARKIEEKKLEEIFEKVEQNPGKKAGFIARLLGLNRSEVTRSLP
                     ALEDKGLYIYEDDEGGLYPHQRNQPQNS"
     gene            complement(2584..4581)
                     /locus_tag="PLG52_04510"
     CDS             complement(2584..4581)
                     /locus_tag="PLG52_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB40738.1"
                     /translation="MAHILPDTPPQLIPKEVLRVYRALKTLPDSYFIWHHLAPWQVDM
                     PDFLIFNETGQALLVKVSLSTEEKTRTASQMLLLGEEKEPLGVQENKLLHAFVSSLGI
                     PPDQHVETLVIFPNIPDKHVQASRLARGEGEPQWVGKEIVQPESNWDAFLPSSPMDAI
                     WLEKLRQRFTPEVVVPADMTVRLEGTRRREAGLTDYLLDYDQERAVKYELELPPDDQA
                     LPADMRLNIINGVAGSGKTLILLYRLRLLYHLYPDKRFLVLTHNRPISHDMQGRFTRL
                     MGREVNHIEWRTFSAWCHHHWMNKPAWVSPLSLSAREHRLNEIWRSHLRDTKITQKMF
                     QSELDWFKDQLPLRRTEYLTTDRRGRGFGLSEEMRGRMFNAFLAYQTSLKAKGELDWG
                     DVPQRLWKAVTESAHQLPEYDFILIDEAQFFAPLWMHLIQKALNPHGHLFLVADPTQG
                     FLGRGTSWRSLGLDARGHTHTLKKSYRTTREIMQFATLLYRLRLENEKDEDILTPDIL
                     NMPKGAFPELIPLTSPQDEVARIANEVENLVKQGLPRKHILLLHTDGNGVQHLIQALE
                     DRLGKGSAIDPKDTFPGDYIRVTTFNAGAGLESQIVFLVGLKALFEEEQSLRLADDER
                     EELIRDNTRKLYMAATRAGQRLVLSYSGELPETLKKVFLHG"
     gene            complement(4585..>5659)
                     /locus_tag="PLG52_04515"
     CDS             complement(4585..>5659)
                     /locus_tag="PLG52_04515"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB40739.1"
                     /translation="FVTALTHRAIHNALNKINQVDPSLPICKIGEDKSISDLDVPNYI
                     NFGASRFGEMNGGYVIGATPFASQTNRLSGVEFDVVLFDEASQITLPLAIMGMLAGSK
                     YIFIGDENQLPPVTIFSEEEAVQNSIFNYLSGHGNETMLNITYRLNDVLTRWPSRNFY
                     RDELRSSPQAAPRRLDLSPEATPWDMVLDPASPIVFLDLCHRNSTVRSRLEAGVVLEL
                     ILSLLTREVHPREIGVVVPYRAQSRLIRSLLRRTLEDGDVFDQIVVDTVERMQGQERE
                     VILVSFTTASPKFAAQMADFLFSPNRLNVAVTRPRSKLILVGSHHMLAADEYDASKKE
                     TLDLLRNLIQGSTHITVPDGNLT"
BASE COUNT         1386 a         1455 c         1349 g         1469 t
ORIGIN      
        1 ctgggtcgct gtacaaaaga ctgcgcttgc gccataattc gtaaccaatt ccttgatcgc
       61 caacatacag gggtctaaat actctcgggg taacatttgc gcttcgtcaa aaataatgac
      121 gcttttggct atgttgtgca atttacggca ggatgaagat ttatttgcat acaaagactc
      181 gaagaactga acattcgtag tcactacaat gggaatatcc caattctcag aagccagttt
      241 cagtttatct aatgcttctt tggtttggtc atcggcttca tcagagtttg tcttattccc
      301 cttcttccaa tcaaaattag aatgatgctc caaaatattg tctttaccta agtattcctt
      361 gaatacgcct gcattttgtt caatgatgct agtaaatgga atgacgtaga ttatccgctt
      421 tagcccgtgt cgtttcgcat ggttcaaggc aaatgccatc gaagccagag tttttccgcc
      481 cccagtagga atggtcaaag tgaagaaacc ctgatcaagt tctgcaccct ctttacaggc
      541 tttcagggtc tcagtacgct ttttgttgat ttcgccttgc gggttatcaa atttctgaat
      601 ggcagcgttg aatgtattgc aaagctcttc gatgctggcg tgtccaccac gtggtttttc
      661 tgcttgttgc atgaaggttt cggtgtcttg aaaatccgca tccactaagg cagagaaaat
      721 catccgtgtc agaaaggcaa ctgaaaacgc ctcgttcgat gttttgagtt tacggctctt
      781 taattcaaga gatgcagaat ttatttctgt tttataggcg ctgtaatcct ctagtttttt
      841 cctgccgaca cgagacagta aagttcctgt tccatctaca tcagtagcat cgccataatc
      901 aggaagccca gtatgatgac cagcaatgca ataggaaagc atggttgcaa ggtttttttc
      961 aagtccatct ttgttgaata gtttcagcac ttcccttgct cctgctgtcg catggtctac
     1021 ttttctgcca ttgccatcta acctagcttg gaacgccttg gaatatttac caatgtcatg
     1081 aagcaaggca gccgtatatg ccaaatcgcc tatacctgca tcttccccaa attctttagc
     1141 ttttactgct gtgtcagata agtgcgtttt caacaattgc cattcagatt tatctttgcc
     1201 tttgagtgaa tgaccataat actttggcgt catttccatc acctgataca actccgccaa
     1261 agaatgcgcc tcccttgcca gtgcctcacg caaggttttt ggctccagca cttcacagtc
     1321 cgcgccccac gagcgaatcc aaggaatcat ctcctgtggt tcggcaacct ttgccttcca
     1381 aatgaccgaa ccatcctcca acttgatttc ttcttcagag cgatgccagc gggtttcttc
     1441 taaacgtgga gcaacactac gatggaactt caaagtcact tccacagggt cgctggtggt
     1501 gtaccaaatt ccccaggcgt ctgctagcaa atcacgcggg tcgaaatcag atggaatttc
     1561 ataaaattct tgcgtcacct ccaccttttc aatgcgctct atcttcaacg tccgcatctt
     1621 ccctgtagat tcgtcgtgtg cgatcaagtg tgtggtttgt cccaccgcat aaggctcaat
     1681 aaaatatgga caaaggaaat actccagaac tttctgtgcc ttgtcgcttc gatgccagat
     1741 ttttaccttg cgaagttctg cccaggcgag tgtcaatttt tcaaggacat ccaaatagcg
     1801 cggatcctgc cattgcgtcg catcatccat tacctcagca gattgcttta catgggtgct
     1861 gatacggggg gcgagttttt ccaatgcgat gcccaacttc cgcagcgcag aggcagcatg
     1921 gggattttgc ctgtccatgc gggtggcaag cagacgtgca gcaaggtgaa ccgcagtcga
     1981 ttcgtgcaga gtaaatgcca ctttgacgag ataagccgaa cggtcaaggc tgatgcgccc
     2041 atcttcgtgg gtgatagtgg ggtaaatttt ttggaagtcg tataaattgc gattgaccac
     2101 cgaacggtcc acgccaaggc gccgcgcaat ttcagcttgc gtcaagcctt gagggttgga
     2161 cagcaggagt gcttccatct gtaaaagtcg ttcagtcttg ttttcagcac gagacatagc
     2221 aaactccgca acaggtttta tttatgaaca gaatagcatg caaaagtgtt gaattctgca
     2281 acattttgtc atgaattttg cggttgattt ctttggtggg gatacaatcc cccttcatca
     2341 tcttcataaa tataaagtcc tttatcctcc aacgctggta gcgaacgtgt cacctccgag
     2401 cgattcagcc ctaataacct tgcaataaac ccagcctttt tgcctgggtt ttgttccacc
     2461 ttctcgaaga tttcctccag ctttttctct tcaattttac gtgccatttc tgcctccgta
     2521 aagtagattt gtgtccactt tacgagagac atgttgcaag taatgcaaca ggctacaaga
     2581 tgattaaccg tgcaaaaata cctttttcaa tgtttccggc aattccccgg aatacgacaa
     2641 caccaatcgc tgcccggccc gggtcgctgc catatacaac ttgcgggtat tgtcacggat
     2701 caattcctcg cgctcatcgt cagcaagccg caaagattgc tcttcttcaa acagcgcctt
     2761 caaccccacc agaaagacga tctgactctc caaccctgcg ccggcattga aggtcgtcac
     2821 acggatatag tcaccaggaa aggtatcctt cggatcgatc gccgagcctt ttccgaggcg
     2881 atcttcaagc gcctggatca aatgctgcac cccgttgcca tccgtgtgca aaagcaaaat
     2941 atgtttcctg ggcaagccct gctttaccag gttctccact tcatttgcga tgcgtgccac
     3001 ttcatcctgc gggctggtga gcgggatcaa ttcaggaaac gcccctttgg gcatgttcag
     3061 gatatccggt gtgaggatat cctcatcctt ctcgttctcc agacgcaggc ggtacaacaa
     3121 ggtggcgaac tgcatgatct cacgcgtggt acggtaactc ttcttcaagg tatgagtgtg
     3181 tccgcgcgca tccaacccca aagacctcca ggacgtgccg cggcctagaa atccctgggt
     3241 ggggtcggct accaaaaaga gatgtccatg ggggtttaac gccttttgga tcaaatgcat
     3301 ccagagcggc gcaaaaaatt gtgcttcatc gattaggatg aaatcatatt ccggcaattg
     3361 atgggcactt tccgtaacgg ctttccacaa ccgctgcgga acatcgcccc aatccaattc
     3421 gcctttcgct ttgagggagg tctgataggc taaaaaggca ttgaacatcc gcccacgcat
     3481 ttcctcgctc aggccaaatc cacgaccgcg ccggtcggtg gtgagatatt cggtccgtct
     3541 taaaggaagt tgatccttga accagtccaa ttcgctttgg aacatctttt gggtgatctt
     3601 cgtgtctctc aaatgactgc gccagatctc attcaggcga tgttccctgg cagacaggga
     3661 gagaggtgac acccaagccg gcttattcat ccaatgatga tgacaccagg cactaaacgt
     3721 ccgccattca atgtggttga cctcccgtcc catcaggcgg gtaaagcgtc cctgcatatc
     3781 atgactgatc ggacgattat gggtaaggac caaaaaacgc ttatccgggt ataggtgata
     3841 gagcaaacgc agacgataca acaggatcaa ggtcttgccg ctgccagcca ccccattgat
     3901 gatattgagg cgcatgtccg ctggaagcgc ctgatcatcg ggcggtaatt ccaactcgta
     3961 tttaaccgcc cgttcctgat cgtaatccag caggtaatca gtcaatcctg cttcgcggcg
     4021 gcgtgtccct tccagacgca cggtcatgtc agcaggaaca accacttccg gtgtgaaacg
     4081 ctgacgcagt ttctccagcc agatcgcatc catcggagag gacgggagaa aagcatccca
     4141 atttgattca ggctgcacta tctctttgcc tacccattgc ggctcccctt ccccgcgggc
     4201 aagccggctg gcttgaacat gcttgtccgg gatattcggg aagatcacaa gagtctccac
     4261 atgttgatcg ggcggaatgc ccaggctgga aacaaaggca tgcaataatt tattttcctg
     4321 cacgcccagt ggttcttttt cctcccccaa taacagcatt tgagaggccg tgcgggtctt
     4381 ttcctcggtg gacagactga ccttgaccag cagcgcctga ccagtttcat tgaagatcaa
     4441 aaaatccggc atgtccacct gccagggagc caggtgatgc cagatgaaat aagaatcagg
     4501 cagggttttt aaggcgcgat agacacgcag gacttccttc gggataagtt gcggcggggt
     4561 atctggaagg atgtgcgcca tggctcaagt gagattcccg tcaggtacag tgatgtgcgt
     4621 actaccctgg atcaggttgc gcagaagatc cagagtttct tttttactgg cgtcatactc
     4681 atcggctgcc agcatgtgat gactcccaac caggatcaat ttggaacgcg ggcgggtaac
     4741 cgcaacgttc agacggttgg gactgaaaag gaaatctgcc atttgcgctg caaacttggg
     4801 gctggcggtc gtaaaagaaa ccaggatgac ttctcgttcc tgcccttgca ttcgttccac
     4861 ggtatcaaca acgatctgat cgaatacgtc cccatcctca agagtacgcc gcaacagact
     4921 gcgaatcaac cggctttgag cacgatacgg aaccaccaca ccgatctccc tgggatggac
     4981 ttcgcgtgta agcagggaga ggatcaactc cagcacaaca ccagcctcca aacggctgcg
     5041 gactgtggaa tttcgatgac atagatcaag gaagacgatg ggagatgcgg gatcaaggac
     5101 catgtcccat ggagtcgcct ccggcgagag gtccagtcgg cgaggcgctg cttgtgggct
     5161 tgagcgtaat tcgtcacgat aaaagtttcg gctgggccag cgcgtcagga cgtcattcaa
     5221 gcgataggtg atattaagca tggtctcgtt gccatgcccg gaaagataat tgaagatcga
     5281 attttggact gcctcttcct cggaaaagat ggtgaccggc ggcagctggt tctcatcccc
     5341 aataaaaatg tatttactcc cagcgagcat gcccatgatg gcaagcggca gtgtgatctg
     5401 agaagcttca tcaaagagca ccacatcgaa ctcaaccccg gaaagccggt tggtctggct
     5461 tgcaaagggg gtggcgccga tgacataacc accgttcatc tctccaaaac gggatgcgcc
     5521 gaagtttata tagttgggca cgtccagatc agaaatactt ttatcttccc cgattttaca
     5581 gatggggaga gagggatcaa cctgattgat cttgttgagg gcattgtgta tggcgcgatg
     5641 cgtaagggca gtaacaaac
//
LOCUS       DAOQNJ010000105         5600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8474_length_5600_cov_7.498828, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000105 DAOQNJ010000000
VERSION     DAOQNJ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8474_length_5600_cov_7.498828"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLG52_04520"
     CDS             <1..719
                     /locus_tag="PLG52_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNB40740.1"
                     /translation="RREGYEFQVSRPEVIYHKADDGVLLEPYEEVHIETSNETVGPVV
                     EMLGARRGRMMDMQDSGQGTTRMVYIVPTRGLLGFRYQFLTSTRGAGVMNTIFHSYDE
                     LAGAMNTRQSGSLVAWEPGTTTAYALKSAEERGTLFVGPGVDVYEGMVVGENARAADI
                     SVNVCKKKHLTNMRASRADMEIRLTPPRQMSLDEAIEYLADDELLEVTPATFRIRKRI
                     LNTEERGKQTKKAKEQFGEE"
     gene            complement(766..1467)
                     /locus_tag="PLG52_04525"
     CDS             complement(766..1467)
                     /locus_tag="PLG52_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNB40741.1"
                     /translation="MKQSVYLFLITSLLLTSCSMSSSDAPSPEVATAAAMTVQAAIEG
                     NQTPLASPTSAGQSTPESTGNPMASFEDVTNCRVGPGVNYERVTQIQPGFAVEIVGYY
                     PPNYWIVQTDKGPCWVAGEFVTPSGSVSAVPTVTAPPTPTGGAPENVSLQKWDVACDY
                     ATNEAKVTIAWKDEDDEIGYRVVRNDVVIAELPANTTEFKETITLISGQTAGYSIISF
                     NATGSTQSQTISLGC"
     gene            complement(1492..2655)
                     /locus_tag="PLG52_04530"
     CDS             complement(1492..2655)
                     /locus_tag="PLG52_04530"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNB40742.1"
                     /translation="MPFYHKLGKIPHKRHTQFKKPDGKLYREELMGLEGFSSLQSILY
                     HHFLPPRVKKTADLGSAKPEYVDYGAIRHRAFKTAPTPLGADPVSGRIPLLGNNDVIL
                     GVSRAPKGKMNFFYRNSQAYETWWVHDGSGTIYSQFGKLPFRKGDYIVIPFGTTWQME
                     LDEETKFFTIENPSQIEPPKRYRNHYGQLLEHAPYCERDIRIPMELETHTERGEFEVR
                     VKVRDHISQHILDYHPHDVIGWDGYLYPWIFNIEDFEPITGRIHQPPPVHQTFEGWNF
                     VVCSFVPRLFDYHPEGIPAPYNHSNIQSDEVIYYAEGNFMSRKGIDRSDISLHPFGLP
                     HGPQPGMTEASIGKTETKELAVMVDTFHPLHLTKQALEMEKPYMETWLDGDGE"
     gene            complement(2665..3492)
                     /locus_tag="PLG52_04535"
     CDS             complement(2665..3492)
                     /locus_tag="PLG52_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40743.1"
                     /translation="MKLHMLVTGISLVVLVGCAPTATATESPSPTPLAVEPSATNPPT
                     ETETVSPSAFEAENISTFNELGIAIGFNYPEGFSQSISTSFNTTYSPVAPYDLPYPQN
                     AQIIFTDYSGGDGFTINGLRVFLADEVNALETGAVESVNAVLEGEADHHVDFPRLAGA
                     GSIIDAQVLPLAFQNGNGYRYLVAKSFSADPISSTKLTYMYQGVTNDGKFFVSFIMLV
                     DAPFLSEYVGQTLTTPEEFKTYFQDVNNLVETSSGDQFTPSLNALDELISSIVILEK"
     gene            complement(3506..3895)
                     /locus_tag="PLG52_04540"
     CDS             complement(3506..3895)
                     /locus_tag="PLG52_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB40744.1"
                     /translation="MTETVITVRPQGTHLSKQQLPNFEGISAKTAGSKHLCMHLVVIP
                     PGGKAVAHYHDGYETVIYIIEGKAETRYGHNLEHSTINEAGDFLFIPPNLPHQPVNLS
                     DTEQVIAVVARNDPNEQESVVVYEVKE"
     gene            complement(3904..4776)
                     /locus_tag="PLG52_04545"
     CDS             complement(3904..4776)
                     /locus_tag="PLG52_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40745.1"
                     /translation="MNKKIITPLICLAIVALACGGTVPSASQPNLETVVAATLQAITQ
                     QAANNPAATSQPEATGISVSFQNVQFVIPEGLASGASSEVIPAADEASGGPWGVAPEY
                     IEFVLMDYSPRDEFRPPVIRIYPAQEYAAVNSWAQSSLAKLQGILASPSMPLTNENLP
                     TIPFNGAAAQQYAAQAKIISFANGSGVRMTSQYAQFPGPILKDGSYYHYEGLTNDGKY
                     MVAIMVPVTLPIQATAENPSADGVVYPSDISDTIALDAYFQGVTDLLNAATPDSFQPN
                     LNVLDALIQSIQIK"
     gene            complement(4789..>5600)
                     /locus_tag="PLG52_04550"
     CDS             complement(4789..>5600)
                     /locus_tag="PLG52_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40746.1"
                     /translation="APAATPDVAAIVTQTLQALTAAPTEAQQQQAEVSPTAESSQQLT
                     PDGAPVDTAEISFFIPNGVANDASSTMTANVEYPYINPSLGDMPQHVSIALNLYAITE
                     SIHEPRILIFRASEYSQYSESNAATISQLQALLHVDGQPLQESLGADFSAQIHAVNFK
                     NGHGVRYLTQIFQNLNTVNNEELFYYYQGVTDDGHYFVQAILPINTSYLAADSNPNGP
                     LPADGIPFNIDDFPGYLNTVNQKLNSTDTFNFNPYLDALDEMIESIQIKGF"
BASE COUNT         1389 a         1213 c         1448 g         1550 t
ORIGIN      
        1 tgcgccgtga aggttatgaa tttcaggtct ctcgcccaga agtaatctat cacaaggcag
       61 atgatggtgt tttgcttgaa ccgtatgaag aagttcatat tgagaccagc aatgaaaccg
      121 tcggacccgt agtggaaatg ctcggtgcaa gacgcggcag aatgatggat atgcaggatt
      181 caggtcaggg aacgacccgt atggtgtaca tcgtgccgac ccgcggtttg ctcggcttcc
      241 gctatcaatt cctgacgtcc actcgtggtg caggtgtcat gaacaccatt ttccatagct
      301 atgatgaact tgccggagct atgaatactc gtcaaagtgg ttcgttggta gcatgggagc
      361 ctggcacgac cacggcttat gcattgaagt ctgctgaaga acgcggcacc ttgttcgttg
      421 gaccgggcgt tgatgtttac gaaggcatgg tggtgggcga aaatgcccgc gccgcagata
      481 tctctgtcaa tgtctgtaaa aagaagcatt tgacgaatat gcgcgcctca cgtgcggata
      541 tggaaattcg cctcactccg ccccgacaaa tgtctctcga cgaagcgatt gaatacctcg
      601 ctgacgacga attgctcgaa gtgacgcctg ctacattccg cattcgcaaa cgcattttga
      661 acaccgaaga gcgcggcaag caaacgaaga aggcaaaaga acagttcggc gaagaataaa
      721 aataaaagag ccatgcaatt tgcatggctc tttttgattc acaaactaac agcctagtga
      781 gattgtctga ctttgcgtag atcctgttgc attgaaggag ataatgctat acccagctgt
      841 ttgacctgag attagcgtga tggtctcttt aaactctgtt gtatttgcgg gtaactcagc
      901 gatcacaaca tcattgcgaa cgacgcgata gccaatttca tcatcttcat ctttccacgc
      961 aatcgttact ttggcttcat ttgtagcgta atcacaagcg acatcccatt tttgcaagct
     1021 gacattttct ggtgctccac cggtgggtgt tggtggtgcc gtaaccgttg gaaccgcgct
     1081 aacactccct gatggggtga cgaattcgcc agctacccaa caaggtcctt tgtctgtttg
     1141 aacgatccag taattaggcg ggtaatagcc cacgatttct accgcaaacc ctggctgaat
     1201 ctgggtgaca cgctcgtaat ttacaccggg tcccacacgg caattggtga catcttcaaa
     1261 gcttgccata gggtttcccg tgctttctgg tgtggactgt cctgcgctgg ttgggctggc
     1321 aagtggggtt tgattacctt caatagcggc ttgaactgtc atagcagcgg cagttgctac
     1381 ttctggggaa ggggcatcag aactagacat gctacaagat gtgaggagca aactggttat
     1441 gaggaataag taaactgatt gtttcattgt attctccgat gtaaattatc tttattcgcc
     1501 gtcaccatct aaccaggttt ccatgtaagg cttttccatt tcgagagctt gtttggtcaa
     1561 atgaagcgga tggaaagtat cgaccattac ggcgagctct ttcgtctctg tctttccaat
     1621 gctggcttct gtcataccgg gttgtggacc atgcggcaac ccaaatggat gtaaactgat
     1681 atcagaccgg tctatgcctt tgcgagacat gaaattgcct tctgcgtagt agatgacttc
     1741 gtcagattgg atgttggaat gattgtatgg cgcaggaatg ccttcggggt gataatcaaa
     1801 gagacgtggc acaaaagaac atacaacgaa attccagcct tcaaaggttt ggtgaactgg
     1861 cgggggttga tgtatacgcc cggtaatagg ctcgaaatct tcaatgttga aaatccaggg
     1921 atataagtat ccatcccagc caatgacatc atgaggatga tagtctagga tatgttgcga
     1981 gatgtggtcg cgtactttga cacgcacttc aaactcacca cgttcggtgt gagtttctaa
     2041 ctccatgggg atgcggatat cacgctcaca ataaggggca tgttcgagca gttgaccgta
     2101 gtgattgcgg tagcgcttgg gcggctcgat ctggctaggg ttttcgatgg tgaagaactt
     2161 tgtttcttcg tctaattcca tttgccaagt tgtgccaaat ggaatcacga tgtaatcgcc
     2221 ttttcgaaag ggtaatttgc caaattggga atatatggtg ccgctaccat catgaaccca
     2281 ccaagtttca taagcttgtg agttgcggta gaaaaagttc atttttcctt taggtgcccg
     2341 tgaaaccccc aagataacat cgttatttcc aagcagagga attcttccag aaactggatc
     2401 tgccccgagc ggagtgggag ccgttttgaa cgcacgatgg cggatcgctc cgtaatccac
     2461 atattcaggc ttggcagacc caagatccgc tgtctttttt acgcgcggcg gcagaaagtg
     2521 atgatagagg atcgattgca gcgatgaaaa tccttcgagc cccatcagct cttcgcgata
     2581 aagttttcca tctggttttt tgaattgtgt gtgacgtttg tgcggaattt tcccaagttt
     2641 gtgatagaac ggcatgtctt ctccttattt ttctaaaatg acaatcgaag aaatcaattc
     2701 atccagcgcg ttaagtgaag gagtaaattg gtcgccgctg gaggtttcta caaggttatt
     2761 cacatcttga aaataagttt taaactcttc gggtgttgta agagtttgcc caacatattc
     2821 actaagaaaa ggtgcatcaa ctagcatgat gaacgacaca aaaaactttc catcatttgt
     2881 aacgccctga tacatataag ttaacttcgt gctgctaatt gggtctgctg agaaagactt
     2941 tgccacaaga tagcgatagc cattgccatt ttggaatgcc agagggagca cttgcgcatc
     3001 gataatgctg cccgcacccg ccaagcgcgg aaagtcaaca tggtgatctg cttcaccttc
     3061 aaggaccgca ttcacactct ccacggctcc ggtttccaaa gcgttaactt catctgccag
     3121 aaaaacacgt agtccattta tagtgaaacc atctccacca gaataatcgg tgaagatgat
     3181 ctgagcattt tgaggatatg gcaggtcgta aggagctacc ggcgaatagg ttgtgttgaa
     3241 gctcgtgctg atgctttgtg agaagccttc aggataatta aaaccgattg cgatgcccaa
     3301 ctcattaaac gtggaaatat tttccgcctc aaacgcagac ggtgaaactg tttcagtttc
     3361 tgtaggtggg ttcgtcgcag acggctccac agctagaggt gtagggcttg ggctttcggt
     3421 tgccgttgct gtcggtgcgc aaccgaccag aactaccaat gaaatgcctg taacaagcat
     3481 atgaagtttc attttcacct gctttctatt cttttacttc atatactaca acactttcct
     3541 gctcatttgg gtcgtttcta gcgaccacag ctatgacttg ttctgtatcg ctaaggttaa
     3601 cgggttgatg cggtaaattt ggcgggatga aaagaaagtc gcccgcttcg ttaattgtgg
     3661 agtgctcaag gttatgccca tagcgcgtct cggctttccc ttcgatgatg tagatcacag
     3721 tctcgtagcc atcatgatag tgagctaccg ctttcccacc tggcgggatg acgacaaggt
     3781 gcatgcaaag atgtttggac cctgccgtct tcgcagagat gccttcaaaa tttggtaact
     3841 gttgtttgct gaggtgagtc ccttgtgggc gaacggtgat cactgtttcg gtcatgatgc
     3901 tccttatttg atttggatgg attgaatgag cgcgtctaaa acgtttaaat tcggctgaaa
     3961 tgaatctggg gtagccgcat ttagcaaatc agtgacccct tggaagtatg cgtccagcgc
     4021 gatggtatct gaaatatcgc tggggtaaac aacaccatcg gcagaggggt tttctgcggt
     4081 agcctgtatg ggcaatgtga cgggcaccat gattgcaacc atatacttgc catcattggt
     4141 gagaccttca taatgatagt agctgccatc cttcaaaatg ggtcctggaa actgagcata
     4201 ctgtgaagtc atgcgaacgc cgcttccatt agcgaacgat atgatctttg cctgagctgc
     4261 atattgttgg gcggcagcac cattgaaagg aatggtggga aggttttcat tggtcaacgg
     4321 catggaggga cttgccaaga ttccctgtag ttttgccaaa ctgctttgtg cccatgaatt
     4381 tacggctgca tattcctgtg ccggataaat gcgaataacc ggtggtctga attcatcgcg
     4441 tggtgaatag tccattagga caaattcaat gtactctggt gccacgcccc agggtccacc
     4501 agaggcttca tctgcggcag ggataacttc actggatgcc ccgcttgcca atccttccgg
     4561 aatcacaaac tgcacatttt ggaatgacac agagatgcca gtggcttctg gctgactggt
     4621 tgcggcagga ttgtttgccg cttgttgcgt gatggcttgt aaggtggcgg caactacagt
     4681 ttctaagttt ggctggcttg cagagggaac tgtccctcca caggcgagag cgacaattgc
     4741 cagacaaatc aaaggagtga taattttttt gttcattgat attccttttt agaaaccttt
     4801 gatttgtatg gattcgatca tttcatccaa tgcatcaagg taggggttga aattgaaagt
     4861 atctgtgctg ttgagctttt gattgaccgt attgaggtat ccaggaaaat cgtctatatt
     4921 gaatgggatt ccatccgcag gtaagggtcc atttgggttg ctgtctgcgg ctaggtaaga
     4981 agtgttaatg ggcaagatag cttgtacaaa gtaatgccca tcatctgtga cgccttgata
     5041 atagtagaaa agctcctcat tgttcacggt attaaggttt tggaatattt gtgtcaggta
     5101 gcgcacacca tgtccatttt tgaagttgac tgcatggatt tgagcactga aatcagcacc
     5161 gagactttcc tgcaatggct gcccatcaac atgtagcaag gcttgtagtt ggctgatagt
     5221 tgcagcattg gattcactat attgtgagta ttctgaggca cggaagatca aaattcgtgg
     5281 ttcgtggatg ctttcagtga tggcatacaa atttagggca atagaaacat gttggggcat
     5341 gtcacctaaa gatgggttga tgtagggata ttccacgttc gcggtcatag tagagctggc
     5401 gtcgttggcg acaccatttg gaatgaagaa agagatttct gcggtatcta cgggtgcgcc
     5461 atcgggtgtg agttgttggg atgattcagc ggttggcgag acctcggctt gttgttgctg
     5521 cgcctcagtg ggggcagccg tgagtgcttg caaggtttgg gtcactatag cggctacatc
     5581 gggcgtggcg gcgggagcgc
//
LOCUS       DAOQNJ010000106         5575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8538_length_5575_cov_10.632246, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000106 DAOQNJ010000000
VERSION     DAOQNJ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8538_length_5575_cov_10.632246"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="PLG52_04555"
     CDS             complement(<1..1358)
                     /locus_tag="PLG52_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNB40747.1"
                     /translation="MLTELHIQNFAIIDKLDLRFGPGLIILTGETGAGKSIILDAVVM
                     LIGGKADTTFVRSESDAAFVEGVFQLKGPEREAVHEVLNREELMDDPDYVVLMREVRK
                     EGRSVARINGRTVNVGLLKELGALLIDIHGQAEHLSLLDPRAHLGLLDRYADVAKPLN
                     DYRQTYHSLLTLRHELNDLRKAQADSDRRIEMLTYQVEEIESAKLKVGEDEELRKERD
                     RLANAESLAQNAQEALAILEEGSPETPAATDLIGQAAQALTALAKIDAGQSELANQAE
                     VMLDTLSDIIHEVRNYLEEIEFNPKRLDDVEERLDLIHSLTRKYGGNIPAVISYGADA
                     KKQLETITGAADRIAELEMQEAKFLEKLSKQGNILSEKRKSAAETMSKGIELELDDLR
                     MASAQFGVDFQTKADPNGIPVADGTRLAFDQNGIDRIEFLVAPNPGEGLKPLAKVASG
                     GET"
     gene            complement(1362..1748)
                     /locus_tag="PLG52_04560"
     CDS             complement(1362..1748)
                     /locus_tag="PLG52_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40748.1"
                     /translation="MRERQKSFDTALWYDFALGFVVAGLLSGVASFLVTLIGAIGFIG
                     FMLVFIGSSAAGGIIAEAVRAVTRRHRSRALFMTVGAGVIAGALPMLLVQLLSVNVFG
                     LIFQGIYLVIAVPLVYARLSGIQLTR"
     gene            complement(1883..2746)
                     /locus_tag="PLG52_04565"
     CDS             complement(1883..2746)
                     /locus_tag="PLG52_04565"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNB40749.1"
                     /translation="MSSPFIPKRIVVTAYPKMPEAFTEAAAMSVFLKEKGIDAPVGSL
                     YDEELRKRVKQGEFDMLIAVGGDGSVLRAGHLCAPSGVPILGVNLGRLGFLIQIDRKE
                     WRDYFMRLLAGEAWIENRMMLRAEHIRAGEKLGSSLALNEVVVGRGQNLRPVRLTAYV
                     DKRELTSYVADGLIAATATGSTAYALAAGGPILPPELRNILLVPIAPHLSVDRAVVLS
                     EGTSVDIVMKSENAIFSVDGQPPTPLMEEDHVNVTATDVTAQFVRFGNPGYFYRNITM
                     HINENSSGFPR"
     gene            complement(2784..3296)
                     /gene="cas4"
                     /locus_tag="PLG52_04570"
     CDS             complement(2784..3296)
                     /gene="cas4"
                     /locus_tag="PLG52_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNB40750.1"
                     /translation="MTSIVLILFLLALMFFWQSNRQQKAAGLPGGRVIYTDTRGWGKV
                     EKPLFYATLGLTGKPDYLIEKNGQIIPVEVKSGRAPEAPYDSHIFQLASYCLLVEKTY
                     GRRPPYGIIHYENRDFAIDYTRELETALIELIADMREDDVRRDVARSHEQASRCKRCG
                     YHDICEQRLA"
     gene            complement(3293..3529)
                     /locus_tag="PLG52_04575"
     CDS             complement(3293..3529)
                     /locus_tag="PLG52_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40751.1"
                     /translation="MRPIRSSEIGSYLYCRRAWWYRKIGTPSENKAELAAGTELHRKH
                     GRQVLASALTRTLGWLLLLAAILTLVAYLAFQVL"
     gene            3628..4179
                     /locus_tag="PLG52_04580"
     CDS             3628..4179
                     /locus_tag="PLG52_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNB40752.1"
                     /translation="MTKQDTYSIDIAGVKRELRLFEIKPGLRIAILNILGDTELVQAC
                     ARGLADKVKNVEYDIIVTAEAKSIPIAHALSVETKKPYVVLRKTYKPYMGDALKAETL
                     SITTGQPQVLILDEKDRELIQGKKVLVVDDVISTGSTLQGMRMILDKANATVAGEAAI
                     LTEGDRSQWMNIHSLGHLPLFTD"
     gene            complement(4208..4450)
                     /locus_tag="PLG52_04585"
     CDS             complement(4208..4450)
                     /locus_tag="PLG52_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB40753.1"
                     /translation="MSCFCYILECADGTFYTGWTTDPERRVSQHNKGVGARYTRTRRP
                     VRLVYLEEQPDKITALKRERAIKALSRKKKMELFMM"
     gene            complement(4447..5436)
                     /locus_tag="PLG52_04590"
     CDS             complement(4447..5436)
                     /locus_tag="PLG52_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNB40754.1"
                     /translation="MPVKITIIGLGQIGASIGLALAAHKDRVVTTGHDKEYSVEQRAK
                     KLGVVNETNHNLPSSVENADLVILALPLNEIRETLGHIAQDLRQDVVVVETAPVKAEV
                     TKWVKEILPETAHYVGVVPAIGPAYLDVTASGLDSAKADLFERGIFLVSAPSGTSGEA
                     VKLVTDFVKLLGSTSILTDVVESDGLMASSYILPRLASVALLNGTMSQPGWNEVKKSA
                     SRGYFAATSALLDNSLEGLAMLAEHNRANTIHSLNVMINSLLDLRDDLENEDHASFVK
                     RLKSAKEGRESWLVEREKADWSRIPGEEIEKPSFMESLFGSKLGKLGKDKGKG"
BASE COUNT         1350 a         1395 c         1472 g         1358 t
ORIGIN      
        1 gtttcaccac cagaggcaac ttttgccaac ggcttaagcc cttcgccggg gttgggggca
       61 acgagaaatt ctatgcggtc gataccgttc tggtcgaagg cgaggcgggt tccatccgca
      121 acaggaatgc cattgggatc tgccttggtt tggaagtcca cgccgaattg agcagatgcc
      181 atgcgtagat catccaactc cagttcaatg cctttgctca tggtttcagc cgcagacttg
      241 cgtttttcag aaaggatatt gccttgcttg gaaagtttct caaggaactt ggcttcttgc
      301 atttcaagct cggcaatgcg gtctgctgcg ccggtaatgg tttcaagttg tttctttgcg
      361 tctgccccgt atgagatgac agccggaata ttgccgccat acttgcgggt cagagagtgg
      421 atgagatcca ggcgttcttc gacatcatcc aaacgcttgg ggttgaattc gatctcttct
      481 aaataattac gcacttcatg aatgatatct gaaagcgtat cgagcatcac ttcggcttga
      541 tttgccaatt cagattgacc cgcgtcgatc tttgcgagcg cggtcaatgc ctgagccgcc
      601 tgaccgatca aatcggttgc ggcaggggtc tcgggtgagc cttcttcaag aatagctaaa
      661 gcttcctgcg cgttttgtgc cagtgattcg gcattggcaa gacggtctcg ttctttgcgc
      721 agttcttcat cttcgcccac tttcaacttg gcagattcaa tttcctctac ctgataggtg
      781 agcatttcaa tgcggcggtc tgagtcggct tgggctttgc gcaggtcatt cagctcatgg
      841 cgcagggtca acagcgagtg ataggtttgg cgatagtcgt tcaatggctt ggcgacatcg
      901 gcatagcggt cgagcaagcc aagatgcgcg cgcgggtcga gcagggagag atgttctgct
      961 tgcccatgaa tatcgatcag gagcgcgccc aattctttga gcaagcctac attcaccgtc
     1021 cgcccgttga tgcgtgccac gctgcgtcct tctttgcgga cttcacgcat gagcacaaca
     1081 taatcgggat catccatcaa ttcttcgcgg ttgaggactt catgcaccgc ctcacgttct
     1141 ggacctttga gctggaacac gccttcaaca aatgcggcgt ctgattcaga gcgcacgaag
     1201 gtcgtgtcgg ctttgccgcc aatgagcatg accacagcat caaggatgat ggatttgccc
     1261 gcgccggttt caccggtgag aatgatcaaa cccggaccaa aacgcaggtc gagtttgtcg
     1321 atgatcgcaa aattttggat gtggagttct gtgagcatag gttatctagt gagttgaata
     1381 ccggaaaggc gggcgtagac taaaggaaca gcaataacaa gataaatgcc ttggaagatc
     1441 aacccaaaga cattgacgct caaaagttgt acaagaagca tgggcaaggc acccgcaatc
     1501 acaccagcgc caactgtcat gaaaagcgcg cgcgagcggt gtcggcgagt gacagctcga
     1561 acggcttcag cgattatgcc tccggcggcg gaagagccga tgaaaacaag catgaaaccg
     1621 atgaagccaa tcgcaccgat caaggtcact aagaaggatg caacgccaga gaggagtccg
     1681 gcaacgacaa agcccaaggc aaaatcatac caaagagcgg tgtcgaaaga cttttgcctt
     1741 tcgcgcatgc actctttgca aatatagccc gtaggggtgc tgacggcaca tgaggtgcag
     1801 atgtaacgct cacagcgttt gcaacgcaga gaggtttctc tgttcgggtg aacataacaa
     1861 gtagttgtgg tggagaattc ggtcatcgtg gaaatcctga agaattttcg tttatgtgca
     1921 tggtgatatt tctgtaaaag tatcctgggt ttccaaatcg tacaaattgt gcggttacat
     1981 cggtcgccgt gacgttgaca tggtcttctt ccattaaggg tgtgggtggc tgaccatcca
     2041 cactgaaaat ggcgttctca cttttcatta cgatatctac agatgtgcct tctgaaagaa
     2101 cgacagctcg gtctacagaa agatgcggcg caatgggaac caataaaata ttgcgcaatt
     2161 cgggcggcaa gatcggtccg cctgcggcga gggcataggc tgtggagccg gttgccgttg
     2221 ctgcaattaa accatcggcg acataactgg tgagctcgcg tttatcgaca taagcggtca
     2281 aacgcaccgg gcggaggttt tgtccgcgcc cgacgacgac ttcattcaac gccagtgacg
     2341 aaccaagttt ctcaccagca cggatgtgtt cagcgcgcaa catcatacga ttttcgatcc
     2401 atgcttcgcc agcgagcaac cgcataaaat aatcgcgcca ttctttgcgg tcgatctgaa
     2461 tgaggaaccc gagcctgcca agattcactc ccaagatcgg aacgcccgat ggggcacaca
     2521 aatgcccggc tcgcaagacg ctgccatcgc cgccaacggc gatcagcata tcaaactcac
     2581 cttgtttcac ccgcttgcgt agttcttcat cgtacaacga accgacagga gcatctatgc
     2641 ctttttcttt gagaaaaaca gacatggctg ctgcttcggt gaaggcttcc ggcattttag
     2701 gatacgccgt gacgacgata cgctttggga tgaaaggcga agacatgccg gaaattataa
     2761 ccttttgtct ttgctaacgg atgttatgcc agccgttgtt cacaaatatc atgatacccg
     2821 catcgcttac aacgggacgc ctgctcatgc gaccgggcca catctctgcg gacgtcgtct
     2881 tcgcgcatat cggcaatgag ttcaatcagc gctgtttcca gttcacgagt gtagtcaatg
     2941 gcaaagtcgc ggttctcgta gtggatgatg ccatagggtg gacgcctgcc ataggtcttt
     3001 tcaacgagca gacaatagga agctaactga aaaatatgcg agtcatacgg cgcttcaggc
     3061 gcacgccctg acttaacctc cacggggatg atttgtccgt tcttttcgat caaataatcg
     3121 ggtttgcctg tcagcccgag ggttgcatag aacagcggct tttccacttt gccccaacca
     3181 cgcgtgtcgg tgtagatgac gcgtccgccg ggcaagccgg cggctttctg ctggcggttc
     3241 gattgccaga agaacataag cgcaagcaaa aaaaggataa ggacgatgga tgtcatagca
     3301 cttgaaaagc aagatacgct accaaagtaa gaatcgctgc tagaagcagg agccagccca
     3361 aggtccgcgt gagagccgag gcaaggactt ggcgtccgtg tttgcggtgg agttcggttc
     3421 cggcggcaag ctccgctttg ttctcggagg gtgtcccgat cttgcgatac caccaggcgc
     3481 ggcgacagta gaggtaagaa ccaatttcag aggagcggat cggtcgcatt tgggtagtat
     3541 agcacaaatt ttctaattga attgaggcgg gaacataatc gaatcgtgat aaactttcgg
     3601 tgcaaaattt ccccgtggag acgtctgatg acgaaacaag acacttactc aatcgatatt
     3661 gctggcgtaa aacgcgagtt acgcttgttt gaaattaaac cagggttgcg cattgcgatc
     3721 ctgaacattt tgggcgacac cgaattggtg caagcctgtg cccgcggact tgccgataag
     3781 gtgaagaatg tcgagtacga cattatcgta accgctgaag cgaagtccat tcccattgcg
     3841 catgcgcttt cggtggagac caagaagccc tatgtggtgt tgcgcaagac ctataaaccg
     3901 tatatgggcg atgctctcaa agcggagacg ctttcgatca ccacgggtca gccgcaggtt
     3961 ttgattttgg atgagaagga ccgtgagttg attcaaggca agaaggtttt ggtagtggat
     4021 gatgtcatca gcaccggttc gaccctgcag ggcatgcgca tgatcctcga taaagccaat
     4081 gcgactgtgg ctggtgaagc ggcgatcctc accgagggcg accgttccca atggatgaat
     4141 atccattcgc tggggcattt acctttgttt actgattaaa tgcgtaaggg cgactcgcga
     4201 gtcgccccta catcatgaac aattccattt tcttttttct tgagagagcc ttgatggctc
     4261 tctctcgttt taacgctgtg attttatcgg gctgttcttc gaggtagacc agtctcacgg
     4321 ggcggcgtgt tcgggtgtag cgtgcgccaa cgcctttgtt gtgctgagac acgcgtcgtt
     4381 ctgggtcggt ggtccagccg gtgtaaaagg ttccgtcggc acattcaagg atgtaacaaa
     4441 agcagctcat cccttgcctt tatcttttcc caacttgcca agttttgagc caaacagact
     4501 ttccatgaaa gatggctttt cgatctcttc cccaggaatt ctcgaccagt ctgctttttc
     4561 tcgttcgacg agccaacttt ccctgccttc cttggcagac tttaatcgct ttacgaaaga
     4621 agcatggtct tcattttcaa ggtcatcgcg caggtcgagc agactgttga tcatcacatt
     4681 caacgaatgg atggtatttg cgcggttgtg ttccgcaagc atggcaagcc cttccaacga
     4741 attatccaat agggcagagg tggcggcgaa atatccgcgg ctggcagatt ttttcacttc
     4801 gttccaccct ggctgactca tcgttccatt caataaagca acagacgcca aacgtggcag
     4861 gatgtaagaa gaagccatca acccatcgga ttcaacgaca tcggtcagaa tggaggttga
     4921 gccgaggagt ttgacgaaat cggtcactag tttcacggct tcgccagatg tgccagaagg
     4981 ggctgaaacg aggaaaatgc ccctttcgaa gaggtcagct ttggctgagt ccagacccga
     5041 ggcagtaaca tccaaataag ctggtccaat cgcgggcaca actccaacgt aatgagccgt
     5101 ttctggcaag atttccttga cccatttcgt cacttcagcc ttgaccgggg cagtttcgac
     5161 cactacaacg tcttggcgca ggtcttgggc aatgtgccca agcgtttcgc gaatttcatt
     5221 gagaggtaaa gccaaaatga ccaggtcggc attttccacc gagctgggca agttatggtt
     5281 ggtttcattc accacgccaa gctttttggc gcgctgttct acactgtact ctttatcgtg
     5341 accggtcgtc accactctgt ctttgtgtgc ggcaagcgcc agcccaatcg aagccccaat
     5401 ttgccccaaa ccaataattg tgatctttac aggcatatca acctcaaatc tggaatggtc
     5461 gcattataac ccccgcttaa aatacgagaa ccgcggacgc cgaccgcggt tctctttgaa
     5521 aggaggagaa gagaaatcac cttacgcacg taaatttatc atggttatcc gaata
//
LOCUS       DAOQNJ010000107         5547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8611_length_5547_cov_9.355608, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000107 DAOQNJ010000000
VERSION     DAOQNJ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8611_length_5547_cov_9.355608"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PLG52_04595"
     CDS             complement(<1..1519)
                     /locus_tag="PLG52_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40755.1"
                     /translation="MVNINENLPKIKVSAPNYTWEWDNVTDTFWLHDKNQRLISRAQH
                     KPLCLTSPMPLEFTASTYEIKADQIHITYSGQNPASSLTVVWSFHPDFISLEPFHLTL
                     PAGVDLIRIIYFPEVDGDSYKPSMYSHYAVVPGLTMSTNISPIVDLHSRFNTTAVLGS
                     GAMRGPGLTQQWGLPAHYFCTFNTSDRWNAIGAKAQQSEAACWGLTALPQGDFRLEIR
                     EIGISPVLNLRSDLWKHKPDTFGFHFLITFGENYHEAIRKYYQVLQREKFITPKTHSA
                     KKKEVLLAPQYNTWGVESALALPSEQLTEDLVRDIFSKLQRSGMQAKTFVIDDKWEET
                     HGELKHDPKRFPNFENLLREIRDQGYNIGLWAAFLRCQNPAALGLDETHLLQTHEGKT
                     LWLDHQTSHYGIFDMTQPKVQEVLSQRAKEFIRHYQPDLIKFDFGYELPSLDVAAPKD
                     MTFAGERLLQKGLEVIVGAMKEENPDLVIMYYGLSPLLIDYYDLHSPDDLVYCIGD"
     gene            1614..2624
                     /locus_tag="PLG52_04600"
     CDS             1614..2624
                     /locus_tag="PLG52_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNB40756.1"
                     /translation="MTPNRPTIRDVARQAGVSHQTVSRVINGSEDVLPETRAIVEAAI
                     EELGYRPSAIARSMARGLTHTLAIISPNLTDYTFASVIEGAEVEARQHNYFVLSSSAS
                     DPQAFRGLVDELVGHRRVDGLIVINPYADERYQLMPKDFPVVFVGARSHDENVCSISL
                     DDDKVAYEATQHLISLGHKRIALVTGPMEEDCSQDRLAGFQRALNEAGIPFEQSLMFE
                     GDWSATSGSEALADFVKKGNLPTAVFAQNDRMAMGVMRAARDANLKVPDQLSVIGVDD
                     MPLSSYFDPPLTTMRQDMPLIGQEAIRKLIDIIQNKTVEQTVLKLPAQLVVRQSTVKG
                     GD"
     gene            2700..4097
                     /locus_tag="PLG52_04605"
     CDS             2700..4097
                     /locus_tag="PLG52_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNB40757.1"
                     /translation="MKKNLLVKIMGLLVIASFVLAACGTPAATEAPAETEAPAVTEAP
                     AATEAPATEASSADVALRWRTRPDNQEEIDVYSKISTELDAQLDGITLTYEPGGSEGA
                     NYQDQLRAEIAAGTAPDVFWIPGTDVADFATRGLILNAADLAAATEGFTATDFYEGPM
                     YHLTYNPESGASGDGSGAVWGLPRDVSTFALYLNLDLIAEAGAADPRELAANGEWDWE
                     AFLEVSQATRALGSDIYGYGASAWWGPYGVWLNASGGGFFTEDRAACNLNSEESLAGL
                     DFQRSLYQDYDVAVPYGEDPEPGFRAGKVAMFQNGRWATPGVRTVDFNWDVVELPAGP
                     NGTPGNWLFWGAYVINANTEHPEEAWALVQALTTAETQGKVAALGANIPSRVSQEALD
                     AFLTFTPPANNQAFLNGIAPNAAPTAEGPLWAGSWPEFDKIMGPAIQGVLTGSTTVED
                     FGATICDEANKAFNQ"
     gene            4245..>5547
                     /locus_tag="PLG52_04610"
     CDS             4245..>5547
                     /locus_tag="PLG52_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNB40758.1"
                     /translation="MMAQSRIDAQTNVPLRIQTLWRGLFALLAVASIGMIWTRTVETP
                     LWQKIVISIVALAAAVLSGYGAYQISKRNHAGRMTSLVLDYLGFVVCFVFALNVGEFF
                     VGIDALGENFGKGIPYLGITALGYFLGMLEEYFPTKNKGSESSLKTVSKWVMLGGFAL
                     FLWQVNALNGIIYFLGKLAQPSGMIAVVGLVIFGLSLWSMNRPDMAHALNVKTGHEQI
                     INGWLFLSPNLLGFLIFFAGPLLLSFYFSFTDSDAIRTPNWVGFENYAKILNIKFVTL
                     DSPDQLARDAVDIKVYDEVGRFTYGDTGILFAAEDKFFWLALRNTLTFVLFAVPLSVI
                     PALVLSNILNSKLPGMKFYRAVYFMPSIAAVVGISLVWQWLYNATIGYINYFITLGIE
                     FWNNLFNVAIIDPKIQWVSDEKTALFAIIIIAAWQTMGFNTV"
BASE COUNT         1236 a         1340 c         1483 g         1488 t
ORIGIN      
        1 aatcaccaat acagtaaacg aggtcatcgg ggctgtgcag gtcgtagtaa tcaataagca
       61 ggggagacag accatagtac atgatgacga ggtctgggtt ttcttctttc attgcaccta
      121 caataacttc caaccctttt tgcaacaagc gttcgcctgc aaaagtcata tcttttggag
      181 cagcgacatc gagcgatggc aattcatatc cgaagtcgaa tttgatcagg tctggctggt
      241 agtgccggat gaattctttg gcgcgctgac tcaacacttc ctgcactttg ggctgagtca
      301 tatcgaagat gccgtagtgg gaggtttgat ggtcgagcca gagcgttttg ccttcgtgtg
      361 tttgtaacaa gtgtgtctcg tccaagccaa gcgcggcagg attttgacaa cgcagaaacg
      421 ccgcccataa gccgatgttg tagccttgat cgcggatttc tcttaaaaga ttttcgaagt
      481 ttggaaagcg tttggggtca tgtttgagtt cgccgtgcgt ctcttcccat ttgtcatcaa
      541 tgacaaatgt cttggcttgc atgccagagc gttgaagttt gctaaaaatg tcgcggacga
      601 ggtcttcggt caattgctca gagggcagag ccagagcaga ctcaacgccc caagtgttgt
      661 actgtggcgc gagcagcact tctttctttt tcgcagaatg cgtttttggc gtgatgaatt
      721 tttcacgttg taaaacttga taatacttac gaatggcttc atgataattc tcgccaaagg
      781 tgatgagaaa gtgaaagcca aatgtatctg gcttgtgttt ccataagtcg ctgcgcaggt
      841 tgaggacggg actgatgcca atctcgcgta tttccaagcg gaagtcgcct tggggtaatg
      901 cggtgagtcc ccagcaggca gcttcgcttt gttgtgcttt tgcaccaatg gcgttccaac
      961 ggtcggaggt gttgaaggtg cagaagtaat gcgcaggtaa accccattgt tgagtgagac
     1021 caggtccacg catggcgcca gagccgagca cggcggtggt gttgaagcgt gagtgtaagt
     1081 ccacgatggg gctgatgttg gtgctcatgg tcagaccggg gacgacagca taatgactgt
     1141 acatggatgg tttgtaagag tcaccgtcca cttcggggaa gtagatgatg cgaataagat
     1201 ctacgcctgc tgggagggtg aggtggaacg gttcgaggga gataaagtct gggtggaagg
     1261 accagaccac agttaaggaa gaagcggggt tttgtccact gtaagtgatg tggatttggt
     1321 cggccttgat ttcgtaggtg gaggctgtaa attccagcgg catgggggag gtgaggcaga
     1381 gtggtttgtg ttgggcgcgg gaaatgagac gttggttttt gtcgtgtagc caaaatgtgt
     1441 ctgtgacgtt atcccattcc caggtgtaat ttggagcgga gactttaatt ttggggagat
     1501 tctcattaat gtttaccatc aaaacacctc aatctgaaat tgcctacttg acaaaggggg
     1561 tatttttctg tagactaatg ttaccgttca cgtgaccggt cacattatat caaatgactc
     1621 ctaatcgacc taccattcgt gatgttgccc gccaggctgg tgtttctcat cagaccgttt
     1681 ctcgtgtgat taacggtagt gaggacgtgc tgcctgaaac ccgcgccatt gtcgaggcgg
     1741 caattgagga attgggctat cgtcctagtg cgatagctcg gtcgatggca cgcgggttga
     1801 cgcataccct ggcgatcatt tctcccaatc taacagacta tacctttgcc agtgtgatcg
     1861 aaggtgctga ggtggaagcc cgccaacaca attattttgt gttgtcttcg tcggcttcag
     1921 atccgcaagc gtttcggggc ctggtggatg agctggtggg gcaccggcgg gtggatgggt
     1981 tgatcgtcat caacccgtat gctgatgaac gttatcagct catgccaaag gattttccgg
     2041 tggtattcgt gggggcacgc tcgcatgatg agaatgtatg ttcaatttca ttggacgatg
     2101 acaaggtggc ctatgaagcc acacaacatt tgatttcgct tggtcacaaa cgcattgccc
     2161 tggtcaccgg gcccatggaa gaagattgtt cgcaagaccg tttggcaggg ttccaacgtg
     2221 cattgaatga agctggaatt ccttttgagc aatcattgat gtttgaaggg gattggtcgg
     2281 caacttcagg cagtgaagca ttggcagatt tcgtcaagaa aggcaatttg ccaacagcag
     2341 tatttgccca gaatgaccgc atggcaatgg gtgtgatgcg tgcggcgcgg gatgcgaacc
     2401 taaaagtgcc ggatcaactc tctgtcattg gtgtggatga tatgccttta tcttcctatt
     2461 ttgacccgcc tcttactacc atgcggcagg atatgcctct cattggtcag gaagccatcc
     2521 gcaagttgat cgatattatt caaaacaaga ccgttgagca aaccgtgctt aaactacccg
     2581 ctcagttggt tgtacgtcaa tctaccgtca aaggaggtga ttgaacttcg taatactttt
     2641 agagtaaacc tgcctgatcg tacgatgatt tgttcgtcca attccaagga gaagaataaa
     2701 tgaaaaagaa tttgctcgtg aagatcatgg ggctgctcgt tatagcctct ttcgttttag
     2761 cggcttgtgg cacccctgct gcaaccgaag cccctgctga aactgaagcg ccagcagtga
     2821 cggaagcccc agcggcaact gaagctcccg ccaccgaagc atcgagcgct gatgttgctt
     2881 tgcgctggcg cacacgccca gacaatcagg aagagattga cgtttacagc aagatcagta
     2941 cagaacttga tgctcagctt gatggtatca cattaactta tgaacccggt ggttctgaag
     3001 gcgcgaacta tcaggaccaa ctccgcgctg aaatcgctgc tggaaccgct ccggatgtct
     3061 tctggatccc tggaaccgat gtggctgatt ttgccacccg cggtttgatc ctcaatgctg
     3121 ccgacctcgc tgctgccacc gaaggtttca ccgctacaga cttctatgaa ggtccgatgt
     3181 atcacctgac ttacaatccc gaaagcggcg cttctggcga cggctcgggt gcggtttggg
     3241 gtctgcctcg cgatgtttcc acctttgctc tttacctcaa ccttgatctg atcgctgaag
     3301 ctggcgctgc cgatccccgt gaactggctg ccaacggcga atgggattgg gaagccttcc
     3361 tcgaagtttc tcaggctacc cgcgctcttg gctctgacat ttatggttat ggcgcttccg
     3421 cttggtgggg cccttacggt gtctggttga atgcctctgg cggcggtttc ttcactgaag
     3481 accgcgcggc ttgtaacctc aacagcgaag aatcccttgc tggtctcgat ttccaacgct
     3541 cgctctatca ggactatgat gtggctgtgc cgtacggcga agatcccgaa ccgggcttcc
     3601 gcgctggcaa ggttgccatg ttccagaacg gccgctgggc aactccgggc gtccgcaccg
     3661 tggacttcaa ttgggacgtg gttgaattgc ctgctggtcc gaatggaacc cccggcaact
     3721 ggctgttctg gggcgcgtat gtgatcaacg ccaataccga acatcctgaa gaagcctggg
     3781 ctctcgttca ggcactcacc actgctgaaa cccagggcaa ggttgctgct ctcggcgcga
     3841 acatccccag ccgtgtgagc caggaagctc tcgacgcctt cctcactttc acaccgcctg
     3901 ccaacaatca agccttcttg aacggtatcg ctcctaacgc tgctccgacc gctgaaggtc
     3961 cgttgtgggc tggttcctgg cctgagtttg acaagatcat gggtcccgcc attcaaggtg
     4021 tgttgaccgg ttccaccacg gtggaagatt tcggcgccac gatctgtgac gaagccaaca
     4081 aagccttcaa tcaataagta acccctgtat gactggtggg gcaggcatgt actgcctgcc
     4141 ccactttgcc tgatgtacga agaaatcccc aaacttcaaa aagatttgca gcttcgagat
     4201 ttttttgtaa acgcactgac gccccatttc acggagatga gaatatgatg gctcaatcac
     4261 ggatagacgc tcagacgaat gtacctctgc gtattcaaac tctttggcgc ggattgttcg
     4321 ctttgctggc agttgcttcc attgggatga tatggacacg aacagtagag actccgctct
     4381 ggcaaaaaat agtcatttcg attgtagcgc tagcggcggc tgtgctcagt gggtatggtg
     4441 cttatcaaat ctcaaagcgc aatcacgccg ggcgtatgac ctcgctcgtg ttggattatc
     4501 tcggttttgt tgtctgcttt gtgtttgcct tgaatgtggg cgaattcttc gttggcatcg
     4561 atgccctcgg tgaaaatttt ggaaaaggca ttccctatct tggcatcacc gccctcggtt
     4621 attttctggg gatgttggaa gaatactttc caacaaagaa caagggctca gaaagcagcc
     4681 tcaaaactgt cagcaagtgg gtgatgttgg gcggtttcgc gcttttcctt tggcaggtca
     4741 atgcgctcaa tggcattatt tattttctcg gcaaacttgc ccaacccagc ggaatgatcg
     4801 cagttgttgg cttggtgatc tttgggctct cgctttggtc gatgaatcga ccagacatgg
     4861 cacatgcctt gaatgtcaag accgggcatg aacaaatcat caatggttgg ttgttccttt
     4921 cgccaaatct acttggcttc cttatcttct ttgcgggtcc gctgctgctt tcgttctatt
     4981 tttcctttac tgattcagac gctattcgca cgccgaattg ggtcggtttt gaaaactacg
     5041 ccaagatcct caacataaaa tttgtgaccc tcgactcgcc cgatcagctc gcccgtgatg
     5101 ctgtggatat caaagtctat gacgaagtgg ggcgtttcac ttatggagac acgggaatcc
     5161 tatttgctgc tgaagataaa ttcttctggc tcgcgcttcg caataccctg acctttgtgc
     5221 tgtttgccgt tcctttgagc gtgattcccg ctttggtctt atcgaatatt ctcaacagta
     5281 aattgcccgg tatgaagttc tatcgcgccg tgtacttcat gcccagcatc gcggcagtcg
     5341 ttggtatctc cctcgtttgg caatggttgt acaacgccac catcggttac atcaactact
     5401 tcatcacatt aggcatcgag ttttggaata acttatttaa tgtcgccatt atagatccta
     5461 aaatccaatg ggtctctgac gaaaagacag ccctctttgc catcatcatc atcgccgcct
     5521 ggcagacgat gggcttcaac accgtgc
//
LOCUS       DAOQNJ010000108         5536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8636_length_5536_cov_10.021529, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000108 DAOQNJ010000000
VERSION     DAOQNJ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8636_length_5536_cov_10.021529"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLG52_04615"
     CDS             complement(<1..296)
                     /locus_tag="PLG52_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB40759.1"
                     /translation="MAKPLALVIEDDPQQNQIFTLALKNSFEVEAISDGSQAISRLTK
                     VTPRLIVLDINLPGASGAKVLEVIRKDKRFSSTRVILATANSQEAEVLREKSDIV"
     gene            complement(356..5470)
                     /locus_tag="PLG52_04620"
     CDS             complement(356..5470)
                     /locus_tag="PLG52_04620"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB40760.1"
                     /translation="MNFIYTPYSIPLIAAAGISIFVAIYAWRHRSSPGAPALTLLAAA
                     IAQWILGYILEISSADLNTIYIWGVSQYIGIAFAPYAWLIFAIDFAGYSKLLTRRFYV
                     LTAIFPVITVLLAWTTRWHGLVWAKYDISLVEGFSALATQKGYWFYAHTAYSYLMLLI
                     GTILLVRALVRLQGLYRSQVIAMLIAVFAPWIGNVLYLTGNSPIPYLDLTPFSFTVTV
                     AALAWAIFGFHLVDITPIARDLVIDSLQEGVIVLDANDRVADINSSAARIFGIQISHA
                     IGTLASELFTPWQSLRERLREDEDWVQEFSNGVGQAERQYEAAISHIQDAQGRNAGRV
                     MIVREVLSSSTRRRPASSSTEAASVSDSVDTTIETPKWLDAYPWLKKIFVYFYTPINT
                     DLVVPPNISPTWYLARERSFTIILRVGALAGTIAYFFTLPVMRVASRFSISSLFFGIV
                     IVLLWFLGGGRKIGFETRANVFLVLIYFMSVNETIGYGFSVECFLFFTAFVTISTLLT
                     GRRGLRLATLTSFITLVLFAWLIGGGYYIPLNLTATTLRPQSLAIGFTNLFVFLATSS
                     AIANATIVLMENLNSAWRKESQTSNLLQQERDLLEQRVEERTHDLQEAESKFRTLVEQ
                     LPAVLYRDDADQNGKNNYYSPQAERMLGYPMSVWEKDSMLWHSILHPDDKERAIETIN
                     ETLSNGHSLSEYRLFDSDGRVVWVRDEAILVRESSGKPLFVQGIMQDITEIKKAEEEI
                     RKLSRAIEQSGNSIIITDTNGDIEYVNPTFEKITGYSFNEIKGQNPRILKSGRQRAEF
                     YDKLWATISSGNVWHGVFHNKRKDGTLYWENATIAPVMDATGRVTNYVAIKEDITARR
                     EADEQLRKLSQAVEQSANTVIIMDREGVIEYVNPTFSLITGYSSEDALGKTPERLLNP
                     ASKLDNFRNQEWWMTVNAGNTWRGEFRNYRKDGQVFWESASIAPVMDSNGAVTNFIEI
                     KQDVTEQRKLQDQLQKRNEYLSILHQVTLDLLNRRDVEDLLQVIVDRASVLLDATFSE
                     LMLEEDGYLVVKAFTANQPNLKGDRVGREQAKLSWQAFDTKEPVVLEDYATWEFRRDV
                     YDVNPIHATADFPVMAGETCIGVLALGRDQAGYAFTNEQIQTGILFARLVALVLDNVN
                     LYDSALREIAERERAQISLQRSNEQQQIIVSLLRVGLENMTLDQILGNILDGILSVGW
                     MKLVPKAGIFLVDKETQKLRLHTHRNFSPPLLNLCAQVDMGRCMCGRAAMQKQILFSD
                     CLDERHEIRYEGIEEHGHYNVPILQGKEVLGVIVLYLPHGYQRSTDDETFLLAIADAI
                     SGVIGRKRVEALLQGSEILFRQIVENASDIIYRTDMQGMFTYANPAALNMMGFTSEQE
                     VLGKNYLELTTPEARNRLKRVYDRQYLSRTNNTYYEFPAISMKGEIIWVGQNVQLIWD
                     GDKIVGFQALARNITQLRQAQEALLLARDQALEASRFKSQLVSRVSHELRTPLGGILG
                     FAELLKEGSFGSLNSSQMTAVSNIVESTNFLTDTVNDLLDQAQIEARSINLHNAYFKP
                     ADLMKKIAMTMSVLAGKKGLAFHTEVAADLPVELYGDEKRLQQIIVNLTGNAIKFTSH
                     GEVNIRFVMASPDRWALKVQDTGVGIPEDERNSIFEPFQQLSNSITRENRGSGLGLAI
                     VKQLAELMSGEISLQSEAGRGSTFTVTFPLLQPVEN"
BASE COUNT         1600 a         1345 c         1164 g         1427 t
ORIGIN      
        1 acaatatccg atttttcacg caaaacttca gcctcctgcg aattggcagt agcaagaatg
       61 acgcgcgtag agctgaaacg cttatctttt cgaatcacct caagtacttt tgcgccagaa
      121 gccccgggca aattgatatc caaaacgatt aacctcggcg tcactttggt aagacgagaa
      181 atagcttgag aaccatcact aatggcttca acttcaaatg agtttttcag agcaagagta
      241 aaaatctggt tctgttgtgg gtcatcttcg atgaccagtg caagtggctt tgccatatat
      301 ctcctaaaaa attagaattt tcaaaaatga tcactatacc tgatagtggt gtatctcagt
      361 tttcaacagg ctgtagcagc gggaaagtta cagtaaaggt acttccgcgc ccagcttcac
      421 tttgtaaaga gatctctccg ctcatcaact ctgccaattg tttaacaatg gcaagcccca
      481 acccagaccc gcggttctca cgtgtgatgg aattgcttaa ctgttgaaag ggttcgaaaa
      541 tactattacg ctcgtcttct ggaatgccca caccagtatc ctgcactttc aaagcccagc
      601 ggtctgggga tgccataaca aaacgaatat tcacttctcc atgagaagta aacttaatcg
      661 cattccctgt aaggttgaca ataatctgct gtaaacgctt ttcgtctcca tacaactcta
      721 caggcaaatc ggcagcaacc tctgtatgaa atgccaagcc cttcttacca gccaatacag
      781 acatggtcat tgcgatcttc ttcattaaat cagccggttt gaaataagcg ttgtgtaaat
      841 taatcgatct ggcttcgatc tgagcctggt ctaacaagtc attgactgta tctgttaaga
      901 agttggtgct ttcaacaata tttgaaacgg ctgtcatttg gctgctattg agagagccaa
      961 atgagccctc tttaagcagt tcggcaaaac ctaaaatacc tccaagaggg gttcgcagtt
     1021 catggctcac gcgtgagacc aattggctct tgaaacggct ggcttctaat gcctggtcac
     1081 gcgcgagaag caaggcttct tgagcctgcc tcaactgagt gatatttctc gccaatgcct
     1141 ggaagcccac aatcttatca ccatcccaaa ttaactgcac attctgcccc acccagataa
     1201 tttcaccctt catagatatg gcaggaaatt cataataggt attattcgtt ctgctgaggt
     1261 actgacggtc atacacacgc tttagacggt tgcgtgcttc tggagtggtt aattcaagat
     1321 aatttttacc aagcacttcc tgctcacttg tgaaccccat catgttaagg gctgccgggt
     1381 tggcatatgt aaacatccct tgcatatctg ttcggtagat aatatcactg gcattttcta
     1441 caatctgacg gaacaggatt tcgctccctt gcaacaaagc ttcaacacgc ttacgcccaa
     1501 tcacgccaga gatagcatct gcaattgcaa gcaaaaatgt ctcatcatcg gtactgcgtt
     1561 gatatccatg cggcaggtac aagacaatta cgcccaatac ttccttgccc tgcaaaatcg
     1621 gaacgttata atgcccgtgc tcctctatgc cttcataacg aatctcgtgc ctttcatcca
     1681 aacaatctga aaacaggatc tgtttctgca tggcagcacg cccacacatg cagcgaccca
     1741 tatccacctg agcacataaa ttcagcaaag gaggggaaaa gttgcgatgt gtgtgcaggc
     1801 gcaatttttg agtctctttg tcaacgagaa agatgcccgc tttcggaaca agtttcatcc
     1861 accctacaga aagaattcca tccagaatat ttcccagtat ttggtcaagg gtcatgtttt
     1921 caagcccaac gcgtaaaaga gatacgataa tctgctgttg ctcattggac cgttgcaggg
     1981 aaatttgagc acgttcacgc tcagcaattt ctcgcaaggc ggaatcatac aaattaacgt
     2041 tatctaacac aagggctaca aggcgggcga aaagaatgcc tgtttgaatt tgttcattcg
     2101 taaatgcata tccagcttgg tcacgcccca aggcgagaac gccgatacat gtctcaccag
     2161 ccataacggg gaaatctgct gtagcatgta tcgggtttac atcatatacg tcgcgccgaa
     2221 attcccaggt ggcataatct tcaagtacaa cgggttcctt tgtgtcaaaa gcctgccacg
     2281 aaagttttgc ctgctcacgc ccaacccggt cgccctttaa gtttggttgg ttggcggtaa
     2341 aagctttcac caccaaatac ccatcctctt ccagcatgag ctcgctaaac gttgcatcca
     2401 gcagaacaga tgcgcggtct acgatcactt ggagcagatc ttccacatca cgacggttca
     2461 aaagatccaa ggtaacttgg tgcaaaattg ataaatattc attccgcttc tgcaattggt
     2521 cttgcagctt tcgctgttca gtaacgtctt gtttgatctc aataaagttg gtcacagcgc
     2581 cattcgaatc catcacgggg gcaatactgg ctgattccca aaatacctgc ccatctttgc
     2641 gatagttgcg gaactcacct cgccaagtat tcccagcatt gaccgtcatc caccactctt
     2701 ggtttcggaa attatcaagt ttcgaggctg gattcagcag tctctcaggg gttttgccca
     2761 aggcatcttc agaagagtat cctgtgatca gtgagaaagt ggggttcaca tattctataa
     2821 caccttcacg gtccatgatg atgaccgtat tagcactctg ttctactgcc tgtgacaact
     2881 tgcgcaactg ctcatccgct tctcttcgag cagtaatatc ttctttaatt gccacataat
     2941 ttgtcaccct acccgttgca tccataacag gcgcaatggt ggcattctcc caatacaggg
     3001 ttccatcctt gcgtttgtta tgaaacactc catgccaaac atttccagag gaaattgttg
     3061 cccacaactt gtcataaaat tctgcacgtt gtctaccaga tttcaagatc ctggggtttt
     3121 gccccttgat ttcattaaaa gaatagcctg ttattttttc aaacgtgggg ttcacatatt
     3181 caatatcgcc attcgtatcg gtaatgatga tcgagtttcc gctttgctca atagcacgtg
     3241 ataattttcg aatctcttct tctgcctttt tgatctccgt aatatcctgc ataatgcctt
     3301 gaacaaacaa aggctttcca gacgattcac gcaccaagat ggcttcatca cgaacccaaa
     3361 caacccgacc atcactgtca aacagacgat actccgagag ggaatgtcca ttagagagcg
     3421 tctcattaat agtctcaatg gcacgttctt tatcgtcagg gtgaagaatg ctatgccaaa
     3481 gcatggaatc cttctcccaa acagacatgg ggtaccctag cattctctcg gcttgtgggc
     3541 tgtagtagtt gttctttccg ttttgatctg catcgtcgcg gtacaaaact gcaggtaact
     3601 gttcaacaag ggttcggaat ttagattctg cctcttgcaa atcatgtgtt cgttcttcca
     3661 cgcgctgctc aagaaggtca cgttcctgtt gcaataaatt tgaagtttgt gattccttac
     3721 gccaggcaga gtttaggttc tccatcaaga cgatggtggc gtttgcaatt gcagaagaag
     3781 ttgccaaaaa cacgaataaa ttcgtaaacc cgatggcaag cgattgcggg cggagtgttg
     3841 ttgccgttag attcagggga atatagtaac cgccaccaat aagccaggcg aataatacaa
     3901 gcgtgataaa actggtcagg gttgcaagcc gaagtccacg ccgcccggtt agaagagtag
     3961 aaatcgttac aaaagcagta aagaataaaa aacattccac tgagaaacca tacccaatgg
     4021 tttcattaac cgacataaaa tagattagaa ctaaaaagac atttgcacgt gtttcaaagc
     4081 cgatctttcg cccgccccca agaaaccata aaaggacaat aacaattcca aagaaaaggc
     4141 tggatattga aaaacgcgaa gcaacacgca tgacaggcag agtaaaaaag tatgcgatgg
     4201 tccctgccag tgcacctact cgcaagataa tggtaaaaga acgctcacgt gccaaatacc
     4261 atgtggggct aatattaggc ggcactacca agtctgtatt gataggcgta taaaaataga
     4321 caaaaatttt ctttagccat ggatatgcat ccaaccactt cggagtttca atcgtagtat
     4381 ccacgctatc agaaacagaa gcggcctcgg tggaggatga ggcgggtctt cgtcgcgtag
     4441 agctagaaag cacctcacgg acaatcatca ccctgccagc atttctcccc tgtgcatcct
     4501 ggatatgaga gatcgccgct tcatactgac gttcagcctg tccaactcca tttgaaaatt
     4561 cctgcaccca atcttcatct tcgcgcaaac gctcgcgaag tgactgccag ggtgtaaaca
     4621 actcagatgc cagtgttcca atggcgtgag aaatttggat accaaaaata cgagccgcag
     4681 acgagttaat atctgcaact ctatcattgg cgtccagtac aatcacacct tcttgaaggg
     4741 aatcgataac caaatctcta gcaatgggcg tgatatcaac aagatgaaat ccaaagattg
     4801 cccaagccaa tgccgcaacg gtgacagtga aagaaaaagg cgttagatcg agatatggaa
     4861 tagggctgtt gcccgttaga tacaaaacat tcccgatcca tggcgcaaag actgcaatta
     4921 gcatcgctat aacttggctt cgatacaaac cttgtaagcg gaccaaagcg cgcaccaaca
     4981 agattgttcc gatcagaagc atcaagtagg aataagcagt atgggcgtag aaccaataac
     5041 ctttttgagt ggcgagcgca gaaaaacctt caacaaggct aatgtcgtat ttcgcccaaa
     5101 ccaaaccatg ccagcgagtc gtccatgcca aaaggacggt gatgactggg aagatcgctg
     5161 ttagaacata aaaacgtcgt gtaagaagtt ttgaatagcc ggcaaaatcg atcgcaaata
     5221 ttagccatgc gtatggtgca aaggcaattc caatgtactg acttaccccc caaatgtaaa
     5281 tggtattcaa atctgcactg gatatttcca aaatatatcc aagaatccac tgagcaattg
     5341 cagctgcaag aagggtcaac gccggggctc cgggagaaga acgatgcctc caggcataga
     5401 tggctacaaa aatagaaatc ccagccgctg cgatcaaagg tatggaataa ggcgtgtaaa
     5461 tgaaattcat gggcgattcc tttggcaaaa tcagcccaat tatatagtca tcccctgctg
     5521 ttggttcatt acaaag
//
LOCUS       DAOQNJ010000109         5471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8791_length_5471_cov_8.323671, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000109 DAOQNJ010000000
VERSION     DAOQNJ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8791_length_5471_cov_8.323671"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5471
                     /locus_tag="PLG52_04625"
     CDS             <1..>5471
                     /locus_tag="PLG52_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB40761.1"
                     /translation="SGEMMLVEDMSVDPLYANTNWGGALIGLPLKFSNRVVGVMNIHF
                     AEPRIFTESELRLLKLFGDQAAVVIENSRLFNETERLLSETAGRNAELGVINSLQQVL
                     VSKLDFQSIIDLIGDKVGEIFDAQVTLISLYYPATGEMHHRYINERGQRMYFDEPMPV
                     DGFRKHVLESKQTLLISHDYLRMAEELGEEPASAGEAPKSLLFAPMVVDGQVTGIMSL
                     QNLDREHAFQESDTKLLSTIAASLSAALNNARLFEETMRHARESEALNEVGRDISATL
                     NLSAVLDKIAKHARDLLKGSSSAIYLPEPDGKSMRALVAQGDIAEEILADSIQVGEGI
                     IGSLAKLGKAEYINDTNHDPRTLQIPGTPAVQNERLLVTPLLSGEKVNGMMAVWRKGG
                     EPFSPADQRFQEELSLQAAIAIQNANFVNEVEQRAHELEIINSIQQGLSTKLDVQSVY
                     ELVGEKVRSIFNSQAVIISYYHPETETADFPYMYWKGERIYPGREPLSGFSGYVIRNK
                     APIFINSNLEEMAHSYGSTLLAGDTFPKSLIAIPILSGDQVFGSLSIQNFEQERAFTE
                     NDLRLLSTLAASMGISLQNAKLFDESQRLLVETRQRAEELALINTILSGLDTKLGIQT
                     VYDATGEKIREIFDAQTVVLSIYDKENQLTYYPYIIENGERLYQDPLPLREDGGGFGG
                     YVIRTKEPLLVNRDFEEYSKRFQSANLGVDAEVEVVVRAGLWVPMLVGDEVKGVISLQ
                     NLEREDAFSDSDVRLLSTIANSIGVAIENIRLFDETQRLLKETEQRATELAILNNVSE
                     SMSRALDVNAVTHTVGNKIHEIFSAEIVDILLYNSATNIVSLVFSFSNNLSYENEPPW
                     ELGEGLTSKIIITKQPLLLNTAKEIEDNGAATYVTAPEDEEDIKSYMGVPIMVGDQVL
                     GVVDVQSYKAHAFDEGSLRILQILSANMGVAIQNARLFDETQRLLKETEQQNSDLSAL
                     NSVQQALVSNIDVKSIYQAVGRKLTALFNVHSAVIYTVDLDTYSMTYEYAYEQGKEWD
                     IPPKQATSLHVYITDTVKTNRKSLVINKDFDEFAAQFPDFKTSRSRFPKSLCAVPILI
                     RKNSVTGISLQHLDTEDFFNDTSLRLLETIASATSVALENARLFDETQRLLNETKRRA
                     TELEILNNIGQVLTQQLDVNSIIEMVGDKVRELVNEDNLGIGLYDEETKTVTAHYVTK
                     GSERIQFPPFPINEFTQRAALQGKSLVINRRSPILWKKLGSHMTAMDEIPKSVVMVPM
                     IVGKELIGGFTIQNFEHENAYDESIITLLESIASGTATAIQNARLFEEARLARAAAEQ
                     ANEAKSAFLATMSHEIRTPMNAVIGMSGLLLDTELNDEQRDYVETIRGSSDTLLAIIN
                     DILDFSKIEAGRMDLEHQPFDLRECVEASLDLVTARAVEKSIDIAYIFEGDIPPAIVG
                     DVTRLRQVITNLLSNAVKFTDRGEVVLTVSSQRYKNGSAKEKVMLTFAVRDTGIGLTH
                     EGMSRLFQSFSQADSSTTRKYGGTGLGLAISKRLAEMMGGTMWAMSQGLGLGSTFMFN
                     IKAEVADLPPAKKQIHLGIQPALQNKRVLIVDDNATNRYILNMQTAKWGMVPRDTAFP
                     AEALQWIENGDFFDIAILDMHMPEMDGLELAKHLREKQMKFPLVLFSSLGRREAGDET
                     NLFSAYLSKPIKQSQLFDTLVGLFVQDEEENKKVTTDRFKPDPQMAARHPLRILLAED
                     NAVNQKLALRLLEQMGYQADVAVNGRETVQAVERKEYDVILMDVQMPEMDGLEATREI
                     VTRWAEKHPYIIGLTANAMQGDREMCLNAGMNHYIPKPIR"
BASE COUNT         1467 a         1349 c         1353 g         1302 t
ORIGIN      
        1 tctggcgaaa tgatgttggt agaagatatg agcgttgacc cactctacgc caacaccaac
       61 tggggtggcg cattgatcgg gttgccgctc aaattcagca accgtgttgt gggtgtgatg
      121 aatatccatt ttgcagaacc gcgtattttt acagagtctg aattacgcct actcaaattg
      181 ttcggagatc aagccgcagt cgtcatcgaa aattcgcgtt tgttcaatga gaccgagcgc
      241 ctcttatctg aaactgccgg gcgcaatgcc gagttaggcg tcatcaacag cctgcaacaa
      301 gtgctggtct ctaaactgga ttttcaatcc atcattgacc tgatcggtga taaggtcggc
      361 gagatctttg atgcgcaagt cacactcatc tcattgtatt atcccgccac aggtgagatg
      421 catcaccgct atatcaatga acgcggtcaa cgcatgtact ttgatgaacc catgcccgtg
      481 gatggtttcc gcaagcatgt gcttgaaagc aaacaaaccc tgcttattag ccacgattac
      541 ttaagaatgg cggaagaact tggtgaagag cctgcttctg caggtgaagc accaaagtcg
      601 ctgctctttg ctcccatggt agtggatggt caggtcacag gcattatgag cttgcaaaac
      661 ctggaccgtg aacacgcctt tcaagaatcg gatacaaagt tactttctac cattgccgct
      721 agcttaagtg cggcgttgaa taatgcccgc ttatttgaag aaaccatgcg gcatgcccgc
      781 gaatcagaag cactcaatga agttgggcga gatatatctg ccacgttaaa tctctcagcc
      841 gtgttagata agatcgccaa gcacgcccgc gacttattga aaggctcaag cagcgctatt
      901 tatttgcccg agccagatgg caagtcgatg cgggcgttgg tagcacaagg ggatattgca
      961 gaagaaattc tggcagattc cattcaggtt ggggaaggca ttattgggtc gcttgccaaa
     1021 ctcggtaagg cagaatacat caacgatacc aatcacgacc cgcgcacgtt acaaatccct
     1081 ggcacacctg cggttcaaaa tgaacgcttg ttggtcactc cgctgctttc tggtgagaag
     1141 gtcaacggta tgatggcggt ttggcgcaaa ggcggcgaac ctttctcccc tgcagaccag
     1201 cgttttcaag aagagttgtc tctgcaagct gcgattgcca ttcaaaatgc gaacttcgtc
     1261 aatgaagtgg aacaacgtgc ccacgaattg gagattatca atagcatcca acaagggtta
     1321 tcgaccaaat tagatgtaca gtcggtttat gaactggttg gagagaaagt tcgcagcatc
     1381 ttcaactcgc aagctgtgat catctcgtat tatcaccccg aaacagaaac tgccgatttc
     1441 ccttacatgt attggaaggg ggaacgcatc taccctgggc gtgagccgct ttctggtttc
     1501 tctggctatg tcattcgtaa caaagcgccc atctttatca actcgaactt ggaagaaatg
     1561 gcacacagtt acgggtctac tcttttggct ggggatacct ttccaaaatc gttgatcgct
     1621 attcccattc tctccggtga ccaggtcttc ggttcgcttt ccattcaaaa ctttgaacaa
     1681 gaacgcgcct ttacagaaaa tgacctgcgt ttgctttcga ccctggcagc aagcatggga
     1741 atttcgcttc agaatgccaa gctattcgat gagtcgcaaa gattgttggt cgaaacccgt
     1801 caacgcgcag aagaattggc gttgatcaac accatcttaa gcgggttgga taccaaactt
     1861 ggcattcaaa cggtatacga cgcaactggt gaaaaaatac gagagatctt cgatgcacag
     1921 accgttgtgc ttagcatcta cgataaagaa aatcaactca cctattatcc atacatcatc
     1981 gaaaacggtg aacggctcta tcaagacccc ttaccgcttc gcgaagatgg aggcgggttc
     2041 ggtggatacg tcatccgcac caaagagccg ctattggtca accgcgattt cgaagaatac
     2101 tccaaacgct tccaatcggc aaaccttggt gtggatgccg aagtagaagt agtggtgcgt
     2161 gccggactgt gggtgcctat gttggtcggc gacgaagtga aaggcgtgat ctcgctgcaa
     2221 aaccttgagc gagaagatgc ctttagcgac tcagatgtgc gcttgctttc aacgattgcc
     2281 aactcgatcg gggtggcgat tgagaacatt cgcctcttcg acgaaactca gcgcttgctc
     2341 aaagagaccg agcagcgcgc caccgaactt gccattctca acaatgtcag cgagtcgatg
     2401 tcgcgcgcgt tggatgtcaa cgccgtcact cacaccgtgg gcaacaagat ccacgagatc
     2461 ttcagtgccg agatcgtgga cattctgctt tataactcag ccacgaatat cgtgagtttg
     2521 gtattcagct ttagcaacaa tctttcttat gagaatgaac caccttggga attgggagaa
     2581 gggcttactt ccaagatcat tatcacaaaa cagccgttgt tgttaaacac tgccaaagaa
     2641 atcgaagaca acggtgcagc gacctatgtc accgcccctg aagatgaaga ggatattaaa
     2701 tcttatatgg gtgtgcccat tatggtgggc gaccaagtat taggcgttgt agatgtccaa
     2761 agctataaag ctcatgcctt cgacgaaggc agtttacgca ttctacaaat cctttctgcc
     2821 aatatgggcg ttgccattca aaatgcacgt ttattcgatg aaacccagcg cctcttaaaa
     2881 gaaaccgaac agcagaattc cgacctttcg gctctcaact cggtacagca agcattggtc
     2941 tccaacattg acgtgaagtc gatttatcaa gcggtagggc gaaaactaac cgcgctcttc
     3001 aacgtccatt cggcggttat ctatacggta gaccttgata cgtatagtat gacctatgaa
     3061 tatgcctacg aacaaggcaa ggaatgggac attccgccca aacaggctac cagtttgcat
     3121 gtgtacatca cagacactgt aaaaaccaat cgcaagagtc tggtgatcaa taaagacttt
     3181 gatgagtttg ccgctcaatt cccagatttc aaaacctcgc gcagccgttt ccccaagtcg
     3241 ctatgtgcgg taccgatctt gatccgcaaa aattcggtga cagggatttc cttgcagcat
     3301 cttgatacag aagatttctt caacgacact tcgctgcgtc tattagaaac cattgccagc
     3361 gccaccagtg tggcactcga aaatgcacgc ctctttgacg aaacccaacg cctcttaaac
     3421 gagaccaagc gccgtgccac cgagttggaa attctcaaca acatcgggca agtgctcacc
     3481 caacaacttg acgtcaacag cattattgaa atggtcggcg acaaagttcg ggaactcgtc
     3541 aatgaagata atttaggcat tggtttatat gacgaggaaa cgaagactgt cacagcccat
     3601 tacgtaacca aaggcagcga gcggattcaa ttcccgccgt tcccgatcaa cgagttcacc
     3661 caaagagccg cgttgcaagg caagagtctg gtcatcaacc gccgctcccc cattttgtgg
     3721 aagaagctcg gttcccatat gaccgccatg gatgaaatcc ccaaatcggt tgtaatggtg
     3781 ccaatgatcg tgggcaaaga actgatcggt ggcttcacca tccagaactt tgagcatgag
     3841 aatgcctacg acgaatccat cattaccttg ctcgagtcca ttgcttctgg tacagccaca
     3901 gccattcaaa atgcacgcct gttcgaagaa gcgcgtttgg cacgtgccgc ggcagaacaa
     3961 gccaacgaag ccaagagcgc cttcctcgcg accatgagcc atgaaattcg cacgcccatg
     4021 aatgccgtta tcggcatgag cggacttttg ctcgataccg aactcaacga cgaacagcgt
     4081 gattatgttg agaccattcg cggctcaagc gataccttgc ttgccatcat caacgacatt
     4141 ctcgacttct cgaagatcga agccgggcgc atggaccttg aacatcagcc ctttgacctg
     4201 cgtgaatgtg tcgaagcatc actagacctg gtcacggcgc gggctgtaga aaaaagcatc
     4261 gacattgcat atatcttcga aggcgatatt ccgcccgcta ttgtgggcga tgtgacgcgc
     4321 ttacggcagg tcatcaccaa tttgctcagt aacgcggtca agtttacaga cagaggtgag
     4381 gtcgttttaa cggtatccag ccaacggtac aagaatggaa gcgcgaaaga gaaagtcatg
     4441 ctcacgttcg cagtacgcga tacgggtatc ggtctcacac acgaaggcat gagtcggctc
     4501 ttccaatcgt tctcgcaagc tgattcatct accacgcgca agtatggcgg cacggggttg
     4561 gggcttgcca ttagcaaacg cttggctgag atgatgggcg gtacgatgtg ggcaatgagc
     4621 cagggcttgg ggttgggctc aacctttatg ttcaacatca aagccgaagt ggcagacctg
     4681 ccccccgcca agaaacaaat tcatcttggc attcaaccgg cattgcaaaa caaacgcgta
     4741 ttgatcgtgg atgacaatgc caccaatcgt tacattctca atatgcaaac tgccaagtgg
     4801 ggcatggttc cgcgtgatac tgccttccct gctgaagctt tgcagtggat cgaaaatggt
     4861 gattttttcg acatcgccat tctcgatatg cacatgcctg aaatggacgg cttggaattg
     4921 gcaaaacatt tgcgtgaaaa gcaaatgaaa ttcccgttgg tgttgtttag ttcgttgggc
     4981 aggcgtgaag ccggcgatga gacaaatctc ttctctgcct atctttcgaa acctatcaag
     5041 caatcgcaac tgttcgatac cttggtagga ctcttcgtgc aggatgaaga agagaataag
     5101 aaggtgacta cagaccgctt caaaccggac ccgcaaatgg cggcgcggca tccgttgaga
     5161 attctgctcg ctgaagacaa cgccgtcaat caaaagttgg cgttgcggtt gttggagcag
     5221 atggggtatc aggcagatgt ggcagtgaat ggacgtgaaa cggtgcaagc tgtggagcgt
     5281 aaagagtatg acgttatttt gatggatgtt caaatgccag aaatggacgg gttggaagca
     5341 acgcgtgaga tcgtgacacg ctgggcggag aaacatccct acattattgg gctgaccgcc
     5401 aatgccatgc aaggtgaccg tgagatgtgt ttgaatgctg ggatgaatca ttacattccc
     5461 aagcccatcc g
//
LOCUS       DAOQNJ010000110         5462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8822_length_5462_cov_9.642685, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000110 DAOQNJ010000000
VERSION     DAOQNJ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8822_length_5462_cov_9.642685"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..5340
                     /locus_tag="PLG52_04630"
     CDS             <1..5340
                     /locus_tag="PLG52_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB40762.1"
                     /translation="GVLREEAVRISQADCASAVLFELPSSQTEPVIQLAVGCDRVPTV
                     TPPEWTVIKSGEPLVISDYAREAILPPHENAVSAVILPVMFQTRVLGLLTVHSSRTGF
                     FTTETIELLQMLTSQAGIAVNNANRYQIEKQHSDLMRRRAETLVRLTDVSYNLGHDQP
                     LDQALQMIARAIRDATPFRVVLVSIFEAETSQLRRVTSVGLSQEAWNELSSRKQPLTS
                     VQQLMRSEFKISHSYFIPSDQTPIIPPDVHRVIVDASKTTTNNANTWQAEDFLLLPLE
                     NSEGQIVGLISVDDPSDGLRPDKATIEAVEVFAAQAALLISNTLRQTDLRSRIDSLSA
                     AFDRQQKLINMAKDDLPILLRKDLEQTISLHNLDRRAQRVRAGLAITESVSRQLDASS
                     ALSALGRETLTQLGMSVALVAENTPDGPRLLHVMGSLPRATNVEALFGQRNPMRSCLQ
                     NGMPILIPNLDEDDEWRDASLLTSLRAKGVICLPIIVEGKPVAAMLAASPEPMPSFTD
                     EDRHVYTQISQQTSVILQNISLLNQTRRRLDEVNLLLEFSRGLSGMDADSVVESLLAS
                     ARRVIPHAHAGVVLVWNSKTEHLVPRAVSGYADNDSMMKINYRLGEALPGTAFLNRKS
                     RRVDEINFPRDYNLNTENLAHYRQATGGRLPVSCLIVPIVSAEQNLGLLVLDNFNTTA
                     AFRPDDETLLVSLSQQVALSLDNMRLVQTSQERAGQLQALNDASASLTTSLSTDQLIQ
                     SLLDQLGPILPFDTATLWLRERDRLTVISTRGFSDVEERQGLSISVSDSALFKELAQT
                     GQPIFVKDVREDPRFPPVDAPRLSWLGIPLIYKNELMGVLAVEKWQANFYTREQMQAG
                     LTFASQAAVSLDNSRLFEDSVRRATELDQRSQRLTALNRFTSTLTRSLDTDQILTATA
                     EELFQGLGASRVSIVTFERGQNLWKYTLPRIRAKLPRILPDAPIFSRLRESQGIFNTD
                     DVRSEPDLVSILEMLGDQARALLILPLVSGGSLNALVFVQLMGETRFGINELEVARTI
                     TNQASIALENARLYQSSVRMADRFAVLNETSSLVSASLNPEEVYVSVHHAAERLMPLD
                     SFVITLFDETANEVDAVYLYDRGKRLPGEKVPFGKGMSSKVISSGKPFLIVDATKDTA
                     TDYVQAGDEADTTKSILAVPMILGGKALGMLSAQSYQTGVYSEEDVQLLGTLANQAIV
                     AIQNGRLFNETQKLAAELEMRVLDRTAQLQREKQNTDTLLRILTEVSSSLDLDRALNR
                     TLSLLNDASGAEQGTILLVHAEDNLLHYRAGYGYLSDRTSSSNKGFTLKIGEGLAGWV
                     VKHRQSVLINDLFEDPRWVRSTATGQDHRSAIAVPMSVGEDAIGVLMVFHRAKDFFSP
                     ELLNLVTAIAGQVAVAINNARLYELIRDQAERLGLMLRKEQVEASRSQAILEAVADGV
                     LVTGADNRVSFVNSSITRVLGVDDTQLLGKSLDGFTGLFGSSSTEWIKTIRRWSEDPS
                     TYKVGEMYAEQLELENGRIVLIHLAPVILIHDFLGTVSIFRDITREVEVDRLKSEFVA
                     TVSHELRTPMTAIKGYVDILTMGAAGALNENQMHFLEVVRNNIERLNILVSDLLDISR
                     IESGRVKLEQGKVNLYDIAEEVVADVLRRSQNESKPIALSLDAPKNLPPIQGDGARVR
                     QIISNLVNNAFNYTPENGAILVNIHDADGEVQVDVQDNGIGIKPEDQDRLFERFYRGE
                     HPLVLATPGTGLGLPIVRQLVEMHNGRIWMKSTGVPGEGSIFSFTLPVYKNGA"
BASE COUNT         1284 a         1348 c         1398 g         1432 t
ORIGIN      
        1 ggtgttttaa gagaagaagc tgttcgtatc tcccaagccg attgcgcttc ggcagttctc
       61 tttgaattgc ccagttctca aaccgagcca gtcatccaat tggcagtggg ttgtgaccgc
      121 gttccgacag tgactcctcc tgaatggact gtaatcaaga gcggtgagcc gcttgtgatt
      181 tcagactatg cccgagaagc cattttgccg ccgcatgaaa atgcggtgtc tgcggttatt
      241 ttgccagtga tgttccagac tcgcgtgctt gggttgctca cagttcactc aagtcgtaca
      301 ggcttcttca cgactgaaac tatagaactc ttgcaaatgc ttacttcgca ggcgggcatt
      361 gctgtcaata atgccaatcg ttatcagatt gaaaaacaac atagcgacct aatgcgccgc
      421 cgtgcagaga ccctcgtgcg cctgacggac gtcagctata acttggggca tgatcaaccg
      481 ttagatcagg cattgcaaat gattgcacgc gccatccgcg atgctactcc gttccgtgtg
      541 gtattggtca gcatctttga agcagaaacc agtcaattgc gccgtgtcac atctgtggga
      601 ctttcgcaag aagcatggaa cgaactttcc tctcgcaaac agcctcttac cagcgtgcag
      661 caattaatgc gctctgagtt caagattagc cactcttact ttattccgtc tgaccaaact
      721 ccgatcatcc cgccggatgt tcatcgtgtg attgtggacg cttcgaaaac gactacaaac
      781 aacgccaaca cctggcaggc tgaagatttc cttttgttgc cacttgaaaa ttcagagggg
      841 cagatcgttg ggcttatcag cgtggatgat ccttctgatg ggttgcgccc agataaggcg
      901 acgattgaag cggtggaagt cttcgctgca caagccgcat tgcttatcag caatacattg
      961 cgccagacgg atctacgttc ccgcatcgat tcgctttctg cggcatttga tcgccagcaa
     1021 aagctgatca atatggcgaa agatgatctg cccattttgc tacgcaaaga ccttgagcag
     1081 accatctcgc ttcataacct ggatcgacgt gctcaacgcg tccgcgcggg gttggcgatc
     1141 actgaatcag ttagccgcca attggatgca agctcggctc tttctgcgct cgggcgtgaa
     1201 actcttaccc aacttgggat gtctgttgcg ctggtcgctg aaaacacacc agacggtccg
     1261 cgtttgttgc atgtaatggg gagcctgcca cgtgctacca atgtggaagc attgttcggt
     1321 caacgcaacc cgatgcgctc ttgtttgcaa aatggtatgc ccattctgat tccgaacctt
     1381 gatgaggatg atgaatggcg cgatgcttca ttgcttacgt ctttgcgtgc caaaggcgtg
     1441 atctgtttgc ccatcatcgt ggaaggcaag ccagtggctg ccatgttggc ggcaagccca
     1501 gagccaatgc catccttcac tgatgaagac cgccacgtat acacccagat ctctcagcag
     1561 acttcggtca ttctgcaaaa catttcgctc ttgaaccaaa cccgccgcag gttagacgaa
     1621 gtgaacctgt tgcttgaatt cagccgtggc ttatctggca tggatgcaga ctcagtggtt
     1681 gagtctctgt tggcgagtgc ccgtcgggtg attccgcatg cacatgccgg tgttgttttg
     1741 gtttggaata gcaagacaga gcaccttgtc cctcgtgcgg tttctggtta tgccgataac
     1801 gatagcatga tgaagatcaa ttatcgtctc ggtgaagcct tgcccggtac agcattcttg
     1861 aatcgaaaat cgcgccgtgt ggatgagatc aacttcccgc gtgattacaa cctcaatact
     1921 gagaaccttg ctcattaccg tcaggcaacg ggcgggcgtt taccagtatc ttgtttgatc
     1981 gtgccgattg tttcagcaga acaaaatctg ggtctgctcg tactcgataa cttcaacacc
     2041 actgcagcct tccgccctga tgatgaaacc ttgttggtct cactttcaca acaagtggcg
     2101 ctctcgcttg acaatatgcg cttggtacaa acctctcaag agcgcgcagg tcaattgcag
     2161 gcgttgaacg atgcgtcggc ttcgttgacg accagcttga gcacggatca gttgattcaa
     2221 tctctgctcg atcagcttgg accgatctta cctttcgata cggcgacgct ctggcttcga
     2281 gaacgcgacc gcctaaccgt gatttctact cgcggtttct cggatgtaga agaacgtcaa
     2341 ggcctgtcca tctctgtgag tgacagcgcc ttgttcaaag aattagcgca gactggtcag
     2401 ccaatcttcg tcaaagatgt gcgtgaagac ccgcgcttcc cacctgtgga tgcaccacgc
     2461 ctttcctggt tgggcattcc gcttatttat aaaaatgaat tgatgggtgt gcttgctgtt
     2521 gaaaagtggc aggcgaattt ctatacccgc gaacagatgc aagccgggtt gacctttgct
     2581 tctcaagcgg cggtctctct tgataactca cgtctctttg aagacagcgt gagacgtgct
     2641 acggaattgg atcaacgttc tcaacgtctt accgcgttga accgctttac atctacactg
     2701 acacgctcgc ttgatactga tcaaattctt acagcgactg cggaagagtt gttccaaggc
     2761 ttgggtgcca gccgtgtctc tatcgtgacc tttgaacgcg gacaaaactt gtggaaatac
     2821 accctgccgc gtattcgtgc caagcttccg cgcatattgc ctgatgcgcc gatctttagc
     2881 cgcttgcgcg aatcacaggg tattttcaat actgatgatg tccgcagtga gccggatctt
     2941 gtatccattc ttgaaatgtt aggagaccag gctcgggcgt tgttgatttt gcctttggta
     3001 agcggcggtt cgctaaatgc attggtcttt gtgcaactca tgggcgaaac ccgttttggt
     3061 atcaatgagt tggaagttgc gcgcacgatc actaatcagg caagcattgc gttggaaaat
     3121 gctcgcttgt atcaatcgtc tgttcgcatg gcagaccggt ttgcggtgct caatgagacc
     3181 agttccttgg tcagcgccag cctcaaccct gaagaagtat atgtttccgt ccatcatgca
     3241 gcggaacgtc tcatgccgct tgactcgttt gtgatcacct tgtttgatga aactgcaaac
     3301 gaggtagatg ctgtttatct ttatgatcgc ggcaagcgtt tgcctggaga aaaggttccg
     3361 ttcggtaaag ggatgagcag taaagtcatc agctctggca agcccttctt gatcgtagat
     3421 gctacgaaag ataccgccac tgattacgtc caagctgggg atgaggcgga tactaccaag
     3481 tctattcttg ctgtgccaat gatcttgggt ggtaaagcct tgggtatgct ttctgctcaa
     3541 agctaccaaa cgggtgtgta ctcagaagaa gatgtgcagt tgcttggaac cttggcgaac
     3601 caagctattg ttgctatcca gaacggacgt ctcttcaacg agacgcagaa attggcggct
     3661 gaacttgaaa tgcgcgtgtt agaccgcaca gctcaattgc agcgcgaaaa acaaaacacg
     3721 gatacgctct tacgcatttt gactgaggta tcttccagcc tcgaccttga ccgtgctttg
     3781 aatcgcacgc tctcgttgtt aaatgacgct tctggcgcag aacaaggcac gattttgctc
     3841 gtccatgcag aagataattt gctgcattat cgcgcagggt atggatattt gtctgaccgt
     3901 acttcttcgt cgaataaggg ctttacctta aagatcggtg aaggtttggc gggttgggtc
     3961 gtcaaacatc gtcagtcggt gcttatcaat gatttgttcg aagacccgcg ttgggtacgc
     4021 agcacagcga caggtcaaga tcaccgaagc gcaattgctg tacccatgtc ggtgggtgag
     4081 gatgccatcg gtgttttgat ggtcttccat cgcgccaaag atttcttcag ccctgaattg
     4141 ctcaacctgg tgactgcgat tgcaggtcag gttgcggtgg cgatcaacaa tgcccgctta
     4201 tatgagttga tccgcgatca ggcagagcgc ctcgggttga tgttgcgcaa ggaacaagtg
     4261 gaagcgagcc gctcgcaagc catccttgaa gcggtggcag atggagtgtt ggtaacaggc
     4321 gcagataatc gcgtctcatt cgtgaactca tccattactc gcgtgcttgg cgttgacgat
     4381 acccaattgc ttggtaaatc gcttgatggg tttactggtt tgttcggtag ctcttccacc
     4441 gaatggatta agaccattcg ccgttggtct gaagacccat ccacatacaa agtgggtgag
     4501 atgtatgcag aacaattgga attggaaaac ggacgcattg tcctcatcca cttggcgccg
     4561 gtgatcctca tccacgactt cctcggcacc gtatccatct tccgtgatat tacccgtgaa
     4621 gtggaagtgg atcgcctcaa atctgaattt gtggcgacgg tcagccatga gttgcgcaca
     4681 cccatgaccg ccatcaaagg ttatgtagat attctcacca tgggtgcggc gggtgctttg
     4741 aatgaaaacc agatgcactt cctcgaagtg gttagaaata acattgaaag acttaacatt
     4801 ctcgtgagcg atttgcttga tatttcccgc atcgaatctg gacgtgtgaa acttgagcaa
     4861 ggcaaggtca atctgtatga tattgctgaa gaagtggtgg cagatgtttt acgccgctca
     4921 caaaatgaaa gcaagccgat tgcactttca ctagatgcgc cgaagaacct tccaccgatt
     4981 caaggcgatg gcgcaagagt ccgccagatc attagcaacc ttgtaaacaa tgcattcaat
     5041 tacacccccg aaaatggcgc cattctggtg aatatccatg atgctgatgg cgaagtgcaa
     5101 gtagacgtgc aggataacgg cattggcata aagcctgaag accaagaccg cctctttgaa
     5161 cgtttttacc gtggcgagca tccacttgtg ttggcaacgc cgggcactgg tttaggtttg
     5221 cccatcgttc gtcagttggt cgaaatgcac aatggacgta tttggatgaa aagcacaggt
     5281 gtccctggtg aaggaagtat tttctctttc accttgcctg tctataaaaa cggagcataa
     5341 atttcaatgg caagaatttt gatcgcagaa gatgaaccgg atattcgtga gttggtggcg
     5401 tttacattac gctttgcggg tcatgaagtg accaccactg ccaacggcga agaagcctat
     5461 ca
//
LOCUS       DAOQNJ010000111         5442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8864_length_5442_cov_8.185075, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000111 DAOQNJ010000000
VERSION     DAOQNJ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8864_length_5442_cov_8.185075"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PLG52_04635"
     CDS             <1..1062
                     /locus_tag="PLG52_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40763.1"
                     /translation="LVKIAGRWFPRALLVDINEGQLNLVEAVLDMAGGEPLTAADLMK
                     DTELPTGVNPKLVEFSLNYAMQYDDRFDEVGPAGQVLWCLRRLEPDFVRDVPPPLRYM
                     DIPHDRSFLSPEMLALEAQLDDELTETDAADRDDVSSVTITLIYPHLRAGTLPVSPRT
                     RKLFPTAYETPRVRFTLVDGKTKQRIPAWVVLEHGYVFGLREWYKAHQLIPGSLVQVR
                     KGENPGEVIVEAKSQRSAKDWVRTVMVGSDGGFVFATLKQPITAEFNDRMVVHVPDFK
                     ALDPIWEKKRSFEDLLLMVMRELAKSNPQGHVHAQELYAGVNLVRRVPPAPIFAALAS
                     NPVFKHVGDLHFRLDEEVN"
     gene            1062..1496
                     /locus_tag="PLG52_04640"
     CDS             1062..1496
                     /locus_tag="PLG52_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40764.1"
                     /translation="MSLLKPTVKTPFHIDFEWWKQNEGDWHIFLRSFLCAEHQEAFAN
                     VEEGEMIDWIDPQTAEVKPVDGVQHALISHCAQLPDFVNQRTAIVEAIFRLFLANGNI
                     PMSAEDLSKKLGKPADTILRTIAGPRVYRGLRPMLNQPTETM"
     gene            1521..1593
                     /locus_tag="PLG52_04645"
     tRNA            1521..1593
                     /locus_tag="PLG52_04645"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1554..1556,aa:Arg,seq:tct)
     gene            1628..2011
                     /locus_tag="PLG52_04650"
     CDS             1628..2011
                     /locus_tag="PLG52_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNB40765.1"
                     /translation="MPTVIRKTRETLMEVRRGVLHAVSWQVKSSVWDPPTDLYETEEA
                     YIVRMEIAGMREENFEVSLLNDTLYITGFRHDTPEKRAVHQMEIRSGKFASMVRLPEP
                     VNVEKSLAEYQDGFLTIILPKETNE"
     gene            2024..4639
                     /gene="lon"
                     /locus_tag="PLG52_04655"
     CDS             2024..4639
                     /gene="lon"
                     /locus_tag="PLG52_04655"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNB40766.1"
                     /translation="MPAQKWQSGLQNFLQMMGENEDEFSNQFMNSFFSQLQKKDDDSN
                     SSQSNGDNEKMSLPDVLPILPLRGVVVYPNTAVPLTVGQARSIKLVDDVVSGDKLVGL
                     VAALNPEQEMPGPNELYRVGTVATVHRLLRAPDGTVRLLVQGMERFRLGEFVEEEPYL
                     KAKIIRAPELDEKNIETDALARNARDQFQAITQMIPSFPEELSSSILSLEDPLQTVYT
                     IANFQRIDLKDAQEILEIDSVYDKLKKLIGLLVREAEVLQIGQKIQNEARGEIEKVQR
                     DYFLREQMKAIQRELGERDEQSEEVDEFRKKIDEAKMPEEADKQARRELDRLSRLPTA
                     AAEYGVIRTYLDTMVSLPWSAGTEDNLDIAHARQVLNQDHYGLEDVKDRILEFLAIRK
                     LRLDRANDKKEESKDQIRHEREGVILCFVGPPGVGKTSLGQSIARAMGRKFIRASLGG
                     VRDEAEIRGHRRTYIGAMPGRILQSLRRVGSKNPVFMLDEVDKLTNDFHGDPSSALLE
                     VLDPEQNGEFRDNYLEVAYDLSQVFFIATANTLEYIPGPLRDRMEIIYLSGYTETEKT
                     QIARGYLIPRQIRENSLRKSEIAFNDEALKLIIRQYTREAGVRNLERKIGAVCRKVGT
                     RIAEGKKKKYNINPKVVEEFLDNPIFLGPEELNKRTSIPGVVPGLAWTSFGGDILLIE
                     ATSMPGGRGFQITGSIGNVMQESARAALSYVRSRSNSFGIAHEYFEKHDIHMHVPAGA
                     QPKDGPSAGVTMATSLVSLITGRKVSPQVGMTGEITLRGQVLPIGGVKEKVLAAHRSG
                     LGTVILPKQNEKDLDDVPDEIRKSMKFVFAETVEDVINSALEKPKQKKKTTKKETKPS
                     KKKVTQNAKSTPRRR"
     gene            4611..4967
                     /locus_tag="PLG52_04660"
     CDS             4611..4967
                     /locus_tag="PLG52_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB40767.1"
                     /translation="MPKVLLAEDDFTMVSLLKTLLKMEGYEVIALDADADVIAAVRSM
                     KPDVLLLDVHLFSQSGLDILDTLRRSKDTSKVRVLMSSGSNVRDECMMRGANGFLMKP
                     YMPDDLFTQLKQVLSV"
     gene            4964..5395
                     /locus_tag="PLG52_04665"
     CDS             4964..5395
                     /locus_tag="PLG52_04665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HNB40768.1"
                     /translation="MTQKFQIREFIFDQDYEPVLRLWQNIESGMQVGRSDSPEEIKRK
                     VERDPDLFLLAEIEGTIIGTVIGGFDGRRGMVYHLAVAQKHRRQGVAAALLAEVEKRL
                     QAKGCVKVYLLVLEDNTSAIRLYEECGWKHQKQDLIFSKEF"
BASE COUNT         1412 a         1243 c         1374 g         1413 t
ORIGIN      
        1 ttggtgaaaa ttgcaggacg ttggttccca cgcgctttgt tagtggatat caacgaaggt
       61 caattaaatc ttgtagaagc tgttttagat atggcaggtg gtgaaccact aaccgccgcc
      121 gatttgatga aagatactga gctgccaact ggcgtaaacc ccaagctagt tgagttttct
      181 ttgaactatg cgatgcagta cgatgatcgc tttgacgaag tgggtcccgc cgggcaggta
      241 ctttggtgct tacgccgcct cgaaccggac tttgtgcggg atgtccctcc gccgcttcgc
      301 tacatggaca tccctcatga ccgcagcttc ttatctcctg aaatgctcgc gttggaagct
      361 caactcgacg atgagctaac tgagacagat gctgcagatc gcgatgacgt ttcttccgtt
      421 accatcacct taatctaccc acaccttcgt gccgggacgt tacccgtctc accgcggacc
      481 cgcaaactgt tcccaactgc gtatgagacg ccacgtgtgc gttttaccct tgtagacggt
      541 aaaaccaaac aacgcattcc ggcttgggtg gttttggaac acggctatgt ctttggttta
      601 cgtgaatggt acaaggcgca tcaattaatc cctggttcgt tggttcaggt gcgcaagggt
      661 gagaaccccg gtgaagtgat cgtggaagca aaaagccagc gttctgcaaa ggattgggtc
      721 cgtaccgtaa tggttggttc agatggcggc tttgtctttg caacccttaa acagcccatc
      781 actgcggaat ttaatgaccg tatggtggtg catgtccctg atttcaaggc gctcgacccg
      841 atctgggaaa agaagcgctc ttttgaggat ttactcttaa tggtcatgcg tgaattagct
      901 aaatcaaacc cgcaaggaca cgtccatgcg caggaattgt atgctggcgt gaatcttgtt
      961 cgccgtgtgc cgcctgcacc gatctttgct gctttggcat cgaaccccgt tttcaagcat
     1021 gttggcgatt tgcacttccg cctcgatgaa gaggtgaact aatgagtctc ttaaagccga
     1081 cagtaaaaac accgtttcat attgattttg aatggtggaa gcaaaatgaa ggggactggc
     1141 atattttctt gcgctccttc ctttgtgccg agcatcagga agcgtttgcc aatgtggaag
     1201 aaggcgaaat gatcgactgg atcgacccgc aaactgccga ggttaaaccg gtggatgggg
     1261 ttcagcacgc tttgatcagt cactgtgctc agttgcccga tttcgtcaat cagcggacgg
     1321 cgattgttga ggcaattttc cgcttgttcc ttgctaacgg aaatattccc atgtctgctg
     1381 aagatttaag caagaaattg gggaagcctg ccgacaccat cctgagaact attgcgggtc
     1441 cgcgggtgta ccgtggttta cgtcccatgt tgaaccagcc cactgagacc atgtagtcgg
     1501 gtaaaattaa agcaacacaa gccctcgtag ctcaatggac agagcagcag ccttctaagc
     1561 tgttggttgg gagttcgatt ctctccgggg gcgctcgaaa gctggcaaaa tattaaggag
     1621 tcgtcctatg cctactgtga ttcgaaaaac acgcgaaaca cttatggagg tcaggcgcgg
     1681 cgtcctgcat gcagtgagtt ggcaggtcaa atcaagcgtc tgggatcctc caactgacct
     1741 ctatgagacc gaagaagcct atattgtgcg catggaaatt gcaggaatgc gcgaagagaa
     1801 cttcgaggtt tcactgttga acgatacgct ttacatcact ggttttcggc acgacacccc
     1861 agaaaaacgc gcagttcatc aaatggaaat ccgctctgga aagtttgctt caatggtgcg
     1921 gcttcctgag ccagtcaatg ttgaaaagtc actggcagaa tatcaagatg gttttctaac
     1981 catcatcctc ccaaaagaaa cgaacgaata aggttattac tcaatgcctg ctcaaaaatg
     2041 gcaatctggc ttgcaaaatt ttcttcaaat gatgggcgaa aacgaagatg agttttcgaa
     2101 ccagttcatg aactctttct tttcacagct tcagaagaaa gatgatgact caaatagctc
     2161 gcaatcgaat ggcgacaatg aaaagatgag tcttcctgat gtgttgccca tcctgccttt
     2221 gcgtggtgta gtggtatatc caaataccgc cgtcccgttg acggtggggc aggctcgttc
     2281 cataaaatta gtggatgacg ttgtctctgg cgataagctt gtcggtttgg tggcggctct
     2341 taacccggaa caggaaatgc ccggacccaa tgaactatat cgagtcggga cggttgctac
     2401 ggtccatcgt ttgctgcgcg caccagatgg aactgtccgc ttgttggtac aaggcatgga
     2461 gcgttttcgt ctgggcgaat ttgtcgaaga agagccttat ctcaaggcga agattattcg
     2521 tgcaccagag cttgatgaaa aaaatatcga aacagatgcc ttggctcgca atgcacgtga
     2581 ccaattccaa gcaattactc aaatgattcc ctcttttcca gaggaattat caagttctat
     2641 cctttcattg gaagatccgc tccaaactgt ttacaccatt gcgaactttc aacgcattga
     2701 cttgaaagac gctcaagaga ttcttgaaat cgactcagtc tacgataagc tcaagaaact
     2761 gatcggcttg ttggtgagag aagccgaggt tttgcagatc gggcaaaaga tccagaatga
     2821 ggcgcgtgga gagattgaaa aagttcaacg cgactacttc ctccgcgagc agatgaaagc
     2881 gattcaacgg gaactcggcg agcgtgacga gcaatcggaa gaagttgatg aattccggaa
     2941 aaagatcgac gaggcgaaga tgcctgaaga ggctgataaa caagcccgac gtgagttgga
     3001 ccggctctct cgtcttccta ctgctgccgc agaatatggt gttattcgca cctatttgga
     3061 cacgatggtt tcgttgcctt ggtctgcagg tacagaagac aatcttgaca tagcccatgc
     3121 tcgccaagta ttgaaccagg accattatgg tttggaagat gtcaaggacc gtattcttga
     3181 attccttgcc attcgtaaat tgcgattgga tcgtgccaac gataaaaaag aagaatcgaa
     3241 agaccagatt cgccatgaac gcgaaggtgt gattttgtgt tttgtgggtc ctcctggggt
     3301 tggcaaaact tctttgggac aatctattgc ccgtgcaatg gggcggaagt tcattcgcgc
     3361 atcattaggt ggtgtgcgtg atgaagccga gattcgtggg catcgacgta cctatatcgg
     3421 tgccatgccg ggtcgtattt tgcagtcgct gaggcgggtg ggttccaaga acccggtctt
     3481 tatgcttgat gaagtcgata aactcacgaa tgactttcac ggcgacccat cctctgcctt
     3541 gctggaggtg ctcgaccctg aacagaatgg tgagttccgc gataattatc ttgaagtagc
     3601 atatgacctt tcgcaggtgt tctttattgc cactgcaaat acgttggaat atatccccgg
     3661 tccgctgcgt gaccgcatgg agattattta tctttcaggt tataccgaaa ctgagaagac
     3721 tcaaattgca cgcggctatc tcattccgcg ccagattcgt gagaatagtt tacgtaaatc
     3781 tgaaattgct ttcaatgacg aagccttgaa gttgattatc cgccaatata cgcgtgaagc
     3841 tggggttcgt aatttagaac gcaaaatcgg cgctgtttgt cgtaaggttg gtactcgcat
     3901 tgcagaaggc aagaaaaaga aatacaacat caacccaaaa gtagtggaag aatttctcga
     3961 taaccccatc ttcctcggac ctgaagagtt gaataaacgc acttcgattc ccggtgtagt
     4021 gcccggtttg gcgtggacct cttttggggg tgacattctt ttgattgaag ccacttccat
     4081 gccaggaggt cgcggtttcc agattacagg ctcgattgga aatgttatgc aggaatctgc
     4141 gcgggcggct ctttcatacg tccgctcgcg ttcgaactct tttgggatcg cgcatgaata
     4201 ttttgagaag catgatattc atatgcacgt cccagcagga gctcaaccca aagacggtcc
     4261 ctccgctggt gtgacgatgg caacctcact cgtgtcactc attactgggc gaaaggtcag
     4321 cccgcaggtt ggaatgaccg gtgagattac cttgcgaggt caagtcttgc ccattggcgg
     4381 cgtcaaagaa aaggtgcttg ctgctcaccg tagcggactt ggcaccgtca tcttgccaaa
     4441 acagaatgaa aaagatctag acgatgtgcc agacgagatt cgtaaatcta tgaaatttgt
     4501 ttttgctgaa accgtcgaag atgtcatcaa ttcggcgttg gaaaaaccga aacagaaaaa
     4561 gaagacgact aagaaagaaa caaaaccttc gaaaaagaaa gtaacccaga atgccaaaag
     4621 tactcctcgc cgaagatgat ttcaccatgg tttccttgct caaaactctc ctgaaaatgg
     4681 agggctatga ggttattgcc cttgatgcgg atgcggatgt gattgcagcc gtccgttcta
     4741 tgaaaccgga cgttctcctg ttagatgtcc atttgttcag ccagagcggg ttagatatcc
     4801 ttgatacctt gcgtcgctca aaagatacta gcaaagttcg tgtgttgatg tcttctggtt
     4861 caaacgtccg tgatgaatgt atgatgcgcg gcgcgaatgg ttttcttatg aaaccctata
     4921 tgccagatga cctcttcaca cagctcaaac aagtcttatc ggtatgaccc aaaagtttca
     4981 gatacgagag ttcatcttcg atcaagatta tgagcccgta ttgaggcttt ggcaaaatat
     5041 cgagtctggc atgcaagttg gtcgttctga ctctccagaa gaaatcaaac gtaaagtaga
     5101 gcgcgaccct gatttgtttt tgttggctga gatagaaggc accatcatcg gaacagtcat
     5161 tggcggtttc gacggcagac gcggcatggt ttatcacctt gcagttgctc aaaaacatcg
     5221 caggcaaggt gtggcagctg ctcttcttgc cgaagttgaa aaacgtttgc aagccaaagg
     5281 ctgcgtcaag gtctatctac tagttcttga agacaataca tctgcgattc gcctttacga
     5341 agaatgtggc tggaaacatc aaaaacaaga ccttattttt tctaaagagt tttaaatgac
     5401 aatgcgattt ggaatcatca ccctctcaga tcgttcttca cg
//
LOCUS       DAOQNJ010000112         5425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8910_length_5425_cov_9.394972, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000112 DAOQNJ010000000
VERSION     DAOQNJ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8910_length_5425_cov_9.394972"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLG52_04670"
     CDS             <1..322
                     /locus_tag="PLG52_04670"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNB40769.1"
                     /translation="CSSCDTSIEGSFALGQFSNLSPEQMEFVFTFVRCEGKINRMEQE
                     LGLSYPTIRNRLHEVIRKLGFEPGRDETPEVDDETRSSVLDDLESGKITADQAMRILR
                     GEEE"
     gene            326..1423
                     /locus_tag="PLG52_04675"
     CDS             326..1423
                     /locus_tag="PLG52_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40770.1"
                     /translation="MSKTISAGRTPRIEITAVSGDLSLVGWEGDDILLKADDEEMNVK
                     QDGDQVTIACKDDLSMRVPKGSTVIIHRITGDASVRGVMGAIELKEISGDLSMRDVVT
                     VAIDTVHSDFSLRGAKGSLSIRKAGSDVSVRDVDGNVAIESVSDDLALRDVRGSVTAN
                     VGEDVVMYLNPQAGNTYAVNAGDDILLVMPPKANATLTLSADEIDVDWDGVENEEDAT
                     SRVIILGDGSANLTLSAGGDIRVSNRSDAGESAEDFGNFAGMGMDWSGFGERISRRVE
                     QATSRAQRKAEEAARRAENKMRDAERRGRGKVAFGVGRWNWDFSPKGVPVPPKQQVSD
                     EERLAILKMLQEKKITAEEAEKLLAALEGGS"
     gene            1423..2085
                     /locus_tag="PLG52_04680"
     CDS             1423..2085
                     /locus_tag="PLG52_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40771.1"
                     /translation="MSSEERRKILQMVADGKISAEEAATLMRALDDSNAEEASAEMEI
                     IEVRPASSAEGAGSSYQEKSHAPELDAVRMRAHRFSGAFLWIGTIVTVLIAWWMFNVQ
                     QNSGLNFWFFCLSLPLTLGILLIALGAGSRTSRWLYVNVDRSQSKDQDGPRKISLAFP
                     LPLGFASWFLRTVGRHIEELRDKNVDAIADTIALAKNMTEPLIVHVDDDSDGTQVQVF
                     IG"
     gene            2091..3383
                     /locus_tag="PLG52_04685"
     CDS             2091..3383
                     /locus_tag="PLG52_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40772.1"
                     /translation="METIKKQPLLSGILILFLTAMIFANIGGNMYEGLMPLYLKELKA
                     DITDIGLFFTLAQIVPLFLQILGGWVSDSLGRLRAIAIGSVFGLLGFIPLVLADTWHW
                     LLIATAVGAIARALVGPSFDAFIAEHSTEENRGKVYGISQAIFMIVTVVGPPLGGWLT
                     GAYGFKLMLFIAGVFYAIAVAMRLSMAREATKGEKENGEKKSLSFTSLKENLGSMIAL
                     IFSGGLITWMLITDGLRDTSFQFSGNLFPVYMQEIGGLSYQQIGWVTSMFGIFMMLST
                     IPGGWLSDKAGERVGIAAGMFLMSVALFILIVIPQGTQWQWLFFVGWAVAGLGAGISG
                     PAYQSLISKAVPQKLRGLAFGLFSTSLGIVSLPAPWIGAQLWARFGPTAPFKITATVI
                     LLSIIPIWLKFKLPKTTEETQPEAEQPVEENKVAIAAG"
     gene            3424..3810
                     /locus_tag="PLG52_04690"
     CDS             3424..3810
                     /locus_tag="PLG52_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40773.1"
                     /translation="MANVEERMKILKLIEEGKISAEEGAKLLSALNDSRKNIPTPPRP
                     PSAPGGARMLRVRVTDTRTGRSKASVQIPLALVDAGLKIGAHFAPEVQGVDMSNVMEA
                     LRMGVTGKIIDVTDEEDGEHVEIFVE"
     gene            3962..5305
                     /locus_tag="PLG52_04695"
     CDS             3962..5305
                     /locus_tag="PLG52_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40774.1"
                     /translation="MQETKTNRPSGMFAFTIVWLGQIISVLASAMSQFGLTIWMYEKT
                     QSATALGLMQVFFITPFLIISPIAGVMVDRHNRKLMMMVSDLTAGLATILILIFQSLG
                     VLQFWHLYVASIIYGLGNAFQWPAYSAAISTMVPKDQYGRANGMMSLIEAGPGVFAPL
                     LAGALLPLIGLTGILVIDVVTFSFAVGALLIVHIPQPERTVEGVQGKGSVWQEAVFGF
                     KYIFARPSLLGLQMVFFFGNLFSGILFTLLAPMILARTDNNSLMFGTVQTAGAIAGVV
                     GGLIMSAWGGFKRKVHGVLGGWAITGLGSAVIGLSFGLPAWVIGVAIASLISPLVNAS
                     NQAIWQSKVAPDLQGRVFSARRLIAWFTNPISPIVAGTLADYVLEPQMRAGGTLSQTF
                     SWLVGTGPGSGMGLIILACGVFCVLIGVAGYFIPAIYNAETILPDHDQLAKVEPA"
BASE COUNT         1251 a         1116 c         1507 g         1551 t
ORIGIN      
        1 ctgctccagt tgcgatacct caattgaagg tagctttgcg ctggggcagt tttcgaatct
       61 ctctcctgaa cagatggaat ttgttttcac ctttgttcgt tgcgaaggca agattaatcg
      121 catggagcag gaattagggc tttcctaccc aaccattcgc aaccgcttgc acgaagtcat
      181 tcgcaaactt ggttttgaac ctggtcgaga tgagacccct gaagtggatg atgaaacccg
      241 cagcagtgtt ctcgatgatc tcgaatccgg aaagatcaca gcagatcaag ccatgcgcat
      301 tttgcgcggc gaggaggaat aagctatgtc taagaccatt tctgcaggac gcaccccaag
      361 gatcgaaatt acagcagtca gtggtgatct gagcttggta gggtgggagg gagatgacat
      421 tctcctcaag gcagatgacg aagaaatgaa cgtcaagcaa gatggcgatc aggtcactat
      481 tgcttgtaaa gatgatcttt cgatgcgcgt ccccaaaggg tcgaccgtca ttattcaccg
      541 cattacgggt gatgcttctg tgcgtggggt gatgggagcg attgaattaa aggaaatctc
      601 tggtgacctt tccatgcgcg atgtagtgac cgtggcaatt gatacggttc attcggactt
      661 tagcctgcgt ggtgcaaaag gaagtttatc tatccgtaaa gcgggctcag acgtttcggt
      721 ccgcgatgtg gatggaaatg tagccattga atcggtttca gatgaccttg ccttgcgtga
      781 tgtgcgcggt agtgtcactg ccaatgtggg tgaagatgtg gtcatgtatt tgaatccgca
      841 ggcagggaat acctatgccg tgaatgcggg cgatgacatt ttattagtga tgccgcccaa
      901 agcaaatgcg accttaactc tcagtgctga cgaaatcgat gtagattggg atggcgtaga
      961 aaatgaagaa gatgccacca gccgcgtgat tatccttggc gatgggtctg ccaacttaac
     1021 tttgagtgca ggcggcgata tccgcgtttc gaatcggtct gatgctggtg aatcggctga
     1081 agattttggc aacttcgccg gcatgggcat ggattggtct ggttttggcg agcgtatctc
     1141 gcgccgtgta gagcaggcaa cgtctcgcgc ccaacgcaag gcagaagaag ccgcccgccg
     1201 tgcagaaaac aaaatgcgcg atgcagaacg acgtggacgc ggcaaagtgg cttttggcgt
     1261 aggacgttgg aattgggatt tctcacctaa aggggtgccg gttccaccga aacagcaagt
     1321 ttcagatgaa gaacgtttgg caattttgaa gatgcttcaa gaaaagaaga tcaccgcaga
     1381 agaagcagaa aaacttttag cagcacttga aggaggttcc taatgtcttc tgaagaacgc
     1441 agaaaaattc tgcaaatggt tgcagacgga aaaatcagtg cggaagaagc cgctaccttg
     1501 atgcgtgcat tggacgactc taacgctgaa gaggcttcag cggagatgga gattatcgaa
     1561 gtgcgccccg cttcgagcgc ggaaggagcc gggtcttcat atcaagaaaa gagtcatgcc
     1621 cctgaattag atgctgttcg tatgcgcgct catcggttct ctggcgcttt cctttggatc
     1681 ggaacgatcg taacggtttt gatcgcgtgg tggatgttca atgttcagca aaattctggg
     1741 ttgaattttt ggttcttctg tctaagcttg ccattgaccc ttggtatttt attgatcgcc
     1801 ttgggtgcgg gcagccgtac atcgcgttgg ttatatgtga atgtagatcg ttcgcagtca
     1861 aaggaccaag atggtccgcg caagatttca ttggcattcc ctttgccact tggttttgca
     1921 agttggtttt tgcgaacagt gggacgacat attgaagaac tacgcgataa gaatgtagat
     1981 gccattgccg acacgattgc tttggctaag aatatgacgg aaccgttgat cgtacatgtg
     2041 gacgatgaca gtgatggtac gcaggtgcaa gtttttatag gatagaaaaa atggaaacca
     2101 tcaagaaaca acctttatta agtggcatat tgatcttgtt tctaacggca atgatctttg
     2161 cgaacattgg cggaaatatg tatgaagggc tgatgccttt gtatttgaag gaactgaaag
     2221 cggatattac cgatatcggt ctgttcttta cattggctca gatcgtgcct ttgttccttc
     2281 agattttagg tggttgggtt tcagattctt taggacgttt acgtgccatt gcgattggta
     2341 gtgtgtttgg tcttttaggg tttattccgt tggtgttggc agatacctgg cactggttgt
     2401 tgattgctac tgctgtaggt gctattgctc gtgctttggt cggacccagc tttgacgctt
     2461 tcattgcgga acactccaca gaagaaaacc gtggcaaggt gtatggtatt tcgcaggcga
     2521 tctttatgat cgttaccgtg gtcggacctc ctttaggtgg ctggttgacc ggcgcctatg
     2581 gtttcaaatt gatgttattc attgcaggcg tgttttatgc cattgcggta gcaatgcgtt
     2641 tgagcatggc gcgcgaagcg acgaaaggcg aaaaagagaa tggtgaaaag aagagtcttt
     2701 cctttacgag cttgaaagaa aacttgggct ctatgattgc gttgattttc tccggcggct
     2761 tgatcacttg gatgttgatt accgatggtt tacgcgatac gtcgtttcaa ttctctggaa
     2821 atttgttccc ggtttatatg caagagatcg gtggcttgtc ttatcaacag atcggctggg
     2881 taacttcgat gttcggtatt tttatgatgc tgagcaccat ccctggcggt tggctttccg
     2941 ataaggcagg ggagcgggtg gggattgcgg ctggcatgtt cttgatgtct gtagctttgt
     3001 tcatcttgat tgtcatccct caagggacgc agtggcagtg gttattcttt gtggggtggg
     3061 ctgtggctgg cttgggggca gggatttccg gtccggcgta tcaatctttg atcagtaaag
     3121 cggttccaca aaaattgcgc ggacttgctt tcgggctgtt tagcaccagt ttgggcatcg
     3181 tatcgcttcc ggctccgtgg attggggctc agttgtgggc tcgttttggt ccgacggctc
     3241 ccttcaagat tactgctact gttattttgc tttcgatcat cccaatctgg ttaaaattca
     3301 aacttccaaa aacaactgaa gaaacccagc ccgaagcaga gcagccagta gaagaaaata
     3361 aagtcgctat cgcggcagga taaaataagc gttcgtcctg aacggcgaaa ggagaaatgg
     3421 aacatggcaa acgtagaaga aagaatgaaa attctgaaat taattgagga aggcaaaatc
     3481 agtgcggagg agggcgctaa gttgctctca gctctgaacg actcgcggaa gaatattccc
     3541 acgcctccgc gcccgccttc tgctcccggt ggtgcacgca tgttgagagt ccgcgtgaca
     3601 gatacccgca ctgggcgttc caaagcctct gttcaaattc cactggcgtt ggtggatgcc
     3661 gggttgaaga ttggtgctca ttttgctcct gaagttcagg gtgtagacat gagtaatgtt
     3721 atggaagcac tccgcatggg tgttaccggc aagatcattg acgtgaccga tgaagaagat
     3781 ggtgaacacg tcgaaatctt tgtcgaataa agtctgtcgg ttcgtgttgg ttatcagaag
     3841 gagaaaatta tgttcccatc actatacctt gtccatcgct tgatgttata gccatcaacg
     3901 cgcaagagat ttctcttgcg cgttgtatca tttttatcac agatcatcaa aggatacttt
     3961 tatgcaagag acaaagacaa atcgccccag tggtatgttc gcatttacga ttgtgtggct
     4021 ggggcagatc atttccgtgc ttgccagtgc gatgagtcaa tttgggctta cgatctggat
     4081 gtacgaaaag acccaaagcg ctactgcgct gggtttgatg caagtcttct tcattacccc
     4141 attcttgatt atttctccca tagctggtgt gatggtagac cggcacaacc gtaagttgat
     4201 gatgatggtt agcgacttaa ccgctggact tgctacgatt ttgattttaa tctttcagtc
     4261 gttgggtgtt ttgcaattct ggcatttgta tgtagcttcg atcatttacg ggttgggaaa
     4321 tgctttccaa tggcccgcat attcggctgc aatttccacg atggtgccga aggatcagta
     4381 cggtcgcgca aacggcatga tgtccctaat cgaggctgga cccggcgttt ttgccccgct
     4441 attagccggg gcgttgctgc cattgatcgg tttgactggc atccttgtta ttgatgttgt
     4501 gacattttca tttgcagttg gtgccttgtt gatcgtccac atccctcaac ctgaacgaac
     4561 ggtggaaggt gttcagggaa aaggcagtgt ttggcaggag gcggtttttg gtttcaaata
     4621 tatttttgcc cgccccagcc tgcttggttt gcagatggtg tttttctttg gaaatttatt
     4681 ttcgggtatt ctgttcactt tattagcgcc catgatcctt gcaagaaccg ataacaacag
     4741 cttaatgttt ggtacggttc aaacggcagg agcgattgct ggtgtagtgg gtggcttgat
     4801 tatgagtgcc tggggcgggt ttaaacgcaa ggtacatggc gtgttaggtg gatgggcaat
     4861 tacagggctt gggtctgctg tgattggctt gagttttggt ctgcctgcat gggtgattgg
     4921 cgttgctatt gcttcgttga ttagtccgct tgtgaatgca tctaatcagg ctatttggca
     4981 atcaaaggtg gcaccagatc ttcaaggacg tgttttttcc gcacggcgtt tgattgcttg
     5041 gttcacaaat cccatttctc ctattgttgc tggaacactg gcggattatg tccttgagcc
     5101 gcaaatgcga gccggtggga cgctttcgca gacctttagc tggttggtgg gcaccggtcc
     5161 cggttcaggg atggggctga tcattttggc atgcggtgtg ttttgcgtct tgatcggcgt
     5221 agcggggtat ttcatccctg caatttacaa tgcagagacg atcttgcctg atcatgatca
     5281 actggcaaaa gtagaacctg cataacagaa aataaaaaag cggagttgag atatctcaac
     5341 tccgcttttt gttctatcta atcttctgca gcgcgggcta cttcttcggg ttcgaagacg
     5401 acttcttctt tgccggtgag tttgg
//
LOCUS       DAOQNJ010000113         5420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_8924_length_5420_cov_9.058155, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000113 DAOQNJ010000000
VERSION     DAOQNJ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8924_length_5420_cov_9.058155"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLG52_04700"
     CDS             complement(<1..268)
                     /locus_tag="PLG52_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40775.1"
                     /translation="MLGSVHLERGKETLEFARQKTLLLLAYLALHPQEHPRERIASIF
                     WKDATTDEAARLSLRVTINDLRKTLGEDSLIGGRDSLQLNPDLNL"
     gene            564..3845
                     /locus_tag="PLG52_04705"
     CDS             564..3845
                     /locus_tag="PLG52_04705"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB40776.1"
                     /translation="MAFSLWADEDEASSLANLRRHLYLLKKSLPKALQPSLIISTQTV
                     LFQFPANTWVDVLAFEQEPTSLQEIEEHALFYTTDLAQGAETDEFIFSRREELRNRYA
                     QLLRMLAQTYFNYAEYETSMYWVRKLIAQDPWDEEALRLYMTSDFILGNRTTALSTYK
                     SFEKKLRLEMDIQPMPETMALYRDILHNHLLRPVSSTKTEGETFISRLSEIQTLGNAI
                     QAHGQYGNFVIINGTAGVGKTTLVREALRRFLNSSKAKLLWGDCQPAGEEQPYSIWQQ
                     IFNAAVPLLARQQDVAQEWLNHLLPLIPDLGLLRADLFSPTRPDATELRTAMRQTLFA
                     LSESRPLIIVIEDAHWMDVPSLEFLRELAESSLNIPVLFILTHRTDDAALPLFEIKRI
                     LRRKRNVVDISLQPFTTTETNQYLEKFLGTKTLPKELIDDLMQYSGGLPLLLHEVTQT
                     LNSSKRILSLREAFLFRLDRLSPAAREMVETAAVIGFSLTDIELRTALGWAAELYAST
                     MDALQAERFLLENPSNSADEYAFSHRLIHEIIVEQISVERISNLHIRVARALEQIHSE
                     DIGFAAEIALHYQQAGQLILAAGFWVRHASESTDLAAFDSGLAAINQAEAILTGASLD
                     AQEVHAKLALQRGVIVFYQGKSEEAFSLLEKAVEHSKLFPPLYAHALSMQAYAFHTHD
                     RNELAFQAASQAFDLSMATKDVSNAVRALNIRAVSALMLGKTRGALDDLQQALALLEQ
                     HNLSASTQTVQSLYHLGTALVFSQDYQQARAILSRAVELSKKGGLRRLEAAALTMLGQ
                     IALNCGQYKLAVEIYDRSIEVAGESYLPGMWGKFAGRGWANLRMGNLLAARTDFERGL
                     DVSIQVESQYGTILMRSYLAFTALAAGESPSASFADLEADALTQALHPVVLFNANIQS
                     QGWRLFGDSEKANSAHHRAIEAAKNSDVPSFVQSARLQKLYTQVILEENIDPTYMEEE
                     LAELAKLTLDSGEVPLQVNTFLVRAWLHSRMGNLQEALSTATQALTLARACPDLPWIG
                     ECQVLLAQLFTKLGDQHQAKICTEEVHTLAKTSFAPLTVKFGGEPLALVQEHLLKSLT
                     PTHLN"
     gene            3948..4565
                     /locus_tag="PLG52_04710"
     CDS             3948..4565
                     /locus_tag="PLG52_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40777.1"
                     /translation="MRNLLLAIFILALASMACNMPGSAGSPVNPPIEIPTFTATVPSV
                     VSDKPTDLPAPATSTPEQAVLQPQVNTFDPRSIADCDIFVDSDFPNTIGALPDTKKAL
                     PETDKKACQYNFANGTLFVSIFTGMPGMEAFENVRQFDALTGATISPYPIGEAAVFKD
                     FGDGHITLEAVINGWYVVLDARGFDEKNLALLAELLLANLAPYSP"
     gene            4578..4811
                     /locus_tag="PLG52_04715"
     CDS             4578..4811
                     /locus_tag="PLG52_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40778.1"
                     /translation="MNERRKAASFLIRVWQEPSQWQPPGEWRVSAHPLEGGGEVLFNS
                     ATELWRYLTHADSQIQSTPQTKKIISEGEENEQ"
     gene            4801..>5420
                     /locus_tag="PLG52_04720"
     CDS             4801..>5420
                     /locus_tag="PLG52_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40779.1"
                     /translation="MNNKRSLGCVLTGALIILVICGAIAGFATWRLNTRGPEILVEIK
                     RPQTTMLANVNEALPLIVSAEASDIITRLEVYADGALIAATNGEGQNAAMLAQTWTPS
                     TVGRHVLVARAFLDAEHFSDSEVVFVDALDLSALPLQVNVDDLPRGEGVTEISVGDLA
                     TASGTTPEEIVRLNPSLSSTGSIPSGTLVSIPRLAPEPGIGSPVPPP"
BASE COUNT         1422 a         1270 c         1264 g         1464 t
ORIGIN      
        1 ataggtttaa gtcagggtta agttgcaacg agtcgcgacc gccaattagg ctatcttcgc
       61 cgagagtctt acgcaagtcg ttgatcgtca cacgaagcga aagcctggca gcttcgtcgg
      121 tagtagcatc tttccaaaat atggaggcaa tccgttcgcg cggatgctct tgcgggtgga
      181 gggcaagata tgccaataag agtaaagttt tttggcgcgc gaactcaaga gtttccttcc
      241 cgcgttcaag gtggactgac ccaagcaagt aaattttgat tgtaggtgac tgtgacatat
      301 taaaagagga aatcaaagat tattaagatg tgttttttac agagtgtacc atgccccctt
      361 aatagggtcg ccattccaaa tcttttaatg tgaatcgtta taatttcttc atgtcgcagg
      421 catacaggat tcgcattttt ttatttggat cgcctaggat tgaagttaat ggcgttatat
      481 ctgccctgcc tgcacgcgat aaattactat gtctattcac ccgacttgtc cttcaggcga
      541 attccccaca ggggcggaaa catttggcat tctcactctg ggcagatgag gatgaagcca
      601 gttctcttgc gaatttaagg cggcatcttt acctcctgaa aaaatctctt ccaaaagccc
      661 tacaaccatc attgatcatc tctacacaaa cggttttatt tcaattccct gctaacacct
      721 gggtggatgt gcttgcgttt gaacaggaac cgacttcttt gcaagaaata gaagaacacg
      781 ccctgtttta cacaacagac cttgcacaag gggcagaaac agatgagttc attttttctc
      841 gtcgcgaaga attgcgtaat cgatatgcac agcttttgag aatgctggca cagacatact
      901 tcaactacgc cgaatacgag acatctatgt attgggtgcg aaaactgatt gctcaagacc
      961 catgggatga agaagccctg cggttgtata tgacttcaga cttcattctt ggtaatcgca
     1021 caactgctct ttcaacttat aaatcatttg agaaaaagtt gaggctggaa atggacatcc
     1081 agcccatgcc agaaaccatg gcactatacc gagatatttt gcataaccac cttttacgtc
     1141 ctgtttcttc cacaaaaaca gaaggtgaaa cattcattag ccggttaagt gaaatacaaa
     1201 cattgggaaa tgcgatccag gcacatgggc aatacggcaa ttttgtgatc atcaatggaa
     1261 cagccggtgt gggaaagacc actttggtcc gtgaagcgct tcggcgattt ttaaattcat
     1321 caaaagccaa actactttgg ggagattgcc agcctgcggg ggaagaacaa ccttactcga
     1381 tttggcagca aatttttaat gccgcagtcc ctttgcttgc gcgtcaacag gatgtggctc
     1441 aggagtggct aaatcattta ctgccgctca tcccagacct gggcttgtta agagccgatc
     1501 tgttttcccc tacccgtcca gatgctaccg aattgcgcac cgctatgagg caaactttat
     1561 ttgccttgtc agagagccgt ccgcttatca tagtgattga agatgcacat tggatggatg
     1621 tcccatcctt agagttctta agagagcttg ctgaaagctc tctaaacatt cctgttcttt
     1681 ttattcttac ccatcgcaca gatgatgctg cattgccttt gtttgaaatc aaacggatat
     1741 tacgccgtaa gcgaaatgta gtagatatct ctctgcaacc atttacaaca acggagacaa
     1801 atcaatatct tgaaaaattt ttgggaacaa agactcttcc aaaagaactt atagatgacc
     1861 tcatgcaata ctcaggtgga ctgcctttat tattgcatga ggtgacccaa accctcaact
     1921 caagcaagag aatcctttct ttgcgcgagg cgtttctctt tcggttagat cgtctctcac
     1981 cggctgcacg tgaaatggta gagacggctg ctgtgatcgg attttccctg accgacattg
     2041 aattgcgtac ggctctgggt tgggcagcgg aactttacgc ctccacaatg gatgctttgc
     2101 aagcagaacg gtttcttttg gaaaatcctt caaatagtgc agacgaatat gctttttccc
     2161 accgcctgat tcatgaaatt attgtagagc agatctctgt agagcgaatt tcaaatttac
     2221 atattcgtgt ggcacgcgca ttggaacaaa tccattcaga agatattggg tttgcggcag
     2281 agatcgccct tcattatcag caagccggtc agcttatctt ggctgccggc ttctgggtgc
     2341 gccatgctag cgaaagtaca gacctggctg catttgattc tggtctagct gccatcaacc
     2401 aagccgaagc cattcttact ggcgcaagtt tagatgctca agaggtgcac gctaaacttg
     2461 ctcttcaacg cggggtgatc gtgttttacc aaggaaaaag cgaagaggct ttttctttac
     2521 ttgaaaaagc agtggaacat tcaaaactat tcccgccact atatgcacat gctctttcga
     2581 tgcaagccta tgcttttcac acccacgacc gcaatgaact tgcatttcaa gctgcttcgc
     2641 aagcatttga cctttccatg gcaacaaaag atgtttcgaa tgcggtgcgt gctttaaaca
     2701 ttcgcgcagt gagtgccttg atgctgggaa agacacgtgg cgcgttggat gatcttcagc
     2761 aagcacttgc cttactggaa caacacaacc tttctgcatc aacgcaaact gtacaaagtt
     2821 tgtatcatct cggcactgct cttgtctttt cacaggatta tcaacaagca cgagccatac
     2881 tttcccgtgc agtggaactt tcaaaaaagg gcggactgcg tcgtctagaa gcggctgcgt
     2941 tgaccatgtt gggtcagatt gctttgaact gtggccaata caaacttgct gttgaaattt
     3001 acgatcgatc cattgaagtt gctggagagt cgtacctgcc cggtatgtgg ggtaaatttg
     3061 ccgggcgggg ttgggcaaat ttacggatgg gaaatctact ggcagctcgc acagactttg
     3121 aacgcgggtt agatgtttct attcaagtag aaagccaata tggaacaata ttgatgcggt
     3181 cttacttggc gttcactgca cttgcagctg gagaatctcc ttccgcttca tttgcggacc
     3241 tcgaagcgga tgcgctgacc caagccttac atcccgttgt gctttttaat gccaatatcc
     3301 aatctcaggg gtggcgctta tttggggata gcgaaaaagc aaattcggca caccaccgcg
     3361 cgatcgaggc tgccaaaaat agtgacgttc cttcttttgt tcaatcagca agattgcaaa
     3421 aactatatac gcaagtcatc cttgaggaga acattgaccc aacttacatg gaagaggaac
     3481 tcgctgaatt agccaagctg actcttgatt ctggcgaagt gccattacaa gtcaacacat
     3541 ttttggtgcg ggcgtggttg cattctcgta tgggcaattt acaagaagcg ctttccactg
     3601 cgacccaagc actaaccttg gcgcgcgcct gcccagacct gccatggata ggagagtgcc
     3661 aagtcttact tgcccagctt ttcacaaaac ttggggatca acatcaggca aagatatgta
     3721 cagaagaagt gcataccttg gcgaaaactt cctttgcacc tttaaccgtt aaatttggag
     3781 gagagccttt agcgcttgtg caagagcatt tattgaaaag tcttacaccc acacatttga
     3841 actagtcctt ccgtactaaa ctcttgaagg taaggggaaa cacttgttta gaggacgttt
     3901 ttaggacgct ctctctgtaa gatatggggc aaaaaggaga gaaaagcatg cgaaacctgc
     3961 tattggctat ttttattttg gctctggctt cgatggcttg taatatgcct ggctcagccg
     4021 gctcgcctgt caacccgccc attgagattc ctacttttac agctacggtt cccagtgtag
     4081 tgtcagataa accaactgac ttgcccgccc ctgcaacatc cactccagag caagctgtat
     4141 tacaaccgca agtcaacaca tttgacccgc gcagtatagc tgactgcgat atttttgttg
     4201 actccgattt tccaaacacc attggcgctc ttccagatac gaagaaagct cttcccgaaa
     4261 ctgataaaaa agcctgccaa tacaattttg caaatggcac actctttgtt tccatattca
     4321 ccggcatgcc ggggatggaa gcctttgaga atgtccgtca gtttgacgct ctgacaggtg
     4381 caacaataag cccataccca atcggtgaag ctgccgtatt taaagatttt ggtgatggac
     4441 atatcacact cgaagcagtc atcaacggtt ggtatgtggt attggatgca cgcggcttcg
     4501 acgaaaaaaa tctggcactg ctggcggaat tattgcttgc caatctggct ccctattcgc
     4561 cttaacggag cttatccatg aatgaaagac gaaaagcagc atcttttctg attcgggttt
     4621 ggcaggaacc gagtcagtgg cagccccccg gcgagtggcg agtaagcgct cacccgttgg
     4681 aaggaggcgg tgaggtcttg ttcaactcgg caactgagct atggcgttac ctcacccatg
     4741 cagactcgca aatccagagc acccctcaaa caaagaagat tatttcagaa ggagaagaga
     4801 atgaacaata aacgcagtct tggttgtgtt ctaacgggag cgttgataat tcttgtaatt
     4861 tgcggcgcca tagcaggctt tgctacgtgg cgattgaata cacgcggacc tgaaatcttg
     4921 gttgagatca aaagaccaca aacgaccatg cttgctaatg tcaatgaagc gttgccgttg
     4981 atcgtctctg ctgaagcaag cgatattatc acccgtcttg aagtgtatgc agatggtgct
     5041 ttgattgctg ccaccaatgg tgaggggcaa aatgctgcca tgttggcaca gacctggacg
     5101 ccctctacag tcgggagaca tgtgcttgta gcgcgagcat ttttagatgc cgaacatttt
     5161 tcggattcag aagttgtttt tgtggatgcg cttgacttga gtgcattgcc acttcaagtg
     5221 aatgtagatg acctgccgcg cggtgaaggt gtgactgaaa tttcagtggg tgacttagcc
     5281 accgcctcgg gaaccacgcc ggaagaaatt gttcggttga acccaagtct ttcctctact
     5341 ggaagtattc cttcaggcac gcttgtttct attccgcgcc ttgctcccga acctggtatt
     5401 ggtagccctg tgccaccgcc
//
LOCUS       DAOQNJ010000114         5383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9042_length_5383_cov_6.636261, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000114 DAOQNJ010000000
VERSION     DAOQNJ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9042_length_5383_cov_6.636261"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1196
                     /locus_tag="PLG52_04725"
     CDS             228..1196
                     /locus_tag="PLG52_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40780.1"
                     /translation="MDIATLEKYPVNWLETSLKKRNIPWILFILLICAFLFAIEASYS
                     LRPDVDNFVGIAVYDVVIEAYALGAMGVVRSFLTQALVAIYPLIDNYQELTPTPHREW
                     QYILGVGGVLTVIAVTTNPLVSNTLNEPTTWLYILSQLIENSLLGWIVFSLLASVGVI
                     THFISQVKTANIFDSAPYRPIAHWCLVVEISIMGAVTIAMLFLQQDMMSSINLVTYIV
                     IGILGVLIFFGGMWSTHQHMLNNKEKEISIINQELHKLHQEILTAIRDKDFEKSHAYS
                     ETSADLVTHRERIEKVPDWPYTIGSLGGLASSVVIPVAINFLSKII"
     gene            1220..1492
                     /locus_tag="PLG52_04730"
     CDS             1220..1492
                     /locus_tag="PLG52_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40781.1"
                     /translation="MAGKYAPLEAYFRALPKDKREVKLSFEKIEEILTFKLPASAYED
                     ERWWLHEKEGNHVNLRAWANAGWKMAKVDVLKRQVLFGRTKVVKGS"
     gene            1533..1892
                     /locus_tag="PLG52_04735"
     CDS             1533..1892
                     /locus_tag="PLG52_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40782.1"
                     /translation="MAFTVRIIGVPVQKDLTNWVVDHVLWWPVDEWIGKASGERNAPV
                     FRESVDSTLYLDQIGIFNKEEFIRLNEEYKAAFLADARRQPIHAEQQVRMKEVEAYLK
                     QRTDTKWILVEKYEWES"
     gene            1959..2507
                     /locus_tag="PLG52_04740"
     CDS             1959..2507
                     /locus_tag="PLG52_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40783.1"
                     /translation="MPYDPSDPTARDSALQPSEQRRLEEANFYFENNKPAQAAPIFAK
                     LAEVLRSAGQPKRAASLHSQAALAFARSRNESALVQARAAMSLLLQYKLEAQASAFYK
                     ELMREFTKRGMQPTAEAFAKEFGGQIQQPAEQDAHAAHQLDRLPSHCPSCGASLRERD
                     IRWTDADTIECGFCGTPIRPSV"
     gene            2550..2969
                     /locus_tag="PLG52_04745"
     CDS             2550..2969
                     /locus_tag="PLG52_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40784.1"
                     /translation="MKPKMMLGCLLVGLTLLVGCGPKETPLSGPERDAVLAFSEAKTD
                     NLLAGMNASDYAAFSQDFDQEMLAAIPQSAFETLKADRDATLGLYVSRTVNTVVQSGE
                     FYAVVYDAVFEKDEAVTVRVVFRVAEPHQVSGLWFNK"
     gene            2993..3220
                     /locus_tag="PLG52_04750"
     CDS             2993..3220
                     /locus_tag="PLG52_04750"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNB40785.1"
                     /translation="MTETSQPNNPLHGITLETIVTQLVQQYGWAELGKRIPIKCFTND
                     PSVKSSLKFLRQTPWARKKVEDLYLQSVGKG"
     gene            3229..3723
                     /locus_tag="PLG52_04755"
     CDS             3229..3723
                     /locus_tag="PLG52_04755"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNB40786.1"
                     /translation="MRIGRILFVGVLVLCLFEMARLWTISPLQMAAHFNALGEPDRFV
                     PKSEFFWGQVQTLFVVVLVSLPLQVLFLVIPPNLINMPNREYWLAAERKDETLGRLSD
                     FGSMLFAVILLALLAGFEIATYANLQTPIHFNAGLMGVVMFAAMGVIVLLLIQLVLSF
                     KLPK"
     gene            complement(3774..4688)
                     /locus_tag="PLG52_04760"
     CDS             complement(3774..4688)
                     /locus_tag="PLG52_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40787.1"
                     /translation="MKTYKLWIGSIILALTLQACNLKLVDAGSDPNAPGYELTVTANA
                     ALIETAMASGAQPPADAGQPAGVDQPPAAVDAPPPTEDPNNASLNTATPTVSSTNGTT
                     VTVSIATNCRTGPGQAYPSIYGLPVGVVGKVVGNNPSVSNYWVIEIPNSGGGTCWLWG
                     QYATVTGDTNTLKVVAVPATPTPTATLTPTATKVVAPAAPSNATSVLLTCVTDPNSFI
                     QTFTATFTWTDNSNNETGFNIYVNANFANGSSVDILSGSVGPNTTSYTVSTTALSKQP
                     PIIKVEAFNNAGSSTRVSAQPLSLATCP"
     gene            complement(4779..>5383)
                     /locus_tag="PLG52_04765"
     CDS             complement(4779..>5383)
                     /locus_tag="PLG52_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40788.1"
                     /translation="YATINGDTAGLKDVVTPTSVATLKPTATATATATSTTSAGVPAV
                     AGCTNSAASNYNSAATVDDGSCVFTAQATILGCTDQGAANYNRNATADDGSCIYDVIV
                     TNTSVSCEDQLDGNYKFTYTINWIKNNTVSGLEYYIKYRWEEGDETPRSENETVPVST
                     SPTIVTAIKPRNLRLSFWFIVVNPLTTVRTTTNPIRIKCP"
BASE COUNT         1302 a         1242 c         1433 g         1406 t
ORIGIN      
        1 cttcctcttt cttattgcct ctggtttttg atttgatcaa caagctattc taggtttaca
       61 taatcgctaa aataaataaa accctatcta gtagcgccca aaattgactt ccgtcgcact
      121 tgcttctaca atgggcttgg gagaaggtgg gggagagtgt ttggcttggg ctacatccgc
      181 agcatttctg aataaagaaa aacaaacgcc tcttcttgga gaacatcatg gacattgcca
      241 ccctcgaaaa atatccggtc aactggctgg agaccagcct caaaaaacga aatattccct
      301 ggattctttt catccttttg atatgtgcct ttttgttcgc cattgaagcc tcttacagcc
      361 tccgcccgga tgtggataac ttcgttggca ttgccgtgta cgatgttgtg attgaagcct
      421 atgccttggg cgctatggga gtggtccgct ctttcttaac ccaagccttg gttgccatct
      481 accccttgat cgataactat caagagctca cacccactcc acaccgcgag tggcagtaca
      541 ttctgggtgt gggcggcgtg ctgactgtga ttgccgtcac gacaaatccg ctcgtgagta
      601 atacgctcaa cgaaccgaca acctggttgt acattctcag tcaactcatc gaaaacagcc
      661 tgctcggctg gattgtcttc tctttgcttg ccagtgtggg ggtaatcaca cacttcatct
      721 cacaggtgaa gaccgccaat attttcgatt ccgctccgta tcgtccgatt gcacattggt
      781 gtctggtagt ggagatctcc attatgggag ccgtgaccat tgccatgctc ttcttgcaac
      841 aagacatgat gagcagcatt aacctggtca cctatattgt gatcggtatt ctaggggtgc
      901 tcatcttttt tggaggcatg tggagcaccc atcaacatat gctgaataat aaagaaaaag
      961 agatcagcat cattaatcag gaattacaca aactgcacca agagatcctc acggccatca
     1021 gagataaaga ctttgagaaa tctcacgctt attccgaaac cagcgccgat ctggtgacac
     1081 atcgagaacg gatcgaaaag gtgcccgact ggccttatac aattggcagc ctgggcggct
     1141 tggcaagttc ggtggtcatt ccggtggcga tcaacttctt gtcgaagatt atttaaagta
     1201 gggagaacca tacagtccta tggctggtaa atacgctcca cttgaagcgt actttcgggc
     1261 gcttccaaaa gataaaagag aagtgaaact atctttcgaa aagatcgaag agatattgac
     1321 gttcaaactg cctgcgtctg cctatgaaga tgaacgttgg tggctgcatg agaaagaagg
     1381 caaccacgtc aatttgcggg cgtgggcaaa tgcgggttgg aagatggcaa aggtagatgt
     1441 tcttaagcgg caagtgctgt ttggtcgcac caaggtggtt aaaggatcat agagatataa
     1501 tttttgagcg cagcaaagac gaaggagtac acatggcatt tacagtcagg attatcggag
     1561 tgccagttca aaaggatctt accaactggg tcgttgacca tgtgctttgg tggcccgttg
     1621 acgagtggat cgggaaagca agtggggagc gcaatgcccc tgtatttcgc gaaagcgtag
     1681 atagtacact ctatctggat cagatcggta ttttcaacaa ggaagaattt atccgtttga
     1741 atgaggagta caaagcagcg tttctggcag atgccagacg gcagccgatt catgctgagc
     1801 agcaagtgcg catgaaagag gttgaggcgt atcttaaaca acgtaccgat actaagtgga
     1861 ttctggtgga aaaatacgag tgggaaagct aacaaaaagt gttgagatga gaaaaatgta
     1921 ggataacccg tgatgtgatt tcgtgagaga atgtctgcat gccatacgat ccctctgacc
     1981 ccactgcacg cgatagcgcc ctacaacctt ctgaacaacg ccggctcgaa gaagctaatt
     2041 tctattttga gaacaacaag ccagcccaag cggcaccgat ctttgccaag ctggcagagg
     2101 tgttgcgttc tgccgggcag cccaagcgtg cggcaagttt acattctcaa gccgcgctcg
     2161 cctttgcccg cagccgaaat gagtccgcgc tggtgcaggc tcgggcggca atgtctttgt
     2221 tgctgcaata taagctcgaa gcgcaagcgt ccgcgtttta taaggagttg atgcgtgaat
     2281 tcaccaagcg gggaatgcaa cctacggctg aggcgtttgc caaagagttc ggtgggcaga
     2341 ttcagcaacc ggcggagcaa gatgcgcatg cggctcatca gctagaccgc ctgcctagtc
     2401 attgtcccag ttgcggggcg tctctgcgtg agagggatat acgttggacg gatgcagaca
     2461 ccatcgaatg tggcttttgc ggcacgccca ttcgcccgtc tgtttaaaac gtaacatagc
     2521 ggtcttataa aaaaggatga tgaattgcca tgaaaccaaa aatgatgctt ggatgcctgt
     2581 tggtgggtct caccttgctg gttgggtgtg gaccgaaaga aacgcctctc tctggaccgg
     2641 aacgggatgc cgtcctcgcc tttagcgaag ccaagacgga caatctgctg gcagggatga
     2701 atgccagtga ttatgccgcc ttctcgcaag actttgacca agagatgttg gctgccattc
     2761 cgcaaagcgc tttcgagacc ttgaaggcag accgcgatgc tacattaggg ttatacgttt
     2821 cacgcacggt caacactgtg gtgcaaagtg gagagtttta tgcggtggtc tatgatgctg
     2881 tttttgaaaa ggatgaggct gttacggtgc gagtggtctt ccgtgtggca gaaccgcatc
     2941 aggtcagcgg gttatggttt aacaagtagc tctggcgtac actggaattg ttatgacaga
     3001 aacttcacaa cccaataatc ctttacatgg cattacgctt gaaacgattg ttactcaact
     3061 cgtacaacaa tacggttggg cagagttggg caagcgcatt ccgatcaaat gctttacgaa
     3121 cgaccccagc gtcaaatcga gcctcaagtt tttgaggcaa acgccgtggg cgagaaagaa
     3181 agtggaagac ttgtacctgc aatccgtcgg caaaggctaa ggtgccccat gcgtattggg
     3241 cgaattcttt ttgtaggtgt gttggtgctc tgtctcttcg agatggcgcg tttgtggacg
     3301 atcagtccgc tgcagatggc ggcgcatttc aatgccttgg gcgagccaga ccgcttcgtg
     3361 cccaagtccg aattcttttg ggggcaggta caaaccttgt ttgtcgtcgt gttggtcagc
     3421 ttgcccttgc aggtcttgtt tctggtcata ccacctaact tgatcaacat gcccaaccgc
     3481 gagtattggt tggctgccga acggaaagat gaaaccctcg ggcgtttgag cgatttcggc
     3541 tcgatgctgt tcgctgtgat tctgcttgcg cttctggctg gttttgagat cgccacatac
     3601 gccaatttgc aaacgccgat ccacttcaat gccgggttga tgggggtggt gatgttcgct
     3661 gccatggggg tgattgtttt gctgttgatt caactggtct tgtctttcaa attgccgaaa
     3721 taaaaaagag acggtcacaa aatgaccgtc tcttttttga attaacgatt cgcctaaggg
     3781 caggttgcaa gactcagggg ttgggcagat accctcgttg aactgcccgc attgttaaac
     3841 gcctctactt tgatgatagg cggctgctta ctaagagcag tagtgctgac cgtataactg
     3901 gttgtatttg gacccacaga acccgatagg atatcaacag aactgccgtt tgcgaagttc
     3961 gcattcacat agatattgaa cccggtctca ttatttgaat tatctgtcca tgtaaaggtg
     4021 gcagtaaagg tttgtataaa actattagga tcggtcacac aagtcagcaa aactgaagtg
     4081 gcattcgaag gcgcagcagg cgctactact tttgtcgctg ttggggtgag cgtggctgtt
     4141 ggcgtagggg tggcaggtac ggcaactacc ttcaatgtgt ttgtatcgcc ggtcacggtg
     4201 gcatattgcc cccacagcca gcaggttccg ccgccgctgt tgggaatctc gatcacccaa
     4261 taattggata cagacggatt gttaccgacc acctttccaa ccacacctac cggcagccca
     4321 taaatactgg ggtaggcttg accaggtccc gtgcggcaat tggtggcaat gcttacggtg
     4381 accgtagtgc cattcgtgga tgaaactgtc ggcgtggcgg tgttcaagga tgcattgttc
     4441 gggtcttcgg taggaggagg cgcatcgacc gccgccgggg gctggtccac accagcaggc
     4501 tgacccgcgt ccgcaggcgg ttgtgctccg cttgccatgg cggtttcgat caaggcggca
     4561 ttggcggtca cggtcagttc gtagccgggg gcgttagggt cactacctgc atccacaagt
     4621 ttcaggttgc aggcttgcag ggtgagtgca agaatgatgg acccgatcca aagtttgtat
     4681 gtcttcatgc tttttcctta tggactggtg aatgaaaccg aagttgaatt ttcaaacgta
     4741 acggtgacga ccgtctcttt tttgattaac tcatctgctt acggacattt aattctaatc
     4801 ggatttgttg ttgttcgtac tgtggttaat ggattaacaa caataaacca aaagctcaac
     4861 ctcaaattcc taggtttgat ggctgtcact atagtaggcg aggtagagac gggaactgtc
     4921 tcgttttccg acctgggggt ttcatctccc tcttcccacc ggtatttgat gtaatattca
     4981 agtccgctaa cggtattatt cttaatccag ttgatggtgt aggtgaattt ataattgcca
     5041 tccaactgat cttcacaaga tacagatgta tttgtaacga tcacatcgta tatacacgat
     5101 ccatcatcgg cagttgcgtt acggttgtag ttcgctgcgc cttggtcggt acagcccaaa
     5161 atggttgcct gtgcagtaaa gacacacgaa ccatcgtcca cggtggcagc agagttgtaa
     5221 ttgcttgcgg ctgagttggt acaacctgcc acggctggaa caccagcaga tgtggtgctg
     5281 gtggctgttg cggttgccgt tgccgttggt ttcagagttg ccactgaggt gggcgtgact
     5341 acatccttca agccagctgt atcgccattg atagttgcgt att
//
LOCUS       DAOQNJ010000115         5382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9046_length_5382_cov_10.359865, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000115 DAOQNJ010000000
VERSION     DAOQNJ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9046_length_5382_cov_10.359865"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLG52_04770"
     CDS             complement(<1..1028)
                     /locus_tag="PLG52_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40789.1"
                     /translation="MNEFTQNTPVVEKKHSNGALALFLIVLLPMPFCLFIYNFIVWST
                     EQSAIASSSMKQLAWAGFIGLGVQIFLMSLVFGLLWYLTSDDRFKGWYASLLAASLFG
                     IPALLLRALGPNNDQLGSILQFVLAVIVALAVIYIRKDKLVWDFSALPFGFLVAALGF
                     APLAVYGSFGSPADVVLSLFAGLAFGLLAAVLMSDAENIFLNGIGAGGLLALLASSFG
                     YDGSQLILVVLVPAFGCAIAALYPSRAAATVAAGLLAFAGLAFFDPTELTIVLGDIGG
                     LAVKAVGIALLIGWGVSVVVLILRQVMGSGSTSGVKRAAGWVGAVAGWVMVIAAFFIW
                     GNPGNYSDR"
     gene            complement(1054..1707)
                     /locus_tag="PLG52_04775"
     CDS             complement(1054..1707)
                     /locus_tag="PLG52_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40790.1"
                     /translation="MIKPVRWNTFIRDFIRIQIGFLLYGLAITLMIRGNLGTSAWAVL
                     EVALADFLHITVGTMTVIMGFIVLILSLLMREQIGWGTLGNILSIGPWVDLCMALVPT
                     VNNNLAIQILMLLSAIIIMGLASAIYIGVDAGAGPRDSLMLAIRRKTGVSIRVSRAII
                     EVTVVVLGWLLGGPAGIGTVVHAVLIGPSVQWGFKVFKVQAHTEREEVQAGIEGGAE"
     gene            complement(1770..5369)
                     /locus_tag="PLG52_04780"
     CDS             complement(1770..5369)
                     /locus_tag="PLG52_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB40791.1"
                     /translation="MDSIHPDDIGAVLATMNNLVGDSSSPRTVQYRYRHQDGSYHWVE
                     SIFSNQFAEPAVEAIVVNFRDITERKEMERALYESERYYRALIENTADGILVIDGSGV
                     VTYESPSVARLLGYGQDKLIGTSSFNLIHPDDLSEILKTFVDGLNSPGFVHRGEYRLL
                     NAENNWRYFEIVTHYLMDDPAIMGVIINGRDITERKQSESALKESERKFHGVIDKSAD
                     GIALSDEEGRILEFNEAFEQLTGKKRENVLGKFLWELAYELMPDSTKADDENFTLRNR
                     IQQALKIGATSYFQFTQEVPFQRADGSVLIIQQRIFTIRTDKGCRLGSISRDITEIKK
                     VEEYLTQQNKQLNSLYQITSALSQINAVEDIYAIALDSLCSNLDIKRVSILLFDRDGV
                     MRFKAWRGLSDRYRQAAEGHSPWKPDAVDPQPVLVEDAFTDAELASLYELFKSEGIGA
                     LGFIPLVHQNKLLGKFMIYFDAPHHFLETEVRLAQAIASHVAFAIHKSVLNEALRTSE
                     EQYRILYEDNPSIYFTTDDKGTILSVNKTGVEQLGYAIDELIGSPLLDIFYPDDREHV
                     LQKMLACINDPEKTVQVEARKQRKDKSILWVREFARAIRNTDGQLVILIVCNNVTEEV
                     KAEEALVQSEAELRALFSSMKDSVIVIDRNGIYQSVAPTNPETLYIPPAEVIGKGLSA
                     FFPEEQVQEFLKAIEYVLATGKTVIIEYQIILAEVSPWFESRVSPLGPDTTIWVARDV
                     SERKKIQSALIQSEQAYRSLFENMPIGLYRSSIHGELLDANPSLLRMFGYEDLETMKG
                     KKAWEFYNDPALDDEFRQQISENGFLNRYESEFIRQDGTRFWAEDYVRAVYDGKGIPL
                     YYEGSLIDVTDRKAAEKRLKESEKQYRLLAERIADVVWVLDLATLEFKYISSSVQNLL
                     GHSAEELLNKHVSMVLTPSAMMAIESITPERIKRFLAGDKTSVTELYQLDHVHKDGSI
                     IVVEVSSTFVMNENATIEAIGVTRNITERKKAEDDLRLANSSLQIAHKELQQLFEYEQ
                     ILARTDGLTRLYNRRYFFELAAREFNSAIRYQRPLTLIIFDVDGFKQANDTFGHDFGD
                     RTLVEVSKTANLQVREVDILARYGGDEFTILLPETNVEQAFLIAERIRKAVADVYIDM
                     GGFSTFVTISMGIAELSFEPQDQSIEDMIRRADQALYQAKQQGRNKVVIYSDK"
BASE COUNT         1568 a         1356 c         1045 g         1413 t
ORIGIN      
        1 cggtcactat aattgccggg gtttccccag atgaaaaaag cggcgatgac catgacccac
       61 cctgccacag cacctaccca tcccgccgcc cgcttcacac ctgacgttga gcctgatccc
      121 attacctgac gcagaatcaa caccacaaca ctgactcccc aaccgatcag caatgcaatc
      181 cccacagctt tcacggcaag cccgccaata tcaccgagca caatggtgag ttcagttggg
      241 tcgaagaatg ccaagccagc aaatgcaagc aacccagccg caactgtagc tgccgcccgc
      301 gatggatata aggctgcgat agcgcaacca aaagcaggaa ctaaaacaac caatatcaat
      361 tggcttccgt catacccaaa cgaagaagcc agtaatgcca acaaaccgcc tgcgccaatg
      421 ccattaagaa agatattttc agcatctgac attaacacgg cggcaagcaa gccaaatgcc
      481 aaacccgcaa acagactaag caccacatcg gcgggcgacc cgaacgagcc atacactgcc
      541 agcggagcaa accccaatgc cgcaaccaag aacccgaatg gcaacgcact gaaatcccaa
      601 actagtttgt ccttacgaat atagatcacc gccaaggcaa cgataacggc aaggacaaac
      661 tgcaaaatag aaccaagctg gtcgttgttt ggtccgagtg cacgcaaaag caaggctgga
      721 attccaaaca aggaagcggc taacaagctt gcataccatc ctttgaaacg gtcatcactg
      781 gtaaggtacc aaagcaagcc aaacaccagg ctcatcagga agatttgcac acccaaacca
      841 ataaaccctg cccatgccaa ttgcttcata ctcgaggagg caattgcgct ctgttcggta
      901 gaccaaacaa tgaagttgta gataaacaaa caaaatggca ttggcaaaag cacaatgagg
      961 aataatgcca aggcgccatt gctgtgcttc ttttctacaa ctggcgtatt ttgagtaaat
     1021 tcattcatgg ttcactcctt gatcgtttca catttattct gcgccgcctt caataccagc
     1081 ttgcacttct tctcgctcgg tatgagcctg caccttgaac actttgaagc cccattgaac
     1141 ggagggtccg atcaaaacag catggacaac cgtgccaatt ccggcgggtc cgcctaacag
     1201 ccagccaagt accaccaccg tcacttcaat gatcgctcgc gatacgcgga tgctcacgcc
     1261 tgtcttgcgc cgaatcgcaa gcatcaagct gtctcgtgga ccagcgcctg cgtcaactcc
     1321 aatatagatg gcgctggcaa gtcccataat gatgatggcg cttaacaaca tgagtatctg
     1381 tatagcaaga ttgttattca cagtaggaac caacgccatg cataagtcca cccagggacc
     1441 aatggaaaga atatttccta acgttcccca ccctatctgt tcccgcatta acaaggagag
     1501 tatcaaaacg atgaagccca taataacagt catggtaccc accgtaatgt gtaaaaagtc
     1561 tgccaatgcc acttccaata ctgcccaagc acttgtcccg aggtttccgc gaatcattaa
     1621 ggtgatcgcc aagccataca aaagaaagcc gatctgaatg cgaataaaat cgcggataaa
     1681 tgtattccaa cgaactggtt taatcatgat gctcctttga aagcatcatg ataccatgcc
     1741 gctataaacc ttgatttttt gtgatgcgct tatttatctg agtaaattac gactttattc
     1801 ctaccctgtt gttttgcctg atacaaagcc tggtctgccc ggcggatcat atcttcgatc
     1861 gactgatctt gaggttcaaa agataattct gcgattccca tgctgatggt aacaaacgtg
     1921 gagaaaccac ccatgtcaat atagacatct gcaactgctt tacgaatacg ttctgcaatg
     1981 agaaaagcct gctctacatt tgtctctggc aaaagaatcg taaattcatc accaccgtag
     2041 cgtgccaaaa tatccacttc acgtacttgt aaatttgctg ttttggaaac ctcaaccaat
     2101 gtgcgatccc caaaatcatg tccgaatgta tcgtttgcct gtttgaaacc gtctacgtca
     2161 aaaataatca atgtcaatgg tctttgatag cgaattgcgg agttaaactc gcgtgcggca
     2221 agctcaaaga aatagcggcg gttgtataaa cgcgtcaaac cgtcggttcg agcaagaatc
     2281 tgttcatact cgaacaattg ctgaagttcc ttatgggcaa tttgaagaga actattggca
     2341 aggcgcaggt catcttctgc tttctttcgc tcggtaatat tacgagtcac accaatcgct
     2401 tctattgtag cgttttcatt cataacaaat gtgcttgaaa cttcaaccac aataattgaa
     2461 ccatctttat gaacatggtc caactgataa agttctgtta cagatgtttt atctccagcg
     2521 aggaagcgct ttatgcgttc aggggtgatg ctctcaatag ccatcatagc agaaggagtt
     2581 aacaccatgc tgacatgttt attcagcaat tcctcagcag aatgccctaa aaggttttgg
     2641 acagacgagc taatatattt gaattcaaga gttgccagat ccaaaaccca aaccacatcg
     2701 gcaattcgtt cagccaatag gcggtattgt ttctcgcttt ccttaagtcg tttttctgct
     2761 gcttttcgat ccgttacatc gatcagactg ccttcgtaat aaagtggtat acctttccca
     2821 tcatacacag cacggacata atcttctgcc cagaatctgg tcccatcttg acggataaat
     2881 tcagactcat atcgattcaa aaaaccattt tcagaaatct gctgtcggaa ctcgtcatct
     2941 aaagcagggt cattataaaa ctcccatgcc ttcttcccct tcattgtttc taaatcttca
     3001 tatccaaaca ttcgcaacaa agatgggttg gcatccagca actcgccatg gatagaagaa
     3061 cgatatagtc cgattggcat gttctcaaac aaagaacgat aggcttgttc agattggatc
     3121 aatgctgatt ggatcttttt acgttcagaa acatctcttg ccacccagat ggttgtatcg
     3181 ggtcccaacg gcgagactct cgactcgaac catggcgaaa cttctgccaa aatgatctga
     3241 tattcgataa tgaccgtctt accagttgct agcacatatt caattgcctt gagaaattcc
     3301 tgaacttgct cttcagggaa gaacgcggat agtcccttcc cgatcacctc ggcaggaggg
     3361 atataaagtg tttccgggtt tgtaggagct acactttgat agatcccatt tcggtcaatc
     3421 acgataacag aatctttcat tgaagagaat aatgcccgta gctcagcttc agactgaaca
     3481 agggcttctt cagctttgac ttcttctgta acattgttac aaacgatcag aatcaccaat
     3541 tgtccatctg tattcctgat agcacgcgca aactctctca cccatagaat gcttttatct
     3601 ttcctttgtt ttcgcgcctc aacctgaacg gtcttttcag gatcgttgat acacgccaac
     3661 atcttttgca gaacatgttc gcggtcatct ggataaaaga tgtccaacaa tgggctgccg
     3721 atcagttcat caatggcata ccctaattgt tccacaccag ttttgttgac agaaaggata
     3781 gtgcccttat catctgtagt gaaatatatg gatgggttat cttcatacag aatacgatat
     3841 tgctcttcgc tcgtacgtaa agcctcattt aacacgctct tatgaatagc aaacgctaca
     3901 tgcgaagcga ttgcctgagc taagcgaacc tctgtttcaa gaaaatgatg tggggcatca
     3961 aaatagatca taaacttgcc aagcagtttg ttctgatgaa caagcggaat aaaaccgagg
     4021 gcaccaatcc cttcgctctt aaagagctca tacaaagaag cgagttcagc atcagtgaat
     4081 gcatcttcta ccaaaaccgg ttgcggatcc actgcatctg gtttccaagg agagtgtcct
     4141 tctgctgctt ggcgatatct atctgacaat ccgcgccaag ctttaaaacg catcacccca
     4201 tctctatcaa ataatagaat ggatacccgc ttaatatcca aattactaca taaactatcg
     4261 agggcaatgg catagatatc ttcaactgcg ttaatttgac ttaaagcaga agtaatttga
     4321 tacaaactat tcagttgttt gttttgctgc gtgagatatt cttctacttt tttgatctcg
     4381 gtaatatccc gcgagatcga accaagcctg caccccttat ctgtacggat cgtaaatatt
     4441 ctttgttgga tgattaatac actaccgtcc gcccgctgaa atgggacttc ctgcgtaaat
     4501 tggaaataag aagtagcgcc aattttgaga gcttgttgaa tccgatttcg aagagtaaag
     4561 ttttcatcat ctgcttttgt actgtctggc atgagttcat aagcaagctc ccacaggaat
     4621 ttacctaaga cattctctcg ctttttccct gtaagttgtt caaatgcttc attgaattca
     4681 agaatgcgtc cctcttcatc cgatagtgca atgccatctg cagatttatc aattacgcca
     4741 tgaaattttc tttcactttc tttcaaggca ctctcagact gtttccgctc tgtaatatcc
     4801 cgtccattga taatgacgcc cataatcgca gggtcatcca tcaagtagtg agtgacaatt
     4861 tcaaaatagc gccaattgtt ttctgcattc aataagcgat attcgccacg atgtacaaat
     4921 cctggagaat ttaagccatc gacgaacgtc ttaagaatct cagataaatc atcaggatgg
     4981 atcaagttaa atgaggatgt accgatcaat ttatcttgcc cataccccaa caaacgtgca
     5041 acggatgggc tttcatacgt cacaaccccg ctcccatcaa tcaccagaat cccatccgca
     5101 gtattctcta tcagagcgcg atagtagcgt tcactctcat atagagctct ttccatctcc
     5161 ttacgttcgg taatatctct gaagttaaca actattgcct ctactgcagg ttctgcaaac
     5221 tgattgctaa atatactttc cacccaatga taagaaccat cttgatgtcg gtaacgatac
     5281 tgaaccgtac ggggagaaga ggaatctcct accagattgt tcatcgttgc aagaacggcg
     5341 cctatatcat ctgggtggat agaatccata gggttttcat tg
//
LOCUS       DAOQNJ010000116         5342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9148_length_5342_cov_8.516172, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000116 DAOQNJ010000000
VERSION     DAOQNJ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9148_length_5342_cov_8.516172"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLG52_04785"
     CDS             complement(<1..392)
                     /locus_tag="PLG52_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40792.1"
                     /translation="MSFAQAIMEALESLNGNKMRSGLTVLGIVIGVAAVIAMLAVGNG
                     AQASITGSISSIGTNLLFIFGGAPSQDGPPGSGPRTSGNNERPLTLGDSNALLDTFAA
                     PSVEMVAPAIQGNALLTFSSEKTSTSISG"
     gene            complement(389..1087)
                     /locus_tag="PLG52_04790"
     CDS             complement(389..1087)
                     /locus_tag="PLG52_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40793.1"
                     /translation="MDWVIEARDLKKTYVMGEIEVQALRGVSFTVTRGEVVSIMGPSG
                     SGKSTMMNTLGCLDRPSSGEYILDGESVAQMNDDQLATVRNRKIGFVFQSFNLLSRLS
                     AVGNVELPLRYAGITQGRREKAQAALEAVGLKDRMFHRPYELSGGQQQRVAVARALVN
                     NPAIIMADEPTGNLDSKVGKEIMTLLLNLNKDLGTTLVIVTHDPKIAEQTQRVIRLKD
                     GELDSIDINSGGAQ"
     gene            complement(1089..2354)
                     /locus_tag="PLG52_04795"
     CDS             complement(1089..2354)
                     /locus_tag="PLG52_04795"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB40794.1"
                     /translation="MRQFFSRFSRRTWIIIAVVAVVLICVGVSIFRPKNEVAVFETVA
                     VERGDLAAYVGATGSVRARQTATLTWQTSGIVGEVNAVIGDRVERGFVLSGLDKATLS
                     QSIILAEADLVSAQKALDDLLQSNTSLLEAEKAVDKAEESYQKAYNWRMDLNGKVDIK
                     EFYYDQFGELKVKQYKGYASAETIADADRDLALAESKLEDARREYERLQKGPDSDEVA
                     AARARVAAAEATLNMAKIIAPFDGTITQSNPVVGDQVNPGTVGFRIDDLSSLLVDVQV
                     SEVDINNVAVGQDATLSFDAILGKSYNGKVVEVGQAGDTVQGVVSFTVTVELTDADEQ
                     VKPGMTTAVNIITEEVKNTLLIPNRAVRLVNGERVVYLEKDGQATPVTITLGASSDTM
                     SVLTSGDIKEGDLIILNPPSANGGPFGGG"
     gene            2676..4118
                     /locus_tag="PLG52_04800"
     CDS             2676..4118
                     /locus_tag="PLG52_04800"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB40795.1"
                     /translation="MQAVPSEFLAIDRKERANRPFFDLDLRAADDRKCDFGAVVIEFD
                     PERAMVEAARCIHCPDPAPCMVACPTANDIPSAMWLIEQGKFVEAAGIYHKTSSLPEV
                     CGRVCPHESLCQGSCVLNKHHTPVLCGELEAFTVDYERRTQGYLIPLTIPSGKKVAVI
                     GAGPAGLGCADILLQKGHEVTIFDAKPAPGGLLVYGIPNFKLAKDVWGEKWEEFERAG
                     AKFVPNTYIGKDKTIDQLFAEGFDAVFIGVGSEIDAKMEEAPGTDLPGVYEATDFLIR
                     GNIPEDQLPSDMKKPLEIGKRVVVVGGGDTASDCLRTAIRLGAEEVTCLYRRTEKEMP
                     GGKKDRKMARDEGAKYRFLTQPVKFIAGADGKLAAVECIEMKLGEPDAKGRRKPVPVE
                     GSNFSVAADTAILALGYWPDPVIGKSTPDLETYNWGLVSVKDKKTGATSREGVFAGGD
                     CATGPDLVVTAMVGGRKAGFAIDEYLKNKK"
     gene            4285..>5342
                     /locus_tag="PLG52_04805"
     CDS             4285..>5342
                     /locus_tag="PLG52_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40796.1"
                     /translation="MRWNKVSLSFLALFLFFLLFFSLYLPLRPVGQILDRGWTAEYKG
                     VQTSNVQIPYIQRSSEEADILTLTTTLTDIQGNSLIFRPGGYAFSVFLNGQQIAGIGD
                     LEEPTANVWNSIQLIPLTNLNSGSNTIQIKLSSANRSVGLGNPPYIDNLKTSITRVAF
                     INWFANDFLILNQGAGFLVGALLIAIARSRRLLFSTEFYMGMAVILAAIYVFDFDTRP
                     STGSLEILLLVRKICLTSGYLGALLFVCSLELYYQGKLKISQIIAIPTFLAVIILSVQ
                     PSQYQFSITLIYSNIVLLINLSVACFFIFQNSKGKDWLIVPALLLTLSILESILVMAF
                     VRPWPFMTQYIVLFSAVF"
BASE COUNT         1362 a         1259 c         1271 g         1450 t
ORIGIN      
        1 cccgaaatag aggtgcttgt tttttcacta gagaatgtga gcaaagcatt gccttgaatc
       61 gctggtgcta ccatttctac ggaaggagcc gcaaaagtat ctaacaaggc attagagtcg
      121 cccaaagtca atggacgctc gttgttacca ctggtgcgtg gacctgaccc aggaggtcca
      181 tcttggctgg gcgcaccacc gaaaatgaag agtaagttcg taccaatgct agaaattgaa
      241 ccggtgatgg acgcttgtgc gccatttcca acagctaaca tggcaatcac cgccgccaca
      301 ccgatgacaa ttcccaacac agtaagccct gaacgcattt tgttgccatt caggctttcg
      361 agggcttcca tgattgcttg tgcaaaactc attgtgcgcc tccggagttt atatcgatgg
      421 aatctaattc gccatctttt aagcggatta cgcgctgagt ttgctcggca atcttcgggt
      481 catgcgtgac gatgacaagg gtggtcccga gatctttatt caagttaagc aaaagagtca
      541 taatctcttt accgactttt gaatccaagt taccagtcgg ctcatctgcc atgatgatgg
      601 cagggttgtt gacgagtgcg cgggcaactg ctacacgctg ttgctgaccg cctgaaagtt
      661 cataggggcg gtggaacata cgatctttaa gacccacggc ttcgagagca gcttgtgcct
      721 tttcgcggcg tccttgtgtg atgccggcat agcgtaaagg cagttctaca tttccgacag
      781 cagagaggcg cgaaagcaga ttaaagcttt gaaagacaaa gccaattttg cgattacgaa
      841 ccgttgccaa ttgatcgtca ttcatctgcg caacagactc gccgtccaaa atgtattcac
      901 ctgatgatgg gcgatctaaa cagccaagag tgttcatcat ggtcgattta ccggagccgg
      961 aaggacccat gatggaaacc acctctccgc gagtcacagt aaatgaaact ccgcgtaagg
     1021 cttgcacttc gatctcaccc atgacgtagg tcttttttaa gtcgcgtgct tcaatgaccc
     1081 agtccatgct agcctccacc gaagggacct ccattggctg agggcgggtt gaggatgatc
     1141 aaatcgcctt ctttaatatc accgctagtg agcacactca ttgtgtctga ggaagcgcct
     1201 aaggtgatgg tgacgggtgt cgcttgccca tctttttcaa gatagaccac gcgttcgcca
     1261 ttcactaaac ggacagcgcg gttcggaatg agaagcgtgt ttttcacttc ttcggtgatg
     1321 atgtttacag cggtggtcat accgggcttg acttgttcat ctgcatcggt caattcaaca
     1381 gtgaccgtaa agctaacaac gccttgaacc gtatcgccag cctgacctac ttcaacgact
     1441 ttgccattat aggattttcc aagaatcgca tcaaacgaaa gggtggcatc ttgcccaaca
     1501 gcaacattgt taatatccac ttcagagact tgtacatcga ccaacaagct ggagaggtca
     1561 tcaatgcgga agcctacagt gccggggttt acctgatcac ctaccactgg attagattgg
     1621 gtaatcgtgc catcaaaagg tgcgatgatc tttgccatat tcaatgtggc ttcagcggca
     1681 gcaacacggg cacgagccgc ggctacttca tcagagtccg gtcccttttg gaggcgttca
     1741 tattcacggc gagcatcttc cagttttgat tcagccaagg caaggtcacg gtcggcatct
     1801 gcaatggttt cagcactggc atagcccttg tattgcttga ccttcaattc gccaaattgg
     1861 tcgtaataaa attctttaat atctactttg ccattgagat ccatgcgcca gttataggct
     1921 ttttggtagg attcttcggc tttatcaaca gctttttcag cttctagcaa agacgtattc
     1981 gactgaagta aatcatctaa ggctttttgt gcgctgacca ggtcagcttc ggcgaggata
     2041 atgctctggc tgagagtcgc tttgtcgagt ccggacagga caaatccacg ttcgacgcgg
     2101 tctccaatca cagcattcac ttcccctaca atgccactcg tctgccaggt aagggtggca
     2161 gtttgtcggg cacgaacact gccagtggcg cctacatacg ctgccaagtc accgcgctct
     2221 actgcaacag tttcaaaaac ggctacttcg ttcttcgggc ggaaaataga aacaccgacg
     2281 caaattagga ccaccgctac aaccgcgata atgatccatg ttcgtcggga aaatctagaa
     2341 aaaaattgac gcattcggga gttcctccaa ttaggttaca gtaagcgtgc agtgctgttt
     2401 tcttacattg atatatcaca ctctacgcaa aagattgtgg aaagtttgtg cagattttaa
     2461 ctaatcgatt ctatctcttt atcttgtaat ttgacagtca aaaaagcgac atttcaaaat
     2521 tacaagcgag cgatacctat taatcaaaga ctcataaatt tatgacgatt gtctttgggt
     2581 ttggtgacat ttaaacctag ttctatccga tttatttggt tattattcgg gaaaccgctt
     2641 tatttgtatc tttctcccct ttggaggcat tctgcatgca ggcagtaccc agcgaatttt
     2701 tggcaattga ccgcaaggaa cgggcgaacc gtcccttttt tgatttagat ttgagagccg
     2761 cagacgatcg gaagtgtgac tttggggctg tagtgatcga atttgacccg gagcgcgcca
     2821 tggtggaagc ggctcgctgc atccactgcc cagacccggc tccgtgcatg gtggcgtgcc
     2881 ccacagccaa tgatattccc tctgccatgt ggctaattga gcagggcaag tttgtagagg
     2941 cggctggtat ttatcacaag accagctccc taccagaagt ttgtgggcgc gtttgcccgc
     3001 acgagtcatt atgccaaggg tcttgcgtgc tgaataagca tcatacgccc gtactgtgcg
     3061 gtgagcttga agcctttacg gtagattatg aacgccgtac acaggggtat ctcattccgt
     3121 tgacaatccc ttctggcaaa aaagtagcag tgatcggggc aggacctgcc ggtttgggtt
     3181 gtgccgatat tttgctacaa aagggacacg aggtaacaat tttcgatgcc aagccagccc
     3241 ccggtggctt gctggtgtat ggcattccaa actttaaact tgctaaagat gtatggggcg
     3301 agaagtggga agagtttgaa cgcgccggtg caaagttcgt ccccaatact tatattggaa
     3361 aagacaaaac catcgatcag ttatttgccg aaggttttga cgcggtcttt attggggtcg
     3421 gttctgaaat tgacgcaaag atggaagaag cgcccggcac cgacctgccc ggagtgtatg
     3481 aagcgacaga ttttctcatt cgcgggaata ttccagaaga tcaacttccc agtgatatga
     3541 agaagccgct cgaaatcggt aaacgggtgg tggtggttgg tggcggggat acagcttcgg
     3601 actgtctccg aaccgcgatt cgtcttggcg ctgaagaagt aacctgtctg tatcgccgta
     3661 ctgaaaaaga aatgcccggc ggtaagaaag atcgcaaaat ggcacgagat gaaggggcaa
     3721 agtatcgctt cctcacccaa cctgtgaagt tcattgccgg tgcggatgga aaattagccg
     3781 cagtggaatg cattgaaatg aaacttggcg agcccgatgc aaaaggacgc cgcaaacctg
     3841 taccggtaga aggttccaac tttagtgtcg cagcggacac cgccattctg gcgctcggct
     3901 actggccgga ccccgtaatt ggcaaatcca ccccagacct tgaaacctac aattggggct
     3961 tggtctccgt aaaagacaaa aagaccggcg cgaccagccg tgaaggcgtg tttgctggtg
     4021 gcgactgcgc aactggacca gacctcgttg taactgccat ggttggcgga cgcaaagccg
     4081 ggtttgccat tgatgagtac ttgaagaaca agaaatagcg aattacctgt tgagggcagc
     4141 aggtcgttag cacggcagag tcaccagctc tgccgtgcgc cttaataata tataaaagcg
     4201 agagggcgta taattttagg caataaaaag agtatgtatt ccaggcaagt ctaagacaaa
     4261 tctcaaattt ggaaattgac aataatgcgt tggaacaaag tctctctatc ttttctagcc
     4321 ctatttcttt tttttcttct tttcttttcg ctctatctgc cattgcgccc ggttgggcaa
     4381 attctagacc ggggttggac tgccgaatac aaaggcgttc aaacttctaa tgttcaaatc
     4441 ccatacattc aacgtagtag tgaagaagct gatattctca ccctgactac aactctgacc
     4501 gatattcaag gaaatagcct tattttccgc cctgggggat atgctttttc tgttttctta
     4561 aatggtcaac agatcgcagg aattggagac cttgaagaac cgaccgccaa tgtatggaac
     4621 tccatacagc tcattccact tacaaactta aattctggct ccaatactat acaaatcaaa
     4681 ctcagtagtg ccaatcggtc cgttggttta ggcaatccgc cttatataga taatctaaaa
     4741 actagtatta cgagagtcgc atttattaat tggttcgcaa acgacttctt aattctcaat
     4801 caaggagcag gctttttggt tggagcgctc ttgattgcaa ttgcccggtc acgtcgtttg
     4861 ctttttagca ccgaattcta tatgggcatg gcagtaattc tggcagctat ctatgtattt
     4921 gattttgata cgcgtccctc aactggcagt ttagaaattt tattacttgt tcgaaagatt
     4981 tgcctaacaa gtggatattt aggtgcattg ctctttgttt gcagtttgga actttactat
     5041 caaggaaaac taaagattag ccagatcatc gctattccca cttttctggc ggtcatcata
     5101 ttgtctgttc agcccagcca gtatcaattc tcgattactc tgatttactc caatatcgtt
     5161 ttattaatca atctttctgt agcatgtttc tttatcttcc aaaatagcaa aggaaaagat
     5221 tggctcattg tgccagcgct gctcctaact ctttccattc ttgagtctat tttggttatg
     5281 gcatttgtca gaccatggcc ctttatgacc caatacatcg ttcttttcag cgctgtcttt
     5341 tt
//
LOCUS       DAOQNJ010000117         5297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9299_length_5297_cov_8.896223, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000117 DAOQNJ010000000
VERSION     DAOQNJ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9299_length_5297_cov_8.896223"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..630)
                     /locus_tag="PLG52_04810"
     CDS             complement(352..630)
                     /locus_tag="PLG52_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40797.1"
                     /translation="MSEERAKPKAFATLCGRIFRDSDPQYFPFAEYKGKKILLCTEAC
                     LGAFLADPDIFCKVHRNSDKTAARMKKEIEHLATLWSQYASERPTEEK"
     gene            complement(627..2345)
                     /locus_tag="PLG52_04815"
     CDS             complement(627..2345)
                     /locus_tag="PLG52_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNB40798.1"
                     /translation="MTSLKWWQTAVFYQIYPRSFADGNGDGIGDFKGITEKLDYLVSL
                     GVDALWLSPHFPSPNWDCGYDISDYTNVAPEYGTLEDFKTFLKESHKRNIRVILDLVL
                     NHTSDEHPWFLESKSSRDNPKADWYVWVDTPPNNWQSCFDGDAWTYVPERKQYYYHYF
                     MKQQPDLNWHNPEVKAAMWDAVRFWLDLGVDGYRLDAIGTIYEDANLTPHTVPMTLAE
                     LRRFSELAETEEDKKLREKYWVEMFKNQWGQPGVHELMKELRSILNEYDGDRMLVGED
                     DNIDFMGDGEDELHLVFNFPLANVNRITPEHIRKNQTERIARLNQLAKGGWACNTLGN
                     HDRSRIYTFYGDKLHDAELARLNAALTLTLRGTPFLYNGEEIGMTDYLLPHISQVKDT
                     MGSWYYHTIVTEMGVDPEEAMFRTAEMTRDKNRTPMHWSNKANAGFSPEGVETWLPIN
                     PNYKEGINVFEQEQNPSSILNYYKHLLKVRKASPALVEGDYLPLHEDATDYFAFIRKS
                     DKQTVLVVLNYSDKRLRLNFSSDQDINGKNLHTLFTSAVRSPADHKADNLTIAPFEVF
                     IAEVTA"
     gene            complement(2398..2664)
                     /locus_tag="PLG52_04820"
     CDS             complement(2398..2664)
                     /locus_tag="PLG52_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNB40799.1"
                     /translation="MIPDRVITLAELKRNTGERGTRMWIAHLGIVYDVTECPKWRAGM
                     HEFLHFPGQDLSSEMEDAPHEDEVFKHDCVKIIGKLEEQNVSTS"
     gene            complement(2661..3392)
                     /locus_tag="PLG52_04825"
     CDS             complement(2661..3392)
                     /locus_tag="PLG52_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40800.1"
                     /translation="MTNQTPIINFSPKKILIFFFSLVALLVALSIWGQHMRFFGVGDI
                     RGPIHEMFIDIMMTSFYLDYESNVPTFINALMLFIPALLLLAIGLWKSNIKDKYRFQW
                     NALAFIFFLLSIDEIASFHERLIKPMRAAVGSHGVFFFAWIIPGMAAIALFGFAFLTF
                     FLHLDKKFKLLFFFSLAVYISGVIGGEMVSGYFAANLGQKNFTYAVVASLEESVEMFG
                     CGLIIYSLLEYIKHYLPEGLTFKGS"
     gene            complement(3443..4480)
                     /locus_tag="PLG52_04830"
     CDS             complement(3443..4480)
                     /locus_tag="PLG52_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNB40801.1"
                     /translation="MKAVQFTGVNQPLQMQEIPIPEIGERDILVKIKAAGVCHSDAHY
                     RAGISQVRPVPLTLGHEVAGVVEKIGANVTNVKIGDRVALHYNLSCGDCYYCSTGNDQ
                     FCEKVLMLGHYTNGGYAEYIAVPARNAVHLPEEIPFEQGATLMCASATAFHALRKSRI
                     QGGETVAIFGAGGLGQSAIQLAKAFGALEVYAVDINESKLDLAKQHGAIPVNAQKVDA
                     VAEIKRLTYNKGVNVAIEMIGLPQTMKQALQVAGVMGRVVIVGVSNKMLEVQTYTELL
                     GNEVELIGSNDHHLQELPLLVDLARRNVLDTSKVVSKIIPLDADAINAALDELERFGS
                     DVRTVIVPASS"
     gene            complement(4484..4948)
                     /locus_tag="PLG52_04835"
     CDS             complement(4484..4948)
                     /locus_tag="PLG52_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB40802.1"
                     /translation="MIQLTPIATVKNTRIEIEDDNWGNVVSVIELDTSIPEEALLEIE
                     SFSHAEVIYYFHLVPDAKIETGSRHPRNNTAWPKVGILSQRGKNRPNRLGATIVKIVK
                     REGHQLHVQGLDAIDGTPVLDIKPVMREFLPREEIRQPEWATELMKNYWAKE"
     gene            complement(4950..>5297)
                     /locus_tag="PLG52_04840"
     CDS             complement(4950..>5297)
                     /locus_tag="PLG52_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40803.1"
                     /translation="GSANIETQITLMNSRVIQLLTQDKNLWQWAGDQLYVDLDLSEEN
                     LPAGTRLSIGTAVIEVTATPHTGCKKFSARFGLDALKFISASENKSLRLRGINARIIQ
                     PGEFKTGDLVRKI"
BASE COUNT         1395 a         1172 c         1230 g         1500 t
ORIGIN      
        1 cctgtgggtt ggtcagcccc gtcagcagta cggctcgcgg acggctgtac gccagtacat
       61 cgctcattaa gtctgcacag caaccgccat gaatgtttac atcagtattc agctcggggg
      121 ttagtaattc accttctgca atcacaataa tgtctttcaa attcatattc atatataaag
      181 ggatggtcct ttcacctcat aataaaactc tctttaaaat tattccctac ttagtctgtt
      241 taggtaagtt tcttttctca tgctcggtag gtgactattg acctgttaag tgataaattt
      301 cacaaatata taacaaataa aaaaacgctc tttagagcgt ttttttattt gttacttttc
      361 ttcagtaggt ctttcagaag cgtattggct ccataaagta gctagatgtt cgatttcttt
      421 tttcatgcga gctgcagttt tatctgagtt gcggtgaact ttacagaaaa tatctgggtc
      481 tgcgagaaag gcaccaaggc aagcctcggt gcaaagcaaa attttcttgc ctttgtattc
      541 agcaaaagga aaatactgtg ggtcactatc tcggaagata cgtccgcaca gggtggcgaa
      601 tgcctttggt ttcgcccgtt cttcactcat gcagtgactt ccgcaatgaa gacttcaaat
      661 ggcgcaatgg tgaggttatc tgctttatgg tcagcagggg agcggacagc actggtgaac
      721 aaggtatgta agttctttcc atttatatct tgatcacttg agaaattcaa gcgtaagcgt
      781 ttatcggaat aattcaatac aaccaatacc gtctgtttgt ctgactttct aatgaatgca
      841 aagtagtccg ttgcatcttc atgaagcgga agataatctc cttctaccaa tgcaggtgac
      901 gccttacgga ccttgagcaa gtgcttgtag taattcaaga tcgagcttgg gttttgctcc
      961 tgctcaaaaa cattgatgcc ctctttataa tttgggttga tcggcagcca agtttctaca
     1021 ccttctggag aaaaaccagc atttgcttta tttgaccaat gcatgggggt gcggttcttg
     1081 tcgcgggtca tttctgctgt gcggaacatg gcttcttctg ggtccacgcc catttcggtg
     1141 acgatggtat ggtaatacca ggagcccatt gtgtctttaa cctgcgagat atgcggcaga
     1201 agatagtcgg tcatgccgat ttcttcacca ttatagagga aaggggtgcc gcgtaaagta
     1261 agagtaaggg cagcattaag gcgagctaat tcggcgtcgt gcagcttatc gccataaaac
     1321 gtgtaaatgc gagaacggtc gtggttaccc aatgtattgc aagcccagcc acctttggca
     1381 agttgattta ggcgtgcaat ccgttcggtt tgattttttc gaatgtgttc cggagtaatg
     1441 cggtttacat ttgccaaggg gaagttaaag acaagatgca actcatcttc gccgtcgccc
     1501 ataaaatcaa tgttgtcatc ttcgccaacc agcattcggt cgccgtcata ttcgttgaga
     1561 atgctacgca gttctttcat caactcgtga acgccgggtt gaccccattg attcttgaac
     1621 atctcgaccc aatatttttc tctaagcttc ttatcttctt cggtttctgc aagctctgag
     1681 aagcggcgca attcagcaag agtcatggga acggtatgag gcgttaggtt ggcatcttca
     1741 taaatggtgc cgatggcgtc aagacgatac ccatccacac caagatccag ccaaaagcgg
     1801 accgcgtccc acatagcggc tttgacttct ggattgtgcc agttcaaatc tggctgttgt
     1861 ttcataaagt agtgatagta atactgtttt cgttcgggaa cataggtcca tgcatcgcca
     1921 tcgaagcagg attgccagtt gttgggcgga gtatctaccc aaacgtacca atccgctttg
     1981 gggttgtcgc ggctggactt ggattccagg aaccaagggt gttcgtcaga agtgtgattc
     2041 aagacaaggt ccagaatgac gcggatgttt cgcttgtgag actcttttag gaaggtcttg
     2101 aaatcttcga gggttccata ttctggggca acattggtgt aatctgaaat gtcgtaaccg
     2161 caatcccaat tgggggaggg aaaatgcggc gaaagccaaa gcgcatccac accgagactg
     2221 acgaggtaat caagtttttc ggtaatacct ttaaagtcac cgatgccatc tccattgcca
     2281 tcagcaaacg aacgcggata gatttgataa aaaacagcag tttgccacca tttgagtgat
     2341 gtcattgtag atgtaattcc ttttgcattg agagtttgaa agtttatagg caagaaatta
     2401 agaagttgaa acgttctgct cttccaactt cccaattatt tttacgcagt catgtttgaa
     2461 tacttcatct tcatggggtg catcttccat ttcgctgctc agatcctgac ctggaaaatg
     2521 cagaaactca tgcattccgg ctctccactt ggggcattcg gtcacatcgt agacgatgcc
     2581 aagatgggcg atccacatgc gcgtcccacg ttcgcctgta ttgcgtttta gctctgcgag
     2641 agtgataaca cggtctggaa tcatgagcct ttgaacgtga gcccttcggg aagatagtgc
     2701 ttgatgtatt caagcaggga ataaatgatg agtccacagc cgaacatttc aacagattct
     2761 tctaaactgg cgaccacagc gtaggtgaaa tttttctgcc cgaggttggc agcaaaatat
     2821 ccactaacca tttctccgcc gattacgccg ctaatatata ctgccagcga gaagaaaaag
     2881 agcaatttga actttttatc gaggtgtaag aaaaaggtga gaaaagcaaa gccgaaaagt
     2941 gcaatagcag ccatgccggg aataatccaa gcaaaaaaga aaacgccgtg cgaaccgact
     3001 gcagcacgca tcggtttgat aaggcgttca tgaaatgaag caatttcatc tatcgaaagc
     3061 aagaaaaaga tgaaagcaag agcattccat tgaaaacgat atttatcttt gatgttgctt
     3121 ttccacaagc caatggcaag aagtagaagt gctggaatga aaagcattag ggcattaatg
     3181 aaggtcggca cattgctttc atagtccaga taaaaggatg tcatcatgat gtcgatgaac
     3241 atttcgtgta tgggaccgcg aatatcgcca acgccaaaga agcgcatgtg ttgtccccaa
     3301 atgctgagtg ccactaacaa cgctacaaga gagaaaaaga aaatcagaat tttcttgggg
     3361 ctgaaattga tgataggagt ttggtttgtc atagatacct gtgagcgttg attatacgtc
     3421 gaaacgctgg acagttgtcg gcttaggaag aggcgggaac aatgactgtg cgcacgtcac
     3481 tgccaaagcg ttccaactca tcgagcgcgg cgttaatggc atccgcatct aacggaatga
     3541 tcttgctgac tacttttgat gtgtctaata catttcgacg ggcaaggtcg acaagcaagg
     3601 gaagttcttg caagtgatgg tcgttggacc cgatgagttc cacttcatta ccaagcagtt
     3661 ctgtgtaggt ttgcacttcc agcattttgt tcgaaacccc cacaatgacc acgcgtccca
     3721 ttacccctgc aacttggagc gcctgtttca tggtctgtgg aagtccaatc atctcaatgg
     3781 caacattgac acctttgtta taagtaaggc gtttgatctc tgccactgca tctacttttt
     3841 gggcattgac gggaatcgcg ccatgttgtt tagcaaggtc aagtttcgac tcattgatat
     3901 cgacggcata tacttcgaga gcgccaaacg cttttgccaa ttgaatagca gactgaccga
     3961 gtccgcctgc gccgaagatt gctaccgttt ccccgccttg aatgcggctc ttgcggagtg
     4021 catgaaaggc agtggcactg gcacacatca aagttgcacc ttgttcaaaa ggaatttctt
     4081 ctggtaagtg aacggcattg cgtgctggca cggcaatata ttctgcgtaa ccgccgttgg
     4141 tgtaatgccc aagcatcaaa actttttcac aaaattgatc gttgcctgtt gaacagtaat
     4201 agcaatcgcc acacgaaagg ttgtagtgaa gtgcaactcg atcaccaatt tttacattgg
     4261 ttacgtttgc accgattttt tcaaccacgc ccgctacttc atgccctaag gtaagaggta
     4321 cagggcgcac ttgtgaaatg ccagcacgat aatgagcatc cgaatgacaa acccccgctg
     4381 ctttgatctt tactaaaata tcccgctcgc caatttctgg gattggaatc tcttgcattt
     4441 gtaacggttg gttgacacca gtaaattgaa ctgctttcat gtattactcc tttgcccaat
     4501 aatttttcat taactctgta gcccattcag gctgacgaat ttcttctcgc ggtagaaatt
     4561 cacgcatgac aggttttata tccaagacag gagttccgtc gatggcgtcc aatccctgaa
     4621 catgtagttg atgaccttct cgcttcacta ttttaacgat agttgctcca agcctattgg
     4681 gcctgttctt accccgttgg ctgagaatcc ctactttggg ccaagccgtg ttgttgcgcg
     4741 gatgacgtga cccggtttcg atttttgcat ctggaaccaa atgaaaatag taaatgactt
     4801 ctgcgtgtga aaaactttcg atttccagca acgcttcttc gggtatagat gtgtccaatt
     4861 caatgacaga gacgacatta ccccagttat catcttcgat ttcgatgcgc gtatttttta
     4921 cggtagcaat aggagttaat tggatcatat tagattttcc gtaccaagtc accagttttg
     4981 aactcgccgg gttgaattat gcgagcattg attccgcgca agcgcagcga tttgttttcg
     5041 gaggcactga taaattttaa agcatccagc ccgaagcgag cagagaattt cttgcaacct
     5101 gtgtggggcg tagccgtcac ttctatgact gctgtgccta ttgaaagccg agtccctgca
     5161 ggtaggtttt cttcactaag gtcaaggtct acatagagtt ggtcacctgc ccattgccaa
     5221 agatttttat cttgggtcaa caattggatc acgcgtgaat tcattagagt aatttgtgtc
     5281 tctatgttgg cagaccc
//
LOCUS       DAOQNJ010000118         5297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9300_length_5297_cov_7.268218, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000118 DAOQNJ010000000
VERSION     DAOQNJ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9300_length_5297_cov_7.268218"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PLG52_04845"
     CDS             <1..602
                     /locus_tag="PLG52_04845"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNB40804.1"
                     /translation="MPLRYLALGDSYTIGESVEEAERFPNQLASLLANEGVTVDVKII
                     ARTGWTTDELWRGIQAEVVEPPYDMVSLLIGVNNQYRGRGLEEYREGFIFLLNKAIEY
                     AGGDVKRVVVFSIPDWGVTPFAYGRDTTQIANEIDAFNKVNLEESTKAGAHYVDVTPS
                     SRLALNDKTLIASDGLHPSGKMYAEWAQSALPIALNILK"
     gene            634..1038
                     /gene="mce"
                     /locus_tag="PLG52_04850"
     CDS             634..1038
                     /gene="mce"
                     /locus_tag="PLG52_04850"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNB40805.1"
                     /translation="MPQIKAINHVAVVVEDMEKSLAFWRDALGIKLHELRDVPAEKSQ
                     VAFLPLAGAEVELVMPTTDDSGIAKYLAKRGQGMHHLCLEVDDIEGMMAQLKAKNIRL
                     INEEPRTAADGKKYAFIHPESTGGVLVELYQI"
     gene            1039..2502
                     /gene="tilS"
                     /locus_tag="PLG52_04855"
     CDS             1039..2502
                     /gene="tilS"
                     /locus_tag="PLG52_04855"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNB40806.1"
                     /translation="MSSNVETIMVEECGLVKDRPVIVGVSGGPDSLCLMETLRRAGYH
                     LIIGYFDHKLRPESALDARMVEKAAVRLGLLCILGGMDVRSYAEEKKLSIEEAARTLR
                     YRFLFELARKYKAQAVAVGHTADDQVETVLMHFLRGSGISGLKGMSYRSVIKSFDPDI
                     PVVRPLLDMWREETVLFCAVNGLRPSYDSSNESLNFQRNRIRHLLIPSLETYNPKFRE
                     TVLRMSHSLKGDYAFVMETLEHAWGNVVVSANDEIITFDVLRLSSMPIGLQRNLIRHA
                     MQTLLPGVDIGYQTLKRASDFINASSATVPRLDLKAGLRMFREAGLIHICAPYAELPF
                     DRWPQMVDDKSVPLRLGGQASLAGGWKVICESWRLPTLAREQAEENEDQFQVWLDADE
                     LPEEFLLRARRQGDHFSPLGMGGHSQKLSDFFVNEKLPHRARDRWPLLCVGDEIIWVP
                     GFRPAHRYRLKETTHNVIYFSVQHPPEKIAPDENPHP"
     gene            complement(2522..3421)
                     /locus_tag="PLG52_04860"
     CDS             complement(2522..3421)
                     /locus_tag="PLG52_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNB40807.1"
                     /translation="MTKNSAPAHISAKTLLPAAINGWEMFLLDQGRSPNTVKAFLSDV
                     RLLTQFLPPDQAIGTVTTDDLNRFFEWMEKGRGVACSPKTLSRRITSVKSFFRWLHQY
                     GTLSIDPAEKVLQRSAISPLPQVLTDEEYEAVLLAADRQRRAKKPDARPYTLLSLLLQ
                     TAIKKSECLGIHVNHIELAGKNGPFLFVRYASPANRYKERKIELTEDWVEAYNEYLTQ
                     YSPVDQAFPWSPRRLEYLLEDISEEAGLEKHLSFDMCRWTSALRDYQNGIEPDKIRQK
                     LGVSKIQWRELFIKLKQLNGEVK"
     gene            complement(3546..4742)
                     /locus_tag="PLG52_04865"
     CDS             complement(3546..4742)
                     /locus_tag="PLG52_04865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNB40808.1"
                     /translation="MQNEFKEDAPTPSGKPTSQRAGGTSNGPSLFTRLINSLVNMGLG
                     EPLIKLGVNLFSVVAIVLVVFMSRSFYRQAQDMPVAPNQDSGPDIEAAPAESLAVLDI
                     PVTDGINRAAQLHTTIPSRPRNEVSTYVVQDGDTVFGIAEKFGLKPQTILWGNYNTLL
                     DDPHSLKPGQELTILPVDGVYWEWLGGIPFGEWAKFYGVNAADIIEYPGNNIDPTTVG
                     DYENANIKAGTWLIIPNGKRDFVSWSAPLGVTRENPASARVLGAGACDPVSGGAVGYG
                     SFIYPTNKHYLSGFDWSPQTNHNGLDFAGNTGEAVYASDAGVIVYAGWNDYGYGNMIM
                     VDHGNGFQSLYAHLSALNVGCGQSVGQGEVIGAVGSTGRSSGAHLHFEIMAGVKVNPW
                     DYLPPP"
     gene            complement(4807..>5297)
                     /locus_tag="PLG52_04870"
     CDS             complement(4807..>5297)
                     /locus_tag="PLG52_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40809.1"
                     /translation="PLLTSEAAIATRTSNLYTAQGIGAVTAAFMMAYFATANKNSTLL
                     WGQILFIFPIIVLGLTTNASLAFFLLIFIGWGTVVQLISMNTMIQMRVPNDLRGRVFS
                     IYFWGLQGVAPFGSILIGWIAQTRDVQFAIVTGGIACLVGISVVRLLFFRKNEPTEQI
                     SY"
BASE COUNT         1352 a         1217 c         1355 g         1373 t
ORIGIN      
        1 tcatgcccct gcgatacttg gctttaggtg actcttatac catcggtgaa agtgtggaag
       61 aagcagagcg cttcccaaat caacttgcat cattgcttgc aaatgaaggt gtgacggttg
      121 atgtgaaaat catcgctcgc accggctgga cgacagatga gctttggcgc ggcattcaag
      181 ccgaagtggt ggagccgccc tatgatatgg tctctttact gattggcgta aataatcagt
      241 atcgtggcag agggcttgaa gaatatcgcg aagggttcat atttttgcta aataaagcca
      301 tcgaatatgc tggcggagat gtaaaacggg tcgttgtctt ctctattcca gattggggtg
      361 tcactccgtt cgcatatggc agagacacca cccagatcgc aaatgaaatc gatgctttta
      421 ataaagttaa cctcgaagag tccaccaaag caggtgctca ttatgtagat gtaaccccta
      481 gttcccgctt agcgctcaac gataaaaccc tgattgcatc cgatggattg catccctctg
      541 gcaaaatgta tgctgagtgg gcacaatctg cgttaccgat tgcgttgaat atcttgaagt
      601 agaatctttt cataaatcaa aaacggagaa tcaatgcctc agatcaaagc tatcaatcat
      661 gttgccgttg ttgtcgagga tatggaaaag tcgcttgcgt tttggcgcga tgccctggga
      721 attaaactac atgaattaag agacgtaccc gccgagaaat ctcaagtggc gtttttgcct
      781 ttggctggcg ctgaggtgga attggtgatg cccaccaccg acgactctgg cattgccaag
      841 tatcttgcca aacgtgggca agggatgcat catttatgcc ttgaagtgga tgatattgag
      901 ggaatgatgg cacaattgaa agcgaaaaac attcggctca tcaatgaaga accgcgcacc
      961 gcagcagacg ggaagaagta tgctttcatc caccctgagt cgacgggcgg cgttttagtg
     1021 gagttgtatc aaatctaaat gtcgtcaaac gttgaaacaa tcatggtaga ggaatgtggt
     1081 ctggtaaaag accgtcctgt catcgtcggt gtttccggcg gaccagacag tctctgccta
     1141 atggaaacgc ttcgccgcgc agggtaccat ctcatcatcg ggtactttga ccacaaactc
     1201 cgccctgagt ctgcactaga tgcgcgcatg gtagaaaaag ctgccgtgcg gttgggcttg
     1261 ctatgtattc tggggggcat ggatgtgcgt tcctatgcag aagaaaagaa actctctatt
     1321 gaagaagccg cgcggacgtt aagatatcgc ttcctctttg aacttgctag aaaatataag
     1381 gcacaagccg ttgccgttgg gcacacagca gatgaccaag tagaaacggt ccttatgcat
     1441 tttttgcgtg gaagtggtat ttcgggcttg aagggtatgt cgtaccggtc ggttatcaaa
     1501 tcctttgacc cagatattcc agtggttcgc ccgttactgg atatgtggcg tgaagagacc
     1561 gttctgttct gtgcggttaa tggcttacgc ccaagttacg actcgagcaa tgagtctttg
     1621 aactttcaac gcaaccgcat tcggcatttg ctcattccct cacttgaaac atacaatcca
     1681 aaatttcgcg agacggtttt gcgcatgtca cattccctaa aaggggatta tgccttcgtt
     1741 atggaaacgc tggaacatgc ttggggcaat gtcgttgttt ctgcgaatga tgagattatc
     1801 accttcgatg ttttgcgcct atcttccatg ccgatcggac tccaacgtaa cctgattcgc
     1861 catgccatgc agactcttct cccaggcgtg gacattgggt accaaacctt gaaacgcgct
     1921 tctgatttta ttaatgcttc cagtgctacg gttccgcgtt tggacttaaa agcaggtcta
     1981 cgtatgttcc gcgaagccgg gctgattcat atctgcgcgc cttatgctga gcttcctttt
     2041 gaccgttggc cccaaatggt ggatgacaaa tcggttccat taagactggg cgggcaggct
     2101 tcactagctg gtgggtggaa ggtgatctgt gaaagttggc gtctgccaac attggcgaga
     2161 gaacaagccg aagaaaacga ggaccagttt caggtctggt tggatgcgga tgaacttcct
     2221 gaagaatttc tattgcgggc tcgtcgtcag ggcgatcatt tttctccgct ggggatgggt
     2281 ggtcattctc aaaaactttc agactttttt gtcaacgaga agctgccgca tcgtgcccgt
     2341 gaccgttggc ctctcttgtg tgtgggtgat gaaatcattt gggttccagg ctttcgcccg
     2401 gcacatcgct accgtttgaa agagacgact cataatgtga tttatttttc ggttcaacat
     2461 ccgcccgaga agattgcccc agatgaaaat ccccatccgt agtggatggg gattttttat
     2521 tttatttgac ttcgccgttt aattgcttga gtttgatgaa taactcgcgc cactggattt
     2581 ttgatacgcc tagtttttgg cggatcttat ctggttcaat gccgttctga taatctcgta
     2641 aggcagatgt ccagcgacac atatcgaagg agagatgttt ttccaaaccg gcttcttcgc
     2701 taatatcttc aagcagatat tccaaacggc gcggtgacca ggggaaagct tggtctacag
     2761 ggctgtattg tgtgaggtat tcgttgtagg cttcaaccca atcctcggtt agctcaattt
     2821 tgcgttcttt gtagcggttg gcagggctgg catagcgcac aaaaaggaag ggaccattct
     2881 taccagccaa ctcaatgtga ttgacatgaa tgccaaggca ttcacttttc ttgatggcag
     2941 tttgaagtaa gagagacaac aaggtgtatg ggcgggcatc tggcttcttg gcgcggcgct
     3001 gacggtctgc tgccaaaagc accgcttcat attcttcgtc ggtaagaacc tgtggcaacg
     3061 ggctaatggc agatcgttgc agaacttttt cggcagggtc aatgcttaac gtgccgtatt
     3121 gatgaagcca gcggaagaaa gacttaaccg atgtgatacg acgcgacaag gttttggggc
     3181 tgcatgccac gccacgacct ttttccatcc actcgaagaa gcggttgaga tcatcagtgg
     3241 tgacggtgcc aatggcttga tctggcggca ggaactgggt aagcaaccgt acatctgaga
     3301 gaaaggcttt gacggtattg ggggagcgtc cctgatcgag caggaacatt tcccaaccgt
     3361 tgatggcggc aggtaacagc gttttggcgc tgatgtgggc aggagcggag ttttttgtca
     3421 tacgaatcaa ccttgcgtgt agtatttact cttgcgtata gtttagcgaa tttatagggt
     3481 gttgtcaact ggcaaaaaaa gaaaccctcg aagtagaaaa cttcgagggt tgagcgcgtc
     3541 aaatactaag gcggtggtaa ataatcccac gggttgacct tgacacccgc catgatctca
     3601 aagtgtaagt gtgcacctga agaacgacct gttgacccaa ctgcaccgat tacttcacct
     3661 tgcccaacac tctgtccgca ccccacattt agcgcgctta agtgggcata caacgattga
     3721 aagccattgc catgatcgac cataatcata ttaccgtacc catagtcatt ccaaccagcg
     3781 tatacgatca cacctgcatc agaggcgtat acagcttcac cggtgttgcc ggcaaaatcc
     3841 aagccgttgt ggttagtctg tggtgaccag tcgaagccgg aaagatagtg cttgttggtg
     3901 gggtagatga aggagccata acccacagcg ccaccagaga ctgggtcaca ggcaccagcc
     3961 ccaagtacgc gtgccgaggc ggggttttcg cgcgtcacac ccagtggcgc actccaactt
     4021 acaaagtcac gtttcccatt tgggatgatg agccaagtcc cagctttgat gttcgcattt
     4081 tcataatcac caaccgtggt tgggtctata ttgttgccgg gatattcgat gatatctgcg
     4141 gcatttacgc cgtaaaactt tgcccattcg ccaaagggaa ttccgcccag ccattcccaa
     4201 tacacaccgt ccaccggaag aatggttaac tcttgacccg gtttgagtga gtggggatca
     4261 tctagcaacg tgttgtaatt gccccataaa attgtttgcg gtttcaaacc aaatttttct
     4321 gcaataccaa aaacagtatc accatcttgc acaacataag tggaaacttc attgcgcggg
     4381 cggcttggaa tggtagtatg caattgagct gcgcgattga tcccatcggt gacggggata
     4441 tcaagcactg caagagactc tgcgggcgcc gcctcaatat ctggacctga gtcctgattc
     4501 ggcgctacgg gcatatcttg cgcctgacgg taaaacgacc gagacataaa gaccaccaac
     4561 acaatggcaa caactgaaaa gagatttacc ccaagtttaa ttaatggctc gcctaacccc
     4621 atattcacaa ggctgtttat aagtcttgta aatagtgagg gaccatttga ggttccccca
     4681 gcgcgttgag aggttggttt tcctgagggg gtaggtgcat cttctttaaa ttcgttttgc
     4741 atagggttca tgataacatg gcaaaaattt cacagtcaag ttggggtgta aaaccctcat
     4801 acagccttaa taagaaattt gctctgtagg ctcatttttt cgaaaaaata gcaatcgcac
     4861 gactgaaatg ccaactaggc aggcaatacc acccgtcaca attgcaaatt gtacatctcg
     4921 cgtttgagcg atccaaccga taagtatgct cccgaacgga gcgacaccct gcaagcccca
     4981 aaagtaaatg ctgaagacac gtccacgcag atcgttggga acgcgcattt gaatcatggt
     5041 attcatgctg ataagctgga cgaccgtgcc ccaaccaatg aagatcaaca aaaagaatgc
     5101 cagagaggcg tttgtggtta atcctaatac gataatgggg aaaataaaaa ggatttgccc
     5161 ccacagtaat gtggagtttt tatttgcagt cgcaaaatac gccatcataa atgcggcagt
     5221 cacggctccg atgccttgag cggtgtagag attgctggtg cgggttgcga tggcggcttc
     5281 agaagtgaga agaggct
//
LOCUS       DAOQNJ010000119         5285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9336_length_5285_cov_7.518929, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000119 DAOQNJ010000000
VERSION     DAOQNJ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9336_length_5285_cov_7.518929"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..450)
                     /locus_tag="PLG52_04875"
     CDS             complement(139..450)
                     /locus_tag="PLG52_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB40810.1"
                     /translation="MSDIYFTDTKSKAVFSAEGPKPQFLIDTPKFKSLVVGLEAGGQI
                     PVHPGEAAMYHFLEGEGLMSVGDETFAIKPGVTVVVPSGVKRGMNAKTRVVFLGAKGE
                     A"
     gene            672..2846
                     /locus_tag="PLG52_04880"
     CDS             672..2846
                     /locus_tag="PLG52_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB40811.1"
                     /translation="MNSKQPRHTPTFRTSQFAVLNKLISMTHAWTDTQTVLSTALEQA
                     AQLAMADGAECHLVNSSGELQFTTQYNLDIVFMSGSLDIRFPIGKGLPGLAISNQSAF
                     LIPDIENEENYQRQHLAQQARYCSLICVPLSGMNSLLGIIMLYFRKRIQPDAVLLEIL
                     TAIGQHLGVSIERAHLFQQTSEQLKELQILQTVANALNRSANIQEALEQSLEAVVTTM
                     NMRCGWVVLLDGFQKNRLAASYNLPPELDPADWSAMRTHCRCIELLQLGKLDAAIKIV
                     ECQQLKKVKSPDYPYHSHASIPVRVGTLLLGNLNIVPPSGSAITIDNYRLFNSIGDQI
                     GVAIERARLYEQAKEQRTLEQQILLGHGQMLLGERKLQIIVNQTIKVVSNALRVEYVT
                     LVLVASDGKISLETDLGFSPRKTQDTVDVLLADNSAIVQSIRLKMPIINPDINLERQL
                     KANLVDENIIMTSSLIVPMLMGEEALGSIAVYSQSPRQWSEDEIRLLSLLANQTAIAI
                     ENTRLLEAEHTARKHAEILHLQTIQQAQDLILAYDTTIEGWSRALDLRDKETEEHTLR
                     VTNLTLQLAQAFDISHVELKHIKHGALLHDIGKMGIPDNILRKAGELSDDEWVMMRQH
                     PQLAYEMLSPIAYLLPALDIPYCHHEKWDGTGYPRGLKGEEIPLAARLFAVVDVFDAL
                     TSDRPYRPAWAKNKAIEYIRQKAGSHFDPSVVEVFLRLIDNK"
     gene            complement(2905..3588)
                     /locus_tag="PLG52_04885"
     CDS             complement(2905..3588)
                     /locus_tag="PLG52_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB40812.1"
                     /translation="MQNDILLRRLQDFEFLRGLDHETMTALANSAMWKVFPPHAVVFW
                     EGDTESNLYYLQYGSLKALRTAPDGREQVLRFLDAGEIFNEIGVLAKRPNPVTAVALE
                     ESGIWLIPRHALEEVVMKYPQTALQIIGNMADKIISLVTLAADLSLKTVEARFAKLLL
                     DSTEGDVIERRRWTNQTELAAHLGTVPDVLSRVIRELTKAGLIEMDKQHIRILNRASL
                     AERAMLQED"
     gene            complement(3723..>5285)
                     /locus_tag="PLG52_04890"
     CDS             complement(3723..>5285)
                     /locus_tag="PLG52_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNB40813.1"
                     /translation="VDTSNADVSLSEEDFVWAKQTFFDRCAGCHGTLRKGATGPALTP
                     DLTLAKGTVALSSIIFNGTLKGMPDWGKQGFFTQEQTDIMAKYLQNEPPTPPEMSMEQ
                     MKATWKVFIAPEDRPTEPQTTRNWQNYFSVTLRDAGQVAIIDGDTYEIVAKVDTGYAV
                     HISRMSATGRYVYVIGRDGKLALVDLWMEIPEKVAEVQTCYDARSVEVSKYEGELGDF
                     TDKYAIVGCYWPSHFTILDGQTLEPMKITSVRGYTADTNTYVGDPRVAAILASEFKPE
                     WIVNVKETGQVWLVNYQDPMNLTIKMINSALYLHDGGWDSTQRYFLVAANQSNKIVVV
                     DALEGDLEAIVDTPEVPHPGRGANWIDPEFGPVWSTPHLSANSLIAIGTDPEGNPDSA
                     WKVVRNIELPGAGSLFVKTHPNSKWVWVDFVLNSDEKLQRTVCVIAKENPTEVYKCWE
                     AADYGRAVHFEYNMDGTEVWVSIWGSADQPGKTGEIVIYNDETLEEIARIKDLITPTG
                     KFNVYNTIHEVY"
BASE COUNT         1383 a         1298 c         1195 g         1409 t
ORIGIN      
        1 ccatcacaaa gttataaaca ggcagccaac ccagcacaga gtagccggga ttccaaccgc
       61 gcattgccat gccgcccgca aagatcgaga gagcgccaat cacaaaagca agcacgcctg
      121 cggtcttcat tttggagatc acgcttcacc cttcgcgcct aaaaacacta cgcgcgtttt
      181 tgcgttcatg ccacgtttca cgccgcttgg caccaccacc gtcacgccgg gtttgatggc
      241 aaaagtttca tcgcccaccg acatcaagcc ttcaccttcg agaaagtgat acatcgccgc
      301 ctcgccaggg tgaacgggaa tttgcccgcc tgcctccagc ccaacaacca gtgatttgaa
      361 ctttggcgtg tcgatcagga attgcggttt tggaccttct gccgaaaaga cagctttcga
      421 ttttgtatct gtgaaataga tgtcggacat atcgtctcct ttgatatgcc tgactttact
      481 ggaagtcccc gcctactgcc ccgacttttg tcgggttttt caacccaaac ctcaatctcc
      541 ccgaatcatg gctcgctccg ccaaattgat atcattacac gagcgaacta ccgaataatc
      601 tctattacaa tcaacctatc ctcggaagta atcaactact gataatgctt ttcactgcat
      661 atggataaac catgaattct aaacaaccaa ggcatactcc gaccttccgc acatctcaat
      721 ttgctgtact aaacaagcta atctcaatga ctcatgcatg gaccgatacc caaacagtct
      781 tatcaacggc attggagcaa gctgcacaac tagcaatggc agatggggca gaatgtcatt
      841 tggttaattc atctggggaa ttgcagttta cgactcagta caacttagac atcgttttta
      901 tgtccggctc tttggatatt cggtttccca ttgggaaagg cttacctggt cttgcaattt
      961 caaaccaaag cgctttttta atccctgata tcgaaaacga agaaaactat caacggcagc
     1021 atctggcaca gcaggcgcgt tattgttcgc tgatatgtgt cccgctttcc ggcatgaact
     1081 cacttctggg cataattatg ctgtatttta gaaaaagaat tcaaccagat gctgttttgc
     1141 tagaaattct gactgccatt ggacaacacc ttggtgtttc aattgaacgc gcgcatctct
     1201 ttcagcaaac ctcagaacaa ttaaaggagt tacaaatatt gcagactgtg gcaaatgcct
     1261 tgaatcgttc tgcaaatatt caagaagctt tggaacaaag cttggaggct gttgtaacaa
     1321 ccatgaatat gaggtgcggc tgggttgtac tattggatgg tttccaaaag aaccgacttg
     1381 cggcgtccta caacctgcca cctgaattag accctgccga ctggtctgcc atgcgaaccc
     1441 actgtcgttg tattgagttg ctgcaactcg gaaagctgga tgcagcaatc aaaatcgttg
     1501 aatgtcaaca actaaaaaaa gttaagagtc ccgattaccc ttatcattcc catgccagta
     1561 tacctgttcg tgttggaact ttgctgttgg gcaatctcaa tatagtgcct ccatcaggct
     1621 ctgccattac gattgataat tatcgactct tcaactccat tggcgatcag attggagttg
     1681 caattgagcg cgctcgcttg tatgaacagg caaaggaaca gcgcactctc gaacagcaaa
     1741 tcctgctcgg gcatggacaa atgcttttgg gagaacgcaa actgcaaatc atcgtgaacc
     1801 aaaccataaa agtcgtttct aatgcgttac gggttgaata tgttacattg gttttggttg
     1861 cctcggatgg aaaaatttcc ctggaaactg atttgggatt ctcgccccgg aaaacacagg
     1921 atactgttga tgttttgctt gccgataact ccgccatagt tcaatctatt cgattgaaga
     1981 tgcccatcat taatccagat attaatcttg aaagacaatt gaaagcaaac ttggtagatg
     2041 aaaatataat aatgacatca tcattgattg ttccaatgtt aatgggcgaa gaggcactag
     2101 gcagcatagc agtatattcc caatctcccc ggcaatggag cgaagatgag attcgtttgc
     2161 tttccctgct tgccaatcag acagcaattg ccattgagaa tacccgcctg cttgaagctg
     2221 agcacactgc ccgtaaacat gcagaaattc tccacttgca aacaatacaa caagctcaag
     2281 atctgatcct ggcatacgat acaactatcg aaggctggtc tcgcgcactc gatttgcgcg
     2341 acaaagaaac agaagagcat accttgcgcg ttaccaacct aactcttcaa ttggctcaag
     2401 ctttcgatat tagccatgtc gaattaaaac atataaagca tggtgcatta ctccatgata
     2461 ttggaaaaat gggtataccc gataatattc ttcgaaaagc aggagaacta agcgatgatg
     2521 agtgggtaat gatgcggcag catcctcaac ttgcttatga gatgctgtct cccattgcat
     2581 atttactacc cgcgctggac attccgtatt gtcatcatga aaaatgggat ggcactggct
     2641 acccacgtgg gctaaagggg gaggaaattc cgctggctgc acgactattt gccgtggtag
     2701 atgtctttga tgcgctgacc agtgaccgcc catatcgccc tgcatgggca aaaaataaag
     2761 ctattgaata tatccgccaa aaggcaggtt cacattttga tccaagcgtt gtagaagtgt
     2821 ttttaagatt gattgataat aaataatatg caatatactt tgtctgaggt gctatctcat
     2881 agtgtcgggt tttcaaccca aacattaatc ttcctgaagc atggctcgct ccgccaagct
     2941 tgcacggttc agaatacgga tgtgttgttt atccatctcg atcagccccg ccttcgtcaa
     3001 ttcacgaatg acacgactga gtacatcggg cacagttccc aagtgtgccg ccaactccgt
     3061 ctgattcgtc cagcggcggc gttcaattac atccccctcg gttgaatcca ataatagttt
     3121 ggcaaagcgt gcttcaaccg tctttaacga cagatccgcc gcaagcgtga ccaggctgat
     3181 aatcttatct gccatgttgc caataatctg caacgccgtc tgcggatatt tcatcaccac
     3241 ttcttctaac gcatggcgtg gaataagcca aatgcccgac tcttccaaag ccaccgccgt
     3301 caccgggttg ggacgtttcg ccagcacgcc gatctcattg aagatctcgc ctgcatccag
     3361 aaatcgtaat acctgttcgc gtccatccgg cgcggttcgc aaagctttca acgaaccata
     3421 ttgcaggtaa tacaaattag actcagtatc accctcccaa aatacgaccg catgaggcgg
     3481 aaatactttc cacatggcac tattggcaag agctgtcatg gtttcatgat ccagtccgcg
     3541 caggaattca aaatcttgca gtcgtctgag aagtatatcg ttttgcatta gggtccttgg
     3601 ctatgaatcg tcttattcat attttgataa tactttatca cacatgttta taatgatctt
     3661 tccttaataa aaaggactgt cgacttcatg tcgacagtcc tttttattaa tttacttagc
     3721 ttttaataga cctcatgaat ggtgttgtag acattgaatt tgccagtagg ggtgataagg
     3781 tctttgatgc gggcaatttc ttcgagggtt tcatcgttat agatgacgat ttcgcctgtc
     3841 ttgcctggct gatcggcaga gccccaaata ctgacccaga cttcggtgcc atccatgttg
     3901 tattcaaagt ggacagcacg tccgtaatct gcagcctccc aacatttgta aacttctgtg
     3961 gggttttcct tggcaatgac acatacggtg cgttgaagtt tttcatctga gttcaataca
     4021 aagtctaccc aaacccattt actattggga tgtgtcttga cgaatagact gcccgcgccg
     4081 ggaagttcaa tattgcggac aaccttccag gcgctgtcgg ggtttccttc aggatcggtg
     4141 ccaatggcga tgagagaatt ggcgctgagg tgtggcgtgc tccaaacggg accaaattca
     4201 gggtcgatcc agttggcacc gcgtccggga tggggaactt ccggtgtgtc tactattgct
     4261 tcgagatcac cttcaagcgc atccaccacc acaatcttgt tggactgatt ggctgctacc
     4321 aggaagtaac gttgcgttga gtcccagccg ccatcatgca agtacaaggc agagttgatc
     4381 atcttgatag tcaggttcat cgggtcttga taattaacaa gccagacctg cccggtctct
     4441 ttaacattga cgatccattc gggcttgaac tcagaggcga ggatggcagc cacgcgcgga
     4501 tcacccacat aagtgttggt gtcagcggtg tagccgcgca cgctggtaat tttcattggt
     4561 tcgagggttt gcccatcgag aatggtgaaa tgggaaggcc agtaacagcc gacgatggca
     4621 tatttatcgg tgaaatcccc caattcgcct tcatatttac tgacttcaac cgaacgagca
     4681 tcgtaacaag tttgtacttc agcaactttt tcagggattt ccatccaaag atcaacaagt
     4741 gcaagcttgc catcacgtcc aatcacgtag acatagcgtc cggtagcgga catgcgcgag
     4801 atgtgaactg cataaccagt atccactttg gcgacgattt cataggtatc gccgtcaatg
     4861 atcgctacct gaccagcatc gcgcagtgtg actgagaagt aattctgcca attacgtgtg
     4921 gtttgcggtt cggtggggcg gtcttcggga gcaatgaaaa ccttccatgt tgctttcatt
     4981 tgttccatcg acatttcagg cggggtgggc ggttcgttct gcaggtactt tgccatgatg
     5041 tcggtttgtt cctgcgtgaa gaagccctgt ttgccccagt cgggcatacc tttgagggtg
     5101 ccattgaaaa tgatcgagga gagggcaacg gttccttttg caagagtcag gtcgggggtg
     5161 agggcaggac ccgtcgcgcc ttttcgcagc gtcccatgac agcccgcgca acgatcaaag
     5221 aaggtttgtt ttgcccatac aaaatcttct tcggacaggg agacatcggc gttggatgta
     5281 tccac
//
LOCUS       DAOQNJ010000120         5225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9520_length_5225_cov_7.443520, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000120 DAOQNJ010000000
VERSION     DAOQNJ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9520_length_5225_cov_7.443520"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..3949)
                     /locus_tag="PLG52_04895"
     CDS             complement(137..3949)
                     /locus_tag="PLG52_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB40814.1"
                     /translation="MASEHTIISQRNSTGMLIVKEGADAGMRFALRQGTTIIGREKGV
                     DIVLVDKQCSRQHCQIISLQGQFMIEDLKSTNGTRVNGASVTTMLKLNPGDQIAVGET
                     LFSFEIQKAEVITPYEKILKEQGVVPSRYVLSNAIATAQNLGHENLGFLSEVSGFIPT
                     TPPLLNLPPAYHAWDELVDNLPDLYRTLGLRAALEQMPILSADADTLPDKYLLRASMI
                     ISILGHAYFRIQTDPPEKLPESVMQPWKKITQRLGRPGPVLSYIDLIIYNWKLVDPQL
                     KDPITVENMRLMVPTVDNIVERIFYLGQVEILSRLTPVIGAAVRAQEAVYQDDPDALK
                     HELMLITDALHQTTYHSLQKIDLNPHNKSYHLDTVIWAKTVGPLAVSLEESTPGPSGI
                     ASPIFHLLDEFLGRQTYATRLGEEMTHIRDWYPPNWQNFLKAIGQISVPEYIEKSKSK
                     KLKGIFQETRQAYVAETGFLGRHRLKAYGFLETAFKVGRSVTIGSFSGKFKDRAWDEV
                     HSQLNNSQLERSSGHPQYYHFAEIKSVNTLTSDGQNWVKQLVLNTSGTGIRYQVGDRA
                     AILPENSDALVDKTLQALRARGREAIPLNKAWREGIVQREGYGDTPTLPLRTLLTFGR
                     IRPVDRHIAKLLYAITHNSTLKQIIEARAEDQWELWDLLDLISKAGYDTRKFWKASPG
                     ELESICRIVPPEVSRMYSVSSAMSNNERSSGASELHFTIGNLRYQTNETEVSRHADRQ
                     GASSNFLTDTTGIAAEDMGKVSFRVVHPPRFSMPSEETIPIVMIAGGTGFAPFRSFIQ
                     ERARQSQPGETWLFFGTRTKTDIYYQDELARIISQGRLNLRIAFSRDDVRFKQVDGNQ
                     LVFEPGERHSIGDEILKDENAQALWNLLQSREDGGQGAYFYVCGRTGFASAVMEAIKA
                     VIYRYSPGSEKEKQEAVKKTLYDLVGKGRYLQDIFSTYTGSHLDAQKTSYNASEIALH
                     NDDEHGYWMIIDGRVYDVTEFAQLHPGGVKIIREYTGMDATQPYRKILHNVNPEVDSM
                     LGMYEIGKVRRLHFGIQWGVYVSSDGLKAVMLSDVYRIWMRYLYFVVELENSLHNEFT
                     IQDQSTTANEKPAERSPYKVQLIIQVYKRFMNEYINSLTGEPLDMLWAVTSGLCSTSE
                     DVNQIRRSVESVQQSENARFVKGVINGLHEALLKQVREKPGQTDLPKDAVTAYFDLLE
                     AENKAFMKELRMSVLAGIRVFEEFEQDTVNKGGGRLIDAAKDIPSKLESYYAKVASAI
                     RSFGPNVK"
     gene            complement(4076..4537)
                     /locus_tag="PLG52_04900"
     CDS             complement(4076..4537)
                     /locus_tag="PLG52_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB40815.1"
                     /translation="MSELKLLQVKDVMETRTVRIQQDANMYRAAEFVAMSHASDLMVV
                     GEDDKFIGVLSEGDILRAALPDMEEISAEGGSVDTAFGIFLKKGKELSSLPILPFVIK
                     EPIVLDSQDHVAKAATLMIDLNIRLLPVIKDGKLVGTISRANICQAVVGNS"
     gene            4808..5086
                     /locus_tag="PLG52_04905"
     CDS             4808..5086
                     /locus_tag="PLG52_04905"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HNB40816.1"
                     /translation="MATFIMFGKYTMEATQGMDSKRTDQAMSLLKENGGEFKAGYALL
                     GGTDLVLIVDLPSTEAAMKFSAVLSKLQGISFTTSPAVSVADFDKLMK"
BASE COUNT         1277 a         1261 c         1282 g         1405 t
ORIGIN      
        1 tttgttcaga gaggatgtaa agttggatcc cggcgatgaa gagcaggggc agggccacaa
       61 aacgaacgaa gtaacgggga aagggggagt tgggattgaa catgatatga ccttctatcg
      121 ataatttata ttgcttttat tttacgtttg gtccaaaaga acgaattgca gaggcaactt
      181 tggcataata ggattcgagc tttgatggaa tatctttcgc agcgtctata aggcgcccgc
      241 ctcctttatt aaccgtgtcc tgctcaaact cctcaaacac acgaatgcca gccagcaccg
      301 acattcttaa ttccttcatg aaggctttat tttcggcttc caacagatca aaataggcgg
      361 tgactgcatc cttcggtaga tccgtttgcc ccggcttctc ccgaacttgt ttcagaagtg
      421 cttcgtgcaa gccattgatc actcctttaa cgaagcgggc attttctgat tgctgaaccg
      481 attccacgct cctgcgtatc tggttgacat cttcgctcgt tgaacacaat ccgcttgtta
      541 cagcccatag catatccaag ggttctccgg taagcgaatt gatgtattcg ttcataaagc
      601 gcttgtaaac ctgaatgatt aattggacct tgtatgggga gcgttcggca ggcttttcgt
      661 tggcagttgt cgattgatcc tggatcgtaa attcattgtg taaagaattt tccagctcga
      721 ccacaaaata caggtaacgc atccagatcc gatatacgtc cgaaagcatc actgccttca
      781 agccgtcaga gctgacgtag acaccccact gtatcccaaa atggaggcgg cggaccttcc
      841 cgatctcata catacccagc atggaatcca cttcagggtt tacattgtgc aggatcttcc
      901 gataaggctg ggtggcgtcc atgcctgtat attcgcggat gatcttgacg ccgcccgggt
      961 gcagttgggc aaattcggtg acatcataga cccgcccatc aatgatcatc cagtagccgt
     1021 gttcgtcatc attatgcagt gcaatttcag aggcattgta ggacgttttt tgtgcatcca
     1081 gatgagaccc ggtataggtg gaaaagatat cttgcaagta ccgtcccttg cccacaagat
     1141 cataaagagt ctttttgacg gcttcctgct tttccttttc agaacccggg ctgtagcgat
     1201 aaataaccgc cttgatcgct tccatgaccg cgctggcgaa tccggttcgc ccgcatacat
     1261 agaagtaagc gccctgtccg ccgtcttccc ggctttgtaa taagttccaa agcgcctgcg
     1321 cattctcatc ttttaggatt tcatccccga tcgagtgtct ttcgccaggt tcaaaaacca
     1381 gttgattccc atccacctgc ttgaagcgga catcatcccg agaaaaagcg atgcgcagat
     1441 tcaaacgccc ctgcgagatg atgcgggcaa gttcatcctg atagtaaata tctgttttgg
     1501 tacgtgttcc gaagaagagc caggtctcac ccggttgtga ctgtcgggca cgctcttgga
     1561 tgaaactccg aaacggcgca aacccggtcc cgccggcgat cattacaatg ggaatagttt
     1621 cttcactggg catgctaaag cgcggcggat gaaccaccct gaaggaaacc ttgcccatat
     1681 cctctgcggc gattccggtg gtatcggtaa gaaagttcga agatgcaccc tgtcgatctg
     1741 catggcgcga gacttcggtt tcattcgttt gatatcgcag gttcccaatg gtgaagtgca
     1801 gttcagaagc tccgcttgag cgctcgttgt tgctcatcgc cgatgagacg gagtacatgc
     1861 gcgaaacttc aggtggcacg atgcggcaga tactttccaa ctcacccggc gatgctttcc
     1921 agaattttcg ggtatcgtaa ccggctttcg agatcaggtc gaggagatcc caaagttccc
     1981 actggtcttc tgctctcgcc tcgatgatct gtttgagagt tgaattatgg gtgatcgcat
     2041 aaagtaattt ggcgatgtga cgatccacgg gtctgatccg cccgaacgtt agcaatgtgc
     2101 gcaaaggaag cgttggcgta tctccatatc cctctcgttg cacaataccc tcacgccagg
     2161 ctttgttgag cggaatcgcc tcacgtccgc gcgcccgcag cgcttgcagg gtcttatcga
     2221 ccagggcatc gctattctct ggcaagatcg cggctcgatc cccaacctga tagcggatac
     2281 ccgttcctga ggtattgagc accaactgtt tgacccagtt ctgtccatcg gaagtcagtg
     2341 tgttgacgga tttgatttct gcaaaatgat agtactgtgg atgtccgctg ctgcgctcaa
     2401 gctgtgagtt gttcagctgc gagtgaacct catcccatgc ccggtctttg aacttgccgg
     2461 aaaagctgcc gatcgtgacg ctgcgcccga ccttgaatgc ggtctccaga aacccatatg
     2521 cctttaagcg atgacgcccc agaaaaccgg tctctgcgac atatgcctgc cgggtctcct
     2581 ggaagattcc ctttaatttt ttgctcttgc ttttttcaat atactctggc acagaaattt
     2641 gcccgatggc tttcaggaaa ttctgccaat ttggcggata ccaatcccga atgtgcgtca
     2701 tttcctcacc cagacgggtc gcgtatgttt gacgtcccag aaattcatcc aatagatgga
     2761 agatgggcga agcaataccg cttggccccg gtgtgctttc ttccagagat actgccaatg
     2821 ggccaaccgt ctttgcccag ataacggtat ccaaatgata ggatttatta tgtggattca
     2881 gatcgatctt ctgaagggaa tgataagtag tctgatgtaa tgcatccgtg ataagcatca
     2941 gttcatgctt taatgcatcc gggtcatctt gatatacagc ttcttgtgcg cggacagctg
     3001 ctccgatgac gggcgtcagg cgggagagga tctcaacctg ccccaggtag aagatgcgtt
     3061 caacgatgtt atcgaccgtg ggcaccatca gacgcatatt ttcaacggtg atcgggtctt
     3121 tcagttgagg gtcgaccagt ttccagttgt agatgatcag gtcaatataa gagagaaccg
     3181 gcccgggtct gcccaaacgt tgcgtaattt tcttccaggg ttgcatgaca ctttcgggga
     3241 gcttttccgg cggatcggtt tgaatgcgaa agtaagcatg ccccaatata ctgatgatca
     3301 tggaagcccg caacagatat ttgtctggca gtgtatctgc atcggcgctg aggatgggca
     3361 tctgctcgag tgcggctctc agcccaagag tccggtaaag atcgggtaaa ttgtccacca
     3421 gctcatccca ggcatgatat gcgggaggca ggtttagcaa gggtggggtg gtggggataa
     3481 agccagagac ctctgagaga aaccccaggt tctcatgccc aagattctgg gcggttgcaa
     3541 tggcatttga aagaacgtag cgcgatggga cgaccccttg ctccttcagt attttttcat
     3601 agggcgtgat gacttctgcc ttttggatct caaagctgaa taaggtttcc ccgacggcaa
     3661 tttgatcgcc aggattcagc ttgagcattg ttgttacact tgccccattt acccgagtcc
     3721 cgtttgtgct cttaaggtct tctatcataa attgaccttg tagcgaaatg atctggcaat
     3781 gttgccggga gcattgctta tctaccagca cgatatcgac acctttttca cgacctatga
     3841 tcgtggtgcc ctgccttaag gcaaaacgca taccagcatc cgcaccttct ttaacaatca
     3901 gcattccagt ggaatttcgt tgggaaatga tcgtatgttc tgatgccatc gcatggctcc
     3961 ggggatatat cttattgata agaggattct ttaataagga gatcgtgctc cctgatgaaa
     4021 ggaatccgtg aggttacatg gaaatgaata gctggtttga agcaacagtt gactgttatg
     4081 aattgccaac aacagcctgg catatgttgg cacgtgaaat ggtgcccacc agcttgccgt
     4141 cttttatgac aggcaacaga cggatattga ggtcgatcat taaagttgct gctttcgcca
     4201 catgatcctg ggagtcgagc acaatgggtt ctttaataac aaagggaagg atgggaagac
     4261 tggagagttc tttgcctttt ttgagaaaaa tgccaaaagc tgtatccact gagcccccct
     4321 cggctgaaat ttcctccata tcggggagcg cagcccgtaa aatatcccct tcggatagca
     4381 caccaataaa tttgtcatct tctcccacga ccatcaggtc gcttgcatgg gacatggcta
     4441 caaattcagc ggcccgatac atattggcat cttgctgaat tcggaccgtg cgcgtttcca
     4501 tgacgtcttt tacctgtaaa agttttaatt ctgacatgtt atttcctaaa tcggaacgta
     4561 tctaaatgat caatcaacgc ttcagcttat tttattatat caaaggttta ttttttttcg
     4621 cctaataagg tgtataatgt ccacttatga actaatttat atacataagc gaatgaattg
     4681 aggaattggg cggaaatgtt tgtataacag caaagcatac acaagaatcg tttccgttct
     4741 ggagtttata cgagtgactt cgataaggca ctcacaacca ttagaggtaa agaaaggata
     4801 ggtttgtatg gctactttta tcatgttcgg taagtacaca atggaagcca cgcaaggcat
     4861 ggattcaaaa aggactgacc aggcaatgag tttgcttaaa gagaatggcg gtgagttcaa
     4921 agccggttat gctttgctgg gcggtaccga tcttgtgctg attgttgacc tgcccagcac
     4981 agaggccgca atgaagttct cggctgttct cagcaaacta caaggcattt cattcaccac
     5041 ctcacccgcg gtgagcgtgg ctgactttga taagttgatg aagtaatatc acccaagtcg
     5101 taaacttaca atgaggtatt gaggatgaag cagccgctcc actggagcgg ctgccttgtt
     5161 gtgcctaaag gataagtaca gttggctatt tgcgacccag ttacttaatc caccgtcaac
     5221 gtctt
//
LOCUS       DAOQNJ010000121         5215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9549_length_5215_cov_10.329845, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000121 DAOQNJ010000000
VERSION     DAOQNJ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9549_length_5215_cov_10.329845"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..260
                     /locus_tag="PLG52_04910"
     CDS             51..260
                     /locus_tag="PLG52_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40817.1"
                     /translation="MEAVECRSDTEYAERPLSLVWQGQRYEIAEIFSRWHGPGEKGFC
                     VKTTNGLAFELTYQEISDNWHVQPL"
     gene            270..1448
                     /locus_tag="PLG52_04915"
     CDS             270..1448
                     /locus_tag="PLG52_04915"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB40818.1"
                     /translation="MQEVNEVQRLSKRVAGLKPSGIRKFFDIAATMKDVISLGIGEPD
                     FTTPKPILDAGIRSLQNGETHYTSNSGKIELRQGVADNLKKLYNVKYDPANEIIATVG
                     VSEALYLTFTAVLEPGDEVIIPTPCFVSYQAEVILAGGVPVEIPARIENSFTVDPDDI
                     RKAITPRTKIIFIGYPSNPTGAVAPREVMVEIGKIAEEHNLLVVSDEIYDRLVYDFEH
                     VCFPALSDNLWHRTVLLGGFSKDYAMTGWRIGYACAPADLIKGLVRIHQYTIMSAPTT
                     AQDAAIEALKTGEPYVAEMVAEYDRRRRLLVDGLNRLGLTTFEPKGAFYTFPNIRASG
                     MDDETFAEKLLREESVAVVPGNAFGPGGEGFVRMCYATSYEQIEEALRRMERFMSRHG
                     "
     gene            complement(1496..1834)
                     /locus_tag="PLG52_04920"
     CDS             complement(1496..1834)
                     /locus_tag="PLG52_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40819.1"
                     /translation="MPENSRTVWSSDEGDQRKKTQTTTTHSRSLPPQQQTAYLHRESG
                     GRGGKVVSVVKNLVLTEEDMKTLAKKLKQECGTGGTVKDGIIEIQGEHRQRMAEVLTK
                     LGYKVKIAGG"
     gene            complement(1838..2713)
                     /locus_tag="PLG52_04925"
     CDS             complement(1838..2713)
                     /locus_tag="PLG52_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40820.1"
                     /translation="MIKLTDVTKRYDNTTVVHPLNLQINEGEIVGIIGHNGAGKSTTM
                     KMIAGLVEPTSGEVRVMGQDMWKHNLKVKQRIGYLPEESPLYETMTARQYLLFFSELY
                     QMPRAKALSRIDGLLESLDLPQKDKLTGEFSKGMKRKTAIARTLLHNPDLLILDEPNS
                     GLDPLTSFFIINYLKTLKREGKTILLSAHNLFHVETICDRVAIIKNGRLLVFDTMDSI
                     RSRLGKREYQVIFHSDDKLEYEQLGGNYIFRTHDVDGIARMLETVSAKGWTLVDLSMR
                     ESALEEIYVKLMTNE"
     gene            2797..4425
                     /locus_tag="PLG52_04930"
     CDS             2797..4425
                     /locus_tag="PLG52_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB40821.1"
                     /translation="MNQILVIAWREITRLRKRFGGGGSPLTVLILLGVLGFTSYTLRD
                     SVTLGNGMYRVGVSGNVPAINDSRFAVMEVGADEGQALLAAKKIDLLIVDTQVFSRED
                     DKSQFAERAMKQYMEKEELVRVGNSFAEGEAFPLRVGINYLGAGQPSAGNEAGTSAPQ
                     TTKPEEVIIPSLTPPPAPFTQVLVALIYILPITFISIFFTSSFMDEKANRRLTVLLSA
                     PVSPFQIIFGKMLPYAIFALSTTVLIAYFTKANILLALAIFAPTTLFIFAIYLMVPLF
                     YRTFKDTTFISMLVTTMTTAYLVFPAMFTNSSDLAYISPLALAVKMYRNEPFGWREYL
                     LPSLPMTAVFVFAMFAGTRLLNEEFLMGYKPLTRKVSDAIYLMMAHSKPWFAVMLWSF
                     LVIPLVYMAQVVMLAFATNLPVRLIVGAVLLAAGFIEEVVKTVGIVVLAERGVIKSVR
                     EILWLSFLSALGFLIGEKLLVFTSVSVVAQAEVSTALFQAGVFLLIPLAAHFIFVSVV
                     NLLRFKLRFPYWLALAVGTILHVIYDATVLRGGI"
     gene            4422..>5215
                     /locus_tag="PLG52_04935"
     CDS             4422..>5215
                     /locus_tag="PLG52_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB40822.1"
                     /translation="MIYPFLAIVKKELSSVLRDRTIVISILIQFFIASFSSGLLLGML
                     SLYDADTIMKFSGPNMRIGVIESADTALADRIEERGLNVIRFETLAEAQTAFYNNEIL
                     AIVDTPTDVNGLTEVKLYLPDSDTVSSLIRMVIQEPLKQYENDLRQANGVEIYYTDLK
                     GKPATSFEFVYSVLIPMLMFFPAFVAGSMSIDAITEEVEGKTLQTLLSTPLTVSTMIG
                     AKIVSAVILAVLQCAAWLALIQINGTVIQNRAWILLLALVIAGITST"
BASE COUNT         1236 a         1195 c         1313 g         1471 t
ORIGIN      
        1 tcatgaagtt aaatgtaggg gatacgagtt caatcgaact gtgagtccgc atggaagctg
       61 tagagtgcag gtcagataca gaatatgcgg agcgtccgct ttcgttggtg tggcaggggc
      121 agcgctatga aattgcagaa attttctcgc gctggcacgg acccggtgaa aaaggatttt
      181 gcgttaagac cacgaatggt ctggcgtttg aattgacata ccaggaaatt tcagacaact
      241 ggcatgtaca acctttataa ggaggctcaa tgcaggaagt aaatgaagtt caaagacttt
      301 caaaacgtgt ggcgggtttg aagcccagcg ggattcgaaa atttttcgat attgccgcaa
      361 cgatgaagga tgtgatctcg ctcggtatcg gtgagccaga tttcaccacc cccaagccga
      421 ttctagatgc cggtattcgt tcactacaaa atggtgaaac gcattacacc tcgaactctg
      481 gcaagatcga attgcgacaa ggtgttgcag ataacttaaa gaaactttac aacgtgaagt
      541 atgaccccgc taatgaaatc attgcgacag ttggggtttc tgaagcgttg tatcttacct
      601 tcaccgctgt gctcgaaccg ggtgatgaag ttatcatccc gaccccatgt tttgtttctt
      661 accaagcgga agtgatcctt gctggcggcg tgcctgtgga aattccagct cgcattgaga
      721 atagctttac tgttgacccc gatgatattc gcaaagcgat cactccacgc acgaagatta
      781 tctttattgg ttatccttcc aaccccaccg gtgcagtcgc tccgcgtgaa gtgatggtgg
      841 agattggcaa gattgcggaa gaacataact tgttggtggt atcagatgaa atttacgacc
      901 gcctcgtgta tgactttgaa catgtctgct tcccggcgtt gagtgataat ctttggcatc
      961 gcactgtatt gttgggcggt ttttcgaaag attacgccat gaccggttgg cgcattgggt
     1021 atgcttgtgc tccagcagac ctgatcaaag gactcgttcg tattcatcaa tacaccatca
     1081 tgtctgcgcc aacgactgcg caggatgccg cgattgaagc gttgaaaacg ggcgaaccct
     1141 atgtggcaga aatggttgca gaatatgacc gccgccgccg tttattagtg gatggattga
     1201 accgcctcgg gctgacgacc tttgagccga agggcgcctt ttataccttc cccaacatcc
     1261 gcgcctctgg catggacgat gaaacgtttg ccgaaaaatt attgcgtgaa gaaagtgtgg
     1321 ctgtggttcc gggcaatgcc ttcggacccg gcggcgaagg gtttgtgcgc atgtgttatg
     1381 ccacttcgta tgaacagatc gaagaagctc tgcgccgcat ggaacgcttc atgagtcggc
     1441 acggataagt ttataaataa aaaaagagga caggcattga gcctgtcctc tttttttatc
     1501 cgccagcaat ttttacttta taaccaagct tggtcaaaac ctctgccatg cgttgacgat
     1561 gttcgccttg aatttcgatg atgccatctt tcacggttcc acctgtgcca cattcctgtt
     1621 tgagcttctt ggcaagggtt ttcatatcct cttcagtgag cacaaggttt ttcaccacag
     1681 aaaccacctt ccctccccta ccacccgact cgcgatgtag ataggcggtc tgttgttgcg
     1741 ggggcaaaga tctagaatga gttgtggtcg tttgagtctt cttacgctgg tcgccttcat
     1801 cagatgacca aacagtgcgg ctattttccg gcatggtcta ctcgttcgtc attagtttga
     1861 catagatttc ttccagtgcc gattcgcgca tggatagatc caccaaggtc caacctttgg
     1921 cagagaccgt ttccaacatg cgggcaattc catctacatc gtgagtacgg aaaatataat
     1981 tcccgccaag ttgttcatac tctaacttat catctgagtg gaagatgact tgatattcgc
     2041 gtttccccaa ccgtgaccga atggagtcca tcgtatcgaa gactaacagt cttccattct
     2101 tgatgatcgc aacccggtcg cagatggttt cgacatggaa gaggttatgg gcgcttaaca
     2161 agatggtctt accttcccgt ttgagcgttt tgagatagtt aataataaag aaggaagtca
     2221 gcgggtcgag accggaattg ggctcatcga gaataagcaa gtcggggttg tgcaacaggg
     2281 tgcgggcgat agcggtcttg cgcttcatac ccttggaaaa ttcgccggtc aatttatctt
     2341 tttgaggcaa atccaacgac tcgagcagac cgtcaatgcg agagagggct ttggcgcgcg
     2401 gcatttggta aagctctgag aagaagagca aatattggcg ggcggtcatc gtctcataca
     2461 gcggactttc ctctggtaaa tagccaatac gctgtttgac cttgaggtta tgcttccaca
     2521 tatcctgacc catcacacgc acttcgcctg aggtcggttc gaccaaacca gcgatcattt
     2581 tcatggtggt gcttttgcca gccccattgt gcccgatgat tccgacgatc tcaccctcat
     2641 tgatttgtaa gttcaagggg tggacgactg tagtattatc gtaccgctta gtgacgtctg
     2701 taagcttgat catgatgcca atttatacca aacaaactca aggtaagggg tttgcagaag
     2761 acagaaactt ttgcttcctt acaaaagtgg aaacagatga atcaaatcct tgttattgca
     2821 tggcgtgaaa ttacccgttt aagaaaacga tttgggggtg gcggcagccc gttgaccgtg
     2881 ctgattttgc tgggcgttct gggcttcact tcatacactt tgcgtgactc tgtcacatta
     2941 ggcaatggaa tgtatcgtgt gggcgtatct ggaaatgtgc ctgctattaa cgacagtcgc
     3001 ttcgccgtga tggaagtggg tgcggatgag ggtcaagcct tgctggcagc gaaaaagatt
     3061 gacctgctga tcgtggatac acaagttttt tcacgtgaag acgataagtc tcagtttgca
     3121 gagcgggcga tgaagcaata tatggagaag gaagaacttg tccgtgtagg gaattcgttc
     3181 gcagaaggag aggcgtttcc gctgcgagtg gggattaact acctcggggc cgggcagccc
     3241 tcggcgggga atgaagcggg aacatccgcg cctcagacta caaaaccaga ggaagtcatc
     3301 atcccttcat tgactccacc tccggctcca ttcacacaag tgttggtggc attgatctat
     3361 attttgccca tcacattcat cagcatcttt ttcacgagta gttttatgga tgagaaagca
     3421 aaccgccgtt tgaccgtttt actttctgcg ccggtttctc cgtttcagat tatctttggc
     3481 aagatgttgc catatgcgat ttttgcgctt tccacaacgg ttctgatcgc ttactttacg
     3541 aaggcgaata ttctgctggc gctggcgatc tttgcaccga caaccctttt tatttttgcc
     3601 atctatttga tggtgccatt gttttaccgc accttcaaag atacgacctt catttctatg
     3661 ttggtgacga ccatgacgac ggcatatctt gtttttccgg cgatgttcac caacagcagt
     3721 gatctggcat acatttcgcc gctggcattg gctgtaaaaa tgtatcgcaa tgaaccgttt
     3781 ggctggcgtg aatatttact gccttcttta ccgatgaccg ccgtgtttgt gtttgccatg
     3841 tttgcaggga cgcgcctgct gaacgaagaa tttttgatgg gctacaaacc gctcacccgc
     3901 aaggtcagtg atgcgattta tttgatgatg gcacattcca agccctggtt tgcggtgatg
     3961 ctgtggagtt ttctcgtcat ccctttggtg tacatggcgc aagtggtgat gttggcattc
     4021 gccaccaacc tgcccgtgag attgatcgtt ggtgctgtgt tacttgcggc tggtttcatt
     4081 gaagaagtcg ttaaaacggt tggaatcgtg gtattggcag aacgcggcgt gattaagtct
     4141 gtgcgcgaaa ttttatggct ctcgtttctc tctgcattgg gatttttgat cggcgaaaaa
     4201 ctgctggtct ttacatccgt gagtgtggtt gcacaagccg aagtttcaac agcgttgttc
     4261 caagccgggg ttttcttgct tattccactt gccgctcatt tcatttttgt ctcggtcgta
     4321 aatttattga ggttcaaatt gcgttttccg tattggttgg cgttggcagt aggcaccatt
     4381 ctgcatgtga tctatgatgc gaccgtgtta cgaggtggaa tatgatctat ccattcttgg
     4441 cgatcgtaaa aaaagaacta agttccgtcc tgcgcgaccg gaccattgtc atttcgatct
     4501 tgatccaatt ctttatcgct tcgttttcct ccggtttgtt attgggaatg ctttcccttt
     4561 acgatgcgga tacgatcatg aaattcagcg gacccaatat gcgaatcggc gttattgaaa
     4621 gcgcagatac cgcccttgca gaccgcatcg aagaacgcgg acttaacgtg attcgttttg
     4681 aaaccttggc tgaggcgcaa actgcttttt ataacaacga aatccttgcc atcgtggata
     4741 caccgaccga tgtaaatggt ctgactgagg taaaactgta tttaccagac tccgatacgg
     4801 tcagttcgtt gatccgtatg gtgattcaag aaccgcttaa gcaatacgaa aatgacttac
     4861 gtcaggcgaa cggtgttgaa atttactaca ctgacttaaa aggaaaacca gcgacatcgt
     4921 ttgagttcgt ctattccgtt ctgattccta tgctgatgtt cttccctgcg tttgtagcag
     4981 gtagtatgtc tattgatgcc atcacggaag aagtagaagg caaaacgctg caaaccttac
     5041 tttctacgcc gctcaccgtc agcacaatga tcggtgccaa gattgtctcg gcggtgattc
     5101 tggctgtctt gcagtgtgcc gcatggctgg ctctcattca aatcaacggg actgtcattc
     5161 aaaaccgcgc ctggattttg ctactggcgc tggtcattgc aggaatcaca tccac
//
LOCUS       DAOQNJ010000122         5212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9556_length_5212_cov_9.208842, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000122 DAOQNJ010000000
VERSION     DAOQNJ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9556_length_5212_cov_9.208842"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="PLG52_04940"
     CDS             <1..1571
                     /locus_tag="PLG52_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNB40823.1"
                     /translation="YFKELGLTYIHLMPLFKAPEGENDGGYAVSSYREVHPPLGTMKQ
                     LSSLARDFREAGISLVVDLVFNHTSDEHIWAERAKAGDEEYMDFYRIFPSRELPNRYE
                     QNLREIFPEEHSGAFSPFIINGKEHWVWTTFHSYQWDLNYDNPAVFNRMAEEMLFLAN
                     QGVEVLRLDAVAFIWKQLGTNCENLPQAHTLIKAFNAVARITAPALIFKSEAIVHPDD
                     VVKYISLEESQISYNPLLMALLWNSLATRKVNLLSQALAHRFKLPQGTAWVNYVRVHD
                     DIGWTFSDEDAAILGVNGHDHRQFLNEFYRGRFPGSFARGLPFQENPITGDCRISGTC
                     ASLAGLEKALHEEGEKEVELAIRRILLIHGVIITVGGIPLIYLGDEIGTLNDYTYRDD
                     PAHERDSRWVHRPRADWERYKKRNNLHSLEGHIFHGFRSMIEMRKANDVFAGGDLEII
                     PTDNSHVLGFMRTHAGKRAVVFANFSETTQTLPPRIIEQYSITSLKKLHGHNHVSAKK
                     SLELDPLDFVVFGS"
     gene            1814..>3558
                     /locus_tag="PLG52_04945"
     CDS             1814..>3558
                     /locus_tag="PLG52_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNB40824.1"
                     /translation="METLVTPGFDPAHDVLHYGRQPLDSIFAPKSVAVVGATETVGSV
                     GRTIVWNLMSSSFSGTIYPVNPKRPSVLGIKAYPDLKSIPEKLDLIVVVTPAPTVPDI
                     IKEAVDLGVPSAIIISAGFKETGPAGVELERQVMEHARRGNMRIIGPNCLGVMSPTTG
                     LNATFASTIARRGSVGFISQSGALCTAILDWSLTENVGFSSFVSIGSMLDVDWSDLIY
                     YLGDDPHTKSIVIYMETIGNARAFLSAAREVAMTKPIIVIKPGRTEGAAKAAASHTGS
                     LTGSDEVLEVAFRRSGVLRVNSIAELFYMAEVLGKQPRPKGPRLTIITNAGGPGVLAT
                     DALISNGGELTTISPETMEEFNKLLPAAWSHNNPVDILGDASPERYAKALEIAAKDEN
                     SDGILVILTPQAMTDPTETADVLKPYAQGLGKPVLATWMGGKEVAPGEAILNKVNIPT
                     FPYPDTAARVFDYMAHYSENLHSLYETPMSMDDGGDGIDRERAAKLIEDVRKSGRTIL
                     TEYESKQLLACYGIPTVDTRIAKSKEEAVKAADAIGYPVVLKLHSETITHKTDVGGVQ
                     LNLADAKDVKKAYES"
     assembly_gap    3559..3658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3659..4306
                     /locus_tag="PLG52_04950"
     CDS             <3659..4306
                     /locus_tag="PLG52_04950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40825.1"
                     /translation="ELDINPLMASPEKLIALDARVLVYGKDVTQAQLPKMAIRAYPMK
                     YVSSWTAKDKTNVTIRPILTEDEKLLVRFHADLSDRSVFLRYLQPMMFQERVVHERLS
                     RICHCDYDREIALVAEKKVDGESIIMGVVRLSKLHANSEGRLSILVADPYQGTGLGGE
                     MVRRMIDVAKQEHLEKISAILTDDNQVMGQLFKKLGFTIEATAENPKLMNALLML"
     gene            complement(4353..>5212)
                     /locus_tag="PLG52_04955"
     CDS             complement(4353..>5212)
                     /locus_tag="PLG52_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB40826.1"
                     /translation="NWVDFDVEDGTLKLDDLQKALERKPKLVAVGYASNSLGTVNPVQ
                     KIVKMAHDAGALVYVDAVQYAVHGAIDVQKLNADFLVSSSYKYFGPHAGILYGKRELL
                     EKLVAYKVRPATNELPGKFETGTQNHEGIAGILGAIEYFEWVGKEFGGEYADELKQQG
                     YSGRRLTLKQAMSAIHAYEFELSRVTLSTLEAIPGIHIFGNTDPRRLDERIATFSFRI
                     GDMNPQTIAEKLAAENIYVWNGNYYAINVSERLGVEEKGGMVRVGPVHYNTVEEINKL
                     GDVLKKIAG"
BASE COUNT         1267 a         1346 c         1253 g         1246 t
ORIGIN      
        1 cttacttcaa agaacttggt cttacctaca ttcatctcat gccgctcttc aaagcacccg
       61 aaggcgaaaa cgatggcggc tatgccgtca gcagctatcg tgaggtgcat ccgccgctcg
      121 gtacgatgaa gcaactttca tcgctggcgc gtgattttcg tgaagcgggc atcagccttg
      181 tcgttgacct tgtcttcaac catacctccg atgaacacat ctgggccgaa cgcgccaaag
      241 ccggcgatga agaatacatg gacttctacc gcatctttcc tagccgtgag ttgcccaacc
      301 gctacgagca gaatctcagg gagatcttcc ccgaagagca ttcgggcgcg ttttctccat
      361 tcatcatcaa cggcaaagaa cattgggtgt ggaccacttt ccactcctat caatgggact
      421 tgaactatga caacccggcg gtctttaacc gcatggcaga agagatgctc ttcctcgcca
      481 atcaaggcgt ggaagtgttg cgccttgatg cagtggcttt catttggaaa caactcggca
      541 ccaattgcga aaacctgccg caagcacata ccctgatcaa ggctttcaat gcagtggcac
      601 gaatcactgc gcccgccttg atcttcaaat ctgaagcgat tgtgcatcca gatgatgttg
      661 tgaaatacat ctcgctcgaa gaaagccaga tttcttacaa cccgcttctg atggcattat
      721 tgtggaactc gcttgccacg cgaaaagtaa acttgctttc gcaagccttg gcacatcgct
      781 tcaaacttcc gcaagggacg gcttgggtga attacgtccg cgtgcatgat gatatcggct
      841 ggaccttctc cgatgaagat gccgccatcc tcggcgtgaa cggacatgac catcgtcagt
      901 tcctcaacga gttctatcgc gggcgcttcc ccggtagttt tgcacgcggc ttgcccttcc
      961 aagaaaatcc catcacaggc gattgccgca ttagcggaac ttgcgcctcc ttggctggtt
     1021 tggaaaaagc tctccatgaa gaaggcgaaa aagaagtgga acttgccatc cgccgcatct
     1081 tgttgattca cggtgtcatc atcacggtgg gtggtattcc gctcatttac ctcggtgatg
     1141 agattggcac cttgaatgat tacacctatc gtgacgaccc cgctcacgaa agagacagtc
     1201 gttgggtgca ccgtccgcgg gcagattggg aacgatacaa gaagcgcaac aacctgcatt
     1261 cattggaagg acatatcttt catggcttcc gttccatgat cgagatgcgc aaagcgaacg
     1321 atgtttttgc cggtggcgac ttggaaatca tcccgaccga taattcacat gtgcttggct
     1381 ttatgcgcac tcatgctggt aaacgggcgg tggtctttgc caacttctct gaaaccaccc
     1441 agactctccc tccgcgcatc attgagcaat actccatcac cagcttgaaa aaactgcatg
     1501 ggcataacca tgtctctgca aagaagagtc tggaacttga cccgttggat tttgtggtgt
     1561 ttgggtcttg atggtttttt gatggaactt gcaagaaact gacggaatca ttcgaaagcg
     1621 gatgtctttt taggaagaca tccgcttttt tgttgggaaa aaagcaagaa taaggaagtt
     1681 aatctataag aaacgtgaaa ttgagcaccc gcttctagtg gtatatatgt ctttgtatca
     1741 tcctgtcttc aaaactgatt gttacgatga aaaaacatcg taacaacgta ccctttaaag
     1801 aaagaagtaa attatggaaa cccttgtcac tcccggattc gaccccgctc atgatgttct
     1861 acattatggg cgccaacccc tagactctat ctttgccccc aaaagcgttg ccgttgtcgg
     1921 tgctactgaa acggttggca gcgtggggcg gaccattgtt tggaacttaa tgagcagctc
     1981 ttttagcggc accatttacc ccgtcaaccc gaagcgtcca agcgtccttg gcatcaaagc
     2041 ctacccagat ttgaaatcca ttcctgaaaa gttagacctt atcgttgtcg tcacaccggc
     2101 gccgactgtg cccgatatca tcaaagaagc agtggatctt ggtgtgccaa gcgccatcat
     2161 catttcggct ggattcaaag agacgggtcc ggcaggggtg gaacttgagc gccaggtgat
     2221 ggaacatgcc cgccgtggaa acatgcgcat catcggaccg aactgcctcg gtgtaatgag
     2281 cccaactact ggcttgaacg cgaccttcgc cagcactatt gcacgacgtg gaagcgtggg
     2341 cttcatcagc cagagtggcg cgctttgtac agctatcttg gattggagcc tcacggaaaa
     2401 cgtgggcttt agttccttcg tctccatcgg ctccatgctc gatgtggact ggagcgacct
     2461 tatttactat ctcggcgatg acccacacac caagagcatc gtcatttata tggaaacgat
     2521 tggtaatgca cgtgctttcc tttctgcggc gcgtgaagtt gccatgacca aacccatcat
     2581 cgtcatcaaa ccggggcgca ccgaaggtgc agccaaagca gcggcttctc acaccggttc
     2641 attgaccggt agtgacgaag tgcttgaagt tgctttccgt cgcagtggtg tgttgcgcgt
     2701 aaatagcatt gccgaattgt tctacatggc agaagtgctc ggcaaacaac cgcgcccgaa
     2761 aggtccacgc cttacgatca ttaccaatgc tggcggaccc ggcgtgctcg ccaccgatgc
     2821 actcatttcg aatggcggtg aacttaccac tatctcacca gagacgatgg aagaattcaa
     2881 caaactgctt cccgccgcgt ggagccataa caacccggtc gatatccttg gcgatgccag
     2941 ccctgaacgt tacgccaaag cacttgagat cgcagccaaa gacgaaaaca gcgacggcat
     3001 tttagtcatc ctcaccccgc aagccatgac cgaccctacc gaaacggcag acgtcttgaa
     3061 gccatacgca caaggcttgg gaaaacccgt cctcgctact tggatgggcg gcaaagaagt
     3121 ggcaccgggt gaagccattc tcaacaaagt gaacatcccc accttccctt atcccgatac
     3181 ggcggcgcgc gtcttcgatt acatggcgca ttattctgag aacctgcaca gcttgtatga
     3241 aacacctatg tctatggatg atggtggcga tgggatcgac cgtgaacgtg ctgcaaaact
     3301 cattgaggat gttcgcaaat ctggtcgcac cattttgaca gaatacgaat caaaacaact
     3361 tttggcttgt tacggcatcc cgacggtgga tactcgcatc gcgaagagca aagaagaagc
     3421 cgtcaaagcc gcagatgcca ttggctaccc ggtggtgctc aaacttcatt cagagaccat
     3481 cacccacaag accgatgtgg gcggcgttca actaaacctt gcagatgcca aggatgtgaa
     3541 gaaagcctac gaaagcatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     3661 attggatatc aacccgctca tggcatcacc tgaaaagctc atcgctttag atgcgcgtgt
     3721 gcttgtgtat ggcaaagatg tgacgcaggc gcaattgccc aagatggcga tccgtgcgta
     3781 cccgatgaaa tacgttagct cgtggacggc gaaagacaag accaatgtca ccatccgccc
     3841 gattttgacg gaagatgaaa aacttctcgt gcgcttccat gctgaccttt cggatcgcag
     3901 cgtattcctg cgttatctcc agccgatgat gttccaggaa cgtgttgtgc atgaacgcct
     3961 ctctcgcatt tgtcactgcg actatgaccg tgagattgct ttggtggctg aaaagaaagt
     4021 ggatggcgaa agtatcatca tgggtgtggt gcgtttgagc aaactacatg ccaatagcga
     4081 aggtcgtttg agcattctcg ttgccgaccc ctatcaagga accggcttgg gcggcgaaat
     4141 ggtgcgccgt atgatcgatg ttgccaaaca ggaacacctt gaaaagatca gcgccattct
     4201 aacggacgat aatcaggtga tgggtcagct cttcaagaag ttgggcttca ccattgaagc
     4261 gactgctgaa aaccccaagt tgatgaacgc tctgctgatg ctgtaatatc tttaaataaa
     4321 aactcccagt cattcgactg ggagtttttt gtttagcctg caattttctt taacacatcg
     4381 cctagtttgt tgatttcttc cacagtattg tagtggacgg gtcccacacg caccatgccg
     4441 cctttttctt ccacccccaa ccgttcagag acgttaatcg cgtagtagtt gccattccac
     4501 acataaatat tttcggcggc gagtttctca gcaatggtct gcggattcat gtcgccgatg
     4561 cggaaggaga aggtcgcgat acgttcgtcc aaccggcgcg gatcggtatt gccgaaaata
     4621 tgaattcccg gaatcgcttc gagtgtggaa agcgtcactc ggctgagttc aaattcataa
     4681 gcatggatgg cactcatggc ttgcttgaga gttaggcgac gtccgctata gccttgttgc
     4741 ttcaactcgt cagcgtattc accaccgaat tctttgccaa cccattcaaa gtattcaatg
     4801 gcaccgagaa tgcctgcaat gccttcgtgg ttttgggtgc cggtctcaaa tttgccgggc
     4861 aattcattcg tggctgggcg cactttgtag gcaacgagtt tttcaagcaa ttcgcgcttg
     4921 ccgtacaaaa tccctgcatg tggaccgaag tacttataag aagaagacac cagaaaatca
     4981 gcgttgagct tttgcacgtc aatcgcgccg tggacggcgt attgcaccgc atctacatac
     5041 actaatgcgc ccgcatcgtg tgccatttta acgatcttct gaaccgggtt caccgttccc
     5101 agcgagttag aggcataccc aacagcaacc agcttcggct tacgttcgag cgctttttgc
     5161 aagtcatcca atttgagcgt gccatcttcc acatcaaagt ctacccagtt ga
//
LOCUS       DAOQNJ010000123         5192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9598_length_5192_cov_8.999805, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000123 DAOQNJ010000000
VERSION     DAOQNJ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9598_length_5192_cov_8.999805"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1052)
                     /locus_tag="PLG52_04960"
     CDS             complement(66..1052)
                     /locus_tag="PLG52_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40827.1"
                     /translation="MVEIWAFIFRLKWSIEKDLFQTMKITLLTLAGLFILSACAQAVQ
                     SVPSPVIAESPTSTKSVETPTPNPSQTPHPTPTEWIKVYPTKKAILIYGESSRNEYTL
                     HFIEWGDFYLTPRMVLYEDGQLILGMGAYEKQLSQAETQTIVSKLEQLGFPKLQEAYD
                     VKSDSIFTVTPSMDLNPSWSKTEITLDVNGPKSITYHNYWGDLLTPPMKDIISYLANI
                     SSTAATPYQPDRLLVSAGDVTEIPEGKTVIPWPEGVTSPLHRSYMGVFYLEGDEASKL
                     YNATGEYLSEYFSYEGKIYEVNFRPILPHECHTYHLYEETPPAQPSFTCDDW"
     gene            complement(1006..1641)
                     /locus_tag="PLG52_04965"
     CDS             complement(1006..1641)
                     /locus_tag="PLG52_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNB40828.1"
                     /translation="MNELKLILVDPNPSLCDAFRERFKASQNVEIVTGVFERLSAFDC
                     MVSAANSFGLMDGGVDAAITRFFGDELQARVQKRIIDEFLGEQPIGTSIIVETMHPKH
                     PFVAHTPTMRVPMAIAHTDHVYSAMWAMLLAVRQHNLKSEKKINIIACPGLGTATGQV
                     PYRQAARQMALAYEHFLNPPQNIDWRFADKRQKSVRYGGDMGFHFPPEMVD"
     gene            complement(1718..3040)
                     /locus_tag="PLG52_04970"
     CDS             complement(1718..3040)
                     /locus_tag="PLG52_04970"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNB40829.1"
                     /translation="MCDTLIATKLATTNGIAVFGKNSDRPPNEGQHLAFFPAAEHALG
                     STVKCTYIEIPQAAHTHAVFLSKPYWMWGAEMGANEHGLVIGNEAIYSKVPANKEPAL
                     LGMDMLRAALERAVTPREAMQVIIELLEQFGQGGNCASHGNEMYYHNSFLIANAEDAW
                     VLETIDKQWAAKQVTDVYSISNCLTIGKQYDLSSADLLDFAMKKGMTKSQGEFHLAQN
                     FNDFLYTNFGKGLNRRETTFNALDSQKGKVNVETMMSTLRHHKDEHFDPRNGINDQDV
                     CMHAGFGPIRISQSTASLVAYMDKNNPILFATGTSAPCTSLFKPFWLDTASFLNAEPA
                     PTHEADANSLFWSHERLHRAVLENYRERLAAYANERDEMEARFITGALKLQNAPLQER
                     ADFTRACYAEALAAEADWLKRVQAVPSSKSIFHAMAWDGFSKKAGLAG"
     assembly_gap    3124..3223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3224..3480)
                     /locus_tag="PLG52_04975"
     CDS             complement(<3224..3480)
                     /locus_tag="PLG52_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40830.1"
                     /translation="MVHALSEIHRTLKPNGILLDLRPLEDSWSVEVVSTSSTQGVSGA
                     GWQVSGRLSDMPIGIADDAAANQAMREVESRGWFIQKQKEE"
     gene            complement(3499..3807)
                     /locus_tag="PLG52_04980"
     CDS             complement(3499..3807)
                     /locus_tag="PLG52_04980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB40831.1"
                     /translation="MSLQKDPENIEGKTLRRLLDFQDKCVLEVGCGEGRLTFKYAAAA
                     KFVAAFDVDMDALRVARADAKIKAGSQSEDYGTFACASARHIPFSKEKFDIAILAWSL
                     "
     gene            complement(3807..4232)
                     /locus_tag="PLG52_04985"
     CDS             complement(3807..4232)
                     /locus_tag="PLG52_04985"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB40832.1"
                     /translation="MAKNAELKTKKNDASVEDFVHAIPDEQMRKDCFEITKMMKRATK
                     AEPKMWGSSIVGFGEYHYKGASGREGDWMLVGFSPRKQNLTLYIIAGFEHYPEQMKKL
                     GKYTTGKSCLYIKKLADVDKKILQEIIVDSVKIMKKLNA"
     gene            complement(4287..4715)
                     /locus_tag="PLG52_04990"
     CDS             complement(4287..4715)
                     /locus_tag="PLG52_04990"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB40833.1"
                     /translation="MAKSAELKTKKNETSVAGFINAIKDEALREDCLEIAKIMKQVTK
                     SDGKMWGTSIVGFGEQHLVYASGRELDWMVMGFSPRKQNITLYLPGALETYQKLLDTL
                     GKYTTGKGCLYVKKLADVDKKVLKEIIAASAKEAKKVKTV"
BASE COUNT         1245 a         1239 c         1207 g         1401 t
ORIGIN      
        1 ggacggacag tccagggaaa aaggctaagg cgtagaaaac tgcattatga gagctccaca
       61 atcaactacc aatcgtcaca agtaaaggaa ggttgtgccg gaggagtttc ctcataaaga
      121 tgatatgtat ggcactcatg cggaaggata ggacgaaaat taacctcata aatctttcct
      181 tcataactga aatattcaga aagatactct ccggttgcat tgtaaagctt tgatgcttca
      241 tctccttcca aatagaatac gcccatatag gaacgatgta atggtgaagt tactccctct
      301 ggccatggaa tgactgtctt cccctctgga atttccgtta catctccagc agaaaccaac
      361 agacgatcag gttggtaggg agtcgccgct gttgaagaga tgttagccaa ataagaaata
      421 atgtctttca tcggcggggt tagcagatcc ccccaatagt tatggtatgt aattgatttt
      481 ggaccattca catcaagggt aatctcagtc ttagaccaag atgggttcag atccatacta
      541 ggagtaaccg tgaaaattga gtctgatttt acatcataag cttcttgtaa tttagggaat
      601 ccaagctgtt ctaacttcga gacgatagtc tgagtctcgg cttgcgagag ttgcttttcg
      661 taagcaccca ttccgagaat gagctggcca tcttcgtata aaaccatacg aggcgtcaaa
      721 tagaaatctc cccactcgat gaaatgtaga gtgtattcat tacgagatga ctcgccgtaa
      781 atcaatatgg cttttttggt tggatagact tttatccatt cagtgggtgt gggatgtgga
      841 gtttgacttg gatttggtgt aggagtctca acactctttg tcgacgtagg actttcagcg
      901 ataactggag aaggtacaga ttgaacagcc tgcgcacaag ccgaaagtat gaacagtcca
      961 gcgagagtga gcaatgtaat tttcatagtc tgaaacaagt ctttttcaat cgaccatttc
     1021 aggcggaaaa tgaaagccca tatctccacc ataacgaaca cttttttgcc gcttgtccgc
     1081 aaaacgccaa tcaatatttt ggggtggatt taggaaatgt tcataagcca aagccatttg
     1141 tcttgcggct tgccgatagg gaacttgacc tgttgctgtg ccaagacccg gacaagctat
     1201 gatgttaatt tttttttctg atttcaaatt gtgttgtcta actgccaaaa gcattgccca
     1261 catggctgag taaacatggt cggtgtgagc aatagccata ggaactcgca tcgttggcgt
     1321 gtgagcaaca aaagggtgtt ttggatgcat tgtttccaca ataattgacg taccaattgg
     1381 ttgctcgcca agaaattcgt caataatacg tttttgaact ctggcttgta actcatcgcc
     1441 aaaaaaacga gtaattgcag cgtctactcc gccgtccatt aatccgaaag aattagctgc
     1501 gctaaccatg caatcgaatg cagataatcg ctcgaataca cctgtgacaa tctcaacatt
     1561 ttgagatgcc ttaaatcttt cacgaaaagc atcacacagg gatgggtttg gatcaactaa
     1621 aatgagtttt aattcattca ttatgttcct ttgtttttgc caaagcaacc caaactatgt
     1681 tttatacaac atcctgctgt ataagtacct atatagttta cccggccagc ccggctttct
     1741 tgctgaaccc gtcccaggcc atggcatgga agatggattt actcgaaggt acagcctgga
     1801 cgcgtttcag ccaatcggct tcggcggcga gggcttcggc gtagcaggcg cgggtgaagt
     1861 cggcccgttc ttgaagcggg gcgttttgca gtttcaatgc gccagtgatg aaacgcgctt
     1921 ccatttcgtc gcgctcgttg gcgtatgcgg ccaggcgttc acggtagttt tccaacaccg
     1981 cgcggtgcag gcgttcgtgt gaccagaata gggagttggc atcggcctca tgggtggggg
     2041 caggctcggc gttaaggaag gaggcggtat ccagccagaa gggcttgaaa aggctcgtgc
     2101 agggggctga ggttccagtg gcgaaaagga tcgggttgtt tttatccata tacgccacca
     2161 gcgaggccgt gctttggctg atgcgaatgg gaccgaaccc ggcatgcata cacacatcct
     2221 gatcgttgat gccgttgcgc gggtcgaaat gttcgtcttt atgatggcgc agggtcgaca
     2281 tcatggtttc aacgtttact ttgccttttt gcgagtcgag cgcgttaaag gtggtttcgc
     2341 ggcggttgag acccttgcca aagttggtgt agagaaaatc gttgaaattt tgcgcgagat
     2401 gaaactcgcc ttgcgatttg gtcatgccct ttttcatggc aaagtccaac aggtctgcgg
     2461 aggaaagatc gtattgcttt ccgatggtga ggcaattgga aatggaatac acatccgtga
     2521 cttgtttggc tgcccattgc ttgtcgatgg tttcaagcac ccaggcatcc tcggcgttgg
     2581 cgatcaggaa actattgtga tagtacattt cattgccgtg cgaagcacag ttgccgccct
     2641 gaccaaactg ctcgagcaac tcgatgatga cttgcatcgc ttcacgcggc gtaacggcgc
     2701 gttcgagtgc ggcgcgcagc atatccatgc ccaacaaggc tggctcttta ttggcaggca
     2761 ctttggaata gatcgcctca ttgccgatca ccaacccgtg ttcgtttgcg cccatctccg
     2821 cgccccacat ccaatagggt tttgaaagaa agactgcatg tgtatgcgcg gcctgtggaa
     2881 tttcaatata ggtgcatttc acagtgctgc ctaaagcatg ctcggcggcg gggaaaaatg
     2941 caaggtgttg tccctcgttg ggcggtcggt ccgagttctt gccaaagacg gcaatcccgt
     3001 ttgtggtggc gagtttcgtg gcgatcaaag tatcacacat gcttggctcc ttatgtcatt
     3061 gcgaggagcg gtagcgacga agcaatctca aaattaacat gagattgctt cgtcgggcaa
     3121 agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaattcct ctttttgttt
     3241 ttgaatgaac cacccgcgcg attcgacttc cctcatagcc tgattcgccg ccgcatcatc
     3301 ggcaatccct atcggcatat cgctcaatct acccgatacc tgccagcccg ccccggaaac
     3361 tccttgggtt gagcttgtcg aaaccacctc caccgaccac gaatcctcca acggacgcag
     3421 gtcgagcagg atcccgtttg gcttgagcgt tctatgaatt tcactcagag catgaaccat
     3481 gctcgcatgt tccagtcatc aaagcgacca ggccaggatg gcaatatcga atttttcttt
     3541 tgaaaaggga atatgccttg cactcgcgca agcaaacgta ccgtaatctt cagattgcga
     3601 tcctgccttt atttttgcat ccgcccttgc cacccgcagc gcatccatat ccacatcaaa
     3661 tgccgccaca aacttcgcag ccgcagcata cttgaacgtc aatcgcccct caccgcagcc
     3721 gacctccaaa acacacttgt cttgaaaatc cagcaaacgg cgcagggttt tgccttcaat
     3781 attctccggg tctttttgca gagacattat gcattcaact ttttcattat ctttactgaa
     3841 tcgacgatga tctcttgcag gatcttttta tccacatctg ccagtttttt gatatacagg
     3901 caggatttgc cagtggtgta ttttcccagt tttttcatct gttcggggta atgctcaaaa
     3961 cccgctatga tgtacagagt gaggttttgc ttgcggggtg agaaacccac cagcatccaa
     4021 tcaccttcgc gtccgctggc gcccttgtaa tgatattccc caaagccgac aatgcttgag
     4081 ccccacatct ttggttctgc tttggtggcg cgtttcatca tcttcgtgat ctcgaaacaa
     4141 tctttacgca tttgctcgtc tggaatggca tggacgaagt cttcaacgct tgcatcgttc
     4201 tttttggttt tcaactctgc attctttgcc atgtttaact cctaaagttc ttgcagacat
     4261 gatggataag gccgaggaaa cgctatctac acggttttga ctttctttgc ttctttggca
     4321 gatgcggcaa taatctcttt caaaaccttt ttgtctacat ctgcaagttt tttgacgtac
     4381 aaacaacctt tgccggttgt gtactttcca agggtatcaa gaagtttctg gtaggtttcc
     4441 aacgcgccgg gcagatatag cgttatattc tgtttgcgcg gtgaaaaccc catgaccatc
     4501 caatccaatt cgcgtccact ggcgtagacc aggtgttgtt caccaaaccc gacaatgctt
     4561 gtgccccaca ttttgccgtc gcttttggtg acctgcttca ttatcttcgc gatctccaaa
     4621 caatcctcac gcaacgcctc gtccttgatg gcattgatga aacctgcaac acttgtttca
     4681 ttcttcttgg ttttcagttc tgcactcttt gccatgttca actccttttt aaagattgaa
     4741 gcgattttag catatttgtt ctgttttctg ggcgggtaaa aatgccggga gtgcctaata
     4801 cccaagacat aacccgtcat tgcgagcccg atagggcgaa gcaatcccca aatttgcgag
     4861 cgagattgtt tcttcgggcg acgccctcct cgcaatgacg gatgttttga tttcgatgtt
     4921 agatactctc aaaatgctgc ccttctacat gacctggctg taaaaggcag acgatgaaaa
     4981 tgctcccgtt accaagaatt gtaacgggag cgcggataca aacgggctga tcgttatttc
     5041 aaatacttat ccatccaccg caaaatgtga ttcaaccgcg caatgcgccg atctgtgcga
     5101 ccaacgcgtg aaagtccatg cggttcttcg gggaagcgca cgaactccgt ctccacgcct
     5161 gcgcgtttga gcgccacgaa ggcttgctcg cc
//
LOCUS       DAOQNJ010000124         5181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9630_length_5181_cov_7.085447, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000124 DAOQNJ010000000
VERSION     DAOQNJ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9630_length_5181_cov_7.085447"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PLG52_04995"
     CDS             complement(<1..1211)
                     /locus_tag="PLG52_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB40834.1"
                     /translation="MHEITLIINIVVALVVAFIGGVIARRLGLPTIVGYLFAGIAIGP
                     FTPGFVGDTHTISQLAELGVIFLMFGVGLHFSLSDLWKVRSIAIPGALGQMALTTLLA
                     FFLSRSWGWDASSGIVLGLAISIASTVVLLRGLMDKGLLNTAHGQAAVGWLVLEDLAT
                     VLILVLMPTLANTSGPFDWAGLGLTLVKASAFVILLLFVGKRFIPWVLLQIAHTRSRE
                     LFILAILAITLGTALGAAELFGVSFALGAFIAGVVVSESPLSHQVGADVFPFREAFAV
                     LFFVSIGMLVNPIYLLNNIGYVLALTALIVIGKSLITLLLGSVFPWQGRTALVVAAGL
                     SQIGEFSFILGQSGIDLGILTQDQYSLILAGALLSITVNPLMFRLIDPAENWFKKRPA
                     LWRLLDRHRPLT"
     gene            complement(1332..2648)
                     /locus_tag="PLG52_05000"
     CDS             complement(1332..2648)
                     /locus_tag="PLG52_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB40835.1"
                     /translation="MINRFRNWASTPIIEDENKSLSANLLNLIIWILVISTSLYGIIA
                     PIEPEFLNQRLFFIIPYILINLVAKQLLNNGYIDLVGHFIVICLWGVITSALFVGADN
                     KNPAFMGYIVVVTCAGLVLNRKAAILWALISIITSALILQFGILGFLPRSANETPAFA
                     FWSAQALYIAVCSMLLTQTLQKIEEARNRAQKELLERKRVEAERERIIGELEMKNAEL
                     ERFTYTVSHDLKSPLITIGGFIGLLEKDTLNGDVNRLKNDLEKIREAKDKMARLLDEL
                     LELSRIGRVKNPSGEFSLKQIVDEAVTLVQGRITSGRVQVIIHEPLPMLYCDGPRLVE
                     VMQNLLDNAAKFMGDQPTPRIDISARNDADEIIVYIKDNGMGINPIFHTKIFNLFDKL
                     DPKSEGTGAGLALVKRIIEVHEGRIWVESDGAGTGSCFCFTLPAKT"
     gene            complement(2735..3295)
                     /locus_tag="PLG52_05005"
     CDS             complement(2735..3295)
                     /locus_tag="PLG52_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNB40836.1"
                     /translation="MQKEIIIAGFGGQGVLFGGQVLAYAAMDNGLDVTWIPSYGPEMR
                     GGTANCTVVIADHEIGSPLVKNPPLAIALNLPSFDKYEAALAKGGTLVVNKSMVDHNQ
                     RRDDIHALFVPCNEIAEEIGDRKLVNMVAVGALLTALPEVKLEDVEKALEGHLPARHK
                     HLLPKNFEALKRGFEHAQKEWDKVPA"
     gene            complement(3297..4067)
                     /locus_tag="PLG52_05010"
     CDS             complement(3297..4067)
                     /locus_tag="PLG52_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB40837.1"
                     /translation="MATPNNLVYERPEAFTEMPTHYCPGCTHGVAHRLVAEVLDEMNL
                     VEKTIGVAPVGCSVFAYNYFDTDFVEAPHGRAPAMATGVKRVLPDRVVFTYQGDGDLA
                     SIGMGEIVHAAARGENITVIFINNANYGMTGGQMAPTTLPGMKTSSSQSGRDVETQGY
                     PIKVSEMISTLDGVGYCVRRSLHDPKNIRLAKKAIRTAFETQVRGLGFSMVELLSTCP
                     TNWGMTPVNSLKFVEEHMIPAYPLGDYKISDAIKQIKV"
     gene            complement(4067..>5181)
                     /gene="vorB"
                     /locus_tag="PLG52_05015"
     CDS             complement(4067..>5181)
                     /gene="vorB"
                     /locus_tag="PLG52_05015"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNB40838.1"
                     /translation="KRMPKELWKGNEAIAESAVRAGLGAYFGYPITPQTEVLEYLSRR
                     MPELGRAFVQAESELGAINMVYGAACTGVRVMSSSSSPGMSLMMEGLSYIAGTEIPAV
                     LVNVMRGGPGLGNIAPAQSDYNQAVRGGGHGDYPRIVLAPASVQEAIDLTALSFDLAE
                     KYRMITLIILDGTTGQMMEPAEMPAMQEMHRKNFEWATNGAMNRERRVLSSIYLNPLD
                     EEQANLRMLRNWKNVQANEVRYKEYFLDDAKYVVVGFGTAGRVALSAVREARAQGIKV
                     GLLRPITVSPFPYEALEQLIPQAEAFLVAEMNMGQMLDDVLMTVRGRVPVEFYGRPGG
                     VVPFHNEILDEIHRLASGPLAVTTDPRDSWLTRMTA"
BASE COUNT         1363 a         1334 c         1231 g         1253 t
ORIGIN      
        1 gtgagcgggc gatgacgatc taacagccgc cacaaagccg gtcgtttttt gaaccaattc
       61 tcggcggggt caatgaggcg gaacatcagc gggttgaccg tgatcgagag cagcgctcca
      121 gccaggatca aggaatactg atcttgagtc agaatcccca aatcaatgcc agactgacca
      181 aggatgaacg agaattcacc gatctgactc aagcccgccg caacgaccag agccgtgcgc
      241 ccttgccacg ggaagaccga gccaagcaat agcgtgatga gtgacttgcc aatcacgatc
      301 aaggcagtta gagccagaac atacccaatg ttgttgagaa gatagatcgg gttgacgagc
      361 attccgatgg aaacgaaaaa caaaacagcg aaggcttcac ggaaggggaa gacatctgct
      421 ccgacttgat gactcagcgg cgactcactg acgaccacgc ctgcgatgaa tgcacccagt
      481 gcaaaggaca cgccgaataa ttctgctgcg cccagagccg tgccaagtgt gattgccaga
      541 atagcaagga tgaacagctc acgtgaacga gtatgcgcga tctgcaaaag cacccagggg
      601 ataaaacgtt tgcccacgaa taaaagcaga atcacgaagg cacttgcttt gacgagcgta
      661 agccccaaac ctgcccaatc aaatggtccg ctggtatttg ctagagtggg catgagcacc
      721 agaatgagaa ccgtggcaag gtcttccaac accagccagc cgacggcagc ttgcccatgt
      781 gctgtgttaa gcaaaccttt atccatcaag ccgcgcaaca acaccaccgt gctcgcaatg
      841 gaaattgcca aacccagcac gatgcctgat gatgcatccc agccccaaga gcggctgaga
      901 aaaaatgcca acaatgtggt gagtgccatt tgccccaatg cgccagggat ggcgatggaa
      961 cggactttcc aaagatcgct cagtgaaaag tgcagcccga cgccaaacat caggaagatg
     1021 acccctaatt cggcgagctg actgatcgta tgtgtatctc cgacaaaacc cggggtgaaa
     1081 ggtccgatgg cgatgcctgc aaataaatat cccacaatgg taggcagccc aagccgccgc
     1141 gcgatgactc cgccaataaa agccaccacc aatgccacaa caatattgat gatcaatgta
     1201 atttcgtgca tatttcctcc ataaactatt aagttagctc tcggttcgac aaaatcattg
     1261 tacacaaaaa atccgcattc cagtctggaa tgcggatttt taataaacct attaccttat
     1321 atcatttgag gttacgtttt agcgggtaat gtaaagcaga aacaactacc tgtaccggca
     1381 ccatctgact cgacccagat ccgaccttca tggacttcaa tgatgcgctt gaccagcgcc
     1441 agcccggcac cagtcccttc actttttggg tccagcttgt cgaataaatt aaagatcttg
     1501 gtatgaaaaa tgggattaat acccatgcca ttgtctttaa tgtatacgat aatttcatcg
     1561 gcatcattcc ttgcgctgat gtcaattcgc ggtgtgggct ggtctcccat aaattttgcg
     1621 gcattgtcca atagattttg cattacctct accagccgcg gaccatcaca atataacatt
     1681 ggtaaaggtt catgaattat cacttgcact ctcccagatg tgattctgcc ttgaaccaat
     1741 gtaaccgcct catcgacgat ctgtttcaat gaaaattcac cagatgggtt tttcactcgc
     1801 ccaatacgtg agagctccaa aagctcgtct aacagacgcg ccattttatc ttttgcttcg
     1861 cggatctttt ctaaatcatt ctttagccga tttacatctc cattaagtgt gtctttttcg
     1921 agtagcccga taaagccgcc gatagtgatt agcgggcttt ttaggtcatg agagacggtg
     1981 tatgtaaaac gctctaactc cgcatttttc atctcaagct caccaatgat tctttcgcgt
     2041 tctgcttcaa ctcgtttgcg ctcaagcaat tctttttgcg ctcgattgcg agcttcttca
     2101 attttctgaa gagtttgcgt taataacatg gagcagactg caatatacaa ggcttgagca
     2161 gaccaaaaag caaaggcagg tgtttcattt gccgagcgtg gtaaaaaccc aagaataccg
     2221 aattgtaaga tcaaggcact ggtaataatg gatatcaacg cccataagat ggcggctttt
     2281 cggtttaaga ccaacccggc acaagtcacc accacaatat agcccataaa ggctgggttt
     2341 ttattatctg cccctacgaa taatgcactg gtaatgactc cccataaaca gatcacaata
     2401 aaatgcccaa ccaagtcgat atatccgttg ttgaggagtt gttttgcaac tagatttata
     2461 aggatataag ggatgataaa gaaaagacgc tgatttagaa attccggctc aatcggggcg
     2521 ataatcccat ataaactcgt tgaaataacc aatatccaaa tgatcaggtt gagcagattc
     2581 gcagaaaggc ttttattttc atcttcgatg ataggtgtac ttgcccaatt gcgaaatcga
     2641 tttatcataa gagtctccag cagaataaac ctgattaaac cataaataaa agcccacctc
     2701 agaaattaga gatggacttt tattatcagt ttatttaagc cgggacttta tcccactctt
     2761 tttgcgcatg ttcaaagccg cgcttcagag cctcgaagtt ctttggcaag agatgtttgt
     2821 ggcgagcagg taaatgacct tcaagcgcct tctctacatc ttcaagtttt acttcaggca
     2881 gagcagtaag caaggctcca accgccacca tgttgaccag tttacggtca ccgatctctt
     2941 cggcgatctc gttgcatgga acgaacaatg catggatgtc gtctcggcgt tgattatgat
     3001 ccaccatgga tttattgacc accaatgtgc cgccttttgc cagtgccgct tcgtatttgt
     3061 caaatgaggg gaggttgagg gcaatcgcca gcggtggatt tttgaccagc ggggagccga
     3121 tttcatgatc tgcaatgacg actgtgcaat ttgccgtgcc gccgcgcatt tctggaccat
     3181 acgaggggat ccacgttaca tctaagccat tgtccatggc tgcgtaggca agtacctgcc
     3241 cgccaaacaa aacgccttgt ccaccaaaac ctgcgatgat gatttctttt tgcatgttac
     3301 acctttatct gcttgatcgc atcactgatc ttgtaatcac ccagcgggta ggcagggatc
     3361 atatgctctt ccacgaactt caatgagttg accggggtca tgccccagtt tgttgggcaa
     3421 gtggagagca attcgaccat cgagaagccc aatccgcgca cttgagtctc aaacgcggtg
     3481 cggatggctt tcttcgcgag acgaatattc ttcgggtcat gcagggagcg gcgcacacaa
     3541 tagccgaccc catccagcgt ggaaatcatc tcagatactt tgatgggata gccttgtgtt
     3601 tccacatcac gcccgctttg agaagaggag gtcttcatgc cgggtaaagt ggtcggagcc
     3661 atttgtccgc cggtcatgcc atagttggcg ttgttaataa agataacggt gatgttctct
     3721 ccgcgtgctg ccgcatgcac aatttcgccc atgccgatgg atgccaagtc accatcgcct
     3781 tgataagtga ataccacacg gtctggcagt acacgcttca caccggttgc catggcgggc
     3841 gcacgtccgt gcggggcttc aacgaaatcg gtgtcaaagt agttgtacgc aaagacagaa
     3901 cagcctaccg gtgcaacacc gattgtcttc tcaaccagat tcatttcatc taacacttcg
     3961 gctaccagtc tatgtgccac gccgtgcgta cagcccgggc aatagtgggt gggcatttcg
     4021 gtgaaagctt caggtctttc gtaaacaaga ttatttggag tagccattat gcagtcatcc
     4081 ttgtcagcca ggaatctctt gggtctgtgg taacagccaa aggtccagaa gcgaggcgat
     4141 gaatttcatc caaaatttca ttgtggaagg gaaccacgcc gccgggtcgc ccgtaaaact
     4201 ctaccgggac acgcccgcga acggtcatca aaacatcatc gagcatttgc cccatattca
     4261 tttcagcgac gaggaacgcc tcagcctgcg ggatgagctg ttccagcgct tcataaggaa
     4321 acgggctgac ggtgatcggt cttaaaagcc cgaccttgat gccttgcgcg cgcgcctcgc
     4381 ggaccgccga aagcgcaacc cgcccagccg taccaaaacc aacgacaaca tatttggcat
     4441 cgtcgagaaa atattctttg taacgaactt cattggcttg cacgttcttc cagtttctca
     4501 acatgcgcag gttggcttgt tcttcatcca atgggttgag gtagatcgaa gaaagcacac
     4561 ggcgttcgcg gttcatcgcg ccgttggttg cccactcaaa attcttgcga tgcatttctt
     4621 gcatggcggg catttcagcc ggttccatca tctgaccagt tgtgccatcg agaatgatga
     4681 gcgtgatcat gcgatatttt tcagcaaggt cgaaggagag cgcggtcaag tcaatggctt
     4741 cttgcacgga tgcgggagcc agcacaatgc gtggataatc tccatgcccg ccgccacgca
     4801 cagcttggtt gtaatcactt tgagccgggg caatattgcc caagccgggt ccgccgcgca
     4861 tcacgttgac caacactgcc gggatttctg ttccggcgat ataagacaac ccttccatca
     4921 tcaggctcat gcccggtgaa gaggaggaag acatcacgcg cacgcctgta catgccgcgc
     4981 catagaccat gttgattgcg ccaagttcag attcggcttg aacgaaagca cgtcccagtt
     5041 cgggcatgcg gcgcgagagg tattcgagga cttctgtctg tggggtgatc gggtatccaa
     5101 agtatgcccc caacccagcc cgcacagcag attctgcgat ggcttcattg cctttccata
     5161 attcttttgg cattcgtttc t
//
LOCUS       DAOQNJ010000125         5132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9780_length_5132_cov_10.047272, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000125 DAOQNJ010000000
VERSION     DAOQNJ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9780_length_5132_cov_10.047272"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLG52_05020"
     CDS             <1..754
                     /locus_tag="PLG52_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNB40839.1"
                     /translation="VMFGEESFHDDKDLDTALFLQKLKVAKSLPKTAAPEPSLYYPIL
                     EEARKNDQSVVVVAPTGKASGTVRSAQTAAQEFPEVDVRIVDTQTISCNLGSLVLLAD
                     DMAKVGKSASQIVAKLEDMIPRGRIYFLVDTLEYLAKGGRIGGAKRLVAELLEIKPIL
                     QIKDGQVESFEQQRTKKRALARLVEITKEQCSGGAESHLCVLQVEAEKEAQALVEELK
                     STIPVAEIPIYELPPAIVVHAGPRAMGIGFFV"
     gene            809..1615
                     /locus_tag="PLG52_05025"
     CDS             809..1615
                     /locus_tag="PLG52_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNB40840.1"
                     /translation="MLELSNTRFAFLDVETTGLSPWFGDRICQIAVIVTEGKRIKRTL
                     DLLVDPERELSPSAIHVNGLDAVALKGKPRFAEIANEVEEALKDAVVVCHNAKFDIQF
                     LDNEFRKLGRTIEFPNLIDTLLLARDYYELPSYSLDQLSQDFHISANMQGSHALADAI
                     VMKNLFFTMADSLKTIDKTLDDLIGIYNSPAWPEEGIYLPTELSEAIHSGKKLFIQYM
                     DKDGEISERWVSPREVIGLSDYLYLQAYCHSREADRTFRLDRILSVEAEK"
     gene            complement(1646..2194)
                     /locus_tag="PLG52_05030"
                     /pseudo
     CDS             complement(1646..2194)
                     /locus_tag="PLG52_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
     gene            complement(2313..3908)
                     /locus_tag="PLG52_05035"
     CDS             complement(2313..3908)
                     /locus_tag="PLG52_05035"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNB40841.1"
                     /translation="MKNNPNKLWIFVLALGWLFDFLFWKQAPGVNFAIFWTACLIVGF
                     YLLLSNGHRPHRTTLLLIPLFGFFAAVTFIRAEPMTTFLAYTFTMFTLTIFTVTYLGG
                     RWVQYALADYVGKLLSLVGSMLIRPITFTADVRKTQAEAGTQPSKYNFMPIVRGLVIA
                     IPIVMIFASLLASADVVFNQRLDDFVQIFKLENLPEYIFRMIYILVIGYALAGIFLHA
                     STESKDEKLVGEDKPVIPAFLGFIESSIVLGSVIALFAAFVVIQFQYFFGGTTNINVE
                     GYTYAEYARKGFGELVAVAFFALMMLLTLTGVTKRETETQRKTYSGLGIALVALLLVM
                     LVSAYQRLNLYEAAYGFSRLRTYTHVFLIWIGLLLITTIVLEILRKERMFTFAMLIAS
                     FGFAASLPILNVDAFIVNQNIQRELSGNDVEDLDSQYFIELSDDAVPPLVKALQTPSL
                     PDSVHEKVTAALACIRYQRDLQRNDREYSWQSFQFSRLNADNALKSVQAKIDVFELDE
                     SEYPTKAIAPSGEEYPCSQYYYD"
     gene            complement(4333..4677)
                     /locus_tag="PLG52_05040"
     CDS             complement(4333..4677)
                     /locus_tag="PLG52_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB40842.1"
                     /translation="MFPEITVTQLSEKLKSDEKFILLDVRELPELAQAKIEDSRLEVT
                     PMSRLGSEGPNALSESARSGEIPVYIMCHHGSRSAQVTMWLAQQGYKNIFNVRGGIDA
                     YARQVDSSVGFY"
     gene            complement(4749..>5132)
                     /locus_tag="PLG52_05045"
     CDS             complement(4749..>5132)
                     /locus_tag="PLG52_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan phosphorylase"
                     /protein_id="HNB40843.1"
                     /translation="VWMNTPRRPYEASGTSGMKAAINGVPNFSVMDGWWCEAYNGKNG
                     WKIGDEKKYASNEEQDAADAEDLYSTLEHQIIPKYYNRNPKDLMSPDWLAFMKDSMKT
                     VIPQFSTRRMVKEYVEKFYVPALKK"
BASE COUNT         1362 a         1130 c         1308 g         1332 t
ORIGIN      
        1 tgtgatgttt ggggaagagt cttttcatga tgataaagat ttggatacgg ctctttttct
       61 acaaaagctg aaggtggcaa agtcacttcc gaaaacggct gcccctgaac cttctctgta
      121 ttaccccatt ttagaagaag ctagaaaaaa tgatcaaagt gtggtcgttg tagctccaac
      181 tggcaaagcg agtggaactg tccgctcggc acagacggcg gcacaggaat ttccagaagt
      241 agatgtacgt attgtcgata cgcaaactat ctcctgtaac cttggttcac ttgtgttgtt
      301 ggcagatgat atggcgaagg ttggcaaatc agcgagtcag atcgttgcta aactagaaga
      361 tatgattcca cgtggaagaa tctattttct ggtcgataca ctagaatatc ttgccaaggg
      421 cgggcgcatc ggcggcgcca aacgtctcgt agcagagttg cttgaaatca aacccattct
      481 acagatcaaa gatgggcaag ttgaatcttt tgaacaacag cgaaccaaaa aacgtgcatt
      541 ggctcgtttg gtagagatta ccaaagaaca atgttccggt ggcgctgaaa gccatttgtg
      601 tgtgctgcaa gttgaggctg agaaagaagc gcaggcattg gttgaagaat tgaagtccac
      661 aatacctgtc gcagagattc ccatctacga attaccacca gctatcgtag tgcatgccgg
      721 tccgcgcgcg atggggattg gtttttttgt gtaatagaaa agtcgtcagt aaagtactga
      781 cgacttcttg ttttataatg cccgcactat gcttgaactt tccaataccc gctttgcatt
      841 tctggatgtc gaaactactg ggctttcacc ttggttcgga gatcgcatct gccaaatagc
      901 tgtgatcgtg accgagggca aacgcatcaa acggaccctt gacctgctcg ttgaccccga
      961 gcgtgagctt tcgccgtcag cgattcacgt caatggcttg gatgctgttg ccctcaaagg
     1021 aaaaccgcgc tttgctgaaa ttgcaaatga agtagaagaa gccctgaaag atgcagttgt
     1081 tgtttgccat aatgccaagt tcgatattca atttctggat aatgaatttc gcaaactcgg
     1141 gcggacgatt gaatttccca acttgattga cactttgcta ttagctcgcg attattacga
     1201 attgccttct tatagcctcg atcagctgtc acaggatttc catatctctg ccaacatgca
     1261 gggatcacac gccctagcag atgcgattgt gatgaagaat ctcttcttta ctatggcaga
     1321 ttcgctaaag acgatcgata agaccctcga tgacctgatt ggtatctata actcgcctgc
     1381 ctggcccgaa gaagggattt accttccaac tgaattaagc gaagctatcc atagtggaaa
     1441 gaaattattc attcaatata tggataaaga tggcgagata tcagagcgtt gggtttcgcc
     1501 acgcgaagtg attgggttgt cagattattt atacctacaa gcctattgcc actcccgtga
     1561 agcagatcgc acctttcggc tggatcgaat tttgagcgta gaagcggaga aataaataat
     1621 gcgacccgtt cgggtcgcat tatttttatg ctttgaagat ttgtgctaac gcgttcttga
     1681 acgcttcata tggctgcgca ccaacaatag cgtatttgtc gttgataaca taggtgggga
     1741 caccggttac gccgatttgt tgcgcccaat tgacctgccc agcaacctct ttagtgtact
     1801 tgccgccttc tacaaccgtt tgcatatcgt ctgggtctaa gccagcttct tctgctgtag
     1861 cgcggagcac atcccattga gctgggtcga agccatcagc atacaccttg cggaacatga
     1921 tgtgatggaa ctcattggct ttgccatgtt cgcgggcata ttctgtcgct tcgtgggcga
     1981 gacgtgtgtt gtaaattcgg tctgtagacc taaatggcat accgtgcatt tcagccatca
     2041 ttcgcaggcg ttcttccgag ccattgtcac gtgctcgttt gatgtaatca ggcaattcca
     2101 tgccttcggg cggagtatcg gggcgtaaat agtacggacg ccattccacc tccacgttat
     2161 attctttctt aagcctctcg accacaccct ggccgacata gcaccagggt catatgtaat
     2221 cggagaagat ggttagtttg aagtccattg gatgtccttt tgtgtgattg atgattgaca
     2281 gacattcaat gaaagagcat tcttacctaa cattaatcgt agtaatactg tgagcaggga
     2341 tattcttcgc cgcttggcgc aattgccttt gtggggtatt cagattcgtc gagttcgaat
     2401 acgtcaattt tggcttggac agatttcaga gcattatctg cattgaggcg cgaaaattgg
     2461 aaggactgcc atgaatactc gcggtcatta cgctgcaaat cgcgctggta gcggatgcag
     2521 gctagggcgg cggtcacttt ctcatggacg gagtcaggca gggacggagt ctgaagcgct
     2581 ttgacgagag gtggaacggc atcatcggag agttcaatga agtattgaga gtccaaatct
     2641 tctacgtcat ttccacttag ttcgcgttgg atattttggt tgacgataaa ggcatcgaca
     2701 ttcaagatgg gcagagaggc tgcaaagcca aacgatgcaa tcagcattgc aaaagtgaac
     2761 atacgctctt tgcgcaaaat ttcaagcacg atggtggtga tgagcagtaa gccgatccag
     2821 attaggaaga cgtgggtgta agtgcgcaag cgtgagaagc cataagcggc ttcgtagagg
     2881 ttgaggcgtt gatatgcaga gaccaacatc accagcaaaa gtgcaacgag tgcaatgcca
     2941 agcccagaat aagttttgcg ctgggtttct gtttcgcgtt ttgtgacgcc agtgagggtg
     3001 agcagcatca tcaaagcaaa aaatgctaca gctacgagtt cgccaaaacc tttacgggcg
     3061 tattcggcat aggtatagcc ttccacattg atgttggtcg tgccgccaaa gaaatactgg
     3121 aattgaatga ccacaaacgc tgcgaagagt gcgatgacac tgccaagtac gattgacgac
     3181 tcgatgaaac caaggaaagc agggatgacg ggtttatctt cgccgactag cttttcatct
     3241 tttgattcgg tggaggcatg gaggaaaata cctgccaagg cataaccaat gaccagaatg
     3301 tagatcatgc ggaagatgta ttcgggcaga ttctctagtt taaagatttg cacaaaatca
     3361 tcgagacgct ggttgaagac cacatcggca gatgccagaa gcgaggcgaa gatcatcacg
     3421 atcggaatgg caatcacaag tccgcgtacg atgggcatga aattgtattt ggaaggttgt
     3481 gtgcctgctt cggcttgggt tttacgcaca tcggcagtaa aggtgatggg gcgaataagc
     3541 atactgccga caagtgaaag taactttccg acataatctg ccaaagcata ttgaacccag
     3601 cgcccaccga gatatgtgac cgtgaaaatg gtcagggtga acatggtgaa ggtgtaagca
     3661 aggaaggtgg tcattggttc ggcgcggatg aaggtgacag cggcgaagaa gccaaagagt
     3721 ggaatcagca ataaggtcgt gcggtgcggg cgatgtccat tgctgagtaa gagatagaac
     3781 cctacgatga gacacgccgt ccaaaagatg gcgaagttga cgccaggagc ttgcttccag
     3841 aagaggaagt cgaatagcca gccaagggca aggacaaaga tccagagttt attagggtta
     3901 tttttcataa gggctccaaa ttatttttac cacgatatag ggaagcggta aaaagtttcc
     3961 ccgaggatag ccctgcaagg cttgcggaga ctctcaaaag gtatcagaaa tttgaaaatt
     4021 gtggcaaatt atagagagcg gcaaaggcga aaattacaga aatgtgagaa tcgaaaatcg
     4081 ccctattgac atccgatttg atttatgaca tacttttgct caccagacgg gggcaaacaa
     4141 attgaattcg ccatccttat gaaaggagga ggtgatgtct aacatggata gtagtagtaa
     4201 acccagcgct gtggcatccc tcctcaagta agtcaaagga agccacagcg ccccacgatt
     4261 gccacccgca agggtggcag tctggtttaa tagagatgaa taaaaaggtc acgcgaaaag
     4321 cgtgaccttt ttttagtaaa accctaccga gctatccacc tgccttgcgt aggcatcgat
     4381 ccctccgcgc acattgaaga tatttttata tccctgctgc gctaaccaca tggtgacctg
     4441 tgcactgcgg ctgccatgat gacacatgat gtagacgggg atttctcctg accgtgccga
     4501 ctctgataaa gcatttggac cttctgaacc taggcggctc attggcgtga cctcaagcct
     4561 gctgtcttcg atcttggctt gagccaactc tgggagttcg cgcacatcca acagaatgaa
     4621 cttctcatcc gatttcagtt tttcactgag ttgggtaacg gtaatttcag ggaacatgtt
     4681 tatttcctta ataatgtagt ggcggggtaa taccacttcg tggcacaagc cccaccacta
     4741 tattgatatt acttcttcaa tgctggcaca tagaactttt ctacatattc cttcaccata
     4801 cggcgagtgg agaattgtgg gatgacggtc ttcatcgaat ctttcatgaa ggcgagccaa
     4861 tctggtgaca tcagatcttt ggggttgcgg ttgtagtatt tcggaatgat ctgatgttca
     4921 agtgtggagt acaggtcttc tgcatcagca gcatcctgtt cttcgttcga ggcatatttc
     4981 ttttcatcgc cgatcttcca accgttcttg ccgttatatg cttcgcacca ccaaccgtcc
     5041 atgaccgaga agttgggcac gccgttgatg gcggctttca tgccggaggt tccactcgct
     5101 tcgtaagggc ggcgcggagt attcatccac ac
//
LOCUS       DAOQNJ010000126         5117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9825_length_5117_cov_7.448044, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000126 DAOQNJ010000000
VERSION     DAOQNJ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9825_length_5117_cov_7.448044"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PLG52_05050"
     CDS             complement(<1..1119)
                     /locus_tag="PLG52_05050"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNB40844.1"
                     /translation="MLQRLQVQLVILLTTFVLLVGVSASLTFWGLQTQQQDALVINLA
                     GRQRMLIQQMTRLALQLQGGDESALGELRESERMFGETLSALQNGGEAPYLEEGIASL
                     PATRDPQILAALQDMESNWLQYRSMLGTIAASPSESGLLQDDLGIQSKILVQKTDAVV
                     RLYEAASIAKVNRLRLIQFVFLISALALLAVGAWITRRSLLKPLLALGVAAKRLGKNQ
                     LDGAVQVQGPEEMRVLSQAFDDMRLSLQSARVELIQWNAELEQRVAQRTQELETLNQV
                     SREISSRLDIQQVLNSVTEKARILLGGEVASLCLVDENQHWLKLQALSGPQHAIVGKV
                     MPTDEQFALAVLASDHTMICGSESCNGGCRMLSDEYRAS"
     gene            1721..3093
                     /locus_tag="PLG52_05055"
                     /pseudo
     CDS             1721..3093
                     /locus_tag="PLG52_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611865.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3142..3543)
                     /locus_tag="PLG52_05060"
     CDS             complement(3142..3543)
                     /locus_tag="PLG52_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40845.1"
                     /translation="MKNQASTSKKVTAKHRQKKEYCKWFVFIGTWKNIGWGTGWSSHW
                     EAAQIALPCSPCSMTPPEINSMLSCAWGEDRLYCGIRAMLTVFEIVHNYINIIMLVKE
                     NFDSNIAPIRAWVAQMELDNMKKKIVWCQKK"
     gene            3758..3988
                     /locus_tag="PLG52_05065"
     CDS             3758..3988
                     /locus_tag="PLG52_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNB40846.1"
                     /translation="MKKILFVINDAPYGDERPYNAMRHAMNLVKRDEVQVMVFLVADS
                     VYCARKAQKTPDGYYNIERMVKSLAKRGKVAT"
     gene            complement(4135..4746)
                     /locus_tag="PLG52_05070"
     CDS             complement(4135..4746)
                     /locus_tag="PLG52_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB40847.1"
                     /translation="MPRKSSPVNIDPRIKRTRRLLRDALVSLILKKDFASITIREVTE
                     RAEVAYITFYRHFESLDQLLMEVLDEGLVELMSHIEALAKLSDPSSLETEGRLIFEYV
                     GQKADLFRILLKSQSVTRVRKKVVSNISAIFQKSCVPLERLGNQVTINMASNHIATSL
                     LSLIEWWLENNMKPSPIEMGKVYKSLIIDSTVGAVSSLSAVAK"
     gene            4903..>5117
                     /locus_tag="PLG52_05075"
     CDS             4903..>5117
                     /locus_tag="PLG52_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40848.1"
                     /translation="MKTITTQEGKTCKPQRIPLGAKLTASQIQNAYDEIASQYEKRVW
                     FDQHILGVARLKKKLLSKANGKILEVA"
BASE COUNT         1410 a         1256 c         1212 g         1239 t
ORIGIN      
        1 gctggcgcga tattcatccg aaagcatacg gcatccgcca ttgcaggatt ccgaaccgca
       61 gatcatggtg tggtcacttg ccagcaccgc cagtgcaaat tgctcatcgg tcggcatcac
      121 cttgcccaca atcgcatgtt gcggtccgct caatgcctga agtttcagcc aatgctggtt
      181 ttcatcgacg aggcacaacg aagccacctc gccgcccaac aagatgcgcg ccttttcggt
      241 aacagagttc aacacctgtt gaatgtcgag ccgcgacgaa atctcacggc tgacttgatt
      301 cagcgtttca agttcctgcg tgcgctgggc aacacgctgc tccaactctg cgttccattg
      361 gatgagttcg acgcgggcgg attgaagact gagccgcata tcgtcgaacg cctgcgacaa
      421 aacccgcatc tcttcgggac cctgaacctg caccgcccca tcgagctgat tcttcccgag
      481 tctttttgcc gccacgccta acgccagaag cggcttcaac agggaccgcc gcgtaatcca
      541 cgcgccgacc gccagcagcg ccagcgcgga aattaaaaat acaaattgaa tcaggcgcag
      601 gcggttcacc tttgcaatcg aagcggcttc gtacaaacgc acgaccgcgt ctgttttttg
      661 aaccagaatt tttgattgga ttccaaggtc gtcttgcagc aaacccgact cggacggcga
      721 agcggcaatg gttcccaaca tggaacggta ttgaagccag ttcgattcca tatcttgcaa
      781 cgcggctaaa atctgcgggt cgcgcgtcgc tggcaagctg gcgataccct cctccaggta
      841 tggagcctct cccccgtttt gcaaagcgga taaagtttcg ccgaacatcc gctccgactc
      901 gcgcaattcc cccagcgcgg actcatcccc gccctgcaat tgaagcgcaa ggcgggtcat
      961 ctgctggatc aacatccgct gacgccccgc aaggttgatt acaagcgcgt cctgttgctg
     1021 agtctgcaaa ccccaaaagg tcaggctcgc agaaacaccg acaagcaaaa cgaatgtggt
     1081 gagcaggatg accagttgaa cttgaagacg ctgtaacatg gggttggcga aaaatccttg
     1141 cgagaggatt tttcaattat acctgctacg cgttaagaat ctggcgggag gcaagagcgc
     1201 tctttggagt ctggtgtggt ttccgttcaa tggaaagaaa aaagccgccc tttgcagatg
     1261 tcattgaaaa gtggaaagcg ccgcatttta atacattgac caaataagac tttttcacaa
     1321 tcgcaatcgg atctgtccgt cggggtacat atctggtata cgggtataat ttcgccccac
     1381 attgaaccac atttaataca taccacaccc tgcccccata tatggagata gggtgccaag
     1441 aacagaataa aactctccat atagctgaat gatcgtatac ggaaatggaa tttctctcat
     1501 aaacgatcat gccgcactgc tctacttggg agtgcgtact taatgaaaaa aatgctttga
     1561 taatcctttc tcaggattgt caaagcgtta tccttttata agctgctcat tacagccgag
     1621 ctatgtatgg tgtcagaaga aatgagacac aggggcggaa aaagtaaggt ggaaatcccg
     1681 gccaaaatct gattaaatca gaaaacccaa ggagtagaaa atggccgaag aaaaagtctc
     1741 atccgtggaa gccacagtcc gggagattcg gcggaagacc cgcaagaagt acaatgcgga
     1801 agaaaaaatg cggatcgtgc tggaaggatt acgtggggaa accagcatag ccgaattatg
     1861 tcgtcgcgaa ggactgcatc ccaatctata ttacaaatgg agcaaagagt tccttgaagc
     1921 aggtaaacaa cgattagtgg gagacatcca tcgcgaagca gacagccgtg aagtggatga
     1981 aatgcgcagc gagattgaac aacttaagca gttggtagca gaactgtcac tcaagaatcg
     2041 agtgctaaaa aaaagcttgc tgggcaagga cacttcatgg gaggattaag cgtacctgtt
     2101 gcgaacatta gtatgcgaca cagccagata gagaaacagg agatcatcca tctggtggaa
     2161 cactcgcaat tgtcagtcag gaaaaccctg gaagaattaa acgttcctgc cagcaccttc
     2221 tatcgctggt accgaaagta cgaggaagat ggcgtggatg gcttgatcga ccgcaaccca
     2281 agtccgcgcc aattctggaa tcgtattcct gaggcggtcc gcaagcaggt ggtggatctg
     2341 gcattggacc atccagacca ctcttcaagg cagattgcct ggctttttac cgaccaacag
     2401 ggctatttca tctcggaatc aagcgtgttt cggattctga agcgctttga tctggtggag
     2461 agtcccgcct tccgagtatt gagcgcagca gacaaattcg tgcaccctac taaacgggtg
     2521 aacgagttgt ggcagacaga cttcacttat ttcaagatct tgaactgggg ctggtactac
     2581 ctatctactg tgctggatga ttattcccgc tacattctgg cttggaaatt gttccccacc
     2641 atgtcagcca atgatgttca ggttacgctt cagatcgccc tcgacaagac tggcttggat
     2701 caggtcttgg tggaacatcg tccgcggctg ttatcagata atggttcttg ttatctgtcc
     2761 aaagacttaa aggatttttt ggatcggaag cacatggaac acacacgtgg cgcgccttat
     2821 caccctatga ctcagggcaa gatcgaacgc tatcatcgct ccatgaagaa cattgtgaac
     2881 ttgcagaact acttcctgcc ggaggaattg gaacgggaag tcgcatcatt tgtggagtat
     2941 tacaaccacc agagatatca tgagtctctg gacaatttaa ctccggcaga tatgttttat
     3001 ggcagagcca aggaggtact aacccgacga caggagatca agctaagaac gttacaggaa
     3061 cgacgcttgc agaacctgcg agctgctgta taatcccaac catccgggaa atctacacct
     3121 taactattcg ctctttgtgt ctcatttctt ctgacaccat acaatctttt ttttcatatt
     3181 gtctaattcc atctgagcta cccaggcacg gatgggagcg atgtttgaat caaagttttc
     3241 ttttaccaac atgatgatgt ttatatagtt atgtacaatt tcaaagactg tcaacatggc
     3301 tcgtatcccg caatacagtc tgtcctctcc ccaggcgcag gataacatcg aatttatttc
     3361 tggcggagtc attgagcatg gactgcaagg cagggcgatc tgagcggctt cccaatggct
     3421 cgaccagcct gttccccacc cgatattttt ccacgtccca ataaacacga accacttgca
     3481 atactctttc ttttgcctat gtttggcagt cactttcttg ctcgttgagg cttgattttt
     3541 caccttgggt ttcggatgat gtacggatat agatcgctgc tcggcgagac atattggact
     3601 ccatggtcat atttattacc tgccacttct aatacgccga aaacgcgaac ttggcagagg
     3661 cccagctacg ggcttcaagt tataatttca tcatgaatgc aaaatctcaa tgggaaacca
     3721 ttcgccaaac caatacccaa caaaggattg atatcaaatg aaaaagattc tatttgtgat
     3781 taatgatgcc ccctatggag atgagcgtcc ctataatgcc atgcgtcatg cgatgaatct
     3841 ggtcaaacgc gatgaagtac aggtaatggt cttcctggtt gccgattcgg tctattgtgc
     3901 tcgcaaggcg cagaaaacac cagatggtta ttacaacatc gagcgcatgg tcaagtcact
     3961 cgcaaagcgc ggcaaggtag ctacctgagg cacatgttgt gaagcccgag gtctcgggtt
     4021 ggatgaatta acagaaggag tcgagatggg gaatatggat ttactaggtg attggacatt
     4081 ggaagcagat caggtgattg tgtattaatt gaaaatatga tgcgggtaaa acacctattt
     4141 tgccactgcc gataaagaac ttaccgctcc taccgttgaa tcaataatca aactcttgta
     4201 aaccttcccc atctcaatcg gggaaggttt catattattt tccagccacc actcgatcag
     4261 tgacaataaa gatgtggcga tgtggttcga tgccatattg atcgttacct gattacccag
     4321 cctttccaat ggcacacaag atttctggaa aatggcggat atattactca ccaccttctt
     4381 acggacgcgt gtcacactct ggcttttgag caggatgcga aagagatcgg ctttctgccc
     4441 aacgtactca aagataagtc tcccctccgt ttcaagggac gatgggtcag acagtttcgc
     4501 aagcgcctca atatgggaca tcaactctac cagcccttcg tccaacactt ccatcagcaa
     4561 ctggtcgaga ctttcaaaat gcctatagaa cgtaatgtaa gctacctcgg cacgttctgt
     4621 gacctccctg attgtgatgg aagcgaagtc ctttttcaga atcaatgaaa ccagcgcgtc
     4681 gcgcaacaaa cggcgtgtgc gtttgattcg agggtcaatg tttacggggg atgattttct
     4741 tggcatggtt atccagtttt acttcgacaa gctcagtaca agtgttacaa aacatagctt
     4801 tctataactt aagttacatc aacaccacat ccgcaattga cttgctcata gggctggcta
     4861 caatacacat actgttacac agtataacaa aaaggagcaa tcatgaaaac gataacaaca
     4921 caggaaggaa aaacctgcaa gccccaacgc atccctcttg gcgcaaaatt gacggcgtct
     4981 caaatacaaa atgcctatga tgagatcgcc agccaatatg agaaaagagt ctggttcgac
     5041 cagcacattc ttggcgtggc tcggctgaaa aagaagctac tttcaaaagc aaatgggaaa
     5101 attttggaag tggcgtg
//
LOCUS       DAOQNJ010000127         5088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_9897_length_5088_cov_9.501291, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000127 DAOQNJ010000000
VERSION     DAOQNJ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9897_length_5088_cov_9.501291"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="PLG52_05080"
     CDS             complement(<1..1142)
                     /locus_tag="PLG52_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNB40849.1"
                     /translation="MPPTLYLIDGHALAYRMYFALTAGGGSQRWQTSKGEPTAGIYGF
                     ARELVRVLEQEKPEYLAVAFDVGKTFRDQIFPEYKATREKMPDDLRSQIARIREMVDA
                     FNIPRLEMDGYEADDVLGSVAKLAAEKGLGVKIITGDRDLLQLVNERTAVYLAGDDQT
                     YITDADVIKKLGVRPNQVVDYKAIVGDTSDNIPGVKGVGEKTAVALLEKFNTLDAIYA
                     NLDQVENRWRGKFETNKETAYMSRDLAQIETNLKIKFDFEHAKVAPFDGSKLEAFFKE
                     MEFRTLVSKVPAISGGVASASAETKAPTVKTGKTKAGQMTMFAEEAQPVAVAIKPASI
                     EVVIVDSAEKLKSLANELEKAKVISFDTETTGTEEMTAELVGISLAV"
     gene            complement(1249..2919)
                     /locus_tag="PLG52_05085"
     CDS             complement(1249..2919)
                     /locus_tag="PLG52_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNB40850.1"
                     /translation="MPADLMVVSPSSSLGEAIRRSLEDTKFYRVTTVNNKAAAIVKAN
                     EIGMPMAMLDLALGEEWVAEIGASLRTIRPSINLVILCEEAGSPPAFDSLRPWIMVRK
                     PFKISDFMNAISQPQSAQPAKSGAPAPAMPMSSTAQFMPWLNDPNKAAQHLTRLTLES
                     SAQAALITKKNDLWAYAGGLSQNAAKEVAQTVTRNWDGQKGSDLLRFIRLESTKAEHM
                     LYATRLTSDAVLALVFDAETPFSTIRSQASQLLSDFGSEKAEPKNQPAVSYAVSDDDS
                     KDEDLNIPPISNILADIPAPNPEPLTTRDFNLPRKTPVQPQPQEAFDPNQTRVSDALS
                     NASVFSRDPSPAVRRDSVPQPMAQTQKSEAHHDTQIDFDEVQVTAPSQPKARPETPVA
                     RPRPGENDATRESPSTEMARKLTTEPISAGLYHLTYACLLVPRFSSHYLTGDLSDRLG
                     EWLPNICIAFGWRLEFLAVRPEYLQWVVNVQPNTSPGHLMRIMRQQTSEKIFGDFPRM
                     KRENPSGDFWAPGYLIMGGSQPHPPQLVRDYIRQTRQRQGLEDLPPMK"
     gene            complement(3006..3569)
                     /locus_tag="PLG52_05090"
     CDS             complement(3006..3569)
                     /locus_tag="PLG52_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40851.1"
                     /translation="MLKPTDLILVCLMPTPRDMEIARLLGWYRIPLKSAPKVVSVDYL
                     AFYQPASFGERGGQVEYIAQVRGHELTTRGELLKDEADHPRAKEEYFKLQLGNLEKLK
                     EPVRTDKWKRLTFLYSTGEYLLNAKTLNDLVVEGEERNMLWKNLRERAENDQLYQVDL
                     PEADIPPEVLMALLGIKDLAGGYDAGQ"
     gene            3591..4589
                     /locus_tag="PLG52_05095"
     CDS             3591..4589
                     /locus_tag="PLG52_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNB40852.1"
                     /translation="MRLSFLLLSCSLLVACGGGATPLPAPEVDSTPIPSSTPQPTATS
                     APVPNFPVIGYLPDYRELNLAWASSLTDIIYFSAEPRADGSLDTSRLTEETWQALASL
                     KDENGIRVHISIGGWGRDGAFVEMSADPETRKTFIANLLNFLQERQLDGADFDWEFPQ
                     NDTEFEQYIALLTETKAALSPHGLLVSVALPAESSFPLGEFAVVDRVHLMSYDRGERH
                     STFEQSVADVEAFLEAGIPPEKLILGMPFYGRGMSSPSDTYSYEQIRSTYAPAPDVDE
                     VGDIYFNGLNTIQHKVCYGIAQNLGGFMVWELGLDTVDESSLLQQIYRLAQGAESC"
     gene            4583..>5088
                     /locus_tag="PLG52_05100"
     CDS             4583..>5088
                     /locus_tag="PLG52_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40853.1"
                     /translation="MLIHPSPYERITALTDIFLGILAAWGAAQLFAFADFRHQIWAWT
                     FCLLAIASFLGAIAHGFVMSTKTNDRLWMPINVALGLTLGLFVVGALFDLSGESVARQ
                     ALPVMIALGFGFFLITILKPGTFMTFIVYEALAMLFALGIYGYLFFTSNLLGVGWMVL
                     GVLVTIIAA"
BASE COUNT         1098 a         1283 c         1341 g         1366 t
ORIGIN      
        1 acagccagtg aaatgccaac caattcagcc gtcatttctt cggtgcctgt cgtttccgta
       61 tcgaacgaaa tgaccttggc tttctccaac tcgttggcga ggctcttcag tttctcggcg
      121 ctatccacaa tcacaacttc aatgctcgcc ggtttgatcg ccaccgccac gggttgcgcc
      181 tcttctgcga acatggtcat ctgcccggct ttggttttgc cggttttcac agtgggggct
      241 ttggtctctg ctgaggcaga agccacaccg ccgctaatgg caggcacttt gcttaccaga
      301 gttctgaatt ccatttcttt gaagaaagct tccaacttcg agccgtcgaa gggtgccacc
      361 ttggcatgtt caaaatcgaa tttgatcttg aggttcgttt caatctgcgc caggtcacgg
      421 ctcatatacg ccgtttcttt gttcgtctcg aatttaccgc gccagcgatt ttccacttgg
      481 tctaagttgg catagatcgc atccagcgtg ttgaactttt ccagcaaggc aaccgctgtc
      541 ttttcgccca cgccctttac gccgggaata ttgtccgacg tatcgccgac gatggcttta
      601 tagtccacta cttgattcgg gcggacgccc agctttttga tgacatcggc atcggtgatg
      661 tacgtttggt cgtcacctgc cagatacacc gccgtccgct cattgacaag ctgtagcagg
      721 tctctgtctc cggtaatgat tttcacgccg agaccttttt ccgccgcgag ctttgccaca
      781 ctgcccagca catcatcggc ttcgtagccg tccatttcga ggcgcggaat attgaacgca
      841 tccaccattt cacgaatgcg cgcgatctgc gaacgcaaat catcgggcat cttttcgcgg
      901 gttgctttat attccgggaa gatctggtcg cggaaggttt tgcctacgtc gaaagcaacg
      961 gcaaggtatt caggtttttc ctgctccaac acgcgcacca gttcacgcgc aaaaccgtaa
     1021 ataccagccg tcggttcacc tttcgaagtc tgccaacgtt ggctgcctcc gcctgctgtc
     1081 agggcgaagt acatgcggta ggcgagagcg tgtccatcaa tgagataaag ggtagggggc
     1141 atgtcagtat ccagtcgtgt agtgaccaat tggaatagtg tctaaaagaa aaaacgcaga
     1201 gttaattata ctctgcgttt ccttatttag aacttttgtt tcagaaacct acttcatagg
     1261 tggcaggtct tccagacctt ggcgctgacg ggtttggcga atgtaatcgc gcaccagttg
     1321 tggcgggtgc ggttgtgaac cgcccatgat gagataaccc ggtgcccaga aatcaccaga
     1381 ggggttttca cgcttcatgc gcgggaaatc gccaaagatt ttttctgaag tctgctggcg
     1441 cataatgcgc atcaagtgac cgggcgatgt attcggctgc acgttcacaa cccattgcaa
     1501 atattccggg cgcacggcta agaactccaa gcgccagccg aaagcgatgc aaatgttagg
     1561 cagccattcg ccaaggcggt ctgagagatc gccggtcagg taatgggagg agaagcgcgg
     1621 caccaacaaa caagcgtaag tcaggtgata caagccagcc gagatcggct ctgtggtcag
     1681 tttgcgcgcc atctcggtag aggggctttc gcgagtggcg tcattctcgc cagggcgtgg
     1741 acgtgctacc ggggtttcag gtcgcgcctt gggctgagaa ggagccgtca cttgcacttc
     1801 atcgaaatcg atttgcgtat cgtgatgggc ttcagacttt tgagtctgtg ccatgggctg
     1861 cggcaccgaa tctctgcgaa cagcaggcga tgggtcgcga ctaaagaccg aagcgttgga
     1921 aagcgcatcc gaaacgcggg tttgattcgg gtcgaatgct tcttgcggtt gaggttgcac
     1981 aggggtcttg cgcggtaagt tgaaatcgcg tgtggtaagt ggctcagggt tcggagccgg
     2041 aatatccgcg agaatgttcg agatgggggg gatgtttaag tcttcatcct tgctatcatc
     2101 atcgctgaca gcataggaga cggcgggttg atttttgggc tccgctttct cactgccaaa
     2161 atcgctcagc aattggcttg cctgagagcg gatggtgcta aagggagttt cggcgtcgaa
     2221 aacaagagcc agcacggcgt ctgaagtgag gcgcgtggcg tagagcatgt gctccgcttt
     2281 ggtgctttcg agtcgaataa agcgcagaag gtcagaacct ttttgtccgt cccaattgcg
     2341 ggtgacggtt tgcgctactt ctttggcggc gttttgagag agaccgcctg catatgccca
     2401 caagtcattt ttcttggtga tcagcgccgc ttgcgccgaa gattccaagg tgagacgggt
     2461 aaggtgttga gccgctttgt tggggtcatt cagccacggc atgaactgcg ccgtgctcga
     2521 catcggcatg gctggagccg gagcgccaga ctttgctggt tgagcagatt gaggttgact
     2581 gatggcattc ataaaatcag agattttgaa cggtttgcga accatgatcc atggacgtaa
     2641 tgaatcaaag gcaggcgggg agcctgcttc ttcgcagagg ataacaaggt tgatgctggg
     2701 gcgaatggtg cgtaaagatg cgccaatttc tgcaacccac tcttcgccaa gtgcaaggtc
     2761 gagcatcgcc atcggcattc cgatctcatt tgccttgacg atcgctgcag ccttgttgtt
     2821 gacggtagtg actcggtaga acttggtgtc ttccagcgaa cggcgaatgg cttcaccgag
     2881 gctactagaa ggggaaacaa ccattaaatc agcaggcatc ggttaaagtg tatcacttct
     2941 gttatacgca tgcaaggaaa cctttgggtg gtttcttatc attttagtaa ctcttctgga
     3001 caactttact gtcccgcgtc gtagccgcct gccaggtctt tgatgcccaa aagcgccatc
     3061 agcacctctg gcggaatatc tgcctcgggc aaatccactt gataaagctg gtcgttttcg
     3121 gctcgctcgc gcaggttctt ccaaagcatg ttccgttctt cgccctctac caccaagtca
     3181 ttcaaggttt tagcgttgag caaatattcg ccagtggaat acaagaaggt cagccgtttc
     3241 catttatcgg ttcgaaccgg ctctttcaac ttctccaaat ttcccaactg caacttgaaa
     3301 tactcttctt ttgctcttgg gtgatcggct tcatccttga gcaattcgcc tctggttgtc
     3361 aactcatgcc cacgaacttg agcgatgtat tcaacttgtc cgcctctttc gccgaatgaa
     3421 gcgggttggt aaaacgcaag ataatccacg ctgacaactt tcggcgcgct cttcaatggg
     3481 atgcgatacc accccagcaa acgcgcaatt tccatatcgc gcggtgtagg catcaggcaa
     3541 acaaggatca agtccgtagg tttgagcatg gcttaattat aatgttcacc atgcgactct
     3601 cattcctcct tctatcatgt tctctactag ttgcttgtgg aggaggggct acgcctcttc
     3661 ccgcgcccga agtggattca actcccatac cctcgtccac gccccaaccg accgccacct
     3721 cagcgcctgt gccaaatttc cccgtcatcg gctatttgcc agattatcgc gagctgaacc
     3781 tcgcatgggc atcttccttg accgatatca tctacttctc tgccgagccc cgcgccgatg
     3841 gctcactgga tacttcacgc ttaacagaag agacatggca ggcattggca tcgttaaagg
     3901 atgaaaacgg gattcgcgta catatctcca ttggcggctg ggggcgagat ggtgcctttg
     3961 tagaaatgag cgccgaccct gaaactcgta aaacctttat tgctaacttg ttgaactttc
     4021 tacaagagcg tcagcttgac ggtgccgact tcgattggga atttccacaa aacgacacag
     4081 agtttgaaca gtacatagcc ttattaaccg aaactaaagc tgctctttcc ccgcatggct
     4141 tgttggtgag tgttgccctc ccagctgagt cgagtttccc gcttggtgag tttgcggttg
     4201 ttgatcgtgt gcatcttatg tcctatgacc gcggtgaacg acactccacc tttgagcaat
     4261 ccgtggcaga tgtggaggct tttttagaag caggtattcc acctgagaag ttgatcttag
     4321 gcatgccttt ctatgggcgt ggaatgtctt cgccatctga tacctattct tatgaacaga
     4381 tacggtctac ttatgcgcct gcaccagacg tggatgaagt gggggatatt tatttcaacg
     4441 gattgaacac cattcagcac aaagtttgtt atggcatcgc gcaaaacttg ggcggattca
     4501 tggtttggga gttagggcta gatacagtgg atgagagttc attactccaa cagatatatc
     4561 gcttggcaca aggagcagag tcatgttaat tcatccttcc ccgtatgaac gtatcactgc
     4621 attgaccgat atctttttag ggattctggc agcatggggt gcggctcaac tttttgcctt
     4681 cgccgatttc cgccatcaaa tttgggcatg gaccttttgt ttgctggcaa tcgcctcttt
     4741 tctcggtgcc atcgcccacg gatttgttat gtcaaccaaa acaaatgacc ggttatggat
     4801 gccgattaat gtagcgctag ggttgacctt aggcttgttt gttgtggggg cattgtttga
     4861 cctaagtggt gaaagcgtgg ctcgccaagc gttgcctgtg atgatcgcgc taggcttcgg
     4921 ctttttcctc attactatct tgaagcccgg aacttttatg accttcatcg tctatgaagc
     4981 gcttgctatg ctctttgcat tgggcatata cggctattta ttttttacat ctaatttgct
     5041 gggtgtaggg tggatggtgc ttggggtgtt ggtgacgatc atcgctgc
//
LOCUS       DAOQNJ010000128         5047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10028_length_5047_cov_8.464744, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000128 DAOQNJ010000000
VERSION     DAOQNJ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10028_length_5047_cov_8.464744"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLG52_05105"
     CDS             complement(<1..740)
                     /locus_tag="PLG52_05105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB40854.1"
                     /translation="MKIGLVIYGSLDTLSGGYMYDRMLVEHLRAQGDTVEIISLPWRN
                     YAAHLTDNFTFKLPSGLDVLIQDELNHPSLILANRHPHPYPIISLVHHLRCSELRPQW
                     QNDFYRVVEKKFLQSVDGFIFNSETTKGVVNRLIGNSSPEIVAFPPTDRFGEAIAEDG
                     IKERSQEKELNILFLGNIIERKGLHTLLQAAKLLTCDFRLDIVGGLAAEPEYAKSVQA
                     YIADNNLSSVISLHGALNNQPLVDKLKQA"
     gene            complement(741..1118)
                     /locus_tag="PLG52_05110"
     CDS             complement(741..1118)
                     /locus_tag="PLG52_05110"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNB40855.1"
                     /translation="MYSLGVRREFIARHFLIGGDWGPENFPNSHHYTLELVLEGKELD
                     QHGYLVDIVDVEKHLDEIVNYYKEQMLNEKPEFAGLNPSIEHFSRILATTLSERIKAK
                     NISGLKVVLWEHANAWAAFQVTR"
     gene            complement(1145..1819)
                     /locus_tag="PLG52_05115"
     CDS             complement(1145..1819)
                     /locus_tag="PLG52_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNB40856.1"
                     /translation="MKFRRYTLDDCHQATGIVLVIDVLRAFSTAAYAFSRNAKEIRLV
                     STVEEALSLKAQISNSKAIGEVGGIPPEGFDFGNSPTHILEHDLTGITLIQRTGSGTQ
                     GAVRAVNANVLLATSFVVSGATIEYVSKLQPHEVSFVITGGMDNDEDVACADYLEMKF
                     TGQQSNYEIFIRRVYESKDALQHRQEHPQFPYSDLDYCSRINYFDFAMPIERKDGFLI
                     TQAVKP"
     gene            complement(1816..2148)
                     /locus_tag="PLG52_05120"
     CDS             complement(1816..2148)
                     /locus_tag="PLG52_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40857.1"
                     /translation="MENKPASENGFSNSFIGKLFLGGLPVLGGLLTILVSLNILPYTD
                     LRPNRIAIFNDPHTWEVFAVGATMLFFGIANILPPQMKALGRINTFLFFLSLMAVVIG
                     VLLKRFSG"
     gene            complement(2138..3133)
                     /locus_tag="PLG52_05125"
     CDS             complement(2138..3133)
                     /locus_tag="PLG52_05125"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNB40858.1"
                     /translation="MFPAKTLFFTAPRQVEVRESNLPLLKEDEVLVETICSAISAGTE
                     MLVYRDQFPQLKDSHDAVSSELKYPLAYGYACVGTVREIGKVVGKEWQDRLVFSFQPH
                     TSHFLSKPENLFPIPNSLKPENACFLPNMETAVNLVQDGAPILGERVLVLGQGIVGLL
                     TASLLHEFPLENLTVVDAIELRRKAMKEIEGQMSKVNKLSPSDLQSSSFDLVFELTGS
                     PSALNTAIEHTSFSGRIVIGSWYGQKRAEIDLGGSFHRSRIQLISSQVSSISPTLSGR
                     WDKSRRFEVAWQALERIQPQKWITHRFSLNEAANAYRLLDENSHETIQVVFDYGK"
     gene            complement(3190..3579)
                     /locus_tag="PLG52_05130"
     CDS             complement(3190..3579)
                     /locus_tag="PLG52_05130"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNB40859.1"
                     /translation="MQERRKEERKNLVAYTQVYDLYGGNLLGYLGDLTISGAMIISEK
                     AMKPDTEITLAIELPELPGIKAIRMSLLTRVAWCEQDLSPQYFNIGFEFKEITPDQKG
                     LIEAIIKNYEFRRDVPKYPTRGAPPKQ"
     gene            3656..4381
                     /locus_tag="PLG52_05135"
     CDS             3656..4381
                     /locus_tag="PLG52_05135"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNB40860.1"
                     /translation="MRWIYLSPHLDDAALSAGGWIYDQTQAGNKVEIWTFLCGYPPSK
                     ELSMFARILHMQWGMPTARQVIRMRRAEDKAAATILGAKVRHFNFLDCIYRRDVEGNW
                     LYTDVFVDPHPSEANLPAEIASAISARLKPDDKLICQFGVGKHVDHVTVRRAAELLGR
                     PLHYVTDIPYYFNTPEHLAPNTVGMKKTVERVSRDGLASWKEAILAYKSQISSLFKSQ
                     RAMRAQINQYCSEFKGMPFWTTN"
     gene            4524..4994
                     /gene="greA"
                     /locus_tag="PLG52_05140"
     CDS             4524..4994
                     /gene="greA"
                     /locus_tag="PLG52_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNB40861.1"
                     /translation="MPTSFLTKEGYDKLQEELDHLRTVKRQEVAERLHEAMEGGELIE
                     NAEYEAAKNEQAFVEGRIQELDLLLATAQIIEDNGKGKKHDAIQVGSKVTIKEGNYEA
                     ETFTIVGAAEANPREGKISNESPIGKAILGHKVGDTVKVETPGGTYNVKILKVA"
BASE COUNT         1368 a         1139 c         1206 g         1334 t
ORIGIN      
        1 gcttgtttga gtttgtcgac aagaggttga ttgttgagtg caccgtgaag agagataacc
       61 gatgaaagat tattatcggc gatgtaagct tggacagatt tagcgtattc tggttcagct
      121 gccagcccac cgacaatgtc gagccggaag tcgcaagtca aaagtttagc ggcttgcaac
      181 agagtgtgaa gtcctttgcg ttcaatgata ttgccaagaa aaagaatgtt cagttctttt
      241 tcttggcttc tttcttttat accgtcttct gcaattgctt ctccaaaccg atctgtcggt
      301 ggaaatgcta cgatttctgg cgagctattc ccaattaacc gattcaccac gccctttgtg
      361 gtttcagagt tgaagatgaa tccatccacg ctttgcagga attttttctc cactacacga
      421 taaaagtcgt tctgccattg cgggcggagt tctgaacagc gcaggtgatg taccaagctg
      481 ataatgggat aggggtgtgg atgtcgattc gcaaggataa gtgaaggatg gttcagttca
      541 tcttgaatca aaacatccaa gccagaagga agcttgaaag tgaaattatc cgtcagatgt
      601 gcagcatagt tccgccaggg gagcgagata atctccactg tatcgccctg ggcacggaga
      661 tgctccacca acatgcggtc atacatgtat ccgccgctga gcgtatccag tgagccgtaa
      721 atgacaagac cgattttcat ttagcgtgtt acttgaaacg ctgcccaggc attggcatgt
      781 tcccacagaa ctacttttaa accagagata tttttagctt tgatgcgttc actcagggtt
      841 gtagcaagaa tccgtgagaa gtgttcaatg gatgggttca gtccggcaaa ttcaggtttc
      901 tcattcaaca tttgctcttt gtaataattc acgatctcat cgagatgttt ttctacatct
      961 acaatgtcta caagatagcc gtgttgatca agttctttcc cttccagaac aagttccagc
     1021 gtgtagtgat gcgagtttgg gaagttttct ggtccccaat ctccgccaat caaaaagtga
     1081 cgtgcaatga attcgcgtcg tacgccaagt gaatacatag tgttctccaa agtaaaagtc
     1141 aggcttaggg tttaactgcc tgcgttatta aaaatccatc cttacgttcg atgggcattg
     1201 caaagtcaaa atagttgatg cgtgaacagt agtctaagtc agaatatgga aattgaggat
     1261 gttcctgcct gtgttgaagg gcatcttttg actcgtaaac acggcggatg aatatttcat
     1321 agttagattg ttgacctgtg aatttcattt ccaaatagtc ggcgcaagca acatcttcgt
     1381 cattgtccat tccacctgta atgacaaagc tgacttcatg aggttgtaat ttactcacat
     1441 attcaatcgt ggctcctgac accacaaaac tggtcgccaa taaaacattt gcattcacgg
     1501 cgcggacagc cccttgtgtg ccagagcctg tgcgctggat gagagtgata cctgttaggt
     1561 catgttcgag aatgtgagta ggggagttgc caaagtcgaa gccttccggt ggaatgccgc
     1621 ctacttcacc gatagctttt gagttggaga tttgagcttt caaggataag gcttcttcca
     1681 cggtgctgac gagtcgaatc tctttggcat tacgcgagaa agcataagca gcggtggaga
     1741 aagctcgtaa aacgtcaatc acaaggacga tgccagtggc ttggtgacag tcgtcgagag
     1801 tatagcgccg gaatttcatc cgctaaacct tttgagaaga acaccgatca caacagccat
     1861 caaagaaaga aaaaatagaa aagtattaat ccttcccaac gctttcatct gaggtggcaa
     1921 gatattagca attccgaaaa agagcatggt tgccccaact gcaaagacct cccaagtatg
     1981 tgggtcgttg aaaatagcaa tacgattggg gcgaaggtca gtatagggca gtatgtttag
     2041 cgaaaccaaa attgttaata gaccacctaa aacaggcaac ccaccaagga ataatttccc
     2101 aatgaaacta ttggagaatc cgttttctga ggcaggttta ttttccataa tcaaaaacga
     2161 cctgtattgt ttcatgcgaa ttttcatcca acaatcgata ggcatttgcc gcctcgttca
     2221 gtgaaaatcg atgggtaatc cacttctgtg gttggatgcg ctccaaggct tgccatgcca
     2281 cctcgaagcg acgagacttg tcccagcggc cgcttagcgt gggcgaaatg ctgctaactt
     2341 gtgaagaaat cagttggatg cgtgagcgat gaaatgaacc gccaaggtcg atttctgctc
     2401 gtttttgccc ataccatgaa ccaattacga tccgtccgct gaatgaggtg tgttcaatgg
     2461 ctgtattcaa agcagaagga gagccagtga gttcaaagac gaggtcaaaa ctggaagatt
     2521 gaaggtcgct gggtgaaagt ttattgacct ttgacatttg accttcgatt tctttcattg
     2581 ctttccgtcg taattcaatt gcatctacaa cggtcaaatt ctcaagcgga aactcatgaa
     2641 gcagagaagc cgttagcaga ccaacgatcc cttgacccaa caccagcacg cgttcgccta
     2701 agattggagc gccatcttgc acaaggttga cggctgtttc catgtttgga aggaagcagg
     2761 cattttcagg cttaagcgaa tttggaatgg ggaacaggtt ttcaggttta cttaaaaaat
     2821 gagacgtgtg tggctgaaac gaaaacacca ggcgatcttg ccattctttg ccaaccacct
     2881 tgccaatctc gcgcactgtc cccacacaag cataaccata cgccagcggg tacttcaact
     2941 cgctgctaac cgcatcatgc gagtctttca actgtggaaa ttgatcacgg tagaccagca
     3001 tctctgtccc tgcgctaatg gcagagcaaa tggtttcgac taaaacttca tcctctttca
     3061 agagagggag gttagactct cgaacttcca cttgccgggg ggctgtaaaa aataaagttt
     3121 tagcaggaaa catggcgaaa gtttaacata aaaaggtgac agtcttttca gactgtcacc
     3181 ttttgttttc tactgctttg gtggtgctcc acgagttgga tatttgggga cgtcccgccg
     3241 gaattcatag tttttgataa tggcttcgat caaacctttt tgatctgggg taatttcttt
     3301 gaactcaaaa ccaatattga aatattgcgg gcttaggtct tgctcgcacc atgccactcg
     3361 ggttaataga ctcatacgaa ttgccttgat cccaggcaac tcaggcagtt caatggcgag
     3421 tgtgatttct gtatcgggct tcatggcttt ttcactgata atcattgccc ctgaaatggt
     3481 taaatcaccc aggtagccaa gcaaattccc accgtatagg tcgtacacct gtgtataagc
     3541 aactaaattt tttcgctctt ctttacgtcg ttcctgcata aaacctcatt ttgcacaagt
     3601 gtaaaggaag gggtgggcaa gttccattgc tgttttgtgg taaaaatatc gcaccatgcg
     3661 ctggatttat ctttcccctc atcttgatga tgccgccctc tccgctggtg gctggatcta
     3721 cgaccaaact caggcaggca acaaagttga gatatggacg tttctgtgtg gatacccgcc
     3781 cagcaaagaa ctttctatgt ttgcacgcat tctgcatatg cagtggggta tgcctacggc
     3841 acgtcaggtg attcgcatgc gccgtgcaga ggataaagcc gccgctacta ttcttggcgc
     3901 caaggtgcgg cacttcaact ttttagattg tatctaccgc cgcgatgtgg aggggaattg
     3961 gctctatacc gatgtctttg tggatccgca tcccagcgag gcaaatctcc ccgccgaaat
     4021 tgcttctgcc atttccgccc gcctcaaacc agacgataaa ctgatctgcc aatttggcgt
     4081 tggcaaacac gtggaccatg tcaccgtgcg ccgtgcagca gagttgcttg ggcgtccgct
     4141 tcattatgtg acggatattc catactattt caatacgccg gaacatttag ccccgaacac
     4201 agttgggatg aagaaaacgg tcgaacgggt gagcagagac gggcttgcgt cttggaaaga
     4261 ggcgattttg gcatacaaat cccagatctc gtcactattc aaaagtcaac gggcgatgcg
     4321 ggctcaaatc aaccagtact gctccgaatt taagggtatg cctttttgga cgacgaatta
     4381 gacaaaaaag gtaatacttt tgggggcttg aaacccacta taaatcatga tatagtatgc
     4441 gcgttttccg ggaagtacca ccggaaattt ttaatcattg catctcggta ggggatgcaa
     4501 aattcattta gcaggacaaa aaaatgccaa ccagtttcct taccaaagag ggttatgaca
     4561 agcttcaaga ggagcttgac cacctgcgca ctgttaaacg ccaggaagtt gccgagcgcc
     4621 tgcacgaagc catggaaggt ggcgagctga tcgaaaatgc tgaatacgaa gccgctaaga
     4681 atgaacaggc ttttgtcgaa ggacgcattc aagagctaga ccttttgctt gccacagctc
     4741 agatcattga agataatggc aaaggcaaaa agcacgatgc cattcaagtc ggctcaaaag
     4801 taaccatcaa agaaggcaat tacgaagccg aaacattcac cattgttggt gcagcagaag
     4861 ccaacccacg tgaaggcaag atctcaaatg agtctcccat tgggaaggct attttgggtc
     4921 acaaagtggg cgacaccgtg aaagtggaaa cccccggcgg tacgtacaat gttaagatcc
     4981 tcaaggtcgc ctaaatagga cgcgcctcgt tgccccgcgt cctatttttg ggatgcgggg
     5041 ctttttt
//
LOCUS       DAOQNJ010000129         5026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10096_length_5026_cov_10.099175, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000129 DAOQNJ010000000
VERSION     DAOQNJ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10096_length_5026_cov_10.099175"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1704
                     /locus_tag="PLG52_05145"
     CDS             154..1704
                     /locus_tag="PLG52_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB40862.1"
                     /translation="MEITYFEFVKHLIRFNRNVYLDHNATTPVSEHVRNKMDQALKYH
                     YGNPSSFYGSGRKSAGLVEEARGHVANAIHAEPYEVIFTACATESNNAVLKSVAAHFY
                     PQKKKIISTPIEHPSVLNTLAYLETQGVVVEFCPVDNKGRIQLTHLEKMVDNDTFLVC
                     CILANNETGVIQDIQAVTKIAKSHGALVLTDCVQALGKIPIDVHTWDVDYASFSAHKL
                     YGPKGVGALYVKQGSPFTPLLHGGHQEGGLRAGTESTHNIVGFGAACQQVDKLLAHVE
                     EVRALKHDLIQRLKEIKPDCVINSPEVDSECLPNTLSIRFPGVENSGLMGMLDYRGIA
                     VSAGSACSTGEDTPSHVLKAIGLSDQAARETIRISLGHDTSARDIQYTANVIRDYLEG
                     RISFVNMLAPAQLNETILFSDDTFILDVRPSDDRAKFKGIPNAHEVNPLFVENYIKQL
                     PRDKQIIVYCPGGGLSVMISYFLKAKGFKRITNLRGGLDAWRKRRADLYGKYAGQNVT
                     VLEPDAKR"
     gene            1835..2311
                     /locus_tag="PLG52_05150"
     CDS             1835..2311
                     /locus_tag="PLG52_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40863.1"
                     /translation="MNKGPFIVLRVLGILLILGLIAGAGAFGYKAGLAQGIQQAPEVA
                     AAFEKAAENGQVAMPGAPMMYGYGYPHMYGFGHPHFFNPFGAICFSFLFLFLFFGAMK
                     MLFFRRMRHGWGHHGHHGPWRKDWEGNVPPFFNEWHKRAHGESTEGETPADSEKKE"
     gene            2412..>2602
                     /locus_tag="PLG52_05155"
     CDS             2412..>2602
                     /locus_tag="PLG52_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB40864.1"
                     /translation="MPELILVVDDEPKIVRIARDYLEKNGFRVITAADGHAALATARR
                     EKPDLIVLDLMLPLMDGREV"
     assembly_gap    2603..2702
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2703..2960
                     /locus_tag="PLG52_05160"
     CDS             <2703..2960
                     /locus_tag="PLG52_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNB40865.1"
                     /translation="YSVTFNDEPVKLTPNEFKLLQLLSARPGQTLTREQLLEDMHGIA
                     SSFDRSVDSHIKNLRKKLETASGESMIETVYGIGYRFIEIK"
     gene            2965..4032
                     /locus_tag="PLG52_05165"
     CDS             2965..4032
                     /locus_tag="PLG52_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB40866.1"
                     /translation="MHMRRPHFPQRHPSNIERRRRRFFPFAFFFFGIFSLFFGGVAAI
                     LYWAFTEYSGFRNIWLLVCAAPIALLILGVFTIFTMYMRFGKPLGDIFNAIDSVAEGN
                     LSVRVDESKSDMFSELIKRFNKMVEELERAEQQRRNLTADIAHELRTPLHIIQGNLEG
                     VIDGVYQPSPEHINNTLDETKLLARLVEDLQTLSLAETGQLPLHPTRFLLADLTADLI
                     TSFSAQAKTQGIELTAHNADGEKTIEADYDRLNQVLSNLISNALRHTPADGKISIETE
                     SITGGERSVRITIQDTGSGIASEDLPFIFDRFWRADKSRTNRSNSGLGLAITKQLVLA
                     HKGDIRVESEIGKGTTFIIEI"
     gene            complement(4078..4764)
                     /locus_tag="PLG52_05170"
     CDS             complement(4078..4764)
                     /locus_tag="PLG52_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNB40867.1"
                     /translation="MEQMPSNTPVMESNPGPAGWVQTWIKAVTKPNEQTFIDISESPD
                     AKIQTALIWAAIAGLISGVVIGMSFALQQLIQGGNISDKVVTIAVAICGFPLAMAIIN
                     PIALSFSTALFQWVAKLFGGVGTFEKLIYPISAITVPMTIVSSVFSFLSSIPLVGACL
                     SIFSIAISIYATVLNIMAVKAVNRFDWGQAIASVLIPAFVIGIFCGCIVFIGMMAFMP
                     VISNAFGSFN"
BASE COUNT         1348 a         1203 c         1123 g         1252 t
ORIGIN      
        1 gcaggcagcc gacgccatga agcatttggg gacggggcat gcacgcggca agatcgtgct
       61 caaggtgaaa taagagggca tccattacca actattactt cggaacaacg tatactgaga
      121 gtaaccgtat catagcttta tcccggagtc tcaatggaaa tcacatactt tgaattcgta
      181 aaacacctca tccgcttcaa ccgcaacgtt tatcttgacc ataatgcaac gacaccggtc
      241 agcgaacacg tacgcaataa aatggatcag gcactgaaat atcattatgg taacccttcg
      301 tcattttatg gcagtggaag aaaatcagct gggttagtag aagaagcccg agggcatgtg
      361 gcaaatgcca ttcatgccga gccgtatgag gtgattttta cagcgtgcgc tactgaatca
      421 aataatgcgg tactgaaatc tgtcgcagcg cacttttacc ctcaaaagaa aaagatcatt
      481 tcaacaccga ttgaacaccc ttctgtgttg aatacattgg catatttaga aacacagggt
      541 gtggttgtcg aattttgccc agtggataac aaagggcgca tccaactcac tcacctcgaa
      601 aaaatggttg ataacgacac ctttttagtt tgctgtattc tagccaacaa tgaaaccggc
      661 gtgattcaag atattcaggc ggttacaaaa attgctaaat cacacggagc gctggtgttg
      721 acagactgcg tgcaagcctt gggaaagatc ccaattgacg tccacacttg ggatgtagat
      781 tacgcctctt tttcggctca caagctgtac ggtccaaaag gagttggagc gttatacgtc
      841 aagcagggaa gcccatttac accactgctg catggcgggc atcaagaagg cgggctacgg
      901 gcagggacag aaagcaccca caacatcgtc ggctttggcg ccgcctgtca acaagtggac
      961 aaacttctag cacatgttga agaagtccgg gcgttgaaac atgatttgat tcaacgtctc
     1021 aaagaaatta agccagattg cgttatcaat tcgcccgagg tggattcgga atgtctaccg
     1081 aacacactca gcatccgttt ccccggtgtg gaaaattccg ggctcatggg catgttggat
     1141 tatcgtggaa tcgccgtctc tgctggctcg gcgtgcagta ctggtgaaga tacgccatcg
     1201 catgttttga aagcaatcgg tttgtctgac caagccgctc gcgaaacgat tcgcatcagt
     1261 ctcgggcacg atacttcggc gcgagatatt caatatacag caaacgtaat tcgagattat
     1321 ctcgaaggtc ggatttcgtt cgtgaatatg ctcgctcctg cgcagctgaa cgagacaatc
     1381 ctttttagcg atgatacgtt tattctcgat gtgcgtccct ctgatgaccg ggcaaagttc
     1441 aaagggatac ccaatgcaca tgaggtcaac ccgctgttcg ttgaaaacta tataaagcaa
     1501 ctcccccgcg ataagcagat tatcgtctat tgcccgggcg gaggtctttc ggtcatgatc
     1561 tcttatttct tgaaagcaaa agggtttaaa cgcatcacca atctgcgcgg cgggcttgat
     1621 gcgtggcgca aacgacgggc tgacttgtat ggaaaatatg cagggcaaaa cgtgacagtt
     1681 cttgagccag atgctaaacg ttaagcaaga ggcgctaata attttcctac attccctcca
     1741 caatccctca cctgtccatc cataatccct ccacaatggg ttgatacatt tagacgcgta
     1801 aggacaaatc aaacaattac cattggaggt aaaaatgaat aaaggtccgt ttattgtttt
     1861 acgcgttctc ggtattctgc tcatcctcgg gctgatcgca ggcgcagggg cgtttgggta
     1921 taaggctggt ttggcacaag ggattcaaca agctcctgaa gttgccgccg cctttgagaa
     1981 agccgccgag aatggtcagg ttgctatgcc cggcgcaccc atgatgtacg gatatggcta
     2041 cccgcatatg tacggttttg gtcacccgca cttcttcaat ccattcggcg cgatctgctt
     2101 ctctttcttg ttcctcttcc tgttcttcgg ggcaatgaag atgctcttct tccgccgtat
     2161 gcgacatggc tgggggcatc acggacatca cggtccttgg cgcaaggatt gggaaggcaa
     2221 cgtcccccca ttcttcaatg aatggcacaa gcgcgctcac ggcgaaagca cagaaggcga
     2281 aacacctgcc gactctgaga agaaagagta acgtgtgaaa taaagaagaa agaggaagaa
     2341 acgcctcttt cttcttcccc tttcttaagc cctcactatc catccttcct cattcccttt
     2401 atactttccc catgcctgaa ctgatcctcg ttgttgatga tgaacccaag atcgtgcgta
     2461 tcgcgcgcga ctatctggaa aaaaatggct ttcgcgtgat tacggctgcc gacggtcacg
     2521 ccgcgctggc aacggctcgc cgcgaaaagc ctgatctgat cgttttggac ttgatgctcc
     2581 ctctcatgga cgggcgtgaa gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nntactcggt cacattcaac gacgagcccg tcaaacttac gcccaatgag ttcaagcttt
     2761 tacagttgct ctccgcccgc ccgggtcaga ccctgacccg tgaacaatta ctcgaagata
     2821 tgcacggcat cgcatccagt tttgaccgta gtgtagactc gcacatcaaa aatctacgca
     2881 agaaactcga aactgcttct ggagagagta tgatcgagac cgtatatgga attgggtatc
     2941 gtttcattga aataaaataa agccatgcat atgcgtcgtc ctcactttcc gcaaaggcat
     3001 ccatccaata tcgagcgccg tcgaaggcgt ttttttccat ttgcattttt cttctttggt
     3061 atcttttccc tgttctttgg cggtgtagcg gcgattttat attgggcatt cacagaatat
     3121 tctggctttc gcaatatttg gctgttggtt tgtgcagcgc caattgcctt actgatcctc
     3181 ggcgtattta ccatcttcac catgtatatg cgtttcggca aaccgcttgg tgatatcttc
     3241 aatgccattg attctgttgc agaggggaat ctctcagtac gtgttgatga gagcaagtct
     3301 gacatgttca gtgagttgat caaacgcttc aataaaatgg tcgaagaact ggaacgcgct
     3361 gaacagcaac gtcgcaatct cactgccgat attgcgcatg aattgcgcac gcctttgcac
     3421 attattcaag gcaacctcga aggcgtcatt gatggtgtgt atcaacccag cccggaacac
     3481 atcaacaaca ccttggatga gaccaaacta cttgcccgct tggtagagga tctacagacg
     3541 ctctcgctcg ccgaaacagg acaacttccg cttcacccta cccgcttcct gcttgccgat
     3601 ctgacagcgg atctcatcac cagtttttca gcacaagcga aaactcaagg catcgaattg
     3661 actgctcata acgcagatgg tgagaaaacg attgaagccg attatgacag gcttaatcag
     3721 gtattatcca atctcatttc caatgcatta agacacacgc ctgcagatgg aaaaatttca
     3781 atcgaaacag aatccataac cggcggagaa agaagcgtgc gcatcaccat ccaagataca
     3841 ggttctggga ttgcatccga agacctgccc tttatcttcg atcgcttttg gcgggcagat
     3901 aaatcacgaa ccaatcgctc gaatagcggc ttgggtcttg cgattacaaa acagcttgtg
     3961 ctggcacaca aaggtgacat tcgtgtggaa agtgaaattg gaaaaggcac gacatttatc
     4021 atcgagatat aaaaaagtcc tcccaaatgg gaggactttt ttatcgtttg tgtatcttta
     4081 attgaagctt ccgaaagcat tcgaaatgac aggcataaac gccatcatac cgatgaacac
     4141 gatgcagcca cagaagatgc cgattacaaa agccggaatc aataccgaag caattgcctg
     4201 cccccagtca aaacgattta ctgctttaac cgccataata ttcaagacag tcgcataaat
     4261 acttatagca atactaaaaa tgcttaaaca cgcccctaca agaggaatac tgcttaaaaa
     4321 gctaaacacg cttgaaacaa tcgtcatcgg cacggtaatg gctgaaattg gataaataag
     4381 tttttcgaac gtgcctaccc cgccaaagag ttttgcaacc cactggaaca atgctgtgga
     4441 aaaagatagg gcaattgggt taataattgc catggcgagc ggaaagccac aaattgcaac
     4501 ggcaatagta actaccttat cagaaatatt gccaccctga atcagttgtt gtagcgcaaa
     4561 actcatccct atcacaaccc cgctaataag ccctgcgatg gctgcccaaa taagcgctgt
     4621 ttgaattttt gcatctgggc tttcagagat atcaataaac gtttgttcat ttggttttgt
     4681 aactgctttg atccatgttt gaacccagcc tgcgggtccg gggttggatt ccatgacagg
     4741 tgtgtttgaa ggcatttgtt ccatttgaat tctccttttt ctttccaaag tgatgacgta
     4801 tccattttaa gcgatgatat tccgcttgac cgtactgtat ttcaacggca attttcagaa
     4861 caaatatcct aatttcgata acaagcggta gaattgccgc aatggaagtt tcagagaagc
     4921 tacaaggcat tcttgccacc ctgcccacca aaccgggctg ttatctatat cgcaacgccg
     4981 aagggacaat catttacgtc ggcaaggcga tcaatttgaa gaaccg
//
LOCUS       DAOQNJ010000130         5022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10113_length_5022_cov_7.373264, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000130 DAOQNJ010000000
VERSION     DAOQNJ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10113_length_5022_cov_7.373264"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PLG52_05175"
     CDS             <1..570
                     /locus_tag="PLG52_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB40868.1"
                     /translation="IRSLTDDMIQITTLATDLYSLNPEAVDLNSIIDNAMSYTSSQIR
                     EKNISIHLEVPKAVTSIHADREALQQIVIHLLQNAGAATPFEGTIRLKVQTQNEDGAE
                     YILIQVADQGGGIASEDIPRVFTRLYRADNVLIQGVGDTGVGLSIAKTLTEAQHGRIW
                     VESKVGEGSTFSVLLPISGDKLADKQKRK"
     gene            574..1383
                     /locus_tag="PLG52_05180"
     CDS             574..1383
                     /locus_tag="PLG52_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB40869.1"
                     /translation="MRGVRDFGNALVAAILSIGLTLGALSISLVGFIPEEVATPSPTL
                     IVSPIPVTATNTLPPTATLLVTVNIPTSSPTNTIVPPTSCQPPVGWVGVVVQVGDTLD
                     SLAARYGTYKDILKNKNCLFTDSLIAGTLLYVPGGISTSTVVACNQGAVGWIKNYMVR
                     PGDTFYNIGTRYGVNANLIKLVNCRTSDIIYAGEILYVPNVPTRTPTFTPLPGITYTA
                     APLLTEPLTLTVLPFTATYPPTQTSTATTAPTASPIPTQTASLTAIPTPTP"
     gene            1410..>2239
                     /locus_tag="PLG52_05185"
     CDS             1410..>2239
                     /locus_tag="PLG52_05185"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNB40870.1"
                     /translation="MKSSNLFVWALCLAVFISACGAAAPTQAPQPFVLITAAPNASPT
                     PTPFQPSLASPTAPFNASGSNPAPQPILATETTFPTATPQASPTATAPIEQLFPTQAA
                     PPPAEALVNPTALPPLTTENDTINFLLVGSDRRPTGTSHRTDTLLIAVVWYKEGQVSL
                     ISIPRDTWLYIPTVGMQRINTAYQSGEGVYPGGGMGLLKDTILQNFGIRIDHTAMVEF
                     DGFRRIVDTLGGIDVPISCPYTDWRLISPDLDENDENNWWLYTVGPGQIHMDGDLALW
                     "
     assembly_gap    2240..2339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2340..3055
                     /locus_tag="PLG52_05190"
     CDS             <2340..3055
                     /locus_tag="PLG52_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNB40871.1"
                     /translation="YTVGPGQIHMDGDLALWYARSRSKSSDFDRGRRQQEVIRTIFQK
                     ALQTGTFTKIPQLYNDFSSTVVTDMSLGDMLSLAPYAVNFTNANIRSYYIRPPYVLPW
                     TTPGGAAVLLPQDSLSSMLVEATTLSTYAATRETVTVEVQNGSYFDTMETLAASRLNY
                     AGYETITSPADNRNYGSSVLVDYTVAQDPSQRQTIIDVLGTYSANVLSMPDPNSKVQY
                     RVIIGADYEACFRPEDLAH"
     gene            3098..3171
                     /locus_tag="PLG52_05195"
     tRNA            3098..3171
                     /locus_tag="PLG52_05195"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3132..3134,aa:Val,seq:cac)
     gene            3290..3928
                     /locus_tag="PLG52_05200"
     CDS             3290..3928
                     /locus_tag="PLG52_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB40872.1"
                     /translation="MSSRTNETWLSDLRSSGPVHEEALSDLRAIIQKGLPYALSRWLS
                     SDDPLFPPLVEEVTQETLLRVLDQLDTFEGRSLFTTWVHKIAIRIALTELRRKRWRDA
                     SLDELTENEDAPPPPGLLADPHASPETSAERKDMVSRVRRILEEELTPKQREALVLLG
                     LQDMPMEDAAKRMKTNRNALYKLLHDARLRLKKRLALEELTPQDVLLAFEQK"
     gene            3996..4292
                     /locus_tag="PLG52_05205"
     CDS             3996..4292
                     /locus_tag="PLG52_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40873.1"
                     /translation="MNNEHLPTPQNKPSQPEITDEVALRFLKILEQVREEDLSCSDMY
                     ARLDEFVEAEIKDGKDAAILTPLIHEHLDMCSGCCDEYEALLNVVENTSNTQDE"
     gene            complement(4323..4829)
                     /locus_tag="PLG52_05210"
     CDS             complement(4323..4829)
                     /locus_tag="PLG52_05210"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNB40874.1"
                     /translation="MSELELFRAEKNEFFGSHPQSPLTREQKKDFHGLNYFPENETLR
                     LEVKVEEFSVKDELVMQTTTGGVQHYERFGKFSFDVESQTVELTVYRSEHGFFLPFVD
                     ALAGSETYPAGRYLEPDPLPGGRFFIDFNLAYNPYCAYNEMWSCPITPAENRLKVPIR
                     AGEKLFHE"
BASE COUNT         1304 a         1405 c         1009 g         1204 t
ORIGIN      
        1 atccgcagcc tgacagacga tatgatccaa atcacaacgc tggctaccga cctgtattcg
       61 ctcaatcctg aagctgtaga cttgaactca atcatcgaca atgccatgtc gtacaccagc
      121 tctcagattc gtgagaaaaa tatttccatt catctggaag ttccaaaggc agtcacatcc
      181 atccacgcgg acagggaagc cctacaacaa attgtcattc acttgttgca aaacgccgga
      241 gcggcaactc ccttcgaagg gacgatccgc ctcaaagtcc agacccagaa cgaagacggc
      301 gctgagtaca ttttgattca agtcgcagat caaggtggcg gaattgcctc cgaagatatt
      361 ccgcgcgttt ttacccgtct ctatcgagct gataatgttc tcattcaagg ggttggtgat
      421 accggcgttg gtctttccat tgccaaaacc ctcaccgaag cccagcatgg tcgtatttgg
      481 gttgaaagca aagtgggaga aggctccacg tttagtgtgc ttttgccaat ctctggcgat
      541 aaacttgccg acaagcaaaa aaggaaataa acaatgcgag gcgtgcgcga ttttggaaac
      601 gcgcttgttg cggcaattct ctcaattggg ttaacactgg gagcgttgtc gatttctctt
      661 gttggcttta tccctgaaga agtcgccact ccatccccaa cactgattgt ttctcctatt
      721 ccggtaactg ctacaaacac cttgcctcca acggctactt tgttagtcac cgttaatatt
      781 cctacatctt caccaaccaa taccattgtt cctccaacct cctgtcaacc acctgtgggc
      841 tgggtgggag ttgttgttca agtaggcgac acgttagata gcctcgcagc aaggtatggc
      901 acgtataaag acattctcaa aaacaaaaac tgccttttca cagacagtct tattgccggc
      961 acgcttctat atgtgccggg tggcatttcc accagtacag tagttgcttg taaccaaggc
     1021 gccgttggtt ggattaaaaa ctatatggtt cgcccaggcg ataccttcta taacatcggc
     1081 actcgctatg gcgtcaacgc caacttaatc aaacttgtca actgccgaac tagcgacatt
     1141 atttatgcgg gtgaaattct ttatgtacca aacgtaccca cacgcacacc aacctttaca
     1201 cctttgcctg gtattaccta caccgcggct ccattactca ccgaaccatt aaccctaacg
     1261 gttctgcctt tcacggcaac atacccgcca acccaaacat ccactgccac caccgcacct
     1321 acggcttcgc ccatcccaac ccaaaccgcc agcttaaccg ctatccccac cccaacgccc
     1381 taagcaggcg cactttccat aacacatcga tgaaatcatc caaccttttt gtatgggcgc
     1441 tttgccttgc cgtttttatt tcagcttgtg gtgcagcggc tccgacccaa gcgcctcagc
     1501 cttttgtttt aatcactgcc gctccaaacg catcgcctac accaacaccc tttcaacctt
     1561 ccttagccag cccaactgca ccttttaatg caagcggatc aaaccccgcc ccacaaccga
     1621 ttcttgcaac tgaaactaca ttcccaactg ccacgcctca agcctcaccg acagcgacag
     1681 cccccattga acagctattc cccactcaag ccgctccgcc tcctgctgaa gccttggtca
     1741 accccacagc cttaccaccc ctcaccaccg aaaacgacac cattaatttc ctactcgtcg
     1801 gctcagaccg cagaccaacc ggaacctctc accgcacaga cacattactc attgctgtgg
     1861 tttggtacaa agaaggtcaa gtctccctca tctccattcc gcgcgatact tggctttata
     1921 ttccaaccgt aggcatgcaa cgcatcaaca ccgcttatca aagcggagaa ggcgtgtacc
     1981 ccggcggcgg gatgggactt ttgaaagata ccattctgca aaacttcggt atccgtatcg
     2041 accatacagc catggtggag tttgatggct ttcgccgcat cgtagatacc ctcggtggga
     2101 tcgatgtccc catatcctgc ccctatacgg attggcgtct catctccccc gatctggacg
     2161 aaaatgacga aaacaactgg tggctataca cagtcggacc aggtcaaatt catatggatg
     2221 gcgatctcgc cctttggtan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2341 atacacagtc ggaccaggtc aaattcatat ggatggcgat ctcgcccttt ggtatgcccg
     2401 ctcccgctcc aaatctagcg actttgaccg cggacgacgt caacaagaag taatccgtac
     2461 catcttccag aaagcccttc aaaccggtac attcacaaaa attccgcagc tctacaacga
     2521 tttctcaagc acagtcgtca ctgatatgag ccttggcgac atgctcagcc tcgcacccta
     2581 tgcagtgaat ttcaccaatg ccaatattcg cagctactac attcgcccac cctacgtcct
     2641 gccgtggaca acgcccggcg gagcagccgt ccttctgcca caagactcat taagctctat
     2701 gctggtcgaa gcgacaactc tttcgactta tgccgcaact cgcgaaaccg tcacagtaga
     2761 ggtgcaaaac ggctcatact ttgacacgat ggagaccctc gccgcctcac gcttaaatta
     2821 tgctgggtat gaaaccatca ccagcccagc agacaaccga aactatggca gctccgttct
     2881 ggttgattac actgttgctc aagatcccag tcaacgccaa accatcatag acgtgttggg
     2941 cacctattca gccaatgttt tgtccatgcc agacccgaac agcaaggttc aataccgcgt
     3001 catcattggt gcagattatg aagcctgttt tagacccgaa gacctggcac attaggcttg
     3061 caaaagacga gaggctcggc tataatctcg ctccgttggg tgcgtagctc agttggttag
     3121 agcactacta tcacacagta ggggtccggg gttcgagtcc ctgcgcgccc acagaaaacc
     3181 gtctccttga ggcggttttt ttcttttccg aaacggattt ataaaaatac tatcagaatc
     3241 tctcagggat gctgaaaaac cccttgcagt atcttataat gactacgtta tgtcttctcg
     3301 cactaatgaa acttggttaa gtgatttacg cagttctggt cccgtccatg aagaggcgtt
     3361 aagcgacttg cgtgcaatca tccaaaaggg cttgccgtac gcactatcac gctggctatc
     3421 gtcagatgat cctctctttc ctcccctcgt cgaagaagta acccaggaaa ccctcctacg
     3481 cgtattagac caattggata cctttgaagg gcgcagcctt ttcaccacct gggtacacaa
     3541 aattgccatc cgtatcgcat tgaccgagct tcgccgcaag cgctggcgtg acgcgtctct
     3601 cgatgagctg acggaaaatg aagacgctcc tcctcctccc ggtctgcttg cagaccctca
     3661 tgcatcccct gaaacctctg ccgaacgcaa agatatggtc tctcgtgtac gccgcatctt
     3721 ggaagaagag ctgaccccaa aacagcgaga ggcgctcgtt ttgcttggct tgcaagacat
     3781 gcccatggaa gatgccgcaa aacgcatgaa aaccaaccgc aatgcattat acaaactgct
     3841 tcacgatgcc cgcctgcgcc tcaaaaaacg cctagcgctg gaagaactga ccccacaaga
     3901 tgttctactc gccttcgagc agaaataatt tcttatcaat tgggtataat tgacaaagta
     3961 agattaagtt tttattaatc gtctcaccct ttagaatgaa taacgaacat ctccctaccc
     4021 ctcaaaacaa gccttctcaa cctgagatta cggatgaagt tgccttgcga ttcttgaaga
     4081 tccttgagca agttcgtgaa gaggatctct cttgcagtga catgtatgcg cggctggatg
     4141 aatttgtgga agccgagatc aaagacggta aagacgccgc cattctcact ccactcatcc
     4201 atgagcattt agatatgtgt tccggctgtt gtgacgaata tgaagcttta ctgaatgttg
     4261 tagaaaatac aagcaatact caagatgaat aaaaaaaatg acgggctagc ccgtcatttt
     4321 ttttattcat ggaatagttt ctcaccagcc cgtatgggca ccttcaagcg attctcagcc
     4381 ggggtgattg ggcaggacca catttcattg taggcacagt atgggttgta tgccaagttg
     4441 aagtcaataa agaagcgccc acctggcaaa gggtctggct caaggtaacg cccggcgggg
     4501 tacgtctcac taccagccaa cgcatccacg aacggcagaa aaaagccatg ctcgctacga
     4561 taaacggtca actctacggt ttgactctcc acatcaaaag agaattttcc aaagcgttca
     4621 tagtgctgaa caccacctgt ggtggtttgc atcaccaact catccttaac tgaaaattct
     4681 tccactttca cttccagccg taaagtttca ttttctggaa aataattcaa cccatgaaag
     4741 tcttttttct gctcacgggt tagcgggctt tgcggatggc tgccaaaaaa ctcattcttt
     4801 tctgcacgaa aaagctccaa ttcactcatt gatacctcca aatcttattt tgcatagaca
     4861 tggaacaaat ccgaattctt acaaggacat cgcaaaaatc atgcgccctt gcaaattcca
     4921 tacctttatg ctatttttag catagtagag gaaacaaaat gaattcgaga caaaaaacgg
     4981 tcatgatcat cgaagacgaa gccgatgccg ctgaactttt tt
//
LOCUS       DAOQNJ010000131         4995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10179_length_4995_cov_10.582186, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000131 DAOQNJ010000000
VERSION     DAOQNJ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10179_length_4995_cov_10.582186"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLG52_05215"
     CDS             <1..363
                     /locus_tag="PLG52_05215"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNB40875.1"
                     /translation="ALARLYTDPATDSATQAAVAAVFTAVHQYGGVILGEHMGQFLII
                     LWMSMISGIMYKSKMFSKWVAILGWVASAIYLLAQTELLATAIPTFPVIDWAGLVGSL
                     LWLVWMIVMGVYLVKYKE"
     gene            365..1255
                     /locus_tag="PLG52_05220"
     CDS             365..1255
                     /locus_tag="PLG52_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNB40876.1"
                     /translation="MQFPEPFLSNIKHSFGEAGETFLASLPALIEEASQRWQLTDIQP
                     VSNLSYNFVAFAQRGRDKVMLKIGVPHRELISEMAALILFNGDGACQIIDCDEGKGFL
                     LIEQLQPGKMLADLEDDDERTRIAIEVMKKIWRPAPETGKFIQLEDWFAELENIRPAF
                     DGTTGPYPKELLEKVESFLPELFKDEHYLIHGDFHHFNILSSERGWLAIDPKGVIGPC
                     GYEVGPLMLNPWNNSMDSAQFEVRAKRRVDLLHEGLGWEREQLIQWSTAHALLSSWWD
                     YPNGDWQYSLRCANIFAGLK"
     gene            complement(1297..1446)
                     /locus_tag="PLG52_05225"
     CDS             complement(1297..1446)
                     /locus_tag="PLG52_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40877.1"
                     /translation="MKVSLPKTISGWCMWLFFLCFGLATFVPVLGTITPWLALAYAVL
                     SLIGM"
     gene            1559..3343
                     /locus_tag="PLG52_05230"
     CDS             1559..3343
                     /locus_tag="PLG52_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40878.1"
                     /translation="MTSLSVPAEFTLQRKNKYDQSTTLRWILSHVQPYWWIVAMIFIG
                     AIGNAVLAVVIPVLTGDAFNAMLAPKPDTSVLLPLAVTIGISQIIRGVLQLGRNFGAE
                     LMAQKMERDIRDELYLSLLGKSMTFHNLQPVGDTMARSTNDVREVNYMFSPGVNLVVG
                     SFMFILMPLFFGPRYHPALVLTPLLFTIFYFIALVRYLKTLSPITDDVRATFGQMNTH
                     LSESLDGVEIMKGAAQEEAEVDRFVTNARKVRDAFVQQGDLEARYIAMLLLGLAFAGG
                     LVHALFLLRQGLINVGAVIGYFGMLQLLGFPTFTSTFAYSQISLGFSSARRILELIQR
                     ETKLDQNASGMTSEIRGEIEFRNVSFHYPTPAEGGAGGDNVLENISFKVKPGQTVALV
                     GQTGAGKTTLVKLINRIYDVTDGQVLVDGVDVREWNLASLRSQISIIEQDIFLFSRTL
                     SDNIAFGKPGATKEEIIAASMAAQAHDFILDFDETYATVVGERGVTLSGGQRQRIALA
                     RAFLTEPHVLVLDDSTSAIDSATEDKIQRAISNAAKGRTTFIITHRLSQIRWADLIIV
                     LRKGKVVAMGTHDELMKTSEAYSRIFRE"
     gene            3350..>4995
                     /locus_tag="PLG52_05235"
     CDS             3350..>4995
                     /locus_tag="PLG52_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40879.1"
                     /translation="MGFFAGLNDEKYDRQYKDNELVRRMVNYFNTQKTRLVFASVVII
                     VIAVIGAALPVIVSRMVDLIKDQPSTSDILLVGAALVGVAFGNWGLNWLRRGLIVRAV
                     GDVVLDMRSRAFRAAAEHDLSFYDQFSSGRIVSRITSDTNDFGQLVVIVTDVVAQFIQ
                     AFLIAIVLFRTELRLSLLLIGFLPIIFGIAAGFRAMARRVTKKGMKAMADVNAAIKET
                     ISGISIAKNFRQEASIFKSFDESNIQSYDVNVQRGFVLSLVFPTLNALGGIFIGVLVY
                     VGGHSAAEGAISIGAWYLFIMSLDQFFFPVLNLTSFWAQIQGGLSAAERVFALIDADP
                     NVVQAEKQDVPRLKGEIHFDHMHFRYSDKEPVLNDFNLLIKPGETLALVGHTGAGKSS
                     IAKLIARFYEFQQGRLLIDGRDIRTFDLTQYRRQLGIVSQVPFLFSGTIADNIRYAAP
                     GAPEEEMLRLAKRIGDGEWLDALPNGLYTEVGERGNRLSMGQRQLVALMRVLVQNPAI
                     FILDEATASIDPFTEWQIQQALNLILKNSTSILIAHRLSTV"
BASE COUNT         1175 a         1318 c         1211 g         1291 t
ORIGIN      
        1 gccctcgccc gcctctacac ggacccggca acagattcag ctacccaagc cgcagtcgcc
       61 gctgtattca ctgccgtcca ccaatatggc ggagtcatcc tcggcgaaca tatgggacaa
      121 ttccttatca tcctgtggat gtctatgatc agcggcatta tgtacaaatc caagatgttc
      181 agcaaatggg ttgccattct gggatgggtt gcctctgcca tttacctcct cgcgcagacg
      241 gaacttctcg ccaccgctat tcccactttc cccgttatcg attgggcggg actggttggc
      301 agcttacttt ggctggtctg gatgatcgtg atgggagtat atttggtgaa atataaggaa
      361 tagtatgcaa ttccctgaac cctttctctc aaacatcaaa cattcttttg gcgaggctgg
      421 cgagacattt cttgccagcc tccctgcttt gatcgaagaa gcgtcccaac gctggcagtt
      481 gaccgacatt cagcctgtga gcaatctctc gtataacttc gtcgcctttg cccagcgtgg
      541 acgtgataaa gtgatgctca agatcggtgt gccacaccgc gaactaatca gcgaaatggc
      601 agcgctgata ttgttcaacg gtgatggagc ctgtcagatc attgattgtg atgaaggaaa
      661 gggattttta ttaattgaac aacttcagcc aggcaaaatg ctggcagact tggaagatga
      721 tgacgagcgg actcgcattg caatagaagt catgaaaaaa atctggcgcc ctgcacctga
      781 aaccggaaag tttattcagt tggaagattg gtttgcagag ttagaaaaca tccgccccgc
      841 tttcgatgga acaaccggac cataccctaa agaattgttg gagaaggttg aatcatttct
      901 accggaatta ttcaaagatg agcattattt gattcatggt gattttcatc atttcaacat
      961 tctctcttca gaacgtggtt ggctcgccat tgaccctaag ggtgtgattg gtccttgtgg
     1021 ctatgaggtc ggtccgctca tgctcaaccc gtggaataac tcaatggaca gtgcccagtt
     1081 cgaggtccgc gcaaagaggc gggtggactt gctccatgaa gggttaggct gggagcgcga
     1141 gcagcttatc caatggtcca cagcccatgc cctcttatcc tcctggtggg attatcccaa
     1201 tggcgattgg caatactcgt tacggtgtgc caatattttt gcagggctta aatgacacaa
     1261 cctccctttc gggaggttgt gtgtttatca attgagttac ataccaataa ggcttaagac
     1321 ggcgtaggca agagccagcc agggagtgat ggtgcctaat acgggcacga atgttgcgag
     1381 accaaagcac aagaagaaca gccacataca ccaaccagaa atcgttttgg gcaaagaaac
     1441 tttcatttca cactctccta taaattgttg gcgctggcag aattgccagc tacaaaataa
     1501 aacaccttac taaagtgagg gttgcggtgc tcttttttag ggatgttaca attactttat
     1561 gacttcccta agtgttccag cagaatttac tttacagaga aagaataagt acgatcaatc
     1621 cacaacattg cgatggattc tttctcatgt acagccttat tggtggatcg tagccatgat
     1681 cttcattggt gcaattggca acgccgtatt ggctgttgtc attccggtgt tgaccggtga
     1741 tgcattcaat gccatgcttg cccccaagcc agatacctct gtgcttctgc cgttggctgt
     1801 aacgatcggc atttcacaga tcatccgtgg tgtgctgcaa ttggggcgta actttggtgc
     1861 cgagttgatg gcacaaaaaa tggaacgaga catccgcgat gagttgtatc tctcgctgct
     1921 tggcaagagt atgaccttcc ataacttaca acctgtgggc gatacaatgg cacgctctac
     1981 gaacgatgtg cgcgaagtga attacatgtt tagccccggc gtgaatcttg tggtcggttc
     2041 gttcatgttc atcctcatgc cactgttctt tggtccgcgc tatcatccag cgttggtact
     2101 caccccgctg ctatttacca ttttctattt catcgcattg gttcgatatc tcaaaacgct
     2161 ttcgcctatt accgatgatg tgcgtgcaac gtttggacag atgaacacgc atctatctga
     2221 gtcacttgat ggggtcgaga tcatgaaagg tgcggcgcaa gaagaagccg aagtggaccg
     2281 tttcgtgacc aacgcccgca aagttcgtga tgcttttgtg cagcaaggtg accttgaagc
     2341 gcgttacatt gccatgctct tactcggtct ggctttcgca ggcgggcttg tccacgcatt
     2401 gtttttgctg cgccaagggc taatcaacgt tggagccgtg attggctatt ttggcatgct
     2461 gcaactcttg ggctttccca ctttcacctc aacctttgcc tactctcaaa tctctttggg
     2521 attttcatcc gctcgccgta tcttggaact cattcaacgc gagacaaaac ttgaccaaaa
     2581 cgcctcaggc atgacctcgg agattcgcgg cgaaattgag tttcgcaacg tctcgttcca
     2641 ctaccctaca cctgctgaag gcggtgccgg tggagataat gtacttgaga atatttcatt
     2701 caaagtgaaa cccggtcaga ccgtggcttt ggttgggcag accggtgctg gcaagaccac
     2761 cctcgtcaag ctcatcaacc gcatctatga tgtgacagat gggcaagtgc ttgttgatgg
     2821 tgtagacgtg cgcgaatgga atctggcatc gctgcgttct cagatatcca tcattgaaca
     2881 agatatcttc ctcttctcgc gcacccttag cgataatatc gcctttggca aacccggtgc
     2941 gaccaaagaa gaaatcattg ctgcatccat ggcagctcaa gcgcacgact tcattttgga
     3001 ctttgacgaa acctatgcaa ccgtggtcgg cgaacgcggt gtgactctct ccggtgggca
     3061 acgtcaacgc atcgcgctgg ctcgtgcctt cctcaccgag ccgcacgtgc tcgtcctcga
     3121 cgactcaacg tccgcgattg actcagcgac ggaagacaaa attcaacgcg ccatctccaa
     3181 cgccgctaaa ggacgcacca ccttcatcat cacccaccgt ctctcccaaa tccgctgggc
     3241 agacctcatc atcgtgctcc gcaaagggaa agtcgttgcc atgggcaccc atgacgaatt
     3301 gatgaaaaca tcggaagcat attcaaggat atttagggaa taacaggtta tgggtttctt
     3361 tgcaggatta aacgacgaaa aatacgaccg ccagtacaaa gacaatgaac ttgtccggcg
     3421 catggtcaac tatttcaata cacaaaaaac acggctggta tttgcctcgg tagtcatcat
     3481 cgtcatcgcg gtcatcggcg cggcattgcc agtcatcgtc agccgcatgg tggatttgat
     3541 caaagatcag cccagcacat cagatattct tttggttggc gctgcccttg tcggtgtggc
     3601 atttggcaac tggggactga actggctcag gcgtggtctg atcgtgcgcg ccgtcggtga
     3661 tgtggttctg gacatgcgct cccgcgcctt ccgggctgca gctgaacacg acctttcctt
     3721 ttacgaccag ttctcttccg ggcgtatcgt ctcgcgcatc acatcagaca cgaatgactt
     3781 cggtcaactc gtggtgatcg tcactgatgt agtggcacag ttcattcaag ccttcctcat
     3841 cgctatcgtg ctcttccgca ctgagttgcg gctttctctt ttgctcatcg gtttcttgcc
     3901 gatcatcttt ggaattgcag ctggcttccg cgccatggca aggcgtgtca ctaaaaaagg
     3961 catgaaagcc atggcagatg tcaacgccgc catcaaagaa accatcagcg gcatttcgat
     4021 tgcaaaaaac ttccgtcaag aagccagcat cttcaagtcc ttcgatgaat caaacatcca
     4081 atcgtatgat gtgaacgttc aacgcggctt tgtgctttca ctggtcttcc ctaccctcaa
     4141 tgcactcggc ggcatcttca ttggtgtgtt ggtgtacgtg ggcggacata gcgccgcaga
     4201 aggcgccatc agcattggcg catggtatct cttcatcatg agcctcgacc agttcttctt
     4261 ccctgtgctc aacctcactt cattctgggc acaaattcaa ggcgggctct ctgcagccga
     4321 acgcgtcttc gcattgatcg acgcagaccc aaatgtagtt caagccgaaa aacaggatgt
     4381 accgcgactc aagggtgaga ttcatttcga ccacatgcac ttccgctatt cggataaaga
     4441 accggttctc aacgacttca acctgctcat caaacccgga gagactcttg cccttgtcgg
     4501 tcataccggc gcaggcaagt cttccatcgc caaactcatt gcccgattct acgaattcca
     4561 acagggacgt ctcctcatcg acggacgcga tatccgcacc tttgacttga ctcagtatcg
     4621 ccgtcagttg ggcatcgttt cgcaagtgcc cttccttttt tctgggacga ttgcagataa
     4681 cattcgctac gctgcccccg gcgcacccga agaagaaatg ttgcgccttg ccaagcgcat
     4741 tggcgacggg gaatggttgg atgctctccc gaacggactg tataccgaag ttggtgaacg
     4801 cggcaatcgc ctttcgatgg ggcaacgtca actcgtggcg ttgatgcgcg tgctcgtgca
     4861 gaaccccgcc atcttcatcc tcgatgaagc gactgccagt attgatccgt tcacagagtg
     4921 gcagatccaa caggcgttga atcttattct caaaaattca accagtatcc tcatcgcaca
     4981 tcgtctctcc accgt
//
LOCUS       DAOQNJ010000132         4969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10249_length_4969_cov_8.465201, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000132 DAOQNJ010000000
VERSION     DAOQNJ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10249_length_4969_cov_8.465201"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLG52_05240"
     CDS             <1..699
                     /locus_tag="PLG52_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB40880.1"
                     /translation="VFRAGDQISEVLNIHQDFGKEAGRARAVELLKQVGIPEPNRRAE
                     AFPHELSGGMSQRVMIAMALACVPDLLIADEPTTALDVTIQAQILDLMRDLRNELGSA
                     LMLITHDLGVIAEMANRVVVMYAGEIVEESPVASLFDKPHHPYTQGLIGSIPVLGQRR
                     DRLDVIPGNVPNLVNLPQGCRFAPRCRARTEHNLSICTEKHPGLIQVADGHKVRCWLY
                     EDADGHTAPLKSGK"
     gene            714..1703
                     /locus_tag="PLG52_05245"
     CDS             714..1703
                     /locus_tag="PLG52_05245"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNB40881.1"
                     /translation="MNKINKQEQPELLVVDNLVKYFPVRTGLFQRVTAWVQAVDKVSF
                     AVKKGETLGMVGESGCGKTTVGRTLLRLIEPTSGNVTFEGKDVLKLNQRELKPLRRDM
                     QIIFQDPYASLNPRMPIGESVMEGLQIHNIGHPKERWEIAVNMLKKVGLEEYHARRYP
                     HEFSGGQRQRIGIARALALNPKFIVCDEPVSALDVSIQSQVLNILKDLQEEFGLTYLF
                     IAHNLSVVEHISDRVAVMYLGKMVELTDRDSLYREPLHPYTKALLSAIPVGHPNEKRE
                     RTILKGDVPSPLNPPKGCRFHTRCPIAVSQCSQEEPTFKEVRPGHWVACLLAQ"
     gene            1792..3717
                     /locus_tag="PLG52_05250"
     CDS             1792..3717
                     /locus_tag="PLG52_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB40882.1"
                     /translation="MKKNVIPFLLVIVLIVIPACLSAPAQTETPAPTESFIEITETPP
                     PSLGPSLTVCLGQEPNSLYPFGDLNDAARNVLSAIYDGPIDNVGYEYQPVILTKLPSI
                     ENGDAQIVPIKINNGDSVVGADGNIIQFSKGSIVRPAGCRNADCAITYDGTSSIEMDQ
                     MIVTFRLRPDLTWSDGTPLTADDSIYSFELQKESKVNTYLTERTQTYEAADEQTLQWF
                     SIPGYLDPTYFLNFWQPAPKHAWSEFTPDQLPTVDVSSREPLGWGPYTVKEWLPGEGL
                     TLEKNPYYYRSRDGYPKLDSIQFRFIPDPDLALSELIAGRCDLIDPTINLEDKVGLLQ
                     EMQTSGQAKFFATTGMSMEWLGLGITPASYDNGYDIQKDRQNFFLDTYTRQAIAYCLD
                     RQTIATSVLHGLTEVPTSYLPLGHPALDTNLSAINYDPEIGNSLLDQAGWLDEDENPS
                     TPRRAINAQKVAYNTPLILNYQTTSTTQRRQVAAILEQSLANCGIGLNVEFISPNDLY
                     APGPVGPLFGRQFDLAEYALGVDGSEPPCGWFSTSSIPSEANSWSGTNVTGFSNEEYD
                     AACQAGQSALQGDESYLSAYRQTQIILADQLPAIPLFPRLHIAAARPDLCGFDLDPSA
                     RSLWNAEAFEISETCQN"
     gene            3824..4909
                     /locus_tag="PLG52_05255"
     CDS             3824..4909
                     /locus_tag="PLG52_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB40883.1"
                     /translation="MIRNVLLVDDQRDILRLLRSTLDTLKNKDIQIYEAQSGEEAMLE
                     SSRHKIDLLVTDYKLPGMSGVELMHKVRARHAEVKVIFITGMTDRKMREDMLNSGAFG
                     IFDKPIPIADFLDSVERGLGFVQTIFPPELTAEANALGEGEIQQTKVSDLLANFRQDF
                     NANAVFLLNDRGLINARTGNLRDPSMEVSLISTLMAIHNTSLKVAKVNHQEVVESYHV
                     FSGGDHDLLFIPVTPLYALLVAGKDLATENQILDTVRGLMALRSQVEKALSTLGVTGQ
                     LKFKTAPLAVPPTEAPPKKNTDQLAKATPAPEMEALLSNASGEKAKEKSTDDFWESAA
                     QDLGRKSSNPEVISLEEAKKMGLIPGE"
BASE COUNT         1291 a         1203 c         1151 g         1324 t
ORIGIN      
        1 gtttttcgcg caggtgacca gatctctgaa gtcttaaaca ttcatcaaga ttttggcaaa
       61 gaagctgggc gtgcacgtgc agttgaactg ttgaaacaag tgggtattcc tgagccaaat
      121 cggcgcgcgg aagccttccc tcatgaactc tctggcggta tgtcacagcg tgtcatgatc
      181 gcaatggcac tggcttgtgt ccctgatctt ctgattgcag atgaacccac cacagctctt
      241 gatgtaacca ttcaagcgca gattcttgac ttgatgcgcg acttgcgcaa cgaactcggc
      301 tctgctttga tgctcattac acatgacctc ggcgtgatcg ctgaaatggc gaatcgtgtt
      361 gttgtgatgt atgcaggcga gatcgtagaa gaatctccgg ttgcttctct ttttgacaaa
      421 ccgcatcacc cttacaccca aggcttgatc ggttccatcc cagttttggg tcaacgtcgt
      481 gaccgtttag atgtcatccc cggaaacgtt ccgaatctcg tcaacctccc gcaaggctgc
      541 cgctttgctc ctcgttgccg cgctcgtaca gaacataacc tttccatttg taccgagaaa
      601 catcctggct tgatccaagt agcagatggg cataaagttc gttgctggtt gtatgaagat
      661 gcggatggac acactgcacc actgaaatct ggtaaataaa ggacgcggct tagatgaata
      721 agataaacaa acaagaacag ccagaactgt tggtggttga taacctcgtc aagtactttc
      781 ctgttcgtac aggtttattt cagcgcgtaa ccgcttgggt tcaagcggtg gacaaagtca
      841 gctttgccgt gaaaaaaggc gaaaccctcg gcatggtggg tgagtctggt tgtggcaaaa
      901 caaccgtagg acgcaccctt ttgcgcctga tcgaaccaac ctctggcaat gtgacattcg
      961 aaggaaagga tgtcttaaaa ctaaatcagc gtgaactaaa acctttacgc cgtgatatgc
     1021 aaatcatctt tcaagaccca tatgcatctc ttaatccacg tatgcctatc ggcgagtcgg
     1081 tcatggaagg tttacaaatt cacaacattg gtcacccgaa agaacgctgg gagatcgctg
     1141 tcaatatgct caaaaaagtg ggcttggaag aataccatgc ccgccgctat ccgcacgaat
     1201 tttcgggcgg tcagcgtcaa cgtataggca tagcccgtgc tttggcattg aatcccaagt
     1261 tcatcgtttg tgatgagccg gtttccgctt tggatgtttc cattcaatct caggtgctta
     1321 acatcctaaa ggaccttcaa gaagagttcg gtctcacata ccttttcatt gcccataacc
     1381 ttagtgtggt ggaacatatc tcagaccgtg tagcggtgat gtatcttggc aaaatggtcg
     1441 agttgactga ccgtgactcg ttgtatcgtg aaccgcttca tccttacacc aaggcattgc
     1501 tttctgccat ccctgtgggg cacccgaatg agaaacggga gcgcactatt ctcaaaggag
     1561 atgtgcccag cccgctgaac ccgccaaagg gttgtcgctt ccatacacgc tgcccaattg
     1621 cagtcagtca gtgttcgcag gaagagccaa cctttaagga agttcgccca ggtcattggg
     1681 tggcttgttt gttagcccaa taaaaaataa agagaccgca cacgcggtct ctttttataa
     1741 taggggagtg gttataatta cgaaacgcct taaacagata tggcaggagc tatgaaaaaa
     1801 aacgtcatcc catttttact tgttattgtt cttattgtga ttccagcctg tttaagtgcg
     1861 cctgcacaga cagaaacccc ggcaccgact gaatcattta tagaaattac tgaaacaccc
     1921 ccgccttctt tgggaccctc acttaccgtc tgtttggggc aagagccgaa tagtttgtat
     1981 ccattcggag atttgaatga tgcggctcgt aatgtcctct ctgcgatcta tgacggaccg
     2041 attgataatg tgggctatga atatcagcca gtgattctca ccaaacttcc ttctattgaa
     2101 aatggcgatg cgcagattgt tcctataaag ataaataatg gggactctgt tgttggtgcc
     2161 gatgggaata ttattcagtt cagtaaaggc tccattgtgc gccctgctgg ctgccgtaat
     2221 gctgactgtg ccatcacata cgatggtacc tcttctatcg aaatggacca aatgattgtg
     2281 acatttcgcc tacgccccga cctgacttgg tctgacggca ctccactcac agccgatgat
     2341 tccatctatt cgtttgaact gcaaaaagaa agcaaagtca atacatatct caccgagcgc
     2401 actcaaacct acgaagccgc cgatgagcaa accctgcaat ggttcagtat tccggggtat
     2461 ctcgacccga cctacttttt gaatttttgg cagcctgctc cgaaacatgc ttggagcgaa
     2521 ttcacgcctg accaattgcc cactgttgat gtctcttccc gtgagccatt aggatggggt
     2581 ccttacaccg tcaaagaatg gttgccaggc gaaggactca cgctcgaaaa gaacccttac
     2641 tattaccggt ctcgcgatgg gtatcctaaa ttggatagca ttcaattccg cttcattccc
     2701 gacccagatc tggctctgtc cgaattaatt gcaggtcgct gtgacttgat cgaccctacc
     2761 attaaccttg aagacaaagt tggattgctg caagaaatgc agacttctgg tcaggcaaag
     2821 ttttttgcaa ccactggcat gagcatggaa tggcttggct tgggaattac acctgcttct
     2881 tatgataacg gctatgatat tcagaaggac cgccaaaact ttttcttgga tacatatacc
     2941 cgccaagcca ttgcttactg tttagaccgc caaactatag ccacgtccgt tctccatgga
     3001 ctgacggaag ttccaaccag ttatttacca ttgggtcacc ccgcgttgga tacaaatctc
     3061 tctgccatta actatgaccc cgaaattgga aattctttgt tagatcaagc aggctggttg
     3121 gatgaagatg aaaacccttc cacgccacgc cgggcgatca atgcccaaaa agtggcttat
     3181 aacaccccgc ttattttgaa ctatcaaacc acctccacta cccaacgcag gcaagtagcc
     3241 gccattttgg agcaatctct tgcaaactgt ggaattggat tgaacgtcga attcatatcg
     3301 cccaacgacc tatatgctcc tggtccggtt ggtccattat ttggtcgcca atttgaccta
     3361 gccgaatatg ctctcggtgt ggatggtagc gaaccacctt gtggttggtt cagtacgtca
     3421 tcaattccaa gtgaggcgaa ctcatggtct ggtacaaatg taacaggctt tagtaatgag
     3481 gaatacgatg ccgcctgtca ggcggggcaa tccgccttac agggagatga atcttatcta
     3541 tcagcatatc gccagactca gatcattttg gcggatcagt tgccagccat cccgcttttc
     3601 cctcgcttgc acatagcggc tgctcgcccc gacctttgcg gtttcgacct tgacccatct
     3661 gcccggtcgc tttggaatgc cgaggcattt gagatcagcg aaacctgtca aaactgattg
     3721 ggaagcaatt cctgaatctt gctataattt agacatcgtt atcaagtttg gatgttatta
     3781 atccaaacga atttattcca ttgatttcaa acaacaggct ggtatgattc gaaacgtttt
     3841 gttagtggac gaccaacgcg atattctacg acttttgcgt tccaccttgg acacgctcaa
     3901 gaataaggat attcagatct acgaagccca atcaggggaa gaggctatgt tggagtcttc
     3961 ccgtcataaa atagatcttt tagtgactga ttacaaactt cccggcatga gtggcgtgga
     4021 gttaatgcat aaagttcgtg ctcgtcatgc agaagtaaaa gttattttca ttaccggcat
     4081 gaccgaccgc aaaatgcgcg aagatatgct caacagcggc gcttttggca tctttgataa
     4141 acctatccca attgccgatt ttctagactc tgttgaacgt ggacttggtt ttgtacaaac
     4201 catctttcca ccggagctta ccgctgaagc gaatgcactt ggcgaagggg aaatacaaca
     4261 gacgaaagtt tctgacttgc ttgccaattt caggcaggat ttcaatgcca atgctgtatt
     4321 tctgcttaac gatcgcggtt tgattaatgc ccgcaccggg aaccttcgtg accccagtat
     4381 ggaagtctca ttgatttcca ctttgatggc gattcataat accagtttga aagtggcaaa
     4441 ggttaatcat caagaagtgg ttgagtccta tcatgtcttt agtgggggag accatgattt
     4501 gctgtttatc ccagtgactc cgctttatgc gttgcttgtc gctggaaagg acttggcaac
     4561 tgaaaatcaa attctggata ccgtgcgtgg cttgatggct ttgcgttctc aggtagagaa
     4621 agcattatcc acattagggg ttaccggaca attaaagttt aaaactgcac cactagcagt
     4681 tccgcctaca gaagcacctc caaagaagaa tactgaccag ttagccaaag caacccccgc
     4741 tccagaaatg gaggcgttgc tgagtaatgc ttctggcgag aaagccaagg aaaagtcgac
     4801 agatgatttt tgggagtctg cggcgcaaga cttgggtaga aagtctagta accctgaagt
     4861 gatctctttg gaagaagcta aaaaaatggg acttattccc ggcgaataaa aaaattgtga
     4921 tacaatcttg cccgcactgg ggcatggtgc aatggcagca caccagcct
//
LOCUS       DAOQNJ010000133         4961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10274_length_4961_cov_7.151651, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000133 DAOQNJ010000000
VERSION     DAOQNJ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10274_length_4961_cov_7.151651"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PLG52_05260"
     CDS             complement(<1..1035)
                     /locus_tag="PLG52_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB40884.1"
                     /translation="MQRTFAVFIALSMTISACGASPSIWGVVPTPTPNASQTAPLTDP
                     FAGQSDPIVFPTSTIPPFIETENAQTPTPQVTEETVTTQVEPSITTAPLNDAAPFLYY
                     AQSGDMLSAVAARFGVSESEIISDADLTKTTLIDPGTLLVIPNRINEPTTPNVQLLPD
                     AEFVFSATSIGFDTEKFVKDQNGYLSSFRDYLGSVGWVQGYDAIDRLSVENSVSPRLL
                     LALLEYEARWVRGQPIDLLHTEFPMGFNDYHYKGMSVQMTWAINNMSIAYYGWRAGTI
                     THIEFPDGTRLRLDPRLNAGTVAIQYLFSKLHSESQWSQIINPDSGFPALYNEMFGDP
                     WARADLVGPIF"
     gene            complement(1077..2684)
                     /locus_tag="PLG52_05265"
     CDS             complement(1077..2684)
                     /locus_tag="PLG52_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB40885.1"
                     /translation="MSFRKTVSVFLLAILIVSCAPATEEPATFPTYNPFVSTNGATLP
                     PNSNVAVTTPLSSETVIPSATSTRPPTPTRVPLNISSLIGVQSQIISGSPTPDTARVL
                     PTPRQDADQYVVQAGDTLGAIAQRYNITLQTLMDANGITDPNALEVGMTLNIPAAQVT
                     VSSGQSLKIIPDSELVNGPATAKFSLRNFIASQNGYLNYYTQDVNGTLLNGADIIEAV
                     SYNYSVNPRILLALLEYQSGWVTNPTPTNIDYPMGYYDEARAGLYRQVVWAANTLNRG
                     YYLWKANGLSTWVLTDGQVLPIDPTINAGTAAVQYFFSQLYGYDAWQYATSQTGVVVT
                     YFVLFENPFNYAIEPLIPATLTQPQMSLPFAAGESWYFTGGPHGAWDSGSAWGALDFA
                     PPDNSGGCNISSSWVTAIADGLVTRTGTGSVYQDLDNDGYEQTGWVIIYMHVASAGRV
                     TPGEYLFRNEKVGHPSCEGGISNATHVHITRKYNGEWIAADGPYPWNLDGWISSGTGY
                     EYDGFLTRNGVSIEALDAAQPANLISR"
     gene            complement(2762..3208)
                     /locus_tag="PLG52_05270"
     CDS             complement(2762..3208)
                     /locus_tag="PLG52_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB40886.1"
                     /translation="MTTVRKLLEAKEFATNYSVETNQTVLDALKVMAEAHIGAVLVTD
                     GGKIVGIYTERDYLYKGEALGRVAKDTKIKDVMTPKMINVTMETTVEQCMGLMKQYKI
                     RHLPVVEGEHFVGLVSMRDVMLAAIDIKESEIRGLENYIMGSGFQS"
     gene            complement(3346..3852)
                     /locus_tag="PLG52_05275"
     CDS             complement(3346..3852)
                     /locus_tag="PLG52_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNB40887.1"
                     /translation="MKKILLLGYGNPDREDDGVAWHILRALAVRMGLPAPDSYEDDFP
                     ENSQVNFAFYLQLTPEMAEDIAQYEYVCFVDAHTGNIPEEVRIIDVESDFQKSPFTHH
                     LTAQSLMSICETVYHRKPDAALLSVRGYQFLFSRLLSTPTAALVPEAVDLAWAWINAR
                     EPLKQAGD"
     gene            complement(3910..4356)
                     /locus_tag="PLG52_05280"
     CDS             complement(3910..4356)
                     /locus_tag="PLG52_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB40888.1"
                     /translation="MTTVRKIIDEKSRAEVYTISANENVMRALEEMSKSNVGALLVIE
                     DGKITGIFTERDYARQGELKGRLAKDTLVREMMSRDLVMVNPETLLSECAELMYTHHV
                     RHLPIVEKGQVVGVVSIRRLAEALIEQEKGNVTKLEHYILGTGYGE"
     gene            complement(4411..>4961)
                     /locus_tag="PLG52_05285"
     CDS             complement(4411..>4961)
                     /locus_tag="PLG52_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNB40889.1"
                     /translation="LGRLNLAEKIDTPLANEELKIFKSLSNGKMVENTLYYHYARLIE
                     ALFAAERVRVLCEDKDITSTDILNTRKNYTGHGVGVIEAPRGTLIHDYTTDENGKLLK
                     VNLIVSTGHNNWAMTTAVDSVAKTYVKGPDVHEGMLNRVEAAIRAYDPCLSCSTHAVG
                     QMPIQVDFIAPDGTMIKTLKRD"
BASE COUNT         1243 a         1145 c         1277 g         1296 t
ORIGIN      
        1 gaaaatggga ccgactaaat ctgcgcgtgc ccaagggtca ccgaacattt cgttgtacaa
       61 ggctgggaag ccagagtccg gattgataat ttgtgaccac tgggattcac tgtgaagctt
      121 tgaaaataaa tattgaatgg caacggtgcc tgcattgaga cgagggtcaa ggcggagtct
      181 tgtaccgtcg gggaattcaa tatgcgttat ggtgccagcg cgccaaccgt aataggcaat
      241 cgacatgttg ttgattgccc atgtcatctg tacgctcatg cctttgtaat gatagtcgtt
      301 aaagcccatt ggaaattcag tgtgtaacaa gtctatgggc tgaccgcgca cccagcgcgc
      361 ttcgtattca agcaaagcca gcaacaagcg cgggctgaca gagttctcga cggaaagacg
      421 atcaatggca tcgtaacctt gtacccaacc gacagaccct aagtaatcac gaaacgaact
      481 gagatacccg ttttggtctt taacaaattt ttctgtatca aaacctatag acgtggcaga
      541 gaaaacaaat tcagcatctg gcaaaagctg aacattgggc gttgtgggtt cgttgatgcg
      601 gtttggaatg acaagcaggg tgccggggtc gattagggtt gttttggtga gatctgcatc
      661 tgaaataatc tcagattcgc tcacaccaaa gcgagctgca acggcagaga gcatatctcc
      721 gctttgggca tagtatagaa aaggtgcggc atcattaagc ggagccgtgg taatggatgg
      781 ttccacttgg gttgtaacag tttcttcagt aacttgtggt gttggcgttt gtgcattctc
      841 cgtctcaatg aaaggcggaa tggttgaggt ggggaaaacg atcgggtcac tctgaccagc
      901 gaacgggtcc gtaagaggcg ctgtttgaga ggcgtttggg gttggggtgg gaacaactcc
      961 ccaaatggaa ggcgaagccc cacaggcaga aatggtcatc gatagtgcga tgaatacggc
     1021 aaaggtacgt tgcatggttt gagtgattat aaccagttag gttaatatca agtgagttag
     1081 cgtgaaatga ggttggctgg ttgagccgca tctaaggctt caatactaac gccgttacga
     1141 gtgaggaagc catcatattc atagccggtg ccgctggata tccaaccatc aaggttccaa
     1201 gggtagggac catctgcggc aatccattcg ccgttgtatt tgcgtgtgat atggacatgc
     1261 gtggcgttcg agatgccgcc ttcacaagaa gggtgtccca ctttttcatt gcggaaaaga
     1321 tattcgccgg gtgtaacacg ccctgcggat gcgacatgca tgtaaataat gacccagccg
     1381 gtttgttcgt agccatcatt atcaaggtct tgatataccg agccggtacc cgtacgagtg
     1441 acgagaccat ctgcaatagc ggtgacccaa ctggagctga tgttacaacc gccactatta
     1501 tctggcgggg caaaatcgag cgcgccccaa gcagaaccgg agtcccaggc accgtgtgga
     1561 ccacccgtga agtaccagct ttcgccagct gcaaacggca gagacatttg cggctgtgtg
     1621 agtgtggcag ggattaatgg ttcaatggca tagttgaatg gattttcaaa aaggacaaag
     1681 tatgtgacca ctacaccggt ttggcttgta gcgtattgcc aggcatcgta accataaagc
     1741 tgtgagaaga aatattgtac agcggctgtg ccagcgttga ttgtagggtc aatgggaagc
     1801 acctgaccat ctgttaacac ccaagtagaa agcccattgg ctttccagag ataataaccg
     1861 cggtttagtg tgtttgccgc ccaaaccacc tgccgatata acccggcacg ggcttcgtcg
     1921 taatacccca tggggtaatc gatattggtt ggtgttgggt tggtaaccca gccgctttga
     1981 tattcaagca aggcaagtaa aatgcgcggg ttgacggaat agttataaga cacggcttca
     2041 ataatatctg cgccgtttaa taaagttcca ttcacgtctt gggtgtagta attcaggtag
     2101 ccattctggc tggcaatgaa attgcgtaaa ctgaattttg ccgtagccgg tccgttgacc
     2161 agttcggagt ctggaataat tttgagggat tgtccgcttg atacagtaac ttgagcggct
     2221 ggaatgttta atgtcatgcc aacttctaat gcatttgggt cggtgatgcc attggcatcc
     2281 ataagagttt gaagggtgat gttataacgt tgagcgatag cacctaaggt atcaccagcc
     2341 tggacgacat attgatccgc atcttggcgc ggagtgggca acacccgagc cgtatctgga
     2401 gtaggtgagc cggagataat ctgactttgt acaccaatga gagaagaaat gttcaaaggc
     2461 acacgagtag gcgtgggcgg gcgcgtggag gttgctgacg gaataaccgt ttcagaagac
     2521 agaggcgtgg ttaccgccac gttgctattt ggcggaaggg ttgctccatt tgtagaaacg
     2581 aaagggttat aggtagggaa agttgcaggt tcttctgtgg caggtgcgca agaaacgatg
     2641 agaatagcaa gtaaaaacac agatacagtt ttgcgaaaag acataaaagt gattttacca
     2701 aatcgtatcg gtaaaaaaag aggcgcaaac attgcgcctc ttttcaagtt tgttgagaga
     2761 actaggactg aaaaccagac cccatgatgt aattttcaag cccgcgaatt tcgctttctt
     2821 tgatgtcaat cgcagccagc atcacatcac gcatagaaac caaacctaca aaatgttcgc
     2881 cttccactac aggtaagtgg cggatcttat actgtttcat cagccccatg cattgctcaa
     2941 cagtagtttc catggttacg ttgatcattt tcggtgtcat cacatctttg atctttgtat
     3001 ctttggcaac ccgccccaac gcttcccctt tgtagagata atcacgttca gtataaattc
     3061 caacgatctt tccgccatct gttaccaaca cagctccaat atgggcttca gccatcacct
     3121 tgagggcatc caataccgtc tgattggttt caacagaata gtttgtcgca aattccttag
     3181 cttccagaag ttttcggaca gtggtcatga ttttcgtctc cttatataag ttctttttta
     3241 tcataggcaa aataaaacgt cacacggaaa acctcctcat ctcgcgtgac gtttgtcatc
     3301 tgatttattt ttgacccgtc tgcactcttt gctttcggct catccttaat ctccagcctg
     3361 tttcagcggt tctcgggcat ttatccacgc ccaagctaag tccacagctt cgggcacaag
     3421 agcagctgtc ggcgtggaaa ggaggcgcga gaacagaaat tgatatccgc gcaccgagag
     3481 caatgctgca tctggtttac gatgatagac tgtttcgcag atagacatca aagactgggc
     3541 ggtgaggtga tgcgtgaatg gcgatttctg aaaatcgctt tctacatcaa taatgcgtac
     3601 ttcttctgga atgttgcccg tgtgcgcatc cacaaagcag acgtattcat attgtgcaat
     3661 gtcttccgcc atttctggtg tgagttgcag gtaaaaagcg aaatttacct gtgagttttc
     3721 agggaaatca tcctcgtacg aatccggtgc aggcagcccc attcggaccg ccaacgcccg
     3781 caggatgtgc caggcaacac catcatcttc tcggtcgggg tttccgtagc ctagtaataa
     3841 aatctttttc attcaacctc taaaaccttg ataaacctcc gaggtcttta agacctcgga
     3901 ggtttgggat tattcgccat atcctgtgcc gaggatgtaa tgctcaagct ttgtgacatt
     3961 gcctttttcc tgttcgatca aggcttcagc caacctgcga atggacacga caccaacaac
     4021 ctgtcctttt tccacgatgg gcaggtgacg gacgtgatgc gtgtacatca attcggcgca
     4081 ctccgagagc agtgtttccg ggttaaccat caccagatct ctactcatca tctcacggac
     4141 aagcgtatct tttgccaacc tgcctttgag ttccccttga cgagcgtaat cgcgttcagt
     4201 gaagatgccg gtgatttttc catcttctat tactaggaga gcgccaacat tggacttact
     4261 catttcctcc aatgctctca taacattctc gttagcagag atggtgtaaa cctcggcacg
     4321 agatttttca tcgatgatct ttcgtacagt cgtcatggtt aaactccttt ggtgtatcaa
     4381 agtgaacaat ggacgacgaa ccactaaacc ttaatcgcgt ttcagggtct taatcattgt
     4441 accgtctgga gcaataaagt caacttggat gggcatctgc ccgacggcat gcgtggaaca
     4501 ggacaggcac gggtcatagg cgcggatggc ggcttcgacg cggttgagca tcccttcgtg
     4561 gacgtctggt cccttgacgt aggtcttggc gacgctgtcc acggcggtgg tcatcgccca
     4621 gttattgtga ccggttgaaa cgatcaaatt caccttcagc aatttgccat tctcgtctgt
     4681 cgtgtaatcg tggatgagtg ttccgcgtgg cgcttcgatc acgcccacgc cgtgaccggt
     4741 gtaattcttg cgggtgttga ggatgtcggt gctggtgatg tctttgtctt cgcacagcac
     4801 acgcactcgc tccgccgcga acaaggcttc gatcagacgg gcgtagtgat agtacagagt
     4861 gttctcgacc atcttgccgt tgctgaggct cttgaagatc tttagctctt cattcgccag
     4921 cggagtatcg atcttctcgg cgaggttcaa acgtcccagc g
//
LOCUS       DAOQNJ010000134         4945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10329_length_4945_cov_6.719018, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000134 DAOQNJ010000000
VERSION     DAOQNJ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10329_length_4945_cov_6.719018"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /gene="mtaB"
                     /locus_tag="PLG52_05290"
     CDS             complement(<1..1178)
                     /gene="mtaB"
                     /locus_tag="PLG52_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNB40890.1"
                     /translation="MKVFLDTIGCRLNQAEIESMSRQFRAAGHEIVASAENADMAVVN
                     TCAVTTQAASDSRGKIRTIARAGVNEIVATGCWTSIQPKEAAALPNVKHVISNDKKDL
                     LVPEILGLPKEKFDVEPIDRIPIPGLHRRTRAFIKVQDGCDNKCTFCVTTIARGEGRS
                     RPLADVINDVNSALAGGSKEIVLTGVHLGSWGQDFGNHLRELVQALLHETDVQRLRLS
                     SLEPWDLDVDFFSLWNDKRLMPHLHLPLQSGSDSTLKRMLRKTTTASFRELVHAARSI
                     LPDVAITTDIIAGFPGETDEEFAETMEFVQEMRFTGGHVFSYSPRPGTGAAKMKGQVK
                     PEVRKRRNRILQEVIEESAKSYREKFIGQTMRVLWESTTEYGEYGWRMEGWTGNYLRV
                     "
     gene            complement(1197..1850)
                     /locus_tag="PLG52_05295"
     CDS             complement(1197..1850)
                     /locus_tag="PLG52_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40891.1"
                     /translation="MNRSVSLVFVLMFLLSACGGAPANQTQAPAAALPSGSVLFQDDF
                     SQPTTGWDRLLVTEGVMDYYSGGYRMLVNSLQTNFWATPRRNFTDVRMEVDTGKLAGP
                     DENRVGLLCRYNGKDYYFFIMTSDGFYGIGLYKDGQATLLGQSEMLASSNINKGVAVN
                     HLRADCTGNILTFYINGFQVTQAQDTTLPSGDIGLIAGTFAEPGVDILFDNFVVLQP"
     gene            complement(1864..3258)
                     /locus_tag="PLG52_05300"
     CDS             complement(1864..3258)
                     /locus_tag="PLG52_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB40892.1"
                     /translation="MLLQRAKPYFIGLLLVIAIIIPFLVAGYNSLNRAENAYSTEQYR
                     LAAEEYAHTARILFWRKDLYGRAGISAAQGEDFEGAIAYFKQAKNLTEMGWVLFCQSY
                     AQLQDYPNTISTCKAGLQKYQSAPLYKILAFVYRDQKDWVAELDALKHQTQLDPNDAY
                     AAYRLGVLMALYAPEDSIPELTRASSLNPEVDSAVQTLRAALAVSSQQSDPSMQKVIV
                     GQAFGLVQDWELAKTAFEQAVQQNENNAEAWAWLGEAKQQTGQDGSAELDQALLLDHN
                     SVNVRALRGLYWSRKGKYSQMLAEYSLAARIEPENPRWQASLGDAYSKIGDLVAAMAA
                     YQRAVELDPTQADYWRLLAIFCAENNVHAEDVGIPAAQQAYTLSPNDPASLDALGYAY
                     LSTGRYASAELAFSQAIEISAQYYPAHIHLAMNYLAQGNRAAAFNALTYVRDADGAGV
                     YAETAKQLLDKYFQ"
     gene            3310..>4945
                     /locus_tag="PLG52_05305"
     CDS             3310..>4945
                     /locus_tag="PLG52_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB40893.1"
                     /translation="MSDFIQLALNQLTVPPGDMIYYIVLAFTIASALQSAFNHWRASE
                     FPQARRAFIGLGILLSAQIFMFLFSGLGWQELIDSKAVLPPMDRAFLVFGIVWISWLY
                     AFPEPNRQADAVATLLSMFIMTALGLSLISWQQQMANPATATLSYNLTLDDFIWQIGS
                     VLLALLGMAVLFIRKPDGMWYGLILLFLGFLGHVGQLLIGLEGDFSGIVRLAYITAFP
                     ILLTLPQRFGTVTVVETSAPVKPTILKQDTAGRPERRRYSTDPKTFHALLAVAAENNS
                     TKMSQAITRAIAQTMLADLCFMIYLTDNNNQMVIAGGYDLIREDTLEGGSLSKNAIPM
                     LANSIQRGRPLRLPASSTSADIKGLGDILGLTNPGHLLYVPILTPEKETLGGILLLSP
                     YSDRTWSAEDQAFLSNIASSLVPIIQRNQKITKLEAQSDMARAQVADLEERIRNLNGQ
                     LDAARTEVERNKSTDVASLRVAQEESQNIIEQLQRENAELRSGKNLPSAQAEKELKAT
                     LQEMAILQNQLADANIKLLELEKGKIATKSTEQAEVL"
BASE COUNT         1303 a         1258 c         1138 g         1246 t
ORIGIN      
        1 ctcacacgta gataattacc cgtccagcct tccatgcgcc agccatattc accatattca
       61 gttgtggatt cccacaacac gcgcatggtt tgaccgataa acttttcacg atacgacttt
      121 gccgattctt cgatcacttc ttgcaaaata cgattacgcc tcttgcggac ctcgggcttg
      181 acctgcccct tcatcttcgc cgccccggta ccgggacgtg gtgaatagga gaagacatgc
      241 ccgcccgtga aacgcatttc ttgaacaaac tccatcgtct ctgcaaattc ttcatctgtc
      301 tcgccgggga aaccagcgat gatgtctgtg gtgatcgcca catcaggcaa gatacttcga
      361 gccgcatgaa ccaactcacg gaaggaagct gttgtggtct tacgcagcat ccgcttcagg
      421 gtagagtcac tgccagattg cagaggcagg tgcaggtgag gcatgaggcg cttgtcattc
      481 caaagcgaga aaaaatctac atctaagtcc caaggttcta gagaggataa gcgcaaacgt
      541 tgaacgtccg tttcgtgcaa aagagcttgg actagttcac gaagatggtt gccaaaatct
      601 tgaccccatg agccaagatg cacgcccgtt agaacaattt cttttgaacc acctgccaat
      661 gccgagttga catcattgat cacatctgcc aatggacgcg accgcccttc accacgcgca
      721 atggtagtga cacaaaatgt gcatttgtta tcgcagccat cttgtacttt gatgaaggca
      781 cgggtgcggc gatgcaatcc cggaatagga atgcggtcta ttggctcaac atcaaatttc
      841 tctttgggca agccaaggat ctcaggcact agcaaatctt ttttatcatt ggaaataaca
      901 tgcttgacgt ttggaagtgc cgccgcttct tttggttgta tcgaagtcca acagcctgtt
      961 gcgacgattt cattgacccc agcacgagca atagtacgaa tctttccccg agagtcagat
     1021 gcggcttgag ttgtgactgc gcaagtgtta accactgcca tatctgcatt ctcagcagaa
     1081 gccacaattt catgccccgc cgcacggaac tggcgagaca tggattcaat ttctgcctga
     1141 ttcaaacggc agccaatggt atcgagaaag actttcataa acaggtgtta cgtatattaa
     1201 ggctgcaata caacgaagtt atcaaaaaga atatcaacgc cgggttcggc gaaggttccg
     1261 gcaatcaagc caatatcacc cgaaggcaaa gttgtatctt gagcttgcgt cacctgaaaa
     1321 ccatttatat agaaggttaa gatattcccc gtgcaatcgg ctcgcagatg attgaccgca
     1381 acacctttgt tgatattcga ggaagcaagc atttcagatt gccccaacaa agttgcctga
     1441 ccatctttat acaaaccgat cccgtaaaaa ccatcgctgg tcatgataaa gaaatagtaa
     1501 tctttgccgt tataacggca aagcagaccc acacggtttt cgtctggacc tgctaattta
     1561 ccggtgtcca cttccatgcg gacatcggtg aaattacggc gtggcgttgc ccaaaaattt
     1621 gtctgtaacg aattaacgag catacgatag ccgccagagt aataatccat taccccttcg
     1681 gtcacgagca aacgatccca gccagttgtt ggctgagaaa aatcatcttg aaaaagaaca
     1741 gaccccgaag gcaaggctgc cgctggagcc tgagtttgat ttgcaggggc gccgccgcat
     1801 gcagaaagta aaaacattag aacgaatact aaagatacag atcgattcat gagcgtgatt
     1861 ttatcattga aaatatttgt ctaataactg cttggcggtt tcggcatata cgcctgcgcc
     1921 gtcggcatcg cgcacatacg tcagtgcatt aaaagccgcg gcacgatttc cttgcgcaag
     1981 atagttcatc gcaaggtgaa tatgagccgg ataatactga gccgaaattt caatcgcttg
     2041 tgaaaaagcc aattctgcgc tggcataacg ccctgttgaa agataagcat agcccaaagc
     2101 atccaaagaa gccgggtcgt tgggagaaag ggtataagct tgttgtgcgg caggaatacc
     2161 aacatcttct gcatgtacgt tattctctgc gcaaaagatc gccagcaagc gccaatagtc
     2221 ggcttgtgtg ggatcaagct ctaccgcacg ttgatatgcc gccatagccg ccaccaagtc
     2281 tcctattttg gaataggcat cgcccaaact tgcctgccaa cgcggattct ccggctcgat
     2341 gcgcgccgca agcgaatact ctgccaacat ttgcgaatac ttgcctttgc gactccagta
     2401 cagtccgcgc aaagctcgca cattgaccga attatggtct aggagtaatg cctgatcaag
     2461 ttccgcgctt ccatcttgac ctgtttgctg tttcgcctct ccgagccatg cccacgcctc
     2521 agcgttgttt tcattttgtt gaaccgcttg ctcaaatgct gtttttgcca attcccaatc
     2581 ttgtaccaag ccgaatgcct gccccacgat caccttctgc atagaaggat cgctttgctg
     2641 gctggaaact gctaaagcgg cgcgcaaggt ttggacagca gaatctactt cggggttaag
     2701 agaggaggcg cgtgtcagct ccgggatgga atcttccggg gcgtacaaag ccatcagcac
     2761 cccaagtcga tacgcggcat aggcgtcgtt tgggtctaat tgagtttggt gcttaagtgc
     2821 atcaagttct gccacccaat ctttttgatc tcgatacaca aacgccaaaa ttttatataa
     2881 cggtgcagat tgatatttct gaagccccgc cttgcaagtt gaaattgtgt ttgggtaatc
     2941 ttgcagttgc gcatacgatt ggcaaaacaa gacccacccc atttcagtga ggttcttggc
     3001 ttgtttaaaa tatgcaatgg caccttcaaa atcttcgcct tgcgccgccg aaatacccgc
     3061 acgcccatac agatcctttc gccagaacag tatgcgggca gtatgggcat attcttcagc
     3121 cgcaagccga tattgctcgg tagaatacgc attttcagct cggtttaggc tgttatagcc
     3181 tgccacaagg aacggaatga tgatggcgat gacaagcaaa agacctataa agtagggttt
     3241 ggctctttgt agcagcataa tttcaattct acgccataat ccctaactgg cagtataatc
     3301 acgagcggaa tgagcgattt tatacagtta gcattaaacc aactcactgt tccaccggga
     3361 gatatgatct actacatcgt tctggcgttt accatcgcca gcgcgttgca atctgcattt
     3421 aatcattggc gggcaagtga atttccacaa gcacggcgtg ctttcattgg tctgggcatt
     3481 ttactcagcg cacaaatctt tatgtttctc ttcagcgggc tgggctggca agaattaatc
     3541 gactcgaaag ccgtcttacc acccatggac cgtgcctttt tggttttcgg catcgtgtgg
     3601 atctcttggt tatacgcttt ccccgaaccc aatcgccaag ctgatgcagt tgccaccctt
     3661 ctcagcatgt tcattatgac tgccttgggg ctgagtttaa tctcatggca acagcaaatg
     3721 gcaaaccctg ccactgccac attgagctac aacttaactt tagacgattt tatctggcaa
     3781 attggttctg tactgctcgc cttgttgggc atggctgtgc ttttcatccg caaaccggat
     3841 ggaatgtggt acggattgat cttgttgttt ctcggctttt tagggcatgt cggtcaatta
     3901 ttgattgggc tggaaggcga tttctctgga atcgtccgct tggcgtatat aacggctttc
     3961 cccattttat tgacacttcc acagcgtttc ggcacagtga ccgtggtcga aacgtcggct
     4021 ccggtcaaac cgaccatttt gaaacaagat acagcgggtc gccctgaacg gaggcgttac
     4081 agcacagacc cgaaaacatt ccatgccttg ttggcagttg ccgctgagaa caactccacc
     4141 aagatgagtc aggcaatcac acgtgccatt gcacaaacca tgcttgccga cttgtgcttt
     4201 atgatctacc tgaccgataa caacaaccaa atggtgatcg ccggcgggta tgacttgatc
     4261 cgcgaagaca cattggaagg cggctcgcta tcgaagaatg ccattccaat gctggcgaac
     4321 tccatccaac gcggacgccc cttacgatta cctgccagca gcacctcagc agatatcaaa
     4381 ggcttgggcg atattcttgg gctcaccaac cctgggcatt tgttatatgt ccccatcctc
     4441 accccagaaa aagaaacctt gggcggtatt cttctcttat ctccatactc agaccggacc
     4501 tggagtgctg aagatcaggc tttcctttct aatattgctt cttctttggt accgattatt
     4561 caacgtaacc aaaagattac caagctcgaa gctcaaagcg atatggctcg cgcccaagtt
     4621 gcagatttgg aagaacgcat tcgcaatttg aacggtcaac tcgatgcagc tcgtacagaa
     4681 gttgagcgta ataaatcaac agatgtcgct tcattacgtg tggcacagga agaatcccaa
     4741 aatatcattg agcagttaca gcgcgaaaat gcagaactac gttctgggaa gaacctgcct
     4801 tctgctcaag cagaaaaaga attgaaagca actttgcaag aaatggcaat tttacaaaac
     4861 cagttggcag acgccaatat caagcttctg gaattggaaa aagggaaaat cgctaccaag
     4921 agcaccgagc aagcagaagt gctcg
//
LOCUS       DAOQNJ010000135         4944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10334_length_4944_cov_5.722029, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000135 DAOQNJ010000000
VERSION     DAOQNJ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10334_length_4944_cov_5.722029"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="PLG52_05310"
     CDS             <1..1353
                     /locus_tag="PLG52_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB40894.1"
                     /translation="TPTEKPRGQNPPQGQGSDATQSLDFDIPAHPFDVILGRPTSNSV
                     TFSLLAYTDQTLSISYGTESGNYTSQTENINLTANTPQTFQLSNLQPSTNYFYSINGG
                     SENTFHTARPAGSTFTFTIQADSHLDSNSSAEVYLQTLANQRADNPDFIIDMGDTFMT
                     DKYKPYTDAAPQYLAQRYFMGQLGLPLFLVLGNHDGEGAPRGKDGEAMSLWSAGLRTQ
                     YFPNPVPNDFYSGNITPHPTVGDLQNYYAWEWGDALFIVLDPYWYTKSAPSKDQAADL
                     WNPTLGDAQYQWLKSTLENSNAKWKFVFIHQLIGGLEKDGRGGIEVAPFHEWGGNNPD
                     GTYAFDQYRPGWELPIHQLLLANNVNAVFHGHDHLFVHQELDGIVYQEIPQPGSANPN
                     NTNSAAQYGYVNGTILGGPGHMRITVSPEQVLVEYVRSYIEEKPAQKNGQVDFSYTIK
                     "
     gene            complement(1372..1809)
                     /locus_tag="PLG52_05315"
     CDS             complement(1372..1809)
                     /locus_tag="PLG52_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB40895.1"
                     /translation="MAKHFNDLEQNAWGGFLGTYAKLNRLIEEDLQAHSRLAHVEFEV
                     LLRLSWNESHRMRIQDLAAESILSRSGVSRVVERLEKAGLVTREEAPEDRRGAYAVLT
                     EAGAERFRVAAEKHAECVRSHFTSKFSDDELEVMGAFWKRLEE"
     gene            1899..2291
                     /locus_tag="PLG52_05320"
     CDS             1899..2291
                     /locus_tag="PLG52_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40896.1"
                     /translation="MKTKIPVWINILQVVIFAILTFQTYACYFNPQLLYPGITVDENS
                     AKMIYVLGGRNAMMAIISVIALVRQNPRFYSFAFLMHSLRDFQDMFIAPLTGGGIVVF
                     FVFLIVFVTPEILSYLKLNKMANEMEKA"
     gene            2292..2606
                     /locus_tag="PLG52_05325"
     CDS             2292..2606
                     /locus_tag="PLG52_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB40897.1"
                     /translation="MPDTSTCRQEPGVFIPVIDRNKCEGKADCVPACPYSIFKAETLP
                     ADLRVGLTFIGRLKGTTHGWQQALLVHPEQCRACGLCVKACPEHAITLSRSATFMSPP
                     AR"
     gene            2632..3231
                     /locus_tag="PLG52_05330"
     CDS             2632..3231
                     /locus_tag="PLG52_05330"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNB40898.1"
                     /translation="MTKTRLEAFSDGMFAIILTILVLELHVPELENSSFAAFSAGMIQ
                     LAPKFFAFLFSFFIIAIFWINHHAIIHHIHKVDTKLLWLNMGLLFFSSLFPFITAFIG
                     DYMMNPFVVALYPLNMALSSLTINALWKYAYVDTDLAPNNLTAEEKQARMKRDRMALY
                     INLGSVVLAFIWVPITLTIMVVFPFAFVIPTLLGKKEKE"
     gene            3228..4100
                     /locus_tag="PLG52_05335"
     CDS             3228..4100
                     /locus_tag="PLG52_05335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB40899.1"
                     /translation="MKDKIVLITGATSGIGFQTALALARMDAQVIVTGRSQKSAQEAV
                     AKLKAESKNERVDFLLADLSAQKNVRVLAEQFKARYKRLDILINNAGLATSKYELTED
                     GIESDFAMNVVTPFLLTTLLMDSLKKSSSPRVVTLMGGDVPATLDMDNLRSEKSFDGL
                     NTYSQTKLAMMALMYEFAQREKDATINVCYPGQASTNMTRSVTPEMLPRAMRWMFPIF
                     KLAVREDGGKSAAKAARSSVYLASSPEVDGVTGKYFDTNCKEATWSPAVMDAANRERL
                     WKIVEQITFATKEH"
     gene            complement(4132..4380)
                     /locus_tag="PLG52_05340"
     CDS             complement(4132..4380)
                     /locus_tag="PLG52_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40900.1"
                     /translation="MENLKEWLLGLDTMILVILFLGGFIAWELKSGEVPLRWFGSIRR
                     DARPFFYWIAMLFHLAILGIVIYAYTDGVRMPLAEFFQ"
     gene            4602..4844
                     /locus_tag="PLG52_05345"
     CDS             4602..4844
                     /locus_tag="PLG52_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB40901.1"
                     /translation="MAAKGWIEVSDTYCKGCELCISACPQNVMELNMEHLTPKGYHPA
                     HTFKDGCTGCAICAVVCPDAAITVYREVTKAAPVAA"
BASE COUNT         1300 a         1374 c         1101 g         1169 t
ORIGIN      
        1 acccccactg aaaagccgcg tggacagaat ccgccccaag gtcaaggtag tgacgcgact
       61 caatcgctcg acttcgacat ccccgcccat cctttcgatg taattctcgg acgccccaca
      121 tccaacagtg tcacattttc tttgcttgcg tacaccgacc aaacactttc catttcttat
      181 ggaactgagt cgggcaacta cacatcacaa acggaaaata tcaatctcac ggcgaacacg
      241 ccacaaactt tccaactttc caacctgcaa ccttcaacca actactttta ttccatcaac
      301 ggcggaagcg aaaacacctt ccacaccgcc cgccccgcag gctcaacctt caccttcacc
      361 atccaagccg actcacacct tgactcaaac tccagcgccg aagtctattt gcagaccctc
      421 gccaaccaac gtgccgacaa ccccgacttc atcattgaca tgggtgacac gttcatgacc
      481 gacaaataca aaccctacac cgacgccgcg cctcaatatt tggcacagcg ttacttcatg
      541 ggacagttgg gtttgccgct ctttcttgtg ctgggcaatc atgatggcga aggcgcgccg
      601 cgtggaaaag acggcgaagc catgtccctc tggtcggcgg gactgcgtac tcaatatttt
      661 cccaaccccg taccgaacga tttttattcg ggtaacatca cgccacatcc cacagttggc
      721 gacctgcaaa actactacgc atgggaatgg ggcgacgcgc tcttcatcgt ccttgacccg
      781 tactggtaca ccaaatccgc gccctccaaa gaccaagccg ctgacctatg gaatcccacc
      841 ctcggcgacg cgcaatatca atggctcaaa tccacgctgg aaaacagcaa cgccaaatgg
      901 aagttcgtct tcatccacca actcatcggc ggactggaaa aagacgggcg cggcggaatc
      961 gaagttgctc ccttccacga atggggtggt aacaatcccg atggcacgta cgccttcgac
     1021 caatatcgcc ctggctggga attacccatt catcaattac tgcttgccaa caacgtcaac
     1081 gccgtttttc acgggcacga ccacctcttc gtccatcagg aactcgatgg cattgtctat
     1141 caagaaatcc cccagcccgg ttccgccaac ccaaacaaca ccaacagtgc tgcacaatac
     1201 ggctatgtca acggcaccat cctcggcgga cctggtcaca tgcgtatcac cgtcagccct
     1261 gagcaggtgc ttgtggaata tgttcgctct tacatagaag aaaagcccgc tcaaaagaac
     1321 gggcaggtgg attttagtta caccattaaa tgatctcaca tagtgagggg gttattcttc
     1381 caaccgtttc caaaacgctc ccatcacttc caattcatca tcactgaatt tgctagtgaa
     1441 atgggagcgc acacattctg catgtttttc tgccgccacg cggaagcgct ccgcgcccgc
     1501 ctcagtcagg acagcgtacg cgccgcgcct gtcttcgggc gcttcttcgc gtgtgaccaa
     1561 gcccgccttc tcgagccgct ccaccacacg gctgactccg ctgcgagaga gaatactctc
     1621 cgccgccaaa tcttgaatcc gcattcggtg cgattcgttc cacgaaaggc gcagtaatac
     1681 ttcaaactcg acatgggcga ggcgggagtg cgcctgcaaa tcttcttcaa tcaaacgatt
     1741 gagtttggca tacgttccga gaaagccccc ccaggcattt tgttcaaggt cattaaagtg
     1801 ttttgccata atttctccac atttctctat tgacatttag gtgactacgc acataaaata
     1861 ggtgcgcaat cacctaattt tagcacagga gcaaaaccat gaaaaccaaa attcccgtct
     1921 ggatcaacat tctgcaagtg gtcattttcg ccattctgac cttccaaacc tacgcctgct
     1981 atttcaaccc gcagttgctc tatccaggca ttacggtaga tgaaaactcc gcaaaaatga
     2041 tttacgtcct cggcgggcgc aatgcaatga tggcaatcat ttcggtgatt gcgcttgttc
     2101 gccaaaatcc gcgcttctat tccttcgcct tcctcatgca cagtttgcgc gattttcaag
     2161 atatgttcat cgctccactc acaggcggcg gtatcgtcgt gttctttgtc tttctaatcg
     2221 tgttcgtcac gcctgaaatt ctctcgtacc tcaaactcaa caagatggca aacgagatgg
     2281 aaaaagcgta aatgcccgat acctccacct gccgacaaga gcctggcgta ttcatccctg
     2341 tgattgaccg caacaaatgc gaaggcaaag cggattgcgt acccgcctgc ccatactcca
     2401 tcttcaaagc ggaaacgctt cctgccgacc tgagagttgg gctgactttc atcggcagac
     2461 tcaaagggac aacgcacggg tggcagcaag cattactcgt ccaccctgaa caatgccgag
     2521 cctgcgggct gtgcgtcaaa gcctgccccg aacatgcgat tacgttatct cgtagtgcga
     2581 catttatgtc gccacccgcg agataaatct cgcgctacca taggactacc catgaccaaa
     2641 acccgcctcg aagcctttag cgatggcatg ttcgccatca tcttgactat tctcgtcctc
     2701 gaactgcacg tccctgaact ggagaattct tccttcgccg cattcagcgc agggatgatt
     2761 caactcgcgc cgaaattctt cgctttcttg ttcagtttct tcatcatcgc cattttttgg
     2821 atcaatcacc acgccatcat tcaccacatt cacaaagtgg ataccaaact gttatggcta
     2881 aatatgggct tgctgttttt ctccagcctc ttccctttca tcaccgcttt tatcggcgac
     2941 tacatgatga atccattcgt ggtggcgttg tatccgctca acatggcgct ctcttcgctc
     3001 acgatcaatg ccctatggaa gtatgcttat gtggacactg acctcgcgcc gaacaacctc
     3061 accgccgaag aaaaacaggc gcggatgaaa cgcgaccgaa tggcgttgta tatcaatctt
     3121 ggctcggtgg tattggcgtt tatttgggtt ccgatcacat tgaccattat ggtggttttc
     3181 ccatttgcct ttgttatacc gactttattg ggtaagaagg aaaaggaatg aaagacaaaa
     3241 tcgttcttat cacaggcgcg accagcggaa ttggattcca gacagcactg gctctcgcaa
     3301 gaatggacgc gcaggttatt gttacagggc ggagtcaaaa gtccgcgcaa gaggcggtgg
     3361 cgaaactcaa agctgagagc aaaaacgagc gcgtggattt tctgctggca gatctttcgg
     3421 cgcagaaaaa tgtccgtgtt ttagccgaac aattcaaggc gcgatataag cggcttgata
     3481 tcctgatcaa taacgcaggg ctggcaacat ccaaatacga actgaccgaa gacggaatcg
     3541 aatctgattt tgccatgaac gtggtcacgc catttttgct gaccaccttg ctgatggatt
     3601 ctttgaagaa aagttcttcg ccgcgtgtgg tgacattgat gggcggcgat gtacctgcca
     3661 cgctcgatat ggataacctg cgatcggaaa aatcgttcga cggattgaac acctactcgc
     3721 aaaccaaact ggcgatgatg gcgttgatgt acgaatttgc ccagcgtgaa aaagatgcaa
     3781 cgatcaacgt ttgctaccct gggcaagcca gcacgaacat gacccgcagt gtgacacccg
     3841 aaatgctgcc gcgtgccatg cgttggatgt tccccatttt caaattggct gttcgtgaag
     3901 acggcggtaa atctgcggcg aaagcggcgc gttcgtcagt atatcttgcg tcatcgcccg
     3961 aagtggatgg cgtgacggga aaatatttcg acacgaattg caaagaggcg acgtggtcac
     4021 ctgctgtgat ggatgcggca aaccgcgaaa gattatggaa gatcgttgaa caaattacat
     4081 ttgcgacaaa agaacattga gataaaaaag agccgcaatt gcggctcttt tttattgaaa
     4141 aaattctgct aagggcattc gaacaccatc tgtatatgca tatatcacaa tgcctagaat
     4201 tgctaaatga aacagcatgg cgatccaata aaaaaatgga cgggcatcac gccgaattga
     4261 accaaaccaa cgcaaaggga cttcaccaga ttttaattcc cacgcaataa agccacccaa
     4321 gaaaagaata actaaaatca tcgtgtctaa cccgagtaac cattctttta agttttccat
     4381 agatacactc ctaaattttt tgaaattata cccatctcag agttctgaaa ttgtctgttc
     4441 aagtcgctct taagtccttg tagttgaccg aaaaacgaat gacctttgta ctcgcttgta
     4501 catgacgcct gacactactc ctcgatttcc gaaaacgaca taatagactt agcgatgaaa
     4561 gtcgcctaat agtgtctaac ttctcaatat aaggagctgt gatggcagca aaaggttgga
     4621 tcgaagtcag cgatacctat tgcaaaggct gtgaactttg cataagtgcc tgtccacaaa
     4681 atgtgatgga actcaatatg gagcacctca ctccgaaggg ataccacccc gcgcacacgt
     4741 tcaaagacgg ttgcacgggg tgtgctattt gtgcggtcgt ctgcccagat gcggcgatta
     4801 ccgtctatcg tgaagtgacc aaggctgcgc cggttgcggc gtaattattc gtaagggcga
     4861 ctcgcgagtc gcccttaccg gttacatgga gaaacgaatg ccaaaagaat tatggaaagg
     4921 caatgaagcc atcgcagaat ctgc
//
LOCUS       DAOQNJ010000136         4937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10355_length_4937_cov_7.153626, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000136 DAOQNJ010000000
VERSION     DAOQNJ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10355_length_4937_cov_7.153626"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="PLG52_05350"
     CDS             <1..141
                     /locus_tag="PLG52_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB40902.1"
                     /translation="GHHCAQPLHEKFGIPATSRASFYLYSTKDEVDMLVKGLYKVKEM
                     FG"
     gene            190..585
                     /locus_tag="PLG52_05355"
     CDS             190..585
                     /locus_tag="PLG52_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNB40903.1"
                     /translation="MDDLYREVIIEHYKNPSYRGKLDPHDISFADNNPLCGDHIQIDL
                     RVDANGVVSEARFDGHGCAISQASADLLMETIIGKPLEEVKKLNRQDILDMLGIDLGP
                     VRLKCALLSLKVLKAGVYGLGEAGDDLVE"
     gene            591..1031
                     /locus_tag="PLG52_05360"
     CDS             591..1031
                     /locus_tag="PLG52_05360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40904.1"
                     /translation="MASYQIREMVIDDYDAIIVMFHETPGITVRDADSRESTAAYLTR
                     NPGLSFVATINGKIIGCVMSGHDGRRGYLQHLIVTPEFRRQGVGKALYSACLAALQKI
                     GIYKTHLFVQKSNSSGEEFWREQGWVFRDEVLMFSFNASPNENI"
     gene            1047..1394
                     /locus_tag="PLG52_05365"
     CDS             1047..1394
                     /locus_tag="PLG52_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HNB40905.1"
                     /translation="MFNYTSFDESKVEFVEIAPASELPNGERLFVDLGDKPVVIFNIA
                     GQFFAIGDVCTHDDGPLGDGMLENFNVVCPRHGAEFDIRTGQAVQMPAVVDIPAYPVQ
                     VRDGTIFIGLPKE"
     gene            complement(1450..>4937)
                     /locus_tag="PLG52_05370"
     CDS             complement(1450..>4937)
                     /locus_tag="PLG52_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HNB40906.1"
                     /translation="IILFIVIILLAIVTRFYNLGARVMSHDESLHTYFSWLLYQGRGY
                     EHTPMMHGPLQFHLIALSYFLFGASDYTARIPAVLFNLMTIWMVWYWRRYLGRTGALI
                     AGFLLVISPFMLYYGRYVREDSYPIFSGILMLYAVLRHLESGGKKYLYILSAALLIHY
                     VAKETSFIYTAQLLIYLAIYFIVQVSRTPWKGATSDYRTFIIALCVTILLVGATAGYV
                     LYSRTPETTNAAQTAVPANPDAAAQTSPESALSPLTIILGIAAVLAFVVTAYFLIRGY
                     TWERLRSNRAFEMLMITGTLVLPQLSPLLINLTKVTIPTTAPEITALAGDWRAILTIG
                     ACLLVTFLLAIAIGMIWNPKTWWKATLIFWVPYVILYTAIFTNPNGFFTGTIGSLGYW
                     IVQHDVQRGSQPWYYYLLIQVPVYEFLPALGLILALILGLRRKVSLPAAYEEDMIDGT
                     FDGNWEEGKQPAPTQEQQEAYTREMNFVNMFSLLVWWSFITFFAFSFAGERMPWLTIH
                     ITLPLILITGWALGSIIDRTDWSALKEKQAWLGLAAVIIFVLSAANVIYAWNSPSRPF
                     QGTSLEQLQATNLFLLPLIVCIVSAGASVYLLRSWSSKDVRHVFTIVVFAFLGVLTVR
                     ASFRAAYLTYDQATEYLVYAHGATGIKDVINQATEISKRTTGGMDIAIAYDASAPDTG
                     VSWPFVWYLRDFTNQRSFDQPTRALRDSVIVIVDEKNFDKIDQALGPGYYRVDYIRMW
                     WPMQDYFGLVSDRDPSIPFDENYSCKGLLGFLKLVKSKDYSRFCEGFTDPQIRAGILQ
                     IWLNRDYTLYAQVKGRSDLTLATWQPADQMRMYIRKDVAAQIWNYGVAPTADEVTVDP
                     TEGKYVTLAADKILDATQPNPIVLNAPRALAFAPDGTLYIADSLNHRILHVDAEGNPL
                     HTWGTFADGVSVQIGDGTFNEPWGVAVGPDGSVYVSDTWNHRIEKFTATGTFIKAWGT
                     FGQGETPDSFYGPRGLATDAEGRVYVTDTGNKRIVVFDADGNYITEFGSAGFDPGQFD
                     EPTSVAVDRNGTVYVTDTWNQRIQTFKRVETPEGGLSFEPEKQWDVYGWFGQSLDNKP
                     YIAVNDQLHVFVNDPEGYRVMEFDQDGQIVRVWGDYSNANTGFGLASGVAVDPDGHVW
                     VSDSAFNRVMRFTLP"
BASE COUNT         1312 a         1083 c         1341 g         1201 t
ORIGIN      
        1 gggcatcact gtgctcagcc tttgcatgaa aagtttggca tccctgctac cagccgagct
       61 tctttctatt tgtactctac aaaagatgaa gtggatatgc tggtaaaggg tttatataaa
      121 gtaaaagaaa tgttcgggta attatggagc gaggtctcct cgccctgttt ccctgaaacg
      181 gaatatataa tggacgatct ctatcgtgaa gtaatcattg agcactataa aaatccatcc
      241 tatcgtggaa agttagaccc gcacgatatt tcctttgcag ataacaaccc attatgcggc
      301 gaccatatcc aaatcgattt gcgagtggat gcgaatggtg ttgttagcga ggcgcggttt
      361 gatgggcatg gttgtgcgat ctctcaagcc tcagcagacc tgttaatgga gactattatc
      421 gggaagccgc tggaagaagt aaaaaagctc aaccggcagg atattcttga tatgctcggt
      481 attgacctcg gaccggtgcg tttgaagtgt gcattgcttt cgcttaaagt gttgaaggct
      541 ggagtatatg gcttagggga agccggagac gatttggtgg agtaagaagc gtggcttcat
      601 atcagatccg cgagatggtc attgatgatt acgatgccat cattgtgatg tttcacgaga
      661 cgccgggcat cacagtacga gatgcagact cgcgagaatc aacagccgct tatttgacta
      721 gaaaccccgg gctgagtttc gtagccacca taaatggaaa gataatcggc tgcgtaatgt
      781 ctggacatga cgggcggcgc ggctatttgc aacacctgat cgtaacgcct gaatttcgaa
      841 gacaaggcgt tggtaaggct ttgtattcag cttgccttgc tgcccttcag aaaatcggta
      901 tctataagac ccatcttttt gtacaaaagt cgaattcttc tggagaagag ttttggagag
      961 aacagggctg ggtctttcga gatgaagtgc tgatgttttc tttcaatgca tctccaaacg
     1021 aaaatatatg aaacctgaga acaaccatgt ttaattacac ctcctttgat gaatcaaaag
     1081 ttgaatttgt agaaattgcc cctgcttccg aactcccgaa tggggaacgc ctctttgttg
     1141 atttaggcga taagcctgtt gtgatattca atatcgcggg tcaattcttt gcaattggtg
     1201 acgtctgcac ccatgatgat ggtccgttag gagatgggat gcttgagaac tttaatgtgg
     1261 tctgtccgcg acacggcgct gagtttgaca ttcgcaccgg gcaagccgtt caaatgcctg
     1321 ccgtggtgga tatccctgcc tacccggttc aagtccgcga tggaacaata tttattggct
     1381 tgccgaagga ataagtttac ctaataaaaa agcccctcaa ataaattgag gggctttttt
     1441 ataaaaagtt tacgggagtg taaatcgcat gacgcggttg aatgccgagt cactaaccca
     1501 tacgtgccca tcagggtcaa cagcaacacc tgatgccaac ccaaagccgg tgtttgcatt
     1561 gctatagtcg ccccatacgc ggacaatctg accgtcttgg tcaaactcca taacgcgata
     1621 cccttcgggg tcattcacaa acacatgcaa ctgatcattc acagcgatgt agggtttgtt
     1681 gtcgagcgat tgcccaaacc aaccgtatac atcccactgt ttttcaggct cgaaggataa
     1741 gccgccttca ggggtttcta ctcgcttgaa ggtttgaata cgctgattcc atgtatcggt
     1801 tacatagacc gttccattac ggtcaacagc gacactggtc ggctcatcga attgaccggg
     1861 atcgaagccg gcggaaccga actcggtaat gtagttcccg tccgcatcaa acacgacgat
     1921 acgcttgttc cctgtatctg tgacatagac tcgcccttcg gcatctgttg ccaaaccgcg
     1981 aggaccgtag aatgagtcgg gggtttcgcc ttgcccgaaa gtgccccatg cctttatgaa
     2041 ggttcctgtg gctgtgaact tttcaatgcg gtgattccaa gtatcagaga catataccga
     2101 tccatccggc ccaactgcca cgccccatgg ctcattgaaa gtgccatcac cgatctgcac
     2161 actgacacca tctgcaaagg tgccccaggt gtgaagcgga tttccttctg catccacatg
     2221 caaaatgcga tgattgagag agtctgcaat gtaaagggtg ccatctggtg caaatgccaa
     2281 agcgcgcgga gcatttaata caattgggtt gggctgagta gcgtcaagga tcttatcggc
     2341 tgccaatgta acgtacttgc cttcggtggg gtcaactgtg acttcatctg cggtgggggc
     2401 aacgccataa ttccagatct gagcggcaac atccttacga atgtacatgc gcatttggtc
     2461 agcgggctgc caggtagcca gcgtcaggtc agagcgtccc tttacctgag catacaaggt
     2521 gtaatcgcgg ttcagccaga tttggagaat acccgcgcgt atttgaggat cagtgaagcc
     2581 ttcacagaaa cgtgaataat ctttggattt tacaagtttc aagaacccga gcagaccctt
     2641 acaagagtaa ttctcatcaa aggggatgct tgggtcgcgg tcgctaacga gaccgaaata
     2701 gtcttgcatg ggccaccaca tgcgaatgta atctacacgg taatagccgg gacctaaagc
     2761 ctgatcgatc ttgtcgaagt ttttctcatc gacgatgacg atgactgagt cgcgtagggc
     2821 gcgagtgggt tggtcaaatg aacgttgatt ggtgaagtca cgcagatacc aaacgaaggg
     2881 ccaagagacg ccagtatccg gtgctgaggc gtcgtaggca atggctatat ccatgccgcc
     2941 tgtggtgcgt ttggaaattt ctgtggcttg attgatgaca tccttaatgc ccgtcgcgcc
     3001 gtgagcatag acgagatatt ctgtggcttg gtcataggtg aggtaagcag cacggaagga
     3061 ggctcgaacg gttaagaccc cgaggaaggc aaaaacgacg atggtgaaaa cgtgacgaac
     3121 gtcttttgaa gaccaactgc gtaaaagata tacagaggct ccggcactga caatgcagac
     3181 aattaatgga agcaggaaaa ggtttgttgc ttgtagttgc tccaagcttg tgccttggaa
     3241 agggcgagaa ggggagttcc aggcataaat cacgtttgcg gcgcttaaga caaaaatgat
     3301 gaccgccgcc agaccaagcc aggcttgctt ctctttaagt gcagaccaat ctgtcctatc
     3361 aataatggac cctaacgccc agcccgtgat gaggattaaa ggaagagtaa tgtgaattgt
     3421 gagccagggc attctttcgc cagcgaagga gaaggcgaag aatgtgatga aactccacca
     3481 tactaaaagg ctgaacatat tcacgaaatt catttcgcgg gtgtaagctt cttgctgttc
     3541 ttgggtagga gcaggttgtt tgccttcttc ccaatttccg tcgaatgtgc cgtcgatcat
     3601 atcttcttca tatgcggcag gaagagatac tttacggcga agccccaaga tcaaggcgag
     3661 gatcaagcct agtgccggta ggaattcgta caccggaact tgaatgagaa ggtagtaata
     3721 ccaaggttga ctgccgcgct gcacatcgtg ttgaacaatc caatacccta acgagccgat
     3781 ggtgcccgtg aagaaaccgt tggggttggt aaagatcgcg gtgtataaga ttacgtaagg
     3841 tacccaaaag atcagggttg ctttccacca tgttttgggg ttccagatca ttccaatggc
     3901 aattgcgagt aggaaggtta caagtagaca ggcgccaatt gtgagaatag cacgccaatc
     3961 tccggctaga gctgtgattt caggtgcggt ggttgggatg gtaactttgg tgaggtttat
     4021 aagcagtggg gaaagttgcg gaagaacaag ggtgcctgtg atcatcagca tttcaaaggc
     4081 acggttcgag cggagccgtt cccatgtgta gccgcggatt aagaagtaag cggtgacaac
     4141 gaatgcgagt acagcagcta tcccgaggat aatggtcaat ggagagagcg cagactcggg
     4201 ggaggtttga gccgcggcat ctgggttggc ggggacggct gtttgcgctg cgttggttgt
     4261 ttctggcgtg cggctgtaaa gcacatatcc ggcagtggca ccgactagga gaatagtgac
     4321 acagagtgca atgataaatg tgcggtaatc actggtggcg cctttccaag gggtgcgtga
     4381 gacctgaaca atgaagtaaa ttgccagata gatcagcaat tgtgctgtat agatgaagga
     4441 ggtctctttt gcgacataat gaatgagcaa tgccgctgag aggatgtata gatatttttt
     4501 gccgccgctt tcaagatgcc tgagaacggc gtataacatc aagatgccgg aaaagatcgg
     4561 gtagctatct tcgcggacat aacgcccgta gtaaagcatg aaaggcgaga tgactaaaag
     4621 gaagcctgca atgagagcgc ccgtgcgacc taaataccgg cgccaatacc agaccatcca
     4681 aatggtcatg aggttgaata gaacggcagg gatacgagcg gtgtagtcgc tggcaccgaa
     4741 gaggaaataa gaaagcgcga tcaggtgaaa ctgcagcggt ccatgcatca tgggggtgtg
     4801 ttcatagccg cgcccttgat acagaagcca ggagaaatag gtgtgtaagc tttcgtcgtg
     4861 gctcatgaca cgtgcgccaa ggttgtaaaa gcgcgtgacg atggcaagta agatgatcac
     4921 aataaatagg atgatct
//
LOCUS       DAOQNJ010000137         4936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10359_length_4936_cov_7.569965, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000137 DAOQNJ010000000
VERSION     DAOQNJ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10359_length_4936_cov_7.569965"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PLG52_05375"
     CDS             complement(<1..1034)
                     /locus_tag="PLG52_05375"
                     /inference="COORDINATES: protein motif:HMM:NF003700.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HNB40907.1"
                     /translation="MIRHEEILNTVDMIQKENLDVRAVTMGISLLDCHRSTIEDTCAA
                     IKAKIKHHAAHLVETCESISAEYSIPVVNKRIAVTPVAHVGAGFTAADFVKIAIALDE
                     AAQEVGVDFLGGFSADVSGGTSSGDHELILSIPEALTSTKKVCASINVGTTRSGINMD
                     AVVMLGKTVKELAERSSDQGGFAAAKFVIFTNQPGDNPFMAGAVHGLGQPEVVINVGV
                     SGPGVIARALERRIAQDGSENLGLHELAEEIKRTAFRVTRSGELIGRRVAKALNVPFG
                     VVDLSLAPTPNVGDSVGEIIQILGVDAVGAPGSTAIVAMLNDAVKKGGAFASQSVGGL
                     SGAFIPVCED"
     gene            complement(1044..1619)
                     /locus_tag="PLG52_05380"
     CDS             complement(1044..1619)
                     /locus_tag="PLG52_05380"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNB40908.1"
                     /translation="MTEQQTHFVISITSRDRVGIIHEVTKAISELGGNIADIRQSVLS
                     GYFTMILLASFPPNTTQRAIERKLAEADSNSESAIEAVVRTAEEQLIVSTSMPENAYV
                     LTATGHDRIGFVATMASFCAEHKINIIDLSTTMSDGAYVMILIVDLNKIVSISDMRRD
                     LQEFSQANGIKVVLQHYDIFRAVNEINLPIR"
     gene            complement(1673..2494)
                     /locus_tag="PLG52_05385"
     CDS             complement(1673..2494)
                     /locus_tag="PLG52_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HNB40909.1"
                     /translation="MTEHTSITVVKLGGTEGVDFSAICNDAVELLKQGKKLIFVHGGS
                     AEANALGEGLGTPPKMITSPSGYTSRYTDRKTLEIFLMAVNGKVNSLLTEQLQMLGVN
                     AFGLCGLDGKLMQATRKESVQSIENGKRKIIRDDYTGKIDKVNSELLEMLINAGYLPV
                     IAPVAVSEKGEALNVDADRAAAMVASALRAENLLLLTAVPGLMKNFPDESTLIRQLPM
                     SQLVAASEAAQGRMKKKVLGAEEALKGGVSRVIIADGRIQNPISNALAGNGTVIQ"
     gene            complement(2545..3579)
                     /gene="argC"
                     /locus_tag="PLG52_05390"
     CDS             complement(2545..3579)
                     /gene="argC"
                     /locus_tag="PLG52_05390"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNB40910.1"
                     /translation="MTTVSIIGGSGYGGGELIRLLLGHPNVEIKQVTSRSRVGEYVYQ
                     THPNLRKRTQLKFSDPSQLEPVDILFLAQPHGQAQKNIEEYAKLGKKIIDLAADFRLR
                     DADFYEKWYGEKHSASEWLSKFVYGLPELHREEISKASYISGVGCNATASNLALLPLV
                     KANLLDLSSPIYIEIKVGSSEGGAEGNSGSLHAERANVIRTFSAFGHRHTAEVIQEMG
                     VKDVSLTMTAVDLVRGVLATAHAKVKPGVATKDLWKAYRSVTNENPFVRVVKEQRGIY
                     RVPEPKILAGSNYADLGFELDESNGHLVAMCAIDNLMKGASGTAVQCMNLMMGWDETL
                     GLEFMGLHPI"
     gene            complement(3658..4527)
                     /gene="lysX"
                     /locus_tag="PLG52_05395"
     CDS             complement(3658..4527)
                     /gene="lysX"
                     /locus_tag="PLG52_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HNB40911.1"
                     /translation="MQRRLKIGVLYSRVRVEEKWIFGAMEKRGIDYDRLDDRAIHFDL
                     EKPEIWQQYDAVLERSISYNSGLYALRLLNAFGVPTVNKASVAEICGDKLMTSAVLAR
                     DGVPQPHNVTAFSPEAALDAIEAFGYPVVLKPVVGSWGRLLAKINDRDAAEAVLEHKS
                     TLGSVQHSVFYIQEFIEKPGRDIRIIMIGDKVLTGMYRSSEHWITNTARGGSGELCPI
                     TPEIEDICQRASKAVGGGLLGVDLVEHPEKGLVVNEINHTMEFHTMQPVSGIDIAGEI
                     VDYVVKVAKESNQ"
     gene            complement(4571..4741)
                     /gene="lysW"
                     /locus_tag="PLG52_05400"
     CDS             complement(4571..4741)
                     /gene="lysW"
                     /locus_tag="PLG52_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HNB40912.1"
                     /translation="MSTVTCPECEAQIELVAGTEAGEIIVCSDCGVDLEVTSVDPAAV
                     QLAPMEQEDWGE"
     gene            complement(4745..>4936)
                     /locus_tag="PLG52_05405"
     CDS             complement(4745..>4936)
                     /locus_tag="PLG52_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNB40913.1"
                     /translation="RAAGEAKVRMEELPLEAYQVISPVFEADVYQVFDPLESINKRNT
                     VGGTSLQSVKNQISKIKGE"
BASE COUNT         1241 a         1425 c         1020 g         1250 t
ORIGIN      
        1 tgatcttcac aaaccggaat aaacgcgccg ctcaacccgc cgacgctttg actggcaaac
       61 gcgccgcctt tcttcaccgc atcgttgagc attgctacga tcgccgttga acccggcgcg
      121 cccaccgcat ccacaccgag gatttgaatg atctccccca cactgtcgcc cacattcgga
      181 gttggcgcaa gggataaatc caccacgccg aagggaacat tgagcgcctt cgccacgcgg
      241 cgaccaatca actcaccgct acgagtcaca cggaatgccg tacgtttgat ctcttctgca
      301 agttcatgta aacctaaatt ctcactgcca tcttgagcaa tacgccgttc aagcgcacgc
      361 gcaatcacac ctggtccact gacgcccacg ttgataacca cttcgggttg accgagtcca
      421 tgcacggcac ctgccataaa tggattatca ccgggctggt tggtaaagat gacgaacttc
      481 gccgccgcaa acccgccctg atcactgctt cgttccgcga gttctttgac cgtcttccca
      541 agcatgacca ccgcatccat gttgatgccg gaacgagttg tgcctacatt gatagaagca
      601 cagacttttt tagtagatgt caaggcttca gggatggaaa ggatcaattc atgatccccg
      661 cttgaggtcc cgcctgagac gtcggcggag aaaccgccca gaaagtctac gcccacttcc
      721 tgtgccgctt catccaacgc aatggcaatc tttacaaaat ctgccgccgt aaagcccgca
      781 cccacatgcg ccacaggcgt gaccgcaatg cgtttgttca ccactggaat ggaatattca
      841 gcgctgatgc tttcgcaggt ttcaaccaaa tgagccgcgt gatgtttgat cttcgccttg
      901 atggcagcac aagtatcttc aatagttgaa cggtgacaat caagcaaact aatgcccatc
      961 gtcacggcac gcacatccag attttctttc tggatcatat ctactgtgtt gagaatttct
     1021 tcgtgtcgga tcattttttc tctctatcga atgggcaaat taatttcatt gaccgctcta
     1081 aaaatatcat aatgttgcag cacaaccttg ataccgttcg cctgcgaaaa ttcttgcagg
     1141 tcacgccgca tatcactgat ggatacaatc ttattcaagt caacgatcaa gatcatcaca
     1201 tatgctccgt cagacatggt ggtcgaaagg tcgatgatgt tgatcttgtg ctcggcacaa
     1261 aacgaagcca tggttgcaac gaagccaatg cggtcgtgtc cggtagcggt gagcacatag
     1321 gcattctcag gcattgacgt agatactatc aactgctctt cggctgtacg caccactgct
     1381 tcaatcgcag actcgctgtt tgaatcagct tcggcgagtt tccgttcaat cgcacgctga
     1441 gtcgtgttcg gcgggaaaga agcgagcaag atcatggtga agtatccgct caacacgctt
     1501 tggcgaatat ccgcaatatt gccgcctaat tcgctaattg ctttggtcac ttcatgaatg
     1561 atgcccacac ggtcacgtga tgtgatggag ataacaaaat gagtttgctg ttcagtcatt
     1621 aggggtcgct atcctttgag aaattcagga gattataata tgtctaaaca atttactgga
     1681 taactgtccc attcccagcc agcgcattcg aaatcggatt ctgaattcgc ccatcggcaa
     1741 tgatgacgcg actcacgccg cccttgagcg cttcttctgc acccaacact ttcttcttca
     1801 tgcgaccctg cgccgcctca ctcgccgcca caagttgact catcggcagc tgccgaataa
     1861 gcgttgactc gtctggaaaa tttttcatca acccaggcac cgccgtgagt aacaataaat
     1921 tttctgccct gagcgccgat gccaccatcg cagccgcacg atctgcatcc acattcaacg
     1981 cctcaccttt ttcgctgaca gccactggcg caatgacagg taaataccca gcgttgatta
     2041 acatctccag taattcacta ttcactttat caattttccc cgtgtaatca tcacggataa
     2101 tcttgcgctt gccattttca atagactgca cagactcttt gcgcgtggct tgcataagtt
     2161 ttccatccaa cccacataaa ccaaaagcat tcacacctaa catctgtaat tgttctgtga
     2221 gtaatgaatt aacttttcca ttcacagcca tcaaaaagat ttcaagtgtc ttcctatcgg
     2281 tgtagcgtga ggtgtaccct gaaggtgacg tgatcatttt gggcggcgtg ccaagacctt
     2341 cgcccagcgc gttcgcttct gctgagccgc catgcacgaa gatcaatttt ttgccttgct
     2401 tcaacaactc cacagcatcg ttacagatcg cagaaaaatc cacgccttcg gtaccgccga
     2461 gtttgactac ggttattgat gtatgttctg tcatttttct ccagtacgat actcgaaatt
     2521 cgaattatcc aatatcgaat atcgctatat cggatgtaac cccataaatt caagacccag
     2581 cgtctcatcc caacccatca tcaaattcat acactgcact gctgtaccag acgcaccctt
     2641 cataagatta tcaatcgcac acatcgccac gaggtgaccg ttactctcgt ccagttcaaa
     2701 tcccaaatcc gcataatttg agccagccag aatcttcggc tctggcacac gataaattcc
     2761 gcgctgttcc ttcaccaccc gcacgaacgg attttcattc gtcaccgatc tataagcttt
     2821 ccacaaatct tttgtcgcta caccaggctt gaccttggca tgagccgtcg ccagcacacc
     2881 gcgaaccaag tccacagcgg tcatggtcaa tgaaacatct ttgacgccca tttcctgaat
     2941 cacttcggcg gtgtgacgat gaccgaacgc cgaaaatgtg cggatgacgt tcgcccgctc
     3001 cgcgtgaagc gacccggaat tcccctccgc accgccttcg gaggaaccga cttttatttc
     3061 gatataaatt ggcgaagata aatccaacag attagctttg accaacggca acaacgcaag
     3121 attgctcgcc gtggcattgc atcccacccc actgatataa cttgcctttg aaatttcttc
     3181 acggtgcaat tcgggaagtc catacacgaa cttgctcaac cactctgacg cggaatgttt
     3241 ttcgccgtac catttttcat agaaatccgc atctctcaag cggaagtctg cggcgaggtc
     3301 aataattttc ttgccaagtt tggcatattc ttcaatgttc ttttgcgcct gtccatgcgg
     3361 ctgggcgagg aagagaatat ccaccggctc caactgtgaa gggtcgctaa acttcaactg
     3421 cgtgcgtttg cgcaagttgg gatgcgtctg atacacatac tctcccactc gcgagcgtga
     3481 agtgacttgc ttaatttcca cgttcgggtg acccagcagc aaacgaatca actcgccgcc
     3541 accatatccc gaaccaccaa taatcgaaac agttgtcatt aataattgct cctcattcta
     3601 gtaaccagaa gccagtaaac agtttccagt tgactgcact ggtcactgga ttcttaatca
     3661 ctggttactt tcttttgcca ctttcacgac ataatccacg atctcaccgg caatatcaat
     3721 tccgctcaca ggctgcatgg tatgaaactc catggtgtgg ttgatctcgt taaccacgag
     3781 tcccttttcg ggatgttcca ccaagtccac gccgagcaaa ccgccaccca ccgcctttga
     3841 tgcgcgttga caaatatctt cgatctcggg cgtgattggg cacagttcac cacttccacc
     3901 gcgagccgtg ttcgtaatcc aatgctctga actgcgatac ataccggtca gcactttatc
     3961 gccaatcatg atgatgcgga tatcgcgccc cggtttctca atgaattcct gaatatagaa
     4021 aacagagtgt tgaacagaac ccaaagtcga tttatgttcc aacaccgctt cagcggcatc
     4081 gcggtcattg attttcgcca acaagcgtcc ccacgaaccg acaaccggtt tcaacacgac
     4141 tgggtagcca aatgcctcaa tagcatccaa agccgcttcc ggtgaaaatg cagtcacatt
     4201 atgcggctgt ggcacaccat cacgcgcaag cacggcgctg gtcatcaact tgtcgccaca
     4261 gatctccgcc accgaagcct tattcaccgt tggcacacca aaggcgttca acaatcgcag
     4321 cgcgtacagc ccgctgttgt aactgatact gcgttccaac accgcatcat attgctgcca
     4381 gatttctggt ttctctaaat caaaatgaat ggcgcggtca tcgagccggt cataatcaat
     4441 accgcgcttt tccatcgcgc cgaaaatcca tttttcttca acacgaaccc gtgaataaag
     4501 aacgccaatc tttaatcgtc gttgcataag tttctccgta ggggcgactc gcggtcgccc
     4561 ctacatcaaa ttattctccc cagtcttcct gctccatcgg cgcaagttgc acagcagcag
     4621 ggtcaactga ggtcacttcc aaatccacac cgcaatcaga acagacaata atttcaccgg
     4681 cttcggttcc agcaaccaat tcgatctggg cttcacattc gggacaagta acagtagaca
     4741 taatttattc tcctttgatt tttgaaattt gatttttgac agactggagg cttgtgccac
     4801 caaccgtatt tcttttgttg atcgattcaa gcggatcgaa aacttgatat acatccgcct
     4861 caaacaccgg acttatgact tgatacgctt cgagaggcag ttcctccatc cgaaccttcg
     4921 cctcccccgc cgcacg
//
LOCUS       DAOQNJ010000138         4854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10628_length_4854_cov_10.022088, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000138 DAOQNJ010000000
VERSION     DAOQNJ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10628_length_4854_cov_10.022088"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLG52_05410"
     CDS             <1..201
                     /locus_tag="PLG52_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNB40914.1"
                     /translation="ACSVERHITLDRAMWGSDHAASVEPGGFEKLVKYIRVTEAGLGD
                     GVKRVYESEKAPMKKLRRVVNE"
     gene            249..1103
                     /locus_tag="PLG52_05415"
     CDS             249..1103
                     /locus_tag="PLG52_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNB40915.1"
                     /translation="MGIKQKLRRALRPYAKTAQAIWHWKRFSFNDAPPIFANSKPKSG
                     SHLLLQVMNGFTQIMPYKYVDADPVRTITYNGRRKTNEEILADLKHTPKGVIGWGYVD
                     ATPENASFLTSAGRVNYFIYRDPRDMLVSQVFFATDMHEEHGMHDYYNSLPDFGARLN
                     VAITGIDRDGLKMVSVKQRYEGVFQWLEQKNTMCLRFEDLINNRDATLLKMLDEVEKT
                     GYKIPTPREKALPILVEAIQPKKSHTFRSGKTGGWAQHFTAEHKKLFKDVAGDLLVRL
                     GYEKNNDW"
     gene            1174..2778
                     /locus_tag="PLG52_05420"
     CDS             1174..2778
                     /locus_tag="PLG52_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40916.1"
                     /translation="MKEFFTQRIKRGLVRRLTLLKVASVAREVVHKEPQPSGAPIVFF
                     KASTGIDDLSWNSGFHLLTTWALRLQGIPVAYFACNSGLSKCVLGTNRDNPHKEMPCK
                     SCVMQSKALYTGVEGHKSKVGWFDFQRDPELVSRISSLSVSELSTFHFQDLPLGELCL
                     PALRWILRIHHLNDDENTRYLLREYILSAWNVAQKFSKFLDETSPRLVVVFNGQFYPE
                     ATARYLAKKRGLRVITHEVGLEPATAYFTEGEATAYPIHIPDEFELSEAQNARLDAYL
                     AKRFKGDFTMAGVKFWADMKGLDEAFLQKAAQFKQIVPIFTNVIFDTSQPHSNTVFED
                     MFAWLDTTLEVIKEHPETLFIIRAHPDELRLRKASRETVGGWVESTRVTSLPNVVFVP
                     PNETLSSYELIAKSKFVMIYNSTIGLEASIMGAAVLCAGKARFTQYPTVFFPQTVEDV
                     RRKMKEFLTAETIKVPVEFKRNARRFLYYQLYRTSLPLGQFLEPSVRVTQTKLKTFDL
                     AELQKSKSMQAILDGLLNNGDFLLKE"
     gene            complement(2805..3260)
                     /locus_tag="PLG52_05425"
     CDS             complement(2805..3260)
                     /locus_tag="PLG52_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNB40917.1"
                     /translation="MIIDRIENIALYVSLHPRFKQAFDYLSQIDIHAISVGRHDVDGD
                     AIYALVQEYTTKQTGEGKWEAHRRYIDLQYVVQGAEGMGYANIHHLQQGGYVPEKDFL
                     PLFGEGDQVPLRSGNFVLLFPEDAHMPNMALGEPAPARKIVLKIAVEAG"
     gene            complement(3261..3866)
                     /locus_tag="PLG52_05430"
     CDS             complement(3261..3866)
                     /locus_tag="PLG52_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB40918.1"
                     /translation="MKLTIFNTPIISPLLRLLSRLIMRLVGWRVDGKMPNLSKYVIIA
                     APHTSNWDFLLFLGIVFHLGISVRYMGKAELFNNPFGWFFYWCGGVPVDRKKSVGLVD
                     QMVEVCNQSDKFILVIAPEGTRYQVNEWKRGFYHIAKSANIPIVTARVDGIQKIAHVG
                     QIFQPSDDIEADMKAIQESFIGMTGINPRLKTKKKYITLAN"
     gene            3941..4789
                     /locus_tag="PLG52_05435"
     CDS             3941..4789
                     /locus_tag="PLG52_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNB40919.1"
                     /translation="MALRSFLRSTIYHTEKLAQRLRFSSQSANLPVLLGISFPKSGTH
                     LLDQILLGFSNVAPYAKHVHSMFANYDGESGRRRGADETMAWLHSLSSCDIASAHLFS
                     LPEAVEYVCSPAFVPYFIYRDPRDIVVSHVFYVTDMEPNHVQHQYFKSLPDFNTRLKT
                     SILGRPDAEVEFFDIGTRFKAYADWLDQPSVMKIHFEDLINNRTDTLNHIIDHLLERV
                     PLQASRQLILNSLETSINPTKSPTFRSGKTGEWKKHFTEEHKKIFKEVAGDLLIKLGY
                     EKDNNW"
BASE COUNT         1272 a         1170 c         1095 g         1317 t
ORIGIN      
        1 gcctgctctg tggaacgtca cattacttta gaccgcgcca tgtggggcag tgaccatgcc
       61 gcctctgtgg aacctggtgg ttttgagaag ctggtcaaat acattcgtgt caccgaagcc
      121 gggcttggtg atggcgtgaa gcgtgtgtat gaatctgaaa aagctccaat gaagaaactt
      181 cgccgtgtgg taaacgaata aagaattttg taggggcgag gttacctcgc ccctatattt
      241 attaaattat gggaattaaa caaaaactcc gccgcgcact ccgcccgtat gcaaagaccg
      301 cccaagccat ctggcattgg aagcggtttt cgtttaacga cgctccgccc atctttgcca
      361 actcaaagcc caagagcggt tcgcatttgc ttttgcaggt gatgaatggc ttcacgcaga
      421 tcatgcccta caagtatgtg gatgccgacc ccgtgcgaac gattacctac aacggcagaa
      481 gaaaaacaaa cgaagaaatt cttgcggatc tgaagcacac cccaaaggga gtgatcggtt
      541 ggggctatgt ggatgccacc ccagagaacg cctctttctt gacctcagcc gggcgcgtga
      601 actattttat ttatcgcgac ccgagagata tgctcgtctc gcaagtgttc ttcgcgacag
      661 atatgcatga agaacatggc atgcacgatt actacaattc cctgcctgat tttggtgcga
      721 ggctcaatgt tgccattacc ggcattgacc gtgacgggct gaagatggtt agcgtgaagc
      781 agaggtatga aggcgtcttc cagtggttgg agcaaaaaaa tacgatgtgt cttcgttttg
      841 aagacctcat caacaaccgc gatgcgacct tgttgaaaat gctcgatgaa gtggaaaaga
      901 ccggctacaa gatccccact ccgcgtgaaa aagcattacc tattttggtg gaagccattc
      961 agccaaagaa aagtcacacc ttccgctcgg gcaaaaccgg cggttgggct cagcacttca
     1021 ccgcagaaca taagaagctt ttcaaggatg tagcaggaga tttgctggtc aggttagggt
     1081 acgaaaagaa caacgactgg tagtatatgc atgggactgg agaaattcag tcccattttt
     1141 tatgtactgc tctctgatac aatttgcacg actatgaaag aattcttcac tcaacgcatc
     1201 aaacgcggac tcgtccgcag actcaccctc ctcaaagttg catccgttgc gcgagaggtg
     1261 gttcataaag agccgcaacc cagtggcgca ccaattgttt tcttcaaagc ctccactggc
     1321 attgatgacc tctcatggaa tagtgggttc catttgttga ccacttgggc tttgcgcttg
     1381 cagggcatcc ctgtggcata ttttgcttgt aactcagggc tgagcaaatg cgtattgggt
     1441 acgaaccgcg ataatcccca taaagaaatg ccttgtaaat cttgtgtgat gcagagcaag
     1501 gcgctttata caggagtcga aggtcacaag tcaaaagtcg gttggtttga ttttcagcgt
     1561 gatcctgaac tcgtttctcg tatctcatca ctttctgtct ctgaactttc cactttccac
     1621 tttcaagacc ttcctcttgg cgaattgtgc ttgcccgccc tgcgctggat tcttcgtatt
     1681 catcacttga atgatgacga aaacacccgt tatcttttgc gtgaatacat tttgtccgca
     1741 tggaatgtgg cgcaaaagtt ctcgaagttt ctcgatgaga caagtccacg tttggtggtg
     1801 gtcttcaatg gacagttcta ccccgaagcg acggcacggt atctcgccaa aaaacgcgga
     1861 ctgcgcgtca tcacccatga ggtgggcttg gagcctgcta ctgcctattt caccgaaggc
     1921 gaagcgacag cctacccgat tcatatcccc gatgaatttg agctaagcga ggcgcaaaat
     1981 gcacggctcg atgcttatct tgccaaacgc ttcaaaggtg actttacgat ggcgggcgtc
     2041 aaattttggg ctgatatgaa aggactggac gaagccttcc ttcaaaaagc ggctcagttc
     2101 aaacaaatcg tgcccatctt tactaatgtt atctttgata ccagtcagcc acattccaac
     2161 acggtctttg aagatatgtt tgcttggtta gatacgactc tggaggtcat caaagaacac
     2221 ccagagacct tgttcattat ccgcgctcac cccgatgagc tacgtcttcg caaagccagc
     2281 cgtgaaacag tgggcggatg ggtggaatct acccgagtca ccagtttgcc gaatgttgtt
     2341 tttgttccgc caaacgaaac gctaagttcc tatgaattaa tcgcgaaatc taaatttgtc
     2401 atgatctata actcgaccat tggcttggaa gcttctatta tgggggctgc ggtcttgtgt
     2461 gctggtaagg cgcgttttac tcaatatccc accgtattct tcccgcaaac agtggaggat
     2521 gtacgaagaa agatgaaaga gtttttgacg gctgagacga tcaaagtccc tgtcgaattc
     2581 aaacgcaacg cacgtcgctt cttgtattat cagttatatc gaacctcttt acccttaggt
     2641 caatttcttg agccgtcggt gcgtgtgact caaacgaagt taaagacgtt tgatttggct
     2701 gagctacaga aatctaagtc tatgcaggca atattagatg gcttgttgaa taacggggat
     2761 ttcttactaa aagagtaggg cggggtttgt gccacgaagc ggtattaccc cgcctctact
     2821 gcaattttca aaacaatctt tctagcaggt gcaggctcac ctaatgccat attgggcata
     2881 tgagcatctt cggggaatag caatacaaag ttaccacttc ttaacggcac ttgatcacct
     2941 tcgccaaata aaggcagaaa gtccttctca ggtacatatc ccccttgttg tagatgatgg
     3001 atattagcgt agcccatgcc ttctgcgcct tgcaccacat attgcaagtc gatatagcgg
     3061 cgatgagctt cccattttcc ttcccctgtt tgtttggtcg tgtattcctg aaccaaagcg
     3121 tagatggcgt caccatctac atcatgccgc ccaacggata tcgcatgaat atcgatctgg
     3181 ctcaagtaat caaatgcctg cttgaaacgc ggatgaagcg aaacatatag ggcaatgttt
     3241 tcaattcggt ctatgatcat ttaatttgcc aacgtaatat attttttctt tgtctttaag
     3301 cgtggattga tgcccgtcat gccaatgaaa gattcctgaa tcgctttcat atctgcttca
     3361 atatcatcac taggctgaaa tatctgaccc acatgtgcaa tcttttgaat gccatccacc
     3421 cttgccgtca caatgggaat gttggcgctc ttggcgatat gataaaaccc acgcttccat
     3481 tcgttgacct gataacgcgt cccttcaggg gcgatgacca aaataaattt atcagattgg
     3541 ttgcaaactt ccaccatttg atcgaccagc ccaacagact tttttctgtc cacaggcaca
     3601 ccgccacacc aataaaagaa ccatccaaaa ggattgttga acaactcggc tttgcccata
     3661 tacctcacac taatgccaag gtgaaagaca atgccaagga acagcaaaaa atcccaattc
     3721 gaagtatgag gcgccgcaat aatcacgtat ttagacaaat tcggcatctt tccgtccacg
     3781 cgccatccta ctaggcgcat gatcaggcgg ctcaacaaac gaagaagtgg gctaatgatt
     3841 ggagtgttga aaatggttag tttcactggg gtatcctcat ggcttaaaac cctgaacttg
     3901 tgtttgcgtc tcgcctatca atttgtggtt aaatacctgt atggctttac gatcctttct
     3961 ccgctccact atctatcata ctgaaaaact tgctcaacgg ctacgttttt catctcaatc
     4021 ggcaaatcta cccgttctac tcggaatttc cttcccgaaa agcggtacgc atttgctcga
     4081 ccaaattttg ttgggatttt ccaatgttgc gccatatgcc aaacatgtgc attctatgtt
     4141 tgcaaactat gatggcgagt ctggtcgccg gcgtggtgcc gatgaaacaa tggcatggct
     4201 gcattctttg agttcatgcg atatcgcttc ggcgcatctt ttttcgttgc ctgaagccgt
     4261 agagtatgtt tgcagtccgg cgtttgtccc ttattttatt tatcgtgacc cgcgtgatat
     4321 tgttgtttct catgtgtttt atgtaacgga catggagcca aaccatgtcc aacatcaata
     4381 ctttaaatct ctgccagatt tcaatacgcg cttgaaaacc agtattcttg gaaggccgga
     4441 cgctgaagtg gaattctttg atattggaac ccgcttcaag gcgtatgcag attggctgga
     4501 ccaaccttct gtgatgaaaa ttcactttga agatttgatc aacaaccgta ccgacacttt
     4561 aaaccacatt attgaccatc tcttggaacg agttccgctt caagcttctc gacagttgat
     4621 cctaaattcc ctcgaaacct ccatcaaccc gacaaagtct ccgaccttcc gctctggtaa
     4681 gaccggtgaa tggaaaaaac acttcaccga agagcataaa aaaatcttca aagaagtggc
     4741 tggagatttg ttgattaagc ttggatatga aaaagataac aactggtagg ggcgaggttt
     4801 cctcgcccta ttgataaaaa tgaattgctc catcgtcatc cgcgcctata acga
//
LOCUS       DAOQNJ010000139         4816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10768_length_4816_cov_9.929217, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000139 DAOQNJ010000000
VERSION     DAOQNJ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10768_length_4816_cov_9.929217"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLG52_05440"
     CDS             complement(<1..227)
                     /locus_tag="PLG52_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNB40920.1"
                     /translation="MRHTHGLIEVVCGSMFSGKTDELIRRLVRATIAKQKVQVFKPAI
                     DIRYAVEKVASHTGSTFDAIPVEKASEILSKV"
     gene            complement(329..625)
                     /locus_tag="PLG52_05445"
     CDS             complement(329..625)
                     /locus_tag="PLG52_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNB40921.1"
                     /translation="MEEQKTREESTNVNNRSVDDLGLTPRATEGLKRAGILNIGQLVE
                     KFAEGDAALLAINGFGQSALTAAKKKLRAMDITLPEPPKVEKAEKAEKPAEAAE"
     gene            complement(635..838)
                     /gene="rpmE"
                     /locus_tag="PLG52_05450"
                     /pseudo
     CDS             complement(635..838)
                     /gene="rpmE"
                     /locus_tag="PLG52_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            complement(838..1098)
                     /gene="rpmA"
                     /locus_tag="PLG52_05455"
     CDS             complement(838..1098)
                     /gene="rpmA"
                     /locus_tag="PLG52_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNB40922.1"
                     /translation="MAHKVGGGSSRNGRDSNSQRLGVKRYAGQFVLSGNVLVRQRGTR
                     IKPGLNVMVGKDDTLFATADGVVMFDVYRGKKRVNIVPAEVE"
     gene            complement(1119..1427)
                     /gene="rplU"
                     /locus_tag="PLG52_05460"
     CDS             complement(1119..1427)
                     /gene="rplU"
                     /locus_tag="PLG52_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNB40923.1"
                     /translation="MKFAIVESGGKQYRAVEGTTIDVDRLALELGTKFDFERVLLVAD
                     GDNVSVGTPTLSVKVSATVVDHVKGPKTLAFKYRPKKRIRVRGGNRAHYTRLKIESIG
                     "
     gene            complement(1677..2153)
                     /locus_tag="PLG52_05465"
     CDS             complement(1677..2153)
                     /locus_tag="PLG52_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40924.1"
                     /translation="MPTPIKPAVKPGSAIAKPGTVSYVKFSDKLTASVTDIAKIIEQH
                     KEMIDSIQEVAIELTSSIGSLHALTVKYARTANQILDVLLPILKNLPIIPKNVMQLLV
                     NLESLTQKIIDNEKVTAKTITDVNSGLKTGDANKLKAHAGQLKEVTKTITAIIPKA"
     gene            2302..3147
                     /locus_tag="PLG52_05470"
     CDS             2302..3147
                     /locus_tag="PLG52_05470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNB40925.1"
                     /translation="MSDADVRWERIRNILLLAVLVIIGLLIGSAIFPQPVIGVIRLED
                     AIYTYSAQNTIKQIQYAIDHSEVKAVVFVIDSPGGTVVDTESIYMELTRLREKKPVVT
                     VVNGMSASGGYYLSANTDYIFAKPTSLVGNIGVIGYLPPAPFVIEDVITTGPYKLWGA
                     PRDSDMRQVEMIKQGFFDAVRFGRGDKLTAEENVTFSGQIWAGIDALKLGIIDEFGTE
                     SDGAKKAAELAKVANYEIVDLAAPAGIDMSGGFFFMASADGVTLPYPKEAGMYLLYIP
                     PLPVK"
     gene            3160..4614
                     /locus_tag="PLG52_05475"
     CDS             3160..4614
                     /locus_tag="PLG52_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40926.1"
                     /translation="MKRQTIYIALAALILPILLRGLWFYRGLPAQRPEIATPDYASFE
                     RPAVSVSTPDLENVKQLGGSVLIDAAHTNQFTMTEIDSFIEAIRVRGGTVKTITDATM
                     LETELKTSSAYITVSPSSAFAESEVQALKKFADRGGKILAFAEPTRYFLGFDYVSGNP
                     IPYGDVSAVNSLLKGWDISVNNDYLYNTQKNEGNFRNVLFEDFGKSELTFGLNEVALY
                     GSRSVETDSGLILLQGSETNLSSMDDAHHPHTGGAALSEDGSVAVFGDFTFMASPYNM
                     YTDNATLIQNMADFALAGQQQQSLDVFPYLFKQTTVKVYVSSEIEKTSSMVSALGSLQ
                     STMAALNYKLEFVDSVPTSGDAILVGTFDAPDEFDTYLKKSDVEIDADLLSTVAFGEL
                     SSSGNGLLLFNSNKSGNTLVLLAGSSDDVISLLSVMGYGGLSSCLTSEQVAVCSVGSG
                     DYYYDDTSSDVSTDGTTTDSSTEPTPEATSTPAG"
     gene            4623..>4816
                     /locus_tag="PLG52_05480"
     CDS             4623..>4816
                     /locus_tag="PLG52_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNB40927.1"
                     /translation="MSELIQLGEVKGHFAKPEGEGPFPALIVIQEWWGLDAQTKSIAD
                     RFAKEGYLAFAPDLYHGELA"
BASE COUNT         1179 a         1091 c         1256 g         1290 t
ORIGIN      
        1 acttttgaga gaatttcact ggctttttcc acgggaatgg catcgaaggt tgagccggta
       61 tgtgaagcga ctttttccac cgcataacga atgtcgatgg cgggtttaaa gacctgcacc
      121 ttttgcttgg caatggtggc gcgtactagt cgtcgaatca attcatccgt cttgccgcta
      181 aacattgaac cgcaaacaac ttcgatcaag ccgtgggtat gcctcattga aattctcctg
      241 ccccgttcgg ggaaaatgtt tttttcataa aaacaggcag acgcttttgc atctgcctgt
      301 ttagtttacc tgataagaag cggactgact actcagcagc ttcggcgggc ttttcggctt
      361 tttcagcctt ctctaccttg ggaggttcag ggagagtaat gtccattgcg cggagctttt
      421 tcttggcagc cgtcaaagca gactgaccaa aaccgttgat cgcgagaaga gcagcatcgc
      481 cttcagcgaa tttttcaaca agctgaccaa tgttcaaaat gccagcgcgc ttgagacctt
      541 cggtagcacg cggggtgaga ccgagatcat cgacagagcg gttgttgaca ttggtggatt
      601 cttcgcgggt cttctgttct tccacatagg actggcgagc agaaagcttc ttatagaagc
      661 ggtctacctg accttcgaca tcgaggatct tagcagattc accggtgtag aaaggatggc
      721 agttggagca cacgtccacg cgtaattctt tcttggtgga agtagtaatc caagtgttgc
      781 cgcaggaagc acaagtgacc ttggcatcgt acactgtagg atgaatatcc gctctcatta
      841 ttccacctca gccggaacga tgttcacgcg cttcttgccg cgatagacat cgaacatcac
      901 cacaccgtcg gcagtggcga acaaagtatc atctttaccg accatcacgt tcaaacccgg
      961 cttgatgcgg gttccgcgct ggcgcaccaa cacgttgccg gaaagcacaa actgaccggc
     1021 gtagcgctta acacccaagc gctgggaatt gctatctcta ccgttgcggc ttgaaccacc
     1081 gcctacctta tgagccattg tctacctacc tttatttctt aaccgatgga ttcgatcttc
     1141 aaacgggtgt aatgggcgcg attgccgcca cgcacacgga tgcgtttctt cgggcgatac
     1201 ttgaatgcga gggtcttggg tcccttaaca tggtccacca cagtggcaga aactttcaca
     1261 ctgagggttg gtgtacccac ggagacgttg tctccatcag ccacgagcaa aacacgctcg
     1321 aagtcaaact ttgtgcccaa ttcaagggcg aggcgatcca catcgattgt ggtgccttcg
     1381 acggcacgat attgcttgcc gccgctttca acgattgcaa atttcatttt ctattttctc
     1441 cagtcattca cttcgccgag aaagttcgcg gggatcttgc acccttcgcc tacttctggg
     1501 gaagctgggg ttgtaaacaa acaactaccc cggaatctga cctccggggt ggaagcggac
     1561 ggtattatac atgcagagtc tgggacttgt caacgagatt tgggcgaggg agaactgcca
     1621 aggcggggtg tcctgccccg ttgggcgccg caggtttagg tccagaaaga ggcgggctag
     1681 gctttgggga taatagccgt gatggtcttg gtcacttcct tcaattgacc cgcatgcgcc
     1741 ttcaatttat tggcatcgcc tgttttcaag ccagagttga catcggtaat ggtcttggcg
     1801 gtgacctttt cattgtcgat gatcttttgg gtcaggcttt caaggttgac caacaactgc
     1861 atcacattct tggggatgat gggcaggttt ttgagaatag ggagcaacac atcgagaatc
     1921 tgattcgcgg tgcgggcata tttgacggtg agagcatgca gggagccgat ggaagaggtc
     1981 aattcgatgg caacttcttg aatcgagtcg atcatttctt tgtgttgttc aatgatcttt
     2041 gcaatgtctg tgaccgaggc ggttaattta tctgaaaact tgacgtagga tacggtgccc
     2101 ggttttgcaa tggcgcttcc aggttttact gcaggtttga ttggggttgg catgagaaaa
     2161 tacctcctga aaaaatttgt gctgcacatg cagcaagata tgccaaatat actgtatatc
     2221 gtttcgcaaa acaaccttgt tgacaaagca tgttttctcg tatatcctga aagcgaattc
     2281 caacttatag aggagaaacc catgagcgat gcggatgtac gttgggaacg aattcggaac
     2341 atactgctat tggcagtcct tgtaatcata gggttgctaa taggctcggc aatcttccct
     2401 caaccagtca ttggggtgat acggctggaa gatgcgattt atacctattc agcgcaaaac
     2461 actatcaaac agatccaata cgcgattgac cattcagaag tgaaggcggt tgtatttgtg
     2521 atcgatagcc ccggtgggac ggtggtggat acagagtcca tttatatgga actgacccgt
     2581 ttgcgcgaaa agaagccggt ggtgacagtg gtcaatggga tgtctgccag cggtgggtat
     2641 tatctttctg ctaatacaga ctatatcttc gccaagccta cgtcattggt tgggaatatt
     2701 ggtgtgatcg gctacttgcc ccctgcccca tttgtgattg aagatgtcat tacgacggga
     2761 ccgtataagt tatggggtgc gccgcgtgat tcggatatgc gtcaggtgga gatgattaag
     2821 caaggcttct ttgatgcggt ccgctttggg cgtggggata agctgacagc ggaagaaaac
     2881 gtcacgttta gcggacagat ctgggcgggg atcgatgcgc tcaagttggg catcattgac
     2941 gaatttggga cggagagcga tggcgccaag aaagccgctg aattggcaaa agtcgccaac
     3001 tatgaaattg tagatttggc tgctccggct ggtattgata tgtctggcgg ctttttcttt
     3061 atggcaagtg ccgacggcgt gacattgccg tatccaaagg aagcgggcat gtatcttctt
     3121 tatattccgc ctcttccggt taaataatag gaggatgaaa tgaaacgcca aactatttat
     3181 attgcgctcg ctgccttaat cctccccatt ctcttgcgtg gactatggtt ttaccgtggg
     3241 cttcctgcac agcgacctga aattgctacg ccagattatg catcttttga aagaccggct
     3301 gtctctgtga gcacgcctga cttggaaaat gtgaagcaac ttggtggcag tgttttgatc
     3361 gatgctgcgc atacgaatca atttacgatg accgaaattg actcgttcat agaagcgatc
     3421 cgcgtgcgtg gagggacggt caaaacgatc accgatgcga ccatgctgga aactgagcta
     3481 aagacttcaa gcgcgtacat aaccgtctcg ccatcttcgg cgtttgccga aagcgaagtg
     3541 caggcgctca aaaagtttgc agaccgtggc gggaagattt tggcattcgc agaaccgacg
     3601 cgttattttc ttggtttcga ttatgtctct gggaatccga tcccgtacgg agatgtgagt
     3661 gcagtgaatt ctctgctaaa gggttgggat atttctgtta ataacgatta tctctataac
     3721 acccaaaaga atgaaggtaa cttccgtaat gtgctgtttg aagattttgg gaaaagtgaa
     3781 ctaaccttcg gcttgaacga agtggcgtta tatggttcgc gctcggttga aacggattca
     3841 gggttgattc tgcttcaagg gtcagagacc aatctctcgt cgatggatga tgcgcatcat
     3901 ccacacacgg gcggagctgc attgagcgaa gatggaagtg tagccgtctt tggcgacttt
     3961 actttcatgg catctccata taatatgtat accgataacg cgactctcat tcaaaacatg
     4021 gcagactttg cgcttgcagg tcagcaacaa caaagtttag atgtcttccc ctatctcttc
     4081 aagcaaacga ccgtgaaggt gtatgtctca tctgagatcg aaaagacttc tagcatggtt
     4141 tcagctttgg gaagtttgca atcaacaatg gcggctttga actacaaact tgaatttgta
     4201 gattcggtcc cgacctctgg tgatgccatt ttggttggaa cattcgatgc gcccgatgag
     4261 ttcgatacct acttgaagaa gtcggatgtt gaaattgacg ccgaccttct cagcaccgtt
     4321 gcatttggag aattaagcag ttctggaaat ggtttgctgc tgttcaactc caacaagagc
     4381 ggaaatacgc ttgttctttt ggctggctcg tcagacgatg tcatttcttt attaagtgtt
     4441 atgggctatg gcggcttaag ttcgtgcctt accagtgaac aagtagccgt atgcagtgtt
     4501 ggcagcggtg attattatta tgacgatacg agttctgatg tatctaccga tggaacgaca
     4561 acagatagtt ccactgaacc cactccagaa gcaacatcaa ctccagcagg gtagaggtat
     4621 ctatgagcga attaattcaa ctaggtgaag taaaaggaca ttttgcaaaa ccagaaggtg
     4681 aaggtccctt cccagcattg atcgtcattc aagaatggtg gggcttagat gcccaaacca
     4741 aatcgattgc agaccgcttt gccaaagaag ggtatctcgc gtttgcgcca gacctttatc
     4801 atggcgaact tgccca
//
LOCUS       DAOQNJ010000140         4815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10773_length_4815_cov_7.710714, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000140 DAOQNJ010000000
VERSION     DAOQNJ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10773_length_4815_cov_7.710714"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..990)
                     /locus_tag="PLG52_05485"
     CDS             complement(1..990)
                     /locus_tag="PLG52_05485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB40928.1"
                     /translation="MPQSLLNGEILRERYVVREQIGQGGTGNIYLADDVRLQGRLCAI
                     KEVEHNQVLPSDVLAQAREQFFREASVLARLDHPNLPKVSDFFSEETRDYLVMDYVPG
                     KDLRQLMQEARRSNTFIPEREVLSWANQIAEALNYLHHQDPPIVHRDIKPSNIKLTPS
                     GLVKLVDFGLVKIMAPGEVTITVIQGQGTALYTPLEQYGGDDTHTDAQADIFSFGATL
                     YHLLTNEPPAEARKRFLNSRSLTPPIDINPRISSHVEKAIFWAMALHPNDRPVDVLTF
                     RDALFGKIEIPIQVDVERYGFETPRFSVFSTEQIAGYVAFGLFLVGLLATVIR"
     gene            1179..1835
                     /locus_tag="PLG52_05490"
     CDS             1179..1835
                     /locus_tag="PLG52_05490"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB40929.1"
                     /translation="MEREEEYPILVAQDGPLKGQRWTLSKTLMMGRDPSCDIQVQDRQ
                     VSRFHARITPTQEGVTLEDLGSKNGTNHNGTEIASPIMLQDGDSFGIALAQQFTFLTS
                     DATMPLAESGPRSGRLVMEQKSRQVWVNQQQLIPPLSAQQFKLLWTLYENQGQVIERT
                     SLVSDVWGEEQTAGVSDQALDALIRRLRDRLAVLDPSHQYINTVRGHGLRLDNPPIGE
                     "
     assembly_gap    1843..1942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1998..2471
                     /locus_tag="PLG52_05495"
     CDS             1998..2471
                     /locus_tag="PLG52_05495"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNB40930.1"
                     /translation="MDAQSEKVLAQVIREARIASLGTLHDEAPQVSMVAYVYAEDFSA
                     FYIFVSRLAQHTVDMQKDNRVSLMICETDDARSDPQTLVRVSIRGKAEMLQTGEPGYT
                     PLKEQYIARFPESEKLFNLSDFSFWRISPRGGRYVAGFAKAFNITADTLQKAALR"
     gene            2523..3332
                     /locus_tag="PLG52_05500"
     CDS             2523..3332
                     /locus_tag="PLG52_05500"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNB40931.1"
                     /translation="MTQLKLLAIFAHPDDEAMGMGGTLAKYAAEGVETHLVCASRGEV
                     GWFGPEEQNPGPERLGQLRTEELQNAVNTLGMKSLSFLNYIDGQVDQAEPTEAIAKIV
                     THIRRIQPQVVVTFPHDGNYGHPDHIAIGQFTNAAIVCAADSSYKDTENLPAYRVSKL
                     YYMVDGESFINLIAPFMGDMTFPVDDQLRGENAWKEWMITTKIDMAEHCHTAWKAIMC
                     HKSQLATLGALAEMHEDAAVAVLAMQGTFFRAFSLVNGGRKLENDFFEGLR"
     gene            complement(3373..4725)
                     /locus_tag="PLG52_05505"
     CDS             complement(3373..4725)
                     /locus_tag="PLG52_05505"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HNB40932.1"
                     /translation="MAQTQIACPRCRQLIAANVEQLFDVTADPQAKQRLLSGQVNHAR
                     CPHCGYQGRLATPVVYHDNEKELLLTFFPAELGLPVNEQERMIGPLIKQVTDRLPPEK
                     RKAYLLKPQANLTYESMLETILGKDGITPEMLKEQQDRVQFIEKLMQVTSKDVRTELI
                     KQNVKIIDEQFFALFSRIAQSAMNSGQEQMARVLIDIQTQLLDETEYGRQLKESVGEL
                     EAAQHELQDAGQGLTREKLLDFVLASKSDARIRAYVSLARGGMDYTFFQLLSERIDKS
                     ADDEKTRLEGIREKLLGFVADMDKQMEARLKQAQDFVEALLAAPDIEQAVTANLDKFT
                     QDSVEVAQQMLRQATEKNEYERMGKLQKMIQVLQAASAPPEISIIEQLIQLPDAATIE
                     QALTENPEIVTQQLLDTLTGLATQMESQADNPEAKAMGEKLSEIYKIALKLSMKKNIG
                     "
BASE COUNT         1142 a         1146 c         1217 g         1210 t
ORIGIN      
        1 ctaccgaatg acggtggcaa gtaaccccac aagaaataga ccgaaggcta cataccccgc
       61 gatctgctct gtagaaaaaa cggagaagcg cggggtttcg aatccgtagc gttctacatc
      121 gacctgaatc ggaatctcga tctttccaaa caacgcatcg cggaaggtga gcacatcaac
      181 agggcggtca ttggggtgta atgccattgc ccagaagatg gctttttcca catgcgagct
      241 gatgcgtgga ttaatgtcaa tgggtggagt caggctgcgg ctatttaaga agcgtttgcg
      301 cgcctcagcg ggcggctcat tcgtcaaaag atgatagaga gtcgcgccaa aggagaaaat
      361 atccgcttgt gcatcggtgt gcgtatcgtc tccgccatat tgttcgagcg gcgtgtagag
      421 tgcagtgcct tgcccttgaa tgacggtgat ggtcacttcg ccaggcgcca tgatcttaac
      481 caaaccaaaa tcgacgagtt taaccaagcc gctaggagtt agtttgatat tgctaggctt
      541 gatatcacga tgcacgatcg gcgggtcttg atgatgcaag tagttgagcg cctcagcgat
      601 ctgatttgcc caactgagca cttctcgttc tgggatgaag gtattgcttc ggcgtgcttc
      661 ctgcattagt tgacgcaggt ctttgccggg gacgtaatcc atcacaagat agtcacgagt
      721 ttcttcggag aagaagtctg aaactttggg gaggttgggg tggtcgaggc gtgcgagaac
      781 agaagcctcg cgaaagaatt gttcgcgggc ttgtgcaagc acgtcggatg gaagaacctg
      841 gttgtgttct acttctttga tggcacacag acgcccttgt agacgaacat cgtctgcaag
      901 gtaaatattg cccgtgccgc cttgcccgat ctgttcacgg acaacgtagc gttcgcgcag
      961 gatctccccg ttcaataatg attggggcaa aacttctcct tcttggttga aagcggctgc
     1021 aagagttgag tgatattttc ggcgtgtatc acttttgcaa tcccgaaaag gggatttgca
     1081 atatataatc gtgcacagaa accacttttc tgctatatta taaacgcctt tgcaatggat
     1141 agcacaatta aatctataac aaaggcgatg aggagattat ggaacgcgaa gaggaatatc
     1201 ccattcttgt tgcacaagat ggtcccttga aggggcagcg ttggacgtta agcaagactt
     1261 taatgatggg gcgtgaccca agttgtgaca ttcaggtgca agaccgtcaa gtttctcgct
     1321 ttcacgcccg cattacaccc acgcaagaag gggtgacgct cgaagacctt ggcagcaaga
     1381 acggaacgaa tcacaatgga accgagattg ctagtccgat catgctacag gatggcgatt
     1441 cattcggaat tgcactggca caacaattca cgtttctgac ttcagatgcc accatgccac
     1501 tcgccgaaag cggaccgcgc tctgggcgct tggtgatgga gcagaagtca cgtcaggtgt
     1561 gggtcaatca acaacagttg atcccgccgc tttctgcgca acaatttaaa ctgttgtgga
     1621 ccctgtatga aaatcaaggt caggtgatcg aacgcactag cctggtttct gatgtttggg
     1681 gtgaagagca aactgcaggc gtttcagacc aggcgttgga tgcattgatc cgccgcttgc
     1741 gcgaccggct tgccgtgctc gaccccagcc atcaatacat caacactgtc cgcggacatg
     1801 gtctgcggtt agataaccca ccgatcggcg aataaattta cannnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nngtccgcgg acatggtctg cggttagata acccaccgat
     1981 cggcgaataa atttacaatg gacgcacaat cagaaaaagt actcgctcaa gttatacgtg
     2041 aagctcgcat tgcttcgctt ggcaccttgc acgacgaagc cccgcaagtc tctatggtcg
     2101 cgtatgtgta tgctgaagat ttttcggcat tctatatttt cgtcagccgc ttggcgcaac
     2161 ataccgtgga tatgcaaaaa gataatcgcg tgagtctcat gatctgcgaa acagacgatg
     2221 ctcgcagcga cccacaaacc ttggtgcgcg tgtccattcg tggcaaggca gagatgcttc
     2281 aaaccggtga gccgggctac actccgctca aagaacagta cattgcgcgt ttccctgagt
     2341 ccgaaaaatt attcaatctt tctgatttta gcttttggcg catctctccg cgtggcggac
     2401 gctatgtggc aggttttgcc aaagcattca acatcacagc cgacaccctg caaaaagcag
     2461 cccttcgata acctcattga cagtcactcc ggtggctgtc agttttttta ggagcaatca
     2521 atatgacaca acttaaactg ctggcgatct ttgcgcaccc cgatgatgaa gctatgggca
     2581 tgggcgggac gttggcgaag tatgccgccg aaggcgtgga gactcatctc gtctgcgctt
     2641 cacgcggaga ggtgggctgg ttcggccccg aagaacagaa tccaggtccc gagcgactcg
     2701 ggcaactccg caccgaagag cttcagaacg ccgtcaacac attaggcatg aaaagccttt
     2761 cttttttgaa ttacatcgat ggtcaagtgg accaagccga gccaactgaa gcaattgcga
     2821 agatcgttac acatattcgt cgcatccaac cgcaagtggt ggtgactttc ccgcatgatg
     2881 gtaattacgg tcatcccgac cacatcgcta tcggacagtt caccaatgct gccatcgtct
     2941 gcgccgcgga ctcaagctac aaagacactg agaatcttcc tgcttaccgt gtctctaaac
     3001 tttattacat ggtagacggt gaaagtttca ttaacttgat cgcacccttt atgggcgata
     3061 tgaccttccc ggttgatgac caactccgtg gcgaaaatgc ttggaaagaa tggatgatca
     3121 ccactaagat cgatatggca gaacattgtc acaccgcatg gaaggcgatc atgtgccaca
     3181 aaagccaatt ggctaccttg ggcgctctcg ccgaaatgca tgaagatgca gccgtggcag
     3241 tccttgccat gcagggaact ttcttccgtg cctttagttt ggtgaatggg ggtagaaagt
     3301 tagagaacga tttctttgaa ggattgagat aataaaaaag aaggtcacgt tgtaatagcg
     3361 tgaccttctt ttttatccta tatttttctt catcgagagc ttgagcgcaa tcttgtaaat
     3421 ttcactcagc ttctctccca tggcttttgc ttcggggtta tcggcttgtg attccatttg
     3481 cgtggcgaga cccgtcagag tatcaagcaa ttgttgcgta acgatctctg ggttttccgt
     3541 cagcgcttgt tcaatggtgg cggcatcggg caactggatc aactgctcaa tgatggaaat
     3601 ctcaggcggg gcgctggcag cttgtagcac ctgaatcatc ttttgcaatt tgcccatgcg
     3661 ttcgtattca tttttctcag tagcttgtct gagcatttgc tgggcaactt ctactgaatc
     3721 ttgcgtgaac ttatcgaggt tggcagtgac ggcttgttca atatctgggg cagccaacag
     3781 agcctcaaca aagtcttggg cttgtttgag gcgcgcttcc atttgcttgt ccatatcggc
     3841 tacgaaccca agcaacttct cacggattcc ttcgaggcgg gtcttttcgt catctgccga
     3901 tttgtcgata cgttcgctca aaagctggaa gaaggtgtaa tccatgccgc cacgagccag
     3961 cgaaacatag gcgcggatgc gagcgtcaga cttggatgcc agaacgaagt cgagcaattt
     4021 ctcgcgcgtc aacccctgac ccgcgtcctg caactcgtgt tgagccgctt ccaactctcc
     4081 aactgattct tttagctgcc gtccatattc ggtttcatcc agcaattgag tttgaatatc
     4141 aatgagcaca cgtgccattt gttcttgtcc gctattcata gcgctttgcg cgatgcggct
     4201 gaacaaggcg aagaattgtt catcgataat cttgacattc tgcttgatca actctgtgcg
     4261 gacatctttc gacgtgacct gcatcaactt ctcaatgaac tggacgcggt cttgctgttc
     4321 cttcaacatt tcaggcgtaa tgccatcttt gccgagaatg gtttctagca tcgactcata
     4381 cgtcaggttt gcctgaggtt tgagaaggta ggctttgcgc ttctcaggag ggaggcggtc
     4441 agtcacctgc ttgatgagtg gaccgatcat ccgttcctgt tcattgacgg gcaaccccaa
     4501 ttcagcgggg aagaaggtga gcaaaagctc tttttcattg tcgtgataca ccaccggtgt
     4561 ggcgaggcgt ccttggtagc cgcaatgtgg gcaacgggcg tggttaactt gtccgctcaa
     4621 caagcgttgt ttggcttgtg ggtcagcagt gacgtcgaat aactgctcta cattggcagc
     4681 gattaattgt ctacaacggg ggcatgcaat ttgtgtttga gccattacga tttccgattt
     4741 gacgatttgg gattgggtaa tacagaaccc attttacacg aattaatctt gagagacata
     4801 aattacgcac cacgt
//
LOCUS       DAOQNJ010000141         4806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10809_length_4806_cov_8.218901, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000141 DAOQNJ010000000
VERSION     DAOQNJ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10809_length_4806_cov_8.218901"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLG52_05510"
     CDS             <1..641
                     /locus_tag="PLG52_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40933.1"
                     /translation="TLLPESKYEGDKVIALWHIGKHSFVQLDPAYYSQLEDLIKKLPH
                     HTSLTGEQIEAAWGKDAISSRDVGFMHYLFPSDLPHYQPSLPFTLRQLTEADAGLMST
                     LQAVNSPEDVDEAFVEVTHEIAFGCFHGEELVAAASGYERTGFLDLGVLTHPDYRKKG
                     LGKAVVGATCNWANQKGIIAQYRHNALNTSSQNVATSLNFQKYFSSESFFLR"
     gene            646..1920
                     /locus_tag="PLG52_05515"
     CDS             646..1920
                     /locus_tag="PLG52_05515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40934.1"
                     /translation="MAYLKTLYNQYPRQFWLMILGIVISTAGGGMVWPFMLIYAGGKL
                     GLPLSAVASLISINAGVGLFASFLAGTLADRIGRKVVMVSSLVMMGVAYLLLIKAETY
                     LQFALLMGMVGLSNPLYQVGADAMLADMIPTQKRTDAYAINRIAYNASFGIGPAIGGF
                     LASTSYALAFYGASTGFFIYGIIWFFFAHETLDKIVREEKASTLAGQAQIQNEAARSD
                     GYIHVFKDKAYMAFVTLFAIGMIAPSILWILLPVYTKTNFNLPEALYGWIPTTNAFMC
                     VFLQYAVSNQTRKFKTLPVASVGMLVYALGAGSVALMTGFTGFWLSMVILTFGELILI
                     PIASKYVADIAPENLRGRYMSLYWFGWGMSRTLAPLIGGYLNDNISPRAIWIGGLLFG
                     LTSTLGLILLNSLTNTQTALQTRPKIDTTHGD"
     assembly_gap    2026..2125
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2170..2769)
                     /locus_tag="PLG52_05520"
     CDS             complement(2170..2769)
                     /locus_tag="PLG52_05520"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNB40935.1"
                     /translation="MTNWAQRVTLQGKYVRLEPMGEQHVSELAEIGAGQDFWSFMLYG
                     DMKTEADMRNWVLDILEREKKGGDLPFVVIHLASGKVAGATRYLNIIPKDRGLEVGGT
                     WYGKEFQRTAVNTECKYLLLTHAFEVLKTIRVQIKTDSINLRSQAAIERIGAVKEGVL
                     RNHMILPDGRIRHSVFYSILDSEWVGVKKNLEAMLKKNE"
     gene            complement(2766..3185)
                     /locus_tag="PLG52_05525"
     CDS             complement(2766..3185)
                     /locus_tag="PLG52_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB40936.1"
                     /translation="MKDIWEFFCNPYNLNTITPPDMNFKIIKGGDRKMHPGQMIEYQV
                     EFIRGIRSLWLTEITHVREGEFFVDEQRIGPYRLWHHEHIFESVNGGVKMTDRVTYML
                     PFELLGDIVHAVWVRRRLDSIFDFRTQKTTELFGYLK"
     gene            complement(3224..>4806)
                     /locus_tag="PLG52_05530"
     CDS             complement(3224..>4806)
                     /locus_tag="PLG52_05530"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNB40937.1"
                     /translation="LATMFLKDLFVDFPFSRPSNIPDVEINEIVIDSRAVKPGNLFVA
                     MRGRDADGHTYIQKAIDNGAVAVIGDKELSGFSVPYIQLENARHSLTWLAAAFHGFPA
                     RKLTVVGVTGTDGKTTTSNLLYKILIAAGLKAGMISTVNAVIGDEVLDTGFHVTTPDA
                     HDVQRYLAKMVSAGLTHVVLETTSHGWSQHRIDACEFDIAVVTNITHEHMDEHGGYEN
                     YRAAKARLFESLEWTRAKPQGNPRLGVINFDDKKSFDFLNDFIKTKKINYGLGEGSDV
                     RGMNIAYSPSGIHFTAVAKDIRVAVSSNLVGAYNVSNCLAALTAAVYGLGISPEIVAE
                     GIASLEGIPGRMERIDMGQNFTAIVDFAHTPNALKVALEAGRTMTDKRVIAVFGSAGL
                     RDKEKRRMMAETSAELADLTVLTAEDPRIESLNGILEEMANGMKSKGGREGVNFWRVP
                     DRGEAIKFALTLAQPGDIVLSCGKGHEQSMCFGKTEYLWDDRTAMRAALADYLEVQGP
                     AMPYLPSQDTPEEEWLKG"
BASE COUNT         1223 a         1214 c         1008 g         1261 t
ORIGIN      
        1 ctacactttt gccggaaagc aaatatgagg gcgataaagt cattgccctt tggcacatcg
       61 gaaaacacag cttcgtgcaa cttgacccag catattattc tcaactggaa gatttaatca
      121 aaaagctgcc acaccatact tcattgactg gtgaacaaat cgaagcagca tggggtaaag
      181 atgccatatc ttcacgagat gttggcttca tgcattatct ctttccatca gacttacccc
      241 attaccaacc ctcattgcct tttacactgc gccaactgac cgaagcggac gcgggtctta
      301 tgtctacttt acaggcggtg aactcacccg aagatgtcga tgaagccttt gtggaagtga
      361 ctcacgaaat tgcattcggc tgttttcacg gcgaagaact tgttgctgct gccagcggct
      421 acgagcgaac aggctttctc gacctcggcg tgctgacaca ccctgattac cgtaaaaaag
      481 ggctaggcaa agctgtggtt ggtgcaactt gcaattgggc aaaccaaaaa gggataatcg
      541 ctcaatatcg tcacaacgcg cttaatacca gttcacaaaa tgtagctacg tctttgaact
      601 ttcaaaaata ctttagttca gaaagctttt ttctcaggta acttcgtggc atacctcaaa
      661 actttatata accagtatcc acgccagttc tggttgatga tccttgggat tgtcatcagt
      721 actgcaggcg gcggaatggt ctggcccttc atgcttatct acgccggagg caaactgggt
      781 cttccgctca gtgcggtggc gtcgctaatc tctattaatg caggtgtagg tttattcgca
      841 tcttttctcg ccggaacttt agcagaccgc attgggcgta aagtggtgat ggtctccagc
      901 ctcgttatga tgggtgtcgc ctatcttctc ctcatcaaag cagagactta ccttcaattt
      961 gcattgctaa tgggcatggt tgggttgtca aacccgcttt atcaagtggg tgccgatgcc
     1021 atgttggcag acatgattcc cactcaaaag cgaaccgacg cctatgccat caaccgtatc
     1081 gcctacaatg cctcgtttgg gattggtcca gcgattggtg ggtttcttgc gtccacttcg
     1141 tatgcgctcg cattttacgg ggcatctaca ggattcttta tctatgggat tatctggttt
     1201 ttcttcgcgc acgaaacgct tgataaaatt gtccgcgaag aaaaagcttc cactttagcc
     1261 gggcaggctc agatccagaa tgaagcggcg cgatccgatg ggtacatcca cgttttcaaa
     1321 gacaaggcat acatggcgtt cgtcacttta tttgcaatcg gtatgatcgc gccttctatc
     1381 ttatggattc ttctaccggt atacaccaaa accaacttca atttacccga agcgctatat
     1441 ggttggattc caaccacaaa cgcattcatg tgcgtgttct tgcaatatgc cgtttcgaac
     1501 caaacgcgta aattcaaaac cctaccggtt gcaagcgttg gcatgcttgt gtatgcgctt
     1561 ggtgccggca gtgtggcgct catgacgggc tttacagggt tttggctaag catggtcatt
     1621 ctcacgtttg gcgagctaat tctgattcca atcgcaagca aatatgtggc agatatcgca
     1681 ccagaaaatt tacgcggaag atatatgtca ctttattggt ttggctgggg catgtcgcgc
     1741 acacttgcgc cgctaatcgg tggctatctg aatgacaaca tctcaccacg cgcaatttgg
     1801 attggaggtc tgctcttcgg cttgaccagc accctcggct tgattctact caacagcctg
     1861 accaacaccc aaacagcgct tcaaactcga cccaaaattg atactaccca tggagattaa
     1921 ctatgaagca aactcttgca ctgatttctt acgttgtccg tgattatgat gaagcgattg
     1981 cgtttttcac caatgctatg caatttgatt tggtcgaaga tacgcnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnntatgg aaacaagtgg gatttgctac aaccaaaata
     2161 aaaatattgc tattcgtttt ttttcaacat ggcttccagg tttttcttta ccccaaccca
     2221 ttccgaatcc aagatcgaat agaatactga atgccgaatg cgcccatccg gcaaaatcat
     2281 gtgattacgc aagactcctt ctttcaccgc gccaatgcgt tcaatggcag cctgagaacg
     2341 gaggttgatc gagtctgttt tgatctgtac acgaatggtt ttaagaactt caaaagcatg
     2401 ggttagcaag aggtatttgc attctgtatt cacagccgta cgttggaatt cttttccata
     2461 ccaggtacca cctacttcaa gtccgcgatc tttgggtatg atattcaggt aacgagttgc
     2521 acctgccacc ttgccagatg caagatgaat aacgacaaaa gggagatcac cacctttttt
     2581 ctcgcgctcc agaatatcca atacccaatt tcgcatatcg gcttcggtct tcatatctcc
     2641 atacaacatg aagctccaaa aatcctgccc agcaccgatc tctgccaact ccgatacatg
     2701 ttgttcgccc atcggttcga gacgcacata tttcccttga agcgtgacac gctgagccca
     2761 atttgtcatt tcaggtaccc aaacaactcg gtagtctttt gggtgcgaaa gtcaaagatg
     2821 gaatccaacc gtcttctcac ccaaaccgca tggacaatat ctcccaacaa ctcaaacggc
     2881 aacatgtacg tcacacggtc ggtcatctta acgccaccat tcacactctc aaagatgtgt
     2941 tcatgatgcc acaaccgata tggacctatt cgttgttcat ccacaaaaaa ctcaccttcg
     3001 cgaacatgcg ttatctccgt cagccataac gaacgaatgc cacggataaa ctccacctga
     3061 tattcgatca tctgcccggg gtgcattttt ctatctccgc ccttaataat cttaaagttc
     3121 atatcaggcg gagtaatcgt attcagattg tatgggttac aaaaaaattc ccagatatct
     3181 ttcatggttc cgggaattac ttgctctcgt tctaaataat gaatcatccc ttcaaccatt
     3241 cttcttctgg tgtatcttgc gaaggaagat aaggcatggc aggtccctgc acctcaagat
     3301 aatccgcaag agcggcacgc atggcggtgc ggtcatccca taaatactcg gtcttgccaa
     3361 agcacatcga ctgttcatgc ccctttccac aggaaaggac aatatcacct ggttgagcaa
     3421 gtgtcagcgc aaacttgata gcttcgccac gatctggcac acgccagaag ttcacacctt
     3481 cgcgtccgcc cttggacttc ataccgtttg ccatttcttc aaggattcca ttcagcgact
     3541 caatgcgtgg gtcttcagca gttagcaccg ttaagtcagc taactctgca gaagtctctg
     3601 ccatcatacg gcgcttttcc ttatcacgaa gccctgccga accaaagaca gcgatgacac
     3661 gtttatctgt catcgttctg ccggcttcta atgcaacttt caatgcatta ggtgtgtgag
     3721 caaaatccac aatggcagtg aagttctgcc ccatatcaat gcgctccata cggcctggaa
     3781 tcccttctag agaagcaata ccctcagcaa ctatctctgg agaaatgcca agcccataca
     3841 cagccgcagt caacgcggcg agacaatttg agacgttata tgccccaacc aaattacttg
     3901 ataccgctac acgaatatct ttggctaccg ccgtaaagtg gattccagat gggctatatg
     3961 cgatattcat cccgcgaaca tcgctccctt cgcccagccc ataattgatt ttctttgtct
     4021 tgataaagtc gttcagaaaa tcaaaggact ttttatcgtc gaagttgatc acacccaaac
     4081 gcggattccc ttgcggcttg gcgcgtgtcc attcgaggct ttcaaataat ctcgccttgg
     4141 cagcgcgata gttttcatag ccgccatgtt cgtccatatg ttcgtgtgta atgttggtca
     4201 caaccgcaat atcaaattcg caggcgtcga tacgatgttg agaccaacca tgagacgtgg
     4261 tttccagcac aacatgggtc aagccggcag ataccatttt tgccaaataa cgttgcacat
     4321 cgtgggcgtc tggtgtggtg acgtggaaac cagtatccaa cacctcgtca ccgatcacgg
     4381 cattcaccgt agagatcatg cctgctttga gtccagccgc gatgaggatt ttgtaaagca
     4441 gattactggt tgtcgttttg ccatccgtgc cggtcacacc gacaacagtc agcttacggg
     4501 caggaaagcc atgaaacgcc gcagcaagcc aggtcaagga atgacgcgcg ttctccaact
     4561 gaatgtacgg aacgctaaat ccgctcagtt ctttatcccc aataaccgca acagcgccat
     4621 tatctattgc tttttgaata taggtatgcc catctgcatc acgtccgcgc atggcgacaa
     4681 atagattacc cggctttaca gcccggctat caatgactat ttcattgatt tcaacatcag
     4741 ggatgttaga aggacgagaa aagggaaagt caacgaacaa gtctttgaga aacatagttg
     4801 ctagtt
//
LOCUS       DAOQNJ010000142         4804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_10816_length_4804_cov_9.266793, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000142 DAOQNJ010000000
VERSION     DAOQNJ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10816_length_4804_cov_9.266793"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1177)
                     /gene="wecB"
                     /locus_tag="PLG52_05535"
     CDS             complement(65..1177)
                     /gene="wecB"
                     /locus_tag="PLG52_05535"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNB40938.1"
                     /translation="MRILTVFGTRPEAVKMAPIVKLLASTPGVEARVCVTAQHRQMLD
                     QVLNLFQIEPHYDLNLMRDGQSLAQLSADIFTHLDPVLEDFKPDWVLGQGDTTTVAIT
                     SMLCFYRQIRFGHVEAGLRTHDKWQPFPEEVNRRIVSVIADLHFAPTNWSKNNLLREG
                     VDEKTVRVTGNTVIDALLFVSKQAEPAEITELVQSKLSHKKLILVTAHRRENFGAPME
                     NICKALLQIASRGDVEIVYPVHLNPNVQEPVSRLLRGVEHVTLLPPLDYLPLVHLMKH
                     ANIILTDSGGIQEEAPAFGIPTLVLRDVTERPEGVDAGTLKLVGTETSHIVEETRRLL
                     EDESAYKKMATSANPYGDGHAAEKIIQALLGSRENM"
     gene            1226..1381
                     /locus_tag="PLG52_05540"
     CDS             1226..1381
                     /locus_tag="PLG52_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40939.1"
                     /translation="MFKLIKFTKRSIILALYFSLILGLASCAKATCGMQAVAGGSQIC
                     IEVLNAE"
     gene            complement(1382..2692)
                     /locus_tag="PLG52_05545"
     CDS             complement(1382..2692)
                     /locus_tag="PLG52_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNB40940.1"
                     /translation="MKYIKICVIGLGYIGLPTASTFAMNGIEVLGVDISPTIIETLNR
                     GEIHIHEPGLSEAFTNAVKSGKFKAALKPEEADAFIIAVPTPFKHNETGNYNGMTYKL
                     ADMEAVTSAAESIVPYLRKGNVVVLESTSPPRTTFDLVAPILEKSKLKAGVDFHLCYS
                     PERVLPGQILRELIENSRVIGGVTPESAKAGADLYSTFVKGQIIQTDATTAEMVKLME
                     NTYRDVNIAIANEFSRLADKFGVDVWEAISIANLHPRVKILSPGPGVGGHCISVDPWF
                     FVEAAPNLTPLIYNSRQVNDAQPAFVVDVVKRALGSLKGKKIAALGLAYKPDVDDLRE
                     SPAVEAVHFFQKEGAEVLACEPFKPDAKLPGVTIAPTFEDAVRDADAIILLVKHTEFS
                     KLKPEQVAQQTKARLLIDTVQGWDAEQWKHAGFRHVKLGSGKPA"
     gene            2822..3418
                     /locus_tag="PLG52_05550"
     CDS             2822..3418
                     /locus_tag="PLG52_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNB40941.1"
                     /translation="MDFLLDPNIAYLFLLGGVVLAFLAIVTPGTGMLEVGAIFCFLLA
                     GYAVYNLPMNWWALLILLLSVVPFLYAVQKPKRELFLGVSIFLLVLGSVFLFAVDGWK
                     PAVNPLVALFSSGVVAAFLWIVVRKSIQAADARPTHDLGILVGQMGEARTAVHQEGSV
                     YINGELWSAKSVSEIPAGSHVLVVAREGFVLVVEPIKS"
     gene            3436..4344
                     /locus_tag="PLG52_05555"
     CDS             3436..4344
                     /locus_tag="PLG52_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNB40942.1"
                     /translation="MNGAFASFGLCLVGFIVLVIVIAAFNMIRVVPEYQRLVVFRLGR
                     VLEKPKGPGLVFLIPGVDRPVKVDLREQVREVPHQTSITKDNAPISIDFLWYYKVLDP
                     AQSVLQVGNFESAAAGIAATTLRAVIGGIPLDDVLSEREHINNMLRTRLDEVTERWGV
                     KVTNVEIREIIPPRDVQDAMNRQMSAERIRRAVVTESTGTREAAVNVAEGEKQSNILR
                     AEGQKQSAILQAEGERQAQLLRAQGFAQALETIFGAANKIDQKTMTLQYFETLKSMGM
                     SPSTKYIFPMEFTSMLDNFLGKDSKK"
     gene            4395..>4804
                     /locus_tag="PLG52_05560"
     CDS             4395..>4804
                     /locus_tag="PLG52_05560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40943.1"
                     /translation="MEIIPANILDLTALRKLEKESFDRDAWPMIDLIAVLTFSNVVRL
                     KAMENGNMIGFVAGDPRPRDGWGWIATIAVDSNFRRRGIGTALLHACESQLGVPRSRL
                     TVRTSNQGAIILYEKEGYRTIDVWKAYYSDGEDA"
BASE COUNT         1161 a         1062 c         1208 g         1373 t
ORIGIN      
        1 ccaccactgt cattatactc agaaacaccc ctcaagacat tacaaacagt tatcttgaga
       61 aagattacat gttttcccta ctgcctagta aagcttggat gattttctct gcagcgtgtc
      121 catctccgta tggattggcg gatgttgcca tctttttgta tgcagattca tcctctaaga
      181 gccgcctcgt ttcttcgacg atgtggctgg tttcggttcc aacgagcttt aatgtccccg
      241 catcgactcc ctcagggcgt tcggtcacat cgcgtagtac cagtgttggg attccaaagg
      301 ctggggcttc ttcctgaatc ccgcctgaat cggtcaatat gatgtttgca tgcttcatca
      361 aatgaacgag cggaagataa tccagtggcg gtagcagagt aacatgctcc acgcctctca
      421 atagacggct aacaggttct tggacatttg ggttgagatg aacgggatac acgatctcta
      481 catcccctcg gcttgcgatt tggagcaatg ctttacaaat attctccatc ggcgcgccaa
      541 agttctcacg gcgatgcgcc gtgacgagaa ttaacttttt atggctcagt ttagattgca
      601 ccagttcggt gatttctgct ggttcggctt gctttgacac gaacagcaaa gcatcaatga
      661 cagtgttccc ggttactcta acggttttct cgtccacacc ttcgcgcaat aaattgttct
      721 ttgaccaatt cgtgggcgca aaatgcaaat ccgcgatgac gctgacaatg cggcggttta
      781 cttcctctgg gaaaggctgc cacttgtcat gggtccgcaa accggcttcc acatgcccga
      841 agcgaatttg gcgataaaag cacaacatgg atgtgatggc gacggtggtg gtatcgcctt
      901 gtccaagcac ccagtctggt ttgaaatctt ctagaacagg gtcgagatgc gtgaaaatat
      961 ccgcacttaa ttgggcaaga gattgtccat ctcgcataag gttcaaatca taatgtggtt
     1021 caatttggaa caggttcaac acttggtcaa gcatctggcg gtgttgcgcc gtaacacaaa
     1081 cccgagcctc aacacctggc gtagaagcaa gcagcttcac aattggtgcc attttgacgg
     1141 cttcgggtcg agttccaaaa actgtaagga ttctcataag tcctttttgg gcctgttttt
     1201 ctactaggtg tgtggagaaa caaatatgtt caaattaatt aagtttacaa aacgttctat
     1261 aattttggcg ctttatttca gccttattct agggcttgca tcttgtgcaa aagccacctg
     1321 cggtatgcaa gcggttgcag gtggctcgca aatatgtata gaagttctga acgctgagta
     1381 actatgcggg ctttccagac ccaagcttga catggcggaa ccctgcatgc ttccattgct
     1441 ctgcatccca accttggacg gtatcaatca agaggcgtgc ttttgtttgt tgagcaactt
     1501 gttcaggctt gagcttgcta aattcggtat gttttacgag caagataatc gcgtcggcat
     1561 cacgaactgc atcttcaaag gttggcgcaa ttgttacgcc gggcaatttg gcatctggtt
     1621 tgaaaggctc gcaagcgaga acttctgcgc cttctttttg gaagaagtgc accgcttcaa
     1681 cagccgggct ttcacgtaaa tcatccacat caggcttgta tgccaagccg agagcggcga
     1741 tctttttccc tttgagggaa ccaagtgcgc gtttgaccac atctacaaca aaggctggtt
     1801 gcgcatcgtt gacttggcga gagttgtaaa tcagcggagt gagatttggc gccgcctcta
     1861 caaagaacca agggtctacg ctgatacagt gaccaccaac ccctggtccc gggctgagaa
     1921 ttttgacacg tgggtgcaaa ttggcaatgg agatggcttc ccagacatcc acaccgaatt
     1981 tatcggcaag gcgagagaat tcattagcaa tggcaatgtt gacatcgcga taagtattct
     2041 ccatcagttt gaccatttcg gctgtggtcg catcggtttg aatgatctgc cctttgacga
     2101 aggtagagta caagtctgct ccggctttag cagattcagg ggtcacgccg ccgataacgc
     2161 gtgagttttc aatcaactcg cgcagaattt gccccggcaa gacgcgttct ggtgaatagc
     2221 aaaggtgaaa gtccacgccc gctttcagct tggatttttc taaaatgggg gcaaccaagt
     2281 caaaggtcgt gcgaggcgga gaagtcgatt cgaggacgac cacatttccc ttcctcaaat
     2341 atggcacaat ggattccgct gcagaagtca cagcttccat atccgccagt ttgtaggtca
     2401 tgccgttgta gttgcctgtt tcattatgtt tgaatggcgt tggaaccgca atgataaaag
     2461 catcggcttc ttctggtttg agagccgctt tgaattttcc gcttttgaca gcgtttgtaa
     2521 atgcttctga aagacccggt tcgtgaatgt ggatctcgcc gcggttcaag gtttcaataa
     2581 tggtggggct aatgtctaca ccgagaactt cgatgccatt cattgcgaat gtagatgctg
     2641 tgggtagacc aatgtatccc aacccgataa cacatatttt tatgtacttc acttgagctc
     2701 ctatgggagt aattatcctg tccattatac aactgctaga gaaattggtt tgaaacgttt
     2761 caatgggcat tacgtaaatc tttataggaa tgctgttgta taattatatg gaggcataaa
     2821 catggatttc cttttagacc ctaatattgc ctatttgttc cttcttggcg gagttgtatt
     2881 agcctttttg gctattgtta cgcctggaac cggaatgtta gaggtgggag cgattttttg
     2941 tttcttgctg gctggttatg ctgtctataa tctccctatg aattggtggg cattgctcat
     3001 tttacttttg agtgttgtgc ctttccttta tgcagttcaa aagccaaagc gagagttatt
     3061 ccttggagtt tcaattttcc tgttggtgct cggctctgtg tttttgtttg ctgtagatgg
     3121 ttggaagccg gcagtgaacc cattggttgc tttgttttcg tcaggtgttg tagctgcgtt
     3181 tctttggatt gtcgttagaa agtccattca agcggcagac gctcgaccaa cgcatgattt
     3241 gggtattttg gtgggacaga tgggggaggc aagaactgct gtacatcaag aaggaagcgt
     3301 gtatattaat ggggaattgt ggtcggcaaa gagtgttagt gagattcctg ctggaagcca
     3361 cgttctagtt gttgctcgcg aggggttcgt cctcgttgtt gaacctatca aatcgtaaat
     3421 tttgaaggag agaatatgaa tggtgcattt gcaagttttg gtttgtgttt ggtcggtttt
     3481 atcgttctag tgattgtgat tgccgcgttc aatatgattc gggttgtgcc agaatatcaa
     3541 cgtttggtcg tgttccgttt gggacgtgtg ctggagaagc ccaagggacc aggcttggtc
     3601 ttcttaatcc ccggtgtgga tcgccctgta aaagttgatt tgcgtgaaca ggttcgtgaa
     3661 gttcctcatc agacttcaat cacaaaagat aacgcgccaa tctcaattga cttcttgtgg
     3721 tattacaagg tactcgaccc tgcccagtct gtattgcagg ttggtaactt cgagtctgct
     3781 gcggctggta ttgctgccac tactttgcgt gctgtcatcg gtggtatccc attggatgat
     3841 gtgctctctg aacgtgaaca tatcaataat atgctccgca cccgtcttga tgaagtgacc
     3901 gaacgctggg gtgtgaaagt aaccaatgtt gaaatccgcg aaatcattcc tccgcgtgat
     3961 gtgcaagacg ccatgaaccg ccagatgtct gcagaacgta ttcgccgtgc tgttgtgacc
     4021 gagtctactg gtacgcgtga agctgcagtc aacgtggcag aaggtgaaaa gcagtctaac
     4081 attttgcgag cagaaggtca gaaacagtct gcaattcttc aagcagaagg tgaacgacag
     4141 gctcaattgc ttcgcgcaca gggttttgct caggcgttgg aaaccatttt tggcgctgcc
     4201 aataagatcg accagaaaac tatgaccctc caatacttcg aaaccttgaa gtcaatgggg
     4261 atgagtcctt ccacgaagta catcttcccg atggaattta ccagtatgct cgataacttc
     4321 ctcggtaaag actcgaagaa gtaaaacgat ttgtaataca ataaggtctc tcgaaaagag
     4381 agaccttatt ttttatggaa atcatccctg ccaatattct tgacctcacc gctctgcgaa
     4441 aattagagaa ggaaagtttt gaccgcgatg catggccaat gattgacctg atcgccgttt
     4501 tgacattctc caatgtggtg cgcctcaaag ctatggaaaa tggcaacatg atcggttttg
     4561 tcgctggcga tccgcgtcca cgcgacgggt ggggctggat cgccaccatt gcagtggatt
     4621 caaactttcg gcgccgcgga attggaacgg cattgttgca tgcatgtgag agccagctag
     4681 gtgttccaag gtcgcggtta acggttcgaa catcaaatca aggggcgatt atcctatatg
     4741 aaaaagaagg atatcgcacg atagatgtct ggaaagcata ttattctgat ggcgaagatg
     4801 ccat
//
LOCUS       DAOQNJ010000143         4723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11119_length_4723_cov_10.144602, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000143 DAOQNJ010000000
VERSION     DAOQNJ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11119_length_4723_cov_10.144602"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="PLG52_05565"
     CDS             <1..1342
                     /locus_tag="PLG52_05565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB40944.1"
                     /translation="MKTKSELFIRDLGDGLILRHGSVEDADALADINSRMHSDEGPDH
                     PNLGIGAWIRDLAAKPHPTLVPSDFTVVEETATGKIVSTLCLIPQTWTFDGIEFGVGR
                     PELVCTLPEFRKRGLIRVQMDEVHKWSTDRGHLAQVITGIPYFYRQFGYDMALNLSGV
                     RRGFEPHVPILKEGETEPFNIRPAKDADVDFILATYAAHESKQAISCKRTPDILRYEM
                     NGKDALNLSRYEFAVIEDSNGKRLGFFEHSIELWFGGVGCVYFALAEGTSWLGVAPSV
                     VRYLWKKGAEYTQRESTTRTTFSFNLGEQHPVYEVLDDKLPAVRKPYAYYVRVPDVPA
                     FLNQVKPALEKRLSNSVAVGYSGELRVGFYKDGFRMVFENGNITLIEALKHTTDSDVD
                     IAFPEYSFLHVLFGHRSLDELRHAFVDCYVQNPTARVLVNALFPKRDSFVQAVS"
     gene            1344..2597
                     /locus_tag="PLG52_05570"
     CDS             1344..2597
                     /locus_tag="PLG52_05570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40945.1"
                     /translation="MFRIEASKVYYFIEFTSSAFFSMMFVVISLYEATVAGLTPMQLV
                     LVGTTLEVSAFLFEIPTGVVADVFSRRLSIIIGYIMMGVGFLVEGLFPSFLPILLAQV
                     IWGVGYTFTSGATQAWITDEVGEEKANPLFMRAARVGLVASLIGMGATMLIGANNAAM
                     PIIVGAIGVILIGIVLIFIMPETGFKPTPMENRSTWQHMWHTFNEGVKSVKARPRLMN
                     ILGVGFFYGLYSEGFDRLWVKHLLDTFQIPVLFGGNQVAFFSLLRFSAGLLTVFVMQL
                     IEKRVDASSPLSIGRAMMIVTGVIALGIVGFALSSLLPLTLGLYLTIYILRQVRIPLQ
                     TAWVNHKLDPQTRATVHSMTGQVDSIGQILGGGSAFIARYLSVVAAITTSGILLGPAL
                     GLVRRANSQSTHEAEVDPVGELTGD"
     gene            2725..3531
                     /locus_tag="PLG52_05575"
     CDS             2725..3531
                     /locus_tag="PLG52_05575"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNB40946.1"
                     /translation="MALRGNLRDFTITQLLNLINLASKTGTLVIDGASEQAHISFREG
                     KLAYARIGKEDGSLALVLYKANKINANQYKAIIDRAGQMSDKELGLLLINAGYVSRDD
                     ILLNLQAYFTDMVRRMFTWVEGVFRFEAEMLPPDDRINVRLDLENIIIEGSRQLRELE
                     QLQDEIPSLDMALKFTDRPLTNVNLSVDEWKVVKFVDPKNTMRQIAKTNKLTEVEVRR
                     IVYGLLQAGLVELVRPADAPTPQAAKPLAPQEMEDKKNLVNKLIGRIRSL"
     gene            3559..4095
                     /locus_tag="PLG52_05580"
     CDS             3559..4095
                     /locus_tag="PLG52_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNB40947.1"
                     /translation="MQTVKMVVTGPFSAGKTQFIQSVSEIDVVATERKISSDEERSVK
                     SATTVAMDFGRITVDQDLVLYLFGTPGQKRFDFMWEILSEGMLGFIVMVDSTRPETFR
                     EARSILETFRAYAPTPFVVAANKQDKEDAWDLEDMGHALRLDKNTKLLPCVATDRNSV
                     KTILLELLYSIMSEMEGK"
     gene            4116..>4723
                     /locus_tag="PLG52_05585"
     CDS             4116..>4723
                     /locus_tag="PLG52_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB40948.1"
                     /translation="MSSITTDQGIVHYEVFGRGKPVILLHGYQGSWGLWQETMAYLGA
                     FYRTYALDFWGFGESGTKRSTYAVKDFVSLVYQFMEQLGIVRAPLVGHSMGGTVSLMT
                     AMQHPDRIQKVVVIGSPISGSSLYFFPRVFGYKTFGWLTYHNLWIYKGFYRILSPFYS
                     KDKNWAKMMDRDVSQVSLEAFFSSIGSLRRTDLRPSLHTIQAP"
BASE COUNT         1122 a         1065 c         1241 g         1295 t
ORIGIN      
        1 catgaaaaca aaatctgaac tttttatccg tgaccttggt gacggattga ttctgcgcca
       61 tggctctgta gaagatgccg acgccttggc agatattaac agccgtatgc atagcgatga
      121 aggaccggat caccccaacc tcggaattgg tgcgtggatc cgagacttgg cagccaaacc
      181 acacccaacc ctcgtgccaa gcgatttcac ggtggtggaa gaaacagcta ctggaaaaat
      241 cgtttctacg ttatgtctca tcccccaaac gtggaccttt gatggcattg aatttggcgt
      301 agggcgccca gaactggttt gcaccttgcc cgagtttcgc aaacgtggac tcatccgcgt
      361 gcaaatggac gaggtccaca agtggagcac agaccgcgga catttggcac aagtcattac
      421 gggcattccg tatttttatc gtcagttcgg gtacgacatg gcgctaaatc tctcaggggt
      481 tcgccgcggg tttgaaccgc atgtgcctat cttgaaagaa ggggagacag agccattcaa
      541 cattcgccct gcaaaagatg ccgatgtgga ttttattttg gcgacctatg cagcccatga
      601 aagcaaacaa gcaatctcgt gtaaacgtac gccagatatt cttcgttatg aaatgaacgg
      661 caaagatgcc ttgaacctca gccgttatga atttgcagtc atcgaagatt caaacggcaa
      721 acgccttggc ttctttgaac attccattga attatggttt ggtggtgtgg ggtgtgtcta
      781 ctttgcattg gcagagggca catcttggtt gggggttgcg ccaagcgtag tgcgttattt
      841 gtggaagaaa ggcgcagaat atacccaacg cgaatccacg acacgaacca ccttcagctt
      901 caaccttggt gaacagcatc ccgtttatga agtgctggac gataaactgc cggcggtgag
      961 aaagccctac gcatattacg tccgcgtgcc agatgtgccc gcctttttga accaggtgaa
     1021 accagcgctg gagaaacggt taagtaactc tgttgcagta gggtatagcg gagaactacg
     1081 ggttgggttt tataaagatg gtttccgtat ggtctttgaa aacggaaaca tcactttaat
     1141 cgaagcgcta aaacacacta cagatagtga cgtagacatt gccttcccag aatattcttt
     1201 cttgcacgtt ttatttggtc accgtagcct agatgaattg cgtcatgcgt ttgtggattg
     1261 ttatgtacaa aaccccacag cacgtgtatt agtcaacgcg ctttttccga agcgtgattc
     1321 gttcgtgcag gcagtgtcgt aatatgttcc gcatagaagc ctccaaagtg tattatttca
     1381 tcgagttcac atcttcggca ttcttttcga tgatgttcgt ggtgatttct ttatatgaag
     1441 ccaccgtggc tgggttgacc cccatgcagc tcgtacttgt tggaacgact ttggaagttt
     1501 ctgcttttct ctttgaaatt ccaactggtg tggtggcaga tgtcttctca cgcaggctgt
     1561 ccatcatcat tgggtacatt atgatgggag taggcttttt agtcgaaggt ctattcccat
     1621 cttttcttcc gattttgctt gcacaagtga tttggggcgt gggctatacc ttcaccagcg
     1681 gcgccacaca agcttggatt accgacgaag tgggggagga gaaagccaat ccgcttttta
     1741 tgcgggcggc acgagtgggc ttggtcgctt cgttgatcgg gatgggcgcc acaatgctta
     1801 tcggtgcgaa caatgcagct atgccaatta tcgttggcgc cattggtgtg attctgattg
     1861 gcattgtttt gatcttcatc atgcctgaaa cgggcttcaa accaactccc atggagaatc
     1921 gcagcacctg gcaacacatg tggcatacct tcaatgaagg tgtgaagtct gtgaaggcgc
     1981 gcccgcgttt gatgaatatt ttaggcgtgg gctttttcta tggcttgtat agtgaaggct
     2041 tcgaccgtct ttgggtgaag cacttgctcg atacctttca aatcccagtc ctgtttggcg
     2101 gtaatcaagt tgctttcttt tcgctgttga gattttcagc aggcttgctg acagtcttcg
     2161 taatgcagct catagaaaaa cgcgtggatg cgagtagtcc gctttcgatt ggaagggcga
     2221 tgatgattgt gacgggcgtc attgccttgg gcattgtcgg atttgccctc tcctcgcttc
     2281 ttcctttgac gttgggcttg tatctcacca tttacatttt gcgtcaggtg aggattcctc
     2341 tgcaaaccgc ttgggttaat cataagcttg acccgcagac tcgtgccacc gtccactcga
     2401 tgacgggaca agtagattcg attggacaaa ttctgggcgg cgggtcggcg tttattgcgc
     2461 gctatctctc ggtagtggca gccattacaa cttcaggcat tctgcttggg ccagcactcg
     2521 gcttggttcg cagagccaac tcacagtcca cacatgaggc ggaggttgat ccagtagggg
     2581 agttgacagg cgattaaaag tcggaatagc gggggaaatt tgataaatcc tacccataca
     2641 agagtgcaaa caggtttaat tgacgcttta tctgtttacc aagtataatt tcgagcgctc
     2701 cctatgaagg gaatttgagg aattatggct ctgcgcggta atttaagaga ttttacgatc
     2761 actcaacttt tgaacctcat caatctcgcc agcaagacgg gaactttggt gattgatggt
     2821 gcgtcggaac aggcacatat ttcgttccga gagggcaagt tagcttatgc ccgcattggc
     2881 aaggaagatg gaagccttgc tttggttctt tataaggcaa ataagatcaa tgccaatcag
     2941 tacaaagcca ttatcgaccg cgccgggcag atgtctgata aagagttggg gttgttgttg
     3001 atcaatgcag ggtacgtttc tcgtgacgat attctgctga atttgcaggc ttatttcaca
     3061 gacatggttc gccgcatgtt tacttgggtg gaaggcgttt tccgtttcga agcggaaatg
     3121 ctgccccctg atgatcgcat caatgtacgg ctcgaccttg agaatattat tatcgaaggt
     3181 tcacgtcaac tgcgcgaatt ggagcagttg caggatgaaa ttccaagcct ggatatggcg
     3241 ctgaagttca ccgaccgccc gttgaccaat gtcaacttga gcgtggatga atggaaggtg
     3301 gtcaagtttg ttgacccgaa gaatacgatg cgccagatcg cgaagacgaa taagttaacg
     3361 gaagttgaag tacgccgtat tgtttacggt ttattgcaag cgggtcttgt ggaattggtg
     3421 cgccctgccg atgcgcccac gccacaggct gcaaaaccgc tcgctccgca agaaatggaa
     3481 gacaaaaaga atttggtcaa caaactgatc ggccgcattc gttcgttata attttttcag
     3541 gccgaaagga taggaattat gcaaacggtc aaaatggtag tcacaggtcc attcagcgct
     3601 ggcaagaccc agtttattca gtctgtcagt gagatcgatg tggttgccac agaacggaag
     3661 atttcctcag atgaggaacg ctcggtgaaa tctgccacaa ctgtggcgat ggatttcggg
     3721 cgcatcacag ttgaccagga tctggtcctg tacctgttcg gcacccccgg tcagaaacgg
     3781 ttcgacttta tgtgggagat cctttctgaa ggtatgttgg gattcatcgt gatggtggat
     3841 agcacacgcc cagagacctt ccgtgaagcg cgttcgattc tcgaaacctt ccgcgcttat
     3901 gctcctactc ccttcgttgt ggctgcaaat aagcaggata aggaagacgc ttgggacttg
     3961 gaagatatgg ggcatgcctt gcgcctcgat aaaaatacga aactgctgcc ctgcgttgcg
     4021 acagaccgca actccgttaa gaccattttg cttgagctgt tgtacagcat tatgagcgaa
     4081 atggaaggaa aatagtgggc gggtgatgtc ttactgtgtc ttcaattacg actgatcagg
     4141 gaatcgttca ttacgaggtt ttcggtcggg ggaaaccggt catccttttg catgggtatc
     4201 agggttcatg ggggttatgg caggaaacca tggcatactt gggcgcgttt tatcgcacct
     4261 atgctcttga tttttggggt tttggcgaat caggtaccaa gcgttccacc tatgcggtaa
     4321 aagactttgt aagtttggtt taccaattta tggagcagtt gggcatcgtc cgtgcgccat
     4381 tggtggggca cagcatgggc gggacggtca gcttgatgac cgcaatgcag cacccagacc
     4441 ggattcaaaa agtagtggtg atcggttcac ccatctcagg gtcttctctg tatttctttc
     4501 cgcgtgtttt tggttacaag acctttggtt ggctgaccta tcataacctt tggatctata
     4561 aaggttttta tcgaatcctt tctcccttct attcaaaaga taaaaactgg gcaaagatga
     4621 tggaccgtga cgtttcacag gtttccttgg aagcattctt ttcaagcatt ggctccctac
     4681 gccgtacgga cttgcgccca agcttgcata ccattcaagc ccc
//
LOCUS       DAOQNJ010000144         4723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11120_length_4723_cov_8.025064, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000144 DAOQNJ010000000
VERSION     DAOQNJ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11120_length_4723_cov_8.025064"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLG52_05590"
     CDS             <1..618
                     /locus_tag="PLG52_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNB40949.1"
                     /translation="AFVSGLTYDYPEVIRPALQIHHTAKGVARAAGVYIMIVEDKVFL
                     FTDATVNIDPTADDLAEIAILAADYAKKIELDPHVAFLSFSNFGSTPHPLSDKVRKAV
                     NLVKSRRPDLRVDGEMQADTAVVPEIAEERYPFSAVKDANVLVFPSLESANIAYKLLA
                     RLGNAKAIGPILLGVGAPVHVLQTGDDVNAIVQIASVAVMDAMGR"
     gene            737..1978
                     /locus_tag="PLG52_05595"
     CDS             737..1978
                     /locus_tag="PLG52_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB40950.1"
                     /translation="MDNLENIARKITSVLFAQQSLASAGFIAAATLNSIVGKELSHNS
                     SWAGVPTAVYLLAGAFSAFMWGYVYDAIGRRYGLVSGLSTGVVGSSLTFYSIAIHSFP
                     LFLGGMVLMGIANSAVQLGRFAAAEVNKPEHRGQAISNVVIGGTVGSIIGPFVAGPAG
                     NLVGGWGIDELAGAYLVAAVLFVIGAIVVFTGLRPDPREIGKQVAEKYPETLIKHKHV
                     RGLLEIFRQPAALVALLSMVLGQMVMVLVMVITSLHMRGHNHNLSDISAVIASHTFGM
                     YAFSIFSGRLADKWGRGTVILIGSATLVIACIAATISPDVLPLGVALFLLGLGWNFCF
                     VGGSTLLADQLSPAERARTQGFNDLLVGLASALGSLESGFIFASLGYNMMAYVSATIA
                     LIPLLVVVVWMMRTPTKLKPA"
     gene            1978..>2297
                     /locus_tag="PLG52_05600"
     CDS             1978..>2297
                     /locus_tag="PLG52_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40951.1"
                     /translation="MKNILGVRFKLFLLFFAVGTGFTFAIGWLTQFHVTLSDYWNVLY
                     YGRHMTLSQPESLYNGFYPFGYAFLIGQMPLTYVLPLSYLLNALLSGLLVASVSTLVA
                     YTDS"
     assembly_gap    2298..2397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2398..3686
                     /locus_tag="PLG52_05605"
     CDS             <2398..3686
                     /locus_tag="PLG52_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40952.1"
                     /translation="SGLLVASVSTLVAYTDSIPATVIAFYSSIAAPFVFQNANTLGPD
                     IGAAAFIAFAVFLLCHTWLSREDKPLSDTQLILAGVSLGLSFLWRTHAAVAIIAILTG
                     YILIAGIRPFRPLILLAASLAGVAGIQIIVNLVSGHGPLETAQAFNLYKYFYGVNWTN
                     PVGPEVVKDFSLIRTVTNDPTAAFNLYKPFFLYFISHTWAALLAFILLPKGRFSRFSL
                     FSFVFIVLYAIPISLSDSPRAPAVLMSVYLPSLAVLLAVLIEAIKKYFPSIRLGEWIG
                     IGVFIAVAFPTFYSWAVYDVGIAQAAIAERKVLSVIEQTLRTKGLTSSDEIFSDRYDF
                     YTPNTMPYHARGIEKWSVDWFLGMSETYPLLPNDTWESFATACKEEGIRFLVLGPNSH
                     LRGDFFGQIYTDDVNLDALGLQFIGQRGNKRLYEFK"
     gene            3821..>4723
                     /locus_tag="PLG52_05610"
     CDS             3821..>4723
                     /locus_tag="PLG52_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40953.1"
                     /translation="MAKQIADASFALPSQSPYYGPGGIPFAYPPLGLYVLAICIKLTG
                     KYYIFLRFFPPLYSLFAVGLTFLLAQKFFKQPFLSMMVAILTATSVDLYVAHTWSSGV
                     VRALAFIFTILVLYFYSPNPDERTRKDIFLAGLFFGLAGLSHLVYALFCFFWIVSTSI
                     SFRDWKKTITDPLLAGLTALVTISVWVIPVAVRYGWTVFFGAFNSHGGSDLIGEASNF
                     ASLIHLFLINLEPILSNPLLAIWVIVGGVYLLLHKQYHFVAFFLLIVLTFPENARFVY
                     WLGCFFVAYGLWFFANQVYDWGLKK"
BASE COUNT          976 a         1086 c         1050 g         1511 t
ORIGIN      
        1 gccttcgtct ctggcttgac ctatgattat ccagaggtga ttcgcccagc attgcaaatt
       61 caccatacag caaaaggtgt ggcacgtgcc gcaggtgtgt acatcatgat cgtggaagat
      121 aaagtattcc tcttcaccga tgccaccgtg aacattgacc cgaccgcaga cgatttggct
      181 gaaatcgcca ttcttgccgc agattacgcc aagaagatcg agcttgaccc gcatgtggca
      241 tttctttcat tctcaaactt tggttcaacg cctcatccgc tttcagataa ggttcgcaag
      301 gcggtcaacc tcgtcaagtc tcgccgacct gacttgcgcg tggacggtga aatgcaagcc
      361 gatacggctg tggtgcctga gatcgctgaa gaacgctatc cgtttagtgc ggtgaaagat
      421 gccaatgtat tggttttccc atctcttgaa tcagcaaaca tcgcatacaa gttgctggct
      481 cgtctgggca atgccaaggc aatcggaccc atcctcttgg gcgtcggcgc tccagtccat
      541 gtcctccaga ccggtgacga tgtcaacgca attgtgcaga ttgcttccgt agctgtgatg
      601 gatgcgatgg gaagatagtt ctatagttgg ctagtcaaat catgtttaag accccagtaa
      661 tctttactgg ggtcttttct tctctccgct tcaaaagtgt ataatccaaa ccacctcacc
      721 caaaggaaat gatttcgtgg ataatctcga aaatatcgcc cgtaagatca catccgtatt
      781 atttgctcaa caatcccttg cttctgcggg gtttatcgca gcggcaacct taaactccat
      841 tgttggcaaa gaactaagtc ataactccag ttgggcaggt gtgcccacag ctgtttattt
      901 attggctgga gcgttttctg cttttatgtg gggatatgtt tacgatgcca ttggcagacg
      961 atatggactc gtcagcgggc taagcacagg tgttgtcgga tcaagcctga ctttttactc
     1021 gatcgccatc cattcttttc ccttgttcct aggcggaatg gttctcatgg gcattgcaaa
     1081 ctcagcagtg caacttggtc gctttgctgc cgcagaagtt aacaaacctg agcatcgtgg
     1141 gcaagccatt tcaaatgtcg ttatcggcgg gacggttggt tccatcatcg gtcccttcgt
     1201 ggcgggacct gccggaaacc tcgtcggcgg ttggggcatc gacgaattgg ctggcgcata
     1261 tcttgtcgca gcggttttgt tcgtgatcgg agcgatcgtt gtcttcaccg gtttgcgccc
     1321 agacccgcgc gagatcggca aacaggtggc agaaaaatat ccagaaacgc tgatcaagca
     1381 taaacacgta cgaggcttgc ttgaaatttt ccgtcagcct gcggcattgg tcgccttgct
     1441 aagtatggtg ctgggtcaaa tggtgatggt gttggtgatg gtcatcacat ccctgcacat
     1501 gcgcggacat aaccataacc tctccgatat ttctgccgtg attgcctctc acacctttgg
     1561 tatgtatgca ttttcaattt tttccgggcg ccttgccgat aaatgggggc gtggcacggt
     1621 gattttgatt ggttcggcaa cactggtcat tgcctgtatt gcggctacga tctcaccaga
     1681 tgttcttccg cttggcgtgg cattgttctt gcttggtttg ggttggaact tctgctttgt
     1741 cggtggttct accttgctcg ccgaccaact ctctcccgcc gaacgcgccc gcacccaagg
     1801 cttcaatgac cttctcgttg gtctcgcatc tgctcttgga agtttggaaa gcggttttat
     1861 ctttgcttca ctcggctata acatgatggc atatgtcagc gccaccatag ccttgattcc
     1921 gcttttggtg gtcgttgttt ggatgatgag aacccccaca aaactcaaac ctgcttgatg
     1981 aaaaatattc tcggtgtacg ctttaaatta tttttgttat tctttgctgt tggcacaggg
     2041 ttcacctttg cgattggctg gctaactcaa ttccacgtaa ctcttagcga ttactggaac
     2101 gttctgtatt acgggcgtca catgactctc tcgcagccag agtcactgta caacggtttc
     2161 tatccctttg gatacgcctt cctcattggg cagatgccgt tgacctatgt cctgccgctt
     2221 tcttatttat taaacgcgct tctttctgga cttttagttg cctctgtttc gacacttgtt
     2281 gcctacaccg attccatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt
     2401 ctggactttt agttgcctct gtttcgacac ttgttgccta caccgattcc attcctgcca
     2461 cggttatcgc gttttactct tctattgctg ctccatttgt cttccaaaat gccaacaccc
     2521 tcggacccga tattggagcc gcggcattta tagcatttgc tgtctttttg ctgtgtcata
     2581 catggttaag tagagaagat aaaccactat cagatacaca gcttattctg gctggtgtct
     2641 ctctgggtct ctccttcctc tggcggacgc atgctgccgt tgccatcatt gccattctta
     2701 ccgggtacat tctcatcgct ggaattcgcc catttcgccc attgattctg ctagcagcct
     2761 ctttagcagg tgtagcgggc attcaaatta tcgtcaacct cgtttctggg catggtccgc
     2821 ttgagaccgc ccaagccttt aatctttaca aatacttcta tggcgtaaac tggacgaacc
     2881 cagtaggtcc agaagtggtc aaagattttt cgttaatccg aacagttacg aatgacccta
     2941 cagcagcttt taatttatat aaaccatttt tcttgtattt catttcccat acttgggctg
     3001 ctttgcttgc atttatcctt ttgccgaaag ggcgtttttc ccgcttttct ttattttcct
     3061 tcgtttttat tgttttgtat gcaattccga tctcgcttag cgatagccct cgtgcaccag
     3121 ctgttttgat gagcgtgtat ctcccatcgt tagccgtttt gttagccgtt ttgatcgaag
     3181 ctattaagaa atattttccc tctattcgtt tgggtgaatg gattggaatt ggagtattta
     3241 ttgctgtcgc ctttccaaca ttttatagct gggctgtata tgatgtcggt atagcacagg
     3301 ctgccattgc cgaacggaaa gttctttctg ttattgaaca gactcttcgc acaaaaggtc
     3361 tgacttcttc tgatgaaatt ttctctgatc ggtacgattt ttataccccg aataccatgc
     3421 cttatcatgc tagagggatc gaaaaatggt ccgtcgattg gttcttggga atgagtgaaa
     3481 catatccatt attgccaaat gacacatggg aatcatttgc cactgcttgt aaagaagagg
     3541 gaattcgttt tctcgttctt ggcccgaata gccatcttcg tggagatttc tttggtcaaa
     3601 tctataccga tgatgttaac cttgatgctt taggcttgca atttattggg caaaggggca
     3661 ataaaagatt gtacgagttc aaatgatctg gattacaaaa ttaaaatgga ctgattccaa
     3721 aattgctttt ttgattgcaa tgttgggttc tcttccttta cttttttata acgcattttt
     3781 acatgacttt ccaatggggt atgctgggtt gtttacacaa atggcaaaac aaattgcgga
     3841 cgcgagcttt gctttgccca gtcaatctcc atattatggt ccaggtggca ttccatttgc
     3901 ctatccacca ttgggcttgt atgtacttgc tatatgtatc aagttgacgg gtaagtatta
     3961 catcttcctt cgctttttcc caccacttta tagtctcttc gcggttgggt tgaccttcct
     4021 cctcgctcaa aaatttttca aacagccttt cctttcgatg atggtcgcga tcttgactgc
     4081 aacctcagtg gacttatatg tagcacatac ttggtcttcg ggtgttgtac gtgcccttgc
     4141 ttttattttt acgatcttgg ttttgtattt ctactcccct aatcctgatg aacggacgcg
     4201 gaaagatatc tttttagcgg ggctgttttt tgggctagct gggctgagcc atctggtata
     4261 tgcattgttt tgcttctttt ggatcgtgag cacctcaatt tcatttcgtg attggaagaa
     4321 gacaatcaca gatccattac tggctggttt gacggcgctg gttactatca gtgtttgggt
     4381 catccctgtt gcagttcgtt atgggtggac ggttttcttc ggagccttca attctcatgg
     4441 ggggagtgat ttgataggag aggcatcaaa cttcgccagc cttattcatc tgttcctgat
     4501 taatctcgag ccaattctgt caaatccact tttggcgatc tgggtgattg ttggcggggt
     4561 ttatttgctt cttcacaaac aatatcattt tgttgccttc tttttgctta ttgttttaac
     4621 tttccccgaa aatgcccgtt ttgtatattg gcttgggtgt ttttttgtcg cctatggatt
     4681 gtggttcttt gctaatcagg tatacgattg gggattgaaa aaa
//
LOCUS       DAOQNJ010000145         4710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11162_length_4710_cov_8.004941, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000145 DAOQNJ010000000
VERSION     DAOQNJ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11162_length_4710_cov_8.004941"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="PLG52_05615"
     CDS             complement(<1..1912)
                     /locus_tag="PLG52_05615"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNB40954.1"
                     /translation="MSDSAHQEKHFESYIVSKLVEQGWKVGDTKGYDTERALYPEDLI
                     AWLEGSGQEAKWEKLKKDNADRATDVLMDRLAKALETHGTIHVLRQGFAIAGCGQIDV
                     SEAAPEDKRNQTVVARYAANILRVVPQLKYHPARELAIDLVFFINGIPVATVELKTDF
                     TQSAEAAMDQYRNDRIPFDPKTKRKEPLLTFKRGAIVHFAMSDSVIHMTTKLDGENTF
                     FLPFNKGNDGRAGNPEGEIRPDGVRDYPVAYFWESVCQRDAWMRIFHSFIYVEKKSVA
                     DLKGNWKKKETLIFPRFHQWAAVNKMIEDARLNGPGFSYLAEHSAGSGKTSTISWTAH
                     DLVKLRRDNGEAIFDSVIIVTDRTVLDDQLQEAVKQIDHQFGVIAAIDKKKSSKSKSK
                     QLAEALLAKTPIIIVTIQTFPFAMEAIVTEKSLKGKSFAVIIDEAHNSQTGSTAAKLQ
                     TALTMSGEGKMASLTVEEILELLQKSRARPKNISYFAFTGTPKHSTLMLFGRTPDPSR
                     PPSDDNLPEAFHKYRMRQAIEEGFILDVLQGYVPYRTAFNLAKQVEDTKRVNDRAAKR
                     ALAQWMSLHPTNVTQKVQFIIEHFSKNVMHRLDGKAKAMVVTSSRAAAVRYKKSFDKY
                     IEKHPEHSGIRSL"
     gene            complement(2269..3821)
                     /locus_tag="PLG52_05620"
     CDS             complement(join(2269..3353,3353..3821))
                     /locus_tag="PLG52_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611769.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNB40955.1"
                     /translation="MPRYSEARKDAVLRRMMPPENLPLSQVAKETGISEPTLYNWRKQ
                     AKARGLAVPGNGQTPENWSSADKFAVVLETSRLNETELSEYCRKKGLMPEQVTRWSKA
                     CEQANASAASQEKAAAAHRREDKTRVKHLERELARKEKALAEAAALLVLSKKYQGVLE
                     QGRRGRLIPLQERERYVEWIGLAQQAGARQKEACAVLGISERTLERWLNEGVIKADGR
                     PGAIRPAPTNKLSEEEKQEILAVVNEPRFASLPPSQIVPCLADEGRYLASEASFYRTM
                     KEAGQQHPRGHAKPPVRRAPASHCATGPNQLWCWDITYCASRVRGLYFYLYVVMDVYS
                     RKIVGWDVHETESGEHASVLIRRCVMSEGIAGHVPLILHADNGSPMKSGTLLATLQWL
                     GVTPSYSRARVSNDNAYVESLFRTCKYRPEYPRDGFENLDSVRQWMLTFVAWYNKQHR
                     HSGLKFITPEERHSGRSEKVMAQRKKLYMQAQAKHPERWSGTTRDWDLPEKVWLNELK
                     NQEQKKEAA"
     gene            complement(3880..>4710)
                     /locus_tag="PLG52_05625"
     CDS             complement(3880..>4710)
                     /locus_tag="PLG52_05625"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNB40956.1"
                     /translation="DKAWGRISRADHERYSKKYRPMLNDIYMVKSGATTGVTAIVEDS
                     REFNIWSPLAAIRSNEKVLPRFLLNALRSASFKDGVALNWSYGTQQNIGMKVLENLPV
                     QLPPLSEQVDIIETIDRETARIDALILKKSRFVELLEEKRLTVITTAVTKGLNSGVKM
                     KASGVEWIGAVPKHWAVCKLSYRYSVELGKMLDEKKMTGKNPVPYLRNKDVQWQQINV
                     NDLPIIDITPQEIDRYTVKSGDLLVCEGGDVGRAAIWRGDDNVIGYQKALHRLPPVSG
                     "
BASE COUNT         1027 a         1263 c         1094 g         1326 t
ORIGIN      
        1 ccagagagcg aatccctgaa tgttccgggt gcttttcgat atacttgtca aagctctttt
       61 tgtaccgaac tgccgctgct ctggagctgg ttacgaccat cgccttggct ttgccgtcca
      121 gacggtgcat cacgttctta ctgaagtgtt caataatgaa ttgcaccttt tgcgtgacgt
      181 tggtcggatg caaggacatc cactgggcca aagccctctt tgctgcccgg tcattgaccc
      241 gcttcgtatc ttcgacttgc tttgcaaggt tgaacgctgt cctgtagggc acatagcctt
      301 ggagcacatc cagaatgaat ccctcttcaa tcgcctgcct catgcgatac ttatgaaagg
      361 cttctggcag gttatcgtca gatggcggtc tcgaaggatc tggggtacgc ccaaagagca
      421 tgagcgtgga atgctttggt gtgccggtaa aggcgaagta gctgatgttc ttcggacggg
      481 ctctggactt ctgtagaagc tcaagaatct cttccacagt caatgaagcc atcttccctt
      541 cgccactcat tgtcagtgcc gtctggagct ttgcagcggt tgagcctgtc tgtgagttgt
      601 gagcttcgtc gatgatgacg gcaaaactct taccctttag cgacttttcc gtaacaatag
      661 cttccatggc gaaggggaat gtctgaattg tcacgatgat gattggtgtt ttggcaagca
      721 aagcctctgc tagttgcttg ctcttggact tcgaagattt cttcttgtcg atggccgcga
      781 tcacgccaaa ttggtgatca atttgcttta ccgcttcttg gagttgatca tccaaaacgg
      841 ttctatcggt aacaataatt acgctatcga aaattgcctc accattatca cggcgaagtt
      901 ttacgagatc gtgagccgtc caggaaatcg tacttgtctt acctgatcca gcgctatgtt
      961 ctgccaagta agagaatcca ggaccgttta acctggcgtc ttcgatcatt ttgttgacag
     1021 ccgcccattg atggaatcga gggaaaatca gggtttcctt tttcttccaa ttacccttta
     1081 ggtcagcaac acttttcttc tcaacataga taaagctgtg gaaaatacgc atccacgcat
     1141 cacgctggca gacggattcc caaaaatacg ctacgggata atctcgcact ccatccgggc
     1201 ggatttctcc ctcaggattg cccgctctgc catcattacc cttgttgaag ggtaggaaaa
     1261 atgtgttttc accgtcgagc ttcgttgtca tgtggatcac ggagtccgac atggcaaaat
     1321 ggacgattgc gccacgcttg aacgttaaaa gtggctcttt tctcttggtc ttcgggtcaa
     1381 acgggatgcg atcattgcga tactgatcca tcgcggcttc agcggattga gtgaaatctg
     1441 ttttcagctc gaccgtagcg acgggtatgc cgttaatgaa aaacaccagg tcaatcgcaa
     1501 gctctcttgc tgggtgatat ttcagttgag gtacaaccct tagaatgttg gctgcatacc
     1561 ttgcaaccac cgtctgattg cgtttgtctt ccggcgcagc ttcggacaca tcaatctgcc
     1621 cgcagcctgc aatagcaaac ccttgcctga ggacgtgaat cgttccgtgg gtttcaagcg
     1681 cctttgccaa acgatccatc aaaacgtcgg ttgcacgatc agcattatct ttcttgagct
     1741 tttcccattt tgcctcttgc ccagaaccct ccaaccaagc gatcagatcc tcaggatata
     1801 gcgctctttc cgtgtcgtaa cctttagtgt caccaacctt ccagccctgc tcgaccaact
     1861 tggagacgat gtagctctca aaatgctttt cttggtgggc actatcgctc atacagattt
     1921 cctaaggtca atttggccgg tgactgcggc ggtaatgagg gcggcgcgcc gttcttttag
     1981 aagatcgatg ctattttggg ttttctttat gagagtgtcc aagcgcttca attctgagga
     2041 gaggtactga acaatttcct gttgctcgct tagtggcgga aacgcaaatc tatattcgcg
     2101 aaacttttcg gcaggcaaat gagaaattgt cgacttagta tcgctctcat cgaaaacccc
     2161 attatttttc gccgcaaaaa gcgcataaaa atagaactcg accgtatccg tctttttgga
     2221 tctagctcgt agacggtgat tttcaagata gttgtcgggt aaaaatgttc aagcggcttc
     2281 tttcttttgc tcctggtttt tgagttcgtt taaccagacc ttttcgggca aatcccaatc
     2341 tctggtcgta ccactccaac gctctggatg ttttgcctgg gcttgcatat aaagcttttt
     2401 tcgttgcgcc atgacctttt ctgaacgacc cgagtgccgc tcttcgggtg ttatgaattt
     2461 caaaccgcta tggcgatgct gtttgttgta ccatgccacg aatgtcagca tccattgacg
     2521 aacgctatca aggttttcaa aaccatcccg aggatattcc ggtcgatatt tgcaggtgcg
     2581 aaacagcgac tcaacataag cgttatcatt gcttacccgt gcacgactgt aggaaggtgt
     2641 cacacccagc cattgcaagg tggccaatag cgtacctgat ttcatcggtg atccgttatc
     2701 tgcatgcaat atcaatggca cgtggccggc aataccttcc gacatcacgc atcgtctgat
     2761 cagcacgctg gcatgttctc ccgattccgt ttcgtgtacg tcccagccca caattttgcg
     2821 gctgtagaca tccatcacca cgtataggta aaaatacaaa ccacgcacgc ggctggcgca
     2881 gtacgtgatg tcccaacacc acaattgatt gggcccggtc gcgcaatggg atgccggtgc
     2941 ccgccgcact ggcggcttcg catggcctcg cggatgttgc tgaccggctt ccttcatcgt
     3001 cctgtagaaa ctggcttccg aggccaggta gcgtccctcg tcggccaaac aaggcacaat
     3061 ttgagacggt ggcaggctcg caaaacgtgg ctcattgacc acggccagaa tctcttgttt
     3121 ctcttcttca ctcagcttgt tcgtgggggc aggacgtatc gccccaggcc ggccgtcggc
     3181 tttgattacg ccctcgttca gccagcgctc caatgtgcgc tcgctgatgc ccagcaccgc
     3241 acaggcttct ttttggcgtg caccggcctg ctgcgccaaa cctatccact ccacgtaacg
     3301 ttcgcgctcc tgcaagggaa ttaagcgtcc tcgccttcct tgttcaagaa cgcctgatat
     3361 ttttttgaga gcaccagcaa tgctgcggct tctgccagcg ctttctcttt gcgcgcaagc
     3421 tcccgctcca gatgttttac tcgggtttta tcttcacggc gatgagccgc cgccgctttc
     3481 tcctgactgg ccgcgctcgc attggcttgt tcacacgcct tgctccagcg agtcacttgt
     3541 tccggcatca gtcctttctt tcgacaatac tcggatagct cggtctcgtt taaccggctg
     3601 gtctccaaca ccactgcaaa cttgtccgcg gagctccaat tctccggtgt ctgtccgttg
     3661 cctggcacag ctaatcctct cgctttggct tgtttccgcc aattatacaa ggtcggctcg
     3721 cttattccgg tttctttcgc tacctgcgat aacggtaaat tctccggcgg catcatccga
     3781 cgtaacacgg cgtcttttct tgcttctgaa tagcggggca tttcttttgc cttccgcccc
     3841 ccgtaaattt agagaagctc tcgatgtgag agccgacaac tatcctgaca cagggggtag
     3901 acgatgcaat gccttttgat agccaataac gttatcgtcc ccacgccata tcgcggctct
     3961 tccgacatca ccgccttcgc atacaagcaa atcgccactc ttaacggtat accgatctat
     4021 ctcctgagga gttatatcga ttattggcag gtcattgacg ttaatttgct gccactggac
     4081 gtccttattt ctgagatatg gaacaggatt cttgcccgtc attttttttt cgtcgagcat
     4141 ctttccgagt tctacggaat atcgataact aagcttgcag actgcccaat gctttggcac
     4201 agcgccgatc cactcaacac cagaagcctt cattttcacc ccggaattta atcctttggt
     4261 gacggcagta gtgatgacag tcaaccgctt ttcctccaaa agctcgacaa atctagactt
     4321 tttaaggatt aatgcgtcga tcctcgcggt ctcacggtcg atagtttcga taatatcaac
     4381 ctgctcgctc aatggaggaa gttgaacggg gaggttttca agcaccttca tccctatgtt
     4441 ttgctgtgta ccataactcc agttcagggc aactccgtct ttaaatgatg cggatcgaag
     4501 agcgttgaga aggaatctcg gcaaaacctt ttcattcgac cgaattgctg ctagtggtga
     4561 ccaaatgtta aattcacgac tatcctcaac aatggctgta actcccgtgg tggcaccaga
     4621 cttaaccatg tatatatcgt taagcattgg tctatatttt ttggaatagc gttcatgatc
     4681 agctcgggat atgcgccccc atgccttatc
//
LOCUS       DAOQNJ010000146         4680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11268_length_4680_cov_7.725189, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000146 DAOQNJ010000000
VERSION     DAOQNJ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11268_length_4680_cov_7.725189"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLG52_05630"
     CDS             complement(<1..559)
                     /locus_tag="PLG52_05630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HNB40957.1"
                     /translation="MTQTLGDYLIHKIPKLPDDLTQQDAQLLLAHVIGRSRTWLLAHL
                     DTPLSATLLDSANQAFTRLEAGEPLPYILGHWEFFGMDFDITPDVLIPRPETETMVEK
                     AIKWLSASGERRTVADIGTGSGIIATSIAMHVPNANILATDISRAALKVAKHNAEKFN
                     VQHRIDFLECDLLPEHMDPLPTDRHF"
     gene            complement(556..1407)
                     /locus_tag="PLG52_05635"
     CDS             complement(556..1407)
                     /locus_tag="PLG52_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNB40958.1"
                     /translation="MRKISLPRQIATQLIFLLVGLFILIPVWSTFRLAFDGSLRGRPT
                     EFTWLPKEFSAEAFTKVLDRPYQSVEFNILLRNSMIVSIGAAVLAVILGITLAYGFAR
                     FRFPAKQTGLFVLLLAALLPPIAFATPLYIILSLLKIRTTLLGLVIVYAAFAMPFAIW
                     NMRATFAAIPKELEEAAYLDGANHYQTFLNISLPISLPSISVTAMIAFLRGYTEFAIG
                     WLFVDKADNVTLSMAVYALVNGQFSGAQPWSYLGSLAIIMSIPVMVIFIFFQETLMER
                     LLFQETS"
     gene            complement(1400..3511)
                     /locus_tag="PLG52_05640"
     CDS             complement(1400..3511)
                     /locus_tag="PLG52_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB40959.1"
                     /translation="MKNYQLPLRILSIVVVLIIAGMTRARAVQHLNIDFDEDDYTRAA
                     QEFAHLIRTSNWSGFLETNYRPEHPPLAKIVMGISLLTTPEKDLIADRPTSAEPNKYL
                     PRVILKPERTVNAVFGWLTVALLTIVNPLAGLSLATHSITVKYNSQAMLEALPALTSF
                     CMVLAYLQFKKNHNTRWLITSAVFLGLTAASKYLYCVIALAILADWFLTSKEQEELKS
                     FFKQALIWGTLGILIFFVTNPFLWANPIQRLRESVLYHAGYSSSSEEVKDAGFPVWQP
                     VQWLVTSAADWEEDAFFTGPDLLIAILAAFGFARLWKRERLYVLWFGFALLFLLLWST
                     KWPQYILILTVPLSLAAAEGLTQIWQNFLEWLKAPKTVYNKKESRQAIPWLVPGLIVF
                     TVLTLLPFFFQLGVSVTDFSSQSIRDGFQGGIWREVSGGLTGQITPTSPEAAYNSNKV
                     QYIGFQAYQPIFEYIVSVNKIFVFNILWMVVSTSIQVLLGLGLAILLNQRGVKLGKFW
                     QTLFILPWAIPEFIGALMWLNIFVPEIGWLSLAAKKYGDAFPLQSLIGWQSTPSLWFL
                     VLLIPAIWYGTPLIMLASNASLKTIPKEIFEAASLDGANSWQILQHMVLPLIRPVLLP
                     AIIIRGIYAFNQFYLFQAMPFSEGTLANFSYNVFNPSSRFGGGQFAISAVINIITVLI
                     LIVLVSAFNRRMQTREEFINA"
     gene            complement(3608..>4680)
                     /gene="prfA"
                     /locus_tag="PLG52_05645"
     CDS             complement(3608..>4680)
                     /gene="prfA"
                     /locus_tag="PLG52_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNB40960.1"
                     /translation="DKLQAIEEKFEQLGQELLVVGTDYKRAAEISKERTSLEPLIEKA
                     KEYRQAVKSLEEAKEMLISESDAELLALAKADIADLEPKIEELEKVIKGLLVPKDPRD
                     DKNVIVEIRAGAGGDEAAIFAADLFRMYTRYTDEKKWKTEIMSENAIGVGGYKEVIFE
                     VKGKGAYSKLKYESGVHRVQRVPATESAGRIHTSTATVTVMAEVEEVDVVIPETDIEI
                     EVYRSSGAGGQNVQKNSTAVRLYHKPTGMIVTCQDERSQLQNKLRALSILRARLFEIE
                     EAKRQAELEADRRSQVGSGDRSEKIRTYNYPQSRVTDHRIGLSNYNLAGVMDGDIEGF
                     IDELTTRDEAGKLASTGVEDDE"
BASE COUNT         1220 a         1027 c         1260 g         1173 t
ORIGIN      
        1 caaaatggcg gtcggtgggt agtgggtcca tgtgttcggg cagcaagtca cattctaaaa
       61 aatcaatgcg atgctggacg ttaaactttt cggcgttatg ttttgcgacc ttgagcgccg
      121 cacgagaaat atcggtggct agaatgtttg cattcgggac atgcatggca atggatgttg
      181 cgataatgcc agaacctgtt ccaatatcgg cgacggttct tctctcgccc gaagcggata
      241 gccacttaat ggctttttct accatcgttt ctgtttctgg tctcgggatg agtacatcgg
      301 gtgtgatgtc gaagtccatg ccgaagaatt cccagtgacc gaggatgtac ggcaacggtt
      361 cacccgcttc gagccgggtg aaggcttggt tcgctgaatc aagtagcgtc gcagaaagag
      421 gcgtgtcgag atgggcgagc agccatgtgc gtgaacgtcc gatcacatgc gcaagcagga
      481 gttgcgcatc ttgttgggtc aggtcatctg gcagttttgg aattttgtga atgagataat
      541 cgcccagagt ttgggtcatg aggtttcctg aaagagtagg cgttccatga gggtttcttg
      601 gaaaaagatg aaaatgacca tcactggaat ggacatgatg atggcgagtg agccaagata
      661 gctccatggt tgtgcgccag agaattgtcc gttgacgagg gcgtagactg ccatggaaag
      721 tgtgacgtta tcggctttat ctacaaagag ccagccaatg gcgaattcgg tgtatcccct
      781 gagaaaggcg atcatggcgg taacagaaat agacggcaat gagatgggca ggctgatgtt
      841 gaggaaggtt tggtagtgat tcgctccgtc gaggtaggcg gcttcttcta attcttttgg
      901 aatggcggcg aaagtggcac gcatgttcca aatggcaaag ggcatggcga acgcagcata
      961 gacgatgacc aagccgagca aagttgtgcg aatcttcagc agacttaaga tgatgtacaa
     1021 cggagtggca aatgcaatgg ggggtaacaa tgccgctagc aatagaacga acaagccggt
     1081 ctgcttggca ggaaagcgaa aacgcgcaaa cccataggcg agtgtgatgc ccaatatcac
     1141 tgcaaggact gccgcaccaa tcgaaacgat catactgttg cgaagtagga tgttgaactc
     1201 tacggattga taggggcgat ctaatacttt cgtgaaggct tcagcggaga attctttggg
     1261 cagccatgta aactcggtag gacgtccgcg cagggagcca tcgaaggcga gacggaaggt
     1321 gctccagacc gggatgagga tgaagagacc gaccaacaag aagatgagtt gggttgcgat
     1381 ctgacgaggc agggatattt tacgcattga taaactcctc gcgagtttgc atgcggcgat
     1441 tgaaggcgct gaccaagacg atcaggatta ggaccgtgat gatgttgata acagcagaga
     1501 ttgcaaattg acctccgcca aatctgctgg aaggattgaa tacattgtaa gaaaagtttg
     1561 ccagagtacc ttcgctgaat ggcatagctt gaaatagata gaactgattg aaggcataaa
     1621 taccgcgtat gataatggca ggtaacaaaa caggtcttat caacggcaag accatatgct
     1681 gtaagatttg ccaactattg gctccatcga gagaggcggc ttcaaagatt tctttcggta
     1741 tggttttgag gctcgcattt gaagcgagca taatcaaagg tgttccgtac catatggcag
     1801 gaataagcaa aaccaagaac caaagactgg gtgtgctttg ccaaccaatt agagactgca
     1861 gtggaaaggc atcgccatac tttttggcgg caaggcttaa ccacccaatt tctggcacaa
     1921 agatgttcag ccacatcaaa gcgccaataa attcaggaat tgcccaaggc agaatgaaaa
     1981 gagtctgcca gaacttgcca agttttaccc cgcgttggtt aagcaaaata gcaagcccta
     2041 accctaaaag gacttggata ctcgtagaaa ccaccatcca aagaatgttg aaaacaaaaa
     2101 ttttatttac tgaaactata tactcgaata taggttgata agcttggaaa ccaatatatt
     2161 gaactttatt tgaattgtaa gcagcttcag gagatgttgg tgtaatttgt cctgtaagcc
     2221 caccacttac ttcgcgccag atgccgcctt ggaatccatc acggatggac tggctgctaa
     2281 agtctgtaac tgaaacgcca agttgaaaga aaaacggaag cagggttaaa acagtaaaca
     2341 caatcaagcc cggcacaagc cagggaatag cttggcgtga ttcttttttg ttgtacacag
     2401 tctttggggc tttgagccat tcaagaaagt tttgccatat ctgtgtgagt ccttccgcgg
     2461 cggcgagcga aagcgggacg gttagtatca gaatgtattg cggccacttg gtagaccata
     2521 aaagcaaaaa caacaaggca aagccgaacc acaatacata caaacgttct ctcttccaca
     2581 atcgtgcaaa cccaaatgcg gcaaggattg caatcagaag atctggtcct gtaaagaaag
     2641 catcttcttc ccagtctgcg gcgctggtta ccaaccattg gacaggctgc caaacgggaa
     2701 acccagcatc tttgacttct tctgaacttg aagaatagcc agcatgataa agaaccgatt
     2761 cacgcagtct ttgaattgga tttgcccaga ggaatggatt cgtcacgaag aagatgagaa
     2821 tccctaacgt gccccagatc aaggcttgct tgaagaatga tttgagctct tcttgttctt
     2881 tcgatgtcag aaaccaatcc gcgagaatag cgagagcgat gacgcaataa agatactttg
     2941 aggcggcagt aagaccaaga aagacagccg atgtaattaa ccagcgggta ttgtgattct
     3001 ttttgaattg taagtacgcc aataccatgc agaaactggt caatgctggc aatgcctcta
     3061 gcatggcttg cgaattgtat ttaacagtga tgctatgcgt agcgagactt aacccagcca
     3121 atgggttgac gattgtcagc aatgccacgg ttagccagcc gaagacggcg ttgaccgtcc
     3181 gctcgggctt gaggatgaca cgcggcaaat atttattggg ttcagctgaa gtggggcgat
     3241 ctgcgatcag gtctttttct ggggtagtga gcaggctaat gcccatgacg atctttgcca
     3301 gcggggggtg ctcagggcga tagttcgttt ccagaaagcc agaccagttt gaggtccgga
     3361 tgagatgcgc gaattcttgg gcggcgcggg tgtaatcgtc ttcgtcaaag tctatgttga
     3421 ggtgttgaac tgcgcgggcg cgagtcatcc cagcaatgat aaggactaca acaatgctca
     3481 agatgcgaag ggggagttgg tagttcttca tggcgatgga ttgtatcctc ttcttccgcc
     3541 tgaattgtaa taccgtagag tggattattg aaaacaggct ggagttctcc agcctgttgt
     3601 gagaatttta ttcgtcgtct tcgacgccag tggatgcgag cttcccagct tcgtcgcgtg
     3661 tggtgagttc gtcgatgaag ccttcgatgt cgccgtccat cacaccggcg aggttgtaat
     3721 tcgataaccc gatgcggtga tctgtaacgc ggctttgtgg atagttgtag gtacggattt
     3781 tttcagagcg atcgcccgag ccgacttgcg agcgacggtc tgcttcgagt tcggcttggc
     3841 gtttggcttc ttcaatttca aacaaacgag cgcgcaagat cgaaagcgca cgcagtttgt
     3901 tttgcaactg tgagcgttca tcctgacagg tgacgatcat gccagtgggt ttgtgatata
     3961 agcgcacggc agtggagttc ttttgcacgt tctgaccacc cgcaccagac gaacgataga
     4021 cttcgatctc gatatcggtt tcagggatga cgacatccac ttcttcgact tccgccataa
     4081 cggtgacggt ggcggtagag gtatgaatcc gcccagcgga ttcagttgcg gggacgcgct
     4141 gtacgcgatg cacacccgat tcatatttca gcttggagta agcgcctttg ccctttactt
     4201 cgaagatgac ttctttatat ccacctacgc caatggcgtt ttcagacatg atctcagtct
     4261 tccatttttt ctcatctgta tagcgggtgt acatgcggaa gagatctgcg gcgaagatgg
     4321 cggcttcgtc accgcctgcg cctgcgcgaa tttccacgat gacgttctta tcatcacgtg
     4381 ggtctttcgg cacaagcaag cccttgataa ctttttcaag ttcttcgatc ttgggctcga
     4441 ggtctgctat atctgctttg gcgagagcga gcaattcggc gtcgctttca gaaatcagca
     4501 tttccttggc ttcttccaag ctcttcactg cctggcgata ttcctttgct ttttcgatca
     4561 agggttccaa gctggtgcgt tccttgctaa tctcggcggc acgtttgtag tctgtgccga
     4621 cgacaagcag ttcctgccca agctgttcaa atttttcttc aatggcttgt aatttatcta
//
LOCUS       DAOQNJ010000147         4668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11309_length_4668_cov_9.095166, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000147 DAOQNJ010000000
VERSION     DAOQNJ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11309_length_4668_cov_9.095166"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PLG52_05650"
     CDS             complement(<1..997)
                     /locus_tag="PLG52_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40961.1"
                     /translation="MKKQLFKFTAMFMIAFLLLPLRTAKAHPADVYAHTITVNLTQTQ
                     MTIKWQIKPGPLLVNFIWNEMDSNQDGTVSEDEAQTWSASRLDSFLVSLDGKTLPLTV
                     DSINIPSSMQAFQAGEEFIIYNLSADLSQSTNNTQRIVIENGMEAAKSINWFYLTAEE
                     GTAFLFPAQRSHILTIDYIHNPASIDDQSRLLTTWDSGAPALPFGQKKDVVTETAEQV
                     VPELSQRSPQEILLDLVRSKELSLTFYLFALLIALALGALHALTPGHGKTVVAAYLVG
                     SRGTSWHAVVLGTVVTLTHTGSVFLLGILTLAASKYFLPTAVIPFLEILSGALILG"
     gene            complement(1071..2432)
                     /locus_tag="PLG52_05655"
     CDS             complement(1071..2432)
                     /locus_tag="PLG52_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40962.1"
                     /translation="MKTHLSIDTLAVSVMIALTIIIGIVILIGEGAGIQVRVDLPEDG
                     VVGPFEAVTFTFPEKISAEIASDLISLEPIHEGYLSAVDDYSVRFIPLKPYEQGVTYT
                     FKVSPGEINATTREVKKTQSWQFHVREPRVAYVVSEAGQSSIWSMTKLGSNVKRLTDE
                     TIKVISFDVAQSGDFIVFSSANQNGGIDLWRVSREGGDASMLLDCGLDRCTTPVIAPG
                     DKRIAYSREAAGPTPDLPFGSPRIWVVDLESGADQAIYEDQSILGYNPSWSPDANRLA
                     SFDGLADFIHMIDFKTGDQFLFPSTTGGPVTWSPDSNQFLYTTFTQTEEGGRTQIKLA
                     DLTTNETITLIGEKDTYDYAYYSVAWSPLAERAVLSLRADEEKPTQVLWVFDPNLLDG
                     IIVANDPEYTYNSPQWDPWGNALLFQQFKLKGQYRPEIGLWQEGTIQSEVIAEGILPQ
                     WLP"
     gene            complement(2429..3178)
                     /locus_tag="PLG52_05660"
     CDS             complement(2429..3178)
                     /locus_tag="PLG52_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HNB40963.1"
                     /translation="MPQRMFRSYQGLLLFGLFIFFVFKAVNGQLTWYINSRFVPLTIA
                     GMIFLGILAQTVFSENRRSRKHEEEHPEHHHEHDHAPSSVTLWVMLVPLLIGILIPAR
                     PLDSSAFSTKGFNTSTPLVRSDSSASIFETESQERDILQWLKLFTFQLDVTKFIGQEA
                     SVVGFVYFDDKLEQNQFFVSRFVVSCCAADAFAIAMPVRWEKSAELQQDSWVVVRGTI
                     DSIDIEGVRTPMIIASAVEATDIPAQPYIYP"
     gene            complement(3211..4221)
                     /locus_tag="PLG52_05665"
     CDS             complement(3211..4221)
                     /locus_tag="PLG52_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNB40964.1"
                     /translation="MQTTTTNRLPRNAILFILLAVLIAVASSLMPDGFFTWLWDKLNV
                     FATVFLGIFVEAVPYLLLGTFGSGLVEVFVDRDQMSRWVSHRPAAAAISGAFMGMVFP
                     VCECGVVPLTRRLFKKGLPLSAGVSFLLAAPVLNPIVILSTASAFGWGQMLFWRFTIS
                     LVIAVVVGLVFSAQGDAAEVLRPVLAGEDHDHVHAEAGDSVGEKIRKALLITVDEFFD
                     MGRYLIIGSLLAAGLQTFIAQSSLLAIGSGPVLSVLIMLLLAVLLSICSTVDSFVALG
                     FMGTFSFGAVLSFLVFGPMVDIKSTIMYLQVFKRRAVAYIIGIPFLMSLLAGLIFNYF
                     VK"
     gene            4312..>4668
                     /locus_tag="PLG52_05670"
     CDS             4312..>4668
                     /locus_tag="PLG52_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40965.1"
                     /translation="MNYRHFKYEGWLYALAFLVALAVRFIALGALPLTDAEAAPALQA
                     LRISQSANPALDPHPFYILSTSLIFLMYGGGTNFLARLIPALIGSLLVLAPRLLDDRL
                     KPRPSLILAFFLALDPG"
BASE COUNT         1216 a         1151 c         1228 g         1073 t
ORIGIN      
        1 acccaagaat caacgcgccc gaaaggattt caagaaatgg aatgaccgca gttggcagaa
       61 aatacttgga ggcagccaaa gtaagaatgc ccaataaaaa tacagagccg gtatgcgtga
      121 gcgtaaccac cgttcccagc actacggcat gccatgatgt gccacgcgac ccgaccagat
      181 acgccgcgac caccgtctta ccgtgaccgg gagtcagcgc atggagggcg ccgagggcaa
      241 gtgcgataag taaggcaaat agataaaagg tcagcgagag ttctttgctg cgcacaaggt
      301 caagcagaat ttcttgtggc gaacgctgcg aaagttcagg gacgacttgt tcggcagttt
      361 cggtgacgac atcttttttc tgtccgaagg gcaaggcggg cgcgccactg tcccatgtgg
      421 tgagcaggcg actttgatcg tcgatgctgg cagggttgtg gatgtagtca atggttagga
      481 tgtgactgcg ttgagcgggg aagagaaagg cggtgccttc ttcggctgtg agataaaacc
      541 aattgataga cttcgccgct tccatgccgt tttcgatgac gatgcgttgt gtgttgttgg
      601 tactctggct taaatctgca gaaaggttgt agatgataaa ttcttcgccc gcttgaaacg
      661 cctgcataga ggatgggatg ttaatggagt caactgtcag cggaagagtt tttccatcga
      721 ggctgaccag gaacgagtcg aggcgtgagg cgctccaggt ttgggcttcg tcttcgctga
      781 cggtgccatc ctgatttgag tccatctcat tccaaataaa gttgacaagc agtggacctg
      841 gcttgatctg ccatttgatc gtcatctggg tttgggtgag gttgaccgtg atggtgtgag
      901 cgtacacatc tgctgggtgc gctttggcag tgcggagggg caataaaaga aaagcgatca
      961 tgaacatcgc cgtaaatttg aagagttgtt ttttcaatga gactccaatt aataaataac
     1021 cgtcaatgcg agggtgacct tgccttttgc cctcgcactg acgtgtgaga ttacggcaac
     1081 cactgcggca agataccttc ggcaatgacc tcagattgaa tggtgccttc ttgccaaaga
     1141 ccgatttctg gtctgtactg cccttttaat ttgaactgct gaaagagcaa ggcattgccc
     1201 caaggatccc attgcggaga gttataggtg tactctgggt cattggcgac gataatgcca
     1261 tcgagcaaat ttgggtcgaa gacccataac acttgggtgg gcttttcttc atcggcacgc
     1321 aagctgagga cggcgcgctc tgcaagcggt gaccatgcta cggaataata ggcataatca
     1381 taggtgtctt tttcgccgat gagagtaatg gtttcgttgg tggtcaggtc tgcgagtttg
     1441 atttgagtgc gcccgccttc ttcagtttgg gtgaaggtgg tgtagagaaa ttgattcgaa
     1501 tcaggcgacc atgttacagg tccgccagtg gtggagggga agaggaattg gtctcccgtt
     1561 ttaaagtcga tcatgtggat gaaatctgcc agcccgtcga aagaggcgag gcggtttgca
     1621 tccggcgacc aagatgggtt gtagcccaaa atactttggt cttcatagat agcttggtct
     1681 gcaccgcttt ccaaatccac aacccagatg cgcggtgaac caaacggcaa atcgggggtg
     1741 ggtcctgccg cttcacgcga gtaggcaatg cgtttatcgc ccggcgcaat gaccggcgtc
     1801 gtgcaacggt cgagtccgca atcgagcagc atggatgcgt cgccgccttc acggctcact
     1861 cgccacaagt caatgccgcc gttttgattt gcagaactaa agacgatgaa gtctccactt
     1921 tgagcgacgt cgaacgagat gaccttgatc gtttcatctg tgagacgctt tacattgctc
     1981 ccgagcttgg tcattgacca gatactgctc tgccctgcct ctgaaactac atatgccact
     2041 cgcggctcac gcacatgaaa ctgccaactt tgtgtttttt tcacttcgcg tgtggtagcg
     2101 ttgatctcac cggggctaac cttgaaggtg taagttacac cttgttcata gggtttaagt
     2161 ggaatgaaac gtacgctgta gtcatccaca gctgataaat acccttcgtg gatcggttca
     2221 agagagatta ggtctgatgc aatctctgca gagatttttt ctgggaaagt aaaagtcact
     2281 gcttcgaaag gaccgacgac accatcttct ggtaagtcca cgcgaacttg gataccagcg
     2341 ccttcaccta ttaaaatgac aatgccaata atgattgtaa gcgcaatcat cacactaacg
     2401 gcaagggtat caatcgaaag atgagttttc atgggtagat ataaggctga gcgggaatat
     2461 ctgttgcttc aacagcacta gcgataatca taggagtgcg taccccttcg atatcaatcg
     2521 aatcgatagt acctctgact accacccagg agtcttgctg caactctgca gacttctccc
     2581 agcggacggg catggcgata gcgaaagcat ctgctgcaca gcaggagaca acgaagcgac
     2641 tgacgaagaa ttggttttgc tctagtttgt catcgaaata aacgaaccca actacagagg
     2701 cttcttgtcc aataaactta gtgacgtcaa gttgaaatgt aaagagtttg agccattgca
     2761 agatgtcgcg ttcttgcgat tcggtctcga aaatgctggc ggatgaatct gacctgacca
     2821 gaggggtaga ggtgttgaag cccttggtgg aaaatgcaga tgaatccaat gggcgggcag
     2881 ggatgagaat accgatcaac agcggcacca acatcaccca caaggtgacc gaggatggcg
     2941 cgtggtcatg ttcgtgatga tgttcaggat gttcttcttc atgtttgcgc gagcgacggt
     3001 tttcactgaa gacggtttgc gcgagaatgc cgaggaagat catgccggca atggtcagcg
     3061 gaacgaagcg agagttgatg taccacgtca actgtccatt gacggcttta aacacaaaga
     3121 agatgaacaa cccgaaaagt agtaatcctt gatacgaacg gaacattcgt tgaggcataa
     3181 gttctccaaa agcgatgatg gacgcttgtc ctatttcaca aaataattaa aaatcaaccc
     3241 ggcgagcaaa ctcatgagga agggaattcc aatgatgtat gccacggcgc gacgtttgaa
     3301 aacttgcaga tacatgatgg tgcttttgat atccaccatg ggaccaaaca ctaaaaatga
     3361 aagcacagcc ccaaagctga atgtgcccat aaagccgagc gctacgaacg aatccactgt
     3421 cgaacaaata gaaagcagaa cggcaagcag aagcatgatc aagaccgaaa gaaccggtcc
     3481 acttccaata gcaagcaggg acgattgcgc aatgaaggtc tgcaagccag ctgcgagcag
     3541 cgatccgatg atgagataac gccccatgtc gaagaactca tccacagtaa taagcagcgc
     3601 cttgcggatt ttctcgccaa ccgaatcgcc cgcctccgca tggacgtggt catggtcttc
     3661 gccagccaac accggacgaa gcacttctgc cgcatcacct tgtgccgaga agaccaatcc
     3721 caccaccaca gcgatcacaa ggctgatggt gaatctccag aagagcattt gcccccaccc
     3781 aaatgcagag gcggtgctga gaatcacgat ggggttaaga accggtgccg ctagcaagaa
     3841 cgaaacaccg gcagaaagcg gaagcccttt cttaaagagg cggcgggtaa gtggcaccac
     3901 gccacattca caaaccggaa agaccatgcc catgaacgca ccgctgatcg ccgcggcggc
     3961 aggacgatga gatacccaac ggctcatctg gtcgcggtca acgaatactt ctaccaagcc
     4021 agagccaaag gttcctaaca agagataagg caccgcttcc acaaagatgc caaggaatac
     4081 cgtggcgaag acgttgagct tgtcccatag ccaagtgaag aagccatcgg gcatgaggct
     4141 ggatgcaaca gcgattagta cagccaaaag gatgaacaaa atcgcattgc gcggtaatcg
     4201 atttgtagtt gttgtttgca caggagagat tatactcacc gtcgcagcat caaaattaaa
     4261 accacttgag cgttctcctt tatccacccc aaacagatta caatagcgcc tatgaattat
     4321 cgtcatttca aatacgaagg ctggttgtac gcacttgcat tcctggttgc cctggcggtt
     4381 cggttcatcg ccctcggcgc cctgcccctt accgacgcag aggccgcgcc tgctttgcaa
     4441 gctttgcgca tttcgcaaag cgccaacccc gccctcgacc cacacccgtt ttacattctt
     4501 tccacttctt taatcttcct catgtatggc ggtgggacaa acttccttgc gcgactcatc
     4561 cccgctctga ttggaagtct gctcgtgttg gcgccccgcc ttttggatga ccgcctcaaa
     4621 ccccgcccaa gcctgatcct cgctttcttt cttgctcttg accctggc
//
LOCUS       DAOQNJ010000148         4666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11315_length_4666_cov_8.166558, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000148 DAOQNJ010000000
VERSION     DAOQNJ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11315_length_4666_cov_8.166558"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /gene="dnaG"
                     /locus_tag="PLG52_05675"
     CDS             <1..1771
                     /gene="dnaG"
                     /locus_tag="PLG52_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNB40966.1"
                     /translation="PAFVIWPESGTWRCFGACNEGGDIFKFVMKREGIDFKEALNKLA
                     LRAGVEVQQIQRETPQQREAYDHLRTLLEDAVVYYRSHLFANPEILNYLRQKRGLTDA
                     TIETFGLGYAPPGYDNLLKHFSGKYMPQDLVDAGMLSERDSGGLFDRFRHRIMIPIRD
                     ENGKMAGFGARIVDPNDNPKFLNSPETAIFTKGRLLYGFDRARKPIRAADQAVIVEGY
                     LDVIALHQAGYENVVSPMGTALTEDQLRLLKRSTRRIVLALDPDVAGQKAVLRGLEAA
                     RSAMDKEGELTFDAHGLLRNEARLQADLRVATMPDDLDPDEIVARNKEEWATLIANAK
                     PIITHVMEALSAGQNLNDPKIKNQIAEQVLPLIDDMGTPMERDTYRQALARMLRVDER
                     TLMAAQPKGAVMRRKPRVTEPSRRTESVVVAAVNPRKNIEFYCLSVLIRKPDLVYRLD
                     RKLEEFGLSPLATEDFEYTDHQLLFNVLRQAMGQDEKDHAQYVFSQIPEDLAPLVNEL
                     LAQTEKLESSDDKLLEDLLARFLDLRRFHAMSSVTQLKFMQDDEQQQGGENIKVYIEQ
                     TMRFTRLLNGLDQAKLKLSKRQA"
     gene            1801..>2825
                     /locus_tag="PLG52_05680"
     CDS             1801..>2825
                     /locus_tag="PLG52_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB40967.1"
                     /translation="MKPKRTSASKTTKKKGMEKEKKKAVKQTSPEKPRKGLRSGGSIG
                     DESMPEFVTDDFAEPDDTLFVPQEEELDESINIHAHLTGEPGEDPVRLYLREIGQVKL
                     LAADSEFRLATLIEGDRLISTLNKLKQRKGVSQAASTYHSVLSEALTSWDRLVEDTER
                     LEHDLPNLALLLAEAQSLHAGWEFDSPSYLRAYLDNGQWGSDHLWDNLARHAYSVFLS
                     LYLLPYPYAEWLLSHVRVHQGFPAQRTLFRNLPSDEELISEMESVHLRAIDAQQALVR
                     ANLRLVVSVAKRYLGRGIPLLDLIQEGNMGLLRAIGKFDPRRGFKFSTYATWWIRQSI
                     NRSIAEQA"
     assembly_gap    2826..2925
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2926..3569
                     /locus_tag="PLG52_05685"
     CDS             <2926..3569
                     /locus_tag="PLG52_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB40968.1"
                     /translation="YATWWIRQSINRSIAEQARTIRIPVHLFESISRILRAQRHLTQV
                     LGREPTNEDIALEVGYLSAVDVQSILRAHAESKPVHPELQRKLDVASQKVTRALRSAE
                     ETISLEGPVGDDESSALGDFIEDEDALSPMDAAAREMLREQITHALESLSDRERDVLE
                     LRFGLKDGKEHTLEEVSRFFDVTRERVRQIEAKALRKLRHPSKSRELRDYLGD"
     gene            3771..4127
                     /gene="ndhC"
                     /locus_tag="PLG52_05690"
     CDS             3771..4127
                     /gene="ndhC"
                     /locus_tag="PLG52_05690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNB40969.1"
                     /translation="MLLEYIAIGVMILVATLIAFIAIGLGELFGPKKKSAGKDTPYES
                     GMNPYGEGTRRMPVRFYLIAVLFILFDIEVVFFLPWAIAFRKLGLFGLIEMVVFITVL
                     LIGYVYAWKKGALEWE"
     gene            4118..4612
                     /locus_tag="PLG52_05695"
     CDS             4118..4612
                     /locus_tag="PLG52_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNB40970.1"
                     /translation="MGVEQKLGNMGVVTTTLENVVNWSRTNAMWPMLFGLACCAIEMM
                     AAQAAHYDMSRFGMELMRASPRQSDLMIVAGRVSKKMGPVLRRLYDQMPEPKWVIAMG
                     DCASCGGVFNNYAIYQGVDEVVPVDVYVAGCPPRPEALIHGVMTLYEKVNAMKLAEKP
                     AESK"
BASE COUNT         1125 a         1066 c         1195 g         1180 t
ORIGIN      
        1 gcctgcgttt gtgatctggc ccgaatcagg cacatggcgc tgttttgggg catgcaatga
       61 aggcggggat atcttcaagt tcgtgatgaa gcgtgaaggc atcgacttta aagaagcctt
      121 gaataaactt gcgcttcgag ccggtgtgga agttcaacag atccaacgcg agacgccgca
      181 acaacgtgaa gcgtatgatc atttgcggac tctgttggaa gatgccgttg tttattatcg
      241 cagtcattta tttgcaaacc ctgagatatt gaattatctg cgccaaaaac gcgggttgac
      301 ggacgctacc attgagacct ttggtttggg ttatgcccct ccaggctatg ataatctgct
      361 caaacatttt tcgggcaagt atatgccgca ggatcttgta gatgcaggca tgctctctga
      421 aagagatagc ggcggattgt ttgatcgttt tcgtcatcgc attatgattc ccatccgcga
      481 tgagaacggc aagatggcgg gttttggcgc acgcatcgta gacccgaacg ataaccccaa
      541 gtttttgaat tcacctgaaa cagcgatctt taccaagggg cgtttgttgt atggctttga
      601 ccgcgctcgt aagcccatcc gcgcggcaga ccaggctgtg atcgtggaag ggtatctcga
      661 tgtgatcgcc ttgcatcagg cgggttatga gaatgtcgtc tcgccgatgg gcacagcact
      721 gaccgaagac cagttgcgct tgctcaagcg ttctacccgc agaattgtcc tcgcgctcga
      781 cccagatgtg gcaggtcaga aagctgtgtt gcgcgggctt gaagcggctc gctctgccat
      841 ggataaagaa ggggagctga cctttgatgc acatggcttg ctccgcaacg aagcccgcct
      901 ccaagcggac ttgcgtgtag cgaccatgcc cgatgatctc gaccccgatg agattgtggc
      961 acgcaacaaa gaagaatggg ctacattgat tgcaaatgcc aagcccatca tcacacatgt
     1021 gatggaagcc ctttctgccg ggcagaattt gaacgacccc aagattaaga atcagatcgc
     1081 cgaacaagtg ttaccgctga tcgatgatat gggtacgccc atggaacgcg atacgtatcg
     1141 gcaggcgttg gcgagaatgt tacgggttga cgaacgcacc ttgatggctg cccagcccaa
     1201 aggcgcggtg atgaggcgca agccacgtgt cacggaaccc agccgcagaa cagagtctgt
     1261 tgtggttgcg gcagtgaatc caagaaaaaa tattgagttc tattgtttga gcgttttgat
     1321 tcgcaagccg gatctggtct atcgtttaga ccgtaagttg gaagagtttg gcttgagccc
     1381 attggcaaca gaagatttcg agtatactga ccatcagtta cttttcaatg tattgaggca
     1441 ggcgatggga caggacgaaa aagatcatgc tcagtatgtg ttctcgcaaa ttcctgaaga
     1501 ccttgcgccc cttgtcaatg aattgcttgc acaaacagaa aaacttgaat catcagacga
     1561 taaattgttg gaagatctct tagcacgctt tttagacctg cgacgtttcc atgccatgag
     1621 cagcgtcacg cagttgaagt tcatgcaaga tgatgaacaa caacaaggcg gagagaacat
     1681 caaggtctat attgaacaaa caatgcgctt tacccgttta ctaaacggtt tggatcaggc
     1741 gaagttaaaa ttgtcgaaac gacaggcgta attcaacccg ctttgtagga gacaggttcc
     1801 atgaaaccga aacgaacgtc tgccagcaaa acaacgaaga aaaagggcat ggaaaaagaa
     1861 aagaaaaagg cagtgaagca aacatcgccc gaaaagcccc gcaagggtct tcgttcgggt
     1921 ggctccatag gggatgaatc catgccagag tttgtcacgg atgattttgc cgagcccgat
     1981 gacacgctct ttgtgccaca agaagaagaa cttgatgaat ctataaatat acatgcgcat
     2041 ttaactggcg agccgggcga agacccagtg cgtttgtatt tgcgcgagat cggtcaggtc
     2101 aaattattgg cggcagatag cgagtttcgc cttgccactc tgatcgaagg tgaccgcctt
     2161 atttccactt tgaataaact caaacaacgt aaaggggttt cgcaggcggc gtccacttat
     2221 cattccgtac tttctgaagc gcttacttct tgggatcgat tggtggagga taccgagcgc
     2281 cttgaacatg acttgcccaa cttggcgctc ttgctggcag aggctcaatc gcttcatgcc
     2341 ggatgggaat ttgactctcc ttcctatctg cgtgcctacc tcgacaacgg tcagtggggg
     2401 agtgaccacc tatgggataa cctggcgcgt catgcttata gtgttttcct aagcctgtat
     2461 ctactaccat atccctatgc ggaatggttg ttgtcgcatg tgcgtgtgca tcaaggcttt
     2521 cccgctcagc ggaccttgtt ccgtaatttg ccatccgatg aagagttgat ttctgaaatg
     2581 gaatccgtcc atttgcgcgc catcgatgct caacaggcat tggtccgcgc taatttgcgt
     2641 ttggtagtga gcgttgccaa acgttatctg gggcgtggca ttccgttatt agacttgatc
     2701 caagaaggca atatggggct tctgcgtgcc attggcaagt ttgacccacg gcgcggattc
     2761 aaattcagca cctatgccac ttggtggatt cgtcagtcca ttaaccgttc catcgccgaa
     2821 caagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctat gccacttggt
     2941 ggattcgtca gtccattaac cgttccatcg ccgaacaagc gcgtaccatt cgcattcccg
     3001 tacatctctt tgagtctatt tcgcgcatct tgcgcgccca acgtcacctc acgcaggttt
     3061 tgggtcgtga acccacgaac gaagatatcg ctttggaagt tggctatctc tctgctgtgg
     3121 atgtgcaatc cattttgcgg gcgcatgccg agagcaagcc tgtccacccg gaattgcaac
     3181 gcaagttaga tgtcgcttcg caaaaggtca ctcgtgcttt gcgctctgcc gaagaaacca
     3241 tctcgcttga aggtccggtg ggggatgacg aatccagcgc tttgggcgat ttcattgaag
     3301 atgaagacgc gctttcgccc atggacgctg ccgcccgcga aatgctgcgt gagcaaatta
     3361 cgcacgcctt ggaatctctt tcagaccgtg agcgcgacgt cttggaattg cgttttggac
     3421 ttaaagatgg aaaagaacat accttggaag aggtcagtcg cttctttgat gtgacccgtg
     3481 aacgggtgcg ccaaattgaa gccaaagcac tccgcaaact tcggcaccca agtaagagcc
     3541 gagaattaag agattattta ggtgattaat aaaccaaagt gacagtttcc ataacgagac
     3601 tgtcactttt ttgttttctt ggagtgtttt ctatgaattc gattacattt tgcccaacat
     3661 gacaaaaatc atcatttttg tgtaatgtag cacaagtgct gggcttaaaa aaacctttgt
     3721 gatatacttt cgcgaactgt gcaagatttc acacatccat agaggtgttt atgctgctgg
     3781 aatatattgc cattggggtc atgatcttag tggccacgtt gatcgctttt attgcgatcg
     3841 gtctaggtga gctttttggt ccaaaaaaga aatctgcggg gaaagatacg ccctatgaat
     3901 cgggcatgaa tccatatggt gaaggaacgc gtcgtatgcc ggtgcgtttc tatttaatcg
     3961 ccgtattatt cattctgttt gatattgaag ttgtattctt tttaccttgg gcgatcgcat
     4021 tccgcaagct cggtctcttc ggcttgatcg agatggtggt tttcattacg gtccttctta
     4081 ttggttacgt ctatgcttgg aaaaaaggag ctttggaatg ggagtagaac aaaaactcgg
     4141 aaatatgggc gtggtaacca ccacgcttga aaatgttgtg aactggtcac gcaccaacgc
     4201 catgtggccc atgttgttcg gtttggcttg ctgtgccatt gagatgatgg ctgctcaagc
     4261 cgcccattat gatatgagcc gcttcggtat ggaactcatg cgtgccagcc ctcgtcagtc
     4321 tgatttgatg atcgtggctg ggcgtgtctc caaaaagatg ggacccgtcc ttcgccgttt
     4381 gtacgaccag atgcccgaac ccaagtgggt gatcgccatg ggtgattgcg cttcttgcgg
     4441 tggcgtcttc aataactatg ccatctatca aggtgtggat gaagttgtgc ccgtggacgt
     4501 gtatgtggct ggctgtcccc cgcgccctga agccttgatc cacggcgtga tgaccttgta
     4561 tgaaaaagtc aacgcgatga agctggcaga aaaacccgca gaatcgaaat aaaaaaaaca
     4621 gacctgacag atttcaagac ctgtcaggtc ttaggtgtat ttatgg
//
LOCUS       DAOQNJ010000149         4662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11338_length_4662_cov_9.402214, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000149 DAOQNJ010000000
VERSION     DAOQNJ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11338_length_4662_cov_9.402214"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..619)
                     /locus_tag="PLG52_05700"
     CDS             complement(128..619)
                     /locus_tag="PLG52_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40971.1"
                     /translation="MMPTINFNGKTYNSIEEMPANERQAFEKLSSLLVDANGNGIPDL
                     LEGDMVQNVMAVHATKTSINVNGQTYHSLEDLPPDLRQSVDGAFQFLSKMGIANTTSD
                     IQTPAMHQKPQQKSKPFLAQESPSAIEEENGRSTFSIVLISIVLCFAVVAATIAIFVF
                     INR"
     gene            803..1207
                     /locus_tag="PLG52_05705"
     CDS             803..1207
                     /locus_tag="PLG52_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNB40972.1"
                     /translation="MPTLKDIEGIGPVNAGKLNKAGVRGLNGLLKLGASRKGRQEIAK
                     ATGINAKVILEWVNRADLFRVKGIGGQYSDLLEAAGVDTAVELSKRQPEALLEAMKKA
                     NAKLNKVNQMPGLSNVKTWIKNAKSLKRVIEY"
     gene            complement(1255..2490)
                     /locus_tag="PLG52_05710"
     CDS             complement(1255..2490)
                     /locus_tag="PLG52_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNB40973.1"
                     /translation="MQTPWEYRFAHRTQRMGSSVIRELLKFTEQPDIISFAGGLPAPE
                     VFPVNEFQEACNRVLTEHGAQALQYSTTEGYRPLREMISRHTDRYKVSVTPENILITS
                     GSQQALDFLGRLFINRGDYIVVESPTYLGALQAWNAYGAQYISVPADENGMIVEELEK
                     ALRVGPKFIYVLPNFQNPSGSTLSLERRKRLVALADQYGVPIIEDDPYGQLRYDGEHI
                     PSVVYLDAQYRNDGDHEYTGNVIYLSTFSKLLAPGLRLAWVVAPPKVIQKLVMTKQAA
                     DLHTSSFNQQVAYEVGKGGFLDEHVKVIRATYKERRDVMLEMMDEMFPAEMRWTKPQG
                     GMFLWSMMPDGVDAADVLRIAIERKVAFVPGAAFHPNGGGDNTMRINFSYSSPEMIRE
                     GITRLGSTLKEVLKKNGQH"
     gene            complement(2931..3788)
                     /locus_tag="PLG52_05715"
     CDS             complement(2931..3788)
                     /locus_tag="PLG52_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB40974.1"
                     /translation="MPILALVLLFLSASMHALWNFLLKSAEEKYVAMGWQTILSGSLA
                     LLVMVFYSGLPPKEMWLFAVISMLLEAIYFILLCIAYSDHDFSLVYPIARGAAPALLV
                     LWTAIFLHEQLTTGGYIGLAMITLGMMVIGGTTLLQSKGEKVHLKGILTALSVALVIS
                     LYTFIDGKAVKSGPALPYGLSMFVMVPFVVTPYIANQYGWDSFGRVWQKNRNYLLIGG
                     VLGLIAYMLALFAYTFAPLSYSGAIREVSAVIGAFLGWRFLKEQMGGVRVLGSAIVFI
                     GVMVIAIFG"
     gene            complement(3793..>4662)
                     /locus_tag="PLG52_05720"
     CDS             complement(3793..>4662)
                     /locus_tag="PLG52_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB40975.1"
                     /translation="APMPSEWIETNQIKLHVVTDGPIDGTPVILLHGFPEFHYGWRKQ
                     VPALTEAGFYVITPDQRGYNLSEKPKGISSYKLESLAKDIIGLFDHYGIQKARLVGHD
                     WGAVVAWYVGIRYPERLEKLAILNVPHPDVMTHFLLHDSEQRKKSWYVYFFQLPFITE
                     WDLSRQNYRNMARMLVGSGLKSTFSRRDIEEYKKAWSQPGALSAMINWYRSIVRESLK
                     DLFRKRQPPRRIHVRTLMMWGMKDVALSHKMAQPSIDLCDDGKLIFFEHATHWVQHDE
                     ANEVNKNLIEFLR"
BASE COUNT         1252 a         1107 c         1078 g         1225 t
ORIGIN      
        1 tcattgcttt ttgagaggtg tacgatcatg tacacctcta atgctgttaa tagcaaggat
       61 gtgccaggga ttggcgcagt gatggcggag atcacgccgg cgataagggt gtaaatgagg
      121 acgagtttca tcggttgata aagacgaaaa tagcaatcgt ggcggcgaca acagcaaagc
      181 aaagtacaat gctgattaaa acgattgaga aggtgctgcg accgttttct tcttcaatcg
      241 cggagggaga ttcttgcgcc agaaacggct tggacttttg ttgcggtttc tgatgcattg
      301 ccggagtttg aatgtctgac gtcgtgtttg cgatacccat tttcgataag aattgaaaag
      361 cgccatctac actttgacgc aaatctggtg gcaagtcttc aagagagtga taggtttgtc
      421 cgttgacatt gatgctcgtt tttgtggcgt gaactgccat cacattctgc accatatcgc
      481 cttcaagcag gtcgggaatg ccattgccgt tcgcatccac aagtaagctg gataattttt
      541 caaaagcctg tctttcattg gcgggcattt cttctatgct attgtaggtc tttccattga
      601 aattaatagt gggcatcatg aactcctgcg tcttgtctct ccattttaca cgcagatatt
      661 accaaaccta aaagtaaagt ttgtatcgtc agtttttaga taatgtggaa agaacaataa
      721 aatcttttgt gcttaagaat ggataaagaa ttggtttttt agtaattaga tgttatagta
      781 ttgaataagg agataacaac atatgcctac actaaaagat attgaaggaa ttggtcccgt
      841 caatgctggc aaacttaaca aggctggtgt tcggggcttg aacgggcttt tgaaattggg
      901 agcttctcgc aagggacgcc aggaaattgc aaaagctact ggtattaatg cgaaagttat
      961 ccttgaatgg gttaaccgtg cagacctgtt tcgtgtaaag ggaattggtg gtcaatattc
     1021 cgatttgctc gaagctgctg gtgtggatac tgcggttgaa ctttccaaac gccagccaga
     1081 agccttgctt gaggcaatga agaaggcaaa tgctaaactg aacaaggtta atcaaatgcc
     1141 cggcttgagc aatgtcaaaa cttggatcaa gaacgcgaaa tcactcaaac gtgtgattga
     1201 gtactaaata aaaaaatccc gatgtgatga acaatcggga tttttttatt taacttagtg
     1261 ttgtccgttc ttcttgagaa cttctttcaa cgtagagccc agccttgtaa tcccttcgcg
     1321 gatcatctct ggtgacgaat aagagaagtt gattcgcatg gtgttgtctc caccgccgtt
     1381 agggtgaaag gcagcgcccg gcacaaatgc cactttgcgt tcaatggcaa ttcgcaatac
     1441 gtctgcggcg tccacaccat cgggcatcat gctccataag aacatgccgc cttgaggttt
     1501 tgtccagcgc atttcggctg ggaacatttc atccatcatt tcaagcatca catcgcggcg
     1561 ctctttgtac gtggcgcgaa tcacctttac atgttcatcc aagaatccgc ctttgcctac
     1621 ttcatatgcc acttgttgat taaaactgga tgtatgtaag tctgcggctt gtttggtcat
     1681 taccaatttt tggatgacct tgggtggtgc cactacccag gcaagcctca aaccgggggc
     1741 aagtaacttt gagaacgtac tcaagtaaat gacgttgcct gtgtattcat ggtcgccgtc
     1801 attacgatat tgcgcatcca gataaacaac cgaaggaata tgctcgccat cgtagcgtag
     1861 ttgaccatag ggatcatctt caatgatggg cacaccatat tgatcggcaa gggcaaccaa
     1921 acgttttctg cgttcgaggc tgagagtaga gccagaaggg ttctgaaagt ttggaagcac
     1981 gtaaatgaat ttcggtccga cccgaagcgc tttttccaat tcctctacga tcatgccgtt
     2041 ctcatccgca ggcaccgaaa tgtattgtgc gccataggca ttccaagctt gtaacgcgcc
     2101 gagatacgta ggcgactcca ccacaatgta atccccccgg ttgatgaaca acctcccaag
     2161 aaaatccaac gcttgttgtg aaccagaagt gatcaaaata ttttcaggtg tgactgaaac
     2221 tttataacgg tccgtatggc gagagatcat ctcgcgcaat gggcgatacc cctctgtggt
     2281 gctgtattgc aatgcttgtg cgccgtgttc ggtcaacaca cgattgcagg cttcttgaaa
     2341 ttcattcacc gggaaaacct cgggggcggg caaaccgccg gcaaacgaaa tgatatcggg
     2401 ctgctctgtg aacttcaaca attcacgaat aaccgagctt cccattctct gtgtacggtg
     2461 ggcgaagcga tactcccagg gtgtctgcat tcttactcct tagtgtagag ataaaaaaag
     2521 cctgacaggt acgcgggtac ccatcaggct tgctgactca tggcaatcga atgccgaaac
     2581 ttaatatagc cagaaatcca ttcatggcac ctttatctta cctatttttg gcagaggacg
     2641 caactgtttt ttgtaactgt cgatagcttg aaaagacgtg acaaattgca ggatgctccg
     2701 cctcttcacc tgtttttgcc tgtttctgtc cattggaatc tatctacact gtgcacaact
     2761 tctcttatca aaccctcaga aatttagtag ttttcaaccc ttgcgcgttt ttgccatttt
     2821 ggtaaaatag gtcttgcaac gtccagggtg cccccctcac cctggacgtt gcactttaat
     2881 ttccaaacaa aaagccgcca atgtaggcgg cttttttcat ttctctctgt ttagccaaag
     2941 attgcaatca ccatcacccc tataaacaca atcgcagacc caagcactcg caccccgccc
     3001 atttgctctt tcaagaagcg ccagcccaaa aacgccccaa tcactgcact cacctcgcga
     3061 atcgcacctg aataactgag cggtgcaaag gtgtaggcaa ataaagccaa catataggcg
     3121 attaatccca acacgccacc aatcagcaga taattgcgat ttttttgcca caccctgcca
     3181 aatgaatccc agccatattg gttggcaata tacggtgtga ctacgaacgg caccatcaca
     3241 aacatcgaca aaccatatgg tagtgcaggt ccgcttttga cggctttgcc atcaatgaaa
     3301 gtatagagtg aaataaccaa cgccacgctc aacgcagtga gaatgccttt gaggtgcacc
     3361 ttctcgcctt tgctttggag cagagtcgtc ccgccgatca ccatcatgcc aagcgtaatc
     3421 attgccagac caatatagcc gccggtggtt aattgctcgt gcaggaagat ggctgtccac
     3481 aagaccagta aagcgggggc agctccacga gcaatggggt agaccagcga aaaatcatgg
     3541 tcactgtatg cgatgcaaag caaaatgaag taaatggctt ccaaaagcat gctgataacg
     3601 gcaaataacc acatctcttt gggcggtaaa cctgaataga agaccatcac aagcaaagcc
     3661 agtgacccgc ttaagatggt ttgccacccc atggcaacat atttttcttc tgcgcttttg
     3721 agtagaaaat tccacagcgc atgcatggat gcagacagaa acagaagaac aagcgcaaga
     3781 atgggcattc cgttacctca ggaactcgat cagattttta ttcacttcat tggcttcatc
     3841 gtgttgaacc caatgcgtgg cgtgttcgaa aaaaatcaac ttgccgtcat cacacaggtc
     3901 aatggacggt tgtgccattt tatggctgag agctacatct ttcatgcccc acatcatcaa
     3961 ggtccgcaca tggatgcggc gcggaggctg ccttttgcgg aataagtctt tcaggctttc
     4021 tctcacaatc gaacgatacc agttgatcat ggcagataag gcaccgggct gtgaccatgc
     4081 ttttttgtat tcttcaatat cgcggcggct aaacgtgctc ttgagacctg acccaaccaa
     4141 catgcgcgcc atgttgcgat aattctgacg gcttaaatcc cactcggtga tgaaaggcag
     4201 ttgaaagaaa tacacatacc aagacttttt cctctgctca gagtcatgca acaaaaaatg
     4261 tgtcattacg tcagggtgtg gcacattgag gatggcgagc ttttccaagc gttctggata
     4321 gcgaatgcca acataccaag ccaccaccgc accccagtcg tgcccgacga ggcgggcttt
     4381 ttgaatccca taatgatcaa agagaccgat gatgtctttt gccagagact ccaacttgta
     4441 ggaagaaatc cctttgggtt tctctgagag gttgtacccg cgctgatctg gtgtgatgac
     4501 gtaaaagccc gcttccgtca acgctgggac ttgcttgcgc cacccataat gaaactcagg
     4561 gaagccatgc aataaaatga ccggcgtccc gtctatgggt ccgtcggtca cgacatgaag
     4621 ttttatttga ttggtttcga tccattcgct tggcattggc gc
//
LOCUS       DAOQNJ010000150         4654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11370_length_4654_cov_9.116765, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000150 DAOQNJ010000000
VERSION     DAOQNJ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11370_length_4654_cov_9.116765"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="PLG52_05725"
     CDS             complement(<1..1553)
                     /locus_tag="PLG52_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNB40976.1"
                     /translation="MLTSRASRLKYNFSQDFFRPDGHVVFSDISSARAFANQMSALRT
                     APVSASDLYVISLLDEAYHILLRNYYSRYTGVMGRAMASLQSTFGSKYDLTLNKFTEE
                     FPPKAVFRGETTAGVYLATKLPNFGDFGHGVRAAAIEEMLLINNANKNPALAPYMDLF
                     DENVMNGSAFKDFTSHLLEFFSTQPGLGNGAAGKTETLAELLRGPIEASPDSLEGQLK
                     FILDKWGYLLGNEFSNRILRGMDYLREDVIRKQGPADTFNADVPVPTFTGHEYAEFER
                     YSPDQEWMPRLVLIAKNTYVWLDQLSKKYEREIKNLNDIPDEELDQLAARGFTGLWLI
                     GLWERSRASQRIKQRMGQEDAVASAYSLHSYDIANDLGGWNALQDLRSRAWQRGIRLS
                     ADMVPNHMGIDSQWVIEHPDWFLSLPFSPYPSYSFKSENLSSDLRVGIYLEDHYYDKT
                     DAAVVFQRRDLQTGDLRYIYHGNDGTSFPWNDTAQLDYSNPVVREAVIQVILHVARNF
                     PVIRFDAAMT"
     gene            1607..3889
                     /locus_tag="PLG52_05730"
     CDS             1607..3889
                     /locus_tag="PLG52_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNB40977.1"
                     /translation="MSTSLAQVIVLLFIGVFYLPVIFFAIQRREEGQGAATWLVVLYA
                     LLAMVINIAEAIWQDRGDNSFLFQELQTYISFTLIIILMITIQVFLKRELWWAWLAYF
                     GVWVIGLALILTNALNLPDTLWTNGELILYRSRLGPAWVMLGWLILSIVMILNLFNAQ
                     HQSRQPLLRSRLGYWWAPVIFTLVNDILLFSGILIIGQPVRLLVAIGMAYVVGTHYVP
                     DLKNITRRAMIYVASTIGIVGFYIAGFLIIQALFGGNPSYSPLLAGAVVALFIALIFA
                     PLTDLVSRAINIWTKGDQYDASLTIHQYSESISNILDMDRLASIAIGIMLEAMQIERG
                     FLFLVDSERQADGRKTYKIRSARSPEERQLVAVELEEDGLIASYFIREQKPLLQYDLD
                     LLPAFRAASTVEKNWFTELRSEVYIPIFAKKQWIGLLAFGSKLSGNRYTREDLSVLSA
                     HANQTAVALENARLVDNLMRLNQELRQARRTLEKNNQELQRIDQAKSDFISIASHELR
                     TPLTVIKGYTEMLMDDPKLEKGQKSMMKGIHDGTIRLHEVMDSMFDIAQIDARSLKPH
                     LQPVDVGQLIHQVCLEHAKTVKEREQILTIKAQAIPLVKADSHLLEKLFHHLIRNAIK
                     FTPNNGWITITAKHIPAIINLPNGGIEIIVSDTGVGVDPNLREVIFSKFYQPGELGKH
                     STSKSRFKGNGAGLGLALSKGIIEAHGGRIWVESPGYDEVNFPGSQFHIILPLTKLEN
                     GEVQRTSEPLTIELGKAKEA"
     gene            complement(3933..>4654)
                     /locus_tag="PLG52_05735"
     CDS             complement(3933..>4654)
                     /locus_tag="PLG52_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40978.1"
                     /translation="VFGHEHRQTMTMLNYNHAYYQGLVEAQGFTKEVDFVSCYLPADK
                     FQMPERVKKISERVMQRGHLEIKTFKSKKDLLSWADRIGTAYNNAFVNNWEYYPLSQR
                     EIDFVVENIMTIADHRLIKIIVHEQDVVGFLFAFHDVSAAMQRAKGKLFPFGLLGILM
                     ELRRTNTVAVNGMGILPEFQGHGGNALLYYAMGDTLLNFGQFTHVEMTQVAETTEQMR
                     ADLKNLNGVEYKNNRVYRKLL"
BASE COUNT         1248 a         1148 c         1085 g         1173 t
ORIGIN      
        1 gtcatcgccg catcaaaacg gatgaccggg aagttgcgcg cgacatgcaa gataacctga
       61 atcaccgcct cacgaacgac cggattgctg taatctaatt gagccgtatc attccacggg
      121 aaggaggtgc cgtcattgcc gtggtagatg tagcgcaaat cgccggtttg caaatcgcgg
      181 cgttgaaaga ccactgcagc atcggtctta tcgtaataat ggtcttcaag gtaaattccc
      241 acacgcaggt cacttgaaag attttcagac ttgaacgaat aggaaggata gggcgaaaaa
      301 ggcagggaca aaaaccaatc tggatgttcg atcacccact gcgagtcgat gcccatgtgg
      361 tttggaacca tatcggcaga cagacgaatg ccgcgctgcc aagcacgtga gcgcaaatct
      421 tgcaacgcat tccaaccgcc aagatcattg gcgatatcat acgaatgaag agaatacgcc
      481 gaagcgacag catcttcctg acccatgcgc tgtttgatac gttgtgaggc gcggctgcgc
      541 tcccataagc caatgagcca taaaccggta aatccgcgag ctgcaagctg gtctaattct
      601 tcatctggga tgtcattgag attcttaatc tcacgttcat actttttgga aagttgatcg
      661 agccaaacat aggtattctt cgcgatcaga acgaggcggg gcatccattc ttggtctggg
      721 ctgtagcgtt caaactctgc atactcatgc ccggtaaagg ttgggacggg aacatctgca
      781 ttgaacgtat ctgctggacc ctgcttgcgg ataacatctt cgcgcaaata atccatgccg
      841 cgcaaaatac gatttgaaaa ctcattacca agcaaatacc cccatttatc gagaatgaat
      901 ttaagctgac cttccaatga gtctggggag gcttcaatgg gtccgcgcaa aagttcggca
      961 agggtttcag tcttccccgc tgcgccattg cccaaaccag gctgagtgga gaagaactct
     1021 aaaaggtgcg acgtgaaatc tttgaaggca gagccgttca tcacgttttc atcgaacaaa
     1081 tccatatagg gagcgagtgc cgggttcttg ttggcgttat tgatcaacaa catttcttcg
     1141 attgctgccg cacgaacacc atgcccaaaa tccccaaagt ttggcaactt ggttgccagg
     1201 tacacccctg ctgtcgtctc accgcggaag actgctttgg gcgggaattc ttcggtaaat
     1261 ttattcaggg taaggtcata tttcgagccg aatgtagact gcaaggaagc catcgcccgc
     1321 cccatcacac ccgtgtaacg gctgtaataa ttgcggagta ggatgtgata ggcttcgtcg
     1381 agcagagaaa tcacatagag gtcagaggca gaaacggggg cagtgcgaag cgcagacatc
     1441 tggtttgcaa atgcccgagc ggaggaaata tccgaaaaaa caacatgccc gtcggggcga
     1501 aaaaagtctt gcgaaaaatt gtatttgaga cgggaggcac gtgaagttag catacaataa
     1561 tgataaccga ttaataatga attagagtaa aatttaaacc atcatcatga gcacatcgct
     1621 agcccaagtc atcgtcctat tgttcattgg cgtgttctat ctgccggtca tcttttttgc
     1681 tatacagcgc cgggaggaag ggcaaggcgc agccacttgg ttggttgttt tgtatgcctt
     1741 actggcaatg gtgatcaaca ttgccgaagc catttggcaa gaccgcgggg ataactcttt
     1801 tcttttccaa gaacttcaaa cgtatatttc gttcaccctg atcatcattt tgatgatcac
     1861 gattcaagtc ttcttgaagc gcgaattatg gtgggcttgg ctggcgtatt ttggagtttg
     1921 ggtcattggg ctggcattga tccttacaaa tgctttaaac ctgccagata ctttatggac
     1981 aaatggtgaa ttgatcctgt atcgttcgcg gttgggtcct gcctgggtca tgttgggatg
     2041 gttaatttta tccatcgtga tgatcctaaa tttattcaat gcgcaacacc aaagccgcca
     2101 accgttgctc cgcagtcgtt tgggatattg gtgggcgccg gtcattttca ctctcgtcaa
     2161 cgacattctt cttttctctg gaattcttat cattgggcaa ccggtgcgct tgttggttgc
     2221 aattggcatg gcatatgtag taggcacaca ctacgtgcca gacctaaaga acatcactcg
     2281 ccgtgccatg atctatgttg ccagcacgat cggcattgta ggcttttaca ttgctggctt
     2341 tttgatcatc caagctttat tcggtggcaa cccaagttat agtccgctat tggcaggcgc
     2401 cgttgttgcg cttttcattg cgttgatctt cgcgccactc acagacttgg tctcacgcgc
     2461 catcaatatc tggacgaaag gcgatcaata cgacgccagc ctcaccattc accagtatag
     2521 tgaaagcatc agcaacatct tggacatgga tcgccttgcc agcattgcca ttggcattat
     2581 gctggaagcc atgcaaattg aacgcggctt cctattcctc gttgattctg aacgtcaggc
     2641 agatggtcgc aagacgtata aaattcgttc ggcgcgcagc cctgaagaac gacaattggt
     2701 cgctgtggaa ttggaagaag atgggcttat cgcttcgtat tttattcggg agcaaaaacc
     2761 gcttcttcaa tatgacctgg acctgttgcc ggcgtttcga gctgcttcta ccgttgagaa
     2821 aaattggttt acggaattac ggtcagaagt ttacattccc atttttgcca aaaaacaatg
     2881 gatcggtctg ctggcattcg gttcaaaatt aagcggcaat cgttacaccc gagaagacct
     2941 ttccgttctt agcgcacatg ccaaccagac agccgttgcg cttgaaaacg cccgcctcgt
     3001 ggacaatctc atgcgcttga accaagaatt acgtcaggcg cgccgtacgc ttgaaaagaa
     3061 caatcaagaa ttacaacgca ttgaccaggc aaaatctgat ttcatcagca ttgcatctca
     3121 tgaacttcgt acaccgctca ccgtcatcaa aggctatacc gaaatgctaa tggatgaccc
     3181 aaagctagaa aagggtcaga agtccatgat gaagggcatt cacgatggga cgatccgtct
     3241 ccacgaagtg atggactcga tgttcgacat cgctcagatc gatgcacgct cgctcaagcc
     3301 ccatcttcag cccgtggatg tgggtcaatt gatccatcag gtatgtctgg aacatgccaa
     3361 gaccgtcaaa gaacgcgaac agattctaac catcaaagcc caagccattc cgctcgtcaa
     3421 agcggactcg cacctcttgg aaaagctttt ccaccacttg atccgcaatg ccatcaagtt
     3481 cacgccgaac aatggatgga tcaccatcac cgccaaacac atcccagcca tcatcaacct
     3541 ccccaacggt ggcatcgaga tcatcgtcag cgatactggt gtgggcgtag acccgaactt
     3601 gcgcgaggta atcttctcga agttctacca gccaggcgaa ctgggcaagc attccactag
     3661 caaatcacgc ttcaagggta atggagccgg tttagggttg gctctctcaa aaggtattat
     3721 tgaagcgcat ggtggtcgta tctgggtgga aagccccggc tacgacgagg tcaacttccc
     3781 gggcagtcag ttccacatca tcctgccgtt gaccaagctt gaaaatggcg aagtgcaacg
     3841 cacaagtgaa cctctcacca tagaacttgg aaaagcaaaa gaagcataaa aaaagagcgg
     3901 agatttataa atctccgctc tttttatcag aattacagta atttgcgata cacacgatta
     3961 tttttatatt ccaccccatt caagttcttt aaatctgctc gcatttgttc agtggtctca
     4021 gccacttgtg tcatttcaac atgggtgaat tgaccgaagt ttaataatgt atctcccatc
     4081 gcataataca acaaggcatt cccaccatgc ccctgaaact ccggcaggat tcccataccg
     4141 ttcaccgcta cggtatttgt acggcgaagt tccatcaaaa tacccaacaa cccgaacgga
     4201 aagagcttgc ctttcgcacg ctgcatggct gcagaaacat catgaaacgc aaagagaaaa
     4261 ccaaccacat cctgttcatg gacaatgatc ttgatcaggc gatgatcggc gatagtcatg
     4321 atattttcca ccacaaaatc gatctcgcgt tgtgagagcg gataatattc ccagttattc
     4381 acaaaagcgt tgttgtaagc ggtgccaatt cgatccgccc aactcaacaa gtcctttttg
     4441 ctcttgaatg tttttatctc taaatgacct cgttgcataa cacgttcaga gatcttttta
     4501 actcgttcag gcatttggaa tttatccgca ggtaaataac aagagacaaa gtccacttct
     4561 tttgtgaagc cctgcgcttc aaccaatccc tgataataag catggttgta attgagcatg
     4621 gtcatggtct gacggtgttc atgcccgaaa acca
//
LOCUS       DAOQNJ010000151         4649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11389_length_4649_cov_7.868742, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000151 DAOQNJ010000000
VERSION     DAOQNJ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11389_length_4649_cov_7.868742"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLG52_05740"
     CDS             <1..317
                     /locus_tag="PLG52_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40979.1"
                     /translation="DQFEGTPPPPPEIEPLKKELTQSPSFHSGKIYFPLDALYFIKPI
                     RPWVLKFLPKDSHGWMNLDTPDQGPKFDYAIAAATKRTAHEFELLQKLLPPEMFTRFT
                     DR"
     gene            363..809
                     /locus_tag="PLG52_05745"
     CDS             363..809
                     /locus_tag="PLG52_05745"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNB40980.1"
                     /translation="MDTKAKLNEAMKESMKSGDELRKRTVRMVLAAIKQVEVDKRADL
                     DEMAVTALIQKEVKNRREALEEAKKANRSDLIADNEAEIKVLEEFLPKAMSADELRAL
                     VQAAITETGAAAPSDMGKVMKIVMPKVAGRAPNDAISSMVKELLNQ"
     gene            854..2965
                     /locus_tag="PLG52_05750"
     CDS             854..2965
                     /locus_tag="PLG52_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNB40981.1"
                     /translation="MTTRPPVPRRTRILQYLMIAVVALASFGMLISPLSLNPNQQTYD
                     IGEVTQSSIRSPRDIEYVSEIRTEEARKAAESAVQSVYSPPDPSITRAQIERLRTSLL
                     NISAIRDNKELTNEEMRQSLLSLSDLRLKDETIDYLLGISSTRWQSVQDEAVRVLEQV
                     MRRAIYEDRVEAAQSSISSSVSLTFNEQQTELVTELVSPFVIANSFFSQELTDAEKLR
                     AREAIQPIVQTYKAGETIVPAGEIITPADVEAFQQLGMIRPGQRWEDIAATAAIVLLC
                     AAFVPIYFYRKPRSVFLNDPKNLTIISLLFVVFLAGARLLAERTLAPYGYPIQAAGLL
                     LTALFSMEVGLVFAIPLAVLSAFGLPNSADLIPYYMFTSLIGLLALGPARRFWGFLRA
                     GIAISISGAAVLMAFRIPLITLDWLGILQYMGMIAFAGFSSASIALLLQYIFAQILGL
                     TTALQLLDISRPDFPLLQFFLRNAPGTYQHSLQVANLAEQAAEKISADPLLTRVGALF
                     HDIGKALNPNFFIENQVQGNLNAHNNANPEEVSATIIRHVTDGVQLAKKHRLPRRLHD
                     FILEHHGTLITHYQYNQAMEAVGGDISKVDIEKFRYPGPRPASRETALLMLADATEAR
                     ARAERPTNDEEIRALVTSVIQTVQKYNQLDDTLLTLRDLRLITDSFVTTLRGTYHPRI
                     QYPKAAVPDQDTTLLVTRKEK"
     gene            2962..3432
                     /gene="ybeY"
                     /locus_tag="PLG52_05755"
     CDS             2962..3432
                     /gene="ybeY"
                     /locus_tag="PLG52_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNB40982.1"
                     /translation="MIHIDNQMDFRDTALLERAALVTLESVTPLNPDLGPDSAHADMS
                     IVLTDDRQLHELNLDYLGVDAPTDVLSFPAGEVDPETEALYLGDLAISIPRATQQAQN
                     GGHPVEAEAQLLVVHGTLHLLGYDHGTDGEKAVMWAEQAKVLERLGLSRIKIQE"
     gene            3435..3821
                     /locus_tag="PLG52_05760"
     CDS             3435..3821
                     /locus_tag="PLG52_05760"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNB40983.1"
                     /translation="MKEFILSRIRSFRYAFQGWGYVLRTQRNAWIHSAIATAVFIVGL
                     WLQLPARDWAVIILTAALVFSAEFINTAIEAVVDLATEEHHPLAKIGKDVGAAAVLVA
                     ALAAVLIGLLILGPPLLQKIKSIYAF"
     gene            3865..>4649
                     /locus_tag="PLG52_05765"
     CDS             3865..>4649
                     /locus_tag="PLG52_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNB40984.1"
                     /translation="MALSFKFGTVGSPSGTPKKPGGTVGAIAFSKSIGLETLELGWVQ
                     SVRVSEETCALIKTTRDEHDFALSVHAPYFINLNAEKEEWPKSRKRLMDAAHYGYLAG
                     VTDIIFHPGSYFGNDPAEVLKVAIPRLEGCVKELQKNGDKVNLRPETMGKSAMLGSFE
                     DALAMSKAMDWVQPCIDFAHLHARPGDGSMNSYDEWSRLLEKYGKTLGAKALKQLHIH
                     LSGIEYGPKGEKNHLPLSEADLDLKALFKALHEFGCGGRILCE"
BASE COUNT         1132 a         1208 c         1125 g         1184 t
ORIGIN      
        1 ttgaccaatt cgaaggcacg cctccacctc cgcccgaaat tgaaccgcta aagaaagagc
       61 tgacccaaag cccaagcttt cactctggga aaatatactt ccctttggat gccttgtact
      121 ttatcaaacc tattcgaccg tgggtgttga agttccttcc caaagactcg cacggttgga
      181 tgaatctcga tacccccgat cagggaccga aatttgacta cgccattgcc gctgcaacca
      241 aacgcaccgc ccacgaattt gaattactac agaaactctt acctcctgaa atgtttacaa
      301 ggttcacaga ccgataacat cttaatattt ttgcgtcact caagaaaggt ataatcaaac
      361 ccatggatac aaaagcaaaa cttaacgaag caatgaaaga atcaatgaag agcggagatg
      421 aactccgtaa acgcactgtc cgtatggtat tggctgccat taaacaagtg gaagtagaca
      481 aacgcgctga tttggatgag atggcggtca cagccttgat ccagaaagag gtgaagaacc
      541 gacgtgaggc gttggaagag gcgaagaaag ccaatcgctc tgacctgatt gccgataatg
      601 aagcggaaat caaagtgtta gaagaattcc ttcctaaagc catgtctgcc gatgaattgc
      661 gagctttagt gcaagccgcc attaccgaaa cgggtgctgc cgcccccagt gatatgggca
      721 aggtcatgaa gatcgtcatg cccaaagttg ccgggcgcgc cccaaatgat gccatcagct
      781 cgatggtaaa agaactgctc aaccagtaaa aacctcgctg cagggaggcg cttgtctccc
      841 tgcagtcgtt cgtatgacca cacgcccccc cgttccacgc cgtactcgaa tattgcaata
      901 cctgatgatc gccgtcgttg cactggcttc attcggtatg ctcatttcgc ctttgagctt
      961 aaaccccaat cagcagactt atgatattgg tgaagtgact caatcttcga tacggtcgcc
     1021 ccgtgatatt gagtatgtga gtgagatccg cacggaagaa gctcgcaaag ccgctgagag
     1081 tgcggttcaa tcggtatata gcccgccaga cccgtccatc acccgcgcac agatcgaacg
     1141 tttgcgcact tctcttttga atatctctgc cattcgcgat aataaagaac tgaccaatga
     1201 agaaatgcgt cagagtttgt tatcactgag tgacttgcgt ctgaaagacg agacgatcga
     1261 ttacctgctg gggatttcaa gtacacgttg gcaatctgtt caagatgaag ccgtgcgcgt
     1321 tctggagcag gtgatgcgcc gtgccattta cgaagaccgt gttgaagcgg cacaatcgag
     1381 catctcttct tctgtaagcc tgacctttaa tgaacagcaa accgaactgg tgacagaatt
     1441 ggtttcgccg tttgtgatcg caaacagctt cttcagccag gaattgaccg acgctgagaa
     1501 gttgcgcgcg cgcgaagcca ttcaacccat tgtgcaaacc tacaaagcag gcgagaccat
     1561 cgtcccggca ggggagatca ttacaccggc agatgtggaa gcctttcaac aactaggtat
     1621 gattcgtccc ggtcagcgtt gggaggacat tgctgctacg gctgctatcg ttttgctgtg
     1681 cgccgctttt gttcccattt atttttatcg caaaccgcgt tctgtattcc tgaacgaccc
     1741 gaagaatctc acgatcatct ccctgctctt tgtggtcttt ttagcagggg ctcgtctgtt
     1801 agcagaacgg actcttgcgc cctatggcta tccaatccaa gcggctgggt tattgctcac
     1861 tgccttattt agcatggaag tgggcttggt ctttgccatt ccgttggcgg tcttatctgc
     1921 atttggtttg cccaactctg ccgatcttat tccctattac atgttcacat cgctgatcgg
     1981 actgctggcg ttgggtccgg cacgccgttt ctggggcttc ttgcgagcag gcattgccat
     2041 ttctatttca ggtgccgcgg tgctgatggc atttcgcatt ccgctcatca cattggattg
     2101 gcttggcatt cttcaataca tgggcatgat cgcctttgca ggcttctctt ctgcgagcat
     2161 cgcgcttttg ttgcaataca tctttgcgca aatcctcggg ctgaccactg ccttgcaatt
     2221 actagatatt tcacgccccg actttccgct tttacaattt ttcttgcgca atgcgcccgg
     2281 cacctatcaa catagtttgc aggttgctaa tttggcagag caagccgccg aaaaaatcag
     2341 cgcagatcct ttgctcactc gcgtaggcgc tttattccac gatattggca aggcgctcaa
     2401 cccgaacttc tttattgaga atcaagtgca gggcaatctc aacgcacata ataatgctaa
     2461 ccccgaagaa gtttctgcca ccatcattcg gcatgttact gatggtgtgc agcttgccaa
     2521 gaaacatcgc cttccgcgcc gcttgcatga tttcatcctt gaacatcatg gcacgctcat
     2581 cacacactat caatataacc aagccatgga agccgtaggt ggcgacatct ctaaagtgga
     2641 cattgaaaaa ttccgttatc ccggtccacg cccggcttcg cgcgaaacag ccttgctgat
     2701 gttggcagat gccaccgaag cccgtgcccg tgcagagcgc ccgaccaacg acgaagaaat
     2761 tcgcgcactc gttaccagcg tgattcagac cgtccaaaaa tataaccagc tcgacgatac
     2821 cctcctcact ttgcgcgact tgcgtctcat cactgattct ttcgtcacca ccttgcgcgg
     2881 cacatatcat ccgcgcattc aatatcccaa agccgccgta cccgatcagg atacgaccct
     2941 gttagttacg agaaaagaaa aatgattcat attgataacc aaatggattt ccgcgatacc
     3001 gcactgcttg aacgtgcggc gcttgtgact cttgaatctg tcacgcctct caacccggac
     3061 cttggaccgg actctgccca tgcagatatg tccatcgtcc taaccgatga ccgccaactt
     3121 cacgagctaa atctcgatta tctcggcgtg gatgccccca cagatgtgct ctcctttcct
     3181 gctggcgaag tggaccctga gaccgaagca ctctatctcg gtgacctggc tatctccatt
     3241 ccgcgcgcca cacaacaagc gcagaatggg gggcaccctg tagaagccga ggcgcagctg
     3301 cttgtggtgc atgggacgtt acacttgctt ggttatgacc acggcacgga tggagaaaaa
     3361 gccgtgatgt gggcagaaca agccaaagtt ttagaaagac tcggtttgtc tcgcatcaag
     3421 atacaggaat aatcgtgaag gaatttatcc tctcgcgcat ccgttcgttt cgatatgctt
     3481 ttcaaggctg gggctatgtt ctgcgaacgc aacgcaatgc atggattcat agcgctattg
     3541 ccacggctgt tttcatcgtt ggtttgtggc ttcaactccc ggcacgagat tgggcggtga
     3601 tcattcttac ggcggcattg gttttttctg ctgagttcat caataccgcc atcgaagccg
     3661 ttgtggacct tgctacagag gagcatcatc cgctggcaaa gatcggcaaa gatgttggcg
     3721 cggcggctgt gttagtggcg gcgttggcgg ctgttctcat tggcttgctc attttgggtc
     3781 cgccgttgtt gcaaaaaatc aaatccatat acgctttcta attgacgaat tactgttact
     3841 catttctatt ttctggagac tctcatggca ctctctttca aatttggcac agttggttca
     3901 ccctctggca ctcccaagaa acctggtggg acggtagggg caattgcttt tagtaaatcg
     3961 atcggtttgg aaacgctgga attgggctgg gtgcaatcgg tgcgggtgag cgaagagact
     4021 tgtgcattga tcaaaaccac aagagacgaa catgatttcg cattgagcgt ccatgcgcca
     4081 tatttcatca acttgaatgc agagaaggaa gaatggccca aatcgcgtaa acgcctcatg
     4141 gacgcggcgc attatggtta ccttgctggg gtaacagata ttatttttca ccccggctcg
     4201 tattttggca atgaccctgc cgaagtgttg aaggtggcaa ttccgcgttt ggaaggttgt
     4261 gtgaaagaat tgcaaaagaa cggtgataaa gtgaatttac gccctgaaac gatgggcaaa
     4321 tctgccatgc ttggttcttt tgaagatgcg cttgccatgt cgaaagctat ggactgggtt
     4381 cagccctgca ttgactttgc ccacctccat gcccgccccg gtgatggttc aatgaatagt
     4441 tacgatgaat ggtctcgctt gttagaaaaa tatggcaaga ccttgggtgc caaagccttg
     4501 aagcagttac acattcacct ctctggcatc gagtatggac caaaagggga gaagaatcac
     4561 cttccgctct ctgaagcgga ccttgacctg aaagccctct tcaaagcctt gcatgaattt
     4621 ggctgtgggg gacgtatcct ctgcgaaag
//
LOCUS       DAOQNJ010000152         4645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11409_length_4645_cov_8.498257, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000152 DAOQNJ010000000
VERSION     DAOQNJ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11409_length_4645_cov_8.498257"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLG52_05770"
     CDS             <1..182
                     /locus_tag="PLG52_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40985.1"
                     /translation="EFAADPITHIVHPDVPEKEHPGSITADALPTLQFLTTELSQNFI
                     ANVRQTFFYKYLAKR"
     gene            203..562
                     /locus_tag="PLG52_05775"
     CDS             203..562
                     /locus_tag="PLG52_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNB40986.1"
                     /translation="MPELPTLPDPQAFYAQVWNIVVRIPRGKVASYGQIAKMIRPPAG
                     VDAETYAEFGALWVSNAVAASPNEVPWQRVVNSKGQITERDGLEAKRHKLMLEDEGVL
                     FHDRGRIDMRKYGWNGK"
     gene            599..985
                     /locus_tag="PLG52_05780"
     CDS             599..985
                     /locus_tag="PLG52_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNB40987.1"
                     /translation="MSFSSPPNPQAYYEQVWAIARLIPHGKVATYGQIAKMIPPPNGV
                     ELEAYVAFGPRWVGGAMANCPDDVPWQRVINSQGKISERPGAERQRPLLEAEGVIFDA
                     KDRVDLKKFGWSGKDESDVPQQPSLF"
     gene            1024..1386
                     /locus_tag="PLG52_05785"
     CDS             1024..1386
                     /locus_tag="PLG52_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40988.1"
                     /translation="MQSPEKPSRLGTISFILAILVVVVWCIYLIIFAATTEGGLNFGM
                     DGETAGYAVVLGGSVIMMVLTILLTLSGIVTGALALRNKDSKRGLAIAGLVLNFLCFA
                     PYCLLLALMAFSAIPTGN"
     gene            1460..1774
                     /locus_tag="PLG52_05790"
     CDS             1460..1774
                     /locus_tag="PLG52_05790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNB40989.1"
                     /translation="MYIFQVHHYIKPEGIDSYKAVTLENAQKTRLEPGVLRFEFFQDA
                     VNPCHFSLFEVYADMAARDAHLQTEHFLKWKDVYLATQERKGDGHEFISVYPEEGEWK
                     KD"
     gene            complement(1818..2645)
                     /locus_tag="PLG52_05795"
     CDS             complement(1818..2645)
                     /locus_tag="PLG52_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNB40990.1"
                     /translation="MYKSIPEWMRTNPIPAFFLLTFVIAWMIWLPIGYLAPKYFLLAI
                     IPGAWAPTIVSVLLTWLTDGKSGVRQFLSRVIRWRVSFQWYLVVLFGIAVIAFAAQGL
                     NIWLFDGEPVKLNLPAGIPENGLLIALPIIFFVNIFFGGPLAEDIGWRGYALPKLREK
                     MNAFTASLLIGIVWVLWHLPFFWIPEGRIAVGGIPLLWFALLTTAWSVLFAWVYVNTE
                     SILMPVLFHAAVNTTLGTLGILGQANGSLTPVILNTVLTWLLVGVIVFFTGRDLKKK"
     gene            complement(2701..3315)
                     /locus_tag="PLG52_05800"
     CDS             complement(2701..3315)
                     /locus_tag="PLG52_05800"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNB40991.1"
                     /translation="MRRTKEEAAVTRATLLKAALTLFSTKGYAATSIDDITQAAKLTR
                     GALYHHFTNKADLYNTLIEDVSARGADIVQGAVAEGGTFLDILKRVFVRQLAYIEESK
                     EARAVMELALVKTGAHPELQSGRDRQIESGKLMVASLAQTMQIGIASGELREDIPPME
                     MARSFIAFQNGVIQLWLASPKSFSLKSRAAVFADVLIQGLKKTA"
     gene            complement(3358..4329)
                     /gene="holA"
                     /locus_tag="PLG52_05805"
     CDS             complement(3358..4329)
                     /gene="holA"
                     /locus_tag="PLG52_05805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNB40992.1"
                     /translation="MPTIFLLYGNDEFAITRKLKDFESDFTDPTTADMNTTRLEARSM
                     TENDLNNAVNAMPFLAPKRLVFIAYPSGKFTKPDARKKFQEFLEKAPDTSKVVLYDVV
                     EKPKDAEKHWLVKWAEKNKPQVRSQAFFLPQQRDMPGWIINEAKNQGGQIEPAAAARL
                     AEMSGVDTRQAGMEISKLLAYVNWERPVRGSDVEAVCIVTSQQSVFDFVDALSQGNAK
                     TAQRLLHRLLENEDPFSLWGMVVRQFRLLLQAREILDARGNKDDVARALAIHPYVAEK
                     TTGQAGRFSMEALEGIYHRLLRIDEQVKTSQVTLDLALDTLVVELAR"
     gene            complement(4392..>4645)
                     /locus_tag="PLG52_05810"
     CDS             complement(4392..>4645)
                     /locus_tag="PLG52_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40993.1"
                     /translation="TNMATTALLAGTFAWFPLRGTKSTLWRLMLVPFALLFALAGYQL
                     SKPLGPWLQKYVTTEANLGSLQIIDIVPVRKGVHRVITK"
BASE COUNT         1206 a         1191 c         1109 g         1139 t
ORIGIN      
        1 gtgaatttgc agcggacccg atcactcata tcgtccaccc cgatgtgcca gagaaggaac
       61 accccggctc catcactgcc gatgcactgc ccactctgca atttctgaca acggaactct
      121 cacaaaactt catcgccaat gttcgacaga cattcttcta taaatacttg gctaaacgct
      181 aaccatcaaa aaggatattc ttatgcccga actaccaacc ctcccagacc ctcaagcttt
      241 ctacgctcaa gtatggaaca ttgtcgtccg catcccacgc ggaaaagtcg cttcgtatgg
      301 gcagatcgcc aagatgatac gtccgcctgc tggtgtggat gcagaaacgt atgcagagtt
      361 tggcgcacta tgggtcagta acgccgtcgc tgccagcccg aatgaagttc cgtggcagcg
      421 tgtggtcaac tcaaaggggc agatcaccga acgggacggg ttagaggcga agcgtcataa
      481 actgatgctt gaggatgaag gtgtcctctt ccatgaccgt ggacgaatcg atatgcgaaa
      541 atacggctgg aacggaaaat aatgaagcgg taaaccataa accgagaaag aaattcttat
      601 gtccttctcc tctcctccca acccgcaagc ttattatgag caagtttggg cgatcgctcg
      661 cctcatccca catggaaaag ttgctaccta cgggcagatc gccaaaatga ttccaccgcc
      721 taatggcgtg gaacttgaag cgtatgttgc atttggtccg cgttgggtgg gtggtgccat
      781 ggcaaactgt ccagacgatg tgccttggca gcgcgtcatc aactcgcaag gcaagatcag
      841 cgaacgcccc ggcgcggaga ggcaacgtcc gttattggaa gcggaagggg ttatcttcga
      901 tgccaaagac agagtggatt tgaaaaagtt cggctggagc ggcaaagacg aaagcgatgt
      961 gccacagcaa ccatctttgt tttaacctat aatctgagaa ctaactttaa taaggagaca
     1021 cctatgcaat cccctgaaaa accatcccgc cttggaacca tttcttttat tcttgctatt
     1081 ttggtcgtgg tcgtttggtg tatttacctg attatcttcg ccgcaaccac cgagggcgga
     1141 ctcaacttcg gcatggatgg cgaaactgcc gggtatgccg ttgttttggg cggcagtgta
     1201 atcatgatgg ttcttaccat cttgctaacc ctttctggaa tcgttactgg cgcccttgcc
     1261 ttgaggaaca aagactccaa acgtggtctc gccattgccg ggttggtgct taattttcta
     1321 tgctttgctc cctattgtct tttattggca ctcatggcat ttagcgccat ccctacagga
     1381 aattaacctc ttgcaatgat attgcatagt ttgcctctat tcgtttctat taaagtagaa
     1441 acaacaatcg gaggctgcga tgtatatatt tcaagttcat cattacatta aacctgaagg
     1501 aattgactcc tacaaagctg tcacgctgga aaatgcacaa aagactcgct tagaacccgg
     1561 cgtattgcga tttgaatttt ttcaagatgc cgtcaacccc tgtcacttta gtctctttga
     1621 agtctatgcg gatatggctg cgcgagatgc acacctgcaa actgagcact tcctgaaatg
     1681 gaaagatgtc taccttgcta cgcaggagcg caaaggcgat gggcatgaat ttatttcggt
     1741 ctatccagaa gaaggggaat ggaaaaaaga ttaacacttg agccagcttt ttagaaagct
     1801 ggcttttttt acttcattta tttctttttc aaatctctgc cggtaaagaa tacgatcaca
     1861 cctactaaca gccatgtcaa tacagtgttc aagatgacag gagtcagact tccattagct
     1921 tgacccaata tgcccaatgt tccaagcgtt gtattgacgg cagcatggaa caagaccggc
     1981 ataagaatgc tttctgtgtt gacataaacc caggcaaaca gaacagacca cgccgtcgtc
     2041 agaagcgcga accatagaag cggaatcccg cccacagcaa tccttccttc cgggatccaa
     2101 aagaaaggca aatgccagag cacccacaca atgccgatca ataggcttgc tgtgaaggca
     2161 ttcattttct cgcgcagttt tggcaaagca tagccgcgcc agccgatatc ttctgccaat
     2221 ggtccgccga aaaaaatatt cacaaagaaa atgatgggca gggcaatcaa caatccattc
     2281 tcggggatac ctgcaggaag atttaactta accggttcgc catcgaatag ccagatgttc
     2341 agtccttgtg cagcaaaggc gatgactgca atgccaaaaa gaactaccag ataccattga
     2401 aaactaacgc gccagcggat gaccctgctc aagaattgac ggacgccact ctttccatct
     2461 gtgagccatg tcaacaatac agatacgatc gtcggcgccc atgcaccagg gatgatcgcc
     2521 agcaagaaat atttcggcgc gagatagcca atggggagcc agatcatcca tgcgataaca
     2581 aaggtcaaca aaaagaaggc agggatcggg ttcgtcctca tccattcggg aattgatttg
     2641 tacatggtat tctccttttt acttttacat actatttagt atgtaaattg attaaaaaaa
     2701 ttaggcggtc ttcttaagtc cctggatcaa cacatcggca aagactgccg ccctgctctt
     2761 caacgagaaa gatttcggcg aagcaagcca tagctgaata acaccattct gaaatgcaat
     2821 aaaagaacga gccatttcca tgggaggaat atcttcgcgt aactcaccac tggcaatgcc
     2881 gatctgcatg gtttgagcaa ggcttgcaac catcaacttg cctgattcaa tttgccgatc
     2941 tcgccccgat tgcagttcag gatgcgcccc cgttttgacc aatgccagtt ccatcacagc
     3001 gcgcgcttct ttgctctctt caatataagc aagctggcga acgaacaccc gcttcaagat
     3061 atcaaggaac gttccgcctt ctgccacagc accttgcaca atgtctgctc cgcgggcaga
     3121 gacatcttcg atcaacgtgt tgtaaagatc ggctttgttg gtgaagtgat gatacaacgc
     3181 accacgagtc aactttgcag cttgggtgat gtcatcaatc gacgtcgccg catagccctt
     3241 ggtgctaaaa agggttaggg cagctttcaa aagggtggcg cgggtgactg ccgcctcttc
     3301 ttttgtacgt ctcatatata catactaatc ggtatgtaat tgtatgtcaa gagagattta
     3361 tcgtgccagt tcaacaacca gggtatcgag agccaggtct aatgttacct ggcttgtttt
     3421 tacctgctca tcgatccgca gcagacgatg gtaaattccc tccaaggctt ccatcgaaaa
     3481 acgccctgcc tgcccggtgg tcttttcggc aacatatggg tggatcgcca acgcgcgcgc
     3541 cacatcatct ttattaccgc gcgcatctaa aatttcacgc gcctgaagca gcaaacgaaa
     3601 ttgacgcacc accatccccc ataacgagaa tggatcttca ttctctaaca aacgatgcag
     3661 taacctttgc gccgtcttgg cattgccttg ggataaggcg tcgacaaagt cgaacacgct
     3721 ttgctgggat gtgacgatac aaaccgcctc cacatcggac ccgcgaacgg gtcgctccca
     3781 gttgacatat gccaacagct ttgagatctc catgccagcc tgacgggtat ccacgccaga
     3841 catttcagcg aggcgcgctg ctgcagcagg ctcaatctgc ccaccctgat tcttcgcttc
     3901 gttgatgatc cacccgggca tgtcacgttg ttgcggcagg aaaaaagctt gcgacctgac
     3961 ttgcggcttg ttcttctcag cccatttcac cagccaatgt ttttcagcat ccttgggttt
     4021 ttccaccaca tcatacaaaa caactttgga agtatcaggg gctttttcta aaaattcctg
     4081 aaatttcttg cgggcatcgg gttttgtaaa cttacccgaa ggatacgcta tgaacaccaa
     4141 acgctttggc gcaaggaacg gcatggcatt gaccgcattg ttgaggtcgt tctctgtcat
     4201 ggagcgcgct tcgagacgag tcgtgttcat gtccgctgtg gtgggatccg tgaagtctga
     4261 ctcgaagtct ttgagcttgc gcgtgatggc aaattcgtcg ttgccgtaga ggaggaagat
     4321 ggtgggcatg ggtttgttcg taggggcgag gtctcctcgc ccaagggcgg caggacgccg
     4381 cccctacgtt tttatttcgt aatcactcga tgcacgccct tgcggacggg cacaatgtct
     4441 atgatctgca gggagccgag attcgcttcg gtggtgacgt atttctgcag ccacggtccg
     4501 agcggtttgc tgagttgata gcctgccagc gcaaagagca atgcgaacgg aacgagcatg
     4561 agacgccaca gcgtagactt ggtccctcga agcggaaacc aggcaaaagt cccggcaaga
     4621 agcgcagtgg tcgccatgtt ggttc
//
LOCUS       DAOQNJ010000153         4630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11472_length_4630_cov_7.897705, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000153 DAOQNJ010000000
VERSION     DAOQNJ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11472_length_4630_cov_7.897705"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLG52_05815"
     CDS             <1..180
                     /locus_tag="PLG52_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40994.1"
                     /translation="LDVTRLDAASKLSQNRDDESYANIISHLEARGDDISAEIAHEMR
                     DRRPSLTNPPQTDKI"
     gene            232..750
                     /locus_tag="PLG52_05820"
     CDS             232..750
                     /locus_tag="PLG52_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40995.1"
                     /translation="MTETNPSSEQNPQVTNLSQVDVETVNAQLVRMHQSSAGLVTSED
                     VDMTMSAVAQAKVTNLKLTDSGIAALHADTATVQNSVVGVAQTTELALNGVAGAVVAG
                     SVEFGNAYAGIVAGREVRAERIESLILLSPNVQGNVTTVMDTRGAIIAGLISGLFAGI
                     MLLLGRMLFGKK"
     gene            854..1498
                     /locus_tag="PLG52_05825"
     CDS             854..1498
                     /locus_tag="PLG52_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNB40996.1"
                     /translation="MEKVVLKATKRDVIGKQVKAMRREGKLPAVIYGRHLEPISIVLD
                     AHTAGLALSKVSASSLVTIDVEGKEYPALVRERQRDFIKGVLTHVDFLAVDMNEKIRT
                     SVGLAFVGVSGAVKDYNGILVHNLERLEVECLPGDLPERINVDISLLKQIGDIFRVKD
                     LAISEKVRILADEEEPIAVVTITKDEASAQSAAAGTGEATSPELSVERGKKDEE"
     gene            complement(1564..2379)
                     /locus_tag="PLG52_05830"
     CDS             complement(1564..2379)
                     /locus_tag="PLG52_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB40997.1"
                     /translation="MKVQNKVMVVTGGGNGMGRELVLNLLAKGARVAAIDINETALKE
                     TAQLAKEYKENIATFVLNITDKDAVDALPAKVIAKFGKVDGIINNAGIIQPFVRLVDL
                     DYSAIQRVLDVNLYGTIYMVKAFLPHLKERPEAHITNVSSMGGFLPVPGQTVYGASKA
                     AVKLLTEGLYAELLNTNVKVTLVYPGAIGTNIAANSGITVPNMPEADSKEASNFKQLA
                     PDKAAEIIVNAIENNRYSVLVGSDAKFMDFIYRLNPQNAAKFIYNQMKALLPS"
     gene            complement(2601..3200)
                     /locus_tag="PLG52_05835"
     CDS             complement(2601..3200)
                     /locus_tag="PLG52_05835"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNB40998.1"
                     /translation="MTEITEEYIWNKLNTTDRSRVETDGYAEIPIHPDVQLKCAAVLV
                     PLVFFQNEWHVLYTRRTDRVESHKGQVSFPGGACDEGETTPEETALREADEEIGLNPK
                     DVKVIGKLSRMVTISKFKVTPVVGIIPFPYAFKTSGAEVARVFTMPLLWLANRNNYWE
                     FSMPGSGRTLLAYHPYDGELLWGATARMTVNFLSLLDLL"
     gene            complement(3202..3744)
                     /locus_tag="PLG52_05840"
     CDS             complement(3202..3744)
                     /locus_tag="PLG52_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB40999.1"
                     /translation="MFRRARRLLRGAPVPAVPPALRNAHRLMESGNYAEAALAFYDLA
                     RKAEERFPERAPILYTEAGRAAVLNGDAKKGVTHFRSALTLLSTQQRYRRLQNLGERI
                     VAELRERGLNAEADEVASVLRNNAQVSAGKEAPAPQKRAVLPTHCPSCGAAVRPDEVE
                     WLDDVTAECDYCGSPVRAES"
     gene            complement(3746..4585)
                     /locus_tag="PLG52_05845"
     CDS             complement(3746..4585)
                     /locus_tag="PLG52_05845"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNB41000.1"
                     /translation="MKYSLNAREGDLAQLVGLTHKHFIFMLQSGGDFQSHRGVIKHDD
                     LIGKPWGSQIFSHTGAPFFLLQPSMSDILNELPRNTQILYPKDIGYILIQMGISEGQT
                     VLEAGTGSGSMTISMATAVGPNGRIVSYEKTPDTQNLAKKNLQRIGLASRVDFKLRDI
                     QEGFDETDADAFFLDVQNPFDYIQQVRATLKPGGFFGTLVPTYNQVEKVLYALKKHNF
                     AFVEVCELMLRYYKTNPARLRPTDRMVAHTGYLIFGRKIEPSEDPRGAALAKEIIGIE
                     EEY"
BASE COUNT         1099 a         1131 c         1197 g         1203 t
ORIGIN      
        1 ttggatgtaa cccgcctcga cgccgcctcg aaactgagcc aaaaccgcga cgatgagtcc
       61 tatgcaaaca ttatcagcca cctcgaagcc cgcggagatg atatttcagc cgagattgcg
      121 cacgaaatgc gggataggcg cccttccttg acgaaccccc ctcaaacgga taaaatctga
      181 gccgttattc aacctgcgcc tccgtgagga ggcgtatttt ctgccaaaat catgaccgag
      241 acaaatcctt cttctgagca aaacccgcag gtgaccaacc tctcccaagt ggacgtggaa
      301 acggtcaacg cgcagttggt tcgcatgcac caaagctcgg ctggtttggt cacttcagaa
      361 gatgtcgata tgaccatgag tgcggtagcg caggcgaagg tcaccaactt aaagttgacc
      421 gactctggca tagccgcttt gcatgccgac accgccactg ttcagaattc agtagtggga
      481 gtcgctcaaa cgacagagtt ggctttgaac ggtgtggcag gcgcggtcgt ggcaggaagc
      541 gtggaatttg gcaacgcgta tgcaggcatt gtggcagggc gtgaagtccg cgcagagcgc
      601 attgagtcgc tgattctgtt atcgcctaat gtgcagggaa atgtcacaac tgtgatggat
      661 actcgcggcg cgatcattgc tgggttgata agtggcttgt ttgcaggcat tatgcttttg
      721 ctcgggcgca tgttgttcgg caaaaaataa ttcggatgtt cagcgggaat gacccgcttt
      781 acagaaattc attttggggc gaaggtaaca cagcggagtt gctgtgtgag aggcgcccaa
      841 ggagttaggt aagatggaaa aagtagtatt gaaagcaacc aaacgtgatg tgatcggcaa
      901 gcaggtgaaa gccatgcgcc gcgaagggaa actccctgcg gtgatttatg gtcgtcatct
      961 cgaaccgatt tcaattgttt tggatgcaca caccgccggc ttggctttgt cgaaggtttc
     1021 tgcttccagc ctcgtgacga ttgatgtgga aggcaaggaa taccctgctc tcgtccgcga
     1081 acgccagcgt gactttatca aaggtgttct cacccatgta gacttcctcg ccgtggacat
     1141 gaacgaaaag atccgcacca gcgtgggact cgctttcgtg ggtgtctccg gcgccgtgaa
     1201 ggattacaac ggtatcctcg tccacaactt ggaacgcctc gaagtggaat gtttaccagg
     1261 tgatcttcct gaacgcatca atgtagatat ctctctgctc aagcagatcg gcgatatctt
     1321 ccgcgtgaaa gacctcgcga tctccgaaaa agttcgcatc ttggcggatg aagaagaacc
     1381 cattgccgtg gtgaccatca ccaaggatga ggcttctgcc cagtctgctg ctgccggcac
     1441 cggcgaagcg acgtctcccg agctcagcgt ggaacgtggc aagaaagacg aagaataatt
     1501 tcttctgtac tagcaataaa aaagtccgcc atcgcaagat ggcggacttt ttttaccaat
     1561 gtattacgaa ggcagcaagg ctttcatttg gttatagata aacttggctg cattttgcgg
     1621 attcagtctg tagatgaaat ccatgaactt tgcatctgag cctacgagaa cgctgtagcg
     1681 attattctcg atggcattca cgatgatttc tgccgctttg tctggcgcta actgcttaaa
     1741 gttactggct tctttactgt ccgcttctgg catgttcggt actgtgatgc ccgaattggc
     1801 agcgatgttt gtaccaatgg cgcccgggta caccaaggtc acttttacgt tggtgttcag
     1861 caattctgca tataaacctt cggttagcaa cttaactgca gccttgctcg cgccataaac
     1921 cgtttgccca gggaccggca agaacccgcc catgctggaa acgttggtga tgtgagcttc
     1981 agggcgttcc ttcaaatgcg gtagaaaagc tttgaccatg tagatggtgc cgtacaggtt
     2041 tacatccaat acacgttgaa tggcggaata gtctaagtca acgagtcgca cgaaaggctg
     2101 aataatgccc gcgttgttga tgatgccatc tacttttccg aatttggcaa tcactttggc
     2161 aggcaacgca tcgacagcgt ctttatctgt aatgttcaag acgaatgtcg ctatattttc
     2221 tttgtactct ttcgccaatt gagcggtttc tttcaaagca gtttcgttga tatcgatggc
     2281 agccacacgt gcgccttttg ctaaaaggtt caacaccaac tcacgtccca taccgtttcc
     2341 gccgcccgtt actaccataa ctttattttg tactttcatg ttgatctctc ctttatcaat
     2401 ccttgggttg ctttcatctc cataagggga ggggcaccga gattgttgtt tgatcgttct
     2461 gatcgtctgt attgtaggtc tgagggagag attttgttag tgaaattaat cacaaataaa
     2521 gaggattatt gtgatatttt tcacaatata gagggtggaa attgtcctaa atctaacttc
     2581 aagttatata tctggagaaa ctacagcaaa tctaagagag acaagaagtt gaccgtcatg
     2641 cgtgctgtgg cgccccagag aagttcaccg tcatatgggt gataggcgag caaagtccgc
     2701 ccagagccgg gcatggaaaa ttcccaatag ttattgcggt tggcgagcca aagcaaaggc
     2761 atggtaaaga cccgcgccac ttctgcacca gaggtcttaa acgcataagg aaaaggaatg
     2821 atcccaacca caggggtcac tttgaattta ctgatcgtca ccatgcggct gagtttgccg
     2881 atcactttta catctttagg attcaacccg atctcttcat cggcttcgcg tagagcggtc
     2941 tcttcgggcg tggtttcgcc ttcatcgcaa gcaccgccgg gaaaagagac ttgccctttg
     3001 tgcgattcaa cccggtctgt gcggcgggtg tatagcacat gccattcatt ttggaagaat
     3061 accaacggaa ccagaaccgc cgcgcatttc aactgcacat ctggatgaat cggaatctcg
     3121 gcatacccgt ccgtttccac gcgcgaacgg tcggtcgtat tgagcttatt ccaaatgtat
     3181 tcttctgtaa tctcggtcat actaagattc agcgcgcacc gggctgccgc agtaatcaca
     3241 ttctgcggtc acatcatcca gccattcgac ttcatccggg cggactgccg caccacacga
     3301 tgggcaatgg gttggcaaga ccgctcgctt ttgcggtgct ggcgcttctt tccccgccga
     3361 aacttgggca ttgttcctga gaacggatgc tacttcgtca gcttcggcat tcaagccgcg
     3421 ttcacgtaat tccgctacaa tgcgctcgcc aaggttctgc aaacggcgat aacgttgttg
     3481 tgtgcttagt aaggtcagcg cactgcgaaa atgggtaacg cctttttttg cgtcgccatt
     3541 caaaaccgca gcacgacccg cttctgtata aaggatcggt gctcgttcag ggaagcgttc
     3601 ttcagctttt ctggcgaggt cataaaaagc aagtgcagct tctgcataat tccctgattc
     3661 cattaagcga tgggcatttc gtaaggcagg tggcaccgcc gggacgggcg ctccgcgtaa
     3721 taatcttctg gcgcgtctaa acatcttagt actcctcttc gataccgata atttctttgg
     3781 cgagtgctgc tccgcgtggg tcttcgcttg gttcaatttt tcgtccaaaa ataaggtagc
     3841 ccgtgtgcgc caccatacgg tctgtgggac gtaagcgggc agggttggtc ttgtaatagc
     3901 gtagcatcag ttcacagact tccacaaaag cgaagttatg ttttttcagg gcatacaata
     3961 ctttttcgac ctgattatag gtaggaacga gcgttccaaa gaatccgccc ggcttgagcg
     4021 tggcgcggac ctgttgaatg tagtcgaaag gattttgcac gtcgaggaag aaggcatcgg
     4081 catccgtttc gtcgaagccc tcttggatgt cacgcagctt gaagtccacg cgagaggcga
     4141 gaccgatccg ttgtagattt ttcttcgcca ggttttgcgt atctggcgtt ttctcatacg
     4201 aaacgattct tccgtttgga ccaactgctg ttgccatgga tatcgtcatc gagccagaac
     4261 ctgtgccagc ttcgagtacg gtttgccctt cagagatgcc catttggatg aggatataac
     4321 cgatgtcttt ggggtacaga atttgcgtat tgcgcggcaa ttcattgaga atgtcggaca
     4381 tggaaggctg aagcaggaag aagggcgctc ctgtatggct gaagatctgc gagccccacg
     4441 gtttgccaat tagatcatca tgcttgatca caccacggtg gctttgaaag tcgccacctg
     4501 attgcagcat aaagatgaag tgtttgtggg ttagccccac caattgcgcg aggtcgccct
     4561 cacgcgcgtt aagagaatat ttcatgcagc gaattctacc tttactttcg atttattcag
     4621 aaggggtttc
//
LOCUS       DAOQNJ010000154         4626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11485_length_4626_cov_6.887114, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000154 DAOQNJ010000000
VERSION     DAOQNJ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11485_length_4626_cov_6.887114"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PLG52_05850"
     CDS             <1..874
                     /locus_tag="PLG52_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNB41001.1"
                     /translation="VDLSLSVSDHHNQDGLRVLDAVRLLDPNCRAILLTGFATVEVAV
                     KALTEYNAFTFLRKESFKRSEFKDVIQRILVSAPTVDEPHASSADLKLDSASQTSAPL
                     KALIVDDDAGWRSILEDLLSEAEFQPVPCASFGEALGLIRKDKFSLAIVDLSLRDTQS
                     LDGLQLLKITQGHHIPTIVVSGLSEPEEIQRIYAEHSISTYIEKQAFDRNAFKRILAE
                     TRELSTTMDELSILTDREREVLDLLAQGLTNKEIAEKLVITTNTVKRHLKAVFEKLDV
                     HTRAAAVAIATKSK"
     gene            complement(933..2150)
                     /gene="fahA"
                     /locus_tag="PLG52_05855"
     CDS             complement(933..2150)
                     /gene="fahA"
                     /locus_tag="PLG52_05855"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNB41002.1"
                     /translation="MPKIQVDLNSDFPIQNLPYGIFSTKENPTPRVGTRLGDVVIDLA
                     MLDAENAFGKQYSFFADASLNPFMAAGRDVWREIRQRLTRLVAEEQASLKSDALIFIH
                     EVRMHLPVEVGDYTDFYASREHATNVGIMFRGKENALMPNWLHLPVGYHGRASSVVLS
                     GTDVIRPRGQVKPKDAPLDFVASRQLDFELEMGFFVGKGNDLGEPISIEDAHEHIFGM
                     VLLNDWSARDIQAWEYQPLGPFLAKNFATSISPWVVTMDALEPFRVKGPLQDPTPLPY
                     LGSNANGYDIQLEVSLQSARMDAPQVISHSNMKHLYWSIDQMLAHHTITGCNMRVGDL
                     CGTGTISGPTEDSYGSLLELTWRGERPIHLSSGEERMFLQDGDTLMMKGYCQGDGYRI
                     GFGEVTGKILPAK"
     gene            complement(2185..3120)
                     /locus_tag="PLG52_05860"
     CDS             complement(2185..3120)
                     /locus_tag="PLG52_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41003.1"
                     /translation="MNSPKIAFRTFMSQILDYAGMYPPANLSLDEAFKNFVAYQSDPY
                     AWMLSRFVIPAKRLKEISTFNEKISFTTLGRGGKDMDEFLANVNLDIADIRTFCDSHQ
                     AATVDMYEVALPASSLTDKFAANDIVTRAADALNKNGIMVFFEAPFVATGESWEVRAE
                     KLLRALRKVKDKHVGFKLRTGGVTADAFPSTAQVAWAMAQTREAGVPLKCTAGLHHPV
                     RHFNQSVQTKMHGFLNVFGAGILTAANGISQAEIQAILEDENAENFVFDENGFSWNTL
                     RVDNSEIKKARLFATSFGSCSFDEPKEDLQAIKLL"
     gene            3153..4067
                     /locus_tag="PLG52_05865"
     CDS             3153..4067
                     /locus_tag="PLG52_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNB41004.1"
                     /translation="MPHMYILSKDADEYRSILEQSKLPDLIIDKELTETCEILFGSPF
                     SIRDVLGKLPNLRWVQSTWAGVEPLLDPSLRRDYTLTNARGVFGGLMSEYIFGYLLAH
                     ERKIFERMEAQKNKQWDTSLTGSLRGKTIGLLGVGSIGAEVAQTAKVFGMTVRGYTRT
                     SKASAQVDRYYHGDELLEFVNGLDYLVNILPNTKETKKIIDANTLNALPSHAIFINVG
                     RGSTVDEPALLKVLQENKIAGAVLDVFEQEPLPSEHPFWDAPNLSITFHTSAPSLPED
                     IAQVFIENYKLYSDRKPLKHQVDFERGY"
     gene            4067..>4626
                     /locus_tag="PLG52_05870"
     CDS             4067..>4626
                     /locus_tag="PLG52_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNB41005.1"
                     /translation="MTVTINDIRQAAQRIKPYIHHTPVMTNESLNQRVGAQVFMKCEN
                     LQKVGAFKFRGASNAVWSLTDIEASSGVVTHSSGNHAQALALAAKIRGIPAYIVMPSN
                     APQVKKNAVAGYGGQITFCEPTLAARESTMESIRQRTGATVVHPYDNEHVIAGQGTAV
                     LELLEDIPDLDVIIAPVGGGGLLSGTS"
BASE COUNT         1319 a         1124 c         1024 g         1159 t
ORIGIN      
        1 tgtagacctt tccctctctg taagcgacca tcacaatcaa gatggtttgc gcgtgttaga
       61 tgccgtgcgc ttactcgacc caaattgtcg cgcgatccta ctcacagggt ttgctacggt
      121 ggaagtcgcc gtaaaggcac taaccgaata caacgccttt acctttctac gcaaagaaag
      181 tttcaagcgc agtgagttca aggacgtcat tcagcggatt ttggtcagcg cccccaccgt
      241 ggatgaacca cacgcttctt ctgcggactt gaaactggat tctgcttctc aaacctctgc
      301 gcccctgaaa gcgttgatcg tggatgatga tgccggttgg cgtagcattc tggaagacct
      361 gctcagtgaa gcagaatttc agcccgtgcc ttgtgccagc tttggcgaag cgctagggtt
      421 gatccgcaaa gataaattct cacttgccat tgtagacctt tccctgcgcg atacgcaaag
      481 cctcgacggg ctgcaattgc tcaagattac ccaagggcat catattccca ctatcgttgt
      541 cagtgggctt tccgaacctg aagaaattca gcggatttac gccgaacatt cgatttctac
      601 ttacatcgaa aaacaagcct ttgaccgaaa tgctttcaaa cgcatcctcg ccgaaacccg
      661 cgaactgagc accaccatgg acgagctaag catcctcaca gatcgcgaac gcgaagtact
      721 agacctgctc gctcaagggt tgaccaacaa agagattgct gaaaaactgg tcatcacaac
      781 caacactgta aaacgccact tgaaagccgt gtttgaaaag ctagatgtcc acacccgtgc
      841 tgctgcggta gccattgcaa caaaaagtaa ataaggacta ttaaccgcga agctcgccaa
      901 gtttttcttg gcgagcttcg cggttaaagg gtttactttg ctggaagaat cttccccgtc
      961 acttcgccaa agccaatacg atacccatct ccctgacaat agcctttcat catcaacgta
     1021 tcgccatctt gtaaaaacat ccgctcttct ccgcttgata aatgaatcgg tctctcgccg
     1081 cgccaagtta actcaagcag tgagccgtag ctatcttctg tcggtccgct aatcgtgccg
     1141 gtaccgcata aatcgcccac acgcatattg caccccgtga tggtgtgatg tgcaagcatc
     1201 tggtcgatgc tccaatacaa atgcttcata ttcgaatgac tgatgacttg cggcgcatcc
     1261 attcgagctg attgcaaaga cacctccaac tgaatatcgt atccgtttgc attcgagcca
     1321 agatacggca acggggttgg gtcttgcagt ggtcctttca cgcggaatgg ttccaaagcg
     1381 tccatcgtta ctacccaagg tgaaatagat gtagcaaaat tcttggcgag gaatggtcct
     1441 aaaggctgat attcccacgc ctgaatatca cgcgcgctcc agtcattgag caacaccatg
     1501 ccaaagatat gctcatgagc atcttcaatt gaaataggct caccaagatc atttcccttc
     1561 cccacaaaaa agcccatctc caattcaaaa tccaactgac gcgatgcaac gaaatccaac
     1621 ggcgcatctt tcggtttcac ctgtccacgc ggacgaatca catccgtacc cgaaagcacc
     1681 accgaactag cgcgcccgtg gtacccaact ggtaaatgca accaattcgg cataagcgca
     1741 ttttcttttc cacggaacat aatccccaca ttcgtagcat gttcgcgaga tgcgtaaaaa
     1801 tcagtgtaat caccaacctc aacgggtaaa tgcatacgta cttcgtggat gaaaatcaac
     1861 gcatcactct taagggatgc ctgctcttcc gccactagcc tagtcaaacg ctgacgaatt
     1921 tcccgccaca catctcgtcc tgccgccatg aaggggttaa gcgatgcatc tgcaaagaag
     1981 ctatattgct taccaaaggc attttcagca tccaacatgg caagatcaat gacaacatca
     2041 cctaaccgtg tacccacacg tggagtagga ttctcttttg tggagaatat tccataaggc
     2101 agattttgaa ttgggaaatc agaatttaga tcgacttgaa ttttcggcat agtttcacct
     2161 tataaatgag gttgttaact gaaactacaa aagttttata gcttgcaaat cttctttagg
     2221 ctcatcgaaa ctgcaactac caaaagacgt ggcaaacaat ctcgccttct ttatctctga
     2281 gttgtctaca cgcaaagtat tccaagaaaa tccattttca tcaaagacaa agttttcagc
     2341 gttttcatct tccaaaatag cttgaatctc tgcctgtgag attccgttcg cagccgtcaa
     2401 tatccctgcc ccgaaaacat tcagaaagcc atgcattttg gtttgcacac tttgattgaa
     2461 atgtcgaaca ggatgatgca gcccagccgt acacttgagc ggaacacccg cctcacgggt
     2521 ttgagccatc gcccatgcaa cttgcgcagt agatgggaat gcgtccgctg tcacaccgcc
     2581 ggtgcgaagt ttaaagccaa catgtttatc ttttactttg cgcaaggcac gtagcaattt
     2641 ctccgctcgt acttcccagc tttctccagt cgcaacaaaa ggagcctcaa aaaagaccat
     2701 gatgccgttc ttgtttaagg catcggcggc gcgagtaaca atatcgttgg ctgcaaattt
     2761 atcggtcaag gaagaagcgg gcaaggctac ttcgtacatg tctaccgtag cggcttggtg
     2821 agagtcacaa aatgtgcgga tgtctgcaat atccaagttc acattggcaa gaaattcgtc
     2881 catatccttg cctccacgcc ctagggttgt aaacgatatt ttttcattga aggttgaaat
     2941 ctccttcaag cgtttggcgg gaatcacgaa ccgtgacagc atccacgcat aaggatcgga
     3001 ttgataagca acaaaattct tgaaagcttc atctagcgac aaatttgcag gcgggtacat
     3061 tccggcataa tccaaaattt gcgacatgaa tgtacggaaa gctattttcg gagaattcat
     3121 atctgtatta tagcgggtaa aattggggca ctatgccaca catgtatatt cttagcaaag
     3181 atgcagatga atatcgatca atattagaac agtcaaaact acctgactta ataatcgata
     3241 aagagcttac tgaaacctgc gagattcttt ttggctctcc attctctatt cgtgatgtgt
     3301 tggggaaact tccaaacttg cgttgggtgc aatcaacatg ggcaggtgta gagcctctgt
     3361 tagatccatc tcttcggagg gattacacat taaccaatgc gagaggtgtg tttggcggat
     3421 taatgtctga atacattttt ggatatcttc tagcacacga gcggaagatc tttgagcgaa
     3481 tggaagcaca aaaaaacaag caatgggaca catccctcac tggttcgttg cgtggaaaaa
     3541 cgattgggct attaggggtt ggctcaattg gcgcagaagt agcgcaaact gcaaaagtct
     3601 ttggcatgac tgtgcgcgga tatacacgaa ctagcaaagc aagcgcacaa gtagataggt
     3661 attatcatgg ggatgagttg cttgaatttg tgaacggatt ggattatctc gtcaatattc
     3721 ttccgaacac gaaagaaacc aaaaagatta tagatgcaaa caccttaaat gctttacctt
     3781 cccatgccat attcataaac gtggggcgtg gctcaacagt agatgaacct gcgctcttga
     3841 aggttctaca agaaaataaa atcgctggtg cagttttgga tgttttcgaa caggagcctt
     3901 tgccaagcga gcatccattc tgggatgcgc cgaatctgag cataactttc catacatccg
     3961 caccgagttt accggaagat atagcccaag tttttataga aaactacaag ctttatagtg
     4021 atagaaagcc attgaagcac caagttgatt ttgaaagagg atactaatga ctgtcacaat
     4081 aaatgatatt cgacaagcag cgcaacgcat caagccatac attcaccata ccccagtaat
     4141 gaccaatgaa agtttaaacc aaagggttgg cgcacaagtt tttatgaaat gcgagaacct
     4201 gcaaaaagtt ggagcattca aatttcgcgg ggcatcaaat gccgtctggt cattgacaga
     4261 tatagaagcc tcaagtggag tggtcactca ctcatctggg aatcatgcac aggcgttggc
     4321 tttggctgca aagatacgtg gtattcctgc atatatcgtc atgccgagta atgcaccaca
     4381 ggtgaagaaa aatgctgtag caggatatgg cggacaaatt acattttgcg agccaacgct
     4441 cgcagcgaga gaaagcacta tggagagcat caggcaacgc acgggcgcga ccgtggttca
     4501 tccatatgac aatgaacacg tcattgcagg tcaagggaca gcagtattgg aactgctgga
     4561 agatatccca gatttggatg tgattatcgc acctgttggt ggcggtggat tgttaagcgg
     4621 aacatc
//
LOCUS       DAOQNJ010000155         4605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11565_length_4605_cov_11.500440, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000155 DAOQNJ010000000
VERSION     DAOQNJ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11565_length_4605_cov_11.500440"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PLG52_05875"
     CDS             <1..1137
                     /locus_tag="PLG52_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41006.1"
                     /translation="PGIDPVGACVGQKGVRIQAIVKELHDEKIDIILWDADPVAYISK
                     AISPAKVTGVYLTEEERTATVVVGEDQLSLAIGRDGQNARLAAKLTSWRIDIKSLSEA
                     AGDSLAKLQGDAELQNMLKAAVEVMPQVEEILAKKAENRLITPEEFSTLEQFVDRVER
                     RMILAKEEASREEDERIAAARAEIPAAAFNMPLDQATNLKEHVFNILTEAGYETVGQL
                     IFDMKTDQNKVLGLAGIGGKAITNIEETLAALTFPEPVAEPKVEETPAVVAEPVVTET
                     APVVETPVVEKPAEKKKEPKRVIEEEEEDGEVSKDGVNLDELFQMKPEMFQAGEGADE
                     ESDDKKKGKKVKKKGVALEFDENLGEVVSRKKHKRSDGGDDEAW"
     gene            1182..1481
                     /locus_tag="PLG52_05880"
     CDS             1182..1481
                     /locus_tag="PLG52_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNB41007.1"
                     /translation="MVKKPVQRVKHIPQRTCVGCREILSKRQLLRIVRTAEGVRVDPT
                     SKLAGRGAYLHDKRSCWARGLKGGLAHALKTEISAEDRARLEEFMNTLPEAETDG"
     gene            1566..3335
                     /gene="infB"
                     /locus_tag="PLG52_05885"
     CDS             1566..3335
                     /gene="infB"
                     /locus_tag="PLG52_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNB41008.1"
                     /translation="MSENGNKIELPASIVVRDLAQKIEKSPIDLIKKLMTNGVMATIN
                     QTVDYDTAAIVVAEYGFEAVPEVVEEESKEEAGEVPMWRQMIAGEDKAQLKQRPPVVT
                     ILGHVDHGKTSLLDAIRSTDVAAGEAGGITQHIGAYQVEMKGRLITFLDTPGHAAFTQ
                     MRARGAQGADIVILVVAADDGVMPQTKEAIAHAKAARVPIVVALNKVDKPNANPDRVK
                     QQLAELELVPDDWGGNTMVVPVSAKQKKGIDDLLEGVLLVADSNEIKANPSGKVIGTV
                     IEAELDKSKGVLATLLVQNGTLESGDVVVAGTAHGKLRAISDYKGKPVKKAGPSTPVA
                     VMGLSDVPSAGDLFEVVPSEKEARAIVAERMEVLKAQAQSRKKLSLEDLFSKVQAGEA
                     KELNLIVKADVQGSLDPIITELNKLGQGEIGLKVLHAETGNIGNNDVMLASASKAIMI
                     GFNVQADVDARRMAEKEGVDIRLYEIIYRMTEDIEKALNGMLEPDLKEKVLGKATVLQ
                     VFTASKFGRVAGCRVTDGELKRGAKVRLYRDSDIIYEGDLSSLRHEKDDVKEIRQGFE
                     CGVGFKNFSDIQSGDTLVCYILE"
     gene            3400..3750
                     /gene="rbfA"
                     /locus_tag="PLG52_05890"
     CDS             3400..3750
                     /gene="rbfA"
                     /locus_tag="PLG52_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNB41009.1"
                     /translation="MPSGIRIKRIEDRIQQELSEMLIHEISDPRLKQIYVTGIKVDRE
                     LAFAEVYVSAVEGASRSEEVLAGLESASGFLRRTLSARVELRSFPKLRFHWDPTPENA
                     DHIEKLLAGLRNKK"
     gene            3790..>4605
                     /locus_tag="PLG52_05895"
     CDS             3790..>4605
                     /locus_tag="PLG52_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNB41010.1"
                     /translation="MTDQISGDIKNRLADAKKVVIASHVRPDGDAIGSLLGLGLALIE
                     AGKTVEMILVDGAPASFKFLEGSELIKKEPTGGHDTFITVDCADFKRTGKIFESFGQP
                     DINIDHHKTNEKFGKLNLIEAEEVATAAILTNHLPTWGLKITKSVAAALLTGILTDTL
                     GFRTSNVTPEALRQAATLMESGVDLPEIYMQSLVRKSFPAAKYWGAGLSSLESKDGIV
                     WGTLTLEDRKSASYGGNDDADLINMISAIEGHKVGMVFVQQTDNKVKISWRALQ"
BASE COUNT         1281 a         1109 c         1214 g         1001 t
ORIGIN      
        1 cccggtatcg accctgtcgg cgcatgtgtg ggtcaaaagg gtgttcgtat tcaagccatc
       61 gtcaaagaat tgcacgatga gaagatcgac atcattttgt gggacgccga cccggttgcc
      121 tacatctcca aagccattag ccccgccaaa gtgactggcg tatatctcac cgaagaagaa
      181 cgcacagcta ccgttgtagt tggcgaagac cagttgagtc ttgccatcgg tcgtgatgga
      241 caaaatgccc gcctcgctgc caaactgacc agctggcgca tcgacatcaa gtctttgtct
      301 gaagctgcag gcgactcgct tgccaaactt caaggcgatg ccgaattgca gaatatgctc
      361 aaggctgcgg tggaagtgat gccgcaggtg gaagaaattc tcgctaagaa ggctgaaaat
      421 cgcctcatta cgccggaaga attcagcaca ctcgaacaat tcgtggaccg tgtagaacgc
      481 cgcatgatcc ttgccaagga agaggcttct cgcgaagaag acgaacgcat tgccgccgct
      541 cgcgccgaaa tccctgccgc tgcgttcaac atgccgctcg atcaggcaac aaacctcaag
      601 gaacacgtct tcaacattct tactgaagct ggctatgaaa ccgttggtca gttgatcttc
      661 gatatgaaga ccgaccagaa caaagtgttg gggctggctg gtattggtgg taaagccatt
      721 acgaacatcg aagaaacgct cgcggctctc accttccccg aaccggttgc tgaacccaag
      781 gtggaggaaa ccccggcagt tgttgcagaa cccgttgtga cagaaacggc tccagtggta
      841 gaaacacctg ttgtggagaa acctgccgaa aagaagaaag aaccgaaacg cgtcatcgaa
      901 gaagaggaag aagatggcga agtctcgaag gacggcgtca acctcgacga gctgttccaa
      961 atgaagccag aaatgttcca agctggcgaa ggtgcggatg aagaatccga cgataagaag
     1021 aagggcaaga aagttaagaa gaagggtgtt gccctcgagt tcgacgaaaa ccttggcgaa
     1081 gttgtctcac gcaagaagca caagcgcagt gatggcggcg acgacgaggc atggtagtta
     1141 atgattcccg cctccataat ggaggcgggg agtaaacgga cgtggtcaaa aaacctgtac
     1201 aacgcgtaaa acatattccg cagcgcacct gtgtcgggtg ccgggaaatc ctgtcgaaac
     1261 gacaattact gagaattgtc cgcacagcag aaggcgtgcg tgtggacccc accagtaagt
     1321 tggcaggaag gggcgcttac ttgcacgaca agcgctcttg ttgggcgcgc ggattaaaag
     1381 gcggtttagc ccacgctttg aaaacggaaa ttagcgcaga agaccgcgcc cgtttggaag
     1441 aatttatgaa cactctgcca gaagcagaga ctgacggtta agccatgtga acgaataagc
     1501 cactcgctca caggctgcct gatacattga acaaagatat gttttgagat tcaaggaggt
     1561 ggcttatgag tgaaaacgga aataagatcg aattacctgc cagcattgta gtacgagacc
     1621 tggctcaaaa gatcgaaaaa agcccgattg acctgatcaa aaaattgatg accaatggtg
     1681 tcatggcaac cattaatcag acggtggatt acgataccgc cgcgattgtg gttgctgagt
     1741 atggctttga agccgtaccc gaagtcgttg aagaagaatc aaaagaagaa gcgggcgaag
     1801 tgcccatgtg gcgtcagatg atcgccgggg aagataaggc tcaactgaaa cagcgccccc
     1861 ccgttgtgac cattctgggt cacgtcgatc acggcaaaac cagcttgttg gatgccattc
     1921 gctccacgga tgtggctgct ggtgaagctg gcggtattac tcaacacatc ggcgcgtatc
     1981 aagtagaaat gaagggacgt ctcatcacct tcctcgatac gcccggtcac gccgcgttca
     2041 cgcaaatgcg cgctcgtggt gctcaaggtg ctgatatcgt catcttggta gtggctgccg
     2101 acgatggcgt gatgccccaa accaaagaag ccattgcgca cgccaaagcg gcgcgtgtgc
     2161 caatcgtggt ggcattgaac aaggtcgata aacccaatgc caacccagac cgtgtaaaac
     2221 aacaacttgc cgaactcgaa ctcgtccctg atgattgggg tggcaatacc atggtcgtgc
     2281 ccgtctctgc caagcagaaa aaaggcatcg acgatttgct cgaaggtgtt ttgctcgtgg
     2341 cagatagcaa cgagatcaaa gccaacccca gcggcaaagt aatcggtacc gtcatcgaag
     2401 cggaattgga taaatccaag ggtgtgctgg cgaccttgct cgttcagaac ggtacgctcg
     2461 aatcaggcga cgtggtggta gctggaaccg cgcatggcaa attgcgcgcc atctccgatt
     2521 acaagggcaa gccggtcaag aaggcgggtc cttccacacc ggtcgctgtg atggggctca
     2581 gtgacgttcc ctccgctgga gatttgttcg aagtcgtccc gtctgaaaaa gaagcgcgtg
     2641 ccattgtggc tgaacgtatg gaagtcttaa aggctcaggc acaatctcgc aagaaactct
     2701 cactcgaaga cctgtttagc aaagtccagg caggtgaagc gaaagaactc aacctcatcg
     2761 tcaaagccga tgtgcaaggt tcgcttgacc ccatcattac tgagttaaat aagctcggtc
     2821 aaggtgaaat tgggttgaaa gtgttacacg ccgaaacagg taatatcggc aacaatgacg
     2881 taatgctcgc ttccgcctca aaagccatca tgattggttt caacgtccaa gcagacgtgg
     2941 atgcccgccg catggctgaa aaagaaggcg tggacattcg cctttatgag atcatctacc
     3001 gcatgaccga ggatattgaa aaagccctca atggtatgtt ggagcccgat ctcaaggaaa
     3061 aggttcttgg caaggcaacc gtcttgcagg tctttactgc ctcgaagttt ggacgtgtgg
     3121 caggctgccg tgtaaccgac ggcgaattga agcgtggtgc caaggttcgc ctttaccgcg
     3181 actcagatat catctacgaa ggtgacctct cctctcttcg ccacgaaaaa gacgatgtga
     3241 aagagatccg ccaagggttt gaatgtggtg ttggtttcaa gaacttctcc gacatccaat
     3301 ctggcgacac cctcgtttgc tacatcttgg aataagtact tttactactt acatatcacg
     3361 tatcaaagtg atatgtaagt agtaacattt ggaacgacta tgccatctgg aattagaatc
     3421 aaacgaattg aagatcgtat tcagcaggaa ctttcggaaa tgctgattca tgagatcagt
     3481 gatccgcgtc tcaagcaaat ttatgtgacc ggcatcaaag tggaccgtga acttgccttt
     3541 gcggaagtgt acgtctctgc cgtggaaggg gcttcccgct ctgaagaagt gctggcaggg
     3601 ctagaatccg cttctgggtt tctgcgccgc accctttctg cccgtgtaga actacgttca
     3661 ttcccaaaat tacgatttca ttgggatccc acacctgaaa atgctgacca tatcgaaaaa
     3721 ctgttggcag ggttaaggaa caaaaaataa tcatccttat tgaaaataag gaattgtgga
     3781 gaaattatag tgacagatca gatctcaggg gatataaaaa atcgactcgc agatgctaag
     3841 aaggtggtga ttgcatcgca tgtacgccca gacggagacg ccatcggctc cctgctcgga
     3901 cttggactgg ctcttattga agcaggcaaa accgtcgaaa tgatcctggt agatggagcg
     3961 cccgcttctt tcaaatttct tgaagggagt gaactcatta aaaaagaacc caccggcgga
     4021 cacgacactt ttattactgt agattgtgcc gatttcaaac gaacggggaa aatctttgaa
     4081 agctttggtc agcctgatat caatattgac caccacaaaa ccaacgaaaa atttgggaaa
     4141 ctaaatctaa ttgaagcaga agaagttgcc acagcagcta ttctcaccaa ccacctcccc
     4201 acttgggggc tcaagatcac caagtcagtt gccgctgctt tattgaccgg tattctgacc
     4261 gatacccttg gcttccgcac atcgaacgtc acgcctgaag cgttgcgtca agctgcgact
     4321 ttaatggaat cgggtgtgga ccttccagaa atttacatgc aatcgctagt gagaaaatcc
     4381 ttcccggctg ccaagtattg gggcgccggg ctttcaagcc ttgaaagcaa agatggcatt
     4441 gtttggggaa cgctgacatt ggaagaccgc aagtctgcaa gttatggcgg taacgatgac
     4501 gcagatttga tcaatatgat ctctgccatc gaaggacata aagtgggaat ggtctttgta
     4561 caacagaccg ataataaagt aaagatctcc tggcgtgctc ttcag
//
LOCUS       DAOQNJ010000156         4605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11566_length_4605_cov_11.442637, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000156 DAOQNJ010000000
VERSION     DAOQNJ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11566_length_4605_cov_11.442637"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2328
                     /locus_tag="PLG52_05900"
     CDS             <1..2328
                     /locus_tag="PLG52_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41011.1"
                     /translation="TTEYRKLAEDVANVFYQTPRAVTSALRAASDSLNKVLLDRNMSS
                     SNRGQYTLAWLALAAVRESQSIFSLCGPMHAYWFSANGTRHFFEPTISGKGLGSSQTI
                     NIHYAQSDLSPNDMLLFCGRLPNAWVTPLEDAQPSSFDAMRRRLTSITNEDLNAVLIQ
                     TTTGAGVINLLRGNMVTKTEAPVEVVPTETITEAPVQEQPQAIEEPNLEEEPAPQTST
                     SDLPATYEPASTPEGGVHLLQPSTYSLPPQREETAFPASIPNADPLANLPHRTGPREF
                     PASIPRAKPRTEAEAPAIEENPIVEKIEPKPEPVQEIKTEKESVPEIKIPEGPREPSP
                     QAKQAAKIVVNLMQGFRTGSASLGERFRNFLPRLLPAEEQSTTSVSANAFMGFMAVLI
                     PLIVVTLAVVVYLRYGRNEQYEAYYNQALQMKQQALALTNPVEQRIAWENVLANLDQA
                     ENHNVTSDTMNLRQEAESKRDQLLGISRMQFNPAFTAKLGIDVSRMAATENDLYLLNA
                     ANGEVLRAIPAGNGGFELDNTFNCTPGVGNTVGPLVDILALPITNIYSATVLGIDASG
                     NLLYCKPGATPQTGLLPPPDTNWGRITSFILDSGNLYVLDAPSRAVWVYLGKESAFVD
                     RPYFFFGQQTPTQDVIDFLVIGDEMYLLHADGRISNCTYSRVDTNASKCQDPVNKINP
                     FPGYADMDLFATAHFTQILFAAPPDPSLLLLDADNQVVMRFAPRTLELQNQFHPTSGR
                     SNPIPRSPVSAVAVSPDHVLYLAVNGQVYFAPNMP"
     gene            2347..3327
                     /locus_tag="PLG52_05905"
     CDS             2347..3327
                     /locus_tag="PLG52_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41012.1"
                     /translation="MPSVLETIANLFRPAPYPKDNTTEPAQIIRSKVLVIVYDPVMDA
                     AGTKLSKYMKWKDTTDLISGFMLDMLDVTEGKVRYEVVERIDVNEFPAKTDGFRYTAQ
                     TYLDVWNRTSAPHMPQEVNYSAILAQFNILNRVARSEIDEVWVFGFPYGGFYESTMAG
                     PGAFWCNAPPLANTALSKRRFVIMGFSYERGIGEMFESYGHRAESIMEKTFSKLTGEA
                     NLWKRFIRYDKVAPGKAACGNVHFAPNSQADYDWGNKTPVKSECYDWLLNFPNFKGDI
                     RTVDSYEWGGGEIRAHHKWWFNHFPRVAGRKNGIHNNWWQYIAAPQNVII"
     gene            3449..>4605
                     /locus_tag="PLG52_05910"
     CDS             3449..>4605
                     /locus_tag="PLG52_05910"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNB41013.1"
                     /translation="MYNADFLVPFVYILAAIFYALVGLYAWRKRPAVGVVSFAWVMLS
                     MSIWSLTYALEIFLPTTAAKLQVLNIEYLGILGVPVFLFFFALDFTGRSHLLTPRFRL
                     LLWGVPAFILLMVWTNPLHSLMWGKVETFRSGSLSLLSVDFRSAFWVNIIYTYGLVIA
                     ACIILIMEFLQRPGSLRLQISFVILSLLASLLGSSTFVLNISPIVGVDLSPLFFLPAA
                     IGLAWVTLRYRLAEILSLEHITVLQNMKDSVIVLNETKKVIYINQVTEKLFNLTEDKV
                     IGQPFNFVSGRLANLLDPYISPNEQHHNEIEIGDGEEKKTYEVSISPIVSATRNTAEI
                     IIALHDITLRKEKEQELSRHGAIMSAVSRAAEQFLKTEDWEQNISNVLENLG"
BASE COUNT         1221 a         1161 c         1001 g         1222 t
ORIGIN      
        1 acaactgaat atcgcaaact cgctgaagat gttgcgaatg tgttctatca aactccgcgt
       61 gccgtaacca gtgctttgcg cgccgcttct gattcgttga ataaagtgtt gctcgaccgt
      121 aatatgagct ctagcaatcg tggtcaatac acgttggcat ggttggctct cgcggctgtg
      181 cgagaaagtc agagcatctt ttcgttgtgt ggtcccatgc atgcgtattg gtttagcgca
      241 aatggcacgc gtcatttctt tgaacctaca atttctggca aaggtttggg ctccagccaa
      301 actatcaaca ttcattacgc acaaagtgac ctaagcccaa acgatatgtt gcttttttgc
      361 gggcgtctcc ccaacgcttg ggtgactccg cttgaggatg cgcaaccctc ttctttcgac
      421 gccatgcgcc gccgcctcac ttcgattacc aatgaagacc tgaatgcggt gcttattcaa
      481 accacaactg gtgcaggcgt catcaacctt ctgcgcggca acatggtgac aaaaacagag
      541 gcacctgttg aagtcgtccc cacagaaacc attacagaag cgcctgttca agagcaacct
      601 caagcaatag aagaaccaaa tttggaggaa gaacccgcgc ctcaaacttc gacgtcagac
      661 ttgccagcca cctatgaacc tgcgtcaacg cctgaaggtg gcgttcatct cttacaacct
      721 tccacctatt ctcttccgcc tcaacgtgaa gagacggcat tccccgcatc tattccaaat
      781 gcagacccat tagcgaatct tccacaccga acaggtccac gcgaatttcc ggcatccatt
      841 ccgagagcca aacctcgaac agaagcagaa gctccggcaa tcgaagagaa tcctatcgtc
      901 gaaaaaatcg agccgaagcc agaacctgtt caagaaatca aaaccgaaaa agaatccgtt
      961 cctgaaataa aaattcctga aggtccgcgt gaaccttcgc cacaagccaa acaagcagcg
     1021 aagatcgtcg tcaacctcat gcagggattt cgcaccggca gcgcttcact cggtgaacgc
     1081 ttccgcaatt ttttgccgcg ccttttaccc gcggaagaac aaagcaccac ttcggtttca
     1141 gccaacgcct ttatgggatt catggcagtg ctcattccgc tcattgttgt aacgcttgct
     1201 gtggttgttt acctgcgtta tggacgcaac gaacagtatg aagcctatta caaccaagcg
     1261 ttacaaatga agcaacaagc tctggctctc actaacccag ttgaacaacg cattgcctgg
     1321 gaaaacgtgc ttgctaattt ggatcaggct gagaatcaca acgtcaccag cgacacgatg
     1381 aatcttcgtc aggaagctga atccaaacgc gaccagttgc taggcatttc tcgcatgcaa
     1441 ttcaacccgg cgttcactgc aaaacttgga attgatgtca gccgcatggc agccacagaa
     1501 aatgacctct atctgctcaa tgctgcaaat ggtgaagtgc tgcgtgccat ccctgctggg
     1561 aatggtggct ttgagttaga caatacgttc aactgtactc ccggtgttgg caatacagta
     1621 ggtccattag tggatattct tgctttgccc atcaccaata tttacagcgc aaccgtcttg
     1681 ggtattgatg cgagcggcaa tcttttatat tgcaaaccgg gcgccactcc gcagaccggt
     1741 ctcttacccc ctcccgatac caactggggg cgtatcacca gcttcatctt ggactctgga
     1801 aatctgtatg tgctcgatgc gccttcgcgt gcagtatggg tgtatcttgg aaaagaatcg
     1861 gcatttgttg accgtcccta cttcttcttc ggtcaacaaa cccctacaca agatgtgatc
     1921 gatttcctcg tcattggtga tgagatgtat ctcctgcatg ccgatggtcg catttcaaat
     1981 tgtacatata gccgcgttga cacgaacgct tcgaaatgtc aggacccggt taataagatc
     2041 aaccccttcc ctgggtatgc tgatatggac ttgttcgcaa cagcacactt tactcagatc
     2101 ttgtttgcag cgccaccaga tccatccttg ttattattag atgcagataa ccaagttgtg
     2161 atgcgttttg caccgcgcac gcttgaactt caaaatcaat tccaccctac atctggaaga
     2221 tcgaacccta ttccgcgcag ccctgtcagc gccgtagcgg ttagccctga tcatgtgttg
     2281 tatcttgcag tcaacggtca ggtttatttc gcccccaaca tgccataacg ttgtaaggag
     2341 gtttccatgc ctagcgttct cgaaacaata gccaatctct ttcgtcccgc tccctacccg
     2401 aaagataaca cgaccgaacc tgcgcaaatc atccgcagca aagtattggt tatcgtttat
     2461 gacccggtca tggatgcggc aggcaccaaa ctttccaagt atatgaagtg gaaagacacc
     2521 acagacctga tttcaggctt catgctggat atgttggatg tcaccgaggg caaagtccgt
     2581 tatgaggttg ttgaacgcat agacgttaac gaatttcccg cgaaaacaga cggcttccgg
     2641 tatacggctc aaacctacct cgatgtctgg aaccgcacct ctgcgcctca tatgccgcag
     2701 gaagtgaatt actccgcgat tctcgcccaa ttcaatatct taaaccgagt cgcgcgcagt
     2761 gaaatagacg aagtgtgggt attcggtttc ccatatggtg ggttctatga atctaccatg
     2821 gcaggtcccg gcgctttttg gtgcaatgcg ccaccattag caaatacagc gttgagcaaa
     2881 cgtcgcttcg taatcatggg attctcgtac gaacgtggca ttggcgaaat gttcgagtca
     2941 tatggtcatc gtgccgaatc gatcatggaa aagaccttca gcaaacttac cggcgaagca
     3001 aatctatgga agcgcttcat ccgctacgac aaggtagcgc cgggcaaagc cgcctgcgga
     3061 aacgtccact ttgcacccaa tagccaggca gattatgact ggggcaacaa gactcccgtc
     3121 aaaagtgaat gttacgactg gttattaaac ttcccaaatt tcaaagggga tatccgcact
     3181 gtggactctt atgaatgggg cggcggtgaa atccgcgctc accacaaatg gtggtttaac
     3241 cactttccac gagtggctgg tagaaagaat ggcattcaca ataactggtg gcaatacatt
     3301 gctgctccac aaaatgtgat catataacaa aatggcactt cgttctggaa cgaagtgcca
     3361 ttttgtatag ataaaaaccc tacccaaact atcatcttat tattatgaac tttagtcagt
     3421 gctaaaatta caatagattt gtaagggaat gtataacgct gattttctag tcccgtttgt
     3481 ttacatcctt gctgccatct tctatgcgct cgtagggcta tatgcatgga ggaaacgccc
     3541 ggcagtaggt gttgtctcat ttgcatgggt catgctctcc atgtcaatct ggtcgcttac
     3601 ctatgcgctg gaaatcttcc ttcctacaac cgctgccaaa ttacaagtat taaatattga
     3661 atatctcgga atccttgggg ttccagtttt cttatttttt ttcgcattag atttcacagg
     3721 cagaagccac ctacttacgc cgcgttttag actgcttcta tggggcgttc ccgcttttat
     3781 acttcttatg gtgtggacaa atccactcca ctctttaatg tggggcaagg tcgaaacctt
     3841 tcgttctggc tccttaagtt tattaagcgt ggactttaga agtgcattct gggtaaatat
     3901 tatttacaca tatgggcttg ttattgcagc ctgcatcatt ctcattatgg aatttttgca
     3961 gcgtccaggc agtttaaggc ttcagatcag ttttgtaatc ttaagcctcc ttgcttcgct
     4021 tttgggctct tcgacgttcg ttctaaatat cagtcctatt gttggcgtag atctttcacc
     4081 gctattcttt ctgcctgctg ccattgggct tgcctgggta actttacgat atcgtctcgc
     4141 tgagatcctc tcacttgaac acatcactgt tcttcaaaac atgaaggaca gtgttattgt
     4201 tctaaatgag acaaaaaaag ttatctatat caaccaagtc acagaaaagt tattcaatct
     4261 cactgaagac aaagtcattg ggcagccctt caatttcgta tcgggaagat tagctaattt
     4321 attagatccc tatatcagtc ccaacgaaca gcatcataat gagattgaaa ttggagatgg
     4381 tgaagagaaa aaaacctatg aagtttctat ttctccaatc gtttcagcga cacgaaatac
     4441 agctgaaatc ataatcgcat tacacgacat aactctccgt aaagaaaaag agcaagaact
     4501 tagtcgtcac ggggcaatta tgtctgcggt cagtagagca gctgaacaat ttctcaaaac
     4561 tgaagattgg gaacaaaata tatcaaacgt gctagaaaat ttagg
//
LOCUS       DAOQNJ010000157         4566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11721_length_4566_cov_5.506761, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000157 DAOQNJ010000000
VERSION     DAOQNJ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11721_length_4566_cov_5.506761"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLG52_05915"
     CDS             complement(<1..847)
                     /locus_tag="PLG52_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB41014.1"
                     /translation="MPVDYEDALDYSSNWMEWIQKNEFPTPEQAHKIIEESTTNFAEY
                     YNRNWLEYRKSVTESGDWAAVEWGGSGAVFKDVLGREYLDFLGGYGMMDLGWSHPEVV
                     NTVKAQLERSPMPSQELLDPLRGVLGRLLASITPGDLKYSWFGASGTEANEAAMKIAK
                     LYTGKTAFIVGVKAFHGKTMGSLSLMGKSDYRAPMGAMYSGQVYHVPFGDADAVEKQL
                     EICEKVGIGVAAVMFEPIQGESGAIVPPDDFWPRIRAATKKHGVLLIADEVQTGLGRT
                     GKLWGV"
     gene            complement(872..1297)
                     /locus_tag="PLG52_05920"
     CDS             complement(872..1297)
                     /locus_tag="PLG52_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB41015.1"
                     /translation="MIKKILLPTDGSEHAEKTIHFAIDMAKSLGASLDVMYAYQPAPF
                     LRKRAAAMMEEYRNILEEDAKEVVTEVADRLQTEGLTVNAVVVEGPAAEAILRAADEI
                     KPDLIIMGSRGEGGFADMLLGGVAEHVIHYSKVPVLVVK"
     gene            complement(1309..2730)
                     /locus_tag="PLG52_05925"
     CDS             complement(1309..2730)
                     /locus_tag="PLG52_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNB41016.1"
                     /translation="MTVEVNPPASGSEIVVKVIKRKITLLPLVMILFFTVSGGAYGLE
                     DLVGSSGPGMAILLIIVTPLIWSLPTALMVAEMSTALPVQGGYYAWAKVALGPFWAFS
                     EGWLSWMTSWVDMAIYPVLFADYLSALLVQQFNIHIIEENALVHWGVTLVVIWVFAYL
                     NIRGAKSIGDSSNLFGLFILAPFLVMSVIGIVKLILHPVAIWEPFVPPDSNILGAFGI
                     GMFVVMWNYLGWDNTSTVSAEVDNPRRNFPRALAITIPLVTLAYLLPVVAGLGSSPDW
                     AGWTAGYFPEVAANIGGKWLGAWLAIGGLVSAVGLFSAIMLSISRLPFVMAEDGYLPK
                     AITKIHPTFETPWVSIVVCAAIYSIFTLSAFSSLVVVDVILYSVALTLEFASLIALRI
                     KAPNMKRPFKVPGGWLGIFLICVFPAGVLALAVVSTIQEEGISALYMSIGAIALTPVM
                     YVILRKFVKKDQPDVEVPVELEG"
     gene            complement(2740..3861)
                     /locus_tag="PLG52_05930"
     CDS             complement(2740..3861)
                     /locus_tag="PLG52_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB41017.1"
                     /translation="MHKNRFASMFLVFAIVSGMLAACAPSAPVVTSKELNLYAFSEYV
                     PADLLSSFEKETGIKVNYEAYSSNEEMLAGLDANPDKYDLLIPSDYTVEGLIQKKALR
                     PLDLKLIPNYSNIDPAFLSPYFDPGGDTGGRRPYATDNDKYSLPYQWGTTGIAYDTTK
                     VDQPITKWEDLWRPELAGHIVVLDDARELMGAALLSLGYDKNTTDPKRLEEARDKLKK
                     LAPGILAYDSDAPEKYLISGEAWVGVVFNGNAALAMRQNQNIQYVFPEEGATIWFDNF
                     VIPVNALHADAAEAFINFSLAPENSALITRDFPYSSPNTAALDYLAKNDASLYEQYRS
                     NNASNPPPDALANARLAKNLSADASDLYTQFWSEVKNTK"
     gene            complement(3890..>4566)
                     /locus_tag="PLG52_05935"
     CDS             complement(3890..>4566)
                     /locus_tag="PLG52_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB41018.1"
                     /translation="TKIVADSAFGCAGQRCLAVSLAVTVGESKNEFTELICDAASSRT
                     VGYGMDSGVQMGPVINTASQQRVEQLITLGVKEGAGVPVDGRGTKVKGYEGGSFVRPT
                     ILSDVQPGSEIARTEIFGPVLSLMHVKTIDDAIQLVNSGQYGNQASLFTTSGNAARRF
                     RYEADAGNIGINIGVAAPMAFFPFSGWKDSFFGDMHGQSTDAVEFFTQKKVVVERWPK
                     EWTRKF"
BASE COUNT         1212 a         1272 c         1021 g         1061 t
ORIGIN      
        1 caacgcccca cagtttcccg gtccggccca gacccgtttg cacttcatcc gcaatgagca
       61 atacgccatg cttcttcgtc gcagcacgga tgcggggcca gaaatcatcg ggcggaacga
      121 tggcacccga ctcgccttga atcggctcaa acatcaccgc cgcgacgcca atgccaacct
      181 tctcgcagat ttccaattgt ttttcaacgg catctgcatc gccgaacgga acgtgataca
      241 cctgtccgct atacatggcg cccatcggag cacgatagtc tgacttaccc atcaatgaaa
      301 gcgaacccat ggtcttgccg tggaaggctt tcacacctac gataaatgcc gttttgcctg
      361 tatacagctt tgcgatcttc atcgccgctt cgttggcttc ggtgccgcta gcaccaaacc
      421 aactgtattt tagatcgccc ggcgtgatcg atgccaacaa acgccccaag acaccccgca
      481 agggatcaag caattcctgc gaaggcatgg gcgaacgctc caactgcgcc ttgaccgtgt
      541 tcaccacctc aggatgactc catcccaaat ccatcatgcc atagccgcca aggaaatcaa
      601 gatattcacg cccaagtaca tctttgaata ccgcgcccga accgccccat tctactgccg
      661 cccaatcgcc agactcagtc accgatttac ggtactcaag ccaattgcgg ttgtaatact
      721 cggcaaaatt ggttgtagac tcttcaataa tcttgtgagc ctgctcaggg gtcgggaatt
      781 catttttctg aatccactcc atccagttag atgaataatc caaagcgtct tcgtaatcta
      841 cgggcataca caaatctcca ttttagaaat attatttaac gaccaacacg ggtacttttg
      901 agtaatgaat cacatgctca gcaaccccgc ccaaaagcat atctgcaaag ccgccttcgc
      961 ctcgcgaacc catgatgatc aggtcaggct tgatctcatc tgctgcacgc aaaatcgctt
     1021 ctgcggcagg accttcaact accacggcgt tgacggtcag tccttctgtt tgtaaacgat
     1081 cagcaacttc tgttacaact tccttcgcat cttcttcaag aatattcctg tattcttcca
     1141 tcattgcggc ggcgcgtttc cgcaaaaatg gagctggctg atacgcatac atcacatcca
     1201 aacttgcacc caatgacttt gccatatcaa tggcaaagtg aattgtcttt tcggcatgtt
     1261 ccgaaccatc cgtaggaagt aatatttttt taatcatgtc ggtaccctct atccttccag
     1321 ctcaaccggg acttcaacat ccggctggtc tttctttaca aatttcctca atatcacata
     1381 catcaccggc gtcagggcga tcgcgccaat agacatatac agcgcgctga taccttcctc
     1441 ttgaatcgtg ctgaccactg caagagccaa cacgcctgcc gggaaaacac aaatcaagaa
     1501 tattcccaac caaccacccg gcactttgaa tgggcgtttc atattgggag ccttgatgcg
     1561 tagtgcgatc aaagaggcga attccaatgt caacgctact gaatacaaaa tcacatcaac
     1621 aaccacgagg ctggagaatg cgctcaaggt aaagatcgaa taaattgcag cacaaaccac
     1681 aatggagacc caaggagttt caaacgtggg gtgaattttt gtaattgcct tgggcaaata
     1741 accatcctct gccatgacaa agggcagacg cgaaatggaa agcatgatgg cactgaacaa
     1801 acccacggcg ctgaccaaac cgccaattgc cagccaggct ccaagccatt tcccgccgat
     1861 atttgccgcc acttccggga agtaacccgc cgtccaacca gcccaatcag gcgaagagcc
     1921 taaacctgca accactggga gcaaatatgc aagcgtaacc agcggaatgg taatcgccaa
     1981 tgcacgcgga aaattacgcc gtgggttatc cacttctgca ctaactgtgg atgtgttatc
     2041 ccagccaagg tagttccaca tcacaacaaa cataccaatg ccaaatgcgc ctaaaatatt
     2101 tgaatccggc gggacgaacg gttcccagat cgccactgga tgaaggatca gcttcacaat
     2161 tccgatcact gacatgacaa gaaacggagc cagaataaac aagccaaaca ggttcgatga
     2221 gtcaccaatg ctttttgcgc cgcgaatatt gaggtaggca aaaacccaga tcacaaccaa
     2281 agtcacaccc cagtgaacaa gggcattttc ctcaatgata tgaatattga attgctgcac
     2341 tagcaaagca gaaagataat cagcaaacaa tacggggtaa atcgccatat ccacccagga
     2401 tgtcatccag ctaagccagc cttctgaaaa tgcccagaac ggaccaagtg ccacttttgc
     2461 ccaagcataa tacccgccct gtaccggcaa ggcggtggac atttccgcca ccatgagcgc
     2521 ggttggcaga ctccaaatga gcggcgtcac gatgatcagc aagatcgcca tgcccgggcc
     2581 agacgagccg acaagatctt caagtccgta ggcaccaccg ctaacggtaa aaaacaggat
     2641 catcaccagc ggcaagagtg tgatcttgcg cttgatgacc ttaactacaa tttccgaacc
     2701 actggctgga ggatttactt cgacagtcat gtcaggctcc tatttcgtgt tttttacttc
     2761 agaccagaat tgtgtgtaaa gatcggatgc atcagcactg aggttctttg ctagacgggc
     2821 atttgccagc gcatcgggcg gcgggttgga tgcattgttt gaacgatatt gttcgtaaag
     2881 tgacgcatca ttcttcgcta aataatcaag tgcagccgta ttggggctgg aataaggaaa
     2941 atcacgtgta atcaaggcgc tgttttccgg cgcaagtgaa aaattgataa acgcctccgc
     3001 ggcatccgca tggagggcat ttactggaat gacaaagttg tcgaaccaaa tggttgcgcc
     3061 ttcttcgggg aaaacatatt gaatattctg attctggcgc attgccagcg cggcatttcc
     3121 attgaacacc acacccaccc aggcttcgcc agaaatcaaa tatttttcag gcgcatctga
     3181 atcataagca agaatacccg gagcaagctt cttaagctta tctctcgcct cttccagccg
     3241 tttggggtcg gttgtattct tatcataacc aagtgaaagc agtgcagcgc ccatcaattc
     3301 gcgcgcatca tccaagacca caatgtgccc ggcaagttcg gggcgccata aatcctccca
     3361 ctttgtgatc ggctggtcga ccttggttgt gtcataggca atgcccgttg tgccccattg
     3421 atagggcagg ctgtatttat cattatcagt ggcatacggg cgtcgtccac ccgtatcgcc
     3481 accggggtca aaatagggag aaagaaatgc agggtcgata ttgctatagt ttgggatcaa
     3541 cttcaagtcg agaggacgaa gcgctttttt ctggataagt ccttcaaccg tgtaatcgct
     3601 cggaatcaac aagtcatatt tatccgggtt cgcatccagc ccggcaagca tctcttcatt
     3661 ggatgaatat gcttcgtaat taacctttat gcctgtttcc ttttcaaaag aagagagcag
     3721 gtctgcggga acatattctg agaacgcata cagattcaat tctttggaag tcacaaccgg
     3781 agccgagggt gcgcacgcgg caagcattcc acttacgatt gcgaacacta aaaacataga
     3841 tgcgaagcga ttcttatgca tatttctcct ctcccatatt ttcgggaaac taaaacttcc
     3901 tcgtccattc tttgggccag cgttcgacca caactttttt ctgcgtaaag aactcaactg
     3961 catcggtact ttgtccgtgc atgtcgccaa agaatgaatc tttccaaccg ctgaagggga
     4021 agaaagccat cggcgctgcg acgccaatat tgatgccaat attgcccgca tccgcttcgt
     4081 agcggaagcg tcgggctgca tttccgctgg tggtgaacaa actggcttga ttgccatact
     4141 gcccgctatt gaccaactgg atggcatcat cgatcgtctt gacatgcatg agactaagca
     4201 ctggtccgaa aatttcagtg cgggcaattt cgcttcccgg ctggacgtct gacagaatcg
     4261 tcggtctgac aaaggatcca ccctcgtagc ctttcacctt cgtcccgcgc ccatcgactg
     4321 ggactcccgc gccttccttt acaccaaggg tgatcaactg ctccacacgc tgttgtgacg
     4381 ctgtgttgat aaccggtccc atctgcacgc cagagtccat gccgtagccg acggtacgag
     4441 aagacgccgc atcacagatc aactcggtga attcgttctt tgattcgccc acggtcacag
     4501 cgagagacac tgccaggcag cgctgacccg cacacccgaa ggctgagtcg gcgacgatct
     4561 tcgtcg
//
LOCUS       DAOQNJ010000158         4559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11753_length_4559_cov_8.516430, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000158 DAOQNJ010000000
VERSION     DAOQNJ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11753_length_4559_cov_8.516430"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLG52_05940"
     CDS             <1..385
                     /locus_tag="PLG52_05940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB41019.1"
                     /translation="RANYEDIRQPCERLRTNLSQSGVSEEIAGLCELALQELLTNLVD
                     HAYEGNLNGQITVRMASKNGQIFLQTEDTGLPARIKMEDVHMPNPEDLAEGGYGMAII
                     RSLMDDIRYESMQGKNIWTLIKNLK"
     gene            410..754
                     /locus_tag="PLG52_05945"
     CDS             410..754
                     /locus_tag="PLG52_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB41020.1"
                     /translation="MSLEINSTEQVIKIAAITLKGRLDAFEAPALRKTCDAYIADEFS
                     HFVFDLTEVAMLDSAGLAVLVSVLKRARLKNGDVRLVWPREEAASRILKLTKFDQVFV
                     SIEKGEIVPRGF"
     gene            756..1130
                     /locus_tag="PLG52_05950"
     CDS             756..1130
                     /locus_tag="PLG52_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB41021.1"
                     /translation="MSKAKILVVDDEPNNQRILTYTLNKAGYESAAIADGENTLVWLA
                     SNTPDLAILDVSMPGMDGITLLTHIRSMPQYSRLPVIILTGSGDDDERLRAEGLGIQA
                     FLTKPASSKTVLEAVANLLPHE"
     gene            1123..2403
                     /locus_tag="PLG52_05955"
     CDS             1123..2403
                     /locus_tag="PLG52_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNB41022.1"
                     /translation="MNKLKELLRSKLASASALLMLSMTVVNAGNYIYNLIMGRWLGPS
                     LFADLSLIVTLLLVVTFLTAPIQMTAARYSAIHTADGDDKTLASLRRFIWLVALGVGL
                     TLTAFFAIFSTTLKVFFHTQSALPFVIFGAALPFGMMQAVERGMLQGRTSFKVLAISY
                     QVEMWSRLLVSILLVALGLSVNGAVIGISLSFVFTWLVARSAVVGLNSQENISKEMRT
                     ELLAFAAPVLVSQLGQILINNSDVILVKRFFETSEAGKYAALALIGRIVFFATWSVVT
                     TMFPIVAQKQKMGEPHAHLLWVSLGIVAGVSLPIVLLTWLVPDWVVLVLFGKKYLNIS
                     GLLWMYALATSLYAMANVVINYRLSLGMGRETGFAIAAGLAQVFGIIYFHNSLAQVVY
                     VQVVVMGVLFLALMWHNFLHEKARTLLQAVTHHA"
     gene            2396..>4559
                     /locus_tag="PLG52_05960"
     CDS             2396..>4559
                     /locus_tag="PLG52_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB41023.1"
                     /translation="MLDLLMSVCGTALLAFDETGKIIRANESVAEMFGCSVEELLKKK
                     INSLLPKATARRHSKLFQQFLEEDTPHRKMGKYRSVIAQRKNGEEFSVEISIGKAEMD
                     GKWIAIASLRDLAMEKHNEEFFRSTAIASGENPNPVFRISPDGKIIFSNTSAEEMLKS
                     IGHHDQNIPAAWVNSLQQALDSGTQVVSIIQHGERAYSCVFAPAPTKEYVDMYALNVT
                     DREEEKARLALSDEILNSIGNLVMVANSNAEIVYISPSVINLLGYRPDEILGEGWWEI
                     ERISGGDVQVEKEYIRKAAAGKIDVDGKPYEHHVRHKDGSWRWLMLSDTKGPRDLVIG
                     IGYDITPIKAAEEELERQRDFARTLTNQMGQGLTVTDENGCFEFVNPSYAQMLDYEPS
                     ELIGKTPYDFTLPEDHWNLLNAQVKRSLGSVTTYETRLLGRNNKIVYALITGVPRSVN
                     GNYKGAITVVTDLTERLTMEKQLRQYADEIQQANIQLADARDRALEASYLKSAFLATM
                     SHEIRTPMNAILGMSELLLDTELNEEQREFANVIEASTQNLLAILNDILDLSKIEAGK
                     LAIHPAPFNPASVIMETMKLFSPKAQQKNINVSVNVPPDAQTLVLGDAGRIRQVLSNL
                     LSNAIKFTERGGAVFVTLSSSQIKDVMMTTFTVQDTGLGIPDAVKPKLFNPFTQADDS
                     QTRRYGGSGLGLAISKRLIDLMHGEIGFETLEGKGSTFW"
BASE COUNT         1193 a          969 c         1108 g         1289 t
ORIGIN      
        1 tcgtgccaat tatgaagata ttcgccagcc atgtgagcgc ttgcgtacaa atttaagtca
       61 gagcggggtt tcagaagaga ttgccggctt gtgcgaactt gccttgcaag aactacttac
      121 gaacctggtt gaccatgcct acgaagggaa tttgaacggg cagatcacag tgcggatggc
      181 aagcaagaat ggacagattt tcctgcaaac ggaagatacc ggcttgcctg ctcgtataaa
      241 aatggaagat gttcatatgc caaaccccga agaccttgcc gaaggcggtt atggcatggc
      301 gatcatccgt tcgttgatgg atgatattcg ctatgagtct atgcaaggca agaatatctg
      361 gacgcttatc aagaatttga aataatggaa tcaagagaaa gaggtaacta tgtcactcga
      421 aattaattct acagaacaag tcataaaaat tgctgccatc acactaaaag gcaggctcga
      481 tgcctttgaa gcccccgcgc tacgaaaaac ttgtgacgca tacattgccg atgaattcag
      541 ccattttgtt tttgacctga ccgaggtagc catgctggac tcggctgggt tggcagtcct
      601 tgtcagtgtt ctcaaacggg cccgcctcaa gaatggcgat gtgcgtcttg tctggccccg
      661 cgaagaagcg gcatcccgca tactcaaact caccaagttc gatcaggttt ttgtctctat
      721 cgaaaaaggc gagatcgtac caagaggttt ttaacatgag caaagctaaa attctcgtag
      781 tggatgatga acctaacaat cagcgcatcc ttacttacac ccttaataaa gccggctatg
      841 aaagtgcagc cattgctgat ggcgaaaaca cgttggtttg gcttgcatct aatactcccg
      901 atcttgccat tctagatgta tccatgccag gcatggatgg aattactttg ctaacacaca
      961 tccgctctat gccgcagtat tctcgtttgc ccgtcattat cctcacaggt agtggcgatg
     1021 atgatgaacg tctgcgtgcc gaagggctgg gcattcaagc ttttcttacc aaaccagcca
     1081 gttctaaaac ggtcttggaa gcagtagcaa atctactccc tcatgaataa gctcaaagaa
     1141 ctacttcgct ccaaactggc atctgccagc gcgctgctta tgcttagcat gaccgtcgtc
     1201 aatgcgggca attacatcta taaccttatt atgggcaggt ggctgggtcc gtcactgttt
     1261 gcagaccttt cactcattgt tactttgctg ctagttgtga cctttctaac cgcccctatt
     1321 cagatgaccg ctgcgcgtta ttctgccatt cataccgctg atggagatga taaaaccctt
     1381 gcctctcttc gacgttttat ctggctcgtc gccttggggg tgggtctcac gctgacagct
     1441 ttctttgcca ttttttcaac tacattaaag gttttctttc atacgcaatc tgctcttcct
     1501 tttgttattt ttggcgcagc acttcctttt ggcatgatgc aagctgttga acgtggaatg
     1561 cttcaggggc gcaccagttt caaggttttg gcaatcagtt atcaagttga gatgtggagc
     1621 cgtttgttgg tcagtatcct tctggttgcc ttggggcttt cggtcaatgg tgctgtgatc
     1681 ggcattagtt tgtctttcgt attcacatgg ctggtggcgc gctctgcagt agtcgggctg
     1741 aattcacaag aaaatatttc caaagaaatg cgtaccgaat tgctggcgtt tgccgcaccc
     1801 gttctggttt ctcagttagg gcaaattctt atcaacaata gcgatgtgat cttggtgaag
     1861 cgcttttttg agacgagtga agctgggaaa tatgcagcgc tggcgctgat tgggcgtatt
     1921 gtgttttttg ctacttggtc agttgtaacg accatgttcc ctattgtggc gcaaaaacaa
     1981 aagatgggcg agccacatgc tcacttgtta tgggtgtcac tgggaattgt ggcaggtgtt
     2041 tctttgccaa ttgtgttgtt gacatggctt gtgccagatt gggtggtttt ggttttgttc
     2101 ggtaaaaagt atttgaatat ctctggcttg ttgtggatgt atgcattggc aacttcattg
     2161 tacgccatgg caaatgtggt gattaattat cgattgtcat tgggaatggg gcgtgagact
     2221 ggctttgcca tagcggcagg gcttgcccaa gtattcggta ttatttattt tcataattcc
     2281 ttggcacaag ttgtgtatgt gcaagtagtg gtaatgggtg tgctgtttct tgcgctcatg
     2341 tggcataact ttttgcatga aaaagcacgt accctattgc aggcggtgac acatcatgct
     2401 tgatctcttg atgtctgttt gcgggactgc cctgttggca tttgacgaaa ctggcaagat
     2461 catccgtgcc aatgagtctg ttgctgagat gtttggctgt tcggtagagg aactgctaaa
     2521 gaaaaagata aattcgctcc tgcccaaagc caccgcacgt agacattcca aactctttca
     2581 acaatttctc gaagaggata cccctcatcg aaagatgggg aaatatcgtt ctgtaatcgc
     2641 tcaacgtaaa aatggtgaag aattctcagt ggagatttcc attggcaaag ctgagatgga
     2701 tggtaaatgg atcgctattg cttcattgcg agaccttgcc atggaaaaac ataatgaaga
     2761 atttttccgc tccacggcaa ttgcttcggg tgagaaccca aaccctgttt tccgtatcag
     2821 ccctgatggg aagattatct tttccaatac cagcgctgaa gagatgttaa agagcattgg
     2881 gcatcacgat cagaatatcc ctgctgcttg ggtcaattcc ctgcaacaag cactcgatag
     2941 tggaacacaa gtggtgtcta ttattcagca tggggaaagg gcatattctt gcgtttttgc
     3001 tccagcgccc acaaaagaat atgtggatat gtatgctcta aatgttacag atcgggaaga
     3061 agagaaagca cgcctcgctt tatctgacga gatattgaat tcaatcggca atttggttat
     3121 ggttgcaaat agcaacgctg agatcgtcta tatcagccct tctgtgataa accttcttgg
     3181 gtatcgtcct gatgagattt taggagaggg ctggtgggag attgaacgca tctctggggg
     3241 agatgtgcaa gtagagaagg aatatatccg taaggcagca gccggtaaga ttgatgtaga
     3301 cggcaaaccg tatgagcatc atgtacgcca taaagacggt tcatggcgtt ggttgatgtt
     3361 gtctgacacg aaaggtcccc gagacctcgt gatcggaatt gggtatgaca ttacccccat
     3421 caaagcagcg gaagaggaac tcgaacgtca acgtgatttt gctcgaaccc ttaccaatca
     3481 aatgggacag ggattaactg ttacagacga aaatggctgt ttcgaatttg ttaatccttc
     3541 ttatgcacag atgttggatt atgagccatc agaattgatc ggtaaaacac cctatgactt
     3601 tacccttcca gaggatcact ggaatttgtt aaatgctcaa gtaaaacgtt ctttgggcag
     3661 tgtaacaaca tatgagaccc gtttgctcgg acgtaacaat aagattgttt atgctctgat
     3721 caccggtgtg ccacgcagcg tgaatggaaa ttataaaggt gcaatcaccg tagtaaccga
     3781 tttaactgaa cggttaacca tggaaaaaca attacgccaa tatgctgatg aaattcagca
     3841 ggcaaatatt caattagcag atgcgcgtga ccgggcactt gaagcttctt atttgaaaag
     3901 cgcttttctc gcaaccatga gtcacgaaat tcgaacaccg atgaatgcta ttttggggat
     3961 gagtgaactg ttattagata ctgaactaaa tgaagagcaa agagaatttg cgaatgtcat
     4021 tgaagcttct acccagaatt tattggcgat cttaaatgac attttagatc tttccaagat
     4081 cgaagctgga aagttggcga tccatcctgc accgtttaat cctgcctcag taatcatgga
     4141 aaccatgaag ctctttagcc ctaaagcaca gcaaaagaat atcaatgttt ccgttaatgt
     4201 tccgcctgat gctcaaactc tagtcttggg agatgcaggg cgaatccgcc aggtattgag
     4261 caacctcttg agtaacgcta tcaaattcac agaacgtgga ggcgctgttt ttgtcacact
     4321 ctcaagctca cagatcaaag atgtgatgat gaccactttt acagtccaag atacggggtt
     4381 gggcatccct gatgcagtca agccaaaatt atttaatcca tttactcaag cagatgattc
     4441 tcagacgcgg cgctatggag ggtctggctt ggggctggca atttcaaaac gcttgatcga
     4501 tctcatgcat ggagaaatcg gttttgagac attggaaggg aagggttcca ccttctggt
//
LOCUS       DAOQNJ010000159         4540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11819_length_4540_cov_6.901449, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000159 DAOQNJ010000000
VERSION     DAOQNJ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11819_length_4540_cov_6.901449"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1655)
                     /locus_tag="PLG52_05965"
     CDS             complement(<1..>1655)
                     /locus_tag="PLG52_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNB41024.1"
                     /translation="SVSQNHQPDSLMREWARIGYQRVNTVLEPGQFSGRGGILDVWAP
                     TELNPVRLDFFGDEIETIRSFDPASQRTLEKLENILITPSREFLADTTDSEAQLSEFH
                     IPLLHQQPASLLDYLPQKTLVLVDDGSLIEVMANEVEEQAVKFRQESIKEGTLAESFP
                     VPYVPWSELNDNLSEFVHLELGHSTEAEIEIPLASHFGHDERFGGRLKPFIDYLAPIV
                     DDGEQVFIVSRQAQRLRELWFEHYPDSEHTNLEFTEASLSEGFTLKDQSLLSIHLITD
                     SEVFGWERPQPRTRQRQTADTPEAMYADLEAGDYVVHVDHGIGRFAGLIQRQLDGHER
                     EYLTVEYERGDILYVPVHQADRLTRYVGADGGKPSLDNLGGQAWAETKEKVKEAVQKV
                     AEDLLDLYARRQVVEGYAFSPDTQWQKELEDSFPYVETEDQKRAIVDIKRDMERARPM
                     DRLLCGDVGYGKTEVALRAAFKAVMSGKQVAVLVPTTVLAQQHFETFSQRLAAFPVKV
                     EMLSRFRTPREQTEILHQLSIGEVDIIIGTHRLISSDVVFKDLG"
     assembly_gap    1656..1755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1756..2257)
                     /locus_tag="PLG52_05970"
     CDS             complement(<1756..2257)
                     /locus_tag="PLG52_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41025.1"
                     /translation="MQQLLSQLRTLPSYQQVLSELTARKPVSGLGLPRAARLPLLAAL
                     HADINQPILLITDRADHALSMFDELGFWVKSPRYLFAEPNPLFYEEAAWGVTTRRERL
                     QTLTVLSTFHLPFTKKPETPPIIVASVRSLMTRTLPRRDFLKACKKLSVSQNHQPDSL
                     MREWARI"
     gene            complement(2272..2850)
                     /gene="pth"
                     /locus_tag="PLG52_05975"
     CDS             complement(2272..2850)
                     /gene="pth"
                     /locus_tag="PLG52_05975"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNB41026.1"
                     /translation="MTETFLIIGLGNPGREYKDTRHNIGFMMIDHLAETIGARGMKVQ
                     SKAIVISGLHQERKLILAKPQTYMNLSGQSVQGLLHFYKIPLENLIIAHDDMDIPFGT
                     IRIRPTGGPGGQRGMANTIELLGTKDFPRLRLGIGRPPGRMDPKDYVLQDFSKDELKL
                     MPELLTRASEAALEFVTKGLNAAMNKYNGSVE"
     gene            complement(2865..3494)
                     /locus_tag="PLG52_05980"
     CDS             complement(2865..3494)
                     /locus_tag="PLG52_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41027.1"
                     /translation="MLNLPIAMLKLFLILFLIFQGNTPSITSPQTGETLRGQVQIQGL
                     MDIPNFASAELAFTFDASASDPAASWFPIQTFPQPIQNPTLATWDTTAVTDGEYALRL
                     RIFLLDGTSQDVIVNTLKIRNNEPLPTPTITPTEAVFEFQPLDQTPGVSNEQNTPTPV
                     VLYSTATPLPANPASLTTSSIVTIFWQSAVLVVILFAFFSLLLRLRKNT"
     gene            3644..4018
                     /locus_tag="PLG52_05985"
     CDS             3644..4018
                     /locus_tag="PLG52_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNB41028.1"
                     /translation="MAPSRAEQLVERLRNMQAAAPDIEASAIVSVDGLIMASALQQGV
                     EEDRVSAMSAAMLSLGERISGELGRGGLEQVYIKGDKGSIVLTAIGDEAVLTAMARQE
                     AKLGMVFLEMRRAAEDLVKLVG"
     gene            4164..>4540
                     /locus_tag="PLG52_05990"
     CDS             4164..>4540
                     /locus_tag="PLG52_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNB41029.1"
                     /translation="MATKYLFFTGGVVSSVGKGVTAAATGLLLKERGFKVAVQKLDPY
                     INVDPGTMSPYQHGEVYVLDDGAETDLDLGHYERFIDIRLSRVSNFTTGQVYAETIAK
                     ERRGDYLGGTIQVIPHITNEIKRR"
BASE COUNT         1155 a         1050 c         1176 g         1059 t
ORIGIN      
        1 cccaagtctt tgaaaacaac gtcagatgaa atcagacgat gtgtgccgat aataatgtcc
       61 acttcgccaa tcgaaagctg atgcaaaatc tccgtctgtt cgcgaggcgt gcggaagcgt
      121 gaaagcattt ccactttgac cggaaatgcg gcgagacgct gagagaacgt ttcaaagtgc
      181 tgttgcgcca ataccgtcgt cggcacgagc actgccactt gcttgccgct catcacggct
      241 ttgaaagcag cccgcaaagc gacttccgtt ttgccatagc ccacatcccc acacaacaga
      301 cggtccattg ggcgggcgcg ttccatatcg cgtttgatat caacaatagc gcgtttttgg
      361 tcttcggttt caacataagg aaagctgtct tcgagctctt tttgccactg agtatcggga
      421 gaaaaagcat agccctctac tacttgtcta cgcgcatata aatccagtaa gtcttcggct
      481 actttctgaa ccgcttcctt aaccttttcc ttggtctctg cccatgcctg cccgcctaag
      541 ttatctaacg aaggcttgcc accatcggcg ccaacatagc gcgtaagtcg atccgcttga
      601 tggacgggaa catagagaat atcgccgcgc tcatattcaa cggtgaggta ttctcgctca
      661 tgtccgtcca actgtcgctg gataagaccc gcaaaacgac caatgccatg atccacatga
      721 acaacgtaat cccccgcttc cagatccgcg tacatcgctt cgggtgtatc cgctgtttga
      781 cgttggcggg tgcgcggctg tggacgttcc cagccaaaga cttctgagtc agtaatgaga
      841 tgaatagaga gtagagattg atctttgagc gtgaaacctt cagaaagtga ggcttcagta
      901 aactcgaggt ttgtatgttc tgagtcaggg taatgctcaa accacaattc gcgcaaccgt
      961 tgcgcctgac gcgaaacgat aaagacctgc tcaccatcat ccacaatcgg agcaaggtaa
     1021 tcaataaatg gtttgagccg cccaccaaag cgctcatcat gaccgaaatg agaagcgagc
     1081 ggaatttcaa tttcagcctc tgtggaatgc cccaactcaa gatgaacgaa ttcactcaag
     1141 ttatcgttca actcagacca tggcacatac ggcacaggga aactctcggc gagcgttccc
     1201 tctttgatgc tttcctgcct aaattttacc gcctgctctt ccacttcatt tgccatcact
     1261 tcgatcaggc tgccatcatc caccagaacc aaggtctttt gcggcaaata atccaacaac
     1321 gatgctggtt gttggtgaag caaaggaata tgaaattcag agagctgcgc ttccgagtcg
     1381 gtggtatctg ctaaaaattc gcgcgaaggc gtaataagaa tattttcaag tttctcaagc
     1441 gtgcgctgtg aagctgggtc aaaactgcga atggtttcga tctcatcgcc aaagaaatca
     1501 agccgcaccg gattcagttc cgtgggcgcc cacacatcga ggatgccacc acgcccagag
     1561 aattgacccg gctccaagac cgtgttgaca cgctggtagc cgatccgcgc ccactcgcgc
     1621 atcagactat cgggttggtg gttctggctt actgannnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnncgatc cgcgcccact cgcgcatcag actatcgggt tggtggttct
     1801 ggcttactga cagcttttta cacgctttga gaaagtctcg ccgcggcaaa gtacgcgtca
     1861 tcaacgaacg aaccgatgca acaataatgg gcggagtttc aggtttcttt gtaaatggca
     1921 agtggaatgt agaaagaacc gtaagcgttt gtaaccgttc acgccgggtg gtcactcccc
     1981 aagccgcctc ttcatagaaa agcgggttgg gctccgcaaa aagataacgc ggcgacttca
     2041 cccaaaagcc aagctcatcg aacatagaca aggcatggtc ggcacgatcg gtgatcagca
     2101 agatgggctg attaatatcc gcgtgaagag cagctaataa tggcaaacgt gccgcacgtg
     2161 gcaagcccaa tccagagaca ggtttgcgtg cggttagctc gctcagcact tgctgatacg
     2221 aagggagagt gcgaagttga gaaagaagct gttgcatgga ctgtaaaaaa tttattctac
     2281 cgagccgttg tatttgttca tcgccgcgtt caagcctttg gttacaaatt caagagccgc
     2341 ctctgacgca cgagtgagaa gctcgggcat gagtttcagc tcgtctttag agaaatcttg
     2401 caaaacgtaa tccttggggt ccatacgccc gggcgggcga ccaatgccaa gccgcaagcg
     2461 cggaaagtct ttagtgccaa gtaattcaat ggtatttgcc atgccgcgct gaccaccggg
     2521 tccgccggtg ggacgaatgc gaatggtgcc gaatggaatg tccatatcgt cgtgcgcaat
     2581 gatcaggttc tcaagcggaa ttttatagaa atgcaaaagt ccctgcaccg attgtcctga
     2641 aagattcatg taggtctgag gcttggcaag gatcaatttg cgttcctgat gcaaaccaga
     2701 aatgacgatg gctttggatt gaaccttcat tccgcgcgcg ccaattgtct cggcaagatg
     2761 gtcgatcatc ataaaaccga tattgtggcg ggtgtcttta tattcacgcc ccgggtttcc
     2821 gagaccaata atgagaaaag tttctgtcat gagaaaaatc caagctaggt gttttttcga
     2881 agtcgaagaa ggagggagaa aaaagcaaac aggatcacaa ccaatacggc gctttgccaa
     2941 aaaatagtaa cgatggatga agttgtcaac gaggctgggt tagcgggcaa aggagttgca
     3001 gtggaatata aaacgacagg tgttggggta ttttgttcgt tggatactcc aggagtttgg
     3061 tcgagcggtt gaaattcaaa gacggcttct gttggggtaa tggtcggtgt gggaagcggt
     3121 tcattattgc gaattttcag ggtgttgaca atgacatctt gagaggtacc gtctaggagg
     3181 aagatgcgta atcgcaaagc atattcccca tcggtgacgg cggttgtatc ccaagtggcg
     3241 agggtgggat tctgaatcgg ctggggaaag gtttggatgg gaaaccagct tgcggcgggg
     3301 tctgaggcgg aagcgtcaaa cgtaaaagcc aactctgccg aggcaaagtt gggaatatcc
     3361 atcagccctt gaatttggac ttgtccacgt agggtttcgc ccgtttgtgg ggatgtgatt
     3421 gaaggtgtat ttccctgaaa aatgaggaaa agaatcaaaa acagcttgag catggcaatc
     3481 ggtaagttta acatgaagag aaggcaaggt caaattttgc ctatttttct gtaattgtga
     3541 gattgaaaat gaattgaaat gtgggggcaa ttctcgaatg aagatttttc tggtaaactc
     3601 tagccgttca tagaacgtaa acgtttatta ggagatctaa gttatggcac catcccgcgc
     3661 tgaacaactc gtggaacgac ttcgcaacat gcaagccgcc gccccagaca ttgaagcttc
     3721 tgccattgtt tcggtggatg gcttaatcat ggcatctgcc ctgcaacagg gtgtggaaga
     3781 agatcgtgta tccgcgatgt cggcggcaat gttgagcctt ggcgaacgca tctctggtga
     3841 gttgggtcgc ggtggtttgg aacaggtcta tatcaaaggt gataaaggtt ccatcgtatt
     3901 gaccgctatc ggcgacgaag ccgtgctcac tgctatggca cgtcaagaag cgaagctcgg
     3961 tatggtcttc ctcgaaatga ggcgagccgc tgaagatctg gtgaagttag tcggatgaga
     4021 cgtaaaagtc actccacttc aaaataaggt aaccccgaat ggggagggca taacgccctc
     4081 cccattctct ttgtatatac cccctcattc gcctttcctg tctgtgcaac agggtgggct
     4141 gtaaaaccaa ggagaagaaa aggatggcaa caaagtattt attttttacg ggcggtgtgg
     4201 tttcgtcggt gggcaaaggc gtgacagcag cagcaactgg tttgctgctt aaggaacgcg
     4261 gcttcaaagt ggcggttcaa aaactggatc cgtacatcaa cgtggaccca ggtacgatgt
     4321 cgccgtacca acatggtgag gtgtatgtgc tcgatgacgg cgcagaaacg gaccttgatc
     4381 tgggtcacta cgaacgattc attgacatcc gtttgagccg agtcagcaac ttcacgaccg
     4441 ggcaggtcta tgctgagacc attgccaagg agcgtcgtgg tgactatctc ggcgggacga
     4501 ttcaggtcat tccgcacatc acgaacgaga tcaaacgtcg
//
LOCUS       DAOQNJ010000160         4539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11824_length_4539_cov_8.386708, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000160 DAOQNJ010000000
VERSION     DAOQNJ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11824_length_4539_cov_8.386708"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="PLG52_05995"
     CDS             <1..1559
                     /locus_tag="PLG52_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41030.1"
                     /translation="LCFPMLILVTCLLLFITALVLVILRATQPNARYAWLVAAGGATL
                     AFVSVFVWLAQIPFQLTLPVWKPESLFSTPILFSADGVSWALAISIAALTLAVIFTVV
                     TRSVVFNSLSWAGILVLGGMGILAVTANNPLTLLLMWALLDMTELGTQLSSVSGEKNN
                     ERVVISFATRMIGIGLLLWAYIESFTGGGMVVFQTMPPDTGVYLVIAAGLRLGVLPLH
                     LPYAADSTLRRGFGTALRLIGAASTLSILGHIQILPTNLTPILMILTSVAAIYGGWTW
                     LRAPDELNGRPYWMIGMASLAILSALSGNATGAIAWGSALVLVGGALFLFSIQDIRLS
                     RALLIGGWALSSLPLSLTASTWASSFNISTIFSLAAQVLLTAGYIRHALRRNGTETLD
                     DQPGWAKVVYPAGILLLLVTQILLGFIGWDGAFKFGNLIFSLLASILALGLVGGSRRF
                     RIFNPVRAHWVTTAGSGVNGLYQWLWSLYRGLAQVAQAITNALEGEGGIMWTLLFLIL
                     FISIIVQGIQ"
     gene            1560..2183
                     /locus_tag="PLG52_06000"
     CDS             1560..2183
                     /locus_tag="PLG52_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41031.1"
                     /translation="MVSDIVSWISVALTLVTATTILVNRDWRVSLGALALQYLAAFWL
                     VTRHLPFAMGSVKLITGWMVVAILGMTRLSLTNLDQNEQDAFYPRGAAFRITLMGIVT
                     LVALGSTPRIEEAIPGLGMPVIAGGLLLIGAGVAHLGVTNDLLRVALGLLTVLTGFEI
                     IYAAVESAILVTGLLAVVNLGLGVMGSYLLMAGSIPLISPEEEEELL"
     gene            2180..3577
                     /locus_tag="PLG52_06005"
     CDS             2180..3577
                     /locus_tag="PLG52_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNB41032.1"
                     /translation="MSAPILWIAVPFVLGVLILFVLSERYAAYVGGTISTILAAIAFF
                     IPIDTALLLGNVSLKISPAIEFFGRSFELSTADGRLLTLIYGLAALWFFGTEASGTAH
                     RFVSLGLMITALLTASIAVEPFLYAALLLEMAAMLVVPLLVPLYQRPGRGVTRFIIYQ
                     TLAMPFILFSGWMLAGVEASPGDLGTIVQSGVMVTMGFAFLFAVFPLYNWIPQLIEET
                     NPYAVGFLLWALPTFTTIFALGFLDRYTWLRNTPQISNAILFSGVIMTATGGIFGALQ
                     RHLGRIFGYAAIAETGLLLIALGLRSSEVVNIAFLILIPRGLELSVWALALSIIKRKA
                     YPLKFSDVQGLARKYPVATSALIIAHLSVAGFPLLAGFPSRLALWQELSLQSLSASLW
                     VFLGLLGLLVASVRTLATLVMASDDAPWELNETWVQTFMLGIGVLGIFLLGLFPQILQ
                     PFLSSLPALFEHLIQ"
     gene            3621..>4539
                     /locus_tag="PLG52_06010"
     CDS             3621..>4539
                     /locus_tag="PLG52_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41033.1"
                     /translation="MEFEQIVKRLEFLDKQQRETKETLTSLKESLASFETTVNAVSKQ
                     IKTLSKQVTDITPAAKRVDQFETMVGKQRNELVKMIEDVDKAHTRSERDTTKQFQSDI
                     NELSKTLTQLKVSTDLTEIKKQLKERGNEIQRALANIADFKTVVEDTKRSNEDVKLSI
                     KAADEARKNDLKRIADLQGELTALRKRIDENREKSTIQGDTIRNIETRITELLSSELE
                     RKQAQAVFLDQQRVAQVDRERAWKDWQDKFVSFQKEAETLDQQIQRLDETLRNAKKAQ
                     DTYMELNSKLERRINEVTEMQRLAEDRLRQ"
BASE COUNT         1022 a         1099 c         1097 g         1321 t
ORIGIN      
        1 aattatgttt tcccatgttg atcttggtta cctgtctttt gctttttatt acagcacttg
       61 tgctggtgat tttacgcgct acccaaccca atgcacgcta tgcctggttg gtggcagccg
      121 gtggtgccac acttgcgttc gtgagtgtat ttgtatggct tgctcaaatt ccctttcaac
      181 taacattgcc ggtctggaaa cctgagagtt tattttctac acccattctt tttagtgctg
      241 atggggtctc ttgggcgtta gcgatcagca tcgcggcgtt gactcttgcg gtgatcttca
      301 cggtcgtgac acgctcagtc gtcttcaact cgctttcatg ggctgggatt cttgtgctag
      361 gtggcatggg tattttggca gtaaccgcca ataacccact gacgttactc ttaatgtggg
      421 ctctcctcga catgacagag ttaggcacac aattaagctc agtgagcggc gagaaaaata
      481 atgaacgtgt cgtgatctcg ttcgcaacac gcatgatcgg tatcggtcta ttattgtggg
      541 catatattga gagttttacg ggcggcggaa tggttgtttt tcaaacaatg ccacctgata
      601 cgggtgttta tctggtcatt gcggcggggt taaggcttgg tgtattgcca ttacacctgc
      661 cctacgccgc tgattcgact ttacgccgcg ggtttggaac cgccttacgt ttgatcggcg
      721 cagcatccac ccttagcatt ctcgggcata ttcaaatcct tccaaccaac ttgaccccga
      781 tattgatgat ccttacttcg gtggctgcca tttatggtgg ttggacttgg ctgcgcgcgc
      841 ccgatgaatt aaacggtcgt ccatattgga tgatcggcat ggcttcgctt gcgattctct
      901 cagcgttaag cgggaatgcc acaggtgcaa ttgcatgggg cagtgcgctt gtactcgtag
      961 gcggcgcgct ctttcttttt tccattcaag atatacgtct aagtcgtgcc ttactcattg
     1021 gtggatgggc gctttcctct ctgcctctct cgctcacagc cagcacatgg gcgagcagtt
     1081 ttaacatctc gactattttt tcattagcgg cacaggtatt acttaccgct gggtatattc
     1141 ggcatgcctt gcgcaggaat ggcaccgaga ctttggacga tcaacccggt tgggcaaagg
     1201 tcgtttaccc agctggcatt ctcctgcttc ttgtgactca aattctgctt ggatttatcg
     1261 gttgggatgg tgctttcaaa ttcggaaatt tgattttctc cttgctcgcc tccatccttg
     1321 ctttgggctt ggtcggtgga agcagacggt ttcgcatctt caacccggtc cgtgcgcatt
     1381 gggtcactac tgctggctca ggggtcaatg gactttatca atggctctgg tcgctctatc
     1441 gtgggcttgc ccaagtagca caagccatta ccaatgccct tgaaggcgaa ggcggcatca
     1501 tgtggacgtt gctcttctta attctattta tctcaatcat cgtacagggc attcaataaa
     1561 tggtctctga tattgtttct tggatatcgg tcgcattaac ccttgtcaca gccacaacga
     1621 ttctcgtgaa tcgtgattgg cgtgtcagct tgggtgcgct tgctcttcaa tatctcgccg
     1681 cattctggtt agtgactcgt cacttgcctt tcgcgatggg gtcggtcaaa ttgattaccg
     1741 gctggatggt tgttgctatt ctcggcatga cgcgtctcag cttgaccaat cttgatcaaa
     1801 atgaacaaga tgctttttat ccgcgcggtg cagcgtttcg catcacgttg atgggaattg
     1861 tcacgctcgt agcattagga tccacaccac gcattgaaga agccattccg gggcttggca
     1921 tgcctgtcat cgcaggtgga ttactactga tcggtgcagg ggtggcgcac cttggagtca
     1981 ctaacgatct attacgtgtg gcgctcggtc tactgactgt gctcactggg tttgagatta
     2041 tttatgcagc cgtggaaagt gccattcttg ttacgggtct tttggcagtt gtgaatttag
     2101 ggttgggcgt gatgggctcc tatttattga tggcaggttc tattccactt atttcccccg
     2161 aagaagagga agaactgcta tgagtgcccc cattctatgg attgccgttc cttttgtgct
     2221 cggtgtatta atcctattcg tattaagcga acgctatgct gcatatgtcg gtgggacgat
     2281 atcaactatt ctggcagcaa ttgccttttt cattccaatt gatactgccc ttctcttggg
     2341 aaatgtctcc cttaagattt ctccggctat tgagtttttt ggtcgcagtt ttgagctctc
     2401 cacagcagat ggacggttat taacgctgat ctatggcttg gctgctctct ggtttttcgg
     2461 caccgaagct tctggtacgg cacatcgttt tgtctctttg ggattaatga tcactgcatt
     2521 gttgactgct tctattgcag tagagccttt cttgtatgcc gcactcttgt tggagatggc
     2581 agccatgtta gtagtaccac tgctcgttcc gctgtaccag cgaccgggtc gtggtgtgac
     2641 ccgcttcatt atttaccaga cgcttgccat gccgttcatc ctcttttctg gctggatgct
     2701 tgcaggcgtt gaagcgagtc caggagactt ggggacgatt gttcaatctg gtgtgatggt
     2761 gacgatgggc ttcgcctttc tctttgccgt ctttccactc tacaattgga ttccacaact
     2821 gatagaagaa accaatccgt atgcagttgg ttttctactt tgggcgttgc ctacatttac
     2881 aaccatcttt gctttaggct ttttagaccg ctacacgtgg cttcgcaata caccgcaaat
     2941 ttcaaacgct attcttttct caggtgtcat catgaccgct actggcggca tcttcggcgc
     3001 gttgcaacgt catcttgggc gcatctttgg gtatgccgct attgcagaga cagggctatt
     3061 attgattgca ctcggcttac gctcttctga ggttgtcaat atagccttcc tcatcctcat
     3121 ccctcgcgga cttgagttaa gcgtttgggc attggcacta tccatcatca aacgaaaagc
     3181 ctatccgctt aagttcagtg acgttcaagg attagcgcga aagtatccag tagcaacgag
     3241 cgcattgata attgcacatc tttctgtggc ggggtttccg cttttggcag gctttccctc
     3301 gcggttggcg ttatggcaag agctatcgct tcaatcgcta agtgcttcat tatgggtctt
     3361 tcttggctta ttaggattgt tggttgcctc ggtccgcaca ttggcgacac tcgttatggc
     3421 aagcgacgat gcgccgtggg aactaaatga aacctgggtt cagaccttta tgctgggtat
     3481 cggtgttctt ggcattttcc tgctgggact ctttccccag atcctgcaac ctttcctttc
     3541 tagtttgcct gccttattcg aacacctcat tcaataatcc cgttttgcaa aagggtataa
     3601 tttaacgaaa cggagtcctc atggaatttg aacagatcgt aaaaagactt gaatttttag
     3661 ataaacaaca acgcgagact aaggaaaccc ttacctcgtt aaaagaaagc cttgcctcct
     3721 ttgaaactac agtaaatgcc gtttcaaagc agattaaaac cctgagcaag caggtcacag
     3781 acattacccc tgccgccaag cgtgtagatc aatttgagac catggttggt aaacagcgta
     3841 atgagctggt caaaatgatc gaagatgtgg acaaagccca cactcgttca gagcgcgata
     3901 ccacaaaaca gtttcaatct gacataaacg agctcagcaa aacattgact cagctaaagg
     3961 tcagcaccga tctcacagaa atcaagaaac agctcaaaga gcgcggtaat gaaattcaac
     4021 gcgccttggc aaatattgca gacttcaaaa cagttgtaga agatacaaag cgctcgaacg
     4081 aagatgtgaa gttatccatc aaagcggcag atgaagcccg caaaaatgac ctcaagcgca
     4141 tcgcagacct gcaaggtgag ctcactgcat tacgcaaacg tatagatgaa aaccgggaaa
     4201 agtccacgat tcaaggcgat accattcgca acattgaaac gcgcattacc gaattacttt
     4261 cttcagaact ggaacgcaag caagcccaag ctgtgttctt agatcaacaa cgcgtagccc
     4321 aagtggaccg tgaacgtgcc tggaaagatt ggcaggacaa attcgtttcc ttccaaaaag
     4381 aagccgaaac tttggatcaa caaattcaac gtttagatga aaccttgcgc aacgccaaaa
     4441 aagcgcagga tacgtacatg gaactcaact ccaagttaga acgccgcatc aacgaagtga
     4501 ccgaaatgca gcgtctcgcc gaagaccgcc tgcgccagg
//
LOCUS       DAOQNJ010000161         4532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11858_length_4532_cov_8.676792, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000161 DAOQNJ010000000
VERSION     DAOQNJ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11858_length_4532_cov_8.676792"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLG52_06015"
     CDS             complement(<1..232)
                     /locus_tag="PLG52_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41034.1"
                     /translation="MDTIAPILQVKMSIYKFSVQQKNNDPRNESYRRGAHALGFHQLQ
                     RVECQDLYFIESQLSQEGLQQLALKLLSDPVTQ"
     gene            324..977
                     /locus_tag="PLG52_06020"
     CDS             324..977
                     /locus_tag="PLG52_06020"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNB41035.1"
                     /translation="MAKKKYSINWEDDLPVSFEVNGVQYQNIEDVPDEKDRLKLEAML
                     NSSFDAEINDPQLEKMMKETQQMDGASAEAWIVRIFTNVAVVMLLVAGVSTVFVLQKL
                     GREESAPGVVVDVVEQRYEDAETDNVSYYYYPVVEFKARDGKRRTVQLTEGSNPQSNE
                     VGDEVTILYEPENPNDARIKSFGSSALMWILPSITGILGAGFLGAVLVFQKYLANVS"
     gene            complement(1009..1587)
                     /locus_tag="PLG52_06025"
     CDS             complement(1009..1587)
                     /locus_tag="PLG52_06025"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HNB41036.1"
                     /translation="MSIFEIVYWAALVVEMVIRAPLNKKRKEEKISEQRVSQQEKLIL
                     FLLLIGMMILPLIYSATSWLDFANYNLPPWAGYLGALIMLSALFVFWRSHADLGLNWS
                     PSLEIRDKHELITHGIYGVIRHPMYASQWLWVLAQPLLLQNWLVGFINLLVFIPFYLL
                     RVQAEEKMMIDRFGEQYKTYMTKTGRVVPKLG"
     gene            complement(1584..1838)
                     /locus_tag="PLG52_06030"
     CDS             complement(1584..1838)
                     /locus_tag="PLG52_06030"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HNB41037.1"
                     /translation="MSERTYTEEFRVEGEKIIAKIKELLHEGNIRKVIIKDKDGKVLM
                     EIPVTIGVVGVLIAPQLAALGAIAALLTEATVIVEKTETL"
     gene            complement(1851..2441)
                     /locus_tag="PLG52_06035"
     CDS             complement(1851..2441)
                     /locus_tag="PLG52_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNB41038.1"
                     /translation="MPREYIGQNPTAFQRRPHLTKDDAWIRENINTFKVGHFATSVDG
                     QPFINTSTFWYDEAEHQIVFHSNLAGRIRSNIEHNPKVSFEASVLGQLLPSNVALEFS
                     LQYRSIVAFGTARLVTDPVEMRRLLYGLIGKYFSELTVGQEYREINDKELRATSVYAI
                     KIEEWSGKENWEARADQSDEWQPLDEKWFEYYRNKS"
     gene            complement(2451..3029)
                     /locus_tag="PLG52_06040"
     CDS             complement(2451..3029)
                     /locus_tag="PLG52_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41039.1"
                     /translation="MPRKPALNTDEILNSEYQYIAQNVFQSNEDRSRVTSFYFVSVGS
                     FVAAILGTRFEPDQSTISLAFFFLFTILTFMGALTIAQLARLRAAWHEAVEAMNQIKD
                     YYIKHMPELEPAFKWRKRQIPPTNKPYSIANLMAVEVALLSALTTGAGIYFLMTYIGI
                     SNWVTWSAVIVSVIFGYIAQWTWYRYLLVDNQ"
     gene            complement(3131..4366)
                     /locus_tag="PLG52_06045"
     CDS             complement(3131..4366)
                     /locus_tag="PLG52_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNB41040.1"
                     /translation="MNETFQTSLEKYADIIVNIGLNLHKEQRLNIAAGLPDAELVRLV
                     AKKAYQLGSPFVDVRWIDERLTRIRFENSKPEHLVEVPQWQIDREHEYGSRGDALLSF
                     ASQDPNLLEGISPDLIGRYRKAISEKYEPVAKYRNENTQTWCVVSTATPAWAQKVFPD
                     LPVEQAQEKLWEAIFDACRVNTADPVQAWREHAQKLHRQCDYLNEKRYAGLHYKAPGT
                     DLTLGLPDNHLWIGASGKTQNGIEYIPNLPTEEVFSMPHREKTEGYVTSSRPLILSGT
                     MIDEFTLTFENGRVNKVTAKQGEENIHKLLETDENASRFGEAALVPDSSPISQRKHLF
                     YNTLFDENASSHLALGTAYRYNVKGGTSMTKEEFIAAGGNDSLIHVDFMIGSDQMDID
                     GIRADGTREAVMRKGEWAF"
BASE COUNT         1141 a          973 c         1131 g         1287 t
ORIGIN      
        1 tttgtgtcac agggtcgctt aaaagtttga gagccaactg ttgcaatcct tcctgcgaca
       61 attggctctc gatgaagtag aggtcctgac attccaccct ttgaagctgg tggaatccaa
      121 gcgcgtgtgc accgcgccgg taactttcat tccgtgggtc gttgtttttt tgttgaacag
      181 aaaatttata gatgctcatt tttacctgta gaataggcgc tattgtatcc atgtaaattt
      241 gtctagtcaa tgcatgaatt tgttagcatg tcagacattt cgatgtgatt tcacctgata
      301 gaattttgcg ctcggaggta tgaatggcaa agaaaaaata ttccatcaat tgggaagatg
      361 atcttcctgt ttcgtttgaa gtgaatggtg tgcaatatca aaacattgaa gatgtgcccg
      421 atgaaaagga ccgcctcaag ttggaagcga tgttgaattc ttcttttgac gcagagatca
      481 atgaccctca acttgaaaag atgatgaagg aaactcagca gatggatggt gcctctgctg
      541 aagcgtggat cgtgcgtatc tttacgaatg ttgccgttgt gatgttatta gtggctgggg
      601 tttctactgt atttgtcttg caaaaactgg ggcgtgaaga gagtgcgccc ggtgttgttg
      661 tggatgtggt tgagcagcgt tatgaagatg ctgagacgga caacgtcagc tactactatt
      721 atccggtagt ggagttcaag gcacgagatg gaaagcgccg cacagttcaa ttgacggagg
      781 gtagtaaccc gcaatcgaat gaggtggggg atgaggtgac gattttgtat gagccagaaa
      841 acccgaatga cgcccgcatt aagtcttttg gtagctctgc cttgatgtgg attttgccaa
      901 gcatcacggg tattttaggc gctgggttct tgggtgcggt acttgtgttt cagaaatatt
      961 tagcaaacgt ttcgtgaaaa aatcccgcca tctggcggga ttttttttct aaccaagttt
     1021 tggcactact ctgcctgttt tggtcatata tgttttgtat tgttcgccga agcggtctat
     1081 catcattttt tcttctgctt gaacacgcaa gagatagaag ggaatgaaca ccagaaggtt
     1141 gataaaccca accagccaat tttgcagcag taaaggttgt gcaagcaccc ataaccattg
     1201 cgaagcatac atgggatgac ggatcacgcc ataaatgcca tgggtaatga gctcatgctt
     1261 atcgcgaatt tccaaggatg gagaccagtt caagccgagg tctgcgtgag agcgccagaa
     1321 aacgaataaa gcgcttagca taatcaatgc acccaaataa ccagcccaag gcggcaagtt
     1381 atagtttgca aaatccagcc atgaagtagc ggagtaaatg agtgggagaa tcatcatacc
     1441 aatgagaagc aagaagagga taagcttttc ctgttgcgaa acgcgctgtt cgcttatttt
     1501 ttcttctttg cgtttcttat taagcggggc acgaatgacc atctccacga ccaaagccgc
     1561 ccaataaacg atttcgaaaa tactcatagg gtctctgttt tttcaacgat cacagttgct
     1621 tctgtgagca aggcagcaat ggcgcctaat gcggcaagct gtggggcgat caacacaccg
     1681 acaacaccga tcgtgacggg gatttccatt aagaccttgc catctttatc tttgatgata
     1741 actttgcgaa tatttccctc gtgtagcaat tccttgatct tggcgatgat tttttcgcct
     1801 tccacacgaa actcttcggt ataggttctt tcactcatgc gtttatcctt tcaggatttg
     1861 ttcctgtaat actcgaacca tttttcatcg agcggctgcc attcatcgct ttgatcggca
     1921 cgtgcttccc aattttcttt gccgctccat tcttcgattt tgatggcata caccgacgtg
     1981 gctcgcagtt ctttgtcgtt gatctcgcga tattcctgtc caacggtcag ttcagaaaaa
     2041 tatttgccga tcaacccata taacagtcta cgcatttcta ccggatcggt tacaagccgg
     2101 gcggttccaa aagcgacgat gctccgatat tggagcgaaa actctaatgc aacatttgaa
     2161 ggcagtaatt gaccaagaac actcgcctca aaactgactt tgggattgtg ctcaatattc
     2221 gagcggattc tccccgccag attagagtgg aatacgatct gatgttcagc ttcgtcgtac
     2281 cagaaggtgg atgtattgat gaagggctgt ccatccactg aagtggcaaa gtgacccact
     2341 ttgaaagtat tgatattttc gcgaatccac gcatcgtctt tggtgaggtg cggtctgcgc
     2401 tgaaaagcag tgggattctg accgatgtat tctcttggca tgaggttctc ctattgattg
     2461 tctacgagta gatagcgata ccaggtccac tgagcgatgt agccaaagat aacgctgaca
     2521 atgactgctg accatgtgac ccaatttgaa atgccgatat aggtcatgag gaaatagatg
     2581 ccagcgccgg tagtgagcgc gctgagcaaa gccacttcca ctgccatgag attggcaatg
     2641 ctatatggct tgttggtggg tggaatctgt cgctttcgcc atttgaaggc gggctccaac
     2701 tcgggcatat gtttgatgta ataatcctta atttgattca tcgcttctac tgcttcatgc
     2761 cacgcggcgc gcagacgggc aagctgggca atggtcaacg cacccatgaa agttagaatg
     2821 gtgaagagga agaaaaaagc taaagagatg gtgctttgat ctggctcgaa gcgtgtgccc
     2881 agaatggcgg cgacaaaaga gccaaccgaa acgaagtaaa aagaggtcac ccgcgagcgg
     2941 tcttcattcg attgaaaaac gttctgggca atatattgat attctgagtt gagaatttca
     3001 tcggtgttta gtgcgggttt acgtggcatg caattgctcc ttgatgaagg tacttctatt
     3061 atataggctt atcacagatg caaaaagcgg acgtttcatc aacgtccgct tttgttttta
     3121 cgtattacgc ttagaaagcc cattcacctt tacgcatgac agcttcgcgc gttccatcgg
     3181 cccggatgcc atcaatgtcc atttggtcag acccgatcat aaagtccaca tggatgaggc
     3241 tgtcattgcc gccagccgct atgaattctt ctttggtcat tgatgtgccg cccttgacgt
     3301 tgtagcgata agcggtaccc aaagcaaggt ggctggacgc attttcatcg aagagggtgt
     3361 tatagaagag gtgctttctc tgagagatgg gagagctatc aggaaccaaa gcagcctctc
     3421 cgaagcgtga ggcgttttcg tctgtttcta aaagtttgtg gatgttttct tcgccttgtt
     3481 tggctgtgac cttgttcaca cgtccatttt cgaaggtgag cgtgaattcg tcgatcatgg
     3541 taccagagag aatcagcgga cggctggaag tgacatagcc ttctgttttt tcacgatgcg
     3601 gcatggaaaa gacttcttcg gttggcaggt tgggaatgta ttcaataccg ttttgagttt
     3661 tgccagaagc accgatccaa agatggttgt ctggtaaccc aagcgttagg tcggtgccag
     3721 gggctttgta atggagccca gcatagcgct tctcgttcaa gtaatcacat tgacgatgaa
     3781 gtttctgtgc atgttcgcgc catgcttgaa ctgggtctgc ggtattcaca cggcaggcgt
     3841 caaaaatggc ttcccataat ttttcttgtg cttgctctac gggtaagtct ggaaaaactt
     3901 tttgcgccca agcaggggtg gctgtagaaa caacacacca agtttgagta ttttcgtttc
     3961 tatatttggc aacaggttcg tatttttctg aaatcgcttt tcgatatctg ccgatcaaat
     4021 ctgggctgat gccttctagt aaattggggt cttgagaagc gaagctcagg agcgcatctc
     4081 cgcgagagcc atattcgtgc tcgcggtcaa tttgccactg tggaacttca acgaggtgtt
     4141 ccggctttga attttcaaaa cggatgcgcg tcaacctttc atcgatccag cgcacatcaa
     4201 caaaaggaga gcctagttga taagctttct ttgccactaa acgaacaagc tctgcgtcag
     4261 gtaacccggc tgcaatattt aggcgttgtt ctttatgcag gttcaaacca atgtttacaa
     4321 tgatatcggc gtatttttca agagaggttt ggaaggtctc gttcatggtt tctcctttag
     4381 atagacaggg cgttgaatga tttttaccat aagtggactc agatgaccac caactttggt
     4441 gacataaaaa ctccccgcct catgacgggg agttgggttg attcggttac agtgagattg
     4501 cccattcacc ctgacgcatc accggctcat gt
//
LOCUS       DAOQNJ010000162         4529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11868_length_4529_cov_9.513187, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000162 DAOQNJ010000000
VERSION     DAOQNJ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11868_length_4529_cov_9.513187"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1039)
                     /locus_tag="PLG52_06050"
     CDS             complement(137..1039)
                     /locus_tag="PLG52_06050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB41041.1"
                     /translation="MITYRRATPADNFTTFTIFRQSLEDFSKRTGVMAITGGNDPQKM
                     DELWESRRSFWEHLTNTSDNYWIAEKDGEAIGYARSILRGNHRELTEFFVVPGNQSVG
                     VGKELLQRAFPDDVPHRTIIATPDIRAQTRYLKAGVYPFMTEFYFERTPEPQTFETDL
                     VIELAEASPASSDDLGRIDLSILGFRRDIDHQFLTSDRKLYFYKRNGQVVGYGYINRD
                     LYGPFALLEKEDFPAVLAHAENEAHKMGASVVGFEVPTINSSAISHLLNRGYRLEGFL
                     GSIMSEKPFGRFEHYVLTSPPYFL"
     gene            complement(1075..1824)
                     /locus_tag="PLG52_06055"
     CDS             complement(1075..1824)
                     /locus_tag="PLG52_06055"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNB41042.1"
                     /translation="MSHAKFQKYHREVAQALTSIGNPAFGKAVQKDRGSHLKHLGIKF
                     PELRRRVKQGFSFYALPEEQILEIWDALWQTSPYGDVLFAALEYYIPIVKKQVPANLW
                     GVVKGWSLRIDNWCHSDALCSVYSRILEGNQKEVYPQLLEWNQAQSEWLRRISLISLI
                     HYSGKNAVFMPPKKVLPLVSNCLDDERYYVQTAVGWVLREMGQMYQGEIQKYLEIHVE
                     RLSSPAFSRSIERLSTKERTHLQGIRKRRMA"
     gene            complement(1856..3025)
                     /locus_tag="PLG52_06060"
     CDS             complement(1856..3025)
                     /locus_tag="PLG52_06060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB41043.1"
                     /translation="MTQTYDAIVIGAGVMGASLAFHFAERGLKVAILERKVTASGATG
                     HSSGLVRMHYDLAAESHLTFASYKNYFSIWKERVGGECGFQQTGFLQIAKREHEDKLR
                     GNVANQQKIGINTSVISVDEVRKLFPDLVTEYFDYAAYEPDSGYADATLTTNSFLDSA
                     KRNGATLIQNCEVTAIHVSGGKVTGVSTTQGDFSAPIVVNAAGPWAKHVAALAGIEVP
                     LVTWTHDVAFLHRPPTLGKFPAVIDDVINCYFRPEGSALILAAGEDESLRGEAPDAED
                     QTPTPTFLDKLIDQMVQRIPKIEESGLHSIHVGRDGITPDQRAIYSGTDLSGFYLTCG
                     LSGTGFKTSPAAGASMVELILDGTPKTVDITPFRFSRFADGTLLEGEYGYGNIWK"
     gene            complement(3102..3527)
                     /locus_tag="PLG52_06065"
     CDS             complement(3102..3527)
                     /locus_tag="PLG52_06065"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNB41044.1"
                     /translation="MSAKHNPFIKLFWSIHLKLYLWSSGRIGNTIRGLPVLLLTTKGK
                     KTGQLRTKALMYLPYGDAFVVIASNLGKDNHPAWWLNLQADSTATVQVKDARFTVRAR
                     EAEGDEREKLFQALAQKTSDYEQYRSWTSRRIPVVILER"
     gene            complement(3529..>4529)
                     /locus_tag="PLG52_06070"
     CDS             complement(3529..>4529)
                     /locus_tag="PLG52_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNB41045.1"
                     /translation="IFLHACVSASASTPPTETLTPPIPTASLTPSPIPPAPETPQPTV
                     TIPAPQPISRVFIVTFDGLRPDAIDAAGMTNVQEMMKNGAYTLTAQTIMPSTTLPAHS
                     ALVTGTCPAKNTVRWNEYVPQNGYALGTDIFDLTRSVGLRTVLVVGKEKLRQITESTD
                     FFGWVDNTDEEKDKTTLDNMALEQIDLGFGLMMVHFPDGDVVGHEYGWLSKQQLSAYK
                     KNDQSFGRFISAMKKNGYYDGTLFIITSDHGGALTDPPHGTNIPEAMTIPWIISGPQI
                     VPGELKTPVYIFDTAPTVAFALGLPLQAEWDGTPVYEAFGLPVDPLHQGGCPGLTP"
BASE COUNT         1119 a         1073 c         1198 g         1139 t
ORIGIN      
        1 ccccgaagtg tttgagaagt tgtttccttc gtaagtttta ttaatgtcac cctgagcgga
       61 ccgaagggtc tctacctatg ggtcagagac tcttcggtcg cgaagagcac gcttcctcag
      121 agtgacatag cggtactcac aaaaaatagg gcggactggt cagaacgtaa tgttcaaatc
      181 gtccaaatgg tttttcagac atgatcgagc caagaaagcc ttcgaggcgg tatccgcgat
      241 tgagaagatg actgatggca gatgaattga tggtgggtac ttcaaaacct acaacagacg
      301 cacccatctt gtgcgcttca ttttctgcat gagccagcac ggcaggaaaa tcttcctttt
      361 caagcaacgc aaaaggacca tataaatctc ggttgatata cccgtaacct acgacttgtc
      421 cgttacgttt gtagaaatac aacttacggt cactggttag gaattggtgg tcaatgtcgc
      481 ggcggaaacc aaggatcgag aggtcgattc tgcctaagtc gtcggaagag gcgggcgaag
      541 cttctgctaa ttcaataaca aggtctgttt cgaaagtctg cggctcggga gtacgctcaa
      601 aataaaattc ggtcatgaag ggatatacac ccgctttgag ataacgcgtt tgagcgcgga
      661 tatcgggagt ggcaatgatg gtgcgatgcg gcacatcatc ggggaaggcg cgctggagca
      721 attccttgcc cacacccaca gactgattgc ccggcacaac aaagaactcg gtcagctcgc
      781 gatgatttcc gcgcaagatg ctgcgggcgt agccaatggc ttcgccatct ttttcggcga
      841 tccaataatt atcgctggtg ttggtgagat gctcccaaaa actgcgacgg ctttcccaaa
      901 gctcatccat tttttgcggg tcattgccgc cagtgatcgc catcacgcca gtgcgtttac
      961 tgaaatcttc caacgactga cgaaagatag tgaaggtagt gaaattatct gcgggggtgg
     1021 ctctgcggta agtgatcata gttggacatt ttagcagagt ttgattttat gcgatcatgc
     1081 catgcggcgt tttcgaatgc cttgtaagtg tgttcgttct ttcgtgctca accgttcaat
     1141 ggaccttgaa aaagcaggcg aacttaatcg ttctacgtga atttcaagat atttttggat
     1201 ttcgccttga tacatttgcc ccatttctcg caaaacccag ccaaccgccg tttgaacata
     1261 gtagcgttca tcatctaaac aattcgacac caacggcagg acttttttcg gcggcatgaa
     1321 gactgcattt ttgccgctgt aatgaataag gctgatgaga gaaatgcgcc tcaaccattc
     1381 actttgggct tgattccact ctaggagttg gggatagact tctttttgat tgccctctaa
     1441 aatgcgcgaa tacaccgaac aaagcgcatc gctatgacac caattgtcaa tgcgtaaaga
     1501 ccaacctttg acgacgcccc acagattggc tggaacttgt tttttcacta tgggaatata
     1561 gtattcaagc gcggcaaaaa gcacatcgcc atagggggaa gtttgccata gcgcatccca
     1621 aatctctagt atttgttctt ctggcaaagc ataaaaagaa aaaccttgtt tcactctccg
     1681 ccgcaactcg ggaaatttga taccaagatg cttaagatgc gagccgcggt ctttctgcac
     1741 agctttgcca aacgctgggt tgccaatgga tgttagagcc tgcgccactt cgcggtgata
     1801 cttctgaaat tttgcgtggc tcatcaggca atactctcaa tatgtgacaa agaaattact
     1861 tccaaatatt tccataccca tattcgcctt cgagcaaagt cccatcggcg aaacggctaa
     1921 accggaacgg agtgatatcc acagttttgg gtgtgccatc caaaatcaat tccaccatgc
     1981 tagcaccagc agcaggtgac gttttgaaac cagtgccact caaaccacaa gtaagataaa
     2041 acccgctcaa gtctgtgccg ctataaatgg cgcgttggtc gggggtgatg ccatcgcgcc
     2101 caacatggat ggaatgcaag ccgctctctt cgatcttcgg aatgcgctgt accatttgat
     2161 caatgagctt atcaagaaac gtgggtgtgg gtgtttggtc ttcggcatca ggcgcttcgc
     2221 cacgcagtga ttcatcttca ccagcggcaa ggatgagcgc gctgccttcc gggcggaagt
     2281 agcagttgat gacgtcatcg atcacagcgg ggaattttcc caatgtgggc gggcgatgaa
     2341 gaaaggcgac atcgtgagtc catgtcacca atgggacttc gattcctgcc aacgccgcaa
     2401 catgtttcgc ccacggtccc gccgcattga cgacgattgg ggctgagaag tcgccttggg
     2461 tcgtgcttac gccggtcacc tttccgccac tgacatggat ggcggtcact tcgcagtttt
     2521 gaatcagcgt ggcgccgttt cgttttgcag aatcaagaaa agagttggtg gtgagcgtcg
     2581 catcggcata gccggaatcc ggctcataag cagcatagtc aaagtattct gtcacaaggt
     2641 ctggaaaaag tttgcgaact tcatccaccg aaataaccga ggtgttgata ccaatctttt
     2701 gttggttcgc cacattgcca cgcaatttgt cttcatgctc gcgtttggca atttgcaaga
     2761 aaccggtctg ttgaaaacca cattcgccgc ctacgcgctc tttccaaatg ctgaaatagt
     2821 ttttatagct ggcaaaggtt aggtgtgact cagcggcgag atcgtaatgc atgcggacaa
     2881 gcccgctgga gtgcccggtg gcaccagagg ctgtcacctt gcgttcgaga atggcgactt
     2941 tcagtccgcg ctcggcgaag tggaaagcga ggctggcgcc catcacaccg gcaccgatca
     3001 caatggcatc gtaggtttga gtcatagaag agctcctttt gccgcgaatt gtgcggttga
     3061 gttttttggt tgcgaggaat tttagcagag tggggcgatt gttatcgttc caatatcacc
     3121 accggaatac ggcgggacgt ccatgaacga tattgttcgt aatcagaagt tttttgtgcc
     3181 aatgcctgaa aaagtttttc acgctcgtcc ccttcggctt cgcgagcacg aaccgtgaag
     3241 cgtgcatctt tgacctgtac tgttgcggtt gagtctgctt gcaggttaag ccaccaagcg
     3301 gggtgattat ctttcccaag gtttgaggcg atgacaacaa aggcatctcc atatggcaaa
     3361 tacatcaagg cttttgtacg gagttgcccc gttttcttgc ctttggtggt tagaagaagc
     3421 acggggagtc cgcgaatggt attgccaatg cgacctgaac tccacagata aagcttgagg
     3481 tgaatggacc aaaatagctt gataaaaggg ttatgtttgg ctgacatact atggggttaa
     3541 gcctgggcaa ccaccctgat ggagcgggtc tacgggtaag ccaaacgcct cgtaaacggg
     3601 cgtaccgtcc cattcggctt gcaagggcaa gcccagggca aatgccacgg ttggagctgt
     3661 atcgaaaata tagaccggcg tttttaactc accggggaca atttgcggtc cgctaatgat
     3721 ccagggaatg gtcatggctt cgggaatatt tgtgccatgt ggagggtcgg ttaaggcacc
     3781 accatggtca gatgtaatga tgaacagcgt gccgtcataa tatccgttct ttttcatggc
     3841 actgataaaa cgcccaaacg attggtcgtt tttcttgtaa gcgctcaatt gctgtttgga
     3901 aagccagcca tactcatgcc cgaccacatc cccatcgggg aagtgcacca tcatcagccc
     3961 aaaacctagg tcaatctgtt caagcgccat gttatctaag gtggtcttat ctttttcttc
     4021 atcggtgtta tctacccagc cgaagaagtc tgtgctttct gtgatttggc gtagtttttc
     4081 cttgccaacc acgagcacag tccgcaaacc gacagaacgt gtgaggtcga agatatcggt
     4141 gccaagagca taaccatttt gaggaacata ctcattccaa cgaaccgtat ttttagcagg
     4201 acaggtaccg gtcaccaaag cggagtgcgc aggcaaggtc gtgctgggca tgatggtctg
     4261 cgcagtgagc gtgtaagctc cattcttcat catctcttgg acgttggtca tcccggctgc
     4321 atcaatggcg tcggggcgaa gtccatcaaa ggtgacgatg aaaacgcgtg agataggttg
     4381 tggcgcaggg atagtaactg tcggctgggg cgtttcaggc gcaggtggaa ttgggctggg
     4441 tgtcagcgag gcggtaggga ttgggggtgt aagcgtctcg gtcggagggg tagaggcgga
     4501 agcagagaca caggcgtgta gaaagatcg
//
LOCUS       DAOQNJ010000163         4505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11961_length_4505_cov_7.742247, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000163 DAOQNJ010000000
VERSION     DAOQNJ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11961_length_4505_cov_7.742247"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLG52_06075"
     CDS             complement(<1..315)
                     /locus_tag="PLG52_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41046.1"
                     /translation="MATKSTKTTKSTKTTKTTKPRASRAQSTPKVSKPAVRTGTWVAL
                     IAFIAVIATLLYMNRKAETEATATETPAAEETFIFSNESVVSSIEVKPAEGSAVKVER
                     NED"
     gene            complement(319..>1569)
                     /locus_tag="PLG52_06080"
     CDS             complement(319..>1569)
                     /locus_tag="PLG52_06080"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNB41047.1"
                     /translation="TEDKSNTLAPETLQILAELKEPVVATGFFSTNSSTASAEELLAK
                     FKNNSDGKFTYNFVNPDVDPVAAREAGITGDGKIMLQMGETKEIATFATEDELARTLL
                     RLMSPGARTVYFLQGHGEISLESGNELAYSVVQSTLEAKNYTVSTLNLLTTREIPADA
                     LAIIVAGPQKPVSAEEVDLLKAYVDKGGSLVILEDPIFFTKFGDAADPLAEYLVNDWG
                     IVLNNDVVIDGVNQQNPLAAISSLYNPDHPITQNLTENLFVIMPQARSISVSGEKENI
                     TQTWLISTTENSWGETSPLVENENPQFDPATDVQGPLYLALAAENSATTGRVVVFGNS
                     LFAIDGNFDVYGNGNFFINSVDWAAEQEDLLNLTTRPRTERIFTPPVETWRFLLLVLV
                     IVIIIPGMVVFFGVSAWLARRKRG"
     assembly_gap    1570..1669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1753..2496)
                     /locus_tag="PLG52_06085"
     CDS             complement(1753..2496)
                     /locus_tag="PLG52_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41048.1"
                     /translation="MNNIWTIAKREYNNYFNGPLAYVVMLAILLPLGIYFSLILFYSS
                     QQGLYGGSAAPDITPVNGLFAFLMIFLSPALTMRLLSDEARMGTLELLLTAPVRDIEL
                     VVGKWLGAMLFILTLLGTTLIYPLVLNNFVDPGIDWMVVLSSYIALILLCGTMLALGV
                     GISAIFTNQFAAFFVTLGLIFFLYFMIGLPAGLLPQSGEVFNYLSIQNHFDQMNSGTL
                     TLGGIVYNLSLTVFGLFAGFTAIETRRWR"
     gene            complement(2501..3424)
                     /locus_tag="PLG52_06090"
     CDS             complement(2501..3424)
                     /locus_tag="PLG52_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB41049.1"
                     /translation="MIKVSGLTKDYGSRRAIHNLSFDAQQGEIVGFLGPNGAGKTTTM
                     RILTGYMPPTDGEAIVAGYDVVEESLEVRKRVGYLPESVPLYYDMVVSEYLKYMGELR
                     RIPNVDDRVDEVLDMVGLFDRADGYIGNLSKGMKQRVGLAQALLHRPEVLILDEPTIG
                     LDPGQVVEVRELIREIGKERTVLLSTHLLNEAQNLCDRVLIINKGKIVAEDTTENLQA
                     RLLGAERVVIRVRGEAEELADSIKSIKGVRKVETKDDGAVEFEFASGKDLRPEVAKQV
                     IKDGYDLLELRPLGMSLEEIFLELTGNEGKK"
     gene            3622..4341
                     /locus_tag="PLG52_06095"
     CDS             3622..4341
                     /locus_tag="PLG52_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNB41050.1"
                     /translation="MTELLYQTDAYLKEFSAVITSVDAEARAVVLDRSAFYPGGGGQP
                     CDFGTLEVNGVKYSVEKVKKQGDDVFHFIGGTEPLPTPGSASLGTLDWVRRHKLMRTH
                     TALHILCGTVFRDYGAKVTGGDMEPLKGRMDFEFETMRAELVREIEAAVNAEVKKNHE
                     IRVKILPREEAFQIPDLIRTKINLLPEGIMQVRTVEIVGLDLQADGGTHVANTSEVGT
                     IKVVEYKSKGAINKRLYIEIE"
BASE COUNT         1108 a         1060 c         1160 g         1077 t
ORIGIN      
        1 atcttcgttt cgttcaacct taactgcgct accttcagca ggcttgactt caatactact
       61 gacaacgctc tcgtttgaga agatgaaagt ctcttcagcc gccggggttt ctgtagcggt
      121 agcttctgtt tctgccttgc ggttcatgta cagtaaagtg gcaatcacag cgatgaaagc
      181 gatcagcgca acccatgttc cggtgcggac ggcgggttta gagacctttg gggtgctttg
      241 cgcacgagac gcgcgtggtt tggtggtctt ggttgttttt gtagattttg ttgttttggt
      301 cgatttcgta gccatgaatt aacctcgttt acgacgggca agccatgcag acacgccaaa
      361 gaaaacaacc ataccgggga tgataatcac gatcacgagg accaagagca ggaagcgcca
      421 ggtttcaacc ggaggtgtga agatgcgttc ggtgcgtgga cgggtggtga ggttcaggag
      481 gtcttcctgt tcggcagccc aatctacaga attgatgaag aagttgccgt tgccatacac
      541 atcgaagttt ccgtcgatag caaaaagtga gttaccgaac acaaccacac gaccggtcgt
      601 cgcagaattc tccgcagcaa gggcgaggta caaaggacct tgaacgtcag ttgcggggtc
      661 gaattgtgga ttctcgtttt ctaccaaggg agaggtttcg ccccaggagt tttctgtggt
      721 gctgatgagc caagtttggg tgatattttc tttttcacca gaaacactga tgctgcgagc
      781 ttgtggcatg atgacgaaaa ggttttcggt caggttttgg gtgatggggt gatcgggatt
      841 gtacaaagaa gagatcgccg ccagcggatt ttgctggttg acaccatcga tcacaacgtc
      901 attattgaga acaatgcccc aatcattgac gaggtattca gccaaggggt ctgcggcatc
      961 accaaacttg gtgaagaaga tcgggtcttc gagaatgacc aaggaaccgc ccttatccac
     1021 ataggctttg agcaagtcca cttcttctgc gctgacaggt ttttgcggac ccgcaacgat
     1081 aattgccaaa gcgtctgcag ggatttcgcg agtcgtcaac agattaagcg tgctgacggt
     1141 gtaattcttt gcctcaaggg tactttgcac aacgctatag gctaattcgt tgccagattc
     1201 aaggctgatc tcgccatgtc cctgcaagaa atagaccgta cgtgcgccgg ggctcataag
     1261 gcgcaaaagg gtgcgcgcaa gttcatcttc tgtagcaaag gtagcgattt cttttgtttc
     1321 gcccatttgc aacatgatct tgccgtcgcc tgtaatacca gcttcgcgag cggcaacggg
     1381 gtcaacatct gggttcacga agttgtaggt aaacttgccg tcgctgttat tcttgaattt
     1441 agcgagcaat tcctctgcgc tggctgtgct tgaatttgtc gagaaaaagc ctgtagctac
     1501 gacaggttct tttaattcag ccaaaatttg cagggtttct ggagcaaggg tattgctctt
     1561 gtcttcggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ccggtcgaaa
     1681 tacatgcgac cagagaaact gccagcgcga tgactgcgta tcttgcgttt ggatttttct
     1741 ttttcccagc cattatcgcc atctccgagt ttcaattgcg gtaaaaccgg cgaagagacc
     1801 gaacaccgtc aggctcaagt tatagacgat gccgccgagt gttagtgtgc cggaattcat
     1861 ttggtcaaag tgattttgaa tgctcaagta gttgaagact tcaccgcttt gggggagcaa
     1921 ccctgcaggc agaccgatca tgaagtacaa gaagaagatc aagccgaggg tcacgaagaa
     1981 ggcagcaaac tggttcgtga aaatggcaga gatgccaacc cccaaagcca gcatggtacc
     2041 gcaaagcagg atcaaagcga tataagaaga aagcaccacc atccagtcga tgccggggtc
     2101 aacgaagttg ttcagcacca gcggatagat caaggtagtg ccaagcaagg tgaggatgaa
     2161 caacatggcg cccagccact tgccaaccac cagctcaata tcgcggacgg gggcggtcag
     2221 caaaagttcg agagttccca tacgagcttc gtcagagagc aaacgcatag taagcgccgg
     2281 gctgaggaaa atcataagga aagcgaacaa cccgttgacg ggggtgatat cgggggcagc
     2341 gctgccgcca tataaacctt gctggctgct gtagaaaagg atcaaactga agtaaatgcc
     2401 aagtggcaaa aggatcgcca gcatcacaac ataggcgagc ggtccgttaa agtagttgtt
     2461 gtattcgcgt tttgcgatgg tccaaatatt gttcatggga ttatttcttc ccttcgtttc
     2521 cagtgagttc gaggaagatt tcttcgaggc tcatgcccaa cgggcggagt tccaaaaggt
     2581 cgtacccatc tttaataacc tgtttggcaa cttcggggcg taaatccttg ccggaggcaa
     2641 actcgaattc cacagcaccg tcgtctttgg tttccacctt gcggacaccc ttaatgctct
     2701 tgatcgagtc tgcaagctct tcggcttcgc cacgtacacg gatcacaacc cgttcggcac
     2761 cgagcaagcg agcctgcaag ttttcggtgg tgtcttcggc gacgatcttg cccttgttga
     2821 tgatcaagac gcggtcgcag aggttttgcg cttcattcaa aaggtgagtg gagaggagca
     2881 ccgtgcgctc tttaccaatc tcgcggatta attcgcggac ttctaccacc tgcccagggt
     2941 cgagaccaat tgtcggctcg tcgaggatga gcacttcggg gcggtgcaaa agcgcctgag
     3001 ccaagcccac acgctgtttc atacccttgg ataggttgcc aatgtacccg tcggcacggt
     3061 caaacaagcc gaccatatcc aacacttcat ccacacggtc atccacattc gggatgcggc
     3121 gaagttcgcc catatacttt aagtattcag aaacgaccat gtcgtagtac agcggcacag
     3181 actccggcaa atagccgacg cgcttgcgta cttccaacga ttcttccacc acatcataac
     3241 cagctacaat cgcttcgcca tcagtaggcg gcatgtaacc agtcaggatg cgcatggtgg
     3301 tggttttccc ggcgccattg ggacccaaaa agcccacaat ctcgccctgt tgggcatcaa
     3361 aactcagatt gtggatggcg cgacgtgacc catagtcttt tgtgagacca ctaactttga
     3421 tcattgcttc tcctcacggt gaaataatat ataaaatggg attatcgcaa agaaaagaca
     3481 cagccgtgtt ggcggtgcct tttttttcgt accgcagaat actatacaat atatgaaaga
     3541 ccaaagtcaa gcgggtgtat agccctctaa tccaatccta attttgtctg accaattcat
     3601 tccatcttgt ataattgagc catgaccgaa ctcctctacc aaacggatgc ctatctcaaa
     3661 gagttttcag ccgtcatcac atctgtcgat gctgaagctc gtgctgtcgt tctcgaccgt
     3721 tctgcttttt atcccggcgg gggaggtcag ccctgtgatt ttggcacgct ggaagtcaat
     3781 ggcgtgaaat actcagtgga aaaagttaag aaacagggag atgacgtctt tcactttatc
     3841 ggtggaaccg aacctttgcc aacgcccggc tccgcctctc tcgggacctt agactgggtg
     3901 cggcgacata agctgatgcg cactcatacc gctctgcaca tcttatgcgg aacggtcttc
     3961 cgcgattatg gtgccaaggt tactggcggt gatatggagc cgctcaaagg gcgtatggac
     4021 tttgaatttg agaccatgcg cgcagaactc gtccgcgaga ttgaggcggc ggttaatgcc
     4081 gaggtgaaga aaaaccatga gatcagagtc aagatcctcc cgagggaaga ggcgtttcaa
     4141 atcccagatt tgattcgcac caagatcaac ctcctcccag aaggaatcat gcaagtgcgc
     4201 acggtcgaga ttgtaggctt agatttgcaa gccgacggcg gaacgcatgt ggcgaacacc
     4261 tctgaagttg ggaccatcaa agtggttgaa tataagagta aaggcgcgat caataagcgg
     4321 ctttatattg aaattgaata gcccttttag gggagggaag ttcgtaatag tttaggtata
     4381 atgtcgacac ttggcggggc tacctgccta catttatatt atgaggagaa tgccaaaatg
     4441 aaagcgaaaa aactgtcgat cttgctggct gcaattatgt tagttgctgt tcagctcgcc
     4501 tgcgc
//
LOCUS       DAOQNJ010000164         4503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_11970_length_4503_cov_9.236061, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000164 DAOQNJ010000000
VERSION     DAOQNJ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11970_length_4503_cov_9.236061"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLG52_06100"
     CDS             <1..417
                     /locus_tag="PLG52_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41051.1"
                     /translation="TIKERYPQLNIKFAPKDWEALLIILRQRSFETAQSIDRFQADSI
                     MLRNIAFGLLLMTLANVIAFFTTMSTFYLLLAFAELFACWMLASESFKFRTWFFTQIY
                     EGSLIYGTSIEEVISHGKGQVSKSKGQKQTSTKIKL"
     gene            complement(490..1884)
                     /locus_tag="PLG52_06105"
     CDS             complement(490..1884)
                     /locus_tag="PLG52_06105"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNB41052.1"
                     /translation="MQWQTNPYFAPIIISSLIAIVCAFFVITRRNRASGSTALLGLIF
                     SVIIWGFAYAMELASVSLKWQIFWAKVEYLGIPFVPLFFFIFAYQFTQSTSKLSRRVI
                     FFLVVISSIFVLLAWTNEFHNLIWTELGQKNIDGYYMLTLGHGMAFWLLITYSYILLA
                     IASISIIRRALSGPAEIRKQTYIILVGASVTWLGNIIYISGLSPVPELDLTPIGFVIS
                     SVIFSFGIFGYGMLDIMPIAGEFILESLDDVVIVTNPKGMLIYVNRAFEYYFSNPVQS
                     LIGRQATEAFSNLPVLKSLFDSSNTIRREITVTPENRPLFIFNVRTFNIRLSANEVIG
                     RVTILNDITERRAAENRLSQQSLEAGSGDLPLMVMYRVSDDKIVDVNRTFLINLGYER
                     KDVLGRTLMGVQFWGVNQRADFLRMLYKNGSLKDYAFEIGKNGQKYILSASQIEMQDT
                     KYIVIFAQEDWSGS"
     gene            complement(2143..2547)
                     /locus_tag="PLG52_06110"
     CDS             complement(2143..2547)
                     /locus_tag="PLG52_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41053.1"
                     /translation="MKKFNIKSLLVFVVALIMVLSLSACATEEVSDASATDEAATEVA
                     TEVATEVATEEAGVIVPEFTFNNTGEEEICEIYLSPVGQDQWGPDQLGGATIPSGGSY
                     VLQNIPAGQYDVKAVGCAGSEVIGQLDIQPQQ"
     gene            2756..4018
                     /locus_tag="PLG52_06115"
     CDS             2756..4018
                     /locus_tag="PLG52_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB41054.1"
                     /translation="MPTTDMLKRDVIIIGGGPVGLSCAYYLLKSGRQVTLLDAKEIGK
                     GSGSGNAGHIVPSHIIPLAAPGVVTSALKWMLDPAHSPFGMKVSLNPNYLMWLLRFTI
                     ACSQNNVDRSLEPLNQLGQLSAKNFAQIIAEEKFDCAYQEMGLLHLFKTEKAFDEGQH
                     EGDFMQRHGIPVSVYDKNKIHEVEPAAVDDVLGGVHFTGDAHLNPAVFLNLLKERVKS
                     MGVELMDGTQVTGFETANGKITKVKTSAGDFEAEQIVLAAGALTPSVAKDLKLNIPVQ
                     PARGYSMTMSATKTMPSHALILGERRIAVSPMAGFLRFTGRLEVGNYSMEPNPIWIQR
                     IETSAREYLRLDEKLDVKETWAGLRPTTPDGVPIIGKSPKHNNLVLATGHAMLGLSLG
                     PGTGAVVSQLLNGQETAFSLSPMRLERF"
     gene            4097..>4503
                     /locus_tag="PLG52_06120"
     CDS             4097..>4503
                     /locus_tag="PLG52_06120"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNB41055.1"
                     /translation="MTKVKVLWFLSVALLSACSNAEPSPTSTPEPTTTPTSTPIPMPS
                     QVSEINLPDDPMNAPYGIAVNSLGNLYVNDAGNNRVLIFDADGNLLSKWDTQGSGDGE
                     FKTLGFGSLAIDADDNVFIVDNGNSRIQKFDKDG"
BASE COUNT         1238 a          962 c         1123 g         1180 t
ORIGIN      
        1 acgatcaaag aaagatatcc ccaacttaat attaagtttg cgccaaaaga ttgggaagca
       61 ctgttgatca ttcttcgtca acgcagtttt gaaaccgcac aaagcattga ccgttttcaa
      121 gccgacagca ttatgttgag gaacattgcc tttgggcttt tgctgatgac gcttgcgaat
      181 gtgatcgctt tcttcacgac catgagcacg ttttatttgc tgcttgcttt tgctgaactc
      241 tttgcttgct ggatgttggc ttctgagagc tttaagttcc gcacttggtt ctttactcaa
      301 atctatgaag gttctttgat ctatggcacg agtatcgaag aggtcatctc gcatgggaaa
      361 gggcaagttt caaaaagtaa gggtcaaaaa caaaccagta ccaaaataaa gctgtagttt
      421 attggataaa ggaataaaaa agaggcaatt tggtatcacc aaattgcctc ttggtcttta
      481 agtgggaaat tacgacccag accaatcttc ctgagcgaag atcacaatgt acttcgtatc
      541 ttgcatttcg atctggctgg cagaaaggat atatttttgt ccgttcttcc cgatctcaaa
      601 tgcataatct ttgagagagc cgttcttata cagcatgcgt aaaaagtcgg ctcgttggtt
      661 tacgccccag aattgaacgc ccatcaaggt gcgcccaagg acatctttgc gttcgtatcc
      721 gaggttgatt aagaaggtgc ggtttacatc tacgatctta tcgtcactga cgcgatacat
      781 caccatcaag ggcaaatccc cagaccctgc ttccaggctt tgttgtgaaa gccggttttc
      841 tgcggcgcgg cgttcggtaa tatcgttcaa aatggtcact cgcccaatca cctcgttggc
      901 gctgaggcgg atgttaaacg tccgcacatt gaagatgaac agcggacgat tttcaggcgt
      961 gaccgtaatt tctctgcgaa tggtatttga gctatcaaat agagatttca gaacgggtaa
     1021 gttggaaaaa gcttcggtag cttgcctgcc gatcagggat tgtacggggt tgctgaagta
     1081 atattcaaag gcacggttga catagatcaa cattcccttg gggttggtga cgataactac
     1141 atcatccaag ctttcgagaa tgaactctcc ggcgatgggc ataatgtcta gcatgccgta
     1201 cccaaagatg ccgaagctga agatgacact ggagatgacg aagccgatcg gggttaagtc
     1261 taattcgggc accgggctca aaccggaaat gtaaataata ttccccaacc atgtgacgct
     1321 tgcgccgacc aaaatgatat aggtttgctt cctaatttca gcgggtccgc ttaaggctct
     1381 acgaatgatg ctgatcgaag caatagccaa tagaatatac gagtaggtga ttagaagcca
     1441 gaacgccatg ccatgcccaa gcgtcagcat ataatagcca tcgatatttt tttgaccgag
     1501 ttcggtccag atcaaattgt ggaattcgtt agtccatgcc agaaggacga atatggagga
     1561 aataacgacc agaaagaaaa taacgcggcg cgataatttg ctggtgcttt gcgtgaattg
     1621 ataagcaaat atgaaaaaga ataaaggcac aaaaggaatg ccaaggtatt ccacctttgc
     1681 ccagaatatc tgccatttca ggctgacact ggcaagctcc atggcgtagg caaatcccca
     1741 aatgatgacg gaaaagatca acccgaggag cgctgtcgag ccagatgccc ggtttctgcg
     1801 cgtgatgaca aaaaatgcac agacgatggc gatcaggctg gaaatgataa ttggagcaaa
     1861 atatgggtta gtttgccatt gcattggaac gttttcagac ctttgaaaag ttaattttag
     1921 aaacaccctt tggctgaaat gtttcaaaga aattcagtga attattcatg ggttcgtggg
     1981 tttgtcaaga cagggttatg caattgcaag aaacctttac aacccatccc atgttttaga
     2041 gcgccgctca ttataatggc atatagcgag tgaatttaga gcggatatgt cataaataaa
     2101 aagacctctg agaaaaactc agaggtcttt tctgtcgttc aattattgct ggggttgaat
     2161 gtcgagctga ccgatcactt cggagccagc gcagccaacg gctttgacat catactgacc
     2221 agcggggatg ttctggagaa cataggaacc accggagggg atggtggcgc caccgagctg
     2281 atcgggaccc cattgatcct gaccgacagg ggagaggtag atttcgcaga tttcttcttc
     2341 gccagtgtta ttaaaggtga attcaggaac gatcacacct gcttcttcag tagcgacctc
     2401 ggtagccact tcagtggcaa cttcggtggc agcttcatca gtagcggaag catcgctgac
     2461 ttcttcagtt gcgcatgcag aaaggctgag aaccatgatg agggcgacga cgaacacgag
     2521 caaagacttg atgttgaact tcttcattgt attcctttat gttgtttaga ttacgtgcgg
     2581 gcgcggatta tacataaaaa agaaaaaagt gcaacctatt tttgattata aaattattaa
     2641 tttggaatct ttttaatggt tctcatattt ggattcggtc aacatagact caagcggctc
     2701 tggaatataa tgacttgaaa tattcaaact tgaatgatct ttggagaaga taacgatgcc
     2761 tacaacagat atgttgaaac gcgatgtgat tatcatcggt gggggacccg tggggttaag
     2821 ttgtgcgtat taccttttga aatcgggcag gcaagtgact ctgctggatg caaaagagat
     2881 tggcaaaggc agtggttcag gcaatgccgg gcatatcgta ccgagtcaca tcattccgct
     2941 cgccgcaccc ggtgtggtga cctctgccct taaatggatg cttgacccgg cgcatagtcc
     3001 ctttgggatg aaagttagcc tcaacccaaa ttacttgatg tggctattgc gttttacgat
     3061 agcgtgtagc cagaacaatg tggatcgctc attggagccg ttgaatcaac tcggtcaact
     3121 tagcgcgaag aattttgctc agatcattgc cgaagaaaaa tttgattgtg cctatcaaga
     3181 gatgggacta ctgcatttat tcaagaccga gaaagccttc gatgagggtc agcacgaagg
     3241 tgatttcatg cagagacatg gcattcctgt cagtgtatat gacaaaaaca aaattcatga
     3301 agtcgagccc gccgccgtgg acgatgtcct tggcggtgtg catttcacgg gagatgcgca
     3361 cctaaacccg gcggtatttc tcaacttgct gaaggaacgt gtcaaatcta tgggtgtgga
     3421 gttgatggac ggtacgcagg tcacgggctt tgaaactgca aatggcaaaa ttacgaaagt
     3481 caaaacgtct gctggagatt ttgaagcaga acagatcgtg cttgccgctg gcgcgctgac
     3541 tccttccgtt gccaaagatt tgaagttgaa cattccggtt cagcctgcac gtggatacag
     3601 tatgaccatg tccgcaacca agaccatgcc gagccatgcg ttgattctgg gcgaacgccg
     3661 catcgctgtc tcaccgatgg ctggattttt gcgcttcaca ggtcggctcg aagtgggcaa
     3721 ttacagtatg gaaccgaatc cgatttggat tcaacgcatc gagacttcgg cgcgagagta
     3781 tctgcgtttg gatgaaaaac tggacgtcaa agaaacatgg gcgggcttgc gtcccaccac
     3841 gcccgatggc gtgccgatca ttggcaagtc accgaagcat aacaatcttg tgctggcaac
     3901 gggtcatgcc atgcttgggc tctcgttggg acccggaacc ggcgcggtcg taagtcaact
     3961 gctcaatggg caggaaacag ccttcagttt aagtccgatg cggttggaac ggttttagtc
     4021 aaaaccattc tagaggatgg tgtcatcttg cttttgtgta tctataatcc tataaatctt
     4081 atgaggagta gagtacatga ccaaggtgaa agtcttgtgg ttcttaagtg ttgctttatt
     4141 gtccgcttgt agtaatgcag agccttctcc aacgagtacg cctgaaccaa caaccacccc
     4201 tacgagtaca cccataccca tgccttctca agtttccgaa ataaacctgc ctgatgaccc
     4261 aatgaatgca ccgtatggaa ttgccgtaaa ttcgcttggc aatttgtacg tcaacgatgc
     4321 tggtaataac cgtgtgttga tttttgatgc agatggaaac ctcttatcca aatgggacac
     4381 acaaggtagc ggcgacggcg aatttaagac cttgggcttt ggtagtttgg caattgatgc
     4441 ggatgataat gtcttcatag tggataatgg caatagccgc attcagaaat ttgataaaga
     4501 tgg
//
LOCUS       DAOQNJ010000165         4482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12079_length_4482_cov_7.668173, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000165 DAOQNJ010000000
VERSION     DAOQNJ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12079_length_4482_cov_7.668173"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLG52_06125"
     CDS             <1..528
                     /locus_tag="PLG52_06125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB41056.1"
                     /translation="IGSFIIATERLSDELAHELSPKNRMIFDYKHFLNYFRLWDNRMI
                     FGGRAAFFPENENTIAQSGEILRNEMIMVYPQLKDVNVDYVWGGTLDFAFDQMTHVGE
                     EDGIFYSLGYAGHGVAMATYLGATVAEAMLKGNIKEHPFAKFDFPSAPMGLYNGNPWF
                     LPFAGMYYKILDWVE"
     gene            complement(501..1847)
                     /locus_tag="PLG52_06130"
     CDS             complement(501..1847)
                     /locus_tag="PLG52_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB41057.1"
                     /translation="MPSLFNRLFRKEKSTSHKIVAIKVGVVVNQRYQLESELGRGGMG
                     IIYKAHDLTKNREVAFKVINPETANSLSLNQFAREADILSSFDHPHIVSVHETGFVNE
                     DPSLPFLVMELLHRKPLSEIGALTYSRIVSIAKQICEALAYIHSQGFVYRDIKPNNIL
                     LKKRGFDYFVKLIDFGLARPLGDAYLPNESSLAGTVFYLAPELIEGQVADVRSDLYAL
                     GILLYEMIVGRVPFSDVDEATIQLQHQQQKASPPSQSRLGVPMELDTLVLRLLEKNRQ
                     DRPASANEVCEILNSIRFDKKTQGNLPSNISDRSDAQPIIQLLAEHSLVTLISNDVSL
                     ALAAVSHLSEQFPDGVWVVNLEAVSEPVAVLPMVCSMFGVKEIQNRPLAVTLIEFLRE
                     KNLLLLFTHCGRVSGACAQLASAITVSCPDVQIMAVSDKPLNLPNEKIYSTQSKIL"
     gene            1930..2661
                     /locus_tag="PLG52_06135"
     CDS             1930..2661
                     /locus_tag="PLG52_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNB41058.1"
                     /translation="MRILVVSDIHANFVAFEAVLKDAGSFDTVWCLGDVVGYGPQPNE
                     CVEQLRELHPVCLAGNHDLAVTGQVALWDFTKDAQEVIFWTRHWLTQENMEWLTTLSS
                     TPQVEHGITIVHASLRDPVWEYITNASLVKDNIQFAQTPICLHGHTHLATLFRKKWDE
                     YRILEEYPRLNTPIKLIPDQMFINPGSVGQPRDEDPRAAYALIDTDEMTYTQKRVLYD
                     VTTVQGLMKQAKFSNRLIRRLRFGQ"
     gene            2679..3116
                     /locus_tag="PLG52_06140"
     CDS             2679..3116
                     /locus_tag="PLG52_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB41059.1"
                     /translation="MYKKILVPLDGSTVAEGVLPHAKSLAYSEGAELILLTVAANPAM
                     DYLFSDPGIAETAVQEQVEKSKAYISTIENDLHSAGFKVSTLMRVGSVAEVILSVAEE
                     LQVDAIAMSTHGRTGAARWLLGSVAERVVHNSNVPVLLIRVKE"
     gene            3553..>4482
                     /locus_tag="PLG52_06145"
     CDS             3553..>4482
                     /locus_tag="PLG52_06145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB41060.1"
                     /translation="MSQQSVTASADQEEAQSLPRIIFASAAGTMIEWYDFYIFGSLAT
                     VIASKFYQTGTDIGDIIAWLATFAIGFIVRPFGAIVFGRVGDLIGRKYTFLVTMTLMG
                     LCTFAVGLLPTIEVLGSLAGVLLITLRILQGLALGGEYGGAATYVAEHSPHGRRGFYT
                     SWIQITATLGLFMSLGVILATRKIVGEDAFKEFGWRIPFLISILLVAVSLWIRYSLRE
                     SPMYTKLKKSGGISKNPLKESFGNPYNLGFVGLALFGATMGQGVVWYTGQFYALFYLQ
                     KIFGVDLVDSNMIIGGALLFATPFFVFFGNLSDK"
BASE COUNT         1151 a         1006 c         1105 g         1220 t
ORIGIN      
        1 atcgggtctt ttattattgc aacggaaaga ctctcggatg aattggcgca tgaactcagc
       61 ccgaagaacc gaatgatctt tgattacaaa catttcctga attattttcg tttgtgggat
      121 aaccgcatga tctttggcgg acgggcggcg ttcttccccg aaaatgaaaa tacgattgct
      181 caaagcggcg agatcttgcg aaatgagatg ataatggttt acccgcagtt gaaggatgta
      241 aacgtggact atgtgtgggg cggcacgctc gattttgcat tcgaccaaat gacgcatgta
      301 ggcgaagaag atgggatctt ttactcgcta ggctacgccg ggcatggtgt ggcaatggcg
      361 acctatttgg gcgctacggt tgccgaagcc atgctaaagg gaaatatcaa agagcacccg
      421 tttgcgaagt ttgatttccc ctctgcgcca atgggattgt ataacggcaa cccgtggttc
      481 ctgccgtttg ccggaatgta ttacaagatt ttggattggg tggaataaat tttttcgttg
      541 ggtaaattca acggcttgtc actaactgcc atgatttgaa catcggggca agaaactgtg
      601 atggcagatg ccagctgagc gcatgcccca gagacacgcc cgcaatgcgt gaaaaggagt
      661 agaagatttt tctcgcgcag aaattcgatg agcgtgaccg caagcggacg attttgaatc
      721 tcttttacgc caaacattga acagaccatt ggaagaacag cgacaggttc acttactgct
      781 tccaagttaa caacccaaac accatctgga aattgttccg aaagatgtga tacagcagct
      841 aacgcaagcg acacgtcatt gctaatgaga gtcacaagtg aatgttccgc aagcagttgg
      901 ataatgggtt gagcatctga acggtcagaa atattggatg gcaaattccc ttgagtcttt
      961 ttatcaaagc gaatcgaatt caagatctca catacttcat ttgcggatgc cgggcgatct
     1021 tgccgatttt tctcaagcag acgcaaaaca agcgtatcta attccatcgg cacgcctaag
     1081 cgagactggc tgggtggaga tgctttctgc tgctgatgtt ggagttgaat tgtggcttcg
     1141 tccacatccg aaaacggcac acgcccgacg atcatttcat acagcaagat acccagcgcg
     1201 tataagtcag accgcacatc agcgacctga ccttcaatca attcgggggc gaggtaaaac
     1261 acggttccgg caaggctgct ttcgttcggc agatatgcat cccctagcgg tcgagctagc
     1321 ccaaaatcaa tcagttttac gaaataatca aatccgcgtt ttttgagcag gatgttattc
     1381 ggttttatat cacgatacac aaagccctga ctgtgaatat atgccaacgc ttcacatatc
     1441 tgttttgcga ttgagacaat gcgggaatag gtcaatgcgc caatttcaga aaggggtttt
     1501 cggtgcaaga gttccatcac aagaaaaggc agtgatgggt cttcattgac aaaccccgtc
     1561 tcatgcactg aaacaatatg gggatgatcg aagctagaaa gaatatccgc ttcacgcgca
     1621 aattggttca gcgaaagtga atttgcagtt tcggggttga tgactttgaa ggcaacttcg
     1681 cggttcttag ttaaatcatg agccttataa ataatgccca taccaccacg cccaagctca
     1741 ctctccaatt ggtagcgttg gttgacaaca actccaacct taatagcaac tatcttgtgc
     1801 gaagtggatt tctccttgcg aaagagacgg ttaaaaagag agggcatgtt caaattatag
     1861 cgttgctgtc atctgcaaaa aagttacttt cgtcattctt tctgtacgct ataatatttt
     1921 taggtatcca tgcgcattct tgttgtatca gatatccatg cgaacttcgt cgcgtttgaa
     1981 gcggtgttga aagatgccgg tagttttgat acagtctggt gcttgggcga tgtggtggga
     2041 tatggtccgc aaccgaacga atgtgtagag caactgcgag aattacaccc agtttgtctg
     2101 gcgggcaatc atgaccttgc cgtaacaggt caagtcgcgt tatgggattt cactaaagat
     2161 gcacaagagg tcattttttg gacgcgtcac tggctcacac aagaaaatat ggaatggctg
     2221 acaaccttgt catctacgcc tcaagttgag cacggcatta ccatcgtcca cgccagcttg
     2281 cgcgacccgg tctgggaata catcaccaat gccagcttgg tcaaagacaa tatccaattc
     2341 gctcaaaccc ctatctgttt acatggacat actcatcttg ctactctctt tcggaagaaa
     2401 tgggatgaat atcgcattct ggaggaatat ccgcgcctca acacacccat caaattgata
     2461 cccgaccaaa tgtttatcaa ccctggaagc gtaggtcaac cacgcgatga agatccgcgt
     2521 gccgcttatg ccctaatcga tacagatgag atgacctaca ctcaaaagcg agttctttac
     2581 gatgtgacaa cagttcaagg gctgatgaaa caggcaaagt tttcgaatcg gctcatacga
     2641 cgtctacgtt tcgggcaata aaccgatagg aggaagttat gtacaaaaag attctggttc
     2701 cactcgatgg ctcgactgtt gcagagggag tgcttcccca tgcaaagtca ctggcatatt
     2761 cagaaggcgc agagctgatt ctgttgaccg tcgccgccaa cccagccatg gactatctct
     2821 tttctgaccc tggcattgca gaaaccgcgg ttcaagagca ggttgagaaa agcaaagcat
     2881 acatttcaac cattgagaac gaccttcatt ctgccgggtt caaggtctca accttgatgc
     2941 gggtcggctc ggtagcggag gtgattctta gcgtggcaga agaattacaa gtggatgcga
     3001 ttgccatgtc cacgcatggg cggacgggtg cggcgcgttg gcttctgggc agtgtggcag
     3061 aacgggttgt gcataattcg aacgtgccag ttctattgat tcgtgtcaaa gagtaggtgg
     3121 ggtgagctaa cggggaaact aattttttga gtgtaatgcc cggcaagagc ggggaaagag
     3181 ccgttattct ttcggacatt ttttgtctga aattcaattt ttatgttgcc atatgacaat
     3241 catcaaaaaa atccatgaca tttgacgttg aaatcgacat aacgaattcg gcaaacttag
     3301 tgttatttcc tcaaatatgt aagcccttta gtggttaggt ggatcaaggt ttttgttcga
     3361 ggtaacaatg cccgaaagaa tcttttcccc aatacgcggt ttgatatgcg cttcttctgc
     3421 tcgtccatgg ggtaatggat gttctcggag taaaggagcg ctgatgactt attctgccta
     3481 gtgatcaccc agcctgttac aaccgcgatg agcggaacat atccatttat cttcccaaag
     3541 gagaactgca ttatgtctca acagtctgta acagcctccg ctgaccaaga agaggcgcaa
     3601 agtctacccc gtattatttt tgcatctgct gccggtacca tgatcgaatg gtacgatttt
     3661 tatatttttg gcagtctggc aaccgtgatc gccagtaagt tctaccagac cggtactgac
     3721 attggcgata ttattgcctg gctggcaact tttgcgattg gttttattgt gcgcccgttt
     3781 ggcgcgattg tatttggacg tgttggtgat cttattgggc ggaaatatac ctttcttgtt
     3841 accatgacct tgatgggtct gtgtacattt gcggtcggtt tattgcccac cattgaagtt
     3901 cttggttcat tggctggtgt tcttttgatc acattacgca ttttgcaagg gctggcgttg
     3961 ggcggcgaat atggcggcgc agcaacttat gtagctgaac attcaccgca tggcaggcgc
     4021 ggcttctaca caagctggat tcaaatcact gcgacattgg ggttgttcat gtctcttggt
     4081 gtgatcctcg ccacgcgcaa gatcgtgggc gaagatgcct tcaaagaatt tggctggcgc
     4141 atcccattcc ttatttcaat ccttttggtg gctgtttctc tttggatccg ctactcgcta
     4201 cgcgaatctc cgatgtacac caagctcaag aaatcgggcg gcatttccaa gaatccactc
     4261 aaggaaagct ttggtaaccc ctacaattta ggctttgtgg gcttagccct ctttggcgcc
     4321 acaatgggac aaggcgttgt ctggtatacc gggcaattct atgcgctgtt ctacctgcaa
     4381 aaaatattcg gcgtggacct ggtcgattcg aatatgatca tcggcggtgc gttgcttttt
     4441 gccactccat tttttgtctt ctttgggaat ttatccgaca ag
//
LOCUS       DAOQNJ010000166         4470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12137_length_4470_cov_7.936580, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000166 DAOQNJ010000000
VERSION     DAOQNJ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12137_length_4470_cov_7.936580"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLG52_06150"
     CDS             <1..694
                     /locus_tag="PLG52_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB41061.1"
                     /translation="NTGIQRLRDIIDDMIDVSLIDNQLLTLNIQPMWTTHLLNLVRNE
                     FKKPIAERNQTLNVYDFDGSDLMIYGDSERLYQALQNVVSNAIKYTPDKGIITIDGRL
                     LPGFIEITVKDTGIGISPENQSLIFDKFGQLGRTDLHSSGKTKFKGGGPGLGLSITRG
                     IIEAHGGTIWVESTGYDEVQLPGSIFHILLPTRTEPNNPVMLKFFGNNETKMTETESS
                     GKETPPTDNPSA"
     gene            654..2024
                     /gene="hflX"
                     /locus_tag="PLG52_06155"
     CDS             654..2024
                     /gene="hflX"
                     /locus_tag="PLG52_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNB41062.1"
                     /translation="MAKKLPQPTTPPRERAFLVGADLHDNHSILSLEDSLAELALLAD
                     TAGVDVVGELTQKLNRPHVETYIGPGKVEELKALAEETLCEVVIFDDELLPRHQRELE
                     KALGKNIRVIDRTALILDIFAQHAHTKEGMLQVELAQYEYNLPRLTRAWTHLERQAGG
                     GGGRSGSTGGVGLRGPGETQLEVDRRAIRRRITHIKKELEKVEEHRTRYRAQRRRTRI
                     PTVALVGYTNAGKSTLLNRLAKADVYVADQLFATLDPTTRRVQVSGENTVLFTDTVGF
                     IQKLPTALVVAFHATLEEIAEADLLLHVVDISHPNALGQYQAVLETLKEIDADHIPMI
                     TALNKMDQLRDPQNAERVLENFPKSVAISALKGTGIDNLLMLVQEELFEAFEPIGVRL
                     PYKEGSLISLFHELGQVERVEHERGGVIIHGSIPGRLLAQFAPWKIKPNGNTSHTLEE
                     MDLEEE"
     gene            2021..2224
                     /locus_tag="PLG52_06160"
     CDS             2021..2224
                     /locus_tag="PLG52_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41063.1"
                     /translation="MISKLLDDLSNYLAHRKGLIPIIGLLLIILNLILQFIFPGSLIV
                     TTNLFLHIGLIIAIFGLMLAWAL"
     gene            2252..4243
                     /locus_tag="PLG52_06165"
     CDS             2252..4243
                     /locus_tag="PLG52_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB41064.1"
                     /translation="MSTSSVTIKTLLNQYRIEEFITLTPFGDLYRAIDERNGKSVALT
                     ILSKAIASDPETLKELEANNVRLQTLHHPNLNKHLGLQKTPEQAFLLEDWVDGPSLKD
                     LRGKGRLGANEALFITKYVCAGLEVLHKQNLLHLHLAPEFIRINKRGEVVLCGLAGAK
                     PVNTKAEIKFSKYQAFYKSPEQLREEKLTTAADIYSLAVILYELTTSFWINGKAAPKT
                     SDAIRKTHLDVTPPAPASINHNLPDNFSRMILWALRKNPEDRLKTTTELASSLALGAQ
                     TSLDKVPLHTTPEFAPVISAALNTWTFLPPPKPTSVSADAIPLEERLAAISGPKPKPT
                     RRLGVVPVLLFLVLAGFISLFFFIRPAAETVLPTPIVYTSIAVNYTPPPTSTITPRPT
                     LTNGGRIVFTCTRGEYNQLCMINSDGSDLAQLTDMEASNYYPAFTPDSSSVLFASNRA
                     GPNFDFFVLNFTEREVFQLTVNVGNVISPDFSPDGRAIVFANRVGDGPTAIWMVNAEG
                     LNPRPVYTGTGDIVSVSWSPNGEKIAYAMNTGTPQEYDIFTMDTNGRNHLKISQGLQG
                     IGGSVDWSPDGTSLLVYAGPFGDKDIFKIDAATGAFAQLTDGGNNAGASYSPDGQYIV
                     FNSLRNGDQADLYIMEADGSGLRQLTNDPEPDWGANWIE"
BASE COUNT         1192 a         1192 c          997 g         1089 t
ORIGIN      
        1 caacactggc attcagcgtt tgcgcgacat tattgacgac atgatcgatg tctcgttgat
       61 cgacaaccaa ctgctcaccc tcaacatcca acctatgtgg acgacccatc tgctaaacct
      121 tgtccgcaat gagttcaaga agcccatcgc cgaacgtaac cagaccctga acgtttacga
      181 cttcgacggc agcgatttga tgatctacgg cgattcagaa cgcttgtatc aagccttaca
      241 aaatgtcgtc tccaacgcca ttaagtacac acctgataaa ggcattatca cgattgacgg
      301 acgattactt cccggcttta ttgagatcac cgtcaaagat accggtattg ggatctcacc
      361 agaaaaccag tctttgatct ttgataagtt cggtcagctc gggcggacgg acctacactc
      421 gagtggtaag accaagttca aaggcggagg acccggtctt ggtctatcca tcacccgcgg
      481 cattattgaa gcacatggcg gcaccatctg ggttgaatcg acaggatacg acgaagtaca
      541 attaccgggt tccatttttc atatcctgct cccgacccgc accgaaccga acaacccggt
      601 gatgcttaag ttttttggta ataacgaaac gaaaatgaca gaaacggaat caagtggcaa
      661 agaaactccc ccaaccgaca acccctccgc gtgaacgcgc ctttttagtg ggcgcagatc
      721 tgcacgataa tcattccatc ctttctctcg aagactctct tgctgaactt gccttgctgg
      781 cagatactgc cggtgtagac gtggtcggtg aactgactca aaaactcaac cgtccgcacg
      841 ttgagacgta tatcggtccc ggcaaggtgg aagaactcaa agcgctcgca gaagaaactt
      901 tgtgtgaagt ggttattttc gatgatgaac ttctgccacg tcaccaacgc gaattggaaa
      961 aggcgcttgg caaaaacatc cgcgtcattg accgaacggc tctgatcctc gatatctttg
     1021 cacaacatgc gcacacaaaa gaaggcatgt tgcaagtgga gcttgctcaa tacgaataca
     1081 acttgccacg cctcacccgc gcatggacac atcttgaacg tcaggcaggt ggtggcggcg
     1141 gacgttccgg ctcgacaggt ggcgtgggtt tgcgcggtcc tggtgaaacg cagctcgagg
     1201 tagatcgccg tgctattcgt agacgcatca cccatatcaa aaaagaattg gaaaaggtgg
     1261 aagagcatcg cacacgttat cgcgctcaac gtcgacgcac ccgcatcccc accgtcgcct
     1321 tggttgggta caccaacgca ggcaaatcta cgctgttgaa tcgcctcgcc aaagcagacg
     1381 tgtacgtggc agatcaatta ttcgccaccc tcgaccccac aacacgccgt gtgcaagtct
     1441 ctggtgaaaa taccgtattg ttcaccgaca cagtgggctt cattcaaaaa ctgcctaccg
     1501 cgttggtggt tgcctttcac gccaccctcg aagaaatcgc cgaagcagat ctgctcttac
     1561 acgttgtgga tatttctcat cccaacgccc ttgggcaata tcaagctgtg ctcgaaacct
     1621 tgaaagagat cgatgccgat catatcccca tgatcaccgc attgaacaaa atggaccagt
     1681 tgagagaccc acaaaacgct gaacgtgttt tggaaaactt tccgaagtct gtggctatct
     1741 ccgcactgaa aggcaccggc atagataact tactcatgct ggtacaggaa gaattatttg
     1801 aagcttttga gccgattggt gtgaggttgc cctataaaga aggttctctc atttctttat
     1861 tccacgagtt aggtcaagtg gaacgcgtag agcatgaacg cggtggtgtc attattcatg
     1921 gcagcattcc cggcaggctt ttagcacaat tcgcaccgtg gaagataaag cccaatggca
     1981 atacctcaca cactttagaa gaaatggatt tggaggaaga atgatctcaa aattactcga
     2041 tgatctttcc aattatcttg cccatcgcaa agggttaatt cccattattg ggttgctttt
     2101 gatcatcctc aatctgatct tacaatttat ttttcccggt tctctaatcg tcaccacaaa
     2161 ccttttcctg catatcgggt tgattattgc catcttcggt ttgatgttgg cttgggcgct
     2221 ctaaacctac acctgatcgc gacgtacttc aatgtcaacc tcttccgtta cgatcaaaac
     2281 cctgctcaac caatatcgaa ttgaagagtt cattacctta actccctttg gagatttata
     2341 tcgtgccatc gacgagcgca acgggaagtc cgttgcgctt acgattctat ccaaagcaat
     2401 tgcaagcgac ccggaaacac tcaaagaact tgaagccaac aatgttcgtt tacaaacttt
     2461 gcaccaccca aacctcaaca aacatctggg tctgcaaaag actcccgaac aagcatttct
     2521 gctggaagat tgggtggatg gaccttcact aaaagacctg cgtggcaaag gcaggctggg
     2581 tgcaaacgaa gccttgttca tcacaaaata tgtctgcgcc gggctcgaag tacttcacaa
     2641 acagaaccta ttgcacttgc atcttgcacc agagttcatt cgcatcaaca aacgcggtga
     2701 ggtcgttctc tgcgggttgg caggcgcaaa accggtcaat accaaagccg aaatcaagtt
     2761 cagcaaatat caggcgttct ataaatcacc cgaacaactg cgcgaagaaa aactcaccac
     2821 cgccgccgat atctactcac tcgccgtcat cctttacgaa ctaacaacct cattctggat
     2881 caacggcaaa gccgctccga aaaccagtga tgccattcgc aaaacacatc tggacgtgac
     2941 accacctgcc cctgccagca tcaaccacaa cctacctgat aacttttcac gcatgatcct
     3001 ttgggcgttg cgcaaaaacc cagaagacag actcaaaacg acgactgaat tagcatcttc
     3061 acttgcatta ggggcgcaaa cttcattgga taaagttccg cttcatacta cgcccgaatt
     3121 tgctcccgtt atctcagccg ctctcaacac gtggaccttt cttccgccac ccaagcccac
     3181 ctccgtcagc gcagatgcca ttccactaga agaacgcctt gccgccatct ctggtccaaa
     3241 gccgaaacct actcgcaggc ttggtgtcgt tcccgtttta cttttccttg tcctagcagg
     3301 gtttatttct ctgttttttt tcatccgccc tgccgcagag actgtcctcc ctacgcctat
     3361 tgtttataca tccattgctg tgaactacac cccgccgccc acatccacaa tcacaccgcg
     3421 ccccacgttg accaatggtg ggcgcatcgt tttcacctgc acacgcggcg aatacaacca
     3481 actctgcatg atcaacagcg atggcagcga cctcgcccaa ctcaccgata tggaagccag
     3541 caactattat ccagctttca caccagatag cagttcggtg ttgtttgcct ccaaccgcgc
     3601 cggaccgaat tttgatttct ttgtattgaa tttcaccgaa cgcgaagtat ttcagctcac
     3661 cgtaaatgtt ggcaatgtga tttcgcccga tttctctcct gatgggcgtg cgattgtttt
     3721 tgccaatcgc gtcggagacg gtccaactgc gatttggatg gtcaacgccg aagggctgaa
     3781 tccacgcccg gtgtatacag ggacgggaga tatcgtctcg gtatcatggt cgcccaatgg
     3841 tgaaaaaatc gcctatgcca tgaacaccgg cacccctcaa gaatatgata tttttacgat
     3901 ggacaccaac ggtagaaatc atctaaaaat ctcgcaaggg ttgcaaggca ttggcggcag
     3961 tgtggattgg tcacctgatg gaacgtctct actcgtgtac gcaggtccgt ttggagataa
     4021 agatattttc aagatcgatg ctgcgacggg agcgttcgcg caattgaccg atggtggcaa
     4081 taatgcgggc gcatcctact caccagatgg gcaatacatt gtctttaatt ctctacgcaa
     4141 tggcgaccaa gctgatcttt atattatgga agcagacggt tcgggtttac gtcagctaac
     4201 gaatgacccc gaaccggatt ggggtgcgaa ctggatcgag taagaaaaaa atttttgggg
     4261 aaagaagagt ggaatggtta gaatccagcg agagcgggga atgaagcgtg aatcaagttt
     4321 tgaatccgct ataacatttt tgcatccagc aagttcgtga tgtatcgatt ctgtattgca
     4381 gaggaagttg caataacgat tcattaacag aatcggcata atctgaacgg ttaaaaacct
     4441 ttacatgcag gttatttgat gtatcattca
//
LOCUS       DAOQNJ010000167         4467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12150_length_4467_cov_6.444923, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000167 DAOQNJ010000000
VERSION     DAOQNJ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12150_length_4467_cov_6.444923"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLG52_06170"
     CDS             <1..229
                     /locus_tag="PLG52_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNB41065.1"
                     /translation="THLHKMAAPMLVIQGRNDPRVVAAESEDLVRELKASGKPIELLV
                     FEDEGHDVLKYNNRVTCYNAIADFFAKHLNP"
     gene            233..511
                     /locus_tag="PLG52_06175"
     CDS             233..511
                     /locus_tag="PLG52_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41066.1"
                     /translation="MNLPAILFALVVALLAGALFHSIRGGGGWRLLLHLALSAAGFAL
                     GQGISIWLGYILLQFGVLDIGMGVIGSLVFLGIGDWLSRIKPRNESSV"
     gene            560..1192
                     /locus_tag="PLG52_06180"
     CDS             560..1192
                     /locus_tag="PLG52_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNB41067.1"
                     /translation="MEFLKSAIDLFLHLDEYLQTIITTYGAWTYGILFVVIFIETGLV
                     VMPFLPGDSLLFAAGTFAALGSFNVWGLVGLLAVAAVLGDAVNYSIGHYLGDRAYNIK
                     WIKKEYLEQTHAFFEKHGGKAIFLARFVPIVRTFAPFVAGIGKMSYSYFATYNIVGGI
                     SWVTIFTLLGFFFGNIPFVKKNFELVIIAIILISVVPMIYEWWKARRAAK"
     gene            1246..1905
                     /locus_tag="PLG52_06185"
     CDS             1246..1905
                     /locus_tag="PLG52_06185"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HNB41068.1"
                     /translation="MNHIELSSKEESLLNQETLAEKMELVVEKLPADEVTLVEIMDIV
                     GADSLMLVTIFMSLIFLVPVSIPGVSTVFGTGILLIGITHLFARKLWLPKPIANRKLS
                     APKLKEAFKRAIVWLHRLEKVSRPHRLSKLTAEGGMTIFNNLSFILAALLLMAPFGFI
                     PFSNTLPALALIFFALGMMERDGGSVIFGHIANIATIIYFGFLIAGGGWSIGEFIKVL
                     G"
     gene            1950..2312
                     /locus_tag="PLG52_06190"
     CDS             1950..2312
                     /locus_tag="PLG52_06190"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNB41069.1"
                     /translation="MKYLAILFTLFILAIIILADKNALPPFIHAIYDFPNGDKAGHFI
                     LFGLLNFILTLTFLRALPNRTRSRVALSVGLILALAITLEEFSQRYFSSRTFDLIDLT
                     ASYIGVLVGGWIAFRIKK"
     gene            complement(2345..>4467)
                     /locus_tag="PLG52_06195"
     CDS             complement(2345..>4467)
                     /locus_tag="PLG52_06195"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNB41070.1"
                     /translation="NAYLKDHSGTALSLEYAVLGLLSPDYVIEPWEPVNSLTWGKAMA
                     WDLRGNMGEEIERAELLKTLTPEQVALLYPEYPQDHPVIVNKIGEGGQSASTSQPSSF
                     DYSALNLDSLSHNASLLDPVLGPLGDGIGSNSWAVSGALTDTGKPYLANDPHLGIQMP
                     SIWYQVGLHCVEKTDSCPFEVAGFSFAGVPGVVIGHNDKLAWGFTNVGPDVMDLYIEK
                     VNPENPNQYEVNGEWVDFETYEETINIVGGEPVTITVKKTRHGPVISDTYGPTKNVGD
                     PEDTKFIPFKDRVGGVELPEQYVIALAWTALTPSTPFEAIWGFNKAQNWEDFRKAASD
                     FHVPAQNLLYADVEGNIGYQMPGDIPIRKNGDGTVPVPGWTDDYEWEGFIPFDELPYA
                     LNPEEGYIVTANNQVNPWDYPYLITKDWDYGYRAQRIVDMIKNAPGKIDKEYIQSMQG
                     DATDLNAESIVPVWKEIDYKAATENEAYALDQLQNWDYTCKADSKACAIFQWFWWNLD
                     QNTINDDVPERNAVSGGSKDFETFRHLLPETDNFFWDDKLTTDVTETRDDIFIASLTE
                     TVQQLEKEYGKDPAKWPRWGELHTATFRNGTLGESGVFLIEDLFNRGPFETNGGQSLV
                     NATGWDLGTSFEVDWLPSMRMIVDFNDLNASLTVHTTGQSGHAYHPHYDDMALFWADV
                     SYYPMWWDQESVIDDAEGHLVLKP"
BASE COUNT         1042 a         1026 c         1053 g         1346 t
ORIGIN      
        1 gacgcatctg cacaaaatgg cggcgccgat gctcgttatt caaggacgca atgatccgcg
       61 tgtagtggct gcagagtcgg aggatttggt ccgcgagttg aaagcaagtg gcaaacccat
      121 cgaattgctg gtctttgaag atgaaggtca cgatgtgctg aaatacaaca atcgggtgac
      181 ttgctataac gccatcgccg atttctttgc gaagcatttg aatccataaa ctatgaatct
      241 gccagccatt ttgttcgcat tggttgtggc attgttggca ggcgccttgt tccattccat
      301 tcgtggcggg ggcggatggc gcctgctgct tcaccttgct ctgagtgcag ccggatttgc
      361 actcgggcag ggcatcagca tctggcttgg gtatatcttg cttcaattcg gagtgttaga
      421 tattgggatg ggagtaatcg gtagcttggt ctttcttggc atcggtgact ggctgagccg
      481 cattaaacca cgcaatgaaa gcagtgtata atctccgccc gttcgttcaa aaaacatcat
      541 gaaagcgagt aaaaaataaa tggaattcct aaaaagtgcg atcgaccttt ttttacatct
      601 cgatgaatat ttgcaaacta ttatcactac ctatggcgcc tggacgtatg gcattctttt
      661 tgtagtgatc tttatcgaaa cgggcttggt tgtgatgccc ttcctgccag gagactccct
      721 gctctttgct gcagggacct ttgctgcact cggctctttc aatgtgtggg gcttggtcgg
      781 gttgttggca gttgctgcgg tattgggcga tgcggtcaat tactccatcg gtcattatct
      841 tggtgaccgt gcctataaca tcaaatggat taagaaagag tatttggaac aaacccatgc
      901 ctttttcgag aagcatggtg gtaaagctat tttccttgcg cgctttgttc ctatcgttcg
      961 tacatttgct ccttttgtgg ctggtattgg caaaatgtct tactcttatt ttgccaccta
     1021 taacattgtg ggcggcatta gttgggtgac gatctttact ttactcggct ttttctttgg
     1081 caatatcccc tttgtgaaaa agaattttga gttggtcatt attgccatca ttttgatctc
     1141 ggttgtgccg atgatttacg aatggtggaa ggcacgtcgc gccgcgaaat aaactgtagt
     1201 gttctgaaat cactttttac tttagaaaat actgaggata ttaaattgaa ccacattgaa
     1261 ttgtcttcca aggaagagtc tttgctaaat caagagactc ttgccgaaaa aatggagttg
     1321 gttgttgaaa aactccccgc agatgaagtg actttggtcg agatcatgga tattgtcggt
     1381 gctgatagcc tcatgttagt aactatcttc atgtccttga tctttttggt gccggtttcc
     1441 attcctggtg ttagtacggt tttcggaacg ggtattctcc tgatcggtat tactcatctt
     1501 tttgcacgaa agctttggct acccaaaccc attgcgaatc gtaaactctc agcacccaaa
     1561 ttgaaagagg cattcaaacg tgctattgtt tggttgcatc gtcttgaaaa ggtcagccgt
     1621 ccacatcgct tgagcaaact cacggcagaa ggtggcatga ccattttcaa taacttgagc
     1681 ttcattcttg ccgctcttct gctcatggcg ccttttgggt tcattccgtt tagcaacacc
     1741 cttcctgcct tggcattgat cttttttgca cttggcatga tggaacgaga tggcggctcc
     1801 gtgatttttg ggcatatcgc caatattgcc acgatcatct atttcggctt cctcattgca
     1861 ggtggcggat ggtctattgg cgaattcatt aaagtgcttg gttaactctt tataaggcag
     1921 gctgggttcc aagcccagcc tgttttttta tgaaatatct agctattctt tttacactgt
     1981 tcattcttgc gatcatcatt cttgcagata aaaatgcttt accaccattt attcatgcaa
     2041 tttatgattt tccaaatggc gataaagccg ggcacttcat cctttttgga ctcctcaact
     2101 ttattctcac tcttaccttt ctccgcgcgc ttcccaaccg gacccgtagc cgggttgctc
     2161 tttcagtcgg tttgatactc gccctcgcca tcaccctcga agaattctcg caacgttact
     2221 tttcctctcg taccttcgat ttgattgatc ttacagcaag ttatataggt gttttagtag
     2281 gcgggtggat cgccttccga ataaaaaagt aggtcacgcc tcaagcgtga cctacttttt
     2341 tattttaggg cttcaacacc aaatgccctt ccgcatcatc aatgacggat tcttgatccc
     2401 accacatggg gtagtaagag acatctgccc agaatagcgc catatcatcg taatgcggat
     2461 gatatgcatg cccggactgc cctgttgtat gaaccgtaag agacgcattg aggtcattaa
     2521 aatccacgat catacgcatg gatggcaacc aatctacttc gaaggatgtg cccaagtccc
     2581 agccagttgc gttgactagg ctttgaccgc cgttggtctc gaaaggtccg cgattgaaga
     2641 ggtcttcaat caagaagaca ccagattcgc caagtgtccc atttctaaac gtggcagtgt
     2701 gtaactcacc ccagcgcggc catttggcgg ggtctttccc gtattctttt tctaattgct
     2761 gaacagtctc agttaacgat gcaatgaaaa tatcatcgcg agtttcagtc acatcggttg
     2821 taagtttatc gtcccagaag aagttatctg tttctggcaa aagatgacgg aaggtttcga
     2881 aatcttttga gccgccgctg acagcattgc gttcgggcac atcatcgttg atcgtattct
     2941 ggtcaagatt ccaccagaac cattggaaaa ttgcgcaggc tttagagtca gctttacagg
     3001 tgtaatccca attctgtaac tggtcgagtg cataggcttc gttttcggtg gcggctttgt
     3061 aatcgatctc tttccaaaca ggaacgatgc tttcggcgtt taggtctgtt gcatcaccct
     3121 gcatagattg aatgtattct ttatcaatct tgccgggggc attcttgatc atgtctacaa
     3181 tgcgttgagc acgataacca tagtcccaat ctttggtgat gaggtatgga taatcccatg
     3241 ggttgacttg attgtttgcg gtgacgatat acccttcttc agggttgagc gcgtagggca
     3301 attcatcgaa cggaataaaa ccttcccatt cgtaatcatc cgtccaacca ggaactggca
     3361 cagttccgtc accattcttt cgaattggaa tatcgcccgg catttgataa ccgatgtttc
     3421 cttctacatc ggcatacagc aagttctgag caggcacatg gaagtcgctc gcggctttgc
     3481 ggaagtcttc ccagttttgt gccttgttaa aaccccaaat ggcttcaaat ggtgtggatg
     3541 gggttaaagc cgtccatgcg agggcgatca cgtattgttc gggcagttct actccgccta
     3601 cacggtcttt gaatggaatg aacttcgtgt cttctgggtc gccaacattt ttagttggac
     3661 cgtaagtgtc tgagatgacc ggtccgtggc gggtcttttt taccgtgata gttaccggtt
     3721 caccaccgac aatgttgatg gtttcttcgt atgtttcaaa atcaacccat tcgccgttga
     3781 cctcatattg attggggttt tctgggttga ccttttcgat gtacagatcc atcacatctg
     3841 gtcctacatt cgtaaagccc catgcgagtt tgtcgttatg accgattact acaccgggaa
     3901 ccccagcaaa tgagaagcca gctacttcga acggacagct atctgttttt tcgacgcaat
     3961 gcaaaccgac ctgataccaa atagacggca tttgaatgcc aagatgcggg tcattggcga
     4021 gataaggttt gccggtgtcg gtcaatgcgc cggagacagc ccaagagttt gacccaattc
     4081 catcgccaag cggaccaagt accgggtcca gtaatgaggc gttgtgtgag agtgaatcaa
     4141 gattgagggc agaatagtca aagcttgaag gttgactagt tgatgcagat tgtccacctt
     4201 caccaatttt gttcacgatg acaggatgat cttgaggata ttctgggtag agcaatgcaa
     4261 cctgttctgg cgtgagagtt tttaacaatt ctgcacgttc gatttcttca cccatgttgc
     4321 cacggagatc ccatgccatc gctttacccc atgttaacga attgacaggt tcccacggtt
     4381 caatgacgta atcggggctg agcaacccca acacggcata ttccaagctg agggcggttc
     4441 cgctatgatc tttgagataa gcgttca
//
LOCUS       DAOQNJ010000168         4466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12153_length_4466_cov_8.042167, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000168 DAOQNJ010000000
VERSION     DAOQNJ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12153_length_4466_cov_8.042167"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLG52_06200"
     CDS             <1..645
                     /locus_tag="PLG52_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB41071.1"
                     /translation="AAISSGYKDKTRTFTEKDWSIIENEIGAYAKSKTLAERAAWDFI
                     NGPENSNHMELVAINPPLILGPVPNKNFRTSVEVIRTYMLGQVPGVGRIKMGLVDVRD
                     VAAAIILAMATPEAAGQRFLCSGGMAWLKEIAGVLHAEFSPRGYKIPTLEFPSWAVRF
                     LGLFDKKIARVTEELDRDTEQSSEKAKRILKWSPRPLKEAIIAMGNSLIEHGFV"
     gene            710..1699
                     /locus_tag="PLG52_06205"
     CDS             710..1699
                     /locus_tag="PLG52_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB41072.1"
                     /translation="MNYLPSTSRYQSMHYRRSGRSGLKLPAVSLGLWHNFGGVDVYEN
                     SRAMVLRAFDLGITHFDLANNYGPPPGSAEETFGQIMAKDLHPWRDELFISSKAGYYM
                     WEGPYGEWGSRKYLVSSLDQSLKRMGLEYVDLFYHHRPDPDTPLEETMQALDFIVRSG
                     RALYVGISNYPADKAREASQILKSLGTPCLIHQPKYSMFERWVEDGLLQTLKEEGIGC
                     IAFSPLAQGLLTDKYLGGIPEGSRASKAHGFLKPAHITDERIAKVQKLNTLAQQRGQT
                     LAQMALAWVLRHEGMTSVLIGASKVSQIEDAVGMLNKVEFSGDELQAIENILR"
     gene            complement(1756..3447)
                     /locus_tag="PLG52_06210"
     CDS             complement(1756..3447)
                     /locus_tag="PLG52_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNB41073.1"
                     /translation="MIQITEPTPNVEVHLPNGKTLTGPRGTKVGEFLAQVRDDFPAPI
                     VAAIINNQIHELTYPIMVESACIPVTMDTADGARIYRRSLIFLLEAAFSKTFPQGYLN
                     IDHSVSSGGYYCEVNGRDALSQQELEGLSQTMQALVKDDLPFERREVPIQEAIQYFTQ
                     HGHEDKLRLLKHRHKEYLTMYSVNGLMDYMHGYMVPSTGHLKWFGLKKVNGGFILQYP
                     RRHAPTQLAPFGDYPKLIRAFRQYGDWLETLHIDSVGVLNDAIQAGRADEIVLVSEAL
                     HEQGIADTAQQIAEKNSRIILIAGPSSSGKTTSSRRLAVQLLARGISPYPLELDNYFI
                     DREKTPRDENGEHDFETIESLNLERLARDIEKLISGEEVQLPRYNFKTGRSEEGDVVR
                     LREGQPLILEGIHGLNPRLIPDRWADSAFRLYVSALTQLNLDRHNRVSTTDTRLIRRI
                     VRDARERGYSAQATISRWESVRRGEKRHIFPYQENADVMFNSALVYELSALKPLVEPL
                     LRQVPYDATEYIEARRLLAFLDWFLPLDANLIPMNSIVREFIGGSSLKDFKIWRG"
     gene            complement(3496..>4466)
                     /locus_tag="PLG52_06215"
     CDS             complement(3496..>4466)
                     /locus_tag="PLG52_06215"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNB41074.1"
                     /translation="VEKPKMVEELKAKGLRLDLTVDERRNTKDVSIIDSKSFIIEPSL
                     EDALKYGPFDAALFALKSFDTVSAMEGLKPFADKLPPLLCLSNGVDNEPIIARTLGPD
                     KVIYGTVTTAIGRRGPGSIVLEKLRGVGIAAGHPLSEKLVAAFDNAYLKCRLYPDALS
                     MKWSKMLTNLVANPTSAILNMTAAEVFADKRLYKIEIDMLKECLAVMASMNLEVTDLP
                     GTPVRALAFATKLPLWLSKPFLSKAAGSGRGGKMPSFHIDLYSGRPQSEVEFLHGAVV
                     REGKTRGIPTPVNKVLTETLLALTNKEILLEEFAGKPEKLLSKLRY"
BASE COUNT         1160 a         1082 c         1119 g         1105 t
ORIGIN      
        1 gctgccattt cttcagggta taaagacaaa accagaactt tcaccgaaaa agattggtcg
       61 atcatcgaaa atgagattgg tgcgtatgcc aaaagcaaga ccctcgccga acgcgccgca
      121 tgggatttta tcaacggacc tgagaacagc aatcacatgg aattggtcgc catcaacccg
      181 ccgctaatcc ttggaccggt gccgaataaa aatttccgca cgtccgtaga agtgatccgc
      241 acctacatgc tggggcaagt ccctggtgtg gggcgcatca aaatggggtt ggtagatgtg
      301 cgcgatgtcg cggctgctat tatcctagcc atggccacgc ctgaagccgc agggcagcgt
      361 ttcttatgtt caggcggcat ggcatggcta aaagagattg caggtgtttt gcatgctgaa
      421 ttctcgccgc gcggatataa aatccctacc cttgaatttc cgagttgggc tgtgcgcttt
      481 ttaggcttgt tcgataaaaa gatcgcgcgc gtcaccgaag aattagaccg cgatacagaa
      541 caatcgagtg aaaaagcgaa acgcattctc aaatggagtc cgcgaccatt gaaagaggca
      601 attatcgcta tgggaaatag cttgatcgag cacgggtttg tataaaattt taagatgagg
      661 tgacagttac tttaaaagtg actgtcacct ttatataata ggagccttta tgaattacct
      721 cccttccacc tctcgttatc aatccatgca ctaccgccgc tcagggcgta gcggactaaa
      781 actcccggcc gtttcgcttg gtttgtggca taacttcggc ggcgtggacg tgtacgaaaa
      841 cagccgcgcc atggtgctgc gcgccttcga cctcggtatc actcactttg accttgccaa
      901 taattatggt ccgcctcccg gctcggcaga agagaccttt gggcagatca tggcaaaaga
      961 cctgcatccc tggcgcgatg aattatttat ctcatcgaaa gccgggtatt acatgtggga
     1021 aggtccatac ggagaatggg gttcgcgcaa atatctcgtc tccagccttg atcaaagcct
     1081 caaacgcatg ggactggaat atgtggactt gttctatcat catcgccccg accctgatac
     1141 tccgcttgaa gaaacgatgc aagcgctcga tttcatcgtc cgcagtggac gggcgttgta
     1201 tgtgggcatt tcaaattacc ctgccgataa agcccgtgaa gcgtctcaaa ttctcaagtc
     1261 cttaggcacg ccttgtttga ttcatcagcc caaatacagc atgttcgagc gctgggtgga
     1321 agatggtctg cttcagacct tgaaagaaga aggcatcggc tgtattgctt tctctccgtt
     1381 ggcacaaggc ttacttactg ataaatatct cggcggcatt cctgaagggt cacgcgcttc
     1441 gaaggcgcat ggctttctca aaccggctca catcaccgat gaaagaatag ccaaggttca
     1501 aaagctcaac actctcgccc agcaacgcgg acaaaccctc gcacaaatgg cgctggcttg
     1561 ggtgctgcgc catgaaggca tgacctccgt gttgatcggt gcaagcaagg tcagccagat
     1621 cgaagatgcc gttgggatgt tgaataaagt ggaattttca ggggacgagt tgcaagcaat
     1681 cgagaatatc ttaaggtaaa gaattaaaca aaaagacctc cgaggttttg aaaacctcgg
     1741 aggtctgtat ttaaatcacc cacgccaaat cttgaaatct ttcaaactgg agccgccgat
     1801 aaattcacgc acgatggaat tcatcgggat caagttcgca tccaaaggca ggaaccaatc
     1861 gaggaaggcg agcaaacggc gggcttcaat gtattcagtg gcatcatatg gcacttgccg
     1921 caacaacggc tccaccaatg gcttcaacgc cgaaagctca tacaccaacg cagaattgaa
     1981 catcacatcg gcattttctt gatatgggaa aatatggcgc ttctcgccgc gccgcaccga
     2041 ctcccagcgc gagatcgtgg cttgggcaga atatccgcgt tcacgagcat cgcgcacaat
     2101 acggcgaatc aatcttgtat cggtagtgga aacgcggtta tggcggtcta ggttaagctg
     2161 ggtcagggca gagacgtaaa gccgaaatgc tgaatcagcc cagcggtcgg ggatgaggcg
     2221 cgggttcaat ccatggatac cttcgagaat gagcggttga ccctctcgca gccggaccac
     2281 atctccttct tcgcttcttc cggtcttgaa gttgtagcgc ggaagttgaa cttcttcgcc
     2341 gctgataagt ttttcaatgt cgcgagccag ccgttcaaga tttaatgatt caatggtttc
     2401 aaaatcatgc tccccatttt cgtcacgtgg agttttttca cggtctataa aataattatc
     2461 caactcaagc gggtacggtg agatgccgcg cgccagcaat tgcacagcca ggcggcgcga
     2521 agatgtggtc ttgccagaag aggatggacc cgcgattaaa atgatgcgtg aatttttttc
     2581 tgcaatctgt tgagccgtat cggcaatacc ttgttcatgt aacgcttctg agaccagcac
     2641 aatttcatcg gcacgcccgg cttgaatggc atcgttcaaa acgccaacac tgtcaatgtg
     2701 caaagtttca agccaatcgc catattgacg aaaggcacgg atcaatttcg gataatcgcc
     2761 aaagggggca agctgtgtcg gtgcatggcg gcgcggatat tgcaagataa acccgccgtt
     2821 gaccttcttg agcccgaacc atttgagatg cccagtagaa ggaaccatgt atccatgcat
     2881 gtaatccatg agcccgttaa cagagtacat ggtgagatat tctttatggc gatgcttaag
     2941 caagcgcaac ttgtcttcat gcccatgctg tgtaaagtat tggatggctt cttgaatggg
     3001 aacttcgcga cgctcaaatg gaaggtcatc tttaaccaag gcttgcatgg tttgcgaaag
     3061 accttccaat tcttgctgtg aaagcgcatc acgcccgttg acttcacaat agtatccgcc
     3121 agaggagacc gaatggtcga tgttcaaata tccttgcgga aatgttttag agaaagctgc
     3181 ttccaaaagg aagattaatg aacgccggta aatgcgggca ccatcggctg tatccatagt
     3241 gacaggaata caagctgatt cgaccatgat gggataggtc aattcatgaa tctggttatt
     3301 gatgatggca gccacaatcg gcgcagggaa atcatcgcgg acctgtgcaa ggaactcccc
     3361 cactttcgtc cctcgtggac cggttaaggt tttgccatta ggcaaatgga cttcgacgtt
     3421 tggagttggt tcggtgattt gaatcatgga agatcaaatg tcaaaggtct gcttgcgatg
     3481 acctttgaca agttcctagt atcttaattt tgataacaat ttctctggtt tgcctgcaaa
     3541 ttcttcgagc agaatttctt tatttgtgag tgcgagcaag gtttcagtga gtactttatt
     3601 gacaggagtt ggaattccac gtgtcttccc ttcgcggacg acggctccat gtaaaaattc
     3661 cacttcgctt tgcgggcgac ccgaatacag gtcaatatgg aaagatggca tcttaccgcc
     3721 gcgtccgctg cctgcggctt tggataggaa aggtttggag agccaaagcg gaagtttggt
     3781 tgcaaatgcc aaagctcgaa cgggtgttcc gggcaaatca gtcacttcga ggttcatgga
     3841 tgccatcact gccaaacatt ccttgagcat atcgatctca atcttgtata ggcgcttatc
     3901 ggcaaagact tctgctgccg tcatgttgag aatggcagat gtgggattcg ccaccaaatt
     3961 ggtcagcatc ttcgaccatt tcatgcttaa tgcatcagga tacaagcggc acttaagata
     4021 cgcattatca aacgcagcaa caagcttttc tgagaggggg tgtcccgctg cgatgcccac
     4081 gccacgcaat ttctcaagga caatgctgcc aggtccacgc ctgccaatgg cagttgtgac
     4141 cgttccgtaa ataactttat cgggtccgag agtacgggca atgataggct cgttatccac
     4201 accattggag agacaaagca aaggcgggag tttatctgcg aagggcttca agccttccat
     4261 ggcagaaacg gtatcaaatg atttcaatgc aaagagcgcc gcatcgaaag gaccgtattt
     4321 caaagcatct tccaacgaag gctcgatgat gaaagacttt gaatcaataa tacttacatc
     4381 tttcgtattc cgcctttcgt ccacggttag gtccagccgc agacctttgg cttttaattc
     4441 ttccaccatc tttggctttt ctacga
//
LOCUS       DAOQNJ010000169         4465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12160_length_4465_cov_9.504535, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000169 DAOQNJ010000000
VERSION     DAOQNJ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12160_length_4465_cov_9.504535"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PLG52_06220"
     CDS             <1..1000
                     /locus_tag="PLG52_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB41075.1"
                     /translation="AAKRAVDKFGTGTNGVRTLAGTLTIHNELEETISNFKHAEAAIT
                     YTSGYVTNLTVISTLMGRGDYVFSDKINHASIVDGCLMSGAEFRRFRHNDMEHLEGLL
                     KNAPADVSKLVIADSVFSMDGDIIDLPKIVELSKKYNAWLMIDEAHSVGVLGKTGTGI
                     EEHFNMYGSIDIKMGTLSKTIPSVGGYVAGKKELIDYLRHGSRAYIFSAALPPAQAAA
                     ANEAFKVILDEPERLDRLNANTQQFIGGLKSMGFDTLLTETAIVPVLCGTDEKAMELT
                     KRCQEQDVFVLPVVSPAVQEGMSRLRATVTAAHEPADIEHAMDVIYQSGKAMGMVK"
     gene            1205..2302
                     /locus_tag="PLG52_06225"
     CDS             1205..2302
                     /locus_tag="PLG52_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNB41076.1"
                     /translation="MKWQWKFGTFAGIDVYIHATFLLLIGWVGYSHWLEKNSWFDVLS
                     GIGFILALFLCVLLHEYGHALTARKYGIKTRDITLYPIGGVARLERMPEKPIEELWVA
                     LMGPAVNVVIAAILFAVLLLTGNLVPLTELGVASGSFLERVMVVNVSLVLFNLIPAFP
                     MDGGRVLRAILAMRMDYVKATQIAASIGQGLALLFGLIGLFGNASLLFIAFFIWIGAS
                     QEASAVQMKNVISGIPVGRAMLTDYKSLSPRDPLSRMAQLVLAGSQHDFPVIDDAERI
                     VGVVTRDDFLAALTQHGQNIAVSAVMRNNPPEVDSYDMVEVALTRIQESGFPTLPVTH
                     SGQLVGIITAENITEYLMIRTALRTAQVVSI"
     gene            2587..>4465
                     /locus_tag="PLG52_06230"
     CDS             2587..>4465
                     /locus_tag="PLG52_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNB41077.1"
                     /translation="MNWHTIETNAALNELASSSETGLTSAQVEESKKKHGANELIERG
                     GRTPWQILWEQITATMVLILIAAAVVAGLLGDTKNTIAILSIVALYALLGFIQEYRAE
                     QAIAALKKMSVPNVRVIRDGKMQELSARELVPGDIIQLETGNVIPADLRLVEAVNLRI
                     QEAALTGESEAIEKHVAALSNEDLPLGDRRNMAYMGTVITQGRGLALVTATGMNTELG
                     KIADMIQAVKQEQTPLQRRLDALGKTLALIGVGIAVLIFILGIIRGDEVRHMLLTAVS
                     VAVAIVPEGLPAVVTITLALGAQRMLQRQALIRKLPAVETLGSVTVICSDKTGTLTEN
                     RMTVVVLDVAEHALDLTGDVARDGSLHATRGLGAPAQSSLSLAAIGGALCNDAKLVDE
                     GDDRFHTMGDPTEGALVVAAAKMGYWKSSLDSSFPRAAELPFDSDRKRMTTVHHLGQY
                     DPTVLSGLEIGDTRYIAFTKGGVDGLLDIASHVWADGKSVPLDDAWRSKIEASNERLA
                     RKGMRVLGVGFRLINAIPEIIQTDLEQNITLVGLFGMIDPPRNEVKDAVAIAKTAGIR
                     PIMITGDHPLTAMEIARQLGITDNGRVLTGVELEKITMDELKTMLDDVNVFARVAPEH
                     KL"
BASE COUNT         1053 a         1217 c         1141 g         1054 t
ORIGIN      
        1 agccgccaaa cgcgccgtgg acaaattcgg caccggcacc aacggcgtgc gcaccttggc
       61 tggaaccctc actattcaca acgaactcga agaaaccatt tcaaacttca agcatgcaga
      121 agccgccatt acctatactt ctgggtatgt caccaacctg accgtcatct ctaccttgat
      181 gggacgcggc gactacgtct tctcagacaa gatcaaccac gcctccatcg tggacggttg
      241 tttgatgtct ggtgcggaat tccgccgttt ccgccacaac gacatggaac atctcgaagg
      301 cttgctcaag aacgctcctg ccgatgtctc caagctcgtc atcgcggact cggtcttcag
      361 catggacggc gatatcatcg acctgcccaa gatcgtggag cttagcaaaa aatacaacgc
      421 ctggctcatg atcgacgaag cgcactcggt gggcgtgctc ggcaagaccg gcaccggtat
      481 tgaagaacac ttcaacatgt acggctccat cgacatcaag atgggtaccc tcagcaagac
      541 catcccttcg gttggcggat atgtggctgg caaaaaagaa ctgatcgatt acctgcgcca
      601 cggcagccgt gcgtacatct tctcggcagc gctcccacct gctcaggcag ccgccgcgaa
      661 cgaagccttc aaggtcattc tcgatgaacc cgaacgcctc gaccgcttga acgccaacac
      721 tcaacaattt attggtggct tgaagagcat gggctttgat accttgctca ccgaaactgc
      781 catcgtccct gtgctgtgcg gcaccgatga aaaagccatg gaattgacca aacgctgcca
      841 agagcaggat gtcttcgttc tgcccgtcgt ctcgcctgca gtgcaagagg gcatgtctcg
      901 cttgcgtgcc acggtcactg cggcacacga acctgcagat attgaacacg ccatggatgt
      961 gatctaccaa tctggcaaag ccatgggaat ggtcaagtaa ttagatagtt agataaagtt
     1021 gaaaatcgcc tgtcctatcg acaggcgatt tttttatcgg cgttcgctgc tgcttggaga
     1081 gacagaccaa gcccaagcgg agtgggaagc ggggttttca tcctgatagt tcttgataga
     1141 atttttataa catttttcta ataaattcct tgtacaatgc cgtcaacgtt acaaggagca
     1201 aactatgaaa tggcaatgga aatttggaac atttgcagga attgatgtct acatccatgc
     1261 caccttttta cttttgatcg ggtgggttgg gtatagtcat tggttggaaa aaaatagctg
     1321 gttcgatgtg ttgagcggca ttggctttat tctcgcgctc tttttatgcg tactcttgca
     1381 tgaatatggg cacgcgctga ccgctcgtaa atacggaatc aagacgcgcg atatcacact
     1441 ctacccgatt ggtggcgtgg cgcgtttgga acgcatgcct gagaaaccga tcgaggaatt
     1501 gtgggtggcg ttgatgggac ccgccgtgaa cgtggtgatt gccgccattc tttttgcggt
     1561 tcttttactg accggaaacc tggtgccgct caccgaactt ggggtggcat ctggaagttt
     1621 tctcgagcgc gtgatggtgg tgaacgtttc actggtattg ttcaacctca tccccgcctt
     1681 ccccatggac ggtggacggg tgttgcgagc catcctcgcc atgcgcatgg actatgtgaa
     1741 agcaacacag atcgccgcaa gtattggtca gggactggcg ttgttgttcg ggttgatcgg
     1801 tttgttcgga aatgcgagct tgttattcat cgccttcttt atttggatcg gtgcttcaca
     1861 agaagccagc gccgtgcaga tgaaaaatgt catcagcggc attccagtgg gacgcgccat
     1921 gttgaccgat tacaaaagcc tctcgccacg tgacccactc tcgcgcatgg cacagttggt
     1981 tctggcaggt tcacagcacg acttccccgt gatcgacgac gcagaacgca tcgtcggcgt
     2041 agtgacccgc gacgatttcc tcgccgcact cacccaacac ggacaaaaca tcgccgtctc
     2101 agcagtgatg cgtaacaatc cgcctgaagt ggattcatac gatatggtgg aagttgccct
     2161 gacgcgtatt caagagtcgg gcttccccac gttgccggtt actcattccg gtcagctggt
     2221 tggcattatc actgccgaga atatcaccga atatttgatg atccgcacgg cattgcgaac
     2281 cgcccaagtg gtttcgattt aatgagggtc tctgcaagtg cagatgagag atttcttaac
     2341 cacgaaaatc gttttttcca tatactaggc acaactccga tggggagtag ccgcccacat
     2401 gttgggtagc tcagccgtca atacgtggta cattgtgcca ccggctgttc tgtaaaggca
     2461 agaccatcgt caacatcctt gacgatggtc tttttatttg cggaaagtat tacgccttcc
     2521 tcaaacggca cgtcaggtta aatacatcaa aatagattaa acttattagg aacacggaaa
     2581 caatacatga actggcatac aatcgaaacc aatgcagctt tgaacgaact tgcttcctct
     2641 tccgaaacgg gtctgacttc cgcacaggtt gaggaaagca aaaagaaaca cggcgcgaac
     2701 gagctcatcg aacgcggcgg acgcacccca tggcaaattc tgtgggaaca gatcaccgcc
     2761 accatggtgt tgattctcat tgcagccgcc gttgtggcag ggttgttggg cgatacaaag
     2821 aacaccatcg ccattctttc cattgtggcg ttgtacgcct tgctcggctt tattcaagaa
     2881 tatcgcgccg aacaagccat tgccgcactc aagaaaatgt cggtacccaa tgtgcgtgtg
     2941 atacgcgacg gcaaaatgca ggaactctct gctcgcgagc ttgtgcccgg cgatatcatt
     3001 caactcgaaa ctggcaacgt catccctgca gatttgcgtt tggttgaagc cgtcaacttg
     3061 agaattcaag aagccgcgct cactggcgaa tcagaagcca ttgaaaaaca cgtggctgca
     3121 ctttcaaatg aagaccttcc gcttggcgac cgtcgcaaca tggcatacat gggcacggtc
     3181 atcacccaag ggcgcggact tgccctcgtc actgccaccg gcatgaacac cgagcttggc
     3241 aagatcgcag atatgatcca agcggtcaaa caagaacaga ctccgcttca acgtcgcctt
     3301 gatgcgcttg gtaaaaccct tgcgctcatt ggcgtgggca ttgcggtgct cattttcatt
     3361 ctcggtatta ttcgtggcga cgaagttcgt cacatgttac tcacggcggt cagtgttgca
     3421 gttgccattg tgccagaagg cttacctgcc gttgtcacca tcacactcgc gctcggtgcc
     3481 caacgtatgt tgcagcgtca ggcgctcatt cgcaaactcc ccgctgtcga aaccctcggc
     3541 tcggtcactg tcatctgctc cgataagacc ggtacactca ctgaaaaccg gatgacggtc
     3601 gttgtgttgg atgtggcgga acatgccctc gacctgaccg gtgacgttgc ccgcgacggc
     3661 tcgcttcatg cgacccgtgg gctgggtgca cctgcccaat cctctctctc actggcagcg
     3721 attggtggcg cgttgtgcaa cgatgccaaa ttagtggacg aaggcgatga ccgcttccac
     3781 accatgggcg accctacaga aggtgcactc gtggttgccg ccgcaaagat ggggtattgg
     3841 aaatcttccc tcgattcatc tttcccccgc gccgccgaat tacccttcga ctctgaccgc
     3901 aaacgtatga ccaccgtgca tcaccttggt caatacgacc cgaccgtgct ttctggttta
     3961 gagatcggcg atacacgtta tatcgctttc accaaaggcg gtgtggacgg tctgctcgac
     4021 atcgcaagcc acgtctgggc agacggcaag tctgttccgc tcgatgatgc ctggaggtcc
     4081 aagattgaag cgtcgaacga acgccttgcc agaaaaggta tgcgcgtcct cggcgttggc
     4141 ttccgcctta tcaatgccat ccctgagatc attcaaactg accttgaaca gaatatcacc
     4201 ttggtaggtt tgttcggcat gatcgaccca ccacgtaacg aggtcaaaga tgcggttgcc
     4261 atcgccaaga cggctggcat tcgccctatt atgattactg gtgaccatcc actgaccgcg
     4321 atggaaatcg cacgtcaatt gggcatcacc gataacggac gtgtgctgac cggcgttgag
     4381 cttgaaaaaa tcacgatgga tgaattaaag accatgctcg acgatgtgaa cgtctttgca
     4441 cgtgtagcac ccgagcataa actcc
//
LOCUS       DAOQNJ010000170         4456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12191_length_4456_cov_6.335378, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000170 DAOQNJ010000000
VERSION     DAOQNJ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12191_length_4456_cov_6.335378"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLG52_06235"
     CDS             <1..323
                     /locus_tag="PLG52_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41078.1"
                     /translation="QQDQNWAEVRAACHRLAVETWQRIHDLTGETPLHSDPEPWFAQM
                     AVATLPADSDLVALKSRLYDEHRIEIPLIAWNEKKLIRLSVQGYNSRRDMDKLLSALN
                     TLLR"
     gene            332..1348
                     /locus_tag="PLG52_06240"
     CDS             332..1348
                     /locus_tag="PLG52_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNB41079.1"
                     /translation="MRKRISFLFALIILLVLSITPKQTVEAKPFANITSPSQLIDAVN
                     SLRVANGLSPLPVHQALMQSAQSQADYMAATGNVTHSRPSGTYTQQLLALGFPLAGDL
                     SAGGFRSENILSTYGPLVWNGVPSAWQDAQHMNTMLSGNFTHIGAGVSQSGDMYYYTV
                     DTAAATSSGQQQDSAATVLTSVPGGSSDAGVSQFMVPVTLNTPRPDGNVYHKVQYGQS
                     LWSIAIAYGTTIKNIQALNNLGEASTVYQGQELLVVTGATQAAAPTLDATATTQATAT
                     LAPTSTNVPTSTPELALLTPTEEAPVEANTKPASSRVLIVVLIVAAFVGAGMAVWLIR
                     DPGN"
     gene            1451..2329
                     /locus_tag="PLG52_06245"
     CDS             1451..2329
                     /locus_tag="PLG52_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB41080.1"
                     /translation="MEITLALGGGGAKGNAHIGVLRRLEKEGYKIRSVAGTSFGGMVA
                     ALYAIGYSPREIQAIFESVDQESLYGRASQDGPSLLGMAGVRQLLERVIGEKTFKDTR
                     IPCAVTAVDINTGGEVVLNEGSIKNAVLATIALPGIFPVQKINDWELVDGGVLNPVPV
                     SVARLLSPDLPVVAVVLNDPIDKPVRGYNLPLPSIVPRTIAERLARISLAQSLDIFLR
                     SVDVSSRYVSHLRLQVDKPAIIVRPDVHHIELLDQVVVEDVAALGEQALEQVLPDLKR
                     KTTWAARLGRRVFGAN"
     gene            2332..2571
                     /locus_tag="PLG52_06250"
     CDS             2332..2571
                     /locus_tag="PLG52_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41081.1"
                     /translation="MTRDLRKYMRDTNTRIVVGAILMLFIIGLGLIWLIYGFGAAVTG
                     FLCIAAAFIPIGLVFLFLFGMDWFVKRANPDERND"
     gene            2543..3220
                     /locus_tag="PLG52_06255"
     CDS             2543..3220
                     /locus_tag="PLG52_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41082.1"
                     /translation="MQTQTNETIEFNGWTMRYRSAETSHPRLLVMIHGLTGDENSMWV
                     FARRFSPNYWIIAPRAPHPANPQGFSWRANPLSIDEARANQTNRPRLDALKEAAEGLI
                     RLIDEYAASVKVEAGIFDAIGFSQGAAMVNVLGMLYPNRVRKMGILAGFAPHGLDEYA
                     SQKVLAGKQVFVAHGTQDDMVPIERARESMKLLEQAGASITYCEDEVGHKLSSNCLRA
                     LEQYLTD"
     gene            3304..>4456
                     /locus_tag="PLG52_06260"
     CDS             3304..>4456
                     /locus_tag="PLG52_06260"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNB41083.1"
                     /translation="MRKYLSRRDFIKLAGLGVGAMAFTRLSPSDVDYRWEIFSQPKRL
                     PQFPGSAIIGRVCEPDIDLRSSPTNNPNLNNSLSKLNADTLVEWNREVIGNVIGGLPN
                     QRYVETPQGYVYASVVQPTKNIPNTPVTELPNGQGFWAEVTVPYVELAHEGTVASPWL
                     QDHITYNFPPRLYYGQVVWIDQIRTTNGYPEYRWNEDAAGRGYGYGGGYGEFYWADGA
                     GLKILTDADISTINPEVDPNEKTISIDLDYQTLSCYEGAREVYFCRISSGKKYDPVTG
                     QVTDAYATPAGSLLTYWKIISKNMTAGNEGSGYSTPAVPWCTFIASGGVAIHGAFWHN
                     AFGERRSHGCVNVTPEDSKWIFRWTMPYVSLAAGEERRELPDHGTTVTST"
BASE COUNT         1043 a         1046 c         1186 g         1181 t
ORIGIN      
        1 ttcaacagga ccaaaactgg gcggaggtaa gagccgcgtg tcatcgcctg gcagtagaga
       61 cgtggcaacg catccatgac ttgacgggag aaaccccgct tcattccgac cctgaacctt
      121 ggttcgccca aatggcagtt gccaccctgc cagcggactc tgacctcgtt gccctcaaat
      181 cgcgcttgta tgatgagcat cgtattgaaa ttccgctcat cgcgtggaat gagaagaaat
      241 taatccgttt gtctgtgcag ggatataatt ctcgtcgaga tatggacaaa ttattatctg
      301 cattgaatac gttgttaaga tgaaagcata tatgagaaaa cggatttcgt ttttattcgc
      361 cttgattatt ctgctcgtat tgagcattac cccaaaacaa actgttgaag caaaaccatt
      421 tgcaaatatt acatccccat cccaattgat cgatgcggtc aatagtttgc gggtcgcaaa
      481 tgggctttca cccttgcctg tccatcaggc attgatgcaa tctgctcaat cgcaagcaga
      541 ttacatggca gctacaggca atgtaacaca ctctcgcccc agcggcacct acacccagca
      601 attgcttgcg ctcggctttc cgttagccgg cgatctaagt gcgggcggct ttcgctccga
      661 aaatatctta agtacctacg gacctttagt atggaatggt gttccgagcg cctggcaaga
      721 tgctcaacat atgaacacta tgctttcggg caactttaca cacattggcg ctggcgtttc
      781 acaatcaggc gatatgtatt attacactgt ggataccgcc gctgcaacca gctcgggtca
      841 acaacaagat agtgctgcaa cggttctcac cagcgtgccg ggcgggtcaa gtgatgcagg
      901 tgtcagtcag ttcatggtgc cggtcacatt gaatacgcct cgtcccgacg gaaatgtgta
      961 tcataaggtg cagtatgggc aatcgttgtg gagcattgcc attgcctatg gcacaaccat
     1021 caagaacatt caagcgctaa acaaccttgg cgaagcctca accgtatatc aagggcaaga
     1081 gctgctcgtg gttacgggcg ccactcaagc agcggctcca actttggatg ccacagctac
     1141 gacacaagcc acagcaacct tggctccaac ttcaaccaac gttcctacct caaccccaga
     1201 actggcactg cttaccccta cagaagaagc acccgttgaa gcaaatacaa agccagcctc
     1261 ttcaagagtg ttgattgtcg ttttgattgt ggcggcattt gtaggggcgg gaatggcagt
     1321 ttggctgatt cgcgacccgg ggaattaagt ttctcagaaa atgtgttgaa actatgttgt
     1381 ttttttgtaa gggtataatc cctcgcgttt gatttgtccg ttaattgaat attgaaaggc
     1441 atcgaagaaa atggaaatta cgttggcatt aggcggtggg ggcgccaagg gcaatgctca
     1501 cattggcgtg ttacgtcgtc ttgaaaaaga aggatataaa attcgctctg tcgcagggac
     1561 tagttttggt ggaatggtcg ctgctttata tgctattggc tactctccgc gagaaattca
     1621 agcaatcttt gaatcagtag accaggaaag cctttatggg cgtgcctcgc aagatggtcc
     1681 gtctctattg ggaatggctg gggttcgtca actgttagaa cgtgtgatcg gtgagaaaac
     1741 cttcaaagac actcgcattc cttgtgccgt cactgccgtg gatatcaaca cgggcggtga
     1801 ggtggtactt aatgaaggca gtatcaaaaa tgctgtactc gccacaatcg ctttgccggg
     1861 tatcttcccg gtgcagaaga tcaacgattg ggaactagtg gatggcggcg tcttgaaccc
     1921 ggtgccggtg tctgtggctc ggctcttatc gccagacctg ccggtggttg ccgttgtgtt
     1981 gaacgaccct attgataaac ccgtgcgcgg atacaacttg ccgctgccca gcattgttcc
     2041 acgtacgatt gctgaacggc tcgcaaggat ttcgttagcc cagtccttgg atatcttctt
     2101 gcgctctgta gatgtttcca gccgctatgt ttcgcatttg cgcttgcaag tggataagcc
     2161 cgcgatcatc gtccgcccgg atgtgcacca tattgaattg ttagaccagg ttgttgtgga
     2221 agatgtggca gccttaggcg agcaggcatt agagcaagta ttgccagacc tgaagcgcaa
     2281 aaccacatgg gctgcccgtt tgggtcggcg tgtgtttgga gcaaattaac aatgactcgt
     2341 gacctgcgca agtacatgcg tgacaccaat acgcggatcg tcgtcggcgc gattctgatg
     2401 ttgtttatca tcgggctggg gctgatctgg ttgatctacg gctttggcgc agcagtgaca
     2461 ggttttctct gtattgccgc ggcattcatc ccgattggtt tggtcttttt atttttattt
     2521 ggaatggatt ggttcgtaaa acgtgcaaac ccagacgaac gaaacgattg aatttaacgg
     2581 ttggacgatg cgttatcgct cagccgaaac ctcgcatccg cgtttgttgg tgatgatcca
     2641 cggtttgacc ggcgacgaaa actccatgtg ggtttttgcg cgccgttttt ctccaaatta
     2701 ttggatcatc gctccacgtg cgccacaccc tgctaacccg cagggttttt catggcgtgc
     2761 aaatccgctt agcattgacg aagcgcgtgc gaatcaaaca aaccgcccaa ggttggatgc
     2821 gctgaaggaa gcggctgaag ggttgataag gcttattgat gagtatgcag cttcggttaa
     2881 ggtggaggcg ggcatcttcg atgcgattgg gtttagtcag ggtgcggcaa tggtcaatgt
     2941 gttggggatg ttgtatccga accgtgttcg aaaaatgggc attttggctg ggtttgcccc
     3001 tcatggcttg gacgaatatg cgagccaaaa ggttttggcg ggtaagcagg tttttgtggc
     3061 gcatggcaca caagacgata tggtgccgat tgagcgggcg cgtgagtcga tgaaattgct
     3121 ggagcaagcg ggggcttcga tcacatattg tgaagatgaa gtagggcata aattgagttc
     3181 gaattgtttg cgggcactgg agcagtacct tacagattaa tctgtttctc accacgctgt
     3241 tgacgctgtt tttatttgca ggtatgcttg ttgacgtaat ctcttctata taaaggtgaa
     3301 actatgagga aatatttatc gcgacgcgat tttataaagt tggctggttt gggcgtgggt
     3361 gccatggcat ttacgcgtct atctccgagt gatgttgatt atcggtggga gatctttagc
     3421 cagcccaagc gtttaccaca gttcccgggg agcgctatca ttggacgtgt gtgtgaaccc
     3481 gatattgatt tgcgcagcag cccaaccaat aacccgaatt tgaataattc actaagcaag
     3541 ttgaatgccg acactctcgt agagtggaat cgcgaagtga taggcaatgt catcggtggg
     3601 ttgccaaacc aacgctatgt ggaaacgccg cagggctatg tctatgcctc tgttgttcag
     3661 ccgaccaaga acattcctaa tacgccggtc accgaactcc cgaacggaca aggcttctgg
     3721 gcagaagtaa ctgttcctta tgttgagttg gctcacgaag ggactgtcgc ttcgccttgg
     3781 ttgcaagacc atattacgta taacttcccg ccgcgtttgt attatgggca ggtggtctgg
     3841 attgaccaga ttcgtactac taatggttac cctgaatatc gctggaatga agatgctgct
     3901 ggtcgtggct atggctacgg tggtgggtac ggtgagtttt attgggcaga tggtgctggg
     3961 ttgaagattc tcaccgatgc tgatatttcc accattaacc ccgaagtgga cccgaacgaa
     4021 aagaccatct ccatcgactt ggactatcaa actctttcct gttatgaagg tgcacgtgag
     4081 gtgtacttct gccgcatttc ttctggcaag aaatatgacc ccgtcacagg gcaggtcact
     4141 gatgcatatg ccaccccggc tggttccttg ctgacctatt ggaaaattat ctccaagaac
     4201 atgacggcag gtaatgaagg ttctggctat tccaccccgg cagtgccttg gtgtacgttt
     4261 attgcctctg gcggcgtggc aattcatggt gccttctggc ataacgcctt tggtgaacgt
     4321 cgctcgcatg gctgtgtcaa tgtaacgcct gaagattcca agtggatttt ccgttggacg
     4381 atgccatacg tttctcttgc cgctggcgaa gaacgccgcg aactgccaga ccacggcacc
     4441 acagtcactt ctaccg
//
LOCUS       DAOQNJ010000171         4455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12198_length_4455_cov_7.922045, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000171 DAOQNJ010000000
VERSION     DAOQNJ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12198_length_4455_cov_7.922045"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLG52_06265"
     CDS             complement(<1..430)
                     /locus_tag="PLG52_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41084.1"
                     /translation="MSQPNLPDFDSLWDYSNPDQTETKFREVLPQFPDGNERLELLTQ
                     IARTQGLQRKFDEAHKTLDEVEGQLSVDSSRARVRYLLERGRVFNSSKHPEEARPFFE
                     QALTMAQELKEDFYAVDAAHMLAIVADPASSLDWNLRAIQM"
     gene            complement(534..986)
                     /locus_tag="PLG52_06270"
     CDS             complement(534..986)
                     /locus_tag="PLG52_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB41085.1"
                     /translation="MISYSIRKATPEDLPVLVELCAEHAEYEQAEYSPLGKADRLREH
                     FFGTPPSAFCLVAEADKTLAGYATYSKEFSTWDAGFYIHMDCLYLRPSFRGYGIGKEL
                     ITQIREEARGLGCNLIQWQTPKFNTRAIKFYERIGAKQKEKVRFYLNV"
     gene            complement(983..1864)
                     /locus_tag="PLG52_06275"
     CDS             complement(983..1864)
                     /locus_tag="PLG52_06275"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNB41086.1"
                     /translation="MELHHLKTFVIVAEERNLTRAADRLFMTPPSVSAHIKALEDELG
                     VTLFVRKPQGMEITEHGEILRVKAEKILNSTREMVNLSKKLQASLTGTTRIGLSATSV
                     YQRAAKLISQMQKEHPDIELTFNASCTHKIIQGLKNHSMDAGYMFGAIEDEAITGYEL
                     DVADLVITAPAAWKDQVAEAGWKEIAALPWIGSSYYCPFQTAAEKLFKKRKLAMNHVV
                     WMDDEFNKCALVEAGVGLALLERSEAEQAALGGKIFIWKTEPIKCKLSWVYLRNRGDD
                     PLIAALTAEVLNIWGKA"
     gene            1992..2411
                     /locus_tag="PLG52_06280"
     CDS             1992..2411
                     /locus_tag="PLG52_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB41087.1"
                     /translation="MVTLKPKARVTATPAADPAIANAFFSAKLTYETDPSDVYADMKN
                     ELADFILIDVRSTADYAKSHAQGAISLPQVNINTETTSKLPKEKLLVVYCWGPGCNGA
                     TKAAAKLSALGFHVKEMIGGIEYWEDKERYPVERGWL"
     assembly_gap    2419..2518
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2730..3605
                     /locus_tag="PLG52_06285"
     CDS             2730..3605
                     /locus_tag="PLG52_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB41088.1"
                     /translation="MKTKHWFIFITLGLIWSSSFLWVELALREVGPITLVAFRAFFGF
                     LFGVAVVVTQRIPLPRTLKEWIPLIVLGLTNIAIPFFLISWGQKSIDSGVASILDATV
                     PLFTILVAHFMLHDDKMTTPKVLGLLTGFAGVVVLMSKDLGASTSSLLGQLAVILACI
                     FYAASSIIARVFTEGTSATMRGLGPLVSASAVMITSAFIFESPIKIPTLPITWIALLW
                     MGILGSGLAFIMLFYLIHEIGPTRTTMVTYLFPLGGVTLGVIFLKEALTWQIVAGAVL
                     IILSLVIANWQTKQA"
     gene            3717..>4455
                     /locus_tag="PLG52_06290"
     CDS             3717..>4455
                     /locus_tag="PLG52_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB41089.1"
                     /translation="MKTKHWIAFIALGGIWSSSFLWIKIGVQEASPNVLVAFRMLFGA
                     LMSTAIALYQRSEWPTDRKTLINFAILGPTSLAIPIFFISWGEQVIDSAVASILNATV
                     PLFTLILAHFWLQDDKITPQKLIGLLVGFAGILILFSKDLLASQHQNVIGQLAVIVAA
                     LFYAWSAVFGRKTTAHIKGMSRAALPVITATIFMWLTIPFTEKPFVLPQLPITWISFL
                     WLGLLGSGITSLLFYYLLHEIGPTRTTL"
BASE COUNT         1025 a         1165 c         1026 g         1139 t
ORIGIN      
        1 ccatttgaat ggcacgcagg ttccagtcga gggaagaggc ggggtctgcg acgatcgcca
       61 gcatgtgagc cgcatccacc gcataaaaat cctctttcaa ttcctgcgcc atggtcaatg
      121 cctgctcaaa gaacggacgg gcttcttcgg gatgtttgga tgaattgaaa acgcgcccgc
      181 gttccaaaag ataacgaact ctggcacgcg aagaatcgac agagagttga ccttctactt
      241 catccaaagt tttatgtgct tcgtcaaact ttctttgcaa gccttgcgta cgcgcgatct
      301 gagtcaataa ctctaaacgt tcattgccat cggggaattg tggcaaaact tcgcgaaact
      361 tggtttcggt ttgatcgggg ttcgagtaat cccaaagcga atcaaaatca ggtaagttcg
      421 gttgtgacat ggtacatctc cataatacga taaagataat ctttggattt taagcgtaaa
      481 tggatggtag tccaagcccc atgggcaagc aacctaccgg agaagggtta tctctaaacg
      541 tttagataga atcgaacttt ttctttctgc tttgccccga tgcgttcgta aaattttatg
      601 gcacgtgtat tgaatttcgg cgtttgccat tggatgagat tacagccaag cccgcgcgct
      661 tcttctcgaa tctgagtgat cagttctttt ccaattccat acccgcgaaa agacgggcgc
      721 aggtaaagac aatccatatg gatataaaag ccagcatccc atgtggaaaa ttctttggaa
      781 taagttgcat agcctgccag agttttatcc gcttctgcaa caagacagaa ggcagaggga
      841 ggtgttccaa aaaagtgttc gcgcaaacgg tctgcttttc cgagcggaga atattccgct
      901 tgttcatact cagcgtgttc ggcgcacaac tcaacaagaa caggcagatc ttctggcgtt
      961 gcttttcgga tcgaatacga gatcatgctt tgccccaaat attcagaacc tcggcggtca
     1021 aggcagcgat gagaggatca tccccccggt tgcgcaaata gacccacgag agtttgcatt
     1081 tgatcggctc ggtcttccag ataaaaattt tcccgcccag tgcggcttgc tccgcttcgc
     1141 ttctttcgag cagcgccagc ccaacgcccg cctcgaccaa agcgcacttg ttgaattcat
     1201 catccatcca gaccacatga ttcatggcga gcttgcgctt cttgaagagt ttctcggcag
     1261 ccgtttgaaa gggacaataa tacgatgacc cgatccaagg cagcgccgcg atctccttcc
     1321 agcctgcttc agcgacttga tccttccacg ccgctggagc cgtgatgaca aggtcggcaa
     1381 cgtccaactc atagcctgtg atcgcttcgt cttcaatggc accgaacatg tacccggcgt
     1441 ccatcgagtg attcttcaat ccctgaatga tcttgtgcgt gcaagatgcg ttaaaggtca
     1501 actcgatatc cgggtgttcc ttctgcatct ggctgatcag cttcgccgca cgttgataaa
     1561 ccgacgtggc acttaaccca atgcgggtcg tgcctgtgag agatgcctgc aattttttgg
     1621 ataagttgac catctctcgc gtggagttca agatcttctc ggctttgacg cgcaggattt
     1681 cgccatgctc ggtaatttcc atgccctgcg gcttgcggac gaagagggtg acacccaatt
     1741 catcttccaa cgccttgatg tgcgcgctga ccgaaggcgg agtcataaac aaacggtcgg
     1801 cagcacgggt caggtttctt tcttcagcca caatgacaaa ggtctttaag tgatggagtt
     1861 ccatgcccgc agtatacatg attcccttct gccgagcgtt ttgcttcgcc gaacgctatg
     1921 ttcgaaagcc tcaaattcac aaaagggctt cttttccata cacttgaagc attccaactc
     1981 aaggagaaaa catggttacc ctcaaaccca aagcacgggt cacagccacc ccggcagcag
     2041 accccgccat tgccaacgct ttcttttccg ccaagctgac gtacgagacc gacccctcgg
     2101 atgtgtacgc agacatgaag aacgaactgg cagactttat cctgatcgat gttcgcagca
     2161 cagcagacta cgccaaaagc catgcgcagg gtgcaatcag tttgccgcag gtaaacatca
     2221 atacagagac aacgtccaag ctgcctaaag aaaagctgtt ggtggtctat tgctggggtc
     2281 ctggctgtaa cggtgcgaca aaagccgccg ccaagctcag tgctctcggc tttcacgtca
     2341 aagagatgat cggcggcatc gaatactggg aagataaaga acgctacccc gtggaacgcg
     2401 gctggctcta aaggagacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
     2521 ctgggaagat aaagaacgct accccgtgga acgcggctgg ctctaaagga gactcggcag
     2581 ccaaactgga aaagaaccct gcatccacac tcagggttta taaatcggat ggttgtcttc
     2641 tgccaacttg agatgcgcaa gaatcgggtg gtaaaaggta aagctgaccg cgtacaatcg
     2701 ctgcatacat taatcattta ggagttaaat tgaaaacaaa acactggttc atctttatca
     2761 ccctcggctt gatctggagt tcatcctttt tatgggtcga acttgctctg cgtgaggtcg
     2821 gtcccattac actcgttgcc ttccgcgctt tttttggttt cttgtttgga gtggcagttg
     2881 tagtgactca gcgcatccct ttaccgcgca ctctaaaaga atggattccg ctgatcgtgc
     2941 ttgggctgac caatatcgct attcccttct tcctcatttc ctgggggcaa aaatccattg
     3001 actcgggcgt ggcttccatt ctcgatgcca ccgtgccact ctttaccatt ctcgtggcac
     3061 acttcatgct gcacgacgac aagatgacaa cgcccaaagt gttgggctta ttgaccggtt
     3121 tcgctggtgt ggtcgttctg atgagcaaag accttggcgc ctccactagt tcattgcttg
     3181 ggcaattggc tgtcatcctg gcttgtatct tttatgcagc aagctccatc attgcacgtg
     3241 tgtttactga aggcacatcc gccactatgc gtggattagg tccgcttgta tctgcatcag
     3301 ccgtcatgat tacctccgct tttatcttcg aatcgccaat caagattccc accctgccca
     3361 ttacctggat cgccttgtta tggatgggta tcctcggttc gggactagcg ttcattatgt
     3421 tgttctatct cattcatgaa attggtccca cccgcaccac catggtcacc tatctatttc
     3481 cgctgggcgg agtcactctc ggcgtgatct tcctcaaaga agcgctgacc tggcagatcg
     3541 tggctggagc ggtgttaatc attctcagcc ttgtcattgc aaactggcaa accaaacagg
     3601 cataagaaca catacataga tgcatctggt ttcaacctct aattgtcact cattgttcct
     3661 agtcatttcg ccggttttgt gaagcccgca ccctcaattt cagaaagcag gatagtttga
     3721 aaacaaaaca ttggatcgcc ttcattgccc ttggcggcat atggagctct tccttccttt
     3781 ggatcaagat cggcgttcaa gaagccagcc ccaatgtgtt ggtggcattc cgcatgttat
     3841 tcggcgcgtt gatgtccact gccattgcgc tttatcaacg atcagagtgg cccacagacc
     3901 gcaagacgct catcaatttt gccatcctcg gacccaccag tttggcaatt cccatcttct
     3961 tcatttcatg gggcgagcaa gtcatcgact cggctgtcgc ttcgatatta aatgcaacag
     4021 tgcccttgtt cacactgatc ctggcacact tctggcttca agacgataag atcacgccgc
     4081 agaaactgat cggtttgctg gtaggcttcg caggcattct catcctgttc agcaaagacc
     4141 tgctcgccag tcagcatcaa aacgtgatcg gtcaattggc tgtcattgtt gcggcattgt
     4201 tctatgcctg gagcgctgtg ttcggtcgaa agaccactgc ccatatcaaa ggcatgtctc
     4261 gtgcggcgtt gccagttatt accgccacga tctttatgtg gctgacgatc cccttcaccg
     4321 aaaagccttt cgttctgccg caactcccca ttacatggat ctcattcctt tggctgggct
     4381 tactgggctc aggcatcact tcattgctct tttactatct cctgcatgag atcggtccca
     4441 cccgcaccac tttgg
//
LOCUS       DAOQNJ010000172         4428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12311_length_4428_cov_8.720101, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000172 DAOQNJ010000000
VERSION     DAOQNJ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12311_length_4428_cov_8.720101"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PLG52_06295"
     CDS             <1..793
                     /locus_tag="PLG52_06295"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNB41090.1"
                     /translation="QVKLGQFDEAIATIQTARNLHVRRPAGVWSSEDLLSYEIYIHLR
                     NNNMDKAEELLREGPTGTGHHFMQQMQAELLLKNGHFEEAEEILWNVIDTFPTLIMIE
                     PLQEVRILLSLALFWQNKVNQSVQVMTGALRLSAPEKFIRPFLDWGKECAPILLLVSE
                     TQSLNGEAQEFLSKLLKTLAITNEKTNPLKEDMEKLSTSASISIREQEILLLLSEGYS
                     NREISKKLSVSESTVKTHLGNIYMKLKVNSRMLAVSRAKQLKLIP"
     gene            1070..2392
                     /locus_tag="PLG52_06300"
     CDS             1070..2392
                     /locus_tag="PLG52_06300"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNB41091.1"
                     /translation="MNNYTFPHNFVWGAATASYQVEGAWNEDGKGESIWDRFSHTPDK
                     VTDGDTGDVACDHYHRYEDDIALMRKLGLKAYRFSVSWPRVIPNGRGKVNLKGLDFYD
                     RLVDTLCAANLEPFLTLYHWDLPQALQDQGGWENRDTAYAFADYAALMVKRLGDRVKS
                     WTTFNEPAVIAFVGNLYGAHAPGFKDEGIAYRVAHNVMVAHGLGVQAIRGANSNLKVG
                     IVLNLWPFETETDSPKAAAAAEALWKERETHFLEPIFKGHYSPEAYENIGHHLTTFKD
                     GDMALLSQELDFWGVNFYSRHVISAEGEFVKVPSSEYTEMDWEVCAPSLRRMLVRLYQ
                     DYKLPPLYITENGSAFKDEVSADGKIHDSRRLDYFKQHLIQTRLAMLDGADVRGYFAW
                     SLMDNFEWSFGYSKRFGLVHVDYKTQKRTIKDSGEWYAEVIRKNTVTE"
     gene            complement(2426..3412)
                     /locus_tag="PLG52_06305"
     CDS             complement(2426..3412)
                     /locus_tag="PLG52_06305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB41092.1"
                     /translation="MEYSRLGTTGMKVSRICLGCMGFGSAEGWSHSAWALDETGSREV
                     VKKALELGINFFDTANVYAYGVSEEILGRALKDFAKRDEVVIATKVFGKMNDKPNGGG
                     LSRKAILSEIDNSLKRLGTDYVDLYQIHRWDYETPIEETMEALNDVVRAGKARYIGAS
                     AMFAWQFQKALHVAEKHGWTRFVSMQNHLNLIYREEEREMLPLCRAEGIGSIPYSPLA
                     SGRLTRDWSAESTARSENDSIAKSKYDSTAETDQQVVKRVAEIAGKYGASRAHIALAW
                     LLQKAPVTAPIIGATKTRHLEEALGAFSVQLTPEDVAYLEEPYVPHRIVGHQ"
     gene            complement(3424..4386)
                     /locus_tag="PLG52_06310"
     CDS             complement(3424..4386)
                     /locus_tag="PLG52_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB41093.1"
                     /translation="MTTANLKGIGYLLLALLIGSLQPVAVKWIGGSYSVLEIVTFRSL
                     VALPATLIFFRLEGRRGLPTTIQPNLQLVRGIFLFLSYTTYMMGLAALHLADVEAIRF
                     SGPLMITILSVFMLNEKVDMRRWLALIVGFAGILLIVRPGSTHFNEGSLFVLISVLFY
                     ALTVILTRKMDKADSSATMAYFSSWVYLIAAFIFSPLAAMVGEIPNAHPSVAFLFHAW
                     TMPSILDLFIMSGLGIIWAIWMYFVTRAYSVAQASIIAPFEYASLPINVTWGFLLWHE
                     IPALLTIAGATLTLLSGLYILIQEQKKNKAPQVLVDNAALENEA"
BASE COUNT         1229 a         1033 c         1043 g         1123 t
ORIGIN      
        1 acaggtcaaa ttagggcaat ttgacgaggc aattgccacc atacaaactg cccgcaactt
       61 acatgttcgc cgcccagctg gtgtgtggag cagtgaagat ttactctctt acgaaattta
      121 cattcatctc cgtaacaaca acatggataa agccgaagaa cttcttcgcg aaggacccac
      181 tggaacagga caccatttta tgcaacaaat gcaagctgaa ctcttgttaa aaaatgggca
      241 tttcgaagaa gcagaagaaa ttctatggaa tgtgatcgat acattcccaa ctttgattat
      301 gatcgagcct ctgcaggagg tgcgcatctt gttatctttg gcattgttct ggcaaaacaa
      361 agtaaatcaa tcagttcaag taatgaccgg agctttacgc ctctcggcac ctgagaaatt
      421 tattcgtccc ttcttagatt ggggcaaaga atgcgcgcct attttattat tagtttcaga
      481 gacccaaagc ctaaatggcg aggcacaaga atttcttagt aaattgctca agacattggc
      541 aattacaaat gaaaaaacta accctctcaa agaggatatg gaaaagcttt ctacttcagc
      601 atccatatcc attcgcgaac aagaaatatt gcttttattg agtgaagggt attcaaaccg
      661 agaaatatcg aaaaagctat ctgtctctga aagtacggtc aagactcacc ttgggaacat
      721 ttacatgaaa cttaaagtca atagccgtat gttggcggta tcacgagcga aacaacttaa
      781 gcttatcccc taaacatggg ggtttgacat cctatctgaa tctctttata ctctgcaatc
      841 gatttcagaa agtactgaat tcatgcagac cttcaaagct atcttattta tctctcccct
      901 tttatatatt tctttcgccc tgtaatcaac agggcgattt ggattaatga cagaacggca
      961 acagagtaga ttagggctaa gtctgtaaag caggctaagg gtcaaaaaaa gaagcgtgag
     1021 tttaatcggc agtgtgtcca taagtatttt cacaaaagga tttgaatata tgaacaatta
     1081 cacttttcct cataactttg tttggggagc tgccactgcc tcttatcaag ttgaaggggc
     1141 gtggaatgaa gacggcaaag gcgaatcgat ctgggaccgc ttttcgcata ctccagacaa
     1201 agtgaccgat ggtgataccg gcgatgttgc ctgtgaccac tatcaccgct atgaagatga
     1261 tattgccctc atgcgcaagt tgggcttgaa ggcatatcgt ttttctgttt catggccgcg
     1321 tgtcattcca aatggtcgcg gaaaagtgaa tttgaaggga cttgattttt acgaccgctt
     1381 agtggataca ctttgcgcag cgaatctcga accgtttttg accctttatc attgggattt
     1441 gcctcaagcc ttgcaagatc aaggtggatg ggaaaatcgt gatactgctt atgcctttgc
     1501 tgactatgct gcgctgatgg tcaagcggct cggtgaccgc gtgaaatcat ggacgacctt
     1561 caacgagcca gcagtaatcg cgtttgttgg caatctttat ggcgctcatg caccgggttt
     1621 caaagatgaa ggcattgcct atcgtgtggc acataatgtg atggtggcgc atgggttagg
     1681 cgtgcaagcg attcgtggcg cgaattctaa tttgaaggtt ggcattgtgc tcaacttatg
     1741 gccttttgaa acagaaacgg attcgcccaa agctgctgcg gcagcagaag cactttggaa
     1801 agaaagggaa actcatttcc ttgaacccat tttcaaagga cattattcac ctgaagccta
     1861 tgaaaacata ggtcaccatc tcaccacatt caaagatggc gacatggcat tgctctctca
     1921 agagttggat ttttggggtg tcaattttta ttcgcgccat gttatcagcg ccgaaggcga
     1981 gtttgtgaaa gtgccaagtt ctgaatacac tgaaatggat tgggaagtat gcgctccgtc
     2041 tttgcgccgg atgttggtgc ggttatatca ggattacaaa ctgccgcctc tttacatcac
     2101 cgagaacgga tctgctttca aagacgaagt cagcgccgat ggaaaaatcc acgactcacg
     2161 ccgcctcgac tattttaaac agcacctcat ccaaactcgc ctcgccatgt tagacggggc
     2221 agatgtgcgc ggttactttg cctggtctct gatggataat tttgagtggt cgttcggcta
     2281 tagcaaacgc tttggtttgg tgcacgtgga ttacaaaact caaaagcgca cgatcaaaga
     2341 tagcggcgag tggtacgccg aagtgattcg taaaaacaca gtaacagaat aaaaaacaac
     2401 gcggatgggt tcatccgcgt tgtttttact gatgtccaac aatacgatgc ggcacatatg
     2461 gctcttcaag ataggcaaca tcctccggag tcaactggac tgaaaacgcg ccaagcgcct
     2521 cttccaaatg ccgggtcttg gttgcgccaa tgatcggagc cgtcactgga gctttttgca
     2581 acaaccacgc caaggcaata tgtgcccgcg atgcgccgta ttttccagca atttccgcta
     2641 cacgctttac aacttgctga tccgtttcgg cagttgagtc atatttactt tttgcgatcg
     2701 aatcattttc agaccgtgcg gtgctttcag cagaccagtc gcgagttaat cttccagagg
     2761 caagcggact gtatggaatg gaaccaatcc cctccgctcg gcagagcggc agcatttcgc
     2821 gttcttcctc tcgatatatg aggttgagat gattctgcat ggagacgaaa cgagtccaac
     2881 cgtgtttctc tgctacatgc aatgcctttt ggaattgcca ggcaaacatg gcagacgcgc
     2941 caatataacg ggctttaccc gcccgaacca catcgtttaa cgcttccatc gtttcttcga
     3001 tgggagtctc ataatcccag cggtggatct ggtacaagtc aacataatcc gtgccgagac
     3061 gtttgaggct gttatcgatc tcgctcaaaa tcgccttgcg cgacaatccc ccaccgttcg
     3121 gtttatcgtt cattttgccg aacactttgg tggcgatgac gacttcatca cgtttggcaa
     3181 aatctttcag agcgcgaccg agaatctctt cactcacacc ataagcatag acgttggcgg
     3241 tatcgaaaaa gttgataccc agttcaagcg cctttttcac gacctcgcgg ctgcctgttt
     3301 catcgagtgc ccatgcacta tgcgaccatc cttctgccga gccaaagccc atacagccaa
     3361 gacagatgcg cgaaactttc atgcctgtag tccccaaacg agagtattcc atgagattat
     3421 cctttacgct tcattttcca gggcggcatt atcaacaaga acttgaggtg ctttattttt
     3481 cttttgttct tgaatcaaaa tatacaagcc gctcaacaag gttaacgtgg cgccagcaat
     3541 ggtcaagagg gcgggaattt catgccagag caaaaatccc caggtcacat tgatcggcag
     3601 ggacgcatat tcaaagggtg caatgatcga ggcttgggca acactgtacg cgcgagttac
     3661 aaaatacatc caaattgccc agatgattcc cagccctgac atgatgaata ggtcaagaat
     3721 tgagggcatt gtccatgcgt gaaaaagaaa agcaacgctg gggtgtgcat ttggaatttc
     3781 gcccaccatt gccgccagtg gagaaaagat aaaagccgcg atcaaataca cccacgagct
     3841 gaagtacgcc atcgtggcgc tgctatcggc tttgtccatt tttcgggtga ggatcacggt
     3901 cagggcgtag aaaagaaccg agatcaaaac gaagagcgaa ccctcgttga aatgagtcga
     3961 accggggcga acgatcagca gaataccggc aaatccaacg atcaaagcca gccaacgccg
     4021 catgtccact ttttcgttca acatgaagac agacaaaatg gtgatcatca gcggacccga
     4081 aaagcggatc gcttccacgt ccgcgagatg caaggctgcc agccccatca tgtatgtagt
     4141 gtacgagagg aagagaaaaa ttccacgcac aagttgaagg ttgggctgga tggtagtggg
     4201 aagccctctt ctgccttcaa ggcgaaagaa gatgagcgtg gcaggcaatg ccaccaaact
     4261 tcgaaacgtg acgatctcca gcacagaata actaccgccg atccatttga ccgctacagg
     4321 ttgtaacgaa ccgatcaata atgccagcag aagatagccg atgcctttta aattagcagt
     4381 tgtcatatag tatgtttcta cgagatggaa ggttcagggg gcgatatg
//
LOCUS       DAOQNJ010000173         4414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12376_length_4414_cov_8.946777, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000173 DAOQNJ010000000
VERSION     DAOQNJ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12376_length_4414_cov_8.946777"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLG52_06315"
     CDS             <1..431
                     /locus_tag="PLG52_06315"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB41094.1"
                     /translation="TPQSGLSFTATVGVYSTTTTKNGCNDGYLVSESQPYDGKTMSAN
                     IDFDKTWDFINTGTCTWDEGYTFAFVPEFSTAPLYTVPKDYIIPKNGVFTKPGETRSF
                     KVSLRVPKDPGEYIWYYKIKDDGGQYFGSLVWVKVISVRE"
     gene            433..1059
                     /locus_tag="PLG52_06320"
     CDS             433..1059
                     /locus_tag="PLG52_06320"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB41095.1"
                     /translation="MKKIILPLIIVVLLSACAPATPIEPTPDVNAVRTSAASTVIAEF
                     TLTAAFFTPTPLPLPTETPTPFPTETPAVLPSIDPTLAALTPDALCDDYDFDPATIDV
                     TIPDGTPMTPGQDFVKTWKIKNTGICTWDTSYKAIFSYSNPPNQQMSAQPIPLAALVA
                     PGQEVDVSVQFTAPTTPGEYLGVWQMVNAKGIPFGQPGKQLTVKIVVQ"
     gene            1049..2095
                     /gene="queG"
                     /locus_tag="PLG52_06325"
     CDS             1049..2095
                     /gene="queG"
                     /locus_tag="PLG52_06325"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNB41096.1"
                     /translation="MFNNSITLKQAIKEKARQLGFFLAGVTSSASLAHFDIFQEWLDA
                     NHHASMDYLASDRSRLRRADPKQILPECKSVLVLALPYSPISKNSSEFQIASYALGDD
                     YHEVIPPRLQEIVQFIEQQVGHPIPNRYYTDTGPVLERELAQRAGLGWIGKNSMLIHP
                     QKGSTFLLAEILLGIELEPDEPFQTDHCGTCTRCMDACPTQCILPNRTIDSNRCISYL
                     TIENKVDIPEALRPRIQNWVFGCDICQQVCPWNRFSLLADPDLKPSIPLPVLTSDLLL
                     TSSEFNQRFKRSPIKRTKRRGYLRNLAVAVGNQKNKKDLPLLHQATQDEETLVQSHAQ
                     WAIETINRNNTGNL"
     gene            2092..2688
                     /gene="rdgB"
                     /locus_tag="PLG52_06330"
     CDS             2092..2688
                     /gene="rdgB"
                     /locus_tag="PLG52_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNB41097.1"
                     /translation="MSKLLIATNNQGKVRELQELLTGSGIEFVTPAQINLQIDVEEDG
                     STYQENAGKKAIAFAKASGLISLADDSGLEVEALGGAPGLYSARYSPKPGANDKDRRD
                     FLLSNLQDKPRPWKAHFHATIAVANPTGEVEFTDGNCYGEIIPEERGSGGFGYDPIFF
                     MPELGKTMSELDMSEKNRLSHRARAVIAAMPILKRIFA"
     gene            complement(2771..3538)
                     /locus_tag="PLG52_06335"
     CDS             complement(2771..3538)
                     /locus_tag="PLG52_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB41098.1"
                     /translation="MQGIHYSFQQSKETNPSRPPLIFIHGAGGSFLSFHPYLRHIQGE
                     TIYALDLPGHGKSVGKGRQSIEEYADDIARFMDDLQIASAVMVGLSMGSGIALSFALK
                     YPHKVSALVVMGGGAKLRVAQSTLENVGKPETFEATVETINRACFSTYASNELVELSK
                     KSMLSTGPDILFSDYLACNHFDVTTQLNQIRIPTLLLCGMEDIMTPPKYSQYLRDRLP
                     NAQLHLLEKTGHMVTLEQPELVAKLLKQFLDELPPLS"
     gene            complement(3551..>4414)
                     /locus_tag="PLG52_06340"
     CDS             complement(3551..>4414)
                     /locus_tag="PLG52_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB41099.1"
                     /translation="YVFPLRSGFNVYGVLLFAHPEPDFFNTERTNLLDIIGRQAVIAI
                     QNARLYQDLVEEKERLADVYEEARKKLARDLHDGPTQSVAAMAMRLNIARRMLTKDTK
                     MANEELVKLEELAHRTTKEIRHMLFTLRPLILESQGLGAAIQSMADKMMETFSQRVVV
                     EIDDRAVQQLEMGKQGVIFYIIEEAVNNARKHAAAEAITVRLRQVDTGIVLLEIQDNG
                     AGFDVKAITQNYDKRSSSSLGMINLQERAELVNGLLNIDSAPGKGTNVQVYIPLTEEA
                     ADRLHHARRSA"
BASE COUNT         1234 a         1069 c          955 g         1156 t
ORIGIN      
        1 taaccccaca aagtggacta tccttcaccg caactgtagg tgtttacagc accacgacca
       61 caaagaatgg atgcaatgat ggatatcttg taagcgagtc tcaaccttat gatggtaaaa
      121 ccatgagtgc gaatattgac tttgataaaa cttgggattt catcaataca ggtacatgta
      181 cttgggatga aggctatact tttgcatttg tgccagaatt ctcaactgcg ccactctaca
      241 ccgttccaaa ggattatata attcctaaaa atggtgtttt cactaaacca ggcgaaacta
      301 gatcattcaa agtttcttta agagtcccta aagacccagg cgaatatatc tggtattaca
      361 agatcaaaga tgatggtggt cagtattttg gttcattagt atgggtaaag gtcatttctg
      421 taagggaata aaatgaaaaa gattattttg cctcttatca tagtagtttt gctttctgcc
      481 tgtgcacctg ccacacctat cgagccaacg cccgacgtaa acgcagttcg cacatccgca
      541 gccagcacag tgatcgcgga attcacattg accgcagctt tcttcacgcc tacaccactc
      601 ccgctgccca ctgaaacccc aacgcccttc ccaacagaaa ctccggcggt tcttccttcg
      661 attgacccaa cattggcggc gttgacccca gatgctctct gcgatgatta tgattttgac
      721 ccagccacca ttgatgtgac cattcccgat gggacgccca tgacacccgg gcaagatttt
      781 gtaaaaacat ggaagatcaa aaatacaggc atctgtactt gggatacaag ttacaaagcg
      841 atcttctcat atagcaaccc gcccaatcaa cagatgagcg ctcagcccat tccgcttgcg
      901 gcactggttg ctcctggtca agaagtagat gtatctgttc agtttacagc gcctaccacc
      961 ccaggtgaat acttgggcgt ctggcaaatg gtgaatgcga aaggcattcc atttggacag
     1021 ccgggcaagc aactcactgt aaagatcgtt gttcaataat tcaatcacac taaagcaagc
     1081 tataaaagaa aaagcgcgcc agctgggatt tttcctagct ggcgttactt cttctgcatc
     1141 acttgcacat tttgatattt ttcaagaatg gctggacgcg aatcatcatg ccagcatgga
     1201 ttacctcgcc tcagaccgca gtcggttacg ccgcgcagac ccgaaacaaa ttttgcccga
     1261 atgtaaatct gttttagttc ttgcactgcc atactcccca atctcaaaaa atagttctga
     1321 atttcaaatc gcatcatatg cacttggaga tgactaccac gaagtcattc caccacgttt
     1381 gcaagagatc gtacaattta ttgagcaaca agttgggcat cctattccaa atcgctatta
     1441 cacagatacg ggtcccgtct tagaacgcga attggcgcag cgagccgggc tcgggtggat
     1501 cgggaagaat tccatgctca ttcatccgca gaaaggttct acttttctac ttgcagaaat
     1561 cttgcttggt atcgaactcg aaccggatga gccttttcaa actgatcatt gtggcacatg
     1621 cacccgttgc atggatgcct gcccaaccca atgtatcttg ccaaatcgca caattgattc
     1681 aaatcgatgt atctcctatc tcaccatcga aaacaaagtc gatattcctg aagccttgcg
     1741 acctcgaata caaaattggg tttttggatg cgacatctgt cagcaagtct gcccgtggaa
     1801 tcgattctcc ctgctagcag accctgacct caaaccatcc attccgcttc ctgtcttgac
     1861 ctctgacctg cttctgacat cctccgagtt taatcaacgc ttcaaacggt ctccaatcaa
     1921 acgcaccaag cgacgtggat atttgcgaaa ccttgctgtt gctgttggaa atcaaaaaaa
     1981 taaaaaagac ttacctctct tgcatcaagc tactcaagat gaagaaactc tcgtgcaatc
     2041 tcatgcccaa tgggcaattg aaactataaa tagaaataac acaggaaatt tatgagtaaa
     2101 cttcttattg ccaccaataa ccaagggaaa gtacgcgaac ttcaagaatt attaacgggt
     2161 tcgggtattg aattcgtgac tcctgcacag attaaccttc aaatcgatgt tgaggaagac
     2221 ggttccacct atcaagaaaa tgccggcaag aaagccatcg catttgccaa agccagcggc
     2281 ttgatctccc tcgcagatga ctcagggctg gaagtggaag cattaggtgg cgcgcctggc
     2341 ttatattccg cacggtattc cccgaaacct ggtgcaaatg ataaagaccg gcgggatttt
     2401 cttttgagca atctccaaga caagccacgc ccatggaaag cccactttca tgccactatt
     2461 gcggttgcca accctactgg tgaagttgaa tttactgatg gaaattgcta tggtgagatc
     2521 atccctgaag agcgtggtag tggcgggttt ggatatgacc ctatcttttt catgccggaa
     2581 ctcggcaaga caatgtctga actggacatg agtgagaaga ataggctcag tcatcgggcg
     2641 agggcagtga ttgcggcaat gccaatcttg aaaagaattt ttgcataaaa aaataaatag
     2701 ccagtcacac cacaagtgac tggctatttt tagattaaga aaaaactaaa tgggcagaat
     2761 cagctacccg ttaggaaaga ggcgggagct catccaaaaa ctgtttcaat aactttgcaa
     2821 ctaactccgg ctgttcaagt gtcaccatat gtccggtttt ttccagcaag tggagttggg
     2881 cattagggag acggtctctt aagtattgtg aatattttgg aggggtcatg atatcttcca
     2941 tgccacagag aagcagtgtt gggattctga tttggttcaa ctgggtagtg acatcaaaat
     3001 gattacaagc gagataatcg ctaaataaaa tatctggacc agtgctcagc atactctttt
     3061 ttgaaagttc aacaagttca ttagaagcat atgtactaaa acacgcacgg ttaatggttt
     3121 caacagtggc ttcaaaggtt tcaggcttac ccacattctc aagcgtggat tgtgcaactc
     3181 gtaattttgc tccaccaccc atcacaacca atgcacttac tttatgcgga tatttcagtg
     3241 caaaagataa agcaatgcca ctccccattg aaagcccaac catgactgct gatgcaatct
     3301 gaaggtcatc cataaaacgc gcaatatcat cagcatactc ttcaatagac tgtctacctt
     3361 tgcccacaga cttgccatgc cctggcaagt ctaacgcata gatagtctct ccttgtatat
     3421 gccgtagata tgggtgaaag gataaaaaac tgccgcccgc accgtgtata aaaatcaacg
     3481 gtgggcggct gggatttgtt tctttggact gttggaacga gtagtgaatg ccttgcacga
     3541 acggcatggc ttatgcagac ctgcgagcat ggtgcaagcg atcggcagct tcttcggtaa
     3601 gcggaatata gacctgcacg ttggtgccct tgcccggcgc ggagtctatg tttagcaagc
     3661 cgttcaccaa ttcagcacgc tcttgcaggt tgatcatacc caagctgctg ctggaacgct
     3721 tgtcataatt ttgcgtaatg gctttcacgt caaagcctgc gccgttgtct tgaatttcta
     3781 atagcacaat ccctgtatcc acttggcgca agcggacggt aatcgcttcg gcagcagcat
     3841 gtttgcgagc attgttaaca gcttcttcga tgatatagaa aatgacgcct tgtttgccca
     3901 tctccaattg ctgaacagca cggtcatcga tctcaaccac cacacgttgt gagaaagtct
     3961 ccatcatttt atctgccatg gattgaatgg cagcccccaa accttgcgac tcaagaatca
     4021 acgggcgcag ggtaaagagc atatggcgaa tttctttggt ggttcgatgt gcaagttctt
     4081 ccaatttgac gagttcttcg ttcgccattt tggtgtcttt cgttaacata cggcgggcga
     4141 tgttcaaacg cattgccatg gcagctacag actgggtagg tccatcgtgc aggtctcttg
     4201 ctagcttttt acgagcctct tcgtacacat ccgcgaggcg ctctttttct tccaccaggt
     4261 cctgataaag gcgggcattc tgaatggcga tcactgcctg acgcccaatg atatccaaaa
     4321 gattagtccg ttcggtatta aaaaaatcag gttcagggtg agcaaacaaa agcacaccat
     4381 atacgttgaa accgcttcgt aaagggaaaa cata
//
LOCUS       DAOQNJ010000174         4411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12400_length_4411_cov_5.782369, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000174 DAOQNJ010000000
VERSION     DAOQNJ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12400_length_4411_cov_5.782369"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLG52_06345"
     CDS             <1..563
                     /locus_tag="PLG52_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNB41100.1"
                     /translation="LGEHQLPSGKGTPYEEDIRVPFLLRGPGITPGTTVTQMTANIDL
                     APTIAAMAGAEVPDFVDGRSLLPCLEESNLQWRNGLLIEFGYIDEEAIAEALADPETD
                     NLLVDVAGGAFRGIRGEDFVYVEYANGEIEFYDLTNDPYQLENLAGTLSADTLEILHT
                     KLETLKYCAGSDCQMLEDDLKIEFQK"
     gene            595..1251
                     /locus_tag="PLG52_06350"
     CDS             595..1251
                     /locus_tag="PLG52_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNB41101.1"
                     /translation="MIVLSDMNGTLTTGSPFLGVVDWVRHNQNKFEATWYKVSILPSY
                     FLAKRGWIDWQEWGQGLMIDSLGYIKDATPEKIAQVSEWLVEHELWKNRREDVVARLV
                     KHREEGAKTYIASSMMEPFLAPFARRIGAEVIGTPTEIVNGRLKLVGDMVVNEKKIEQ
                     VLSRLAVDRVDIAYGDSFMDIPMLEHADHPVAVYPDEKLKKLAQERGWEILGNTGWYE
                     "
     gene            1356..2099
                     /gene="budA"
                     /locus_tag="PLG52_06355"
     CDS             1356..2099
                     /gene="budA"
                     /locus_tag="PLG52_06355"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNB41102.1"
                     /translation="MERTTIRRSRKRRAESKIYLCAPVNSLVEGIYEQNIPFHEIRKH
                     GDMGLGTFNDLDGEMMMVDGEIYRVGSDGKVNLITDDDTCTPFACVTFFQPASDEVLE
                     KEMDYEQFLRWMMELMPSPNIFYAFRITGEFAYVKTRSVPKQENYRPLVEVAKEQPTF
                     EFHNVSGKMVGFYTPSFVSALSVPGVHLHFLTEDKTSGGHLLACHPRKVKVELQYLYT
                     VELSLPANLDYLTWDFQRDIGKDLDKAEK"
     gene            2109..2585
                     /locus_tag="PLG52_06360"
     CDS             2109..2585
                     /locus_tag="PLG52_06360"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNB41103.1"
                     /translation="MKRPNWIVIAILFGVSLFLGYAIFTALATAIFPAVNNVSGEFLC
                     DGNYEIITTKSSPRPGQTYWSHSILCDGKDITLPTVAITGLMASSIFFVLLLIFARNW
                     WFRAASAPAPFAKAGVTEKGKKSTLDRMAELKEMRDKNLISQVEYERKKDDIMKEL"
     gene            2582..3013
                     /locus_tag="PLG52_06365"
     CDS             2582..3013
                     /locus_tag="PLG52_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41104.1"
                     /translation="MMHKFDEFIHRELAPHLSAGEGIDLTGFLYTKSLRGVVLSGGLS
                     LVGDGYYFAALTRKRMFIIATSMGIASLNMENKGLSDIPYAEIKSIKPSGFLNQKMIA
                     IELKDGKIVVFRLNSLATHYASGQKQFIETLIQYVGAGLVP"
     gene            complement(3073..4134)
                     /locus_tag="PLG52_06370"
     CDS             complement(3073..4134)
                     /locus_tag="PLG52_06370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB41105.1"
                     /translation="MNFEGKLGLQQRVLPSYRVPFFDLLAGHCEGDMSLFAGQARREE
                     MIASGTTRIARHVAADNLHLLSGPLYFCYQKNFIRWLEEWNPDALIMEANPRYLASPS
                     AVSWMHKRRRKALGWGLGAPKQSGLWKRFIHQFDGMIAYSQRGAEEYAALGFPREKIF
                     VAHNSVAAAPEVPDRRPQTVDRVTILFVGRLQARKRVNSLLRACAEMEVKPRLLIVGD
                     GPEHDALEILAKEIYPSAEFIGAKHGEELKPYFQQADLFVLPGTGGLAVQEAMSYGLP
                     VIVAKGDGTQDDLVREKNGWQIEPENYEALVSTMKNALSDIARLREMGKESYRIVSEE
                     VNIEKMAGTFVRALNSIVA"
     gene            complement(4191..>4411)
                     /locus_tag="PLG52_06375"
     CDS             complement(4191..>4411)
                     /locus_tag="PLG52_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB41106.1"
                     /translation="ETGKTRFDLSNLLIQRYGDTAIASYTLLISTALGTGVQVAAHNE
                     SRVMVKLNGNWKVVHVHKSPAYSAPHVG"
BASE COUNT         1093 a         1075 c         1122 g         1121 t
ORIGIN      
        1 accttggcga acatcaactg ccatctggca agggcacacc atatgaggaa gacatccgcg
       61 ttccgttcct gctacgcgga ccgggcatca cgccgggcac gactgtgact caaatgacag
      121 ccaatattga tctcgctcct accattgcag caatggctgg cgccgaagtc ccagactttg
      181 tggatgggcg ttccttatta ccatgtttgg aagagagcaa cctgcaatgg cgtaacgggc
      241 tgttgatcga atttggctac atcgacgaag aagccattgc tgaagccctc gccgacccag
      301 agaccgataa cctgcttgtc gatgtggcgg gtggtgcctt ccgtggcatt cgcggtgagg
      361 attttgtcta tgtggagtat gccaatggcg aaatcgaatt ctacgacctg accaatgacc
      421 cctatcaact ggaaaatctg gctggtacat taagcgccga tacgctggag atacttcata
      481 caaaattgga aactttgaaa tattgtgcag gttcagattg tcagatgttg gaagatgatt
      541 tgaagatcga atttcaaaag taggtcaggc ttcaactctg acacaggaga ttaaatgatc
      601 gttttatcag atatgaacgg aaccctgacc accggttcgc cctttctcgg tgtggtggat
      661 tgggtcaggc ataatcagaa caaatttgag gctacttggt ataaggtatc catcctgccc
      721 tcgtatttcc ttgccaaacg cgggtggatc gactggcagg aatggggtca ggggttgatg
      781 atcgacagcc tcggttatat caaggatgcc acgcctgaaa agatcgctca ggtttcggaa
      841 tggctggtgg agcatgagct gtggaagaat cgccgcgaag acgtggtggc gcggctcgtc
      901 aaacatcgtg aagaaggcgc gaagacctat atcgccagca gtatgatgga gccgttcctt
      961 gcaccgtttg cgcggcgcat tggcgcggaa gtgatcggca cgccgactga gatcgtcaat
     1021 ggacggttga agctggtggg tgacatggtc gtgaacgaga agaagatcga gcaggttttg
     1081 agtcgtctcg ctgtggatcg agtggatatc gcttatggtg attcattcat ggatattccc
     1141 atgttggaac atgccgacca tcctgtggcg gtctaccctg atgaaaaatt gaagaagctc
     1201 gcacaggaac gcggttggga gattcttggc aatacgggtt ggtatgaata gcactgtcat
     1261 tgcggcgaat caatcttatg attaagtctg agattgcttc gccgcaaagt acaagcgcgc
     1321 ggctcgcaat gacataaaag gagaaaaaaa ttcaaatgga acgaacaacc attcgcaggt
     1381 ccagaaagag gcgggcagag agcaaaatct atttatgtgc gccggtcaat tcgttggtcg
     1441 aaggcattta tgagcagaac atcccattcc atgagattcg caaacatggc gatatgggct
     1501 tgggcacctt caatgacctt gacggtgaga tgatgatggt ggatggcgag atctatcgcg
     1561 tcgggtcgga tggcaaggtc aacctgatca ccgatgatga tacctgcacg ccatttgctt
     1621 gtgtcacttt ttttcagcct gccagtgatg aagtgctcga aaaagaaatg gactatgagc
     1681 agttcctgcg ttggatgatg gaattgatgc cttcaccgaa cattttttat gcatttcgca
     1741 ttacgggtga atttgcgtat gtcaaaacgc gctcggtgcc caagcaggag aattatcgtc
     1801 cgctggtgga ggtggcgaag gaacagccga cctttgaatt ccataacgta agcggaaaga
     1861 tggttgggtt ttatacgcca tccttcgtct ctgcgcttag tgtaccgggc gtgcatttgc
     1921 attttctcac cgaagataaa accagtggcg gacatttgct ggcatgtcat ccgcgcaagg
     1981 tgaaggtgga attgcaatat ctttatacgg tagaactttc gttgcctgca aatcttgatt
     2041 accttacttg ggatttccag cgcgatattg gcaaagattt ggataaagca gagaaatagg
     2101 aggttgttat gaaaagaccc aattggatcg tcatcgcaat attgtttggc gtcagtttgt
     2161 ttttaggata tgccattttc acggcgttag ccactgcgat ctttccggcg gttaacaatg
     2221 tttctggtga attcctatgt gatggtaact atgagatcat caccacaaaa tcttctccgc
     2281 gccccggtca aacctattgg tctcatagca tcctctgtga tggaaaggac atcacgcttc
     2341 ctacagttgc gataacgggt ttgatggcat cctctatatt ttttgtgctg ttgcttatct
     2401 tcgcgcgtaa ttggtggttt cgtgctgcat ctgccccagc tccatttgcc aaagccgggg
     2461 tgacggaaaa aggaaaaaaa tctacgctcg atcgtatggc agaactgaag gaaatgcgcg
     2521 acaagaacct gatctcacaa gtggaatatg agcgtaaaaa agatgacatc atgaaggagc
     2581 tatgatgcat aagtttgacg aattcatcca tagggaactc gcgcctcatc tctccgccgg
     2641 agaaggcatc gacctcactg gctttctcta caccaaatcc ttgagaggcg ttgtcctctc
     2701 tggcggtttg agcctggtcg gtgatggata ctatttcgcc gcccttactc gtaagcgcat
     2761 gttcatcatt gctacttcga tgggaattgc ttctctcaac atggaaaaca aaggcttgag
     2821 tgatattcct tatgcagaga tcaaatccat caaaccaagc ggattcctca accagaaaat
     2881 gatcgccatt gaactcaaag acggcaagat tgtagtcttc cgcttgaact ctctggcgac
     2941 gcattatgct tcaggtcaga agcaatttat tgagacattg attcaatatg taggggcggg
     3001 gcttgtgcca tgaagtggta tctccccgcc ccaattaaag gggaaataaa aaagggcgcg
     3061 gttctcgcgc ccctacgcaa caatcgaatt gagagctctc acaaacgttc ctgccatctt
     3121 ctcaatattg acctcttccg aaacaattcg atacgactct ttccccatct ctcgcagacg
     3181 cgctatatcc gatagcgcgt ttttcattgt agagaccaac gcttcgtaat tctctggctc
     3241 gatctgccag ccgttcttct cgcggactag gtcatcttgc gtgccatcgc cttttgccac
     3301 gatgacggga agtccatagc tcatcgcttc ttgcacggcg agtccgcctg taccggggag
     3361 gacgaacaaa tctgcttgct ggaagtaagg cttcaactcc tcgccgtgtt ttgcgccgat
     3421 gaactcagcg gatggataga tttcttttgc taatatttcc aatgcgtcgt gttccggtcc
     3481 atcgccaaca ataagcaggc gcggcttgac ttccatttca gcacaagcgc gcaataatga
     3541 attaactcgt ttgcgggctt gaagtcgtcc cacaaaaaga atcgtcacac ggtccacagt
     3601 ctgcggtctg cggtcaggta cttcgggtgc cgccgccacc gagttatgtg ccacaaaaat
     3661 cttctcacgc ggaaaaccca atgcagcata ttcctccgcg ccgcgttgac tgtaggcgat
     3721 catcccgtcg aattgatgga tgaagcgctt ccagagtccg ctttgtttgg gggcacccaa
     3781 accccagcct aaggcttttc gtctgcgttt gtgcatccaa ctcacggcgg agggcgaggc
     3841 gaggtaacgc ggattggctt ccatgatgag cgcgtcggga ttccactctt cgagccagcg
     3901 gatgaaattt ttctgatagc aaaaataaag cgggccgctg agcaaatgca aattatccgc
     3961 cgcaacatgc ctggcaatcc tggttgtacc actcgcaatc atttcttcgc gccgagcctg
     4021 ccccgcaaaa agactcatgt caccttcgca atgacctgcc agcaagtcaa agaatggcac
     4081 gcgataactc ggcaagacgc gttgctgcaa ccccagtttg ccttcgaaat tcatttcacc
     4141 tcacgcttga aaaatatccg tttcatccgc ttatccgatt caacaatccg ctaccccaca
     4201 tgcggcgcag agtaagccgg tgatttatgg acgtgtacca ctttccaatt tccattcagc
     4261 ttcaccatca cacggctttc attatgcgcc gccacctgca cgcccgtccc caatgcggtg
     4321 ctgatgagca gggtataact cgcaatggcc gtatcgccat accgttgaat caataggttt
     4381 gataagtcaa aacgtgtctt gccggtctct t
//
LOCUS       DAOQNJ010000175         4403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12443_length_4403_cov_6.479761, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000175 DAOQNJ010000000
VERSION     DAOQNJ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12443_length_4403_cov_6.479761"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLG52_06380"
     CDS             <1..721
                     /locus_tag="PLG52_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HNB41107.1"
                     /translation="LRNTVLSVTGTGYEVENYIWTFPLWTTFYVRAGWTVHVEKTSGG
                     NWAYIAIAGGFDSPVIMNSRSVYLRGRIGSALKAGDVLHSFTPTTDLLKLAARDFPVN
                     KFMPYRQTPTIEVISAPQADWFTPEGIRTFYDSEYTLSPSFDRMGYRLQGPPIEKAIT
                     KELISEGMTIGSIQVPDNGQPIIMMADAPTAGGYPKIANVARASLPLLAQCEPGMSRI
                     RFREITVEEAQDALQKTSEVF"
     gene            748..1452
                     /locus_tag="PLG52_06385"
     CDS             748..1452
                     /locus_tag="PLG52_06385"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNB41108.1"
                     /translation="MKIDLNCDLGEGMDNDAAIMPYITSANIACGFHAGDKHTMRETL
                     RLAKRFGVNAGAHPSWDDRENFGRREMDVFPKEAEKLVFEQIQILAEMAKEKGVTLTH
                     VKPHGALYNQSAKDIELATAIARAIKAVSVDLILVGLAGSRSIEAGRAIGLRVAAEGF
                     PDRGYNADGSLMSRLLPGALIESPEDVAKHAIELVKTGKMDTLCLHGDHPNAAENAKK
                     LREVLEKNRVEVVGLT"
     gene            complement(1551..2870)
                     /locus_tag="PLG52_06390"
     CDS             complement(1551..2870)
                     /locus_tag="PLG52_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41109.1"
                     /translation="MSADYAKELLRSGITDAKTGSKESARRYLDRAIYMAGSHDVLAE
                     AWYWMSEVVDDPAEKRKALENCLSHDLHHTRARRSLAILDGKLKAGDVINPDKLPAAP
                     EGLREAQADRFMCPKCGGRMAFSPDGSGLVCDYCTRQNPLGAGSGTENEKDFIVAMAT
                     MKGHGKPLQEQVFHCNGCGAEFILPPNQISSNCAYCDSPHVVSYEKTNDIIAPNSILP
                     FAIDQKRATHLLVEWVEKNHIKPEKKVDLPRGVYLPMWTFDLGGEIDYSGEIVEYEQN
                     LFTGERERKVTRVEDKYPVMVNDLALPASRKLSQVFMKLLPTFDLAALKEYDPRYLAS
                     WLAELYDVPMADASLDARSQAFKKLKKQLPHLLGNITLTRASSASMLVESFKLVLLPV
                     WMTELPFDGREHIVLINGQSGVVASDLSEKDEEHGGLFDFLADLLND"
     gene            complement(2860..3750)
                     /gene="folP"
                     /locus_tag="PLG52_06395"
     CDS             complement(2860..3750)
                     /gene="folP"
                     /locus_tag="PLG52_06395"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNB41110.1"
                     /translation="MKSTTLQVGSFTFDWGSRTYVMGILNVTPDSFSGDGIIAKGDVV
                     KAAVEQAREFILYGADILDVGGESTRPGSEPVNAEEEMERVIPVIEELRKNFPQVLIS
                     VDTYKAKVAEAAIQAGANIINDVWGLRADSELASVAAKHKVPVILMHNRSNPASVEVR
                     EKLGGTYLGAEYDDLLEDVKRELLASVEIAVKAGVEEKLIMLDPGIGFGKKREHNLEL
                     INRLAEIRSLGYPILLGTSRKSFIGFTLDLPPDQRVEGTAATVAVGITRGADIIRVHD
                     VKEMVRVAKMTDAMVRVNER"
     gene            complement(3804..4226)
                     /locus_tag="PLG52_06400"
     CDS             complement(3804..4226)
                     /locus_tag="PLG52_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41111.1"
                     /translation="MSDPKPLNWTDTPGIGYSVVRRGDGGMTLTFTDLSDATMQHWHQ
                     FALDHLLGADRRTRNLYDLRSVPEIPEKAIRMAVDANSDPSARNIRVAVVVASEAVAD
                     GIRKVAALAETESAAAMKIFTDINEAEAWLARPLDQIA"
     gene            4321..4394
                     /locus_tag="PLG52_06405"
     tRNA            4321..4394
                     /locus_tag="PLG52_06405"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4355..4357,aa:Val,seq:tac)
BASE COUNT         1107 a         1147 c         1081 g         1068 t
ORIGIN      
        1 attacgcaat acagtccttt ccgtcactgg cacaggctac gaagtagaaa actacatctg
       61 gaccttccca ctctggacga ccttctacgt ccgcgccggg tggacggtgc atgtggaaaa
      121 gacaagtggc ggcaactggg catatatcgc catcgcaggt gggttcgatt ctcccgtcat
      181 catgaattcg cgttctgtat acttgcgcgg cagaatcggc tccgctctca aagctggtga
      241 tgttctccat tccttcactc ccaccaccga cctgctcaaa ctcgccgccc gcgactttcc
      301 tgtaaacaag ttcatgccct accgccaaac acccaccata gaagtcatct ctgcccccca
      361 agcggactgg tttacacctg aaggcatccg taccttttat gatagcgaat acaccctcag
      421 cccttccttt gaccgtatgg gctatcgctt acaaggtcca ccaatcgaga aagctatcac
      481 caaagaactc atctccgaag gcatgacaat tggcagcatc caagtcccgg acaatgggca
      541 gcccatcatc atgatggcag acgctcccac tgcgggcggt tatcctaaga tcgcaaacgt
      601 ggcacgcgca agtctgcctt tgctggcgca gtgcgaacca ggtatgagca gaattcggtt
      661 tagagaaata actgtggaag aagcacaaga cgcattacaa aagacctccg aggtttttta
      721 aacctcggag gtctgattgg agatactatg aaaatcgatc tcaactgtga cctcggcgaa
      781 ggaatggaca atgacgcagc catcatgccg tacatcacct ctgcaaacat cgcttgcgga
      841 ttccatgcag gtgataaaca taccatgcgc gagacacttc ggctggcaaa gcgctttggt
      901 gtgaatgcgg gcgcacaccc aagctgggat gaccgtgaga attttgggcg cagggaaatg
      961 gatgtttttc ccaaagaagc ggagaaatta gtctttgagc aaattcaaat tctggcagag
     1021 atggcaaaag aaaaaggcgt aacactgacc cacgtcaaac cgcatggggc gctgtacaac
     1081 caatcggcaa aagatattga attggcaacg gcaattgcgc gggcgataaa agctgtcagc
     1141 gtggatttga ttttagttgg tctggcaggt tcaaggtcga ttgaggcggg gcgggcgata
     1201 gggctgagag ttgcagcgga gggtttcccc gatagaggct acaacgctga cgggtcgttg
     1261 atgtcccgcc ttcttccggg tgcgttgatc gagtcaccgg aggacgtggc gaaacatgca
     1321 attgagttag taaaaaccgg gaagatggat acgctctgcc tgcatggcga ccacccaaat
     1381 gcggcagaga atgcgaagaa gctgagagaa gttttagaga aaaatagggt tgaggttgtg
     1441 ggattaacgt aggggcgacc cagatacatt taatgaaaac tttttgcaaa aggcgaccct
     1501 tactttgaac tttgatttca gctcaaatct tgacggggcg cccctacaaa ttaatcattc
     1561 aaaaggtctg ccagaaaatc gaacaagccg ccatgttctt catctttttc agataaatca
     1621 ctggcaacta cgccgctctg accattgatg agcacaatat gttcgcgtcc gtcaaaagga
     1681 agttcggtca tccacacggg caggagaacc agcttgaagg actcaaccaa catacttgcc
     1741 gatgatgcgc gtgtaagggt gatgttgcca agcaaatgcg gcaattgctt cttgagtttc
     1801 ttaaatgcct gactgcgcgc atccagagag gcatctgcca tggggacgtc ataaagctca
     1861 gccaaccagc ttgcgagata acggggatca tactctttga gtgcggcaag gtcaaaggtg
     1921 ggcaaaagct tcataaacac ttgagacaat tttcgagaag cgggaagcgc aaggtcattc
     1981 accatgacgg gatatttatc ttccacacgt gttactttgc gttcacgttc tccggtaaaa
     2041 aggttttgct cgtattcaac gatttcccct gaataatcaa tctcgccacc aaggtcgaag
     2101 gtccacatgg gaagatacac accacgagga aggtctactt tcttttctgg tttaatatga
     2161 tttttttcga cccattcaac cagcaaatgt gtagctcgtt tttggtcgat ggcaaaaggc
     2221 aaaatggagt tgggcgcaat aatgtcgttc gtcttttcgt agctgacgac atgtggcgag
     2281 tcgcagtagg cacagttcga agaaatttgg ttgggtggca aaataaattc tgcaccacaa
     2341 ccattgcaat gaaagacttg ttcttgcaat ggtttgccat gtcccttcat ggttgccatt
     2401 gctacaatga aatctttttc gttctcggtt ccgctgcctg cccccaacgg attctgacgc
     2461 gtacaataat cacacaccaa cccagaacca tcgggcgaga acgccatgcg tccaccgcac
     2521 ttggggcaca tgaaacggtc tgcttgcgct tcgcgcaacc cttctggcgc ggcaggcagt
     2581 ttatcagggt tgatcacatc gcccgcttta agtttgccgt ctaaaatcgc gagcgagcgg
     2641 cgcgcacgcg tatgatgcaa gtcatgcgaa agacaattct ccaatgcttt gcgtttctca
     2701 gcagggtcat ccaccacttc gctcatccaa taccaagctt ctgccaacac gtcatgcgaa
     2761 cctgccatgt aaatggcgcg gtctaaatac cggcgggctg attctttact gccggttttg
     2821 gcatcggtaa tacctgaacg aagaagttct tttgcgtaat cagcgctcat tgaccctcac
     2881 cattgcatcc gtcatttttg ccacacgcac catctctttt acatcatgca cacggataat
     2941 atccgcacca cgcgtaatac ccaccgccac ggttgccgcc gtcccttcca cgcgctggtc
     3001 gggcggcaag tctaatgtaa aaccaataaa tgattttctg gatgtaccca atagaattgg
     3061 ataccctaac gagcggatct ctgcgagtcg gttaatcagc tcgaggttgt gctcgcgctt
     3121 ctttccaaag ccgatgcctg ggtcaagcat aatgagtttt tcctccaccc cggctttaac
     3181 agcaatctct acactggcaa gtaattcacg tttcacatct tctagcaaat catcatactc
     3241 tgcgccgagg tatgttccgc ccaatttctc gcggacttcc acactggcgg ggttgctgcg
     3301 attgtgcatg agaatgactg gcactttatg cttcgccgcc accgatgcga gttctgaatc
     3361 tgcacggagc ccccacacat cgttgatgat gttcgcgccc gcttgaatgg ctgcctctgc
     3421 aactttggct ttgtaagtat caacagaaat caaaacctgc gggaaatttt ttcgcaattc
     3481 ctcgatgact ggaatgacac gttccatttc ttcttcggca ttgaccggct ccgaacccgg
     3541 gcgcgtggat tcgccgccca catcgagaat gtccgcaccg tagaggatga actcacgcgc
     3601 ctgttccact gccgctttga ccacatcgcc tttggcaatg atcccatcac cagaaaacga
     3661 atcgggagtc acattcaaga ttcccatcac ataggtgcga gaaccccaat cgaatgtaaa
     3721 actcccgact tgaagggttg tagatttcat gtgtataaaa ggctcttgat gaggttaaac
     3781 ctcatcaaga gcgaatttgt aaattaagcg atctggtcca acgggcgtgc aagccaagct
     3841 tccgcttcgt tgatgtcggt gaatattttc atcgccgccg cagactcggt ctctgccaat
     3901 gctgctactt tacgaatacc atctgccacg gcttcgctcg ccaccactac cgccacacga
     3961 atgttgcggg ctgaggggtc actgtttgcg tctaccgcca tgcgaatggc tttctctgga
     4021 atctcaggaa cagaacgcag atcgtagaga ttgcgcgtac ggcggtctgc gccaagaaga
     4081 tggtcaaggg cgaattgatg ccagtgttgc atcgttgcat cagaaaggtc ggtgaacgta
     4141 agggtcatgc caccatcgcc gcgtcggacg actgaatagc cgattccggg ggtatctgtc
     4201 cagttaagag gtttggggtc gctcatgagt ggtctcctat tttattaatc cattataccc
     4261 agcgttggca aaaaatcgtt tgcgttaatt ttcaaatcac gtgctataat gcccgtcgcc
     4321 gggtgcttag ctcagttggt tagagcactt cgtttacacc gaaggtgtcg ggggttcgag
     4381 tccctcagcg cccacaaaaa agc
//
LOCUS       DAOQNJ010000176         4381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12539_length_4381_cov_8.395284, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000176 DAOQNJ010000000
VERSION     DAOQNJ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12539_length_4381_cov_8.395284"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..827)
                     /locus_tag="PLG52_06410"
     CDS             complement(39..827)
                     /locus_tag="PLG52_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41112.1"
                     /translation="MFRRKITLFVCIFLVGALSACNLPSSAPTETETSELDLALTITA
                     LASSPNAPQETSTPEFTATAGPTSTPSVPQVSVSQNTNCRVGPGIVYDLIDSLLIGQT
                     AEIVGKNSGVPNYWVIKRINGSGTCWLWGEYATVTGNTANLPEYPVPPTPTPSPTPTF
                     TPTATPSAPAAVENVTMTMSTCGGVAPYQHGGTLAWEDKSNNEDGFNIYVNNVLIASI
                     PANSTSYNVAPAGSFAAGIASKFEVEAFNGVGKAAKKSVAKGCP"
     gene            complement(944..1684)
                     /locus_tag="PLG52_06415"
     CDS             complement(944..1684)
                     /locus_tag="PLG52_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNB41113.1"
                     /translation="MTRIAVISDIHGNILALEKVVQDIKRRGVDQVVNLGDHVSGPLW
                     AKETIQFLMEQSWIQIRGNHDRQLVSQAPETHGASDKSAFQQLNENELDWLRKLPPQA
                     ELLGEILFVHGTPKSDNTYLLETIAHGSNRIATRNEIKERIGDSQHKLILCGHTHIPR
                     VVAISEQTTIINPGSVGLQAYDDTSPEYHIMQTGSPHARYVILEKVDINWVVNLLHVE
                     YDHNSAAAHAHKNGRIEWEYALRTGLMP"
     gene            complement(1681..2532)
                     /gene="rsmA"
                     /locus_tag="PLG52_06420"
     CDS             complement(1681..2532)
                     /gene="rsmA"
                     /locus_tag="PLG52_06420"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNB41114.1"
                     /translation="MNYSSIPPLNAAAVLKRYGLHADKRLGQNFLQDHFALEKIATAA
                     EIQADDCVLEIGPGLGSLTRYLAVSARQVTAVELDPDLLNPLKAVLTPHPNVRIVHGD
                     ILDLRVADLVDQSGYIVAANIPYNITSAIIRHLLESDPKPRRIVLTIQKEVAERICAK
                     PGDLSLLALSVQVYGSPSIQSKIPAGAFHPIPNVDSAILRIDIYDQPLISAEMLSTFF
                     KLIKAGFSQKRKTLRNSLSSGLHIKPQDAETLLNKSNIDPMRRAETLSIAEWKGVCET
                     MAQGNSK"
     gene            complement(2529..3671)
                     /locus_tag="PLG52_06425"
     CDS             complement(2529..3671)
                     /locus_tag="PLG52_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNB41115.1"
                     /translation="MTKPQRITIAVLSVIAFLLAAVLGVYVYQSLKFVAQTPLGPALP
                     SIPTQSMPPTWTPSPGPAATQMGSVTLAPTLSLPATPSGPLCGGPPLMNVLVIGADTR
                     GDNYTYGLGDAIRLVRVDFTTPKVSVLEFPRDLWVEIPHIEDNLNGQDHEKLNQAYLY
                     GQPGFKYWDDPSEGSGLTALTLNLNFGANIDHYITVNMRTFEHVIDAMGGIEVEIPNE
                     DVAKTSGLSVGTHTLNGPQALKLARNRSGGIFERADNQNLVLCAVRKKLTSPQIVAQI
                     PALIETFQDNIRTDFTPEQLGQFACLATKLPGENILLASFPGELFKTTRVFDPVFDKR
                     ISIVDADFEILRDFVTRFQAGTWPVPVSADTTSTGIDNEDAASVCE"
     gene            complement(3673..>4381)
                     /locus_tag="PLG52_06430"
     CDS             complement(3673..>4381)
                     /locus_tag="PLG52_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNB41116.1"
                     /translation="AGIPLMGMYTSVPAENEPLPIDGQITVTRVTESVLVELQSIPFS
                     TEETESVDVAFGEQNILQTGENGIAMVKTRIRYEYGVEVNRVEEDKTILKEPVKQIVA
                     KGTKIVLSEVGGSAPYQYWYATQMYASWYSPCNSGTGGCSYGTASGAKAGFGIVAVDY
                     SYYSYLAGMRVYIPGYGVATIGDTGGGPVIESTFGVPRYQWIDLGYDDNNIGGLSGWV
                     TVYFLEPAPVEIPYFFK"
BASE COUNT         1121 a          939 c         1097 g         1224 t
ORIGIN      
        1 tctggcaggg atttccctgc cagacttatg agaaaatttt atgggcaacc ctttgcaaca
       61 ctctttttag ctgcctttcc aacaccattg aaggcttcca cttcgaattt tgaagcaatt
      121 cctgcggcaa acgagccggc tggtgcaaca ttgtacgaag tgctattcgc tgggatgctg
      181 gcaattaaga cattgtttac ataaatattg aaaccgtctt cattgttgga tttatcttcc
      241 catgctagag taccaccatg ttgatagggg gctactccac cacaagttga catggtcatt
      301 gtcacgtttt caactgcggc aggcgctgaa ggtgttgctg taggtgtgaa tgtaggcgtc
      361 ggtgaaggtg tcggtgtagg aggaaccgga tattctggca gattcgcagt gtttcccgtt
      421 acggtggcgt actcgcccca cagccaacaa gttccagatc cattgatgcg tttgatgacc
      481 caataattgg gaacgccaga atttttacca acgatttctg ctgtttgccc aatgagcagg
      541 ctatcaatta aatcatagac aatgccagga cctacacggc aatttgtatt ttgactaacc
      601 gatacctgtg gaacagaggg tgtagatgtg ggacccgctg tggcagtaaa ctctggcgtg
      661 gaggtttcct gcggtgcatt cggactgctg gcaagagccg taatcgtgag tgcaagatcg
      721 agttctgaag tttcagtctc agtgggagca ctgcttggaa ggttacaagc agaaagagca
      781 ccaactaaga atatacaaac gaataaagtt atttttctgc gaaacataat tctctccttt
      841 ataagaaact attatggcac aactttgata tatatcaata aaagggcttg tgagattagt
      901 actagtgtgc aaagggacgt tggtactagt actagctttt gttctagggc attaaaccgg
      961 ttcttaacgc atattcccat tcaatccgcc catttttgtg agcgtgtgct gcagcagagt
     1021 tatggtcata ctcaacatga agtagattca cgacccaatt aatgtcaacc ttttcaagaa
     1081 ttacatacct tgcatgtggc gaacctgttt gcatgatgtg atattctggg ctggtatcat
     1141 cgtatgcttg aagccccaca ctaccggggt ttatgatcgt agtctgttct gagattgcaa
     1201 ctactctggg aatatgagtg tgtccgcaga gaataagttt gtgttgagaa tcaccaattc
     1261 gttcttttat ctcattccgt gtagcaattc tattgctgcc atgagcgatg gtttcaagta
     1321 ggtaggtgtt atcacttttt ggtgttccat ggacgaatag aatttcgcct aacaactctg
     1381 cttgaggagg tagtttgcga agccaatcta attcattttc attcaattgt tgaaaggcag
     1441 acttatcaga ggctccatgt gtttctggag cttgtgagac gagttgtctg tcgtggtttc
     1501 cgcggatttg gatccagctc tgttccatca agaattggat ggtttccttc gcccaaagcg
     1561 gacctgatac atggtcgccc aagttgacga cttgatctac acctcgtctt ttaatatctt
     1621 ggacaacttt ttcaagggca aggatgtttc catgaatatc tgaaatgaca gcgattcttg
     1681 tcatttgcta ttcccttgag ccatggtttc gcatacccct ttccattctg cgatggataa
     1741 agtctcagcc cgacgcatgg ggtcgatatt tgatttatta agtaaagtct ctgcgtcttg
     1801 tggtttgata tgtagcccag aagagagtga atttcgtaac gttttgcgct tttgactaaa
     1861 accggctttg atgagtttga agaatgtgct tagcatttct gcagagataa gcggttggtc
     1921 gtagatatca atgcgcagaa tagcagagtc cacattgggg atgggatgga atgccccagc
     1981 ggggattttg gattggatgc ttggcgaacc gtaaacttgt acgcttaatg ctaaaaggct
     2041 taagtctccg ggtttggcgc agatgcgttc ggcgacctct ttttgaattg tcaacacaat
     2101 gcggcgcggc tttgggtcgc tttctaatag gtgacgaatg atcgccgagg taatgttgta
     2161 aggaatatta gctgcaacaa tataaccact ttggtcgaca aggtcagcga cacgaagatc
     2221 taaaatatca ccgtgaacga tgcgcacatt cggatgcggt gtaagaacag ctttgagtgg
     2281 gttgagcagg tctgggtcca gttcgaccgc tgtaacttga cgggcagaga cagctaaata
     2341 gcgtgtaaga ctgccaagtc cgggaccaat ttccaagaca cagtcatccg cttgaatttc
     2401 agcagcagta gcaatttttt cgagggcaaa atggtcttga agaaaatttt gtcctagacg
     2461 tttatcggca tgcagcccgt aacgttttaa gacggcggca gcattaagtg gggggatgga
     2521 ggaatagttc attcgcagac agaagcggca tcttcgttgt caatgccagt ggatgttgta
     2581 tcagcagaaa cagggacggg ccaggtgcct gcttgaaaac gggtaacgaa atctcgtaag
     2641 atttcaaaat ctgcatctac gatggagatg cgtttatcaa aaaccgggtc gaagacacgt
     2701 gtggtcttaa atagctcgcc ggggaaacta gcaagtagga tattttcgcc gggcaatttt
     2761 gtggcaaggc atgcgaattg acccagttgc tcgggcgtga aatcggtgcg gatgttgtct
     2821 tgaaatgttt cgatcaaggc agggatttgc gctacgatct gagggctggt taattttttg
     2881 cgtactgcac aaagcacaag gttttgatta tcggcgcgtt cgaaaatacc accagagcga
     2941 ttacgtgcta atttcaaggc ttgtggaccg ttgagggtgt gtgttcccac agagagtcca
     3001 gaggtctttg ccacatcttc attaggaatt tctacctcga taccacccat ggcatcaatg
     3061 acatgttcaa aggtgcgcat gttgacggtt atatagtggt caatatttgc accaaaattt
     3121 aggtttaggg tgagggcggt tagccctgaa ccttcactgg ggtcatccca atacttgaag
     3181 ccgggctgac catatagata ggcttgattt aatttttcat ggtcttgccc gttgagattg
     3241 tcttcgatgt gcggaatttc cacccacagg tcacgcggga attccaacac acttactttt
     3301 ggggttgtga aatccacccg cacaaggcga atggcatctc caagcccata ggtgtaatta
     3361 tcgccacgag tatcggcgcc gatgacgagc acattcatca atggaggtcc gccgcacaat
     3421 ggacctgatg gcgttgctgg caaagaaaga gttggtgcaa gcgtgacaga ccccatttgc
     3481 gttgctgccg gaccaggaga tggtgtccaa gtggggggca tggattgggt cggaatcgaa
     3541 ggcaaggcag gtcccagcgg agtttgtgct acaaatttaa gtgattgata cacataaacg
     3601 cctaaaaccg ctgccagtaa aaatgcaata acggatagta cagcaatagt gatacgttga
     3661 ggttttgtca tattatttga aaaaataagg gatttctacg ggcgcaggct ctaaaaagta
     3721 tacagtcacc cagccagaga gaccgccaat gttattatcg tcatagccga gatcgatcca
     3781 ttgatatcgc ggaaccccaa aggtcgattc aataaccggt cctccgcctg tatcgccaat
     3841 agttgcgacc ccataaccag gaatgtaaac gcgcatcccg gctaaatatg agtaatatga
     3901 ataatcgact gctacaatgc cgaacccggc tttggctccg cttgcagtgc cgtacgaaca
     3961 tccacctgtg cctgagttgc atggcgaata ccatgaggca tacatttggg ttgcgtacca
     4021 atattgatat ggcgcgctac cgccaacttc tgaaagtaca atctttgtcc ctttggctac
     4081 aatttgtttc acgggttctt tcaaaatagt tttatcttct tcaactcggt tcacttccac
     4141 accatactcg tatcggatgc gagttttcac catggcaata ccgttttccc ctgtttgtaa
     4201 aatattttgt tcgccaaaag ccacgtcaac ggattcggtt tcttcggtgg aaaaaggaat
     4261 cgactggagt tcgactagaa ctgactctgt cactcgagta acggtaattt gcccatctat
     4321 tgggagcggc tcattctctg ccgggacgct ggtgtacatg cccatgagcg gaattcccgc
     4381 c
//
LOCUS       DAOQNJ010000177         4368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12589_length_4368_cov_14.532344, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000177 DAOQNJ010000000
VERSION     DAOQNJ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12589_length_4368_cov_14.532344"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLG52_06435"
     CDS             complement(<1..258)
                     /locus_tag="PLG52_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41117.1"
                     /translation="MALTTTQDFTTKTKDLIDSLKSVCANYGLGNDGNEFKIITQTFL
                     YKFMNDKFGYEVKRKNPKLAKAESWEKEVGGFTDSEYEMLLL"
     gene            complement(362..937)
                     /locus_tag="PLG52_06440"
     CDS             complement(362..937)
                     /locus_tag="PLG52_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41118.1"
                     /translation="MQNEPIEVTLKVTGVFELLGIPYVIGGSLASTLYGMVRTTQDSD
                     IVAEMRREHLQPFVSALQDEFYIDEEMIADSIEHQSSFNIIHRETMFKVDVFIPRPRP
                     FLRSQLARAQRQTFTFESELSAKFASPEDTILSKLEWYRMGGEVSDRQWRDILGVLKT
                     RAGELDLEYLKKWAGELKVGDLLKLALDEAG"
     gene            complement(927..1181)
                     /locus_tag="PLG52_06445"
     CDS             complement(927..1181)
                     /locus_tag="PLG52_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41119.1"
                     /translation="MKLLSADTHPKMEALQIQLWRQASPTRKMQMVAQLNASAHTLAL
                     MGLRSQHPQASEAELRRRLASLLLGDELARKVYGDLSHAK"
     gene            complement(1190..4267)
                     /locus_tag="PLG52_06450"
     CDS             complement(1190..4267)
                     /locus_tag="PLG52_06450"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNB41120.1"
                     /translation="MSFNENTRVKIPAILHLSRLGYEYISLSKSKHDEDTNIFLDVFA
                     ESLLRINPGLDTESVKRVYQDVSLALENEDLGQAFYEMLTATSGTKLIDFENFDNNSF
                     HVVTELNYKNGDDEFRPDVILLINGMPLAFVEVKKPNNHDGILAERERINVRFKNKKF
                     KKFVNISQLLVFSNNMEYDTDSIEPIQGVFYSTTSYSNAIFHCFREEEELDLSALLKP
                     EDDDLENFVLKDNNLAIIKHSPEFLTNKDPNTPTNRMLTSLFSRERLAMLLKYGIAYV
                     KEGSGLEKHVMRYPQFFATKAIERKLGEGEKKGIIWHTQGSGKTALAFYNVRYLTDYF
                     HRKGIVPKFYFIVDRIDLMEQAKREFSSRSLSVNTVSSKDALLKSFRKKQAIENASGK
                     PEITVVNIQKFKDDTDTVKASDYDINTQRVYFLDEVHRSYDPKGSFLANLVSSDRDAI
                     IIGLTGTPLITGDNISRKVFGGYIHKYYYNASIADGYTLKLIREGIETEYKIQLEQAL
                     KEVEILKGDADKKTIYGHKKFAEPMLDYIVRDFVNSRTKYQDETIGGMVVCDSSEQAR
                     MLFKIFVNKYNPSQKTVEPVDMSYRKISEPPAEYGKFQQDTGRSFTASLILHDVGSKD
                     ERKEQVEDFKAGKIDFLFVYNMLLTGFDAKRLKKLYIARLLRDHNLLQMLTRVNRPYK
                     NFKYGFVVDFADIRAQFDKTNKAYFDELQAELGNEMENYSNLFKSSEEIEAEFKDIKE
                     KLFHYDTNNAEVFSQQISQIQDRKTMTDIRKALENARNLYNLARLFGHFDLLEKIDLR
                     KFNQLLDETIAHIELLGLKENIESDVDTTNLLNVALENVIFAFHKVSEDELVIADQLK
                     GTLRKTREALSANFDPSDPEFVSLYDELKRLFDKKNLSEVTQDDMRKNIGALEKIFDK
                     VNELNRKNNLLKAKYENDAKYARVHKRIVERSGISKRESEIQESLAEVKKQTDERVLY
                     NNKLMDSEAFFTQMLMKNVIEAFDKIKVKLDPDSARFINALLVKEYMGEYQGI"
BASE COUNT          959 a         1143 c         1023 g         1243 t
ORIGIN      
        1 cagcaacagc atttcatatt cgctgtcagt gaatccacct acttcttttt cccagctttc
       61 ggcttttgcc agcttcgggt tcttgcgctt cacctcatag ccaaacttat cgttcatgaa
      121 cttatacaaa aatgtctgcg tgatgatctt gaactcgttg ccgtcattgc cgagtccata
      181 attggcgcac acgcttttca gcgagtcgat caggtctttg gttttggtgg tgaaatcttg
      241 tgtcgtggtt aatgccatgt gtcctcttca aatctgcccc cacccatctc ccccaaatac
      301 gacagaagta ccgtcgtatt tgggggagat gtccgaagga cagagggggt caggggtcgg
      361 tttaccctgc ttcgtccaaa gcgagcttca gcaaatcgcc aaccttgagt tcccccgccc
      421 attttttcaa atactccagg tcaagttccc ctgcgcgggt tttcagcacg cccaaaatat
      481 cgcgccactg acggtctgat acttcgccac ccatgcgata ccattcgagt ttggaaagga
      541 tggtgtcttc agggctggca aatttcgcgc tgagttctga ttcgaatgtg aatgtctgtc
      601 tttgagcgcg ggcaagttgt gatcgcagga acggtcgcgg gcgcgggatg aaaacatcca
      661 ccttgaacat ggtctcgcgg tgaatgatat tgaaactgga ttgatgctcg atggagtcgg
      721 cgatcatttc ttcgtcaata taaaattcgt cctgcaaagc cgaaacaaac ggctggagat
      781 gctcgcgccg catctctgcc acaatatcag agtcctgtgt tgtgcgcacc ataccgtaaa
      841 gcgtgctggc aagcgagccg ccgatcacgt atggaatgcc aagtagttca aagacacccg
      901 tcaccttaag cgtgacctca atgggctcat tttgcatggc tgaggtctcc ataaactttg
      961 cgcgccagtt catctcccaa taacaggctt gccagtctgc ggcgcagttc ggcttcactg
     1021 gcttgcggat gttgcgaccg cagacccatt aaagccagcg tatgcgccga ggcgttgagt
     1081 tgcgccacca tctgcatttt ccgcgtaggg ctggcctgcc gccataattg gatttgcaga
     1141 gcttccattt tggggtgtgt atcagcagat agaagtttca tcgctctgat tatatcccct
     1201 gatactcgcc catatattcc ttcaccagca gggcattgat aaaccttgct gagtcagggt
     1261 ctaacttcac cttgatttta tcaaacgcct cgatcacatt cttcatcagc atttgggtga
     1321 agaaggcttc cgaatccatc aacttgttgt tatacaacac ccgctcatcg gtctgcttct
     1381 tcacctccgc cagcgattcc tgaatctcac tctcgcgctt tgaaatgccg ctgcgctcca
     1441 caatgcgctt gtgaacgcgg gcatacttcg cgtcgttttc gtatttggct ttcagcaggt
     1501 tattcttgcg gttgagttcg ttcaccttgt caaaaatctt ctccaacgcg ccgatattct
     1561 ttcgcatgtc atcctgcgtt acttcgctga ggttcttctt gtcgaataag cgtttcaatt
     1621 catcgtatag cgaaacaaac tcagggtcgc tggggtcgaa gttggcgctc agggcttcgc
     1681 gggtttttcg cagcgttccc ttgagttggt cggcgatcac cagttcatct tctgaaacct
     1741 tgtggaaggc aaagatcaca ttttccaacg ccacgttcag caggttggtc gtatccacat
     1801 cgctttcgat gttttccttc aaacccagca attcaatgtg cgcgatggtc tcatccagca
     1861 gttgattgaa cttcctgaga tcgatcttct ccagcaggtc gaaatgaccg aacagccgcg
     1921 ccagattata taaattccgc gcattctcca gcgccttgcg gatgtcggtc atcgtcttgc
     1981 ggtcttggat ctggctgatc tgttgcgaga acacctcggc attgttcgtg tcgtagtgaa
     2041 agagtttttc cttgatgtct ttgaattccg cttcgatctc ttcactgctc ttgaacaagt
     2101 tggagtaatt ctccatctcg ttgccgagtt ccgcctgcag ctcgtcgaaa tacgccttgt
     2161 tggtcttgtc aaattgtgcg cggatgtcgg caaagtccac cacgaagcca tacttgaagt
     2221 ttttatacgg gcggttcacg cgcgtgagca tttgcagtag gttatggtcg cgcagcaggc
     2281 gggcaatgta tagcttcttc aagcgtttgg catcgaagcc cgtcagcagc atgttataca
     2341 caaacaggaa atcgatcttt cccgctttga aatcttccac ttgctctttg cgctcatcct
     2401 tcgagccaac gtcatgcagg atcaaagacg cagtaaaact cctgcccgtg tcctgctgga
     2461 actttccgta ttcggcgggc ggctcggaga tttttcggta gctcatgtcc acaggttcca
     2521 ccgtcttctg gctggggttg tacttgttga cgaagatctt gaacaacatc cgcgcctgtt
     2581 ccgaactgtc acacaccacc atcccgccga tggtttcatc ctgatatttg gtgcggctgt
     2641 tgacaaaatc ccgcacgatg tagtccagca tcggctcggc aaatttcttg tggccgtaga
     2701 tggtcttctt gtctgcgtcg cctttcagaa tttcaacctc tttgagcgcc tgctccagtt
     2761 ggatcttgta ctcggtctcg atcccttcac gaatcaactt caacgtgtac ccgtccgcaa
     2821 tggaggcgtt gtaatagtat ttgtgaatgt acccgccaaa caccttgcgc gaaatattat
     2881 cacccgtgat cagcggcgtc cccgtcaagc cgatgatgat cgcgtctctg tccgaactga
     2941 ccagattggc aaggaaactt ccctttggat cataactgcg gtgaacttca tccagaaaat
     3001 acacgcgctg ggtgttgatg tcataatcgc tggctttcac cgtgtccgtg tcatctttga
     3061 acttttggat gttgacgacg gttatctcag gcttgccgct ggcattttca atggcttgct
     3121 ttttcctgaa acttttcaac agtgcgtctt tggaactgac cgtattcacg ctcaaactgc
     3181 ggctggaaaa ctcgcgtttg gcctgctcca tcaaatcaat gcggtccacg atgaaataga
     3241 acttcggcac aatccccttg cggtggaagt agtctgtcag gtagcgcacg ttgtagaaag
     3301 ccaatgcagt ttttccgctg ccctgcgtat gccagataat gcccttcttt tcgccttcgc
     3361 ccaatttgcg ctcgatggct ttggtggcaa agaattgcgg atagcgcatc acgtgctttt
     3421 ccagtccgct gccttctttc acataagcaa tgccatactt aagcagcatc gcaagccgct
     3481 cacggctaaa gagcgaggtc aacatgcggt tggtgggcgt gttggggtct ttgttggtca
     3541 aaaattctgg cgagtgcttg atgatggcaa ggttgttatc tttgagcaca aaattttcaa
     3601 ggtcatcgtc ttcgggtttc aagagcgcgg agaggtcgag ttcttcctct tccctgaaac
     3661 aatgaaagat cgcgttcgag taggaggtcg tcgaatagaa tacaccctga atcggctcga
     3721 tggaatccgt atcatattcc atgttgttag agaaaaccag aagttgggag atattgacga
     3781 actttttgaa cttcttgttt ttgaaacgca cattgattcg ttcccgttcc gccagaatac
     3841 catcatggtt gtttggcttc ttcacttcca caaacgccaa aggcatacca ttgatcaaca
     3901 gaatcacgtc gggtcggaat tcatcatccc cgtttttgta gttcaattcc gtgaccacat
     3961 gaaaagagtt gttgtcaaaa ttctcaaaat caatcagctt tgtacctgag gtggcggtca
     4021 gcatttcata gaacgcctgc cccaaatcct cattctccaa cgccaacgaa acatcctgat
     4081 agacacgttt tactgactcc gtatctaggc cagggttgat acgcagtagc gactcggcga
     4141 aaacgtctag gaagatattg gtatcttcat cgtgctttga tttggataat gaaatatact
     4201 catatcccaa cctgctcaag tgtaaaatcg cggggatttt tactctggtg ttctcgttga
     4261 agctcatgtg cttgtccctt ttgtctctag aaacgtttaa ctttcgcagg gaattccccg
     4321 ctgaatttcc ccaagtatac cccacccaaa atccacgtcc acgggcgc
//
LOCUS       DAOQNJ010000178         4350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12667_length_4350_cov_12.010244, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000178 DAOQNJ010000000
VERSION     DAOQNJ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12667_length_4350_cov_12.010244"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="PLG52_06455"
     CDS             complement(<1..1617)
                     /locus_tag="PLG52_06455"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNB41121.1"
                     /translation="MMPNTFISYSRQCIGFVDDLAHKLQKQGFSIWTDYLSLVPGRPW
                     AEQIEKGLQEAELLLLVVSPESVSSRSVEIEWRHFLEHKKRIILLIFQAVPLPPELAG
                     LEWVDFRGAFAPAMKELLKRIEAPSPQRGLAPQSGFKAPFVVWVAAGLSVINAVYSLF
                     AFWTLLIPWVLVPLPARIFKREFNLSQVQTALWTQPIALFFTLGLFLELKLINIDVDF
                     DYSNYFSLYNMVLMSLFIQIYLIPFFSILLLIVLRLPGMQRWGRPEASAPKFANIYRP
                     NIVKPDPVHYFIDYAPEDKAIARELEKELTKVGHTPANGLASANEALVLLSRFKTDTE
                     ADPEKQVVYPILIQRVKLPEKLSKVQWIDFRKGVRNLEAIAKLLPQPAKLLTALGVRP
                     TSSGQAAMPNIVIALVDFLIIMGMVNLGSFFLYMLELTSLNLRLVAEYESFRLLWMIF
                     MQLLSTVASGWLIYFMVRSLSDRIGWFTKPLALSLGFIVILLLFFWQLILGSNINDLL
                     LHYGIVAETLFYALPFMFMFTGLCIVIVLVL"
     gene            complement(1636..3300)
                     /locus_tag="PLG52_06460"
     CDS             complement(1636..3300)
                     /locus_tag="PLG52_06460"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNB41122.1"
                     /translation="MTPNIFMSYSRRETGFVDDLTHRLEKAGFNVWLDYRRLIPGTPW
                     AGQIQKGLQEAEVVLLVVSKDSIASQYVELEWRHVLNEKNKRIILVIFEAVTLPDELK
                     KYEWVDFRGSYEAGIKELISQLNQPIQETQAPPESGFKAPFIVWVSILLSAIAGVYSL
                     LSFWSVLLPLILIPLAWQVYKRNYNFSRVQTALWMSPLLNLLGLLLFSELGYFNAEGE
                     NFAVYYFRINPLLGVLVNILQWLPYSAFVLIFLFRSASMQRWGKPEANMPKFANPYRP
                     KNPNPRPVKFFIDHAEQDSVIAKDISNKLIQYGHLQEADIKSAESVLVLISRFNTDTT
                     ADPEHQIVFPVLVQTAQPSEKLSHVQWIDYRKGLKNLDALAQLLPDPTLLLTALGTRP
                     TNGTQIVMPNIFSRVVNFLTFILLLNVSTFFIYILELWANGLGYIQQYEPAFIFSNLA
                     QTALAVLVACGLGFFIIKSVTERRGLFASIPPFFAGLFGLFLILMYQYTLADYMTTLF
                     EDYGETEIYGFITIPYLFLIFGGIVLLIMIFFNWKELRRWFPKKGK"
     gene            3431..>4350
                     /locus_tag="PLG52_06465"
     CDS             3431..>4350
                     /locus_tag="PLG52_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNB41123.1"
                     /translation="MTSTLPVLRARRERRLEKQKRNENRSRGAILSVGLILSLVFGGL
                     IILGAFAYSDVTRDLPSPQILRRLLNPPDGLLLQPTRVYDRTGLQLLATFAPSESPRR
                     YIPISEQNAQHVPAEMVKALIAVADPSFETHSGYSLEGFNNYDMHPTIAQKLVSALML
                     YDEPPSLKRAIRERLLAVQITSQFGRTQIMEWYLNSADFGNYAYGIDAAAQLYFGKPA
                     DELTLAESAILAATSETPALNPHDAPQVAIQRGRELLYVMSDLGLVTAEQVDPALAET
                     PTFTEAPQTPAEVAPAFLNLLLSQVDSQIPR"
BASE COUNT         1195 a         1024 c         1075 g         1056 t
ORIGIN      
        1 gagcacgagc acgatcacaa tgcacagccc tgtgaacatg aacatgaaag gcagagcata
       61 aaagagagtt tctgccacaa tgccatagtg aagtaaaagg tcattgatat ttgaaccaag
      121 aatgagttgc caaaagaaca gcaatagaat gacgatgaag cctagcgaca atgccaaggg
      181 tttagtaaac cacccgatgc ggtcactcaa cgaacggacc atgaaataga tcaaccagcc
      241 cgaagcgacg gtggagagca attgcataaa gatcatccac agcaggcgaa aggattcata
      301 ctctgccacc aagcgcaggt tgaggctggt cagctcgagc atgtaaagga agaaagagcc
      361 aaggtttacc atgcccatga tgatgagaaa gtctacaagg gcgatgacga tgttgggcat
      421 ggcggcttgt ccgctgctgg tggggcggac gcccaaagct gtgagtagtt tggcaggctg
      481 cggcagaagc ttggcgatgg cttcgagatt gcgcacccct ttgcggaagt cgatccactg
      541 caccttggag agtttctcgg gtagtttcac gcgttggatc aggatgggat acaccacctg
      601 tttttcaggg tccgcttcgg tgtccgtttt gaagcgcgag agcaacacca gcgcctcatt
      661 cgcggatgcg agtccattcg cgggggtgtg accaaccttg gtcaactcct tttccagctc
      721 gcgggcaatg gctttgtctt ctggggcgta atcaatgaag tagtggacgg ggtctggctt
      781 aacaatattc gggcgataga tattggcaaa ctttggggcg ctggcttcgg gtctgcccca
      841 gcgttgcatg ccgggtaagc gtaagacgat caacagcaag atggaaaaga aggggatgag
      901 atagatctgt atgaagagac tcatcaacac catgttatag agcgagaagt aattgctgta
      961 gtcaaaatct acatcgatgt tgatgagctt caattcgaga aacaagccca gtgtgaaaaa
     1021 caaagcaatg ggttgcgtcc acaacgcggt ttggacttgc gacaaattga actcgcgttt
     1081 gaagatgcgc gcgggcagag gaactaacac ccagggaatc agcagggtcc aaaaagcgaa
     1141 gagggaataa acggcattga tgacgctgag acccgccgcc acccaaacga cgaacggggc
     1201 tttgaaccca gactgcggag cgagtccacg ctgtgggctg ggcgcctcga tccgtttcag
     1261 gagttctttc atggctgggg caaaggcacc gcgaaagtcc acccattcca agccggctag
     1321 ctctggcggc aaaggcacgg cttgaaagat caataagatg atgcgctttt tatgctcgag
     1381 aaagtgccgc cattcgatct ccacgctgcg agaggaaaca gactctggcg agacgaccaa
     1441 gagcagcagc tcggcttctt gaagtccctt ttcgatctgt tctgcccatg gtcgccccgg
     1501 caccaggctg aggtaatctg tccagatgct gaagccttgt ttttgcaact tgtgggcaag
     1561 gtcatctaca aaaccaatgc attgccgtga ataactgata aaggtgttgg gcatcatagg
     1621 gtagaggtta aaaatttatt tccctttttt agggaaccaa cggcgtaact ctttccaatt
     1681 gaagaagatc ataatcagca aaacaattcc gccaaaaata agaaacaaat atggaatggt
     1741 aataaaacca taaatttctg tttcgccata gtcttcgaag agtgtggtca tgtaatctgc
     1801 cagggtgtat tgatacatga gaatgagaaa cagaccaaat agccctgcaa aaaacggcgg
     1861 gatggatgca aagagtccgc gcctttctgt taccgatttg attatgaaaa atccaagtcc
     1921 gcacgccaca agcacagcca atgccgtttg cgccaggttg ctaaagatga aagccggttc
     1981 atactgttgg atgtatccca acccatttgc ccatagctcc agaatataga taaaaaacgt
     2041 actgacattt agcaaaagaa taaaagttaa gaagttgaca actctactga agatgtttgg
     2101 catcacaatt tgtgtgccat tggtgggacg cgtgcccaat gcagttaaca atagggttgg
     2161 gtctggcaat aattgtgcca atgcgtctaa atttttcaac cctttgcgat aatcaatcca
     2221 ttgcacatgc gaaagttttt ctgaaggttg tgcggtctga accaaaacgg ggaaaacgat
     2281 ttgatgttcg gggtcagcgg tcgtgtcggt gttgaagcgc gagattaata ccaacactga
     2341 ctcagcagat ttgatgtccg cttcttgaag atgcccatac tggatcaact tgtttgatat
     2401 atccttcgcg ataacagaat cctgttctgc atgatcgata aagaatttaa cagggcgggg
     2461 attgggattt tttggacgat atggattggc aaatttcggc atgttcgctt cgggcttgcc
     2521 ccaacgttgc atacttgccg aacgaaaaag aaagatcaaa acgaaagccg aatagggcag
     2581 ccactgtaag atgtttacca atacccccaa taatgggttg atgcgaaaat aatatactgc
     2641 aaaattttcg ccttccgcat taaagtaacc aagttcacta aagagtagta agccaagcag
     2701 gtttaataaa ggtgacatcc acaaggcagt ttggacgcgt gagaagttat agttgcgttt
     2761 atagacttgc caggcgagtg gaatcagaat caatggaagc aatacgctcc aaaaggaaag
     2821 cagcgaataa acgccagcga tggcgctcaa aaggatgctt acccacacga tgaagggcgc
     2881 tttgaatccg ctttcaggcg gggcttgtgt ttcttgaata ggttgattga gttgtgaaat
     2941 taattctttg atgccagctt cataactacc tcgaaaatcc acccactcat attttttcaa
     3001 ttcatccggt aacgtcacgg cttcaaagat gacaagaata atgcgcttgt tcttttcgtt
     3061 taatacatgt cgccactcta attctacata ctgtgaagca attgaatctt tggaaacaac
     3121 caacaaaacc acttctgctt cttgcaagcc tttttgaatt tgcccagccc acggcgtgcc
     3181 tggaattaac cttcggtaat cgagccagac gttaaaaccg gctttctcca gcctatgggt
     3241 taggtcatct acaaaccctg tttcgcgcct tgaataactc ataaagatat ttggtgtcat
     3301 gaaagtctcc aataaattga gatgcgttgc aggcgaacat gcttaattgt ccagtaaacc
     3361 ttgtcattat aaaacaccaa actgacaatt cccgatttac caattttcaa aatcccaata
     3421 tactacctgt atgacttcta ctttaccggt cctccgtgcg cggcgtgagc gcaggcttga
     3481 aaaacaaaag cgcaacgaga accgttcgcg cggcgccatt ctcagcgtgg ggcttatcct
     3541 ctctctggtg tttggcgggc ttattatctt gggtgctttc gcttattcgg atgtcacccg
     3601 cgatttgcct tcgcctcaga ttttgcgacg cctgctcaac ccgcccgatg ggcttttgct
     3661 acagccaaca cgggtctacg accgaaccgg tttgcaattg ttagctacct ttgcgcccag
     3721 tgaatctccg cgccgttaca ttcccatcag cgaacaaaat gcccagcatg ttcccgccga
     3781 aatggtgaag gcactcattg cggttgcaga cccaagtttc gaaacgcatt cgggctattc
     3841 gctcgaaggg tttaataact atgatatgca tcccaccatt gcgcaaaagc tggtcagcgc
     3901 tttgatgttg tacgacgaac cgccatcttt gaagcgcgcc attcgcgagc gtttgcttgc
     3961 agtgcagatt acatctcagt tcgggcgcac acagatcatg gagtggtatc ttaacagcgc
     4021 tgattttggc aattatgcct atggtattga tgctgccgct caactttatt tcgggaaacc
     4081 tgccgatgaa ctaactctgg cagagtctgc gatcttggcg gcaaccagtg aaacgcctgc
     4141 cctgaatccg catgatgctc cgcaagttgc cattcaacgc ggacgtgagc ttttatatgt
     4201 aatgagcgac cttggactcg tcaccgccga gcaagtggac ccagccttgg ctgagacgcc
     4261 cacattcaca gaagcgcctc agactccagc cgaagtggct ccagcgtttc taaacttatt
     4321 gctctcccaa gtcgactctc aaattccgcg
//
LOCUS       DAOQNJ010000179         4345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12699_length_4345_cov_7.120280, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000179 DAOQNJ010000000
VERSION     DAOQNJ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12699_length_4345_cov_7.120280"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..930)
                     /locus_tag="PLG52_06470"
     CDS             complement(19..930)
                     /locus_tag="PLG52_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41124.1"
                     /translation="MMKRTIALTLSLLWLTACVTLTNPAPLIPTETVPTTPSVSAADN
                     TPTYVPLINNSALLYISGTTHIESKPQSWPNIDAFLNFLQNITAQGLKWSVGADIGWL
                     EGEPRAAELIQASEALGVQWDIHAHNPQERAKAAYLITQLGGHPTSVISGMLVSEFSS
                     IGPQTYQGLTWTPHVLWGGTNCPGHRPGCDDLAAGIWIPLSAEQYTTHDPNGTLIRVG
                     GGSHQLADGLVLAESIANRQYPYPVISFTLMVSPTSLTIVNSNDSLEQITAFANDMKQ
                     YPFVYWAYIEETAQAWTKAGKVPSRIE"
     gene            1101..1802
                     /locus_tag="PLG52_06475"
     CDS             1101..1802
                     /locus_tag="PLG52_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNB41125.1"
                     /translation="MKWNKDFIAGIKGAAPILIGVIPFAMITGVGAVSVGLSFWESMG
                     MSVFVFAGASQLVVFQLMSVGTPWIIMVLTAWVVNVRFTMYSATLAPYLQKLSTGRKA
                     LLAYMLSDQAFGVSMSRFISTDKEIDYRWYYFGAALIIWLAWQVSAVIGTLLGALVPA
                     SLGFDFAFPLSFMALMFGALRDRPTVVAALTGGFIAVLAKSLPYNTGLVLATFLGIIA
                     GYVAESLKLKEEAAL"
     gene            1799..2128
                     /locus_tag="PLG52_06480"
     CDS             1799..2128
                     /locus_tag="PLG52_06480"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNB41126.1"
                     /translation="MSALSIWLLFMAIGAGTFTLRFLFIYLFGKVEMPTWLSRALRFV
                     PAAALAALVFPALTHPAGHLDISLTNFRLLAGLGGALVAWRTRNVLLTILVGMALLWS
                     LQAAFGM"
     gene            complement(2160..4331)
                     /locus_tag="PLG52_06485"
     CDS             complement(2160..4331)
                     /locus_tag="PLG52_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB41127.1"
                     /translation="MTTATLPVQPQPSTLSKKILPGVTIGTLIVIVIMVASFGLHMLN
                     IDAIGNANEYYTAAVKSMLQSWSNFFFVAAEPGGSVTVDKPPLGLWIEAIFAYFLGVS
                     GFSVSLPNILAGVFGIGLLYSMVKKYMGELAGIIAALVMAITPVFVATNRNNTMDGML
                     VFTLLLAAWAFIHATETGKARWLMVGAFIVGLGFNIKMLQAFLPLPAFYALYFFGSKE
                     GWLRKIINLGIATVLLLVVSLSWAIAVDLTPAENRPYIGSSENNTVMGLIFGHNGISR
                     LENNRGGGNAPQTGTQPEQPTNQQTQPQGNPGQGQFQGPPPAALEACSGQTQGSACSF
                     TTQNGNTINGSCITPPNISELACAPQGMIPQNGQAPAGGPTGAPNGGPNGQNGNTPFG
                     NETGSPSALRFFTSPLSKQMSWLLPFALISVLIALFGSRIQLPIVSGVHKALLLWGGW
                     LLTCVVFFSMVSGIFHAYYAIMLAPALGAMVGVGFAQLYQWGKNSNWVLVGLIAAVVV
                     TLGFQIFAATQYNEFAWWMLGTGILLGLGILLMSVSRRAAYAVILSAALVIPAYWSVM
                     TVTNGSNNNLPTAYEGQNRNGQDGFLASPQQQNQGNRGNDRSVNTELLAYLEANTVGM
                     KYLMAVPSSQQGSQYVIETGRPVLYMGGFSGGDAVVTVDDLKELVANGELRYILYGGD
                     RGNQEITNWLSSSCAIVNDFNDLSQAQQGPQNQAMTLYSCR"
BASE COUNT         1058 a          961 c         1163 g         1163 t
ORIGIN      
        1 acgtgacctg tttttatttc actcaattcg actgggaacc tttccggctt tagtccaagc
       61 ttgagctgtc tcttcaatat atgcccaata tacaaaggga tactgcttca tatcgtttgc
      121 aaatgctgtg atttgttcta agctatcatt gctgttgact atggttagag aggtgggaga
      181 gaccattaat gtgaaactga taactggata tggatattgt ctatttgcga tagattcggc
      241 gagaactagc ccatcggcta attgatgaga gccaccgcca acgcggatga gtgtgccatt
      301 ggggtcgtga gtggtgtatt gctcagcact aagtggaatc cagatgcctg ccgctagatc
      361 atcacatcct gggcggtgac cgggacagtt cgtgccaccc cataacacgt gtggagtcca
      421 tgtgagacct tggtatgttt gaggtccgat gctactgaat tcactgacaa gcatcccaga
      481 tatgactgaa gtagggtgcc cgcctaattg agtgatgaga taagctgctt tggcacgctc
      541 ttgtgggtta tgggcatgga tatcccattg cacccccaat gcttcagagg cttggatcaa
      601 ttcagcagca cgcggttctc cttctagcca gcctatatct gctccaactg accatttcaa
      661 accttgtgct gtaatgtttt gaaggaaatt tagaaatgca tctatatttg gccatgattg
      721 cggcttggat tcaatgtgag tagtgccgga aatatagagt aatgcgctgt tattgataag
      781 cggcacgtat gtgggagtat tgtcggctgc agagacactt ggggttgttg gaacagtttc
      841 tgttggaata agaggcgcag gattcgtcag agttacacat gctgtgagcc ataacaaact
      901 aagagtgagg gcaatcgttc gtttcatcat attctccttt aaggtagatt ctatgatttt
      961 gaaaaattta gtaagagtga ctcgcatcat gcttgcatgg aattaatcgt gacagctatc
     1021 atagagttgt aaatagattg taaaaaaccg tagagttcag gggtagaatt gccccctaaa
     1081 aaatacagta tggataaatt atgaaatgga ataaagattt tatagctgga ataaaagggg
     1141 cagccccaat cttgattgga gtgattcctt ttgcgatgat cactggagtg ggagcggtga
     1201 gtgttggttt gtcattttgg gagtcgatgg ggatgtctgt gtttgtgttt gcgggggctt
     1261 ctcagttggt ggtgtttcaa ctgatgagtg taggcacccc atggattatt atggttttaa
     1321 cggcgtgggt ggtaaatgta cgttttacga tgtacagtgc tacattggcg ccatatttac
     1381 aaaaactttc tacgggacgt aaagcattgt tggcatatat gctctcagac caggcatttg
     1441 gggtcagtat gagccgcttt atttccaccg ataaagaaat agactatcgg tggtattact
     1501 ttggcgctgc gctgatcatc tggttggcat ggcaagttag cgctgtgatt gggacattgt
     1561 taggtgctct tgtacccgcc agcctcggct ttgactttgc ctttccgctc agtttcatgg
     1621 cgttgatgtt cggagctttg cgagaccgcc ctacagtagt agcagcattg accggcggtt
     1681 ttatcgccgt gttggcgaaa agcttgccgt ataacactgg gttggtattg gcaacctttc
     1741 ttgggattat tgccgggtat gttgctgaaa gtctaaagtt aaaagaggag gcagccctat
     1801 gagcgcatta tcgatttggc ttctctttat ggcgattgga gcgggcacat ttacgcttcg
     1861 atttttgttt atttatctct ttggcaaagt tgagatgccc acttggttga gccgggcgtt
     1921 aaggttcgtc cctgcggcag cgttggcggc gttggtcttc ccagcgttga ctcaccctgc
     1981 ggggcacctt gatatttcgc tcacgaattt ccgtctcttg gcagggcttg gtggggcatt
     2041 ggtggcgtgg cgcacaagaa atgttctgct taccattttg gtgggcatgg ctttgttgtg
     2101 gtctcttcaa gcagcatttg ggatgtaaaa aagccccgag attatctcgg ggctttttat
     2161 tatctgcagg agtataaggt catggcttga ttttgtggac cctgctgcgc ttgagataaa
     2221 tcattgaaat cattcacaat cgcacatgag ctacttagcc aattggtaat ttcttggttg
     2281 cctctatcgc cgccgtagag aatgtaacgc aactctccat tggcaactaa ttctttcaga
     2341 tcatccactg ttacaacggc atcaccgccg ctgaaaccac ccatgtataa gaccgggcgc
     2401 ccggtttcga ttacatattg tgaaccctgt tgagaagatg gaaccgccat aagatacttc
     2461 atgcctaccg tgtttgcttc caagtatgcc aacagttccg tattcacact gcggtcatta
     2521 ccgcggttgc cttgattttg ctgttgtggg ctggcgagaa agccatcttg cccgttgcgg
     2581 ttctgccctt cataggcggt gggtaggttg ttgttcgacc cattggtgac ggtcataaca
     2641 ctccaataag cggggatgac caaagctgca ctgaggatga cggcatacgc cgcacgcctc
     2701 gaaacggaca tgagcaggat tcccaaccca agcaggatgc ccgttccaag catccaccat
     2761 gcaaattcgt tgtattgcgt ggcggcaaag atctggaaac caagtgtgac aacaaccgct
     2821 gcgatcagac caaccaagac ccaattcgag ttcttgcccc attgataaag ctgggcaaag
     2881 ccaacaccaa ccatggctcc taatgccggg gcaagcatga tggcatagta agcgtggaag
     2941 atgccagaaa ccatgctaaa gaagactaca caggtgagta accatccacc ccagaggagc
     3001 agggctttat gtacccccga tacgataggg agttgaatgc gtgagccgaa taaggcaatc
     3061 agaacactga tcaatgcgaa tggcagcaac cacgacattt gtttggaaag aggtgaggtg
     3121 aagaagcgca aagcacttgg agagccggtc tcattcccaa atggggtgtt gccgttctgc
     3181 ccattcggac cgccatttgg cgccccagtg ggtccacctg caggagcttg cccgttttga
     3241 gggatcattc cttgaggagc acaagctagt tctgaaatat tgggcggggt aatacaagaa
     3301 ccattgatgg tattgccatt ttgcgtggtg aatgaacaag cagacccttg cgtttgacca
     3361 gaacaggctt ccagcgcggc aggaggtgga ccttggaact gcccttgacc gggattccct
     3421 tgcggctgag tctgttgatt ggtgggttgc tcaggctgag tgcccgtttg aggcgcattt
     3481 ccgcctccac ggttgttctc aaggcgtgaa atgccgttat gcccgaagat caatcccatc
     3541 accgtgttat tttcgctgga gccgatgtag gggcgattct cggcgggggt gaggtcaacg
     3601 gcgattgccc acgaaagtga gaccactagt aagaggacgg tagcgatgcc gaggttgatg
     3661 atttttctga gccagccttc ttttgagcca aagaagtaca aggcatagaa agccggcaaa
     3721 ggtaaaaagg cttgcagcat tttgatgttg aaacccaagc cgacgatgaa agcgcctacc
     3781 atgagccagc gtgctttacc ggtttcagtg gcgtggatga acgcccacgc agcaagcaag
     3841 agtgtgaaga ccaacatacc gtccatagtg ttgttgcggt tggtggcaac aaagaccggg
     3901 gtgatcgcca tgacgagtgc cgcaataatc cctgctaact cgcccatgta tttcttgacc
     3961 atgctgtaga gcaacccgat accgaataca cccgcaagga tattcggcaa tgaaacacta
     4021 aacccgctca cgcccagaaa atacgcgaag atggcttcga tccataaccc tagcggaggt
     4081 ttatctactg tgacagaccc gcccggttcg gcggcaacga agaaaaagtt gctccacgat
     4141 tgcagcatgg atttgactgc ggcagtgtag tactcattgg cattgccgat ggcgtcaata
     4201 ttgagcatgt gcaacccaaa ggatgcaacc atgatcacaa tcacgatcag tgtgccaatg
     4261 gttacaccgg gcaaaatctt tttgctcaag gttgagggtt gaggttgaac aggtaaagtt
     4321 gctgtagtca tttagtactc cttgt
//
LOCUS       DAOQNJ010000180         4336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12749_length_4336_cov_8.410418, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000180 DAOQNJ010000000
VERSION     DAOQNJ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12749_length_4336_cov_8.410418"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PLG52_06490"
     CDS             complement(<1..562)
                     /locus_tag="PLG52_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41128.1"
                     /translation="MKKIFQVISVIFLTLALLANDSRSSQAAPIFQLEADIVTGTYGP
                     SDSNPSYMTEFNGKLYFNANADSTYDYELYVYDGVNPPSLVADIYPGKIGSYPMALAV
                     FNNALYFGAYGSESQGTELWKYDGVNPPSLVADICVGICHSTPDHLRVFDNALYFSAD
                     GGDGAGMELWKYDGVNPPARVVDLYSG"
     gene            complement(616..810)
                     /locus_tag="PLG52_06495"
     CDS             complement(616..810)
                     /locus_tag="PLG52_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41129.1"
                     /translation="MQTKGNSLRRYHSYLLRIWCEDDMLAWRFQLEDPNTHEVIGFQN
                     MEKLLCFLNEQVFHSDGSIT"
     gene            961..3948
                     /locus_tag="PLG52_06500"
     CDS             961..3948
                     /locus_tag="PLG52_06500"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB41130.1"
                     /translation="MSQLEINLLGGALIEREKKQVGGFPSRAAEALFVYLACVQKPVT
                     REKLAELLWADRSPTQSLTNLRTILTSLRRELDDYLIVTRDTLAFNPEADAFIDIFEF
                     ERGLKGLGFPDRASTLTPSEQETLRLTLDLYRGDFLDGFHLRDGLGFEEWSILERERF
                     KRLARDGFRAYTHACLASGLYAQGLTAASSWLRLDPFDEEACRAQMWLFMRTSQRTAA
                     LQTYQALKKKLSDELGVSPASATTDLYRRFQNIEVPPAIHLPAYVTEFVGREEEIAEM
                     EEKLSMSGTRLMTVTGTGGMGKTRLSVEAALRLSSKKPGAFLHGVHFIPLEMSSSADE
                     MIVRIAEGIGFSFHGSDLPSVQLLGHLREQEMLLLFDNFEQLLGEGSDALTFIVEVLR
                     NAAGVKLIVTSRERLNLYEETVFDLAGLSDAVTLFAHCAQRVRRDFALTLDTQPHVSH
                     ICQLVDGSPLAIELASAWARHLTPAQIVSQIQSDLDFLQSPFSNAPAGHRSLRAVFER
                     SWSLLQPQEQNAFALLSIFRGGFTPEAADALLGEPSQSLLVELSDQSLLQRNADGRYD
                     IHPLLKQYTFEKLSSQPEWLKNASARHTSFYLTFLTGQGDGESPQERAAIRPELDNIR
                     LAWESAAREDRIVELEQTAGTLHGFFSVQSWFEEGTALFQHILTLIEASSHANADGLR
                     CDLLGRNARMHIQIGQLEKARSDLKQAQTYLEHLDDPSRRARVLDSLAINSYYAGDYP
                     QATALALESLALSESMNNADGAAFSLNFLGSCAKAAGDFAKSHEYFERSVSAYRTMRD
                     EIGAAMVMNNLGNLLQAQGDLQGAQTYYVDASVILKEQDHAHGAATTLTNAGKLALKR
                     GQLDEARTLLEEGLSMKRRINDKRGEAVALAGLGDIALAANALEDSQTHLRQALELAA
                     HTGDTQLALDILAAIASLTARQNRPDLAGRLLAYLLDHPGLAEETRQRVLELKEKIPP
                     ADNVLWKGQDLLEDVLPKVLEDL"
     gene            complement(4001..>4336)
                     /locus_tag="PLG52_06505"
     CDS             complement(4001..>4336)
                     /locus_tag="PLG52_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNB41131.1"
                     /translation="TSGHWAEDWIEALKNDGITSGCGTGIYCPEDAVTRAQMAVFLLK
                     AKYGSGYVPMNSLPTFNDTAGHWAEDWIEQLAVEGITSGCAAGLYCPDNSVTRDQMAV
                     FLVKAFNLP"
BASE COUNT         1037 a         1100 c         1117 g         1082 t
ORIGIN      
        1 ctccgctgta aagatcaact acgcgtgcgg gcgggttgac tccatcgtat ttccacagtt
       61 ccatccctgc gccatcaccg ccatctgcgc tgaaatagag ggcattatca aatactctca
      121 gatggtctgg agttgaatgg cagattccca cgcagatatc cgcaacaagg ctgggcggat
      181 taacgccgtc gtacttccat aattcagtgc cctggctttc gcttccatag gcgccaaagt
      241 aaagcgcatt gttgaaaaca gccaatgcca tggggtatga acctattttg ccggggtaaa
      301 tatccgccac cagactggga gggttaacgc cgtcgtacac ataaagttca taatcatatg
      361 tgctatcagc attggcgtta aagtacaatt tcccattgaa ctccgtcatg taggatgggt
      421 ttgagtctga agggccatag gttcctgtga cgatatccgc ctcaagttga aagattgggg
      481 cagcctgact tgacctgcta tcatttgcaa gtaaagccag tgtcagaaaa attactgaaa
      541 taacttgaaa tatctttttc ataaaacttt ctccttttta tttaaaaatt gccggtaata
      601 gcagatctca accgattaag taatgcttcc atccgaatgg aaaacttgtt cattcaaaaa
      661 acacaggagt ttctccatgt tctgaaagcc aatgacttca tgcgtattgg ggtcttctaa
      721 ttgaaaacgc caggccaaca tatcatcttc gcaccagatg cgtaggagat aggagtgata
      781 gcggcgcaaa gaatttcctt ttgtctgcat gctataattc aaacgttgtt tggttgattt
      841 ttgttttttc attgtgatga actgtcgctg aagtagattg tgtatgcctt cacagaatct
      901 gaaaattaaa taacgacttc aataactata tttaccaaac gcttagaaaa cgctcagaat
      961 atgtcgcaac tcgaaattaa tctcctgggt ggggcgctca ttgaaagaga aaagaaacaa
     1021 gtgggtggat ttccttcacg cgccgcagaa gccctttttg tttacctcgc ctgcgttcag
     1081 aagcccgtca cccgcgaaaa actcgccgaa ctactttggg cagaccgttc tcccacgcaa
     1141 tcgctcacaa acctgcgcac cattctcacc tccctgcgcc gcgaactgga cgattacctc
     1201 atcgtcaccc gcgacaccct cgccttcaac cctgaagccg acgccttcat tgatatcttt
     1261 gaatttgaac gcggactcaa aggattggga ttccctgacc gcgcctccac tctaacccca
     1321 tcagaacagg aaaccctgcg cctcaccctc gacctatacc ggggtgactt tctcgacggc
     1381 tttcacctac gggacggctt gggctttgaa gaatggagca tcctcgaacg cgagcgtttt
     1441 aagcgattag cccgcgatgg attccgcgct tatacccatg cctgcctcgc aagcggactc
     1501 tacgcccagg gtttaaccgc cgcctcaagc tggctacgcc tcgatccctt cgacgaagaa
     1561 gcctgccgcg cccaaatgtg gctgttcatg cgtaccagtc agcggacggc tgccctgcaa
     1621 acctatcaag ccctcaagaa aaagttgagc gatgaattgg gcgtttcacc tgcctcggca
     1681 accacagact tgtatcgtcg cttccaaaac atcgaggttc cgcctgcgat tcatttgcct
     1741 gcttacgtta cggaatttgt agggcgtgag gaagagatcg cggagatgga ggaaaaactg
     1801 tccatgtcgg gaacccgcct gatgacagta acaggcacgg gtggcatggg caagacccgg
     1861 ttaagtgttg aggcggctct tcgtctttca tccaaaaaac cgggcgcatt cctgcatggc
     1921 gttcacttca tcccgttgga aatgtcatct tctgctgatg aaatgatcgt gcgcattgcg
     1981 gagggaatcg gcttttcgtt tcacggctct gatttaccaa gcgtgcaatt gctggggcat
     2041 ttgcgtgaac aagagatgct gttgctgttc gataattttg agcaactgtt gggcgaaggc
     2101 agtgatgcgt tgacgtttat tgtagaggtg ttgcgaaatg cggcgggtgt gaagttgatc
     2161 gttacatcgc gggaacgtct gaacttatat gaagaaaccg tgtttgatct tgccgggttg
     2221 tcagatgccg tgactttgtt tgcgcattgt gcgcagcgcg tgcggcgtga ttttgcgttg
     2281 acgctggata cccagccgca tgtttcgcac atttgccagt tggtggatgg atcgccgctt
     2341 gcgattgagc tggcttcggc gtgggcgcgg catctcacgc ccgcgcagat cgtttcgcag
     2401 attcaaagcg accttgattt tctacaaagc ccattttcca atgctccggc tgggcatcgc
     2461 agtttgcgtg cggtcttcga gcgttcgtgg tccttgttgc agccgcagga acaaaacgcg
     2521 tttgcactgc tctcgatctt tcgcggcggg tttacaccgg aagccgccga tgccttgctg
     2581 ggtgagcctt cgcaatcttt gctggttgaa ttgagcgatc aatcattgtt acaacgcaat
     2641 gccgatggtc ggtacgatat tcatccgctc ttgaagcaat atacatttga gaagttatct
     2701 tcccaaccgg aatggctgaa aaacgcctcg gcgcggcaca catcgtttta cctgaccttc
     2761 ctaactggac aaggtgatgg cgagtcgccg caggaacgcg ccgccattcg acctgagttg
     2821 gataacatcc gtctcgcctg ggagtctgcg gcacgtgaag accgcatcgt tgagcttgag
     2881 caaaccgccg gaaccctgca cggatttttt agtgtgcaaa gctggttcga agaaggcacg
     2941 gcgctctttc aacatatttt gactttgatc gaagcgtcgt ctcatgccaa tgcagatggt
     3001 ttgcgttgtg acctgcttgg acgcaacgcc cgaatgcata ttcagatcgg tcaattggag
     3061 aaagcccgtt ctgatctgaa acaagcccag acctacctcg aacatttgga tgatccctcc
     3121 cgccgtgcgc gtgtgttgga ttcacttgcc atcaatagct attatgcggg cgattacccg
     3181 caagccacgg cgttggcatt ggaaagcctg gcgctttctg aatccatgaa caatgcagat
     3241 ggtgccgcct tctcgttaaa cttcctcggc agttgtgcca aagcggcggg cgattttgca
     3301 aagtcacatg aatactttga gcgttctgta tccgcctatc gcaccatgcg ggatgagatc
     3361 ggcgcggcaa tggtgatgaa taatttgggg aatctcttgc aggcgcaagg tgatctgcaa
     3421 ggagcgcaaa cctattatgt ggatgccagt gtcatcttaa aagaacaaga ccatgcacat
     3481 ggcgcggcga cgacgttaac caatgcgggt aaactcgcgc tcaaacgcgg tcaactggat
     3541 gaagcgcgga cattgctcga agaaggcttg tccatgaagc gccgtatcaa cgacaaacgc
     3601 ggcgaagcgg tggcattggc aggcttgggc gatattgcgc ttgcggcgaa tgctttggag
     3661 gatagccaaa ctcacctgcg ccaggcattg gaattggcgg ctcacaccgg cgacacgcaa
     3721 ctggctctcg atattctggc ggcaattgcc agcctgacag ccagacaaaa ccgtcccgat
     3781 cttgccggaa gattgcttgc ctatttactc gatcatcccg gactggcaga agagacccgt
     3841 caacgggtgt tggagttgaa agagaaaatt ccacctgctg acaatgtcct ttggaaggga
     3901 caggatttgt tggaagatgt attacccaaa gtgttggagg atttataaaa aaggcaactc
     3961 gcaagagttg ccttttttat aaaacatggc ttgagaaatc ttacggcaaa ttgaacgcct
     4021 taacgaggaa gacagccatc tggtcgcggg tgacggagtt atcggggcaa tatagcccag
     4081 cggcacatcc actcgtaatc ccttcgacgg ctaattgctc aatccagtct tcagcccagt
     4141 gaccggctgt gtcgttaaag gttggtaatg aattcatggg aacatagcct gaaccgtatt
     4201 ttgccttgag caagaataca gccatttgtg cacgagtgac ggcgtcttcg gggcaataga
     4261 tgcctgtgcc gcagccggag gtgatgccgt cattcttgag ggcctcgatc caatcctcgg
     4321 cccagtgacc ggacgt
//
LOCUS       DAOQNJ010000181         4326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12794_length_4326_cov_11.720674, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000181 DAOQNJ010000000
VERSION     DAOQNJ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12794_length_4326_cov_11.720674"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PLG52_06510"
     CDS             <1..1064
                     /locus_tag="PLG52_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNB41132.1"
                     /translation="PAISSIENMPHLLIAGTTGSGKSVCVNSILTCMLLYNTPDDLRL
                     VLVDPKRVELTGYNGVPHLLGPVVVEMDRVIGALQWITREMDKRYHMFAQVGSRNITD
                     YNAKMKLKGEKKLPYLVIVIDELADLMMIAPDETEKTITRLAQLARATGIHMILATQR
                     PSVDVVTGLIKANFPARIAFAVASNTDSRVILDQPGAEKLLGRGDMLYQAPDAPAAAR
                     LQGVFVSDNEIHRLVEYWQEQAGTGKPQPSDADVPANTIPENVPLKQVPLWEENPKVS
                     GDPLFDEAVAIVRKEGKGSVSMLQRRLRIGYTRASRIMDTMEDKKIVGPPEGATQMRQ
                     VLDYGDVQPPQNELPKEEE"
     gene            1219..1617
                     /locus_tag="PLG52_06515"
     CDS             1219..1617
                     /locus_tag="PLG52_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNB41133.1"
                     /translation="MTEKKIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQT
                     PEMIAEAALQEDVDVVGLSVLSGAHMALTPRVMELLRTNGQSHVKVFLGGIIPDEDLH
                     RLKEMGVTGVYGPGANTEDIIHDIKTAVAG"
     gene            1731..2735
                     /gene="meaB"
                     /locus_tag="PLG52_06520"
     CDS             1731..2735
                     /gene="meaB"
                     /locus_tag="PLG52_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNB41134.1"
                     /translation="MTSSQAILNGDRLALARMLTQVENNSPEGPIVLMELFPHTGKAH
                     LIGVTGAPGTGKSSLVNQLALHYRKKEDKRVAIVAIDPSSPFTGGAVLGDRVRMRDLS
                     GDPNVFIRSMASRGSLGGLAQATANVVQVFDAAGFDVVIVETVGAGQSEVDIARLAHT
                     TLVVEAPGLGDDIQAIKAGILEIADILVVNKGDRPGVENTEKALKTMLELAHPTERVF
                     KHHGTSMRVSAPKTTANAKMWIPPIQRTISTEGIGIAELAEAIARHVEHLHLSGDWAT
                     RERTRLEVELEALIREGLMTKFREQLTAEAYEKVIESVVERNVSPWEAAKGLLKGYSK
                     "
     gene            2732..3286
                     /locus_tag="PLG52_06525"
     CDS             2732..3286
                     /locus_tag="PLG52_06525"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNB41135.1"
                     /translation="MSIVYGKRIRLRAVEREDVQKFHEWINDPEVTLNLAMYLPMSMQ
                     DEMNWFEGLSKRDPHEKPFAIEIRKGKAWKLIGNCGVFGIEFTNRNGELGIMIGDKSE
                     WNKGYGAEAMSLLQRHCFETLNLNRVYLRVYEENVPAVRAYEKAGFVLEGRQRQAVYK
                     KGKYEDVLFMSVLRSEWDTRMKEK"
     gene            3292..4281
                     /locus_tag="PLG52_06530"
     CDS             3292..4281
                     /locus_tag="PLG52_06530"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNB41136.1"
                     /translation="MHHVHHDLKVYGGIKLFGGTGSPELSQKIAEYLDLPLSGREVIE
                     FPNENLFVKLNGSVRGQDVYVIQTTSSPVHRNLMELLIMIQTLKLDSAARITAVVPYL
                     CYGRSDKKDQPRVPITARLVADMLEAAGADRYMTLDPHAGQIQGFFSVPGDVLTASHL
                     LIHHINQTLRADMKDPVVVSVDLGFAKKGRNYAADMDMPIAFIEKRRHGNDAKAEALT
                     LIGEVKDRDVIIVDDEVDTGGSIAQAVNVVMQNGARDVYLAFIHPILSANGAARLASL
                     PIKQIITTNSVPLSPEKLKILEGKITVLSIAPLLGEVIRRAHEGRSVGEMFNE"
BASE COUNT         1109 a         1011 c         1154 g         1052 t
ORIGIN      
        1 tgcctgccat ctccagcatt gaaaacatgc cgcacttgtt gatcgcgggt acgacgggct
       61 ctggtaagtc tgtgtgtgtc aactcgattc tgacttgtat gcttttatac aacacccccg
      121 atgacttgcg ccttgtgctc gtagacccga agcgcgtgga gttgaccggg tataacggcg
      181 ttccgcatct gcttgggccc gtagtcgttg aaatggatcg tgtgatcggt gctttgcaat
      241 ggatcacccg cgaaatggac aagcgctatc atatgttcgc gcaggtcggt tcacgtaaca
      301 tcactgatta caacgccaag atgaagctga agggtgaaaa gaagttaccc tatctcgtca
      361 tcgtcatcga cgaattagca gacttgatga tgatcgcacc ggatgaaacc gaaaagacca
      421 tcacccgctt ggcacagttg gcacgtgcta ccggcattca tatgatcctc gctacgcaac
      481 gcccttctgt ggatgtggtg acgggtctca tcaaagccaa cttcccggcg cgtattgctt
      541 ttgctgtggc atccaacaca gacagccgcg ttatcctcga ccaacccggc gcagaaaaat
      601 tgctcgggcg cggtgacatg ctctaccaag caccagatgc ccccgctgcc gcccgtttgc
      661 aaggtgtgtt cgtctctgat aatgaaattc accgcttggt ggaatattgg caagaacaag
      721 ccggaacagg caaaccacaa ccatctgatg cggatgtgcc agccaatacc attcctgaaa
      781 atgtgccgct caaacaagtg ccgttatggg aagagaatcc caaagtgagc ggtgatccat
      841 tgttcgatga agctgtggcg attgtccgca aagaaggcaa aggctccgtg tctatgcttc
      901 aacgccgatt gcgcattggt tacactcgtg cctctcgcat catggacacg atggaagaca
      961 agaagattgt gggtccgcca gagggtgcca cccaaatgcg tcaggtgctg gattatgggg
     1021 atgtgcaacc accacaaaat gagctaccca aagaggaaga ataaaaataa aaggattgcc
     1081 acatggcaat ccttttattt tgtcgaattt attgtctgac aaaaaagatt ttatccaaca
     1141 tttttcctta catttttatt gaatctgtcg attgtgatgc atagtataat cacttcaacg
     1201 attcttatcg gaggataaat gaccgaaaag aaaattagag tgttagttgc aaaacctggt
     1261 ctcgacggac atgatcgcgg agcgaaagtt atcgcccgtg cgcttcgcga tgccgggatg
     1321 gaagtcatct acaccggctt gcgccaaacc cctgaaatga tcgcagaagc ggctcttcag
     1381 gaagatgtag atgttgttgg gttgtctgtg ctttcaggtg cacacatggc attgactccg
     1441 cgcgtgatgg aattgctgcg caccaatggt cagtcgcatg tcaaagtttt ccttgggggc
     1501 attatccctg atgaagactt gcaccgtttg aaagaaatgg gtgtcaccgg agtttatggt
     1561 cccggcgcga acaccgaaga catcattcac gatataaaaa cagcagtagc aggataaaag
     1621 catctcaaca cagagccctc tgctctgtgt tttgttctta ataacaaaca ggggcgacct
     1681 gtctatttaa agacaaggtc ttttctgcaa gaatgaatta caggaaatta atgacttctt
     1741 cccaagccat tctcaacggt gatcgccttg cccttgcgcg aatgttgacc caagtggaaa
     1801 acaattcgcc agagggtcca atcgtgttaa tggaactttt tccgcacacg ggcaaagcac
     1861 acctcatagg tgtcacaggc gcaccgggca cgggcaaatc gtcgctggtg aatcaactgg
     1921 cgctgcacta ccgcaaaaag gaagataaac gagttgcgat cgttgccatt gatccgtcca
     1981 gtccgttcac gggcggcgca gtgttgggag accgagttcg catgcgcgac ctttctggcg
     2041 acccgaatgt cttcattcgc tcgatggctt cgcgcggctc gctaggcgga ttagcacagg
     2101 caaccgcaaa tgttgtgcag gtctttgacg cggcggggtt tgacgtggtc attgtggaaa
     2161 ccgtgggcgc cgggcaaagt gaagtggata ttgcccgcct cgcccacacg acgttagttg
     2221 ttgaagctcc cgggcttggg gacgacatcc aagccattaa agccggtatc cttgagatcg
     2281 cagatattct cgtggtcaat aaaggcgata gacccggcgt agagaatacc gagaaagctt
     2341 tgaagaccat gcttgaattg gcgcacccaa ccgaacgagt tttcaagcat catggcacgt
     2401 ccatgcgagt ctctgcgccg aagacgactg caaacgccaa gatgtggatt ccgcccattc
     2461 aacggacgat ctccactgaa gggatcggta ttgcagaatt ggctgaggca attgctcggc
     2521 acgtggagca cctccacttg agtggcgatt gggcaactcg tgagcgaacc cggctcgagg
     2581 tcgagttaga ggcgttgatc cgcgagggat tgatgaccaa gtttcgagag cagttaacgg
     2641 ctgaggcgta tgaaaaggtt atcgagtcag ttgtcgaaag aaatgtttcg ccttgggaag
     2701 cggcaaaagg cttgttgaaa ggatattcca aatgagcatc gtatatggaa aacgaattcg
     2761 tttgcgcgcg gtggaacgtg aagatgtgca aaagtttcat gagtggatca atgacccaga
     2821 agtgacgctc aacctggcaa tgtacctacc catgtcgatg caggatgaaa tgaattggtt
     2881 cgagggcttg tcgaaacgtg acccgcatga gaaacctttt gccattgaaa ttcgcaaagg
     2941 caaggcttgg aagttgatcg ggaactgcgg tgtgtttggg attgagttta cgaatcgcaa
     3001 tggcgagctt ggaatcatga tcggcgataa atcggaatgg aataaaggct acggcgccga
     3061 agctatgagt ttgttacagc gtcattgttt tgagaccttg aacttaaacc gtgtctattt
     3121 acgggtatac gaagaaaatg tacctgccgt tcgtgcctat gaaaaagccg gttttgtttt
     3181 agaaggaaga cagcgccaag ctgtttataa aaaaggaaaa tacgaggatg ttctcttcat
     3241 gagcgtgttg cgctcggaat gggacacacg aatgaaggag aagtaatcca catgcatcat
     3301 gttcatcacg atttgaaagt gtacgggggg attaagttat tcggtgggac gggttcgccc
     3361 gaattatcgc aaaagatcgc agagtacctc gaccttccat taagtggacg cgaggtaatt
     3421 gagttcccca atgagaactt gttcgtcaaa ttgaatggca gtgtgcgtgg gcaagatgtg
     3481 tacgtcattc agacgacgtc ttctcctgtt catcgcaact tgatggaatt gctcatcatg
     3541 atccagactc tgaagctgga ttcagcagcg cgcattacag cggttgtgcc ttatctttgt
     3601 tatgggcgtt ctgataaaaa agatcagccg cgcgtgccga tcactgcccg tttggtggca
     3661 gatatgctcg aagccgctgg tgcagatcgc tacatgacgc tcgacccaca tgccgggcaa
     3721 attcagggtt tcttctctgt gccaggtgat gtgctgaccg cttcacactt gttaatccac
     3781 catatcaatc agactttgcg tgcggatatg aaagacccgg tcgtggtctc tgtcgatttg
     3841 ggttttgcca agaaggggcg taactacgcc gcagatatgg acatgcccat cgcttttatt
     3901 gaaaagcgcc gccatggcaa tgatgccaaa gccgaagcac tgaccttgat cggtgaagtg
     3961 aaagaccgcg atgtcattat tgtggatgat gaagtggaca ctggcggttc gattgcacag
     4021 gctgtgaatg tggttatgca aaatggcgca cgcgatgtgt atttggcgtt cattcacccc
     4081 atcctttcgg caaatggtgc agcgcgcttg gcgtctctgc ctattaaaca aatcattacc
     4141 acaaactcgg ttccgctttc ccctgagaaa ttaaaaatcc tcgaaggcaa gattactgtc
     4201 ctctcgattg cccccctgtt gggagaagta atccgccgcg cacacgaagg gcgctcggtt
     4261 ggagagatgt ttaacgaata gaagaacagc tttcagtagt cagctcgtag ctaatttctg
     4321 aaagct
//
LOCUS       DAOQNJ010000182         4294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_12954_length_4294_cov_7.998113, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000182 DAOQNJ010000000
VERSION     DAOQNJ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12954_length_4294_cov_7.998113"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLG52_06535"
     CDS             complement(<1..661)
                     /locus_tag="PLG52_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNB41137.1"
                     /translation="MTIHFGTDGWRAVISDTFTFSNLRMVSQAIADAVASEHWDKSEN
                     VDEKKIVIGYDTRFLSDRFAGEVGRVLAANGFSVLLAQSDSPTPAISYAVKNNNAIAG
                     VMITASHNAPRYNGVKLKGAFGGSALPEQCRRVEVYINDNEQQARGPNLMDFKKARDA
                     GLIQRFNPLPAYFDHLRKLINTDIIADNPQRFVVDAMYGSGRGVIKSFLQGTGCEIHE
                     IR"
     gene            complement(734..>1123)
                     /locus_tag="PLG52_06540"
     CDS             complement(734..>1123)
                     /locus_tag="PLG52_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41138.1"
                     /translation="STPTVSSFGSITPSPVPLTLEITLPPTDTPTITLTIQPTPTYGK
                     VQANEGGGANLRDAPGGTYVMTLLNGTIVETFSEFQVLNNITWVKVYAQVNGQRIEGW
                     LLESVISYATPAPNFQASPTPQGTPTP"
     assembly_gap    1124..1223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1224..1929)
                     /locus_tag="PLG52_06545"
     CDS             complement(<1224..1929)
                     /locus_tag="PLG52_06545"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNB41139.1"
                     /translation="MSIPEEPNLPSFSPQDETPAPPPPIRRRRATRRSHIPTDAEGQA
                     ELIASLARRAYPSIELFVFSLVCGAILGLGYLLDSQALLLLGVLVAPLMTPWVGFLLS
                     ILSGSLRFMFETLMALLISIVFVFLGGLLTGLAALPFMPRTFDNVYLHSRLWIPELIV
                     FALGAIILVISFARSEDKPFLPSVLIAYSFYLPISAAGFGIGSGLPNVWLQGLLVFLV
                     HFAMVSIIGLITLFVLR"
     gene            2040..2930
                     /locus_tag="PLG52_06550"
     CDS             2040..2930
                     /locus_tag="PLG52_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41140.1"
                     /translation="MKEPRVYKTSPILLVFIGLLFLFFIGILLFAIGPNSVGFMIPVG
                     VFSLFLFGAAFISMASKTIVSDDEIAVQGLLGTKNINWANVGRVSGWGYAMKLHSRDD
                     DVTLSISPRLPGYQEVIEFVGSKRPDLFSVQEYGSMKRGITQYLAVLLGLLMMGGVAV
                     AYIVAIMSNEQTITSSSFMPLLIIAVFALIMIVTTLSGPHSINLEGNSVNLKYLFSEK
                     SFQSNEIANVQLSFTQSRNGRHYFIALNLLSRKTIRISGLGMSLPIAYLVLKNWHLGR
                     STGSGAQTNIAPNWSDNTWR"
     gene            3270..>4294
                     /locus_tag="PLG52_06555"
     CDS             3270..>4294
                     /locus_tag="PLG52_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB41141.1"
                     /translation="MTQHRITDDLEALLDVLPANIRHAVEKANNSDKLLEIVIDLGRI
                     PTARFVDGEIALLNTEITRAEIDHITERIGTFDADNRAGMERTLHRISAIKNRRGAVV
                     GLTCRVGRAVYGTVDIIQDLIESGKSVLILGRPGVGKTTLLREAARILAENKRVVIVD
                     TSNEIGGDGDVPHPAVGKARRMQVREPMLQHEVMIEAVENHNPEVIVIDEIGRELEAL
                     AARTIAERGVQLIGTAHGQTLDNLLLNPTLSDLVGGIEAVTLSDEEARRRGTQKTVLE
                     RRAPPTFDVLIEIQTRDRFTVHQDIMTSVDSLLRDAPIPPEVRTRDEAGEIKIEKAAP
                     APKSRSEA"
BASE COUNT         1061 a          980 c         1109 g         1044 t
ORIGIN      
        1 cgcgaatttc gtggatttca catcccgtac cttgcaagaa agatttgatc accccacgac
       61 ctgacccata catggcgtct actacaaagc gttgcggatt gtctgcaatg atgtcagtgt
      121 tgatcagttt gcgaagatgg tcaaaatacg caggcagcgg gttgaagcgt tgaattagcc
      181 cggcatcgcg cgcctttttg aaatccatta agttgggtcc gcgggcttgt tgttcattgt
      241 cattgatgta cacttccacg cggcggcatt gctcggggag tgccgaacca ccaaaagcgc
      301 cctttaattt cacgccgttg tagcgtggtg cattgtgtga tgctgtgatc atcacccccg
      361 caatcgcatt gttgtttttg acagcatatg aaattgctgg cgtgggggaa tctgactgtg
      421 cgagcaaaac gctaaaacca tttgcggcaa gcacacgccc tacttcgcca gcaaaacgat
      481 ctgaaagaaa acgagtgtcg taaccaatta cgatcttttt ctcatccacg ttttcagatt
      541 tatcccagtg ttctgaagca actgcatctg cgatggcttg cgaaaccata cgcaaattac
      601 tgaaagtaaa tgtatctgag atgacggcgc gccagccgtc ggtgccaaaa tgaatggtca
      661 ttgagtctcc tgaagtaaag tgatatgaaa aggtgttgac gtcgtaatag cctgtcacct
      721 taagaacatg aatttatggg gtaggtgtac cttgcggcgt gggcgatgcc tggaagttcg
      781 gtgctggggt cgcataagaa atgacagact ctaacagcca accttcaatg cgctgtccgt
      841 ttacttgggc atatactttc acccaagtaa tgttattcaa tacttggaat tcagagaatg
      901 tttctacaat cgtcccattg agcagggtca ttacataggt gccgcctggg gcgtcacgta
      961 agtttgcacc accaccttca ttcgcctgaa cttttccgta tgtgggtgtt ggttgaatgg
     1021 taagtgtgat cgtgggcgtg tctgtgggcg gcaaagtgat ttctagggtg agtggcacag
     1081 ggctgggcgt aatgctgcca aaagatgaga cagtgggtgt gctnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnngtctcaa aacaaagagc gtgataagac caatgatgct
     1261 taccatggca aagtggacaa gaaacacaag caagccttgc aaccacacat ttggcagacc
     1321 tgagccaatg ccaaaccctg cggcgctgat tggtaagtaa aacgaataag caattagcac
     1381 acttggtaag aaaggtttat cttcagagcg tgcgaatgat atgacaagga tgattgcgcc
     1441 gagggcgaag acgattaatt caggaatcca caggcgcgag tgtaaataga cattatcaaa
     1501 agtacggggc atgaagggta gtgctgccaa cccggtaagc aagcccccaa gaaaaacaaa
     1561 aacgatgctg atgagcaatg ccatgagcgt ttcgaacatg aaacgcaaag acccagaaag
     1621 aatggacaga agaaagccca cccaaggcgt catgagtgga gcaacgagaa cgcctagtaa
     1681 gagcagagct tgagagtcaa gtaaatagcc aagcccaagg attgcgccac ataccaaaga
     1741 gaacacgaag agttcaatgg aggggtaggc gcggcgtgca agcgacgcaa tgagttctgc
     1801 ctgaccttcc gcatcggtag ggatgtgaga acggcgcgtg gcgcgtctgc ggcggatggg
     1861 tgggggtgga gccggggttt catcctgcgg cgagaaggac ggcaggttgg gttcttctgg
     1921 gatggacata tggcaattat acaggaataa cgggtaggaa agagaggtga tggagtatta
     1981 caatatcttc atcgtatagt tgggaccttg tcattcagaa ctgaaatagg agccttatta
     2041 tgaaagaacc acgtgtgtat aagacctcac caatcctttt ggtgttcatc ggtcttttgt
     2101 ttttattctt tattggaata ctacttttcg caatcggacc aaattccgtt gggtttatga
     2161 ttccagtagg tgttttctct ttgtttttat ttggtgcggc atttatctca atggcatcca
     2221 agacgattgt ctcggatgat gagatcgccg tgcagggatt gttgggcaca aagaatatta
     2281 attgggcgaa tgttggcagg gtttcgggct gggggtatgc catgaaactt catagtagag
     2341 acgatgacgt gaccttatcc attagcccgc gtttgccggg gtatcaagag gtgatcgagt
     2401 tcgtcggttc gaagcgacca gaccttttta gtgtgcaaga atatggcagt atgaaacgcg
     2461 gtatcaccca atatctggcg gtcttattgg ggcttcttat gatgggtggg gttgcggtcg
     2521 cctacatagt tgcgatcatg agtaatgagc agactataac ctcctcttca tttatgccgc
     2581 ttttgattat tgctgtgttt gcattgatta tgattgtcac caccctctct ggaccgcatt
     2641 ctatcaatct agaaggaaac tctgtgaatt tgaagtacct gtttagcgaa aaaagttttc
     2701 agtctaatga gattgcaaat gtgcagcttt ctttcacaca aagccgaaat ggcaggcatt
     2761 atttcattgc attgaattta ttgagccgga agaccattcg catttctggt cttggtatga
     2821 gtttgccaat tgcgtatctg gtgctgaaga attggcatct tgggcgttct acgggttcag
     2881 gtgctcaaac gaatattgcc ccgaactggt ctgacaatac gtggcgttag ggtagaactg
     2941 ttcagcatcg cagactagac aatttgcgat gctgaacgga tttaattcgt cagacaaatt
     3001 gatgaaatta atatagattg ctaacaaacc attaatcgcg tacctgctat aatagccttt
     3061 gccactacat aacggcacag gatgcctgac acgcgaaact ctgcgcgtga cgccccagga
     3121 aggcaggtac accatcatga tccttttcaa cttccccctc tttcaccctt aagacaagcc
     3181 gcgtcttttg ttttgcgcga gccgatatat tcttttccct ttgacttttc acgccacttg
     3241 acccttggcg tttctggaaa cttaacatta tgactcaaca tcgaatcaca gacgacctcg
     3301 aagctctgtt ggatgttctg cctgcgaata tccgccatgc ggttgagaag gcaaataaca
     3361 gcgacaaact gcttgagatc gtcattgacc ttgggcgcat tcccactgcc cgctttgtgg
     3421 acggtgaaat tgcgttgctt aatactgaga tcacccgcgc cgagatcgac cacatcacgg
     3481 agcgtattgg cactttcgat gccgacaatc gcgctggcat ggaacgtacc cttcatcgca
     3541 tctccgccat caaaaatcgc cgcggagccg tggttggttt aacctgccgc gtcggtcgcg
     3601 ccgtgtatgg cactgtggat atcattcaag acctgatcga atcgggtaaa tccgttttga
     3661 tacttggtcg cccgggcgtg ggcaagacca cgcttctgcg tgaagccgcc cgcatcctcg
     3721 ctgagaataa gcgcgtagtt atcgtcgata catcgaatga aatcggtggc gatggcgatg
     3781 tgccgcaccc ggctgtgggc aaggcgcgcc gcatgcaagt ccgcgagccg atgcttcagc
     3841 atgaggtgat gatcgaagcc gtggagaatc acaaccccga agtgatcgtc atcgacgaga
     3901 tcgggcgtga gctcgaagcc cttgccgctc gcaccatcgc cgaacgcggt gtgcaactca
     3961 tcggcacggc gcatgggcaa acgcttgata accttttact gaacccaact ttgagcgacc
     4021 ttgtcggtgg catcgaagcg gtgactctct ccgatgaaga agcccgccgt cgtggcacgc
     4081 agaagaccgt gctcgaacgc cgcgcgccgc caacctttga cgtgctgatc gagatccaaa
     4141 ctcgcgaccg ctttactgtg catcaggaca tcatgacctc ggtcgactcg ctcttgcgtg
     4201 acgcgcccat cccaccggaa gtgcgtacac gcgatgaagc gggtgagatc aagatcgaga
     4261 aagccgcacc cgctccaaag tccagaagtg aggc
//
LOCUS       DAOQNJ010000183         4274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13067_length_4274_cov_11.142925, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000183 DAOQNJ010000000
VERSION     DAOQNJ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13067_length_4274_cov_11.142925"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1068
                     /locus_tag="PLG52_06560"
     CDS             67..1068
                     /locus_tag="PLG52_06560"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNB41142.1"
                     /translation="MAELTFQNVLDHLLDSKKDIPAGHLSYYSDLDPKSLRLFLDVWS
                     SVPQKRKLLLLDTLVSHLDEDTIVSYEDIGKALLDDPDAEVRARAMGLLAESDDPKLI
                     DTLLKILKSDSELAPRVKASTLLGEFVLMGELEELDEARLRKIEEALIAIIRSDENPS
                     LRRAAVEAFGYSGRDEAVAILESAYEREDPLWVASALRAMGRSHDNRWDDSVVSKLLD
                     PDPRVRYAAAEAAGELTIEEAGPIMLKMLEDEEEDDNVTMAAIWSLSQIGGEDARAYI
                     LNMLDSSDDDDVTEFLEDALENLDFNDELNKFDLLSLDEDDDLSEFDEDDLDDEDDK"
     gene            complement(1119..2090)
                     /locus_tag="PLG52_06565"
     CDS             complement(1119..2090)
                     /locus_tag="PLG52_06565"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNB41143.1"
                     /translation="MSRPIPADEVRIFSGTSNLPLAKSIAAELDIPLEKTIVKKFSND
                     NLYLQLGASVRYRRVYIVQSLTPPVNDNLMELLMMLDIARDAAASEIHAIIPYYSFGR
                     SDKKDAPRISITARLVADLLRTSGATHVMSMMFHSPQVHGFFRVPTDPLSSRPVFQQY
                     LENRDMSGMIIVSPDMGQAKSAARFAKSLSLPVAAGNKERVSDTEVRISGLVGNQVKG
                     HKRALIYDDEIATGGSIAELTRLLVNEGVEEIMVMCTHGVFTRGGLEKLAAIPQIQEI
                     VTTDTVDITAEKRHPKLTVLSVAKVFADSIRHNFNRQSIGDLFVYGE"
     gene            complement(2136..2804)
                     /locus_tag="PLG52_06570"
     CDS             complement(2136..2804)
                     /locus_tag="PLG52_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNB41144.1"
                     /translation="MIIFLVIAFVFAGLESLALYRNWVKVEWVAKPAVMVCLLIWLFT
                     SVGLDGALLWFGLGLLFSLVGDVLLMISLDTLFLVGLVAFLFAHVFYVIGFNIPLPAI
                     SAWSVILAVLIGWGGAKVIRRILVSVLEKGQGKMRLPIIIYSVVISVMLLSAMVKLND
                     LSWHAGAAILVAVGAFLFYLSDIVLAWNKFVSPFNYGRIYNIALYHLGQIMLIAGVIV
                     QYHR"
     gene            2858..3262
                     /locus_tag="PLG52_06575"
     CDS             2858..3262
                     /locus_tag="PLG52_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB41145.1"
                     /translation="MASKTVYLNWDSESKFVLNDRDNYQLRVNGAGNIGSADLLPMSL
                     IGCSAYDVVEILEKQRQELHQLKVTAEAQQDDTPPWKFRKIHIRYQVIGKGIDLEKAK
                     KAIQLSEEKYCSVYATLRDVIEITHEVEVLEA"
     gene            3313..3726
                     /locus_tag="PLG52_06580"
     CDS             3313..3726
                     /locus_tag="PLG52_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41146.1"
                     /translation="MHRHKRYRYGPPLFPRPFRGWIWMLLIAFMMFGGRGNWWPGILI
                     LVGVMFLFSSLFRDQRPPEPPPPPMQHPPFDMPPPPPARRADTPPNEPVRRADLLPAT
                     CPNCGGPVRSNDLKWTSSHSVACSYCGSNIETRKE"
     gene            complement(3762..>4274)
                     /locus_tag="PLG52_06585"
     CDS             complement(3762..>4274)
                     /locus_tag="PLG52_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41147.1"
                     /translation="VFVYGVSYAGSSWQKFPTPPETPKAIAAAGYSTVVIETEEGHYF
                     YCDTSQLELCWQPENKPSSLIIPVSYGSVEQIEGKPIVPAPGRVVSLVGVDHQDGPNH
                     TEIYYAILKNGSIWYLQLDQSSAGLLAGLSSLIAIPIVFVSSMLFIGMVLAAFLHWLA
                     NRIWREKEAA"
BASE COUNT         1158 a         1150 c          996 g          970 t
ORIGIN      
        1 atcggcttga tgttgttcgt caacatcatg gacttcgtca accctgcaaa cctcacacct
       61 taacttatgg ctgaactcac ttttcaaaac gtactcgatc acctgctcga ttccaaaaag
      121 gatattcctg cgggtcacct ttcctattac tcagacctgg atccaaaatc cttgcgcctc
      181 tttttggatg tttggagcag cgttccgcaa aagcgtaaat tgctcctatt agataccttg
      241 gtctctcacc tcgatgaaga caccattgtt tcgtatgaag acatcggcaa agcgcttctg
      301 gatgacccgg atgcggaagt ccgcgcccga gctatgggtc tgctcgccga atcagacgac
      361 cccaagctta tagataccct cctgaagatt ctcaaatctg acagcgagct tgctccgcgt
      421 gtgaaagcct ccacgttgct tggcgagttc gtgttgatgg gtgaactcga agagcttgat
      481 gaagctcgtt tgcgcaagat cgaagaagcg ctgatcgcca tcattcgcag cgacgagaat
      541 ccaagcctgc gccgcgccgc cgtggaagcc ttcggctact ctggtcgtga tgaggcggtg
      601 gcgatccttg aatcagcgta tgagcgtgaa gatccgcttt gggtggcatc tgctctgcgc
      661 gccatgggac gttcgcacga taaccgctgg gatgatagcg tggtcagcaa actgcttgac
      721 cccgatccac gcgtccgcta tgctgccgcc gaagccgccg gtgaactgac catcgaagaa
      781 gcgggtccta ttatgctgaa gatgctcgaa gatgaagaag aggacgataa cgtgaccatg
      841 gcagccattt ggtctctgag ccagatcggc ggcgaagatg cgcgtgccta catcctcaac
      901 atgctcgata gctccgatga tgacgatgta acggaatttc tcgaagatgc gctcgaaaat
      961 ttagacttca acgatgagct aaacaagttt gacctgctct cgctcgatga agatgacgac
     1021 ctttctgaat tcgacgaaga cgacctcgac gatgaggatg ataaataaaa caaaaagaca
     1081 ctcccttact gggagtgtct ttttgtttgc gctgagagtt attcgccgta cacgaacagg
     1141 tcaccaatcg actgtcggtt gaagttatgg cgaatggagt ctgcaaagac ctttgccacc
     1201 gaaagaacgg tcagcttggg gtgacgtttc tctgcagtga tatcgacggt atcggtcgtt
     1261 acaatttctt gaatttgcgg aatcgccgcc agtttctcca acccgccgcg ggtaaagaca
     1321 ccatgggtac acatcaccat gatttcttcc acgccttcgt tcaccagcaa acgcgtcaat
     1381 tcagcaatgg agccgccggt agcgatctca tcatcataga tcaaagcgcg tttgtgccct
     1441 ttaacttggt taccgacaag tccgctaatg cgcacttcgg tatcggagac gcgttcttta
     1501 tttcctgccg ccacaggcag gcttaaactc ttcgcaaagc gcgccgcaga cttggcttga
     1561 cccatatcgg gcgaaacgat aatcatgccg ctcatatcgc ggttttccaa atattgctga
     1621 aagaccgggc gactgctcag ggggtctgta ggcacacgga agaacccatg cacctgcggc
     1681 gagtggaaca tcatgctcat cacatgcgtg gcaccagagg ttctgagcag gtccgcaacg
     1741 aggcgggctg tgatggagat gcgcggcgcg tcttttttat cggaacgccc aaaagaataa
     1801 taaggaataa tggcatggat ctcagaagcg gcggcatcgc gcgcaatatc gagcatcatc
     1861 aacagttcca tcaaattatc gttgaccggc ggcgtgagcg actggacaat gtacactcgg
     1921 cgatagcgca cgctagcacc aagctgcaaa tacaaattat cgttactgaa cttcttcacg
     1981 atggtctttt ctaatggaat atccagctct gcggcaatgc ttttggcaag cggcaaatta
     2041 gacgtgccac tgaaaatgcg aacttcatca gcggggatgg gacgcgacat atgattctcc
     2101 atataccttg aattgtattc cgattatgac acaaatcagc ggtgatactg aacgatcacc
     2161 ccggcgatca acatgatctg accaagatga tacaaggcga tgttatagat acgcccataa
     2221 ttaaagggcg agacgaactt attccatgcc agaacgatat cggaaagata aaacaaaaac
     2281 gcgccaaccg ccacaagaat agctgccccc gcatgccagg ataaatcatt gagtttcacc
     2341 atggcagaaa gcagcatcac tgaaatcacc acactgtaga tgatgatcgg caggcgcatt
     2401 tttccctgcc ccttttcaag gacagaaacc agaatgcggc gaatgacctt tgctccgccc
     2461 caaccgatca acacagcaag aatcacactc cacgctgaga tggcaggcaa agggatatta
     2521 aacccgatca cataaaacac atgcgcaaat aagaatgcca ccagtcccac taaaaataac
     2581 gtatcgagcg agatcatcaa taacacatca cccaccaaag aaaagagcaa ccccaaacca
     2641 aaccataaca acgccccgtc taatccgaca gaggtgaata accagatgag taagcaaacc
     2701 attaccgccg gtttggcaac ccattccact ttcacccagt ttctatacaa tgccagagat
     2761 tccaaccctg caaatacaaa cgcaatcact aaaaagataa tcacagtgcc ctcctgctct
     2821 atcctaccac gaaggcatcc catgctatac tgcggatatg gcatcgaaaa ctgtatatct
     2881 caactgggac tcagagtcca aatttgtact caacgataga gacaattatc aattacgcgt
     2941 caatggcgcg ggaaatattg gctccgccga tttgttaccc atgtcgctga tcggctgttc
     3001 ggcatatgac gtggtcgaaa ttctggagaa acagcgtcag gaactgcacc agctcaaggt
     3061 cacggcagag gcgcagcaag atgacacacc accctggaag tttcgaaaga tccacattcg
     3121 gtatcaggtc attggcaaag gcatcgacct tgaaaaggca aagaaagcca ttcaactcag
     3181 cgaagaaaaa tattgctcgg tgtacgccac cctgcgcgat gtgatcgaga tcacgcacga
     3241 ggtagaagtg ttggaagcat agcaacttca atgagggtgg cgcgtatagt atgcgtaccc
     3301 aaaggatatt tcatgcacag acataaacgt taccgttatg gaccgcccct cttccctaga
     3361 ccctttcgcg gctggatctg gatgctctta atcgctttta tgatgttcgg cggcaggggc
     3421 aattggtggc ccggcattct catcttggtt ggcgtcatgt ttctctttag ctctctcttt
     3481 cgagaccaac gcccgcccga gcctccacca ccgcccatgc aacacccgcc atttgacatg
     3541 cctccgcctc cccccgcccg gcgagcggac acgcccccaa atgagccagt gcgccgcgca
     3601 gacctgttgc ccgccacctg ccccaactgt ggtggtcccg tccgctcaaa cgacctgaag
     3661 tggaccagtt ctcactccgt cgcctgctcg tattgcgggt cgaacattga aacgagaaaa
     3721 gaataaaaaa agctctgggt tttgaaccca gagctttttc tttatgcggc ttctttctcg
     3781 cgccagatgc ggtttgccaa ccaatgcaag aatgcagcaa gcaccatccc tataaagagc
     3841 atgctgctca caaaaacaat aggaattgca ataagagaag aaagacctgc cagtaaacct
     3901 gctgaactct gatccaattg caaataccaa atactgccat tctttagaat cgcataatag
     3961 atttcagtat ggtttggacc atcttgatga tctacgccta ccaaactcac aactctccca
     4021 ggggcaggga caattggttt gccttcaatt tgttctactg agccgtaaga cacagggatg
     4081 atcaggctac tcggtttatt ctctggctgc caacacaact caagttgaga cgtatcgcaa
     4141 tagaaataat gtccctcttc tgtttcaatc actaccgtac tatacccggc tgcagcgatt
     4201 gcctttggtg tttcaggcgg ggtggggaat ttttgccaac tagaaccagc atatgaaaca
     4261 ccgtacacaa atac
//
LOCUS       DAOQNJ010000184         4271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13082_length_4271_cov_7.640655, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000184 DAOQNJ010000000
VERSION     DAOQNJ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13082_length_4271_cov_7.640655"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLG52_06590"
     CDS             <1..298
                     /locus_tag="PLG52_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41148.1"
                     /translation="SLKAFGIPGEVIYTPGHSMGHVSILLETGDALVGDMAMNDWYLR
                     LTPGLPVLADHIEMVVESWKKILPLGIKRVYPAHGMDFDVDVMQKEIAAFKPRK"
     gene            complement(336..584)
                     /locus_tag="PLG52_06595"
     CDS             complement(336..584)
                     /locus_tag="PLG52_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41149.1"
                     /translation="MSAENDIIALRSRVAELERKLDFIYRRMGIEYIDDPGMVDSQII
                     SLLKAGNKLEAIKVYRELTNADLLDAKRAVEKIEASVL"
     gene            complement(649..1278)
                     /locus_tag="PLG52_06600"
     CDS             complement(649..1278)
                     /locus_tag="PLG52_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41150.1"
                     /translation="MKNQTPLLLTVSLILLLAAACTPKATQDPNLLIQQAVNATLASI
                     PTATQVSPPTPYPSPTPFTLAGLFCEYQFCIGHPIDVSFFDVSAQQNPASPSTYGQGI
                     LAALNGNLFIQMMWQLAPGTSDPKFLFDTLLNDSVDTATNTSSIFLVRDMNVYYDDIT
                     TLATPTLPYGGAAAWTCGDRVFAWKVYTPQSGTARGIFDETLARFTCGQ"
     gene            complement(1334..3118)
                     /gene="nirK"
                     /locus_tag="PLG52_06605"
     CDS             complement(1334..3118)
                     /gene="nirK"
                     /locus_tag="PLG52_06605"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HNB41151.1"
                     /translation="MKPYFKYFIFFILLGIFLAACAGADFQQVDKEYVLTTDLRNGNL
                     VFLGVSDEINGQENPTLSAKAGERITITLINGGEGEHSITLPELNASSEIVKEKGEET
                     SISINVPNVHGEIGYYDGVGNHADLGMRGKLVVTETGAAMPAMNTTTTSNGDPAVLAA
                     FQKGACGSCHTISGIPGAVGVVGPKLDDISVMSEEMIKDTSYSGHATNAEEYIRESIT
                     DPNLFLAPECPTGACAPNVMPATLTQTLNEEEINAIVTYLTGLPDGAYKESTTTTEAV
                     LAPQEPDNSGVDIIRDPTDIPAPVGNREPQIVRIDLEATEVVGQLADGTTYTYWTFNN
                     QVPGPFFRVRVGDTIEVHMKNSLSSTMNHSVDFHAVTGPGGGAVMTQTEPGKETVFTA
                     KAINPGLFVYHCATPMVAQHITNGMYGLILVEPEGGLPPVDREFYVMQGEIYTAGAFG
                     DQGHQMSDTTKLLGEEPEYFVFNGAVNALKDKKPLKANVGESIRIFFGVGGPNFTSSF
                     HVIGEIFDRVYDQASLTSAPLTNVQTTLVPPGGATMVEFNVEVPGNYILVDHALSRLQ
                     RGLAGVLIVEGDQNPEIYNGVPSAGSGH"
     gene            complement(3115..3366)
                     /locus_tag="PLG52_06610"
     CDS             complement(3115..3366)
                     /locus_tag="PLG52_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41152.1"
                     /translation="MRYSVATMNDELEISEIHVESILKAQPNAARFFLNWKLGCVGCG
                     FARFCTLADVIHTYKLDEKKFLEEAKQLVVHHTLMRSSE"
     gene            complement(3425..4123)
                     /locus_tag="PLG52_06615"
     CDS             complement(3425..4123)
                     /locus_tag="PLG52_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB41153.1"
                     /translation="MKISPQDLKQVVVFQHATDEDLELILKNSITRSIEEGSFFFMQG
                     DEAHYLYILTSGQVKLMQSNPNGQQVNLRTIYPWQMFGALGAVRTDGAAYPASAQTLE
                     DSTALATPSLFLRSMLETRPYLSFDLMNLMTSYIQEMQARYRELATERVEQRVANALV
                     RLAGQSGIRSDKEAGIELSFSRQDVAEMTGTTLYTVSRLLSDWERKGIIKTGREKILV
                     LNPHELVRIADGQV"
BASE COUNT          991 a          970 c         1196 g         1114 t
ORIGIN      
        1 gtcgctgaaa gcgtttggta ttccgggcga agtgatctat accccggggc attcgatggg
       61 gcatgtgagc attttgcttg aaactggcga tgccttggtg ggtgatatgg caatgaacga
      121 ttggtatttg cgcctaacgc ccggcttgcc tgtgcttgcc gaccatattg agatggtcgt
      181 agaaagttgg aagaagattc tgccactagg aataaaacgg gtatacccgg cacatggcat
      241 ggattttgat gtagacgtga tgcaaaaaga gattgcggcg ttcaagccaa gaaagtgaat
      301 caaaaacgcc agagaacatt ctctggcgtt tggttttata aaacactggc ttctattttt
      361 tcaacagccc ttttggcatc taacaaatcg gcgttggtca attcgcgata gaccttgatg
      421 gcttcaagct tgttgcctgc cttcaacagc gaaatgatct gtgaatcgac cataccgggg
      481 tcatcaatat actcaattcc cattcttcta tatataaagt cgagcttcct ttccagttca
      541 gcaacgcgtg agcgcaatgc aataatatcg ttttcagcag acatgtttat ctccttcaaa
      601 acgatattac accttatagg tcaggttggc attcacctat ttttcctatt attgtccgca
      661 agtgaagcgg gctagggttt catcaaaaat gccgcgtgcg gtgccagatt gaggggtgta
      721 gactttccaa gcgaagacac ggtcgccgca agtccaggcg gcggcgccac cgtagggcag
      781 ggttggcgta gcgagggtgg taatgtcatc atagtacacg ttcatatcac ggacgagaaa
      841 gatgctggaa gtgttcgtag ccgtatcaac gctgtcattg agcagggtat caaaaaggaa
      901 ttttgggtct gacgtgccgg gcgcgagctg ccacatcatt tggatgaata gattgccatt
      961 gagcgcagcc aagatgccct gaccataggt gctgggcgaa gctgggtttt gttgggcgct
     1021 tacatcgaag aaggaaacgt cgatcgggtg accgatacag aattgatatt cgcagaataa
     1081 gcctgctaat gtgaacggtg taggggaagg gtagggtgtg ggaggcgaaa cttgtgttgc
     1141 agtaggaatg gaagccagcg tggcgttgac ggcttgttgg atgagcaggt ttgggtcttg
     1201 agtcgctttc ggtgtgcagg cggctgcgag aagcaaaatc aggctcaccg ttaagagaag
     1261 aggcgtttgg tttttcatcc gcgaaattat aaaggaaaag ccccgtccaa tttcgacggg
     1321 gctttggttt ggtctagtgt ccgctgcctg cagatgggac accgttataa atttctgggt
     1381 tttggtcgcc ttcgacgatg agcactccgg cgagaccgcg ttgtaatctg gataaggcgt
     1441 ggtctacaag gatgtagttg ccgggcacct cgacattgaa ctcaaccatg gtggcgcctc
     1501 cgggcgggac gagggtggtt tgcacgttgg tgagcggcgc actggtgaga gaggcttgat
     1561 cgtagacgcg gtcgaagatt tcaccgatga cgtggaaaga ggatgtgaag ttcggtccgc
     1621 cgacaccgaa gaagatgcga atgctttcgc cgacattggc tttgagcggc ttcttgtctt
     1681 tgagggcatt gacggcacca ttgaaaacga agtattcagg ttcttcaccg agcagtttgg
     1741 tggtatcgct catttgatga ccttgatcgc cgaatgcgcc agcggtgtag atttcgcctt
     1801 gcattacgta gaactcgcgg tctactggag gcagcccacc ttcaggttca acaaggatga
     1861 gaccatacat cccattggtg atgtgttggg cgaccatggg tgtagcacaa tgatagacga
     1921 ataaaccggg gttgatcgct tttgcggtaa agactgtttc tttgccgggt tcggtttggg
     1981 tcatcactgc accgccgcca ggtccggtca cggcatggaa atcgaccgag tgattcatgg
     2041 tgcttgaaag gctgttcttc atatgaactt cgatcgtatc gccgacacgc acgcggaaga
     2101 acggtccggg gacttggttg ttgaaggtcc aataggtgta ggtggtgccg tctgcgagtt
     2161 gaccgacaac ctcagtcgct tcgaggtcga tgcgtacaat ttgcggttca cgattgccaa
     2221 cgggggcggg gatatcggta gggtcacgga tgatatcgac accgctgtta tcgggttctt
     2281 gtggagccaa tactgcttct gtagttgtcg tggattcttt gtacgcgcca tctggaagac
     2341 cagttagata tgtgacgatg gcgttgattt cttcttcgtt cagggtctga gttagagtgg
     2401 cgggcatcac gtttggagca caagcaccag tggggcactc aggcgcgagg aagagattcg
     2461 ggtctgtaat tgattcgcgg atgtattctt cagcattggt cgcatgacct gaatagcttg
     2521 tatctttgat catctcttca gacatgacgc tgatgtcatc gagctttggt ccgacaacgc
     2581 cgacggcacc tggaattcca gaaatggtat ggcaggaacc gcaggctccc ttttggaagg
     2641 cagcgagcac ggcagggtca ccgttgctgg tagttgtggt attcatggct ggcatcgctg
     2701 cgccggtttc ggtcacaact aatttgccgc gcatgcccaa atctgcatgg ttgccgacac
     2761 cgtcgtaata gccaatctca ccatgtacat taggtacgtt gatgctaatg gatgtttctt
     2821 cgcctttttc tttcacgatc tcagaactgg cgtttagttc tggaagagta atgctatgct
     2881 cgccttcacc gccgttgatg agggtgatgg tgattcgttc ccccgcttta gcgctgagcg
     2941 taggattctc ctgcccgttg atctcgtcac ttacgccgag aaagaccaaa ttgccgttgc
     3001 gtaagtcggt cgtcaagaca tattctttgt ccacctgttg aaagtctgcg cctgcacagg
     3061 cagcaagaaa aatgccaagc aggatgaaaa atataaaata cttgaaatag ggtttcattc
     3121 tgaactcctc attaaggtat ggtgaacgac aagttgtttg gcttcttcca gaaatttttt
     3181 ttcgtcaagc ttataagtgt gaatgacgtc tgccaatgtg cagaagcgag caaaaccgca
     3241 gccaacacat cccagtttcc agttcaagaa aaaacgggcg gcattgggct gagcttttag
     3301 aatgctctcc acatggatct cagaaatttc cagttcatcg ttcatagtgg ctacagaata
     3361 tcgcattccc ccaaaccctt ctttgcgctg gcgcaaataa aaagagcgca accttgctgc
     3421 gcccttacac ttgtccgtct gctattctta ccaattcatg cgggttgaga accagtatct
     3481 tttcccgccc ggtcttgatg atgcctttcc gctcccaatc actgagcagg cgactgactg
     3541 tgtacagcgt ggtgccggtc atctctgcca catcttgccg ggagaaggat agttcgatcc
     3601 ccgcttcttt atcggaccgt atgcctgatt gcccagccaa gcggaccagc gcgttagcca
     3661 ctctctgctc gacccgctcg gtagcaagtt cgcggtaacg tgcctgcatc tcttggatgt
     3721 agctggtcat taggttcatt aagtcaaagg aaaggtaggg gcgagtttcg agcatggagc
     3781 gcagaaacag gctcggggtc gcaagagccg tgctgtcctc caaggtttga gcggaagcag
     3841 ggtaggcggc tccatccgtg cggacggctc ccaatgcgcc gaacatctgc cacgggtaga
     3901 tcgtccgcag gttcacttgt tgcccgttgg gatttgactg catgagcttg acctgtccgc
     3961 tggtgaggat gtacagataa tgggcttcat caccctgcat aaagaagaaa ctgccttctt
     4021 ctatggagcg ggtaatgcta ttttttagaa taagttcaag gtcttcatcg gttgcgtgtt
     4081 gaaaaacaac gacctgttta agatcttgag gggaaatttt cataaagtca ttttacaccc
     4141 caatccgatg acatttatcg acttgacgag tgagatgggg atggcttata ttgtgcctat
     4201 ccctaccccc ctgggggggg taagagaaac caaacggact gtatctggaa actagaggta
     4261 actatggaaa a
//
LOCUS       DAOQNJ010000185         4262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13131_length_4262_cov_9.369384, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000185 DAOQNJ010000000
VERSION     DAOQNJ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13131_length_4262_cov_9.369384"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1615)
                     /locus_tag="PLG52_06620"
     CDS             complement(164..1615)
                     /locus_tag="PLG52_06620"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNB41154.1"
                     /translation="MEQKAGAQIGKRAFIQSLVILFVLMMVAGILTLAIPSGQYTRIE
                     VDGRETIDPNSFQLTERPNYPIWRWFIAPIEVLGSDSGLTVIVIIVVLFFAGGAFAVM
                     DKTGTLRAFIGRIVRRYSGRKYTLLWMVSLSFMFLGATLGTFEEVVLLVPVMIALSYS
                     LGWDALVGLGMSILATNMGFSAAISNPYTLGVSQQLAGLPLFSGAWYRIIIFAVIYFI
                     FMWFLSSYAKKIDKDPTSSLVHKEDNAEREKYKSMEDASFTEDNPKQNRAMNFFGFFL
                     IFIFVVMLSGPFVPAISDYALPIVGILFLIGGLGAGFISGAGGKTVWQGLIEGWGGLA
                     PSVPLILMAASIRFIIDSGGVTDTILHAAADPFKQLGAFPAALVVYALALGIEFFIAS
                     GSAKAFLMIPIVLPLADLIGVTRQTAVLAYIFGDGFSNLAYPTNPVLLISLGLTVVSY
                     PKWLRWTAKLWLWVILATVTFLGIAVAIHFGPF"
     gene            1741..2226
                     /locus_tag="PLG52_06625"
     CDS             1741..2226
                     /locus_tag="PLG52_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HNB41155.1"
                     /translation="MIESENAFLEFLNAKGFVYSRMEHPAVFTCAEADTHHIGLDAVS
                     TKNLFLCDKKARRFFLAVTACEKTVRLDELSGLFGVPHLRFASEQNLLRLLGVAGGSV
                     TMMGLANDTEHIVELWIDNEIWQAENFLCHPLVNTATLTLTKAELERFLALTGHTPKF
                     F"
     gene            complement(2233..3081)
                     /locus_tag="PLG52_06630"
     CDS             complement(2233..3081)
                     /locus_tag="PLG52_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41156.1"
                     /translation="MSKQTLKNNFFELEYLTHSLRISGITPHGKTNLLADLSHQPPVP
                     TPYGDFYFRGGHRLWHAPEAMPRTYVPDTGEIKITNIPNGVVLETETEPGANIRKRIE
                     IKLAVDTPSLTLTHTLFNDGLWTLELAPWAITQFRLGGIAILPMPTEKVDDAALLPNR
                     QISFWTYTRLNDKRLSLRDEFSLFHAESSLPPFKMGYFNPHGWLAYYIDGVLFKKTFN
                     ASTLAPYPDNNCNAELFCNDEFVELESLGPLTKLEPGASVQHQETWEILDANALPQDI
                     QNLILK"
     gene            complement(3081..4055)
                     /locus_tag="PLG52_06635"
     CDS             complement(3081..4055)
                     /locus_tag="PLG52_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB41157.1"
                     /translation="MIYGEVEGIKKPIARVVQGTTMLGSDLNEEESFSLLDQVYELGG
                     NTLDTAHVYAGGNSERIIGKWMHSRGLRKKMTIITKGGAHSQDRRRMTSFDVTSDLFD
                     SLARLKTDYIDLYLLHRDDTNLPVEPIIHTLNEHLQAGRIHAFGASNWSHQRMQDAAT
                     YAKANGLKTFIASSPQYSLADSNAEPWPLCLSISGDTGKEAREWYANTAMPVLVWSPL
                     ASGFFSGRFRRDNLREFGEREWDEVVVRTYATEANFQRLDRAEALAKEKGCTAAQIAL
                     AYVMNQPMNVFSVVGSHSIEKFKANIEAAELKLTNQELAWLDLKVENL"
     gene            complement(4083..>4262)
                     /locus_tag="PLG52_06640"
     CDS             complement(4083..>4262)
                     /locus_tag="PLG52_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB41158.1"
                     /translation="LRWILMHDAVTCTIPGAKNPAQAEDNVKAADQPSLSPEAMEKIK
                     AVYDSQIRSLVHNYW"
BASE COUNT         1150 a          964 c         1044 g         1104 t
ORIGIN      
        1 actgcaaatg tgatgacagg cacccgtgaa ttttgtttgc aagctggtat ggatgattac
       61 ctgagcaaac cgatcacgtt acagtctatg cgtgaagtct tggaaaaatg gctcgtttga
      121 tataaaaaaa gcctgcacaa aatgtgcagg ctctttttat gctttagaac ggaccgaaat
      181 gaattgcaac agcaatccct aaaaatgtca cagttgctag aattacccag agccacagtt
      241 tggctgtcca acgtagccat ttggggtaac ttactacggt gagaccaaga ctaatgagca
      301 agaccgggtt ggtgggatag gcaaggtttg aaaagccgtc gccaaagata tatgccaaaa
      361 ccgcagtctg acgggtgaca ccgatcaagt ctgcgagagg gagaacaatg gggatcatca
      421 agaatgcttt agcggagccg gaggcgatga agaactcaat ccctaatgca agggcataaa
      481 caactaaggc ggcaggaaaa gcaccaagct gtttaaatgg gtctgcggct gcatgcagaa
      541 tggtatcggt cacaccgccg ctatcgataa tgaaacggat actggctgcc atcaaaatga
      601 gtgggacgga aggagcgagc ccaccccaac cttcgatcag cccctgccag acggttttac
      661 cgcccgcacc agagataaag ccagcaccta atccgcctat caagaaaaga atacccacaa
      721 tgggcaaagc ataatctgag atcgcaggca cgaacggacc tgaaagcatg acaacaaaaa
      781 tgaagatcag gaagaacccg aagaaattca tggcgcggtt ttgtttgggg ttatcttcag
      841 taaacgaggc atcttccatg ctcttatatt tctcgcgctc ggcattgtct tctttatgca
      901 ccaaagagga ggtagggtct ttgtcgattt tttttgcgta agaagacaaa aaccacatga
      961 agataaagta aattactgca aagatgataa tgcgatacca ggcacccgag aaaagaggta
     1021 accctgcaag ttgttgagaa acgccaagcg tatacggatt ggaaattgca gccgaaaatc
     1081 ccatattcgt tgcgagaatg gacatgccca accctaccaa cgcatcccag ccaagcgaat
     1141 aggatagtgc gatcataacg gggactaata gcacaacttc ttcaaatgtt cccaaggttg
     1201 cgccgaggaa catgaaagat agagagacca tccatagcaa ggtatatttt cgtccactat
     1261 atctgcggac aatgcgaccg ataaaagcac gcaaggttcc ggtcttatcc atcacagcga
     1321 aagcgccgcc ggcaaagaac agcacaacga taatgacgat gacggtcaaa ccgctgtcac
     1381 tgccaaggac ttcgattgga gcaatgaacc aacgccagat agggtagttt gggcgttctg
     1441 tgagttgaaa tgaatttggg tcgattgtct cgcgcccgtc tacttcaatc cgcgtatatt
     1501 gccccgatgg gatggcgagg gtcaatatac ctgcgaccat cattaaaacg aacaagatca
     1561 ccagcgattg aataaaagca cgtttaccga tctgagcacc agctttttgt tccatggttt
     1621 ggctccttgg gaaaagttag agacactgta ccacaaagtg cttgttgtga ttgttaaatc
     1681 tagaggaaag ttggctcgct tttacgcgcc tttcgcggta tcatgccaac cctatcgctt
     1741 atgatcgaat ctgaaaatgc cttccttgaa tttttgaacg ctaaggggtt tgtttattct
     1801 cgcatggagc atccagccgt cttcacctgc gccgaagcgg atacccacca tattggtttg
     1861 gatgcggtaa gcaccaaaaa cctttttctg tgtgataaaa aagcccgtcg cttctttttg
     1921 gcagtcacgg cttgcgaaaa gacggttcgg ctcgatgaac tctcagggct atttggtgtc
     1981 cctcatttgc gctttgcatc tgaacagaac ttgctgcgcc tcttaggtgt ggcaggcggc
     2041 tcagtgacga tgatgggctt ggcaaatgat acagaacata tcgtcgaatt gtggatcgac
     2101 aacgaaattt ggcaggcaga gaactttctt tgccatccct tagttaacac agccaccttg
     2161 actttaacaa aagcggaact ggagcgcttc cttgctctga ccgggcatac gcctaaattt
     2221 ttttagtgcg ggctatttca gaatcagatt ctgaatatct tgcggtaatg catttgcatc
     2281 aaggatttcc catgtttcct gatgttgcac cgatgcaccg ggttccagct ttgtgagcgg
     2341 acctaaactt tccaattcca caaattcatc attacagaaa agctcggcat tgcaattgtt
     2401 atctgggtag ggtgcaagtg ttgatgcgtt aaacgttttc ttgaagagaa cgccgtctat
     2461 ataatatgcc agccagccat gcgggttgaa atagcccatt ttgaaaggcg gcaaagaaga
     2521 ctcggcgtga aaaaggctga attcatcgcg caggcttagg cgtttgtcgt tgaggcgggt
     2581 gtaggtccaa aaagatattt ggcggttagg aagtaatgcg gcatcatcca ctttttcagt
     2641 gggcatgggc aaaattgcta ttccacctaa tcgaaattga gtgatcgccc atggtgccag
     2701 ttcgagagtc cacaagccgt cattgaaaag ggtgtgggtg agggtgagag agggtgtatc
     2761 tactgcaagt ttaatttcga tccgcttgcg aatgttggca ccgggctctg tttcggtttc
     2821 aagcacaaca ccattgggaa tattagtgat ctttatttca ccagtatcgg ggacataagt
     2881 gcgcggcatg gcttcggggg catgccataa acgatgcccg cctcgaaaat agaaatcgcc
     2941 gtaaggggtt gggacgggcg gctgatgact tagatcggct agcaaattgg ttttgccatg
     3001 cggggttatg ccagagatac gtaaagaatg tgtgagatat tcaagctcaa agaaattgtt
     3061 ttttagggtt tgtttgctca ttatagattt tctactttca ggtccagcca agccaattct
     3121 tggttggtga gtttcaattc agcggcttca atgtttgctt tgaacttttc aatactatga
     3181 gaaccaacaa ctgaaaaaac attcatcggt tggttcatta catacgctaa tgcgatttgc
     3241 gcggctgtgc atcctttttc ttttgccaaa gcttcagcac ggtctaaacg ttgaaagttt
     3301 gcttcggtgg catatgttcg cacaactact tcatcccatt cgcgttcacc aaactcgcgc
     3361 aggttgtctc ttcgaaaacg cccagagaaa aacccactgg caagtggaga ccacaccaaa
     3421 accggcattg cggtgttggc ataccattct ctggcttctt tgccagtgtc accgctaatg
     3481 ctcaaacata agggccaagg ttcagcgttt gagtctgcta ggctgtattg tgggctgctg
     3541 gctatgaatg ttttcaagcc attggctttt gcataggttg cagcatcttg catccgttgg
     3601 tgagaccagt tcgatgcgcc aaacgcatga atgcgacccg cttgtaaatg ttcattgagt
     3661 gtgtgaataa tcggctcaac cggcagattg gtatcatcac gatgcaacag gtaaagatca
     3721 atgtaatctg tcttgaggcg tgcaagtgag tcgaataggt cagaggtaac atcaaaagac
     3781 gtcattctgc ggcggtcttg tgaatgtgcg ccgcctttgg taatgatggt cattttctta
     3841 cgcaatccgc gcgagtgcat ccattttcca ataatgcgtt cgctgtttcc accagcgtac
     3901 acatgcgctg tatcgagtgt gttaccgcca agttcatata cttgatctaa aagagaaaaa
     3961 ctttcttcct cattcaaatc actgcccaac atagttgttc cttgcacaac acgggcaatt
     4021 ggctttttta tgccttcaac ctcgccatag atcatcaaac acctctatcg attggatttg
     4081 ttttaccaat aattgtgaac gagactgcgg atctgagagt cgtacacggc tttgattttt
     4141 tccatcgcct ctgggctcag ggacggctgg tctgccgcct tgacattatc ttccgcttga
     4201 gccggattct tcgcgccggg gatagtgcaa gtgacagcat catgcatcag gatccagcgc
     4261 aa
//
LOCUS       DAOQNJ010000186         4247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13200_length_4247_cov_9.130010, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000186 DAOQNJ010000000
VERSION     DAOQNJ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13200_length_4247_cov_9.130010"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLG52_06645"
     CDS             complement(<1..635)
                     /locus_tag="PLG52_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41159.1"
                     /translation="MEEIAQTPSGENIIPPLLDRTWKRILFGLFVVAAPIFNFSLIEL
                     MKPEWQDGRFTSYLYLFLLPEASWGFFPLLLYAILSYLLLLWDSEQFADSMIIRLGIY
                     GGTLLALQYSILSFIATFSVSPMVLAVILAYSAPLLLPKLYFWLRPQLDKLFKMYRLA
                     MIIIILIIIALVLWGFFFVLAIFGIASPFWSFLVAFQASRWLLKHYETKFS"
     gene            complement(648..1472)
                     /gene="rsmI"
                     /locus_tag="PLG52_06650"
     CDS             complement(648..1472)
                     /gene="rsmI"
                     /locus_tag="PLG52_06650"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNB41160.1"
                     /translation="MGTLYLVATPIGNLEDMSPRAVRILREAILIAAEDTRHTGTLLK
                     HFNVETPLTSYFEHNKLTKQDFILEKLAQGDVALVSDAGTPAINDPGYELVQAALASG
                     FDVRPVPGPSAPISALTVSGLPTDSFLYLGYLPHKSSERRGFAGEVSGLEYTLIFLET
                     PHRLVDSLEDLLAMLGNRRICVAREMTKLYEEYWRGDISGAIEYFKAKEARGEFTLVI
                     EGRKKEERTVWAESQLIEVIKHELDAGKSAKEISADLAEKSGWNKKEVYALVNQVK"
     gene            complement(1526..3004)
                     /locus_tag="PLG52_06655"
     CDS             complement(1526..3004)
                     /locus_tag="PLG52_06655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB41161.1"
                     /translation="MPLPEKIGRYEVKSELGRGGMATVYRAVDPNSGREVAVKVLPRE
                     MMHDPQFRERFEREIKMIASLEHPAIVPVYDVGDIDGQPYFVMRFMTGGSLSDLIEKG
                     SIPIDETAKIIEKIAQGLAYAHRKGVIHRDLKPDNILFNDDGEPFISDFGVAKLSESS
                     SNLTGSGVIGTPAYMSPEQAQGNEIDQRSDVYGLGVIVYQMLSGHQPYSADTPMGVVV
                     KHITEPVPEILKVLPNLPEDVDAIIKTAMAKDKTQRYANTIELAKALNTVAFGTPGDI
                     TFHTMNGRTRLPSNSKPLSRGQTTGLAVGGVILLLAIVGVFLLRNQLFASSSPTSTTD
                     AASTEVSETSTPVLVVVTATLPEATLASGPASAPACSANVSFPAPIGKETNSFCTQKI
                     PYAYANMADHATFTILDSSTAGAKCRQEAIKNGEKVVSCTGPSFAVIDLEVCEPVELK
                     PEELSQCSADSTYNAANQCCVQQPPQKASCKVIKIQLKGCGG"
     gene            complement(3104..3502)
                     /locus_tag="PLG52_06660"
     CDS             complement(3104..3502)
                     /locus_tag="PLG52_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB41162.1"
                     /translation="MPALNRVQLIGRLGKDPESKYTPTGKKVCHFSLAVSNRWKDKNG
                     ETKETTEWVNIETWGRLGEVCQEYLKKGSLIFVEGRLKTDKYEDRESGETRYYTKIIA
                     QSLQFLDRKASEEPVMAVEEESGEYELAIE"
     gene            complement(3634..3855)
                     /locus_tag="PLG52_06665"
     CDS             complement(3634..3855)
                     /locus_tag="PLG52_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41163.1"
                     /translation="MAFGYTNSKGTTYYLHARGKMFFFAKEVKEGALDAVPAGYNVVE
                     MKTGMPVLKKAVAEGSATTTETTNTESAG"
     gene            3957..>4247
                     /locus_tag="PLG52_06670"
     CDS             3957..>4247
                     /locus_tag="PLG52_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNB41164.1"
                     /translation="MKIVSSLSDLRAARLSLDGTVGLVPTMGYLHEGHLSLVSRAKAE
                     NQHAIVTIFVNPTQFGPNEDLSKYPRDLERDLDLLRPLGVDVVWTPTAEVMYP"
BASE COUNT         1063 a          988 c         1050 g         1146 t
ORIGIN      
        1 aaagaaaact tggtttcgta gtgtttcaaa agccaacgag aagcttggaa ggcaacaagg
       61 aaagaccaga agggtgaagc gatcccaaag atcgctaata caaagaaaaa tccccacaga
      121 acaagggcta tgatgatcaa tataatgatg atcatagcaa gccgatacat tttgaataat
      181 ttatccaatt gaggtctcaa ccaaaagtac agctttggta ggaggagagg tgcagaatat
      241 gcaaggataa cagccaaaac cattggcgaa acactgaatg tagcaatgaa cgacaaaatg
      301 ctgtattgca gagcaaggag tgtgccgcca tagatgccaa ggcggatgat cattgaatca
      361 gcgaattgtt cgctatccca tagtagcaag agataactga gaattgcata caaaagtaaa
      421 gggaagaatc cccaagaagc ctctggcaaa aggaagagat agagatagga tgtaaaccga
      481 ccatcctgcc actctggttt catcagttca atcagcgaga aattaaagat cggggcagcg
      541 acgacgaaaa gcccaaagag aatacgcttc caagtccgat ctagcaatgg cggaataata
      601 ttttccccgc tgggcgtttg agctatttct tccatgcatg gctcctatta cttgacctga
      661 ttcaccaaag cgtacacttc cttcttattc catccgcttt tttctgcaag gtccgcagag
      721 atctcttttg cagatttgcc agcatctaat tcatgcttga tgacttctat cagttgtgat
      781 tctgcccaca cggttctttc ttcttttttt cttccttcaa tgaccaacgt aaattccccg
      841 cgagcttctt ttgccttgaa atattcaata gccccactta tgtctccgcg ccaatattct
      901 tcgtagagtt tggtcatttc gcgggcaacg caaatgcggc ggttgccaag catggcaagc
      961 aggtcttcaa gcgagtcaac gaggcggtgc ggggtttcga ggaagatgag ggtgtattcc
     1021 aagcctgaga cttcgcctgc aaacccacgg cgttcgctgg atttatgcgg aagataaccg
     1081 agatataaaa aagaatccgt gggcagacca gatacggtca aggcggagat gggggctgag
     1141 ggaccaggaa caggtcgaac gtcgaagcct gaggcgaggg cggcttgcac gagttcatag
     1201 ccagggtcgt taatggcggg cgtccctgca tctgagacga gggctacgtc gccttgtgct
     1261 aatttttcaa gaatgaaatc ttgcttggtg agtttattat gttcgaaata actggtgaga
     1321 ggtgtttcaa cattgaagtg cttgagcaag gtgccggtgt gacgtgtatc ttcagcggca
     1381 atcagaattg cttcgcgaag aatccgcact gcgcgcggac tcatatcttc aagatttcca
     1441 attggagtgg cgacaagata taaagttccc atgcaaataa ttatctcacg aaataaaaat
     1501 cgggcgattg ctcgcccgat tttgtttatc cgccgcagcc cttaagctgg atctttatta
     1561 ctttacatga tgctttttgg ggcggctgtt gaacacagca ttgattggct gcattgtagg
     1621 tgctgtctgc tgagcattga gaaagttctt caggttttaa ttcaacgggt tcacagactt
     1681 caaggtcgat cactgcgaaa ctgggaccag tacacgaaac gaccttttcg ccgttcttaa
     1741 tcgcttcctg acgacatttc gcaccagctg tgctggaatc taatattgta aacgttgcat
     1801 gatctgccat attggcatag gcatacggaa tcttctgtgt acagaaacta ttggtttctt
     1861 tcccaatagg tgcagggaag gaaacattgg cagaacaagc gggtgccgag gcgggaccag
     1921 aagctaatgt cgcttcgggc agggtagcgg tgactacaac caagaccggt gttgaagttt
     1981 cactgacttc tgttgaggct gcatctgttg tagaggttgg cgagctggat gcaaatagct
     2041 gattgcggag cagaaatact ccaacgatgg ctaaaagcag aatcacaccg ccgactgcca
     2101 agccagtggt ttgcccgcgc gataagggct ttgaattgga aggcagacgt gtacgtccat
     2161 tcatggtgtg gaaggtaata tcgcccggcg taccaaatgc cacggtattc aacgcctttg
     2221 ccaattcaat ggtgttggcg taacgttgag tcttgtcttt tgccatcgct gttttgatga
     2281 tggcatctac atcttcaggg agattgggaa gtactttaag aatttcgggc accggctcgg
     2341 tgatgtgctt gacgaccaca cccatggggg tatcggcgct gtaaggctga tgtccgctca
     2401 acatttggta gacgatcacg cccaagccat acacatcact gcgttgatcg atttcattcc
     2461 cctgagcctg ttcaggactc atataggcag gagtaccgat cacaccgcta ccggttagat
     2521 tactgcttga ctcagataat ttggcaacac caaagtcgga gatgaacggc tcgccatcgt
     2581 cattaaagag aatattgtct ggcttcagat cgcgatggat aacacctttg cgatgggcat
     2641 atgccaagcc ctgagcgatc ttttcgatga tcttcgctgt ctcatcaatc ggaatactgc
     2701 ctttttcgat gagatcggaa agggagccgc ccgtcatgaa gcgcatgaca aaataaggct
     2761 gtccatcaat atcgcctaca tcatacacag gcacaatggc tggatgttcc aatgaggcga
     2821 tcattttgat ctcgcgttca aaacgttcgc ggaattgcgg gtcgtgcatc atttcacgcg
     2881 gcaagacctt aacggcaacc tcgcgccccg agttggggtc aacggcgcgg tagaccgtag
     2941 ccataccgcc gcgtccgagt tctgatttaa cttcgtatcg accgattttt tctggaagtg
     3001 gcatagttca agtataaacc agccaaaaca ggggcgcaag aagattcacc catttgtaat
     3061 aaaaagacaa ctgccatttg gcagttgtct ttttattgat ttattactct atggctagtt
     3121 catactcgcc agattcttct tcgaccgcca tcacaggctc ttcacttgct tttcgatcga
     3181 ggaattgcag gctttgggca atgatcttgg tgtagtacct tgtttcgccg ctttcccgat
     3241 cttcgtactt atcggttttc aagcgacctt ctacgaagat caagctgcct tttttcaaat
     3301 attcttggca aacttcgccc agccgtcccc atgtttcgat gttgacccat tcggtggttt
     3361 ctttggtctc gccgttcttg tctttccaac ggttgctaac ggcgaggctg aagtgacaga
     3421 ccttcttgcc agtgggggtg tatttacttt ctgggtcttt ccccaaacga ccgatcaact
     3481 gaacgcggtt taatgctggc atggctgcac ccctttggtt tgagtggctt ttagatgctg
     3541 tgtacgtgtc ataattagtc tcctttttat ctaaaagaac aggttgatat tttcccggca
     3601 aaagccaggt tgtcgaacag ggttatactt gaattaccct gcactttccg tgttggtggt
     3661 ttctgtggtg gtggcgctgc cttctgcaac tgccttcttg aggacgggca tgccggtctt
     3721 catttcgacc acattgtaac cagcgggtac ggcatcgaga gcgccttcct taacctcttt
     3781 agcgaagaag aacatcttgc cgcgagcgtg aaggtagtag gtagttcctt ttgagttggt
     3841 gtatccgaaa gccattgtat ttctcctttt gtctaaattc tgataaaagt gtagaacaaa
     3901 atttctagcc tgtcaagtag tacttttgta aatgtataga caaattggag cctgacttga
     3961 aaattgtctc ctctctctct gacctaagag ctgctcgcct ctctctcgac gggacggttg
     4021 gtcttgttcc cacgatgggg tatcttcacg aggggcattt atcgcttgtc tctcgtgcca
     4081 aagccgagaa ccagcatgcc atcgtcacta tttttgtgaa ccccacccag ttcggaccga
     4141 acgaagacct ctcgaagtat cctcgtgacc tcgaacgtga cttagacctt ctgcgccccc
     4201 tcggtgtgga cgtggtttgg acgcccacgg cggaggtaat gtatccg
//
LOCUS       DAOQNJ010000187         4244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13221_length_4244_cov_7.685844, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000187 DAOQNJ010000000
VERSION     DAOQNJ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13221_length_4244_cov_7.685844"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..626
                     /locus_tag="PLG52_06675"
     CDS             18..626
                     /locus_tag="PLG52_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41165.1"
                     /translation="MKLSSRDRTLLLLTTLLASYQVSAGIDGFTALPITAYTIAFGVI
                     LVAALLIFILGMEVLDMPIVVIVSTIIPLSLSVGLVWQHLASWRTSYLVFALIGFLAV
                     TLTRSIQMQNKLPVVILAVTHGVAGLTIFFLPIILAIQTQVPPLFALVGLGGGLIGIV
                     GLLLSFQKTGLQIVSKENLMKFFPTFLLLTTALFVAGFKFGG"
     gene            complement(693..1175)
                     /locus_tag="PLG52_06680"
     CDS             complement(693..1175)
                     /locus_tag="PLG52_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41166.1"
                     /translation="MDILQLIDRLEELFNDAKAVPFTHNVVVDEDKMLELIDQMRIAI
                     PEEVKKAQQLVAQRDRVMAQAQEEANRTLQIARDKADGLVQKDMIVQEAQRRADQILA
                     QARAEAEGVRADADNYVLDTLMQLQDQIAKLSNQVNNGIRMVQEDQMRKVTSVGSDKE
                     "
     gene            complement(1218..1736)
                     /gene="coaD"
                     /locus_tag="PLG52_06685"
     CDS             complement(1218..1736)
                     /gene="coaD"
                     /locus_tag="PLG52_06685"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNB41167.1"
                     /translation="MVRALFPGTFDPIHYGHMDIANRASKLFDEVVMAVYDKPLKNLL
                     FSPEERMSLVNEAFKDNPKIKVTGYSGLTIEFAKKIDAQVIVRGLRVFSDFEFEFRMA
                     LANQRLAGAAVETVALITAEQHTFLSSSTVREIAMLQGNVSSMVPPHVERALKEKVAQ
                     MGEQSLPNALRD"
     gene            complement(1840..>4244)
                     /gene="recG"
                     /locus_tag="PLG52_06690"
     CDS             complement(1840..>4244)
                     /gene="recG"
                     /locus_tag="PLG52_06690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNB41168.1"
                     /translation="VSRLRAYEKLNPTGRADSLKGLWKRISETYPEAGQKPKAPPPPQ
                     RPNPQPQRPQRSDEEAKPKADVAEQLAVQNQSQPKQHPQQHQQKQQRPNNSPQRFESA
                     TSAKHSQTPAALGAKLTVLNGVGPKNAESLAKLGMQTLGDMLYSFPRRYEDYSLLKPI
                     QDLMYNDVVTVLGTIQSVHNRPIRGGKQSIIEVIISDGTGSLRVSFFNQPWLANRFKT
                     GDAISVSGKIDQYLGRIVMNSPDWENVEMESLSTNRIVPIYSLTERITQKWLRNQMKQ
                     VVTFWAPAVVDALPESIKHDAQLVSLGEALTQVHFPDTQDRLKKARERLAFDEIFYLQ
                     MGVLRQKRDWQVVEGKRFTALDEWLGARLAALPFTLTSAQQEALNDIRGDLDSGKPMN
                     RLIQGDVGSGKTVIAAMGASIVVSTGAQAAIMAPTSILAEQHYRNFSNLLTGENGFLQ
                     KEEVRLLVGNTSEAEKEAIRQGLADGSIKIVIGTHAVIEPDVTFKELQFIVIDEQHRF
                     GVEQRAELRSKGTNPHLLVMTATPIPRSLALTVFGDLDISVMNVMPVGRQPIATYVLR
                     PQERERAYTMIRSQVKNGNQAFIVYPLIEESEKINVRAAVDDFETLSTQVFPDLKLGL
                     LHGRMKPSEKDEVMLKFRDKEFDILVSTTVIEVGVDVPNSTLMLIEGADRFGLAQLHQ
                     LRGRVGRGSVASTCLLIPTHEDATENERLQAMASTNDGFELADLDLKLRGPGEFLGTR
                     QAGFASSLKMASITDVVLIEKAREQAKSLFERDPDLSQPEHALLAEAFERFWMGTSSD
                     VS"
BASE COUNT         1002 a         1125 c          964 g         1153 t
ORIGIN      
        1 ttcttgaagt tatctaaatg aaactttcct cacgcgatcg cactttattg ttactcacca
       61 ctctacttgc ctcttatcaa gtctctgcag gtatcgacgg tttcaccgca ctccccatca
      121 ctgcttacac cattgccttc ggggtcatcc tcgttgctgc gttattgatc ttcatcttag
      181 ggatggaggt gctggacatg cccatcgtgg tcatcgtctc aaccatcatt cctcttagcc
      241 tatctgttgg gctcgtttgg cagcacctcg cctcctggcg gacctcgtac ctggtcttcg
      301 ccttgatcgg tttcctcgct gttactttaa ctcgctctat tcaaatgcaa aacaaacttc
      361 cggttgtgat ccttgccgtc acgcatgggg tcgcagggct tacgatcttc ttcttgccga
      421 tcattctggc aatacagaca caggttccac ctctctttgc actagttggg ctcggcggcg
      481 ggttgatcgg tatcgttggg cttttgttat cgtttcaaaa gacgggtcta caaatagtat
      541 ccaaagagaa tctgatgaag ttcttcccca ctttcctgct tctcactaca gctctctttg
      601 tagcaggttt caaatttggc ggttaagctc aaagtccaga aagaagcgca taaataaaaa
      661 catccccgtc aaactgacgg ggatgttcgt ttctattctt tatccgaacc gacagaagtc
      721 accttccgca tttggtcctc ttgcaccatg cgaatgccgt tgttgacctg attactaagt
      781 ttggcgatct ggtcttgcaa ctgcatcagc gtatctagaa cgtagttatc tgcatctgcg
      841 cgaacgcctt ctgcttcagc gcgtgcctgt gccaaaattt ggtcagctcg gcgttgagct
      901 tcttgtacga tcatatcctt ttgcacaagc ccatcggctt tatcgcgggc gatctgtaag
      961 gtgcggttgg cttcctcttg tgcttgcgcc atcacacggt cgcgttgagc gacgagttgc
     1021 tgcgctttct tcacttcttc aggaatggca atacgcatct ggtcaatgag ttcaagcatt
     1081 ttgtcttcgt ctacaacgac attatgcgtg aaaggcactg ctttcgcatc attgaagagc
     1141 tcttccaaac ggtcgattaa ttgaagaatg tccataggtt actcacaaaa ggaaacgatt
     1201 ttcataattc tagcacatta atctcgcaaa gcattcggca gagactgttc ccccatttgg
     1261 gcaaccttct ccttcaaagc acgttccaca tgcggcggca ccatgctcga gacattacct
     1321 tggagcatgg caatctcccg caccgtagag gatgagagga aagtatgttg ctcggctgta
     1381 atgagtgcta cagtctctac ggcagcacca gcaaggcgct gatttgccaa cgccatccgg
     1441 aactcaaatt cgaaatcaga gaatacacga agcccgcgta cgatgacttg tgcgtcgatc
     1501 tttttcgcaa attcaatggt cagcccgcta tacccggtga ctttgatctt tgggttatct
     1561 ttgaacgcct cgttgaccaa agacatgcgt tcttcgggag agaacaacaa attcttgagc
     1621 ggcttatcat agaccgccat caccacttca tcgaagagct tagaagcgcg attggcaata
     1681 tccatatgtc cgtagtggat ggggtcgaac gttccgggaa ataaggctct aaccattgta
     1741 gtcttgtgat cctttagtga tttagtcttg tagtcgggtg gtcgattggt tgcgtcgtct
     1801 catagtgtgc atttgccctt caactacgaa actattcaac taactcacgt cgctcgacgt
     1861 tcccatccag aaccgttcaa aagcttcagc caacaaagca tgctcgggtt gagaaaggtc
     1921 tgggtcacgt tcgaaaagag acttggcttg ctctcttgct ttttcaatca gcacaacatc
     1981 ggtaatactc gccatcttca aggaagatgc aaaccctgcc tggcgcgtcc caaggaattc
     2041 accaggacca cgtaatttga ggtctaagtc agccaattca aaaccatcat tggtactcgc
     2101 catcgcctgt agacgttcat tttcagtcgc atcttcgtgc gtgggaataa gcaaacaagt
     2161 agacgccacc gaaccgcgac cgacacgtcc gcgtagctga tgaagctgag ccaaaccgaa
     2221 acgatctgcg ccttcgatca acatcaaggt cgagttgggc acatccacgc ctacttcgat
     2281 aaccgtcgtc gaaacgagaa tatcaaattc cttatcgcgg aacttgagca tcacttcatc
     2341 tttttcgctg ggcttcatcc gtccgtgcaa gagaccaagc ttgaggtcag gaaacacctg
     2401 tgtagacaat gtttcgaaat catccaccgc cgcgcggacg ttgatcttct cgctctcttc
     2461 aatgagcggg tacacaatga acgcctgatt gccatttttc acctgcgagc gaatcatcgt
     2521 atatgcacgt tcgcgttcct gcgggcggag aacataggtt gcaataggct gcctacccac
     2581 cggcatcaca ttcatcactg aaatatcaag gtcgccaaag accgtcaacg ccagtgagcg
     2641 cggaattgga gtagccgtca tcaccaaaag gtgcggattg gtacctttgc tgcgcaactc
     2701 agcgcgctgt tctacaccaa aacgatgttg ttcgtcgatc acaataaact gcaattcttt
     2761 gaaggtcaca tccggttcaa tgaccgcatg ggtgccaatc acaatcttga tcgagccatc
     2821 ggcaagcccc tggcggatcg cttctttctc cgcttcagaa gtattcccca ccaagaggcg
     2881 aacctcttct ttttgtaaga agccattttc gcctgttagt aagttcgaga agttacgata
     2941 atgctgttct gccaaaatag aagtcggcgc cataatggca gcttgggctc ctgtagaaac
     3001 cacgatgctc gcgcccattg ctgcaatgac cgtcttcccg gaacccacat ccccttgaat
     3061 gagtcgattc atcggtttac ccgagtctaa atcaccgcga atatcattga gtgcttcttg
     3121 ctgggcagag gtgagagtga aaggtaaagc cgctaaacgc gcacccagcc actcgtctaa
     3181 ggcggtgaag cgcttgcctt ccaccacttg ccagtcacgc ttttgtcgca acacgcccat
     3241 ttgcaaataa aaaatttcgt caaaggcaag gcgttcccgc gctttcttaa gccggtcttg
     3301 cgtatcagga aaatgaactt gtgtaagtgc ttcacccagt gaaaccaatt gtgcatcatg
     3361 tttgatgctt tctggtaacg catccaccac agcaggcgcc caaaacgtaa caacttgttt
     3421 catctgatta cgcaaccatt tttgagtgat gcgttcagtg agcgaataga tcggcacaat
     3481 acgattggta ctgagacttt ccatttcaac attttcccaa tcggggctgt tcattacaat
     3541 gcgaccaaga tactgatcga tctttccaga aacagaaatc gcatcgcccg tcttgaaacg
     3601 attcgccaac caaggttggt tgaagaacga gactcgcaat gagcctgtgc catcagaaat
     3661 gatgacttcg ataatgcttt gctttccacc gcgaatcgga cggttatgta cactttgaat
     3721 ggtgcctaac acagtcacca catcgttgta catcaaatct tgaatcggtt tcagcagtga
     3781 ataatcttca taccgacgcg ggaaggaata cagcatatct cccagcgttt gcattccaag
     3841 ctttgccaat gactcagcat tcttcggtcc caccccattc aagaccgtaa gtttggcacc
     3901 caacgcagca ggtgtttggc tatgcttggc actggtcgca gattcaaaac gttgcggaga
     3961 attatttggt ctctgctgtt tttgttggtg ttgttgagga tgctgcttcg gctgactctg
     4021 attctgcacc gcaagttgtt cagcgacatc cgcttttggc ttcgcctcct catcgcttcg
     4081 ctgcggacgt tgaggctgtg gattcggtct ctgcggcggt ggtggcgctt ttggcttttg
     4141 ccctgcttct gggtaagttt cgctaatgcg cttccacaag cccttaaggg aatccgcccg
     4201 cccggtggga ttcaactttt cataagcgcg taaacgggaa acaa
//
LOCUS       DAOQNJ010000188         4240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13238_length_4240_cov_7.038471, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000188 DAOQNJ010000000
VERSION     DAOQNJ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13238_length_4240_cov_7.038471"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2959
                     /locus_tag="PLG52_06695"
     CDS             <1..2959
                     /locus_tag="PLG52_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB41169.1"
                     /translation="TEKGPRHHNIRFVAERGDDGTITGVLAIGRDITERKQMEEALYA
                     SEAELRALISSMTDLIFVGNAEGRFLKVVDTRPKNFQKPSNDLVGKTLHEIFPKELAD
                     FFLAQLHKTLKEQTPVNFEYSLPVDGTTMWFYATVSPMSADKTLMVARDITELKLVED
                     ALRQSREATLHFSKQLTALQEVINQLSEAESADDLNRMAVEIGRNLLEVDRMGIWFVD
                     GQKKLMHGSFGIDEQGNLRDERNSTIELKEEGLAWLLLSTKKPMAQIENHALQDDKNR
                     KVGEGYSAQAALWDGNNVLGIVSVDNLLSGMPISEQQLDILQLYANSLGYLIKRKWAE
                     ERLLSSEKKYRALAENIPNVVFQCKNDERYTFVYLNKSVLELTGYSKEDFLEKGLSFF
                     DLYHPEDRVNIPKPSTTNLSLINHRPYHLSYRIKHRSGEWRWVDEWGTGVINEKDEVE
                     YIQGIMIDITSRKQYERERETIITVSAALRQATNRNEVLGTILNQMIELLDAGGAFIA
                     IPDGHNSNVLIEMGRGDIGSRYHGMQIPQGRGASGWVIAHRFPYLNNQAQLDPIFYRP
                     DMLGEYHCVAAVPLIAHDQSIGALWLARKTEITEQDLKLLSAIADIAANAIHRITLHE
                     QTEQQLNHLLALHQIDLAITTNFNLEDTLTIILGHVRTELNVDAASILLMNEAENTLE
                     YAAGLGFKTEHIKLTHVQVGDGTAGRAALEKSTASFLNLKLISPENNRYSLIMDERFT
                     SHVATPLLLKGQVKGVLEVFHQTSLNPDSKWFHYFETLGAQTMIALENANLLKTLQLK
                     NEELTLAYDATIEGWSRALDLRDHETEGHTQRVTEMALKLADLLGMSQTDKTDMRRGA
                     LLHDIGKMGVPDAILHKPGDLTETEWKVMREHPTLAYRMLSPISYLNRALDISYYHHE
                     RWDGSGYPHGLKGEEIPLAARVFAIVDVFDALTSDRPYRKAWTYEKAYQYIELQAGKH
                     FDPEIVKVFLTNK"
     gene            2987..3712
                     /locus_tag="PLG52_06700"
     CDS             2987..3712
                     /locus_tag="PLG52_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB41170.1"
                     /translation="MPSVQLFITCLTDSFFPETGEAIVDIFSRLGIRVDFTPEQTCCG
                     QPQFNAGLRTDARAIAEHTINVFEKTSGDIVTPSGSCAHHFRHNLLELFEGDAEWYPR
                     AEKFGKRVFEFTEYLVDRLGVTDLGAAWDGLLTYHPSCHTLRGLNVDKQPRTLLQHVK
                     GATLVELPNAHECCGFGGVMSVEHPELSAEWLKRKISNLQSTNSPTLVVTDAGCLMHI
                     MGGLKRQGKSQRVMHIAEILRNT"
     gene            3733..>4240
                     /locus_tag="PLG52_06705"
     CDS             3733..>4240
                     /locus_tag="PLG52_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNB41171.1"
                     /translation="MTTFRNRIRQSIENPTLQTALDNNTERRLKGKALAFESIPDWRE
                     RRQRAHRIRAEVIKHLEQYLDQFITNAQANGVIVHRAKDAAEAIDIVLKITQSITNYQ
                     LPNALIAKSKSMVSEEIELNHVLEKHGINVVETDLGEYIVQLRKEKPSHIITPAAHLK
                     KEQVAQLFH"
BASE COUNT         1240 a         1052 c          955 g          993 t
ORIGIN      
        1 tacagaaaaa ggtccccgcc atcataatat ccgtttcgtg gctgaacgtg gagatgatgg
       61 aacgatcacc ggggtacttg ccataggtcg cgatattaca gaacgcaagc aaatggaaga
      121 agcgttatat gcctcggaag cagagttgcg agccttgata agttcaatga cagaccttat
      181 ttttgtagga aatgcagaag gtcgtttctt gaaagtcgtt gatacccgtc ctaagaattt
      241 tcaaaaacca tcaaatgatt tagtggggaa aacattacat gaaattttcc ccaaggaact
      301 cgctgacttt ttccttgctc agctgcacaa aacgcttaaa gaacaaacgc ctgtgaattt
      361 tgaatacagt cttccggttg atggcacaac aatgtggttt tatgccacgg tctcgcccat
      421 gtctgcggat aaaacgctga tggtagcacg ggatatcaca gaactaaagc tggtagagga
      481 tgcactacgt cagagtcgtg aagcgacctt gcatttttcc aaacaactga cggctttgca
      541 agaggtcata aaccagcttt ctgaagcgga gtctgcggat gatctaaacc gtatggcagt
      601 ggaaataggg cgcaatttac tggaagttga ccgtatgggc atctggttcg ttgatggaca
      661 aaaaaaactc atgcacggtt ccttcgggat cgatgaacaa ggcaacctac gcgatgaacg
      721 caattccaca atagaactaa aagaagaagg gttggcttgg cttttgctat ccaccaagaa
      781 acctatggca cagatcgaaa atcatgcctt acaggatgac aaaaatcgta aagtaggcga
      841 aggctatagt gcccaagctg cgttatggga tggtaacaat gtactcggta tcgtcagtgt
      901 tgataacctc ttatcgggta tgccaatttc ggagcagcag ctcgacatat tgcaactata
      961 tgcaaactcc ctgggctatc tcataaagag gaagtgggct gaagagaggc tgcttagcag
     1021 tgaaaaaaaa taccgggcat tggcagaaaa tatcccaaat gttgtatttc aatgcaaaaa
     1081 cgatgaaaga tatacgtttg tttatttaaa taaatcagtt cttgaactta caggttattc
     1141 taaagaagat tttcttgaaa aaggattaag cttttttgac ctttatcacc ctgaagatcg
     1201 agtaaacatt ccaaaaccct caacaacgaa tctgtctctt ataaaccatc gtccatatca
     1261 tctttcctat cgtataaaac accgttcagg cgaatggcgc tgggtggacg aatggggcac
     1321 aggcgttata aatgaaaaag atgaggttga atacattcaa gggatcatga ttgatatcac
     1381 cagtcgcaaa caatatgaac gtgaacgcga aacgatcatt accgtcagtg cagctttgcg
     1441 gcaggcaacc aatcgcaatg aggttctggg caccattctt aaccagatga tagaactact
     1501 cgatgctggc ggggcattta tcgccatacc cgacggtcac aatagcaacg ttctaatcga
     1561 aatggggcgc ggagacattg gcagtagata tcatggcatg cagatcccac aagggcgcgg
     1621 cgccagtggc tgggtcattg ctcaccggtt tccatatttg aacaatcagg cgcaattaga
     1681 tcccatcttt tatcgacctg atatgttggg cgaataccac tgcgtcgctg cggtgccctt
     1741 aatcgcgcac gaccagtcga ttggtgcatt atggcttgca agaaaaacag agatcaccga
     1801 acaagacctg aagttgctct ccgccattgc agatattgcc gccaacgcca ttcatcgcat
     1861 cacacttcac gaacaaaccg aacagcaact taatcacctg ctcgcactcc atcagatcga
     1921 ccttgcgatc acaaccaact tcaatcttga agatacactc actattattc ttggtcatgt
     1981 tcgcaccgaa ctcaatgtgg atgccgccag tatccttctg atgaatgaag cggaaaatac
     2041 tttggaatat gctgcagggc ttgggtttaa gacagagcat attaaactca ctcatgtaca
     2101 ggtcggggat ggaaccgctg gtcgtgctgc acttgaaaaa tccaccgcct cttttctaaa
     2161 cctcaaactt atcagccctg aaaacaaccg atactcgctg ataatggatg aaaggttcac
     2221 atcgcacgtt gcaacccccc tgctccttaa gggacaggtc aaaggtgttt tagaagtgtt
     2281 ccaccaaacc tctctaaacc cagatagcaa gtggtttcat tactttgaaa ctcttggtgc
     2341 tcaaacaatg atcgccctcg agaatgcaaa cttattaaag acattacaac ttaagaacga
     2401 agaattgact cttgcctatg acgccactat agaaggctgg tcacgtgcat tggatctacg
     2461 cgaccatgaa acagaaggtc acacccaacg ggttactgaa atggctctca aactggcaga
     2521 cctgctgggg atgagtcaaa cagacaaaac cgatatgcgg cgcggcgcgc ttttacatga
     2581 cattggcaaa atgggtgtgc ccgatgcaat tctccacaag ccgggcgatc tcaccgaaac
     2641 cgaatggaag gttatgcgtg aacacccaac gctggcgtat cgcatgttat cgccgatctc
     2701 atacctaaac cgcgcattag acatttccta ctaccatcat gaaagatggg atggaagcgg
     2761 atacccacat ggattaaagg gtgaggaaat tccgctagcc gctcgcgtct ttgctatcgt
     2821 agatgtattc gatgctttga cctccgaccg cccctatcgc aaggcatgga catacgagaa
     2881 ggcatatcaa tacatcgaat tacaagctgg caaacatttt gatcctgaaa tcgtaaaggt
     2941 ttttctgaca aataaataga acacatagaa gtcgtataat cgacgcatgc ccagcgtcca
     3001 acttttcatc acctgtctca ccgattcatt cttccccgaa actggtgaag ccatcgtgga
     3061 cattttctcc cgcctcggca ttcgtgtgga cttcaccccc gagcagacct gctgcgggca
     3121 gccgcaattc aacgccggtc tgcgaacgga cgcgcgcgcc atcgcagaac acacgatcaa
     3181 tgtgttcgag aaaaccagcg gagacatcgt cacgccttcc ggctcttgtg cgcatcattt
     3241 caggcataat ctcttggaac tgtttgaagg ggatgcggag tggtatccgc gtgcggagaa
     3301 gtttggcaag cgcgtttttg aattcaccga atatctcgtg gatagacttg gagtaacaga
     3361 ccttggtgct gcatgggacg ggctactcac ctatcacccc tcctgccaca ccctgcgcgg
     3421 actcaatgta gataaacagc cacgcacgct tttacaacat gtaaaaggcg caacgctggt
     3481 cgaattaccc aacgcgcacg aatgctgcgg cttcggcggc gtgatgtccg tcgagcaccc
     3541 cgagttatcg gcggagtggc tgaagcgcaa aatctccaat ctccaatcca ccaattctcc
     3601 aaccttggtc gtcaccgatg cgggctgcct catgcatatc atgggcgggc taaagcgaca
     3661 gggcaaatca caacgtgtaa tgcacatcgc cgaaattctt cgaaacacat aacttataaa
     3721 aacctgctac tcatgaccac tttccgcaac cgcattcgcc aatccattga aaacccaact
     3781 ctccaaactg cgctagataa caacaccgag cgtaggctca aaggcaaagc ccttgcattc
     3841 gaatccatcc ctgattggag agagagacgt caacgcgcgc atcgcatccg tgccgaggtc
     3901 attaagcatc tggagcaata tcttgatcaa tttatcacca atgcccaagc gaatggagtc
     3961 atcgttcacc gtgccaaaga tgcagccgaa gccattgata ttgttctgaa aatcacccaa
     4021 tcaattacca attaccaatt accaaacgcc cttatcgcca aatccaaaag catggtcagc
     4081 gaagagatcg aactgaatca tgtgctcgaa aaacatggca tcaacgtcgt cgaaaccgat
     4141 ttgggtgaat acattgtgca actccgcaaa gagaagccga gtcatatcat caccccggcg
     4201 gcacacctca aaaaagaaca ggttgcgcag ctctttcatg
//
LOCUS       DAOQNJ010000189         4226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13304_length_4226_cov_8.216495, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000189 DAOQNJ010000000
VERSION     DAOQNJ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13304_length_4226_cov_8.216495"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..845
                     /locus_tag="PLG52_06710"
     CDS             24..845
                     /locus_tag="PLG52_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNB41172.1"
                     /translation="MQLTDTHCHLDYHKYNTDRADVIQRAKDAGLVKVLVPGLHHRSS
                     HEAVKLAESNTMVYAAVGFHPTDLDEFSEKAFLQVKELAKHPKVVAIGEIGIDYYWVK
                     EPEKRAVQREVLKQHLAFAQEVNKPVVLHMREQDDAWFGEASVDLLEILSEWQNGLRG
                     ELREKPGVLHSFNGTLETAQKALSLNFYLGVTGPVTYPSAEKKREVISQLPLDRLLIE
                     TDGPYLTPAPHRGKRNEPAYVVHIADKIAEIHSKSPAEIAQITTANAKRLFAWGD"
     gene            847..1812
                     /locus_tag="PLG52_06715"
     CDS             847..1812
                     /locus_tag="PLG52_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNB41173.1"
                     /translation="MNSVTFLSPVTEEIKLIEDRMRIQADDSHPDLRAALKHLLAAGG
                     KRIRPTLGMLVGNMLGAPLEKLINLGAAVELLHTATLVHDDLIDGSLLRRGTPTLNAR
                     WSPPATVLTGDFLFARAAKLAADTDYLPLMKLFSETLAIIVNGELTQMFTSRGLINRD
                     NYYKRIYAKTASLFEMTSRSAAMVSPASDDSIEAMRDFGYQIGMAFQIVDDILDFNGD
                     QNAVGKPLGSDLLNGLVTLPAIYFAEINPDDPDVMSLPQGGWTNNENMTRLVEKIRAS
                     SCTKKAMVEAEQHVDRALVCLESFEPCAERDALENLAKYIVDRKV"
     gene            1879..2388
                     /locus_tag="PLG52_06720"
     CDS             1879..2388
                     /locus_tag="PLG52_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNB41174.1"
                     /translation="MTNPRKPFRFNVGFIIHEEVGYNHDFPFEFEKVTLNGDLELRNL
                     EGNVNVGKTPQGLIVQGKFTAETTVTCSRCLTDVVQDLEWEFTEMYAFDQRSITESGL
                     LLPEDAQLDVESLLREYALLEVPINPICKPDCLGLCIECGQNLNEKDCGHRPEEPDSP
                     FAKLKDLLK"
     gene            2432..3634
                     /locus_tag="PLG52_06725"
     CDS             2432..3634
                     /locus_tag="PLG52_06725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNB41175.1"
                     /translation="MKLSIPKPIRILITILVLVVLIFIRVRINLGFDVPNSNFSFFWL
                     AGRMILDGQNPYDETQYLAGHDANSMDWRPNNIFPYPLPLALFCIPLGFFSMQTAYII
                     WQIVTLLLIALTIYLLLQHWQEESQQNLLLPIFFFLLFLGPVYLSTHAGTISILPVIV
                     LVGAILLVEKEQSLWAGVVLALTMLKPPQGLTILILAGVWFLARRDWKAIIGVALGGL
                     AILVVGLIQDPQWIQKFLGAGDAVMARTLGVHSNVWAFAYLACKGAEPCSNLLGGALS
                     LSLLGGASLLLWKNQAKWSAWEAMNLIIPIGFVSTIYLWAYDQLPYIIPIVWIVATLA
                     QKQRSILLAFFFLILLDLASIFALVQQAFTEKDLWSLGTTLVVLLFLFVAWRMKPKPA
                     IDKAPASA"
     gene            3860..>4226
                     /locus_tag="PLG52_06730"
     CDS             3860..>4226
                     /locus_tag="PLG52_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB41176.1"
                     /translation="MTETILVAVAWPYANAEIHVGNITGSHLPGDIFARYNRLKGNKV
                     LMITGTDSHGTPVTMSADKLGKPVEEVYKSFHEGFIELFQQIGITYDLYTTTHSDNHF
                     KVSQAMFLALQKNGYLYREF"
BASE COUNT         1046 a         1109 c          997 g         1074 t
ORIGIN      
        1 cagactgata taatgccaac gctatgcaat tgaccgacac ccactgtcac cttgattatc
       61 ataaatacaa cacagaccgc gcagatgtaa tccaacgtgc aaaagatgca gggttggtaa
      121 aggttctggt accaggactc catcacaggt ccagccatga agcggtgaag ttggcagagt
      181 ccaacacaat ggtgtatgcg gcagtcggct tccatcctac agatttggac gagttttctg
      241 aaaaagcgtt cttacaagtg aaggaattgg cgaagcaccc caaagtggtt gccattggcg
      301 agatcggcat tgactactat tgggtcaaag agccagaaaa acgcgccgtt caacgcgaag
      361 ttctcaaaca acatttagcc tttgcgcaag aagtgaacaa acccgtggtt cttcacatgc
      421 gcgaacaaga cgacgcctgg tttggcgaag cctctgtgga tttgttagag atcttgagcg
      481 agtggcaaaa cgggctacgc ggagaattgc gcgaaaaacc cggcgtttta cactcgttca
      541 atggcacgtt agagaccgcc caaaaggcgc tgtcactgaa tttttacctc ggtgttacgg
      601 gtccagtgac atacccaagc gcagagaaaa aacgcgaggt gatttctcaa cttccgctcg
      661 atcggctgct cattgaaaca gacggtcctt accttacacc tgccccacac aggggtaaaa
      721 gaaacgagcc cgcctacgtc gttcatattg ctgataaaat agcagaaatc cattccaaaa
      781 gccctgcgga gatcgcccaa attaccactg ccaatgccaa acggctcttc gcatggggag
      841 attgacttga actcggtaac ctttctttca cccgttacag aagaaataaa actcattgaa
      901 gatcgcatgc ggattcaggc agatgatagc caccctgacc tgcgtgccgc tttgaaacac
      961 ttattggcag ccggagggaa gcgcatccgc ccgaccttgg gcatgcttgt tggcaatatg
     1021 ctgggtgccc cactggaaaa gctcatcaac ctcggcgccg ccgtagaact tttgcacact
     1081 gccaccttag tccacgatga tttgatcgac ggctcgttac ttcgccgcgg cacgcctacc
     1141 ctgaacgcac gctggtctcc acctgcaacc gtgctcactg gcgactttct ctttgcccgc
     1201 gccgccaaac tcgccgcaga tacagactac ttacctctga tgaagttgtt ttctgaaacc
     1261 ttggcgatca tcgtcaatgg cgaattgacg caaatgttca catcacgtgg gctcatcaac
     1321 cgcgataact actacaagcg catttacgcc aaaaccgcct ccctcttcga aatgacctcc
     1381 cgctctgctg caatggtcag cccggcaagc gatgactcaa ttgaagccat gcgcgatttc
     1441 ggctatcaga tcgggatggc attccagatc gtggatgata ttttggactt caatggcgat
     1501 cagaatgctg tgggcaaacc gctcggttca gacctactga atggcttggt caccctccca
     1561 gccatttact tcgctgaaat caaccccgac gacccagatg tgatgtcgct gccccaaggc
     1621 ggttggacca ataacgaaaa catgacccgc ctcgtggaaa agatccgcgc cagtagctgc
     1681 accaagaaag ccatggttga agccgaacaa catgtagacc gggcactcgt ctgccttgaa
     1741 agttttgaac cgtgtgccga acgcgatgcc ttggaaaact tggcaaagta tattgttgat
     1801 agaaaagtct aagaatcagg aagcctgatt taacagaccc tgatttacta gtctcttatt
     1861 cctaatccct tatttcccat gaccaaccct cgcaaaccct ttcgttttaa cgtcggcttc
     1921 attattcatg aagaagtcgg ctataaccac gactttccgt tcgagttcga gaaggtcact
     1981 ctcaacggtg acttggaact ccgcaacctg gaaggcaatg tcaacgttgg caaaacccct
     2041 cagggattga tcgttcaagg caaattcacc gctgaaacca ccgtcacttg ttcacgttgt
     2101 ctcaccgacg ttgtacaaga ccttgaatgg gaattcaccg aaatgtacgc cttcgaccaa
     2161 cggtctatta cagagtctgg tttgctgttg cccgaagatg ctcaactaga tgtagagagc
     2221 cttctgcgcg agtatgcgct tttagaagtg cccatcaacc cgatttgcaa acccgattgt
     2281 ctcggtctgt gcattgaatg cggtcaaaac ctcaacgaga aagattgcgg tcaccgcccc
     2341 gaagaacctg attcgccttt tgcaaaattg aaggacttgc ttaagtaaaa cctgcatgcc
     2401 acctgcttac gcgggtggtt ttcatttttc tatgaaactt tccattccta agcctattcg
     2461 catcctcatc acaatcctag ttctggtagt tttgatcttc atccgcgttc ggattaacct
     2521 cgggttcgat gtccctaact ccaatttttc tttcttctgg ttagcagggc ggatgatttt
     2581 ggatggacaa aacccatacg acgagactca atatctcgca gggcacgatg ctaacagcat
     2641 ggattggcgc ccaaataata ttttccctta tccactaccc ttggctttat tctgtattcc
     2701 gctcggcttc ttttccatgc agacggcgta catcatttgg cagattgtta cgcttttgct
     2761 gattgccctg accatttacc ttctgttgca acactggcaa gaggaaagtc agcagaattt
     2821 attgctgcct atatttttct tcttactctt tttaggtccg gtctacctga gcactcatgc
     2881 agggacgatc agcatcctac ctgtgatagt gttggtaggt gcaattctct tagtggaaaa
     2941 agaacaatct ttatgggcgg gtgtcgtcct tgcgttgaca atgctaaaac cgcctcaagg
     3001 gcttaccatt ttgatccttg ccggggtatg gttccttgca cggcgtgact ggaaagcaat
     3061 catcggcgtg gcgcttggcg ggcttgcgat tctcgtggtg ggtttgatcc aagacccgca
     3121 gtggattcaa aaattcctcg gcgcaggcga tgccgttatg gcgcggactc tcggagtgca
     3181 ctcgaacgtt tgggcgtttg cctatctcgc ctgcaaggga gcggagccat gctcaaatct
     3241 attgggcggg gcattgagtt tgagtctgct tggaggggcg agtttgctcc tttggaagaa
     3301 tcaagccaaa tggtcggcat gggaagcgat gaacttaatc atcccgattg ggtttgtgag
     3361 cacaatttat ctctgggcgt atgaccagct tccatacatc atcccaattg tttggatcgt
     3421 ggctacattg gcgcaaaagc agcgtagtat tttgctagca ttcttctttt tgattttgct
     3481 ggatctggct tcgatctttg cgctcgttca gcaagctttt accgaaaagg acttgtggag
     3541 cctaggcacg actttggtcg ttctgctatt tttgttcgtg gcgtggcgca tgaaaccgaa
     3601 acctgccatt gacaaagcgc ccgcctctgc ctaaaataac ggacatgaaa ttcacttccg
     3661 ctttcccgat ggtgccaatg accatggcca tgcgcagcat gatgcgaatg gaaatgtccc
     3721 cttgcgccaa gtggaaagca tagtttttca tacctgaaaa aacgggctgt ccatttcgcg
     3781 gacagccttt ttgtttgaaa agaaccgata ttcgatcttc gaatatcgaa acccgaatat
     3841 cgaggaacgg agttccacca tgaccgaaac cattttagta gccgtcgcct ggccgtatgc
     3901 caatgccgaa attcacgtgg gcaatatcac aggttcccat ttgcccggcg atattttcgc
     3961 ccgttataac cgactcaagg gcaacaaggt gttaatgatc accggcaccg actcgcacgg
     4021 cacgcccgtc accatgagcg cggacaagct cggtaagccc gttgaggaag tgtataaatc
     4081 tttccatgaa ggcttcattg agttgttcca gcagatcggc attacctacg acctgtacac
     4141 caccactcac agtgacaatc acttcaaggt ctcgcaggcg atgttccttg ctttgcagaa
     4201 gaacggctat ctctaccgcg agttca
//
LOCUS       DAOQNJ010000190         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13545_length_4180_cov_9.299636, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000190 DAOQNJ010000000
VERSION     DAOQNJ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13545_length_4180_cov_9.299636"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLG52_06735"
     CDS             complement(<1..730)
                     /locus_tag="PLG52_06735"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41177.1"
                     /translation="MPQEYIEIRGARENNLKNVSLRIPKRKITIFTGVSGSGKSSIVF
                     DTIATESQRLLNENFSMFVRNFLPKYSQPEADSIENLSMSIVVDQKRMGGGSHSTVGT
                     ITDINTILRMMFSRLGQPHVGPTNVFGFNDPQGMCPNCNGLGRKLDVDMDKFLDKSKS
                     LNEGAILFHEYAVDSWYWSNLINTGLFDPDKKISKYSQKEMNDLLYSEPIKVKTKVGA
                     KDFNMTYEGIITRFTNKYIKQDLKT"
     gene            complement(855..1310)
                     /locus_tag="PLG52_06740"
     CDS             complement(855..1310)
                     /locus_tag="PLG52_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41178.1"
                     /translation="MSPVKKTTQKAEGFTADEKAAMKARALELKLEAKASKNREEGEK
                     ALLAAVAAMKDPTDRSMGKRIHELVTAAAPELMPKTWYGMPAYADADGKVICFFQAAS
                     KFGVRYATFGFQPDAKLDDGNMWAASFALIKLTSAEEAKITALVKKSVS"
     gene            1401..1868
                     /locus_tag="PLG52_06745"
     CDS             1401..1868
                     /locus_tag="PLG52_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB41179.1"
                     /translation="MSKSTKTDLKIRALMAVRDYGVNLTYFRNAMNEWAGLNATDMEC
                     LRLLSQKGTATPTELAKHTGLTSGATTAMLDRLEKAGLIERRPNPNDRRGTLIAPEAS
                     SSAKMASWFESARKAQDELMSTYSESELEIIADVFERFAKLWDAERKKVQKNQ"
     gene            complement(1890..2171)
                     /locus_tag="PLG52_06750"
     CDS             complement(1890..2171)
                     /locus_tag="PLG52_06750"
                     /inference="COORDINATES: protein motif:HMM:NF025482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HNB41180.1"
                     /translation="MSFQAYLDNVEEKTGKTPKQFIAEAKKKKLTEFKDLMNWLKTDY
                     GLGTGHARAIIHVIQHGDTFTVKQTTGTHRDASGKLKLDGKKKAKKRTA"
     gene            complement(2215..2811)
                     /locus_tag="PLG52_06755"
     CDS             complement(2215..2811)
                     /locus_tag="PLG52_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNB41181.1"
                     /translation="MRKIVVLEHISLDGVIQAPGGPEEDTSGGFTQGGWIANYGDDIS
                     GKLLRKQMNSPFDLLLGRKTFEIWEPYWPNHGDIWPNVNKATKYVASNSRTSSEWQPS
                     VFLSGDIAGEVAKLKQQPGPDLHVWGSGELLQTLMKHDLVDVFWLMLYPITLGSGKRL
                     FADGTLPAVFKVTESVATPSGVLVVSYERTGANTNGNL"
     gene            complement(2814..3272)
                     /locus_tag="PLG52_06760"
     CDS             complement(2814..3272)
                     /locus_tag="PLG52_06760"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB41182.1"
                     /translation="MSKITPFLWYDDPQAEEAANYYVSIFKNSKVLSVHRKPDGNAWT
                     VSFELDGVEYMALNGGPMFKFTEAFSMHVLCEDQAEVDYCWDKLSAIPEAESCGWCKD
                     KWGLSWQIVPKQLNDYIGGPDSVKAERGMEAMLQMKKLIIADLKKAYDGE"
     gene            complement(3274..3645)
                     /locus_tag="PLG52_06765"
     CDS             complement(3274..3645)
                     /locus_tag="PLG52_06765"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB41183.1"
                     /translation="MAKTNFQSIDEYIVACPPESQDYMQQIRKLIRSLVPDAKERISY
                     QMAAFERNGKNFIHFAGWKKHVSLYPVPAGSEAFEREIAKYVDGKGTVKFALDEPLPI
                     KLIERVVKLHLAVNKKKAKGD"
     gene            complement(3632..>4180)
                     /locus_tag="PLG52_06770"
     CDS             complement(3632..>4180)
                     /locus_tag="PLG52_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNB41184.1"
                     /translation="YLVLLRGINVGGKNIIKMADLKASFEEMGFSNVVTYIQSGNVLV
                     QSEDKDKAALTTKIEKGLSKRFNFVAKVVVISQKELAAIIKSAPDGFGEDEKKFRYDV
                     IFLKEPLTPKEAMESVKVREGVDMAHAGKQVLYFSRLISRASQSYLTKIIGMPVYQNM
                     TIRNWNTSTKLLALMEGQNGEN"
BASE COUNT         1003 a         1113 c          899 g         1165 t
ORIGIN      
        1 ttgtttttag atcctgtttg atgtacttgt tggtaaagcg agtgatgatg ccttcgtagg
       61 tcatgttgaa gtctttcgca cccactttgg tcttgacctt gatcggctcg ctatacaaca
      121 ggtcgttcat ttctttctga gaatatttgg aaattttctt atcggggtcg aacaaacccg
      181 tattaattaa attgctccaa taccagctat ccacagcata ttcgtggaat aagatcgcgc
      241 cttcgttcaa cgatttggat ttgtcgagga acttgtccat gtccacatcg agtttgcgcc
      301 caagcccgtt gcagttcggg cacatccctt gcgggtcgtt aaagccgaag acgttggtgg
      361 gtcccacatg gggttgaccg aggcgggaaa acatcatgcg taggatcgtg ttgatgtcgg
      421 tgatcgtgcc cacggtcgag tgtgagccgc cgcccatccg cttctgatcc accacaatgg
      481 acatgctcaa gttctcgatg gagtccgctt caggctggga atacttcggc aggaaattcc
      541 gcacgaacat gctgaagttt tcgttgagca ggcgctgtga ttcggtcgcg atcgtgtcga
      601 acacgatgga tgatttgccc gaaccggaca cgcctgtgaa aatggtgatc ttgcgtttgg
      661 ggatgcgcag cgaaacgttc tttaaattgt tctcgcgcgc gccgcgaatc tcaatatatt
      721 cctggggcat ggtctctcct ctattttctc tgccaaacaa aaacaaagcg ggcaggtaaa
      781 agcagggttg cttttaccca cccgtaagaa acgaactatg attttagaac attctttcta
      841 tatagtcaag gctcttaact caccgatttt ttcaccagcg ccgtgatctt cgcctcttca
      901 gcggatgtca actttatgag cgcaaagcta gccgcccaca tattgccatc gtccagcttc
      961 gcatccggct ggaagccgaa agttgcatac cgcaccccaa acttgctcgc cgcctgaaag
     1021 aaacaaatga ccttgccatc tgcatcagca taggcaggca tgccgtacca ggtcttgggc
     1081 atcagttccg gcgcagcggc cgtgactagt tcatggatcc gcttccccat cgaacgatcc
     1141 gttgggtctt tcatcgcggc aactgctgcc agcaaagctt tctcgccttc ttccctattc
     1201 ttgcttgctt tcgcttccaa cttcaattcc aatgcacgcg ctttcatcgc ggctttttcg
     1261 tcggcggtaa aaccttctgc tttttgtgtg gtcttcttaa cggggctcat ggaaatctcc
     1321 ttttctttac cttgccataa agtcaattta gttatataat aaagatactt tattataaag
     1381 ataatgtcaa gggatattcc atgtcaaaat caacaaagac cgatttgaaa atacgcgcct
     1441 tgatggctgt gagagattac ggggtgaacc tgacctactt ccgcaatgcc atgaacgaat
     1501 gggctgggct caacgccact gatatggaat gcctgaggct gctatcgcaa aaaggcactg
     1561 ccacccccac cgaactcgcc aaacatacgg ggctcacttc gggcgccacc actgccatgc
     1621 tcgaccgact tgaaaaagcg ggtttgatcg aacgccgacc caaccccaat gaccgccgtg
     1681 ggactctgat cgctcctgaa gcatcgagca gcgcaaagat ggcgtcatgg ttcgagtccg
     1741 cgcgcaaggc acaagacgag ttaatgtcaa catattcaga gagcgaactc gaaattatcg
     1801 cggatgtctt cgaacgcttt gcaaaacttt gggatgctga gcgtaaaaag gtccaaaaga
     1861 atcagtagaa caaatcagat cattttcttt caagcagttc gcttttttgc ttttttcttc
     1921 ccgtctaatt tcagcttacc cgatgcatcg cgatgtgtgc cagtcgtttg tttgaccgta
     1981 aacgtatcgc catgttgaat gacatggata atggctctgg catgtcctgt gccaagtccg
     2041 taatcggttt ttaaccagtt cattagatcc ttgaattcgg tcagcttttt ctttttcgcc
     2101 tcggcaatga actgcttcgg ggttttgcca gtcttttctt ccacattatc caaatacgct
     2161 tgaaatgaca tgttttactc ctatgcatgc agcaaacttt tcgggtttaa ggacttataa
     2221 attcccgttt gtgttcgcgc ctgtgcgttc ataactcact acaagaacac cactcggtgt
     2281 ggctacgctt tcggtcactt tgaacaccgc aggcaacgtg ccatccgcaa ataagcgctt
     2341 cccactgccc aaagtgatcg gatacagcat cagccagaac acatcaacca agtcatgctt
     2401 cataagcgtc tgaagaagtt caccacttcc ccacacatgc aagtctggtc ctggctgctg
     2461 tttgagcttg gcaacttcgc cagcgatgtc tccgcttaag aacacagaag gctgccattc
     2521 actggatgtc ctagaatttg acgcaacata cttggtcgcc ttgttgacat tcggccatat
     2581 atcgccatga ttgggccaat aaggctccca tatctcaaac gttttacgcc ccaacaacaa
     2641 gtcaaacgga ctgttcatct gctttcgcag aagcttgcca ctgatgtcat caccatagtt
     2701 ggcgatccac ccgccttgcg taaaaccgcc gctggtatct tcctctggtc cgcccggcgc
     2761 ctggatcacg ccatccagag agatgtgctc aagtacaaca atttttctca tggtcactcg
     2821 ccgtcgtacg cttttttcaa atctgcgatg atgagttttt tcatttgaag catggcttcc
     2881 atgccgcgct cggctttgac tgagtcaggt ccgccaatgt aatcgttcaa ctgcttcgga
     2941 acgatctgcc acgacaagcc ccatttatcc ttacaccagc cacaagattc cgcttcagga
     3001 attgctgaca acttgtccca gcaataatcc acttcggctt gatcttcgca gaggacatgc
     3061 atcgaaaatg cttcggtgaa tttaaacatg ggtcccccgt tcagcgccat gtactctacc
     3121 ccatccaact caaacgagac agtccacgca ttgccgtcgg gtttgcggtg aacacttagc
     3181 accttagagt tcttaaaaat cgagacataa taattcgccg cctcttctgc ttggggatcg
     3241 tcgtaccata aaaacggggt gatcttgctc atttcaatct cctttggctt tcttcttatt
     3301 tactgcgaga tgcagtttga cgactctttc aatcaacttg atcggaagcg gctcatcgag
     3361 tgcaaatttg accgtgcctt tgccatccac atactttgcg atctcacgtt cgaacgcctc
     3421 gctccccgcc ggaaccgggt acagcgacac atgttttttc caccccgcaa aatggatgaa
     3481 attcttgcca tttcgttcaa acgccgccat ttgataactg attctttctt ttgcatccgg
     3541 caccagagaa cgaatcaact ttcggatctg ttgcatatag tcctgcgact ctggcggaca
     3601 tgccacaata tattcatcaa tcgattgaaa attagttttc gccattttgt ccttccatga
     3661 gtgccaacaa tttcgtactt gtattccaat tacggatagt catattctga tagaccggca
     3721 tgccaataat ctttgtgaga tagctttgcg aagcgcggct gatgagacgt gaaaaataaa
     3781 gcacctgttt acctgcgtga gccatatcca ctccctcgcg gactttcaca ctttccatcg
     3841 cttccttggg agttagcggc tctttcaaga aaatgacatc gtaccgaaac ttcttttcat
     3901 cttcgccaaa tccatccggc gcagacttaa taatggcagc cagttccttt tgagagatta
     3961 ctacaacctt cgccacaaaa ttaaaacgct tggaaagtcc cttttcaatt ttggtcgtca
     4021 acgccgcttt atccttatct tcagattgaa ccaggacatt tccgctttga atgtaggtga
     4081 ccacattcga aaagcccatc tcttcaaaac tggctttcaa atccgccatt ttgatgatgt
     4141 tcttgccgcc aacattgatt ccgcgcaata ggacaaggta
//
LOCUS       DAOQNJ010000191         4168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13602_length_4168_cov_7.606613, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000191 DAOQNJ010000000
VERSION     DAOQNJ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13602_length_4168_cov_7.606613"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLG52_06775"
     CDS             complement(<1..232)
                     /locus_tag="PLG52_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB41185.1"
                     /translation="MSEPLLKVESLSKNFDRLPVLRDVSFQVDKGEVIGLVGRQGAGK
                     STLLHLLGGAIPASSGTINYLGKSRRFFNRLEA"
     gene            298..1347
                     /locus_tag="PLG52_06780"
     CDS             298..1347
                     /locus_tag="PLG52_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB41186.1"
                     /translation="MSLVRVLLADDHTVVRAGLRNALEVLPNLEIVGEVGDGIELMNA
                     ISQSPIDLLVMDASMPDFEPISAVQQIKEKYPSIKILVVSAYNDESYVVGLLKAGASG
                     YHLKDQPLSDLQLAAQRVLSGQRWISSNLIDRLLTRQAPTPSTNLPYLTRRQRELLRL
                     ISQGADNRNIAQALDLSIKTVENHLTALYRVLGVDSRLKALNYILRHPELLASTGQEM
                     ADSNPSVKGQEFTFLIVDDNPRYRQQLGRLIGKIYPSIKLYEADTTAEALSISKQVQP
                     QLAFIDVVLDDEDGIQCARKLRTVSPQTRIVVISAYPDKEFRRQALSAGVDVFLDKKD
                     LDTASVQQVLEDALR"
     gene            complement(1352..2581)
                     /locus_tag="PLG52_06785"
     CDS             complement(1352..2581)
                     /locus_tag="PLG52_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNB41187.1"
                     /translation="MKITADQAFLRETNLSLVVRLIHNQSPLSRAQLAGITGLNKSTV
                     SSLVDELIERGLVYETGSNTGGTGRPATLLEINPNAGHIIGVELGVDFVSVAVTDILG
                     NIVWRRREDANPADDQEKMINQTLQIVKDAMSIGKRKNYKFLGLGLSTPGTVDIQKGL
                     LIFAPNLHWHNVPFVQIFSEQTKLRVFVENDANAAAIGEHLFGTARHTNDFLFVFAGV
                     GIGGGLFLDGKLYRGKNGYAGEIGHSPIMAEPSQTVCHCGNRGCWETYANQYSIIQRV
                     QARLEVKRSSIIPKLMAEQNAPLGIPLIKQAADAGDREAIDSFYEAGHAMGQGLAGLI
                     NIFNPEKIVIGGPLSIAGEYMLPAIKQTVSQHAMPGIKPQAEIGLSPFGPDASLIGAV
                     AIVVDSILSSVPHIQKA"
     gene            complement(2783..4081)
                     /gene="eno"
                     /locus_tag="PLG52_06790"
     CDS             complement(2783..4081)
                     /gene="eno"
                     /locus_tag="PLG52_06790"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNB41188.1"
                     /translation="MDTTIESISALEVLDSRGNPTVEVEVLLMDGSWGRAAVPSGAST
                     GEHEALEMRDGDKKRYLGKGVANAVSNVNGVIAESLFGWDASDQRGIDAEMIAMDGTA
                     NKSKLGANAILGVSLAVAKAAANSFGMPLYRYLGGVYAHVLPVPMMNIMNGGAHTSWQ
                     STDMQEFMVMPFGAPTFSEGLRWGAEIYHALKSVLKEKGYSALVGDEGGYAPALKANN
                     EAIELILAAIEKAGFKAGRGEQVAIALDPAASELYDEKTKKYNLRKEGKELTGEQMVE
                     FWAKWVKDYPIVSIEDGLAQDDWDAWKSLVKTIGDKCQIVGDDLLVTNPERVRRAIKE
                     NAANALLVKVNQIGSLTETIEAVDLCQRAGWRAISSHRSGETEDATIADLAVALNAGQ
                     IKTGAPARSDRVAKYNQLLRIESELGDTAKYAGWDALKVK"
BASE COUNT         1025 a         1036 c          965 g         1142 t
ORIGIN      
        1 gcgcttccag ccgattgaag aaccgcctac tcttgccaag gtagttaatc gtcccagatg
       61 aggcgggaat ggctccacca agtaaatgga gtaaggtcga cttccccgct ccttgacgtc
      121 ccaccagccc gatgacttca cctttatcca cttgaaacga aacatcccgc aacacgggca
      181 aacggtcgaa gtttttggaa agactttcta ctttcagcag gggctcagac ataatttatt
      241 ttaacctgat tttgtgggct tcttttcagt ttttgttact tacggcataa tagatgcatg
      301 tctctcgtac gagttctttt agctgatgat cataccgttg ttcgggctgg tttgcgaaat
      361 gccctggaag ttcttcctaa tcttgaaatt gtgggtgaag ttggcgatgg cattgaattg
      421 atgaatgcca tttcacagag ccctatcgat ttattggtaa tggatgccag catgccagat
      481 tttgaaccga tctctgcggt tcagcagatc aaggaaaaat atccgtcgat caaaattctt
      541 gtggttagtg cttataacga tgaatcttat gtagtggggc ttttgaaagc aggtgcaagt
      601 ggttatcact taaaggatca gccattatca gatttgcagt tggcagcgca acgcgtatta
      661 agtggacagc gttggatatc gagcaatttg attgaccgtt tgttgacgcg tcaggcgccg
      721 acgccttcta ccaatttacc gtatctcacc cgccgccaac gtgaattgtt gcggctgatt
      781 tctcaaggtg ccgataatcg caatattgcc caagctttag acttaagcat caagacggtt
      841 gaaaatcatc ttacggcttt gtatcgcgtc ttaggggtgg atagccgctt aaaggcattg
      901 aactatatct tgcgccatcc tgagttgttg gcttctacag gtcaagaaat ggcagattcg
      961 aatccttccg taaaaggaca agaatttaca tttctaatcg tggatgataa ccctcgttat
     1021 cgtcaacagc ttggtcgttt gattgggaag atttacccat cgatcaaatt gtatgaagcc
     1081 gataccactg ccgaagcctt gtctattagc aaacaagtcc aaccgcaatt ggcatttatt
     1141 gatgtcgttt tagatgatga agatggaatt caatgtgccc ggaagttgag aaccgtttcg
     1201 cctcaaactc gcattgtggt gatcagcgcc tacccagaca aagagtttcg ccgacaagct
     1261 ttatctgccg gagtggatgt attcctcgat aaaaaagacc ttgatactgc ttcagttcaa
     1321 caagttcttg aagacgcctt gcggtagcct tttatgcttt ttgaatatgt ggaacgcttg
     1381 aaagaatact gtccacaaca attgcgacgg caccgatcaa gctggcatct ggaccgaatg
     1441 gagagagtcc gatctctgct tggggcttga tgcccggcat ggcatgttga ctcaccgtct
     1501 gtttgatggc tggcagcata tattcccctg caatgcttag cggacctcca atgacgatct
     1561 tctcggggtt gaaaatatta atcagcccag ccaatccctg ccccatggca tgaccagcct
     1621 catagaacga gtcgatagct tctcggtcgc ctgcatccgc tgcttgtttg atgagcggaa
     1681 tacctaatgg cgcattctgc tctgccatca atttggggat gatgctggag cgtttaactt
     1741 ccaaacgggc ttgcacccgc tgaataatgg aatattgatt ggcataggtt tcccaacaac
     1801 cacggttgcc gcaatgacaa acagtttgtg aaggctctgc catgataggg gagtgaccaa
     1861 tctcacccgc atacccattt ttgccgcgat acagtttccc atctaagaat agcccgccgc
     1921 caattccaac ccctgcaaac acaaaaagaa agtcattggt atgacgtgct gtgccaaaaa
     1981 gatgttcgcc aatggcggct gcattggcgt cgttctcaac aaaaaccctt agcttagttt
     2041 gctcagaaaa aatttgcaca aacggcacat tgtgccaatg aagatttggc gcaaaaatca
     2101 aaagaccttt ttgaatatct accgtgcctg gtgtggaaag tcccagccct aaaaacttat
     2161 agtttttgcg tttgccaatg gacattgcat cttttacgat ctgcaatgtt tgattgatca
     2221 ttttttcctg gtcatctgct gggtttgcat cttcacgcct gcgccaaacg atattgccaa
     2281 gaatatctgt cacagcaaca gacacaaagt ccacgccgag ttctacacca atgatatgac
     2341 ccgcgtttgg gttgatctct aacaaggtag ctgggcgtcc cgtcccacct gtattgctgc
     2401 ccgtttcata cactaagccg cgttcaatta actcgtcgac caggcttgaa acggtcgatt
     2461 tgttcagccc ggtaatacca gcaagttgtg cgcgcgataa tggagattga ttatgaatta
     2521 accttacgac caatgaaaga ttggtttctc gtaaaaatgc ttgatcggca gtgattttca
     2581 taggctccct aggattagta tgttggggca accaacaaat tatagccctt tgaagaggat
     2641 tgtcaaatta aacatcgaca ggcgcaaggt cgcttctttc tggaccttgt gcctgtttac
     2701 ccctttctag ctttttccta gccttgagtt tatgcaacta taaaaaaact ccgttgagtt
     2761 tgtctcaacg gagtttttca gcttacttta ctttcagagc atcccagcct gcatacttgg
     2821 cagtgtcacc cagctctgat tcgatgcgca gaagttgatt gtacttcgcc acacggtcag
     2881 aacgggcagg ggcgccggtc ttgatttgcc cagcattgag tgcaactgcg aggtcggcaa
     2941 tggtcgcatc ttcggtctcg ccagagcggt gagatgaaat ggcacgccag ccagcacgct
     3001 gacagaggtc cacagcttca atggtctcag tgagagaacc gatctggttc acttttacca
     3061 agagtgcatt cgcagcgttt tccttaatag cgcggcggac acgttcaggg ttcgtgacca
     3121 acaaatcatc acccacgatt tggcacttat cgccaatggt tttgacgaga gatttccagg
     3181 catcccagtc atcttgagca agaccatctt cgatggaaac gatggggtaa tctttgaccc
     3241 acttcgccca gaactcaacc atctgttcgc cggttagttc tttgccttcc ttgcgaaggt
     3301 tgtatttctt ggtcttttca tcatagagtt cagaagcagc cgggtcgagc gcaatcgcca
     3361 cctgttcacc gcgacccgct ttgaatccag ccttttcaat cgcagcgagg atgagttcaa
     3421 tcgcttcgtt gttcgccttg agagcaggag cgtaaccgcc ttcatcacca acaagggcgc
     3481 tgtacccctt ttccttcaac acagacttga gggcgtggta aatttctgcc ccccagcgca
     3541 aaccttcaga gaaagtcggg gcaccaaacg gcatcaccat gaattcttgc atatcggtgc
     3601 tttgccagct tgtgtgtgca ccaccattca tgatgttcat cataggcacc ggcaggacgt
     3661 gcgcatacac gccaccgagg tagcgataaa gaggcattcc gaaggaattt gccgctgcct
     3721 tggcaaccgc caaactcaca ccgaggattg cattcgcgcc gagtttagac ttgtttgcag
     3781 tcccatccat ggcgatcatt tcagcatcga tgcctctctg gtcagatgca tcccacccga
     3841 acaaggattc agcgatcaca ccattgacgt ttgagacagc attggctacg cctttgccga
     3901 gatagcgttt cttatcgcca tcgcgcattt cgagcgcttc gtgctcaccg gttgaggcac
     3961 cggagggaac cgcagcgcgt ccccatgagc cgtccatcaa taaaacttcc acttctacag
     4021 tggggtttcc gcgtgaatca agaacttcta gcgcggagat gctttcaatt gttgtatcca
     4081 tttaatttgc tcctaaatga aggcgacgat ttttgcttga tggtgttaat atcccaagac
     4141 aggtcgcccc tgatttttaa ttttcgcc
//
LOCUS       DAOQNJ010000192         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13644_length_4161_cov_7.919630, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000192 DAOQNJ010000000
VERSION     DAOQNJ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13644_length_4161_cov_7.919630"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="PLG52_06795"
     CDS             complement(<1..1265)
                     /locus_tag="PLG52_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB41189.1"
                     /translation="MQSQTTSETSQQKATERVYRTWREGFIISLLVGVLIFGAVALLP
                     ALNASESIVIDGIFISTYIIVALATIFKFPYNIRVTAFLLSVYVLGIGELISLGILGD
                     GLFYFIALVIFATMLVSPRAGSVSIAISIITFIVIGWLMISGRFDPLFPVVAPAKLAD
                     WFSASAALIMFGAVIIIGFGQLEKEFAGAQAKITSTLDELREERNNLDNRVQERTNQL
                     RRINEVGRAVAAILDINEILPLAAKYIQNEFDFYYTAVYLLDITGQWADLKEAIGETG
                     KVMKERKHRVNTTGKSLVAQVIRSKTGQIMQDTNQIRQENPMFPYTRSLIVVPLVVGD
                     TILGALEMHSSKDNEFLPQDLDAYQNMANGISIAIENARLFQEAQQSIMEMRATQRQY
                     LETAWSSLASEKSLDYALGDSDLADTNSL"
     gene            complement(1265..>4161)
                     /locus_tag="PLG52_06800"
     CDS             complement(1265..>4161)
                     /locus_tag="PLG52_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB41190.1"
                     /translation="AMGVFLYYGTSRAIKPLQSLSEMARKFAEGDRSLRADSINADNE
                     IGQLSRSFNYLADQIEDHYTSLEKKVEERTLQIRTAAEVAQSVTTIPNLDEMLRKTAE
                     LLVQQFNFYQTSVFLVDRSGKNIDFKTGYGSGTKDLTERKYRVQVGSSSILGWVAANN
                     KVRIASDTIEDELHLQNEFLPGTRTEACVPISINDQVLGVLDVQSTQPSAFSADAILM
                     LQTLASQLATAIQTAGLVDSSQVNFQELDRLYRSSRLIEAAKDEAEILRVGGQILKPA
                     PYPIVFLQLNSDSIRIINVSDMLRNDLSAELTQNLQANIQELNSLLMRGSVIVSGTSV
                     ELSKPVKDILRAFGLLNAVAIPVKGQEALKAIVLVGSQGEIPTGSSVQPYENLADLMS
                     VALQRASAFVQTERHLQEVETLASMNELIASTSDIQDFFHALHGKIQQIIGDYSLIVA
                     IYDEPTNSISIPFSYEAGKIQAIPTFPLGEGLTSILIRTRQPLLLVKDTERRAAELGA
                     KIVGKPAKSWMGAPLIVQNTVTGALIIQDVEHEGSFNEDDLKFFTTIASQVAGVIKNV
                     QLLDESKQKALQVETAAEIARDISGSLNLDELLSKAVNLIRDRFNFYHAGIFLLDIPG
                     EFAIIREATGEAGAQMKRQGYKIGVGSKSIVGYVTGKGETLVVSDTTKDITYYPNPLL
                     PETRAEAAFPLRVGERILGALDVQSIQPYGFHEDNLRSLQILADQLAIAVANTELFAE
                     TQEHLSQHRLLHHITSSAASGTTLEEALESAVSGLQVTLGGDRVIILLVDREKRILEV
                     KASIGYSEDISSMTVEVGKGVTGWVAAHRRPLRIRDVREDSRYIETSPNTRSEMAIPL
                     IYRNEILGVINVESELVDAYSENDEEMLGTLGGSLAAVIANARLVEQIRAQSERERLI
                     NEITARIRRSTDIQSILHTTASEISRVTGARYTKIQVEPKSADTPSDERQ"
BASE COUNT         1085 a          961 c          953 g         1162 t
ORIGIN      
        1 tcaagcgaat ttgtatccgc taaatcagaa tcacccaagg catagtccaa agatttttca
       61 gaggcgagcg aactccatgc agtttcaagg tactgtcttt gagttgctct catttccata
      121 atactttgtt gagcttcttg gaaaaggcgg gcattttcaa tcgcgattga aattccattt
      181 gccatatttt gataagcatc aagatcttgt ggcaaaaatt cattgtcttt tgatgaatgc
      241 atctcaagag cgcctagtat cgtatcgcca actactaaag gcactacgat cagagatcgt
      301 gtatatggga acatcgggtt ttcttggcga atttgattgg tgtcttgcat gatttgtccc
      361 gttttcgaac ggatcacttg ggcaaccaaa cttttcccgg tagtgttgac gcgatgcttg
      421 cgttccttca tcaccttgcc ggtttcgcca atagcttctt tcaggtcagc ccattgcccg
      481 gttatatcca agagataaac agctgtgtaa tagaaatcaa attcgttttg gatatatttt
      541 gcggcaagcg gcaagatttc attgatatca agaatggctg caaccgcacg ccctacttca
      601 ttaatgcggc gaagctgatt tgtgcgttcc tgaactctgt tatcgagatt atttcgttct
      661 tcgcgtaatt catcgagtgt agaagtgatt tttgcttgcg cacctgcaaa ctctttttcc
      721 aactgcccga aaccaatgat gataactgct ccgaacataa tcagcgcagc gctggcgctg
      781 aaccagtcgg caagtttggc aggagccaca acagggaaga gcgggtcaaa acgcccgctg
      841 atcatgagcc aaccgatcac gataaaagta ataatactaa ttgcaatcga gacactgccg
      901 gctctaggcg ataccagcat tgtggcaaaa atgaccagcg caatgaaata gaaaagaccg
      961 tctcctaaga tcccgaggct gatgagttcc ccaatgccta acacatacac gctcagtaag
     1021 aatgcggtta cgcggatgtt gtaaggaaac ttgaaaatcg tagcaagagc cacgatgata
     1081 taagttgata taaagatgcc atcgataaca atgctttcag aagcattgag cgcaggtaag
     1141 agtgctacag cgccaaatat cagtacaccg accaaaagag aaataataaa accttcgcgc
     1201 caggttctat aaactctttc agttgctttt tgttgagagg tttctgaagt ggtttgagat
     1261 tgcattattg cctctcatca ctaggtgtgt cggcgctttt cggctccacc tgaatttttg
     1321 tataacgcgc acccgtaacc cttgatatct cactggcagt tgtgtgtaga atggattgaa
     1381 tatctgtgga tctgcggatg cgggcggtga tctcattgat aagacgttcg cgctcagact
     1441 gcgcccgaat ttgctccacc aaccgggcat tagcgatcac tgctgccaaa ctgccgccga
     1501 gcgttccaag catttcctca tcgttctcag aatatgcatc aactaattca ctttccacgt
     1561 tgatcacacc cagaatttca ttgcggtaaa ttaaagggat tgccatttca gagcgagtat
     1621 tgggactggt ctcaatatag cgtgagtctt cgcgtacatc gcgaatccgc aaggggcgtc
     1681 tgtgagccgc cacccagccg gtgacacctt tgccaacctc aaccgtcatg ctggaaatat
     1741 cttccgaata cccaatcgaa gctttcactt ccaatatacg tttttcgcga tcgaccaata
     1801 aaataatcac ccgatcaccg cccagtgtta cttgaagtcc actgacagcg ctttccagtg
     1861 cttcttctag tgtagtacct gaagcggcag aggatgtgat gtgatgcaat agacgatgct
     1921 gtgagagatg ttcttgggtt tctgcaaaca attctgtgtt tgcaaccgcg attgcaagtt
     1981 gatctgcaag aatctgtaaa cttcgcagat tatcttcatg aaaaccatag ggctgaatgc
     2041 tttgaacatc taatgcgccc aaaatacgtt cgccaactcg caaggggaaa gcggcttcgg
     2101 cgcgggtttc tggaagaagt gggttggggt agtaggtgat atcttttgta gtatcactga
     2161 ctaccagcgt ttcgcctttt ccagtgacgt accccacaat cgattttgag ccgacgccga
     2221 tcttatatcc ttggcgcttc atttgagcac ccgcttcgcc agttgcctcg cgaatgatgg
     2281 caaattcacc cggaatatct agcaagaaaa ttccggcatg atagaaatta aaccgatcgc
     2341 ggattaagtt gacagccttg ctcaaaagtt cgtcgaggtt caatgagcca ctaatgtcgc
     2401 gagcgatttc agctgcagtt tctacttgca acgctttttg cttgctctca tcgagaagct
     2461 gaacgttctt gatgactcca gctacctgac ttgcaatggt agtgaagaac ttcaggtcat
     2521 cttcattaaa cgagccttca tgttcaacat cttggatgat caatgccccc gtgaccgtgt
     2581 tctgcacgat caatggcgcg cccatccaag atttggctgg cttcccaacg atctttgctc
     2641 caagttctgc ggctcttcgt tctgtatcct taaccagtaa taggggttgg cgcgtacgaa
     2701 ttaatattga tgttaaccct tcaccaagtg gaaaagttgg aattgcctgg attttgccag
     2761 cttcataact aaatggaatg ctgatagagt ttgtcggttc atcgtagatc gcaacgatca
     2821 aactgtaatc gccaataatc tgctgaattt ttccatgcaa agcgtggaag aaatcctgaa
     2881 tatcagatgt agaggcgatc aattcattca ttgacgctag tgtttcaacc tcttgtaagt
     2941 gtctttctgt ttgtacaaag gcactagcac gttgaagggc aacagacatt aagtctgcga
     3001 ggttttcgta tggctgcacc gaagaaccgg tgggaatttc gccctgtgag ccaaccagta
     3061 cgattgcttt taaggcttcc tgtcccttta ctgggatggc aaccgcattg agaagcccaa
     3121 aggcgcgaag aatgtcttta accggcttgg acaattccac gcttgtgcca gatacgatga
     3181 cagaaccacg catcaacaat gaatttagtt cctgaatgtt ggcttgtaaa ttttgggtca
     3241 actcagcaga aaggtcgttt cggagcatgt ctgaaacgtt gataatcctg atactgtcac
     3301 tatttagttg aaggaagacg atagggtagg gggctggctt tagaatttgc ccgccgacac
     3361 gtaaaatctc tgcctcatct tttgccgctt cgatcaaacg gctggaacga tacaagcgat
     3421 ctaattcctg gaagttgact tggctggagt caaccaaccc tgccgtttga attgcagtgg
     3481 cgagctgact tgccaatgtc tgtagcatca gaatcgcatc ggcactgaaa gccgacggtt
     3541 gtgtactttg gacatctaat acgcccaata cctgatcgtt gatcgaaatg ggcacacagg
     3601 cttctgtgcg tgttccgggt aggaactcgt tttgaagatg tagttcgtct tcaattgtgt
     3661 ctgaggcgat gcgaactttg ttgttagcag caacccagcc caaaattgag gaagagccga
     3721 cttgtactcg atactttcgt tctgtaagat ccttcgtgcc agaaccgtat ccggttttga
     3781 aatcgatatt ttttccactg cgatcgacca gaaacacact tgtttgataa aaattgaatt
     3841 gctgtacaag cagttccgcg gtcttgcgca gcatttcatc aaggttggga atggttgtaa
     3901 cactttgcgc cacttcggcg gcagttcgaa tttgaagcgt tcgttcttcc actttctttt
     3961 caagagaggt gtaatggtct tcgatctggt ctgcaagata attaaatgac cgcgaaagtt
     4021 gaccgatctc attatctgca tttatagaat cagcacgcag gcttcgatca ccttctgcaa
     4081 atttgcgagc catttcagaa agggactgta aaggcttaat tgcgcgactt gttccatagt
     4141 acaagaaaac acccatcgca a
//
LOCUS       DAOQNJ010000193         4135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13772_length_4135_cov_8.618873, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000193 DAOQNJ010000000
VERSION     DAOQNJ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13772_length_4135_cov_8.618873"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLG52_06805"
     CDS             complement(<1..427)
                     /locus_tag="PLG52_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNB41191.1"
                     /translation="MRKRVVVTGLGCVSPVGNNVKDTWEALLAGKSGAAPITAFDASA
                     HKTKFASEVKGFDGAALFGTRESRKMDRFTQFATAATLEALEHSGLKIDESNRDRVGI
                     LIGTGIGGIITTIEQYENLKERGPERVSPFLIPMMIADSA"
     gene            complement(579..1772)
                     /locus_tag="PLG52_06810"
     CDS             complement(579..1772)
                     /locus_tag="PLG52_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB41192.1"
                     /translation="MKLTLLLDLDETLLNTNQDVFFPAYIQALSTNFASQLDPKLVMR
                     ALMTSIEKMNQNDDASRTLKDVFSDEFCPQVGMTRQELDAHIDVFYSQVFPTLRGITS
                     QIAETQPFIEWAMKQGYRLVVATDPLLPRPATRQRVQWAGLDPDQFELISTFEDFHFL
                     KNFSAYYAEVLGQLGWQEGPVLMVGNDAERDILSAKRLGLATFFVETESGSTSGPEAG
                     PRGTLSDLRRYLESTDLASLTPSFKTKEAIMAIQASVPAVLDTFFRGLSAETWKHKPA
                     SSEWTLTELVCHLRDTEREIHHMQLKLFNGQDEPFIPRPDTGIWANQRDYLHEDGPSA
                     LHEFAAARSMTLSMLQNTPDVEWDRKARHAIFGPTNFREVAGFIADHDRLHVQQALSL
                     LKKLQ"
     gene            complement(1803..2135)
                     /locus_tag="PLG52_06815"
     CDS             complement(1803..2135)
                     /locus_tag="PLG52_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41193.1"
                     /translation="MKTPAGHECPHFYGDYFRGRNVEECRLLKSQNQAWSPDLCKTCP
                     VPAIARANSCQFMKLNVVVARPLTAIFQRRVQVQAYCEKSNQNVSEPQVGCGECHALP
                     FKFEIKED"
     gene            complement(2141..2698)
                     /gene="efp"
                     /locus_tag="PLG52_06820"
     CDS             complement(2141..2698)
                     /gene="efp"
                     /locus_tag="PLG52_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNB41194.1"
                     /translation="MIDVNELRKGVTFELDGNLYKVLDYSHNKTGRGNATIRVKARNL
                     LTGANIERTFNSGQSVQDVSLDFANVSYLYSDGDLYYFMDKVTFEQPGIKAESLGESA
                     QYLKEGMEVKLTFYKGEAIDVEMPTSVDLKVVEAEMAIRGDTATGVTKKVTTETGLTV
                     QCPNFVNVGDTIRVDTRTGEYVTRV"
     gene            complement(2856..>4135)
                     /locus_tag="PLG52_06825"
     CDS             complement(2856..>4135)
                     /locus_tag="PLG52_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNB41195.1"
                     /translation="PPPPSLFANGSCVYGAGVAQFNITNNGGDMKSPYKYTITDSAGN
                     IVQSGTVQLKAGESLTLKVSGTFDSLLLTILDGAGTTVLTSMANCAETGGLAVAPAEP
                     QVCIQCLIFHTFRDDNLEVYRLDGVEGQPGFKLYNLSQDEAVDSRPSRAPNDSWVVFQ
                     SNRDGNVELYYTDLLGSGEAIRLTDTEANNTNPMYGPDAETIVFQSDRNGRSDLFTID
                     QNTGEEVQLTSSSADDVNPFFSPDINKLVFQSNRNGNWDLFVLDLETGTDTQLTNSTE
                     DETFPAWSSNGKQIAYIKEVNGATDLYVIDLETGTERQLTNDGKTTNAVWSPEGDRIA
                     YQSERSGNLDIYSYDFRDNKEYRVTDFDGKDSGPTWDCGGTNLAFTSVRDGDLNIFQV
                     FWQGGSAGNMTIDPASDKWSQWRPSNDISSVGY"
BASE COUNT         1074 a          999 c          955 g         1107 t
ORIGIN      
        1 ctgcgctatc tgcaatcatc attgggatca aaaatgggct tacacgttct ggtccacgtt
       61 ctttgagatt ctcgtattgc tcaatggtgg taataatgcc gccaatgccg gtgccgatta
      121 aaatacccac tcggtcgcgg ttcgattcgt cgatcttcaa gcctgaatgt tcaagtgctt
      181 ccaacgttgc cgcagtagca aactgggtga agcggtccat tttgcgagat tcacgggtgc
      241 caaataaggc agctccgtca aagcctttta cttctgaggc aaacttcgtc ttatgagcgc
      301 tggcgtcaaa tgcagtaata ggggctgcac cagacttgcc cgcaagcaaa gcttcccaag
      361 tatcttttac attatttcca accgggctaa cacaccccaa tcccgttaca acaactcgtt
      421 ttctcatgta ccagtactcc ttatcgttaa tttgcaaaaa atagaacaag caaacttgtc
      481 ctatttaaca caagattcgg gaattggaca cgcgggaata tggcacctag gcttaaaaaa
      541 ccaggtcatc agacaatatt aggaggcatt cccaatcgtt attgaagttt tttcagcaaa
      601 gaaagagctt gttgaacatg taatctatcg tgatctgcaa taaaaccagc cacttcacgg
      661 aagttggttg gtccaaaaat tgcatggcgc gctttgcgat cccactctac atctggcgta
      721 ttttgtaaca tacttaatgt catagaccgt gctgcagcaa attcgtgcaa tgcagaagga
      781 ccatcttcat gtaaataatc tcgttggttt gcccaaatcc ctgtatctgg gcgtggaatg
      841 aacggctcat cttgtccgtt gaaaagcttc aattgcatgt gatgaatctc gcgctctgta
      901 tcacgcaaat ggcagaccaa ttctgtcaac gtccattcgc tggaagcagg tttatgtttc
      961 catgtctccg cacttaatcc gcggaagaag gtgtctaaca cggcaggcac agacgcttga
     1021 atagccatga tagcttcttt tgttttaaag gatggagtta aggatgcgag atcagtagac
     1081 tcaaggtaac ggcgtaggtc agataatgtg ccgcgcggtc ccgcctcagg tccagacgtt
     1141 gagccggatt ctgtttccac gaaaaatgta gccagcccaa gcctcttcgc tgaaagaatg
     1201 tcccgctctg catcattccc caccatgaga actggaccct cttgccagcc caattgcccg
     1261 agcacctcag cgtagtaggc agaaaagttt ttcagaaagt gaaaatcttc aaaagtagaa
     1321 atcaattcaa attggtcagg gtctagccca gcccattgca cacgttgacg agtcgctggg
     1381 cgtggcaata acgggtcagt cgccacaacc aaacgatatc cttgcttcat tgcccattca
     1441 ataaatggct gcgtctctgc aatttgtgaa gtgataccgc gcaaagttgg gaacacttga
     1501 ctataaaaca catcaatgtg tgcatctagc tcttggcgcg tcatccccac ttgcggacag
     1561 aattcatcac tgaacacatc tttcaatgta cgggaagcat cgtcattttg attcatcttt
     1621 tcgatgctcg tcattaacgc acgcatgacc agttttggat ctaactgcga tgcaaaattg
     1681 gtcgacaatg cttgaatgta cgcagggaaa aagacatcct gattggtgtt cagcagggtt
     1741 tcgtcaaggt caagcagtaa tgtgagcttc ataatgaaaa agactccgta ggagtctttg
     1801 ggttaatctt cttttatttc gaatttaaaa ggcagggcat ggcattcgcc acaccccacc
     1861 tgaggttcag aaacattctg atttgatttt tcgcaatagg cttgcacttg cactctacgc
     1921 tgaaatatag ccgttagtgg acgagccacc accacattca atttcatgaa ttgacatgaa
     1981 ttcgcgcgtg caatagcggg cacagggcaa gtcttacaaa ggtctggaga ccaggcttgg
     2041 ttttgcgatt taagcaagcg acattcttcc acgtttctgc cgcggaagta gtcgccataa
     2101 aaatgcgggc actcgtgccc agcaggtgtt ttcattccta ttagacgcgg gtaacgtact
     2161 cgcccgtacg agtgtctaca cgaatggtgt cgccaacgtt gacgaagttc gggcattgca
     2221 cagtgagccc ggtttcagtg gtgaccttct tggttacgcc ggtggcagta tcgccgcgga
     2281 tcgccatttc agcttccacg actttgaggt ctacagaagt gggcatttcc acatcaatag
     2341 cttcgccttt gtagaaggtc aacttaactt ccatgccttc tttgaggtac tgggcgcttt
     2401 caccaagtga ctcggcttta atgccaggct gttcgaaagt gaccttatcc ataaaatagt
     2461 aaaggtcgcc gtcactgtag aggtaagaaa cattagcaaa atcgaggctt acatcctgca
     2521 cggattgacc cgagttgaag gtgcgttcaa tgttggcacc ggtaaggaga ttacgcgcct
     2581 taacacggat ggtcgcattg ccgcgcccgg ttttgttgtg tgaatagtcc aacaccttat
     2641 agaggttgcc gtccaattca aaagtgacac ctttgcgaag ttcatttaca tcgatcattc
     2701 ttgcattttc cttggttatt tggtttttgt ataagcgggc ggattatacc aacgagtagc
     2761 gaatcatggc taggatggga tcattaggaa agaatgaggc acaaaaaaga gcctctcctc
     2821 aaaaaacagg agaggctctt tgcaatcaaa tcttattagt agcccacact tgagatgtca
     2881 ttgctaggtc gccactgtga ccacttatca gaagcggggt cgatggtcat atttccagcg
     2941 gaaccaccct gccagaagac ctggaagatg ttcaagtctc cgtcacgaac cgatgtgaag
     3001 gccaggtttg tgccaccaca gtcccatgtc ggacccgagt ctttgccatc aaagtctgtc
     3061 acacgatact ccttgttatc tctaaagtca tagctataga tatccaagtt tccactgcgt
     3121 tcagattgat aggcaatgcg gtcaccttca ggggaccaaa cagcattggt cgtcttgcca
     3181 tcgttagtga gttgtctctc tgttccggtt tcgaggtcaa tcacatacag gtctgttgca
     3241 ccgttgactt ctttaatgta tgcaatctgc tttccgtttg aagaccaagc tgggaaggtt
     3301 tcatcctctg tcgaatttgt cagctgagtg tccgtaccag tttccaaatc gaggacaaaa
     3361 agatcccaat tgccattacg gttgctctgg aagaccagct tgttgatatc tggcgagaag
     3421 aacgggttga catcatctgc tgaggaactg gttaactgga cttcttcacc tgtattctgg
     3481 tcaatcgtga agagatcgct acgaccgttt ctatcacttt ggaagacgat tgtctcagca
     3541 tctggaccat acatcgggtt ggtgttattc gcttctgtat ccgtcaaacg aatcgcttca
     3601 ccactaccaa gtaagtctgt gtaatacaac tcgacatttc cgtcacggtt actttggaac
     3661 acaacccaac tatcattcgg tgcgcggctc gggcgactgt caacagcttc gtcttgcgac
     3721 agattataga gcttgaagcc gggctgtcct tccacgccat ccaatcgata cacttcaaga
     3781 ttatcatcgc ggaaagtatg gaagatcaaa cactggatac aaacctgcgg ctctgcagga
     3841 gcgacggcta atccgccagt ttcagcacag ttcgccatgc tagtgagtac tgttgttcct
     3901 gcaccgtcaa ggatggtcaa aaggagcgag tcgaaggttc cagaaacctt aagggttaaa
     3961 ctctcgcctg ccttcaactg aaccgtgcca gactgaacga tatttccggc actatctgtg
     4021 atcgtgtatt tgtatggcga tttcatatcg ccgccattgt ttgtgatatt gaattgagca
     4081 acaccagcgc catacacaca agatccattc gcaaacaaac taggaggcgg aggtg
//
LOCUS       DAOQNJ010000194         4107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13913_length_4107_cov_9.105133, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000194 DAOQNJ010000000
VERSION     DAOQNJ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13913_length_4107_cov_9.105133"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..857
                     /locus_tag="PLG52_06830"
     CDS             222..857
                     /locus_tag="PLG52_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNB41196.1"
                     /translation="MNFFGKRVLFIGAHPDDIELGCGALLHHIVKNTDASVLCVTLSD
                     NQKNPDLKKVKNEHLNAMQVLGVPSDNVVLGPFTTRVFPDSRQEILEYFLKLRKEFKP
                     DLIFTHSKQDVHQDHNTMTDEALRAFRGITVLGFDVVRSSYGFFPHFLVEVTEEDVNK
                     KIEALSQYDTYQDRYYFNAELTRSIMVRHGALAERPFAEGFDILRIVGKFE"
     gene            complement(892..1773)
                     /locus_tag="PLG52_06835"
     CDS             complement(892..1773)
                     /locus_tag="PLG52_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB41197.1"
                     /translation="MSSLSNIAIKLIPASHFSIDELTGIYNQTRVDYMVPMPMNSARL
                     MEYIEFYDVDLDRSVVAIEDGELRGVAMLGVRAERTWVTRLGVIPSTRRGGVGESLMQ
                     SLLEQSARLGIDFTMLEVIKNNVPAHRLFLKLGFYEIGELLILRRAPSKKLVEPVIAD
                     ARRLERYEALDLVGYDRGTQPWTNQSESMSHAQDISGLYLTLQDGSQGWLVYQRQKFT
                     LTHFIYKTEVGDPATMAYAFLSHLHHQYPRLDTHIENIQVNDPHLPGFQKMGYVESFR
                     RIEMWRGNPPSSIMPSK"
     gene            complement(1783..2154)
                     /locus_tag="PLG52_06840"
     CDS             complement(1783..2154)
                     /locus_tag="PLG52_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB41198.1"
                     /translation="MTRILIVEDVPDNVELARKILTSRGYEVIHAINAEMGYNLAIQE
                     RPDLVLLDLGLPDYDGLTLAGQIRENPTFERLPIIAFTAWPQETAKNMAESYGCNGFI
                     SKPIIRVNEFIAQVESFLLNG"
     gene            2332..3465
                     /gene="sucC"
                     /locus_tag="PLG52_06845"
     CDS             2332..3465
                     /gene="sucC"
                     /locus_tag="PLG52_06845"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNB41199.1"
                     /translation="MKLHEYQSKNIFSKFGIPIPKGRVATTAQEAKQIAEELGGRVVI
                     KSQVLVGGRGKAGGVKLAKDPAEAEQLATQILGMEIKGLPVRKVLVDEASAIDQEIYF
                     AITDDRAAKKPVLIASAAGGVDIEEVAAKTPEKIIKVNIDPLLGLREYQARDVAVAID
                     LPRDYWKDFTKIAMGLWHVYQSTDASLAEINPLVITKDKKMIALDGKMMIDDNALFRQ
                     SDLAEIRDTDEDAPAEIEARKYGLSFIKLDGNIGCMVNGAGLAMTSMDVIKLFGGDPA
                     NFLDVGGGAGAEKVAAAMRIILTDPNVKAVLFNIFGGITRCDEVAKGILVAMDEVKPK
                     VPMVVRLVGTNAEEGRRLLENANMITAETLADAAKKAVAAAKG"
     gene            3471..>4107
                     /locus_tag="PLG52_06850"
     CDS             3471..>4107
                     /locus_tag="PLG52_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNB41200.1"
                     /translation="MSILVDKNTRLLVQGITGNEGLFHTTQMAAYGTNIVGGITPGKG
                     GEWVLDGKIPVFDSVKSAKDATDANATIIFVPARFAPDAMFEAADAGIPLVICITEGV
                     VIHDMMRVRNYLDQKKVRLIGPNCPGLLTPGEAKVGIIPGNIAIPGNVGVVSRSGTLT
                     YEVLYAMKNLGMGASTCVGIGGDPVNGTNFTDVLEMFEHDPKTEKVIMIGEI"
BASE COUNT         1113 a         1020 c          959 g         1015 t
ORIGIN      
        1 attacgtccg cgcttcattg agtgaagatc gcagactgtg gacttccatg ttcaatggca
       61 aggatgaata cgaagccttg atcggtaaga tcacagctgt ggctcaaaag tggctcgacc
      121 gtgacggcaa attggtaatg accaaagtcg ttggcggcat tctggcgaca aaataaacgt
      181 tatgtagggg cgactcgcga gtcgccccta cgggtttcaa tatgaacttc ttcggcaaac
      241 gagtcctctt tattggcgcg cacccagacg acatcgaatt gggatgtggg gcgcttttgc
      301 atcatatcgt taagaataca gatgcaagtg ttttatgcgt caccctttct gataatcaaa
      361 aaaaccctga cctaaaaaag gtcaagaatg aacatcttaa cgcaatgcaa gtactgggtg
      421 tgccttcaga taatgtagta ttaggaccct tcaccacacg cgtctttccc gattcgcggc
      481 aggaaattct tgaatatttt ctaaaactgc gcaaagaatt caagcctgat ctaattttta
      541 cccattccaa acaagatgta caccaagatc acaacacaat gaccgatgaa gccttgcgcg
      601 cctttcgagg tattactgta ttgggttttg atgtggttcg ttcatcttat ggtttcttcc
      661 cgcactttct cgtggaagtc actgaagaag atgtaaacaa aaaaatcgaa gccttatccc
      721 aatacgatac ctaccaagac cgctactact tcaacgccga gctcacccgc tctatcatgg
      781 tccgccatgg ggcgttggca gagcgtccgt ttgcggaggg gtttgatatt ttgaggattg
      841 tagggaagtt cgaataatat aaaaaataaa acaggctttc agaagcctgt tttattttga
      901 aggcataatc gatgacggag gattaccccg ccacatttcg atgcggcgaa aggattcaac
      961 atatcccatt ttctgaaagc ccggtaaatg tgggtcgttg acttgaatat tttcaatatg
     1021 cgtatccaac cgtggatact gatggtgcag atgggataag aaggcatatg ccattgtagc
     1081 tgggtcaccc acttcggtct tgtaaataaa atgcgtcagc gtaaatttct gccgctgata
     1141 cacgagccag ccctggctcc cgtcttgaag cgtgaggtag agaccggaaa tatcttgtgc
     1201 atggctcatc gattcggatt gattcgtcca tggttgagtc ccacggtcat atcccacaag
     1261 atccagtgct tcatatcgtt ccaatcgacg tgcatctgct attacaggtt ccactagttt
     1321 tttagatggc gcacgcctaa gaatcagcaa ctccccaatt tcataaaacc caagtttcag
     1381 gaacaatcga tgggcaggaa cgttattttt aataacttcc agcatggtga agtctatgcc
     1441 cagtctggca gattgctcca gcaagctttg catcaaggat tcaccaacgc caccccgccg
     1501 cgtggacggg ataacaccta aacgggtgac ccatgtccgc tcggcacgaa cccccaacat
     1561 agctacaccc cgtaattctc cgtcttcaat tgcaacaacc gaacgatcta aatctacatc
     1621 ataaaactca atatattcca taagacgcgc agagttcatg ggcatgggca ccatataatc
     1681 tacgcgagtt tggttgtaga tgccagtcaa ttcgtcaatg gaaaaatgac tagcggggat
     1741 taacttaatg gcaatatttg ataaagaaga cattcttaag ggttagccgt ttaagaggaa
     1801 actctctact tgagcaataa actcattcac acgaataata ggtttactaa taaaaccatt
     1861 gcagccatag ctttctgcca tgttttttgc tgtctcttga ggccatgcag taaaggcaat
     1921 gattggcagt cgttcaaagg ttgggttttc acgaatttgc ccagctaggg tcaaaccgtc
     1981 ataatcgggc aaccctaaat ccagcaacac aaggtctggg cgttcttgta tggcaaggtt
     2041 gtatcccatt tcagcattta tggcatgaat aacttcatac cctctcgaag taaggatctt
     2101 tcgagctaat tccacattat cgggcacatc ttctacaatt aggattcgcg tcatagttac
     2161 ctttggtaaa acttaaggtc aaagcaatgt taccacttgc agaaaaggat tccattgcaa
     2221 tttatcgcta gaagttcttg gcagaaatat agtattttac aacccgtgtc tatttcgaag
     2281 tggtataatg ccgatgcttt ttagcaaaaa tatactctcc gagggagaag aatgaaactc
     2341 cacgaatacc aatccaaaaa tattttttcg aaatttggca tcccaatccc caagggacgc
     2401 gttgcgacta ccgctcaaga ggcgaagcag atcgccgaag agcttggtgg tcgcgttgtc
     2461 attaaatcgc aggtgttggt aggcggacgc ggcaaagctg gcggtgtaaa acttgccaag
     2521 gaccccgccg aagccgaaca gctcgccact cagattctgg gcatggagat caaaggtctc
     2581 cccgtgcgca aagtcttggt ggatgaagcc tctgccatcg accaggaaat ctacttcgcg
     2641 atcaccgatg accgcgccgc taagaaaccg gttcttattg cctccgctgc cggtggtgtg
     2701 gacattgaag aagttgccgc caagaccccc gaaaaaatca ttaaagtaaa cattgacccc
     2761 ttgcttggct tacgcgaata tcaggctcgt gacgttgccg ttgccattga ccttccacgt
     2821 gattattgga aagatttcac caagatcgcc atggggcttt ggcatgtcta tcaaagcacc
     2881 gacgcctccc tcgcagaaat caacccgcta gtcatcacca aagacaaaaa gatgatcgct
     2941 ctcgacggca aaatgatgat cgatgacaat gcgctcttcc gtcaatcaga tttggctgag
     3001 attcgcgata ccgatgaaga tgcacccgct gaaattgaag ctcgcaagta tggtctgtct
     3061 ttcattaagt tagatggcaa tatcggctgt atggtcaatg gcgcaggtct ggcaatgacc
     3121 agcatggacg taatcaaact ctttggtggc gacccagcaa acttccttga tgttggaggc
     3181 ggtgctggcg cagagaaagt cgctgctgcc atgcgcatca tcctgactga cccgaacgtc
     3241 aaagcagtgc ttttcaatat cttcggcggc atcacccgct gtgacgaagt tgccaagggc
     3301 attctcgttg ccatggacga agtcaagccg aaggtgccga tggtcgttcg ccttgttgga
     3361 acgaatgcag aagaaggtcg ccgtttgctt gaaaatgcaa acatgatcac tgccgaaact
     3421 ctcgccgacg ccgccaagaa agccgtcgct gcggcaaagg gataatacaa atgagcattt
     3481 tagtagataa aaatacccgc cttcttgtgc aaggcatcac tggcaacgaa ggcttattcc
     3541 acaccacaca gatggctgcc tacggcacaa acatcgtcgg tggaatcacc cccggcaagg
     3601 gcggtgaatg ggtcttggat ggaaagatcc ccgtctttga ttcggtcaaa tctgccaaag
     3661 acgcaaccga tgcaaacgca accatcatct tcgtccctgc ccgcttcgca ccagacgcca
     3721 tgttcgaagc cgctgatgca ggcattcccc tcgtcatctg cattaccgaa ggtgtcgtca
     3781 tacacgacat gatgcgtgtg cgcaactatc tcgaccaaaa gaaggtccgc cttattggtc
     3841 cgaactgccc cggtctgttg acgcccggtg aagccaaggt cggcatcatc cccggcaata
     3901 tcgccatccc tggcaatgtt ggcgtggtct cccgctctgg cacattgacc tatgaagtgc
     3961 tctatgccat gaagaacctc ggcatgggcg catcaacatg cgtcggcatc ggtggtgacc
     4021 cggtcaacgg caccaacttc accgatgtgc ttgaaatgtt cgaacacgac cccaagaccg
     4081 aaaaggtcat catgatcggt gagatcg
//
LOCUS       DAOQNJ010000195         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13945_length_4101_cov_11.220465, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000195 DAOQNJ010000000
VERSION     DAOQNJ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13945_length_4101_cov_11.220465"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLG52_06855"
     CDS             complement(<1..402)
                     /locus_tag="PLG52_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41201.1"
                     /translation="MFACIWSAGETYLLFYVAARYRPTSKQLGRAWTIIATSALFSFL
                     GNLFWFGMSHFLHIYPYPSVADIFYLLSYPLMLIGILSFPYTSKTTLEKLKIYLETAT
                     VVISIGMALWFFVLRPVIDQTGTSDKTYLFFT"
     gene            complement(693..1037)
                     /locus_tag="PLG52_06860"
     CDS             complement(693..1037)
                     /locus_tag="PLG52_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB41202.1"
                     /translation="MELNVSDLDKGITLIKLDGRLDITGVNQIETKFAAYCSGENARV
                     IVDMAKVDYLASIGIRLLVTNAKSLKSRNGKIIILRPTQAVMEVLEMTNIPAIIPVYT
                     NLESAEAVLLGY"
     gene            complement(1115..3202)
                     /gene="ppk1"
                     /locus_tag="PLG52_06865"
     CDS             complement(1115..3202)
                     /gene="ppk1"
                     /locus_tag="PLG52_06865"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNB41203.1"
                     /translation="MTEIDLSHPSLYINREASLLEFQRRVLEEARDEENPLLERVKFL
                     AILGSNMDEFFMVRVSGIRKQVEANIMKLSEDGLTPREELAGIRKTAQDLMQDAQQTF
                     HRKLLPKLEKEGIHVLDYSKLSKSQKERADQYFKDVVYPVLTPLALDPGHPFPHISNL
                     SLNLAIVIRDKKGNEKFARLKVPDTLPRLIPIKKSSGSARKDGTIPFHHYFVWLDQVI
                     AANLDGLFPGLEVVSAHPFRIVRDADIEIQELEADDLLETMQQNIRKRKFGSVVQVAV
                     HPSMPEEMRDLLVDNLEITRNDIYSLPHPLGLASLWQLYNSVERHDLKYPPYIQRTPK
                     PLRNLDTSSEIFDAIRNENVLLHHPYESFIPVVDFLYAAARDPHVLAIKQTVYRVGSN
                     APVVEALLEAAERGKQVAVLVELKARFDEESNIGWARALEQAGVHVVYGLVGLKTHCK
                     VAMVVRKEGDGIRRYLHLATGNYNAVTSRIYEDLGMFTCDEDMGADATDLFNYLTGYS
                     TKKSYKKLLVAPVNLREQMEKLIRREIEHAKAGNKARIIFKMNSLVDMDMIQLLYQAS
                     QAGVKVDLLIRGMCCLRPGIKGVSENIRVLSVVGRYLEHSRIYYFQNNGNDEIYMGSA
                     DLMPRNLNHRVEVVFPVENKGQVKFLRDKILEAYLKDNTRARILKTNGTYERSKPDSN
                     GKRFDVQSHLMGE"
     gene            complement(3253..3753)
                     /gene="sixA"
                     /locus_tag="PLG52_06870"
     CDS             complement(3253..3753)
                     /gene="sixA"
                     /locus_tag="PLG52_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNB41204.1"
                     /translation="MNLYLIRHAIAEEESPAVEDRLRALTEKGSKKMRNIAKGLKTLG
                     LEFDYIISSPYVRAQQTAEILGDVFKLRKRIVISESIAPMGDPSQLLAEINEKYTVNS
                     LAIVGHEPYLSNLISLLTAGGAPLEMTFKKGGVCYLTTDDLHHTRKATLQWLLTPGVL
                     VEIGEG"
     gene            complement(3879..>4101)
                     /locus_tag="PLG52_06875"
     CDS             complement(3879..>4101)
                     /locus_tag="PLG52_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41205.1"
                     /translation="FHPPVAVRRVDEGRRRRKMKKNYARLCLCKNSRRLREASIVKTV
                     NKERNKKRAKYVSMPVSYNSHLILDLRTS"
BASE COUNT         1120 a          938 c          925 g         1118 t
ORIGIN      
        1 tgtaaagaac aagtaggtct tatctgatgt ccctgtttgg tcaataactg gtcttagcac
       61 aaagaaccaa agtgccattc cgattgaaat aaccacagtt gccgtctcga ggtaaatttt
      121 gagcttttca agagttgtct tactggtgta tggaaaactt aagatcccga tcaacatcag
      181 cggataagat aagaggtaaa agatatctgc cacagatgga tatggataaa tatgtaaaaa
      241 atgtgacata ccaaaccaaa acagattccc caaaaagcta aagagggcag aggttgcaat
      301 aatcgtccat gcccgcccta attgtttgga ggttggtcgg tagcgagccg ccacataaaa
      361 aagaagatag gtctcaccag cactccaaat gcaagcgaac aaatcaccat aaaaaggata
      421 aatggttttg gagcggatta agagaggggg gaaaataatt aacaacagca agaaaacata
      481 gggcgcatat gttttttgga cgggtctttt aaggaattca aacctgaggt ttaaagcttt
      541 aggaaaaaag gaaatatctt tctgtgtcat gtgaatctca tttcatatct gagcaactac
      601 ttaaattcaa acatgactcc ccaaacattc ctagtaggga aaacatctat ttcggagtaa
      661 atcggtctgt atatttcccc cgttacattg agctagtacc cgagcaaaac cgcctcagca
      721 gattcaagat tggtatagac agggatgatg gcaggaatat ttgtcatttc aagcacttcc
      781 atgacggctt gggtagggcg caaaataatg atcttgccat ttcggctctt aagggatttg
      841 gcattggtca ccaacaaacg aatgccaata gaagccaaat agtccacctt tgccatatcg
      901 acgatcacac gtgcattttc accggagcaa tatgccgcaa attttgtttc gatctgattt
      961 acaccggtaa tatctagcct gccgtctaat ttgattagtg taataccctt atctaaatcg
     1021 ctgacgttta attccataga agtcaccttt attttgaaga tggttcatta tatgaacgtt
     1081 aacattattg attgttaaca aaaggttatg gtttctactc acccatcaaa tgagattgga
     1141 catcgaaacg cttcccatta gaatctggtt tggaacgctc ataggtaccg ttcgttttta
     1201 gaatgcgcgc ccgggtatta tctttgagat aggcttccag aatcttatcg cgcaagaatt
     1261 tgacttgtcc cttattttca actgggaaaa ctacttcgac acggtggtta aggttgcgag
     1321 gcataagatc agcactaccc atataaattt catcattgcc attgttttgg aagtaataaa
     1381 tacggctatg ttcaaggtaa cgcccgacaa cacttagcac acggatattt tcactgacgc
     1441 ccttaatacc ggggcgcaaa cagcacatgc cgcgaatgag caaatccact ttgacgccag
     1501 cctgagaagc ctgatagaga agctggatca tatccatatc aaccagtgaa ttcatcttga
     1561 aaataatgcg tgccttgttt ccggctttcg cgtgttcgat ctcacgtcga atcagtttct
     1621 ccatctgctc acgcaaattg accggggcaa ccaacaattt cttataactt ttcttggtgg
     1681 agtaaccagt gaggtagtta aacaagtctg tggcatcggc gcccatatct tcatcgcaag
     1741 tgaacatgcc caaatcttca taaatgcggg atgtgaccgc attgtaattg ccggtggcaa
     1801 gatgcaaata tctgcggatg ccatcccctt ctttgcggac aaccatggct actttacagt
     1861 gggtcttcaa ccctactaag ccatagacaa catgaactcc tgcctgctcc aaggctcgcg
     1921 cccaaccaat atttgattct tcatcaaagc gggctttcaa ctccacgagt acggcaactt
     1981 gtttaccgcg ttctgctgct tctagcagag cttcaaccac aggcgcattg gaccctaccc
     2041 gatacaccgt ctgtttgatg gcaagcacat gcggatcgcg cgcggcggca taaagaaaat
     2101 ccacgactgg gatgaacgat tcgtaagggt gatgaagcaa aacattttca ttccgaatcg
     2161 catcaaatat ctctgaagat gtatccagat ttcggagtgg cttgggcgtg cgttgaatat
     2221 aaggcggata tttcaagtca tgccgttcga cgctgttata caactgccaa agactcgcca
     2281 acccaagcgg atgcggcaat gaataaatat catttcgggt aatttcaagg ttgtccacca
     2341 acaagtcacg catttcctct ggcatggacg ggtgaactgc tacctgtacc accgagccga
     2401 acttgcgttt gcgaatgttc tgttgcatgg tctctaacaa gtcatcggct tcgagttctt
     2461 ggatttcaat atctgcatcg cggacaatgc ggaagggatg tgcagagacc acttccaagc
     2521 caggaaaaag cccatcgaga ttggcagcaa tgacttggtc taaccaaacg aaataatggt
     2581 ggaaggggat cgtcccatct ttgcgggcgc tgccagaaga ttttttgatg gggatcaagc
     2641 gcggtaaagt atcaggcact ttgaggcgtg caaatttttc attgcccttt ttatcgcgga
     2701 tgacgatggc gagattaagg ctcaggttgg aaatatgcgg gaatgggtgt ccaggatcaa
     2761 gcgcgagcgg agtcagaacc ggatagacaa catccttgaa gtactgatcg gcgcgttcct
     2821 tctgagattt gctgagcttg gaataatcca gaacgtgaat gccttctttt tcaagcttgg
     2881 gcaacaactt gcgatggaag gtctgttggg catcttgcat caggtcttga gctgttttgc
     2941 gaatgcctgc cagttcctca cgcggagtta gcccatcttc agaaagcttc atgatgttgg
     3001 cttccacttg tttgcggatg ccagagacac gcaccataaa gaactcgtcc atattcgagc
     3061 cgaggattgc cagaaacttc acgcgttcaa gcagcggatt ttcttcatcg cgcgcttctt
     3121 ctaggacgcg gcgttggaac tctaataggc ttgcttcacg gttgatatat aaagaaggat
     3181 ggctcaggtc gatctcggtc attaggtttt cctcaaggaa ggatgggcgt gcaaaagaat
     3241 ccctgtattc tattatcctt ccccaatttc aaccagcaca ccgggtgtta atagccattg
     3301 cagggttgct ttgcgggtgt gatgtaagtc atctgtcgta aggtagcaaa ccccgccttt
     3361 cttaaaggtc atttcaagag gagcgccacc agccgttagt aaactgatga gattcgacag
     3421 atacggctca tgcccaacga ttgcaaggct gttgaccgta tatttttcat tgatctccgc
     3481 caataactgg ctcgggtctc ccataggagc aatgctttcg ctaatcacaa tgcgcttcct
     3541 aagtttgaag acatcgccca aaatctctgc cgtttgttga gcgcggacgt atgggctgct
     3601 aatgatgtag tcaaactcca gcccaagggt cttaagcccc ttggcgatgt tacgcatctt
     3661 tttggagcct ttctcggtca acgcgcgcag acggtcttct accgcaggcg attcttcttc
     3721 ggcaatggca tggcggatca gatataaatt catagggcgc tctcctgaaa ataaagtact
     3781 cgaattatac aggctgcaaa attttcctca ttaagaatat gtaaataaat ctgtacaggt
     3841 ggttttcgcc tccgccttgc ataatcgttt acccgttgtt aactggttct taagtccaaa
     3901 atcaaatgac tgttgtaaga tacaggcatg gaaacgtact tcgctctttt cttatttctc
     3961 tctttgttga ccgtcttcac aatagacgct tctcgcaaac gtcgcgagtt tttgcaaaga
     4021 caaagccgcg cgtagttctt tttcattttt cttctcctcc ttccttcatc cacccgcctg
     4081 acagcgacag gcgggtggaa t
//
LOCUS       DAOQNJ010000196         4096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_13969_length_4096_cov_9.498391, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000196 DAOQNJ010000000
VERSION     DAOQNJ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13969_length_4096_cov_9.498391"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PLG52_06880"
     CDS             complement(<1..1288)
                     /locus_tag="PLG52_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB41206.1"
                     /translation="MLSALLDFWKRDEETSPNIFAWQTLPPREAQTHPIPDDVPASLR
                     EALSTRGISQLYSHQQSAWTHARARRNVILATGTASGKTLAYNLPVLAKLIEDTQARA
                     IYLFPTKALTQDQQSSLESFQSSIANLKSSIYDGDTPQPQRSTIRKTARIILTNPDML
                     HTGILPHHSNWGDFFTNLKFIVIDEAHTYRGVFGSHVANVIRRLRRVAKFYGANPQFI
                     LASATIGNPKELAETLIEEPVELVDNDGSSRGQRHFIIYNPPVLNPALGLRKSSILES
                     VRLANDLLKHDVQSVVFARTRRSVEILLTHLQTQIVNLKSKIENPIRGYRSGYLPTQR
                     REIEQGLRDGSVKTVVATNALELGIDIGGMGAALMVGYPGTIASARQQAGRAGRGDLP
                     AVSILVASPNPLDQFLAHHPEYFFDRSPEMALVNPNH"
     gene            complement(1294..2304)
                     /locus_tag="PLG52_06885"
     CDS             complement(1294..2304)
                     /locus_tag="PLG52_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB41207.1"
                     /translation="MNKFLVFLFLFLGVVVVVFSFGELETILQTLQKAHLGFFLLAVL
                     IQIAWFVSSGRMYQSIFHLLGIHEDVLTLSRIAAAANFINVVAPTAGAGGVALFAAEA
                     KRRGHPAGKATVAAALFLLLDLAAFLIILSLGLIVLVRRNDLDAGEISASLILFTTAA
                     GFTSVLYIGYRSAEKLGNILAKLARGINKVVHLFRKENYLSEERAHEFAHELADGFSG
                     LTEQPSSLIRPVLWGLFEKGLLMLILMCSFLAFEVSFSAGTIIAGFAIAYLFLIVSPT
                     PSGIGIVEGIMPVALSSLNVNWSQGVVITLLYRAVTFWVPLGIGAWAFRTLHKERAEV
                     AE"
     gene            complement(2451..3143)
                     /gene="cysE"
                     /locus_tag="PLG52_06890"
     CDS             complement(2451..3143)
                     /gene="cysE"
                     /locus_tag="PLG52_06890"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNB41208.1"
                     /translation="MFKTIREDVQSVLDRDPAARNVFEVLLCYPGVHAIWYHRLSHKL
                     WKGGFKLLARWVSQFARGLTGVEIHPGAKIGKNFFIDHGMGVVIGETAEVGHNVTLYH
                     NVTLGGTSSEKVKRHPTLEDNVVVGAGAKVLGAIVIGEGSRIGANAVVVKSAPPNSVI
                     VGVPGQVLLRSHTNKQIDLNHNLLPDTIGDTVAALVAHVNELEKKMNTHFMGSPLHRS
                     EHGVWHGEDFAI"
     gene            complement(3200..>4096)
                     /gene="cysK"
                     /locus_tag="PLG52_06895"
     CDS             complement(3200..>4096)
                     /gene="cysK"
                     /locus_tag="PLG52_06895"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNB41209.1"
                     /translation="PLVRLNRVTEGAKAEVVAKLEFFNPAHSVKDRIGFSMIDAAEKA
                     GLINKDTVLVEPTSGNTGIALAMVAAARGYKLILTMPETMSKERRMLLRAYGAELVLT
                     PGPDGMGGAIRKAEEIVASDPKKYFLPQQFKNPANPEVHRKTTAEEIWRDTDGKVDYL
                     VAGIGTGGTITGVGEIIKERKPSFKAIAVEPDASPVLSGGAKGPHPIQGIGAGFVPDI
                     LNTKIYDEIIRVKADDAFQTARRLAREEGLLVGISSGAATWAALQVANRAENAGKLIV
                     VIIPSFGERYLSTALFADLVDN"
BASE COUNT         1076 a         1012 c         1059 g          949 t
ORIGIN      
        1 aatgattcgg gttgaccaat gccatctctg gcgaacgatc aaaaaagtat tcgggatgat
       61 gcgcgaggaa ttgatccaac ggattcggtg aagcaacaag gatcgaaacc gcaggcaggt
      121 cgccacgtcc cgctcgccct gcttgctggc gcgcacttgc aatggtgccg gggtagccaa
      181 ccatgagggc agcccccatg ccgccaatgt caatgccgag ttcgagcgca ttggttgcca
      241 caacggtctt gaccgagcca tcacgaagtc cctgctcgat ctcgcggcgt tgagttggga
      301 ggtagccaga gcgatagccg cgaatcgggt tttcgatttt cgatttcaga tttacgattt
      361 gtgtttgtag atgggttaga aggatttcca cactacggcg tgtccgcgca aagacgaccg
      421 actgcacatc atgtttcagc aaatcattcg ccaagcgtac cgattcaagg atgctgctct
      481 tgcgcaagcc caatgcagga tttaacacag gcgggttata gataatgaaa tgacgctgtc
      541 cgcgagagga accgtcgtta tccaccaatt caacgggttc ttcaataagc gtctcggcta
      601 attcttttgg gttgccaatg gttgccgaag caaggatgaa ctgcggattc gcgccataga
      661 attttgccac ccgtctgagc ctgcggatga cgttggcgac atgcgaaccg aagactccgc
      721 ggtaggtatg ggcttcgtcg atgacgatga attttaggtt ggtgaaaaag tcgccccagt
      781 tggaatgatg cggcagaatg cccgtatgca gcatatctgg attcgtgaga atgatgcgtg
      841 cagttttgcg aatggtggag cgttgtggtt gaggagtgtc gccgtcatag attgacgatt
      901 tgagatttgc gattgacgat tgaaaagact cgaggctcga ttgttgatct tgagtaaggg
      961 ctttggtggg gaagagatag attgcgcgtg cttgggtgtc ttcgatcaac tttgcgagga
     1021 caggcaagtt gtatgcgaga gtcttgccac tggcggtgcc ggtggcgaga atcacatttc
     1081 ggcgggcgcg ggcatgagtc cacgcggact gttggtggga atagagttgg gaaatgccac
     1141 gggtagataa agcttctcga agcgaggcag gcacatcgtc tggaatggga tgcgtttgag
     1201 cttcacgtgg cggaagcgtt tgccaggcaa agatattggg tgaggtttct tcgtcccgct
     1261 tccaaaaatc cagaagagca gagagcatga acgttactcg gcaacctcag cgcgttcttt
     1321 atgcaaggtg cgaaatgccc aagcaccaat tcccaatggc acccaaaatg tgaccgcgcg
     1381 atagagcagt gtgatgacca cgccctgact ccagttaacg ttcaaagagc ttaaggcaac
     1441 cggcatgatg ccttcgacaa taccaatgcc agatggggta ggcgagacga taagaaagag
     1501 gtatgcaatg gcaaaaccag caatgatggt gccagctgaa aatgaaactt cgaaggcaag
     1561 gaatgagcac ataagaatca acatcaacaa acctttttca aacaagcccc acaacactgg
     1621 gcgaataagg ctggagggtt gttctgttaa ccctgaaaac ccatcggcaa gttcgtgtgc
     1681 aaattcatgg gctcgctctt cgctaagata attttctttt cggaaaagat gtacgacctt
     1741 gttgatgcca cgcgcaagtt tcgcaagaat gttccccaat ttttcagcag agcgataccc
     1801 gatgtataaa acagatgtaa agcctgcggc tgttgtaaat aaaatcagag aggctgagat
     1861 ctctccggca tccaagtcat ttcggcgaac aagcacgatc aagccaagtg agaggatgat
     1921 caagaaagct gccaggtcta ataggaggaa caatgcagct gcgacagtcg ctttgccagc
     1981 aggatgccca cgccgtttcg cttcggcagc aaagagcgcg actcctcccg cgccggcagt
     2041 gggtgccacg acattgataa aattcgccgc tgcggcaatg cggctgagag tgagcacatc
     2101 ttcatgaatc cccaatagat gaaagatgga ttgatacatc cgcccgctgg aaacaaacca
     2161 agcgatctga ataagaactg ccaatagaaa aaagccaagg tgtgcttttt ggagagtctg
     2221 aagaatcgtc tccaactcgc caaagctgaa aacgaccaca actacgccca gaaaaaggaa
     2281 caagaaaacc agaaatttat tcatgtatac ctatatacct acacagacat tgtaacaaaa
     2341 cattgaccct ttctgtacgg gagcaaaaga ttcatgacag ccataaaaat agaccgttgc
     2401 cgcgtgtttt atacgcagta acggtctatt atgttgcgga agatcaaaac ctaaatggca
     2461 aaatcttcgc cgtgccatac tccatgctca gatcgatgca aaggagaccc cataaagtgt
     2521 gtgttcattt tcttttccaa ttcgttcaca tgcgcaacca aagccgcaac tgtatcaccg
     2581 atggtatctg gcaataagtt gtggttgagg tcgatctgtt tgttggtatg gcttcgcaaa
     2641 aggacctgcc ccggaacccc aacaatgacc gaattaggcg gcgcagactt aacaacaaca
     2701 gcatttgcgc caatacggct gccttcacca ataacgatcg cgcccaaaac ttttgcacca
     2761 gcgccaacga ctacattatc ttccaatgtc gggtggcgtt tcactttttc agaactggtg
     2821 ccgcccagag tcacgttgtg atacaaggtg acattatgcc ctacttcagc cgtctcacca
     2881 atcacaacgc ccatgccgtg atcgataaag aagttcttac cgatctttgc gccagggtga
     2941 atctctacac cggtcagtcc gcgtgcgaat tgagagaccc agcgtgcaag cagtttgaaa
     3001 ccgcctttcc aaagcttgtg tgaaagacgg tgataccaaa tggcatgcac gcccgggtaa
     3061 cacaatagca cctcgaacac attgcgggct gcggggtcac ggtccagcac agattgaaca
     3121 tcttcacgaa ttgtcttgaa cattgaaact cactttacga gaaaaccatc acgctctttt
     3181 aattgagcgc tttaaaatgt tagttatcga ccaagtctgc aaacaaagcg gtggacaagt
     3241 accgttcccc aaacgaaggg atgatgacca cgatcaattt gcctgcattt tcagcgcggt
     3301 tggcaacctg aagcgcagcc catgtcgctg cacctgaaga aatccctacc agcaaccctt
     3361 cttcacgtgc caagcggcga gcggtctgaa aagcatcatc tgctttgaca cgaatgatct
     3421 catcataaat tttggtattg agaatatcgg gcacaaaccc ggcaccgatc ccttgaatcg
     3481 gatgcggtcc tttggcgccg ccagaaagaa cgggggaggc atctggctct acggcgatgg
     3541 ctttgaagga aggtttgcgt tctttgatga tctcgcccac accggtgatg gttccacctg
     3601 tgccaatgcc agccacaaga taatctactt tgccgtctgt gtcgcgccag atctcttccg
     3661 cggtggtctt gcggtgaact tcagggttgg caggattctt aaattgctgc ggcagaaaat
     3721 acttcttggg gtctgaggcg acaatttcct ccgccttgcg aatcgcaccc cccatgccat
     3781 cgggcccggg ggtgagaacc aactctgctc catacgctcg caacaacatg cggcgctcct
     3841 tgctcatggt ttcgggcatg gtgagaatta gtttgtatcc acgtgctgcc gcgaccattg
     3901 ccaaggcaat gcctgtgttt ccagatgtgg gttctacgag aaccgtatct ttattaataa
     3961 gacctgcctt ttcagcagca tcgatcatcg agaaaccgat gcggtccttt acgctatggg
     4021 ctgggttgaa aaactcgagc tttgcaacca cttctgcttt ggctccttcg gtaacgcgat
     4081 tcaagcgcac caatgg
//
LOCUS       DAOQNJ010000197         4090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14009_length_4090_cov_7.194796, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000197 DAOQNJ010000000
VERSION     DAOQNJ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14009_length_4090_cov_7.194796"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLG52_06900"
     CDS             complement(<1..315)
                     /locus_tag="PLG52_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41210.1"
                     /translation="MPQQFAEKYLLALEESIKEKPKGGLAGFEQEWNLLDNDLRPLLT
                     VGAGPSQHSFVDYLRAECIPVWQKQFSQLEVFHWMVEWATRPYYSPRGAIYEARLMEA
                     SLM"
     gene            complement(401..1705)
                     /locus_tag="PLG52_06905"
     CDS             complement(401..1705)
                     /locus_tag="PLG52_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB41211.1"
                     /translation="MSVSNTTPVLAVDEKHLVPVSDHLKQMMDIPPSRMFLINKSLKV
                     YQEKNAGGKIFDASQGDGGASLPGTPKPILERALQLQLEHGTAYDMPFGTEAYRKAVI
                     EKYWKLDASSGWGPANVLGTAGGRDALNKAYQAMLALGYGRQGDLIMVSRVPWISYNW
                     GPYGVGANVLWTPGDPAQGWAYSEEAIRESVKFAESKGRKIAGIVITNPDNPTGMTIA
                     VEKQVSLAKAALEAGVAFVLFDWMYHYVTDESPMDLNSFLKAFTPEERKRIMFLDGIT
                     KSLGGSNIRNCHLIADEAVIKFITARASHGVIPSFFSLAVAMAAYEMGFNEAAKTIIE
                     PTNASRVALKKLLNESGLQHIIGKGYYAFMNVGEFIKAKGWADTEPLGQYLAENHGVA
                     VVPGAFFSPFGGEWIRFSYATPVERTEGAFKRLMEGLNELKK"
     gene            complement(1929..2213)
                     /locus_tag="PLG52_06910"
     CDS             complement(1929..2213)
                     /locus_tag="PLG52_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNB41212.1"
                     /translation="MLPYHLQNLARTGREVQIAVDGAPTIGSVLEALEKQYPMLRGTI
                     RDQATGQRRPFIRFFVCGADWSLEAWDVELPLAIQNGSEVFRVVGAMSGG"
     gene            complement(2222..3334)
                     /locus_tag="PLG52_06915"
     CDS             complement(2222..3334)
                     /locus_tag="PLG52_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41213.1"
                     /translation="MSGVRVLVGTRKGAFILTSDAKRKKWKVSGPHFAGWEIYHMHGS
                     SVDPNRLYASQSSGWFGQVIQRSNDGGESWEPVGNQFAYHGTPGTHMWFDGTQHPWEF
                     KRVWRLEPSLTDVDTVYAGVEDAALFRSTDGGQTWTELAGLRDVKGHLWQPGAGGMCL
                     HTIVLDPTDPKRMFIAISAAGAFRTENGGETWLPINQGLSSGELPDADAEVGHCVHNI
                     SMHPTRPNVLFMQKHWDVMRSNDAGASWHEVSGNLPSDFGFPIAVHAHEPETIYVVPI
                     KSDSEHFPPDGKLRVYRSRTGGNDWEALTKGLPQENCYVNILRSAMAVDTLDSCGIYF
                     GTTGGQVYASSDSGDSWTPIVSNLPAVLSVEVQTLK"
     gene            complement(3385..3669)
                     /locus_tag="PLG52_06920"
     CDS             complement(3385..3669)
                     /locus_tag="PLG52_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNB41214.1"
                     /translation="MTGTLTAQIGKRELLVNILVRASALVMTMPGCKAYIVLEDLKDE
                     QTVAVFEMWDDKEAHDVSLQDPRVRSLISEAMPILAGAPSGGEYRVATNL"
     gene            complement(3682..4044)
                     /locus_tag="PLG52_06925"
     CDS             complement(3682..4044)
                     /locus_tag="PLG52_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB41215.1"
                     /translation="MSKRNIVHVEIPAVNTAAAGEFYQELFGWKTTPMPEMNYVMWEA
                     GSGDEYGGFPEVSAENPAGQVLVYIASDDIDEDLEKVSQLGGKVLREKTEIPGMGWYG
                     VFQDPTGNVLALYTGMNS"
BASE COUNT         1037 a         1077 c          987 g          989 t
ORIGIN      
        1 catcaaagag gcttccatca ggcgggcttc gtaaatcgca ccacgcggag agtaatacgg
       61 gcgggttgcc cattccacca tccagtgaaa gacttcgagc tgactgaatt gcttttgcca
      121 aacaggaatg cattcagcac gcaaataatc gacaaaagaa tgttggctgg gtccagcacc
      181 gacagttaac agtgggcgca ggtcgttatc aagcagattc cattcctgtt caaacccagc
      241 gagaccgcct tttggttttt ctttaatgga ttcttcaagg gcaagtagat atttttctgc
      301 aaattgttgt ggcatggata tttcctaaga taaattggaa taatggctat aaaacagaaa
      361 gtagaaagtg aggacgaaca tcactttcta ctttctgtca ctacttcttc aattcattca
      421 agccttccat taagcgtttg aatgcgcctt cggtacgctc aacaggtgtg gcatacgaga
      481 agcggatcca ttcgccgccg aagggtgaga aaaatgcacc cggaacgacc gcaacgccgt
      541 gattttcagc aaggtactga cccaaaggct cggtatctgc ccagcctttg gctttgataa
      601 attcgcccac gttcataaag gcataatagc ctttaccgat aatgtgttga agtccgcttt
      661 cgttgagaag tttcttgagg gcaactcggc tggcattggt cggttcaatg atggtcttgg
      721 cagcttcgtt gaaacccatt tcgtaggcag ccatggcaac cgcaagcgag aagaacgaag
      781 ggatgacgcc atgggaagca cgagcggtga tgaacttgat aacggcttca tctgcaatga
      841 ggtgacagtt gcggatgttt gaaccgccca aggatttggt gattccgtcc aggaacatga
      901 tgcgtttgcg ctcttcgggg gtgaatgcct taaggaaaga attcaaatcc attggcgatt
      961 catcggtgac gtagtgatac atccaatcga agagtacgaa ggcaacgccc gcttcgaggg
     1021 cagccttggc aagcgagacc tgcttttcaa cggcaatggt cataccggtg gggttatcag
     1081 ggttggtaat gacaatgccc gcgatcttgc gtccttttga ttcggcaaat ttgacgcttt
     1141 cacgaatcgc ttcttcagag tatgcccaac cttgtgccgg gtcgccggga gtccacaaga
     1201 cattggcgcc cacaccgtat ggaccccagt tatatgaaat ccacggcaca cgtgagacca
     1261 taataaggtc accttggcga ccatagccga gggcaagcat ggcttgataa gctttattga
     1321 gggcatcacg tccgccggcg gtgccgagca cattggcagg tccccatcca gaagaggcat
     1381 ctagcttcca atatttttca atgaccgcct tacgataggc ttcagtgccg aacggcatat
     1441 cgtatgccgt accatgctcg agctggagtt gaagcgcgcg ttccagaatc ggctttgggg
     1501 ttccgggcaa cgaagcgccg ccgtcgcctt gagaagcatc aaagatcttc cctccggcat
     1561 tcttttcctg gtagaccttt aacgatttat tgatgagaaa catacgggaa ggcggaatat
     1621 ccatcatctg cttgagatga tcgctgacag gcacgagatg tttttcgtca acagcaagaa
     1681 caggggtggt attggaaacg gacatggtga attctcctta agaaaaatgc cccgaggctc
     1741 ggggcatttg gtgtacttag taagattgaa cttgttgctg ttcacctgac aaccaccaat
     1801 gccccgggca ccaacgattg gtgcgggtag tattggtatt ggtgtcaaca acgaattgct
     1861 tgaacattgc gcgcaagcat accacttgca tatacatgcg tcaagcagga atttaggttt
     1921 gcagtaactt agccgccaga catggcgcct accacacgga acacttcgct cccattttga
     1981 atcgcaagcg ggagctcaac atcccaagcc tccaaggacc agtcggcacc acaaacaaaa
     2041 aagcggatga aaggtctacg ctgacccgtg gcttgatcgc gaatggtgcc gcgcagcatg
     2101 ggatattgtt tttccaacgc ttccaacacg gacccgatcg taggcgcccc atcgacagct
     2161 atctgcacct ctctgcctgt gcgcgccaga ttttgcagat gataaggaag cacaacccgt
     2221 atcatttcaa ggtttgcact tccacagaca acacggctgg gaggttactg acaatgggcg
     2281 tccaactatc gccagagtct gaagaggcgt acacttgccc accggttgtg ccaaaataaa
     2341 ttccacaaga gtcgagcgta tctactgcca tggcagagcg caaaatgttg acgtaacagt
     2401 tttcctgcgg caaacctttc gtcaacgctt cccaatcatt accgccggtg cggctacgat
     2461 atacacgcag tttcccatcc ggtgggaagt gttctgaatc acttttaatt ggtactacat
     2521 aaatggtctc tggctcatgg gcatggactg cgatcgggaa gccaaaatcg ctgggcaaat
     2581 ttccgctcac ttcatgccat gaagcgccag catcattgct gcgcatcaca tcccaatgtt
     2641 tttgcatgaa caagacattc gggcgtgttg ggtgcatgga aatattatgt acacagtgac
     2701 ccacctccgc atcagcatca ggcaattcgc ccgaagacaa tccctgattg atcggcaacc
     2761 acgtctcgcc gccattttca gtgcggaaag cccctgcggc agaaatagcg ataaacatac
     2821 gctttggatc agtcgggtca agcacaatgg tgtgcaagca cattccacct gcgccgggtt
     2881 gccagagatg acctttcacg tctcgcagtc cggctaactc ggtccacgtc tgcccgccat
     2941 cagtggagcg gaataaagct gcatcttcca ccccggcgta gacggtgtcc acgtcggtca
     3001 aagaaggttc caagcgccaa acacgcttaa attcccaggg atgttgtgtg ccatcgaacc
     3061 acatatgggt gccgggcgtg ccatgatacg caaattgatt tccaaccggc tcccacgatt
     3121 cgccgccatc atttgaacgc tgaatgacct gcccaaacca accgctagac tgcgaagcat
     3181 acaagcggtt cgggtccaca gaagagccgt gcatgtggta gatctcccag cctgcaaaat
     3241 gcggaccgct caccttccat tttttacgtt tggcatctga ggtcaaaata aatgcccctt
     3301 tacgggttcc aactaatact cgtactccgc tcatcacgct ctccttggtc aataaatagt
     3361 aacgacttaa gcttgctctt tcagttacag gtttgttgct acacggtatt cgccaccact
     3421 cggtgccccg gcaaggatgg gcattgcctc agagattagg cttcgtacac gagggtcttg
     3481 taaagataca tcgtgagcct ctttatcgtc ccacatttca aacacggcaa cggtttgctc
     3541 gtcttttagg tcttccagca caatataggc tttgcagccc ggcatggtca taaccaatgc
     3601 cgaagcacgc acaaggatgt tcactaaaag ctcacgcttc ccaatttgag ccgtgagggt
     3661 tccggtcata gcgtacataa attaagaatt catcccagta tataaagcca gaacattccc
     3721 agttgggtct tggaacaccc cataccaacc catgcccgga atctcggtct tttcgcgcag
     3781 tactttgccg cccaattggc tgaccttttc caaatcttcg tcaatatcgt cactggcaat
     3841 gtagaccagc acctgcccag cagggttctc ggcagaaact tcagggaagc caccatattc
     3901 atctccactg ccggcttccc acatcacata gttcatttcg ggcatgggcg tggtcttcca
     3961 gccgaaaagt tcttgataga actcgcctgc cgccgcagta ttcacagcag gaatctcaac
     4021 atggacaata tttcgtttgg acatgacatt ctccttaatt cgatacttat tatccattat
     4081 agaacatttt
//
LOCUS       DAOQNJ010000198         4089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14014_length_4089_cov_10.400347, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000198 DAOQNJ010000000
VERSION     DAOQNJ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14014_length_4089_cov_10.400347"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PLG52_06930"
     CDS             <1..990
                     /locus_tag="PLG52_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNB41216.1"
                     /translation="TEVHNAEELPDKTWRITLGNGKVEIFDFLLIANGHHWNPRYPKY
                     EGEFTGEFVHSHEYKRAAPFAGKRVLVIGAGNSGCDISVETARVSAFTAMSMRRGQYI
                     VPKFVFGQPPDVVNGRFYWLPAWIRLPFFYLTLKLAVGKYEDYGLPKPKHGILNAHVT
                     NNSEILYAIRHGKVHVRPDIKRFNGKQVEFVDGRVEEYDSIIAATGFKISLPFFDPNL
                     LSIEEGVPPLYKNVFHPDHPSLFFIGLVQPLGSIWPLSDLHAKLAANYIIGNYHLPKD
                     IRQRIARHIEYMKKHYVNSPRHSVEVEFHPHIVELKREIPKSAPEWKHGYSDR"
     gene            971..1639
                     /locus_tag="PLG52_06935"
     CDS             971..1639
                     /locus_tag="PLG52_06935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNB41217.1"
                     /translation="MDIQIDNLVRAKRRTIALIVERDGSLTVRAPRRATLNDIYGFIQ
                     EKTDWILRSREKLKAIKPIPKKAYVDGERFLFLGQEYELRLVPPQRPALKFDGGFTLS
                     TSAQERGELAFTKWYKTQALTLFTERVNYYANLHGLIPKEVKVNSAKTRWGSCTSAGT
                     LNFTWRLVMAPLEVIDYVVLHELAHLKVKNHSPRFWKLVESLCPDFKHHRKWLRDHGE
                     TMTL"
     gene            1660..2082
                     /locus_tag="PLG52_06940"
     CDS             1660..2082
                     /locus_tag="PLG52_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41218.1"
                     /translation="MLPETLIEVHEHTEPDYKPLVDYQSWRVALMNYTDDLIPEKINR
                     MQKHTETDEVFVLMAGHCILFLGEGEETVTKVHAVDMELYKLYNVKRGVWHSHTFSKD
                     ARILIVENRDTVVENSPFVDLSPSQCTEVLELTKQLWK"
     gene            complement(2117..2965)
                     /locus_tag="PLG52_06945"
     CDS             complement(2117..2965)
                     /locus_tag="PLG52_06945"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNB41219.1"
                     /translation="MKAELFFDAKSNLGEGPSWDAKTQTLYWVDIFEKRVYAGNEILA
                     QFDEYVGFVAPTKSGHLIVGKRASFVDLDIATSQQTVLVTLNESATNRMNDGKCDPSG
                     RLIAGTMDLNETDPTGSVYSYDGKSTRVLFRDVTISNGMAWSPDYKTFYYIDTPACEV
                     RAYDYDIKTGEIANRRRAFEVPKSLGWADGMTSDVEGNLWIAMWGGAQVTRWNPNTGQ
                     LLEQVSVPALHSSCPVFGGKNRNELYITSARKGMSEADLNKYPLSGGLFKVDTKYEGM
                     PTFEFG"
     gene            complement(2965..3891)
                     /locus_tag="PLG52_06950"
     CDS             complement(2965..3891)
                     /locus_tag="PLG52_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNB41220.1"
                     /translation="MLSIYKTTENGLEKLDAIANGTWVNVIDPTPDEMEKLVGWGMEM
                     DYINYSLDQDEMARMERDEDYTFILLRIPIHQPESDIPYNTVPLGIMILGNKIITVCR
                     FDSDIFRALTNGKHRYLKTGKRYRLTLYIFLETAVRYLNLLREINRATEAVEDRLQKS
                     TQNREVLELLKYQKSLTYFATALRSNEVMMERVQKTQLFNYYEEDQDLLEDVLTENQQ
                     AIQMTSIATEILSGMMDAFASIISNNLNVVMKALAALTIILNMPTIVASFYGMNVDLP
                     GEGHPLAFLTVIGLSLALTALATYIFYKRNWF"
BASE COUNT         1109 a         1020 c          928 g         1032 t
ORIGIN      
        1 accgaagtcc acaacgctga agaattacca gataaaactt ggcgcatcac acttggaaat
       61 ggcaaagtag aaatctttga ctttctactc atcgcgaatg gacatcattg gaatccacgt
      121 tatccaaagt atgaaggtga gttcacggga gaattcgtcc attcgcatga atacaaacgc
      181 gcagcaccat ttgcagggaa gcgtgtactc gtcattgggg cgggcaactc gggctgtgac
      241 atttcggtag aaaccgcacg tgtttcggcg ttcaccgcca tgtccatgcg acgcgggcaa
      301 tacatcgtgc ctaagttcgt ttttggtcag ccgcctgatg tggtcaatgg tcgtttttac
      361 tggctgcccg cgtggattcg cctgcccttc ttttatctaa ccctcaaact tgctgtcggt
      421 aaatacgaag attatggctt gcccaaaccc aaacacggca tcttgaacgc gcacgtgacc
      481 aacaactcag aaattctcta cgccatccgt cacgggaaag ttcacgtccg cccagatatc
      541 aaacgcttca acggcaaaca agtagagttt gtggatgggc gcgtggaaga atatgactcg
      601 atcattgcag ctaccggatt taagatctcg cttcctttct tcgacccgaa cctgctctcc
      661 atcgaagaag gtgttcctcc gctgtataag aatgttttcc atcccgacca cccatcgcta
      721 ttctttatcg gtctcgtgca accgctcggc tcgatctggc cactctcaga cctgcacgcc
      781 aaacttgccg ctaattatat tatcggcaat taccacctac ccaaagatat acgtcaacgc
      841 attgcgcgcc atattgaata tatgaaaaaa cattacgtca attcgccgcg ccacagcgtg
      901 gaagtggagt ttcatccgca cattgtggag ctaaagcgcg agattcccaa gtctgcgccg
      961 gaatggaagc atggatattc agatcgataa ccttgtgcgt gccaaacgcc gcaccattgc
     1021 cctcatcgtc gaacgagatg gtagccttac cgtccgtgcg ccgaggcggg ctacactgaa
     1081 tgatatctat ggcttcatcc aagaaaaaac cgattggatc ctacgttcgc gcgaaaaact
     1141 aaaagcgata aagcccatcc cgaaaaaagc atatgtggat ggagaacgtt tcctctttct
     1201 tgggcaggag tacgaactcc ggctcgttcc acctcagcgg cctgccctca aattcgacgg
     1261 tggattcacc ctgtccactt ctgcacaaga acgcggtgaa ctagccttca ccaaatggta
     1321 caaaacccag gcattgaccc tcttcacgga acgtgtgaac tattacgcca acctacacgg
     1381 acttatcccg aaagaggtca aagtcaactc ggcaaagaca cgttggggtt cttgcacatc
     1441 tgccggcacg ctcaacttca cctggcgctt ggtcatggcg ccactcgagg tgatcgatta
     1501 tgtcgtgctc catgagttgg cgcatttgaa ggtcaaaaat cattccccgc gattttggaa
     1561 gttggtagaa tcactatgcc ctgactttaa acatcaccgc aagtggttac gcgaccacgg
     1621 cgaaacaatg accttataac ttttctctgg agaatagcca tgctgcccga aactctcatc
     1681 gaagtccacg aacataccga accagattac aaacctcttg tcgattatca gtcttggcga
     1741 gtggcactaa tgaattatac agatgaccta atccccgaga aaatcaatcg catgcaaaag
     1801 catacagaaa ccgatgaggt ctttgtgctt atggctgggc attgtatttt atttctcggc
     1861 gaaggcgaag agacggtaac caaagtccac gccgtggata tggagttgta caaactttat
     1921 aacgttaagc gtggcgtatg gcattcgcat accttcagca aagatgcccg cattttgatc
     1981 gtggagaatc gcgataccgt tgtggaaaat tcgccctttg tggatctatc gccaagccaa
     2041 tgcacagaag tgctggaatt aacaaaacaa ttatggaagt aaaacgtaag agcgaggaaa
     2101 ccccgccctt acgtttttac ccaaactcaa aagtgggcat gccctcatat tttgtgtcaa
     2161 ctttgaacaa cccgccagag agcgggtatt tatttaagtc cgcttcgctc atgcctttgc
     2221 gagcagaggt gatatacaac tcattacggt tcttgccacc aaagaccggg caggaagagt
     2281 gcaaagcagg cacagaaacc tgctccaaga gttgaccggt attcgggttc cagcgtgtga
     2341 cctgtgcccc accccacatg gcgatccata gattgccttc aacatcagag gtcatgccgt
     2401 ctgcccagcc aagggatttg ggaacctcaa aagcacgccg gcggttcgcg atttcaccgg
     2461 tcttgatatc ataatcatag gcgcggactt cgcaggcggg cgtatcaatg taatagaagg
     2521 ttttatagtc tgggctccat gccatgccat tggagatggt cacatcacgg aacaaaacac
     2581 gggtggattt tccatcatag gaatagacag agcctgtggg atccgtctcg ttcaaatcca
     2641 tggtgcctgc aatcaagcgc ccagatgggt cacatttgcc atcgttcata cggttggtgg
     2701 cagattcatt caaagtgaca agcacggtct gctgggaggt ggcaatgtcc aagtccacga
     2761 aagaggcgcg tttgcctacg atgagatgtc cgcttttagt aggggcaaca aatccaacat
     2821 attcatcaaa ttgagcgaga atttcgttac ctgcatagac tcttttttca aaaatatcaa
     2881 cccaatacaa ggtttgagtt tttgcatccc aagatggacc ttcgcccaag tttgattttg
     2941 cgtcaaaaaa aagttcagct ttcattagaa ccaattcctt ttatagaaga tataagtggc
     3001 caatgccgtg agcgccaacg acaagccaat aaccgttaag aatgccagcg ggtgtccttc
     3061 tcctggcaaa tccacattca tgccgtaaaa cgatgcaacg atggtgggca tattgagaat
     3121 aatcgtcagt gccgcaagcg ctttcatcac tacgtttaag ttattcgaga taatcgaagc
     3181 gaaggcatcc atcataccag agagaatttc agtagcgatg cttgtcatct gaatcgcttg
     3241 ctgattttcc gtgagcacat cttcgagcaa gtcttgatct tcttcataat aattgaagag
     3301 ttgagtcttt tgtacgcgtt ccatcatcac ttcgttagag cgcaacgcgg tcgcaaagta
     3361 ggtaagactc ttttgatact taagcaattc aagcacttcg cgattctggg tagatttttg
     3421 caagcggtct tccacggctt cggtagcgcg attgatctca cgcaacaagt tcaaataacg
     3481 cacagccgtt tcgagaaaga tataaagcgt gaggcgataa cgcttgccag ttttcaaata
     3541 gcgatgtttg ccattggtta atgcgcgaaa gatatcacta tcgaagcggc aaactgtgat
     3601 gatcttattg ccaagaatca taatgccaag cggaacggtg ttataaggaa tatcgctctc
     3661 tggttggtga atgggaatgc gaagaaggat gaaggtgtaa tcttcatcgc gttccatacg
     3721 cgccatttca tcttgatcaa gtgaataatt gatatagtcc atctccatcc cccaacctac
     3781 taacttttcc atttcatccg gcgttgggtc aatcacatta acccacgttc cattcgcgat
     3841 cgcatcgagc ttctctagac cgttctcggt cgttttgtag atactcagca taacagcctc
     3901 ctacgtggaa tcatcacgta agggcatggc acctacgtga aaggtattaa aacgttaaat
     3961 acatgttcac cgctacttag cgggttacta tctgaagaac ctacatcgct ttcagtggtt
     4021 agatacatac ttttgaaccg cgccaatttt aagccggatg atggaattac acaacagttt
     4081 caggatttg
//
LOCUS       DAOQNJ010000199         4084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14043_length_4084_cov_9.103251, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000199 DAOQNJ010000000
VERSION     DAOQNJ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14043_length_4084_cov_9.103251"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="PLG52_06955"
     CDS             <1..1880
                     /locus_tag="PLG52_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNB41221.1"
                     /translation="DGLNKLCADTTIPANCNAIVLDGSSYGGSQNYWVIEHIRSNGYN
                     GGAGSKGNAIGFISEDAGYSAPGILVDSNTGEYNRTGLANTSAYSVAMIENLGDTTFP
                     VDECTWGNCGVRVMGNAHFARGDTNVFSMCPVGGEHGTRGYTNWNAWVISAIKTIIHI
                     EGGIPTFTGYTAPTLTVTNACVTSTLPGLSVSGTQLMANGKVIRLRGVNMGDPFWARG
                     WYGSMYSTSNYSTLAQDWHANVVRISIFPTQWKNMDRATLLAGLDTEIQAALSNGMYV
                     IISYHVIGWPDGWYKEAYPGNPADTYDSSMSVATSFWTKMAQTYGGDTRIMFDLWNEP
                     VHPDDFTLYGSTPNPLWAPLKSMYESLIQAVRNNGGQNIVIATGNRWASWLVGIKDNP
                     LADSKVIYAYHKYSVTGYNTASVWNNDLGGLLGVKPIIITEWGYEDSDVTNPSWPGTQ
                     ASYGDPFTQWLDSNNLSNLAWMYHHDWTPALIKSDSTTTLYGTFVKGYFTNLSIGTTK
                     PDLIVTNVTVNPTMPGNNQTFTVNITVKNQGASTGANTIYRDVYIDRDPSTLLDPITG
                     CPPPGDFYTSDAYASIPAGMTDTKSVAVTGGLPTGNHQIWVYVDARCLIDEGANSNNT
                     P"
     gene            2031..>4084
                     /locus_tag="PLG52_06960"
     CDS             2031..>4084
                     /locus_tag="PLG52_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB41222.1"
                     /translation="MNTPVASPKEMVRILVVDDHPNTATTLARALAQLGPTVDVVSAV
                     SGTDALEKVKDKGVDILFTDMIMPEMTGLELIEKMQNHPGGKPAYTYLVTAYDVPGLK
                     VTAQRLKVNDVIIKPVRPEKICQIATAAMDEMKHAANPAGKKSATKRKFKILVADDVM
                     DNVTLLTRYLEYEGYDQVVARDGLEALNKIRDEVPDLILLDVNMPNKDGFTVLSEVRA
                     DSAIAHIPVIILTAARLDPSDVQSGLNLGADDYVTKPFDRHELMARIRTKLRVKEAED
                     ITRRRNRELNLLPEIGKQLSARLDPKDLANVLLKRTAETLGAIQANMYILDSEEKEIQ
                     EKYEVVLNPSGAAENLTPPAKLIQHVQENHQGVVIEDAVNDPLWQVDAQTHIRSALVV
                     PIFGRYALLGLIILTHEQESYFSSDHLLLLQAIASQAAIAIENTRLYSVVLQEQKKVA
                     AILRHASEAILLFDEQGNLTLLNPAGEQLFLMHPMKMNQPLPTDYGYDGLIKLIEDAR
                     TSQMSISGEIVWVDQRTFTAFVAPIEDGGTVAILHDVSRFKDLDQVKNEFIATASHDL
                     KNPLTSIGGFAQLLLQAGPLNENQKEFVDRIQHSVKSMSELIQNMMALAQADLNATQK
                     HEPVDIKEVLDEVVNEFAPQARGKEQNLEFTSPTSHVQVNGDSLHLRQAFRNLLGNAI
                     KYT"
BASE COUNT         1105 a          987 c          958 g         1034 t
ORIGIN      
        1 tggacgggtt aaataaactt tgtgcagata caactattcc tgccaattgc aatgcgattg
       61 tgttggatgg ttcctcatat ggcggctcac agaattattg ggtgatcgaa catattcgaa
      121 gcaacggata taacggtggg gcgggaagta aaggcaatgc aattggtttt atctcagaag
      181 atgccgggta tagcgctcct ggcatactcg tggattcaaa cacaggtgaa tacaaccgta
      241 ccgggttggc gaatacatca gcatatagcg tagcaatgat tgagaattta ggagatacca
      301 cattcccggt cgatgaatgt acatggggca attgcggcgt gcgcgtaatg ggcaatgcgc
      361 attttgcacg tggtgatacg aatgttttca gcatgtgccc agttggaggg gaacatggca
      421 cacgcggtta taccaattgg aacgcatggg tgatctccgc catcaagacc attatccata
      481 ttgaaggtgg aattcccacg ttcaccggat acactgcacc gactttgaca gttacgaatg
      541 cttgtgtaac atccactcta ccggggcttt cagtttctgg cacacaactt atggcaaatg
      601 gaaaagtcat ccgtttacgc ggcgtcaaca tgggtgaccc attctgggcg cgcggctggt
      661 atgggtctat gtattcgacg tcaaattatt cgacgcttgc tcaagattgg catgccaacg
      721 ttgttcgtat tagtatcttc cctactcaat ggaaaaacat ggatcgtgcc actttattgg
      781 cagggttaga tacagaaatt caagctgcac ttagcaacgg catgtatgtc atcatttctt
      841 accatgtcat tggttggccc gatggctggt acaaagaagc ctatccaggc aaccctgcag
      901 atacttacga ctccagcatg agtgtggcaa cttcattctg gacaaaaatg gcacagacat
      961 atggcggcga tactcgcatc atgtttgact tatggaatga acctgtccac ccagatgatt
     1021 ttacgctgta tggttccaca ccaaatccgt tatgggcgcc cttgaagagc atgtatgaaa
     1081 gtcttattca agcggtaaga aataatggcg gacagaacat tgtcattgcc acgggcaatc
     1141 gttgggcaag ttggttagtg ggtatcaaag ataatcccct cgctgactcc aaagtgatct
     1201 acgcctatca taaatattca gttacaggct ataacactgc cagcgtttgg aataatgact
     1261 taggcgggtt acttggcgta aagccgatca tcatcaccga gtggggctac gaagacagtg
     1321 acgtgaccaa cccatcctgg cccggcacgc aagcctcgta tggcgatcca ttcacgcaat
     1381 ggctggacag taacaatctc agtaatctag catggatgta tcatcacgac tggacgcccg
     1441 cactaatcaa atcagatagc acaactacgc tttatggaac gtttgtaaaa ggttatttca
     1501 ccaacctttc cattggcact accaaacctg atctcatcgt caccaatgtc accgtcaacc
     1561 caaccatgcc gggaaataat cagaccttta ccgtaaatat tactgtaaag aatcaaggcg
     1621 cttccaccgg agccaacaca atctaccggg atgtatacat cgaccgcgac ccttctaccc
     1681 tgcttgaccc catcaccggc tgccctccgc ccggcgattt ctacacctcc gatgcttatg
     1741 ccagtattcc cgctggcatg accgacacca agtcagtcgc agtcaccggc gggcttccaa
     1801 ctggcaacca ccaaatttgg gtatatgtag atgcgcgttg cctgatcgat gagggagcaa
     1861 acagcaacaa tactccatag ttcggatttt tacagcgagg taatcgccta atggacaaaa
     1921 acccgcctcc ggtccacgcc tgaggcgggt ttttcgttcc caacttttcc caacttttat
     1981 cctatccaac ctaactaccc ttgcggagaa tccggcgaga atatatggcg atgaataccc
     2041 cagtcgcttc tcccaaagaa atggtgcgca ttctggttgt ggatgaccac ccaaatacag
     2101 caacaactct tgcgcgcgcg ctcgcccagc ttggacccac cgtagatgtg gtttcggctg
     2161 tgagcggtac cgatgcgctt gaaaaggtca aagataaagg tgtggatatt ctctttacag
     2221 acatgatcat gcctgaaatg accgggctgg aattgatcga aaaaatgcaa aatcaccccg
     2281 gtggcaagcc tgcgtatacc tatctggtca ctgcgtatga tgtgccgggt cttaaggtta
     2341 cggcgcaacg cttgaaggtt aatgacgtca tcattaagcc tgtgcgccca gagaaaattt
     2401 gccagatcgc taccgctgcg atggatgaaa tgaaacatgc cgcaaaccct gcggggaaga
     2461 aatcggcgac caaacgcaaa tttaaaattc tcgtagcaga tgatgttatg gataacgtca
     2521 ctctgttaac ccgttatctt gaatatgagg ggtatgacca ggttgttgct cgcgatgggt
     2581 tggaagcctt gaacaagatt cgtgatgagg tgccagatct cattcttttg gatgttaaca
     2641 tgccgaacaa agacggcttc accgtgcttt cagaagtccg cgcagattcg gcaattgctc
     2701 atattccggt cattattttg accgccgctc gtttagatcc ctctgatgtg caatctggct
     2761 tgaaccttgg tgccgatgat tacgttacca agcctttcga ccgtcatgag ctaatggcgc
     2821 gcattcgcac caagttacgt gtgaaagaag ccgaagatat tacgcgtcgc cgcaaccgcg
     2881 agttaaattt attgcctgaa attggcaaac aactcagcgc tcgtttagac cccaaagacc
     2941 ttgcaaatgt tttgctcaag cgcaccgccg aaactttggg tgctattcaa gccaatatgt
     3001 atattttaga ctctgaagaa aaagagattc aagaaaagta tgaagtggtt ttgaatcctt
     3061 caggggctgc tgaaaacctt actccgcctg ccaaacttat tcaacacgta caggaaaatc
     3121 atcaaggggt ggtaatcgaa gatgcggtca atgatccgct ctggcaagtg gatgctcaaa
     3181 cccacatccg ttcggctttg gttgtgccca tctttggtcg ttatgcatta ttggggttga
     3241 tcattctgac tcacgaacag gaaagttatt tttcaagcga ccatttgttg ttattacaag
     3301 ccattgcaag ccaggctgcc attgccattg aaaacacgag attgtattcc gtggtattgc
     3361 aggagcagaa aaaagttgct gcgatcttaa gacacgcctc agaagcgatt ttgctcttcg
     3421 atgagcaagg caacctaacc ttgctaaacc ctgcgggtga acagcttttt ctgatgcacc
     3481 ccatgaaaat gaatcagcct ttgcctacgg attatggata cgacgggctt attaagttaa
     3541 ttgaagatgc acgtacatct caaatgtcta tttcgggcga aatcgtttgg gttgaccaac
     3601 gcacattcac tgcctttgtc gctccaattg aagatggcgg gaccgttgcc atcttgcacg
     3661 atgtgagccg cttcaaagac ttggatcagg tcaagaatga attcatcgcc accgcctcac
     3721 atgacttgaa gaatccgctt acttcgattg gtgggtttgc gcaattgctg cttcaggcgg
     3781 gtccacttaa cgaaaaccaa aaagagtttg tagaccgcat tcaacactcc gttaagagca
     3841 tgagcgaatt aatccaaaat atgatggcat tggctcaggc agatttgaac gctacccaaa
     3901 agcacgaacc tgttgacatt aaagaagttt tggatgaagt ggttaatgag ttcgctccac
     3961 aagcgcgcgg caaagaacaa aatcttgaat tcacctcacc cacctctcat gtgcaggtaa
     4021 atggtgactc gctccattta cgccaagcat tccggaattt actgggaaac gccattaagt
     4081 acac
//
LOCUS       DAOQNJ010000200         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14094_length_4076_cov_8.920915, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000200 DAOQNJ010000000
VERSION     DAOQNJ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14094_length_4076_cov_8.920915"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLG52_06965"
     CDS             complement(<1..724)
                     /locus_tag="PLG52_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41223.1"
                     /translation="MDDYLVNATGLKKSYGANHAVNGVSLHIPPGEIYGLVGADGAGK
                     TTTMRLLVGALLPDAGEISVCGYDVLKQTEQARSTIGYLSQRFSMYEDLTVLENIRFF
                     AEIRGLSSTEWLPRSLEILDFVGLEKFKDRRAGQLSGGMKQKLGLASALVTRPRLLLL
                     DEPTTGVDPVTRQDFWQLVIKLVGSREGDGVSVIISTPYMDEASRCHRVGFMKNGRII
                     TEDTPSNLRSLLNGRILELRGSP"
     gene            complement(791..2101)
                     /locus_tag="PLG52_06970"
     CDS             complement(791..2101)
                     /locus_tag="PLG52_06970"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB41224.1"
                     /translation="MQNKLPPLPVRIVIAVIVLGTLAYFGFQSLKDDTNGSLTASGTI
                     EATIVNVAPETAGKVTDVRADEGESVTKGQSLLSLDASLLTAQQAVASAAVDSATAAL
                     AAAQTKYDQTLEAAFAAQAAQRSKDWQASAPSEFDQPNWYIEQDQQVSSAQAELDAAK
                     AEIDAANENLDRVITDLKNSEFLKTEQELADARAAFFIAQQVKDQASSASDNSGLTDA
                     AQDYYDDAVTRLNDAEEAYNDLMNSDEADDVRYARGQVLVAQQRYDAAYARLLTLQTG
                     ENSPAVIAAGKELDKAKSALAQAEASLALVQAQIAKLEVHAPMDGVILTRNVEPGEFV
                     QPGAAALTIGDLSDLTITVYVPENRYGEISLGQTAEVTVDSFPGVTFTATVIQIANKA
                     EFTPRNVQTVEGRSATMFAIKLSVQDAEGRLKPGMPADVIFISK"
     gene            complement(2143..3273)
                     /locus_tag="PLG52_06975"
     CDS             complement(2143..3273)
                     /locus_tag="PLG52_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41225.1"
                     /translation="MFNTRIVSIVRKEFVQIFRDPRSLALIIVMPIIQLFLLGYAATT
                     DVKNVPIAVLDQSQSAQSRELLDAFRAADYFRIDYVVGSTEEYQLLIESGKIRAALVI
                     PPDYDVQVLDGKAQVLFVLDGSDGSVGATALSTARLIGQSYSTKVQTSQFALRGSGSL
                     PTQPVEVRTQVWYNPDFQSAYFMIPGVIGMILSFITTILTATTIVRERERGTIEQLIV
                     TPIRSWELIVGKLLPYVILAFVETFEILIVGHLAFDVPVRGSLLLITLASGLFLMSSL
                     GIGLFASTIANTQQEAMLSVMMFNLPMIFLSGFFFPVQAMPQFLQFVSLAIPLRYYLV
                     VIRALMLKGVGFAAIQSEIISLTIFGIVIMGIAALRFRKRLD"
     gene            complement(3278..3886)
                     /locus_tag="PLG52_06980"
     CDS             complement(3278..3886)
                     /locus_tag="PLG52_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB41226.1"
                     /translation="MAEETHTKGERTRIQIEDAAIELFIEQGYHATSMRQIADRADLA
                     LGGIYNHFKSKDEIFEAIIVDKHPYKRLLPLILEAEGHTMEDFLGHAARVVIKELTSQ
                     PYYIKLMLIEIAEFNGVHGAVLIKEIGPKLLPVFERIIKTRKNLRLTNPAMLMRTFIG
                     MVVSYMITEMLISNSMLNKMMPKNPIDAYVDIYLHGIVKSEA"
BASE COUNT         1106 a          997 c         1002 g          971 t
ORIGIN      
        1 aaggtgagcc gcgcaattca aggatacgtc cgtttagaag tgaacgcaaa ttagatgggg
       61 tatcttctgt aataattctg ccatttttca taaagccaac acgatgacaa cgcgaagctt
      121 cgtccatgta tggagtggag atgatgaccg aaacgccatc accttctcgt gaaccaacca
      181 gcttgatcac caattgccaa aaatcctgac gagtcacagg atccacgccg gtggtgggtt
      241 catcgagcaa gagcaaacgc ggacgagtga ccaatgccga ggcaagccca agcttttgct
      301 tcatgccgcc agaaagttgc ccggcgcggc gatctttgaa tttttccaaa ccgacaaagt
      361 ccagaatctc caacgaacgc ggcagccatt cggtggaaga aagccctcgg atctcagcaa
      421 agaaacggat attctccaac acggtcaggt cttcatacat cgagaagcgc tgcgaaagat
      481 aaccaatggt tgagcgggct tgttcggttt gcttcaacac gtcgtatccg caaaccgaaa
      541 tttctccggc atctggcagc aacgccccaa ccagtaagcg cattgtggtc gtcttacctg
      601 ccccatctgc accgacgagt ccataaattt cgccgggagg gatatgcaaa gaaacaccat
      661 tcactgcatg attagcgccg tacgattttt ttaatccagt tgcattaact agataatcat
      721 ccataatttc taccttcaac tggtggtttc gatacgctcc ataaaaaacg cggagcgact
      781 caaccaccga ttatttacta ataaagataa catccgcagg cataccgggt ttcaaacgtc
      841 cttcggcatc ttgcacactg agtttgatcg caaacatggt ggcgctgcgt ccttccacag
      901 tttgcacatt gcgcggagtg aactcggctt tgttcgcaat ttggatgacg gtggcagtga
      961 aagtcacgcc ggggaaagag tccacagtca cttcggcggt ctgcccaagg gagatctcgc
     1021 cgtagcgatt ctctgggacg tacactgtga tggtcaggtc tgagagatcg ccgatggtaa
     1081 gagctgccgc acccggctgg acaaattctc ccggctcgac attacgggtg agaatgactc
     1141 cgtccattgg ggcgtggact tcgagtttag caatctgtgc ttggaccaat gccaggctgg
     1201 cttctgcttg tgccagtgca gactttgcct tatccaattc ttttccagcc gcgatcacgg
     1261 caggagaatt ttcgccggtc tgtaaggtga gcaagcgggc ataggcggca tcataacgct
     1321 gttgcgccac gaggacttgt ccgcgggcat agcgcacgtc atcggcttcg tcgctgttca
     1381 tcaagtcgtt ataggcttct tcagcgtcat tgaggcgggt aacagcatcg tcgtaataat
     1441 cttgggcggc atcggttaat ccgctattat ctgaagcgct ggacgcctga tctttcactt
     1501 gttgtgcaat gaaaaatgca gcacgagcat cggcaagttc ttgttctgtt tttagaaact
     1561 cagaattctt caggtctgta atgacacggt ccaagttttc gttggctgca tcaatttcag
     1621 ctttggcggc atcaagttct gcttgagccg atgaaacctg ctggtcttgt tcgatgtacc
     1681 aattgggttg gtcaaactca cttggcgcag aagcctgcca atctttggag cgttgtgcag
     1741 cttgggcggc aaaagcggct tcgagcgttt ggtcatattt ggtttgggca gcggcaaggg
     1801 cggcggtggc agaatctact gcggcggagg caacggcttg ttgggcagtt aacaggcttg
     1861 cgtcgaggga gagaagcgat tgtcccttcg tcactgattc gccttcatcg gcgcggacat
     1921 ccgtcacttt gccagcggtt tcaggggcga cgttcacaat ggtcgcttcg atggtgccag
     1981 aagcggtcag actgccattg gtatcatctt tgagcgattg aaagccgaag tatgccagcg
     2041 tgccgagcac aatcacagcg ataacgatgc ggacgggcag agggggtagt ttattttgca
     2101 tgagagactc cagaattgaa agttaaaagt acaagttgaa aattagtcga ggcgttttct
     2161 aaagcgcaag gcagcaatgc ccataataac gatgccaaaa atggtaaggg aaatgatctc
     2221 gctttgaata gcagcaaagc ctacgccctt aagcatgagt gcgcggatca ccacaaggta
     2281 atagcgcaat ggaatcgcca gagatacgaa ctgcaaaaac tgcggcatgg cttgcactgg
     2341 gaagaaaaag ccagagagaa agatcatcgg caggttgaac atcatgaccg acagcatggc
     2401 ttcttgctgg gtgttagcaa tcgtagaagc gaacaagcca atgcctaaac tggacatgag
     2461 aaacaagccc gaagccagcg taatgagcag taagcttccg cgcacgggca catcaaacgc
     2521 aagatgcccg acgataagaa tttcaaaggt ctccacaaaa gctaaaatga catagggcaa
     2581 caacttcccc acgatcaact cccacgagcg gattggcgta acaatgagct gttcgatcgt
     2641 gccgcgctcg cgttcacgga cgatggtagt agctgtgaga atagtggtaa tgaaagaaag
     2701 gatcatcccg atcacgccag ggatcatgaa gtatgccgat tgaaaatcgg ggttgtacca
     2761 aacttgagtg cgtacttcca caggttgtgt tggtaatgaa cccgaaccac gcagtgcgaa
     2821 ttggctcgtt tgcaccttgg ttgagtaaga ctgaccaatc aaccgcgcag tggaaagtgc
     2881 cgttgcgcct acactgccat cggaaccatc taacacaaaa aggacttgtg ctttgccatc
     2941 taaaacctga acatcataat caggcggaat gaccaacgca gcacggatct tgcccgactc
     3001 aatcagcaat tgatactctt ccgtagaacc caccacataa tcaatgcgga agtaatcggc
     3061 ggcgcgaaaa gcatctaaca attcacgtga ttgagcgctt tggctttggt caagcacagc
     3121 gataggcacg ttcttcacat ccgtggtggc ggcatacccc agcaaaaaca gctgaataat
     3181 gggcataacg atgatcagcg ccagtgaacg cgggtcgcgg aatatctgca caaattcttt
     3241 acgaacaatc gaaacgatac gggtattgaa catggaatca agcctcagat ttaacaatgc
     3301 catgcaggta aatatcaaca taggcatcga tgggattttt aggcatcatt ttatttagca
     3361 tggaattcga tatgagcatt tctgtgatca tgtacgaaac caccataccg ataaaagttc
     3421 gcatcagcat ggctgggtta gtcaggcgca gattcttgcg agtctttatt attcgttcaa
     3481 agaccggcaa caacttggga ccgatctcct tgatcaacac agcaccatgc acgccgttaa
     3541 actcggcgat ctcaatcagc atcaacttga tgtaatacgg ctgagacgtc agttccttga
     3601 taaccacccg cgccgcatga ccgagaaaat cctccatcgt atgcccttct gcttccaaaa
     3661 taagcggtaa aagcctttta tatggatgtt tatcaacaat tatggcttcg aatatctcgt
     3721 ctttactctt gaagtggttg taaataccgc ctaatgccaa atctgcccga tcggcaattt
     3781 gacgcatcga tgtagcatgg tagccctgct caatgaacaa ctcaattgca gcatcttcaa
     3841 tttgaatacg agtgcgctcg cctttggtat gtgtttcttc tgccatgacg tctccagaaa
     3901 ataaatgaac gaacgttcgt ttttatttta gaccaagtag gtttactttg tcaaggagga
     3961 aaaataagtc catacacctc caccccacaa tgtatcaaca acaccataag gtaaaatcac
     4021 tttatgctct ccccacttga acaagctatt cgcaaagacc ttaaacacaa tgttac
//
LOCUS       DAOQNJ010000201         4062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14173_length_4062_cov_8.099326, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000201 DAOQNJ010000000
VERSION     DAOQNJ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14173_length_4062_cov_8.099326"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLG52_06985"
     CDS             <1..863
                     /locus_tag="PLG52_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41227.1"
                     /translation="REKEMSDVIDSKIRADFSRARFKSFINQVFSVLSGQRNTLLSYD
                     DIKEKLRIGGPIYRGMKTVRVEQIAGSLNRYHEFDRAFLPKEDQLASRWQKVDRAFYE
                     DIHLPPVVLYKVGQVYFVVDGHHRVSVAREQGQEYIEAEVRECATRVNITPDIRPEDL
                     EVLGAKVDFLERTGLDKYRPDANIKLTIPDGFTRMLEHIAVHRYFMGLDFKRDISEQE
                     AVEHWYDKVYVPIVSIIRESGLLDEFPDKTESDLYLWALDHQHYLYQEEGQPLQPPEL
                     AAKQFILENE"
     gene            936..>1780
                     /locus_tag="PLG52_06990"
     CDS             936..>1780
                     /locus_tag="PLG52_06990"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNB41228.1"
                     /translation="MTPLHPLAGVYAAAVTPYLGAPKTAGKPDTAFDFESLAMFLHFL
                     AGRGCHGALLFGTTGEGPSFSPREREALLRSIRVIKHQVRDFKFLVGTGTPSLTETIE
                     LTKLAYDLGYDGTVVLPPYYFRKANDEGLFRWFSELIENAVPQGKHLLGYHIPVMTGV
                     GFSLDLLERLKTKYPNQFAGIKDSSHDEAFATAVGQRFGKELLVLNGTDSYFHHALKN
                     NAQGAITAPANLISDNLREVWDLFQAGKDPSEVQAKVIEQRHFLEGYSPIAPVLKGLL
                     HKVHGL"
     assembly_gap    1781..1880
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1881..2489
                     /locus_tag="PLG52_06995"
     CDS             <1881..2489
                     /locus_tag="PLG52_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41229.1"
                     /translation="VVRRVTGGRAILHTDELTYSVTGSADEPVLAGGVLESYNRLAQA
                     LLHAVRELSVPVEMKEHEDGHTQQNLNPVCFEVPSTYEITVDGKKLIGSAQARKKEGV
                     LQHGSLPLTGDLTRICDALIFENESARQNAKERLLARATTIESVLGVGINWERAAQAF
                     VRGFEAELGIQFERGEMSTSEIQRTEELVKEKYAHVSWTERI"
     gene            2486..3538
                     /locus_tag="PLG52_07000"
     CDS             2486..3538
                     /locus_tag="PLG52_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNB41230.1"
                     /translation="MKKILVYGSYGYTGKLIVEHAMQEGLQLILAGRDEKQLREQAEK
                     YKLEYRVFAVEETAKLDAALQEVDAVLHCAGPFVLTYRAMVEACIRNKKHYVDISGEI
                     EGFEALALMNDEAKRAGVMLLPGGGFDVVPSDCLIAYTASKLPNASDLKLYIKSIGSG
                     ISRGTARSGIENSHRQGRIRRDGKIVSVPNLYDSKDIDFGRGPSKLVTMGWGDVSTAY
                     HSTGIPNVTVYMSFPKSMISVMKFTKVAGPLLYSRAARNFIKWLIGIFLAPGPSREKN
                     KTGFSLLIAEVTDGTKTVRAKLRTPEAYYLTALTSVEIMKRILSNELKPGFQTPSKMY
                     GADFILQFNGVEREDL"
     gene            3678..>4062
                     /locus_tag="PLG52_07005"
     CDS             3678..>4062
                     /locus_tag="PLG52_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNB41231.1"
                     /translation="MKFETLAIHAGQEPDPNNGAVMTPVYLTSTYKQDGIGKPRQGYE
                     YSRTLNPTRKALQDCLAELESGNWGLAFASGMAATDTVLRMLGQGDHVVAGNDVYGGT
                     FRLFDKILRRFGLDFTFADTTDPENL"
BASE COUNT         1051 a          929 c         1018 g          964 t
ORIGIN      
        1 aaagagagaa agaaatgtca gacgtaatag attcaaaaat acgtgcagat tttagtcggg
       61 cacgtttcaa atccttcatc aatcaggtat tctctgtctt aagcgggcag cgaaatactt
      121 tgctttctta tgatgatata aaggaaaaac tccgtattgg gggacccatc tatcggggta
      181 tgaaaactgt ccgcgtggaa cagattgcgg ggagtctgaa ccggtaccat gaattcgacc
      241 gagccttctt acccaaggaa gaccaacttg ccagccgctg gcaaaaagtc gatcgtgctt
      301 tttatgaaga tattcactta ccgcctgtgg ttctatataa agtgggtcag gtgtacttcg
      361 ttgtagatgg acatcatcgt gtgtcggttg ctcgtgagca aggacaggaa tatatcgaag
      421 ctgaagtgcg agaatgtgca actcgggtca acatcacgcc agatattcgt cctgaagacc
      481 ttgaagttct cggtgccaaa gtggattttc ttgagcgtac cggtttggac aaataccgac
      541 ccgatgcaaa tatcaaactg acgatccctg atgggttcac acgtatgctt gaacatatcg
      601 ccgtccatcg ttattttatg gggcttgatt tcaaacgcga tatttcagaa caagaagctg
      661 tggaacattg gtacgacaag gtgtacgtcc ctatcgtatc cattattcgt gaaagcggac
      721 tcttggatga atttccagat aagactgaaa gcgacctata tctctgggcg ttagaccatc
      781 agcattatct atatcaggaa gaagggcaac cacttcaacc gccggaatta gcagctaaac
      841 aatttattct cgagaacgag taaactagta gggacgaccc caaagaaaga ttttgaaagg
      901 tcgtccctac tttattttgg agacaaagtt catctatgac accccttcat ccattagcag
      961 gtgtttatgc tgctgcagtt accccctacc tcggtgcacc taaaacagcc gggaaaccag
     1021 acacggcttt tgactttgag tcactggcga tgtttcttca cttcctcgcc gggcgcggat
     1081 gtcacggcgc gttgctattc ggaaccacag gcgaaggtcc gtcgttctcg ccgcgcgaac
     1141 gtgaagctct gcttcgttcc attcgcgtca tcaaacatca ggtgcgtgac tttaagtttt
     1201 tggtcggcac cggaacgccc agcctgaccg aaaccatcga actgaccaaa cttgcttatg
     1261 accttggtta tgacggaact gtggttcttc cgccgtatta cttccgcaaa gccaatgatg
     1321 agggattgtt ccgttggttc agcgaattga tcgaaaatgc tgtaccgcaa gggaaacacc
     1381 tgctcggcta tcatatccct gtgatgacag gcgttggctt ttcgcttgac ctgctggaac
     1441 ggctcaaaac gaaatatccg aaccaatttg caggtatcaa agattcatcg catgatgaag
     1501 cctttgcgac ggcagtgggt caacgttttg gaaaagaact gctggtcttg aatgggactg
     1561 actcttactt tcaccacgcg ttgaagaaca atgctcaagg cgcgatcacc gctccagcca
     1621 acctcatctc tgataatctg cgcgaggtct gggacttgtt ccaagcgggc aaagacccca
     1681 gcgaggtgca agccaaagtt atagagcaac gacattttct tgaaggttac tctccaattg
     1741 caccggtctt gaaaggcttg ctccataaag tacatgggct nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn gtcgtccgcc gcgtgaccgg gggacgggcg attctgcata
     1921 ctgacgaatt gacctactct gtcaccggct cagcagatga acctgttctc gcaggcggtg
     1981 tacttgaatc ttataaccgc ttggcgcagg cattactcca tgccgtacgc gaattgagcg
     2041 tgcctgttga aatgaaagaa catgaagatg ggcatactca gcagaacttg aacccggtct
     2101 gtttcgaagt tccatccacc tatgagatca ccgtcgatgg gaaaaagttg atcggctcgg
     2161 cacaggcgcg gaagaaggaa ggtgttctgc aacatggctc ccttccgttg accggtgacc
     2221 tgactcgaat ttgcgatgcg ctgatttttg aaaatgaatc tgcgagacaa aacgcgaagg
     2281 aaagactcct cgctcgggcg actacaatcg aatccgttct cggcgtagga atcaattggg
     2341 aacgggctgc ccaggctttc gtccgcgggt ttgaggcgga gttgggaatc caattcgagc
     2401 gcggagaaat gtctacatcc gaaattcaac gcacggaaga acttgtcaaa gaaaaatatg
     2461 cgcatgtttc atggacggaa cgaatatgaa aaaaattctg gtgtatggct cgtatggata
     2521 tacagggaaa ttgatcgttg aacatgccat gcaagaaggt ttgcagttga tcctcgccgg
     2581 gcgcgatgaa aaacagcttc gtgagcaggc agaaaaatac aagttggaat atcgcgtctt
     2641 tgcagtggaa gagactgcca agctcgatgc cgccttgcaa gaagtggatg cggtgttgca
     2701 ttgtgcaggt ccgttcgtgt tgacgtatcg agccatggtc gaggcatgca tccgcaataa
     2761 aaaacattat gtggatatca gcggcgagat cgaaggcttt gaggcgctgg ctttgatgaa
     2821 tgatgaagcc aaacgcgcag gtgtgatgtt gctgcccggt ggcgggtttg atgtggtgcc
     2881 atccgattgt ttgatcgcct acacggcaag caaactgcca aatgcatcag acctgaagtt
     2941 gtatatcaaa agcataggca gtggaatctc tcgcggcacg gcacgttctg gcattgagaa
     3001 cagtcatagg cagggacgca ttcgccgcga tggaaagatc gtcagcgtgc cgaatttata
     3061 cgatagcaaa gatattgatt ttgggcgcgg accttcgaaa cttgtgacaa tgggctgggg
     3121 cgatgtcagc acggcgtatc acagcacggg tattccgaat gtgactgtgt acatgagctt
     3181 tccaaaatcc atgatctcgg tgatgaaatt taccaaggtt gcaggtccgc tgctttactc
     3241 acgagcggca agaaacttca tcaaatggtt gatcggaata ttccttgcgc cgggtccttc
     3301 acgcgaaaag aacaaaaccg gtttctcatt gctgatcgct gaagtgaccg acggaacaaa
     3361 aactgtccgc gcaaagttga gaacaccaga agcgtattac ctgacagcac tgacttcggt
     3421 tgagatcatg aagcgcattc tatcaaatga actcaagccc ggctttcaga cgccaagcaa
     3481 aatgtatggt gcagatttta ttttgcaatt caatggggtt gagcgcgaag acttgtaggt
     3541 tcaacaaaat gtcaccctga gcgacatcga agggtttcta ctacaatgta agagactctt
     3601 cggtcgcaca gaacgctccc tcagagtgac atgaattttc tcttttataa tcacaaaaac
     3661 cgaccaaagg agcatgaatg aaatttgaaa ccctcgccat tcacgcaggg caggaacccg
     3721 acccgaacaa cggcgccgtg atgacgcctg tgtatctcac atccacctat aaacaggacg
     3781 gcatcggcaa gccgcgtcag gggtacgaat attcacgcac attgaatccc actcgtaagg
     3841 cgttacaaga ttgtcttgct gaattggaaa gtggaaactg gggacttgct tttgcctcag
     3901 gcatggcagc caccgatacc gtcttgcgta tgttaggaca aggtgaccat gtcgtcgcgg
     3961 gcaatgatgt gtacggcggt acgttccgcc tgtttgacaa gatcctgcgc cgcttcggtc
     4021 tggactttac atttgctgac acgaccgatc ctgagaacct cg
//
LOCUS       DAOQNJ010000202         4057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14211_length_4057_cov_10.453273, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000202 DAOQNJ010000000
VERSION     DAOQNJ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14211_length_4057_cov_10.453273"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1226
                     /locus_tag="PLG52_07010"
     CDS             138..1226
                     /locus_tag="PLG52_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNB41232.1"
                     /translation="MANKKKVRVAIIGVGNCASSLVQGVQFYKNAKNDDVIPGIMHPQ
                     LGEYHIGDIEFTLAIDINATKVGKDLSEAIFAEPNNTYKFCDVPHLNVPVVRGMTHDG
                     LGKYLSQVIEKAPGSTADIVRLLKETKTEVVISFLPVGSEMATKWYVEQIIEAGCAFV
                     NGIPVFIASSEYWGKRFADAGLPILGDDIKSQVGATILHRQLATLFVDRGVKIERTYQ
                     LNFGGNTDFLNMLERERLESKKISKTNAVTSMIPYEINPDNVHVGPSDHVPWLTDRKW
                     AYIRMEGTTFGNVPLNMEVKLEVWDSPNSAGVMIDALRCAKIALDRELAGPIVGPSSY
                     FFKTPPKQFRDEIAKQKVEDFVSGKSDE"
     gene            1307..2485
                     /locus_tag="PLG52_07015"
     CDS             1307..2485
                     /locus_tag="PLG52_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41233.1"
                     /translation="MRRKTTRHSLIVSRLFLSLSLFATLLAACQPAAPTTDINAALTA
                     AFETAVAQINAPTATPQITDTPIPTATVPRTPPALPGTYQSGILKPNDIPRTYIADSC
                     QYLKDKWDSTKSAPGTVVMTIMSHRINQDAATSDPASLTISSKDLKRLMNSLHDAGFT
                     AINMQQMSDFMYNNAKIPQLSVLIIVDDRSSSSKYNDHFRKYYEEWGWPVVNAYIGLD
                     ERPDLWAENAVLSSEGWVDYEAHGYIHNIPISDGSTDDFITSEMGGVITTFQKYFNKT
                     PIAYIWPGGGFSKRAAEIGVQLGYKLGFTVNPRGPVMYNWIPQADAYNEDNPYAIPET
                     PAGNPLMTLPRYMDITAGDHIDEVRVMSQEAAAYAEQNKATELEYYNIVCAPTYGPIP
                     "
     gene            2589..3686
                     /locus_tag="PLG52_07020"
     CDS             2589..3686
                     /locus_tag="PLG52_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41234.1"
                     /translation="MQSPRNTFIPIFLRLTSIVSLVSILVSCQLSPSITNTAEAPTEI
                     ADQTIPVLPETFQTDLLNPVDVPHTYVEETCRYLRNKWNPVNAQPGTVVMLILIKDIN
                     RGAVELPNSIRITDFYDLLEQLKAQGFEAINTEKLQAFLERNLPIPPRSVVLIQDGNH
                     TEEYFDKNYRWYYEQWGWPVVNGFVSDPNIDPILREGNFSLEYEGFIDHQARGITPDV
                     VLTDDIAKTIIARELQGSLEGFAEQLGKTPNAIIWPNGGFGIRPIEAARQLRFKLGVT
                     QNPRGPVMYNWVPLANSVDDARPTLIPEGKVGDPLMTLPAYSPQEAFNAIDSVRNIGN
                     AAQAYAESNKDAEYQYYDIVCSDKYGPIPTP"
     gene            3700..4041
                     /locus_tag="PLG52_07025"
     CDS             3700..4041
                     /locus_tag="PLG52_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNB41235.1"
                     /translation="MSDCIFCKIIKGEIPSTNVYKDEFVTAFRDINPAAPTHILIVPN
                     KHIDSVNMLIPDDEPLIGKMFSIAKQLANKEGIAESGYRLLVNTGEEAGQTVFHIHLH
                     LLGGRTMRALG"
BASE COUNT         1180 a         1074 c          821 g          982 t
ORIGIN      
        1 aggagacgtg aaaggatttt cgtctgtgag tccgtcatac aaataacctt gggtaagatt
       61 atacttcacg gttgtatctg aaaatcgacc ctcataattc aataaggaaa ctgtaatcca
      121 cctcaggagc taaacaaatg gcaaataaga aaaaagtacg tgtggcgatc attggcgtag
      181 gaaactgtgc ctcttccctc gtgcaaggcg ttcaattcta taaaaatgcc aaaaatgacg
      241 atgtgattcc cggcattatg cacccgcaat tgggtgaata tcacattggt gacattgaat
      301 tcaccctggc gatcgacatc aatgccacca aggttggcaa agatttgtct gaagcaattt
      361 ttgcggagcc gaacaatacc tacaagttct gtgacgtgcc tcacctcaat gtacccgtcg
      421 tgcgcggcat gacccatgat gggttgggca agtatctcag ccaggtcatt gagaaagcgc
      481 ccggctccac tgccgatatt gtacgcctat taaaagaaac aaagaccgaa gttgtcatca
      541 gcttcctccc ggtaggctca gaaatggcaa ccaaatggta tgttgaacag attattgaag
      601 caggttgcgc ctttgttaac ggtattccgg tattcattgc ctcctctgaa tattggggca
      661 aacgcttcgc cgatgccggt ttgccaattc tcggtgacga tatcaaaagt caggtcggcg
      721 ctactattct tcaccgccag ctcgccaccc tgttcgtaga ccgaggcgtc aagatcgagc
      781 gcacctatca gctcaacttt ggcggcaata cagacttcct caacatgctc gaacgcgaac
      841 gccttgaatc caagaaaatt tccaagacca acgccgtcac cagcatgatc ccatacgaaa
      901 taaatccaga taatgttcac gtcggtccca gcgatcacgt cccctggtta acggatcgca
      961 aatgggcata cattcgcatg gaaggtacca ccttcggcaa cgttccgctc aacatggaag
     1021 tgaaacttga agtgtgggat agccccaact ctgccggcgt tatgatcgat gctctacgct
     1081 gtgccaagat cgcgctcgac cgcgaacttg ccggtccgat cgttggacca tcctcttact
     1141 tcttcaagac tccgccaaag caattccgcg atgaaatcgc aaaacaaaaa gtggaagatt
     1201 tcgtctctgg caaaagtgat gaataacaaa taacagaaaa ggcgcacaga aatctggtgc
     1261 gccttttctg ttattattga caatcacaaa aggatataaa ttcatcatgc gacgcaaaac
     1321 cactcgtcac tcgttaatcg tttctcgcct ctttcttagc cttagcctgt ttgccacact
     1381 tctagccgcc tgccaacccg ctgcccccac caccgacatc aacgccgcac tgacagccgc
     1441 ttttgaaacc gccgtggcac agatcaatgc accaactgca acaccccaaa ttactgatac
     1501 tcccattcca actgcaaccg ttccacgcac gccacctgct ttgccaggca catatcaatc
     1561 cggaatccta aaaccaaatg acatccctcg cacctatatc gcggactcat gccaatacct
     1621 aaaagacaaa tgggactcaa ccaaatctgc gccagggacc gtagtcatga ccattatgtc
     1681 tcaccgcatc aatcaggatg cggctacctc tgaccctgca agtttgacta tttcaagcaa
     1741 agacttaaaa cgtttgatga actccttaca tgatgctggc tttactgcca tcaatatgca
     1801 gcaaatgtca gatttcatgt acaacaacgc aaaaatccct caactttcgg tactcatcat
     1861 tgtggatgat cgttcaagtt cttcaaaata caatgaccac tttaggaaat attatgaaga
     1921 gtggggctgg cccgtggtca atgcatatat cgggttagac gaacgccccg acctttgggc
     1981 agaaaatgcc gtactctcct ctgaaggctg ggtagactat gaagcccatg gctacatcca
     2041 taacatcccc attagtgatg gttcgacaga tgacttcatc acctcagaaa tggggggtgt
     2101 gattacaacc ttccaaaaat atttcaacaa gacccccatt gcttatatct ggcccggcgg
     2161 cgggttttca aagcgagccg ctgagatcgg cgttcaactt gggtataagc tcggctttac
     2221 cgtcaaccca cgcggacccg ttatgtataa ctggattcct caagccgatg cctacaatga
     2281 agataatcca tatgccattc ctgaaacgcc agctgggaat cccttaatga ccctcccacg
     2341 ctacatggac atcactgcag gagatcatat cgacgaagtt cgtgtcatga gccaagaagc
     2401 ggctgcttac gccgagcaaa acaaagccac cgaattggaa tactacaaca tcgtctgcgc
     2461 tccaacatac ggacctatcc cctagttaat aattcttttt aagtacaacc taaacaggtc
     2521 aggcaatgcc tgacctgttt acataagcca aaactcattc tcaaaaactg gtttacaatt
     2581 ctatttatat gcaatcgcca agaaatacgt ttattcccat ctttcttcgc ctcacatcca
     2641 ttgtctcact cgtttccatt ctggtttcct gccagttaag tccatctata acaaatacag
     2701 cagaagctcc tactgaaata gcagatcaaa ccatccctgt tttaccagaa acgtttcaaa
     2761 ccgatttatt aaaccccgtt gatgttccac atacctatgt agaagagacc tgtcgttatt
     2821 tacgcaacaa gtggaaccct gtaaatgcgc aaccgggcac cgttgtcatg ctcatcctta
     2881 tcaaggatat aaatcgtggc gccgtagaac ttccaaatag tattcgcatc acagatttct
     2941 atgacttatt agaacaatta aaagcacagg ggttcgaagc gatcaataca gaaaaactac
     3001 aagcatttct ggaacggaac cttcccatcc ctccgcggtc tgttgtatta attcaagatg
     3061 ggaatcacac agaagaatat ttcgataaaa actatcgctg gtattatgaa cagtggggct
     3121 ggcccgtcgt caatgggttc gtcagtgacc ctaatataga ccctatctta cgcgaaggga
     3181 atttctcgct tgaatatgaa ggcttcatag accaccaagc gcgcggcatc acacccgatg
     3241 tagttctcac tgatgacatt gccaaaacaa ttattgcccg tgagttacaa ggctctttag
     3301 aagggtttgc agaacaatta ggcaaaacac caaatgccat tatctggccc aacggcgggt
     3361 ttggaattcg tcccatagaa gcagcgcgac agttacgctt caagctggga gtcactcaaa
     3421 acccacgcgg acctgtcatg tacaattggg taccgctcgc aaacagcgtc gacgatgcac
     3481 gaccaacctt aatccctgaa ggcaaagtcg gtgacccact catgacctta cccgcttact
     3541 caccacaaga agcattcaat gctattgaca gtgtccgtaa tatcgggaat gcggctcagg
     3601 catatgcaga atcaaacaaa gatgccgaat accaatatta tgatattgtt tgttccgata
     3661 aatatggtcc catcccgacc ccataaatag gaggctacta tgtctgattg tattttttgc
     3721 aaaatcatca aaggtgaaat tcccagcacg aatgtatata aagacgaatt cgtaaccgcc
     3781 ttccgcgata tcaaccctgc cgcaccaacc catatcttga tcgtaccgaa caaacacatt
     3841 gattccgtca acatgctcat tcctgatgac gaaccgttga tcgggaaaat gttttccatt
     3901 gccaaacaac tagcaaacaa agaaggtatt gccgaaagtg gctatcgtct tctagtcaac
     3961 acaggagaag aagccgggca gaccgtcttc cacattcacc tgcacctgct cggcggcagg
     4021 acaatgcgag ccttagggta aaatcatcga agcgatc
//
LOCUS       DAOQNJ010000203         4041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14314_length_4041_cov_9.829152, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000203 DAOQNJ010000000
VERSION     DAOQNJ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14314_length_4041_cov_9.829152"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..312)
                     /locus_tag="PLG52_07030"
     CDS             complement(70..312)
                     /locus_tag="PLG52_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41236.1"
                     /translation="MHETTSNISPPELIYLGMGLMIARILLDGTNTRGFTWLQWLQFL
                     VNGARIVMLWPLVLFIEKFEAWLKSGSSDDEVKHDH"
     gene            complement(418..1962)
                     /locus_tag="PLG52_07035"
     CDS             complement(418..1962)
                     /locus_tag="PLG52_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNB41237.1"
                     /translation="MKALNKRKLKFVLPLLVMLLAFVLVTPAFAQDGNPPTTEQVDDI
                     ALGVNMLWMLLGGFLVFFMQAGFALVETGFTRNKNVVHTMMMNMMVFCIGAVGYWLTG
                     FAFQFGGVNAAYPEVSTVGAIAGEWAHSPITLGDWSGLLATPLLRFGQFGLLGGSGFA
                     LAGVEANAGILAFFLFQMVFMDTAATIPTGSMAERLKFSGFVVMGLWVSMFIYPLVAG
                     WIWGGGWLQNLGRIAGLGNGAVDFAGSGPVHMIGGCVGLAGAIVLGPRIGKFNKDGSA
                     NSIPGHNITIGVLGTIILFFGWFGFNPGSSLGFLGGFRNLAVIAAINTLLAGAAGGVS
                     AMTYMWLFGPTKKPDAGMSVNGILAGLVAITAPCAFVAPWAAVVIGTIAGVLVIWAST
                     ALEKLKIDDPVGAVPVHMVNGAWGVLAVGIFANGNPDTAAWNGMESAVTGLIYGGTTQ
                     IVAQLAEVVSIFVVVVGLSWVFFKAMYALKLMRVSREDELAGLDLPEMGSLAYPVDWE
                     APADAV"
     gene            complement(1986..2456)
                     /locus_tag="PLG52_07040"
     CDS             complement(1986..2456)
                     /locus_tag="PLG52_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41238.1"
                     /translation="MYALSIVGYIMIALLLMNMMAKARPQIKEPMPGKALKSLPMGLA
                     SRFRLQRSSIMALLTGIVFGVVTGWLSLSTAGIVIAVALATIFLPMQYTFTTKGIALG
                     QGMFYPWSDFSGFTSKGSTLQFERSSVFGGLTVSIASTEVDTVLKYAERYIETR"
     gene            complement(2480..3784)
                     /locus_tag="PLG52_07045"
     CDS             complement(2480..3784)
                     /locus_tag="PLG52_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNB41239.1"
                     /translation="MIFTIIIFLLAYIAIASEKFPRHWVALLGGSLLILFGVLTPSEA
                     LSYISWETLGLVAGMFTLVSILVNAGFFTWLAMTSLRKVNYHPVPLFIILILLAAFLA
                     AFMDSITVMLFLSALTLQLCTLLKIDPVPLVIAEVCAANTGGAATLVGDPPNVILGTT
                     LGFSFADFATNTGPIAIVGALILMAYAYIKNRKSLKEAHIALTPEMIDQIDELHNEPL
                     HVHLTRVGLTGFMMAVILLIFHIPLSTLTGLPINAAVSALIPAGIALIALRDDQRKKV
                     IISVDGESILFFAGLFMLIGGLEKVHLFETLAESLALATASNPNSLIMALHWGPGLAS
                     GILDNVPLALAMTFVLKDLAKIPGTPALSMMVWSLAMGVDVGGNLTPIGASANVVAYS
                     YIEHNHGNIGWKRWLAIAVPPTLIVMIVTSIILLYKYQIGWY"
BASE COUNT         1180 a          976 c          975 g          910 t
ORIGIN      
        1 gtcaacgcca tggcattgat cgtaatcccc gttaacccaa gtgttttacg aactggcttg
       61 gaatcggact caatggtcat gctttacctc gtcgtctgat gaacctgatt tcaaccaggc
      121 ttcgaatttt tcaatgaaca ataccagggg ccagagcatg acgatacgcg ctccgttgac
      181 cagaaactga agccattgca gccaggtgaa tccgcgtgtg ttggtgccgt ctaacaagat
      241 gcgcgcgatc ataagcccca tgccaaggta tattaattca ggtggagaga tattggatgt
      301 tgtctcatgc atgacaatgc tgtactctat cttgaaattg gtatcgagac tccggaaggc
      361 ggcgatgtaa ttacccttcc gaagtctcga tgttgaaatt actggtgagt atagagacta
      421 tactgcgtca gcaggagctt cccagtctac agggtaggcg agagagccca tttcaggaag
      481 gtcgagacct gctaattcgt cttcgcgaga aacacgcatc aacttgaggg cgtacatggc
      541 tttgaagaat acccatgaga gacctacaac gaccacaaag atactgacca cttcagccaa
      601 ctgggctacg atctgagttg taccaccata gattagaccg gttacggcgc tttccatgcc
      661 gttccatgca gcggtatcgg ggttgccatt ggcgaaaatg ccgacggcga ggacgcccca
      721 agcgccattg accatgtgga caggaactgc accgacggga tcatcaatct ttaatttctc
      781 aagagcggtg gatgcccaaa ttaccaacac acctgcaata gtaccgatca caacagctgc
      841 ccacggagca acaaatgcac aaggtgctgt gatggcaacc aagccagcca aaataccatt
      901 gacggacatg ccggcatctg gtttcttggt gggaccaaac agccacatat aagtcattgc
      961 agaaacgcca ccagcggcac cagccaacag cgtgttgatg gcggcgataa cggctaagtt
     1021 gcggaagccg cccaaaaatc caagggaaga gccggggttg aaaccaaacc aaccgaagaa
     1081 caagataatg gttccaagca cgccaatggt gatgttatgt cccgggatcg aattggcgga
     1141 gccgtctttg ttgaacttac caatacgcgg acccagcaca attgcgccag ctaaaccgac
     1201 acaaccaccg atcatgtgga caggacccga accggcaaag tccacggcgc cattgcccaa
     1261 accagcgata cgacctaagt tctggagcca accaccaccc cagatccaac ctgcaaccaa
     1321 gggataaatg aacatactta cccagagacc catcaccaca aaaccagaga acttgaggcg
     1381 ttcagccatt gagccggtgg ggatggtagc ggctgtatcc atgaagacca tctggaataa
     1441 gaagaaggcg agaataccag cgttcgcttc tacaccagcc aatgcaaaac cacttccacc
     1501 gagaagacca aactgaccaa agcgcaacaa gggggttgct aaaagcccgc tccaatcgcc
     1561 aagggtgatc ggggagtgtg cccattcacc agcgatagcg ccaacagtgg atacttctgg
     1621 gtaggcagca ttcacaccgc cgaactggaa ggcaaagccg gttaaccagt agccaacagc
     1681 accaatacag aagaccatca tgttcatcat catggtgtgg acgacgttct tgttacgggt
     1741 gaagccggtt tccaccagag cgaacccagc ctgcatgaag aagaccaaga aaccgccaag
     1801 caacatccaa agcatgttga cacccagggc aatatcatcc acttgttcag tggtgggtgg
     1861 gtttccgtcc tgtgcaaaag caggtgttac aagcacaaaa gctaataaca tgaccaacaa
     1921 aggcaaaaca aactttagct ttcttttatt caatgctttc attttcattc tcctcttttg
     1981 agtttttatc ttgtttcaat atagcgttcg gcatacttca aaacagtatc cacctcggtg
     2041 gaggcgatgg atactgtaag acctccaaaa actgaagagc gttcaaattg tagagttgac
     2101 cctttgctcg taaaccctga aaaatcactc cacgggtaga acattccttg cccaagcgca
     2161 attccctttg ttgtgaaggt gtattgcatc ggtaaaaaga tggtggcaag ggcaactgcg
     2221 atgacgatcc ctgcagtgct gagcgataac caacccgtaa cgaccccgaa aactatccct
     2281 gtcaacaatg ccatgatgct tgagcgctgc aaacgaaaac gggacgccaa ccccatgggt
     2341 aggcttttca atgcttttcc tggcatgggt tctttgatct gtggacgggc ttttgccatc
     2401 atgttcatca acaacaaggc gatcatgatg taaccaacaa ttgaaagtgc gtacatgatt
     2461 tcttctctcc ttgaaaaaat tagtaccaac cgatttgata tttatagagc aagatgatcg
     2521 atgtgacgat catgacgatt aacgtaggcg gcacagcaat tgccaaccag cgcttccaac
     2581 cgatatttcc gtgattgtgt tcgatgtagg agtaagcaac gacattggcg gatgctccga
     2641 ttggagttaa attaccgcct acatcaacac ccatcgccaa tgaccaaacc atcatggaaa
     2701 gtgcaggtgt accggggatt tttgctaggt ctttgagcac aaaggtcatt gccaaagcta
     2761 gagggacatt gtctagaatg ccactggcaa gccctggtcc ccagtgcaat gccatgatca
     2821 aggaatttgg gttggatgca gttgccagag ccaatgactc agcaagagtt tcgaagagat
     2881 ggactttctc cagcccgcca atgagcataa agagaccggc aaagaataga atgctttcac
     2941 cgtctacgct gataatgact ttcttgcgct ggtcatctct taaggcgatc agtgcgatgc
     3001 cagcagggat caaagccgat actgccgcat tgatgggtag cccggttagt gtgcttaggg
     3061 gaatgtggaa gatcaagagg atgactgcca tcataaaacc ggttagcccg acacgagtca
     3121 ggtgaacatg aagcggttca ttatgcaatt cgtcgatctg gtcgatcatc tctggtgtga
     3181 gcgcgatgtg agcttctttc aacgatttgc gatttttgat ataggcatat gccatgagga
     3241 tcagcgcgcc gacaatggcg attggaccgg tatttgtggc gaaatcagca aaactgaacc
     3301 ccaatgtagt tcctagaata acattaggag ggtcgccgac caatgtggct gcgccaccgg
     3361 tgttggcagc acagacttct gcgatcacaa gtggtacggg gtcaatcttc aacaaagtgc
     3421 agagttgtaa tgtcaatgca gaaaggaaca gcattactgt aatgctgtcc atgaaggctg
     3481 ctagaaacgc agccaaaagg attaaaataa tgaataaggg cacagggtgg taattgactt
     3541 ttcgaagaga tgtcatggct aaccaagtaa aaaagccagc gttaaccaaa atggatacga
     3601 gggtaaacat cccagcaact aaacctagag tttcccagct gatgtatgaa agagcttctg
     3661 atggagtgag gacaccaaac aagattagaa gtgaaccacc caaaagggca acccaatggc
     3721 gtggaaactt ctcacttgcg atcgcgatat atgccaacaa aaagatgatg atggtgaaga
     3781 tcaaaggggc atctctcctc aaaagatgaa taacgtttta tgtaatgcgc cttattgtat
     3841 ggaattgaac ctgtatggct attaccctaa tgggtattca tttcatgttg tgctcaaaca
     3901 actatagatc acattgaaaa gatgccccaa atggagcacg tttaaatgac aaccgcgcca
     3961 tgaaacaatt tctcatggcg cagttgattt aatttcaccc gtttgggtac gttgttattc
     4021 ggtagcgaat ctctgatctt c
//
LOCUS       DAOQNJ010000204         4037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14342_length_4037_cov_8.133852, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000204 DAOQNJ010000000
VERSION     DAOQNJ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14342_length_4037_cov_8.133852"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..951
                     /locus_tag="PLG52_07050"
     CDS             67..951
                     /locus_tag="PLG52_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNB41240.1"
                     /translation="MNERRSFKIGMLFLLPWTIGFLAFTLYPMLASLVYSFTIYHPRQ
                     PLEWNGIKNYTDLLTDDLFWTALYNTLYMVLIGVPLTLFSSFFCAILLNLKVRGQAYY
                     RVIYFLPSIVPTVASTILWLWILNPQAGIMNTLLSYIGIDGPNWMTNPAWSKPGLILL
                     GMWGMGNTIIIYLSGLQDVPLSLLEAAELDGASWWQRLWNITVPMVSPITLFNLITGT
                     IFMFQYFAQAYVFRGFQGLGYPLNSTLFYSVLLYQNAFQWLKLGYASAMAWVLFIIIL
                     VCTLLMMKISDKFTYYAG"
     gene            956..2077
                     /locus_tag="PLG52_07055"
     CDS             956..2077
                     /locus_tag="PLG52_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNB41241.1"
                     /translation="MTNTNFPVQYPPHTPEPKKKISSRKIRDFFQSLFSHALINFIGL
                     FFLVPFLWMLITAFKSNQDVFHTPPRWLPYDNVLVDINGEQYPLYDVQTPDGVKQLAA
                     LKIVEGVGTFVDPASPTETFEYEMQQGNTKIAEPVMHVSFRWQNFPDAMNRASRPSID
                     ASFWVYFKNSLIIAFFSIVGTLLSNTPVAYAFARLKFPGRDFLFVIVLATMMLPYQVT
                     MIPLYLLFNNFLGWGDSFLPLIIPTFFANAYDVFLLRQFFRTIPEEMCDAARVDGASE
                     WQIFTQLVIPLSTPVLATVTVFTFLWAWNDFTGPLLFLTSPRNFTMALGLQDFQSQRT
                     LIWNQLMAASAIFTIPIIVAFFFAQKTFIQGIKLTGSKE"
     gene            2100..3491
                     /locus_tag="PLG52_07060"
     CDS             2100..3491
                     /locus_tag="PLG52_07060"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41242.1"
                     /translation="MTSTKITVIGAGSASFGENTLSAIMRSKKLSGSTLALVDRNTDS
                     LDIVRRLAERLNREWDSGFKIESYSHHRDALPDSRFVVCAIEVGARENLWQQDFEIPL
                     KYGVRQPYAENGGPGGFAHAARNIRPILDIAHDMEQACPDAWFINFTNPMVRICDAVN
                     RYSKIKVVGLCHQIYIGYCYVGLVLGDDLGFDIPKGIRNMNAEVDQHDLREQVIGQAV
                     NRLDIRAAGTNHFSWILSMVDRETGEDLYPLFRERYARFNPDFEPLTRRVNDAFGLMP
                     VPGDSHLCEYLPWLSNPETKPWEKYNIQLYNWDERAALRELELERLNKMANNELPIDS
                     LLESDSEGALEMIENITCAGTHYHLAANLPNVGQIPNLPLNTIVETPVIVNGAGIQPV
                     HTGELPLAVAELCRRELFVSQLGVDAAVEGSREKALQCLLLDPVITDIEQAKLILDDY
                     LTTYKEYLPQFWK"
     gene            3501..>4037
                     /locus_tag="PLG52_07065"
     CDS             3501..>4037
                     /locus_tag="PLG52_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB41243.1"
                     /translation="MAIKIAFAGTGYINKVHAQAAQNLGLDLVAVVNHKADSMREFGK
                     RFGIKRQYETVDAMLRDGDVDALVVSTPNYLHAPQTIAALQAGVHVMVEKPMAMNAHE
                     AEQMNRAAETSGALLMVAHCWRFDPDVLWLKEQAKKFGRILRTKGYGVHTHWGPSGWF
                     TQKQFAGGGAMADMGIHAL"
BASE COUNT          961 a          867 c         1007 g         1202 t
ORIGIN      
        1 gacatggaag aattgcgaac aacaggatgg aagttttcct ggcctcccaa atttaggaaa
       61 tggggcatga atgaacgacg cagtttcaag attggaatgt tgtttctgct tccgtggaca
      121 attggttttt tagccttcac tctttatccc atgctggcat ctcttgtgta cagctttacc
      181 atttaccatc cacggcagcc tttagaatgg aatggcatca aaaattacac tgacttgctg
      241 accgatgacc tcttttggac agcgctctat aacaccttgt atatggtgct gatcggcgtt
      301 ccgttgacgt tgttctcatc ctttttttgt gcaattctct tgaacctaaa agtgcgcgga
      361 caagcctatt accgcgtgat ttattttttg ccgtctattg tgccgaccgt tgccagcacc
      421 attttgtggt tgtggatttt gaatccgcaa gcagggatca tgaacacctt gcttagctat
      481 attggcattg acggaccaaa ctggatgacc aacccggcat ggtccaagcc ggggcttatt
      541 ttgctaggca tgtgggggat ggggaatacg atcatcattt atctttcagg cttgcaagac
      601 gtgccgctaa gcctgttgga agctgccgag ttggacggag ccagttggtg gcagagactt
      661 tggaatatta ccgttccaat ggtttcccca attacgcttt tcaatctgat tacaggcacg
      721 atctttatgt tccaatattt tgcacaagcc tatgtgtttc gagggtttca agggcttggg
      781 tatccgttga actcaacgct cttctatagt gtgcttttgt atcagaatgc ctttcaatgg
      841 cttaagctcg ggtatgcctc tgccatggcg tgggttttgt tcattattat tcttgtttgt
      901 acgttgctga tgatgaagat cagcgataag ttcacttact acgcagggta agccaatgac
      961 aaatacaaat tttcccgttc aataccctcc ccatacgccc gagccgaaaa agaaaataag
     1021 cagccgaaag attcgcgact ttttccagtc actgtttagc catgcgttga tcaacttcat
     1081 cggtttgttt tttctcgttc catttttatg gatgttgatt acggcgttta agtccaatca
     1141 ggatgttttt catactccgc ctcgctggtt accttacgat aatgttctgg tcgatataaa
     1201 cggcgagcaa tatccgcttt acgatgtgca gacgccggat ggtgtgaagc agttggcagc
     1261 actcaagatc gtggaagggg tgggcacttt tgtggacccg gcttctccaa ccgaaacgtt
     1321 tgagtatgag atgcagcaag ggaataccaa gatcgccgag ccggttatgc atgtcagctt
     1381 tcggtggcag aactttccag atgcgatgaa tcgcgcttct cgccccagca ttgacgccag
     1441 tttctgggtg tatttcaaaa atagtttgat cattgctttc ttttctatcg tggggacttt
     1501 gctatcgaat actccggttg catatgcctt tgcgcgtctc aaattcccag ggcgtgattt
     1561 tttgttcgtg atcgtcttgg cgaccatgat gcttccgtat caagtcacca tgattccttt
     1621 atatctcttg ttcaataatt ttctaggttg gggcgatagc tttttaccgc ttatcatccc
     1681 tacgtttttt gcgaatgctt atgacgtctt cctgttgcga caattcttcc gcaccattcc
     1741 tgaagagatg tgcgatgccg ctcgtgtaga tggagcttca gaatggcaga ttttcactca
     1801 actggttatt ccgctttcta cgcctgtgct tgcaacagtg accgtcttca cctttttatg
     1861 ggcttggaac gatttcacag gtccgctgct cttcttgact agcccgcgta actttacgat
     1921 ggcgctcggt ttgcaggact ttcaaagtca acgcaccttg atttggaatc agctgatggc
     1981 agcttcagcc atctttacga ttccgatcat cgttgcgttt ttctttgcac aaaagacctt
     2041 tattcaaggc atcaagttaa ccggttcgaa ggaataaggt tttaaacacg gaattgatta
     2101 tgacatctac aaagattaca gtaattggag cgggctcggc gtcattcgga gagaatactc
     2161 tctctgctat tatgcgttcc aaaaaattga gtgggtctac attggctctt gtggaccgaa
     2221 atacagatag cttagatatt gtccgcagat tggcagaacg ccttaatcga gaatgggatt
     2281 ctggcttcaa gatcgaatcc tattcacatc atcgtgatgc gttacctgac agccgttttg
     2341 tggtttgtgc cattgaggtg ggcgcgcgag aaaatttatg gcaacaagat tttgaaattc
     2401 cgctcaagta tggtgtgcgc caaccatatg ctgagaatgg tggacccggt ggttttgctc
     2461 atgctgcgcg gaatatccgt cctattttgg atattgctca tgatatggaa caagcttgcc
     2521 cagatgcatg gtttatcaat ttcaccaatc ctatggtgcg gatctgtgat gccgtcaacc
     2581 gatatagcaa gataaaagtg gtgggcttgt gtcatcagat ctatattggc tattgttatg
     2641 tggggttggt gttgggtgat gaccttggct ttgatattcc gaaagggatt cgcaacatga
     2701 atgcagaggt ggatcagcat gatttgcggg aacaggttat tggtcaggct gtgaatcggt
     2761 tggacatccg cgcggcagga acgaatcatt tttcgtggat tctgtcgatg gttgaccgcg
     2821 aaacggggga ggacttgtat ccgctttttc gtgaacgcta tgcccgcttc aacccagact
     2881 ttgagccgtt gactcgtcga gtgaacgatg ctttcgggtt gatgcctgtt cctggtgatt
     2941 cgcatttgtg tgaatacctg ccttggttga gtaaccccga aaccaagcct tgggaaaaat
     3001 acaacatcca actttataac tgggatgaac gtgcggcttt gcgtgaattg gaattggaac
     3061 gcctgaacaa gatggcaaac aatgaattgc cgattgattc gttacttgaa agcgacagtg
     3121 aaggtgcgct ggagatgatc gaaaacatca cttgtgctgg gactcattat catttggcgg
     3181 cgaaccttcc gaatgtgggg caaattccaa atttgccatt gaacactatc gttgaaactc
     3241 ctgtgatagt aaatggtgca gggattcaac ctgtgcatac gggcgaactt ccgcttgctg
     3301 tggcggagtt atgccggcgt gaactattcg tttcgcaact cggtgtggat gcggctgtag
     3361 aaggttcgcg tgaaaaagct ttgcaatgtc ttttgcttga cccagtgatt accgatatcg
     3421 aacaagcaaa attgatcctc gatgattact tgactaccta taaagaatac cttcctcaat
     3481 tttggaaata agaagatttt atggcaataa agattgcatt tgcaggtaca ggctatatca
     3541 acaaagtcca tgcacaagcg gctcaaaatt taggcttgga cttggtcgcc gtcgttaatc
     3601 acaaagcgga ctcgatgcgt gagtttggca agcgttttgg gattaagcgt cagtatgaaa
     3661 cagttgatgc catgctacga gacggagatg tggacgcgct ggttgtatcc acaccgaatt
     3721 atttgcatgc gccgcagacg attgctgcct tacaagccgg tgtgcatgtc atggtagaaa
     3781 agcccatggc gatgaatgcg cacgaagccg agcaaatgaa cagagctgca gaaacatctg
     3841 gcgctttatt gatggttgca cactgttggc gctttgaccc agatgtgttg tggttgaaag
     3901 aacaagccaa gaaatttggg cgtattcttc gcacaaaggg atatggtgta catacgcact
     3961 ggggaccttc tggttggttc acgcaaaaac aatttgccgg cggtggcgcc atggcagata
     4021 tgggcataca tgctttg
//
LOCUS       DAOQNJ010000205         4033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14364_length_4033_cov_7.660885, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000205 DAOQNJ010000000
VERSION     DAOQNJ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14364_length_4033_cov_7.660885"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLG52_07070"
     CDS             <1..248
                     /locus_tag="PLG52_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41244.1"
                     /translation="DVLREDYRVDYVLMTAEAYQTDFDADAAARRIAQLGSQAPAEIV
                     SSALTYAQKNGFTQDEQNALQTLLTAMQTYQPDGSQP"
     gene            245..1141
                     /locus_tag="PLG52_07075"
     CDS             245..1141
                     /locus_tag="PLG52_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41245.1"
                     /translation="MKQFLQNSILHIVLAVLIGFGLGLAYSWLLAPVTYVDAHPALLR
                     ADFKDQYRIIIAASYASNKDLPRAKARLDLLGDVDPVSELSAQAQRMLAAGESFDEAR
                     PLAQLATDLQQGYVSEPFTPTPFPTFNTPNAQFTSTPTESGDQPEVSEEATFTPAAPT
                     LLFEQTPITPIVQSTLTQRPTFTPTAGPGAPFALVNQETVCDLGMSRVMQFMLLNSKR
                     EQVPGAEIIVTWSQGEDRFFTGFKPELGNGYADFLMESNTTYNIRVVTGGAFVADVRA
                     PDCTDPNGATYSGGLVLTFQQP"
     gene            1205..1726
                     /locus_tag="PLG52_07080"
     CDS             1205..1726
                     /locus_tag="PLG52_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41246.1"
                     /translation="MNTSLPEQKPGLVNAIAWMTLASGIINLFWGFVASATALGTIVG
                     VICLPITILPTILGIFEIIYAAKLLSVQPQPVQPSNSIAVFEILTFLMGNVFSMVVGI
                     LSLIFYNDLTVKAYFARINAGNAVAPEPVIVSTPAQIEEPQPEILPEPIVEIIPEEPI
                     QPAKPKKPRKTSK"
     gene            1989..2654
                     /locus_tag="PLG52_07085"
     CDS             1989..2654
                     /locus_tag="PLG52_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41247.1"
                     /translation="MKSHRFFFGFMALLVAVSLACGGSGSPTANPTLPPVPTNPPAQQ
                     QDQGQQQQDQGQVPSDGTILTYTDQNNLWAFDLPGDWQHEYKDLGDTLYTDATAYLDT
                     FTSPDGSAYIDSLVMFANEGVTVNNSTSQAVALDLLNTYYSSTGKNNGDIRIKSDQIM
                     KDGSERFEWTSKGGNYSGISFFEVRGDDKRTWLMWTLVWNNTADQAVLDVIDSAIASY
                     RIP"
     gene            2733..3566
                     /gene="amrB"
                     /locus_tag="PLG52_07090"
     CDS             2733..3566
                     /gene="amrB"
                     /locus_tag="PLG52_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNB41248.1"
                     /translation="MMSSLDLRPSPIAGTWYPKDPEILANSIDAYLKEVQLLTLEGQV
                     LGVIAPHAGHRYSGAVAAHAFATLRGLTPDLVVVISPFHNFDPHPLLTTKHQAYATPL
                     GNIEVDQHTLNELRSHLQIPITPIAKDKEHSLEIELPFLQRVLKNDFKLLPIMVRAQE
                     ETVAKQLGEALAKTIQNRNAILVASTDLSHFYDQQTAQKLDQEMLNRFTTLNPTTIFE
                     AEQTGKGFACGHAAVATVQWVAKELGANNVQILKYATSGDITGDFQSVVGYGAAAILR
                     T"
     gene            complement(3609..>4033)
                     /locus_tag="PLG52_07095"
     CDS             complement(3609..>4033)
                     /locus_tag="PLG52_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41249.1"
                     /translation="LVAGALMILIALGLLAMVFMKSNQVNLTEKTDGKPEWMHAMPPK
                     ETVEATTKDGEGVTLYNYDEGEKVATPFAEQIEDVLRAKVESDPYLKSFTIDFGTAAD
                     GGLEIWVNGEKYDGVANLPDEQLKQSLLEAVKDWNSRK"
BASE COUNT         1064 a         1089 c          830 g         1050 t
ORIGIN      
        1 cagatgttct gcgcgaagat taccgtgtag attatgtgct catgaccgcc gaagcttatc
       61 aaaccgattt tgatgcagat gctgcagcac gccgcattgc tcaacttggc agccaagctc
      121 ctgctgagat cgtttcttct gcattaacct atgctcaaaa gaacggcttt acgcaagatg
      181 aacaaaatgc tttgcaaacc cttctaactg ccatgcagac gtatcaaccg gatggaagcc
      241 aaccatgaag caatttctgc aaaattccat tctgcacatt gtgctcgcgg ttcttatcgg
      301 gtttgggctt ggtctggcgt actcgtggct tctcgcccct gtcacctacg tggatgcgca
      361 tccggcgtta ttgcgcgcag atttcaaaga tcaataccgc atcatcatcg ccgcatccta
      421 tgcttcgaac aaagacttgc cacgcgccaa agcccggctg gacttactcg gagatgtaga
      481 cccggtcagt gaattgagtg cccaagctca gcgtatgctg gcagcaggcg aatcatttga
      541 tgaggcacgt ccgcttgcgc aattagcaac agaccttcaa caagggtatg tgagcgagcc
      601 gtttacgcct acacctttcc ccacttttaa cacaccgaac gcccagttca catccacgcc
      661 cactgaaagc ggagaccaac ccgaggtcag tgaagaggcg acgtttacac cagcagcgcc
      721 tacccttctc tttgaacaga ctcctatcac gcctattgtt caatccacat tgactcagcg
      781 ccctaccttc acaccaacag ccggacccgg cgctccgttt gcattggtca atcaagaaac
      841 agtttgcgac ctggggatgt ctagagtgat gcaattcatg ctcttaaatt caaaacgaga
      901 gcaagtgcct ggcgccgaaa ttattgtgac gtggagccag ggcgaagacc gtttctttac
      961 tggcttcaaa cccgagcttg gaaatggata tgcagatttc ttaatggaat ctaatactac
     1021 gtacaacatt cgggttgtaa ccggtggcgc atttgtggca gatgtgcgcg caccagattg
     1081 cacagacccc aatggtgcaa cctattcggg cgggcttgtg cttacattcc agcaacctta
     1141 atgatttgca accaatcgtg tgttcaatcg ttatattcaa caagcaaatc ttaaggagca
     1201 atacatgaat acctctcttc ctgaacaaaa gcccgggctt gttaatgcca ttgcatggat
     1261 gacgcttgcc agtggcatca tcaatctttt ttgggggttt gttgcatctg caactgcact
     1321 tggcacaatc gttggtgtca tttgtttacc gatcactatc cttcctacga tcttaggaat
     1381 tttcgagatc atttacgccg ccaaattatt aagtgttcaa ccccagcctg ttcaaccttc
     1441 aaactccatt gccgtttttg agattttaac gttcttaatg ggaaatgttt tctcaatggt
     1501 ggtgggtatt ctctctctga tcttctacaa cgatctcacg gttaaggctt actttgctcg
     1561 tatcaacgca ggaaatgctg tggcgccaga gccggtcatt gtttcaacac ctgctcaaat
     1621 tgaagaaccg cagcccgaaa ttctccctga accaattgta gaaattattc ctgaagagcc
     1681 aattcaacct gcaaagccta aaaagccacg aaaaacgtct aaataaattc atcttgaaat
     1741 tagctccaca gcatggaact ttttcacagc ctttgtcgtt tataggcagt caaaaaaatc
     1801 aagttccatg ctgtgatttt ttctagcatc ggattccctg ctttttcgtg gcttttctaa
     1861 tccaaattcc agatgtatgc aacgaatggc agggcgtgta aaatgggact aaggtacccc
     1921 tcaaaaaaat aaggtcatag ggtatccttg cgtccatcat caataaagat agaggagaat
     1981 acaaaattat gaaatcacat cgtttctttt ttggctttat ggcgttgtta gtagccgtca
     2041 gcctggcatg tggtggttcc ggttccccta ccgctaaccc caccttgccc cccgtcccca
     2101 ccaatccccc cgcccagcag caagatcaag gtcagcaaca acaggatcag ggacaagtgc
     2161 ccagcgacgg caccatcctc acctatactg accagaacaa tctgtgggca tttgacctcc
     2221 ccggtgactg gcagcatgaa tacaaagacc ttggcgacac cctttacacc gatgcaaccg
     2281 cctacttgga taccttcacc tcccccgatg gttccgcata catcgacagc ctcgtcatgt
     2341 tcgcaaacga aggtgtgacc gtgaacaact ccaccagcca ggctgtggcg ttggacctgc
     2401 tcaacaccta ctacagcagc accggcaaga ataacggcga cattcgcatc aagagtgacc
     2461 agatcatgaa agacggttct gaacgcttcg aatggacttc caaaggcggc aattactctg
     2521 gtatttcgtt ctttgaagtt cgcggcgatg acaagcgcac ctggttaatg tggactctcg
     2581 tctggaacaa cactgcagat caagctgtgt tggacgtgat cgacagcgcc atcgcttcct
     2641 accgcattcc gtaatatagc aagagagaaa gtagtactaa atccccgcga acattcgcgg
     2701 ggatttttta tttctccagg tgctacaata aaatgatgag ctctctcgac ctgcgcccct
     2761 ctcctattgc cggaacctgg tacccgaaag accctgagat attagccaac agcattgatg
     2821 cgtatctaaa ggaagtacaa cttctaacac ttgaaggtca agtactgggt gtgattgcgc
     2881 cacatgcagg gcaccgctat tctggggcag ttgccgcaca tgccttcgcc accctgcgcg
     2941 gacttacccc cgaccttgtt gtagtcatct ccccttttca taattttgac ccacatccgc
     3001 ttctgacgac caaacaccaa gcctatgcca ctccgcttgg caatattgaa gtagatcaac
     3061 atacacttaa cgaactacga tctcacttac aaattcccat tacacctatc gccaaagata
     3121 aagaacattc acttgagatc gaactccctt ttttgcaacg agttcttaaa aacgatttca
     3181 aacttttacc catcatggtt cgtgcacaag aagaaactgt cgccaaacaa ctaggggaag
     3241 ccctggcaaa gaccatccaa aaccgcaacg ccattctagt tgcttccaca gacctttctc
     3301 atttttatga ccagcaaacg gctcaaaaac ttgaccaaga aatgctaaat cgctttacta
     3361 cgcttaatcc cactacaatt ttcgaagctg aacaaacagg gaaaggcttt gcttgcggtc
     3421 atgccgctgt tgcaactgtt caatgggtag ctaaagaact tggtgcgaac aatgttcaaa
     3481 tcttgaagta tgcgacatcc ggcgatatca ctggagattt tcagtctgtt gttggctatg
     3541 gagccgcagc aatcttgcgt acctaacaaa aagcgccaga gaaatctctg gcgctttttt
     3601 ggacttttct attttctact gttccaatct tttactgcct caagcaaaga ttgcttaagc
     3661 tgttcatcgg gcaagttggc aaccccatca tatttttcac catttaccca aatctccaag
     3721 ccgccatcag cagccgtgcc aaaatcaatc gtgaacgatt ttaagtacgg gtcgctttcc
     3781 acttttgcac gcagtacatc ttcgatctgt tcggcaaatg gagtagcaac cttttcacct
     3841 tcatcatagt tatatagcgt tacaccttcg ccatctttcg tcgttgcttc caccgtctct
     3901 ttgggcggca tggcatgcat ccattcaggt ttgccgtcgg tcttttccgt caaatttacc
     3961 tgattcgatt tcataaacac catcgccaac aaccccaatg cgatcaaaat catcaaagcg
     4021 ccagcaacaa gaa
//
LOCUS       DAOQNJ010000206         4026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14401_length_4026_cov_10.580710, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000206 DAOQNJ010000000
VERSION     DAOQNJ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14401_length_4026_cov_10.580710"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLG52_07100"
     CDS             complement(<1..304)
                     /locus_tag="PLG52_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNB41250.1"
                     /translation="MIATDEKDINGLRAAGKVCAQAMHLMMESVKPGMTTLELDHIGE
                     NFLKKNGAKSAPRVMYNFPGGTCISVAPVIAHGIPGEHVIQDGELINIDVSAELNGY"
     gene            complement(305..802)
                     /locus_tag="PLG52_07105"
     CDS             complement(305..802)
                     /locus_tag="PLG52_07105"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNB41251.1"
                     /translation="MTEQWMPENKEELISAIEREWKLLISLAETLTDEQMTTLDEGGW
                     SPKDNLAHLTEWMNILMGYHMDKRPYYDVVGVSQEELPQWSVEAINPILFKRNQLRPR
                     AEVMDHLKRVYGLLVKKLDETPFEELLAPRHPDRPDPLILWVLGDTTEHFQEHRETIE
                     RGIKT"
     gene            complement(812..1498)
                     /locus_tag="PLG52_07110"
     CDS             complement(812..1498)
                     /locus_tag="PLG52_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNB41252.1"
                     /translation="MPSNLHLFSVPGERDLDDIVDACRPYVEGKEEPTIAYLPLASLY
                     AERWLEMTEDAFKGLAELKTINTELMTQKAIEGMIRDADVVYVPGGNTFLLNHRLHAS
                     GVMPFLQKRIQSGLPYVGFSAGAILCGPNILTSKDLNAVSTSFFKGLNAFQFNVSPHY
                     PLDGYGQSVKDDWLADYHFFHDNPVIMLCDGSYIKSEKGKTSLVRGEAYILRKDSEKE
                     RLEEGQIITL"
     gene            complement(1551..2165)
                     /gene="tmk"
                     /locus_tag="PLG52_07115"
     CDS             complement(1551..2165)
                     /gene="tmk"
                     /locus_tag="PLG52_07115"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNB41253.1"
                     /translation="MFITLEGPEGSGKTSHIPHLVEFLREKGYTVFPTREPGGTSISE
                     QIRDVLHDMKNAEMHARTETLLYQAARAQIVEQVIKPRLADGEIVISDRYYDSTIAYQ
                     GYGHQQNLDEIRALVKYATGGLTPDLTILLDLDVEVGLGRKKKDNEWNRLDAYTVEFH
                     RRVRAGYQEMVKAEPQRWAVVNSEQAWDSVQAELRQVILEKLGK"
     gene            2331..3479
                     /locus_tag="PLG52_07120"
     CDS             2331..3479
                     /locus_tag="PLG52_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNB41254.1"
                     /translation="MSKKNIVYLFLVVVIAGASALTGAAAGGFAVYQAIGVKQNNQKS
                     AAETAAKLEQVSQSLVLNATDVETAVTQAVQNVSPAVVTVVGTVQGQTNFFGQSSDAT
                     VSGSGFFISEDGYVLTNNHVVEGTQEVKVVLADGTEQTAQIVGTDRYSDIAILKTDGK
                     VPAVAKLGDSDLLQPGESVIAIGSPLGDFKNTVTVGVVSATGRTIDSGQGYQIEGLIQ
                     TDAAINHGNSGGPLVNLAGEVVGINTLVVRNSGSSSDVAEGLGFAIPVNTAQVIAQQI
                     ITNGYVSRPFMGINYQAISPNIAAMYNLPVKWGIYVQAIADGSPASTSGLQKGDIIAS
                     LNGVQLDETHQYLNMLYNYKPGDTVTLGIYRDGKQLDLTITLGENNPT"
     gene            complement(3527..>4026)
                     /locus_tag="PLG52_07125"
     CDS             complement(3527..>4026)
                     /locus_tag="PLG52_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNB41255.1"
                     /translation="TPSQVPNVGAKLQTIRIFAYGVARNRLQQAAKRLGVPAVVVTDE
                     AEADVLVTLRTYYRNREHTIMDAEARGMPIYVLRANSVSQVEQFVSDLYNLTEHVQRD
                     NMEDIRDQAKQAISAVLNGERFVDLPPGPSLVRRLQHEMARDAELVSHSYGKEPRRHV
                     RIFRE"
BASE COUNT          987 a         1020 c          987 g         1032 t
ORIGIN      
        1 agtagccatt cagctcagca gagacatcaa tgttgatcag ttcgccatct tgaatgacgt
       61 gctcacccgg aataccatgt gctatgaccg gcgcaacgct gatgcaagtg ccgccgggga
      121 agttgtacat tacacgcggc gcagacttgg cgccattttt cttaagaaaa ttttcgccga
      181 tatggtctag ctcgagcgtg gtcatgcccg gtttgacact ttccatcatc aaatgcatgg
      241 cttgggcgca gactttgcca gcggctctta agccgttgat atctttttcg tcagttgcga
      301 tcatttaggt tttgattcct ctttcaatag tctcacgatg ttcttgaaaa tgttcggtcg
      361 tatcgcccaa gacccataag atcaaagggt cgggtcggtc tgggtggcgc ggagcaagca
      421 attcttcaaa gggagtttcg tctaattttt tcaccagaag tccatagacg cgttttaggt
      481 gatccatgac ttctgctcgc gggcggagtt gattgcgctt aaacaagatg ggattaatcg
      541 cttctacgct ccactgaggc aactcttctt gcgagactcc aacgacgtcg taatatgggc
      601 gtttgtccat atgatagccc attaagatat tcatccattc ggtgaggtga gcaaggttgt
      661 ctttgggtga ccagccgcct tcgtcgagtg tggtcatttg ttcatcggtt agcgtttcgg
      721 caaggctgat cagcaacttc cactcacgtt caatggcaga aatcagttct tctttatttt
      781 caggcatcca ttgttcagtc atcgcttcct cctataacgt gattatttga ccttcttcaa
      841 gtctttcttt ttctgagtct tttcgcaaaa tgtacgcttc ccctcggaca aggctggtct
      901 tgcccttttc gcttttaata tatgatccat cgcacagcat gataacaggg ttgtcgtgga
      961 agaagtgata atctgccagc caatcatcct tcaccgactg accatatcca tccaacggat
     1021 aatgcggcga gacattgaac tgaaatgcat tcaagccttt gaaaaaggat gtgctgactg
     1081 cgttcaaatc tttcgaggtt aagatgtttg gtccacacag gatagcgccc gcactaaacc
     1141 caacataagg caaaccagat tgaatgcgct tttgcaggaa aggcattacc ccgctagcat
     1201 gtagacgatg gtttagcaaa aaggtattcc caccaggcac atacacaacg tctgcatcgc
     1261 ggatcatgcc ttctattgct ttttgggtca tcaactcggt gttgatcgtt ttcagttctg
     1321 ccaacccctt gaaggcatct tcggtcattt ccaaccaacg ttcggcatat aaagaagcca
     1381 acggcaaata ggcaatggtc ggttcctctt tgccttccac atatggacgg caagcatcga
     1441 cgatatcgtc taagtcccgt tctccgggga cagaaaagag atgaagattg gatggcataa
     1501 cggggattgt atcaaagatg taggggcggg gtgaccccgc ccctacgaca ttatttacct
     1561 aatttttcta aaatcacttg cctcaactcc gcctgcacgg aatcccatgc ttgctccgag
     1621 ttgaccactg cccaacgttg cggctcagcc ttcaccattt cttgataccc ggcgcggaca
     1681 cggcgatgaa attcaactgt gtaagcatcc aggcgattcc attcgttatc cttcttcttt
     1741 ctccctaaac caacttctac atcgaggtcg agcagaatgg tcaaatctgg agtcaacccg
     1801 ccggtggcgt atttaaccaa ggcgcggatt tcatccaagt tttgctggtg accgtagcct
     1861 tgataggcga tggttgagtc atagtaacga tccgaaatga cgatctcccc gtcggcaaga
     1921 cgcggtttga tgacctgctc cacgatctgg gcgcgtgcgg cttgatagag caacgtctcc
     1981 gtgcgagcat gcatctctgc attcttcata tcatgcaaca catcgcgaat ctgttcgctg
     2041 atggacgtcc cgccgggttc gcgggttgga aagacggtat agcccttctc gcgtagaaac
     2101 tcaacaaggt gtggaatatg ggaggttttg cccgagcctt cgggaccttc aagagtaatg
     2161 aacatggggc aagtataaag tagacaagtt aacaagtaaa caggaaaacg agccggttaa
     2221 gtgtttacat ttttcctaca tctttcttaa cctgctttca gtttgaggtg aaaattttta
     2281 atcatcgtgt cattttcgag gactacgatt ttttcaactt ggaggcatga atgtcaaaga
     2341 aaaatattgt ctatttgttt ctggttgtcg tgattgctgg cgcctcagca ctgacgggtg
     2401 cagcggctgg cgggtttgct gtctatcagg caataggcgt gaagcagaac aatcaaaaat
     2461 ccgctgctga aactgcggca aagctggaac aagtcagtca atccttggta ttgaacgcaa
     2521 cggatgtgga aaccgccgtt acgcaagcgg tgcaaaatgt ttcccccgca gtcgtcaccg
     2581 tagtgggaac cgttcaaggg cagaccaact tttttggtca gagcagtgac gccacggtca
     2641 gcggaagcgg gttcttcatc tcagaagatg gttatgtgtt gaccaataac cacgtagtgg
     2701 aaggaacgca ggaagtaaaa gtggtgcttg ccgatggcac cgaacagacc gcccagattg
     2761 ttggcacaga ccgttattca gatatcgcca ttctcaaaac ggatggaaag gttccggcgg
     2821 tggcgaaatt aggagactcg gacttgctgc aacccggtga atcagttatt gcgatcggct
     2881 ccccgctcgg cgacttcaag aataccgtga ccgttggtgt ggtcagcgca accgggcgca
     2941 ccatcgactc gggtcaaggc taccagatcg aaggcttgat ccaaactgat gccgccatca
     3001 atcacggcaa ctcgggtggt ccgctggtga acttagcggg tgaggtggtt ggcatcaata
     3061 cgcttgtggt tcgtaattcc ggttcaagca gtgatgtagc cgaaggcttg ggctttgcca
     3121 tcccggtcaa cacggcgcag gtcattgcgc aacagatcat caccaatggc tatgtctcac
     3181 gtccgttcat gggcattaat tatcaggcga tctccccgaa catcgccgcc atgtataacc
     3241 tgcctgtgaa atggggcatc tacgtccaag ccattgcaga tggaagccct gccagcacat
     3301 caggattaca aaaaggcgat atcatcgcca gcctgaacgg tgttcaatta gacgaaacgc
     3361 atcagtactt gaacatgctc tataactaca aaccgggcga taccgtcacc ctcggcattt
     3421 accgtgatgg caaacaactc gacctaacca tcactctcgg cgaaaacaac ccaacataaa
     3481 gcttctttat aaaaatagag gtgacctgta caggtcacct ttatttttat tctctaaaaa
     3541 ttctcacatg cctacgcggt tctttcccat aggaatgaga aaccaattca gcgtcacgcg
     3601 ccatttcatg ttgcaaacgg cgcacgagag aaggtccggg cggcaggtca acgaaacgct
     3661 cgccattcaa gaccgcacta atggcttgct ttgcctgatc acgaatgtct tccatgttgt
     3721 cacgttgcac atgctcggtc aggttataca agtcactcac gaactgctcc acttgtgaga
     3781 cagaattcgc acgcaagaca tagatcggca taccacgcgc ctcggcatcc atgatggtgt
     3841 gctcgcggtt acggtaatac gtgcgcagcg tcaccaacac atcggcttcg gcttcatcag
     3901 tcaccactac ggcgggcacg ccgagtcgct tcgccgcttg ctggagtcga ttgcgcgcca
     3961 caccataggc gaagatgcgg atggtctgca attttgcgcc aacgttcgga acctgacttg
     4021 gcgtag
//
LOCUS       DAOQNJ010000207         4020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14438_length_4020_cov_10.202018, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000207 DAOQNJ010000000
VERSION     DAOQNJ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14438_length_4020_cov_10.202018"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..315)
                     /locus_tag="PLG52_07130"
     CDS             complement(94..315)
                     /locus_tag="PLG52_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41256.1"
                     /translation="MAKKVWQVEKTKYCEHLQQAIAIEVEVVYPAEQLPEQTPRVMAR
                     RCSKAIECNCVEHPSCSLSGTNPDLDPVE"
     gene            complement(426..1646)
                     /locus_tag="PLG52_07135"
     CDS             complement(426..1646)
                     /locus_tag="PLG52_07135"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNB41257.1"
                     /translation="MRRLFLPLLLTVLACGGNGIPGPTPTPLSPTATVSAPQTESQPP
                     ADPATLPPTAVVIESAQQFPDPTAYEWKPLVEGLDRPVDLQSAQDGTGRLFIIEKYGA
                     IRIFKDGQLSLQPFLNIDDRVNDDSNEMGLLGLAFHPNFEQNGYFYVNYTGDGGNPRI
                     SRFTANGDVADPNSEQVLMVIDQPFPNHNGGAVAFGPDGYLYLGLGDGGAAGDPFKNG
                     QNTETLLGKLLRIDVNNGDPYSIPVDNPFGNEVWAYGLRNPWRISFDRATGDLWIGDV
                     GQNQYEEIDYLPAGSPGGANFGWSVMEGLHGYDGEAQPGLLLPVVEYSHEIGCSVTGG
                     YVYRGSMPEWNGVYLYGDYCSGSVSGLILVDGQWQSRVLFEAVATITSFGQDEAGELY
                     FTSDNGSIYILAKK"
     gene            complement(1647..2018)
                     /locus_tag="PLG52_07140"
     CDS             complement(1647..2018)
                     /locus_tag="PLG52_07140"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HNB41258.1"
                     /translation="MTEKKSSDIIVNQQGGVVRNLVNQLKLIFRLMGDKRVSVLAKLV
                     PLGAFAYLIMPADLAPNVVLPVIGMVDDAAILWLGTYIFTELCPPEVVEEHMKALAGN
                     MNPKDVNNEDVVDGETTEVKE"
     gene            2238..2630
                     /locus_tag="PLG52_07145"
     CDS             2238..2630
                     /locus_tag="PLG52_07145"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB41259.1"
                     /translation="MDMIIDFPGGQKVDAHFRGHTIKTDQPNDNTAPMPFELFLASIG
                     TCAGIYVLGFCQQRNLPTAGIRIVQRNYSNPANGLVDKIDLEIQVPPNFPEQYHAALV
                     RTAELCKVKKTLEHPPVFDVMTKVVEAA"
     gene            2715..3182
                     /locus_tag="PLG52_07150"
     CDS             2715..3182
                     /locus_tag="PLG52_07150"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNB41260.1"
                     /translation="MPYIIDGHNLIPKLGLRLDEPDDEMELVRLLQDFARIRRQQIDV
                     YFDGAPAGQAGMRKFGSIKAHFVRQGQTADSAIRRRLEEMGKAAKNWIIVSSDHEVQG
                     AARVHMAQAIRSEEFVKQIRIAFSTSTQTGASDSKLSAQEVEEWINLFNKKKE"
     gene            3417..>4020
                     /locus_tag="PLG52_07155"
     CDS             3417..>4020
                     /locus_tag="PLG52_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41261.1"
                     /translation="MMVSNDVVVQRSYVYFVRWCMAVVITMGVFACSYIPSLPSPTSF
                     TECIPPSYTANIYRPTPTPTFPVAVASTSIPNIVSTAQPMPVDIHLHFQKLIQEVSRW
                     TAITPFPLGDSSEVWVAITFLSPELLRTVSTTDLLSENPAGINLDIQVNNMLAEIAKR
                     EKLLFFLTAFKANTGGEATNSHTFQIDVENLKLINAKNLGI"
BASE COUNT         1068 a          992 c          945 g         1015 t
ORIGIN      
        1 tttaacggct tacgccgcgc gttgcgcggc gtagccttaa gaccaactca gagggaggag
       61 agagagtttg gatgaacagc gttgtgcgct gctctattca acagggtcaa ggtcaggatt
      121 tgtcccgctt agagagcaag aggggtgttc tacgcaattg cattcgattg cttttgaaca
      181 gcgccttgcc ataacacggg gagtttgctc gggcagttgc tcagccgggt atacaacctc
      241 gacttcgatg gcgattgctt gctgcaagtg ctcgcaatat ttggtttttt caacttgcca
      301 aacctttttt gccataactg ctcctgactc agatgtccct agtttagaac caaaacgcct
      361 gtcagaccag tgacaggcgt cataaactcg gatgacaaag cacctattat ggatgtgata
      421 agtctttatt tctttgcaag gatgtaaata ctgccgttat cgctggtgaa gtacaactcg
      481 ccagcctcgt cttgcccaaa agaggtgatg gttgccaccg cctcaaacag gacccgagac
      541 tgccactgcc catcgaccag aatcaaccct gaaacagagc cagaacaata gtcgccgtac
      601 aagtacacgc cgttccattc tggcatggag ccgcgataga catatccgcc tgtcacagag
      661 cagccaattt catggctgta ttcgactacc ggtaacagta gccctggctg ggcttccccg
      721 tcgtacccat gcagcccttc cattacactc cagccaaagt tggcaccgcc tggcgagccg
      781 gctggcaaat agtcaatctc ttcatattgg ttctgcccca catcgccgat ccacagatca
      841 cccgtagcgc ggtcaaatga aatgcgccac gggttacgaa gcccatatgc ccagacctca
      901 ttgccgaatg gattatccac agggatagag tatgggtcac cattgttcac gtcaatgcgc
      961 aggagttttc cgagtagggt ttcggtgttc tgcccattct tgaacgggtc acctgcagca
     1021 ccgccatcgc ccagcccgag atagagataa ccgtcgggtc cgaaggctac agcgccgcca
     1081 ttgtgattcg ggaaaggttg gtcgatgacc atcaacacct gttcgctgtt ggggtcggca
     1141 acgtcgccat tggcggtaaa gcgcgagatg cgggggtttc cgccatcgcc tgtgtagttg
     1201 acgtagaaat agccattctg ctcaaagttt gggtgaaacg ccaagcccag tagccccatt
     1261 tcattactat catcattgac acggtcatca atgttaagaa aaggttgaag agaaagctgc
     1321 ccatctttga agatacggat ggcaccatat ttttcgatga tgaacaagcg cccagtgcca
     1381 tcctgcgcag actgtaaatc tacggggcga tccaagcctt ccacgagagg tttccactcg
     1441 taggcggttg ggtcggggaa ttgctgggcg ctttcaatca ccaccgcggt tgggggaaga
     1501 gtggcagggt ccgctggcgg ttgagattcg gtttgtggtg ctgaaacagt tgccgttgga
     1561 gaaagaggcg tgggggtggg tccgggaatg ccattccctc cgcaggcaag gacggttaac
     1621 aataaaggga ggaaaagacg tcgcatttat tcttttactt cagtggtttc gccatccaca
     1681 acatcttcat tgtttacgtc tttggggttc atatttccag caagcgcctt catgtgctct
     1741 tccacaactt ctggcgggca caattcggtg aagatatagg taccaagcca gagaatggcg
     1801 gcatcatcga ccatgccaat tacaggcaag acgacgttcg gcgcaagatc agcaggcata
     1861 atcaaataag caaaagcgcc caagggcact aactttgcca agacactgac gcgcttatcg
     1921 cccatgagac gaaagataag cttcagttgg ttcacaaggt tgcgcaccac cccgccttgt
     1981 tggttgacga tgatatcaga ggattttttc tcagtcattt tgttctcctt tgagttgtat
     2041 gttcattata ttcggaattg cagtacatcc actttcgatt attaccgttt gcatgaacat
     2101 agattgatat acgtgacaaa agaagatggg gtgcaggtaa aaatggcaga agcaggaaaa
     2161 aaactcaaaa attgggtgta acttattctt agcaaataca tccaatatgt acaacaataa
     2221 atttggagga tttccccatg gatatgatta tagatttccc cggcgggcaa aaagtagatg
     2281 cccacttccg cggtcacacc attaagacag accaacccaa cgacaatact gctccgatgc
     2341 cgtttgaatt gttccttgct tctattggta cctgtgcagg catttatgtg ctaggctttt
     2401 gccaacagcg caacctgccc actgcaggca ttcgcatcgt tcagcgcaat tattccaacc
     2461 cggcaaacgg gttggtcgat aagatcgatc ttgaaattca ggtaccaccc aacttccccg
     2521 aacaatacca tgccgcactc gtgcgcactg cagagttatg caaagtgaag aagactcttg
     2581 aacatccacc tgtgtttgat gtaatgacca aagtggtcga agctgcctaa gaaaccgaag
     2641 ataaaaagac cgcagacatt cacagtctgc ggtcttttta ttccaaaaca actcaagcac
     2701 agtaaaatga acccatgccc tacattattg atggacataa cctgattccc aaacttggat
     2761 tacgtttgga tgaacccgat gacgaaatgg aactcgtgcg cctcttacaa gactttgcac
     2821 ggattcggcg acagcagatt gatgtctact ttgacggcgc tcccgccggg caagctggca
     2881 tgcgaaaatt tggcagcatc aaagcgcact tcgtaaggca aggtcaaact gcagattcag
     2941 ccatccgcag gcgattagag gagatgggga aagcagcaaa aaattggatc attgtttctt
     3001 cagaccatga ggtgcaaggt gcggcacgag tgcatatggc acaagcgatt cgctcggaag
     3061 aatttgtcaa acaaattcgc attgccttct ctacaagtac ccaaacgggt gcatctgact
     3121 caaagctttc ggcgcaagaa gtggaagagt ggataaatct atttaacaaa aagaaagaat
     3181 agtacctttg tcttatacgt ttttaatgaa atctggacaa aaaaggtgta tattgtctgt
     3241 acgacacctg ccctcccttt tactggtatt ctggtgtcaa ccgccgctct ggtcaacatg
     3301 ccccccccat gctgacaggc ggcggtacct ttttaacaat gccaaaatac acttgaagaa
     3361 ggtctgagag gtcaatataa tattttctat ccgatcaaat caatgtggag gcgggcttga
     3421 tggtttcaaa cgatgtagtg gtacagcgtt cttatgtgta ttttgtacga tggtgtatgg
     3481 cggttgtgat cacaatgggg gttttcgcat gcagctatat tccttcttta ccatccccca
     3541 cctcttttac agaatgtatc cctccatctt atacagctaa tatctatcgt ccaacaccaa
     3601 cgcccacctt tcctgttgca gtagcatcca catcgattcc caatattgtt tcgactgctc
     3661 agcccatgcc agttgatatc catctgcatt ttcaaaagtt aattcaagaa gtcagccgct
     3721 ggacggcgat aactcctttc ccacttggcg attcaagtga agtttgggtt gcgatcacat
     3781 ttctaagccc tgagctttta cgaaccgtct ctactaccga ccttttgagt gaaaacccag
     3841 ccggtatcaa tttagatatt caagttaaca atatgcttgc agagattgcc aagcgcgaga
     3901 aactattatt tttcttaaca gccttcaagg cgaacacagg tggcgaagcc acaaactcgc
     3961 acacgtttca aattgatgta gaaaatctaa agcttatcaa tgccaaaaac ctcggtatta
//
LOCUS       DAOQNJ010000208         4001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14556_length_4001_cov_9.020020, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000208 DAOQNJ010000000
VERSION     DAOQNJ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14556_length_4001_cov_9.020020"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1774
                     /locus_tag="PLG52_07160"
     CDS             <1..>1774
                     /locus_tag="PLG52_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNB41262.1"
                     /translation="IRSELEKLYDAQTIYRSGFVVYTTLDPVLQDRAQELVTNQVAAM
                     VNNNTKNGALVAIQPSTGQILAMVGSPDFNNAAISGQINMAISPTRQPGSSIKPITYV
                     TAFEKGWTPATWIWDVPTQFPDGANAPYEPRNYDGTFHGGLTVRLALANSFNIPAVKA
                     LEYVGIYDDPNTPEKDGMIAMAERLGITSLTRTDYGLSLTLGGGDVSLFDMTSAYAVF
                     ANGGKKLPPVAILKITTFDGEVVYEYQQPEGEQILRPEHAYLISSILSDNEARSYTFG
                     RNSALNLSFPVAAKTGTTNDIRDNWTMGYNPDLVTGVWVGNADYTPMVNSSGLSGAAP
                     IWSQFMEFAVPYLTNGAPTPFSRPSGIVDKVVCRLGGTEPTNECGGEYTEVFASDQLP
                     PTKDQGLVRRVNLDVWTGYEASDACDGPTDEKLVMNVKDPWALKWFDTGDGKAWLADH
                     GLDENLDIVPDRKCEEGDPQPEIEINLSDGQVISAPVVEVKGTASASGNFKQWRLEFG
                     IGEDPGSWTTLSESNNPVESGTFTTWDVSNLPNGIITLRLTLVGERTDISKRIRLNLS
                     LPIPTVPSATPTLTATPTETPTLI"
     assembly_gap    1775..1874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1984..2694
                     /locus_tag="PLG52_07165"
     CDS             1984..2694
                     /locus_tag="PLG52_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41263.1"
                     /translation="MNTMNCPNCGAAISAAALKADIATCEFCGTSFRLPKTLTPEPDM
                     GDLLLGADFSQKTIPGWETVNDDKLHFHKGPPAELRGKFTPQVNSYYMLKSSGFLDNF
                     DVSSTLRFFDGDKEWIRAGYFFRHTPEGGYGLMVSAQGSYTFGYVAKDEKNELLFYKI
                     MPWSWHTALRSGMNVFNRLRVICNGSSFRIYLNGVLATSFQDERFKMGRIYLAAEPSE
                     KSSMELGFSDLQVREVPK"
     gene            2999..>4001
                     /locus_tag="PLG52_07170"
     CDS             2999..>4001
                     /locus_tag="PLG52_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNB41264.1"
                     /translation="MSWSYGEVLKPETINYRRLRPEKDGLFCEAIFGPTRDWQCYCGK
                     YKNPRYKGITCEKCGVEVTRSSVRRERMGHIGLASPVAHIWYTRRIPSQMGMLLDISR
                     RNLDRVLYFAQYIVTYVDEEARKKALQRLEDEITVSEREQASEINNRIIEIKQKRDAS
                     ISELKQKQATLEQGYDEGIAEQLDPVIKEGQKLERLLQDQMGEAAKKAIVFEQTGEKI
                     IDAGDKVASKHITLIQKTVQKKLESLENELKDKRQAEIDDLKTKAATIKAQADTEMEA
                     LRNQLESQTSVSSNNSSRLRDELLELRPFTFLSEIRYRELKQRWGQVFRADMGAEAFY
                     "
BASE COUNT          988 a         1040 c          945 g          928 t
ORIGIN      
        1 tcatccgttc ggaattggaa aaactatacg acgcgcaaac catctatcgc tctggctttg
       61 tcgtatacac cacgctcgac ccggttcttc aagaccgcgc acaggaattg gttaccaacc
      121 aagttgctgc tatggtgaac aacaatacca agaacggcgc cttggtggcg attcaacctt
      181 ctaccggtca gattcttgcc atggtcggct cgccagattt caataacgcg gcgatttccg
      241 gtcagatcaa tatggcgatc agccccacgc gtcagcctgg ttcgtcgatc aagcccatca
      301 cctatgtgac tgcctttgaa aaaggttgga cccccgccac atggatctgg gatgtcccca
      361 ctcaatttcc agacggtgcc aacgccccct atgaaccgcg taactacgat ggcaccttcc
      421 acggtggact caccgtccgc ctcgccttgg cgaactcctt caacatccct gccgtcaaag
      481 cattggaata tgtcggcatt tacgatgacc cgaacacccc cgaaaaagat ggcatgatcg
      541 ccatggcaga acgccttggc atcacatcgc tcacgcgtac cgattatggt ctgtccctca
      601 ccttgggcgg cggcgatgtt agtctcttcg atatgaccag tgcctatgca gtctttgcca
      661 atgggggcaa gaaactcccg ccagtcgcca ttcttaagat caccaccttt gacggggaag
      721 tggtttatga atatcaacag cccgaaggcg aacagatcct tcgtcctgaa catgcctatc
      781 tcatctcctc cattctttca gataacgaag cgcgcagcta cacctttggt cgcaactctg
      841 cattgaatct ttccttcccg gtagcagcta aaaccggtac caccaacgac atccgcgata
      901 actggacgat gggctataac ccagacttgg tcacaggcgt gtgggttgga aatgctgatt
      961 acacccccat ggtcaattca tccggcttga gcggagccgc tccgatctgg tcacagttca
     1021 tggaatttgc cgtgccctat ctcaccaacg gcgcgccgac tcctttctcg cgtccatctg
     1081 gcatcgtgga taaagttgtc tgtcgtctcg gcggaaccga acccaccaat gaatgcggcg
     1141 gcgaatatac cgaagtcttc gcatcagacc aattgcctcc cacaaaagat cagggacttg
     1201 tacgccgcgt aaacctggac gtttggactg gctatgaagc ctcggatgca tgtgatggtc
     1261 ccaccgatga aaagctcgtc atgaacgtca aagacccgtg ggcgctcaag tggttcgaca
     1321 caggcgatgg aaaagcctgg cttgcagatc atggcttgga tgaaaatcta gacatcgtgc
     1381 cagaccgcaa gtgtgaagaa ggtgacccgc aaccagagat cgaaataaac ctctccgatg
     1441 gtcaagtcat ttctgccccc gtggttgaag tcaaaggcac tgcctctgcc agcggtaact
     1501 tcaaacaatg gcgtttagaa ttcggcatcg gcgaagaccc cggttcatgg acgactctct
     1561 cagaaagcaa caaccctgta gagagcggca ccttcaccac ttgggatgtt tcgaatctgc
     1621 caaacggaat cataaccttg cgcttgactc tggttggaga acgcacggat atcagtaaac
     1681 gcatcagact taatttgagc ctgcccattc caactgtgcc ttctgccacg ccaacgctga
     1741 cagcaacccc caccgaaacg ccgacgttga ttatnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnncttctg ccacgccaac gctgacagca acccccaccg aaacgccgac
     1921 gttgattatt gttattccac ccagtgaaac acccactgaa acacccacct tgactcctac
     1981 gccatgaaca cgatgaattg tcccaactgt ggtgcggcaa tatctgccgc tgccctcaaa
     2041 gcagatattg ccacctgtga attttgtggc accagttttc gcttgccgaa aactctcacc
     2101 cccgagccag acatgggtga cctactcctt ggcgcagact tttcccagaa aaccatacct
     2161 ggttgggaaa cggtcaacga cgacaagtta catttccata aaggtccgcc cgccgagttg
     2221 cgcggaaaat tcacgccaca ggtcaactcg tattacatgc taaagagttc tggcttttta
     2281 gataacttcg atgtaagttc taccctgcgc ttttttgatg gcgataaaga gtggattcga
     2341 gcggggtact tcttccgcca tactcctgaa ggtgggtatg ggttgatggt gtcggcacag
     2401 ggttcgtata cgtttggtta tgttgccaaa gatgaaaaaa atgaattgct tttttataag
     2461 atcatgccat ggtcgtggca tacggcgtta cgttctggca tgaacgtctt taaccgcttg
     2521 cgagtgatat gcaatggcag cagttttcgc atctacttga acggtgtgct tgccacgtcg
     2581 tttcaggatg agcgtttcaa aatgggtagg atctaccttg ccgctgagcc aagcgagaag
     2641 tcttctatgg agttgggatt ctccgatttg caggtgaggg aagtgccaaa ataggcagaa
     2701 tcaggctttg gtacgggaaa gaggcgctga atccgcctta aagtatgcct ctcttcactg
     2761 attaaccttg actctcagtg cctgaatcca tataattgct aagttggatg taaaaaacga
     2821 gaaaacacca gcaagcgctt gaggaaaaac ctcagagcgc ttgcatttgt ttgtctcagg
     2881 tacgcgttat ctatgcgcta ccgtatcgct caaacgaaat ttattttttg gagaggatcc
     2941 agtggaaacc aagggattaa cagcactccg tatcagcctt gcctcacccc agacaattat
     3001 gtcctggtct tatggcgagg tgttgaaacc cgaaaccatt aactatcgcc gcttgcgccc
     3061 tgagaaagac ggcttgttct gcgaagcgat ctttggacct acccgcgatt ggcagtgtta
     3121 ttgcggtaag tacaagaacc cccgttacaa aggtattact tgtgaaaagt gtggtgttga
     3181 agtaacccgt tcttctgtgc gccgtgagcg catgggtcat attgggttgg cttctccggt
     3241 tgcgcacatt tggtataccc gccgcattcc atctcagatg ggtatgttgc tggatatttc
     3301 ccgccgcaat ctggaccgtg tgctttactt tgctcaatat atcgttacct acgtggacga
     3361 agaagcccgc aagaaagccc tccagcgcct tgaagatgag atcaccgttt ctgaacgcga
     3421 acaggcttca gaaatcaata atcgcatcat cgagatcaaa caaaaacgtg atgccagcat
     3481 tagtgaactg aagcaaaagc aagcgaccct tgaacagggg tacgatgaag gtattgctga
     3541 acaacttgac ccggtcatca aagaaggtca gaaactcgaa cgcctccttc aggaccagat
     3601 gggtgaagct gcgaaaaagg ctattgtctt cgaacagacc ggtgaaaaga tcatcgacgc
     3661 tggggacaag gttgcaagca agcacattac cttgatccag aagacagttc agaagaaact
     3721 tgaatcgctt gagaacgagt tgaaggataa gcgtcaggct gagatcgacg atctcaagac
     3781 caaggctgct accatcaaag cgcaggctga tactgagatg gaagctttgc gcaaccagtt
     3841 ggaatcgcag acttcggtct cttcgaacaa ttcttcccgc ttgcgtgacg aattgcttga
     3901 actgcgtccg ttcacgttct tgagcgaaat tcgctatcgt gaattaaagc agcgttgggg
     3961 tcaggtcttc cgcgccgaca tgggtgctga agccttctac g
//
LOCUS       DAOQNJ010000209         3978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14712_length_3978_cov_10.607698, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000209 DAOQNJ010000000
VERSION     DAOQNJ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14712_length_3978_cov_10.607698"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLG52_07175"
     CDS             complement(<1..296)
                     /locus_tag="PLG52_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB41265.1"
                     /translation="MKQYQVSPAEKKLVGSHILVAILALAVGSLFGPLQAFEHAGWDF
                     YPYLKPFIKSYYQGLTVHGVLNALVWTTFFIMGFLTFNVVFGLKRSLKNPVLNKV"
     gene            complement(315..815)
                     /locus_tag="PLG52_07180"
     CDS             complement(315..815)
                     /locus_tag="PLG52_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNB41266.1"
                     /translation="MSTEKLHIDPYERNWMIISAILLVLFATAVSVAAFGLGIQVPAP
                     EQRVDPKTVATDPNSPWSNPGLREIAPGKYDAYVLARAVPNWEYLPKEMSIPAGSSVS
                     FYVTSADLQHGFKIQNTNANFMVIPGQVSKLTIKFDKPGTYNFICTEYCGSGHGVMYG
                     AITVTP"
     gene            complement(812..949)
                     /locus_tag="PLG52_07185"
     CDS             complement(812..949)
                     /locus_tag="PLG52_07185"
                     /inference="COORDINATES: protein motif:HMM:NF019724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 2A"
                     /protein_id="HNB41267.1"
                     /translation="MAKKDKEDDEFRPTGTIAVLTIFVITLIAIWATIYMILVGRGGT
                     L"
     gene            complement(1063..1650)
                     /locus_tag="PLG52_07190"
     CDS             complement(1063..1650)
                     /locus_tag="PLG52_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB41268.1"
                     /translation="MKIRFFLVGIATILVLIAGIYFLRPHTFHGTVIQSPDPSFNFTL
                     KSADGDVSISDFRGKLVLLYFGYTFCPDICPGTLANVAQALREMGAKADDVQLIMVSL
                     DPERDTPEKLKEYMGHFYPSFVGVTGSKEQLDEITSLYGVFYQKKEGSVATGYLIDHT
                     ATLMVLDREGYLKLVFPFGVTSEEIADDLKYMLRQ"
     gene            complement(1666..2340)
                     /locus_tag="PLG52_07195"
     CDS             complement(1666..2340)
                     /locus_tag="PLG52_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB41269.1"
                     /translation="MNKYALNGLYTLFGALAIAAFAFKVFQPIQVLPRMRLSPAYSFI
                     NQDGETVTSEDLRGQFVLYSFTYTNCPAPCYNMGETMKEVQARLNEVDLGGIPVSFVT
                     ISIDPDRDTPEALKTYGTDLGANFDEWMFVTTTNKALLKTIVGSGFETYYEDKGNGSF
                     ALDPGYVLVDGWGIIRAEYRYSTEVSDADRIIRHMGVLADEVKNSKGSTKLAYEAAHL
                     FLCYAP"
     gene            complement(2337..2843)
                     /locus_tag="PLG52_07200"
     CDS             complement(2337..2843)
                     /locus_tag="PLG52_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNB41270.1"
                     /translation="MFRVNRQTDYAVRVVLALAKKPQGTRLPTAEIGREMLIPPSLLQ
                     RIVAELANGGYIKTQPGRDGGVSLAHLPSEITLLQVVEHFEGNLVISDCILKEGDCPF
                     ETKCPVSCQWKRLNDLLIAEMSRITFEQLVEDGKQIEASQMTTTASNQLKVVSNQKSF
                     SLTDSLSA"
     gene            3099..>3978
                     /gene="lgt"
                     /locus_tag="PLG52_07205"
     CDS             3099..>3978
                     /gene="lgt"
                     /locus_tag="PLG52_07205"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNB41271.1"
                     /translation="MIDPVIFSFKLFTWQVTLTWYGLIVMSGVLVAAWFAEREVRRRG
                     ENGEVLIDAMVWAVVAGIIGARLWYVMNALIGGNRSYIEDPMSIIRPPIAGLHFFGGL
                     LFGAIALIIYLRNNGYDVWLFMDAVAPVALLGQAVGRIGNYINQELYGPPTQLPWGIS
                     IPADHRLAQYADLAKYPVESTRFHPTFAYEMVLNVLAFLFIMWYARRDEEEPKPGTLF
                     SMWLIAAGFIRTFIEFFRPDQPRLGDTFITTSMLVAFLMGVTGVILFLARHGKLQIAA
                     LTDWEEEYFIKKVEKKS"
BASE COUNT          995 a          864 c         1016 g         1103 t
ORIGIN      
        1 actttgttca agacaggatt cttaagtgag cgtttgagac caaaaacaac attgaaggtc
       61 aaaaagccca tgatgaagaa ggtcgtccaa accaaggcgt tcaacacacc gtggacggta
      121 agaccctgat agtacgactt gatgaacggt ttgaggtagg ggtagaaatc ccaaccggca
      181 tgttcaaagg cttgcaaagg accgaacaag ctgccaaccg caagtgcgag gatagcaacc
      241 aaaatgtggg agccgacaag tttcttttcg gcaggggata cttgatattg tttcatagtt
      301 gactctcttt gcacttatgg tgtaacggtg atcgcgccat acatcacgcc gtgaccactg
      361 ccgcaatatt cggtgcagat gaagttgtag gtgccgggct tatcgaactt gatggtcagt
      421 ttagagacct gtccggggat gaccatgaaa ttggcattcg tgttctggat cttgaaccca
      481 tgctgaaggt cagcagaggt cacatagaaa gagacgctgg aaccagcagg aatgctcatt
      541 tccttgggta aatattccca gttgggaact gcacgtgcaa gcacataagc atcatattta
      601 ccgggggcaa tctcgcgcaa gccagggttc gaccaggggc tgttcgggtc ggtggcaacc
      661 gtcttcgggt ccacgcgttg ttcaggtgca gggacctgaa tgcccaaacc aaaggctgct
      721 acactcacgg cagttgcgaa caatacgagc aggatggcgc taataatcat ccaattgcgt
      781 tcgtatgggt caatatgcaa tttctctgtg ctcatagtgt gcctccacgt ccgacaagga
      841 tcatatagat cgttgcccag attgcgatga gtgtgatcac gaatatcgtc aagactgcaa
      901 tggtgccagt cgggcgaaac tcatcgtctt ctttatcttt cttcgccatg ttacattctc
      961 ctcttgaaaa aatgaaatta ggatgactac cgcttcacgc ggcgccgcaa gctgcggtgg
     1021 tggataatgg gcagttctct acgctgtagc aggtccacct ccttattgcc gtagcatgta
     1081 cttcagatca tcggcaatct cttcagaagt gacgccgaat ggaaatacca acttcaaata
     1141 tccttcccga tccaatacca ttaaagtagc tgtatggtcg atcaaatacc cggtagcaac
     1201 actgccttcc ttcttttgat agaagacccc atacagtgag gtaatttcgt ctagttgttc
     1261 tttgctacct gtaacgccca caaacgaagg ataaaaatgc cccatgtatt cctttaactt
     1321 ttcaggggta tcccgttctg ggtcaagcga gaccatgatg agctgaacat catcggcttt
     1381 tgcccccatt tctcgcaaag cctgagccac atttgcaagc gtgccggggc aaatatctgg
     1441 gcagaaggtg taaccgaaat aaagcaatac cagtttgccg cggaagtcgc ttatggaaac
     1501 atcgccgtct gcgcttttaa gggtgaaatt aaatgaaggg tcaggggact gaatgaccgt
     1561 tccatgaaat gtatgtggac gtaaaaaata tattcctgct atcaaaacaa gaattgtcgc
     1621 aataccaaca agaaaaaatc ggatcttcat aataacgatg gacctttatg gcgcataaca
     1681 aaggaagagg tgagccgctt catatgctag tttggtagaa cctttgctgt tcttgacttc
     1741 atctgccaga acccccatgt ggcggatgat acggtcagcg tctgagactt cagtggagta
     1801 gcgatattca gcacggatga tgccccagcc atctacaaga acgtaaccgg ggtcgagggc
     1861 aaaactacca tttcctttgt cttcgtagta tgtttcaaaa cctgagccaa caatggtctt
     1921 gagtaaagcc ttgtttgtgg tggtgacgaa catccattcg tcaaagtttg caccgaggtc
     1981 tgtgccgtaa gttttcaacg cttcgggcgt atctcggtct gggtcgattg agatggtgac
     2041 aaatgaaacc ggaataccgc ccaaatccac ttcgttgagg cgggcttgta cttctttcat
     2101 ggtttcgccc atgttgtagc aaggtgctgg gcagttggtg tatgtgaagg agtagaggac
     2161 gaactgtccg cgtaggtctt cgctggtaac cgtttcgcca tcttggttga taaaactata
     2221 agcaggggag aggcgcatgc gggggagtac ttgaatgggc tgaaaaactt taaatgcgaa
     2281 agcagcaatt gcaagagcac caaagagtgt gtacagaccg ttgagagcgt atttgttcat
     2341 gcagagaggc tgtcagtgag agaaaagctt ttttgattag aaacaacttt gagttggttt
     2401 gaggcggttg tcgtcatctg actggcttcg atctgttttc cgtcttctac caattgttcg
     2461 aaggtgatac gagacatttc tgcgatcagt aggtcattta agcgcttcca ttggcagctt
     2521 accgggcatt tcgtctcaaa ggggcaatcg ccttctttta atatgcagtc tgagatgacc
     2581 aaattgcctt caaagtgctc tacgacctgt aaaagggtga tctcgctggg gaggtgagcc
     2641 aagctgacac cgccgtcgcg cccaggctga gttttgatgt agccgccgtt tgccaattca
     2701 gccacaatgc gttggagcaa agaaggcgga ataagcattt cgcgcccgat ttctgcggta
     2761 ggcaaacgcg tcccttgcgg ctttttcgca agagccagca caacgcgaac ggcataatcg
     2821 gtttggcggt ttactcggaa cattaatatc ctttggttta acattgacat ttgatgtcaa
     2881 tgtttactga gattgtaagg atttggggct ttcttttgac gtgttattaa tcacattgtt
     2941 aaatgacaaa cgtctcgtat tcttgtcagc acgtgaagta tttatgacag aatttgttgt
     3001 atttagcaaa aagtaacaat gtgccttata ttgggctata tgtattgtat ggtttgattt
     3061 ggggatataa tcgtcaaaac ttaactcaga ggtaatgaat gatagacccc gttattttca
     3121 gttttaaatt attcacgtgg caagtcacac tgacatggta tggcttgatc gtgatgtctg
     3181 gtgtgttggt tgccgcttgg tttgccgaac gtgaagtgcg ccgccgtgga gagaatggtg
     3241 aagtattgat cgacgcgatg gtgtgggcgg ttgtagctgg cattattggt gcgcgcttat
     3301 ggtatgtcat gaatgcgctg attggtggca atcgttctta tattgaagac cccatgtcca
     3361 ttattcgccc gccaatcgct ggtttacatt tctttggtgg tttgcttttt ggtgcaattg
     3421 ctctcatcat ttatctgcgc aacaacggat atgatgtgtg gctgttcatg gacgcggttg
     3481 cgcccgtggc attgcttggg caggctgtag gcagaattgg taattacatt aatcaggaac
     3541 tgtacggtcc gccaacacaa cttccgtggg ggatttcgat ccctgctgat catcgccttg
     3601 ctcaatatgc agatttggcg aagtacccgg tagagtcaac gcgctttcat ccgacctttg
     3661 cttatgaaat ggtcttgaat gttttggctt tccttttcat catgtggtat gcccgccgtg
     3721 atgaggaaga gcccaagcct ggcactctct ttagcatgtg gttgatcgct gctggattca
     3781 ttcgtacgtt tattgaattt ttccgcccag atcaaccgcg ccttggcgat acttttatta
     3841 ccacctccat gttggttgcg ttcctcatgg gcgtaactgg tgtgattttg ttcctcgctc
     3901 gccatggcaa acttcaaatt gcagcattga cagattggga agaagagtat ttcatcaaga
     3961 aagtggaaaa gaagtctg
//
LOCUS       DAOQNJ010000210         3969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14769_length_3969_cov_8.255749, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000210 DAOQNJ010000000
VERSION     DAOQNJ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14769_length_3969_cov_8.255749"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PLG52_07210"
     CDS             complement(<1..677)
                     /locus_tag="PLG52_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNB41272.1"
                     /translation="MKLGIVTDSTADLPSYLIEQHEIQVVPTVLILEGKEYKDGIGIS
                     REEFYNRLPTLRTPATTAAPSIGDFLTPYQSLLNSGCDHILSIHVSGKLSGVVNIARR
                     AAEEFPGKVTCVDSGSLSMGIGFQVLAAAESADLGVKVALEALASTERRTKVFAALDT
                     MEYLKRSGRVPAVVANIGGLLSIKPVVELRNGEVKPMGINRTTSQADAFILGKLLEVG
                     EMERLAI"
     gene            821..1150
                     /locus_tag="PLG52_07215"
     CDS             821..1150
                     /locus_tag="PLG52_07215"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNB41273.1"
                     /translation="MANTESLENVVLELRRGVIVLAALSQLGSPEYGYSLLKKLADLG
                     LEVDQGTLYPLLRRLETQGLLESVWKLEEARPRRYYVISAEGKKLLPKMKKEWAGIVS
                     VMDKMLS"
     gene            1166..2185
                     /locus_tag="PLG52_07220"
     CDS             1166..2185
                     /locus_tag="PLG52_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41274.1"
                     /translation="MNLIDRYVSEVGKHLPLLNGREDIEKELKSTLEDMLEDRAQSTG
                     RTRDEALEIEVLKEYGSPQKVASTYNPHPYLIGPKLFPFFLFVLKIVLTVVVSVMLVL
                     AGVQAVTDTPFMGADFVKIIGQGLGNALSTAIAAFGNVVLVFVIIERVLPDKEIGGFN
                     DEKDWDPASLTKEPDPDSVSRFELITEIVFTFVGLAILNIYPQWLGMFFVTGEETVFI
                     SLFSEKFFQFVPWINLTLVAQILLDVYLLRNALWNRLSRLFKVFIEVASLSLTVIILR
                     TPGIIGFNAESLLNGPFPPEQAKVFVTIATYTFPIVMAVVIIIQSIELIKSVYKLFKS
                     SRQSS"
     gene            complement(2242..3909)
                     /locus_tag="PLG52_07225"
     CDS             complement(2242..3909)
                     /locus_tag="PLG52_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNB41275.1"
                     /translation="MAIDTARVEKFRKRTIDGQAFKRLVEAGMTWLRTNQQVVNSLNV
                     FPVPDGDTGTNMSLTLQAAWNEVKDKGTKHLGEMAAAFSKGALMGARGNSGVITSQIL
                     RGFSRGVHEVETLDVETFIKALAEARDTAYKGVVKPVEGTILTVIKETANAAEAARGS
                     VKDAIELLELAVKAADEAVKKTPEMLPILKQAGVVDSGGKGLFFFMEGMLRHIYGESL
                     ETPTMQVHALSAVGLEDALEVVEEGQDYEVVVDFVPNGELDLHGFYAKLEEMGTSIQV
                     GEGDGMYRMHIHVPTENRYVPIDYIMGIGTVTKVAIENLMAQMDDIQKSKSSQIQFST
                     VEPGNIAVVVVSPGTGISRIFASLGVAAVVAGGQTMNPSTQDILSSFENMPTDKVIIL
                     PNNKNIVMAANQAKDVTVKSVQVVPTRTVPQGLAAMLSFDPSGDVEAVAAKMNKAMGN
                     VKTGEITVATRSVEIDGVTVKDGQVIALLDGKLVASSESVEQGVFDLLEKAEANEHEI
                     VTLFYGADLPHAEANRVADSIREKYSNLEVEVQEGGQSHYQFILSIE"
BASE COUNT         1004 a         1034 c          889 g         1042 t
ORIGIN      
        1 aaaatcgcta aacgctccat ctcccccact tccaacagct ttcccaatat aaatgcatct
       61 gcctgactgg ttgtgcggtt aatccccatt ggtttaactt ccccattcct caactcgacc
      121 acaggcttaa tggagagtaa accgccaata ttggcaacca ccgccgggac acgtccactg
      181 cgtttgagat attccattgt atctaacgcg gcgaaaacct tcgttctgcg ttctgtcgat
      241 gcgagtgctt ccaacgcaac tttcacacca aggtccgcgg attcggcggc ggcgagcacc
      301 tgaaagccaa tgcccatcga aagcgagcca ctgtccacac aggtgacttt gccggggaat
      361 tcttcggcgg cgcggcgagc aatatttacc acacccgata atttgcctga aacatggatc
      421 gacaggatat ggtcacagcc ggagttgagc agagactgat acggcgtaag aaaatctcca
      481 atggacggcg cggcagttgt cgccggggtg cgaagcgtag gcaggcggtt ataaaactct
      541 tcgcgcgaga tgccgatccc atccttatat tcttttcctt ccaaaataag aactgtaggc
      601 accacctgga tctcatgctg ttcgattaga tacgatggca ggtctgcggt tgagtcagtg
      661 acaattccta gtttcatagg gcaaagtgta gcatatttgg atataccgtt gttcactatg
      721 aaattgcgtc acgagcccct aaaagtggcg ttaaggtcac ctgatacttt aatcttgaaa
      781 tactgtatat tacacagtat aatgtcgtaa aggatgagat atggcaaaca cagaatctct
      841 cgaaaatgtt gttttggagt taaggcgcgg tgtgatcgta ttggcggcgc ttagtcaatt
      901 aggctcgcca gagtacggat attcactgct gaagaaactc gcagacctcg gcttggaagt
      961 ggatcagggt acgttgtatc ccctcctccg ccgcttggaa acacagggtc tgcttgagtc
     1021 agtttggaag ttggaagaag cgcgtccgcg tcgttattat gtgatcagtg cagaaggcaa
     1081 aaaacttctg ccgaagatga aaaaagaatg ggctggcatt gtctctgtca tggacaagat
     1141 gctctcataa agaaagggaa gaatcatgaa tcttattgac cgttatgttt ctgaagttgg
     1201 caagcatttg ccgttattga atgggcgtga agacattgaa aaagagttaa agtccacgct
     1261 ggaagatatg ttggaagacc gcgctcaaag taccgggcgc actcgtgatg aagcactcga
     1321 aatcgaagtc ttgaaagaat acggctcgcc gcaaaaagtt gcatccacct ataacccaca
     1381 tccatatttg atcggaccaa aactgtttcc gttctttctc ttcgtattga agatcgtcct
     1441 tacagtggtg gtcagtgtga tgctggtgtt ggcaggcgtt caagctgtga ccgatacgcc
     1501 tttcatgggc gccgattttg tgaaaatcat cgggcaggga ttgggcaacg ccctctctac
     1561 agcaatcgct gcgtttggaa atgtagtttt ggtcttcgta attattgaac gcgtcttgcc
     1621 ggataaagaa attggtggct tcaatgacga aaaggattgg gaccccgcct cactcaccaa
     1681 ggagcctgat cctgattctg tgagccgttt cgagctgatt acggaaatcg tcttcacctt
     1741 tgtcgggctt gcaatcttga atatctaccc gcaatggctc ggtatgttct tcgttaccgg
     1801 agaagagacg gtctttatct ccctgttctc agagaaattc ttccaattcg taccgtggat
     1861 caaccttaca ttggtcgctc aaattttgct ggatgtttac ctgttgcgaa atgccctttg
     1921 gaaccgctta tctcggttgt tcaaagtgtt tattgaagtt gccagcctct cgctgactgt
     1981 tatcatccta cgcacaccgg ggatcatcgg gttcaatgct gaatctcttt tgaacggacc
     2041 tttcccacct gagcaggcaa aagtctttgt aacgatcgca acctacacct ttcccattgt
     2101 gatggcagtc gttatcatca ttcaaagtat tgaattgatc aaatctgtat acaagttatt
     2161 caaatcgagc cgtcaaagca gttaaataaa aaagaggctg tcacataagt gacagcctct
     2221 ttgcttaact tggtttccca attattcaat cgaaagaatg aactggtagt gagactgacc
     2281 accctcctgc acctcaactt cgaggttgga gtatttctcg cggatagagt ctgcaacacg
     2341 attggcttct gcatggggta aatcggcacc atagaacaaa gtgacaatct catgctcatt
     2401 ggcttctgcc ttctcaagca agtcaaacac accttgctca acagactcag aagaggcaac
     2461 caatttgccg tctaagagcg caatcacctg accatctttc acggtcacac catcaatttc
     2521 cacagaacga gtcgcaaccg tgatttcgcc ggttttgacg ttgcccatcg ccttattcat
     2581 cttcgccgca accgcttcca catcaccgct ggggtcaaaa gaaagcatcg ccgcaagtcc
     2641 ctgcggaacc gtgcgggtcg gaaccacctg cacgctctta accgtcacat ccttggcttg
     2701 gtttgccgcc atcacaatat tcttattgtt cggcaaaata ataactttat ctgttggcat
     2761 attctcaaac gagctgagaa tatcctgcgt agagggattc atcgtctgcc cgccggcaac
     2821 cactgctgca acacccaaac ttgcaaagat acggctaata cctgtgcccg gcgaaacaac
     2881 caccaccgca atattgcctg gctccaccgt gctaaattga atctgtgaag atttgctctt
     2941 ttgaatatca tccatttgag ccatcaagtt ttcaatggca actttggtca cagtgccaat
     3001 gcccatgatg taatcaattg gcacatagcg attttcagtc ggcacgtgaa tgtgcatacg
     3061 atacattcca tcaccctcac ccacttgaat ggaggtgccc atctcttcaa gcttggcata
     3121 aaaaccatgc aagtccaatt caccgtttgg tacgaagtcc accaccactt cgtaatcttg
     3181 tccctcttcg actacttcga gcgcatcttc caaaccaacc gcagaaagcg catgcacctg
     3241 catggtgggc gtctctaaag attcaccata aatatggcgc aacatccctt ccatgaagaa
     3301 gaacaagcct ttaccacccg agtccacaac acccgcttgc ttgaggatgg gaagcatttc
     3361 aggcgttttc ttgaccgctt catctgcggc tttgaccgca agctcgagca attcaatcgc
     3421 atctttaacg gacccgcgag ctgcttctgc cgcatttgcc gtttctttaa tgacggtgag
     3481 aatcgtccct tccaccggct tgacgacccc tttatacgcc gtatcacgag cttctgccaa
     3541 agccttgata aaggtttcaa catctaaagt ctcaacttcg tgtacgccgc gtgaaaatcc
     3601 gcgtaggatc tggctggtga tgacgcccga attgccgcgc gcgcccatca acgcaccctt
     3661 tgaaaatgca gcggccatct cccctaaatg tttcgtgcct ttatccttaa cttcattcca
     3721 agccgcctgc aacgtgaggc tcatattcgt gcctgtatcc ccatcgggaa cgggaaacac
     3781 attcaatgag ttgaccactt gttgattggt acgcaaccag gtcatcccgg cttctacgag
     3841 gcgcttgaaa gcctgtccat caatggtacg tttgcggaat ttctcaactc gcgcggtatc
     3901 gatcgccata tatcgctttt ctcctagtcc gtattgctaa tgcgcaatcc cgctacatgc
     3961 acattgacc
//
LOCUS       DAOQNJ010000211         3939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_14972_length_3939_cov_9.045314, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000211 DAOQNJ010000000
VERSION     DAOQNJ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14972_length_3939_cov_9.045314"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLG52_07230"
     CDS             <1..840
                     /locus_tag="PLG52_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB41276.1"
                     /translation="DTFQPRKLNTPLDKLTRKQAGRRSLTKTERKHGRYIMARPAGDN
                     LTDIAFDATIRAAAPFQKQRTEERKKLAFSIKRSDLQRKIRVKRVSNLVLFLVDASWS
                     MAVAERMNATKGAILSLLTDAYQRRDRVGLIVFQKDRATLVLSPTNSVQLAQRALMDI
                     PVGGKTPLSAGLFMAHDVISHEKHIHPDVEPLLIVLTDGAGNVSLGTLPPQEEGFKFA
                     EMIAMEKIRSVVINMEHAAFDQGLAQQLANHLEAPCFSLNELKAETLYNAVVDEMSKP
                     KKK"
     gene            complement(875..2290)
                     /locus_tag="PLG52_07235"
     CDS             complement(875..2290)
                     /locus_tag="PLG52_07235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB41277.1"
                     /translation="MFFSTTPRPESLRAISDVKLKPFWLDDPSKPEQAPSLTTQIQTD
                     LAIIGGGFTGLWTALQAKQADPHRDVVLLEAGEIAIGASGRNGGFCAASLTHGIENGL
                     SRWKDDLPTLIRLGNENLDDIETTIKQFNIDCDFIRSGEVSVAVEDYQIVDLQEEVQQ
                     AKRFGAKAQFFNQEEIRSRINSPVFLAGAIEKSNAMLNPARLAWGLKKACLQLGVRFF
                     EGTPVTGLEEKRESLILKTPQGQVEARKVAMATNAFPPLLKYLRLYVVPVYDYVLMTE
                     PLSKAQHDAIGWYGREGLSDNANQFHYSQMTSDGRILWGGYDAIYHRNNAVAPQLENR
                     PASFGHLAEHFFQVFPQLEGLRFTHAWGGVIDTSSRYTAFWGQAYNNKLAYVMGYTGL
                     GVGASRFGARVMLDLLNGERTERTELQMVKSKPFPFPPEPFRSPIINFTRWSLDQADR
                     NQGKRNLWLKTLDALGLGFDS"
     gene            complement(2407..3222)
                     /locus_tag="PLG52_07240"
     CDS             complement(2407..3222)
                     /locus_tag="PLG52_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNB41278.1"
                     /translation="MLSQMKNYPPLSATDVRVLMQRKEEGYERKLLVFQTPFGYRRMA
                     ELFVPEEQGTHPLILFLHWYEPEARDSNRSQFVEEAIEMAKAGAICLTVETLWSDPDF
                     FLKRTQADDMQNSMEEVVNERRFIDFLASQPNADLTRFALVGHDFGGMYGILAGSLDQ
                     HPTHYVVMAATPRFPDWYLYLPKLEGAAREAFLSEMSAIDPITHIPNLASVPVFFQFG
                     DNDPHVPLERADEFFAAAKEPKEKKVYQSGHGLNDESTKDRKAWLKEKLQLGS"
     gene            3425..>3939
                     /locus_tag="PLG52_07245"
     CDS             3425..>3939
                     /locus_tag="PLG52_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41279.1"
                     /translation="MSNYRQNNPLGDHLKRLWNNLVYNRQYSRGAAWGAMILGALMAF
                     EVFNFSTTQYALHDMLGDLTFAGLRWSTILAIAFCGIDFAGIARIFTPEQGRDEPAEV
                     YYLFGAWLLAAGFNATLTWWGVSVAIAQNGNIQGTAAVSSETITKIVPIFVAAMVWLV
                     RLLIIGTFSLAG"
BASE COUNT          962 a         1041 c          947 g          989 t
ORIGIN      
        1 gataccttcc agccgcgcaa gctcaatact cctttagata aacttactcg caagcaggcg
       61 ggtcgccgtt cgctcaccaa gaccgaacgc aaacatggtc gctacatcat ggcccgccct
      121 gctggcgata acttaacgga tatcgcgttc gatgcgacca tccgcgctgc agcccccttt
      181 caaaaacaac gaactgaaga acgcaaaaag ctcgctttct cgatcaagcg cagcgacttg
      241 caacgcaaga tccgcgtgaa gcgggtttca aatcttgtct tattcctcgt agatgcttcc
      301 tggtctatgg cggtggctga acgtatgaac gcgaccaaag gcgccatcct ttcgcttctc
      361 accgatgcct atcagcgccg tgatcgcgtg ggtctcatcg tcttccagaa agaccgcgcc
      421 acactggttc tctcccccac caactccgtt caactcgctc aacgcgccct gatggatatc
      481 cctgtcggcg gcaagactcc gctttcagca ggcttgttca tggcacatga tgttatcagc
      541 catgagaagc atatccaccc ggatgtggag ccgttgctga ttgtgttgac cgatggggct
      601 ggcaatgtct ctctcggcac cttaccgcca caagaagaag gcttcaaatt cgctgaaatg
      661 atcgccatgg aaaaaatccg ctcggtggtg atcaatatgg aacacgccgc cttcgatcag
      721 ggacttgccc aacaattggc aaaccatctc gaagcgccct gtttctcgct caatgagcta
      781 aaagccgaaa cgctctacaa cgcggtggtc gacgaaatgt ccaaaccgaa aaagaagtaa
      841 aaaagaggca gtcactagtg actgcctctt tttattacga gtcaaacccc aaccccaatg
      901 catccaaggt tttcaaccaa agatttctct tgccttggtt tcgatctgcc tgatccaatg
      961 accaacgcgt aaagttgata atcggagagc ggaacggttc tggcgggaat gggaagggtt
     1021 tgctctttac catttgcaat tcggttcgtt cagtgcgttc gccattcaac agatctagca
     1081 ttacacgcgc accaaaacga gaagcaccca ccccaagccc ggtgtagccc atgacataag
     1141 ccagtttgtt gttataggct tgcccccaaa atgctgtata ccgagaactg gtatcgatca
     1201 cgccacccca agcatgcgta aagcggagtc cttccagctg aggaaagact tggaagaagt
     1261 gctccgctaa atgtccgaaa gaggcggggc gattttcaag ctgcggcgct actgcgttat
     1321 tgcgatgata aatagcatcg tatccgcccc acagaatccg cccatcggat gtcatttgag
     1381 aataatgaaa ttgattggcg ttatcgctca aaccttcgcg accataccag ccgatggcat
     1441 catgctgcgc ttttgaaagc ggttcggtca tcaagacata atcataaacc ggcaccacat
     1501 acaacctaag atatttaagc aaaggtggga aagcattggt tgccatggct actttacgcg
     1561 cttctacttg accttgtgga gttttgagga tgagcgactc acgcttctct tccaagccgg
     1621 tcactggagt accctcaaaa aagcggacac caagctggag gcacgccttc ttcaaacccc
     1681 acgccaagcg tgctgggttg agcatggcat tagacttctc gattgcgccc gcaaggaata
     1741 caggagaatt gatgcgtgaa cgaatttctt cttgattgaa aaattgagcc ttagcaccga
     1801 agcgtttggc ttgttgcact tcttcttgca ggtcaacaat ttgatagtct tcgaccgcaa
     1861 cgctcacctc ccccgagcgg atgaaatcac agtcaatatt aaattgcttg attgtggttt
     1921 cgatgtcatc caaattctca ttgccaagcc gaatcaaggt tggaaggtcg tccttccaac
     1981 gactcaatcc gttttcgatg ccatgtgtaa gagaagcggc acaaaagccg ccgtttcgtc
     2041 cgctggcacc aatggcaatt tcgcccgctt caagcagaac gacatcccga tgcgggtcgg
     2101 cttgtttggc ttgcagcgcc gtccatagac cggtgaagcc tccgccgata atggcaaggt
     2161 cggtttggat ttgggttgta agagatgggg cttgttctgg tttggacgga tcatccaacc
     2221 agaagggttt caacttcacg tccgatatcg cccgcaaact ctcggggcgc ggagtggttg
     2281 agaaaaacat ggggtctccc gtttcgcgga ccgcccattt caaaaggcag cctcaaagat
     2341 ttgcgtaagt gtaccgggga gatagtgatt gagtcaaata agaaattaag cgtgttttat
     2401 tttttgctaa ctacctaact gtaatttctc ttttaaccat gcctttctat ccttcgtgga
     2461 ctcgtcattc agtccgtgcc cggattgata gaccttcttt tctttcggtt cttttgcggc
     2521 agcaaagaat tcgtctgcgc gttcgagggg aacgtgggga tcgttatcgc caaactggaa
     2581 aaagactggt acagaagcaa gatttggaat atgcgtgatc gggtcaatcg cggacatttc
     2641 ggagagaaaa gcttctcgcg ccgcaccctc taatttgggc agatacaggt accagtctgg
     2701 gaagcgaggc gtggcagcca tgaccacgta atgagtgggg tgttgatcca aactacctgc
     2761 gaggattccg tacatcccgc cgaagtcatg acccactaat gcaaagcgag tcaagtctgc
     2821 gttcggttgt gaggcgagga agtcgataaa gcggcgctcg ttgacgactt cctccatcga
     2881 gttttgcata tcatcggctt gggtgcgctt gaggaaaaaa tctgggtcag accaaagggt
     2941 ttcaaccgtg agacagatcg cgcccgcttt tgccatttca atcgcttctt ccacaaattg
     3001 actacggttc gagtcccgcg cctctggctc gtaccagtgt aaaaacaaaa tcagcggatg
     3061 cgttccctgc tcttctggaa caaaaagctc tgccatgcgg cggtacccaa acggggtttg
     3121 aaagaccagt aattttcttt catagccttc ttcttttctt tgcatcaaca cccgaacatc
     3181 ggttgcagat aatggtgggt aatttttcat ttgagataac atgagcactc ctctggtgtg
     3241 gacaattata gaataaagcc tcgtttcaaa aatcacccta taggagggta tctggcatcg
     3301 attttagatc tgaagcgaaa ttgtgttttc ctcttatttc cctcttgaca cgggaaaaca
     3361 tttgttctaa aatccgttct acttttagaa cactcgttct atcctattcg acaaggaggt
     3421 cgaaatgagt aactatcgtc aaaataatcc actcggtgac catttgaagc gcttgtggaa
     3481 caatcttgta tacaaccgac agtatagccg cggcgctgcc tggggagcca tgatccttgg
     3541 agccttgatg gcgttcgagg tcttcaactt cagcacgacc caatacgctt tgcatgacat
     3601 gctcggcgac ctaacctttg ctggtttgcg ctggtccacc atccttgcca tcgccttctg
     3661 cggtatcgac tttgccggca tcgcccgcat cttcaccccc gaacaagggc gtgacgaacc
     3721 tgccgaagtc tattacctct ttggtgcctg gttgctcgcc gctggcttca acgctaccct
     3781 aacatggtgg ggtgtttcgg ttgccattgc gcaaaacggc aacattcaag gaaccgctgc
     3841 ggtcagctct gaaacaatca ccaaaattgt acccatcttc gtagctgcca tggtttggtt
     3901 ggtgcgcttg ctcatcatcg gcaccttctc tctcgctgg
//
LOCUS       DAOQNJ010000212         3932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_15024_length_3932_cov_7.286046, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000212 DAOQNJ010000000
VERSION     DAOQNJ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15024_length_3932_cov_7.286046"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLG52_07250"
     CDS             <1..983
                     /locus_tag="PLG52_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41280.1"
                     /translation="PVPAGIREYYLPQNYSMPEAFSAAGKPQAYGANIQGVVYKPVLL
                     ASAQIRVLDRKYGVDVEVTKTALVDSLDRRGIVRWEDFTYAGPSLDKLESGPAPSSKY
                     GSIEAPLNDSKLMTALQKDFTDWVFRNSSVEARANVALKVFAGPDVSQADFMKACADT
                     AREARDTEIAKKTSAIEKKIKTLEDKLGREERELREDEAELQNRNIESGANLLELGAS
                     VLGLTRKKSVTTQFTKHRLAQSAKAEVQESQETISKLTKDLNDLIREREQVIREISDK
                     WGRIVSESNEVTINPKKTDIYVNIFGIAWKPYYLIQAGGETTELAAFGVD"
     gene            1087..1647
                     /locus_tag="PLG52_07255"
     CDS             1087..1647
                     /locus_tag="PLG52_07255"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HNB41281.1"
                     /translation="MNLFIILLSLALWGVVHSFLASHLAKDLFGMKRLYRLLYNIFSL
                     VSFAPILYLVATLEDAALYQIPSPWSLVMRGGQLLAALLLLVAVLQTDTLSFVGLRQL
                     FEEEKTGQLVTRGLYKVVRHPLYLFSLLFLWLSPSMSINSLVFNIGVTAYFIIGAYFE
                     ERKLLRDFGEAYADYKRRTSFLIPGL"
     gene            1824..2036
                     /locus_tag="PLG52_07260"
     CDS             1824..2036
                     /locus_tag="PLG52_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNB41282.1"
                     /translation="MSERIVGTVKWFNATKGYGFIGRDGGEDVFVHFSAIQTDGYRKL
                     EAEQKVEFSIEEGPKGLQAANVVPLQ"
     gene            2535..3839
                     /locus_tag="PLG52_07265"
     CDS             2535..3839
                     /locus_tag="PLG52_07265"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB41283.1"
                     /translation="MKYVVIGTGVAGISAVEAIRSVDRAGEIVMLGDDPHGYYSRPGL
                     AYILTGELQDESLFPKKREDYIPLKFKYKKARVIRIDRANKVLHLDDKTPLAYDKLLI
                     ATGASAIPLIVPGANLQGVMKLDHLEDARGILKHAKRGRTAIVVGGGITALELTEGLL
                     ARGVHVHYLLRGNRYWSSVLDEHESRIVEGRLQAEGVTLHFHSEVQEIIGKSGKVRGV
                     RLLDGRIMKCDMLAYAVGIKPRAELAKSADLALDRGILVNEYLQTNDPNIYAAGDVAQ
                     VYDPLTGRSVLDSLWSPAREQGYAAGLNMAGKSQAYVKMVPFNVTRLAGLTTTIIGAV
                     GHGRDEDIVGIARGDSETWRQLPDAIVAQGGFDINRVRLLVGEDYLIGAVVMGDQKLS
                     VPLQRMISEKINISSIRDTLLKKDTKVADVIVDFWSHHVAAV"
BASE COUNT          992 a          874 c          995 g         1071 t
ORIGIN      
        1 cgcccgttcc tgctggtatc cgcgaatatt atttgccgca aaactacagc atgcccgaag
       61 cctttagcgc cgccggaaaa ccccaagctt atggtgccaa tattcaaggc gtggtctata
      121 aaccggtctt gttggcatct gcccagattc gcgtcttaga ccgcaaatac ggtgtggatg
      181 tagaagtgac taagaccgct ctcgtagata gcctcgaccg tcgcggcatt gtccgttggg
      241 aagatttcac ctatgccggt ccttcgctcg acaaactcga aagcggaccc gctccttcct
      301 ccaaatacgg ttccatcgaa gcgcctttga acgactcaaa gttaatgact gctttgcaaa
      361 aagattttac cgattgggtc ttccgaaatt catcggttga ggctcgtgct aatgtcgcgc
      421 tcaaagtatt tgcaggtcct gatgtgagtc aggcagattt catgaaagct tgtgccgaca
      481 ccgctcgtga agcccgcgat actgaaatcg ccaagaagac ctctgccatt gagaagaaga
      541 ttaaaacgtt ggaagacaaa ttaggcagag aagaacgtga actgcgcgaa gacgaagccg
      601 aattgcagaa tcgcaatatc gagtctggag cgaatctttt agagttgggc gctagcgtct
      661 tggggctgac gagaaagaag agcgttacca ctcaatttac caaacaccgt ttggctcaaa
      721 gtgcaaaggc tgaagtacaa gaatcgcagg agaccatttc caagttgacc aaagacttga
      781 atgacctcat ccgcgaacgc gagcaagtaa ttcgcgaaat tagcgacaag tggggcagga
      841 tcgtgagtga gtcaaatgaa gtgaccatca acccgaagaa gaccgatatc tacgtcaaca
      901 tctttgggat tgcttggaag ccttactatc tcatccaagc tggcggtgag acgaccgaac
      961 tggcagcctt tggagtcgat taaatcttaa ttttgtagag tataaaaaag cggatgattt
     1021 gaatatcatc cgctttttct atctattctt tgtaacgaaa ccagctcttg ctgcatctaa
     1081 gaatacatga acctatttat tatcctgctt tcacttgctt tatggggcgt agtacattct
     1141 ttccttgctt cacatctcgc caaagacctg ttcgggatga agcgtcttta ccgcttgctc
     1201 tataatatct tttctttggt gagttttgct cccattctct atctggtagc aacattggaa
     1261 gatgcggctc tctaccaaat cccatccccg tggagtttgg tcatgcgtgg cggacagtta
     1321 cttgcggcgt tgttacttct agtggctgta ttacaaacag atacactttc ctttgttggt
     1381 ttgcgtcaat tatttgaaga agagaaaacc ggtcaacttg tgacgcgcgg tttgtacaaa
     1441 gttgtacggc atccgcttta cttatttagc ttgctgtttt tatggctaag cccgtcgatg
     1501 agtatcaatt cgttggtgtt caacattggt gtgacggcat atttcattat cggtgcttat
     1561 tttgaagagc gtaaactcct gcgtgatttt ggcgaagcct atgcagacta taaacgccgc
     1621 acctcatttc taatccctgg cttgtaaaga acagaccatt gacattagtt ggacatccgt
     1681 caactactgt ctagcggacc atttttcata aaatcttgac accctatttc aggcatgata
     1741 atcttgcccc cgcgtgcccg acccgccttt gaaagcgttt aggtggacgg gcataaattt
     1801 ttatacagca ggagtaaaga aagatgtcag aacgtattgt tggaaccgtt aagtggttca
     1861 atgcgaccaa aggctatggc ttcatcggtc gcgacggcgg cgaggatgtg ttcgttcact
     1921 tcagcgccat tcagacagac ggctatcgca agttggaagc cgaacagaaa gtggagttct
     1981 ccatcgaaga aggtcccaaa ggcttgcaag ctgccaatgt agtgcccctt cagtaagata
     2041 ataaaatatc tttgcctaac agacagggct gtcgtgagac agccctgttt tttttaaatt
     2101 ttacgaaata agacttttaa gagaagtttc atttcttttc aaactcattt catttgtaca
     2161 tctttatgac attcgacact atttggatgc cctcaggcag atttagtata gatatagaaa
     2221 tgccccgtaa ctttttcaaa ttctaagcga ccctgtaaat ggaggatatt cttgtccatt
     2281 cttaatgtat ttaaaaccaa cgccagccgg catacaactg cctcatctgg ttatgtcgtc
     2341 ccagatccgt atcgctgtgt gcaatgcggt atttgtagct ataactgccc catcaacatt
     2401 gatgtccgcg cccatgcgtg gaggggagag cctgtaaagc atagtgaatg tctcacttgt
     2461 ggtgagtgcg tggcgagatg tccgcgcggg gtgttacgct ttgaacggac agacttgttt
     2521 accgaaggtg cctcatgaaa tatgtcgtca ttggtaccgg tgtagcaggc atttcggcag
     2581 ttgaagccat tcgcagcgtg gatagagccg gtgaaatcgt catgcttgga gatgacccgc
     2641 atgggtatta ttcccgcccg gggctggcat acatcctcac aggtgaattg caagatgaat
     2701 ctctttttcc gaagaaacgt gaagattaca tcccgctaaa gtttaagtat aaaaaagcac
     2761 gcgtcatacg aattgaccgt gcgaataagg ttttgcattt ggatgacaaa acgcctcttg
     2821 cgtacgataa gctgctcatt gcaaccggag cttctgccat cccgttgatt gttcccggtg
     2881 cgaacttgca aggcgtgatg aagctcgacc atcttgaaga tgcgcgtgga attttgaagc
     2941 atgccaaacg tgggcggacc gccattgtgg tgggaggcgg tatcacagcg cttgagttaa
     3001 ccgaaggttt actggcgcgc ggagtgcatg tccattatct gttgcgcggc aatcgttact
     3061 ggagcagtgt gctcgatgag cacgagtcgc gcattgttga ggggcgttta caagccgaag
     3121 gagtgactct gcattttcac tctgaagtgc aagagatcat tggcaaatct gggaaagtgc
     3181 gtggagtccg tttattggat ggacgcatca tgaaatgtga catgcttgcc tatgccgtag
     3241 gtatcaaacc tcgcgcagag cttgccaagt ctgcagacct tgctttggat cgcggaattt
     3301 tagtcaacga atatttgcaa accaatgacc caaatattta tgcggcgggc gatgtggcgc
     3361 aagtctacga cccgttgact gggcgttctg tgctggatag tttgtggagc cctgctcgcg
     3421 aacaggggta tgctgctggg ttgaatatgg caggcaagag ccaggcgtat gtgaagatgg
     3481 tgccgttcaa tgtcacgcgc cttgccgggc tgacgacgac cattattggg gcagtggggc
     3541 atgggcgcga cgaagatatt gtgggcattg cccgtggcga tagcgagacg tggcgtcaac
     3601 tgccagatgc gattgtggca caaggcggtt ttgacatcaa ccgtgtccgc ttgttggttg
     3661 gtgaagatta cctaattggc gcagtagtga tgggcgacca aaaactctct gtgcctttgc
     3721 aaagaatgat ctcagaaaaa ataaatattt cttccattcg tgatacgttg ttgaaaaaag
     3781 atacaaaggt tgccgatgtg atcgtggatt tctggtcgca tcatgtggcg gctgtatagc
     3841 ttcattgtta acggtcaaat ttttgcctcg ggagggcaac aaaaatgctt cgtggaaaca
     3901 tagaactttg gctcgctttt cttacttgta ta
//
LOCUS       DAOQNJ010000213         3922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_15093_length_3922_cov_9.943626, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000213 DAOQNJ010000000
VERSION     DAOQNJ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15093_length_3922_cov_9.943626"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLG52_07270"
     CDS             complement(<1..614)
                     /locus_tag="PLG52_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB41284.1"
                     /translation="MSKQDLILLALDASPTLDLMVRAFHSANYEVAVVHDQSGLEKSI
                     QQAIPALMIIGEIFNGEDGFSIATNTLERFPTMPIILYAEKDKPGLAQHIIKAGISGY
                     LYPPLKLDDIVEEVKRTLIRARKLGDWLRREVKRTTASLEQKAQISEAERNKLDAIIS
                     NIQDGVLVLDETHHILLANKTIYEIFSLNGNPILGKYVKDVINSA"
     gene            complement(633..1781)
                     /locus_tag="PLG52_07275"
     CDS             complement(633..1781)
                     /locus_tag="PLG52_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB41285.1"
                     /translation="MATGERILIVESDPDIADLIGRQALKPLGYQVTVVGDAGSAIKF
                     AAQTPPDLILANINLPGLSGKDLLAALNSQNIKSPVIVIAEKGQEPDAIQAFRLGAMD
                     VMFWPLRDAEAVSIVERAMRQTQEARERQKLDRQLKATNDELQRRLRDMTTILTIAKA
                     VTSVNDQRYLFDRILESALQLGEAEMCWLMLREDKGNLFLLRAQRGLPEAWAKKMNQP
                     MDDGISSLVALSGESLFMNGSALQKFKIASLGKSAGVVPIKIKNEVIGLMIVVRKGEK
                     EFVKDSQTMLEAMADYASISLVNARLFRALEQTAENARTGEKARYASLETLRKSIRTE
                     VESAVYPLNLVLTEMPGALNPEQKKALETVKTALQKLSHASENTVTSK"
     gene            1834..2802
                     /locus_tag="PLG52_07280"
     CDS             1834..2802
                     /locus_tag="PLG52_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB41286.1"
                     /translation="MNFSKFNLAVVIPAYRTEKEILAVLQGIPTFIRHIIVVDDASPD
                     SSADLVTAAAKKNRRILLVRHAKNQGVGGAMVTGFKKALELGAEIVIKLDGDGQMDPQ
                     YIPALITPLITGEADYAKGNRFRDFDALRQMPIVRRIGNLGLSFLTKAATGYWNIFDP
                     TNGYFAIRAEMLAQLPLDRIDKGYYFETSMLSRLYLRDAFVQDVTIPARYRNEVSSLS
                     IRRVLFEFPYKLTRTLIKRIILKYFIFDFSMMSIYLLTGIPLLLFGLIFGITKWIQYA
                     ELGIAAPTGTVILPTLSVILAIQILLSAIEIDLNAAPRKAISKALV"
     gene            2804..>3922
                     /locus_tag="PLG52_07285"
     CDS             2804..>3922
                     /locus_tag="PLG52_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41287.1"
                     /translation="MESQPYIPGFKPVDVGPLSRFLPALEDGTISGWLSGQTLAGNWL
                     LDPFGFSPKLVLEAARSGYRVLVTANNPITRFLLEVFANPPPQADFVAALADLGAVKK
                     GDERLEQHLQSLYLTQCEKCEKQIYAQAFLWRKGEDVPYAKLYECPHCKDSGERITTD
                     SDKDIALQIAGTDTLHRTRLFERVVSLKDEDRVYAEEAIEHYLPRPLYVLGTILNRLD
                     SLDLNESRRRALTALLLLAFDAGNTLWGHPAERPRPKLLNTPSQFRENNLWTMLERGI
                     RLFAESGSPVVVEAWPKKIPETGGICIYEGRLKDLAHEVKREIPIAAVVASIPRPNQA
                     FWTLSALWAGWLWDSDAVEPYKVALRRRRYDWAWSATAL"
BASE COUNT          928 a          973 c          949 g         1072 t
ORIGIN      
        1 gcgctgttaa tcacatcttt gacatacttt ccaaggatgg gattcccgtt gagactaaag
       61 atttcgtaga tagttttgtt tgccaacaga atatgatgcg tttcatccaa gactaataca
      121 ccatcttgaa tattggatat gatcgcatct aacttgtttc gttctgcttc tgagatttga
      181 gccttttgtt ccaaggaagc cgttgtgcgc tttacctctc ggcgaagcca atctcccagc
      241 tttcgggcgc ggataagagt gcgctttact tcttcaacaa tatcatccag ttttagaggc
      301 ggatataaat atccactaat gcctgctttg atgatatgtt gagccagccc aggcttatct
      361 ttctctgcat agagaatgat gggcatggtg ggaaaacgtt ctagtgtgtt tgtggcaatt
      421 gaaaagccat cttctccatt gaaaatttcc ccaatgatca tcaatgctgg aatggcttgt
      481 tggattgatt tctctaaacc tgactggtcg tgaaccaccg ctacttcata atttgcggag
      541 tggaaagcac gcaccatcaa atccaacgtg ggcgatgcat ccaatgccag caaaatcagg
      601 tcttgttttg acattacttt ctttgtatat ccttacttgg atgtgactgt attctcagaa
      661 gcatgagaaa gtttttgcag agcggtcttc acggtttcta atgctttctt ttgttctggg
      721 tttagtgcgc cgggcatttc tgtcaacacc aggttgagcg gatagaccgc agattccact
      781 tctgtgcgaa tggatttgcg cagtgtctcc aaagaagcat aacgggcttt ttcaccagtg
      841 cgagcatttt cggcggtttg ttcaagagca cggaacaagc gggcattcac caatgaaatc
      901 gatgcatagt ctgccatggc ttcaagcatg gtttgtgagt ctttcacaaa ttccttttca
      961 cccttgcgca caacgatcat caacccaata acttcattct tgatcttgat agggacaacg
     1021 ccagcagact tgcctaacga tgcaatctta aatttctgaa gcgcggagcc gttcatgaac
     1081 agactttcgc cggaaagcgc caccaatgaa ctaatgccat catccatcgg ttgattcatc
     1141 ttctttgccc aagcttcagg gagccctcgt tgggcgcgca gcaagaagag attgccttta
     1201 tcttcacgca gcatcagcca acacatctcg gcttcgccaa gttgcaaggc actctcaagg
     1261 atgcggtcaa acagatagcg ttggtcgttg actgaggtaa cagccttagc gatggttaga
     1321 atggtcgtca tatcgcgcag gcgacgttgt aattcgtcgt tggtcgcttt taattggcgg
     1381 tcaagttttt ggcgttcacg tgcctcctgc gtctggcgca tggcacgttc tacaatagag
     1441 acggcttctg catcgcgcag aggccagaac atcacgtcca tggcgccaag gcgaaaagct
     1501 tgaatggcgt ctggttcttg tcctttttca gcgataacaa tgacggggga ttttatgttc
     1561 tgagagttca acgcggcgag taggtctttt ccgctcaagc cgggtaaatt gatattggcg
     1621 aggatcaggt ctggcggagt ctgagccgcg aatttgatcg cggagccggc atcgcccacc
     1681 acggttactt ggtatccgag tggtttaagc gcctgtcttc cgatcaaatc ggcaatatct
     1741 gggtctgatt caacaatgag gatgcgttcc ccggtagcca cgtgtaaatc tccttcaaaa
     1801 gattctatca ccttttcgat tacaatcttg aagatgaatt tttcgaaatt caaccttgct
     1861 gttgtgatac ctgcctatcg aaccgaaaaa gaaattctcg cggttctaca gggaattcct
     1921 actttcattc gccacattat tgtagtggat gatgcttcgc ccgattccag cgcggacctg
     1981 gtcactgctg ccgccaaaaa gaatcgacgc atcctcctcg tccgtcacgc aaaaaatcaa
     2041 ggtgtaggtg gcgcgatggt aacaggtttt aagaaagcct tagaacttgg tgccgaaatt
     2101 gtaataaaac tcgatggcga tgggcaaatg gacccacaat acatccctgc gctgattacg
     2161 ccactcatta ccggtgaagc agactatgcc aaaggcaatc gctttcgcga ttttgatgca
     2221 ttaaggcaaa tgccaatcgt acggcgcatc ggcaaccttg gcttgagctt tctcaccaaa
     2281 gctgctacgg ggtattggaa tatttttgac cccaccaatg ggtatttcgc cattcgtgct
     2341 gaaatgctgg cgcaactccc gctcgaccgc atcgataaag gctattactt tgaaacgtct
     2401 atgctctcgc gcttatacct gcgcgatgcc tttgtgcaag atgtgaccat tcctgcacgc
     2461 taccgaaatg aagtttcaag cctttcgatc aggcgtgtgc tgtttgaatt tccatataag
     2521 ctcacccgca ctctcatcaa acgcatcatc ctcaaatact tcatctttga tttttcgatg
     2581 atgtcgatct atttactaac cggcattccg ttgttgcttt tcgggttgat ctttggcatc
     2641 accaaatgga ttcaatatgc cgagttagga atcgctgcgc ccacaggcac ggtcattttg
     2701 ccaacgctct cggtgattct cgccattcaa attttgcttt ctgccatcga aattgacctc
     2761 aacgccgctc cgcgcaaagc catcagcaaa gcgctggtgt gatatggaat ctcaacctta
     2821 cattcccgga ttcaaacccg tagacgttgg tccactctcg cgctttttac ctgcgctgga
     2881 agatggcacc atttcaggct ggctctcggg tcagactcta gccggaaact ggttgctcga
     2941 cccattcgga ttctccccga agcttgtgct cgaagccgcc cgcagcggat accgtgtgct
     3001 cgtcacggca aataatccca tcacacgctt tttgctggaa gtctttgcca acccgccacc
     3061 gcaagcggac tttgtcgctg cgcttgccga tcttggcgcc gtgaaaaaag gggacgaacg
     3121 acttgaacaa catttacaat ccctttacct tacgcaatgt gaaaaatgcg agaaacagat
     3181 ttatgcacag gctttcctct ggcgcaaagg cgaggatgtg ccctatgcaa aactgtatga
     3241 atgcccgcat tgcaaagact ctggtgaacg aataaccaca gactcagata aagacatcgc
     3301 gctgcaaatt gctgggacag atactctaca ccgcacacgt ctcttcgagc gtgtggtttc
     3361 gctcaaagat gaagaccgtg tctatgcgga agaagccatc gagcattatt tgccgcgtcc
     3421 gttgtatgtg ttgggtacca ttttgaatcg gttggatagt ttggatttga acgagtcacg
     3481 tcgccgtgcg ttgaccgcct tgttgttact ggctttcgat gctggcaata cgctttgggg
     3541 gcatccggcg gaacgtccgc gcccgaagtt gctgaacaca ccaagccaat ttcgcgagaa
     3601 caatttgtgg accatgctcg agcgtggcat ccgtctgttt gctgaatcag gctcacccgt
     3661 tgtggttgaa gcgtggccca agaaaattcc agagacgggc ggcatctgca tctatgaagg
     3721 tcgcttgaaa gaccttgccc atgaagtcaa acgtgagatt cctattgcgg cggtggtggc
     3781 ttctattccg agacccaatc aggcgttttg gactctttcg gcattgtggg caggctggtt
     3841 gtgggacagt gacgcggtgg agccatataa agtagcgttg cgacgcaggc ggtatgactg
     3901 ggcatggagt gccacagctt tg
//
LOCUS       DAOQNJ010000214         3909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_15189_length_3909_cov_7.532174, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000214 DAOQNJ010000000
VERSION     DAOQNJ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15189_length_3909_cov_7.532174"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLG52_07290"
     CDS             complement(<1..891)
                     /locus_tag="PLG52_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41288.1"
                     /translation="MRLRLFSIASLLGLLSACSFQSTPAPTPLPPDYLPTVVALTGQA
                     AFATAAALTPSATLPPTETLQPPVEQAIPPTATPTLAAGFTEYAQIRFISPGPMSSLT
                     SPFILQANIAAGESDKIQVDLLGEDGRVLYREIKKLTRNPLGVFQRFEISYEIRAVSE
                     AGYIRISTEDDYGRMQAVNTMPVLLYSVGTAQINPPGNFIYERIAMDGFKEKSNFYAG
                     QVALNGRIWPFSDQAFVVEMISTEGKVLSSRILGINGIDTQPFETVLPYKVTEPTPVR
                     LTFRQDNPLIGISDPVLGKLA"
     gene            complement(946..>1140)
                     /locus_tag="PLG52_07295"
     CDS             complement(946..>1140)
                     /locus_tag="PLG52_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNB41289.1"
                     /translation="IKNLEDQIRKGQFAELREWLREKIHVYGHKYDPQDIVQKVTGSK
                     IDSAAYVRYLTKKYSEVYGL"
     assembly_gap    1141..1240
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1241..1983)
                     /locus_tag="PLG52_07300"
     CDS             complement(<1241..1983)
                     /locus_tag="PLG52_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNB41290.1"
                     /translation="MSEKLAKLKEILGEVSDLGHAGSVLSWDQQVNMPPGGAEARGQQ
                     LATIGKIAQEKFTSDEVGSLLEDLKKEFSDPETDEGAMIRVAARGYDKAKRVPSSFVA
                     EQAMVATKSLEAWVEAKSKSDFSIFRPHLEKNVELVKQYVSFFPPADHPYDTLLDDYE
                     PGMKTSEVREIFGNLRPKQVSLIKAVSEAKQVKDDFLHKSFNEKKVWDFSEKIIAKFG
                     YDFSRGRQDKAPHPFETTFSVNDVRITNR"
     gene            complement(2150..2860)
                     /locus_tag="PLG52_07305"
     CDS             complement(2150..2860)
                     /locus_tag="PLG52_07305"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNB41291.1"
                     /translation="MFNSRTFFALLLFIFAVGCTPVATQTVDSEPQVADATATDEVLL
                     PTATETPLPTATLVPATEEPSATPAPFVHVTAVNGNLYIRRGPSTAYDRIAVLKSATS
                     ATVIGQDMLSKWVQVQIPNSEQTGWVSLLTPYSRIDGDLQGIPAFTFTEWPEPAYIKN
                     CTEHDVIVLPNELYLYSLWTNANYLNYVQVDPGTYQIQDATLPDAPVYDTIDVREGET
                     FYITVNGSGDYHKCPENN"
     gene            complement(2862..>3909)
                     /locus_tag="PLG52_07310"
     CDS             complement(2862..>3909)
                     /locus_tag="PLG52_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41292.1"
                     /translation="GPPGSGPRTSGNNERPLTLGDSNALLDTFAAPSVEMVAPAIQGN
                     ALLTFSSEKTSTSISGVTPEYSQVRNLEIAEGEFINQEHILGRMSVVVLGPETAIDLF
                     GHADGIVGETIRIEGQPFRIIGVLVARGGGAFGSEDNSAYIPFTTAQARLIKRGARDE
                     VDVIFVQATTADAVPSAVEEVSDIIRQRHRTPVGADDFTVFTQQDFLSIFSQITGVLT
                     IFLGGIAGISLLVGGIGIMNIMLVSVTERTKEIGLRKALGARKRDILLQFLTESSLLS
                     LIGGIIGTIFGWVIARIVEQVATATGNTFVPIVGWDAILLSTSFSAMIGLFFGIYPAS
                     RAANLEPVEALRYE"
BASE COUNT         1026 a          902 c          949 g          932 t
ORIGIN      
        1 ggcaagtttt ccaaggacgg ggtcactaat accaataagc ggattgtcct gccgaaaggt
       61 taagcggact ggtgtaggct cggtcacttt ataaggtaag acagtctcaa atggttgggt
      121 atcaataccg ttaatgccca gaatacggga ggataacacc ttcccttctg tggaaatcat
      181 ttcaacaaca aaagcttgat cactaaaggg ccagatgcgt ccattcaatg ctacttgacc
      241 ggcatagaag ttcgattttt ctttgaaccc atccatggcg atacgttcat aaataaaatt
      301 ccctggtggg tttatttgtg ccgtaccaac agagtagagc aaaactggca tagtattgac
      361 ggcttgcatt ctgccgtaat catcctcggt actaatgcgg atgtagcccg cttcagacac
      421 ggctcgaatc tcgtaggaga tctcaaagcg ttggaaaacg cccaaaggat tgcgcgtcaa
      481 cttcttgatc tcgcggtaga gtactcgccc atcttcgcca agcaaatcca cttggatctt
      541 atcgctctca cccgctgcga tattggcttg caaaataaaa ggcgaagtta agctggacat
      601 gggtcccggc gaaataaagc ggatttgggc atattcagta aagcctgctg cgagggttgg
      661 cgtagctgtt ggagggattg cctgttctac aggtggttga agcgtttcag taggcggaag
      721 tgttgccgaa ggtgtaagag cagccgctgt agcaaaggca gcctgtccgg tgagtgcaac
      781 cacggtgggt aaataatcgg gcggtaaagg cgtgggcgca ggcgtggact gaaaggaaca
      841 agcagacaaa agtccgagaa gagaggcgat ggaaaatagt cgaagtcgca tagtggataa
      901 tagaagagac gcccacaggc ggggcgtctc ttgagtcaaa tcaatttaca gcccgtacac
      961 ttcactatat ttctttgtga gataacggac gtatgccgcc gagtcgatct tggagccagt
     1021 gaccttctga acgatatctt gcgggtcata tttgtgacca tagacatgaa tcttctcacg
     1081 tagccactcg cgcaattcag caaattgacc tttgcgaatt tggtcttcca gattcttgat
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tagcgattcg tgatgcgcac
     1261 atcattgacg ctaaacgtag tttcaaacgg atgtggagct ttgtcttgcc tgccacggct
     1321 aaagtcatat ccgaacttgg cgataatctt ttcgctgaaa tcccacactt tcttttcgtt
     1381 gaatgatttg tgcaggaaat catctttaac ctgtttagcc tcactgacgg ctttaataag
     1441 cgaaacctgc ttgggacgca aattaccaaa gatctcacgc acttcagaag tcttcatacc
     1501 gggttcgtaa tcatccaaca aagtatcgta cggatggtct gccggcggga agaacgaaac
     1561 gtattgcttc accaattcaa catttttttc aaggtgcggg cggaagatgg aaaaatcgga
     1621 cttacttttg gcttcgaccc aagcttcaag agattttgtg gcaaccatgg cttgttctgc
     1681 tacaaaagaa gacggcacac gtttggcttt gtcgtatcca cgtgccgcca cgcgaatcat
     1741 agcgccttca tcggtttcag ggtcgctgaa ttctttcttc aagtcttcga gcaaactgcc
     1801 tacttcgtca gacgtgaatt tttcctgcgc gatcttgcca atggttgcaa gctgctgccc
     1861 gcgagcttca gcaccaccgg gaggcatatt cacttgttga tcccaactca atacagaacc
     1921 agcatggcct agatcagaca cttccccaag gatttctttc aactttgcga gtttttcaga
     1981 catcacaatt ctccattgat atttgacgtc aaacgcttcc aaccagaccg gggcttggtc
     2041 ttacatccct gagcatttac cccgctgtat gcggcgtttt cgtcatgatt gtatcacttt
     2101 gccgattaag gcagagaaca cggaatgacc tccgtgttct ctgtatatcc taattatttt
     2161 cagggcattt atgataatcg cccgaaccat tgaccgtaat gtaaaaggtc tcgccttctc
     2221 gcacatcgat cgtatcgtat acgggagcat ctggcagagt cgcatcctga atctgatagg
     2281 taccggggtc cacttggacg tagttcaaat aattggcatt cgtccaaaga gaatacaggt
     2341 acagttcgtt aggcaaaaca attacgtcat gctcggtaca gtttttgatg taagccggct
     2401 cgggccactc ggtaaaggta aaagcaggga ttccctgtaa gtcaccgtca attcgagaat
     2461 aaggcgtcag aagagaaacc cagccagttt gttcagaatt aggaatctga acttgcaccc
     2521 actttgagag catatcttgc ccgatgacgg ttgcagaggt ggcgcttttc agcacggcaa
     2581 tacggtcgta ggcggtactg ggtccgcggc ggatgtagag gttgccatta actgcggtga
     2641 catgcacaaa aggtgcaggc gtggcagaag gctcttcggt agctggaaca agcgtcgccg
     2701 tgggaagagg cgtttctgtc gcagtgggca acaaaacctc atctgtggca gttgcatccg
     2761 caacctgcgg ttcactgtct acggtctgcg ttgccacagg cgtacagcct actgcaaaga
     2821 taaagagcag taaggcaaaa aacgttcgag agttaaacat gttattcgta tcgcaaagct
     2881 tcgacaggtt ccaaattagc agcgcggctg gcagggtaaa taccaaagaa cagaccgatc
     2941 atggcagaga aacttgtcga aagtaaaatt gcgtcccagc caacaatagg cacaaaggta
     3001 ttgccggtag cggttgccac ttgttctaca atgcgtgcaa tgacccaacc gaagatggtg
     3061 ccaataatgc cgccaatgag actcaacaat gatgattcgg tgaggaactg caaaaggatg
     3121 tcgcgcttgc gtgcgccaag ggctttacgc aaaccaatct ctttggttcg ttcagtgacc
     3181 gaaaccagca tgatattcat aataccgata ccgcccacca aaagtgagat acctgcaata
     3241 ccaccaagga agatggtaag cacgccggtg atctggctaa agatgcttag aaagtcttgc
     3301 tgagtaaaga cagtgaagtc atcggcacca acgggtgtgc gatggcgctg ccgaataata
     3361 tcggagactt cttccacggc agacggaacc gcatcggcag tggttgcctg aacaaagatg
     3421 acatccactt catcgcgagc gccacgttta atgaggcgag cttgagcagt agtaaatgga
     3481 atataggcac tgttatcttc actaccaaaa gcaccgccgc cacgcgccac caaaacgcca
     3541 ataatgcgga aaggctgacc ttcaatacga atggtttcac ctacaatacc atcggcatga
     3601 ccgaataggt caatagcggt ttcaggtcca agtacaacaa cggacatgcg tcccaaaatg
     3661 tgttcttgat tgataaattc gccttcggcg atctccaagt tacggacttg tgaatattca
     3721 ggagtaaccc cagaaataga ggtgcttgtt ttttcactag agaacgtgag caaggcattg
     3781 ccttgaatcg ccggtgctac catttctacg gaaggagccg caaaggtatc taacaaggca
     3841 ttggagtcac ccaaggtcaa tggacgctca ttatttccac tggtgcgtgg acctgaccca
     3901 ggaggtcca
//
LOCUS       DAOQNJ010000215         3904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_15228_length_3904_cov_10.121070, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000215 DAOQNJ010000000
VERSION     DAOQNJ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15228_length_3904_cov_10.121070"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3904)
                     /locus_tag="PLG52_07315"
     CDS             complement(<1..>3904)
                     /locus_tag="PLG52_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB41293.1"
                     /translation="RLEMALKGANAGMWDWNVQTGETVFNERWADIVGYSLQELEPIS
                     IQTWADLCHPDDLKLSDALLQKHFARETEFYECEVRMRHKSGSWVWVVDRGKVMEWDA
                     NGKPLRMFGTHLNITERKLSEEEIRKRGEDLLLINTLNDAANRGEPLSNILNTLIQDA
                     RNMFDCQDLSVYLLSPDGKYAELQNLTLSGKRVERIEKLIGRAIPRVQVPIREDGYFK
                     QLLSNEQGTLIEDTASIRQWIEEFVDTPTLPDIVRPLIRKAIPQIQKVIDINTIFAMP
                     LFFSGNVIGLIELSSHTSFDKDDLKRIRTISRQMSAIILRIWAVEKLRESEEKYRTLY
                     KEMPIGVLLVSVSGEVLEANSATLQILGSPSIEATRQINLLTFAPLIEAGLAADFKKT
                     VDSEQINRVERHYLTKWGKSVHLYVTFVPVITPQQELVVQVLIEDITARKDAENNLRA
                     SEEKYRSLIKSLNNSISIVDANGAFLYLNDMAGKELGGPPAELIGKTMHDLFPEPFSS
                     MQLELVRNVIKIDHGYVYEAQSVVNNELRWFRVNFQPLHNENGEVNQVLINANDIHDL
                     KVAQDELQKLNRNLEERVKERTAEVQDLYENAPTGYHSIDADGKFVRVNKTILDWLGF
                     TRDEMIGSPISGFMTSATQKIFSEKFSELVQHGLIKDVEIELIRKDGSILPVLLNATA
                     IRDAAGNFVMSRSTVFDNTERKQAEIELKRNVNFTSALLNAVPTPVFYKDNEGRYLGC
                     NQSFVNMMGKTAEEIYGKLPHEIWESSHADLYRQKDIELFQSNELQTYEGSIRDKHGN
                     LHSAIFVKNVFLDESGKTAGLVGAFIDITERKQAELAIRESEATYRALFENSNDGIFL
                     MAPTGEELRANQHALDLVGYTLDEYLNMGGVSQNPFTLDDDERKDADDKLAALIRGET
                     VPLYERLFTAKDGHKVPVEINLSPVRDANGNLIMVQSVVRDISQRKAAEDALRTINKE
                     LERALRVKDEFLANMSHELRTPLNGILGFSEILLSGEFGSLSERQHKYISSIEGSGKH
                     LLGLINDLLDLSKIEAGKLDVMPENVIISDLCQSSLVFVKQMALKKKIHLALEQDMTY
                     PFIIADQRRLKQILVNLLSNAVKFTPENGKVTLRVAGDPARGCINFSVEDTGIGISPE
                     DLKRLFTPFTQVDSSLTRQHEGTGLGLALVQKLAELHGGGVAVQSEVGKGSTFTVFIP
                     GNYETSLEEMHDHATTKAENKVAAMPTHLNSGKILLAEDIESNIVILGDYLEFQGYTM
                     VYARNGKEALDKAKETLPDLILMDIQMPVMDGLEATRRL"
BASE COUNT          991 a          974 c          798 g         1141 t
ORIGIN      
        1 gcaggcggcg ggtcgcttcg agcccatcca tcactggcat ttgaatatcc atcaaaatca
       61 ggtctggcag agtctctttg gctttatcca gtgcttcctt cccgtttctg gcataaacca
      121 tggtgtagcc ctgaaattca agatagtcac ccaaaatcac aatattcgat tcgatatctt
      181 ctgccaaaag aatcttgcca ctattaaggt gggtaggcat ggcggctacc ttgttttcag
      241 cttttgtagt tgcatggtca tgcatttctt ccaaactggt ttcatagttt ccgggaataa
      301 aaaccgtaaa ggtactacct ttcccaactt cgctttgtac tgcaacaccg cctccatgta
      361 actctgctaa tttctgtacc aatgctaacc ccaacccggt cccttcgtgt tgacgggtaa
      421 ggctgctatc cacttgggta aatggagtga ataagcgctt caggtcttct ggcgaaatgc
      481 caatgccggt atcttccacc gagaagttga tgcagcctct ggctggatcg cctgccacgc
      541 gcaaggtcac tttgccattt tcaggggtga acttcacggc attgctcaac aggttcacaa
      601 gaatctgttt caggcggcgt tggtcggcaa tgatgaaagg ataggtcata tcctgttcga
      661 gcgcaaggtg aattttcttc ttaagcgcca tctgcttcac gaacaccaaa ctcgactggc
      721 acaagtctga aataatcaca ttttcaggca tgacgtctaa tttcccggct tcgatcttgg
      781 aaagatcaag caggtcattg ataagaccca gcaagtgttt accgctgcct tcaatggaag
      841 aaatgtattt atgttggcgc tcactcaaac tgccgaactc tccgctaagg aggatttcgg
      901 aaaagccaag aatgccgttt aagggggtac gtagttcgtg gctcatattc gccaagaact
      961 cgtcttttac ccgcaacgcc cgttccaatt ctttgttgat ggttcgcaaa gcatcttcag
     1021 cggctttgcg ttgcgaaata tcacgcacca cgctttgcac cataatgagg ttgccattgg
     1081 catctcgcac cggcgaaagg ttgatctcta caggcacttt atgcccatct tttgcagtaa
     1141 acaaccgctc atacaatgga accgtttcgc cgcgtattaa tgcagcgagt ttgtcatcgg
     1201 cgtcttttcg ttcatcatcg tccaaggtaa atgggttctg gctgacccct cccatgttca
     1261 gatattcgtc caaggtgtag cctaccaaat ccaatgcatg ctggttggct cgtagctctt
     1321 ctcccgttgg agccatcaag aaaataccgt cattcgaatt ctcaaacaag gcacgataag
     1381 ttgcttcgct ttcgcgaatg gctaactctg cttgctttcg ttcggtaatg tcaataaacg
     1441 cgcctaccaa gccggctgtt tttccgcttt catccaaaaa gacattctta acaaagatcg
     1501 ccgagtgcaa gtttccgtgc ttatcgcgga tgctcccttc ataggtctga agctcattgc
     1561 tttggaataa ttcaatatct ttctggcggt ataaatctgc atgggagctt tcccaaatct
     1621 catgagggag cttgccataa atctcttcag cggttttccc catcatattc acaaacgatt
     1681 ggttgcaacc caaatatctg ccttcgttat ctttatagaa aacaggggta ggcacagcat
     1741 ttagcaacgc gctggtaaag ttgacatttc gtttgagttc gatctctgcc tgtttacgct
     1801 ctgtattatc aaatactgta gaacggctca tcacaaaatt accagcggca tctcgaatgg
     1861 cggtagcgtt caacagaacc ggaaggatgg agccgtcttt tcggatcaat tcaatttcta
     1921 catcttttat caagccatgt tgcaccagct cagaaaattt ctcgctaaat atcttttgcg
     1981 tggcactggt cataaaacca gaaatagggg agcctatcat ttcatctctg gtaaatccca
     2041 accagtctaa aattgtttta ttaacccgca caaacttccc atctgcatca atagaatggt
     2101 agcccgttgg cgcattttca taaaggtcct gcacctctgc ggttctttct tttacacgtt
     2161 cttccaaatt acggttcagt ttttgtagtt cgtcctgcgc tactttaagg tcatgaatgt
     2221 cgttggcatt aatcaagact tgatttacct ctccattttc attatgcaga ggctgaaaat
     2281 taacccggaa ccatcgcaat tcattgttaa ctacactttg cgcttcgtag acatatccgt
     2341 gatcaatctt gataacattg cgcactaatt ccaattgcat ggaagaaaat ggttctggga
     2401 ataaatcgtg cattgttttc ccaattaatt ccgctggagg tccacctaac tccttgcctg
     2461 ccatatcatt caaatataaa aaggctccgt ttgcatctac gatagaaata gaattattta
     2521 agctcttgat caaactgcga tatttttctt cgctggcgcg tagattattc tctgcatctt
     2581 tcctagctgt aatatcttca atcaagactt gaacgaccag ttcctgttgt ggagtgatga
     2641 ccggcacaaa ggtcacatac agatgaaccg actttcccca tttggttaag taatgtcttt
     2701 ctactctatt aatctgttca gaatcaactg ttttcttgaa atctgcagcc agcccagcct
     2761 cgatcagagg cgcaaaggtc aacaggttga tttgccgcgt agcctctatc gatggagaac
     2821 ctaaaatctg caaggtcgcc gagttggctt ccaagacctc tccgctcaca cttaccaaca
     2881 aaacaccaat cggcatctct ttatacaagg tgcgatattt ttcttcgctt tcacggagtt
     2941 tttccactgc ccagatacgc aaaatgatcg ccgacatttg gcggctgatg gtacgaatcc
     3001 gcttcaggtc gtctttatca aaggatgtgt ggctcgaaag ttcaatcaag ccgatgacat
     3061 tccctgagaa gaagagcggc atcgcgaaga tcgtattaat atcgatcact ttttgaattt
     3121 gcggaatcgc ttttctaatc aatggacgca cgatgtctgg caaggtaggt gtatctacaa
     3181 attcttcaat ccactgtcgg atcgaagccg tgtcttcgat cagtgtgcct tgttcgttgg
     3241 ataaaagctg cttgaagtaa ccatcttcgc gaatggggac ttgcacacgg ggtatcgcgc
     3301 gcccaatcaa tttttcgatc cgctcaacgc gtttaccaga aagggtcaga ttctgaagct
     3361 cagcatactt cccatctggg cttagtaaat ataccgacaa gtcctggcaa tcaaacatat
     3421 tgcgtgcatc ttgaataagc gtattcaaga tatttgataa aggttcaccc cggttggcag
     3481 catcgttcaa ggtgttgata agaagcaagt cttcaccgcg cttccgtatc tcttcttcag
     3541 ataatttcct ttcggtaata ttcaggtgcg taccaaacat ccgcaaaggc tttccattgg
     3601 catcccattc catcactttg ccgcggtcta ccacccaaac ccatgagcca cttttatgac
     3661 gcatccgaac ttcacattcg taaaactccg tctctctggc aaaatgtttt tgcagtaatg
     3721 catcagagag tttcaggtca tctggatggc ataaatctgc ccaggtctga atactgattg
     3781 gttcaagttc ttgcaatgaa taacctacga tatctgccca acgctcattg aaaacagtct
     3841 ctcccgtttg cacattccaa tcccacatgc cagcattggc acctttcaat gccatttcga
     3901 gccg
//
LOCUS       DAOQNJ010000216         3901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_15240_length_3901_cov_9.176807, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000216 DAOQNJ010000000
VERSION     DAOQNJ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15240_length_3901_cov_9.176807"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLG52_07320"
     CDS             complement(<1..440)
                     /locus_tag="PLG52_07320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB41294.1"
                     /translation="MNTPFLSIIIPAHNEANRLPRTLEQVFAYLKLQNYSSEVVVVEN
                     GSSDNTLELATHFIKEHSNLVVVQEHQKGKGNAVRRGMLVARGEYRFICDADLSMPIE
                     DLGKFLPPHLNDFDIAIGSREAPGAIRYNEPPYRHIGGRAINLA"
     gene            complement(448..1329)
                     /locus_tag="PLG52_07325"
     CDS             complement(448..1329)
                     /locus_tag="PLG52_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41295.1"
                     /translation="MKVYDRTPLQDAQGNINPIARVQGTLKYGLSWAGELEAQKVVIA
                     QLDRLLDKGFVLIRNFTLPDSDIVIPMILIGPGSISIILATPLKGDFEAKGNEWNSVK
                     NGVSVPASRNLIDLLIKYTRVFQRYLQIHKINIPVQVEPVLIATNPGANIESVRPAVR
                     VVRSDAIKQFATSINQASPVLRAEQVIASADLIIEPHPSSTEKPAEPQIPVERPMSRA
                     QAIFKASEEGDAEKKPKVAAPQPRPRPAQNAQGQAKPPAKKAPAISRRQIFLLVGLGI
                     FECCILFGGIYVLFFLS"
     gene            complement(1339..1641)
                     /locus_tag="PLG52_07330"
     CDS             complement(1339..1641)
                     /locus_tag="PLG52_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41296.1"
                     /translation="MAKKVDGVIEAVRYKNGQIVSVRGYERRGFTFSDRVLLDRKTLL
                     ERLQKGQQFVAGTRQDLLASTFTLSKPILLVKADDREYIATKENVTKDELENVPLF"
     gene            complement(1741..1815)
                     /locus_tag="PLG52_07335"
     tRNA            complement(1741..1815)
                     /locus_tag="PLG52_07335"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1778..1780),aa:Pro,seq:tgg)
     gene            1850..2746
                     /locus_tag="PLG52_07340"
     CDS             1850..2746
                     /locus_tag="PLG52_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB41297.1"
                     /translation="MNTRQAILKAAFSALLLGLSACSTPQTVSPTVTVTVPPPTATAT
                     LTPTPVPPTATPTPLGCLTQPGKVEQGVLTTTDPEQPYMIYLPPCYSELTDQRYPVLY
                     LLHGQTYTMDQWVRLGVPQAMDDMFFSGASVPFIVVFPDDSLWNLSAGWRFGDRLIGS
                     LIPYIDATYRTIPDRDHRAIGGLSRGGGWAAELGLQNPGMFISIGLHSPAIFKGDGPA
                     MQKLVQSIPDESRPRMWIDMGDTDREWESILEFEDVLTYFGYPHEYQRYLGDHSEVYW
                     GAHVHDYLKWYAEVWNETPEGE"
     gene            2797..3774
                     /gene="galE"
                     /locus_tag="PLG52_07345"
     CDS             2797..3774
                     /gene="galE"
                     /locus_tag="PLG52_07345"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNB41298.1"
                     /translation="MNIFVTGGAGYIGSSTAEALIKAGHSVTVYDSLITGHREAVPAG
                     ANFIHASLDDRTALTEALNAQKYDAVMHFAAFIEAGESMKDPGRFFLNNFSNSVQLMD
                     IAVQAGVRKMVFSSTAAVYQSSEEPLTEDSPIGPTNVYGHTKLMTEQALEWYRSIFGM
                     HYAILRYFNACGALPGRGEAHQPESHLIPRVLQIALGQAESATIFGTDYPTPDGTCIR
                     DYIHIADLVSAHILALSALEKRDKMIFNVGSGNGFSVREVIEAARKVTGHAIPAIEQP
                     RRAGDSARLVASPERIKKELGWEPKNTNLQDILSSAWEWHISHPNGYGK"
BASE COUNT          993 a          975 c          905 g         1028 t
ORIGIN      
        1 attgcaaggt taatggcacg tccgccaata tggcgatagg gtggctcgtt ataacgtatg
       61 gcaccagggg cttcacgtga accaatcgca atatcaaagt cattgaggtg gggtggtagg
      121 aatttcccta aatcttcaat tggcatggaa agatcagcat cacaaatgaa gcgatattca
      181 ccacgtgcta caagcattcc gcgccgaaca gcattccctt tgcctttttg atgctcttgg
      241 acaacaacca aattagaatg ttcttttata aagtgggtcg caagctcaag cgtgttatct
      301 gaacttccat tttccacaac taccacttca cttgagtagt tttgcaactt tagatatgca
      361 aaaacctgct cgagagtacg cggcaggcga tttgcttcat tatgggcagg gatgatgata
      421 gataagaatg gcgtgttcaa agcgaggtta gcttaagaaa aacaggacgt agatgccacc
      481 aaatagaata cagcactcaa agatgcccaa ccctactaat aggaatattt gtctacgaga
      541 gattgcaggt gctttcttgg caggaggttt tgcctgccct tgagcatttt gcgccgggcg
      601 tggacgtggc tgtggggcgg caacttttgg ctttttctca gcatcacctt cttcagaagc
      661 cttaaatatc gcttgcgcac gagacatggg gcgctcaacc ggaatttgtg gctcagcagg
      721 tttttctgta cttgaaggat gtggctcaat gataaggtct gcggatgcaa taacctgctc
      781 tgcacgtaag accgggcttg cctgattgat tgaagtagca aattgtttga tcgcatcact
      841 acgtactaca cgaacagcag ggcgcactga ttcgatattg gcgccggggt tggtcgcgat
      901 caaaactggt tccacctgca ctggaatatt gattttatgg atttgaagat aacgctggaa
      961 aacacgcgta tattttatta agagatcgat caaatttcgg cttgccggaa cagatacccc
     1021 attcttaaca ctattccatt cattaccttt tgcctcaaaa tcccctttga gcggagtagc
     1081 caagattatc gaaatggatc ccggaccgat caagatcatt gggatcacaa tatcactatc
     1141 tggcaaagtg aagttacgaa ttaacacgaa ccccttatcc aacaatcgat ctaattgggc
     1201 gatcaccact ttttgggctt ccaattcacc agcccagctc aagccgtatt tcaacgtacc
     1261 ctgaacacga gcaatgggat tgatattccc ttgtgcgtct tgcaatggag ttctgtcgta
     1321 taccttcatt cgatctgcct aaaagagtgg aacgttctcc agctcatcct ttgtaacgtt
     1381 ttctttggtc gcaatatatt cgcgatcatc agctttcacc aacaagatag gcttggaaag
     1441 cgtaaacgta cttgccagta gatcttgccg tgtgccagca acgaactgtt gccctttttg
     1501 aaggcgttcc aacagcgttt tacggtcgag taaaacccgg tctgaaaatg taaagccgcg
     1561 gcgttcatat ccgcgtactg acacgatctg accattctta taacgtacag cttcaatcac
     1621 accatcgact ttttttgcca ttcaaaacct cttcgctgat tttagcacag cttaaattaa
     1681 gagagacacg tatcacacaa ctgtaacgtc tgtgtctccg tgcctccagt tcttggaaag
     1741 tcggggcgac aggattcgaa cctgcgacct cttgctccca aagcaagcgc gctagccgga
     1801 ctgcgccacg ccccgatgac ttctaagcct gccgagtata atgcgaagga tgaatacccg
     1861 tcaagcaatt ctcaaggcag ccttctctgc gcttcttctc ggactttcag cctgctctac
     1921 ccctcaaaca gtttccccca ctgtaacagt gaccgtccca ccgcctacag ccacagctac
     1981 acttacgccg acacctgtcc ctccaaccgc caccccaact ccattagggt gccttactca
     2041 acccggcaaa gtagagcaag gtgtgctaac cacgacagac ccagaacaac cctacatgat
     2101 ctacttaccg ccgtgttact ccgaattgac cgaccaacgt tacccggtcc tttatcttct
     2161 tcacgggcag acctacacca tggatcagtg ggtgaggctt ggtgttccgc aagctatgga
     2221 cgatatgttc ttctctggtg caagcgttcc atttattgtt gttttccctg atgatagctt
     2281 gtggaatcta agtgcaggct ggcgcttcgg agatcggctg atcggttcac tcatcccata
     2341 tattgatgcc acctaccgca ccattccaga ccgtgaccat cgcgccattg gcggactttc
     2401 gcgtggtggc ggttgggcag ccgagcttgg gctacagaat cccggcatgt tcatttcgat
     2461 tggattacat tccccggcaa ttttcaaagg cgatggacct gccatgcaga aactagttca
     2521 aagcattcct gatgaatccc gcccacgtat gtggatcgat atgggtgata cagatcggga
     2581 atgggagagt attcttgaat tcgaagatgt gctgacgtat tttggctatc ctcatgaata
     2641 tcaacgctat cttggtgacc attctgaggt gtactggggg gcacatgtcc atgattattt
     2701 gaaatggtac gccgaagtgt ggaacgaaac cccagaaggc gaataagatt cgtcacaggt
     2761 ataattttga aacgtctatt tgatatagga gcataaatga atatctttgt tacaggcggc
     2821 gcgggctata ttggttcctc tactgccgaa gcacttatta aagcgggaca ttccgtcacc
     2881 gtttatgact cgttgatcac cgggcatcgc gaagctgtcc ctgctggggc gaactttatc
     2941 cacgcatcgt tggatgatcg caccgcactg accgaagcct tgaacgccca aaaatatgat
     3001 gccgtgatgc actttgctgc ttttattgaa gctggcgaaa gcatgaaaga cccaggacgg
     3061 ttcttcctta ataactttag caattcagtg cagttgatgg atattgctgt acaagccggt
     3121 gtgcgtaaga tggtcttttc gtccacagcc gcagtgtatc aatccagcga agagccgttg
     3181 accgaagatt cacccatcgg accgaccaac gtctatggtc ataccaagtt aatgaccgag
     3241 caagcgcttg aatggtatcg ctccattttt ggaatgcatt atgccattct gcgttacttc
     3301 aatgcctgtg gcgctctgcc cgggcgtggt gaagcacatc aaccagagtc acatttgatt
     3361 ccacgcgtgt tgcaaattgc attaggacaa gccgaatctg ctacgatctt tggcaccgat
     3421 taccccacac ccgacggaac ctgtattcgc gattacatcc atattgcaga cttggtttct
     3481 gcgcatatcc ttgccttaag tgcattggaa aaacgtgaca aaatgatctt caatgtaggc
     3541 agcggcaatg gtttctctgt tcgtgaggtc attgaagcgg ctcgcaaagt gacgggtcat
     3601 gcgatccccg ccattgaaca gccgcgccgt gcaggcgact ctgccagatt ggtcgcttcg
     3661 ccggagagaa tcaaaaaaga attaggttgg gaaccgaaga acaccaactt gcaagatatc
     3721 ctttccagcg cttgggaatg gcatatatcg cacccgaatg gatacggaaa ataaaaaatg
     3781 ccatttaaat ttcatcgcat tccggttgga atatgcaatt ctttcctttt gcgaggcgac
     3841 cgtaccattc tgatcgatgg cggagcgatg ggaggtttcc ctgcatttca acgtcaaatc
     3901 a
//
LOCUS       DAOQNJ010000217         3896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_15266_length_3896_cov_9.217912, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000217 DAOQNJ010000000
VERSION     DAOQNJ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15266_length_3896_cov_9.217912"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLG52_07350"
     CDS             complement(<1..478)
                     /locus_tag="PLG52_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB41299.1"
                     /translation="MNNPPRILIVDDEPFNVDYLEQELADFGYELLTAANGEEALEKI
                     RTGSPDLVLLDIMMPIMDGFTVLGQAKADAAIRNIPIIIISANNDLQSVVKGLQKGAE
                     DYLPKPFEPTILYARISASLDRKRLRDQEQVYLQSLENELNIARNIQQEFLPAELPV"
     gene            complement(490..843)
                     /locus_tag="PLG52_07355"
     CDS             complement(490..843)
                     /locus_tag="PLG52_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB41300.1"
                     /translation="MKTILIVEDTDLNIDLLTQLLEDDYKLLIAKDGNQGVNMAISKK
                     PDLILMDISLPILDGYEATRQIRKSLPHTPIIGLSAHAMDGHRQKAQDAGCNDYLTKP
                     VNDSLLFDMLAKYLG"
     gene            complement(856..>3896)
                     /locus_tag="PLG52_07360"
     CDS             complement(856..>3896)
                     /locus_tag="PLG52_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB41301.1"
                     /translation="VAETELDNMIQLIGSQMRDTFNADIAYLALLDQQTGIIQFPYQY
                     GDDIPPTPIGEGMTSRIIRDGKPLIFNRNIEEQSSVLGIQRQGRKARSYLGVPIKAGR
                     ETIGALSVQSTQKEGVFDEDSLRLLSTVAANAGAAIKTARLHAETQRRAREMSTLAEV
                     GRDISSSLEATTVLEGIANHARELLSGDLSALFLPEQNGKIFRAIAAVGEEAEELRND
                     TILLGEGLLGSIALTKKAEIVNDTNNDPRALMITGTESLSDEHMLAIPLLANSELKGL
                     MAVWRHGADNLFTEAELEFMNNLARQAVIAIQNTQLFEQTQHLLNQTEQRAVELAILN
                     SVSESMTRTLDVSTVTHNVGNKIHEIFNAEIVDILLYDAATNIVQLAYSFSDNIHYED
                     EPPWELGEGLTSKIIISKQPLLLNTSQEINDHGAAAYLTAPEDEEEIKSYMGVPIMVG
                     DQVLGVVDVQSYKAGAFNEDNLRILQTLSANMGVAIQNARLFNETQRLFKEAQEARQA
                     AEHANQAKSAFLANMSHELRTPLNAIIGFTRIVRRKSEGLLPDKQTENLDKVLSSSEH
                     LLSLINTVLDIAKIEAGRMDVQAANFNVSSLMDLCANTATPLMKPTVRLVKDVEEDLG
                     MIYSDQDKIKQIVLNLLSNAAKFTAAGKITLDAHRAEDLLVVNVSDTGIGITEEALGR
                     IFEEFQQADTSTTRQYGGTGLGLTISRNLARLLGGDLTATSEHGKGSTFTLTLPIQYG
                     IKPASSLNPDTAPLHRSGTSTKADASKKLILVIDDDPDATYLLRENLGPEEFEIIGAR
                     NGIIGQEKARELKPDAILLDILMPEKDGWQVLHDLKNDPATTNIPIILLTIVDKKALG
                     MRLGAAAYLLKPLNPAEVLSTLQKVIKPKENARIHVLTVDDDPHIAEMLSQQLPESEF
                     RLESALDGEAGLAAIEAEHPDVILLDIMMPKLDGFGVIERLREKPETRELPIIVISAK
                     ELTDDEIARLRESVVLIMRKQGFDGEKLAQEIKHTVQHAN"
BASE COUNT          911 a          932 c          958 g         1095 t
ORIGIN      
        1 acacgggcaa ctcggctgga agaaattcct gctgaatgtt gcgcgcaatg ttcaactcgt
       61 tttcaaggct ttgtaaatag acttgttcct ggtcacgcag gcgtttccta tccaaactgg
      121 cggagatacg cgcatataaa atggtcggtt cgaaaggctt gggtaaataa tcttcggcgc
      181 ctttttgaag ccccttcacc acgctttgca gatcgttgtt cgcagaaata ataatgattg
      241 ggatattacg aatcgccgca tcagctttgg cttgccctaa taccgtaaac ccatccatga
      301 tgggcatcat aatatctagt agtacaaggt ctggagatcc agtacgaatt ttctccagtg
      361 cctcctctcc gtttgcagcg gtaagaagct catacccaaa atcagcaagc tcctgctcta
      421 ggtaatcaac attgaatggt tcatcatcta cgatcaaaat acgcggtgga ttgttcataa
      481 aagaaaggct tatcccaagt atttggcgag catgtcaaac agtaaagaat cattcacggg
      541 ttttgtgagg taatcgttac agcctgcatc ttgtgctttt tgtcggtgcc catccatggc
      601 gtgggctgaa agaccaatga tcggcgtgtg cggaagcgac tttcgtatct gacgtgtagc
      661 ttcataccca tccagaatcg gcagcgaaat atccatcagg atcaaatcag gctttttgct
      721 tattgccatg ttcacaccct gattaccatc tttagcgatt aacaatttgt aatcatcctc
      781 aagtaattgg gtgagtaggt caatgtttag gtctgtatct tcaacgatta atatagtttt
      841 catacactct ccaatttaat ttgcgtgttg cacagtgtgt ttgatttctt gtgccaattt
      901 ttcaccatca aagccttgct tgcgcatgat aagaactaca ctctcgcgca atctcgcaat
      961 ttcatcatcg gtcaactctt tggcgctaat gacgatgata ggcagctcac gcgtctcagg
     1021 cttttcgcgc aagcgttcga tgacgccaaa accatccaac ttgggcatca taatatcaag
     1081 caagatgacg tccggatgct ccgcctcaat cgccgccaaa cctgcttcac catccaaagc
     1141 ggactctagt ctgaattcgg attctggtaa ctgttggctt aacatctcag caatatgcgg
     1201 gtcatcatcc actgtgagaa catgaatacg agcattctct ttaggtttga tcaccttttg
     1261 caacgtggat aacacttctg cggggttgag cggtttgagc aaataggcag cggcgcccaa
     1321 gcgcatgcct agcgcctttt tatccacaat tgtaagcaga atgattggaa tattagtcgt
     1381 tgctgggtcg tttttcaggt cgtgcaaaac ttgccagccg tctttctctg gcatgagaat
     1441 atcgagcaaa atggcatctg gctttagctc gcgggctttt tcctgcccga tgatgccgtt
     1501 gcgagcgccg atgatctcga attcttcggg tccgagattt tcgcgcaaaa gataagtagc
     1561 atcggggtca tcgtcgatca cgagaatgag ctttttggaa gcatcagctt tggttgaggt
     1621 gccagatcga tggaggggag ccgtatcggg attaagtgac gaggcgggtt ttatcccata
     1681 ttgaatgggg agggtgaggg tgaatgttga accttttcca tgttcactgg tggctgttag
     1741 atcgccgccc aagaggcgtg ccagattgcg gctgatggtg agacccaacc cggtgccgcc
     1801 atattggcgg gtggtgctgg tatccgcttg ctggaattct tcgaagattc tgcctagggc
     1861 ttcttcggtg ataccaatac ccgtatcgct gacgttgacg accaataaat cttcagcgcg
     1921 gtgcgcatct aatgtaatct tgcctgccgc agtgaacttg gcagcattgc tcaataagtt
     1981 gagcacgatc tgtttgattt tatcttggtc agaatagatc atgccgagat cttcttctac
     2041 atcttttacc aaacgaaccg taggcttcat gagcggagtt gccgtattgg cacacaggtc
     2101 catcagcgaa ctgacgttga agtttgccgc ctgcacgtcc atgcgcccgg cttcgatctt
     2161 ggcaatatcg agcacggtgt tgatgaggct aagcaaatgc tcagaactgc ttagaacctt
     2221 atcaagattt tcggtttgtt tatctggcaa taagccttcg ctctttcttc tcacaatgcg
     2281 tgtgaagcca atgatggcat tgagcggcgt gcgtaactca tggctcatat tggcaaggaa
     2341 ggcgctcttt gcctggttgg catgttctgc tgcctgacgg gcttcttgag cttctttgaa
     2401 aagacgctga gtttcattga agaggcgggc attttgaatg gcaacgccca tgttagcgga
     2461 gagagtttgc agaatgcgca aattatcttc gttgaaggcg ccggctttat agctttgaac
     2521 atccaccacg cctagcacct gatcgccgac cataatgggc acacccatat acgacttgat
     2581 ttcttcttca tcttcagggg cggtgagata ggctgccgca ccatggtcat tgatctcttg
     2641 cgatgtattc aagagcaaag gctgtttcga gatgatgatc ttggatgtaa gcccttcacc
     2701 caattcccaa ggtggttcat cttcgtagtg gatattgtcg ctaaagctgt atgccaattg
     2761 aacaatattc gtagcggcat cgtagagcag aatatctacg atctcggcgt tgaagatctc
     2821 atggatcttg ttaccgacat tgtgcgtcac ggtgctgaca tccaatgtgc gcgtcatgga
     2881 ttcactgacg ctgttaagaa tagcaagttc cacggcgcgt tgctcggttt gattgagcag
     2941 gtgttgagtt tgctcaaaca actgggtgtt ctgaattgcg atcactgcct gacgcgcaag
     3001 gttgttcatg aattccagtt cagcttcggt gaagagatta tcagctccat gacgccaaac
     3061 tgccatgaga ccttttagct cagaattagc aagcaatgga atcgcaagca tatgctcatc
     3121 tgataaagac tctgttccag tgatcatcaa tgcgcgtggg tcattgttgg tgtcgttaac
     3181 gatctccgct tttttcgtga gcgcaatact gccgagcaac ccttccccaa gtaagatggt
     3241 gtcgtttctg agttcttcgg cttcttcgcc cactgccgca atggcacgga aaatcttccc
     3301 attttgttct ggcaggaaga gcgcactgag gtcaccactt agcaactctc tggcatgatt
     3361 tgcaatgcct tcaagcaccg tagtagcttc gaggctggat gaaatatcgc gcccaacttc
     3421 tgccagcgta gacatttctc ttgcacggcg ttgcgtctca gcatgtaagc gcgcggtttt
     3481 gatggcggca cctgcattgg cagcaacggt tgaaagcaaa cgcagcgagt cttcgtcgaa
     3541 tacgccttcc ttctgcgtgc tttgaacact gagcgcaccg atcgtttcgc gaccggcttt
     3601 aatcggcacg cccaaataag accgcgcttt tcgtccctgg cgttgaatgc caagcaccga
     3661 gctttgctct tcaatgttac ggttgaagat caatggtttc ccatcacgga tgatgcggct
     3721 ggtcatgcct tcgccaattg gggtgggtgg gatgtcatcc ccatattgat aagggaattg
     3781 gataatgcct gtctgttgat cgagcaatgc cagataagca atatcggcat tgaacgtatc
     3841 ccgcatctga ctcccgatga gctggatcat gttatccagt tctgtctctg ccacca
//
LOCUS       DAOQNJ010000218         3885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_15336_length_3885_cov_11.761097, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000218 DAOQNJ010000000
VERSION     DAOQNJ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15336_length_3885_cov_11.761097"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..>1142
                     /locus_tag="PLG52_07365"
     CDS             16..>1142
                     /locus_tag="PLG52_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41302.1"
                     /translation="MLRDVIVDTVAAPIRLAKAMIGKSDRRMNQNYVWALKDISFDLE
                     EGKVLGIVGRNGAGKSTLLKILSRVTEPTTGSVTVRGRVGSLLEVGTGFHPELTGREN
                     IYMNGAILGMKRSEIDAKFDEIVDFSEVTQFIDTPVKRYSSGMYLRLAFAVAAHLEPE
                     ILVVDEVLAVGDAEFQKKCLGKMGDVAQQGRTVLFVSHNMSAILRLTQEAIVLNKGQL
                     IMRGPTQEAVDYYLSSGQSQAGERTWDADEVPAASAPFTPISLKVKERGGKVVDTIRS
                     TETVTVEFEYKLSSPVTGLRVGLYVSTMRGEYVFTSFDTDTPALYEKFDSRDAGRYIS
                     RAELPADIFNEGRYTVGVNASSFGVRRYFMDENALAFNVDIS"
     assembly_gap    1143..1242
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1243..2895
                     /locus_tag="PLG52_07370"
     CDS             <1243..2895
                     /locus_tag="PLG52_07370"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41303.1"
                     /translation="TNYHMPKSNPLREILGDLPFTAEIDWILRSKNRPRKDHYNLTRL
                     LKSLPDAVKVVKPYAEKAPLGKNVLFFSTLHYWIEQSAYLGLVLAGMGHDVTLLTLPY
                     SEWHKEKDKFTQRQRILHTHDALKMLSPLVKHISMLDMQSSSRASHGTLHAHASNGLP
                     EKIQADIKEISLWDVQYTLMREEVDMNDAGDKALYDLRVERNTFAAKAALQYMQANKP
                     DVVLIPNGLILEMGIVFRVARYLGIDAVTYEFNDQREQIWLAQNSSIMQQETDYLVDA
                     RCSLPMTDAMYERVADLENARRGARVWGKSKRLWQYVSSQGADVTRKQVGLDDRPVVM
                     LAANVLGDSLTLGRDIFASSMTEWITKTVQYFAKRTDVQFVIRVHPGEKLVPQAKSMA
                     TVVKEALPELPDHIHVIGALDKVNTYDLIEIANVGLAYTTTVGLETAMNGRPVISCGQ
                     THYRGRGITLDPNTWDEYYATLEKVLGDIPAHQMNEKQIEFAWNYAYRFFFEYPRPFP
                     WRLMNFWDDLAEHPLESVLSPEGMARFKKTFDFLVCEPFTWE"
     gene            3012..3836
                     /locus_tag="PLG52_07375"
     CDS             3012..3836
                     /locus_tag="PLG52_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB41304.1"
                     /translation="MTNETKQKVREFYDEIGWTQEDDGNYQNARYEDLRPVSREYIHK
                     TRLRVMNGLIPTGRFILDAGSGPVQYEEYKTYSAGYEKRVCLDISIQALREARTRIGD
                     HGLFVVGDLANLPFKKESFDGAVSMHAIHHLALPEHPKAYAEIHRVLAPGRTAAIVNG
                     WSDPLISRLAEPFIGLLRKMTGRDAKKKKEWLREDAPAGTFVEKMTPAWLKREIGSKM
                     TIEIKPWRSMSTRILRNFIRPFGGRAFLKFIFWLEGVFPKFFAENGQYPLIVIRKA"
BASE COUNT          900 a          915 c         1019 g          951 t
ORIGIN      
        1 aaattccgct ataacatgct gcgcgatgtg atcgtggaca cggttgccgc gcccatccgt
       61 ttggcgaagg caatgatcgg aaaatctgac cggcgcatga accagaatta tgtttgggcg
      121 ttgaaggata tttcatttga tcttgaagaa ggtaaagtac ttggcattgt gggacgtaac
      181 ggcgcgggca aaagcacctt gctcaagatt ctctctcgcg tcaccgagcc gacgaccggt
      241 tctgtcaccg tgcgcggacg ggttggctcc ctgcttgaag tgggaactgg cttccaccct
      301 gagttgaccg gacgtgagaa catttacatg aacggtgcga tcttgggcat gaagcgttct
      361 gagatcgatg cgaagttcga tgagatcgtg gatttctctg aagtgacaca gttcatcgac
      421 acccctgtga agcgctattc atcgggtatg tatctgcgcc ttgcatttgc tgttgctgca
      481 catctcgaac cggaaatcct cgttgtggat gaagtgttgg ctgtgggtga tgcagaattc
      541 cagaaaaaat gcctgggtaa gatgggtgac gttgctcaac agggacgcac cgttttattc
      601 gttagccata acatgtctgc gattttgaga ttgactcaag aagcgatcgt gctgaataaa
      661 ggtcaactca ttatgcgtgg accgacccaa gaagcggtgg attactacct ctcttcgggg
      721 caatctcaag caggcgaacg cacttgggat gcagatgagg ttccagcggc gagtgcgcct
      781 tttacgccga tcagcctcaa ggtcaaggaa agaggcggga aggtcgtcga taccatccgc
      841 tctacggaaa cggtcacggt tgagttcgag tacaagttat cctcgccggt tactggatta
      901 cgtgttggtt tgtacgtcag caccatgcgt ggagaatatg tctttacgtc gttcgatacc
      961 gatacgcccg cgctgtacga aaaatttgat tcgcgggatg cggggcgtta cattagccgc
     1021 gccgaactgc ccgccgatat tttcaacgaa gggcgttata ccgtgggcgt gaatgcgagt
     1081 tcatttggcg tgcgccgcta ttttatggat gagaacgcgc tggcgttcaa cgtggatatt
     1141 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnactaatta ccacatgcca
     1261 aaatcaaacc cacttcgcga aattttgggc gacctgccct tcactgccga gatcgattgg
     1321 atccttcgct ccaagaatcg tccgcgcaaa gatcattaca acctgacgcg tttactcaag
     1381 tctttgcccg atgcggtgaa ggttgttaag ccttatgctg agaaagcgcc gctggggaaa
     1441 aatgttttgt tcttctctac gcttcattat tggatcgagc aatcggcgta tcttgggttg
     1501 gttttggcag gcatggggca tgatgtcaca ttgttgaccc tgccgtattc cgaatggcat
     1561 aaagaaaaag ataagttcac tcaacgtcaa cgcattcttc atacgcatga tgcattgaaa
     1621 atgttatctc ctttggtgaa gcatatttcg atgctggata tgcaatcctc ttcacgtgct
     1681 tcacacggca cgcttcatgc tcatgcttca aatggattgc ctgaaaaaat ccaagctgat
     1741 atcaaagaga tttctctttg ggatgtgcaa tataccctga tgcgcgaaga agtggatatg
     1801 aacgacgcgg gcgacaaagc cttgtatgac ctgcgcgtgg aacgcaacac cttcgccgcc
     1861 aaagccgcgt tgcaatacat gcaagccaat aagcccgatg tggttttgat cccgaacggg
     1921 ttgatcctgg aaatgggaat tgtcttccgt gtggcgcgtt acctcggcat tgacgcggtg
     1981 acctacgaat tcaacgacca gcgcgaacaa atctggctgg cgcagaactc gtccatcatg
     2041 cagcaggaga cggattatct ggtggatgca cgttgctctc tgcccatgac cgatgccatg
     2101 tatgaacgtg tggctgattt ggagaatgcc cggcgcggtg cgcgagtgtg gggcaagtcc
     2161 aaacgcttgt ggcagtatgt ctcgtctcaa ggtgcggatg tgactcgcaa acaggtcggt
     2221 ttggatgacc gcccagtggt gatgctcgcc gcgaatgtac tcggcgacag cctcacgctt
     2281 gggcgtgaca tcttcgcctc ttccatgacc gagtggatta ccaagaccgt tcaatatttt
     2341 gccaaacgga cggatgtgca gttcgtcatc cgtgttcacc cgggtgaaaa actcgtgccg
     2401 caagccaagt cgatggcgac ggtggtgaaa gaggcgttgc ccgaattgcc agaccatatt
     2461 cacgtcatcg gcgcgttgga caaggtcaac acctatgacc tgatcgaaat tgcgaatgtc
     2521 ggtttagcct acactactac ggttggactt gaaaccgcca tgaacggacg tcccgtcatc
     2581 tcgtgtggac aaactcacta tcgcgggcgc ggtattaccc tcgacccgaa cacttgggat
     2641 gaatattacg ccacgcttga aaaagtgctg ggcgatattc ctgcccatca aatgaacgaa
     2701 aagcaaattg aatttgcctg gaattatgcg taccgatttt tcttcgagta cccgcgtccc
     2761 ttcccgtggc gcttgatgaa tttctgggat gatcttgccg aacatccact cgaaagtgtg
     2821 ttgagccccg aaggcatggc aagatttaag aagacctttg acttcctggt ttgcgaacct
     2881 tttacatggg aatagcatgt cattgcgagg gcgcttttgt tctttgcccg aagcaatccc
     2941 cttatccatt ggagattgtt tcgtcgggaa aagcaccctc ctcgcaatga cataaataat
     3001 tgagagagat aatgaccaac gaaaccaaac aaaaagtccg cgaattttat gatgagatcg
     3061 gctggacgca ggaggatgac ggcaattatc agaatgcccg ttatgaagac ctacgtcctg
     3121 tctcacgcga atacatccac aagacgcgtc tgcgcgtgat gaatggactc atcccaactg
     3181 gtcgcttcat tcttgacgcc gggtctggtc ccgtccagta tgaagaatac aagacctatt
     3241 ctgctggcta cgaaaagcgc gtctgtctgg atatttccat ccaagcctta cgcgaggcgc
     3301 ggactcgtat tggagaccat ggtctattcg tcgttggtga cttggcgaat ttgccgttca
     3361 aaaaagaatc cttcgatggc gcggtttcga tgcacgcgat ccatcacctc gctttgcctg
     3421 aacatcccaa agcctatgcc gagattcacc gcgtactagc accgggtcgt acggctgcca
     3481 ttgtcaatgg ctggagcgat ccacttatta gtcgcctggc agagccgttt attggattgc
     3541 tgcgtaagat gaccggacgc gatgccaaaa agaaaaagga atggttgcgc gaagatgctc
     3601 ctgctggaac ctttgtagag aagatgacgc ctgcctggct caagcgtgag atcgggtcga
     3661 aaatgaccat tgagatcaag ccatggcgca gtatgagcac ccgcatcctg cggaatttca
     3721 ttcgtccctt tggcggacgg gcgttcttga aatttatctt ttggctcgaa ggtgtttttc
     3781 cgaaattctt tgcggaaaac gggcaatatc cgttgatcgt aattcgtaaa gcgtgatgcg
     3841 tgatgcgtga tgcgtgatgc gtgatgcgtg atgcgtgatg cgtga
//
LOCUS       DAOQNJ010000219         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_15800_length_3817_cov_6.359383, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000219 DAOQNJ010000000
VERSION     DAOQNJ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15800_length_3817_cov_6.359383"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PLG52_07380"
     CDS             <1..959
                     /locus_tag="PLG52_07380"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HNB41305.1"
                     /translation="QDPEKEVPVILTTSQMLTTGVDAPTCRNVVLFRTINSMTEFKQI
                     IGRGTRVSEEHGKYWFTILDYVGATQLFYDPAFDGDPVRVTKTKIDEDGNETSVEDSE
                     EGTLQDEQDLTGFENLSGLQQKIGELPRKYYLDNLHVYIAGEQAFELDPDGNVLKTVE
                     FTDYVTQNIRRLNLTAEHLRQVWTQAEQRKEILDQLRARGIDPDHLAHVTHHEEADAL
                     DLLLHVAYNAPLVSRRERAEKLRQKKANFFNTFTPAAREILDTLLEKYADYGIGEFDQ
                     LPRVLQVSPFDQYGTTHDIYRLFGGAEKLLQAVDELQKFLYE"
     gene            981..1715
                     /locus_tag="PLG52_07385"
     CDS             981..1715
                     /locus_tag="PLG52_07385"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNB41306.1"
                     /translation="MKPNNLSEEKIKSTLLKTKVTLEAIVSFVEEFDRGSLSLVLQQN
                     FYERLASFRETLFHLQRNTSDMISSLENKNEEVTQLQSEIQELQRRLHELEAFEKRST
                     QDGGVFISYSHADKDFVNEIVKEFETDKINYWLDDKDLFIGQVIDKAISDGIQKSWIF
                     ITVLSPSSVNSRWVEREFDEASHEEVEGRKIILPVVCNNLPADKVPARIRRKLYVDLS
                     KDFPSGYQKLKKSILHYLEEYKKNQK"
     gene            1737..3311
                     /locus_tag="PLG52_07390"
     CDS             1737..3311
                     /locus_tag="PLG52_07390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNB41307.1"
                     /translation="MPRTKKSTNPAPSAAKAAPPPKSKKRILGEEKQHNTKASLSSLI
                     KSARDIMRKDAGLNGDLDRLPQLSWILFLKCYDDLEMRREEDAKLERKAYKPVIPLPY
                     RWRDWASDEDNGATGEDLVKFVNDGLLPALRGLSGSDERLKVAEVFKETYNRMLSGYL
                     LRDVVNLVDNIHFQSKDEIFTLAHLYESMLKEMRDAAGDSGEFYTPRPVVKLIVDRVK
                     PRLGESVLDPACGTGGFLVESYEYLKPRVKNSKEQEVLQTRTLFGVEKKPLPYLLSTM
                     NLLLHGIETPNIRRDNALKNPLNEIGEKDRFDVIVTNPPFGGEEEAGIQLNFPENTRA
                     SETALLFMQFVMRSLRHGGRCGVVVPNGFLFGDGVAARVKAQMLRDFNLHTIIRLPNG
                     VFAPYTSIPTNLIFFDRTQPTTDIWFYEHVLPEGVKNYTKTRPMQFEEFQPILDWWDD
                     RVENERAWKVRAADVIQTNGGGDVVSVNLDIKNPSRKEDLEHLPPAELAESILAKEKQ
                     IAAIMEEIKSLLAKAQ"
     gene            3311..>3817
                     /locus_tag="PLG52_07395"
     CDS             3311..>3817
                     /locus_tag="PLG52_07395"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNB41308.1"
                     /translation="MSKYPKVKFGDFVEHIVREVELEADKEYPSVGVKWYGEGVHIHE
                     IKSGGSFNASRFEIKANDIIYNDMWARNGSVAIVPSHLDGTVASSHFPTFLPDLTRVL
                     PKYLEFYFKTRTFWDDCENASRGSTGRNQIKKSTFYAIEIPLPLLTEQHRIVAHIESL
                     AARVNEAQR"
BASE COUNT         1032 a          777 c         1078 g          930 t
ORIGIN      
        1 tccaagaccc tgaaaaggaa gtgcctgtta ttttgacgac ttcgcaaatg ctgaccacag
       61 gtgtggatgc gccgacctgc cgtaatgtgg ttttgtttcg cacgatcaat tctatgacgg
      121 agttcaagca aatcataggg cgtgggacgc gggtttcgga agaacatggc aagtactggt
      181 ttaccattct ggactatgtg ggcgcgacgc agttgtttta tgaccctgcg tttgatggcg
      241 acccagtacg ggtgaccaag acaaagatag acgaagacgg taatgaaacc agtgtggaag
      301 atagcgaaga agggacgctt caagatgaac aagacctgac aggttttgaa aacctgtcag
      361 gtctgcaaca gaaaattggc gagctgccgc gcaagtatta tttggacaat ttgcatgtgt
      421 atattgcagg tgaacaagcc tttgagcttg accctgacgg gaatgtacta aaaacggttg
      481 agttcaccga ttatgtgact cagaacattc gccgcttgaa tttgactgcc gagcatttgc
      541 ggcaggtgtg gacgcaggca gagcagcgca aagagatttt ggatcaattg cgcgcccgtg
      601 gaattgaccc tgaccatctg gcgcatgtga cccaccacga agaagcggat gcgctggatt
      661 tgctgttgca tgtagcgtac aacgcaccgt tggtgagtcg ccgcgagcgg gcggagaagt
      721 tacgccagaa gaaagccaat ttcttcaata cgtttacgcc tgcggcacgt gagattttgg
      781 ataccctgct ggagaaatac gcggattacg gcattggcga gttcgaccag ttgccgcgcg
      841 tgttgcaggt ttcgccgttt gaccagtatg ggacgacgca tgatatctac cgtttgtttg
      901 gcggggcgga gaagttgttg caagcggtgg atgagctgca gaagtttttg tatgaataaa
      961 atcgtctaaa ggtgtttgac atgaaaccaa acaatttaag tgaagaaaaa ataaaaagta
     1021 ctttacttaa aacaaaagtt acgcttgagg ctattgtttc ttttgtagaa gaatttgatc
     1081 ggggctcatt atcgctggtt ctccagcaaa atttttatga aaggttagca tcttttagag
     1141 aaaccctatt tcatttgcaa agaaatacaa gtgatatgat ttcatctcta gaaaacaaaa
     1201 atgaagaggt aacccaattg caaagcgaaa ttcaggaatt gcaaaggcga ctgcacgaat
     1261 tggaggcatt tgagaaacgc tctactcagg acggtggtgt gtttatcagt tattcgcatg
     1321 ctgacaagga ttttgtaaat gagattgtga aagaatttga aaccgataaa ataaattact
     1381 ggcttgatga taaggatttg ttcattgggc aggttattga taaggcaatt tcagatggaa
     1441 ttcaaaaaag ttggattttt atcactgtac tttcaccttc ttcagtaaac agccgatggg
     1501 ttgaacgtga attcgatgaa gcttcacatg aagaagtgga aggtcgaaaa attatcttgc
     1561 ctgtagtttg taataatttg ccagcagata aagttccagc cagaatacgc cgaaaattgt
     1621 atgttgatct ttccaaggat tttccgagtg gctatcaaaa gctcaagaaa tcaatactcc
     1681 actacttgga agaatataag aaaaatcaga aataacaatt acttattgga aacctcatgc
     1741 caagaacaaa gaagagcaca aacccagccc cctccgccgc taaggcggca cctcccccaa
     1801 aatccaaaaa acggatttta ggggaggaaa agcaacacaa caccaaagcc tccctctcat
     1861 ccctcatcaa atcggcgcgg gatattatgc ggaaggatgc ggggttgaat ggggatttgg
     1921 atagactgcc gcagttgtcg tggatacttt tcttgaagtg ttacgatgat ctggagatgc
     1981 ggcgagaaga agatgcgaag ttagagcgga aggcatataa gcctgttatt cctttgccgt
     2041 atcggtggcg ggattgggcg tcggatgagg ataacggggc gacgggcgag gatctggtca
     2101 agtttgtgaa tgacgggttg ttgcctgctc tgcgcgggct gtcggggtcg gatgaacgct
     2161 tgaaggtggc ggaggtgttc aaggagacgt ataaccgcat gttgagcggg tatctgctgc
     2221 gggatgtggt gaacctggtg gataacatcc actttcagtc gaaggatgag atctttacgc
     2281 tggcgcattt gtatgagtcg atgttgaagg agatgcgcga cgcagcaggc gattcgggcg
     2341 agttctatac gccccgcccc gtggtcaaac tgatcgtaga cagagtcaag ccgcggctgg
     2401 gagagagcgt gctcgatccc gcttgtggca cgggcggatt tttagtcgag tcgtatgagt
     2461 accttaagcc gcgcgtcaaa aattccaagg agcaggaggt gctgcaaacg cggacgttgt
     2521 ttggggtgga gaagaagccg ctgccgtatc tgttgagcac gatgaacctg ttgctgcatg
     2581 ggatcgagac gccgaacatc cgccgcgata acgcgttgaa gaatccgctg aacgagatcg
     2641 gggagaaaga ccgcttcgat gtgatcgtga cgaacccgcc cttcggcggc gaggaggagg
     2701 cgggcattca gttgaacttc cccgagaata cgcgcgccag cgagacggcg ttgttgttca
     2761 tgcagttcgt gatgcgttcg ctacggcacg gcggtaggtg cggcgtggtg gtgccgaatg
     2821 gatttctgtt cggcgacggg gtggcggcgc gggtgaaggc gcagatgctg agggacttca
     2881 acctgcatac gatcatccga ctgcccaacg gggtgttcgc gccttatacg tccatcccga
     2941 ccaatttgat tttcttcgat cggactcagc cgacgactga tatttggttc tatgagcatg
     3001 ttctccccga aggcgtgaag aactatacca agacgcgccc gatgcagttc gaggagtttc
     3061 agccgatttt ggattggtgg gatgaccgag ttgagaatga acgcgcatgg aaggtacgcg
     3121 ccgcagacgt gatccagact aacggcgggg gcgatgtggt gagcgtcaat cttgacatca
     3181 agaaccccag ccgcaaagag gacttggaac acctgccgcc tgccgagttg gcggagagta
     3241 ttctcgccaa ggagaagcag attgcggcga ttatggaaga aatcaagtct ttgctggcaa
     3301 aggcacaata atgagcaagt atcccaaagt gaaatttggt gattttgttg aacatattgt
     3361 tcgcgaagtt gaattggaag cggacaaaga atatccttct gttggtgtga agtggtatgg
     3421 ggaaggagtt cacattcatg aaatcaaaag tggtggaagt ttcaacgctt caagatttga
     3481 aataaaagcc aacgatatta tttacaatga tatgtgggca agaaatggtt cagttgcaat
     3541 tgttccttcg cacttagatg gcacagttgc ttcatcgcat tttccaacat tcttgccaga
     3601 cttgaccaga gttttaccca aatatttaga gttttatttc aagacgcgaa cattttggga
     3661 tgattgtgaa aatgcttcaa gaggcagtac agggcgaaac caaatcaaga agagcacttt
     3721 ttatgcaatt gaaattcccc tgccgctgct gacggagcaa caccgcattg tggcgcacat
     3781 cgagtcgctg gcggcgcggg tcaatgaggc acagcgt
//
LOCUS       DAOQNJ010000220         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_15829_length_3814_cov_9.575951, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000220 DAOQNJ010000000
VERSION     DAOQNJ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15829_length_3814_cov_9.575951"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLG52_07400"
     CDS             complement(<1..488)
                     /locus_tag="PLG52_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41309.1"
                     /translation="MENQTQEPNPILQIAWTRFAQMDAYSSRRTKNHLNFRRWLGILS
                     ILATLFAIIVSLFPSDNPSLIVVVFKFLLIMTPILSSGLAVYGSKFLSSGDWLVTRAG
                     AEEILKEIYTYRTVLKNSRTRDAWLENRLAEIQRSVFRGLNGELTIPPFEGYLPPHYN
                     QS"
     gene            complement(506..1948)
                     /locus_tag="PLG52_07405"
     CDS             complement(506..1948)
                     /locus_tag="PLG52_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNB41310.1"
                     /translation="MFVTKNLYQNDEAWKNVPLGFSSQTIGSWGCLLTSVTMMLNGIG
                     YNETPDTVNTKMKNAGGFQGPLFIPSVLPYVWGNCAYRDMQPCESVPAPLQQIDDAVA
                     SGKPVILQVDWNKQAGIQTHFVLVKEKKGDDYVLYDPYKYGGDGPDKEVLLTTRYKYN
                     GAKLSTEISAVLWFENYGINPPEPPAKVPVPAENFILYAAEDDLALRSQPATTGYLWK
                     RMVMNTELVCLEPKAQAAAKIGVQGQWIQVQDPKGDQGYVAGWFVSDKKAVPAPAAPV
                     TPASPSTSKPGTAPKPSSSASKPGSAPAPTPSKPAPVPPGALAFFPTEEVSFRSQPVV
                     ADNTLIRKIPVTEQLVSTEPADKVIPKVGVQNQWLKVRAADNKEGYVAAWYVKYAGGS
                     TAQANAAAAPAPAASTGGAIKVRTVAEAVSLRKQPVVSDATLIKRVPLGTEFTITEAG
                     GEAKIGKNDQWIKVKDASSEGYVAAWFVAR"
     gene            complement(2152..3477)
                     /locus_tag="PLG52_07410"
     CDS             complement(2152..3477)
                     /locus_tag="PLG52_07410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNB41311.1"
                     /translation="MKRVYQYLSVYVFLALACSSLTPSTSTGTGYRYDNHPDANTQSA
                     EYRALSKWGKTDITYYFINGTDKISGDREQELVAQAFALWAAQTSLTFTETNSRQSAD
                     IVIGWAEGEHGDGDPFDGPGDVLAHASYPNPYEERQVFLHFDDAERWVDSDTQDVDLL
                     TVAAHEIGHALGLGHSNDPNSLMYPSYSEPHRFLGGDDIAGIQSLYGLASEPPTAPEV
                     PKPNENPPTSPSQDSDGDGLADSDETLITGTDPNNKDSDSDGIGDGVEVVNRMNPLDA
                     DMDKDGVKDGAEIAAGTNPFLPDQANGVSPELSKEVGEFLTKAIKLQIEAYRQGDASV
                     ASVIMAGDVLQSLQGEISSLNQQGLVEIADIDYYKSYIDDIRIISNAQLEVDTCEVWS
                     LQYYRRSDGALVQSNEPTLLPQTITIQKIENGWFITAVQFFEAPAFCHS"
     gene            complement(3497..>3814)
                     /locus_tag="PLG52_07415"
     CDS             complement(3497..>3814)
                     /locus_tag="PLG52_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB41312.1"
                     /translation="FHGENAMELYYMSQAGISAMDCIVASTKNAARALGWDSKLGTLK
                     AGKLADLIVVKKNPLDDLRSLANKNNIEYVMQGGKFVARQFVEPTGIPEELMAGAWIC
                     CGF"
BASE COUNT          922 a          938 c          971 g          983 t
ORIGIN      
        1 tctgattggt tgtagtgagg cggtaaatag ccttcaaagg gcgggatggt caattcgccg
       61 ttcaaaccgc gaaagaccga acgttgaatt tcagccaagc gattttccaa ccaggcatcg
      121 cgagtgcgtg aattttttag aacggtgcga taggtataga tttctttgag aatttcttca
      181 gcaccagcac gtgtgaccaa ccaatctcca ctggagagga atttgcttcc atagaccgct
      241 aacccggaag aaaggatggg ggtcataatc aacaggaatt tgaagaccac aacaattagc
      301 gatgggttgt ctgaagggaa tagcgaaaca atgatcgcga acaaggtcgc cagaatactg
      361 agaatgccga gccaacggcg gaagtttaag tgattcttgg tgcgacgcga cgaataggca
      421 tccatctgcg caaaacgggt ccacgcaatc tggaggatgg gattgggttc ttgagtttgg
      481 ttttccatga gtatcctctt tatgcctaac gagctacgaa ccaggctgca acataacctt
      541 cactgctcgc atccttgacc ttgatccact gatcgttctt gccaattttg gcttcgccac
      601 cagcttcggt gatggtgaac tctgtgccga gcggaacacg cttgatgaga gtcgcatcgc
      661 tgacaacggg ctgtttgcgc aaagagacgg cttctgccac ggtgcggact ttaatcgcgc
      721 caccggttga agcggcaggg gcaggagcgg ctgcagcatt tgcctgagct gtggaaccac
      781 cggcatactt cacgtaccaa gctgctacat acccttcttt gttatcagcg gcacgcacct
      841 tgagccattg attttgcacg ccgactttcg gaatgacctt atcggcaggc tctgtgctga
      901 cgagttgttc ggtcacaggg atcttgcgga tcaaggtgtt atctgcaacg acaggctgag
      961 atcggaagga gacttcttcg gtggggaaga atgccaaggc gccaggagga acaggcgcag
     1021 gtttgcttgg ggttggggca ggagccgaac cgggtttcga ggcggaagaa gaaggcttgg
     1081 gagctgtacc cggcttcgac gtcgaaggag aggcgggcgt tacaggagca gccggagctg
     1141 gcacagcctt tttgtcagaa acgaaccagc cagccacata tccctgatcg cctttcgggt
     1201 cttgtacctg aatccattga ccttgcacgc cgatcttggc ggcggcttgg gctttgggtt
     1261 caaggcagac gagctcagta ttcatcacca tgcgcttcca caggtagcca gtggtggcgg
     1321 gttgagaacg cagagccaaa tcatcttcgg cggcatatag gatgaaattc tcagcaggaa
     1381 cgggaacctt ggcaggtggt tcaggcgggt tgatgccgta gttttcgaac cataatacag
     1441 cactaatctc ggtggagagt ttggcaccgt tgtatttgta gcgggtggtt agcaatactt
     1501 ctttatctgg accgtcgccg ccgtatttat acgggtcata aagcacataa tcatcgccct
     1561 tcttttcttt cacaaggacg aagtgagttt gaataccagc ttgtttgttc cagtccactt
     1621 gcaaaatgac gggtttgcca gaagcaaccg catcgtcgat ctgttgcagc ggagccggaa
     1681 cagattcaca aggctgcata tcgcggtatg cgcagtttcc ccaaacgtaa ggcaacacac
     1741 tgggaatgaa gagcggaccc tgaaaaccac ccgcgttctt catcttagtg ttgaccgtat
     1801 cgggcgtttc gttgtacccg atgccgttca acatcatagt cactgaagtg agcaaacaac
     1861 cccaagaacc gatggtctgc gatgaaaacc cgagcggaac gttcttccag gcttcatcat
     1921 tttggtacaa gttcttcgta acaaacataa aaaattgcct cctccatttg gagattggga
     1981 atttcaatct tgctttttaa ggataaattt atctaattct actcgatttg cctttgcaac
     2041 gattttgcaa tattttaagg tggatgccgc ccggctctct ctccgccgcc tcttggctca
     2101 ccccattggc agttttttcc ctcataaatc gacctgccaa tggggcgcat tttacgaatg
     2161 gcagaaagcc ggtgcctcaa aaaattgaac cgcagtgata aaccagccat tctctatctt
     2221 ttgaatagtg atcgtttgtg gcaatagcgt aggctcattg ctttgaacaa gggcaccatc
     2281 tgaacggcga taatactgca aagaccagac ttcacaagta tccacttcaa gttgagcatt
     2341 gctgatgatt cgaatatcgt cgatatagct tttgtaatag tcaatgtctg cgatctcgac
     2401 caacccctgt tggttgagcg aggaaatctc tccttgtaga gactgtagca cgtcgccagc
     2461 cataatcaca gacgcaacag aagcatcgcc ttggcgatag gcttcaattt gcagcttgat
     2521 cgccttcgtg agaaattcgc cgacttcctt cgaaagctcc ggcgagactc cattggcttg
     2581 gtctggcagg aaaggattgg ttcccgccgc aatttccgcg ccgtctttca ctccgtcttt
     2641 atccatatca gcatcaagcg gattcatgcg gttgaccact tctacgccat cgccaatgcc
     2701 atcactatct gaatctttat tattggggtc tgtacctgtg atgagagttt cgtctgagtc
     2761 tgccagccca tctccatcag agtcctggct tgggctggtg ggcgggtttt catttggctt
     2821 gggcacttct ggcgcggtag gtggttcact tgccaaccca taaagtgatt gaatgcccgc
     2881 aatatcatcc ccgcctagaa aacgatgcgg ctcactatag gatggataca tcagagagtt
     2941 cgggtcgttg ctgtgaccta gtcccaacgc atgaccgatc tcatgtgctg ctacggtaag
     3001 caggtctaca tcttgagtat cactatctac ccagcgttcg gcgtcgtcaa aatgtaaaaa
     3061 gacttggcgc tcctcatacg ggttggggta agaggcgtgt gctagtacat cgccaggtcc
     3121 gtcaaatggg tcgccatctc cgtgttcacc ttctgcccag ccaatgacaa tatccgcgct
     3181 ttgtcttgag ttggtctctg tgaaagtgag tgaagtttgc gctgcccata gtgcaaatgc
     3241 ctgagccacc aattcttgct cacggtctcc tgaaattttg tcggtgccgt tgatgaaata
     3301 ataggtgata tcggttttgc cccatttgga aagtgcgcga tattcagccg actgtgtatt
     3361 ggcatccgga tgattatcgt atcgataacc tgttccagtg gatgtactcg gtgtaaggct
     3421 ggaacaggca agtgcgagaa aaacatatac agacagatat tgataaacgc gtttcatttt
     3481 ttaacctcca ataaaactaa aatccgcagc agatccaagc gccagccatc aattcttcag
     3541 gaatgccggt cggttctacg aattggcgcg ccacaaattt accgccttgc atgacatatt
     3601 caatgttatt tttattggct aaagaacgca ggtcatctaa cggattcttt ttgaccacga
     3661 tcagatcggc taatttaccg gctttcagcg tgccaagctt tgagtcccag ccgagtgcgc
     3721 gagcggcatt cttcgtagaa gcaacaatac agtccattgc agagattccc gcctgcgaca
     3781 tgtaatatag ctccatcgca ttctcgccat gaaa
//
LOCUS       DAOQNJ010000221         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_16038_length_3784_cov_10.140520, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000221 DAOQNJ010000000
VERSION     DAOQNJ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16038_length_3784_cov_10.140520"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1482)
                     /gene="murF"
                     /locus_tag="PLG52_07420"
     CDS             complement(64..1482)
                     /gene="murF"
                     /locus_tag="PLG52_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNB41313.1"
                     /translation="MLTLADVLEALTTIRPANASTIITEGAIDSRQVIPGSLFVAIPG
                     EKTDGHDYLTDAFKRGASFALIQRDVDASFRTLDLRAGLSTDATLNFESPLCLRVDNT
                     ISALQQIARFWRRKLPNLRVVGITGSVGKSTTKETVAEVLSLRYRTLKSPGNLNNEIG
                     LPLTILRLGTGYDRAVLEMGFYVPGEIQFLCDIAQPQMGVVSNVGTVHAERAGSQEAI
                     FRGKSELPASLPADGVAILNFDDQWVRKMEEKTKARVFFYGLSPEAHLWADQIEGLGL
                     DGIRFRMHYGKEIIHMRVPMIGRHSIHTALRAAAVGLNDGISWQEILEGLSHGHTQLR
                     LVAARNETGALILDDTYNAAPESMLAALNLLDELDGRKIAVLGDMLELGPYERQGHEM
                     VGMRAAQVAKVLITLGTRGHMIAEAARRAGMKASAIMEYEDIAPIIDWLTKNLNSNDA
                     VLIKGSHGLRMDRIAAALEVRS"
     gene            complement(1484..3232)
                     /locus_tag="PLG52_07425"
     CDS             complement(1484..3232)
                     /locus_tag="PLG52_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNB41314.1"
                     /translation="MRQQYGRRSQVLAAALAFFAFAIVFQMTRIQNSPEADTFRQLSK
                     DYSYEWRTFYPNRGEIYDRNGRLLAGEKIVYEIGVDLNIMTDAHAIAFAVADALGMNY
                     DNLMSVIQNPPDGLSYIVLADYVESSQANYLQDLKKALNEQAPEGSMGGLTGLQFKSH
                     PQRSYPENSLASNILGFVNREGKGYFGVEEKYDSLLAGNPVTVLVPTDPNKAFEIPNA
                     PDGTTLVLTINRDLQSAAEEILDNALGTYGAQDGIIAIMDPQSGEMLAVATSRRMDLN
                     EFWNYGTIYNNATEFNPIISKTYEPGSVMKILTMAAALDSNTVTPETTYLDTGEILIG
                     GVTIQNWDRNPWGVQNMVGCLQHSLNICMVTISSQMGANTFYKYMDAFGIGHLTGVDL
                     AWEAPGRLKVPGDADWYPVDLGTNSFGQGVAVTPIQMMSAVSAVANNGRMVIPHVLYA
                     MVRDGRQYEVPDQFGPTPISEQTSRTLSEMLAVSLESESSAALIPGYRVAGKTGTAQI
                     PVDGFYDSTQTNTSFIGWGPVDDPKFMIYVWLERPTASPWGSETAAPVFAEMAQRTVV
                     LLDIPPDSIRLQVAQK"
     gene            complement(3229..3708)
                     /locus_tag="PLG52_07430"
     CDS             complement(3229..3708)
                     /locus_tag="PLG52_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41315.1"
                     /translation="MNIPNVNLPNVTHVIHAYRVAPWRVQRQWIGNALLVVVSLAMVA
                     ALYLNVTSRTAIAGREIQDLTDSIAASQQVSSDLQTQLANLTSAEVMQQRAKEMGFRP
                     VEPVELEYLVVPGYVAKDAVNLSSSAQLQLNVLAIPPEYNQSLLDWMDERLVARRYQ"
BASE COUNT         1028 a          931 c          895 g          930 t
ORIGIN      
        1 ttccccaaat ggcagtcatg ataaaagcca accccgccag gcttagggaa agagcaatgt
       61 ctctcacgag cgtacctcca atgcagcagc gatgcgatcc atgcgtaagc catgagaccc
      121 tttaattaac actgcgtcat ttgaattcaa attctttgtc aaccaatcta tgatcggggc
      181 aatgtcttcg tattccataa tagcagaagc cttcataccg gctcgacgag ccgcttccgc
      241 aatcatgtgt ccacgggtgc ccaaggtaat gaggactttc gccacttgag ccgcacgcat
      301 tccgaccatt tcatgtccct ggcgttcgta gggaccaagt tcaagcatat cacctaacac
      361 ggcaatcttg cgaccgtcta attcgtctag cagatttaac gctgccaaca tagactcagg
      421 cgcagcattg tatgtatcat ccaaaatcaa agcgccggtc tcattacgag cagctaccaa
      481 gcgcagttga gtgtgcccat gggagagacc ttctaaaatt tcttgccaag aaatgccatc
      541 attcagccca acagcagcag cgcgtaatgc cgtatgaata gaatgacgcc caatcattgg
      601 cacgcgcatg tggataatct ctttaccgta atgcatacgg aagcggatgc catctaagcc
      661 cagcccttcg atctggtctg cccacaaatg tgcttctgga gaaagcccat agaagaaaac
      721 gcgagccttg gtcttctctt ccattttgcg gacccactgg tcatcaaaat ttagaatcgc
      781 aaccccatca gctggaagag aggctggtaa ttcgctcttg ccgcggaaaa tagcttcctg
      841 tgacccggcg cgttccgcat ggacagtacc tacatttgaa acaacaccca tttgcggctg
      901 agcaatatca caaagaaatt gaatctcacc aggcacgtaa aatcccattt caagcacagc
      961 acggtcataa cctgtaccga gtcttaatat ggtgagcgga agtccaatct cgttatttaa
     1021 attgccaggg cttttgagtg tgcggtaacg aaggcttaac acctctgcta cagtttcttt
     1081 ggttgtagat ttacccacac ttcctgtgat tcctaccacg cgcaggttcg gcaatttacg
     1141 gcgccagaaa cgcgcgattt gttgtaaggc tgaaattgta ttgtccacgc gcaggcagag
     1201 aggagactca aagttcagtg tggcatcagt tgatagacca gcgcgcaggt cgagggtccg
     1261 gaaagaagcg tctacgtctc gttggattaa agcaaaagaa gcccccctct tgaaggcatc
     1321 tgtcagatag tcatgtccat ctgttttttc gccgggaatg gcaacaaaaa gagaacccgg
     1381 aatcacttgg cgcgaatcga tggcaccttc tgtaatgatt gtagaggcat ttgcagggcg
     1441 aatagtggta agagcttcaa gaacgtctgc tagagtaagc atattatttt tgagcaactt
     1501 gtaggcgaat tgaatcagga gggatatcta acaagaccac ggtccgctgc gccatttcag
     1561 caaaaacagg ggcagcagtt tcagaacccc aaggtgaagc tgtagggcgt tcgagccaaa
     1621 catagatcat gaatttcggg tcatctactg gcccccaacc gatgaaagag gtatttgttt
     1681 gcgtgctgtc gtaaaaacca tcgactggaa tttgcgctgt accggtctta ccagccacgc
     1741 gatacccagg gatcaatgca gcagatgatt ctgattcgag agaaaccgct agcatttcag
     1801 ataatgtacg agaggtctgt tctgagatgg gagtaggacc gaactgatct ggcacttcgt
     1861 attggcgacc atcgcgcacc atggcataca aaacatgtgg gatgaccatg cgtccgttat
     1921 tggcaactgc agaaacggca gacatcattt ggataggagt gactgctaca ccttgaccaa
     1981 atgagtttgt accaagatcc acgggatacc aatccgcatc tcccgggacc ttgagcctgc
     2041 ctggtgcttc ccaagccaga tccacgccgg tgaggtgacc aattccgaaa gcgtccatgt
     2101 atttatagaa agtattcgca cccatttgtg aagaaatggt gaccatacaa atattgagtg
     2161 aatgctgcaa acaccccacc atgttctgca caccccacgg attgcgatcc cagttttgaa
     2221 tggtcacacc gccgatcaga atctcaccag tgtcgagata agtcgtttca ggggttacag
     2281 tattgctgtc taaagcggca gccatcgtaa ggatcttcat cacggaaccg ggttcatagg
     2341 ttttggaaat gatcgggtta aactcggttg cattgttata aatagtgccg taattccaaa
     2401 attcattcaa atccatacgg cgcgatgtag caacggcaag catctcgccg ctttgtggat
     2461 ccataatggc gataatgcca tcttgtgcgc cgtaagttcc taaagcatta tcaagaattt
     2521 cttctgctgc agactgcaag tcgcggttga tggtaagaac caatgtagtg ccatcaggcg
     2581 cattgggaat ttcaaaggct ttgtttgggt ccgtcggcac caatacagtg acaggattgc
     2641 ccgctaataa actatcgtat ttctcttcta caccgaagta tcccttgcct tcacgattaa
     2701 caaacccaag gatatttgaa gccagtgagt tttcgggata actacgttga gggtgagatt
     2761 taaactgcaa gcccgtcaac ccgcccattg aaccttcagg ggcttgttcg ttcaatgctt
     2821 ttttgaggtc ttgcaaataa ttcgcttgtg aagactcaac ataatctgca aggacaatgt
     2881 atgagagccc atctggtggg ttttgaatga ctgacatcaa attgtcgtaa ttcataccaa
     2941 gagcatctgc tactgcaaat gcaatggcgt gagcatccgt cataatgttt aagtctacgc
     3001 caatttcata aacgatcttc tccccagcca acaagcgacc gttacggtca tagatctcgc
     3061 cgcggttggg gtaaaacgtg cgccattcgt atgaataatc tttagaaagc tggcgaaagg
     3121 tatcggcttc ggggctgttt tgaatgcgcg tcatctgaaa cacgatggca aacgcaaaga
     3181 aagccaatgc ggcagctaag acttgactgc ggcgcccgta ttgctgtctc attgatatct
     3241 ccgtgccacc aagcgttcat ccatccaatc caaaagtgac tgattgtatt caggcggaat
     3301 ggcaagcaca ttcaattgaa gttgagcaga ggaggaaaga ttgactgcgt ctttagcaac
     3361 atagcccggc accacaagat attccagttc aacaggttcc acaggtcgga aacccatttc
     3421 ttttgcacgt tgttgcatga cttcagcaga agtcaggttt gccagttggg tctgaagatc
     3481 gctactcacc tgttggctgg cagcaataga atcggtcagg tcttgaattt cacgaccagc
     3541 aatagcagta cgagacgtta catttaaata aagagcggca accatggcaa ggctcaccac
     3601 aaccaataac gcattgccga tccattggcg ttgcacgcgc caaggagcga cccggtaggc
     3661 atggatcacg tgagtaacat tggggaggtt gacgttcggt atgttcactt taattctctc
     3721 tcaattgcaa gcgaggaaga cctcgcccct acaacttctc aacaactcta agtttggcac
     3781 tgcg
//
LOCUS       DAOQNJ010000222         3774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_16101_length_3774_cov_10.511966, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000222 DAOQNJ010000000
VERSION     DAOQNJ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16101_length_3774_cov_10.511966"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PLG52_07435"
     CDS             <1..600
                     /locus_tag="PLG52_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNB41316.1"
                     /translation="VHLATPHGVVLQQPAFSKYYLHLGEISSYPPGYKENAGIFCHTN
                     PWIMIAEAKVGRGDKALDYYLRINPSAREGIGDLHRCEPYVYAQMIAGKDAATHGEAK
                     NSWLTGTAAWNYVAITQWILGIRPTYEGLQVSPVVPVGWKSFEAVREFRSVRYNIKVE
                     RSGAGNQVKLEVDGKAIAGNIIPLAANGTKEVRVRVILS"
     gene            complement(648..1349)
                     /locus_tag="PLG52_07440"
     CDS             complement(648..1349)
                     /locus_tag="PLG52_07440"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNB41317.1"
                     /translation="MSVSLAMQRYAAEIYRLQQDHEQVPLSLLSSHVDASAQAISNMV
                     KRLQKLGYLVHEPYRGVRLTETGEKIAMPSLRRHRLTEVFLVKVMKYDWATAHELSDT
                     FEKGVNDEIENRMDELAGRPTRCPHGEPIPSKDGFMPVVKDMPLIDVPSGSDCVISRV
                     RTHDLEKLNYIGELGLTPGKQFHLFSCAPFKGPLRLQMKPHDHLIGYELAASLWVEVT
                     MKGEGNKVPTSLASK"
     gene            1513..2052
                     /locus_tag="PLG52_07445"
     CDS             1513..2052
                     /locus_tag="PLG52_07445"
                     /inference="COORDINATES: protein motif:HMM:NF017987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HNB41318.1"
                     /translation="MFQTNISLSPEMTANERNQAIREAYNQDRSQMTLMLAHQLRIPE
                     VDILRALEGDTARELDFARWEEIIRAFEALGEVTVIVSNGSTTIESFGQFGGFSNAQG
                     FLNVRSKSLDMHIRGWELASVFAFRKPSHLDKHESLSFQFFDKRGNSAFKVFLNFGGK
                     DPAPELVEAYNELIGRFSK"
     gene            2192..3496
                     /locus_tag="PLG52_07450"
     CDS             2192..3496
                     /locus_tag="PLG52_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HNB41319.1"
                     /translation="MFIKRLQLALIHTAVAMTLVPINSTLNRVMIFDLGVSKTLFTLL
                     AIFPYLLSPIQVAIGSFSDRHPILGYRRAPYIFAGLLLCVIGVAVSPQIAILISNNFT
                     VGVAAGILAFGLWGMGYNLSAVSYLSLASELSGEKERGKTVATMFTLMVIGLIATGIS
                     LSQMVPTFEPATLQRAFLIVAASALTMGLIGLIKLEPRFDHSAQAPKADTYTVKQMMA
                     AITENPVAKIFFVYLLLLLAAILSQDVLLEPFGAQAFGMTLEQTSRIVSITNSFTLIA
                     FIIAGFLDGRVKKKYVAQTGNLAALFGFIVIVISGLIANKNAFYVGITLLGFGTGIST
                     IANLSLMFDLTVPEKVGLYIGAWGFSNGLSRLVGLLMAGVIADVATKITGDALHGYLV
                     VFGLEALMLFIAAIMLYRIDVGVFQKKAHEPGFAEKIALASE"
     gene            3524..>3774
                     /locus_tag="PLG52_07455"
     CDS             3524..>3774
                     /locus_tag="PLG52_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB41320.1"
                     /translation="MTNDEWLSLAGAADLLGVHPSTVRLWSDKGLLPVHKTQGGHRRY
                     KRGEILLWAESNEKSKEEALEPEGMMQEVIKNVRMQISEG"
BASE COUNT          921 a          973 c          909 g          971 t
ORIGIN      
        1 gtccatttgg cgacaccgca cggagtggta ttacaacagc cagccttcag caaatattat
       61 ttgcacttgg gtgagatctc ttcctacccg ccgggctaca aggagaacgc gggtattttc
      121 tgccatacca atccatggat catgatcgca gaggcaaaag ttgggcgcgg agataaagcg
      181 cttgactatt acctacgcat caacccatcg gcgcgggaag gcatagggga tttgcatcgc
      241 tgtgagccgt atgtctatgc tcaaatgatc gcgggcaaag atgctgcgac tcacggcgag
      301 gcaaagaatt cctggctcac tggcactgcc gcttggaatt atgtcgccat cacgcaatgg
      361 attcttggaa tccgcccgac gtatgaaggg ttgcaagtct cgccggttgt tccggttggg
      421 tggaaatcgt tcgaagcggt gagagaattt agaagcgtac ggtacaacat caaggtcgaa
      481 agaagcggtg ctgggaatca ggtaaagctg gaagtggatg gaaaagccat cgcaggaaat
      541 atcatcccct tggctgcgaa tggaaccaaa gaagttcgtg tgcgtgtgat attgagctag
      601 tgattttctt gataaaaaaa cacatcccga aatcgggatg tgttttttta tttagacgcc
      661 aggcttgttg gcactttatt tccttcgccc ttcatggtca cttccaccca aagagaagcg
      721 gcgagttcgt agcctatgag atggtcatgc ggtttcattt gtaatcgtaa cggacctttg
      781 aacggggcgc aactaaacaa gtgaaattgc ttgccggggg taagacctaa ttcgccaatg
      841 taatttaatt tttcaaggtc atgggtacgg acgcggctaa tgacacaatc agaaccagag
      901 gggacgtcga tcaatggcat atctttgaca acaggcatga agccatcttt ggaaggaatc
      961 ggctcgccat gcgggcaacg tgtggggcgc ccggcaagtt catccatgcg gttttcgatc
     1021 tcgtcgttga cgcctttttc gaaggtgtca gaaagttcgt gggcagttgc ccagtcgtac
     1081 ttcatcactt tgacgaggaa aacctcagtg aggcgatgcc tacgcaggga aggcatggcg
     1141 atcttttcgc cagtttcagt aaggcggact ccgcgatagg gttcatgtac aagataaccc
     1201 agcttttgca aacgcttgac catgttggaa atggcttgtg cagaagcatc cacatgcgaa
     1261 cttaaaagcg aaagcggcac ctgctcatgg tcttgttgca ggcggtagat ctccgcggca
     1321 taacgctgca tagccaaact tacagacatt ctttaaaaca ctccaaaatc ttcaattggg
     1381 ttgaaaactg gcttcaactg gatatagata aattttataa caattatcta gattattcgt
     1441 accgccgtga ttatactacg cgccacttat caaacaaagt atggtcaaca ctacatttta
     1501 ggagaatatt tcatgttcca aaccaatatc agcctcagcc ccgaaatgac cgccaacgag
     1561 cgaaaccaag cgattcgtga agcgtataac caagaccgct ctcaaatgac actcatgctt
     1621 gcccaccagt tacgcatccc cgaagtggac attctgcgag cgctcgaagg cgacaccgcc
     1681 cgtgagcttg attttgcgcg ctgggaggag atcatccgcg catttgaggc gttgggcgag
     1741 gtgaccgtca tcgtctcgaa cggctcgacc accatcgaat cgttcggtca gttcggcggt
     1801 ttttccaatg ctcaaggctt tctcaacgtc cgcagcaaat cactggatat gcacattcgc
     1861 ggttgggagc tggcatcggt cttcgctttc cgcaaaccga gccacctcga caagcatgaa
     1921 tcgctttcct tccaattctt cgataaacgc ggcaactctg ccttcaaagt ctttctcaac
     1981 tttggtggca aagacccagc tcctgagttg gtcgaagctt ataacgaatt gatagggcgt
     2041 ttttcaaagt aaatcttcct cagaaaactc tagtgaaatt ctatataaat ctatatactc
     2101 cttagtaaac ttgagcctac aatatcaacc aagaggtgag ggcgcccaaa ccgcccttcc
     2161 ttttgtttaa tcctcaacct caggaaatgc tatgttcatc aaaagacttc aacttgccct
     2221 catccatacc gctgttgcca tgacgcttgt gcccatcaac agcaccctca accgggtcat
     2281 gatcttcgat ctaggcgtct caaaaaccct ttttacactg cttgccatct ttccctattt
     2341 gctttccccc attcaagttg ccattggctc cttctcagac cgtcacccca ttctagggta
     2401 tcgccgcgcg ccatacattt tcgctggtct gcttttgtgt gtgattgggg ttgccgtctc
     2461 accgcagatc gccatcctca ttagtaacaa ctttaccgtt ggggtggctg ctggcattct
     2521 cgcctttggt ttatggggca tgggctacaa cctctctgct gtttcatatc tttcgctggc
     2581 atctgaactt tccggcgaaa aagaacgcgg taagaccgta gccaccatgt tcacactcat
     2641 ggtcatcggg ttgatcgcga cgggcatcag cctgagtcag atggtgccca cctttgaacc
     2701 tgccaccctt cagcgcgcct tcctgatcgt cgccgcctca gccctgacca tgggactgat
     2761 cggtctcatc aaactggaac cacgcttcga ccactctgcc caagccccca aagcggatac
     2821 gtacaccgtc aagcaaatga tggctgccat tactgaaaac cccgtagcga aaatattttt
     2881 cgtatacctg ctcttgctcc tcgccgccat cttgagtcaa gatgttctgc tcgaaccctt
     2941 cggcgcgcaa gccttcggca tgactctcga acagacctcg cgcatcgtct ccattactaa
     3001 ttcgttcaca ttgatcgcct tcatcattgc aggttttctc gatggacgtg taaaaaagaa
     3061 gtacgttgca caaacaggca acctcgccgc attgttcggc tttattgtca ttgtcatcag
     3121 cggactgatc gccaacaaaa atgcctttta tgttggcatt accctgctcg ggtttggcac
     3181 cggaatttcc accatcgcca acctctccct catgttcgac ctgaccgtcc ccgaaaaggt
     3241 tggtttgtat attggtgctt ggggcttctc gaacggactc tcgcgtttag ttggtctact
     3301 catggcaggc gtcatcgcag atgtagcgac caaaatcact ggtgatgccc ttcatggcta
     3361 tctggtggtc tttgggttag aggcattgat gttgttcatc gctgcgatca tgttgtatcg
     3421 cattgatgtg ggcgttttcc aaaagaaggc gcatgaacct gggtttgctg aaaagatcgc
     3481 gcttgcctca gaatagtttg aaaaagcagg atatcggctg aatatgacaa acgatgaatg
     3541 gctttctctc gctggtgccg ccgatctgct gggggttcac ccgagcacgg tgcgattatg
     3601 gtctgataaa ggcttactcc ccgttcataa gactcagggt gggcatcgcc gctataagcg
     3661 tggagaaatt ctcctttggg cagaatcaaa tgaaaagtcc aaagaagagg cgcttgaacc
     3721 cgaaggcatg atgcaagaag tcatcaagaa cgtccgcatg caaatctctg aagg
//
LOCUS       DAOQNJ010000223         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_16107_length_3773_cov_7.351802, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000223 DAOQNJ010000000
VERSION     DAOQNJ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16107_length_3773_cov_7.351802"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1291)
                     /locus_tag="PLG52_07460"
     CDS             complement(191..1291)
                     /locus_tag="PLG52_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNB41321.1"
                     /translation="MSDLSHAYAVIMAGGGGTRLWPLSRKDKPKQLLPLIGQETLFQS
                     TVLRLKELFPPERILVVTIEEQAQEMKQQVPELPEENYLIEPAPRGTASVVGLAAMAL
                     QKRDPEASMAILPSDHFIRNVDLFHYLLKAAFDVAADNYLVTLGITPTMPSTGYGYIQ
                     QGTPIGGGYKYPVYTVQSFKEKPDEATAQQLLRMGGHSWNSGMFIWRADAILNEIDKQ
                     MPNLGKALREIGDAWNSSHKIETLKKIWPDLKNETVDYGIMEKAEKVAVLPAGGLGWS
                     DVGSWSSLFEVLLPDMSGNIATNDALHLALETNNTMVYGRGSDRLVVTIGVDDTVVVD
                     TGDVLLICKTDQSQKVKDVVEHLKKHRQEKYL"
     gene            complement(1302..2408)
                     /gene="dprA"
                     /locus_tag="PLG52_07465"
     CDS             complement(1302..2408)
                     /gene="dprA"
                     /locus_tag="PLG52_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNB41322.1"
                     /translation="MDDKKYWIGFNLIKGIGAVRMQGLVAYFGDLESAWNADAPELME
                     AGLGAKLAEKVIGARKQIDLNQVWSKIEAQGIKILTWADEEYPNRLKEIDQPPPVLYI
                     RGEYLMDDLFAVAIVGTRKVTAYGRQVTEEIASFLAANGITVVSGLARGVDAIAHQTA
                     LKAGGRTIGILGSGVDRIYPPEHRGLAEQMMQRGAIISDYAVGTPPDASNFPPRNRII
                     SGLSLAVVVIEAAETSGALITAEFAAEQGREIFAVPGSILAPQSKGTNRLIQKGAQPL
                     LTPADLMQALDLTRMDKQKSARKILPSDETEARVLNTLSSEPLHVDEIRNLANLPIEK
                     ISAALAMMELKGMVRQVGGMNYVAVRDGQSDYMA"
     gene            complement(2446..2988)
                     /gene="rimM"
                     /locus_tag="PLG52_07470"
     CDS             complement(2446..2988)
                     /gene="rimM"
                     /locus_tag="PLG52_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNB41323.1"
                     /translation="MTTEQNSGSPKGESIYLAIGYLRRPHGVQGEVIMDLHTDFPDRI
                     KSGRQVYIGEKYEAFTIGSARVHGNGLLVKLRGFDSPETAGRFRNQWVYVKSTEVPAL
                     PEGQHYKHELIGLTVMTDDDTKLGLLNEVLETGANDVYVIVKEDGKEILLPAIPDVVL
                     NINMADKVMRVHLIDGLLDT"
     gene            complement(2988..3221)
                     /locus_tag="PLG52_07475"
     CDS             complement(2988..3221)
                     /locus_tag="PLG52_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNB41324.1"
                     /translation="MKELIEYIAKSLVDHPEEVIVTETKGNRPRLGLTVAKEDMGRVI
                     GKGGKVANAIRTLLRVAAERQGKQATLDVTEPS"
     gene            complement(3296..3637)
                     /gene="rpsP"
                     /locus_tag="PLG52_07480"
     CDS             complement(3296..3637)
                     /gene="rpsP"
                     /locus_tag="PLG52_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNB41325.1"
                     /translation="MVRIRLRRIGLKGQPTYRIVAADKEAPRDGRFLEILGVYNPRTN
                     PATIKLNEDRIYHWMKNGAQPTESAAQVLKTAGTLERFERFKKGEAVETLVAEAAAAE
                     AKRAAPVKTRK"
BASE COUNT          939 a          973 c          840 g         1021 t
ORIGIN      
        1 tcccgtttgg tcttgcactt catacaataa gcttccattt gtattcctcc aacatatctc
       61 tcacaaagca gaatttgcct cgagagttgt tcggttcgta taaaaaacat cccgcaaggt
      121 taaaccttac gggatggaca ttctacaaca agtttccgtt tttttgcaaa cgccaatttt
      181 tcgggcgatt ttaaaggtac ttttcttggc gatgtttttt gagatgttcc actacatctt
      241 taaccttttg tgactggtct gtcttgcata tcaacagcac atcgccagta tctacaacca
      301 ctgtatcgtc cactccaatg gtcacaacga ggcgatcaga tccacgtccg tagaccattg
      361 tgttgttggt ttcaagcgct aaatgcaaag catcattggt ggcaatattg ccactcatat
      421 caggcagaag gacttcgaac aacgaagacc atgaaccaac atcgctccac cccaaaccgc
      481 cagcaggcaa gacggcaact ttttcggctt tttccataat gccatagtcc acagtttcat
      541 tcttcaggtc gggccagatt tttttcaagg tctcaatctt atgagaagaa ttccacgcat
      601 caccaatttc acgcagggct ttacctaaat taggcatttg tttgtcaatc tcattcaaga
      661 tcgcatcagc acgccaaata aacataccgc tattccaaga atgcccaccc atacgaagca
      721 attgttgcgc agtggcttca tctggttttt ccttgaaact ctgcactgta taaacagggt
      781 atttgtaacc accgccaata ggcgtccctt gctgaatgta gccatacccg gtagaaggca
      841 tggtcggcgt aatgccaagt gtgaccaggt aattatccgc tgcaacatca aaagcagctt
      901 taagtagata gtggaacaaa tctacattgc gaatgaaatg gtcagacggc aaaattgcca
      961 tggaggcttc ggggtctcgt ttttgtaatg ccatggctgc taaccctaca actgaagccg
     1021 ttccacgcgg agcaggttca atcaaataat tttcttctgg caattcagga acttgctgtt
     1081 tcatttcctg tgcctgttcc tcaattgtca caaccaaaat gcgttcgggt gggaacaatt
     1141 ctttcaaacg caaaaccgta ctttgaaaaa gagtttcctg cccaatcaaa ggtaaaagct
     1201 gcttgggttt atctttgcgg gaaagaggcc acaaacgcgt cccaccaccg cctgccataa
     1261 tcaccgcata ggcatgtgat aaatctgaca tagtttttct cctaagccat gtaatcagac
     1321 tgcccatcgc ggacagctac ataattcata ccaccgactt gccgaaccat acctttcaat
     1381 tccatcatag ctaatgcggc tgaaattttc tcaattggca ggtttgccaa atttcgaatc
     1441 tcatccacat gtagaggttc actgcttaaa gtattcaaga ctcgtgcttc agtctcatca
     1501 gaaggcaaaa tcttgcgagc cgatttttgt ttgtccatgc gggttaagtc taaggcttgc
     1561 atcaagtctg ctggggtgag caaaggctgt gctccttttt ggatcaggcg attcgtgcct
     1621 ttgctttgcg gcgccaaaat gctacccggc acggcaaaga tctcacgccc ttgttctgca
     1681 gcaaattcag cagtgattag agcgccactg gtttcagcag cttcgatcac caccacagcc
     1741 aatgataaac ctgaaatgat tctatttctt ggcggaaagt ttgaagcgtc tggcggggtg
     1801 ccaacagcat agtcgctgat gatcgctccg cgctgcatca tctgttcggc aagcccacga
     1861 tgttctggcg gataaattcg atctactcca gaaccaagaa taccaatcgt tcgcccgccg
     1921 gctttgagcg cggtctgatg cgcgatggca tccactccac gggcgagtcc gctcacgacg
     1981 gtgatgccat ttgcggcgag gaaagaggcg atctcttccg tcacctgcct gccatatgct
     2041 gttaccttgc gcgtaccaac aatggcaact gcaaacaagt catccatcaa atattcacca
     2101 cggatgtaaa ggactggcgg aggttgatcg atttccttga ggcgattcgg atattcttca
     2161 tctgcccacg tgaggatttt gatgccttgc gcttcgatct ttgaccaaac ttgattcagg
     2221 tcaatttgct ttctcgcccc gatcactttc tctgccaatt ttgcgcccag accagcttcc
     2281 atcaattcag gggcatctgc attccaagca gactcaaggt cgccaaaata ggcgaccagc
     2341 ccctgcatgc ggacagcgcc tatgcctttg atgagattga agccaatcca atacttttta
     2401 tcgtccattg aaacgcgatt ctaacactcc ccgcttggtt ttgttttagg tgtcgagcag
     2461 accatctata aggtgaactc gcataacctt atcagccatg ttgatattga gaactacatc
     2521 tggaattgca ggcaaaagaa tctctttgcc atcttctttg acaatgacat acacatcatt
     2581 tgcgccagtt tcaaggactt cattcaaaag accaagtttt gtatcgtcat ccgtcatcac
     2641 ggtgagaccg atcaactcgt gcttgtaatg ctgaccttca ggcagtgcag ggacttccgt
     2701 cgatttaaca tacacccact gattgcggaa acgcccagcc gtttcaggag agtcaaaccc
     2761 gcgcagtttt accagtagcc catttccatg cacacgggca gagccaatag taaaggcttc
     2821 gtatttctcg ccaatgtaaa cttggcgtcc tgatttgatg cggtctggga agtctgtatg
     2881 caaatccata ataacttcgc cttgcacccc atgcggtcgg cgcagatacc caattgctaa
     2941 ataaatagac tcgccttttg gcgagcctga attctgttcg gtcgtcatta ggagggttcc
     3001 gtcacatcca aggtggcttg ttttccctgg cgttcagccg ccacacgcag gagggtgcga
     3061 atggcatttg ccactttgcc acccttgccg atcacgcgcc ccatatcttc tttggctacg
     3121 gtcagcccaa ggcgcggacg gttgcccttg gtttcggtca caatgacctc ttcggggtgg
     3181 tccactaatg atttcgcaat atattcgatc agttctttca ttttaaattt cctcatttac
     3241 acattacgga ttacgcagtc gaaattgcgt aatccgtaat gatgagtcct gcgatttact
     3301 tgcgggtctt gacgggtgca gcgcgcttgg cttcagcagc cgcggcttct gcaacgaggg
     3361 tttcaacagc ttcgcccttc ttgaagcgtt cgaaacgctc gagggtgcca gcagtcttga
     3421 gcacctgggc agcggattcg gtgggctgag cgccgttctt catccagtga tagatgcgat
     3481 cttcattcaa cttgatcgtg gcagggttgg tacggggatt gtacacaccc aaaatttcca
     3541 aaaagcgacc atcgcggggg gcttctttat cagcagcgac gatgcggtac gtgggctggc
     3601 ccttgagacc aatacgacgt aaacgaatac gaaccatctt aaacttgctc ctttagattt
     3661 tgattacgtt tcagacaggc actagagacg agagggatgt gcgggctgaa atcgcgggct
     3721 gtattttacc agagaaaaca ataaaaagac cccaatatcc ttggggtctg aga
//
LOCUS       DAOQNJ010000224         3754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_16236_length_3754_cov_7.947283, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000224 DAOQNJ010000000
VERSION     DAOQNJ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16236_length_3754_cov_7.947283"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..733)
                     /locus_tag="PLG52_07485"
     CDS             complement(368..733)
                     /locus_tag="PLG52_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41326.1"
                     /translation="MKFSFPVKIYLLALVLRLIPVFLTMNMGIGLDDMFQYDMLARSL
                     ASGHGFRWYALEDLERLEPYVSLDLSTASEYDPRYGVVFAGILIVLLALNWGFELHRD
                     ADKIAAILQPNGNQLYLPY"
     gene            complement(730..1248)
                     /locus_tag="PLG52_07490"
     CDS             complement(730..1248)
                     /locus_tag="PLG52_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNB41327.1"
                     /translation="MFTQPKSTASYLNPKCEARPKRVTGCAVFAIAPISKGELISLWG
                     GNIIHKSQLDQTMPRFTERVIQIDEDLYLLTSAEKEPNDCFNHSCEPNLGFTGQIGLI
                     AMRDIQTGEELTFDYAMSDGGPYDEFECDCGNPTCRHKVTGNDWALPELWQKYEGYFS
                     SYLARRIENLRK"
     gene            complement(1327..2142)
                     /locus_tag="PLG52_07495"
     CDS             complement(1327..2142)
                     /locus_tag="PLG52_07495"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41328.1"
                     /translation="MRSKSSDSASPALVLAAIGGLLFLHAPIWVIFLYSFTTDEATYT
                     FPLPGLTTKWFGAALQRVDLWNALTLSLQVAVVATLVALVLGTLAAAAVYRSDFFGKE
                     TISLLLILPIALPGIVTGIALRSAIGTLNIPFSYWTIVIGHATFCVVTVYNNVLARFR
                     RMQGSQVEASMDLGANSFQTFRYVVLPNIATALVAGGMLAFALSFDEVIVTTFTAGQQ
                     ATLPIWIFSQLVRPRDRPVTNVVATIVVLITFLPIFFAQRISASSADTEGDSK"
     gene            complement(2132..3037)
                     /locus_tag="PLG52_07500"
     CDS             complement(2132..3037)
                     /locus_tag="PLG52_07500"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41329.1"
                     /translation="MLNRISTYLYRHPRLVLGLLLGPPMLYMGVVYLGSLFSLLINSF
                     FYLEEFTGLVVREFTLQTYAKLLDPTNLGIFFRTATMALLVTIADAIIAFPLAYYIAR
                     FAPKKLKTYLYIAVTIPLWSSYLVRVYAWKLILAKEGIVSWFFNLFGLQFILDGILSI
                     PGIGGSSLSLSPIGMFIAFCYIWLPYMIVPIQTALERVPKSLLEASSDLGANPSQTFR
                     QVLLPLSFPGVVAGSIFTFSLTLGDFIIPQVLGNSRFFIGQAVYSYQGTAGNIPLAAA
                     FTMGPIVIMVVYLLIARKLGAFDAL"
     gene            complement(3030..>3754)
                     /locus_tag="PLG52_07505"
     CDS             complement(3030..>3754)
                     /locus_tag="PLG52_07505"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41330.1"
                     /translation="KVEKAERMKRVEEMLDLVQLSGFAGRKPSQLSGGQRQRVALARA
                     LINHPKVLLLDEPLGALDLKLRQQMQVELKSIQKRVGITFIFVTHDQEEALTMSDRIA
                     VFSQGKIEQIGTPSEVYEHPASQFVAGFVGTSNLISGDVAERITGHAQTFSIRPEKIH
                     IGSQQEYISSDMYAVDGTIRDVIYLGLYTRYLVEITQGIDLVVISQNLKTTSADVFSS
                     KGSAVRLSWQQDHISILGGKGA"
BASE COUNT         1139 a          825 c          910 g          880 t
ORIGIN      
        1 actgtatatg ttagtccgag ttttcattcg gcgattggaa actcggacta acatatacag
       61 taattggcgt cataactaag acgccaatta ctgtatatgt tagtccgagt ttccaatcgc
      121 cgaatgaaaa cccatattct tttggattct ctcgaaagat aagtaaggtt attagaagcg
      181 ggataaaaaa atacaaaacg atacggtcta tatatttata tgcaaatatc ttatggtaat
      241 agtcaaccat caacaacaag gtgctgataa tggtcactgt aacaaccttc caatcaaagc
      301 ttattttttc gcctgttaaa tatttcatgc ttacctctga gtataaagtc acccctgcct
      361 ctgggaatta atatggcaaa tacaattgat tcccatttgg ctgaagaatg gcggcaattt
      421 tatccgcatc acggtgtaat tcaaatcccc agtttaacgc caagagaact atcaatattc
      481 ctgcaaatac taccccatat cgagggtcat actcagatgc agtagacagg tctaaactca
      541 cataaggttc gaggcgttcg aggtcttcta aggcatacca gcggaatcca tgcccagagg
      601 cgaggctacg agccagcata tcgtactgga acatatcatc cagcccgata cccatgttca
      661 tggtcaggaa gaccggtata agtcgcaaaa cgagcgcgag cagataaatt tttacaggga
      721 aagaaaactt catttcctca aattttcgat ccggcgggca agataggacg agaaatagcc
      781 ttcatatttt tgccaaagtt ctggcaatgc ccaatcgttc ccggtcactt tgtgacggca
      841 agttgggtta ccgcaatcgc attcaaattc atcgtatggt ccaccgtcag acatggcgta
      901 atcgaaggtc aattcttcgc cggtttgaat atcacgcatg gcaatcaaac cgatctgccc
      961 cgtgaagcca aggtttggct cacatgaatg attgaaacaa tcgttcggtt ctttttctgc
     1021 ggaagtaagc agataaaggt cttcgtcaat ctgaatgact cgttctgtaa aacgcggcat
     1081 ggtttgatcg agttgacttt tgtgaataat attgcctccc caaagggaga tcaattcgcc
     1141 tttggaaata ggagcaatgg caaaaacggc acaaccggta acgcgcttgg ggcgagcttc
     1201 gcatttcggg tttaagtaag aggcagtcga tttgggttga gtaaacatgg tcaataggaa
     1261 gaatcaggtt cgatacggga aagaagcgga agccagaaag ccgtattgct ttctggcttt
     1321 atccaattat tttgagtcgc cttctgtatc cgcagaggaa gcagagatac gttgggcgaa
     1381 aaagatcggg agaaaggtaa ttagtaccac aatggttgcg acaacatttg tcacggggcg
     1441 gtcacgcgga cggacgagtt ggctgaaaat ccaaataggc aaagtagcct gttgcccagc
     1501 agtaaatgtt gtaacaatga cttcgtcgaa ggaaagtgca aacgcaagca tgccgcctgc
     1561 taccagcgct gtggcgatgt tcggcaagac gacatagcga aatgtctgga aagaatttgc
     1621 gcccaagtcc attgaggctt cgacttgcga accttgcatc cgacggaaac gcgctaacac
     1681 attgttgtaa accgtcacaa cacaaaaggt ggcatgccca attacgatgg tccaataaga
     1741 aaatgggatg tttagcgtgc caatggcgga acgcaaagca atacccgtca caatgccggg
     1801 caaggcaatt ggcaaaatta gcaacagcga gatggtttct ttgccgaaga agtcactgcg
     1861 gtacacagct gcggcggcaa gggtaccaag cactaatgca acaagagtag ccacaaccgc
     1921 tacttgcaga gaaagtgtga gcgcattcca caagtccacg cgctggagcg ctgcgccaaa
     1981 ccatttggtt gtgagtccgg gaagtggaaa tgtgtatgtg gcttcgtctg ttgtgaatga
     2041 gtacaggaag atcacccaaa ttggggcatg tagaaagagc aagccgccaa tggctgcaag
     2101 caccaaggcg ggagaagcag agtctgaaga cttagagcgc atcgaaagct cccaatttac
     2161 gggcgatcaa taaataaaca accataatca cgatgggacc catcgtgaac gcagcagcga
     2221 gtggaatgtt gccagccgta ccttgatagg aatacacagc ctgaccaata aaaaagcgcg
     2281 aattgcctag cacttgagga ataataaaat cacctaaagt tagggaaaat gtaaaaatag
     2341 aacccgcgac tacgccaggg aaggacaagg gtaacaatac ttggcgaaaa gtttgtgaag
     2401 ggttcgcgcc caaatcactg gatgcttcga gcaaagattt tggcacgcgt tccaatgctg
     2461 tttgaatggg gacaatcata tatggcagcc agatatagca aaatgcaatg aacatcccaa
     2521 ttggagaaag cgaaagagat gaaccgccaa taccgggaat ggaaagaatg ccgtccaaaa
     2581 taaattgcaa cccaaataaa ttaaagaacc aagaaacaat gccttctttg gcgaggatca
     2641 atttccatgc gtagacacgt acaagatagc tggaccaaag tggaatggtg acggcaatat
     2701 acaaataagt ctttaatttt ttcggcgcaa accgcgcaat ataatatgca agcggaaaag
     2761 caataatggc atctgcaatg gttactagca gcgccatggt tgccgtgcga aagaaaatac
     2821 ctaggttggt tgggtctaaa agtttggcgt atgtttgtaa tgtaaattct cgaacaacca
     2881 agcctgtaaa ttcttcaagg taaaagaagc tgttgatcaa taacgaaaac agagacccca
     2941 aatacaccac acccatataa agcatgggag gtccgagcaa aagccctagc acgaggcggg
     3001 gatgccggta caaataggtg gagatgcggt taagcacctt tgcctcccaa aatactaatg
     3061 tggtcttgtt gccatgaaag ccgcacggca ctgcccttag acgagaagac gtcggcagag
     3121 gttgttttca aattttgtga aatgaccaca aggtcgatgc cttgcgtgat ttcaaccaaa
     3181 tagcgagtgt acaagccaag ataaatcaca tcgcgaatgg tgccatccac ggcgtacata
     3241 tctgagctaa tgtattcctg ttgtgaacca atatgaatct tttcagggcg gatggaaaaa
     3301 gtctgcgcgt gacctgtgat gcgttcggcg acgtctccgc taatcaaatt ggatgtaccc
     3361 acaaaccccg ccacaaactg cgacgcggga tgttcataca cttcagacgg ggtaccaatc
     3421 tgttcaattt tcccctgact aaaaaccgca atgcgatcac tcatggtgag cgcctcttct
     3481 tgatcgtgcg ttacgaagat gaaggtaata cccacccgct tttgaatgga cttaagctcc
     3541 acctgcattt gctggcgtaa tttcaagtct aatgcgccga ggggttcatc gagtagcaaa
     3601 actttggggt gattgatcaa ggcacgggca agtgcgacgc gttggcgctg tccgcctgag
     3661 agttgggaag gtttgcgtcc ggcaaagcca gagagttgga cgagatccag catttcttcc
     3721 actcgtttca tacgctcggc tttttctacc tttt
//
LOCUS       DAOQNJ010000225         3741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_16333_length_3741_cov_8.196148, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000225 DAOQNJ010000000
VERSION     DAOQNJ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16333_length_3741_cov_8.196148"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLG52_07510"
     CDS             complement(<1..682)
                     /locus_tag="PLG52_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41331.1"
                     /translation="MSPRNSNLFLFALILSSAVLALLRFNSLQIGTSYDDAKYIILAE
                     SLASGEGYQLINFPRPQIERNFPPGWPLVLAPFTLVFPKNYDALKVVSLALWLLSILL
                     VYKLFSKRLSSPFLEILTALVALNPLLVGTSITLMSETAYLFFSLLALVVFDKADGKS
                     WGWLILATLLAFYTQQVRTIGMSLTISLLVILLLSKRFKEIAMVATLFIGGFAFQSWL
                     NLRNGGTVI"
     gene            complement(685..1224)
                     /locus_tag="PLG52_07515"
     CDS             complement(685..1224)
                     /locus_tag="PLG52_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB41332.1"
                     /translation="MDHTNHVNLLSPASLDKGGSWADLGAGSGAFTFALRELIGPMAT
                     IYAIDKDRNSLNSLKSEYRVRFASDENLHLQVEDFSRTSNLPPLDGILVANSLHYFKD
                     RVKVLQHIKSFLKPNGKLIVIEYNVDSGNLWVPYPFSFGTFGKLAAQAGFAEPQLLAK
                     NPSSFLREFYSALTFKAEV"
     gene            1362..2603
                     /locus_tag="PLG52_07520"
     CDS             1362..2603
                     /locus_tag="PLG52_07520"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB41333.1"
                     /translation="MENSTYHDPYAPLRFRDYRLLLSGRFISAFANEMLSFAIGWELW
                     LRTHSAFALGMVGLVQVVPVILLSLPAGHVADQYNRRRIVIISEVLIALCVITLGWLS
                     YTEGSLVLIYIALLGIGIARAFNEPASSTLTPETVPPELFSQAATWGSGIWHFASITG
                     PALAGLLVGFFHSVTFIYVYAALSAITYCVLISLIKGRQLPLAEKSATLDSVREGLRF
                     MWNTKVILAAITLDMFAVLFGGAVALLPIYATDILHVGAQGLGIMRAAPSLGALAMAF
                     LIAHLPPMKNAGKTLLFAVTGFGLATIVFGLSTNFFLSVTMLMLLGGFDNISVVIRST
                     LMLTQVPDEMRGRTSAVNNIFIGVSNELGAFESGFVAALVGPVITVVGGGIGTILVVL
                     AIAGIFPAMLKLKSLDVPKPS"
     gene            2663..>3741
                     /locus_tag="PLG52_07525"
     CDS             2663..>3741
                     /locus_tag="PLG52_07525"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNB41334.1"
                     /translation="MMNNKQLAAVFTLIANLLEIKGEIIYKTLAYRKASESLDTLGRE
                     ASEYWKEGKLADIPGVGKAIAEKIDELLNTGKLEFLEKLKKEVPESLAGWLQVPSLGP
                     KKIAMIWKALNITTLAELEAAAKAGKLKDLPGMGAKSETAILEGIASLARRSGRLPLG
                     KAYPLAQEIIKVLKGVKGVVDVQPAGSLRRMRSTVGDLDILVAAKDSAAVMEAFVNLK
                     GVERVLGKGDTKASIEFFDGVRAQVWVHPPEKFGTALQYATGSKDHNVKLRQIALDKG
                     LSLSEHALTKVKGKGEILCATEEEVYKTLGLQYVPPEMREDRGEVEAAKANKLPKLIQ
                     VKDIKADLHMHSTYSDGKLSMLDMAR"
BASE COUNT          943 a          832 c         1018 g          948 t
ORIGIN      
        1 agataacggt cccgccgttg cggaggttga gccaactctg gaaggcgaat ccacctatga
       61 aaagagtagc gaccatggcg atttctttga agcgtttgga aagtaataag ataacgagta
      121 atgatatggt gagtgacatt ccgattgtgc ggacttgttg tgtataaaat gcgaggagtg
      181 tagcaaggat aagccagccc catgattttc catccgcttt atcaaagacc accaatgcca
      241 gtaatgaaaa aaacaaataa gctgtttcag acatcaatgt gatcgaggtg ccaaccaaaa
      301 gtggattcag cgccaccagt gcagtgagga tttcaaggaa gggggaactc agccgttttg
      361 aaaatagttt atagaccaac agaatggaca agagccaaag tgccagcgaa acgaccttga
      421 gtgcatcata atttttggga aagaccaagg tgaacggcgc aagcacaagc ggccagcccg
      481 gcgggaaatt ccgctcgatc tgcggacgcg gaaagttgat gagttgatat ccctctccgc
      541 tggcaaggct ctcggcgagg atgatgtatt tggcatcatc gtaggaggtg ccgatctgaa
      601 gcgagttgaa acgtagaagc gcgagcacgg cgctgctgag aatcaatgcg aacaaaaaga
      661 gatttgaatt tcttggtgac atgctcatac ctctgccttg aacgtcagcg cggaatagaa
      721 ctcgcggaga aaacttgaag ggtttttcgc aagcaactgt ggttcggcga agcctgcctg
      781 tgctgctaat tttccaaacg ttccaaatga gaatgggtag gggacccaaa gattgcccga
      841 gtccacgttg tattctatga cgataagttt gccattcggc ttgagaaaag acttgatgtg
      901 ctgtaagacc ttaaccctat ctttaaaata atggagagag ttggcaacaa ggatgccgtc
      961 aagagggggg agattggatg ttcgcgaaaa gtcttctact tgcaggtgca gattttcatc
     1021 gcttgcaaag cgtacacgat attcgctttt taatgagttc aggctgttac gatctttgtc
     1081 tatggcgtag atcgtagcca taggaccgat cagttcacgc aaggcaaagg taaaagcgcc
     1141 agaacccgct ccgaggtccg cccatgagcc gcctttatcc aaagaagctg ggcttaatag
     1201 gttaacatgg tttgtgtgat ccataagtga atcgtatcat acccatgatc ctgagggttg
     1261 tcgtgaaatt taagactccc ttttcaaatc ttatgaaatg gactaagtct ttaagtttac
     1321 ggatggtatc atacacacca tctccgaaaa taggatttga aatggaaaac tctacatacc
     1381 acgatcccta tgcgccgctg cgttttagag attatcggtt gctgctcagt gggcgtttta
     1441 tcagcgcatt tgccaatgag atgctgtcct ttgccattgg gtgggaactc tggctgcgta
     1501 cccatagtgc ctttgccttg ggcatggtgg ggttggtgca ggtggtgccg gtgattctgt
     1561 tatctctgcc agccgggcat gtggcagatc aatacaatcg tcgtcgcatt gtcatcatta
     1621 gtgaagtttt gatcgccttg tgtgtgatca ctttgggctg gctttcctat actgaagggt
     1681 cgttggtgtt gatctacata gccttattgg ggatcgggat tgccagggca tttaacgaac
     1741 cggcttcttc aaccctgacg ccagagacgg tgccaccaga gttgttttca caagctgcca
     1801 catggggcag tggaatctgg cacttcgcct cgatcaccgg accggcactg gctggcttgc
     1861 tcgtgggctt tttccactct gtgaccttta tctatgttta cgcggcgcta tcggctataa
     1921 cgtactgcgt gctcatttcg ttgatcaaag gcaggcaatt gccgttggca gaaaaatctg
     1981 ccacgctgga ttctgtccgt gaaggtttgc gctttatgtg gaacacgaaa gtgatcctgg
     2041 ctgcgatcac actggatatg ttcgccgttc tctttggtgg agctgtagcg ttactgccga
     2101 tctatgctac ggatattctg cacgttggcg cgcaaggatt gggaatcatg cgcgctgcac
     2161 catcgttggg cgccctggct atggcatttc tcattgccca tttaccgcct atgaagaatg
     2221 caggcaagac cttactattc gctgtgactg ggtttggctt ggcgaccatt gtctttggtc
     2281 tttcgaccaa cttttttctc tcggttacca tgcttatgct gcttggcggc tttgataaca
     2341 ttagtgttgt gatacgcagc accttgatgt tgacccaggt gcccgatgaa atgcgcgggc
     2401 gcacctccgc agtgaataat atcttcatcg gcgtatcgaa tgaactgggc gcttttgagt
     2461 cgggctttgt cgcggcatta gtaggacctg tcattacagt agtaggcgga ggtattggga
     2521 ccattctggt agtgctggca attgccggca tcttcccggc gatgctaaaa ctaaaatcgt
     2581 tggatgtgcc aaagccatca taaatctgta caaaggtatt gatatccatg ttgacttata
     2641 attgacctat tagaggtgcc tcatgatgaa taacaaacaa ttagccgctg tctttactct
     2701 gatcgccaat ttactggaga tcaaaggcga gatcatttat aagacgcttg cctatcgcaa
     2761 agcatcagag agtctggata cgcttgggcg tgaagccagt gagtattgga aagagggaaa
     2821 attagccgac atccccggtg tgggcaaagc catcgccgaa aaaattgacg aactgctcaa
     2881 cacgggcaaa cttgaatttc ttgagaagtt gaaaaaagaa gtgccagaga gtttggcggg
     2941 ctggttgcaa gtcccttcgc tgggacccaa gaagatcgcc atgatctgga aagcgttgaa
     3001 catcaccacg cttgctgaac tcgaagcggc ggcgaaagcg ggcaaactaa aagacttgcc
     3061 cggcatggga gccaaatcag aaaccgcgat tctcgaaggc attgcatcgt tggcgcgccg
     3121 ttcgggtcgt ttgccgttgg gcaaggcgta tccgctggcg caggagatca tcaaagttct
     3181 caaaggcgta aaaggtgttg tcgatgtgca acccgctggc agtttaagaa ggatgcgttc
     3241 gacggttggc gatctggata tcctcgttgc ggctaaagat tcagcggctg tgatggaagc
     3301 cttcgtcaac ctcaaaggtg tggaacgtgt gttgggcaaa ggtgatacca aagccagcat
     3361 tgaatttttt gacggcgtgc gtgcgcaagt ctgggttcac ccgccagaga agtttggcac
     3421 ggcgttgcaa tatgccacgg gctcaaaaga ccataacgtc aaactgcgtc agatcgcgct
     3481 ggataaggga ctgtcgcttt ctgaacatgc gctcactaaa gttaaaggca agggcgagat
     3541 tctttgcgcc accgaagagg aagtgtataa gacgctcggc ttgcaatacg tcccgccgga
     3601 gatgcgtgaa gatcgcggcg aagtggaagc ggcgaaggcg aataaacttc ccaagttgat
     3661 tcaggtcaaa gacatcaaag ccgatctgca tatgcattca acctatagcg atggcaaact
     3721 ctccatgctt gatatggcgc g
//
LOCUS       DAOQNJ010000226         3713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_16526_length_3713_cov_9.022143, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000226 DAOQNJ010000000
VERSION     DAOQNJ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16526_length_3713_cov_9.022143"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /gene="rlmN"
                     /locus_tag="PLG52_07530"
     CDS             <1..590
                     /gene="rlmN"
                     /locus_tag="PLG52_07530"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNB41335.1"
                     /translation="EVVSNVVFMGMGEPFHNYDNVMSAIDRLNDADGYKFGARRLTIS
                     TVGLVPQIRRFADEQRQVNLAISLHAADDDERLAIMPVNKRYKIDDVIDACKYYIEKT
                     HRRVTFEWALINGVNDTPEVAQKLASRLKGLLCHVNAIPLNPTQGYQGEATSRQRSQV
                     FKETLEQAGIPCTIRMRRGIDINAGCGQLAGAESK"
     gene            762..1418
                     /locus_tag="PLG52_07535"
     CDS             762..1418
                     /locus_tag="PLG52_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB41336.1"
                     /translation="MKTTTLLVIEAKHAEVPSFAADLQKKGFDVHIAQNGSKAVALLK
                     EVSPSLVVVNAASMRSTGLRICQSLREKDSKLPIILILDEEKEVSKDAADAVLILPFT
                     VQKLSNRIKPLLPGDGKNVLHVGPIRLDLEKRRVRCLGKSTKLTPRLVTLLQLLMEKH
                     GEVVEREPLFKKAWETNYTGDTRTLDVHISWLRRAIELDPDNPKFLKTVRGVGYRLDI
                     "
     gene            complement(1407..2630)
                     /locus_tag="PLG52_07540"
     CDS             complement(1407..2630)
                     /locus_tag="PLG52_07540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNB41337.1"
                     /translation="MPTLHWNGKHLSQPQPASLTLDSVLHPKGRGYPKSRTDGRVIQG
                     DNLAVMAALLPEYEGRINLIYADPPFFTNRQFHARVGRGEDSRKPSKWKLAEGYQDSW
                     TDMDSYLQFLYERLSLMHRLLAKNGTLYLHLDWHADSHARLILDEIFGAENFINEIIW
                     AYHGPSPIRTAFNRKHDTILMYAKSKEYIFNVDDVREPYNPNTVATFKASRKAGFGKI
                     PDLERGKVPEDWWYFPVVARLHNERTGYPTQKPEALLERIILASSNKNDLVADFFCGS
                     GTTALVAAKHGRKFITSDQSARAVHTARARLAETKSVFSMERDVMVKTEIATKSKKIK
                     VKLMGDRVLLQTSLDVDFWEVDPAWDGKIFRSVAQAQRHVRSGEIPMELKIKLGGKVC
                     IRVVTVEGKQFQLNI"
     gene            complement(2689..>3713)
                     /locus_tag="PLG52_07545"
     CDS             complement(2689..>3713)
                     /locus_tag="PLG52_07545"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNB41338.1"
                     /translation="LAFTPFLPGLTDFDDSFVVRIGTKDMPVRKEPTDKSRIELSHYR
                     DELVHVYGEITAEEPKHNPVWYRVWGGYLHRGRLHRVRTIYQTPIDSIPEGTRLIADV
                     SVPFTTPYRYSKTYGWQQMSPPLYYGSVHFIEAVEQGPEMADYKGPWYRIFDELDSNV
                     TYYAPAIHMRILPQDMLTPISPDVPYEDKLIEINLTSQMLYAYEYGSLVFQTNISSGV
                     PGDPTGGTGVPTTTPTGKFTILDKVPAKHMGYSYFGAQTTGNVLADVDNYVLPGVPWT
                     SFFTSQGHAFHGTYWHENFGSPMSHGCINMRTNEANWIFRWARPEPIVGNVATRGLGT
                     TVEIHY"
BASE COUNT          998 a          850 c          916 g          949 t
ORIGIN      
        1 acgaagttgt aagcaacgtc gtttttatgg gcatgggcga gccttttcac aactacgata
       61 atgtgatgtc tgccattgac cgtttgaacg atgccgatgg atacaaattc ggggcacgcc
      121 gcttgaccat ttccacagta gggcttgtgc cacaaattcg gcgctttgca gatgaacagc
      181 gccaagtcaa cttagccatt tcccttcacg ccgccgatga tgatgagcgt ctcgcgatta
      241 tgccggtcaa caaacgttat aagattgatg atgttatcga cgcctgcaaa tactacattg
      301 aaaaaacgca tcgtcgcgtg acctttgaat gggcgctcat taatggagtc aacgatacgc
      361 cggaagtggc gcaaaaactc gcctccagac tcaaaggctt gctttgtcat gtgaatgcca
      421 ttccattaaa ccccacacaa ggctatcaag gcgaagcaac aagccgtcag cgttcgcaag
      481 tattcaaaga aacattggaa caggctggaa ttccatgcac gatccgcatg cgccgcggta
      541 ttgatatcaa tgcgggctgt ggtcaacttg caggcgctga gtcaaaataa aattttctga
      601 tttgattcca caacttaaaa aatatgtcaa tgaaattaag atgcaaatag tactcaggtt
      661 tgagaccctt tgttaagata tggtgtataa tgaatcaatc ttaacaagct taaaagccta
      721 ttgcagtacg aattcataaa aaggcattat cgaaatttct tatgaagacc acaaccctat
      781 tggtcattga agccaaacac gcagaagtcc cttctttcgc agcagacttg cagaagaagg
      841 gatttgatgt acatatcgca cagaatggca gcaaagctgt tgcgttgttg aaagaagtca
      901 gcccaagttt ggttgttgtc aatgcagcat ccatgcgaag tactggttta cgcatctgtc
      961 agtcgctgcg cgaaaaagat tcaaagctcc ccattattct tattctggat gaagaaaaag
     1021 aagttagcaa agatgcagcc gatgcagttt tgattttgcc gttcacagtt caaaaacttt
     1081 ccaatcgcat caagccttta ttgcccggtg acggaaaaaa cgttttgcat gtgggtccta
     1141 ttcggttaga ccttgaaaaa cgacgtgtgc gctgtttagg taagagcaca aaactcaccc
     1201 cgcgtttggt caccttgctt caattgttga tggaaaaaca tggagaagtg gtggaacgtg
     1261 aaccgctctt caaaaaagca tgggaaacca attacaccgg tgatacccgc acccttgatg
     1321 tacatatctc atggttgcga cgcgccattg agctggaccc cgataaccca aaatttctca
     1381 agaccgtgcg cggtgttggg tatcgcttag atatttagtt gaaattgctt tccttcaacc
     1441 gttacgactc ggatgcagac tttgcctccg agttttattt ttaactccat cggaatttcc
     1501 ccgcttcgca cgtgacgctg agcctgtgct acgctgcgga aaattttccc atcccaagct
     1561 ggatccactt cccagaagtc cacatccaaa gatgtttgca aaaggactcg gtctcccatc
     1621 aacttgacct tgatcttctt tgatttggtc gcaatttctg ttttgaccat cacatcacgt
     1681 tccatggaga acacagactt ggtctcagcc aatcgtgcgc gcgcggtatg cacagctcga
     1741 gcagactggt cggaggtaat aaatttgcgt ccatgcttgg ctgccacaag agcggtggta
     1801 ccagaaccac agaaaaagtc tgctactagg tcatttttat tcgaagatgc caaaataatg
     1861 cgttcgagca aagcttctgg cttttgagtg ggataccccg tgcgttcatt gtgcaggcgt
     1921 gccaccacgg ggaaatacca ccaatcttct ggcactttgc cacgttcaag atcaggaatc
     1981 ttgccaaagc ctgccttgcg agatgctttg aaggttgcta cagtattagg gttatacggt
     2041 tcacggacat catctacatt gaaaatatat tctttgcttt tggcatacat caagatggtg
     2101 tcgtgttttc gattaaatgc ggttcgtatt ggagaaggtc catgatatgc ccagataatt
     2161 tcattgatga agttctctgc gccaaaaatt tcgtcgagga tgaggcgggc atgggaatca
     2221 gcgtgccagt caaggtgaag atataacgtg ccgtttttgg cgagcagtcg atgcatgaga
     2281 ctgagacgtt cgtacagaaa ttgcaggtac gagtccatat ctgtccatga atcttgatag
     2341 ccttcagcaa gtttccactt ggaaggtttg cgagagtctt ctcccctccc cacacgggca
     2401 tggaattgtc tgttagtgaa aaatggtggg tcggcgtaaa tgaggttgat acgcccttca
     2461 tattcaggca acaaagcagc catcacagcc aagttatccc cttgaatgac tcgtccatcg
     2521 gtgcgcgatt tggggtagcc acgtcctttc gggtggagga cagagtcaag ggttaatgaa
     2581 gcaggttgcg gctgggaaag atgcttcccg ttccagtgta aggtaggcat gagagcaggg
     2641 aaacctcaaa tggtggattc ggtttgggag aagaaatccc aaaccgtttt agtaatgaat
     2701 ttcaacagta gtgccaagcc cgcgagtggc gacattgccc acaatcggtt ctgggcgtgc
     2761 ccagcggaag atccaattgg cttcgttggt gcgcatgttg atacagccat ggctcatagg
     2821 cgagccaaaa ttttcatgcc aataggtgcc atggaaggcg tgaccttgcg aagtgaagaa
     2881 tgaagtccac ggaacgccgg ggaggacgta gttatctaca tcggcaagga cgttacccgt
     2941 tgtttgcgcg ccgaagtaac tataccccat atgcttggca ggcactttat ccaaaattgt
     3001 aaatttgcca gtcggtgttg tagtgggcac acctgtgccg cccgtagggt cgccaggaac
     3061 acccgaagaa atattggttt ggaagaccaa actgccgtat tcataagcat ataacatttg
     3121 cgatgtgagg ttgatctcaa tcaatttatc ttcgtatggc acatcgggag aaataggcgt
     3181 caacatatcc tgcgggagga tgcgcatatg aatggcaggt gcataatagg tgacgtttga
     3241 gtcgagttcg tcgaaaatgc gataccaagg acctttgtaa tctgccatct cgggtccctg
     3301 ttctacggct tcaataaagt gaaccgagcc ataatacaac ggcggagaca tttgctgcca
     3361 accataggtc ttggaatagc gatagggtgt ggtaaatggc acagaaacat ctgcgatcag
     3421 acgtgtacct tcagggatgg agtctatcgg ggtctggtag atcgtgcgca cgcggtgcaa
     3481 acgtccgcgg tggaggtagc cgccccacac ccgataccaa accgggttgt gtttcggttc
     3541 ttcagcagtg atctcgccat acacatgcac caactcatcg cgataatgag aaagctcaat
     3601 ccggctttta tcggttggtt ctttacgcac gggcatatct ttggtgccaa tacgcacaac
     3661 aaaactatcg tcaaaatcgg taagaccggg caaaaatggc gtaaaagcca gcc
//
LOCUS       DAOQNJ010000227         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_16552_length_3710_cov_10.131874, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000227 DAOQNJ010000000
VERSION     DAOQNJ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16552_length_3710_cov_10.131874"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLG52_07550"
     CDS             <1..509
                     /locus_tag="PLG52_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNB41339.1"
                     /translation="GHRLGFHHYEKKAKWSLPTKISVAALVVLAIQISYGGMTAGLKA
                     GHVSDTWPLMFGKLIPPNLFNSFINILETPQTIVFIHRWFAWLGVILVPVVFYIIKKQ
                     NYPADIQKGLLWLLGVVALQITLGVLTILSYVNIVIALLHQANAILLLGLAVYFIHRF
                     RAVDEAKA"
     gene            complement(554..790)
                     /locus_tag="PLG52_07555"
     CDS             complement(554..790)
                     /locus_tag="PLG52_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41340.1"
                     /translation="MMIKNRFYMFLGLLLLLLGVVLPFLMIIKVVEPTFFLVFLTSGS
                     STVGLALGMFGLTRWSVTAIKKRPPQIEDWRQTE"
     gene            complement(787..1818)
                     /locus_tag="PLG52_07560"
     CDS             complement(787..1818)
                     /locus_tag="PLG52_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41341.1"
                     /translation="MISELQEAKRMSVKIDEPTETLLAIHNLHVWYELRRFGFSVVGH
                     VKAVNNVSFELGKGETVAVVGESGCGKSSLMKTILGLNVPTGGELLFEGKRLTALTEA
                     ELRSYRFEIGYVQQDPYGALPPFMNVEQILEEPLIISGVSSKDERRERIHKILGEVKL
                     HPVEDFLHKYPHMLSGGQQQRVVIARAMVLEPKMIVADEPVSMLDASVRVEILKLMHA
                     LQEQHHLSVIYITHDLSTVKYFSARIFVMYAGSLIEKAETRQLLKNPLHPYTHALLAA
                     TSDPNAANAEVYKAVPPGEPPSLADPPKGCRFHPRCAKAMTGVCDVKEPPDFEPEPNH
                     FVACWLYEA"
     gene            complement(1835..2413)
                     /locus_tag="PLG52_07565"
     CDS             complement(1835..2413)
                     /locus_tag="PLG52_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB41342.1"
                     /translation="MPSLALYDVLYSFKDIHKVVLEFASELNEEQLRWRPRGYSTSIG
                     FHLWHLARESDFLKAIILERTPQLGSDFGEPKEIWAVEKLAERWRFPTDLNATVGTGL
                     SDEIAATLPIPPKDELVDYLQKSYEALETFIALLDERYPTFEGVEEDLQRKLKNIRLN
                     LLVFLSHDCRHLGMMECLKGLQTGFGSATEKR"
     gene            complement(2431..3390)
                     /locus_tag="PLG52_07570"
     CDS             complement(2431..3390)
                     /locus_tag="PLG52_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41343.1"
                     /translation="MTELTPLLEVKDLKLHFRTQKGVVQAVDGVDLDLGFHRAVVILG
                     ESGCGKTSLSKAMLRLLPRNVAKYSGELVINGKDVMPFTDEEYRQNVRWVSMSLVPQA
                     AMNALNPVITVGEQVSEPAMLHLGLNKDEALGMAYKMFQHVGIPADFISRYPFELSGG
                     MRQRVALAMALVTSPNLIILDEPTSALDLLTQANIMNVLKRIKHELGTSYILITHDIA
                     TSSELADEVAIMYAGQIVETGDAKDFFPAPLHPYSQMLMASVPRLRSESDPMFITGQP
                     PSLMNPPTGCRFAARCPFRFEKCDQEPPVFQKGNRKVKCWLHQ"
     gene            complement(3404..>3710)
                     /locus_tag="PLG52_07575"
     CDS             complement(3404..>3710)
                     /locus_tag="PLG52_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB41344.1"
                     /translation="NWRIIFHYLIPRMIPLLIPGLVQAVPAFVFLEASLAVIGLGDPV
                     LPTWGKIIQDAQANGALYKGYYYWVLEPAVLLIITGLAFAFLGFVLDRIFNPRLRDV"
BASE COUNT         1006 a          957 c          810 g          937 t
ORIGIN      
        1 ccggtcaccg acttgggttc catcactacg agaaaaaagc caaatggtct ttacctacga
       61 agatctctgt cgccgcactt gtggtgcttg ccattcagat cagctacggc ggcatgacag
      121 ccggtctcaa agctgggcac gtctcagaca cttggcccct catgttcggg aagcttattc
      181 cacccaacct atttaattcc ttcatcaata ttcttgaaac cccgcaaacc attgtcttta
      241 ttcatcgctg gttcgcttgg ttgggcgtca ttttggtgcc ggtagtgttc tacatcatca
      301 agaaacagaa ttacccagcc gatattcaaa agggtctgct ctggctactc ggagtggttg
      361 ccttacagat cacgcttggt gttctaacca ttctttcata cgtcaacatc gtcattgcct
      421 tgttgcatca agccaatgca attctgcttt tagggctggc tgtctacttc atccaccgct
      481 tccgcgccgt cgatgaagcc aaagcataaa tactttttgt aaaaatgcct cgccgtaaat
      541 ggcgaggcat tttttattcc gtttgccgcc agtcttctat ctgtggtggt ctctttttga
      601 tagccgtaac agaccaacgc gttaacccaa acatgccaag cgccaagcca acggtagatg
      661 agccagaggt caagaaaacg agaaaaaacg ttggttcgac cacttttata atcatcagaa
      721 acgggagcac aacacccaac agaagcaaaa gcaagcccaa gaacatgtag aagcggtttt
      781 ttatcatcat gcctcatata accaacaagc cacaaaatga ttcggctcgg gttcaaaatc
      841 tggtggttct ttcacatcac aaacacctgt catcgcttta gcacaacgcg gatgaaagcg
      901 acatcccttg ggcgggtctg ccaaactggg cggctcgccg ggcggaacgg ctttatacac
      961 ctcagcattg gcagcattgg ggtccgaggt agcggcaagc aaggcatgag tataggggtg
     1021 caatggattt tttagcaatt ggcgcgtctc tgctttttca atgaggctgc ctgcatacat
     1081 cacaaaaata cgcgctgaaa aatacttcac tgtagaaagg tcatgagtaa tgtaaatcac
     1141 tgaaaggtga tgttgctctt gcaaagcatg catgagcttc aagatttcca cgcgcaccga
     1201 agcatctaac atggagacgg gttcatctgc cacaatcatc tttggttcca acaccatggc
     1261 tcgcgcaatc accacgcgtt gctgctgccc gccgctcaac atatgtggat acttgtgcag
     1321 gaaatcttcg acagggtgca gctttacttc tcccaatatc ttatgaatac gctcacgccg
     1381 ttcgtcctta cttgaaactc cactgatgat gagtggctct tccaagatct gctcaacatt
     1441 cataaatgga ggcaaagcgc cgtaggggtc ttgctgcaca tatccaattt caaagcgata
     1501 gctgcgcaat tcagcttctg tcaatgcggt gagacgtttc ccttcaaaaa gaagctcacc
     1561 gcccgttggc acatttagcc ccagaatggt cttcatcagc gatgacttac cgcatccact
     1621 ctccccgact actgctacgg tttccccttt acccaactca aagctgacgt tatttacggc
     1681 tttgacatgc ccaaccacgc taaagccaaa gcggcgaagc tcataccaaa catgtaaatt
     1741 gtgaatcgca agcaacgtct ctgtcggttc atctattttc acagacatgc gttttgcctc
     1801 ttgtaattct gagatcatag ttgtttatcg aatttcatcg cttctcagta gcagaaccaa
     1861 accccgtctg caaacccttc aaacactcca tcattccaag atgacgacaa tcgtgagaaa
     1921 ggaaaacaag caagttcaag cgaatatttt tcaattttcg ttgaaggtct tcttctacgc
     1981 cttcaaaggt tggataacgt tcatctaaca aggcgataaa tgtctctaat gcttcatagg
     2041 acttctgcag ataatctacc aattcgtctt taggcggtat gggcaaagtc gctgcaattt
     2101 catcacttaa cccggttcca actgtagcat tgaggtcggt aggaaaacgc cagcgttctg
     2161 ccaatttctc aacagcccaa atctctttgg gctcgccaaa gtcactgccg agttgcgggg
     2221 tacgttctaa aatgatggct ttcaaaaaat cagattcgcg ggctaagtgc caaagatgaa
     2281 acccaataga tgtactatac ccacgcggac gccaccgtag ttgttcttcg ttcaattcag
     2341 atgcaaactc aagcacgacc ttatgaatat ctttgaaaga ataaagcacg tcatacaatg
     2401 caagggacgg catggatgaa actccttggt ttattggtgc agccagcact ttactttacg
     2461 gtttcctttt tggaaaaccg gcggctcttg atcgcatttc tcaaaacgga acggacagcg
     2521 cgccgcaaag cgacaccccg ttggcgggtt catcaaactg ggcggctgcc ctgtaataaa
     2581 catcgggtct gattcgctgc gtaagcgtgg aacgctcgcc attaacattt gcgaataagg
     2641 atgcagtggt gcagggaaga aatctttggc atcgcccgtt tccacgatct gaccggcata
     2701 cataattgct acttcatctg ctagctcgct ggaggtggcg atatcgtgtg tgatgagaat
     2761 gtacgaggta cccaattcat gcttaatacg tttgagcaca ttcataatat tggcttgggt
     2821 cagcaaatcc aaagcagaag tgggttcgtc caaaatgatc agatttggcg aagtgacgag
     2881 tgccattgcc aaagcaaccc gttgccgcat accgccactc aactcgaatg gatagcgact
     2941 aatgaagtca gcagggatcc ccacatgctg aaacattttg tacgccatgc caagcgcctc
     3001 atctttattc aaaccaagat gcaacatagc tggctctgaa acctgttctc ccactgtaat
     3061 gaccgggttc aacgcattca tagccgcttg aggtacaaga gacatactca cccagcgcac
     3121 attctgccgg tattcttcat cggtaaacgg catcacgtct tttccattaa tgaccagttc
     3181 gccgctatat ttcgccacat ttctaggcag caagcgcaac atggcttttg aaagcgatgt
     3241 tttaccgcac cccgattcac ccagaatgac tacagcgcgg tgaaacccca agtcgaggtc
     3301 tacaccgtca acggcttgca caactccttt ttgggtacgg aagtggagct tcaggtcttt
     3361 tacttcaagc aagggtgtca actctgtcat tgatacgtct cggttacacg tcgcgcaggc
     3421 gcgggttgaa gatgcgatct aatacaaagc cgaggaaagc aaaagccaac ccagtgataa
     3481 tcaacaaaac agcaggttcc aacacccagt agtaatatcc cttgtataaa gctccattcg
     3541 cctgagcatc ttgaataatc ttgccccaag tcggtaagac cgggtctcct aaaccgataa
     3601 cagcaagaga cgcttcaagg aacacaaagg ctggcacggc ttgaaccaaa cccggtatca
     3661 acaaggggat catgcgtgga atgaggtaat gaaaaataat acgccaattt
//
LOCUS       DAOQNJ010000228         3708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_16564_length_3708_cov_10.538188, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000228 DAOQNJ010000000
VERSION     DAOQNJ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16564_length_3708_cov_10.538188"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLG52_07580"
     CDS             complement(<1..313)
                     /locus_tag="PLG52_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNB41345.1"
                     /translation="MKTQPLQPFRVADESGKENASPLFWAGVALLMVVAFFFVFFTYN
                     SGKKPTPSEYSASDIQTRQSVSLSQYRGSAIVLMSWATWCMDCEEELTALEQLWKSEL
                     GR"
     gene            complement(319..1275)
                     /locus_tag="PLG52_07585"
     CDS             complement(319..1275)
                     /locus_tag="PLG52_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41346.1"
                     /translation="MKNTLLTFYIIFLVIAAGFGVGAATEAGVLIPAAVPFALILIAL
                     LYFLKPQAELLGWAALTVGLLAGTYLVTGEVIEYVIFIIYLILTILGIFKSPYFLAFA
                     WLFHPIWDFLPRELPGHMHDLPLACAFFDTPIGLYLLWGSWKKRWLPFGGDTSNKAAF
                     LRSGKSIFIGVLIMAVSSAIVAAIGSGYLNWVALAASLIIIFGFRFMGETAELIAWAI
                     LTGWLGMTYAHTGGMGDALFFFAYVAISALGVFKSPYLLGLAWLVFIPYSFLPHHAHH
                     MSPDFALATIFYCVPAGLYLLRGAWKNRWQAFDNSPASPAVS"
     gene            complement(1364..1879)
                     /locus_tag="PLG52_07590"
     CDS             complement(1364..1879)
                     /locus_tag="PLG52_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNB41347.1"
                     /translation="MKKIFAFILAGALLLSACGGGMTGGDEHTDGEGVEAHDYWARAA
                     MAGGNSAAYMLLHNHGTEDDALISASSDVADAVELHLSQMKADGTMEMIQQEKIALPA
                     DGEVELKPGSYHVMLIGLKQDLMAGSEITITLTFEHAGEITLTVPVKDAADMGGESMN
                     MGGMDMATPTP"
     gene            complement(1911..2480)
                     /locus_tag="PLG52_07595"
     CDS             complement(1911..2480)
                     /locus_tag="PLG52_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HNB41348.1"
                     /translation="MSPNSLNREQQILDHLQKHGNASIQELVEVLGVSNMTIHRDLNK
                     LAEAGRVIKKHGGVRLPSTSRQTEDSSYCAMCSKPVSQRTVFIVKLENGDELRACCSH
                     CGLMLQSRSESVWQSLTADYLYGHMISAGQAIYLVGSEVSICCVPSVLVFGSRVEAEK
                     FQKGFGGQLLGMDETIQHMRGMMHMHSGM"
     gene            complement(2561..>3708)
                     /locus_tag="PLG52_07600"
     CDS             complement(2561..>3708)
                     /locus_tag="PLG52_07600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB41349.1"
                     /translation="STILFLLALIIIYWIHNQYHLDIVVTPVPAPTSAPKVSVCIPAR
                     NEETNIRRCVEAVLRQDYPNFEIIVLDDRSTDSTLTLLKEIASRDSRLLPISGSDLPE
                     GWAGKPHALYQASAVAKGEWLCFVDADTFLEPNALSSVYAKALETKADLFTMMTRQIL
                     GSFWELTVMPLVMTALSVGFSPRKVNDPARRDAVANGQFIFIKRSIYDLIGGHEKIKD
                     QIVEDKALSENVKWNGHRLVVADGRLVASTRMYTSLETMWEGWTKNIYLGLRDHPSLL
                     LLGAFGATLALFAALFLPVWPLLGLAWYFNHGGWMAWAVIVEALLVWGYLIYFRFMVA
                     KEMEIPRWYAWTTPLGAGVFAAMMLTSAWKVLTGQGVTWRGRKYHSK"
BASE COUNT         1045 a          986 c          827 g          850 t
ORIGIN      
        1 cccgtcccag ttccgatttc cagagctgct caagtgctgt gagttcttcc tcgcagtcca
       61 tgcaccaggt tgcccaactc attaacacaa tcgcagagcc gcgatactgg ctcagtgaaa
      121 ccgactgtcg tgtttgaata tcgctggcag agtattctga tggcgtgggt ttctttccgc
      181 tgttgtaggt aaaaaaaaca aaaaagaagg caaccaccat cagaagcgca accccagccc
      241 aaaacaaggg agaggcgttt tcttttccgc tttcatccgc cacacgaaag ggctgtaatg
      301 gttgcgtctt cactttactc agctcactgc aggtgatgca ggagagttat caaaagcctg
      361 ccagcgattc ttccatgcac ccctcagaag atacaaccca gcgggcacgc aataaaagat
      421 cgttgccagc gcaaaatctg gagacatgtg atgcgcgtga tgaggcagga agctatatgg
      481 aatgaacacc agccaggcta gccccaataa ataaggtgac ttaaagacgc ctaatgcaga
      541 aatggcaaca tacgcaaaga agaacaaggc atcgcccatg ccgccggtgt gagcataggt
      601 cattcccagc cagccggtaa ggattgccca tgcgatcaat tctgcggttt cacccataaa
      661 gcgaaaccca aagataataa taagagaagc tgccaatgcc acccagttca aatatcccga
      721 gccaattgcc gcaacaatcg cagaactaac cgccatgatc agaacgccaa taaaaattga
      781 cttgccgcta cggaggaacg cggctttgtt ggaagtgtca ccgccaaacg gcagccagcg
      841 ttttttccat gaaccccata gtaaatacaa accgatgggg gtatcaaaga aagcacaagc
      901 caggggcagg tcatgcatgt gacctggcaa ctcacgcggc aggaaatccc atatgggatg
      961 gaagagccag gcaaaggcaa ggaaataggg tgatttgaaa attcccagga tggttaatat
     1021 caaatagata ataaagatca catattcgat cacctcgcca gtgaccaaat atgtgcctgc
     1081 caaaagccca acggtcaacg cagcccagcc taacagttct gcctgcggtt tcaaaaaata
     1141 caaaagggca atcaagatca atgcaaaggg aaccgcagca ggaatcaata cccctgcttc
     1201 tgtggctgct cccactccaa aaccagccgc aatcacgaga aaaataatgt aaaacgtcaa
     1261 cagagtgttt ttcattttca aatccttcgg ctaaaaatca agttccccgc cttgcgggcg
     1321 gggaactggg agaatgcgtc tggcgaagac gcgtattcaa gaattaaggg gtcggagtag
     1381 ccatatccat tccgcccatg ttcatgctct ctccgcccat atctgcggcg tccttcaccg
     1441 gcacggtcaa tgtaatttca ccggcatgtt caaaggttag ggtaatggtg atctcactgc
     1501 cagccatcag gtcttgcttg agcccgatca acatgacatg gtagctgcca ggtttcaatt
     1561 ccacttcacc atcggcaggc aaagcaatct tttcctgctg gatcatttcc atggtgccat
     1621 cggctttcat ctgacttaag tgcaactcaa cagcatcggc tacatcagaa ctggcactga
     1681 tgagagcatc atcttctgtg ccatggttgt ggagaagcat ataagcagca ctgttcccac
     1741 ccgccattgc ggcgcgagcc caatagtcat gggcttccac gccttcccca tctgtatgtt
     1801 catccccgcc cgtcattcca cccccacacg cgctcaataa aagcgcacct gccaaaataa
     1861 aagcaaaaat ctttttcatt ctcttatcct cgtatcgtat tttttctgaa tcacatgcca
     1921 gaatgcatgt gcatcattcc acgcatatgc tggatggttt catccatgcc aagtaattga
     1981 ccgccaaacc ctttctgaaa cttttctgct tcgactctgg aaccaaacac aagcacacta
     2041 ggtacacaac aaatgctaac ctcgctgcct accagataga ttgcctgccc ggcgctgatc
     2101 atatgcccat acaaataatc tgcggtcaac gattgccaaa cgctttctga acggctttgc
     2161 aacattagcc cacaatgtga acagcacgcc cgaagttcat ctccattttc cagtttcaca
     2221 ataaaaacag ttcgctgaga aaccggttta ctgcacatgg cacaataaga agaatcttca
     2281 gtttgtctac tggtagaagg caacctaacg ccgccatgtt tttttatcac ccgcccagct
     2341 tctgcaagtt tgtttaggtc gcggtgaatg gtcatgttgg aaacgcccaa gacctccaca
     2401 agttcttgaa tggaagcgtt accatgcttt tgcaaatggt ctagaatttg ttgttcgcgg
     2461 tttaatgagt ttggggacat tttgttatat tttgttatct ttaacaaaga atgacgaaat
     2521 ataacaactc aacctgcatt tatcaagatg acatttgtca ctactttgag tgatactttc
     2581 ttccgcgcca ggtaaccccc tgcccggtca atactttcca ggcagaagta agcatcatgg
     2641 cagcaaaaac ccccgcccct agtggagtcg tccacgcata ccaacgcgga atctccatct
     2701 cttttgccac cataaaacgg aaatagatca aatatcccca caccagcaat gcttcaacga
     2761 tcacagccca tgccatccaa ccgccatgat tgaaatacca tgccaacccc aacaacggcc
     2821 acactggcag gaacaatgcg gcgaataatg ccaaggtcgc gccaaacgcg cccaacaaca
     2881 atagcgatgg atggtcacgc aaaccaagat aaatattctt cgtccaacct tcccacatgg
     2941 tttcgagcga cgtgtacatg cgtgtgctag ccaccaaacg cccatctgcc accaccaaac
     3001 gatgaccatt ccactttaca ttttcagaga gggctttgtc ttctacaatc tggtctttta
     3061 ttttttcatg cccgccgatc aaatcataga tgctgcgttt gataaaaatg aattgtccat
     3121 ttgctactgc atcacggcgc gctgggtcat tgaccttgcg cggagagaat cccactgaga
     3181 gcgcggtcat caccaatggc atcactgtca attcccaaaa cgaaccgagg atttgccggg
     3241 tcatcatggt gaacaagtcc gctttggttt cgagcgcttt ggcatacacc gatgaaagag
     3301 cattgggctc gagaaaagta tcggcatcca caaagcaaag ccattcgcct tttgccacgg
     3361 ctgaggcttg atacaaagca tggggtttgc ccgcccagcc ttcgggtaag tctgagccgc
     3421 tgattgggag gagacgagag tcgcgggatg cgatctcttt caatagagtc agagtggaat
     3481 cggtggagcg atcatccaaa acgatgattt caaaatttgg gtaatcttga cgaagaaccg
     3541 cctcaacaca acgccgaatg ttggtctctt cgttgcgagc cgggatacac accgaaacct
     3601 tcggcgcgga cgtgggcgca ggcaccggcg tgacgacgat atccagatga tattggttat
     3661 gaatccagta gatgatgatg agggcgagga ggaaaaggat ggtggaga
//
LOCUS       DAOQNJ010000229         3708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_16565_length_3708_cov_7.220914, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000229 DAOQNJ010000000
VERSION     DAOQNJ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16565_length_3708_cov_7.220914"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="PLG52_07605"
     CDS             <1..1379
                     /locus_tag="PLG52_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNB41350.1"
                     /translation="NKKSLFTLLSLLVLASMVLAACGTPAATEAPAEPAATEAAAATE
                     APAATEAASALPEGDGEVTILGGYGEADAKNFQQVLTEFGDANGIKITFTSLASFDTD
                     IKVKIESGQEPDIALWPQPGGLKDLATDNLVPLNQVFDTSVPLGTLVPGWDKLAVVDG
                     ELYGLPVSANMKSLVFYNPSAFEAAGYSVPTSEAELQALEEQIKADGTGYPWCAGIES
                     GGATGWAFTDWMEQYVLMFNGPEVYADWISGKVDFASPEITKAADYVTARLLAEGQVN
                     GGGIAMATDSFGDTAPLFANGKAEGQCFMMRQGSFIVGFMPEDIQAEVAAGDYTHLDA
                     FPLPTPDGAQAGVIGGGDLFAVFQGHVDQDVAKVAAFIASDQVLKYMVSAGAISPHKT
                     FDPALYPNELNRKIGEAMAAAAVFGFDGSDQMPAEVNAEFWAAGTDYVAGRATWADAA
                     ARIDSKYP"
     gene            1512..2492
                     /locus_tag="PLG52_07610"
     CDS             1512..2492
                     /locus_tag="PLG52_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNB41351.1"
                     /translation="MGDIIFGLYSIVLGIAVSYALYWLLNFLLSLLPQRIFQKVSWLS
                     FLTPAAVLVIVVLIAPLFQTVIWSFKDDKVKEWVGFKNYVDLFTDIRFLGILLNNFLW
                     VLVVPAITVAIGTAAATLSNQVGPTREKIFKSLIFMPMAISFVAASTIWAYFYAYVPP
                     GRPEIGFLNSVVISLGFEAQPWLTMDQGRLNSFLLMIVLIWLQVGYSMVVISAAIKNV
                     PEETLEAAQLDGANAWNIFSKIVLPQVWGTVMSVFVTVLILVMKIFDIVLAMTRGNFT
                     TSVLAFEWYKQFFENSNIGPASAVVTVLVILIGPLMYLQIRTVQQQELLR"
     gene            2489..3364
                     /locus_tag="PLG52_07615"
     CDS             2489..3364
                     /locus_tag="PLG52_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNB41352.1"
                     /translation="MNMEKISHLIRKIKLGSTLGMLVLVTIWSIPTVGLLITSLRPSS
                     VAEISPWWEALANPLGQNWTIDAYQTSLEKGMLGSMINTIAVTVPSTVLPLMIAANAA
                     FAFTFFKFKGKEIIFAVLVGLMVIPLQSSLIPVLRWMVVFQKAAHIKLTGTFVSAWLV
                     HGAFALPLAIYTLRNYMMTLPHELIESAKVDGASYYQIFWKLVLPLSMPAVASFAIFQ
                     FLWVWNDYLIAFIFVGEQKAVMTYRLLRLLGQYGEGWRDVAAGSFVSLIIPLIVFFSL
                     QRFIVRGLLAGSVKG"
     gene            3410..>3708
                     /locus_tag="PLG52_07620"
     CDS             3410..>3708
                     /locus_tag="PLG52_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNB41353.1"
                     /translation="MNQSLWYKNAVFYQISVRAFKDSNGDGHGDLQGLTEKLDYLKDL
                     GVDCIWIMPIYPSPLRDDGYDIADYYSVDKTYGSLDQFKALIEAAHQRGIRIITDL"
BASE COUNT          785 a          884 c          937 g         1102 t
ORIGIN      
        1 tgaataagaa atcccttttt acccttttga gcctgctcgt tttggcttcc atggtactcg
       61 ccgcatgcgg aacccccgct gcgaccgaag cccccgctga acccgccgca accgaagctg
      121 ccgctgctac tgaagcccct gcagccaccg aagccgccag cgccctccct gaaggcgatg
      181 gcgaagtcac cattttgggt ggctacggcg aagcagatgc taagaacttc caacaagttt
      241 tgactgaatt tggtgacgcc aacggcatta agatcacctt cacctccctc gcttcttttg
      301 ataccgatat caaagtcaag atcgaatccg gtcaggaacc cgatattgct ttgtggcctc
      361 agcccggtgg tttgaaagat ctcgccaccg ataacctcgt tcctttgaac caggtcttcg
      421 ataccagcgt tcctttggga actttggttc ctggttggga caagttggct gttgttgatg
      481 gcgaacttta tggtttgccc gtttctgcca acatgaagtc tttggtgttc tacaatcctt
      541 ctgctttcga agctgctggt tactctgttc ctacctctga agctgaactt caggctctcg
      601 aagaacagat caaggctgac ggcaccggtt acccctggtg tgctggtatt gaatctggtg
      661 gcgctaccgg ttgggcattc accgactgga tggaacagta tgtactcatg ttcaatggcc
      721 ctgaagtgta tgctgactgg atctccggca aagttgattt cgcttccccc gaaatcacca
      781 aggctgctga ttatgttacc gcccgccttc tcgctgaagg tcaggtcaac ggtggtggta
      841 tcgccatggc aaccgactcc ttcggtgaca ccgctcctct ctttgccaat ggcaaggctg
      901 aaggtcagtg cttcatgatg cgccaaggct ccttcatcgt tggcttcatg cccgaagata
      961 ttcaggctga agttgcagct ggtgattaca cccacctcga tgccttccca cttcctaccc
     1021 ccgatggtgc tcaagctggc gtgatcggtg gtggtgacct cttcgccgtc ttccaaggtc
     1081 atgtggatca ggatgttgcc aaggttgctg ctttcatcgc tagcgatcag gttttgaagt
     1141 acatggtctc tgctggtgct atctccccgc acaagacctt tgatcctgct ctctatccca
     1201 acgagctgaa ccgcaagatc ggtgaagcca tggctgctgc tgcggtcttc ggtttcgatg
     1261 gctctgatca gatgcctgct gaagtgaacg ccgagttctg ggctgccggt accgactatg
     1321 tcgccggtcg cgctacctgg gctgacgctg ccgctcgcat tgacagcaag tacccgtagt
     1381 cgtacggtaa gttaggtaaa atagtggcgc cgactcttgt tcttgatggg gtcggtgcca
     1441 catctttttt actcgtttcc ccatggtgac taggtagtaa tcgataaatc cgaatgaatg
     1501 gagattgaga tatgggcgac attatttttg ggctttattc catagtcctt ggtattgcag
     1561 tcagctatgc tctgtactgg cttctgaatt tcctgctttc ccttcttccc caacgtattt
     1621 ttcaaaaagt gagttggttg tcttttttga cgccagcagc ggttttggtg atagtggttt
     1681 tgattgcacc gctattccag acggtgatct ggtctttcaa agatgacaaa gtaaaagaat
     1741 gggtggggtt caaaaattat gtagacttgt ttaccgatat acgttttttg gggatattgt
     1801 tgaataactt cctatgggtg ttggttgtgc ctgccattac ggtggcaatt ggtactgccg
     1861 cagccacgct ttccaatcag gtgggaccaa cccgtgagaa aattttcaaa tcattgatct
     1921 ttatgccaat ggcgatcagc tttgtggctg catctaccat ttgggcttat ttttatgcat
     1981 atgttccgcc gggacgcccg gaaatcggct ttttaaattc tgttgttata tccctgggat
     2041 ttgaagcgca accttggttg acgatggatc agggtcgttt aaattccttt ttacttatga
     2101 ttgtgctaat ctggctgcaa gtgggctatt ccatggtggt gatttcagcc gcgattaaaa
     2161 atgtacccga agagaccctg gaagctgccc aattggatgg cgccaatgct tggaatattt
     2221 tttccaagat tgtcctgcct caggtatggg gcaccgtaat gtcggtcttt gtgaccgtgt
     2281 taattcttgt catgaaaata tttgacattg tacttgccat gactcgtgga aatttcacaa
     2341 ccagtgtatt ggcgtttgaa tggtacaagc aattctttga gaattccaac atcggacccg
     2401 cctctgctgt ggtgacagtg ttggtcatat taattggccc gcttatgtat ttgcaaatcc
     2461 gtacagtaca acaacaggag ttgttgcgat gaacatggaa aaaatttctc acctgatacg
     2521 aaaaataaaa ttaggtagca cattggggat gctggttctg gtgaccatct ggtccattcc
     2581 cactgtgggt ttgttgatca catctctgcg cccgagttct gttgctgaaa tatctccttg
     2641 gtgggaagct ctggcaaacc cgttggggca gaactggaca attgatgcgt atcaaacttc
     2701 ccttgaaaag gggatgttgg ggtcaatgat caacacgatc gcagtgactg taccctccac
     2761 ggttttgccg ttgatgattg ctgccaatgc cgcatttgca tttaccttct tcaagtttaa
     2821 aggtaaggaa attatctttg cggttttggt cggtttaatg gttattccat tgcagtcttc
     2881 gcttatccct gtgctgagat ggatggttgt gtttcaaaaa gctgctcata ttaaacttac
     2941 gggcacattt gttagcgcct ggcttgttca cggggcattt gcgttgccat tagcgatcta
     3001 taccctccgt aactatatga tgaccctgcc tcatgagcta atcgaatctg ctaaggtgga
     3061 tggtgccagc tattaccaga ttttttggaa gttggtgttg ccgttgtcca tgcctgctgt
     3121 ggcttccttt gccattttcc aattcttgtg ggtttggaac gattatctaa tcgccttcat
     3181 ttttgtgggt gaacaaaagg cagtgatgac ctatcgtctg ttgagattgt tgggtcagta
     3241 cggcgaaggc tggcgcgatg tggcggcggg ttccttcgtc tctctcatta ttccgttgat
     3301 cgtgttcttc agcttgcaac gctttattgt acgcgggttg ctggcgggct cagtcaaagg
     3361 ataactttcc aaaaggacaa gtggactggg caaccagtcc actttttata tgaatcaatc
     3421 tctttggtac aagaacgcag ttttttatca gatctccgtc cgcgcattta aggatagcaa
     3481 tggtgatggt cacggtgact tgcaagggtt gaccgaaaag ctcgactatc tcaaagacct
     3541 cggcgtggat tgcatttgga tcatgccgat ctacccgtcg ccgctgcggg atgatggcta
     3601 tgacattgcc gactattaca gcgtggataa aacctacggg tcgctggacc agttcaaggc
     3661 gttgatcgag gcggcgcatc agcgcggcat ccgcatcatc acggacct
//
LOCUS       DAOQNJ010000230         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_16614_length_3701_cov_7.838179, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000230 DAOQNJ010000000
VERSION     DAOQNJ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16614_length_3701_cov_7.838179"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLG52_07625"
     CDS             complement(<1..232)
                     /locus_tag="PLG52_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNB41354.1"
                     /translation="MSIQPARTRFAPSPTGHLHLGGARTALYAYLLAKKTGGQFILRI
                     EDTDLKRTVPGAEQEIMDGLRWLGLNYDEGPDI"
     gene            complement(252..1379)
                     /locus_tag="PLG52_07630"
     CDS             complement(252..1379)
                     /locus_tag="PLG52_07630"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNB41355.1"
                     /translation="MTRGLSWRNFDFLLLGAVILASAFGVVMIRSAIAGNEVLAISSS
                     RQIYFAMLGVVLIFFLASIDYRYWLALDRPIHLVILGFLVTLTGFGQTAFGAQRWFQV
                     GVLFLQPTEFAKIVALIVLAKYFDSVRDQPRDLRWIFGAILRAMWIIGLILAQPNLSN
                     VMVLSVILAVMLWVNGIQVKHVITAVLLGALAVISVIALSVLGVRIPFLQAYQQERIV
                     NFVVPDPNDTYGNRYNVQQALIAVGAGGFSGEGYGYGTQTQLRFLKVRHTDFIFAATS
                     EEFGFVGSVSIILLLGVVVWRCIRAAQKARDVAGAMLAIGVATLIFFQGAVNIGMNLN
                     LLPVSGLPLPFVSYGGSGLTALMIAIGLVESVVMRQKDLEF"
     gene            complement(1443..1694)
                     /gene="minE"
                     /locus_tag="PLG52_07635"
     CDS             complement(1443..1694)
                     /gene="minE"
                     /locus_tag="PLG52_07635"
                     /inference="COORDINATES: protein motif:HMM:NF001422.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNB41356.1"
                     /translation="MDFFTRKRSATSAKERLQLVLVHDRTDLTPGQLESLKDELLKAI
                     SKYIEIDPEAVRIELEKDGREQRLVADIPLKGATRHRAG"
     gene            complement(1696..2493)
                     /gene="minD"
                     /locus_tag="PLG52_07640"
     CDS             complement(1696..2493)
                     /gene="minD"
                     /locus_tag="PLG52_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNB41357.1"
                     /translation="MTAQVITVTSGKGGVGKTTAVANLATALAMDGKRVVCIDGDIGL
                     RNLDVVMGLENRIVYDIVDVIEGRCKLKQAMIRDKHYQDMYLIPAAQTRDKNAVSPSD
                     MIRICNDLRPDTDFIVIDSPAGIERGFRNAIAAADSVLVVTNPEVSAVRDADRVVGIL
                     EAEEKGNPALIINRLNPALVKNNDMLSAEDVLDLLGIRLIGIVPEDENVIIGSNRGTP
                     IAADGKSRAGQAFRNIAKRLQGVDVPFMDLTPQSGLWNTIQRLAGRK"
     gene            complement(2495..3205)
                     /gene="minC"
                     /locus_tag="PLG52_07645"
     CDS             complement(2495..3205)
                     /gene="minC"
                     /locus_tag="PLG52_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNB41358.1"
                     /translation="MNIPMESTTSLVQIKGIRDGLLATFAEAAWEEQRQALMEQINER
                     VSFFQGARLAMDVGTQVLKVNDLVDLRDWLSERNITLWAIVSESPTTETTAQLLGLAT
                     RISKPRPEEVRHVHEAVSSDTALFVSKTVRSGTRIEYPGTIVVFGDVNPGAEIVAEGN
                     VIVWGRVRGVIHAGVKGDRSAFICALDLSANQLRIANESSAMLKPQKDPKPEIASINS
                     EGKLQAELWQTGETLIRK"
     gene            complement(3247..>3701)
                     /locus_tag="PLG52_07650"
     CDS             complement(3247..>3701)
                     /locus_tag="PLG52_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNB41359.1"
                     /translation="KWDITVDPRIQEYSCEGPYCALTDDFKVVKPETVEAVRAGTRRA
                     VTDTSFGTLYRLFKDFPIAVAGKTGTAEYCDDVARKADRCNFGAWPTHSWTIAYAPYD
                     DPEIAIIAFAYNGGEGASVAGPMVARMIKAYFEIKALDIAQGVNAPAP"
BASE COUNT         1049 a          955 c          802 g          895 t
ORIGIN      
        1 caatatctgg accttcatcg tagttcagtc caagccaacg cagaccatcc atgatctctt
       61 gttcagcacc ggggacagta cgcttcagat ctgtatcttc gatacgcaaa atgaactgtc
      121 cgccggtctt tttggctaac aggtaggcat acaaagctgt acgtgcacca ccgaggtgca
      181 aatgcccggt tggcgagggt gcaaagcgag tgcgagctgg ctgaatggac aaaatttcac
      241 tcctacttaa tttagaattc caagtctttc tgacgcatga cgacggattc taccaaacca
      301 atggcgatca tcagcgccgt cagaccactg ccaccgtaac ttacaaatgg taacggcaaa
      361 ccagaaacag gcaataagtt taggttcata cctatattca ccgccccctg aaaaaagatc
      421 aatgttgcta cgccaatagc aagcatggct cctgcaacat cacgcgcttt ttgtgccgca
      481 cgaatacagc gccaaacaac cacacccaac aaaaggatga tcgatacaga ccctacaaag
      541 ccaaattctt ctgatgttgc cgcaaagata aagtctgtat ggcgcacttt aagaaagcgt
      601 aattgtgttt gtgtgccgta cccataccct tcgccagaaa aaccgccagc ccccacagcg
      661 atcagagctt gttgcacgtt ataacggtta ccataggtat catttgggtc gggaacgacg
      721 aagttaacga tacgctcttg ttgatatgct tgtaaaaacg gaatccgtac accaagcact
      781 gaaagggcga tcacactaat caccgctaaa gcacccaaca gcacagctgt aataacatgc
      841 tttacttgaa tcccatttac ccaaagcatg actgctaaaa tgaccgaaag aaccatcaca
      901 ttacttaagt ttggctgcgc tagaatcaaa ccgatgatcc acatggcgcg cagaatagca
      961 ccaaaaatcc aacgtaaatc acgtggttgg tcacgaactg aatcaaagta cttggcaagc
     1021 acaatcaatg caacaatctt ggcaaactcg gtaggctgca gaaagagcac acctacttga
     1081 aaccagcgtt gggcaccgaa tgcggtttga ccaaaaccag tcaatgtgac gagaaagcct
     1141 agaataacaa ggtgaattgg acggtcaagc gcgagccaat aacgataatc aatagaagcg
     1201 aggaagaaaa tcaacaccac gccaagcatg gcaaagtaaa tctggcgtga cgacgaaatt
     1261 gccagcactt catttccggc aatcgccgag cggatcatca caacgccaaa agcagacgct
     1321 aaaataactg cgcctaacaa aagaaaatcg aaatttctcc aggaaagtcc acgtgtcata
     1381 ataaaaaaac atggatgaac ccgtatcgct acaggttgat ccatgtccta ttcaaaaaca
     1441 tatcaacctg cgcgatgacg cgttgcgccc tttaatggaa tatctgccac caaacgttgc
     1501 tcgcgtccat ctttttccaa ttcaattcga acagcttcag ggtcaatctc aatatacttg
     1561 gagattgcct tcaagagttc atccttcaaa gattctaatt gcccaggggt caggtccgta
     1621 cggtcgtgaa ccaacaccaa ctgtaatcgt tcttttgcgc tcgtagcgct ccgcttgcgt
     1681 gtgaagaagt ccatatcact tccttccagc caaacgttga atggtattcc acaagccact
     1741 ctgcggagta aggtccataa atgggacatc aactccttgc aagcgtttgg caatattacg
     1801 gaaagcctgc cccgcacggc tctttccatc ggctgcaatc ggagtcccgc ggttcgaacc
     1861 gatgattaca ttttcatctt ctggcacaat accaatcaaa cgaatgccta aaagatccaa
     1921 cacatcttcc gcagaaagca tgtcgttgtt tttcacaagg gcaggattca gacgattgat
     1981 gataagcgcc ggattgcctt tttcttccgc ttcaagaatg cctaccactc ggtctgcatc
     2041 gcgcaccgca ctgacttcgg ggttggtcac tacgaggacg ctgtctgctg cggcgatggc
     2101 gtttcgaaat ccgcgctcga tacctgcagg ggagtcaatg acgatgaaat cagtatccgg
     2161 tctgaggtca ttgcagatgc ggatcatgtc agatggcgag acagcatttt tgtcacgtgt
     2221 ttgagccgcc ggaatcaggt acatatcctg ataatgctta tcgcgaatca ttgcctgttt
     2281 gagtttgcaa cgtccttcga tgacatccac aatatcgtag acgatacgat tctcaagacc
     2341 catcacaaca tcaaggttac gcaacccaat atcaccgtca atacaaacga cccgtttgcc
     2401 atccatggca agagccgtag caagattggc aactgcagtg gtctttccca cgccgccttt
     2461 tccagaagtt acagtgatca cttgagctgt catattattt cctaattaaa gtttcgccgg
     2521 tctgccataa ttctgcttgc aatttacctt cgctgttaat gctggcaatc tcgggctttg
     2581 ggtctttctg tggtttcaac attgcggatg actcatttgc aatccgtaat tggtttgcag
     2641 ataggtctag ggcacaaata aaagcggagc gatctccttt tacgccagca tggatcactc
     2701 cacgcacacg cccccaaacg atcacatttc cttctgccac gatctctgcg ccggggttta
     2761 catcgccaaa cacaacaatc gtacccggat attcgatgcg tgtaccagag cgaactgttt
     2821 ttgaaacgaa aagtgcagtg tcgcttgata ctgcctcatg gacatgacgg acttcttccg
     2881 gacgcggttt ggaaatacga gttgccaaac ctagtaactg tgctgttgtt tctgtagtag
     2941 gcgattcgct gacgattgcc cataaggtga tattccgttc agaaagccaa tctcgcaggt
     3001 caaccaagtc attgaccttc aaaacctgtg tacccacatc catggcaagg cgtgctccct
     3061 gaaagaaaga gacgcgctca ttgatctgct ccataagagc ttggcgctgt tcttcccaag
     3121 cagcctcagc aaaagttgca agcagaccgt ctcgaatgcc tttgatttgg acaagagatg
     3181 tagtagattc cataggaata ttcattcagg taatggttaa taattatacc tgatgaaatt
     3241 agcgtgttat ggcgccggcg catttacacc ttgagcaatg tctagagctt tgatctcaaa
     3301 atacgcctta atcatacgtg ccaccatagg acctgccaca ctagcacctt caccgccgtt
     3361 ataagcaaaa gcaataatag cgatttcagg gtcatcataa ggagcgtacg caatcgtcca
     3421 tgagtgagtg ggccaggcgc caaagttaca acggtcggct ttgcgggcaa cgtcatcaca
     3481 atattcagct gtcccggttt tccctgctac agcaataggg aaatctttaa atagccgata
     3541 tagggtacca aatgaagtat ctgtcactgc acgccgggtt cctgcgcgaa cagcctctac
     3601 tgtttcgggt ttaaccacct tgaaatcatc tgttaatgca caataaggac cttcacagga
     3661 atattcttgg atgcgcgggt caacggtgat gtcccacttc a
//
LOCUS       DAOQNJ010000231         3686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_16719_length_3686_cov_9.580281, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000231 DAOQNJ010000000
VERSION     DAOQNJ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16719_length_3686_cov_9.580281"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..434
                     /locus_tag="PLG52_07655"
     CDS             174..434
                     /locus_tag="PLG52_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNB41360.1"
                     /translation="MTNHTHQNCQALLGSLSEYIDGELPPDLCKEIEKHLEGCDNCRI
                     VLNTTRRTIDLVQIPVEENVPDDVRERLFKRLNLDDYLKQQS"
     gene            456..1637
                     /locus_tag="PLG52_07660"
     CDS             456..1637
                     /locus_tag="PLG52_07660"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNB41361.1"
                     /translation="MKTIQNFAFLASLLLISACQTLPVETAAISPTTLAETVIPSNTP
                     APSPTPNFTPTVPAAVIELSGTTLPTGFSILKFAEIYRPTALTVDLNGNILVASADSN
                     IYIVSDADKDGHADTQSIFASGYYIPLGLTVHPATGDVYVSYQGAIAVLTDSDKNGEA
                     DSSRILVEGLPSTGRHQNNNLEFGPDGWLYMGMGSTCDACAEADARNATIMRFNLETG
                     EKEIFASGLRNPFDIAFHPLTGELFSTDNGRDDLGLDMPNEELNLILQNGSYGFPNCW
                     NEQNTNGCENTIPAVAFFEPHSSADGLDFYKGSQFPIEYQNNAFVGIFGSWLNPTLET
                     GIKRVILTPKDGIYTSEVSWFAKFPKGVMPLPLQFGPDGALYVGDYINDVVYRISYGV
                     P"
     gene            complement(1672..1742)
                     /locus_tag="PLG52_07665"
     tRNA            complement(1672..1742)
                     /locus_tag="PLG52_07665"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1708..1710),aa:Gln,seq:ctg)
     gene            complement(1792..2130)
                     /locus_tag="PLG52_07670"
     CDS             complement(1792..2130)
                     /locus_tag="PLG52_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41362.1"
                     /translation="MSQSDQNKTNFAGTYFDKDAVIRIAKLLKLASWIVAGVYFFQFV
                     VNVGIFTLQIFRKLLYFGGPTDFMQQLLWQVQPSLAGLIFFASLQAIALALLILLDIE
                     DNTRRAARGK"
     gene            2287..>3686
                     /locus_tag="PLG52_07675"
     CDS             2287..>3686
                     /locus_tag="PLG52_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNB41363.1"
                     /translation="MSPEENSKPSDFIREAVAEDLKSGRFNYVRTRLPPEPNGYLHIG
                     HVKAFMIDYLVAKENGGELVLRFDDTNPAKEETEFVQAIEEDARWLGIEWVKVTYASD
                     YFDELYDWAKKLVLMGKAYVDDQTPEEVSKNRGTLTQPGVNSPYRDRDVEENLDLLER
                     MKNGEFPDGTRILRAKIDMAHPNINMRDPAMYRIIHTPPHHRTGDKWKIYPMYDWSHG
                     QNDSMEGITHSLCSLEYENHRPLYEWFLEQLGVFKPRQIEFARLNMNYTVMSKRKLRR
                     LVEEKLVSGWDDPRMPTLRGMRRRGYTAEAVLDFIRRVGVAKNYSVSDVALLEECVRD
                     DLNKHALRRMAVIRPLKVVIDNYPDDLVEEMSAINNPEDAEAGTRKVPFSKVLYIEQD
                     DFREVPPAKYYRLFPGNEVRLRYAYFIKCTHVVKNDAGEVIEVHATYDPSTKGGDSPD
                     GRKVKSTIHWVSAKHA"
BASE COUNT         1050 a          839 c          841 g          956 t
ORIGIN      
        1 cttcgaatgg tattcattct ccgtgatgta gaagaccttt cgatcaaaga aacggctgag
       61 attttaaatt tgactgaagt gaatgtgaaa actcgtttat tgcgtgcccg tatggcttta
      121 cgtgaacaac tatcctctta ttatgcagag cggacccgca gaaaggaaac ccaatgacca
      181 accataccca tcagaattgc caagcgctac taggctcttt atctgaatat attgatggtg
      241 aactgccgcc cgatttgtgc aaagagatcg agaagcacct tgaaggttgt gataactgcc
      301 gcatcgtact caataccact cgccgtacta ttgacctagt acaaattcct gtggaagaaa
      361 atgttccaga tgatgtaagg gaacgcttat ttaaacgact taacctggat gattacttaa
      421 agcaacaaag ttaattgcca ctataatcag atgtcatgaa aacaattcaa aattttgctt
      481 tcctggcatc tttgctttta atctcagcat gccaaacttt acctgttgaa actgcggcta
      541 tctcccccac aactctggct gaaacagtga ttccatcaaa tacccctgct ccatcaccta
      601 cacccaattt cacaccgacc gtcccagccg ctgtgatcga actttcaggg acaactttac
      661 ctactgggtt ttcgattctc aagttcgccg aaatttaccg tcccactgcg ttgacagtag
      721 acctcaatgg aaacatcctc gtcgcttcgg cagatagcaa tatttatatc gtatcagatg
      781 cagataaaga tggtcatgcc gatacgcaat ctatttttgc atccggatac tatattccgc
      841 tcgggttgac cgtgcacccc gcaacagggg atgtatatgt ctcatatcaa ggcgcaattg
      901 cagtactaac agattcagac aaaaatggag aggcggattc cagccgcatt ttggtggaag
      961 gtttaccgtc tacagggcgg catcaaaata acaatcttga atttggtcca gatggctggt
     1021 tatacatggg gatgggctcc acttgtgatg catgtgccga agccgatgcg agaaatgcaa
     1081 caatcatgcg tttcaaccta gaaacaggcg aaaaagaaat ctttgcttct gggctgcgca
     1141 atccgtttga tatcgctttt cacccgctta caggcgaatt attttctacg gataatgggc
     1201 gtgatgacct tggtttagat atgccaaacg aagaactaaa ccttattctt caaaatgggt
     1261 cctatggctt tccaaattgt tggaatgaac aaaacaccaa tggttgtgaa aacacaattc
     1321 ctgctgtggc attttttgag ccgcattctt cagcagatgg tttggatttt tacaaaggaa
     1381 gtcaatttcc gatcgaatat cagaataatg cctttgtagg aattttcggc tcttggctaa
     1441 acccgacact tgaaaccggt atcaagcgag tgatccttac gccgaaagat ggtatatata
     1501 ccagtgaagt ctcttggttt gccaaattcc ctaaaggagt catgccctta ccactccaat
     1561 ttggtcctga tggtgcattg tacgttgggg attacatcaa tgacgtagtg tatcgcatca
     1621 gttatggagt gccgtaaata aaaaaactcc ctaaaccaag ggagttttgt gctggggatc
     1681 aaggattcga acctcaaatt tcgcgtccag agcgcgacgt gatgccattt caccaatccc
     1741 caattttcca gcgggcggta ttttatcacg caccctcaga gagggcaagc cctattttcc
     1801 gcgtgcggca cgacgggtgt tatcttcaat gtcgagaaga atgagaagcg ccagtgcaat
     1861 cgcttgtaaa gaggcaaaaa atatcaaccc tgccaaactc ggttgaactt gccagagtag
     1921 ttgttgcata aaatcggtag gtccgccaaa gtaaagaagt ttgcggaata tttgcaaggt
     1981 gaaaatacct acattcacta caaattgaaa aaaatacact cctgctacaa tccatgaagc
     2041 gagtttaagg agctttgcaa tgcggatgac agcatctttg tcaaagtagg ttccggcaaa
     2101 attggtctta ttttggtcag attgagacat acattcctcc agagaatagt tattatactt
     2161 tgttataatt agcacgaata cgacgctccc agcggggaca aagttcctga aaggctggca
     2221 ggtgtcggct agaaaagagg cggaaattcc ttccgcataa atccatcatg aaggttaagt
     2281 caaagaatga gtcctgaaga aaatagcaaa ccgtctgatt tcatccgcga agctgtggca
     2341 gaagacttaa agagcgggcg cttcaactac gtccgtacac gcctgcctcc tgaacccaat
     2401 gggtacttac acattgggca tgtcaaggcg ttcatgatcg attatttggt tgccaaggaa
     2461 aacggcggcg agttggtttt gcgttttgac gataccaacc ctgccaaaga agagactgaa
     2521 tttgtgcaag ccatcgaaga agatgcacgt tggcttggga ttgaatgggt aaaagtaaca
     2581 tacgcatcag attattttga tgagctttac gactgggcaa agaaactggt tttgatgggc
     2641 aaagcttatg tagatgacca gaccccagag gaagtaagca agaatcgcgg cacgctcacg
     2701 caacccggcg taaactctcc ctatcgcgac cgcgatgtag aagaaaactt agacttgctc
     2761 gaacgcatga agaacggcga attccctgac gggactcgta ttttacgcgc caagattgat
     2821 atggcgcatc cgaatatcaa catgcgtgac ccggcaatgt atcgcatcat ccacaccccc
     2881 ccgcatcacc gtaccggtga caagtggaag atttacccaa tgtatgattg gtctcatggg
     2941 cagaatgatt cgatggaagg tatcactcat tcgttatgtt cgcttgagta tgagaatcat
     3001 cgtccattat atgaatggtt cttagagcag cttggcgttt tcaaaccacg tcagatcgag
     3061 tttgcgcgtt tgaacatgaa ctatacagtg atgagcaagc gcaaacttcg ccgccttgtc
     3121 gaagaaaaac tggtcagcgg ttgggatgac ccccgtatgc caacattgcg cggtatgcgc
     3181 aggcgcggct acaccgctga agcggttttg gatttcattc gccgtgtggg tgtggcaaaa
     3241 aattatagcg ttagtgatgt ggcactactc gaagaatgtg tacgtgatga tctcaacaaa
     3301 catgctttgc gtcgcatggc ggttatccgc ccgctgaaag tagtcatcga taattaccct
     3361 gacgacctcg ttgaagagat gagcgccatc aacaaccctg aagacgctga agccggcacc
     3421 cgcaaagtgc ctttctctaa agtcctttac attgaacagg atgatttccg tgaagttccg
     3481 cccgcgaaat attaccgtct cttccccggt aatgaagtcc gcttgcgata tgcatatttc
     3541 atcaaatgca cccatgtagt gaaaaatgat gctggtgaag tgatcgaagt ccatgccact
     3601 tatgacccat ccacaaaggg tggcgattca cctgatgggc gaaaagtgaa atctacgatc
     3661 cattgggttt ctgctaaaca tgcaaa
//
LOCUS       DAOQNJ010000232         3647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17024_length_3647_cov_11.289532, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000232 DAOQNJ010000000
VERSION     DAOQNJ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17024_length_3647_cov_11.289532"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLG52_07680"
     CDS             complement(<1..394)
                     /locus_tag="PLG52_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB41364.1"
                     /translation="MSKKKSTRKTSSMAQGGLSSFLRKPTGQVTVIAVLALVIYLIAS
                     SVRGGSSTGLASTVSADEAYQMYQNGTFVLDVRTQAEWDDYHVPNTTLIPLDELPNRL
                     SELPKDKEIVVICHSGNRSQQGRDILLNA"
     gene            complement(527..934)
                     /locus_tag="PLG52_07685"
     CDS             complement(527..934)
                     /locus_tag="PLG52_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB41365.1"
                     /translation="MQLSFSKQDNVTVLHVMGDVDSSNYTEVIIKAQQLYDDGTRNLL
                     LDLANVPYVSSAGLMAFHTIARVFAGHSIQGGESGRPTFRSINPKDDASAREHVKLLS
                     PQPAVEQVLDVIGLKQFFEIYTDLKVAVQSFTS"
     gene            complement(951..2228)
                     /locus_tag="PLG52_07690"
     CDS             complement(951..2228)
                     /locus_tag="PLG52_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNB41366.1"
                     /translation="MKRNLGNIVFTILVLLTIVVWVVFPPHSEGVENYLRYHIGMTMG
                     SVVIALMSFSLFISTRPRWAEPYFGGLDKMYMTHRYTSTAAFLLMFVHLLIVPLHLVN
                     LALGNYLAIVAFLGFIAIVLPTLAPRIPFLNKLTSGTYEGWKRLHGFIGIFFILGYLH
                     SITVNAPTTKIAINWNQIFVFLGIGSYIYTEVLSRFLGKHVPYTVKAVNHPNNSTTEV
                     VMQAKGKPIQHAHAGQFLFVRFKGDKILDEAHPFTISSAPHEGELRVTVKAVGDFTRY
                     LFQNLKPGMDAIVEGAYGMFNYKTGSDKQIWVAGGIGITPFLSFLRDLKTDLAHDVHF
                     YYTVRHPEEAVFADEIETIAKKHPRLKAHIRFSSQHGSLTVDEIVKNAGGDASKHHVY
                     MCGPLPMVQAFASKFVEAGVSKDNVHFEEFNFR"
     gene            complement(2296..2709)
                     /locus_tag="PLG52_07695"
     CDS             complement(2296..2709)
                     /locus_tag="PLG52_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB41367.1"
                     /translation="MEINISQEKGNVPVTVIQVTGQLDGQTYQKLIAAAQEVIKEGST
                     NILLDLSELTFISSAGLVSLHVIALLLRGEAMPDLDHGWAALKSVNKSRESGVQQHVK
                     LLNPREEVINVLDMVGFSSFFEVFKDKQKALESFS"
     gene            complement(2745..>3647)
                     /locus_tag="PLG52_07700"
     CDS             complement(2745..>3647)
                     /locus_tag="PLG52_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNB41368.1"
                     /translation="YAFLVGAARFVDGKFVIKQFFLRDPAEEPAMLEALINFLAPCEG
                     LVTFNGKAFDAPLLVTRYSLHRIPVPFKNYAHIDLLPLARRLWRDRLPSRALKYIEEH
                     VMGFTRSSEEVPGYEIPWMYFDYLRTGDARPLGGVFYHNAMDVVAMAALLAHVSEILA
                     DPYNGRVEHGLDFIALGKLFEDLGHWDEAARLFERGLEIGLDESDFGVAVKRISILQK
                     KRGDMDRAVELWKAAAKKGHIYAHIELAKYYEHKMRDAKSAVKWAKSARAEAEKADLP
                     PYIRNHWFHEIDHRLERLTRKAGL"
BASE COUNT         1019 a          915 c          802 g          911 t
ORIGIN      
        1 ctgcattcag caaaatgtcg cgtccttgct ggctgcgatt gccagagtga cagatcacaa
       61 cgatttcctt atccttcggt aattcactga ggcggttcgg taactcatcc aatggaataa
      121 gcgtagtgtt gggaacatga taatcatccc actctgcttg tgtgcgcaca tccagtacga
      181 aagtcccatt ctgatacatc tgatacgctt catctgcgct gacagtgctt gccagcccgg
      241 tggaactgcc tccacgcaca ctgctggcga tcagatagat gaccaatgcc aaaacggcaa
      301 tgacagtcac ttgacccgtc ggtttacgca agaaactgct taatccacct tgcgccatag
      361 aactcgtttt acgagtagat tttttcttag acattttgcc tcacatcaaa agatttgtat
      421 tcttttgatg ccaaggtcgg tggaaagtta cacttccata taaaaagaaa cggtgacttg
      481 tcagaaaacc tggcaagtca ccgtttgaat ttagaaaaga aggagattaa ctggtgaatg
      541 actgaactgc aactttgagg tctgtataga tctcaaagaa ttgcttcaag ccgatcacat
      601 ccagcacttg ctcaacagca ggttgcgggc ttaataactt gacatgttct cgagctgaag
      661 catcatcctt cgggttgatc gaacggaagg taggtctgcc actttccccg ccttgaatgg
      721 aatgtccagc aaagacacgc gcaatggtgt ggaaagccat cagcccggca ctgctcacat
      781 acggcacatt ggcaaggtca agcaagaggt tgcgagtgcc gtcatcataa agctgttgcg
      841 ctttgatgat cacctccgtg taattagacg agtctacatc ccccatgaca tgcaagaccg
      901 ttacattatc ttgtttcgaa aaagagagtt gcatgggttt actcctacga ctatctgaaa
      961 ttgaattcct caaagtggac gttatctttg gacacgcccg cttctacaaa ttttgacgcg
     1021 aacgcctgca ccatgggcaa aggaccgcac atatagacgt gatgtttgga ggcatctcca
     1081 cctgcatttt tgacgatctc atccacagtc aatgaaccat gctgagaaga gaagcggatg
     1141 tgtgccttca aacgcggatg tttctttgca atggtttcga tctcatctgc aaagactgct
     1201 tcttcagggt gtcttaccgt gtagtaaaaa tgcacatcgt gagcgagatc cgttttcagg
     1261 tcccgtaaga acgagaggaa aggggtaatg ccaatgccgc cagcgaccca aatctgtttg
     1321 tcactgcctg ttttgtaatt gaacatccca tatgcgcctt ctacaatcgc atccatgccg
     1381 ggtttgagat tctgaaacag gtaacgggtg aaatcaccga cagctttaac cgtcactcgc
     1441 aattcgcctt catgcggcgc actcgaaatg gtgaagggat gggcttcatc cagaatttta
     1501 tcgcctttga agcgcacaaa cagaaactga cccgcatgag catgttggat gggcttgccc
     1561 ttggcttgca tcaccacctc agtggtggag ttattgggat gatttaccgc tttcaccgta
     1621 taaggcacat gcttgcctag aaagcggctc aacacttctg tgtaaatgta ggaaccaata
     1681 ccaaggaaaa caaagatctg attccagttt atggcaatct ttgttgttgg ggcattcacc
     1741 gtgatcgagt gtagataacc caaaatgaag aaaataccaa taaagccatg caaacgcttc
     1801 cagccttcat aggttccgct cgtgagttta ttcaagaacg ggatgcgtgg cgcgagcgta
     1861 ggtaaaacga tggcgataaa accgagaaat gcaacgatcg ctagataatt tccaagcgca
     1921 aggtttacca gatgcaatgg cacgatcaac aagtgaacaa acattagcaa gaatgcggca
     1981 gtcgatgtat agcgatgggt catgtacatc ttatccaagc caccaaagta tggctctgcc
     2041 cagcgcggac gagtggaaat gaacagcgag aacgacatca aggcgatcac aacagacccc
     2101 atcgtcatcc cgatgtgata gcgtagataa ttttcaaccc cttcactgtg cggcggaaag
     2161 acaacccaca cgacgatggt aagtaagacc aaaatagtaa agacaatatt cccaagatta
     2221 cgtttcataa aaattcacct ttaatataaa tgacaacgat gcaggcatgc ctgcatcgtt
     2281 gtgggtcgtt tcttcttacg agaaagattc gagagccttt tgtttatctt tgaaaacctc
     2341 aaagaaagaa gagaagccaa ccatatcgag cacgttgatc acttcttcgc gcgggttcag
     2401 caatttcacg tgttgttgaa ctccactctc acgtgatttg ttgaccgact tcaacgccgc
     2461 ccaaccatgg tcaaggtctg gcatggcttc gccgcgcaac agcagggcaa tcacatgcag
     2521 cgacaccaac cctgcgctgg aaataaaagt tagctcactt agatccaata aaatattcgt
     2581 tgagccttct ttgataactt cctgagcagc agcgatcaat ttctgatacg tctgcccgtc
     2641 taattgaccc gtcacctgaa tcacagtaac cggcacattc cccttttctt gtgaaatgtt
     2701 aatttccatg tttcctctcc aaaggttaat ccgtgttcga gcaattataa acccgcttta
     2761 cgcgtcaacc tttccaaccg atggtcaatt tcatggaacc aatgattacg aatataagga
     2821 ggcaaatccg ctttctcggc ttctgcccgt gcagacttcg cccacttcac cgcagacttt
     2881 gcatcgcgca tcttatgctc ataatatttt gccaattcaa tatgagcata aatatgacct
     2941 ttcttcgccg ccgctttcca caactcgaca gcccggtcca tatctccgcg cttcttttgc
     3001 aggatagaga tccgcttcac agctacgccg aaatctgatt catccaaccc gatctcgagt
     3061 ccacgctcga aaagacgtgc cgcctcatcc caatgtccaa ggtcctcaaa tagttttccc
     3121 agtgcaatga aatcaagccc atgctccaca cgtccattat aggggtctgc caaaatctcg
     3181 ctgacgtgtg ccagcaacgc cgccatcgct accacatcca tggcattgtg atagaacaca
     3241 cctccaagtg gacgggcatc gcctgtgcgt aagtaatcga aatacatcca cggaatttca
     3301 taaccgggta cttcttcaga agaacgcgta aaccccatta catgttcttc gatatatttc
     3361 aatgcacgcg aagggagtcg gtctctccag agtcttcgtg ctaaaggcag taaatcaata
     3421 tgggcataat tcttaaaagg gacagggatt ctgtgcaacg agtatcgagt aacgagaagt
     3481 ggtgcgtcga aagctttgcc attgaaagta accaaccctt cacaaggcgc tagaaaattg
     3541 ataagcgctt caagcatggc aggctcttca gccggatctc gcaagaagaa ttgcttgatg
     3601 acgaacttgc catctacaaa acgggcggca cccacgagga aagcata
//
LOCUS       DAOQNJ010000233         3644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17046_length_3644_cov_9.190582, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000233 DAOQNJ010000000
VERSION     DAOQNJ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17046_length_3644_cov_9.190582"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="PLG52_07705"
     CDS             <1..1266
                     /locus_tag="PLG52_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41369.1"
                     /translation="ALALLSGPSVWLGLLGIGITYGIAKLLARRTEESSEEENTPTEK
                     RKRDRSSSAFGKEFFKSSNLITFALAFIFAGSLFFIVPGGIGAAFSSIPAFFKTWAAT
                     GPVTQGMLIISLFVYQPFALLLAGMALLRGWMGGLRRIVFLSIWFFVALLLVIFLSGR
                     QMYDLAWALIPLNALAALELVRHLSIFPEERREVAGVVLLTTFIWVFIWLGTAGFSWI
                     LVDTPDYRLRAGMLVGSIFLLIISLVLVAAGWSIRIARLGGVWGMAICLGLLSVGGLF
                     GATGLRGLSAPELWWLPNVPTQAQLVRDTVHQVSDIGRGSIYSATVAIIGVNSPALEW
                     AVRENPVQVVDAVDPNNTPDFVITSYELDPGLVSAYRGQDFTWRQAPIWNGADTFAWL
                     KWISLRQMPQSQEAIILWARTDLFLGGTQ"
     gene            1333..2013
                     /gene="cmk"
                     /locus_tag="PLG52_07710"
     CDS             1333..2013
                     /gene="cmk"
                     /locus_tag="PLG52_07710"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNB41370.1"
                     /translation="MTKTPPSIIALDGPAASGKSTVGRRLADALGYLFFDTGVMYRAV
                     TWIALNHDMDLKNEAAITQMAESAELDIRPPSQNDGRACDIIVGDKDITWDVRSGDVE
                     ASVSLVAAYAGVRTALSGQQRRIGLRGKVVMVGRDIGTVVLPEADLKVYMDASAEERA
                     RRRFKDITSRGEQANYDELLKKMIERDRLDSTRAVAPLRPADDALVLDTDKLSEEEVF
                     AKILEMCK"
     gene            2017..2727
                     /locus_tag="PLG52_07715"
     CDS             2017..2727
                     /locus_tag="PLG52_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB41371.1"
                     /translation="MSDIVQPPKPVTEIWKPELVRLPELTSLRLAFRRFAYGLVKLVV
                     WSCLRVTREGLENIPSSGPLLIVINHLGHADVPALISSLPYTPDALGEIELYDVPVLG
                     RLMDWYGVIWLHRGRPDKRALRAALDGLAEGRVIVIAPEGRYSLTGALEEGTGGAAFL
                     AHKSGAPILPIAVIDTENKSVFGHLKKWRRAPVTVRVGKVFRLADQPSARKEALETGT
                     SRIMAALADLLPEKYRGK"
     gene            2789..>3644
                     /locus_tag="PLG52_07720"
     CDS             2789..>3644
                     /locus_tag="PLG52_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41372.1"
                     /translation="MDFILSLLAGIAFPAPPTLAGWLVWVSLLAVGATYLYRQRAAQS
                     AWGSKGWGLFLVLFLLVPILNLFIGLRLTSGSVRPLPGLPADAPGSALMVFSAIPWLL
                     GGGLLGPLAAAILGAFAGLVRGTWDLYSLFPVIEFAFLGVWFARNSRQRYRTPAYRLL
                     RQPLVGALLLIPIHTLFYTLSALFTQWGTEIPATARLDFAISNALVVTLAFSGEMLIG
                     GLVAQVISMTFPASWGSKLPLQPSPGEKSLESRFIFAVGSFILVLLLTLLIGGWVVAG
                     QAARGMLED"
BASE COUNT          699 a          888 c         1036 g         1021 t
ORIGIN      
        1 gccctcgctc tcctcagcgg accgtccgtt tggctgggtc tgttagggat cggcatcact
       61 tatggcattg ccaaactgct cgcccgccgc acagaagaat catcagaaga agaaaacaca
      121 cccacagaaa aacggaagcg agatcgttcg tcttctgcct ttgggaaaga gtttttcaaa
      181 tcttctaacc ttataacttt tgcacttgcc tttatctttg ctggctctct cttctttatt
      241 gtgcccggtg gtattggcgc tgcattttct tccatccctg cttttttcaa aacatgggca
      301 gcgacgggtc ccgttacaca agggatgttg atcatctctt tgtttgtgta tcagcctttt
      361 gctctgttgc tggcaggtat ggctctactc cgcggttgga tgggtggtct gcgccgcatc
      421 gttttcttga gcatttggtt ctttgttgcc ttgttgctgg tgatcttcct gtcaggtcgc
      481 caaatgtacg acctggcttg ggcgctcatt ccattgaatg ccttggctgc tttggaatta
      541 gtacgtcacc ttagcatttt ccctgaagaa cgccgcgaag tagcgggtgt ggttttatta
      601 accacgttca tttgggtatt catctggctt ggcactgcgg gctttagctg gatattggtc
      661 gatacacccg attatcgttt gcgggcaggc atgttggttg gttcgatctt cttgcttatt
      721 atcagtttgg tcttggtggc ggcgggttgg tccattcgca ttgcacggtt gggtggcgtg
      781 tggggcatgg caatctgctt ggggctgttg agtgtaggtg gtttatttgg tgctaccggt
      841 ttacgcggat taagcgcccc cgaactatgg tggcttccta acgtcccaac ccaagcccaa
      901 ttggtgcgag atacggttca tcaggtttca gacattggca gaggcagtat ctattctgcg
      961 acggttgcga tcatcggcgt caactcaccg gcgttggaat gggctgtccg tgaaaatccc
     1021 gtgcaagtgg tcgatgcggt tgacccgaac aatacgcctg attttgtcat cacctcctat
     1081 gaattagacc ctggactggt ctcagcctat cgcggacagg actttacctg gcggcaggct
     1141 cccatttgga atggggcaga cacctttgct tggttgaagt ggatttcgtt gcggcaaatg
     1201 cctcaaagcc aagaagcgat catcctctgg gcacgaaccg acctcttcct cggtggaaca
     1261 cagtaagcac caaagaccgt agaacttcta cggtcttttt ttatagagtg tctgatgtaa
     1321 aatgggggca ctatgacaaa aactcctcct tccattattg ctctcgacgg accagccgcc
     1381 tctggaaaat ctacagtggg gcggcggctc gcagatgcat tgggttattt attctttgat
     1441 acaggtgtga tgtatcgcgc tgtgacgtgg atcgcgctca atcacgatat ggacctaaaa
     1501 aacgaagctg ccattaccca aatggcagag tctgccgagc ttgatattcg cccgccctct
     1561 caaaacgacg gtcgtgcctg cgatattatc gtgggcgata aagacatcac gtgggacgtc
     1621 cgttcgggag atgtggaggc tagtgtctca ttagtggcgg catatgcagg ggtgcgcact
     1681 gcgctctctg gtcaacaacg ccgcattggg ttgcgtggta aggtggtgat ggtcggcaga
     1741 gatattggca cggtcgtttt acccgaagca gatctgaaag tgtatatgga tgccagcgct
     1801 gaagaacgtg ctcgccgtcg ttttaaagat attacttccc gtggcgaaca agctaactat
     1861 gacgagcttc tcaaaaagat gattgagcgt gaccgcttag actccacgcg cgccgtcgct
     1921 ccgctgcgcc ctgccgacga tgcgctggta ttggatacgg ataaattgtc ggaagaagaa
     1981 gtctttgcca agattttaga gatgtgcaaa taaagcatgt ctgatattgt gcagccgcct
     2041 aaacccgtca ctgaaatttg gaagccggaa ttagtgcggc tgcccgagtt gacatctctg
     2101 cgcctcgcct ttcgcaggtt cgcatatgga ttggtcaaac tggttgtatg gagttgcctg
     2161 cgcgtcacac gggaagggtt ggaaaatatc ccgtcaagcg gacctttgct gatcgttatc
     2221 aatcatctag gtcatgccga tgtgccagca ttgatctcgt ccttgcccta tacgcccgat
     2281 gcacttgggg aaattgagtt gtacgacgtc ccggtcttgg gtcggctcat ggattggtat
     2341 ggtgttatct ggctgcaccg tgggcgaccc gataagcgcg ccctgcgtgc cgcactcgat
     2401 ggcttggcag aagggcgtgt catcgtcatt gcacccgagg ggcgttactc gctgactggc
     2461 gcattggaag aaggtacggg cggagcggct tttttggcgc ataaatctgg cgcacccatt
     2521 ttgccgattg cggtgattga tacagagaat aagagcgtct ttgggcatct caaaaaatgg
     2581 cggcgtgcac cggtcacagt gcgggtaggg aaagttttca ggcttgccga ccagccttcg
     2641 gctaggaaag aggcgctgga aacggggacg agtcgcatta tggcggcgct ggcagacctt
     2701 ttgccggaaa agtatcgcgg gaaataattc caaaaatgta aacaggcttg tgaaccacct
     2761 gtggtatttg gggctataat tctaaaaaat ggatttcatc ctttcgctcc ttgcaggcat
     2821 tgcgttcccc gccccgccca ctcttgcagg atggctggta tgggtgtctc tgcttgcggt
     2881 gggggcgacg tacttatatc gtcaacgcgc ggctcaaagt gcatggggct caaaaggatg
     2941 ggggcttttt cttgtcctgt tcctgcttgt tccgatctta aatttattta ttggattaag
     3001 actgacctcc ggctctgtgc gacctttgcc tgggttgccc gccgatgcgc cgggttcggc
     3061 gctgatggtt ttttctgcca ttccgtggct gttgggtggc ggcttgttgg gtccgctggc
     3121 ggctgccatt ctgggggcgt tcgccggttt ggtgcgcggc acttgggact tgtactcgct
     3181 cttcccggtc attgagtttg catttttagg cgtttggttt gctcgcaatt cacgtcaacg
     3241 ctatcgcacc cccgcttatc gcctgcttcg tcagccgttg gtgggggcgt tgcttttaat
     3301 cccgattcat acgttgttct atacattgag tgcgcttttc actcaatggg gcacagagat
     3361 tcctgccacc gcccggctag actttgccat tagcaatgcc ttggtcgtaa cgcttgcctt
     3421 tagcggtgag atgttgatcg gtggtttggt ggctcaagtc atttcgatga ccttccccgc
     3481 cagttggggc agcaaactac cgttacagcc ttcgcccggt gagaagagcc tcgagtctcg
     3541 ttttatcttt gccgtcggga gtttcattct tgttttgttg ctgacactcc tcatcggtgg
     3601 atgggtggtg gcagggcagg cagctcgtgg catgttggaa gatc
//
LOCUS       DAOQNJ010000234         3639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17084_length_3639_cov_8.763114, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000234 DAOQNJ010000000
VERSION     DAOQNJ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17084_length_3639_cov_8.763114"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /gene="ligA"
                     /locus_tag="PLG52_07725"
     CDS             complement(<1..976)
                     /gene="ligA"
                     /locus_tag="PLG52_07725"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNB41373.1"
                     /translation="MADKLLSRYEELKAQINFHNYRYHVLDAPIISDLEYDRLLNELK
                     KIEAEHPDWITPDSPTQRAGAKPSEKFEKVRHPAPILSLANAFGGDDARAWFERVKKL
                     DDRVEKAKFVVEPKIDGLSVVLHYRNGMFVQGATRGDGEVGEDITSNLRTIKAIPLKI
                     PVEGQKSKVKVPSYLVVRGEAFIPIKDFEELNKRLEEAGEKTYLNPRNTAAGSLRQLD
                     PVLTASRPITLLVYQIVYAEGGDVPTSQWEILEYLKSLGFPITDISKRFNTLEPAIAY
                     TETFDKGRDALPYEADGMVIKIDDLSLAADLGFVGKDPRGAIAFKFPAR"
     gene            complement(1041..1376)
                     /locus_tag="PLG52_07730"
     CDS             complement(1041..1376)
                     /locus_tag="PLG52_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41374.1"
                     /translation="MSNQLFEAALKELEARQEDVRPSDVISFPEPLRSAVNFVVRLKR
                     FSLTEFHEKLPEFTREEVKRLADILIKRHLFDLSRFATEAEPYYEARLSALTRPLTRP
                     PSDIWKKID"
     gene            complement(1406..2161)
                     /locus_tag="PLG52_07735"
     CDS             complement(1406..2161)
                     /locus_tag="PLG52_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNB41375.1"
                     /translation="MSKIISVHSFRGGTGKSNTTANIAALLAMDGARVGVVDTDIASP
                     GIHVLFNLDEADMENSLNDYLWGKCTIEQAAQDVTGHIGAEVKGQIFLIPSSIKAGEI
                     ARILREGYDVGLLNDGFRDVIEKLKLDYLLIDTHPGLNEETLLSIAISNALVIIMRPD
                     QQDYQGTAVTVDVAKKLDVPNLFILVNKAPQNLDLEEVRARVEQTYDAKVGAILPHSD
                     EMMMLASSGLFTVHYPEHVVTKGLRKLVDQLKA"
     gene            2323..2889
                     /locus_tag="PLG52_07740"
     CDS             2323..2889
                     /locus_tag="PLG52_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB41376.1"
                     /translation="MTTNYMQNNLVSLQDASDELLIQKYLNGEREAFDALYHRHLPKV
                     YKRVRYVVPPNDVEDVTQEIFIAAVKSLSSFRGDSKFGTWLRTLTNHKVAEFYRKRTR
                     KQEPQLAPLSDAIAQVAGSTSQSMEDRIFLQRALLNLPENYQEILLLRFAEDLQFNEI
                     AELTNQNLEATKSLFRRAVTALRNHLEN"
     gene            2892..3356
                     /locus_tag="PLG52_07745"
     CDS             2892..3356
                     /locus_tag="PLG52_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41377.1"
                     /translation="MDQRNKSIQQDNELADFVDQLLDGKNPPASTSDEELVSLEKTLL
                     RLNDAFPPENLDEAKSKQMLARLKTRMRREEEEKAAKPSFWSRLFDLQANPQVGLLIA
                     AAVVVFVIVSFPAVVQPSGSAVSGTASSGGSMYVGLGLLALLFIVYLVSRKK"
     gene            3411..>3639
                     /locus_tag="PLG52_07750"
     CDS             3411..>3639
                     /locus_tag="PLG52_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41378.1"
                     /translation="MKKNNYKWGRLFFLIVALLTIFSLLLSACAPAQGLEGGAGGNGG
                     GNGGGGGNAGGNSGGGNGGGNGNAGGNGGGGG"
BASE COUNT          911 a          881 c          917 g          930 t
ORIGIN      
        1 cgcgggctgg gaacttgaag gcgatggctc cgcgcgggtc tttgccgaca aagcccaagt
       61 ctgcggcgag ggataaatcg tcgattttta tgaccatgcc atcggcttcg taaggaagcg
      121 cgtctcgtcc tttgtcaaag gtttcggtat aggcgatggc tggctccaag gtgttgaagc
      181 gtttggaaat atccgtgatg ggaaacccca gcgatttgag atattccaaa atttcccatt
      241 gcgaggtggg aacatcaccc ccttcggcgt acacaatctg atacacaagc aatgtgatcg
      301 ggcgagatgc agtcaacacc gggtcaagct gacgcagcga cccagctgcc gtgttgcgcg
      361 gattgagata ggttttttcg ccagcttctt cgagacgttt gtttaattct tcaaagtctt
      421 tgatcgggat gaatgcctcg ccgcgtacga caagatagga aggtacctta actttggact
      481 tttgcccttc caccggaatt tttagtggaa tggctttaat agtgcgcaag ttgctggtga
      541 tatcttcccc tacttcgccg tcgccgcggg tggctccctg cacgaacatg ccattgcgat
      601 aatgcagcac aaccgacaag ccgtcgatct tcggctcaac cacgaacttt gccttttcta
      661 cacggtcatc taattttttg acacgttcaa accaggctcg tgcgtcatca ccgccaaagg
      721 cattggcaag tgaaaggatg ggtgccgggt ggcgcacctt ctcaaatttt tctgaaggct
      781 tcgccccggc acgttgagtg ggggagtcgg gagtgatcca atcggggtgt tctgcctcga
      841 tcttttttag ttcgttgagt aggcggtcat attcgaggtc actgataata ggcgcatcca
      901 acacgtgata ccgataatta tggaagttga tctgcgcttt aagttcttca tatcgtgaga
      961 gaagtttatc tgccattttt tgattatacc cagtgcagag aaaaataaaa cccgcctgtt
     1021 ttatgcaggc gggtgagaaa tcaatcgatt ttcttccaaa tgtctgatgg cgggcgggtc
     1081 agcggacggg tcaatgcaga gaggcgagct tcgtagtagg gttccgcttc ggtggcaaac
     1141 cgtgaaaggt caaacaggtg gcgcttgatc aaaatatctg ccaggcgttt cacttcttca
     1201 cgggtaaatt ctggcagctt ttcgtgaaat tccgtcaggc tgaagcgttt caaccgcacg
     1261 acgaaattca ctgcagaacg cagcggttca gggaacgaga tcacgtccga aggacgaaca
     1321 tcttcctgac gtgcttccaa ttccttaagc gcagcttcaa aaagttggtt gctcatggcg
     1381 ccttcccttc acctaggcga atgccctagg ctttgagttg atccaccagt ttgcgcaagc
     1441 ctttggtcac cacatgttca ggataatgaa cggtgaagag accagaactg gcgagcatca
     1501 tcatttcatc tgagtgcggg agaatagctc ccactttcgc gtcgtaggtc tgctccacgc
     1561 gggcgcggac ctcttcaagg tccagattct gaggcgcttt gttgacgaga ataaagaggt
     1621 tgggaacatc gagcttcttc gcaacatcca cggtcactgc tgtgccctga taatcttgct
     1681 ggtctgggcg catgatgatg accaaggcgt tggagatggc aatggagagc aaggtctctt
     1741 cgtttaagcc ggggtgggta tcaataagaa gataatccag cttcaacttt tcgatcacat
     1801 cgcggaagcc gtcgttcaac agacccacgt cgtaaccttc gcgcaaaatg cgggcgattt
     1861 cgcctgcttt gatgctggag gggatgagga aaatctgacc cttaacctcg gcgccgatat
     1921 gtccagtcac gtcttgagcg gcttgttcga tggtgcactt accccacaaa tagtcattga
     1981 gggagttttc catatccgct tcatctaaat tgaagagaac atgaataccg ggcgaggcaa
     2041 tgtcagtatc cactacacca acacgtgcac catccatggc gagcaaagca gcaatatttg
     2101 ctgtggtatt cgatttaccc gtgccgccgc ggaaggaatg aactgaaatg attttggaca
     2161 tgcactgtct ccgattctat gatagtcttt cgaaatctct gaaaatcagt tcgataatta
     2221 tagcctaact atattgaaaa tcaaatccct acttttcatt tctcagttat gttatagtac
     2281 ctttctaaac tgaaagacga aaacgaccca ttttggcgct tcatgacgac aaattatatg
     2341 caaaacaacc tagtatccct gcaggacgcc agcgacgaac ttctcatcca gaaatacctt
     2401 aatggagaac gagaagcttt cgatgctctt tatcaccgcc acctccccaa ggtctacaaa
     2461 cgggtcaggt atgtggttcc gcccaacgat gtagaggatg tgacgcagga gatctttatt
     2521 gctgcggtaa agtccctttc ctcctttcga ggtgactcga aatttggcac atggttgcgc
     2581 acccttacca accacaaagt ggcagagttc tatcgaaaac gcacccgcaa gcaggagccg
     2641 caacttgcac cattgtcaga cgcaattgcc caagtcgctg gtagcacgtc ccaatcgatg
     2701 gaagatcgca tcttcctcca acgagcgctc ctaaacctgc cagagaacta tcaggaaatc
     2761 ttgctgctgc gttttgccga agatctacag ttcaacgaga ttgctgaatt gaccaaccaa
     2821 aatcttgaag ctaccaaatc tttgtttcgt cgagccgtta ccgcacttcg caaccacctg
     2881 gaaaattaac catggatcaa agaaacaaat ctatacaaca agataacgaa ctcgcggact
     2941 ttgtcgacca attgttggat gggaaaaacc ctcccgcctc aacctcagac gaggaactgg
     3001 tcagcctcga gaaaacgctt ctccgtttga acgatgcctt ccctccagaa aacctggacg
     3061 aagcaaaatc caagcaaatg cttgcccgtc taaagactcg tatgcgccgg gaagaagagg
     3121 aaaaagcagc caagccttcc ttttggagca ggctttttga tcttcaagcc aacccccaag
     3181 tcggtttgtt gatcgcagca gcagtagttg tttttgtgat tgttagtttt cctgctgtgg
     3241 ttcaaccctc aggttctgcc gtctctggaa cagcatcttc cggtggcagt atgtatgtcg
     3301 gtttgggatt gctagccttg ctgttcattg tctatttggt ttctcgcaag aaataatagt
     3361 caaaataaaa acgccaaacc aatgtattta tttgtatcaa ggagagatca atgaaaaaaa
     3421 ataattacaa atggggcaga ttgttttttc ttatcgtcgc tttgctgacg atcttttcac
     3481 tgcttttatc tgcttgtgcc cctgctcagg gattagaagg cggcgctggt ggcaatggcg
     3541 gcggaaatgg cggcgggggc ggtaacgctg gtggaaatag tgggggtgga aacggtggcg
     3601 gtaatggcaa tgccggcgga aacggcggcg gaggtgggg
//
LOCUS       DAOQNJ010000235         3632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17135_length_3632_cov_9.733576, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000235 DAOQNJ010000000
VERSION     DAOQNJ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17135_length_3632_cov_9.733576"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..518
                     /locus_tag="PLG52_07755"
     CDS             186..518
                     /locus_tag="PLG52_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41379.1"
                     /translation="MKLSLKHYGIILGALLAAYFHLILFPDIGFTLNGLGYLGLLGAY
                     FLPIAFFQQKHKMVWWGLVGYTILTIVLWAIMGNKQFVAGTTSAIGYYAKAAEVFLLA
                     CLWADKPQ"
     gene            618..983
                     /locus_tag="PLG52_07760"
     CDS             618..983
                     /locus_tag="PLG52_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41380.1"
                     /translation="MEWHIEYIPEIALLYVKTKGILTNEKANQMVAEIALAMKEHQCL
                     CQIVDHRETVFDFRTLDYYERPKVNQQLGMSKQWRVAMIFKQLSEETLFMETVFRNRG
                     FEFRQFDDLDKAKEWVLKK"
     gene            complement(991..1701)
                     /gene="rsmG"
                     /locus_tag="PLG52_07765"
     CDS             complement(991..1701)
                     /gene="rsmG"
                     /locus_tag="PLG52_07765"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNB41381.1"
                     /translation="MDSLIQDAKTLFNVHLTARQVVALVNYEKELMEWNQKFNLTAIR
                     DVESIRTKHFLDSYSCVLAWKANPPQRLIDVGTGAGFPGIPLKIIYPTMQVTLVESVG
                     KKAMFCQHVIQTLGLEGIEVLHSRAEDVGQNPAHRETYDWAVARAVANLNILSEYLLP
                     LVKLGGTMLAQKGETGPVEAQAAEKAMKILGGKLKQLIPVNLPGVVDDRYLVLTDKVA
                     ATPPKYPRKAGIPMKTPL"
     gene            complement(1761..2741)
                     /locus_tag="PLG52_07770"
     CDS             complement(1761..2741)
                     /locus_tag="PLG52_07770"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNB41382.1"
                     /translation="MKRLTPYSLILIAMIVLGACASAPRAVELSGGSAPASEPYYDQS
                     SVMPAPTMMYATDGIIAEEAQVSGEGSGNANAAQVERIVIQNADIAVVVSDVELRMDE
                     IQKMAVDLGGFVVSSNLYQSYTSDYTPVPEASLTIRVPAENLDEALEKIKKDAVEVQN
                     ETRSGQDVTAEYVDLKSRLKTYQAAEEELTQLMDNAQNSDEVVNIFNQLMYYREQIEI
                     IQGQIKYYEEAAALSAISIRLIAEETTKPVTIGKWEPKGVALEAIQDLLDFWKGFVDF
                     MIRFVIFTLPMLITIGIPFYLAFIGVRWVFRKMRGNKKKVEPQVKEEEKK"
     gene            complement(2879..>3632)
                     /locus_tag="PLG52_07775"
     CDS             complement(2879..>3632)
                     /locus_tag="PLG52_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNB41383.1"
                     /translation="LRGCWNNMRILVMSDIHANYTALEAVLKDAGSVDETWCLGDLVG
                     YGPDPNAVVEEVQEIKNLTCLMGNHDVAVVGRMSLETFNGDAKRSLMYHEKVLTASNI
                     DFIKSLPSKTKVRGEASMAHGSPRDPLWEYILNSMTASLNFEHFDTPWCFVGHTHIQS
                     SFSQVDASGRVLVEQTRPDVTLQLQPKLILNPGSVGQPRDRDPRAAYAIYDTEARTWT
                     PKRVMYNIAEVQERIRAAGLPEKHAVRIGEGW"
BASE COUNT          901 a          866 c          860 g         1005 t
ORIGIN      
        1 gggttaaagg tgcccgccga atttcgacgg gaaacaattg aaacgtttgt attgtactct
       61 ttcttgcttt atttgttaat caaattgggt tttatcgtga aaataaacgc atgtaagaac
      121 ccatggatgt gtgatgtcca tgggttaagt ctctgtcgta attccaattg ccaaaggagt
      181 aaagcatgaa actaagtcta aagcattacg ggatcattct gggggcattg ttagcggcat
      241 acttccacct tattctcttc cccgatatcg gcttcaccct gaacggatta ggatatctag
      301 ggctgctcgg agcttatttt cttccaatag ccttctttca gcaaaaacat aaaatggtat
      361 ggtggggttt ggtcggttat accattctca ccatcgtgct atgggcaatt atgggaaaca
      421 aacaattcgt tgcagggacg accagtgcca ttggatacta tgccaaggcg gctgaggttt
      481 tccttttagc gtgtctttgg gcagataaac cccagtaatc tttttgcgtt atctggtaaa
      541 atccgccgct attgtagtag ttcataacat ggtatgactt tattgtcata ccatgcttct
      601 ttttaggcgg tagccgtatg gaatggcata ttgaatatat ccccgagatc gcccttttat
      661 atgtaaagac caaggggata cttacaaatg aaaaagcaaa tcagatggtt gctgagattg
      721 cgcttgcaat gaaagaacat caatgtctct gtcagatcgt ggatcatcgt gagacggttt
      781 ttgatttcag aacccttgat tattatgaac gtccaaaagt aaaccaacag ttggggatgt
      841 ctaaacagtg gcgtgtcgcc atgatattca aacaactctc tgaagaaacc ttgtttatgg
      901 aaactgtttt tcggaatcgt ggatttgagt ttcgtcagtt cgatgatttg gataaagcca
      961 aagagtgggt tttgaaaaaa tagacgcaag ctataaaggc gtcttcatgg ggatgcctgc
     1021 cttgcgggga tacttcggtg gtgtagcagc gactttatct gttagaacca gataacgatc
     1081 gtctactaca ccgggcaagt tgacggggat caattgcttt aattttccac ctaaaatttt
     1141 catcgctttc tcggcagcct gtgcttcaac gggtccggtc tcgccttttt gggcaagcat
     1201 cgtcccgccg agtttgacaa gcggcagaag atattcgctc aagatgttga gattcgccac
     1261 agcccgtgcc acagcccagt cgtaggtttc acgatgagcg gggttttgac ctacatcttc
     1321 ggcacgagaa tgcaagactt cgatgccttc caagcccagc gtttgaatca catgctgaca
     1381 gaacatggct ttcttgccga ctgattcaac caaggtgact tgcatggtag ggtaaataat
     1441 cttaagcgga atgccgggga aaccagcgcc cgtgcccacg tcgatgaggc gttgcggcgg
     1501 gttggctttc cacgccagca cacaggaata tgaatccaga aaatgcttgg tacgaataga
     1561 ctctacatcc cgaatggcag taaggttgaa tttttggttc cactccatca attctttttc
     1621 gtaattgacg agcgccacca cctgtcgcgc cgtgaggtgg acgttgaata atgtttttgc
     1681 gtcttgaatc agagagtcca tatgcgaaga ttataaaaca aagaggacgg cggtacaccg
     1741 tcctctcgtg ttatgacgtt ttacttcttt tcttcttcct tcacctgcgg ttcaactttt
     1801 ttcttgttgc cacgcatctt acggaagacc cagcggacgc cgatgaatgc caggtagaac
     1861 ggaataccga tggtgatcaa catgggcaga gtaaagatta cgaagcggat catgaaatcg
     1921 acgaagcctt tccagaaatc gagcaggtct tgtatggctt cgagcgcaac gcccttcggt
     1981 tcccatttgc caatcgttac tggtttggtg gtctcttcgg cgatgagacg aatgctaatg
     2041 gcagagagag cggcagcttc ttcgtaatac ttaatctgac cctgaatgat ctcgatctgt
     2101 tcgcggtagt acattaactg attgaagata ttgacgacct catctgagtt ttgtgcattg
     2161 tccatcaatt gggtcagttc ttcttcagcg gcttgatagg tcttgaggcg ggactttaag
     2221 tccacgtatt cagcggtgac atcttgccca gagcgggttt cgttctgaac ttccaccgca
     2281 tctttcttga tcttttccag cgcttcatcc agattttcag caggcacgcg gatggtcagg
     2341 ctggcttcag gcaccggggt gtaatcactg gtatagctct gatacaaatt ggaagacact
     2401 acgaatccgc caagatcgac tgccattttt tggatctcat ccatacgcag ttcgacatct
     2461 gatacgacga ctgcaatatc tgcattctga atgacaatac gttccacttg agcggcattg
     2521 gcgttcccgc tgccttctcc acttacttgg gcttcttcag cgataatgcc atcagtagca
     2581 tacatcatgg tcggtgcggg cattacagag gattggtcat aatagggctc cgaagctggg
     2641 gcagacccgc cagaaagttc cacggcgcgc ggagcactgg cacaagctcc aagaacgatc
     2701 attgcaatca aaataagact atagggcgtt aggcgtttca tttctttctc cttcttttcc
     2761 tttggtaatt tctctcttca aaggacgcta cgctaaaata aattgttccc acgtattgta
     2821 caacttccta taaagaaaaa gccctcccaa acttcaggga ggacttttgt gggaggcgtt
     2881 accaaccttc gccaattctc acggcatgtt tttctggcaa tcccgccgca cggatgcgct
     2941 cttgcacttc ggcgatgttg tacatcacac gttttggcgt ccaagtgcgg gcttcggtgt
     3001 catagatcgc gtaagcggca cgcgggtcac ggtctcgggg ttgaccaaca ctgccggggt
     3061 tcagaatcag tttcggctga agttgaagcg tcacatcggg tctggtctgc tcaactagca
     3121 cacgtcctga agcatctact tgagaaaaac tactttggat gtgggtatgc cccacaaagc
     3181 accaaggggt atcgaaatgt tcgaagttca aactcgcggt catcgagttg aggatgtatt
     3241 cccacaacgg gtcacgcgga ctgccatgcg ccatagacgc ttctccgcgc accttggttt
     3301 tgctcggcag agacttaatg aaatcaatat tgctggcggt aagcactttc tcgtgataca
     3361 tcaacgaacg cttggcatcg ccgttgaatg tctctaacga catgcgccca acaaccgcca
     3421 catcatgatt gcccatcagg caagtcaggt tcttgatctc ctgcacttct tccaccaccg
     3481 cgtttgggtc cggtccatat ccaacaagat caccaaggca ccacgtttca tccacagacc
     3541 ccgcatcttt taatacagct tccaacgccg tatagttcgc atgaatatcc gacatgacca
     3601 aaatacgcat attattccaa cagcctcgca at
//
LOCUS       DAOQNJ010000236         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17139_length_3631_cov_8.839206, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000236 DAOQNJ010000000
VERSION     DAOQNJ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17139_length_3631_cov_8.839206"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLG52_07780"
     CDS             <1..594
                     /locus_tag="PLG52_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNB41384.1"
                     /translation="MYSELSYGIKTDMAFEDALKLAVLGKDISIESIKTGVIDPQQGM
                     AIFDNTTLNGEAASILKPVMDKIRVLRDEIFTSTGPTSPLAVPNGYTDPNLFAQAMQQ
                     EEARVRVMDGTFMPGLDQRAGAVFQSYGMNVTEVAPSPEAYGQTIVIVYGPKLYTIKW
                     LQATFGISDNQIRFSPDPNQTVDIEIRIGSDIAGTIP"
     gene            complement(631..1071)
                     /locus_tag="PLG52_07785"
     CDS             complement(631..1071)
                     /locus_tag="PLG52_07785"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNB41385.1"
                     /translation="MMNEQIPQQPLAPLSPAEERQWAMFAHLGVLTNLFSGFLGPIVP
                     LAIYMIYKDRSRYVAYQSLQALIFQLIWWVGGGLLTGIVWAITGTLSAFIIGLICIPF
                     ACIISAMPLVALGYGTYAGIQCNQGQDFKYWLIGDWLRTTLTDN"
     gene            complement(1183..1488)
                     /locus_tag="PLG52_07790"
     CDS             complement(1183..1488)
                     /locus_tag="PLG52_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41386.1"
                     /translation="MSQAPMMEVSSDDKLWAALAYAFSPLIPIILMFMEDKKNRPFIK
                     AHNMQALILGIITVVTSGFCIGILVWIYALYCAYQAYQGLEVKIPVLSDFVKNQGWA"
     gene            1650..2567
                     /gene="fmt"
                     /locus_tag="PLG52_07795"
     CDS             1650..2567
                     /gene="fmt"
                     /locus_tag="PLG52_07795"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNB41387.1"
                     /translation="MSIRVVFMGSPDFALPSLRALVDRYNVVGVVTQPDRASGRGREL
                     KAPPVKLLAQDLNIPVMQPQKLREPEAMQQLQAWNPDLIVVAAFGQILRKDVLDLPKY
                     GCVNVHASLLPRWRGAAPINASVLAGDEETGVTIMKMDVGLDTGPMLSMEKIRIKPDD
                     TAGALFEALSTLGAKLLIETLPGYINGTVLPQPQPEEGATYAPMLKKEDGRLDFNQPA
                     VELERRIRAMNPWPGAWFEWNGNILKVSRGAVSEAKGKEVGSRLIVEGRPAVHCADGV
                     LILEEVQPPSKKVMPGKAFLSGTRNWESS"
     gene            2619..3368
                     /locus_tag="PLG52_07800"
     CDS             2619..3368
                     /locus_tag="PLG52_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HNB41388.1"
                     /translation="MFNWKVFLAEFIGTFALVFIGAGAGVVQAGLVGVALAHGLTLMV
                     FAYSFGHISGTHVNPAVTFGLALQGVVKWGEAVVYWVAQCLGAISAAGLLHLVVSQLG
                     GTINAGATVGALTDKFPVYAMLIEAVLTFFLLNTILHTAVAGKGGQFAGLAIGLTLTA
                     AILVGGPMTGASLNPARTLGTAVYTSPSLANVNTYVTYLFGPLIGATAAVLIFNVFKT
                     ETPVAEEEISVPVEEPAKPATKRKTTAKAKK"
     gene            3446..>3631
                     /locus_tag="PLG52_07805"
     CDS             3446..>3631
                     /locus_tag="PLG52_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNB41389.1"
                     /translation="MTQYVGAIDQGTTSTRFIIFDHNGNVVAVDQKEHRQVYPKPGWV
                     EHDALEIWQRTQEVMAGA"
BASE COUNT          969 a          788 c          922 g          952 t
ORIGIN      
        1 atgtatagtg aactttcata cggtatcaag acagatatgg catttgaaga tgctttgaag
       61 cttgctgtgc ttggcaaaga tatttctatt gaaagcataa aaacaggtgt tattgaccct
      121 caacaaggta tggcgatctt tgataacaca accctcaatg gggaagctgc cagcattctc
      181 aaacctgtga tggataagat tcgcgttttg cgtgatgaga tttttacttc tacaggtcct
      241 accagcccat tggcggttcc gaatggatat acagacccga atctttttgc tcaagctatg
      301 cagcaagaag aagcccgcgt ccgtgttatg gatggtacct ttatgcccgg gcttgaccaa
      361 cgtgccggag cggtattcca atcctatggc atgaacgtca ccgaagtggc tccatcccct
      421 gaagcgtatg gacaaacaat agtgattgtg tatggaccaa agctatatac gatcaaatgg
      481 ttgcaagcga cctttggaat ttcagataat cagattcgct tcagcccaga cccgaaccag
      541 actgtagata ttgagatccg aataggttct gatattgcag ggacaatccc gtaatataaa
      601 aaatcccctt cttttgaagg ggatttttta ttagttatcc gttaatgtgg tgcggagcca
      661 atctccgata agccaatact tgaaatcttg accctgattg cattgaatcc ctgcgtaggt
      721 tccatacccg agggcgacca atggcatagc gctgatgata caggcaaacg gaatacagat
      781 tagcccgatg ataaaggcgc tcaaagtgcc ggttattgcc cacacaatac ctgtcaataa
      841 accgccaccg acccaccaaa tgagttggaa aatcaacgct tgaagcgatt gatatgcaac
      901 atagcgggaa cgatctttat agatcatata aattgctaat ggcacaatag gacctaaaaa
      961 acctgaaaat agatttgtca acactcctaa gtgggcgaac atcgcccatt ggcgctcttc
     1021 tgcagggctg agcggggcaa gtggttgttg aggtatttgt tcgttcatca tattctccta
     1081 atagtataca aactctttga attaatacgt gtataggaga ggcaaagtag caaaataaaa
     1141 gacccgcgtg ttacacgcgg gtcttttgta gagcgagact gattatgccc agccctgatt
     1201 cttgacgaaa tcgctaagaa cagggatctt aacttccaag ccttggtatg cctggtacgc
     1261 gcaatacaga gcgtatatcc agaccaaaat accaatgcag aaaccggaag tgacaacggt
     1321 gataatgcca aggatcaaag cctgcatatt gtgagctttg atgaatggac gattcttctt
     1381 atcttccatg aacattaaaa tgatcgggat aaggggggag aaggcgtaag caagagctgc
     1441 ccacagctta tcatcactcg atacttccat cataggtgct tgagacatgg tttattcctc
     1501 cgaataagtt gtttgaattt taagtatttt acaaccaatc tacacaacgt gcaaccaaac
     1561 taattcaccc aatggtactg atacgttacg aagagcccgt tttgctgaaa attctcctta
     1621 attattgaga ccagcggtaa aatctgcata tgtctattcg tgttgttttc atgggttcgc
     1681 ccgattttgc tcttccgagc ctccgcgctc ttgttgatcg ctataatgtt gttggggtgg
     1741 ttacccagcc agatcgtgcc tctgggcgcg gacgtgaact gaaggcgccg cctgttaagc
     1801 ttttagcgca agacctcaat atccccgtca tgcagcctca aaaattacgt gagcctgaag
     1861 cgatgcaaca gttgcaagct tggaatcctg acttgattgt ggttgcagct tttggtcaaa
     1921 tcttaagaaa agatgtatta gacctgccca aatacggctg tgtcaatgtg catgcttcac
     1981 tgttgccacg ttggcgtgga gcggcaccga ttaatgcatc tgtgctggct ggagacgaag
     2041 agacgggcgt taccattatg aaaatggatg tggggttgga tacaggtccc atgctttcga
     2101 tggaaaaaat tcgcatcaag ccagacgata cagcgggcgc actcttcgag gctttatcta
     2161 cgctgggagc gaaacttctt attgagaccc tgcctgggta cataaatggc actgtcttgc
     2221 cacaacctca acccgaagaa ggtgcaacct atgccccgat gctcaaaaaa gaagatggac
     2281 gattagactt taatcaacca gctgtggaat tggagcgaag aattcgtgcg atgaacccat
     2341 ggcccggagc atggtttgag tggaacggga acatactcaa agtaagtaga ggtgctgtca
     2401 gcgaagcaaa aggcaaagaa gtggggagca ggcttatcgt tgaaggaaga cccgcagtac
     2461 actgtgcgga tggagtctta attttggagg aagtccaacc acctagcaag aaggttatgc
     2521 cggggaaggc gtttttatcc ggcacacgga attgggaatc gtcgtagcaa attgacaggt
     2581 atggggttat aacttccaaa ttaatctttg gaggatgtat gttcaattgg aaagtatttc
     2641 ttgctgaatt tatcggcacg tttgcgttgg tgtttatcgg tgcaggggcg ggcgtggtac
     2701 aggcggggct tgtaggcgtg gctcttgcac atggtttgac cttgatggtc ttcgcttatt
     2761 catttggtca tatttcgggt acccacgtga acccagcggt gacctttggg ttggcattgc
     2821 agggtgttgt gaaatggggc gaagcggtgg tgtattgggt agctcaatgc cttggggcga
     2881 tctcggcggc tggattgctt catttggttg tctctcaact cggcgggacg atcaatgctg
     2941 gagctacggt aggcgcattg accgataaat tcccggtgta tgccatgttg atcgaagccg
     3001 tgttgacatt cttcctgctc aacacaattt tgcatacggc tgtagcaggc aagggcgggc
     3061 agtttgctgg gttggcaatc ggcttgacat tgaccgctgc cattcttgta ggtggaccga
     3121 tgaccggtgc atctcttaac ccggcacgca ctttaggaac cgctgtttat acaagcccaa
     3181 gcttggcgaa cgtgaatacc tatgtcacat acttgttcgg tccgttgatt ggtgccacgg
     3241 cggctgtttt gatattcaac gtatttaaga ctgagacacc tgtagccgaa gaagagattt
     3301 ctgttccggt tgaagaacca gcaaaaccgg cgacaaagcg caagacaact gcaaaagcaa
     3361 agaaataaat tttgtgcttc tttcaagcct tcgccttcaa agcgaaggct ttttgtttta
     3421 caattaatcc atcggaggca ctctcatgac tcaatacgtt ggcgcgattg atcaaggcac
     3481 gaccagtact cgttttatta ttttcgacca caacggcaat gtggttgcag tggatcaaaa
     3541 agaacacagg caggtctatc ccaagccggg ctgggtggaa catgatgcct tggagatctg
     3601 gcagcgcacg caggaagtga tggctggcgc t
//
LOCUS       DAOQNJ010000237         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17180_length_3626_cov_10.355363, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000237 DAOQNJ010000000
VERSION     DAOQNJ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17180_length_3626_cov_10.355363"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="PLG52_07810"
     CDS             complement(<1..1733)
                     /locus_tag="PLG52_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB41390.1"
                     /translation="MNEVNLDINNKSKTAIQRLKAENARLQADLETLMRERRTMAETS
                     LRQSQELDLLGRVRAAIATKLELPALLHSVVESIVEICGYAYVSIYLLEGETLILQHQ
                     AGYQKVIEKISIHEGISGRVARTGQPILIEDAKGEPEFLQASKKIRSEITVPLMDNGQ
                     VTGTLSIESTSKLTDADLRIMMALGAEINVAIERSRLYEDLQKRNRTLSALQEATLTI
                     IGKLGLPDAVQTILAQTAQLLSTSHGYVYLVQPNGTLEMVTGLGVYPKYIGLTLESGE
                     GLAGKVWETATPLTVNDYIHWDGRSSKFNEHSFHAVMGAPLLHNFKVIGVIGLSHLDA
                     GRTFSKSDEDLLIQLAQLASIAIDNAALYTQINEELVERKQADERLRKSDQRLQVFFN
                     QSLDGFFFCDFEEPIPWKHALNKDETLEYIISTKPFTDVNDAMLKQYGVDRDTFLRLT
                     TKDVFAHDLHQGRNLRRQLFDKGYLHLETNERTLEGNPIWFEGDYVCLYDSLGRITGF
                     FGIQRDITERKQAESELKRHVQNTLTMYELSQNIHTSLNLDLVYQETHKAVQKLMPCD
                     AFLIALLDKKN"
     gene            complement(2041..>3626)
                     /locus_tag="PLG52_07815"
     CDS             complement(2041..>3626)
                     /locus_tag="PLG52_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB41391.1"
                     /translation="RDCLASLDLIDVSGEVMVLVVATDITERRKAEEQLRGSEERFRL
                     MAENIEEGFWITNPNSDEEIYLSPAVERIFGRSMNDLMARSSSFIESILPEDVPLVLT
                     NMERQKLGTATNMEYRIKRPDGRIRWVWDRAFPILNPNGTVRLVTGITTDITERKATD
                     AKIQTQIKRLSALNSIDRAISSSMRMDVALDVLLIEVVAQLNVDAASILLLNETDQTL
                     DFAAGKGFNTSIEHASVPLGFELAGKVGTERKVIHVPNLSELKKGVKRKELIQEEHFL
                     EYFGVPLIVKGQLKGVLEIFNREALNPDPEWLTYLETLGGQAAIAIESAQLFEGLQRS
                     NQELFMAYDATIAGWSHAMDLRDKETEGHTQRVTELTVRLAESMGISQQQRIHIRRGA
                     LLHDIGKLGVPDHILLKPDKLTDEEWHIMRMHPVYARDMLTPINYLRPSLDIPYCHHE
                     KWDGTGYPRGLKGEDIPLAARLFAIIDVWDALRSDRPYRAGWSAEKTRDYIQSESGKH
                     FDPKVVEYFLALLEELSKS"
BASE COUNT          858 a          830 c          858 g         1080 t
ORIGIN      
        1 tgattctttt tatccaataa agcgattaag aaggcgtcac aaggcatgag cttttgaacc
       61 gctttgtgcg tttcttgata gaccaaatcc aggtttaggc tcgtgtggat attttggctg
      121 agttcgtaca tggtgagcgt gttttgaaca tgacgcttca attcactttc agcctgtttg
      181 cgctccgtga tatctctttg aatgccaaag aaaccagtga tacgcccaag cgagtcatat
      241 aaacatacgt aatcgccttc gaaccaaatt ggattgcctt ctaatgtgcg ttcgttggtt
      301 tccagatgca gatacccttt gtcgaataat tgcctgcgta gattgcgtcc ttgatgtagg
      361 tcgtgggcaa agacatcttt ggtcgttaag cgcaggaagg tatcccggtc aacgccatat
      421 tgtttcaaca tggcatcgtt gacatcggta aatggctttg tcgaaataat gtattcgagg
      481 gtttcgtctt tgttgagcgc atgcttccac ggaattggct cttcaaaatc gcagaagaag
      541 aagccatcca acgattggtt gaaaaacacc tgtagacgtt ggtcgctttt tcgcaggcgt
      601 tcatccgctt gctttcgttc caccagttct tcgttgatct gagtgtataa agccgcatta
      661 tcgatggcga tcgatgccaa ctgagccagt tgaattaata aatcttcatc gctttttgag
      721 aaggtccgcc cggcatctaa atgcgaaaga ccaatgaccc cgatcacttt gaagttatgc
      781 agcagtggag ctcccatgac ggcatggaag gaatgttcat taaatttgga agagcgacca
      841 tcccaatgaa tataatcatt tacagtgagt ggcgttgctg tctcccatac cttgccagcc
      901 aacccttcgc ctgattccaa ggtcaagcca atgtattttg gatatacgcc cagcccggtt
      961 accatctcaa gtgtgccatt aggctgaacc agataaacat agccatgtga ggtgctcaac
     1021 aattgagcgg tttgagccaa aatggtttga accgcatcgg gcagccctaa cttgcctatg
     1081 atagtaagcg ttgcttcttg taaggcagat aaagtgcggt tgcgtttttg caggtcttca
     1141 tataaccgtg aacgctcaat cgctacattg atttcagcgc ctaatgccat catgatccgt
     1201 agatcggcat cggtgagttt ggacgtactt tctatgctca acgtgccggt cacttgaccg
     1261 ttgtccatta atggcaccgt gatttcggag cgtatttttt ttgaggcttg aagaaattcc
     1321 ggttcgcctt ttgcatcttc tataagaatg ggttgaccag ttcgggcgac acgtccgcta
     1381 atgccttcgt ggatgctgat cttttcgatg accttttgat accctgcttg atgctgaagg
     1441 attaaagttt caccttctaa tagataaatg ctgacataag cgtagccaca tatttctaca
     1501 atcgattcga cgacactgtg taaaagtgct ggcagttcca attttgtagc aatggcggca
     1561 cgaactctcc ccaataaatc taattcttga gattgtctca aagaagtttc tgccatcgtg
     1621 cggcgttcac gcattaacgt ttcaaggtct gcttgcagac gggcattctc agcttttagg
     1681 cgctgaattg ctgttttgga tttgttgtta atatcgaggt ttacttcgtt catgatgtga
     1741 tgatgagcag atggtgaatt tgctctgctc gtttttattt gtctcttctg cccagtttcc
     1801 ccttttttat tcttgggcat tgctttagag atggcggtca ttccattatt tcctctactc
     1861 cacaagtctc tggggcttaa aatcagtata gcatacccac tatgttagat gaaattacaa
     1921 tatgattgct ctctttgctg atgctgcaat gttataaatc tcaaaaatgg gtaacatcta
     1981 ctcctaatag aaaaagacaa cccttgtgat accacaaggg ttgtcttttt tgttgaattg
     2041 tcagcttttt gaaagttctt ccagaagtgc caggaagtat tcaacgactt ttgggtcaaa
     2101 atgtttgcca gattcagatt ggatgtagtc gcgagtcttt tcggcagacc agcccgcgcg
     2161 gtagggacga tccgagcgga gcgcatccca cacatctata atcgcaaaga gcctggctgc
     2221 cagcgggata tcctcacctt ttaatccgcg tggatagcct gtgccatccc atttttcgtg
     2281 atggcaataa gggatatcaa gcgacggtcg cagatagttg ataggggtga gcatatcgcg
     2341 cgcgtaaacg gggtgcatgc gcatgatgtg ccattcttca tcggtgagtt tatctggctt
     2401 gagcagaata tgatctggga cacccagctt gccaatgtca tggaggaggg ctccgcgtct
     2461 tatatggatc cgttgctgtt gactgattcc catgctttct gctaagcgga cggtgagttc
     2521 tgtgacgcgc tgcgtatgac cttcggtttc tttgtcacgc aaatccatgg catgagacca
     2581 acctgcgatg gtggcatcgt atgccataaa aagttcctgg ttggagcgtt gcaacccttc
     2641 aaatagttgt gcgctttcaa tagcaatggc agcttgcccg cctagtgttt caagataggt
     2701 taaccattca gggtctgggt tcaaggcttc gcggttgaat atctcaagca cgcctttgag
     2761 ttgtcctttt acgatcaagg gcacaccaaa gtattccagg aaatgctctt cttggataag
     2821 ctctttcctt ttgacccctt tttttaattc agacagattg ggcacatgaa ttacttttcg
     2881 ttcagtgcca actttaccag ccagttcaaa cccgagcggc acacttgcat gttcgatgga
     2941 agtattaaac cctttgcctg ctgcgaagtc taaggtttga tcggtttcgt tcaaaagaag
     3001 gatggaagca gcatccacat taagctgggc gacaacttca ataaggagaa catccagagc
     3061 tacatccatg cgcatgctgg agctgattgc ccggtctatg gagttgaggg cgctgagacg
     3121 cttgatctgc gtttgaattt tggcgtcggt tgccttgcgt tctgtgatgt cggtggtaat
     3181 acccgtcaca agccgcaccg ttccattcgg gtttaggatc gggaaggcac gatcccagac
     3241 ccagcggatg cgaccatctg gacgtttgat gcggtattcc atattggtgg cggtacccag
     3301 tttttgtctt tccatattcg ttagaaccag cggcacatct tctggcagaa tgctttctat
     3361 aaacgaggaa cttctcgcca taaggtcatt catactgcga ccaaagatcc gttccactgc
     3421 tgggctgaga taaatctctt catcgctgtt gggatttgtg atccaaaaac cttcttcaat
     3481 gttttcagcc atcaagcgga agcgttcttc tgacccgcgc agttgttctt cagcttttct
     3541 acgctcggta atatctgtgg ctacaaccag taccatgacc tcgccgctga catcgatcaa
     3601 gtccagcgat gcgaggcagt ctcgaa
//
LOCUS       DAOQNJ010000238         3617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17241_length_3617_cov_9.119596, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000238 DAOQNJ010000000
VERSION     DAOQNJ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17241_length_3617_cov_9.119596"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..824)
                     /locus_tag="PLG52_07820"
     CDS             complement(297..824)
                     /locus_tag="PLG52_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41392.1"
                     /translation="MSVLLIACGTPTVPPEVVSEAYPITEPALVLPDYKNATYLIEGS
                     FVTLADGIFEVEIAPGSTSKITTRYFGNEAIGDLNNDGKSDVAFLLTQDTGGSGTFFY
                     IVVALQTASGTEGSNAIFLGDRIAPQNTIIENGVITVNYADRNPGEPFTTPPSLGVSK
                     YFKIENNILTEVQNP"
     gene            complement(863..1654)
                     /locus_tag="PLG52_07825"
     CDS             complement(863..1654)
                     /locus_tag="PLG52_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNB41393.1"
                     /translation="MLPTSQKQTQFHTYHLDFLDDFNVPELDRSKWIPYYLPQWSSRA
                     KTQPRYRMEESCLILEIQPDQQPWSPEFNGSIRVSSLQTGLYSGPLHSAEGQHRFSKD
                     VRVREAQPTERTYTPQYGYFELKAKCNILPNNVAALWMIGFEEKPEHSAEICIFELKG
                     QNIREKSAIIGYGVHPFGDLSIRDEFYEDEFPVDATQFNTYAAEWTEREVHFYINDQK
                     IRTIQQSPQYPMQLMLNIYDLSDSPKQDRPMQFIVDYVSGYTLSK"
     gene            complement(1655..>1879)
                     /locus_tag="PLG52_07830"
     CDS             complement(1655..>1879)
                     /locus_tag="PLG52_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41394.1"
                     /translation="IMTFRRNSGIYFIKAGESAVTPGLGFTLLSLVLGWWGIPWGPIY
                     TIGSIYTNFNGGKDVTQQIMASIARPTPQA"
     assembly_gap    1880..1979
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2142..2594)
                     /locus_tag="PLG52_07835"
     CDS             complement(2142..2594)
                     /locus_tag="PLG52_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41395.1"
                     /translation="MKPIQIEIHGVTSKSSEEICAALLDTERWSDFGGYSILPGIKHA
                     HFEIRTPEVLGSRIIVQNTDGSAHIEEIVEWDPAKKVILKFQAFTPPLKNIASHFIET
                     WEFERINTETHTKRSMSMYPNHLFGWLILLPISRLMKKAFEQNAKQFS"
     gene            complement(2595..3020)
                     /locus_tag="PLG52_07840"
     CDS             complement(2595..3020)
                     /locus_tag="PLG52_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNB41396.1"
                     /translation="MHIPQSLIDLVESGAHAHLVTINKDGTPHVTVVWIGIENDEIVC
                     AHLPRNQKVRNIERNGNVILSLEADTKSSYGLKEYAVVHGTARITEGGAPELLQKLAY
                     VYMGPGVKFPPMDNPPPGYITRINVTRIEGVGPWVSKGE"
     gene            complement(3049..>3617)
                     /locus_tag="PLG52_07845"
     CDS             complement(3049..>3617)
                     /locus_tag="PLG52_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB41397.1"
                     /translation="YLVGASGGGHAALMVAATHPNVWAGVSVWVPITDLSVWYEETKT
                     LDGQYRIFLEQALGGAPGTSSAIDLEYKKRSPLTYLSPSIHLNLDINAGIHDGHNGPV
                     PISHSLLAYSALVTAEDQLSAKDIKYFVEQEKVPQHLIDPSLSDPSYGEKRPIFRAQS
                     GTTRLTIFEGGHELVIDAVINWLTQSLK"
BASE COUNT          882 a          739 c          887 g         1009 t
ORIGIN      
        1 agaagaacga cctgatcggt ttgaagggac atcgttctgt gggcggtatg cgcgcttcgg
       61 tttacaatgc catgagcatt gaaggcgcga aagaacttgc caagttcatg gcagagttcc
      121 agaagaagaa cggttagagt tcgtacagat tggaattaaa agacctccga attttcggag
      181 gtctttttct attaagagtc atctcaaaaa tgtcatcctg agcaatagcg aaggatctct
      241 accattgtgt aagagaccct tcggtcgcga agaacgcttc ctcagggtga cagggactag
      301 ggattttgta cttctgtgag aatgttattt tctatcttga agtacttcga gacacctagt
      361 gaaggcggtg ttgtaaacgg ttcgccggga tttctatctg catagttgac ggtaatgacg
      421 ccattctcaa tgattgtatt ttgtggggca atgcggtccc cgaggaagat ggcgttcgag
      481 ccttccgtcc ctgaggcagt ttgcaaagca acgacgatat agaagaatgt tccacttccg
      541 ccagtatctt gcgtgagcag aaaggcgaca tcacttttgc catcgttatt gaggtcaccg
      601 atcgcttcat tgccgaaata acgagtggtg atcttgctcg tggacccggg cgcgatttcc
      661 acttcaaaga tgccatctgc aagcgttacg aatgaacctt cgattaagta ggtggcattt
      721 ttataatcag gcaacacgag ggcgggctct gtaattgggt aagcttcgga tacaacttca
      781 ggcggtacag ttggggttcc gcaagcgatt aacagcacgg acaagcataa ggttataaaa
      841 attttcttca cggagcgttc ctttattttg agagcgtata gccactgacg tagtccacga
      901 tgaattgcat gggacggtcc tgtttgggcg aatcagacag gtcgtagata ttgagcatca
      961 actgcatggg gtattgcggc gattgttgaa tggtacgaat cttctgatcg ttgatataga
     1021 aatgaacttc gcgttctgtc cactcggcgg cgtaggtatt gaattgggtc gcgtccacgg
     1081 ggaattcgtc ttcatagaat tcatcccgaa tcgataggtc accgaaagga tggacgccat
     1141 agccgatgat tgcacttttt tctcggatgt tctgtccctt gagttcgaag atgcagatct
     1201 ccgccgaatg ttcgggtttc tcttcaaaac cgatcatcca caacgcagcg acattgtttg
     1261 ggaggatgtt gcatttggct ttgagttcaa agtagccata ttgcggagta tacgtccgct
     1321 cggtgggttg ggcttcgcgc acgcgtacgt ctttcgagaa gcggtgctga ccttcggctg
     1381 aatgcaatgg acctgaatag agtcccgttt gcaatgaaga gacgcgaata cttccattga
     1441 attcaggtga ccagggctgt tggtctggtt gaatttcaag gatgaggcaa ctctcttcca
     1501 tgcgatagcg cggctgggtc tttgcgcgcg aactccactg cggcaggtag tagggaatcc
     1561 atttggagcg gtctaactct ggcacattga aatcatcgag aaagtccaag tgataagtgt
     1621 ggaattgagt ttgtttttga gaagttggca acatttatgc ttgtggtgtg gggcgagcaa
     1681 tggatgccat tatctgttgg gtcacatctt tgccgccgtt aaaatttgtg tatatagagc
     1741 cgatggtgta gatcggtccc caagggatgc cccaccagcc aagcaccaac gacagtaggg
     1801 taaatcccag ccctggggta accgcacttt cgccagcttt gatgaaataa attccagaat
     1861 ttctgcgaaa ggtcatgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     1981 ttccaaataa gaatagcatc agccagagca agatgataat gttcgatgtg actcgtaaaa
     2041 attccaattg attggttatg gcatgaagaa caaggtcgaa gagttgcgta aaggctatga
     2101 taattcccag tgttttcagt gaaattattc gtatcatgtg tttaggaaaa ttgcttggca
     2161 ttttgctcaa aggctttttt cattagcctt gaaataggca ataaaatcag ccaaccgaat
     2221 agatgatttg ggtacatgct catgcttcgt ttcgtatggg tttctgtatt gattctctcg
     2281 aattcccaag tctcaataaa atgtgaagcg atatttttca atgggggagt gaaggcttga
     2341 aatttaagaa tgactttttt cgcaggatcc cattcaacga tttcttcaat gtgcgcagaa
     2401 ccatctgtat tttgaacgat gattcttgaa ccaagtactt cgggggtacg aatttcgaaa
     2461 tgggcatgtt tgatgcccgg caagatcgaa taccctccaa agtcagacca tcgttcggtg
     2521 tctaaaagcg cagcgcagat ttcttctgat gattttgatg tgacaccatg aatctcaatt
     2581 tgaatgggtt tcatctattc tcctttgctc acccacggtc ccacaccttc aatgcgagtg
     2641 acgttgatcc ttgtgatata gccgggaggg ggattgtcca ttggggggaa tttcacgccg
     2701 ggacccatat acacatacgc gagcttttgc aacaactccg gcgcgcctcc ctctgtaatt
     2761 cgagcggttc cgtggaccac ggcatattcc tttaacccat agctgctctt ggtgtccgct
     2821 tccaatgaga ggatcacatt cccattccgc tcaatgttac gcactttctg attgcgtggc
     2881 aggtgagcac aaacgatctc atcattttcg atgccgatcc ataccacggt gacatgtggt
     2941 gtgccgtctt tgttgattgt taccagatgt gcatgggcac cagattcgac caagtctatg
     3001 agagattgag ggatatgcat ggggtggctc ctgagtatag aaaaatactt atttaagact
     3061 ttgtgttaac cagtttatca ctgcatctat tacgagttca tgcccacctt caaatatagt
     3121 gagacgagtt gttccagatt gtgctctaaa gatcgggcgc ttttctccat atgaggggtc
     3181 tgacaatgaa gggtctatta gatgttgcgg cactttttct tgctcaacaa aatattttat
     3241 gtctttagca gataactgat cctctgctgt aactagagcg ctgtatgcta gtagtgagtg
     3301 gctaatgggc actggtccat tatgtccatc gtgaattcct gcattaatgt ctaggtttaa
     3361 gtggattgat ggagataaat atgtgagagg agagcgtttc ttgtactcta aatctattgc
     3421 agatgacgtg ccgggggcgc ctccgagagc ctgctctaaa aatattctgt attgtccatc
     3481 taatgtttta gtttcttcat accatacgga taggtctgta atcggaaccc atactgaaac
     3541 acctgcccaa acattggggt gggtggcagc caccataagg gctgcatgcc cgcctcctga
     3601 tgctcctact aaataaa
//
LOCUS       DAOQNJ010000239         3602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17383_length_3602_cov_11.371864, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000239 DAOQNJ010000000
VERSION     DAOQNJ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17383_length_3602_cov_11.371864"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PLG52_07850"
     CDS             <1..716
                     /locus_tag="PLG52_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB41398.1"
                     /translation="KWGTGVDSIDAEACSRFGVKLGRTPNAFTTPVADTVLGYMLAFA
                     RRGPWMDKAMKNGEWKKIPGKTLSECTLGIIGIGNIGKAVTRRAKAFGMKVLGTDIID
                     IDHVFVSESGIEMTDLDTLLANSDFVSVNCDLNSTSHHLINANTLSKMKSTAVLINTA
                     RGPIVDEKALVAALSSGQVGGAALDVFEFEPLPLDSPLLKMDNVMLAPHNSNSSPTAW
                     ERIHWNTIKNLVEGLGIVS"
     gene            740..2302
                     /locus_tag="PLG52_07855"
     CDS             740..2302
                     /locus_tag="PLG52_07855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB41399.1"
                     /translation="MKQLRLVLPYVFLFLLTALVLDLANPFFDKLARDGGFFLYAGQQ
                     IVEGKLPYRDIWDSKGPGIFYINALGLLLGGGSRWGVWLVEFLCIFGMFWLLYNSLSK
                     RWGIGAALFGATLAGLGLRTVLGYGNYTEEYALLFNAAGIVLFLSMRDVERNYWKYIW
                     IGAFFGLSFTFRANNIGGLFAIFGAVFLYYLIKQHFSEALRIILAALAGFALPLIVWA
                     LYFAVMGSAGEMFYASILFNFSYSAAKDREWLDIFGGFGKYGMGWVGWFTLVAWFLAG
                     FRMLVNFVQRKVTVFDTFMLLWFPIEIILSNLSGRNFTHYYISWVLAVAVYSAFVFAE
                     FWQLTFRVPSLEGWSDGFSVAASAGLIVMLFAISPATWGRYGESIRVGGEYIDPVSAY
                     IRDNSKPGDKLLTWYPEMGVNFMSGRASPVKYVYYPLFLEGSLTKDIEDSYFADITSN
                     PPELILDCSHSVDAIPSLDAATREEQYSTPGVKKKMYIQPNMNDIFEFVEQNYTLENT
                     IETCLVFRLKKD"
     gene            2421..3176
                     /locus_tag="PLG52_07860"
     CDS             2421..3176
                     /locus_tag="PLG52_07860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB41400.1"
                     /translation="MTRTVLITGAGGGIGRACINHFSSHGWRVIGVDRSDFGDNFPKD
                     GRFIKADISQPDSVEQIFQQAKEFHPALDALVNNAAVQVAKPLVDTTVEEWDSVMASN
                     LRSVFLFVKLAHPLLKAGNGGAIVNVSSVHAVQTSVNIAAYAASKGGLLALTRAMAIE
                     FAPDNIRVNAILPGAVDTQMLRAGLGRGHVGAGDLQDRLDNLARKTVNGRVGKPEEIA
                     HAIYFLADNEQSSFMTGQSIIVDGGATARLSTE"
     gene            3179..3529
                     /locus_tag="PLG52_07865"
     CDS             3179..3529
                     /locus_tag="PLG52_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41401.1"
                     /translation="MKFLQPARYFLLLISAFLSVLSILTLTRLGERPGMVGWYAIYSI
                     FMLIDSVVVLVCYFRLKQQSQRIFQVSMLILLLNMVLTIFDQIGVVDVIFIVLNLFAL
                     ILLYMARKEIAPVK"
BASE COUNT          854 a          736 c          901 g         1111 t
ORIGIN      
        1 cgaaatgggg aacgggagtc gattcaatcg atgccgaggc ttgttcccgt tttggagtga
       61 agcttgggcg aacgccgaat gcttttacta ctcctgtagc agatacggta ttgggctata
      121 tgctcgcctt cgcccgccgc ggtccgtgga tggacaaagc catgaaaaac ggtgaatgga
      181 aaaagattcc cggcaagact ctcagcgaat gcactttagg tattattggc atcgggaata
      241 tcggcaaagc ggtgactcgt cgcgctaaag cctttgggat gaaagttttg ggaacagata
      301 ttatcgacat tgaccatgtc tttgtcagtg aatctggtat tgagatgaca gacctcgata
      361 ccttgctcgc gaattctgat tttgtctctg tcaattgcga tctcaactca acatcacatc
      421 atttgatcaa tgcgaatacg ctttcaaaaa tgaagtccac tgcggtgctg atcaatacgg
      481 cgcgcggtcc tatcgtggat gaaaaagcct tggtcgcggc actcagttca ggtcaggtcg
      541 gaggcgcggc gcttgacgtc tttgaattcg agccattgcc attggatagt cctttgctga
      601 agatggacaa cgtcatgctg gcgccgcata attccaattc gagccctaca gcatgggagc
      661 gcattcattg gaacactatt aaaaatttgg tcgaaggttt gggaattgtg tcctgaaagg
      721 gtatgcaagt gaggaaataa tgaaacagct tcgcctcgta ttgccttatg tatttttgtt
      781 cttgctgacg gcgttggtgc tagaccttgc caatccgttc tttgataaac ttgccagaga
      841 tgggggcttt ttcctttatg ctggtcaaca gatcgtggag ggcaaactgc cctatcgtga
      901 tatttgggat tcaaaaggac ccggcatttt ttatatcaat gctttggggt tgttattggg
      961 tggcggttca cgttggggtg tttggctagt tgagtttttg tgtatttttg gaatgttttg
     1021 gcttttatat aactcacttt ccaaacggtg gggcattggt gctgcattat ttggcgctac
     1081 cttggcaggg cttggcttgc gaaccgtgct cggttacgga aattacaccg aagaatatgc
     1141 gttgcttttc aatgcggcag ggattgtatt gttcctttca atgcgagacg tggaacgaaa
     1201 ttattggaaa tatatttgga ttggcgcttt ctttgggttg agttttacat ttcgtgccaa
     1261 taacattggc ggtttgttcg ccatttttgg tgcagtcttt ttgtattacc tgatcaaaca
     1321 acatttttct gaagcgctgc ggatcattct tgctgcattg gctggctttg ctttgccgct
     1381 cattgtatgg gcattgtatt ttgccgtgat gggtagtgct ggtgaaatgt tctacgcctc
     1441 cattcttttt aacttctcct actcggctgc taaagatcgc gaatggctgg atatcttcgg
     1501 cggttttgga aaatatggaa tgggttgggt gggttggttc accttagtgg cttggttctt
     1561 ggctggtttt cgtatgttgg tgaactttgt ccaacgtaag gtgacggtct ttgacacctt
     1621 tatgttgctt tggttcccca ttgaaattat cttgagcaat ctttctggca ggaacttcac
     1681 acactattac atcagttggg tattggcggt ggcagtgtac tccgcctttg tctttgctga
     1741 gttctggcag ttgacctttc gcgtgccatc tttggaaggt tggagtgacg ggtttagtgt
     1801 agctgcctct gcggggctaa tcgtgatgct atttgccata tctcctgcga cttggggaag
     1861 gtacggcgag tcgattcgtg tgggtggtga atacatagac cctgtctctg cttatattcg
     1921 tgataatagc aaaccaggag acaagttgtt gacatggtat cccgaaatgg gcgtgaactt
     1981 catgtcaggt cgggcttcgc cggtgaagta tgtgtattat ccgctctttt tggaagggtc
     2041 tctcaccaaa gatattgaag atagttactt cgcagacatc acaagcaacc cacctgaact
     2101 gattctcgat tgttcccact ctgtggatgc cattccctct ttggatgctg caacacgcga
     2161 ggaacaatac tctacaccgg gtgtgaagaa gaaaatgtat attcaaccta atatgaacga
     2221 tatctttgag ttcgttgagc agaattacac acttgaaaat acgatagaga cttgcctagt
     2281 tttcaggctt aaaaaagact aggtttgtaa aattactata gatcagtaaa agcaacagac
     2341 gcgaattggg aaattcgcgt ctgttgcttt tattcatttt gcttcaggca caaagctaga
     2401 tttcgacgta aaattaccct atgactagaa cagttctcat cactggcgca ggcggcggaa
     2461 ttggacgcgc ctgcattaat catttttcaa gccacggttg gcgggttatt ggcgtagatc
     2521 gctctgactt tggagataat tttccaaaag atgggcgttt tatcaaagca gatatttcac
     2581 aaccagactc tgttgaacag atttttcaac aagcaaaaga atttcaccct gctttggatg
     2641 cattggtcaa taacgctgct gtgcaagtag ccaagccatt agtagatacc accgtagaag
     2701 aatgggattc ggtgatggca tcgaatttgc gttcagtatt tttgttcgtt aagctcgctc
     2761 atcctttgct caaagcaggg aatggcggcg ccattgtaaa tgtctcttcc gttcatgcgg
     2821 tacagacttc tgtcaatatt gccgcgtatg ccgcctccaa aggtggtctc ctcgccctta
     2881 cacgcgccat ggcaattgaa ttcgcgcccg ataatattcg cgtcaatgct attttgcccg
     2941 gcgctgtaga tactcaaatg ctgagagccg gtctcgggcg tggacacgta ggtgcaggcg
     3001 acctgcaaga ccggttggat aatctcgccc gcaaaacggt caatgggcgt gtgggaaagc
     3061 ccgaagagat tgctcatgcc atttacttcc ttgctgataa tgaacaatct tcatttatga
     3121 caggtcaatc cattattgtg gatggtggcg caacggcgcg tctcagcacg gagtaaaaat
     3181 gaaatttcta caacctgcgc gttattttct cttactgata tctgccttct tgtctgtact
     3241 gagtattctg accttaactc gtcttggcga aagaccagga atggtgggtt ggtatgcaat
     3301 atattcaatt tttatgctca tagattcagt agttgtgttg gtctgctatt ttcggttgaa
     3361 acagcaaagt caacgcatct ttcaagtgag tatgctcatt ctgcttttga atatggttct
     3421 gacaatattt gatcaaatcg gggtcgttga tgttattttt attgtcctga atttattcgc
     3481 gttgatcttg ctctatatgg cacgtaaaga aattgcacct gtaaaataaa tacaagaaaa
     3541 ggtctgtaga aaatgacacg cgcaagaaaa aagctgagca atcgatttca gtccagcctc
     3601 tt
//
LOCUS       DAOQNJ010000240         3579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17567_length_3579_cov_6.885358, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000240 DAOQNJ010000000
VERSION     DAOQNJ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17567_length_3579_cov_6.885358"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3098)
                     /locus_tag="PLG52_07870"
     CDS             complement(<1..3098)
                     /locus_tag="PLG52_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNB41402.1"
                     /translation="MDIPKLAQDAAQILAPLLPFLLQGAKDAARAAFERAGEKSFDLA
                     WKQGETLWEKLKGRVDTEQDAVKRALQKPEEKRVIQHLQSSLEFQLEDVFNADAELTK
                     SVNTVMAGDSSLAIGHDMKDSNATIGDGNFTASHGGQIHQTIQHQHFYGEPKKERVAR
                     KNKKVETEPLSDEETRIQYLNSIINDCKRARLVGLDPQAADVTRGAFSLDHLYISLDT
                     KTQVEIKKDEEADEKREFRFDKTRPLSVLEALSGKDEQSMVLLGLPGAGKSTFVRYLA
                     LWHAQALLDSKINFSEKLPKWNGTPMLPVIIPLGRLAESLPKESKRGTAEFIEKYFEQ
                     AMSFPTTLFKHTLEQGGLFLFDGLDEVADLGSRPIVVEAVEQFAAKYGKNRNSRFLVT
                     CRTFSYTDPRWQLTSWAAHELAPLTRKKINQFIEYWHNECIAVDPARKDDYEDKRKKL
                     HEALQEGDHRRLWEIADNPLILTVMAVVHTHKGDLPDARALVYEECIDLLLLRWEAER
                     TVTGKSQRKDLRSALNVPDIALRNVLQEIAYHAHERERKTDGKREGQAALVTEDLLRA
                     DLHAAFQNDEKVQIFMDYCESANGLLMLQGVAPLPDAPPDAPPRRVYAFPHLTFEEYL
                     AARYLRRMKTLGQKTFEHLTRSDRWREVVMLLGEHICFRDGDYELMDTILNALAPQPT
                     QPQTAEDWKARWAAGDLLLLYQRAFEQRKDQHTHIAHNLAALVQGSHLTSRERAEAAD
                     TLARLGDPRFDVDHWHLPKESLLGFVHIPAGEFLMGTREADIKGLIEKFGGDEGDYKS
                     ETPQHKLNLPDYFMARYPVTTAQFKAFVEESGHKPDMEASLRGLPTRPAVYVTWYDAI
                     AYCKWLSEKLKAVAPSRKGSNETEETFWRGLESGKFTVTLPSEAEWEKAARGGLPSPD
                     RRGAGGEVRNFPWGVDVNLDKVNGNMVIGRTSAVGAFPLGKSPYDLQDMSGNVWEWTR
                     SLWGKDYNLEYTYPYNKEDGRENLEAEKNVARVLRGGAYLYGGRYLRCAFRNRVDPDL
                     RV"
     gene            complement(3311..>3579)
                     /locus_tag="PLG52_07875"
     CDS             complement(3311..>3579)
                     /locus_tag="PLG52_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNB41403.1"
                     /translation="MQADTAVVPEIAEERYPFSAVKDANVLVFPSLESANIAYKLLAR
                     LGNAKAIGPILLGVGAPVHVLQTGDDVNAIVQIASVAVMDAMGR"
BASE COUNT          816 a          984 c          860 g          919 t
ORIGIN      
        1 ctgaccctga ggtcgggatc gaccctgttg cggaacgcac aacgcagata cctgcctcca
       61 tagaggtatg ccccgccacg cagaacacgc gcgacgtttt tctccgcttc cagattttcc
      121 ctgccatcct ccttattgta aggataggtg tattcaagat tgtaatcctt gccccacaaa
      181 ctgcgcgtcc attcccaaac attgccgctc atgtcctgca agtcataagg gcttttgcca
      241 agaggaaatg cgcccactgc gctggtgcgt ccgatgacca tgttgccatt cactttatcg
      301 agattaacat ccacgcccca cggaaagttc ctaacctcac ccccggcccc tctcctatca
      361 ggagagggga gtccgccacg cgcggctttt tcccattcgg cttcgctggg caatgttacc
      421 gtgaattttc cgctttcaag cccgcgccaa aaggtttctt cagtttcgtt tgaacctttc
      481 cggcttggcg caacggcttt gagtttttca ctcagccact tgcaataagc aatggcgtcg
      541 taccaggtga cataaaccgc cgggcgggtg ggtagtccgc gcaggctggc ttccatatct
      601 ggcttatgtc cgctctcctc cacaaaggct ttgaactgcg ccgtggtgac agggtaacgc
      661 gccatgaaat aatcgggcag attaagttta tgttgaggcg tttcgctttt gtaatcacct
      721 tcgtcaccgc caaatttttc gatcaagcct ttgatgtccg cttcgcgcgt gcccatcagg
      781 aactcaccag cagggatgtg aacgaaaccc aaaagggatt ccttgggtaa atgccaatgg
      841 tcaacatcga aacgcgggtc accgaggcgg gcgagggtgt ccgcggcttc ggcgcgttcg
      901 cgcgaggtga gatgggagcc ttggacgagg gcggcgaggt tatgggcaat atgagtatgt
      961 tgatccttcc tctgttcaaa agcgcgttgg taaagcaaaa gcaaatcacc agccgcccag
     1021 cgtgccttcc aatcttcggc ggtttggggc tgagtaggtt ggggagccaa agcgttcagg
     1081 atggtatcca tgagttcata gtcgccatcg cgaaagcaaa tatgttcgcc caacaacatc
     1141 actacttcgc gccaacgatc agaacgggta aggtgttcaa aggtcttttg ccctaaagtc
     1201 ttcatgcgcc gcaaatatcg ggcggcaagg tattcttcaa acgttaaatg tgggaaggca
     1261 tatacacggc gaggcggggc gtccggcgga gcatccggca ggggtgcaac cccttgcagc
     1321 atgagcaatc catttgcact ttcgcaataa tccataaaga tttggacttt ttcatcattt
     1381 tgaaaagcgg cgtgcagatc tgctcgaagc aagtcttcgg ttacaagcgc ggcttgccct
     1441 tcccgtttgc catctgtttt ccgttcacgc tcgtgggcat gataggcaat ttcctggagc
     1501 acatttcgaa gtgcaatatc cggcacattg agggcactgc gcaggtcctt ccgttgcgat
     1561 ttacccgtga cggttcgttc cgcctcccaa cgcaaaagca acaggtcgat acattcttca
     1621 taaaccaacg cgcgcgcatc tggcaggtcg cctttgtggg tgtggacgac cgccatcacc
     1681 gtgagaatca gcggattatc ggcaatttcc cacagacggc ggtgatcgcc ctcctgcaag
     1741 gcttcgtgca atttctttcg tttgtcttcg taatcatcct tgcgggcagg gtcaacagca
     1801 atacattcat tgtgccaata ctcgatgaat tgattgatct tcttgcgcgt cagcggcgcg
     1861 agttcatgcg ctgcccaact ggtcaactgc cagcgcgggt cggtgtagga gaatgtgcgg
     1921 caagtaacga ggaaacgcga gtttctattc tttccatatt tggcagcgaa ttgttcaacc
     1981 gcttccacca caataggacg gcttccaagg tctgcaactt cgtccaaacc gtcaaatagg
     2041 aacaacccgc cttgttcgag agtgtgcttg aataatgtgg taggaaacga catggcttgc
     2101 tcgaaatatt tttcaataaa ttctgctgtc ccgcgcttgc tttccttggg caggctttct
     2161 gccagacgtc ccagcgggat gatgacaggc agcatcggcg tcccgttcca tttgggaagt
     2221 ttttctgaaa agttgatttt ggaatccaac aacgcttgcg cgtgccagag ggcaaggtaa
     2281 cgcacgaagg tggacttccc agcccctggc aacccaagaa gaaccatgct ttgttcatct
     2341 ttgcccgata atgcctccaa tacgctcaac gggcgagtct tatcgaaacg aaactcccgt
     2401 ttttcatctg cttcttcgtc tttcttaatt tcaacctgtg tttttgtatc aagcgaaata
     2461 tacaagtgat cgagcgaaaa tgccccgcgt gttacgtcag cagcttgcgg atcgagccca
     2521 accagccggg cacgtttgca atcattgatg atactgttga gatattgaat tcgagtttcc
     2581 tcgtcagaaa ggggctcagt ctcaactttt ttgtttttac gagccaccct ttccttcttt
     2641 ggttctccat aaaaatgttg atgttggatc gtttgatgaa tttgcccacc atggctggcg
     2701 gtgaagttac catcgcctat ggtcgcatta gaatctttca tgtcatgtcc aatggcaaga
     2761 cttgaatccc cggccataac agtattgacc gatttcgtta gttcagcatc cgcattaaac
     2821 acatcctcca attgaaattc aagactgctc tgcaagtgct gaatcacacg tttctcttcc
     2881 ggcttttgta gggcgcgttt caccgcatcc tgttccgtat ccaccctgcc ttttaatttc
     2941 tcccacaacg tttcgccctg cttccaggca aggtcaaacg atttctcccc tgcgcgttcg
     3001 aaggcggcgc gggcagcatc ttttgcgcct tgaagcaaaa atggcaaaag gggagccaaa
     3061 atctgggcgg catcttgagc taatttggga atatccatgt ctcgcctcgc tggttcggat
     3121 gttaatattt gaattttacc ccgttataaa gagattgctt cgtcgggaaa cccaccctcc
     3181 tcgcaatgac atcatcaaaa caaaaaagac cccaaaggct cacaccttca gggtctcact
     3241 taccaattac caattaccac tcactacttt ccactcgcca atctctaatt ctctaatctc
     3301 tgctcttccc ctaccgcccc atcgcatcca tcaccgccac cgaagcgatc tgcacaatcg
     3361 cgttgacatc atcaccggtt tgcagaacat gcaccggagc gcccacgccc aaaaggatgg
     3421 gtccgattgc cttggcgttg ccgagacggg caaggagttt ataagcaatg ttcgcggatt
     3481 caagcgacgg gaagaccaac acattagcat cttttactgc actgaacgga tagcgttctt
     3541 cagcgatctc aggcaccaca gccgtatcgg cttgcattt
//
LOCUS       DAOQNJ010000241         3577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17588_length_3577_cov_7.287621, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000241 DAOQNJ010000000
VERSION     DAOQNJ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17588_length_3577_cov_7.287621"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1348
                     /locus_tag="PLG52_07880"
     CDS             173..1348
                     /locus_tag="PLG52_07880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB41404.1"
                     /translation="MLILDVRTLYVGIAIINLYTSLLLFLYSRQQKTYPGFGFWLIGN
                     VLALCMYSLYILRGAIPDFFGIVLANSIGTLANVFHLEGMKKFVKGSSPWLPNFLLPI
                     FIFIAHFYFLYIFNNLLIRDFLFSTFTILVVGRMSWLLVFETKEDIKPISLFFAALLL
                     IFIAGTISRVVLWNITSTTNIFQNTEINSTSALFLMIFDISWSVCLILLNSQRMNSEI
                     SQLTGQLEQFASLDSLTGVYNRRKFLEIGAAELERSKRYGRNLSLLMFDLNNFKDVND
                     SYGHAAGDEVLKKVVEICRNSLRNQDSMGRFGGDEFVILMPETNIEAAMEAAVRLNAE
                     VQNSTFKWDWNIHLSLSYGVASATAQDSNIDQLMHRADILLYEMKKYKKNLSFISST"
     gene            1372..2295
                     /locus_tag="PLG52_07885"
     CDS             1372..2295
                     /locus_tag="PLG52_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB41405.1"
                     /translation="MNFLITGAAGFLGSSLANHLAREGHQVRGLDDLSTGDPKVLSPD
                     VHFTRGDVSDRPKLWTLLQDVDVVYHVAARVSVQESILYPRDYNTVNVGGTVALMEAM
                     RDVGVKRVVLASSGAVYGDMSDGSLKESDIPNPRSPYAVSKISAEHYVRTIGNLWNIE
                     TVSLRIFNAYGPGQHLPPSHPPVVPHYLRQALRGGTLVAHGDGSQTRDYVYVDDVISA
                     MVAASTAPNLNGSVINVGSGMETSIKELINVVLSVTNSKANVVYNMQTSGGVSRMKAD
                     LTLAKEKLRFAPSISLVDGLKLTLQRDTRFK"
     gene            2305..2877
                     /locus_tag="PLG52_07890"
     CDS             2305..2877
                     /locus_tag="PLG52_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNB41406.1"
                     /translation="MTLPRKFYNRPTLTVARELLGARLVRILDGVKLVGLIAETEAYF
                     GFDDLASHAKAGRTIRTDPMFGPPGHAYVYFTYGNHWMLNTVTEQEGFPAAVLIRAIQ
                     PIEGVDVMMKRRQGRDAFGPGKLTQALGITKSENYADLTESGSSLWIEAGVKVPEKSV
                     TIGARVGLNTTPEPWFSKPWRFLVKNWKVV"
     gene            2890..>3577
                     /locus_tag="PLG52_07895"
     CDS             2890..>3577
                     /locus_tag="PLG52_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNB41407.1"
                     /translation="MGLLEGKNALIFGLANDKSIAWGIIQAFLREGANIGISYAGEIM
                     EKRVKPLAEKINCQWVEECDVTKDDQIEAVVKKAEKHFGKIDILVHSIAYAGRDELSK
                     PYHQTSREGFRVAMDISVFSFVALTNAFLPILNPGASVLCLTYGSGAVKVAPHYNVMG
                     VAKAALESSTRYLAYDLGAQKIRVNAISAGAIRTLAAAGVGGFRDMYKHFADKSPMRE
                     NVTIEDVGDAA"
BASE COUNT          907 a          875 c          861 g          934 t
ORIGIN      
        1 aaaccgcgag tcgctgatgt tcttcaaacc aacgtaacct accgttttcc tatctagccc
       61 caacaataac acacacgcta taaggagtca atcaaaaaca ggcaccaaat tggtgatagg
      121 gtgtacttcg ccgtataata aaatcaggca aatttaacgg taaaccctaa ttatgcttat
      181 tctcgatgtc cgcacccttt atgttgggat tgcaattatc aatctctaca cgagcttgct
      241 tctatttttg tatagccgcc agcaaaaaac ctatcccggt tttggcttct ggctgatagg
      301 caatgtactt gcattgtgca tgtactcact ttacatccta agaggggcga tccctgactt
      361 tttcggtatt gtgcttgcca atagcattgg taccttagcc aatgtttttc acctggaagg
      421 gatgaagaaa tttgtcaaag gttcatcccc ttggctgcca aacttccttt tacctatttt
      481 catctttatc gcccattttt attttctcta tatcttcaat aatcttctca ttcgcgactt
      541 tcttttcagc acctttacca ttctggttgt cgggcgcatg tcttggctgc tcgtttttga
      601 aaccaaagaa gacataaaac ccatctctct tttctttgct gcattactcc taatcttcat
      661 tgcaggcaca atatccagag tcgtgctatg gaatatcacc tccacaacaa atatttttca
      721 aaatacagag atcaactcaa cttcggcatt gtttctcatg atatttgata ttagttggtc
      781 tgtttgtctg atcttgttaa acagtcagcg catgaacagt gaaattagcc agctcaccgg
      841 gcaactggaa caattcgcct ctctcgactc actgactgga gtttacaacc gacgcaaatt
      901 ccttgaaatt ggagcagcag aacttgagcg ttcaaaacgc tatggcagaa atttaagtct
      961 gctcatgttc gacctcaaca atttcaaaga tgtcaacgat tcgtatggtc atgccgcagg
     1021 agatgaagtg ttgaaaaagg tcgttgaaat ttgccgcaat agtttaagaa atcaagatag
     1081 tatggggcgc ttcggcggag atgaattcgt cattctcatg ccagaaacaa atattgaagc
     1141 ggcaatggaa gccgccgttc gcctcaatgc agaagtgcag aactcaacct tcaaatggga
     1201 ttggaacatc catctctcgc tcagttatgg ggtggcttca gcgacagcac aagactcaaa
     1261 cattgaccaa ctgatgcatc gagcagatat attgctatat gagatgaaga aatacaaaaa
     1321 aaatctctcc ttcatttcat cgacttaatt tccgatgcta taatcttatt tatgaatttt
     1381 cttattaccg gagccgcagg gttcctcggc tcttcgcttg caaaccatct tgcccgtgaa
     1441 gggcatcaag tgcgcgggtt ggatgatctt tccaccggcg accccaaagt gctttcaccc
     1501 gatgttcact tcacacgtgg agatgtgagt gaccgcccca aactgtggac acttcttcaa
     1561 gatgtggacg tggtttacca tgtcgcggct cgcgtctctg tccaagagtc gattctgtac
     1621 ccacgcgact acaacactgt aaacgttggc ggcacagtgg cgctcatgga agccatgcgc
     1681 gatgtcggcg tcaaacgagt tgtgttggct tcttcgggcg cagtgtatgg agatatgagc
     1741 gacggcagcc tgaaagaatc agatatcccc aacccacgct caccctatgc tgtttcgaaa
     1801 atctcagccg agcattacgt ccgcacaatc ggcaatttat ggaatattga aaccgtcagc
     1861 ttgcggatct tcaacgctta cggaccgggt caacacctgc cgccttctca cccacctgta
     1921 gttccccact acctgcggca agcgcttcgc ggcgggacgt tggtagcgca cggcgatggc
     1981 agccagaccc gcgattacgt ctatgttgat gacgtgatca gcgccatggt ggcagcatct
     2041 acagcaccaa acttgaacgg atcggtcatc aacgtcggct cgggcatgga gacgtccatc
     2101 aaggaactca tcaatgtggt cttgagtgtg acgaacagca aagccaatgt ggtttacaac
     2161 atgcaaactt ctggcggcgt ctcgcgcatg aaagcagatt tgaccctcgc aaaagaaaaa
     2221 ctgcgctttg ctccgtctat tagtttggta gatgggttga agttaacact tcaacgcgat
     2281 acacggttta agtaatccgc ttaaatgacc ctccctcgca aattttataa ccgccctacc
     2341 ttaaccgttg cccgcgaatt gctcggggca cgcttggttc ggattctgga tggggtcaaa
     2401 ttagtcggct tgattgctga gaccgaagcc tattttgggt ttgatgacct tgccagccac
     2461 gccaaagccg ggcggacaat tcgcacggac ccgatgtttg gtccgccagg gcatgcctat
     2521 gtgtacttca cctacggcaa tcattggatg ttgaataccg ttaccgagca ggaaggtttt
     2581 ccggcggcgg tactgatccg cgcgattcag ccgattgaag gcgtggacgt gatgatgaag
     2641 cgccgtcagg ggcgggatgc gttcggaccc gggaaactga cccaagcgct gggaatcacc
     2701 aagagcgaaa attatgccga tttaacggaa tcaggctcaa gtctatggat cgaggcgggg
     2761 gttaaagtcc ccgaaaaaag cgtgacgatt ggcgcacgtg tggggttaaa tactacgccg
     2821 gaaccttggt tcagtaagcc ctggcggttt ctggtaaaaa attggaaagt tgtttaacga
     2881 ggagagacta tgggattact tgaaggaaaa aatgcgctga tcttcggatt ggcaaacgat
     2941 aaatccattg cttggggtat cattcaagcc tttttgcgcg agggtgccaa catcggcatc
     3001 agctatgctg gtgaaatcat ggaaaaacgc gtgaagccgc tggctgaaaa aatcaattgc
     3061 cagtgggttg aagagtgtga tgtgaccaag gatgaccaaa tcgaagcagt ggtgaagaaa
     3121 gccgaaaagc attttggcaa gatcgacatt ctcgtccatt cgattgctta tgccgggcgc
     3181 gacgaattga gcaaacccta tcaccagacc agccgcgaag gctttcgtgt ggcgatggat
     3241 atttcggtct tctctttcgt ggcgttgacc aatgccttcc tgcctatttt gaaccccggt
     3301 gcttcggtct tatgcctgac ctatggctcg ggtgcggtga aggtagcccc tcactacaat
     3361 gtgatgggcg tcgccaaagc agccttggaa tcctctacgc gctatttagc atatgacctc
     3421 ggtgcacaga agatccgcgt caatgccatt tcagcaggtg ctatccgcac attggcggca
     3481 gcgggtgtgg gcggcttccg cgatatgtac aagcactttg ccgataaatc tcccatgcgc
     3541 gagaatgtca ccattgaaga tgttggcgat gccgcgg
//
LOCUS       DAOQNJ010000242         3572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17629_length_3572_cov_7.189650, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000242 DAOQNJ010000000
VERSION     DAOQNJ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17629_length_3572_cov_7.189650"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1626
                     /locus_tag="PLG52_07900"
     CDS             <1..1626
                     /locus_tag="PLG52_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB41408.1"
                     /translation="KVFYLLTLLALVLSACTGGQPNQPAIARVGWAGSPDTLNPGMAI
                     LTEAYTMFELVYDSMYDLNLDGSFKLSLAESATVSDDGIVWTFKIRDGIKWHDGQPLT
                     AEDIAWTYNLYKDTPEYPYLNGYYTTYFDTIEATDNNEVVLTLTEAIPNIESQLVFLY
                     ILPKHIWENVDKLEYTNTEMIGSGPFKMTEYVQNEFVHLTANKDYFGGAPKIDEVIFQ
                     TFENQDALVQAIKTGQVDMITEMPNTAAESLKSEKDIELVVGAPLAPGVTDIIFNQVD
                     PENCPAEGGLCTGHPALRDRNVRLALAHATDKQNLIDVILLGLGKPGLTLIPDGLGVW
                     YNDSITDYEFDVAKANQILDDAGYIDADGDGIRDMPDGSRPLTFRLNWPSDSIPAPRM
                     AELLAEMWGKVGISLETQAVDPDALTAQCCPAFDFDIMIWGWASDPDPSSLLYVYTSE
                     GIPTGSSETGYSNPEYDALYAKQQVELNFDARKQLVWDMQKIVHDDVVYIIPFYDANV
                     QAYRTDRFTGWITDQAKVELSDVTSLTVIEPVK"
     gene            1699..2727
                     /locus_tag="PLG52_07905"
     CDS             1699..2727
                     /locus_tag="PLG52_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41409.1"
                     /translation="MDRTRAILRKAAWSVFTIFFVIILNFFLFRILPGDPARAGIRDP
                     RLNKDAVEALRVRFGLDKPIINCFESLNPIKIGSCSVNPLDTQFFIYVKNLLQGEMGF
                     SYHTNRPVVEVLGERLWNTILLIGVGQILAILFGVAFGIFAAWRARTSIDYASVGASL
                     VAWSLPTFWLAIVLLFWGSMIGLPVAGKATPGMSSYPLLQQWGDIAKHMLLPSITYTI
                     VYMGEYTLIMRSSLLDVLSEDYILTAKAKGLSTVQILRDHALKNAMLPLVTVIAINLG
                     FTVAGVIQIESVFSWPGLGSAIFEAVVRRDFPMLQGAFLLIAVAVIIANLLADLTYNY
                     LDPRVRVE"
     gene            2732..>3572
                     /locus_tag="PLG52_07910"
     CDS             2732..>3572
                     /locus_tag="PLG52_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41410.1"
                     /translation="MQITTSQTPAVPSRPASGLLSFWRSFSRSRTGLLGFWMLVIAIF
                     VAVFAPVIAPYERGASTSVQIADIYQPPSAAHWFGTDDAGQDVLTNFIYGARVSLTVG
                     FFAAFISIVIGGLFGLVAGFFGGRWENFLMRFTDIMLVIPDLPLTVVIVALTKPSLWN
                     IIFVIGILGWTTTARVVRSQTLAVKSRKFVLRARAIGASKIHILVQHILPLVLPILVV
                     QAVLAISLAILNESTLSFIGLGDPSAPSWGQMLNFAFGRGAMSVGAWWALFAPGFGIV
                     WVVL"
BASE COUNT          795 a          900 c          864 g         1013 t
ORIGIN      
        1 aaagtatttt atttgctcac cttacttgcc ttggttcttt cggcttgtac tggcggtcaa
       61 ccaaatcaac ccgccattgc ccgtgtaggc tgggctggca gcccagatac gctcaacccc
      121 ggcatggcaa tcttgaccga ggcgtacacc atgttcgagt tggtctacga ttcaatgtat
      181 gaccttaatt tagacggctc gttcaaactt agcttggcag agagcgccac cgtatcagat
      241 gatggtatcg tatggacgtt caaaatccgc gatggaatca aatggcacga tggtcagcct
      301 ttgacagcag aagatattgc ctggacctat aatctctaca aagacacccc agaatatccc
      361 tacttgaacg ggtattacac cacctacttt gacaccatcg aagcgaccga taacaatgaa
      421 gtcgttctga ccctgactga agccattccg aacattgaga gccagttggt attcctgtat
      481 atccttccga agcatatttg ggagaatgtc gataaactgg aatacaccaa tacagaaatg
      541 atcggctcgg gtccgttcaa gatgacggag tatgtacaaa atgaatttgt gcatctaaca
      601 gccaacaaag actatttcgg tggcgctccg aaaattgacg aggtcatctt ccagaccttt
      661 gaaaatcaag atgcgttggt tcaggcgatc aaaaccggtc aagtggacat gatcactgaa
      721 atgccgaata ctgctgccga atcgttgaaa tctgaaaaag atatcgaatt ggttgtcggc
      781 gcgccgcttg ccccgggtgt aaccgatatt attttcaatc aagttgaccc tgaaaactgt
      841 cctgcagaag gtgggctttg cacgggtcac cctgccttgc gtgatcgcaa cgtccgcctt
      901 gcgctcgcac acgccacaga caagcagaat ctcattgacg tcatcttgtt ggggcttggt
      961 aaacccggtc tcacattgat tcctgatggt ttgggcgttt ggtacaacga ctctattaca
     1021 gactatgaat tcgatgttgc caaagcaaac cagatccttg atgacgctgg ttacatcgat
     1081 gccgatggag atgggattcg tgacatgcca gacggctcac gccccttgac cttccgcttg
     1141 aactggccca gcgacagtat ccccgcccca cgcatggcgg agcttctcgc cgaaatgtgg
     1201 ggcaaagttg gtattagcct cgaaacacaa gccgtggatc ccgatgcttt gactgcccag
     1261 tgctgccctg cattcgattt cgacattatg atctggggtt gggcatctga ccctgaccca
     1321 agctctttgt tatatgttta cacgtctgaa ggcattccga caggttccag cgaaacaggc
     1381 tactccaacc ctgaatatga tgccctctac gccaaacaac aagtggagtt gaactttgac
     1441 gcccggaaac agcttgtatg ggatatgcaa aagattgtcc atgacgatgt tgtgtacatc
     1501 attccattct acgacgccaa tgttcaagct taccgcacag accgtttcac cggctggatt
     1561 accgaccaag ccaaagtgga actctcggat gttacatctt tgaccgttat tgagcctgtg
     1621 aagtaaaaac tttgaaatga aagacgttgc ccaacaggtt gattgaacct gttgggcaac
     1681 gttcaagaga taaaaccctt ggatcgcact cgcgccattc tccgaaaagc agcttggtct
     1741 gtttttacaa tattctttgt cattattctg aatttttttc tatttcgcat cctgcccggt
     1801 gaccccgctc gagcgggaat ccgcgacccg cgtttgaaca aagatgcggt ggaagcgttg
     1861 cgcgttcgct ttgggttaga taaaccaatt atcaattgtt ttgagtcgct caaccccatt
     1921 aagatcggga gttgctcggt taatccgctg gatacacaat tcttcattta tgtaaaaaat
     1981 cttttacaag gtgaaatggg atttagctat cacacgaacc gtcccgttgt agaagtcctt
     2041 ggcgaacgat tgtggaacac gattttgtta attggagtag gacaaatcct tgccatcctt
     2101 tttggggtgg cattcggtat ttttgccgct tggcgcgcgc gcacttccat agattacgct
     2161 tccgtcggcg caagtttggt cgcgtggagt ttgccgacct tttggcttgc tatcgttttg
     2221 ctcttctggg gcagtatgat cggcttacct gttgcgggta aagccacacc cggtatgagt
     2281 tcgtatccgc tattgcaaca atggggcgat attgcaaagc atatgcttct gccttctatt
     2341 acatacacga tcgtatatat gggcgaatac accctgatta tgcggtctag cttgttagat
     2401 gtgctttctg aagattacat tctgaccgcc aaagccaaag gcttgagcac ggttcaaatt
     2461 ttgcgagacc atgccctcaa gaacgccatg cttccgctcg tcaccgtcat tgcgattaac
     2521 cttggcttta cggtggctgg cgttattcag atcgaatcgg ttttctcttg gcccggcttg
     2581 gggagtgcta tttttgaagc cgtagttcgc cgcgactttc cgatgctgca aggcgccttt
     2641 ctattgattg ctgttgccgt tattatcgcc aacttgctag cagacctaac ctataactat
     2701 cttgaccctc gagtgcgcgt ggagtaactc aatgcagatt acaacttctc aaactcctgc
     2761 ggttccttct cgacctgcat ctgggttgct ttccttttgg cgttccttca gccgcagccg
     2821 tacgggtcta ctcggctttt ggatgctggt cattgcaatc tttgtggcag tatttgctcc
     2881 tgtcattgcg ccatatgaac gtggcgcatc cacctctgtc cagatcgcag atatttatca
     2941 accgccgagt gccgctcact ggtttggcac agacgatgcc gggcaagatg tgctcacgaa
     3001 ctttatctac ggcgcgcgcg tctcattgac cgtaggtttc tttgccgctt tcatttccat
     3061 tgtcattggc ggcttatttg gtttggtcgc tggtttcttc ggcgggcgtt gggaaaactt
     3121 cctgatgcgc tttaccgaca tcatgctcgt catcccagac ctgccattga cggttgtgat
     3181 cgtagcgttg accaagcctt ctctctggaa cattattttt gtcatcggca ttcttggctg
     3241 gacgacgacc gcacgtgtag tccgctcgca gaccttggcg gtgaaatcgc gtaaatttgt
     3301 tttacgtgct cgtgccattg gcgcaagtaa aatccatatt ctggttcaac atattctgcc
     3361 gttggttctg ccgattttgg tggttcaggc agttcttgca atttcgcttg ccatcttgaa
     3421 cgaaagcacg ctgtccttca tcggtttggg cgacccctct gcaccttctt gggggcaaat
     3481 gctcaacttc gccttcgggc gcggagcgat gtctgtcggt gcatggtggg cgttgtttgc
     3541 tccgggcttt ggcattgtat gggttgtcct ct
//
LOCUS       DAOQNJ010000243         3564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17695_length_3564_cov_6.903391, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000243 DAOQNJ010000000
VERSION     DAOQNJ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17695_length_3564_cov_6.903391"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLG52_07915"
     CDS             complement(<1..598)
                     /locus_tag="PLG52_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41411.1"
                     /translation="MSPNFSWIPIYQELAERLAGWQDQQSELIAFLETLRAEGYVITP
                     LNDKNTDGARFLLNEIDPFTFFGVFNRGIRQDQRKAILAQMKEFFALSHDVPEDFNGV
                     PILNNLKSWFFTNQTARQDGDVAKLWRVFQLGLGEKPLTNKEFLKSFDEALSVKQTNV
                     NLTMGLFWIRPNAFLSLDQNNRNHLEIKLPAEGLNAKFY"
     gene            complement(751..2016)
                     /locus_tag="PLG52_07920"
     CDS             complement(751..2016)
                     /locus_tag="PLG52_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNB41412.1"
                     /translation="MQILNACIDAVEPGNAIRRFVQREKNLLFIDNKKYDLNEFGNVT
                     VLGLGKATEAMSLALLDVLGSYPSRGLLIPKQAFASPASEFTVSPGGHPVPSEASVRA
                     GNAAFNLASSLTERDLLICLISGGGSALMASPRHGLQLADLQTLTKLLLACGARVDEI
                     NTLRRHLDRVKGGGIAKSANRARIASLILSDVVGNPLEAIASGPTALDPSTKEDALRI
                     LKKYDLLGKVPAAILEVLENSPETPKPDDPLFDRVQNVLVGSNSLAAQAGLEMAKSLG
                     FHTHFLGDTWQGEARDVAKELCGHFNLNYERPFCLIVGGETTVTLHGHGKGGRNQELA
                     LAAAIEMNGLENSMLITLATDGEDGPTDAAGVVVTGETCQRGERLGVDAQAFLSQNDA
                     NSYFEQLGDLLKPGPTGTNVNDLTFLFGF"
     gene            2103..3080
                     /locus_tag="PLG52_07925"
     CDS             2103..3080
                     /locus_tag="PLG52_07925"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNB41413.1"
                     /translation="MRPKVFVSRKIRDAGLNLVLEFCDAEIWDEEMPPTRDVLLEKVR
                     GVDGYLSMLTDRVDAELLDTAGPQLKVVSNHAVGFDNVVVSDATARGIPVGNTPGILT
                     DATADIAFALLLAAARRIVESEKYIRDGKWKTWHPSMLLGMDLVGRTLGLVGFGRIGR
                     AVAKRAVGFGMRIAYYSPNSEPEFGAIKMNSLDALLQESDFVSVHTPLNESTRHLVNA
                     DFLSKMKPNAVFVNTSRGPVVDQVALYDALKAKKIFAAGLDVTDPEPLPLDSPLLTLD
                     NCVIVPHIGSASERTRDAMARLAAENLIAGLKGERLPHSINPEVYENRR"
     gene            3180..>3564
                     /locus_tag="PLG52_07930"
     CDS             3180..>3564
                     /locus_tag="PLG52_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNB41414.1"
                     /translation="MNRTKNARVCIIGAGPSGITAGKNLIQAGVTNFVIYEKNDQVGG
                     NWVFNDKTKHSSVYETTHLISSRRYSEYFDYPMPADYPDYPSHTQMLAYFQGYAKHFG
                     VMPYIQFNTEVHNAEELPDKTWRITL"
BASE COUNT          896 a          842 c          942 g          884 t
ORIGIN      
        1 cataaaactt tgcgttcagt ccctcagcag gaagtttgat ctctaaatga ttgcgattat
       61 tttgatcgag actcaaaaaa gcgttcggac gaatccaaaa gagtcccata gtgaggttga
      121 cattggtctg tttgacggaa agcgcctcat caaacgactt gagaaattcc ttgttagtca
      181 gtggcttttc ccccagccca agctgaaaga ctcgccacag ttttgccacg tcgccgtctt
      241 gccgtgccgt ttgattggtg aagaaccatg atttcaaatt attgagaatc ggcaccccat
      301 tgaaatcttc aggcacatcg tgagagagcg cgaaaaattc tttcatctgc gcgaggatag
      361 ctttacgttg atcctgccgg atgccgcggt tgaatacgcc aaagaacgtg aatgggtcga
      421 tctcattcaa cagaaagcgc gcgccgtcgg tgttcttgtc gttcaacggt gtgatgacat
      481 agccttccgc ccgcagggtt tcgaggaacg cgatcaactc gctttgctgg tcttgccagc
      541 ccgcaagtct ttcagccaac tcttgataaa tgggaatcca actaaagttt ggcgacattt
      601 tttaattata aacgcctcgg caatggtagg ggcgacctgt caaaataagg aaagataatg
      661 ttacataaaa aggagggtcg ccctttatct agggcaaccc tcctttttgg ttaaatacca
      721 gaaaagtatg gacgggttgc ccctactata ctaaaaccca aacaagaatg tgagatcatt
      781 gacattcgtg ccggttggac cgggtttgag caaatcacct aattgctcaa aataggaatt
      841 cgcatcgttc tgactcagga aggcttgagc atccactcca agcctctccc ctcgctgaca
      901 agtctcccct gtcaccacaa cacctgccgc gtcggtggga ccatcttcgc cgtcggtggc
      961 gagggtgatg agcatggagt tttcgagtcc gttcatttcg atggcggcgg cgagggcgag
     1021 ttcttgatta cgcccgcctt tgccatgtcc gtgcagggtg acggtcgttt ctccacctac
     1081 gataaggcaa aatggacgtt cgtaattgag gttgaaatgt ccacacagtt ctttggcaac
     1141 gtcgcgggct tcgccttgcc aggtgtcacc gaggaagtga gtgtgaaacc ccagcgactt
     1201 tgccatttct aacccggctt gggcagcgag tgagttgctg ccaactaaaa cattttgcac
     1261 tcgatcaaat aatgggtcat ccggctttgg cgtttcagga gaattttcta acacttccaa
     1321 aattgcggca gggactttcc ccagtaagtc atacttcttc aaaatcctca gcgcgtcttc
     1381 tttcgtggat gggtctagtg cggtgggacc tgaggctatc gcttcgagcg ggttgccaac
     1441 gacatcggag agaatcaggc tggcaatccg cgccctattc gcagacttgg cgataccgcc
     1501 gccttttaca cggtcaagat gacggcgcag ggtgttgatt tcgtccacgc gggcgccaca
     1561 agcgagcaat aatttcgtca gcgtttgaag atcggcgagt tgaagtccgt gtcgcgggga
     1621 agccattaac gctgagccac cgccggagat gaggcaaatg agcaagtcac gttcggtgag
     1681 ggaagaggcg aggttgaagg cagcgtttcc ggcgcggaca ctcgcttcgc ttgggacggg
     1741 atgtccgcca ggcgagacgg tgaattcgct ggcaggggaa gcaaaggctt gttttggaat
     1801 gagcaagccg cggctgggat aagagccgag tacatccaac aaggcaagtg acatggcttc
     1861 tgtcgctttg cccaagccaa gtacggtcac attgccaaac tcattcaaat catatttttt
     1921 attatcaatg aataataaat tcttttcgcg ctggacaaaa cgtcgaatgg cattgcctgg
     1981 ttcgacggca tcgatgcagg catttagaat ctgcatgata tcgtttctgc ggggatgagt
     2041 ttgcagcgtg acagtggaaa aattgcgcat gtttcgagta tgataacgca gaaggagatg
     2101 caatgcgacc caaggtattt gtttcgcgca agatccgtga tgctggtttg aatttggtgc
     2161 ttgagttttg cgatgccgag atatgggatg aagagatgcc acccacgcgc gatgtgttac
     2221 ttgaaaaagt gcgcggtgta gatggttact tgagcatgct caccgatcgc gtggatgcag
     2281 aattattgga tacggctgga ccgcagttga aagtggtcag caatcacgcg gtggggtttg
     2341 acaatgtcgt tgtatcagat gcaacggcga gaggaattcc ggttggaaac acgccgggta
     2401 ttttgaccga tgccactgct gatattgcat ttgctttgtt gctcgccgca gcacgacgaa
     2461 ttgtagagtc tgaaaaatat atccgcgatg ggaaatggaa gacatggcat ccatccatgt
     2521 tgttgggcat ggaccttgta ggaagaactt tggggttggt ggggtttggt cgcattggac
     2581 gggcggtggc aaagcgtgcg gttggttttg gaatgcgcat tgcatattac agcccaaact
     2641 cagaacctga atttggcgcg atcaaaatga acagcctcga tgcgctgtta caagaatctg
     2701 attttgtttc ggtacataca cccttgaacg agtcgacgcg ccatttggta aacgcggact
     2761 ttctctcaaa gatgaagccg aatgcggtct tcgtcaacac ctcacgcgga ccagtggtag
     2821 accaagtcgc tttgtatgat gcattgaagg caaagaaaat tttcgcagca ggtttggatg
     2881 tgaccgaccc cgaacctttg ccgttggatt cgcctttact aaccttggat aactgtgtga
     2941 tcgttccgca cattggcagc gccagcgagc ggacgcgcga tgcgatggca aggctcgccg
     3001 cagaaaatct catcgccggg ttgaaaggcg aacgtttgcc acactctatt aatcctgaag
     3061 tatacgaaaa tagacgatag accacggaca atggacgata gaaagttcca tggtcaatgg
     3121 tctatcatcc atcgtctaaa caaggtaaaa ctctctcact tcatttaatc tgaggctaca
     3181 tgaatcgcac aaagaacgca cgagtttgca ttatcggagc aggtccctct ggcattacgg
     3241 ctgggaaaaa tctcattcag gcaggtgtca ccaatttcgt catctatgaa aagaacgacc
     3301 aagtcggcgg gaattgggtc ttcaatgaca agaccaaaca ctcgtctgtg tacgaaacga
     3361 cgcacctcat cagctcgcgg cgctattcgg aatattttga ttaccccatg cccgcagact
     3421 atcccgatta cccgtcgcac acgcagatgc tggcatattt tcaggggtac gccaaacact
     3481 ttggtgtgat gccttatatt caattcaaca ccgaagtcca caacgctgaa gaattaccag
     3541 ataaaacttg gcgcatcaca cttg
//
LOCUS       DAOQNJ010000244         3561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17717_length_3561_cov_6.792641, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000244 DAOQNJ010000000
VERSION     DAOQNJ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17717_length_3561_cov_6.792641"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..375)
                     /locus_tag="PLG52_07935"
     CDS             complement(139..375)
                     /locus_tag="PLG52_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41415.1"
                     /translation="MAISIQSNSGTPQEMDFMTVTDNDGKVPEMEFEGMTTRIAHDGY
                     EVLVKSILPFPQNRMGEIKTEDYRVGLVVIKSEN"
     gene            complement(606..1088)
                     /locus_tag="PLG52_07940"
     CDS             complement(606..1088)
                     /locus_tag="PLG52_07940"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HNB41416.1"
                     /translation="MNYKRIPMDHDKKIALVAHDNKKRDLLEWAKFNRDLLAHHQIYA
                     TGTTGKILEKELNLKVTKLKSGPLGGDQQIGAKIVENKIDFLIFFWDPLEPQPHDPDV
                     KALLRMAVVWNIPIANNRASADFMISSPLMDNPYERLVPDYETYTSRKIDLGDKSGKV
                     "
     gene            complement(1103..2422)
                     /locus_tag="PLG52_07945"
     CDS             complement(1103..2422)
                     /locus_tag="PLG52_07945"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB41417.1"
                     /translation="MTQKNNMHERPVEILQRLIQFNTTNPPGHEAACVGYIRDLLNEA
                     GIESTLVAKDPDRPNLIARLPGEGKAPPLLLYGHVDVVTTESQRWQQAPFEGKLIDGY
                     VWGRGALDMKGGVAMMVAAFMRANAEGLRPPGDIVLAIVSDEEAGGDYGAKFLVEEHP
                     ELFKDIKHAIGEFGGFTLTLGGKRFYPIMISEKQVCWMKATVRGQGGHGSMPEKGGAT
                     AKLATFLRALDEKDLPVHVTPATKLMVDAMASALGGLQGFILGQLTNPALTDLVLKTL
                     GSRVRSFYPLFRNTASPTIIHSSDKVNVIPSEISVELDGRLLPGQKPEDMIRELKAIA
                     GDDVEFELIQYEPGPAEPDMTLFNTLADILKEADPEGIPIPLILSGVTDGRFFSQIGI
                     QTYGYLPMTLPEDFNFADVIHAADERVPAAAIEFGAQAIYKALSRFG"
     gene            2552..2992
                     /locus_tag="PLG52_07950"
     CDS             2552..2992
                     /locus_tag="PLG52_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB41418.1"
                     /translation="MSTATQLREQALDGFWETIPTLWNFVRSYIRATATSKFDITVEQ
                     FHVLRFVRKGMSISELAIAKNISRPAISQAVDVLVKKGLLTRVQSTKDRRYVTLALTE
                     EGNALLDTVYKETRTWMKGRMSKLTNEELESIAKAMQAFKKILP"
     gene            3014..3547
                     /locus_tag="PLG52_07955"
     CDS             3014..3547
                     /locus_tag="PLG52_07955"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB41419.1"
                     /translation="MNKDIKEPIEPILSKNEVRRSGETTFVAMRHRNYQLYFGGQLIS
                     NIGTWMQVIAQAWVVYQLGHSELTLGLVAFASAIPNLIVSPWGGVIVDRISRRKVLIM
                     TQSGAMVLSFILAWLTFTNIVQEWHVILLAALTGVITAFDAPARQAITPELVGKKDVA
                     NAIAMNSMMFNRACSMA"
BASE COUNT          906 a          908 c          813 g          934 t
ORIGIN      
        1 agcacaagca ctttcttgcc agcttgtgcg agggcaactg cggcggtcaa cccgcccgcg
       61 cccgaaccga tgatgattgc gtcgtatgat gtcatgactt cctccgtgga ttcgtcgggc
      121 gaatgctaat gccccgtctt agttttcact ctttattact accaacccta ctcgataatc
      181 ttctgtcttt atctccccca ttctattttg cggaaaaggc aaaatgctct ttacgagcac
      241 ttcatatcca tcgtgcgcaa tacgagttgt cattccctca aattccatct caggcacctt
      301 gccatcgtta tcagtgacgg tcataaaatc catttcttga ggcgttccag aattagactg
      361 aatagagatt gccactgaaa caggtccact agcggcgcac tcaatattga ggggacaacg
      421 ctggtctccg ggaacactta gcagtgtaat cttcaagtct gttccgttta tggtcgcaga
      481 ttgccctggg accaaggtga attcagaatc aaggtcagca acaataggcg caagaggagc
      541 cgttggtatc agtccgcacg cggttatgca aaagagaaga acgcccacaa gaacgattct
      601 catcctcata ccttcccgct cttatcccct aggtcaattt ttcgcgaggt gtaggtttca
      661 taatctggca ccaagcgctc gtatggattg tccattaatg gtgaagagat cataaaatct
      721 gccgaagcac gattgttcgc gatcgggata ttccacacca ccgccatgcg caggagcgct
      781 tttacatctg ggtcatgtgg ctgcggctcc aacggatccc aaaagaagat gaggaagtct
      841 atcttattct caacaatctt ggcgccaatt tgctggtcgc cgccgagcgg tccgcttttg
      901 agcttggtca ctttgagatt tagttctttc tccaaaatct tgccagtggt gcctgtagca
      961 tagatttggt gatgggctaa taaatcacga ttaaactttg cccattcgag caagtctcgt
     1021 tttttattat catgtgccac caatgcgatc tttttgtcat gatccattgg aatacgcttg
     1081 taattcattc tgtctcctta cattacccaa aacgacttaa cgctttatag atcgcctgtg
     1141 cgccaaactc aatcgcggcg gctggcacgc gttcatctgc ggcatgaatc acatctgcga
     1201 agttgaagtc ttctggcaaa gtcatgggca aatagccata ggtttgaata ccaatctgag
     1261 agaagaagcg accatcggtc acgccgctaa ggatcaacgg aatgggaatc ccttcagggt
     1321 cggcttcttt caagatatct gccaaggtgt tgaaaagcgt catatccggt tcggcaggac
     1381 ccggttcata ttgaatcagt tcgaactcta catcgtcacc ggctatggct ttcagttcac
     1441 gaatcatatc ttcaggcttt tgacccggta acaaacgtcc atccaattcc acagaaattt
     1501 cactcggaat tacattcacc ttatcactgc tatgaataat cgttgggctg gcagtattcc
     1561 taaacaatgg atagaaggaa cgaacccgag aaccaagggt tttgagcaca agatccgtca
     1621 gcgcagggtt ggtcagttga ccgaggataa aaccttgaag tccgcccaga gccgaggcca
     1681 tggcatctac catcaatttt gttgccgggg tcacatgtac aggcaagtct ttttcatcca
     1741 acgcccttaa gaacgttgcc agtttggcag ttgcgccgcc tttctctggc atcgaaccgt
     1801 gaccgccttg tccacgcacg gtggctttca tccaacagac ttgcttttca gagatcatga
     1861 tcgggtaaaa gcgcttgccg ccaagagtta aagtgaaacc gccaaattca ccgatggcgt
     1921 gcttgatatc tttaaataac tcaggatgtt cctccacaag aaactttgcg ccgtaatcgc
     1981 cacccgcctc ttcatcgctg acgattgcca gcacaatatc cccaggcggg cgcaagcctt
     2041 cagcattggc gcgcatgaaa gccgccacca tcattgccac gccgcccttc atatctaaag
     2101 cgccacgtcc ccacacatat ccatcgatga gcttgccctc aaatggagct tgctgccagc
     2161 gttgactctc ggtcgtcacc acatccacat gcccatacaa caacaaaggc ggagccttac
     2221 cctcaccggg caggcgtgcg atcaaattag gtcggtctgg gtctttggct accagcgtgg
     2281 attcaatgcc cgcctcattc aacaaatcac ggatgtagcc tacacaagcc gcctcgtgac
     2341 cgggcgggtt ggtcgtgttg aactggatca gccgttgaag aatttcaacg ggtcgttcat
     2401 gcatattatt tttttgcgtc attgaaagct ccaatctata gatggcttcc aaattatatg
     2461 cctaatcccc ttcttgttgt ttttcgtaaa caggcataaa atagtttcaa tacttaacta
     2521 ttaataaaat tacacaattt gaaaggtttc aatgagcact gccacccaac tccgtgaaca
     2581 agccttggac ggcttctggg aaactattcc cactctttgg aattttgtcc gttcgtacat
     2641 tcgagccacc gccaccagta aatttgatat tacggttgaa cagttccatg tactgcgttt
     2701 tgtgcgcaag ggcatgtcga ttagtgagct ggcaattgcg aaaaacatca gtcgtcccgc
     2761 cattagccag gctgtagatg ttttggtcaa aaaaggatta ttgacccgcg tgcaaagtac
     2821 aaaagatcgt cgctatgtga cccttgcgct gaccgaagaa ggcaatgcct tgttagatac
     2881 agtttacaaa gaaacgcgca catggatgaa aggtcgcatg agcaagttaa ccaacgaaga
     2941 attggagtcg attgccaagg cgatgcaagc cttcaaaaaa atattgccct aactttagga
     3001 gtttgacata agaatgaata aagatataaa agaaccgata gagcctattc tttcaaagaa
     3061 tgaagtgcgt agaagcgggg aaacaacctt tgttgccatg cggcatcgta attatcagtt
     3121 gtactttggc ggacagttaa tctccaacat cggcacgtgg atgcaggtca tcgcacaggc
     3181 ttgggtagtg tatcagctcg gtcactccga gttgacgttg gggttggtcg ccttcgcttc
     3241 tgccattcca aacttaatcg tctctccatg gggcggtgtg atcgtagacc gcatatcgcg
     3301 ccgtaaagta ttgataatga cccagtctgg tgcaatggtg ttgtctttca tcctcgcctg
     3361 gttgacattt accaacatcg tgcaggaatg gcatgtgatt ctgctggcag ccttgacggg
     3421 tgtgattacc gcctttgatg ccccggcacg tcaagccatc acaccggaac tcgttggaaa
     3481 gaaagacgtc gccaacgcca ttgcaatgaa ctcgatgatg ttcaacaggg cttgcagcat
     3541 ggcttgatcc tgattatctc c
//
LOCUS       DAOQNJ010000245         3549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17812_length_3549_cov_9.449914, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000245 DAOQNJ010000000
VERSION     DAOQNJ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17812_length_3549_cov_9.449914"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..728)
                     /locus_tag="PLG52_07960"
     CDS             complement(45..728)
                     /locus_tag="PLG52_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB41420.1"
                     /translation="MTKILVIDDEPSITNLVSAYLKPEGYEVFTAADGNAGLKAARAF
                     KPDLIILDLMLPGIDGIELLSRLRRESDVYVIMLTARTDETDKIVGLSVGADDYVTKP
                     FSPRELVARVKAALRRINTGSSPSAERSVLSFKRVKMDVGAHTVLVDENPIELTSLEF
                     ELLKALAENRGRVLSREQLLEKVWGAEYFGEIRVVDVHLGHVRQKLGDESLIATVRGV
                     GYRFEDEPL"
     gene            complement(808..1545)
                     /locus_tag="PLG52_07965"
     CDS             complement(808..1545)
                     /locus_tag="PLG52_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41421.1"
                     /translation="MMLASKSLALVVFVFIFGGIAFSSAMGWWVTESTKEPVTFTEGE
                     FAGLSDPADIRGSYTFGDIANSFDVTPEILAQAFGITEGDPSAFAVKDLETMYLDSGY
                     EIGTNSVRMFVAFYSGLPYDLTAEETVLPQVAADILLEQGNPTSDQIAYLQTHTVDLS
                     MPEPVSAEPVVETTPIPEESDYVVKGKTTFGDLISWGVQKETIESIIGATMPDPAMKV
                     KDYASANGLDFETIKTALQGEVDKVKP"
     gene            complement(1542..2396)
                     /locus_tag="PLG52_07970"
     CDS             complement(1542..2396)
                     /locus_tag="PLG52_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB41422.1"
                     /translation="MVTLFGKRIRLFRPRSIWIRRVVQWFFFVLIGLIVLNHSLVESG
                     GGIPFLASASLHGVCPFGGVETLYTYLASGLFVQKIHDSSLVLAAIVILMSILFGPVF
                     CGWVCPLGTVQEWVSKIGKKLFKRRFNHFVPVKLDNALRYMRYVVLIWVLYVTATSGS
                     LIFEAYDPYFALFNFWSTEVAWSGLAILGVTLVLSVFVERPWCKYACPYGAVLGLTNL
                     FRVFSIKRAESTCKADGACSIMCPMNIQVDTVKTVRNHQCISCLECTSEAICPVAKTV
                     VFTSGGVK"
     gene            2510..>3549
                     /locus_tag="PLG52_07975"
     CDS             2510..>3549
                     /locus_tag="PLG52_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNB41423.1"
                     /translation="MANIQTNWDVIIIGGGAAGFFAAIRCAEENLNQRVLILEKASQP
                     LGKVLISGGRRCNVTHACFEPAKLITYYPRGGNELRGAFSRFQPKDTVEWFEERGVRI
                     KTESDGRMFPVTDDSNTIANTLKFSAKQAGVRVELGASVLSVEKSVKGGFRLEVQREA
                     QKVFVQTKKLMFATGGDRKSQSLIQALGHTIAPQVPSLFTFNIKDKRIHDLAGISVED
                     VTLKMDGLTARGPLLITHWGMSGPAVLRLSAWGARILFDKNYASLLTVNWLGDYKLDS
                     ALEVLQRNKDWHENARKKVSAHSAFSQIPIRLWKQLVYFIGEKNWADVSKAELQKLAQ
                     ELTAGEYKIQGKG"
BASE COUNT          976 a          913 c          849 g          811 t
ORIGIN      
        1 aataattttg cgccgagatg ttcgcgaatg gttttcataa tttcctataa tggctcatct
       61 tcaaaacgat accccactcc acgcaccgta gcgatcagcg actcatcacc aagcttttga
      121 cgcacatgac caagatgcac atccactacg cggatctcgc caaagtattc agcaccccac
      181 actttttcaa gcaactgctc gcgcgataaa actcgtccgc gattttcggc gagggctttg
      241 agcaattcaa attcgaggga tgttaactcg atgggatttt catcgaccaa aaccgtatgc
      301 gcccccacat ccattttgac gcgtttgaag gagagcacgc ttctttctgc gctcgggctt
      361 gaccccgtat taatacgcct caacgccgcc ttgactctcg ccaccaactc ccgcggactg
      421 aacggcttgg tcacataatc atctgctccc accgataagc ccacgatctt atcggtctca
      481 tcggtgcgag ccgtgagcat aatgacgtac acatctgact cgcggcgcaa gcgcgagagc
      541 aactcaatgc cgtctatgcc agggagcatg aggtcgagga tgatgagatc tggcttgaag
      601 gcgcgggcgg ctttcagccc ggcgttgcca tcggctgcag tgaaaacttc atagccttca
      661 ggcttaagat aagcactgac gaggttggta atggaaggtt cgtcgtcgat tacgaggatt
      721 ttggtcatgg caagattcta cttctgaatt tcatcaatat gggcgacctt ccaagatatc
      781 catctcggtt gggtcgccct ttcaaattta cggctttacc ttatccacct cgccttgcaa
      841 ggcggttttt atggtttcaa aatccagtcc attagcagat gcatagtctt tgaccttcat
      901 tgccgggtct ggcatcgttg ccccaataat ggactcgatt gtttctttct gcacacccca
      961 agagatcaaa tcaccaaacg tagtcttacc tttgacaaca taatctgact cttctggaat
     1021 gggtgtagtt tctacaacag gctctgccga aacaggttca ggcatactca aatctactgt
     1081 atgggtttgc aggtatgcaa tttgatctga tgtagggttt ccttgttcga ggagaatatc
     1141 ggcagcaact tgcggcagaa ccgtttcttc ggcggtcaaa tcatagggta agccagaata
     1201 gaaagccaca aacatacgta ccgagttagt gccaatctca tagccgctat caaggtacat
     1261 cgtttcaagg tctttcacgg caaaagcaga cgggtcgcct tctgtgatgc caaaggcttg
     1321 ggcaaggatt tcaggagtca catcgaacga gttggcgata tcaccaaatg tatatgaacc
     1381 gcgaatatct gccgggtctg aaagccctgc gaactcgcct tcagtaaaag ttaccggctc
     1441 ttttgtagac tcggtcaccc accaacccat cgcagaagaa aatgcaattc caccgaagat
     1501 aaagacaaac accaccaaag ccaacgattt agaagccagc atcatttcac acctccactg
     1561 gtaaacacta cagtttttgc gaccgggcag atcgcttcag acgtacattc caagcaggaa
     1621 atgcactgat gattacgaac cgtcttaacc gtatccacct gaatattcat cgggcacatg
     1681 atcgaacagg cgccgtctgc cttacaagta gattcagctc gtttgataga gaacacgcga
     1741 aagagattgg taagtcccaa tacagcgcca tacggacagg catatttgca ccacgggcgc
     1801 tccacgaaaa cggagagcac gagagtcacg cccaaaatcg caagcccact ccaagcaact
     1861 tcggtgctcc aaaaattgaa gagcgcaaag tacgggtcgt aggcttcaaa aatcaagcta
     1921 ccactggtgg cagtgacata tagcacccag atcaatacaa cgtagcgcat atagcgcagc
     1981 gcattatcta gttttacagg gacgaaatga ttgaagcggc gtttgaacaa cttcttgccg
     2041 atcttgctca cccattcctg cactgtgccc aacggacaaa cccaaccgca gaaaaccggt
     2101 ccgaacagga tggacattag gatgacgatt gctgccaaaa ccagagagga gtcgtgaatc
     2161 ttctgaacaa agagtccgct ggcaagataa gtgtagagcg tttccacgcc gccgaaggga
     2221 caaactccat ggagtgaagc cgaagcaagg aaaggtatcc cgccaccact ttcgaccaac
     2281 gagtgattaa gcacaatcaa cccaatcaat acgaagaaga accattgcac cacacgtcga
     2341 atccagatcg agcgcgggcg aaaaagacga atacgttttc cgaacagtgt caccattgaa
     2401 aacctccaaa attaaactgt tcgtagtgta gttttcctct ttacagagtc ttcaaagtat
     2461 ttgtaaagat ttggtaaaga agaatccggc gtttataatg atgcccatca tggcgaatat
     2521 acaaaccaac tgggacgtta tcatcatcgg tggcggggcg gcagggttct tcgcagccat
     2581 ccgttgtgcc gaagagaatc tgaatcaacg cgtactaatt ctggaaaaag ccagtcaacc
     2641 gcttggcaaa gtactcatct ccggcggcag acgttgcaat gtcactcacg cttgcttcga
     2701 gcctgccaaa ctcatcacct actatccacg aggtggcaat gaactgcgcg gcgcgttctc
     2761 gcgttttcaa ccgaaagata cggtcgagtg gttcgaagag cgcggcgtgc gcatcaaaac
     2821 cgaatcagat gggcgcatgt ttccggtcac cgatgattca aacaccatcg ccaacacctt
     2881 gaagttttct gccaaacaag cgggcgtgcg ggtcgaattg ggcgcatccg ttttgagtgt
     2941 ggaaaaaagt gtgaagggcg ggttcaggct tgaggtccag agagaggcgc agaaggtttt
     3001 cgtccaaaca aaaaaattaa tgttcgccac gggcggtgat cgcaagtcac aaagtcttat
     3061 ccaagcattg gggcatacca tcgcgccgca ggtgccgtcg ctgttcacgt tcaacatcaa
     3121 agacaaacgc attcatgacc tcgcgggcat atcggttgaa gatgtaactt taaaaatgga
     3181 tgggctgact gcgcgcggtc cgctgttgat cacacattgg ggcatgagcg gacctgccgt
     3241 gctgcgactc tctgcctggg gggcgcgcat tttatttgac aagaactacg cttcgctctt
     3301 aactgtgaat tggctcggcg actacaaact tgattctgcg ctggaagttt tgcaacgcaa
     3361 caaagactgg cacgagaacg cacgcaagaa ggtctcggca cattcggcat tttcgcaaat
     3421 cccgattcga ctttggaagc agttggttta tttcatcggc gagaaaaact gggcagatgt
     3481 ttccaaagca gaactgcaaa aactggcgca agaactcacg gcaggcgaat ataaaattca
     3541 aggcaaggg
//
LOCUS       DAOQNJ010000246         3540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17892_length_3540_cov_11.420086, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000246 DAOQNJ010000000
VERSION     DAOQNJ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17892_length_3540_cov_11.420086"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLG52_07980"
     CDS             complement(<1..875)
                     /locus_tag="PLG52_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNB41424.1"
                     /translation="MALEKQPGAFSDTAWHALEAEQVMRDLEVHDEGLTTAEAEKRLA
                     HYGQNQLQEAPRPGFLAMLWDQLNNFVVILLIVASVISALLGDYVEAIAIMAIVVLNA
                     ILGIIQEQRAEQALAALKKLAAPEAQVMRDGSRKSVPAYNLVPGDIVFLEAGNFIPAD
                     LRLLEAVNLRVEEASLTGESLPVQKNASTLLDKNVPLGDRKNTAFMGTVVSYGRGRGV
                     VTSTGMNTQLGLIATMLQSVESEETPLQRRLDQLGKSLSIGSLILVFVVFIVALFNYT
                     EVSGLFSDPINYFKT"
     gene            1147..1587
                     /locus_tag="PLG52_07985"
     CDS             1147..1587
                     /locus_tag="PLG52_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB41425.1"
                     /translation="MPAIKIPKSRDDFKAYYALRYKVLREPWGHPKGTEKDDFEAISE
                     HFMAVAEKTGEILGVIKLFEKEAGVGQVSHLAVATEHQHKGVGKFLVEAVEAQAREHG
                     FKTLGAMVRVTTTAFFEKHGYHIAGLPVPHLGTTHLVWMEKPLA"
     gene            complement(1633..2502)
                     /locus_tag="PLG52_07990"
     CDS             complement(1633..2502)
                     /locus_tag="PLG52_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNB41426.1"
                     /translation="MEYWPVVVIILQLIFLEGILSIDNAAVIGALVSPLPNDEEVPWP
                     GKLTWLGKALHPILGFQRMAALRVGLLGAYVGRGAMLFFTSFLIHNSWIKLVGAAYLI
                     HLAFDNLDDMAMGGDENGEVSPIEARSFWATVLTVEIMDLVFSIDNVVAAVSLSDKLW
                     VVMLGVGIGILTMRYAAGIFSYAVEKEPILKQAAYILVLNIGVELILSQVWKIEIPDL
                     LRFGISVATIVLTLAYAHIPFLQRFRFVLLWFAQGIGIINKLVDWLFMPIKGIFSLIG
                     GLFSRPSNSPTAE"
     gene            2624..3232
                     /locus_tag="PLG52_07995"
     CDS             2624..3232
                     /locus_tag="PLG52_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41427.1"
                     /translation="MKNFKSPLTYFFLTVGLIALLAFFGPEEKSLGSNVRLVYIHGAW
                     VLTAEIAFIFAALAGLLGLLLRRDLFHTWSAALGRTGIIFWVTYLPLSLLAMQTNWNG
                     LFLAEPRFRLAMIFAVTGILLQLGLWMFSIPWLTSLANIFYIIVLRVIFAQAQNIMHP
                     PPSPIFNSGNYVIIGFFVSLNLLAWLAAYFLTRFLLNLKPQN"
     gene            3241..>3540
                     /locus_tag="PLG52_08000"
     CDS             3241..>3540
                     /locus_tag="PLG52_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41428.1"
                     /translation="MKLRFQLAIFLFVRTVLNSIHRMVYPFLPIFARGLGIDLSTMSL
                     LMTGRSLLGATSPFLGPIADWRGRKFGMLSGLGIFILGIGLVALFPTFWALVIALG"
BASE COUNT          992 a          877 c          746 g          925 t
ORIGIN      
        1 aaggttttga aataattgat ggggtcagaa aatagaccgc tgacttcggt gtaattgaaa
       61 agcgcaacaa taaaaacgac aaagacaaga atcaacgaac caatactgag tgattttcct
      121 aattggtcga gccggcgctg aagcggagtc tcttcggact ctacactttg caacatggtg
      181 gcgatgagac ccaattgggt attcataccc gtgctagtga ctacaccgcg cccacgtcca
      241 tatgaaacaa ccgttcccat aaacgctgtg ttcttgcgat ctcccaacgg gacgtttttg
      301 tcgagcaatg tagatgcatt cttttgtaca ggaagtgact cgcctgtaag tgaagcttct
      361 tccacacgta agttgaccgc ttccagcaag cgcaaatcag cagggataaa gttcccggct
      421 tcaagaaaaa caatatcacc aggcacaagg ttataggctg ggacagattt ccgcgagcca
      481 tcgcgcatca cttgcgcttc gggtgcggca agcttcttga gggcggcaag agcttgttcg
      541 gcacgttgct cttgaataat accgagaatt gcattcaaga ccacaattgc catgatggca
      601 attgcttcaa cataatcacc cagcaaagca gaaataactg aagcaacgat caatagaata
      661 acaacgaaat tgtttaactg atcccaaagc atagcaagaa agcctgggcg gggcgcttct
      721 tgtagctggt tctgaccgta atgcgcaagc cgcttttcag cttctgcggt ggtcagacct
      781 tcatcatgca cttccagatc acgcattacc tgttctgctt ctaaagcatg ccaggcggta
      841 tctgaaaacg caccgggttg tttttctaat gccatctatg gtctcctgat aaaagataaa
      901 aaagaccacc acgaatcaaa acatcgtggt ggtcttgcca acgcaatttg cgtatggatc
      961 accgatggcg tttaaccaat ggtctgacga tgatccatcc tgtggggaac aggcggctac
     1021 tccccaacca aaagagagtg tactccactg caaaagtcac tgtcaaggaa aaggggctac
     1081 ggttttgtat ggcattctca agagcagtat aatgaaatcg aacaacagtt catttgtgga
     1141 gggaacatgc ctgctatcaa aataccaaaa tcacgagatg acttcaaagc ttattatgcg
     1201 ttgcgctata aagtattacg cgaaccatgg gggcacccca aaggcacaga aaaagatgac
     1261 tttgaagcca ttagcgagca ttttatggca gtggctgaaa agaccgggga aattctgggt
     1321 gttatcaagc tattcgaaaa agaagctggg gtagggcaag tttcacactt ggcagtcgct
     1381 acagaacatc aacacaaagg ggttggaaaa ttccttgttg aagccgttga ggcgcaagct
     1441 cgtgaacatg ggttcaagac tcttggtgca atggtgcgtg tgaccactac agcttttttt
     1501 gaaaaacatg gctatcatat tgcagggttg cctgttccac atctagggac aacccatctg
     1561 gtttggatgg aaaaaccact ggcatgaatc aaaaaaggat gagcattttt gctcatcctt
     1621 ttttctgtaa aattattccg ccgttggcga attggatggg cgcgaaaaaa gacctccaat
     1681 caaactaaag atgcctttga tcggcataaa gagccaatca accagtttgt tgataattcc
     1741 aattccctga gcgaaccaca gcaaaacaaa gcggaagcgc tgtaagaaag ggatatgtgc
     1801 atacgccagt gtcaatacaa tggtcgcaac tgaaatccca aaccgcagca aatcagggat
     1861 ttcaattttc cacacctgcg aaaggatgag ttcaacacca atattcaaaa caaggatata
     1921 cgcagcctgc ttcagaatgg gctctttctc tacggcataa ctaaaaatac cagcggcata
     1981 ccgcatggtc aaaatgccaa tgcctacacc caacatcacc acccataatt tatcagaaag
     2041 cgaaaccgcc gctacaacat tatcaatgct aaagaccaag tccattattt caacggttaa
     2101 aactgtagcc cagaatgaac gcgcttcaat aggagaaacc tccccatttt catctccacc
     2161 cattgccatg tcgtcaagat tatcaaatgc caaatggatc aaataagcag cgcccaccag
     2221 cttaatccac gaattgtgga tgagaaagct agtgaagaaa agcatagctc cacgccccac
     2281 ataggcacct aataagccca cacgcaaggc tgccatacgc tgaaaaccga ggatgggatg
     2341 caatgccttc ccaagccatg tcaatttacc gggccaagga acttcttcat cattcggtaa
     2401 cggcgacacc aacgcaccga tcacagccgc attatcaatt gaaagaatcc cctctaaaaa
     2461 aattaactgc aaaataatta caacaacggg ccaatattcc atatcacaag gagcctttca
     2521 ctccataaac tcgcaagtat ggattctatc ccaaaccgca aagattttcc tctttctttt
     2581 gaaaattttt gtaacaaata aatagcaatg ttaaaatcca accatgaaaa acttcaaatc
     2641 ccctctcacc tatttctttt taaccgtagg cttgatcgcc cttctagcct tctttggtcc
     2701 cgaagaaaaa tcattaggat caaacgttcg cctcgtttac atacatggag cttgggtact
     2761 caccgctgaa atcgccttta ttttcgcggc tcttgccgga cttttaggct tgctcctgcg
     2821 acgcgacctt ttccacacct ggagcgcagc tcttggtcgc acaggcatta ttttttgggt
     2881 cacatatctg cctttgtcct tgctagccat gcagaccaac tggaacggtc tctttcttgc
     2941 tgagccgcgg tttcgtcttg ccatgatctt tgctgttaca ggtatcctac tccaattggg
     3001 tctgtggatg ttcagtattc cgtggctcac atcgctggcg aatatttttt atattattgt
     3061 tctgcgggtt atctttgcgc aagcccaaaa catcatgcac ccgcctccat ccccaatctt
     3121 taactctgga aattacgtca tcatcggctt tttcgtatct ttgaatctgc ttgcttggct
     3181 tgcagcttat ttcctcaccc gcttcttgct taacctcaaa ccgcagaact gagcattctt
     3241 gtgaagctac gctttcaact tgccatcttt ttattcgtcc gcactgtgtt aaattcaatt
     3301 cacagaatgg tctatccgtt cttgcctatc tttgctcgtg ggcttggcat agacctctcc
     3361 accatgtctc tgctcatgac cgggcgttcc cttcttggtg ctaccagccc atttctaggt
     3421 cccattgccg attggcgtgg gcgaaaattt ggcatgttgt ctggcttggg gatttttatt
     3481 ctaggcattg gtcttgtggc gctcttccct accttttggg cattggtcat cgcattaggg
//
LOCUS       DAOQNJ010000247         3540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_17895_length_3540_cov_7.476327, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000247 DAOQNJ010000000
VERSION     DAOQNJ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17895_length_3540_cov_7.476327"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PLG52_08005"
     CDS             complement(<1..574)
                     /locus_tag="PLG52_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNB41429.1"
                     /translation="MQLIEPKVTPPLDEGFRPAVLADKNFRRNAQPVGVRAVIGLERG
                     AGEMSRFETIVLPEGHPDFESNYQYIERIVKFLLWQRGGHTVYVGAPKQIGEHIRKVY
                     AADGAQKFDFHFMGKQVYEKEFYVVVCSADEVPASRETGKLLGRNLAGCRIGFDLGAS
                     DRKVSAVVDGEAIYSEEVIWEPRKNTDPSYH"
     gene            complement(578..1726)
                     /locus_tag="PLG52_08010"
     CDS             complement(578..1726)
                     /locus_tag="PLG52_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNB41430.1"
                     /translation="MQKATHQQTKQHNRDLVLRTIFANESISRAEVARVTNLTRTTVS
                     DVVNSLLDEGLVEEVGRGESIGGKTPILLSVVADSRYLIGLNLAQDKFIGAIVNLRGE
                     IKEMVEVPVHGEDGKNALDLVYQIIDQLMKKRIKPIVGIGVGAPGLVNTREGVVVNAV
                     NLEWQDLPLGQLLEKKYKIPVLILNDSQATAIGEFVYGGAHALDENLIVVNVKHGIGS
                     GILVNGRLFQGDGGGAGEIGHVVVQENGELCRCGKHGCLETISSARAVLRQLNLKSLD
                     EAKSAFDAGDEKTQWVIKNTAHYLGISLANLIGILNIQKIIITGDMTRFGAPWMDEVN
                     NSMRNAALARLSENTKLELGALDYRACILGASAFLLLDDYSLLFQQEN"
     gene            1923..2612
                     /locus_tag="PLG52_08015"
     CDS             1923..2612
                     /locus_tag="PLG52_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41431.1"
                     /translation="MELIPFNLLGTLGIIGATIYAIGDVLLLASKISLDEYPKLKPFA
                     KLLSDSEKMVALSPIRMMWGALLGVFATPLVLFGFWHAYQGLSGVNAWAALIAFFLFA
                     LASIIGTFVHGTFYYMGEYVHALNKVSTDSQAVIVEMIERHKKVLIITYAPLLVFIIF
                     ASILFSILVASGNTLFPVWMTAINPLTLTILWMLGKRILPQFVRDWTEGAGFNIAYMA
                     FFACTTITLWK"
     gene            2616..3278
                     /locus_tag="PLG52_08020"
     CDS             2616..3278
                     /locus_tag="PLG52_08020"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNB41432.1"
                     /translation="MKTSTSIQDYLKRIYELTESGQSASTNDLARELKVKPASVTGMI
                     QKLAAETPALVEYQKHQGVTLTVAGKRAALEVIRHHRLLETWLVQTLGYSWDEVHEEA
                     ERLEHVISEDFERRIAAALGHPLRDPHGELIPTEDLKMPLDESTPLSALRKDQSGTIL
                     CIKAADADLLRHLTELGLTPNTFIQVTEYSPYDHNLTIRVDKKTHVLGLSITSKIFIE
                     EG"
     gene            3339..>3540
                     /locus_tag="PLG52_08025"
     CDS             3339..>3540
                     /locus_tag="PLG52_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41433.1"
                     /translation="MNETIFRILAAVILFTGVGISIYFRSKADRTSGEKLSRNVDGTP
                     LMLVIRIFGLVLWLSPLVYLVNP"
BASE COUNT          949 a          914 c          752 g          925 t
ORIGIN      
        1 aatgataaga aggatcggtg ttcttgcgcg gctcccaaat cacttcttcg ctgtaaatcg
       61 cttcgccatc caccacggca ctcactttgc ggtcagatgc gccaaggtca aacccaatgc
      121 ggcacccagc caaatttcgc ccaagcagtt tcccggtctc gcgtgaggca ggcacttcat
      181 ccgcactaca aacgaccaca taaaattctt tttcatagac ttgcttgccc atgaagtgaa
      241 agtcaaattt ctgcgcacca tccgccgcat acaccttgcg aatgtgctca ccaatttgct
      301 tcggcgcgcc cacatacacg gtatgccctc cgcgctgcca aagtaaaaac ttcacgatgc
      361 gttcgatgta ttgataattg gattcaaaat cgggatgacc ttcgggcaag acgatagtct
      421 cgaagcgcga catttcgccc gcgcctcttt ccaagccgat cacggctcgc acaccaacag
      481 gctgggcatt tcgccgaaaa tttttgtctg ccaacactgc cggacgaaag ccttcatcca
      541 atggcggtgt gactttgggt tcgatcaatt gcatggacta gttttcctgt tgaaacaaaa
      601 gtgaataatc atcgagcagc aagaacgcgg aagcaccgag aatgcaagcg cgataatcga
      661 gcgcaccgag ttcaagtttc gtattttcag agagccgcgc caacgccgca ttgcgcatgg
      721 agttattgac ctcatccatc cacggagcgc caaagcgggt catatcgccg gtgatgatga
      781 tcttttgaat gttcaaaata ccgatcaaat tcgcgagaga gattcccaga taatgggcgg
      841 tgttcttgat gacccactgt gttttttcat cgcccgcatc aaacgcagat ttggcttcat
      901 caagcgattt caaattcaat tggcgaagta ctgcacgagc gctggaaatg gtttcaagac
      961 agccatgctt cccacaacgg cataattcac cattctcctg caccaccaca tgaccaattt
     1021 cgcccgcccc gcccccatcg ccctggaaca aacgaccgtt gactaaaata ccagagccaa
     1081 tgccatgttt cacattgact acaatcaagt tttcatccaa agcatgcgcg ccaccataga
     1141 caaactcacc aatagcggtt gcctggctgt catttaagat caaaacaggg atcttgtatt
     1201 tcttttccag caattgacca agtggcaaat cttgccattc caaattgacg gcattaacca
     1261 ccacaccttc gcgggtgttg accaagcccg gagcgcccac tccaattcca acgatgggct
     1321 tgatgcgttt cttcatcaat tgatcaataa tttgataaac gaggtcaagg gcattcttgc
     1381 catcttcccc gtgaacggga acttcgacca tttctttgat ttccccacgt aaatttacga
     1441 tcgcgccaat gaatttatcc tgtgcgagat tcaaaccaat cagataacgc gaatctgcta
     1501 caacactaag aagaatcggc gtcttgccac caatcgattc gcctctgccg acttcttcaa
     1561 ccaagccttc atccaacaaa ctgttgacta catctgaaac tgtggtgcgt gtgaggttgg
     1621 taacacgcgc cacttctgca cggctgatag attcgtttgc gaagatggtt cgcagaacga
     1681 gatcacgatt gtgttgcttg gtttgctgat gagtcgcttt ttgcatgatt tttgccaaaa
     1741 tttctacacg cagaagtatc ttggataaca aacgatcatt taattacttt gtaaattcac
     1801 tgtactaaca aagtcagtat acagatttcc gcagcaagag tcaaggctga atctattgac
     1861 attttttagg ggatattata aaataattta ggtaaggcta aattatttta taaacgaaaa
     1921 ttatggaact tattcccttt aacttacttg gtactctggg catcattggt gcaaccattt
     1981 acgccattgg cgatgtgctt ttacttgcct caaaaatctc cctcgacgaa tacccgaaac
     2041 tcaagccatt tgccaaacta ctctctgact cagaaaaaat ggtggcactc tcccccatcc
     2101 gcatgatgtg gggcgcatta cttggagtgt ttgcaactcc cctcgtacta tttgggtttt
     2161 ggcatgcata tcaaggctta agcggagtca acgcctgggc agcattaatt gcttttttcc
     2221 tttttgcact ggcatccatc atcggaacct ttgtacatgg aacgttttac tacatgggcg
     2281 agtatgtaca tgccttgaac aaggtctcca ctgactcgca agcagtaatc gtagaaatga
     2341 ttgaaagaca caaaaaagtt cttatcatca cgtatgcccc gttgttggtg ttcattattt
     2401 tcgcttctat cctattttcg attttggtcg cttctgggaa tactttattc ccagtatgga
     2461 tgacagcgat caacccactt accctcacga ttctttggat gctcggaaag cgcattctgc
     2521 cgcaattcgt ccgcgattgg acggagggtg ccggtttcaa catcgcatat atggcattct
     2581 ttgcctgcac cactatcact ctttggaaat aatcaatgaa aacatccaca tccatacaag
     2641 attatctcaa acgcatctac gaactgaccg aaagcgggca atcagccagc accaatgatc
     2701 tagcgcgtga gctaaaagtc aagccagcat ctgtcactgg tatgatccaa aaacttgcag
     2761 cagaaacgcc tgccttggtc gaataccaaa agcatcaggg cgtcactctc accgtggctg
     2821 gcaaacgtgc cgctctggaa gtgatccgcc atcatcgttt gctggaaacc tggctcgtac
     2881 aaacccttgg gtattcatgg gatgaagtcc atgaagaggc agaacgattg gagcatgtca
     2941 tttcagagga tttcgagcga cgcatcgctg ccgctttggg gcatccgctt cgtgacccgc
     3001 acggagagtt gatccccacc gaagatctta agatgccgct ggatgagtcc actccgcttt
     3061 ctgctttgcg aaaagaccaa tctggcacga tcttatgcat caaagccgca gacgcagacc
     3121 tgcttcggca cctgaccgag ctggggctta caccaaacac gttcattcag gtgacagaat
     3181 attctccgta cgaccacaac cttaccattc gggttgataa aaagacacat gtgttggggt
     3241 tgagtatcac tagcaagata ttcattgagg aaggttaatc gacaaagtta tcgaataaat
     3301 gaaaagtaag aaacagaaag aagaaaggct ttaacactat gaacgaaacc atttttagaa
     3361 tcttggcagc cgttatcctt tttacaggtg taggcatctc catatatttc cgtagcaagg
     3421 cagaccgtac ttcaggagaa aaactatctc gcaatgtaga cggcactccg cttatgctgg
     3481 tcattcgcat ctttgggttg gtgctctggc tcagtccgct cgtctactta gtcaaccctg
//
LOCUS       DAOQNJ010000248         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_18074_length_3520_cov_7.321789, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000248 DAOQNJ010000000
VERSION     DAOQNJ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18074_length_3520_cov_7.321789"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="PLG52_08030"
     CDS             complement(<1..1779)
                     /locus_tag="PLG52_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41434.1"
                     /translation="MSADKSNITRASELTAANLRSVAKTISAQQLSRLTLPEIEAVVQ
                     LVSKIMPAGNVPGMILSGLARLPGRRIPVQKLQQDVTALFSGVEQILDSAVYGAFFAG
                     PAAVLWGYQNLLKLAGKDPESAFPEGMWQFYVDYALREDTARHMNETHGFDTILAQHN
                     IKLNKVDRLTAWFMAAVACLHQYPALLENEWRERVAISLLEKTLRDAGIESQRAKKIH
                     REWEIKRPYRREADGAQYDYPSYRRKKFDEFLQSRLQTMPELVYAAWEAGFNTALEQG
                     LRAYQRQMSIVAYLDPGPYGETRVPFDLSDAKIGIIHKDSYFLLPVCDEIGNPLDVMT
                     VRAQIASLLNSAFVSPSQISSLAKIKRSALAGLRSKLNPVLVNDLDNLKFAPILISTD
                     THSRALPLSELRQTERGVGSHALTIFDTGESFVFDQSHIFFDGAWGTALSEIMTNEAL
                     SWARYLSLLPSPVAPEMRLYTSLTLQLSPADLDLVQQAPKVMPEVGAETDKIDLKACM
                     SLRKDFKQRNDRIELTINDLLVLYRAVHAATYVPSQLVTNEIQKLSTVKPDVAASLHQ
                     LVEEGSRTNPAILIPMDASLKTPRDRV"
     gene            1876..2691
                     /locus_tag="PLG52_08035"
     CDS             1876..2691
                     /locus_tag="PLG52_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNB41435.1"
                     /translation="MLHGYRLTPHGRIALNFNASSLDELTGRLSDGFYTTFSTLAQGT
                     KVLGLKAHFDRLYQPAKELGLHPSADEQTVRKEIVDLTKALLPQESRVRLILTKDTGD
                     VYLGIQPLIPPSKAVYENGVHVITAEMARHDPRIKGTDFITQSAEQRKLVKDDVYEIL
                     LTKNGKVLEGMTSNFYAIKGKTIITARNGILLGVTRHAVLKLARGEGMSIQYRSPLVG
                     EKFDEAFLTSSSRGVVPIVQIGGTSVGQGKVGAWTKRLSLAYQAYVEARSESI"
     gene            2786..>3520
                     /locus_tag="PLG52_08040"
     CDS             2786..>3520
                     /locus_tag="PLG52_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41436.1"
                     /translation="MQKKILSLLLFMWVLSACGSSASEPQVITASGDITAAVEQYRAL
                     LGENNGGDPGTKGSSGYREINWDSLPDEESAPNLYAPDFFNQPEAPRARGIVLNTAGD
                     GLMVSASAANANGTLPRFGNINPQYADIFKTFSDEKLFSPVGSNIVEATFFVPGTNTP
                     AAVRGFGGIYADVDVDYYGFEFFDAAGTSLGKYQIPLADNGLAFVGVAFDEPVIARVR
                     IQFGTGVIGPDDGAGDVDISVMDNFIF"
BASE COUNT          910 a          815 c          895 g          900 t
ORIGIN      
        1 cacccgatca cgcggggttt tcaggctggc atccataggg atgaggatag caggatttgt
       61 acggctgcct tcctccacca attgatgtaa ggatgcggca acatctggct tgacggtgga
      121 aagcttttga atttcattcg tgactaattg cgaagggaca tatgtcgccg cgtgaacggc
      181 gcgatataac acaagcaggt cgttgatcgt caattcgatg cggtcattcc gttgcttaaa
      241 gtctttgcgg agcgacatgc aggctttcag gtcaatctta tctgtttcgg cgccgacttc
      301 tggcataacc tttggtgctt gctgaacaag gtctaggtcg gcgggagaaa gctgtaaggt
      361 gagagaggtg tacaaacgca tttcaggtgc aacaggggag gggagcaaac tcaaatacct
      421 tgcccatgaa agcgcttcgt tggtcatgat ctcagaaagt gccgtgcccc aagccccgtc
      481 aaaaaaaatg tgagattggt caaaaacaaa agattcgcca gtgtcaaaaa ttgtcaacgc
      541 atgggaaccc acgccgcgct ccgtttgacg taactccgaa agtggcaatg cccgcgaatg
      601 tgtgtctgta gagatcagaa ttggcgcaaa ttttaaatta tccaggtcgt tcaccaaaac
      661 cgggttaagt tttgagcgta gccctgctaa tgccgagcgt ttgatcttcg ctagagatga
      721 aatctgagac ggggaaacaa atgcagagtt caataaagaa gcgatctgcg ctcgaaccgt
      781 cattacatcc aacgggttgc caatttcgtc gcagacgggc agcaagaaat aactatcttt
      841 atggataatc ccaatttttg catctgatag atcgaatggc acacgcgttt caccataagg
      901 tccggggtcg agatatgcca caatcgacat ttggcgttga tatgctcgca agccttgttc
      961 taacgccgta ttgaagccag cttcccatgc cgcatacacc aactctggca tggtttgcag
     1021 tcggctttgt agaaattcat cgaacttctt acgtcggtag gatggatagt catattgcgc
     1081 gccgtccgct tcacgacgat aaggacgttt tatttcccac tcacggtgga tctttttcgc
     1141 gcgttgtgac tctattccag catcgcgcaa cgttttttcg agcaaagaaa tggcaactcg
     1201 ttcacgccat tcattttcaa gcaatgccgg gtattgatgc aaacaagcaa ctgcagccat
     1261 gaaccaagcc gtcagccggt caactttatt gagtttgata ttatgttgtg caaggatcgt
     1321 atcaaagcca tgcgtctcgt tcatatgacg ggctgtatct tcacgcagag catagtctac
     1381 ataaaattgc cacatccctt cggggaacgc agactcaggg tctttgccgg ctaacttcaa
     1441 cagattttgg tacccccata aaacagcagc gggtccggca aagaacgcgc catacacagc
     1501 actatccaag atttgttcta caccgctaaa aagagcagtt acatcttgtt gtaatttttg
     1561 gacggggata cgtcgaccgg gcaaacgtgc cagcccgcta aggatcatac cgggcacatt
     1621 ccctgccggc ataatcttgg agaccaattg caccaccgcc tcgatctctg gtaatgtgag
     1681 gcgtgaaagc tgttgcgcag aaatggtctt tgcaaccgag cgcaaattag cagcagtgag
     1741 ctctgaagca cgtgtaatgt tggatttgtc tgcgctcatc tatgaaatta tatttcacct
     1801 tcatcttgat ggattttttt gtctcgtccc aatctgtttg caaatttgtt aggtagcgga
     1861 tatataattc agctcatgtt gcatggatat cgacttacac cacatgggcg tattgccctt
     1921 aatttcaacg cctcttctct cgacgagtta acgggtcggc tttcagatgg cttttacacc
     1981 acctttagca ccctcgcgca agggacgaag gtgcttggct tgaaagctca ctttgacagg
     2041 ttatatcaac ctgcgaaaga acttgggctg catccctcag cagacgaaca aaccgtccgc
     2101 aaagagatcg tggacttgac aaaagcgctc ttgccacaag agtcccgcgt tcggcttatc
     2161 ctcacaaaag acacaggtga cgtttatctt ggcattcaac cattgattcc gccttctaag
     2221 gcggtgtatg aaaatggtgt gcatgttatc acggcagaaa tggctcgcca tgatccacgt
     2281 ataaagggaa cggacttcat cacccaaagc gcagaacaac gcaaattggt aaaagatgac
     2341 gtctatgaaa ttttgctaac caaaaatgga aaagtcttgg aaggcatgac ttccaatttt
     2401 tacgcaatca aaggcaagac gatcatcaca gcccgaaacg gaatcttact gggggtgaca
     2461 cgccatgccg tgctaaagct tgcgcgaggg gaggggatgt ctattcagta tcggtctccg
     2521 cttgtgggtg aaaaatttga cgaagccttt cttacatcca gttcgcgagg cgtggtgccg
     2581 attgtgcaaa ttggtgggac gtcagtgggg caggggaagg tcggggcgtg gacaaaacgt
     2641 ctctctttag cgtatcaggc ttatgtcgaa gcgagaagcg aaagcatcta aagttagaac
     2701 ctgtatctca ctaaagtcgg tgggatagat agcgcgacat ttatataatc gcgctatcta
     2761 tttttattgg acaaaggagt tcgagatgca gaaaaagata ctgagtttgt tattgtttat
     2821 gtgggtgctc tccgcttgcg gaagcagcgc ctcggagccg caagtgatta ccgcatctgg
     2881 cgatattacc gcggctgtag agcaatatcg tgcattgcta ggtgaaaaca acggtggcga
     2941 ccccggcacg aagggatctt cgggctatcg cgaaattaat tgggacagcc tgccagatga
     3001 agaatcagcg cctaatcttt atgcgcctga ttttttcaat caacctgaag cgccgcgagc
     3061 acgtggcatt gtcttaaaca cagcagggga tgggctcatg gtgagtgcca gtgcggcaaa
     3121 tgcgaatgga acgttgccac gctttggaaa tatcaatccg caatacgccg acatcttcaa
     3181 gacattcagc gatgaaaaat tattttcgcc ggtcggcagc aacattgtag aagccacgtt
     3241 ttttgtgcct ggcaccaaca ctcccgccgc tgtgcgcgga tttggtggca tctacgccga
     3301 tgtggacgtg gattattatg gctttgaatt ttttgatgcc gccggaactt cgttaggaaa
     3361 gtatcaaatt ccactggcag acaacggctt agcatttgtt ggcgttgcgt tcgatgaacc
     3421 tgtgatcgca cgagtgcgca ttcaatttgg caccggtgtg atcggacccg atgatggggc
     3481 aggggatgta gatatatctg tgatggataa ctttattttc
//
LOCUS       DAOQNJ010000249         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_18168_length_3510_cov_12.712301, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000249 DAOQNJ010000000
VERSION     DAOQNJ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18168_length_3510_cov_12.712301"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLG52_08045"
     CDS             <1..386
                     /locus_tag="PLG52_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB41437.1"
                     /translation="MGNIILCIEDNPDNMMLVKRALESRGYVLLEAFTGNKGVEVAEQ
                     NNVDLILLDINLPDIDGYEVARRLRSSSKHNLAYTPIIAVTANALKGDAEKALAAGCD
                     VYMSKPINIRELWARVEAFVPAPDP"
     gene            complement(447..2339)
                     /locus_tag="PLG52_08050"
     CDS             complement(447..2339)
                     /locus_tag="PLG52_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNB41438.1"
                     /translation="MKQPTFKQRFQYWFDNYMSKGTPALLFGLFVLSAIIIFAAAALV
                     KLTNTAPNGESLLQIAWMSLLRTLDSGTMGGDTGSPFFLFMMLIVTFGGVFVVSALIG
                     ILNNGLEDKLEELRKGRSVVLENDHTLILGFTPQIFTIISELVAANESRKSGAVIVVL
                     ADDDKVDMEDAIKERIPDTKNTRIICRSGSPMDMTDLEIASPHTARSIIILADGDDPD
                     TRVIKSVLAITNNPNRRNEPYHIVTQIRDPQNMDVIKLLSEKDTVQPILTTDLIARVV
                     AQTSRQSGLSVVYTELMNFGGDEIYFKQEPGLSGKTYGDALLSFETSTVMGIRRADGT
                     IMMNPAMDTRIEPGDSIFAIAEDDDKIQLTSSRLTLEENLIQRSGNRSKPKPERGLIL
                     GWNRSGSIIIRELDNYVAKGSELLVVSDVDGLESQIQREVGKLKNQKLKVMEGDIRDR
                     ALLDELGVTEYDHVIVLAYSHLDVQDADAITLVTLLHLRDIAEKDETPFSIISEMLDL
                     RNRELAEAAKVDDFIVSEHLISLMMAQLSENAELKDVFTDLFDPEGSEVYLKPISEYV
                     TTGEAVTYSTVVEAAKRRGETAIGYRIMSEVHDAEKSYGVRTNPKKSEKVTFASEDKL
                     IVISEN"
     gene            2604..3245
                     /locus_tag="PLG52_08055"
     CDS             2604..3245
                     /locus_tag="PLG52_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB41439.1"
                     /translation="MFQFEGFDRRKQEDKVREEGRLPPGQSLTQKFPVLHYGPVPTFN
                     PAAWDFRVWGEVEEEKRWSWDEFNQLPRTKLHMDIHCVTRWSKFDTDWEGVSVKTLVE
                     QGFFKIKPTATHVMQHAEYGFTVNLPLEVVLQENFLLATHFQGEPISPDHGYPLRGVV
                     GFIPGQEELETPYFWKGAKWLRSLEFMPRDKRGFWEQAGYHNRADVWREERFG"
     gene            complement(3292..>3510)
                     /locus_tag="PLG52_08060"
     CDS             complement(3292..>3510)
                     /locus_tag="PLG52_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNB41440.1"
                     /translation="RAGLKAGDKPTNFPGLMAGGDLIIAIDGREVRTFDDLLAYLITN
                     KGPGDTVVLTVLRGTEKVDISIVLDKRP"
BASE COUNT          916 a          789 c          889 g          916 t
ORIGIN      
        1 ttatgggaaa catcatcctc tgcatagaag ataatccgga taatatgatg ttggttaaac
       61 gcgcactcga atcacgcggg tatgttcttc ttgaggcttt cacaggaaac aaaggcgtgg
      121 aagtagcaga gcaaaataat gtcgatctga ttttgttgga tattaactta ccagatattg
      181 atggctacga agtagcaagg cgcttacgct ccagcagcaa acataacctt gcgtacactc
      241 ctatcattgc cgtaacagcc aatgcgttga aaggggatgc tgaaaaagca ctcgccgcag
      301 gatgcgacgt atacatgtcc aaaccgatca atattcgcga actctgggca agggttgaag
      361 cctttgttcc ggcgcccgat ccgtaatttt tattcaacaa aaaacacccc gagcaaaaag
      421 ctcggggtgt ttttattatc cgtctattag ttttcagaga ttacgatcaa tttatcttct
      481 gaagcaaagg taaccttctc agatttcttc gggttggtgc gtacgccata agatttttca
      541 gcatcgtgga cttcgctcat gatgcgatag ccaatagcag tttcaccacg gcgtttggcg
      601 gcttctacaa ctgtagagta ggtaactgct tcacctgtag ttacatattc gctaataggc
      661 ttgagataaa cctcagatcc ttcagggtcg aaaaggtcgg tgaagacatc tttcagttca
      721 gcattctcag aaagctgagc catcatcaaa ctgatgagat gttcgctgac gatgaagtcg
      781 tccactttgg cggcttcggc aagttcgcgg ttgcgcaggt cgagcatttc gctgatgatg
      841 gagaagggtg tttcatcctt ctcggcaata tcgcgcaggt gcaagagagt gacgagagtg
      901 atcgcatctg catcttggac atcgagatgg ctatacgcta acacgatgac atggtcatat
      961 tcggtcacgc cgagttcatc aagcaaggca cggtcgcgaa tgtcaccttc cataaccttg
     1021 agtttctgat tcttgagttt gcctacttcg cgctgaattt ggctttcgag tccatctaca
     1081 tcagacacga ccaaaagttc agagcctttg gcaacatagt tatccaattc gcgaatgatg
     1141 atggaaccag aacgattcca gccgaggata agtccgcgct caggcttggg cttggagcgg
     1201 tttccagaac gctgaatcag gttttcttcc agagtgaggc gagatgacgt aagttgaatc
     1261 ttgtcatcat cctcagcaat cgcgaagatg gagtcaccgg gttcaatgcg ggtgtccatt
     1321 gcggggttca tcatgatggt gccatcggcg cggcgaatac ccatcactgt ggaagtttca
     1381 aacgacagaa gagcatcgcc gtaggttttt ccagagagac cgggttcctg cttgaaataa
     1441 atttcgtcgc cgccaaagtt catcaactcg gtgtagacca cagacaaacc gctttggcgg
     1501 gaagtttgcg ctacgacgcg ggcaatgagg tcggtggtga gaattggctg gacggtatct
     1561 ttttcagaga gcagtttgat cacatccata ttttgcgggt cacgaatttg ggtgacgata
     1621 tgatacggct cattgcgacg attggggttg ttggtgatcg ccaagacaga tttgataacg
     1681 cgggtatctg ggtcatcgcc atcggcaagg ataataatag agcgcgcggt atgcgggctg
     1741 gcgatctcaa gatcggtcat atccattggg ctgccagaac ggcaaatgat gcgagtgttc
     1801 ttggtatcag ggatgcgttc tttgatggca tcttccatat ccactttatc atcatctgcc
     1861 aagacgacaa taacggcacc gctcttgcgg ctttcatttg cggcaaccaa ttcggagatg
     1921 atggtaaaaa tttgcggagt gaaaccaaga atgagggtgt ggtcattctc aaggacgacg
     1981 gagcgcccct tgcgaagttc ttcgagttta tcttctaacc cattgttgag gataccgatc
     2041 agggcgctga ccacaaacac accgccaaac gtgacgatga gcatcatgaa caggaagaac
     2101 gggctgccgg tatcgccgcc catggtgccg gagtcgaggg tgcgcagtaa gctcatccag
     2161 gcgatctgga gcaggctttc accattgggg gctgtgtttg tcagtttgac aagagcggcg
     2221 gctgcaaaaa tgataatggc agaaagaaca aataagccaa ataataacgc gggtgtccct
     2281 ttggacatgt agttgtcgaa ccaatattga aatctctgtt tgaatgtggg ctgcttcata
     2341 aaactatctc cttgcgcctt tgaaaatttg gcttattata agagcaattg catctatgac
     2401 cagagggtat tttcattaca tttgatttac atattttgca gatggtctta aacgacagag
     2461 gtcactgaga atttaagaaa agcatttcgg gcaattcaaa gctctagctt cttatacaat
     2521 tattagtttt gcagggtaac atctggcgtt tctttcatgt ctattccatg ccagtaggca
     2581 gactttagta aagtgaggta accatgttcc aattcgaagg ttttgaccgc cgcaagcagg
     2641 aagataaagt ccgtgaagag gggcgtcttc cccccgggca atcattgacc cagaaatttc
     2701 ccgttttgca ctatggaccc gtccccacat tcaaccccgc cgcatgggac tttcgtgtat
     2761 ggggcgaagt ggaggaagaa aaacgctgga gttgggatga attcaatcaa cttccacgca
     2821 ccaaacttca tatggacatc cactgtgtga cccgttggag caagttcgat accgattggg
     2881 aaggggtttc agtgaaaact ctagtggagc aaggtttctt caagatcaaa ccgaccgcca
     2941 cacacgtgat gcaacatgct gaatatggct ttacggtcaa cttgcccttg gaagtagtat
     3001 tacaagaaaa cttcctgctc gccactcact ttcaaggcga gccgatctca cccgaccatg
     3061 gttatccatt aagaggcgtg gtggggttca tccccgggca agaagaattg gaaacgcctt
     3121 atttttggaa gggtgccaag tggctgcgtt ctttggaatt tatgccgcgc gataaacgtg
     3181 gattttggga gcaagccggg tatcacaacc gagccgatgt ctggcgcgaa gaacgttttg
     3241 gttaaataaa aaaaactccg aagtctttga ggcttcggag ttttttttat tttatggtcg
     3301 tttatctaac acgatagaaa tatctacttt ttccgttccg cgtagaacag tcaacactac
     3361 ggtatcgccg ggacctttgt tggtgatgag atacgccaac agatcatcga aggtgcgcac
     3421 ttctctacca tcaatggcaa tgatcaaatc gccacctgcc atcaaaccgg ggaagttcgt
     3481 gggcttatca ccagctttca acccggcacg
//
LOCUS       DAOQNJ010000250         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_18254_length_3499_cov_9.245354, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000250 DAOQNJ010000000
VERSION     DAOQNJ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18254_length_3499_cov_9.245354"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..937)
                     /locus_tag="PLG52_08065"
     CDS             complement(26..937)
                     /locus_tag="PLG52_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41441.1"
                     /translation="MKHAVSISIGSSKRNKAVEVNLLGETVRIERIGTDGDMEAAALK
                     YKELDGKVDAFGVGGADLEALVDGKVYPFYSVRPMVRYVEKTPLVDGGGLKNTLENKA
                     VAFLDAKIGDYINQHGGRKVLVPVGVDRWGLSKSFAESGYETVFGDMMFGLDIPIPVR
                     SLGALRTVAALLMPIAGRLPFEMLYPTGEKQEKHVPKFQKYYHWATVIAGDCHYVKRH
                     MPLDGMQGKVVVTNTTTPDDVEFFRKVGIKYLLTTTPVLEGRSFGTNMMEAALVAVSG
                     KNRPLSWDELGEMITKLGFEPQLQELN"
     gene            complement(981..>3499)
                     /locus_tag="PLG52_08070"
     CDS             complement(981..>3499)
                     /locus_tag="PLG52_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB41442.1"
                     /translation="QANYLTALHETTFGLLNRLELKPLLETILLRTAHLLETPHVGID
                     LASVDGVVLRQEMGIGIYEQWNGNYTEKGEGLTGKVWESGNTIVVDDYETWEGKIPLV
                     SGMGLRSVLGAPLKSGDKILGVLLVASDKPNYFTSEKVTLLERLAALASLAIDNARLY
                     ESAQKEIRERKLVEEDLRSSEERFRKVFNNSSVAIAIVTLEDGIFLEANQAFWELAGR
                     TPQEALGRSAVDIDLWSNPEERARFVKDLLEEGSLENVEVEFSGKSGQSKLALGYYEL
                     LDIKGQRCILCMFYDVSVQRQAQSDLKESERRTRAIISSIPDMIFEISKEGDFLDFMA
                     SAELVPIMEPHEFIGKNIKNMFPPRIGEQTMFALDRALVTGQVHAFEYGLPPGEETQF
                     FEARVAPVTSESAIIMVRDISQRKWVETEREKLINELEEKNSESEALREGMTILVETL
                     DETKAVSLILEQLEKVLPFDSASVQLLKGDTLEIVSTRHLEPKEMHLGTRYVVSEKEP
                     SYPLLLGKVPYVLVKDVRATFTDLFAVAPYTLIRTWLAVPLRIKRQIIGIITLDGYQP
                     NKFTQRHAELALTYASQVAVALDNARLFSELQVELSARKNLIEELEVKNAELERFTYT
                     VSHDLKSPVITIRGFLGFLEQDALSGNVTRLRGDIQRIADATDKMQTLLNELLELSRI
                     GRLVNPPNLVPFNEIVSEALELVLGRIQKNHVQVHTQDEMEAVYVDRQRMVEAVQNLI
                     DNAAKFVGENPKIEIGQRGRDGNMLLFFIKDNGIGIPHVHHERIFGLFNKLDAISEGT
                     GIGLALVRRIIEVHKGRIWVQSEPGQGSTFFFTLPAGPES"
BASE COUNT          920 a          869 c          767 g          943 t
ORIGIN      
        1 ggacaatggc attcattttc atacgttaat tcaactcctg caattgcggt tcaaagccaa
       61 gcttggtgat catttcgcca agttcgtccc aagaaagcgg acggttctta ccagaaacgg
      121 ctaccaatgc ggcttccatc atattcgttc cgaacgaacg cccttccaat acaggtgtgg
      181 tggtaaggag gtatttaatt ccaaccttgc ggaagaactc tacatcatct ggcgttgtgg
      241 tgttggtcac aacaactttg ccttgcatac catctaatgg catgtggcgt tttacgtagt
      301 gacaatctcc ggcaataacg gttgcccagt gatagtattt ttggaacttt ggaacgtgct
      361 tttcttgttt ttcacctgtt ggatataaca tctcaaacgg caaacgtccg gcaatcggca
      421 tcaacaatgc cgcaaccgtt cgcaaggcgc ctaatgaacg aacgggaatg ggaatatcca
      481 aaccgaacat catgtcgcca aagaccgttt catagccaga ctctgcaaaa gacttagata
      541 agccccaacg atccacaccc accggaacaa ggactttgcg tcctccgtgt tgattgatgt
      601 aatcaccaat cttggcatcg aggaaagcaa cggctttatt ttcaagcgtg ttcttgagcc
      661 cgccaccatc tacgagtgga gtcttttcca catagcgcac catcgggcgc actgaataga
      721 acgggtagac tttcccatcc acaagcgctt caaggtctgc gccacctacg ccaaaggcgt
      781 ctacttttcc atctaattct ttatatttga gagcggcggc ttccatgtcg ccatcggtgc
      841 ctatacgttc gatgcgtact gtttccccca gcaaatttac ttccacagcc ttatttcttt
      901 tggatgaccc aatactgata ctgactgcat gtttcatgaa gaaacctcca cgagatgaga
      961 tgcgccagtt atatcacgaa tcaggattcg ggtccggcgg gaagcgtgaa gaagaaagtg
     1021 gagccttgtc ccggctcact ctggacccag atcctgcctt tgtgaacctc aatgattctc
     1081 cgcaccagag ctaaaccaat tcccgtgcct tccgaaatag cgtctagttt attgaacaag
     1141 ccaaagatcc gttcatgatg aacgtgcgga attccgatgc cattatcttt aatgaagaag
     1201 agcagcatat tcccgtctct gccacgttga ccaatttcga tcttggggtt ttctccgaca
     1261 aatttggcgg cgttgtcgat taggttttga acggcttcca ccatgcgttg acggtcgaca
     1321 tagaccgctt ccatctcatc ttgagtatga acctgcacat gattcttttg aatacgacct
     1381 aaaaccagtt ccagtgcttc actgacaatt tcattaaacg gaaccaaatt gggcgggttc
     1441 accaagcgcc cgatgcgtga aagttccagc aattcattca aaagagtttg cattttgtcg
     1501 gtggcatctg caatgcgttg aatatccccg cgcaaccggg tgacatttcc actaagggca
     1561 tcttgttcca gaaagccgag aaagccgcga atggtaatga ccggagattt aaggtcatga
     1621 gagacagtgt aggtaaagcg ttccaactcg gcatttttca cttcaagttc ttcgatcaag
     1681 ttcttgcggg cagacaactc cacttgtaat tcactgaaca gacgggcatt gtctaaagcc
     1741 acggctactt gactggcata ggttaatgca agttcggcat ggcgttgagt aaatttgtta
     1801 ggctgatatc catccaaggt gatgatgccg ataatctggc gtttgatgcg caaaggaact
     1861 gcaagccagg tgcggattaa ggtatagggt gccactgcga acagatcagt gaaggtcgcg
     1921 cgtacgtcct tcacaagcac ataaggaacc ttgcccaata aaagcggata agacggttct
     1981 ttctcgctga ccacatagcg cgtgcccaaa tgcatctctt taggctcaag gtgcctcgta
     2041 ctcacgattt ctaaagtatc gccttttaga agttgcacag aagccgaatc aaatggaagt
     2101 accttttcaa gctgttcaag aatcaagctg acggctttgg tttcatccaa agtctcaacg
     2161 agaatggtca tgccttctcg taaagcttct gattcagagt tcttttcttc caattcgttg
     2221 atcaactttt cgcgttcggt ttccacccat ttacgttgac taatatcacg caccatgatg
     2281 atggcagact cagaagtgac aggcgctaca cgggcttcaa agaattgcgt ctcttcgccg
     2341 gggggcaacc cgtattcaaa ggcatggact tgtcccgtga caagcgcacg gtctagagca
     2401 aacatagtct gctcgccaat gcgaggcggg aacatatttt tgatattttt gccaatgaac
     2461 tcatgcggtt ccatgatagg aacaagttct gctgaagcca tgaaatccaa aaaatcaccc
     2521 tcttttgaga tttcaaaaat catgtctgga atcgatgaaa taatggcgcg tgtacggcgt
     2581 tcactttctt tcaaatcact ttgtgcttgc cgctgcacag agacatcata gaacatgcat
     2641 aagatacagc gctgtccctt aatatcgagc aattcataat agccaagtgc taatttactt
     2701 tgcccacttt ttccagaaaa ttccacttct acattttcaa gagagccttc ttctagcagg
     2761 tcttttacaa accgggcacg ttcttctgga tttgaccaga ggtctatatc gacagcggaa
     2821 cgtccgagag cttcttgagg ggtgcgccct gccaattccc agaatgcctg attcgcttcc
     2881 aagaagatgc catcttccaa tgtcacaatg gcaatcgcca cactactatt gttaaacact
     2941 ttacggaagc gttcttcgct agatcgtaga tcctcttcta caagtttgcg ttcacgaatc
     3001 tctttttggg cgctttcata cagacgggca ttatcaattg ccaaagaagc aagtgccgcc
     3061 agccgttcga gcaaggtcac tttttcagaa gtaaagtaat tcggtttatc agaagcgact
     3121 aagagtacac caagaatctt gtcacctgat tttaatgggg cacctaaaac agaacgcaac
     3181 cccattcctg ataccaatgg gattttccct tcccatgttt cataatcatc aacaacaatc
     3241 gtgtttccgc tttcccaaac tttccctgtc agtccttcgc ctttttcagt gtagttgcca
     3301 ttccactgtt catagatgcc gattcccatc tcttggcgca agacaacacc atctacagaa
     3361 gcaaggtcaa tgcctacatg gggagtttct aatagatgtg ccgtacgaag aagaatcgtt
     3421 tcaaggagag gcttgagctc aagacggttg agcaagccaa aggtcgtttc gtgaagcgct
     3481 gtgagatagt tagcttggg
//
LOCUS       DAOQNJ010000251         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_18367_length_3485_cov_8.754519, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000251 DAOQNJ010000000
VERSION     DAOQNJ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18367_length_3485_cov_8.754519"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLG52_08075"
     CDS             <1..188
                     /locus_tag="PLG52_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNB41443.1"
                     /translation="ASVSKVGEEQLFYLMSRGLSEEEATTMVVSGFIEPLVKELPMEY
                     AVEMNRLIQLQMEGSIG"
     gene            190..1527
                     /gene="sufD"
                     /locus_tag="PLG52_08080"
     CDS             190..1527
                     /gene="sufD"
                     /locus_tag="PLG52_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNB41444.1"
                     /translation="MQEKPKVTVSRGRRADTAAKEFTFTQADVPSANGLASFRAGAWD
                     LFSKQNLPDTSLEAWRRTDLRLLPAPDFKLPKAGAFEDLPAVPEELLKPLTGEQHGGQ
                     ITLLPGGSKVDLDEALAKKGVVFTDFRTAEKNHSDLLKKLIGQIVKPDDGKFAALASA
                     LSENGVLLYVPKGVQVEQPLHSVLWGPGTDLAHFSHLLVYVEDGASVTYVHEAASPDE
                     TGHAMHAGIVEIYVGKDANLRFVELQSWGRHVWNFSHERARVDRGGNLDWIFGAVGSR
                     LTKNFSDLDLIGEGAQGRMSGFYFTDGTQHLDHDTQQNHMAPHCTSDLLFKGALKGKS
                     RSVWQGMIYVAPGAQKTDGYQANRNLVLDDQARADSIPGLEILADDVRCTHGATVGKL
                     EQEPLFYLKSRGIPQKEAEQLVVEGFFDPIMQRIPFEGVRERFQQAIQIKLTA"
     gene            1662..2267
                     /locus_tag="PLG52_08085"
     CDS             1662..2267
                     /locus_tag="PLG52_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41445.1"
                     /translation="MAVKSKTKSNSKTKVVVKAKTATKKGTVKVSAARKVVSKSKTKV
                     VAKSVKPSKSVVSSIKKKPASAEKPVEVTRTINLSSQIRQPAKPVLRRSSVGLGATNS
                     NPFTFMKAPRPEYAFEVGDTVEVFCDHEKGRERVRGWIKGIVVQVDNKMVAVQFRSNV
                     FLTDGWMVPDRILWYPLNSEHIRPGTTGKKPALKKDIIPDY"
     gene            2345..>3485
                     /locus_tag="PLG52_08090"
     CDS             2345..>3485
                     /locus_tag="PLG52_08090"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNB41446.1"
                     /translation="MTLNINEIRKDFPILERETANGKRVIYLDSTATSQKPLQVIEAM
                     NDYYRRSNANIHRGVHTLAEEATSLYEGARERIAKFIHAATSREIIYTRNTTESINLV
                     AYSWARANLKAGDLVILTEMEHHSNLVPWHMLQAERGIELEFIPVTEDGLLDLEAYKT
                     LLNRTPKLVSFTHMSNVLGTINPAAEIIKLAHAAGAVTLVDGAQSVPHLAVDVQALDA
                     DFYAFSAHKMCGPTGMGVLYGKSALLEAMPPFLGGGDMIKEVKLRSFRPNTLPHKFEA
                     GTPAIAEAIGLGAAVDYLTQIGMSDIAAHEHEITEYALERLEEIPGVKLFGPSADKKG
                     GVAAFTLEGVHPHDVAQILDQDGIAVRAGHHCAQPLHEKFGIPATS"
BASE COUNT          872 a          860 c          897 g          856 t
ORIGIN      
        1 aagcctcggt cagcaaagtg ggcgaggaac agctcttcta tctcatgagc cgtggactca
       61 gcgaagaaga agccacaacc atggtggtct ctggcttcat cgaaccgctc gtcaaagaac
      121 tccccatgga atacgcagtc gaaatgaatc gactcatcca gttacaaatg gaaggatcga
      181 ttggctaaca tgcaagagaa accaaaagta accgtatctc gaggacgtcg cgccgacact
      241 gccgcaaagg aatttacctt tacgcaggca gatgtccctt ccgcgaacgg actcgcttct
      301 ttccgcgccg gcgcctggga tttattctcc aagcagaatc tccccgacac ttctctcgaa
      361 gcctggcgcc gcaccgattt gcgtttattg cccgcacctg attttaaact tcccaaagcg
      421 ggtgccttcg aagatttgcc agccgttcca gaagaattac tcaagccgtt gaccggtgaa
      481 cagcacggcg gacaaatcac tttattgccc ggtggctcta aagtggactt agatgaagcc
      541 ctcgctaaaa agggcgtcgt cttcactgac ttccgcacag cagaaaagaa tcactctgac
      601 ttactcaaga aattgatcgg tcagatcgtc aagccagatg atggcaagtt tgccgccttg
      661 gcttcggcac tttctgaaaa tggcgttctt ctttatgtgc ccaagggcgt acaagtggaa
      721 caaccccttc actctgtgtt gtggggaccc ggtactgacc tcgcgcattt ctcgcatcta
      781 ctcgtttacg tggaagatgg cgcttcagtg acgtatgttc acgaagccgc ttcacccgat
      841 gagaccggtc atgccatgca tgcgggaatt gtcgaaatct atgtagggaa agatgccaac
      901 ctgcgctttg tagaattaca gtcgtggggt aggcatgtgt ggaatttcag ccacgaacgc
      961 gcccgtgtag atcgtggcgg caacctcgac tggatcttcg gtgctgtagg ctctcgcctc
     1021 accaagaact tctcagacct agacctgatc ggggaagggg cgcagggacg catgtctggc
     1081 ttctacttta ccgatggaac ccagcacctc gaccacgaca cccagcagaa tcacatggct
     1141 ccgcactgca ccagcgacct tttgttcaaa ggtgcgctta aaggaaagag ccgctcggtg
     1201 tggcagggta tgatctatgt tgcccccggt gcccagaaga cggatggcta ccaagccaac
     1261 cgtaacctcg tcctcgatga ccaggcacgc gcggattcta ttccgggtct cgaaattctc
     1321 gccgacgatg tgcgctgtac acatggcgca accgttggta aacttgaaca ggaacccctg
     1381 ttttacctga aatctcgtgg tattccgcaa aaagaggcgg agcagttggt ggtggaaggt
     1441 ttctttgacc ccatcatgca acgcatcccg tttgaaggtg tgcgtgagcg cttccagcag
     1501 gcgattcaga tcaagttgac agcgtaattt gttttctctc gctggggctt acaccccagc
     1561 gagagcatta tcaccgtaag aaaatgcctg aagcgttgca tcaggcatcc tttaagaatg
     1621 aggcggaggg cgtctcaatt ttcgttcgta ggaagtgagc tatggcagtg aagtctaaaa
     1681 caaaatccaa ttcgaagaca aaagtggtgg taaaagcgaa aacggcaacc aaaaagggga
     1741 cggttaaggt tagtgcggct cgtaaagtag tttcaaaatc gaagacaaaa gtggttgcaa
     1801 aaagtgtcaa gcctagtaag tcggttgttt cttctataaa gaagaagcca gcttctgctg
     1861 aaaaaccggt tgaagtgacg cgcaccatta acttatcgag ccagatccgt cagcctgcga
     1921 aacctgtctt gcgccgctct tctgttgggt tgggtgcgac gaactccaat ccgttcacgt
     1981 ttatgaaggc gccacgccct gagtatgctt ttgaagtggg tgatacggta gaagtgttct
     2041 gtgatcacga aaagggacgc gaacgtgtgc gtggttggat caaaggcatt gtagttcaag
     2101 tggataacaa aatggtggca gtgcagttcc gctcgaacgt tttccttaca gacggttgga
     2161 tggtgccaga tcgcattctt tggtacccgc tgaattctga gcacattcgc cctggaacca
     2221 ccggcaagaa acccgccttg aagaaagata ttattccgga ttattaagcc aatacgcagg
     2281 tagacaggta cacaagcaaa aaatagacat gtgtacttgt gtacctgtct attttttatt
     2341 tcccatgacg ctaaacataa acgaaattcg taaagatttc cctattctag agcgcgaaac
     2401 tgccaatggc aagcgcgtga tttatcttga ttccaccgca acttctcaga agcctttgca
     2461 agtgatcgaa gcgatgaacg attattatcg ccgttcaaat gcaaatattc accgtggtgt
     2521 gcatactttg gctgaagaag ccacttcttt gtatgaaggt gcgcgtgaac gcattgccaa
     2581 atttatccat gcggcaactt cacgtgagat tatttacacg cgtaatacaa ctgagtcgat
     2641 taatcttgtc gcttattctt gggcgcgtgc aaatttaaag gcgggcgacc ttgttattct
     2701 gaccgagatg gaacaccata gcaatctggt tccgtggcat atgctgcaag cagagcgtgg
     2761 gattgagctg gaattcatcc ctgtgacaga agacggtttg ctggatctgg aagcttataa
     2821 aacgcttctc aaccggaccc cgaaactggt ctcgtttaca cacatgtcca acgtgttggg
     2881 cacgatcaac ccggcggcag agatcatcaa attagctcat gcggcgggtg ctgtcactct
     2941 agtggatggc gctcaatcag tccctcactt agccgtggat gttcaagcct tggatgctga
     3001 tttttatgcg ttctctgcgc ataagatgtg tggccccacc gggatgggtg tgttgtatgg
     3061 caagtcagct ttgttagaag ccatgcctcc gttccttggt ggtggtgaca tgatcaagga
     3121 agtaaaactg cgttccttcc gcccgaacac tttgccacat aaatttgaag cgggtacacc
     3181 tgccatcgca gaagccatcg gtttaggtgc tgctgttgat tatttgaccc agatcggcat
     3241 gagcgatatt gctgcccatg agcatgagat cactgaatat gcgcttgaac gcttggaaga
     3301 aatccctggt gtaaagctgt ttggtccctc tgcggataaa aaaggtggcg tagctgcttt
     3361 tacacttgaa ggtgtgcacc cccatgatgt ggcgcaaatt ttagatcagg atggcatcgc
     3421 cgtccgcgcg gggcatcact gtgctcagcc tttgcatgaa aagtttggca tccctgctac
     3481 cagcc
//
LOCUS       DAOQNJ010000252         3471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_18484_length_3471_cov_6.132611, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000252 DAOQNJ010000000
VERSION     DAOQNJ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18484_length_3471_cov_6.132611"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /gene="recJ"
                     /locus_tag="PLG52_08095"
     CDS             complement(<1..1371)
                     /gene="recJ"
                     /locus_tag="PLG52_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNB41447.1"
                     /translation="MDGSIFMTRWLDPQPVDIPASFQDLGLPPLIAQTLLRRGISSPV
                     EAEAFLYPEKNPNSQFPNIEKAVELLEMAIRNREKICVWGDFDVDGQTSTALLVQTLR
                     ALNADVMYYVPVRGKESHGIHIESLKPIIDNGAKLLLTCDTGITAHDAIDYANSRGLT
                     VIVTDHHDLGETLPNAQAIINPKLLPEDHPLRNLAGVGVAYKLAEALLDTRTSNIDSQ
                     ISNIELLDLVALGLIADVALLQNETRSLAKQGIEQLRNTSRLGLRVMAELAGAAFETL
                     TEETIGFTFAPRLNALGRLGDANPAIDLLITDDSARARVLATQIEGLNAQRRMLTKQV
                     NDAAEAQLKENPELLNHPIIILSHPNWPGGVVGIVANKLVERYHKPAILFNESEDGIL
                     RGSARSIEGLHITEAITTQKDLLIGFGGHPMAAGMALHKENLPAFRKGLGKAIEKQLG
                     DIVFEEP"
     gene            complement(1395..1595)
                     /locus_tag="PLG52_08100"
     CDS             complement(1395..1595)
                     /locus_tag="PLG52_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003224033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB41448.1"
                     /translation="MNNNLKVLRAEKNWSQADLAEKLNVSRQTVNALETGKYDPSLPL
                     AFKIADLFGKKIEEIFFPEKEN"
     gene            complement(1592..1981)
                     /locus_tag="PLG52_08105"
     CDS             complement(1592..1981)
                     /locus_tag="PLG52_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41449.1"
                     /translation="MNQDQVQKRYYKEFFIAIGAYVVILIGSISILKYLELPKSAQFI
                     VAILPIIPILFVIIAIMRALRDSDELQQKIQSYAIMFSAVATGLITFSYGLLENVGFP
                     PFPTIWVLPMMFMFWGFSLSYFWKKYQ"
     gene            complement(1986..2138)
                     /locus_tag="PLG52_08110"
     CDS             complement(1986..2138)
                     /locus_tag="PLG52_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41450.1"
                     /translation="MNTKNLGSTIAIAAGVGTAVSVSSHSIAVGVGVGVAIFIAFSVF
                     GNFKKE"
     gene            2270..2686
                     /locus_tag="PLG52_08115"
     CDS             2270..2686
                     /locus_tag="PLG52_08115"
                     /inference="COORDINATES: protein motif:HMM:NF022698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HNB41451.1"
                     /translation="MDTGYSGTPLYKKLGFTKAPLRVLTINVPREYKSWLGELPKGVG
                     LASNVRSPVQAAHVFATEKAFLDAVLSTLRTELAQDGFVWVSWPKKTSKVKTDITEDT
                     IRELALPLGFVDIKVCAVSEVWSGLKLVIRKSERKE"
     gene            2762..>3471
                     /locus_tag="PLG52_08120"
     CDS             2762..>3471
                     /locus_tag="PLG52_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB41452.1"
                     /translation="MKNVLKWIGIVVGSLVGLVLVAGFVMFLVGNARLNKTYDFPSSE
                     LVLPTDEASLEQGKHRVESLCAGCHGSDLSGIEKWFDAGPLGTIDSANLTSGDGGIGS
                     FYTTEDYVAAIRHGIDEEGKPIFMPAVTSTSQLSDEDLAAIIAYLKTVPPVDHKTNGK
                     NFTPLAKILFVVGVLPPLPVEVVSHDLHVTAPEAGPTAEYGEYMVNTNDCRVCHGPDL
                     NGGPFPDPTVTKISPNLTP"
BASE COUNT          863 a          734 c          923 g          951 t
ORIGIN      
        1 gggttcttca aagacaatat ccccaagttg tttttcaatc gctttgccaa gtcccttgcg
       61 gaaggcaggc agattttctt tgtgtaacgc catgcctgct gccatgggat gcccaccgaa
      121 gccgataagc aagtctttct gtgtggtgat ggcttcggta atgtgcaacc cttcaataga
      181 acgagccgag ccgcgcaaga tgccatcttc agactcgttg aaaagaattg cgggtttgtg
      241 ataccgctca acgagtttat tggcaacgat gcccaccaca ccaccgggcc agttaggatg
      301 cgaaagaatg atgatgggat gattaagcag ttcggggttt tcttttagtt gtgcctccgc
      361 cgcatcgttg acttgcttgg tgagcattcg gcgttgagcg tttagccctt caatttgagt
      421 agcaagcact cgcgcacgtg cagagtcgtc tgtaattaat aagtcaatgg cggggttggc
      481 atcacccaaa cgaccgagtg cgttgagacg tggagcaaag gtaaagccaa tcgtttcttc
      541 ggtgagtgtt tcaaatgcgg cgccagccag ttctgccatc acacgcaaac caaggcgact
      601 tgtatttctt agttgctcta tgccttgttt ggcaagtgag cgagtttcgt tttgaagcaa
      661 ggcaacatcg gctatcaagc caagagctac taaatcgagt aattcgatat tcgatatttg
      721 agagtcgatg ttcgatgttc gagtatcgag caacgcttca gctagtttgt acgcaacccc
      781 aacacccgcc aaatttcgca gtggatgatc ttctggcaga agcttggggt tgatgatggc
      841 ttgtgcattt ggcagggttt cacccaaatc atgatggtct gtaacgatga ctgttagtcc
      901 gcgagagttg gcgtaatcaa tggcatcatg agcagtgatg ccggtatcgc aagtgagcag
      961 tagctttgcg ccgttatcaa taattggttt gagcgactcg atgtgaatgc cgtggctttc
     1021 ttttccgcgc acgggcacgt aatacatcac gtctgcattc agggctcgca acgtttgtac
     1081 aagcagggct gtggaggttt gcccgtctac gtcaaaatca ccccagacgc agattttctc
     1141 tctattgcgg atagccattt caaggagttc gactgctttt tcgatattgg gaaattgaga
     1201 gttgggattc ttttcgggat aaagaaacgc ctcggcttcg acgggggagc tgattccgcg
     1261 tcggaggaga gtctgtgcga tgaggggagg caagcccaag tcttggaaag aggcggggat
     1321 gtctacaggt tgagggtcga gccagcgagt cataaaaata gaaccatcca aaaaagatgg
     1381 ttctattgta gcagttagtt ttctttttcc gggaaaaata tttcttcgat cttttttccg
     1441 aatagatcgg cgatcttgaa tgcaagcgga aggctggggt cgtacttgcc ggtttcaagg
     1501 gcgttgacgg tttgccttga gacattcaat ttctctgcaa ggtcggcttg agaccagttc
     1561 ttttctgcgc gcaagacctt gagattgttg ttcattggta tttcttccag aagtagctaa
     1621 ggctaaaccc ccagaacatg aacatcatgg gcaataccca aatggtaggg aagggcggaa
     1681 agcctacgtt ttcgagcaag ccatagctga atgtgatcaa gccggttgcc acggcagaga
     1741 acataatggc ataggactga attttttgct gaagttcgtc gctatcgcgt agtgcgcgca
     1801 tgatggcgat gatgacgaag agaataggga tgatgggcaa aatggcaacg atgaattggg
     1861 cagatttggg caattcgagg tatttaagaa tgctgatact gccgatcaaa ataaccacat
     1921 aggcaccaat ggcgataaag aactctttgt aatacctttt ttgaacttga tcctgattca
     1981 tttgattact cctttttgaa atttccaaac acgctaaatg cgataaaaat agcgacgcct
     2041 accccaacac cgactgcaat actgtgtgaa gagacgctga ccgcggttcc cactccggcg
     2101 gcgatggcga ttgtgcttcc tagatttttt gtgttcaagt tatatctcct tgtgtaaagt
     2161 ttgcttgact ttttgtattg taaagtaagc tttactttaa gtcaagggtt tttgactgac
     2221 acttttggag atgcgagtac acttaaaggg atacgaaagg attattggta tggacacagg
     2281 ttattcagga accccacttt ataagaaatt aggttttacc aaagcgccat tacgagtttt
     2341 gacgattaac gtccctcgcg aatacaagag ttggctgggg gaattgccaa agggtgtagg
     2401 gctggcttcg aacgtccgct cgcctgtgca ggcggcgcat gtgttcgcta cggagaaggc
     2461 ttttttagat gcggtccttt ccactttgcg aacggagctt gcccaagatg gttttgtttg
     2521 ggtatcttgg cccaagaaaa cctcaaaagt aaaaacagac attactgaag atacgatccg
     2581 tgagcttgct ttgccattag gttttgtgga tataaaagta tgtgccgtga gcgaagtgtg
     2641 gtctgggctt aagttggtga ttcggaagtc tgaacgaaaa gagtagttgc attcccatac
     2701 aaaattttgt accatgggga tgctcttaac gttattttat tgtcaaagga gaatgtgcaa
     2761 tatgaagaac gtattgaaat ggatcgggat cgtagtaggg agtttggtag ggctggtgct
     2821 tgtggcaggt tttgtcatgt ttcttgttgg gaatgcccgc ctgaacaaga cgtatgactt
     2881 cccatcctca gagcttgtct tgccgacgga tgaagccagc ttggaacagg ggaaacaccg
     2941 tgttgagtct ttgtgtgcag gctgccacgg atcagatctg agcgggatcg aaaagtggtt
     3001 tgacgcaggt ccgctaggga caattgactc tgccaactta accagcggtg acggcggtat
     3061 tgggagcttt tacacgaccg aagattatgt cgctgccatt cggcatggaa ttgatgagga
     3121 aggtaagcct atttttatgc cggcggtcac ttccacctca cagctaagcg acgaagactt
     3181 ggctgcgatc attgcttatc tcaagacggt tccgcctgtg gaccataaaa cgaatggcaa
     3241 aaatttcacg cctttggcaa agattttatt tgtcgttggt gttttgccgc ctctgccagt
     3301 ggaagttgta agccatgatc tgcatgtcac tgcgccagaa gccggaccca ccgctgaata
     3361 tggcgaatac atggttaata caaatgattg tcgcgtgtgc cacggacctg atctaaatgg
     3421 cggtcccttc ccagacccaa cggttacgaa gatctctcca aaccttactc c
//
LOCUS       DAOQNJ010000253         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_18579_length_3460_cov_7.724229, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000253 DAOQNJ010000000
VERSION     DAOQNJ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18579_length_3460_cov_7.724229"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PLG52_08125"
     CDS             <1..1025
                     /locus_tag="PLG52_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41453.1"
                     /translation="FALNLILFGLQYPHGRPDAWINWNVVARFIYLGGSNWQATFLRQ
                     WDHPDYPFLVPMTNAITWVFLGNASTWGPVALHFVLSLFAAGLLFAFVNIFRGFKQSI
                     LPVLIFTSLPFVVNQGMRQYSDFLVAYLILAAGGLTLMYLQTKEKRIAVLVGLVIGLA
                     GWAKNEGLMAILSVSFVWVLLALKKEGRTGLLRYVIGLVFPVVVIVLFKIFLAPSNDL
                     ISAQESLADKIFDIERYKTIFSIAIPMLWNLGNTPISIIGLIILTALLVRRSTNRITG
                     LWALAVIVLIQLTAYFGIFVLTPRDLAWHLNTSLDRLYLHILPLAFLLFFLWLKSPQE
                     LLSKES"
     gene            1029..1793
                     /locus_tag="PLG52_08130"
     CDS             1029..1793
                     /locus_tag="PLG52_08130"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNB41454.1"
                     /translation="MLLTIDIGNTNLTLGLYEGDTLGAHWRLSTDHNRMPDEYGLQFL
                     GVLQNAGKTIKDIQGISVASVVPPLTGRVIQACREYLKQEPLVVDAGVKTGIKIRYED
                     PKAVGADRVCDAVAVMKLYGGPACVVDFGTATTFNAVTRDGEYLGGAITAGINLAAEA
                     LYTHAAKLPRIDLQVPPSVIGRNTVHAMQSGLLFGYVSMVEGMVNRFRAELGSDMKVV
                     ATGGLAEVVAHETKVIDIIAPWLTLEGLRLIWEMNR"
     gene            1946..3184
                     /locus_tag="PLG52_08135"
     CDS             1946..3184
                     /locus_tag="PLG52_08135"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HNB41455.1"
                     /translation="MKTKKIFLTFVIVCTGLAACAPPPLSSTSNGSAPSTDSQPITDS
                     PQSVAPTESETHEAADANHSHTSDVDLTHLELGDGKYSTSPQMGYVYTCQTQFNGGGA
                     TGTGSWMNGDGTWDATKKAVVDGSITWPHSFTITIQGDQRVFVGNDLPDHPTGSYPIS
                     PSDDAYAVDRNPNSIQEQSVNFSVPANPTAAAQPNCIGGEVGILLSGVVVFNAFDAEG
                     RDAVAHEVQDECDGHPQKDGFYHYHSLSDCIEDTATSGHSSLVGYAFDGYGIYGYYGE
                     DGTEVTNEDLDECHGHIHVVEWDGQMVEMYHYHATHEFPYTVGCFHGTPSVRSLSGEG
                     GPQGQSQPAGQTQPTGQVGGVQGQQGQQPPQEAINACSGLSVGSACSINTPNGTLSGT
                     CGTPPNSTLVACMPAGGPPP"
BASE COUNT          841 a          841 c          844 g          934 t
ORIGIN      
        1 tctttgcgtt gaatctgatt cttttcggat tgcaataccc acatgggcgc cccgacgcgt
       61 ggatcaactg gaacgtggtc gcgcgtttta tctatctcgg tggttcaaac tggcaggcga
      121 catttcttcg ccaatgggat caccccgatt atccgttttt ggtgccaatg acgaacgcca
      181 ttacttgggt gtttttaggc aacgcatcca cttggggacc agttgcttta cacttcgtat
      241 tgtctctctt tgcagcagga ttgttgttcg catttgtaaa catttttcgc ggctttaaac
      301 aatccatttt accggttctt atattcactt cgcttccatt tgttgttaat cagggaatgc
      361 gtcagtattc agattttctg gtcgcttatc tcatcttagc cgcaggcggc ttgaccttaa
      421 tgtacctgca aacaaaagag aaacggattg ccgtccttgt tggtcttgtg attgggttgg
      481 caggctgggc aaagaacgaa ggactaatgg cgatcctgtc tgtgtctttt gtatgggttt
      541 tattggcttt gaaaaaagag ggacgaactg gattattaag atatgttatc ggtttggttt
      601 tccctgttgt agtgattgtg ctctttaaga ttttccttgc cccttccaat gacttaattt
      661 ctgctcaaga aagcttggcg gataagattt tcgatatcga gcgttacaaa accatcttta
      721 gtattgcaat ccccatgctg tggaatctgg ggaatacgcc tatcagcata attggactta
      781 taattttgac cgcacttctt gttcgtagat caacaaatcg tattacaggt ctatgggcgc
      841 ttgctgttat cgttcttatt cagttgacag cctatttcgg aatttttgtc cttacccctc
      901 gtgatctggc ttggcacctg aatacttctt tagatcgttt gtatttacac atccttccac
      961 ttgcatttct tttgttcttc ctctggctga aatctccgca agaactttta tcaaaagaga
     1021 gttgacccat gcttcttacg atcgacattg gcaataccaa ccttacgctt ggtttatacg
     1081 aaggtgatac gcttggcgca cactggcggc tctctacaga tcacaaccgt atgcccgatg
     1141 aatacggctt acagttttta ggcgtattgc aaaatgccgg aaagaccatc aaagatattc
     1201 aaggcatttc agtcgcatcc gtcgtcccgc cgttgaccgg gcgtgtgatt caagcctgcc
     1261 gcgaatatct taaacaagaa ccactcgtcg tggatgcggg tgtgaagacc ggcatcaaga
     1321 tccgttatga agaccccaaa gctgtcggtg cggaccgcgt ttgtgacgca gtggctgtga
     1381 tgaaacttta cggcggtccc gcctgtgtgg tggacttcgg cactgcgacc accttcaatg
     1441 cagttaccag agatggcgaa tatcttggtg gcgccatcac agcagggatc aatctcgctg
     1501 ccgaagcgct ttatacgcat gctgccaaac ttccgcgcat tgatttgcaa gtgccgcctt
     1561 ctgtcattgg gcgcaatacc gtccatgcca tgcaatccgg actcctcttc ggctacgtct
     1621 ccatggtcga gggaatggtg aaccgattcc gggcggagtt gggaagcgat atgaaagtcg
     1681 tcgcaaccgg cgggctggca gaagtcgtcg cacacgaaac caaagtcatc gatatcatcg
     1741 ccccctggct caccctcgag ggtctgcgcc tgatctggga aatgaaccgc tagaaatctt
     1801 gcacctgcca acgacccatg acagccgtca tgggtcgttg tgattcatct catgcttatt
     1861 gtaaaaaagc tgtgatttca cacactctgc atgtcaactc actagctata aactattgtt
     1921 aatcaattca ttagaggaga atgacgtgaa aacgaaaaag atatttttaa cgtttgtgat
     1981 cgtatgtact ggactggcgg catgcgcgcc tccgcctctt tcttcgacct cgaatgggtc
     2041 tgccccatca acagactctc agcccataac agattcgccc caatctgttg ctccaaccga
     2101 gagcgaaacc catgaagcag cagatgcaaa tcactctcat acttccgatg tggacttaac
     2161 tcaccttgaa cttggtgacg gtaaatattc caccagcccg caaatgggat atgtgtacac
     2221 ctgtcaaact caattcaatg gcggcggcgc gacgggcaca gggtcctgga tgaacggaga
     2281 tggcacctgg gatgcgacca agaaggcagt tgtcgatggc tcgatcacct ggccgcattc
     2341 tttcacaatc accatacagg gagatcaacg tgtgttcgtt gggaatgacc ttcccgacca
     2401 tcccactggc agttacccaa tttcacccag cgatgatgcc tatgctgtag accgcaaccc
     2461 gaactccatt caggagcaat cggtgaattt cagtgttccg gcgaacccaa cagcagcagc
     2521 gcagccgaac tgtattggtg gtgaagtggg tattctcctc tctggtgttg tggttttcaa
     2581 tgcatttgat gcggaagggc gcgatgctgt cgcccacgaa gttcaggatg aatgtgatgg
     2641 acaccctcaa aaagatggct tttatcacta ccatagcctg agcgattgta tcgaagacac
     2701 cgccaccagc ggacattctt ctctggttgg atatgctttc gatggctatg gcatctacgg
     2761 gtattatggc gaagacggca cggaagttac gaatgaagac cttgatgagt gccatggtca
     2821 tatccatgtt gtcgaatggg atggtcagat ggtggagatg tatcattacc atgcgacaca
     2881 tgaatttcca tatacagtgg ggtgctttca cgggacgcct tctgtgagat ctttatctgg
     2941 cgagggcgga cctcaaggtc agagtcagcc tgctgggcag actcagccca caggtcaggt
     3001 tggaggcgtg caaggacagc aagggcagca acctccgcaa gaagccatca atgcttgctc
     3061 aggtctatcg gttgggtccg cttgttccat caacaccccg aacggaacct tgagtggaac
     3121 ctgcggcacc ccgcccaatt caactttggt ggcgtgtatg cccgcaggcg gacctcctcc
     3181 ataaatataa acggactggc aatcgccagt ccgtttttca tgacgcgtgt catggggtgt
     3241 gatgatgaga tgcatgggtg ttgtgagaag atagtgaccc aatgcactct ttgtaaacgc
     3301 gctgcgccca taaaataaat tcaagattga acgtaaggag cgtatatgaa taaacaaaaa
     3361 gaatgcattt gccaactgaa cgacatttgt cgtaaccaaa ccatgagata aaaggagtta
     3421 acatgaaaaa caaaaaagta ttactcgtaa catgtgtcgc
//
LOCUS       DAOQNJ010000254         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_18857_length_3428_cov_8.890602, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000254 DAOQNJ010000000
VERSION     DAOQNJ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18857_length_3428_cov_8.890602"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="PLG52_08140"
     CDS             complement(<1..1640)
                     /locus_tag="PLG52_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNB41456.1"
                     /translation="MSDNTFYLGRLFDPKTAKLTSTDINYDSADLTTHAVVTGMTGSG
                     KTGLCISLLEEAALNGVPAIIIDPKGDLTNLLLHFPELLPKDFQPWIDPEVVRRANKT
                     PEQASQEAAMAWRNGLQEWGMTPERILDLKNSVDFAIYTPGSDSGIPVSVLSSLDAPG
                     LDWDSNREVLRERITSTVTALLGLVGMNDLDPIRSREHILLSNIFEEHWSQGNGIDLT
                     ELILQTQNPPFDKLGAFPVDTFFPPKDRMALAMTLNNILASPAFEAWREGQALDVQSL
                     LFKEDGTPRHSIFYLAHLSDGERMFFTTLLLSAVETWMRTQKGSAALRALLYMDEIYG
                     YLPPTANPPSKLPLLRMLKQARAFGLGILLATQNPVDLDYKALSNTGTWFIGKLQTER
                     DKNRLLDGLESLAGGFSRAEMDKLISSLGKRVFVMNNVHEKNPVLFQTRWAMNFLAGP
                     MTRTQIRDLNALVGAGEVAAPQPKPKPVSAPKVTAAPPPVDHVQPISVPSAPAQPKKT
                     PTRSTSSSVLESSNGGNLTKPPVPAGIREYYLPQNYSMP"
     gene            complement(1790..2956)
                     /locus_tag="PLG52_08145"
     CDS             complement(1790..2956)
                     /locus_tag="PLG52_08145"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HNB41457.1"
                     /translation="MTNLFDALEFCLREIEEGGTIETALARFPEHAEELRPILETSVK
                     AREMAVPEPSPNVVRRSRALVLQRAAELRESRVAPRSDSRRGFSLFQRLALSFALVAV
                     FLMSGGGLLSASASALPGEQLYTVKRGWENVRLFFIFDTQARALLEHEFENERLHEAN
                     ELILEGRHEVIQFAGVFMQVNGLTYVSGLPVTLPATIPLPANGDAVVVSGRTNSQGFV
                     EVINLEFLPAGSVVPVGKPIEVETESDTEQATGQTPTPSTGTSSGSTNEATPTPVKEY
                     EVKGTLEAVSTTSLVVTGMTVYLENSKIDGQMCVGVEVEVKGYYAQDGRFIVKEAKSK
                     GSCTSINNSGSNSGSSNNESGSNSSGESNSNGDDSGDDHGGNSGKDGGGGDDND"
     gene            complement(2949..>3428)
                     /locus_tag="PLG52_08150"
     CDS             complement(2949..>3428)
                     /locus_tag="PLG52_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB41458.1"
                     /translation="VYDQYFSEVYRFVLYRVGDQSLAEDIASDVFIRLLEAIKVGRGP
                     DSNLKGWLIGTASHVVTDHFRKKYRRPEDELSDSLPGLEPSPASEVDQREQNRLVRSA
                     YKKLTAEQQDVLALRFGQGYSLEETAARMDKNINAVKALQFRALAALQREIGEVDYD"
BASE COUNT          870 a          849 c          878 g          831 t
ORIGIN      
        1 ggcatgctgt agttttgcgg caaataatat tcgcggatac cagcaggaac gggcggcttg
       61 gttagattgc cgccattgga agattccaag acagaagacg acgtgctgcg tgtgggagtc
      121 tttttaggct gtgcaggcgc agaagggact gagatcggtt ggacgtggtc aacaggcggc
      181 ggggcggctg tcactttggg agcagagaca ggcttcggct tgggttgagg cgcagccact
      241 tccccagcac cgaccaaggc gttcaagtcg cgaatctgag tgcgggtcat gggacctgcg
      301 aggaagttca tcgcccaacg cgtttggaaa agcacgggat tcttttcgtg cacattgttc
      361 atgacgaaaa cgcgcttgcc gagggatgag atgagcttgt ccatttcggc gcgggagaaa
      421 ccgcccgcca aactttcgag cccatcgagc agacggttct tatcgcgctc ggtctggagt
      481 ttgccaatga accatgtgcc tgtatttgag agtgccttgt aatcaagatc aaccgggttt
      541 tgagtagcaa gcaaaatgcc caagccaaac gcacgagcct gcttcaacat gcgaagcaat
      601 ggaagcttgg aaggaggatt ggcagtggga ggaaggtagc cgtagatctc atccatataa
      661 agaagggcac gcaaagcagc ggagcctttt tgagtgcgca tccaggtttc aacggcagag
      721 agcaacaagg ttgtgaagaa catacgctcg ccatcggaaa gatgagcaag gtagaaaatg
      781 ctgtgacgcg gagtgccatc ttccttgaag agcaaggact gcacatccaa tgcctgacct
      841 tcacgccatg cttcaaatgc aggcgaagca aggatgttgt tgagggtcat cgccaatgcc
      901 atgcggtctt tgggcgggaa gaaagtatcg actgggaagg cgcccaattt atcgaaaggc
      961 gggttttgcg tctgcaagat gagctcggtt aggtcgatgc cattgccttg cgaccaatgt
     1021 tcttcaaaga tattcgaaag caaaatatgt tcacgcgagc ggatcgggtc gagatcattc
     1081 ataccgacca agcctagcaa ggctgtaacc gtgctggtga tgcgttcgcg caacacttcg
     1141 cggttggagt cccaatctaa accgggggca tcgagcgaag aaagcacact gacaggaata
     1201 cccgagtcag agccgggtgt ataaatagca aaatcaactg aattcttgag gtcgagaatt
     1261 cgttcgggag tcatccccca ttcttgcagt ccattgcgcc acgccatggc agcttcttgc
     1321 gaggcttgct caggtgtttt gtttgcacgg cgcacaactt cgggatcgat ccacggctga
     1381 aaatcttttg gcaataattc aggaaaatgc aggagcagat tggtcagatc ccctttgggg
     1441 tcgatgatga tggcaggcac accattgagc gctgcttctt caagaagcga aatgcataag
     1501 cccgttttcc cagaaccggt cataccggtg acaactgcat gggttgtcag gtcggctgag
     1561 tcatagttaa tatcggttga agtgagcttg gcagttttcg ggtcaaacag acgcccaaga
     1621 taaaaggtgt tgtcgctcat gtaatctctc ctatggatat aagacttttg aagtcttgaa
     1681 gttgtattga ttagaacgaa cgtgctagat gtttaattta accgatttta tgccaaaagg
     1741 caaatgattg cgaaagaaaa actgcgaggg gtttcctcgc agtttggtct taatcgttat
     1801 cgtcgccgcc gccaccgtct ttaccactgt tcccaccatg atcatcccct gagtcatcgc
     1861 cgtttgagtt gctttctcca gagctattcg aaccagactc attattacta ctgccagaat
     1921 ttgaaccact gttattaata ctcgtacaac ttcctttact ttttgcttct ttcacaataa
     1981 atctaccgtc ttgagcgtaa tagcccttca cttccacttc aacacccaca cacatctgcc
     2041 catcgatttt ggaattttca agatacaccg tcataccagt gacgacgaga gatgtagtag
     2101 ataccgcttc aagcgtacct ttgacttcat attcctttac cggagtaggt gtcgcctcat
     2161 tcgtggaccc agaactggta cctgttgaag gagttggggt ttgccctgta gcttgttctg
     2221 tatctgactc cgtttccact tcaattggtt ttccaaccgg cacgaccgag ccggcgggta
     2281 aaaactccaa gttgataact tctacaaagc cttgtgaatt cgtgcgtccg ctgacgacca
     2341 cagcatctcc attggcaggg aggggaatag tagcaggcaa ggtcacgggc aagccagaga
     2401 cgtatgtcaa cccgttcact tgcatgaaaa cacccgcgaa ctgaatcact tcgtgacgtc
     2461 cttcaagaat caattcattg gcttcatgca accgttcatt ttcaaattca tgttccaaca
     2521 aagcgcgggc ttgggtgtca aaaataaaga agagacgtac attttcccag ccgcgtttga
     2581 ctgtatacaa ttgctctccc ggcaacgctg aagcagatgc gctcagtaaa ccgccgccac
     2641 tcataaggaa gactgccacc aatgcaaaag aaagcgccaa gcgctgaaag agtgaaaagc
     2701 ctcttcgaga gtcactgcgt ggagcaactc tcgattcgcg taattctgcg gcacgttgta
     2761 acaccagggc acgagagcgt cgcaccacat ttggcgaagg ttcagggact gccatctccc
     2821 gggctttgac cgaagtttct aaaatcgggc gaagttcctc ggcatgttcg gggaagcgcg
     2881 ccagagcggt ttctatcgtt ccgccttctt cgatctcacg cagacaaaat tcaagagcat
     2941 caaataaatt agtcataatc tacctcgcca atctctcgtt gaagcgcagc cagcgcccgg
     3001 aattggagag ctttcacagc gtttatattt ttatccattc gcgcagcggt ttcctcgagc
     3061 gaataaccct gcccaaaacg taatgcaagg acatcctgtt gttcagcggt caatttctta
     3121 tatgcgcttc gcaccaaacg attctgttcg cgttgatcca cttcggaagc agggcttggt
     3181 tcaagccctg gcaatgaatc ggagagttca tcctctggac ggcggtactt ctttcggaag
     3241 tggtccgtca ccacatgtga ggcggtgccg atcaaccaac ctttgagatt ggagtctggt
     3301 ccgcgcccaa ctttgatcgc ttccaacaag cggatgaaca catcgctggc aatatcttct
     3361 gccaagcttt ggtcacctac acgatacaac acgaagcgat agacttcaga aaaatactgg
     3421 tcatacac
//
LOCUS       DAOQNJ010000255         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_18900_length_3424_cov_7.723360, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000255 DAOQNJ010000000
VERSION     DAOQNJ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18900_length_3424_cov_7.723360"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /locus_tag="PLG52_08155"
     CDS             complement(<1..1963)
                     /locus_tag="PLG52_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB41459.1"
                     /translation="MNSFKLSLINDQSSTPSKEFELTKSEIIIGREDSVDITIPASAV
                     SRRHARLMLIGENYVLEDLGSSNGTYVNGEKLDKQHTLKSGDKIRLGQIISLVYTAPV
                     SEEDSNLTVVSIKDANKTAKHSGPAKPAHVMQTVLGEAPAIPQASAGPRQLIVSIAGG
                     STQTYPLTGSSISIGRLDTNDIVIPSQIVSSRHARLEQVKGGGYKLVVMPEAKNPVQF
                     EGRPIDGSHVLHHNDLLRIGSMDPGVMVTITYNAPSEASQQMERDIVFGEKTLIQIGR
                     DPSNDVVIPSPNVSRFHTQIEKVGQRYRVEDLRSSNGTYVNGERIEGSVWLKPNDSIR
                     IGQYRFVMGKDQLARYDDGNGLRVDVVGLNKWVRKDLNILQNIYASFSPREFIVVVGQ
                     SGGGKSTFVDAVAGYRPATPPSKVYVNDIDIYTHFDAIRNEIGFVPQKDIIHMELTIY
                     QALDYAAQLRMPADTSPEERHKRIMEVLEDLDLTHRKDVQISGLSGGQQKRVSIGVEL
                     LTKPGLFFLDEPTSGLDPGTETALMQLMRRLADQGRTIILITHATKNVMLADKVIFLA
                     RGGYLAWFGPPDEALAYFDQYRSERARRAGKIEFDEIYAILDDPSKGKAEDWANRYRS
                     SNAHAEYVLKPLAGKLSPQSVATVPQPERL"
     assembly_gap    2173..2272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2845..3195)
                     /locus_tag="PLG52_08160"
     CDS             complement(2845..3195)
                     /locus_tag="PLG52_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41460.1"
                     /translation="MTVAAIHHTTAFPVILAKSADVAEANEIFHASHAALRLLLADFR
                     QGMIEADRQVLARRGRVPAALAQQLPAMLTRFAARLNELLGETSGLAPEQADILGALV
                     HAELLPWLSMTPFG"
BASE COUNT          807 a          817 c          812 g          888 t
ORIGIN      
        1 gaagtctttc gggctgtggt actgtcgcca cactttgtgg tgacaacttg ccagccaacg
       61 gttttaagac atattccgcg tgggcgttac ttgagcgata tcggttcgcc caatcttctg
      121 ctttgccttt gctgggatca tccaaaatgg catagatctc atcgaactcg atcttgccgg
      181 ctcggcgagc ccgctcagaa cgatactggt caaaataagc tagcgcttca tcaggaggac
      241 cgaaccatgc taaataacct cctcttgcga gaaagatcac cttatctgcc agcataacgt
      301 tttttgtggc atgtgtaatt aagatgatgg tacgtccttg gtctgccagg cgacgcatca
      361 attgcattaa cgctgtttct gtaccggggt ccaagcctga tgtgggttca tcaagaaaga
      421 acagaccggg tttggtcaaa agttcaacac caatcgagac gcgtttttgc tgcccgccgc
      481 tgagtccgct gatctgaacg tctttgcggt gggtcaggtc aaggtcttcc aaaacttcca
      541 taattcgttt gtgccgctct tccggggaag tgtcggctgg catgcgcaat tgagccgcat
      601 aatcaagtgc ctgatagata gtcaactcca tgtgaatgat atctttttgt ggcacgaacc
      661 caatctcatt gcggatcgcg tcaaaatgag tgtagatatc aatgtcattg acgtatactt
      721 ttgaaggagg agttgctgga cggtacccag cgactgcatc cacaaatgtg gatttgcctc
      781 cgccgctctg ccctacaaca acgatgaatt ctctggggct aaatgatgca tatatatttt
      841 gaagaatatt gagatccttg cgaacccact tattcaaacc aactacatcc actcttaagc
      901 cattcccgtc atcatatctg gcaagttgat ccttgcccat cacgaatcga tattgaccaa
      961 ttcggatgga atcgttgggc ttcagccaaa ccgagccttc aattcgttct ccattgacat
     1021 aggttccatt agaactgcgg aggtcttcta cccgatagcg ttgccctact ttttcgatct
     1081 gggtatggaa tcgagataca ttgggggagg ggatgaccac atcattgctg gggtcacgcc
     1141 cgatttgaat gagcgttttc tcgccgaaga cgatgtctcg ctccatttgc tgagatgctt
     1201 cactgggcgc attgtaggtt atagttacca taaccccagg gtccatactg ccaattctca
     1261 gcagatcatt gtgatggaga acatgtgagc cgtctatcgg acgcccttca aattgaaccg
     1321 gattttttgc ttcaggcatc accaccaact tgtacccgcc gcccttgacc tgctcaagcc
     1381 gcgcatgccg gcttgagaca atctgagatg ggatgacaat gtcgttggtg tcgagtctgc
     1441 caatggatat gctggaacct gttaacgggt aggtttgtgt gcttcctccg gcaatactaa
     1501 cgattagctg gcgtggacca gcacttgctt gcggtattgc tggtgcttca ccaagcacag
     1561 tttgcatcac atgtgcaggt ttagcaggac cggaatgttt ggcagttttg ttcgcgtctt
     1621 ttatgctgac aacagttagg ttggaatctt cttcagaaac gggagcggta taaaccaggc
     1681 tgataatttg ccccaatcga attttgtcac ctgattttag ggtgtgttgc ttatctagtt
     1741 tttccccatt gacatatgtg ccattactgc ttcccagatc ttccagtaca taattttcgc
     1801 caatcaacat cagtctggca tgacggcgtg acacagcaga tgcgggtatg gtgatatcca
     1861 cactgtcttc acgaccaatg atgatttctg actttgttaa ttcaaattct ttggaaggtg
     1921 tactgctttg atcattgata aggcttagtt tgaaactgtt cacttgagtc aaaggatgat
     1981 cctttcttgc aaattttcaa cacagtttct tgaatttatg gataagtgag aatttgtaca
     2041 gaattgagat aattaagaca agattatacc tttttattaa aatgcatgtc aacctaccaa
     2101 tgggggatac gtaaaatcac tgaaaatatt gtgtttgttt tcacttgctt tgctaaaaaa
     2161 ttggtctttt acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttttaatg
     2281 gtgaaaatat atgatgcaat cttcttcttt ttcccgaaag ccatctgaag aaagcaattc
     2341 gctggttcgt actctgcgga ggcgggcaga cttgcatcct gaaaggctgg cgtttagatt
     2401 tttgcaggat gatgaatcag aaaatatcac catcaacttt ggggaacttg atcgccgggc
     2461 acgagccatt ggggcatggt tagaaagctt caacgcaaaa ggggagcgtg cacttattct
     2521 ctatccacct ggattggatt acatcgctgc ttttatgggc tgcttatatg ctggtgtaat
     2581 cgctgtccct gcatacccgc ctcgccttaa ccgtcctgta ccccgtatcc aatctatcgt
     2641 tgacgattca catgcgacct ttgcgctgac tacaacgtcg atccattcga acatcgaaaa
     2701 acgatttgaa catgctccag acttgaaagc gctacattgg ttaaacaccg agcaggtacc
     2761 ggcggggttg gaagcggatt ggcgcgatcc taatgtctcg ccagatacat tggcattcct
     2821 tcaatataca tccggctcta ccagtcagcc gaaaggggtc atgctcagcc atggcaatag
     2881 ctcggcatgc accagcgcac ccagtatatc ggcctgctcc ggcgccagcc cgctggtttc
     2941 acccagcagc tcgttcaggc gcgcggcaaa ccgcgtgagc attgccggca actgctgcgc
     3001 cagtgcggcc ggcacccgcc cacggcgggc cagcacctga cggtctgcct cgatcatgcc
     3061 ttggcgaaaa tccgccaaca acagccgcag cgctgcatgg ctggcatgaa aaatttcgtt
     3121 ggcttcggct acgtcagccg atttggccag gatgacggga aaagcagtcg tgtgatgaat
     3181 ggcagcgaca gtcatgaaca acacctcaaa atcagttgcg atgttgtgcc agacgcgccg
     3241 gagagaaaaa tgtcagatca gggagcgcga tgtcagatgg cgcggcgatg catttgcggc
     3301 agtcgaggca aaaggccagg cgcccggcag cctgcgtgcc agcacgactg cagtcacggc
     3361 actcgactaa aatcgatcgc gtggtaaaca gcgcttatca ggcagcccta agaacgcaaa
     3421 gcag
//
LOCUS       DAOQNJ010000256         3419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_18942_length_3419_cov_7.976516, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000256 DAOQNJ010000000
VERSION     DAOQNJ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18942_length_3419_cov_7.976516"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /gene="pyrF"
                     /locus_tag="PLG52_08165"
     CDS             <1..557
                     /gene="pyrF"
                     /locus_tag="PLG52_08165"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNB41461.1"
                     /translation="GSRIPIILDAKRGDISSTAEAYAKSAFESLGADCITLNPYLGKD
                     SIDPFIGYKQKGVFLLCKTSNKGSGDLQDVTLSNGETMYIHVAKLAEAWNEKNNIGLV
                     VGATHIDAMKSIREAAPNLWFLAPGVGTQGGELDAALNAGLRADGKGLLLPMSRSISR
                     AENPGKAAAEVRDEILYWKNTNQK"
     gene            612..1220
                     /gene="pyrE"
                     /locus_tag="PLG52_08170"
     CDS             612..1220
                     /gene="pyrE"
                     /locus_tag="PLG52_08170"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNB41462.1"
                     /translation="MSSLPSHLTALADGLLDAGCIKFGEFTLKSGLKSPIYIDLRYII
                     SFPKLLETIGAAYLPLLKELNFKRIAGLPYAAIPIATAVSLQGNYPMIYPRKEVKTYG
                     TKAEIEGEYHAGETVVVIDDLATTGGSKFEAIEKLTGVGLVVKDVVVLVDRQSGAKES
                     LEQAGYNLHAVLTITQLLDYWEENGKVSKEKIAETRTFLKGS"
     gene            1224..1427
                     /locus_tag="PLG52_08175"
     CDS             1224..1427
                     /locus_tag="PLG52_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNB41463.1"
                     /translation="MTQDSMTIPLDLSSALSADAEAKALFDLLSPSHQREYIKWVEEA
                     KKTETRAKRIQKMMDMLKQGKEN"
     gene            1440..1985
                     /locus_tag="PLG52_08180"
     CDS             1440..1985
                     /locus_tag="PLG52_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB41464.1"
                     /translation="MAGIAVNVAVIQNGKILLTQRDDFETWILPSGGVEDGESLAQAA
                     LRETKEETGLDVALTGLVGTYTRISNMPPIHAVLFTATPIGGTITCQPGETIAVDWFS
                     FDQLPEPLSVGHKRRINDAVSGKKGFVVSQEIIFSSMPDKMTIRDLIELRDKSGFSRQ
                     DFYIKMMEDAILKETVEVMGF"
     gene            2141..3157
                     /locus_tag="PLG52_08185"
     CDS             2141..3157
                     /locus_tag="PLG52_08185"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNB41465.1"
                     /translation="MYNFLRPLLFRLNPERAHALTLHTLSFAGRFLPSRKVLEGLYQA
                     HSKPVTVFGLTFKNPIGLAAGYDKDGIAIRGLAALGFGHVEVGTVTPKPQPGNPSPRV
                     FRLVEDDAVINRMGFPSRGMEYLEQQLSGGANWNTVLGVNLGKNKETPNEEAVSDYLA
                     LLKKFAAYANYLTINVSSPNTVGLRSLQGREALEGLLTQLHQQRLAEQEKCKKRLPIL
                     VKLAPDLSDAELSDALEAILTTNMDGVIVTNTTLSREGLKSSYREETGGLSGSPLTVK
                     SEAVLRQTLKFVNGKIPVVSAGGVMNSEDAKRRLDMGAALVQVYTGLVYRGPGLVKEI
                     VRAL"
     gene            complement(3154..>3419)
                     /locus_tag="PLG52_08190"
     CDS             complement(3154..>3419)
                     /locus_tag="PLG52_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41466.1"
                     /translation="NIFTHKAEKAAPYESNCCYRDAAFSPDGTQVIFAFQDIRLAGNS
                     PINLYIIPADSISTPRKLEPILPMDFFTKRDDAPQPVMRAAQP"
BASE COUNT          933 a          688 c          890 g          908 t
ORIGIN      
        1 tggggtcacg cattcccatc atcctggatg cgaagcgtgg agatatttca tccacggcag
       61 aagcttacgc taaatcggca tttgaatctc tcggcgcaga ttgcatcact ttgaatccat
      121 atttgggtaa agattcaatt gacccgttta tcggctataa gcaaaaaggg gttttcttat
      181 tatgtaaaac ctcgaataaa ggctcgggag atttacaaga tgtgacatta tcgaatggcg
      241 agacaatgta catccacgtt gcaaaattag ctgaggcatg gaatgaaaaa aataacattg
      301 gtttggtagt gggggctacc catatcgatg ccatgaagtc tatccgtgaa gcggctccaa
      361 acttgtggtt tcttgcgccc ggtgtgggga cccaaggcgg tgagttggat gcagccttga
      421 acgctggctt gcgagcagat ggaaagggac tcttattgcc catgtcgcgt tctatttctc
      481 gtgcagagaa tccggggaag gcggcggctg aagtacgaga tgagattctc tattggaaga
      541 atacaaatca aaagtaataa aacgactact gttgaagatg accttcaaaa aactcaaata
      601 aaggaataac catgtcttct ttgccttctc accttactgc tcttgcggac ggacttctcg
      661 atgctggctg tatcaaattc ggcgaattta cgctcaagtc tgggcttaag tcgccgatct
      721 atattgacct acggtacatt atttcgtttc caaagctttt agagaccata ggggctgcct
      781 atttgccttt gttaaaagag ttgaacttta aacgcattgc tgggttgccc tatgccgcga
      841 ttccaattgc gacagccgtt tctttacaag gcaactatcc catgatctac ccacgcaaag
      901 aagtgaagac ctatggcacc aaagctgaga ttgaaggtga gtatcatgct ggcgagaccg
      961 tggtagttat tgacgatctt gctaccaccg gtggaagcaa atttgaagcc atcgaaaaat
     1021 tgactggcgt tggcttggtg gtaaaagatg tcgttgtact cgtggaccgt cagtctgggg
     1081 caaaagaatc actggagcag gctggctaca acctgcatgc tgtgctgacc atcactcaac
     1141 tattggatta ttgggaagaa aacggcaagg ttagtaaaga aaagatagca gagacgcgaa
     1201 cctttttgaa agggagttga cgtatgacgc aagactctat gaccattcca ctcgaccttt
     1261 cttcagcgct ttcagcagat gccgaagcaa aagccctgtt tgatctactt tccccttctc
     1321 atcaacggga atatatcaaa tgggtggaag aagcaaagaa aacagaaacc cgtgccaagc
     1381 gaattcaaaa gatgatggat atgttgaaac agggcaaaga aaattaagag gtacaaaaca
     1441 tggccggtat tgccgtaaat gtcgcggtaa ttcaaaatgg aaaaattctc ttaacacaac
     1501 gtgatgattt tgagacttgg atattaccca gtggtggggt cgaagatggg gaaagccttg
     1561 cgcaagctgc gctccgtgag acaaaagaag aaactgggct cgatgtagct cttacagggt
     1621 tggttggaac ctatacaaga ataagtaata tgccgcccat tcatgctgtt ttgtttacag
     1681 ctacacctat tggagggaca attacctgcc agcccggtga gacgattgca gtggattggt
     1741 tttcttttga ccaactccct gaacctcttt ctgttggaca taaaagaaga ataaatgatg
     1801 ccgtttctgg aaaaaaaggg tttgttgttt ctcaagaaat tatattttct tccatgccag
     1861 ataaaatgac gataagagat ttgattgaac ttcgtgataa atcgggcttt tcacgacaag
     1921 atttttatat aaaaatgatg gaagatgcaa tattgaaaga gacagttgaa gttatgggat
     1981 tctgaggaat tcagaaaatg attaagcttt attcaaaaat atttttagcc ttgattacat
     2041 tgttgtcttt ggcttgtaat cttttatcta ttccgcaaga ggcggaaact gtaaataccg
     2101 aacctgttaa gaacgaacag actcctaaca ttctataact atgtataact ttcttcgtcc
     2161 acttctcttt cgcctcaacc cagaacgcgc acatgcgttg accctgcata ctttaagctt
     2221 cgctggaaga tttcttcctt cacgtaaagt gcttgagggg ttgtaccaag cccattccaa
     2281 accagtcact gtgtttgggt tgacgttcaa gaatccgatt gggttggcgg caggatacga
     2341 taaagatggc atcgccattc gcggacttgc tgctctaggg tttggtcacg tagaggtggg
     2401 cacggtcacc cccaagccgc aaccgggaaa ccccagcccg cgggtgtttc gtttggtgga
     2461 agatgatgcg gttatcaacc gcatgggctt cccttcacgt gggatggaat atttggaaca
     2521 acagctttct ggtggcgcaa attggaatac ggttcttgga gttaaccttg gcaagaacaa
     2581 agagactccc aacgaagaag cggtctctga ttatttggca ttgttgaaaa agtttgccgc
     2641 atatgccaat tatctgacga ttaacgtcag ctcgccaaat acggtgggac ttcgcagctt
     2701 gcagggacgt gaagccttgg aggggttgct cacgcaatta catcaacagc gtttggcgga
     2761 acaagaaaag tgtaagaagc gtttgcccat tcttgtgaag ttggcgccag acctttctga
     2821 tgctgaattg agcgatgcct tggaagctat cctcactacg aacatggatg gtgtgattgt
     2881 cacgaatacc acattgagtc gtgagggatt aaagtcaagt tatcgggaag aaacaggcgg
     2941 gttgagtggc agtccgctta cggttaagag cgaggcggtt cttcgtcaga ccttgaagtt
     3001 tgtgaatgga aaaatcccgg tggtgagtgc gggcggagtt atgaattctg aggatgcaaa
     3061 acgccgcctc gatatggggg cggcgctggt gcaggtttat acggggttgg tctatcgcgg
     3121 acccggtttg gtgaaagaga tcgttcgcgc tctttagggt tgtgcagcgc gcatgacagg
     3181 ttgtggggca tcgtctcgtt tggtgaagaa atccatggga aggatgggct cgagtttgcg
     3241 cggcgtggaa attgagtcag cgggaatgat atagagattg attggactat tgcccgccag
     3301 acgaatatct tgaaaggcaa agatcacttg ggtgccatcg gggctgaagg cagcatcgcg
     3361 atagcaacaa ttgctttcat acggcgcggc tttttcggct ttgtgggtga agatgttgt
//
LOCUS       DAOQNJ010000257         3417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_18969_length_3417_cov_11.425640, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000257 DAOQNJ010000000
VERSION     DAOQNJ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18969_length_3417_cov_11.425640"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLG52_08195"
     CDS             <1..224
                     /locus_tag="PLG52_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41467.1"
                     /translation="LILKNSTSILIAHRLSTVKAADRIVVMRKGTIIEEGSHDGLLQQ
                     SGHYAELYNTYFRHQSLAYVEQMKEMVSK"
     gene            complement(262..942)
                     /locus_tag="PLG52_08200"
     CDS             complement(262..942)
                     /locus_tag="PLG52_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNB41468.1"
                     /translation="MLVLIAGGGRTGAQLAAQLLQQNYEVRLVEHRKELLARLHHELP
                     TEVIYEGQATDPNVLRQAGLEKANVIVACTNDDAANLVLCYLARTLFKVRRTVARINN
                     PRNSWLFDNNFHVDETINQADVMAHLIQEEMSLGDMMTLLKIRRGRYSLVEEKVPKGA
                     KAIGMQLKDLGLPEQCVIAAIIRDGQVTLPRGTTELEEFDEVLAITDDEGAKQLAHLL
                     EPPDRSVL"
     gene            complement(944..1609)
                     /locus_tag="PLG52_08205"
     CDS             complement(944..1609)
                     /locus_tag="PLG52_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNB41469.1"
                     /translation="MNFIVIGCGRFGAELAYKLFQNGHQVVVVDANPKAFNRLHPDFR
                     GRTVEGDSLSADTLSRASADKATGIAVVTNSDTMNAVIGHTIRVHYPNVEQVIVRNYD
                     PAMRDMLEAFGLQIVSSTAWGAERVQELLIDPSFRAVFSAGNGEVEMYEMYISPKWDG
                     MTISNLLDGCGEIVCSALTRAGRAQLPNGGMKLQKGDILTVSATLEGVKALRAKLQEG
                     KEA"
     gene            complement(1622..>3417)
                     /locus_tag="PLG52_08210"
     CDS             complement(1622..>3417)
                     /locus_tag="PLG52_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNB41470.1"
                     /translation="TQEILVILAAAGTMAFGYVFPISIAIVVLLAIVSISYEQTIHAY
                     PDGGGAYIVSRDNLGEFPALIAAAALLTDYILTVSVSIASGVAQLVSAFPSLYNLRVP
                     LAVLFILFIMLINLRGVKESGAAFAVPTYFFIVVILITIATGLWRLITGSLGMVLNPP
                     EIEFHGDAIATITPFLLLHAFSSGTAALTGIEAISNGITAFKEPRSKNAGVTLVWMSI
                     ILSTFFLGISFITNYVHAVPSETETVISQLARTIYDGRGTMYLMVISATTVILIMAAN
                     TAFADFPRLGALIAKDGFIPRQLAYRGSRLVYSRGIVTLAVIASILMVLFQASVTRLI
                     PLYAIGVFLSFTLSQGGMALRWWKSGRLKPGEQKVERGSTIHYEKNWVLKMFVNGFGA
                     VCTAVVMMVFAITKFKDGAYLVLVLIPMLVGVFWMIHRHYNNLAKNLSLDNFGVIPPQ
                     THRHRVIMPVSGVHQGTLAALRYARMLSDDVTAVHIVIEPDDAEKARKKWETWGEGVR
                     LVMLDSPYRLFVEPLLEYIADLAAKRQPGEIITVVVPEFVSENRWTAALHTNTADILR
                     NQLKNQHGIVITNVPYHVHEAEGDHADHVAH"
BASE COUNT          937 a          866 c          851 g          763 t
ORIGIN      
        1 atcttattct caaaaattca accagtatcc tcatcgcaca tcgtctctcc accgtcaaag
       61 ccgcagaccg cattgtagtg atgcgcaagg gaacgatcat cgaagaaggc agtcacgacg
      121 ggttgctcca acaaagcggg cactatgctg agctatacaa tacatacttc cgccatcaat
      181 cactggcata tgtagaacag atgaaagaaa tggtttcaaa ataagaaaaa acctcccgaa
      241 ttgtttcggg aggtttttgt tttacaacac agaacgatca ggtggttcca agaggtgggc
      301 taattgtttt gcgccctcat catcggtaat cgcaagcact tcgtcaaatt cttcaagctc
      361 ggtagtaccg cgcggaagag tcacctgtcc atcacggatg atggcagcga tgacacattg
      421 ctcaggcaaa cccaagtctt tgagctgcat accaatggct ttggcaccct tgggcacctt
      481 ctcctcgacc agtgagtagc gtccgcggcg gatcttgagc agggtcatca tatcgccaag
      541 agacatttct tcttgaatga ggtgagccat aacgtccgct tggttgatgg tttcatccac
      601 gtggaagttg ttatcaaaca gccagctatt gcgcggatta ttgatacgag ccaccgtgcg
      661 acggaccttg aaaagggtac gcgccaaata gcataaaacc aaatttgcag catcatcgtt
      721 ggtacaagcc acgatcacat tggctttttc caaaccagct tgacggagta cgttcgggtc
      781 tgtcgcctga ccttcataga taacttcggt aggaagttca tgatgcaagc gagccagcaa
      841 ttccttgcga tgttctacga ggcgcacctc gtaattttgt tggagaagtt gagcagccag
      901 ttgagcgccg gtgcgcccgc cgccagcaat aagtacaagc atgttatgcc tccttgcctt
      961 cctgcaattt tgcacgtaaa gctttcacgc cttccaaggt cgcgcttacg gtgagaatat
     1021 cacccttctg taatttcatt ccgccattcg gcaattgagc gcgtccagcg cgagtcaatg
     1081 cagagcagac aatctcgcca caaccatcca gcaagttgga gatggtcatg ccgtcccatt
     1141 tcggggagat gtacatctca tacatttcta cttcgccatt cccggcagag aataccgcac
     1201 ggaaagatgg gtcaatcaag agttcctgaa cacgctccgc accccatgct gtggagctga
     1261 cgatttgcag tccaaaggct tcgagcatat cgcgcatggc ggggtcataa ttgcggacga
     1321 tgacttgttc aacattcggg taatgcaccc gaatggtatg tccgataacg gcgttcattg
     1381 tgtctgaatt ggtgaccaca gcgatgccgg tcgccttatc ggcgctggcg cgggataagg
     1441 tgtcagcgga gagagagtca ccttccacgg tacgcccgcg aaagtctggg tggaggcgat
     1501 tgaacgcctt ggggttggca tccaccacca caacttgatg tccattttgg aaaagtttat
     1561 acgccagttc agcgccaaat cttccacatc caataacaat gaaattcata gtaaaaactc
     1621 cttagtgggc aacatgatcg gcatggtcgc cctctgcctc atgaacatga taaggcacat
     1681 tggtaatcac aatgccatgt tgattcttta attgattacg cagaatgtct gctgtattcg
     1741 tgtgcagggc agccgtccaa cgattctcgg aaacgaactc gggcaccacc actgtgatga
     1801 tctctccggg ttgacgtttc gctgcgaggt ctgcaatgta ttcaagcaac ggctctacga
     1861 acagacgata gggcgaatcc aacatcacca agcggacgcc ttcgccccag gtttcccatt
     1921 tcttgcgagc cttttcagca tcgtctggtt cgatcacaat atgcacagcc gtcacatcat
     1981 ctgaaagcat gcgcgcatag cgcaaagccg cgagagttcc ctgatggacg ccgctcaccg
     2041 gcataatcac gcggtgacga tgggtctgcg gtgggatgac gccaaagtta tctaacgaaa
     2101 gatttttcgc gaggttgttg taatggcgat gaatcatcca gaacacaccc acaagcatgg
     2161 ggatcaaaac taaaaccaga taggcgccgt ctttgaattt tgtgattgca aagaccatca
     2221 tcacgaccgc ggtacaaaca gcaccaaatc cattgacgaa catcttgagc acccaattct
     2281 tctcgtagtg aatggttgag ccacgctcta ccttttgttc tccgggcttg agccgtccac
     2341 tcttccacca gcgcaatgcc ataccacctt gagaaagggt gaatgaaagg aacacgccaa
     2401 tggcatatag aggaataaga cgggtcacac ttgcttggaa caacaccatc aagatcgaag
     2461 cgatcacagc cagagtcacg ataccgcggg aataaaccaa acggctgcca cggtaagcaa
     2521 gctggcgcgg aataaaacca tctttcgcga ttaaggcacc caaacgtgga aaatctgcga
     2581 aggctgtatt tgccgccatg atcaaaatga ccgtggtcgc agagatcacc atcaggtaca
     2641 tggttccacg cccatcgtaa atggtgcgag caagctgtga aataaccgtt tctgtttcag
     2701 aaggaacggc atgtacatag ttcgtaatga aggaaatgcc aaggaagaag gtgctgagga
     2761 tgatggacat ccaaaccaac gtcacaccgg catttttact gcgtggttcc ttgaaagcgg
     2821 tgatgccgtt cgagatggct tcaataccgg tcaacgcagc ggtaccgctg gagaaggcat
     2881 gtagcaacag aaacggcgta atggtagcaa tggcatctcc atggaactcg atctctggtg
     2941 ggttaagaac catacccagt gaaccggtaa taagacgcca taaacctgta gcaatggtga
     3001 taaggatgac aacgataaag aaataagtag ggacggcaaa cgctgcgcct gattccttta
     3061 caccgcgcaa gttaatcagc atgatgaaaa ggataaaaag caccgccaac ggcacacgta
     3121 ggttgtataa gcttgggaaa gcagaaacca attgagccac acccgaagcg atggacacag
     3181 acacagtgag gatgtagtcg gtcaacagcg ctgcagctgc gatcaatgct gggaattcac
     3241 ccaagttatc gcgcgaaacg atgtaagcac cgccgccatc ggggtaagcg tggatagtct
     3301 gttcgtaaga aatggacacg atcgccaaca acacaacaat ggcgatggat atcgggaata
     3361 cgtacccgaa tgccatggtg cccgccgccg ctaaaatgac gaggatttct tgagtgg
//
LOCUS       DAOQNJ010000258         3403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19089_length_3403_cov_7.786141, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000258 DAOQNJ010000000
VERSION     DAOQNJ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19089_length_3403_cov_7.786141"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLG52_08215"
     CDS             <1..343
                     /locus_tag="PLG52_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB41471.1"
                     /translation="SLSETPIITNQVPFSLADRSYVKNGILEYCQQNDILLTAYEPLN
                     QGNFRSTKTLEAIAKAHHTTVHQVALAWVVSHPRVITIPMSFNPVHIRENFEAAELEL
                     SADELEQLSST"
     gene            340..645
                     /locus_tag="PLG52_08220"
     CDS             340..645
                     /locus_tag="PLG52_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNB41472.1"
                     /translation="MNAHPLPEIQIIEETDTELEPMYSVIIHNDDVTPMDFVVEVLKQ
                     IFFLGNDRAAEIMLMAHIKGSAYVQSLPRAEAEKRVQRAHQAAGMQGYPLTFTVERE"
     gene            642..1505
                     /locus_tag="PLG52_08225"
     CDS             642..1505
                     /locus_tag="PLG52_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41473.1"
                     /translation="MTSNLETLTEINLDDLVSSFGWQDSPLLARLLRFFFRNPAQTFA
                     HHVVEYDDTVGAHGIVEGGWNLSRRYVQDIRIIGADRIPDSAFLALSNHPGMTDTVTL
                     FAALHRKDLRIIALDRPFLNALPHTTAQLFYVYDDPAKRMTLVRQVSAHLKKGGAILT
                     FPAGHIEPDPDIYPGAVESLKEWTDSVGVFIRMAPEAAILPVLVRNVVAKKYANHWLL
                     RIKKTKEEKEKLATALQLLGMVMFNEKPVTVTIQIGKPIYAKDLGTNETDAIHQAVLA
                     EMKSLIENPPK"
     gene            complement(1542..2831)
                     /locus_tag="PLG52_08230"
     CDS             complement(1542..2831)
                     /locus_tag="PLG52_08230"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB41474.1"
                     /translation="MHIPPSLKHKKFFYLWLGQLISITGTQMQLWALFWHINQIDKNP
                     IALGGIGVARILPVIIFSLIGGALADSVDRRKVMFATQSVAAVLALTLALFTQFGQVT
                     IWHIYIITALQAVVVAFDGPSRQALIPNLLPKEDLPNAFSMTFTAFQAGAVIGPALSG
                     FFIAGFGQEAVYYFNAISFVAVLVALLMIGDVPQAIGEKTAGISMESIKDGIRFILSK
                     PIIFSTMMVDFVATFFASASTLMPIIAKDVLDVGVVEYGYLSAASAVGAVAVALVISQ
                     VKEIRKQGLVFVVAVIIFGIATIIFGITRSFIVAWLAIAVTGGADGVSTIVRNTIRQL
                     QTPDHVRGRMTSINQIFFMGGPQLGEIEAGAVAQFFGAPIAVITGGIGCIVGTLLIIW
                     KWPQLREYNGDEPIEAGGAFPSPSTGASIIANRVRKQ"
     gene            complement(2900..>3403)
                     /gene="nadE"
                     /locus_tag="PLG52_08235"
     CDS             complement(2900..>3403)
                     /gene="nadE"
                     /locus_tag="PLG52_08235"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNB41475.1"
                     /translation="KLRKGNIMARERMIVIYDQSEVFKGLVVGTSNKTEILLGYSTLF
                     GDSANALNPIGDLYKTQVRQLSRAMNIPSPIVDKPPSADLWEDQTDEGELGFTYSEAD
                     RLLYLLVDQRYSPQEAIEAGFDEKFVNAVTTRIRRNQFKRMLPPIAKISNRTIGYDFL
                     YLRDWGT"
BASE COUNT          920 a          897 c          801 g          785 t
ORIGIN      
        1 gtcgctttct gaaacaccga tcatcaccaa tcaggtccca ttcagtctgg cagaccggtc
       61 atacgtcaag aacggcattt tggaatactg tcagcaaaac gacattttac ttaccgccta
      121 cgaacctctg aatcaaggga acttccgctc aacaaaaaca ttggaagcca tcgcgaaggc
      181 gcatcacacc acagtccatc aagttgcact ggcatgggtc gtatctcatc cgcgtgtcat
      241 caccattccc atgtctttta acccggttca catccgtgag aactttgaag ccgccgaact
      301 cgaattaagc gcagacgagc tggagcaatt atcaagtaca tgaacgcgca tcccctgcct
      361 gaaattcaga tcatcgaaga gaccgatacc gagcttgagc cgatgtactc cgtcatcatc
      421 cacaatgatg acgttacgcc aatggacttt gtggtggaag tgctcaagca gatattcttt
      481 ctcggcaatg accgcgccgc agagatcatg ctgatggcac acatcaaagg gtcggcatac
      541 gttcaaagcc tgccgcgtgc cgaagccgaa aagcgtgtgc aacgcgcaca tcaagccgca
      601 ggtatgcagg gctatccact cacctttaca gtagaacgcg aatgacatcc aacctcgaaa
      661 cccttaccga aataaacctc gacgaccttg tatcatcttt tggctggcag gattcgcctc
      721 tgcttgcccg cttgctgcgt ttcttcttcc gcaaccccgc gcagactttt gctcatcatg
      781 ttgtggaata tgacgatacg gtcggcgcac atggaattgt cgaaggtgga tggaatctct
      841 cgcgccgcta tgttcaagac atccgcatca tcggcgcgga ccggatccct gactcagcgt
      901 ttctagcctt atccaatcat cccggcatga ccgatacggt cacgcttttt gcagccctac
      961 atcgcaaaga cctgcgcatc atcgccttgg atagaccttt cctcaatgcg ctcccgcaca
     1021 ccacagcaca acttttctat gtctacgatg accccgccaa acgcatgacc ctggtgaggc
     1081 aggtcagtgc tcatctcaaa aaaggcggtg ccatcctcac cttccctgcc gggcatatcg
     1141 aacccgaccc agatatttat cccggcgccg tggaatcact taaagaatgg acagacagcg
     1201 ttggcgtgtt cattcgcatg gcgcccgagg ctgcgatctt gcctgtgctg gtgcgaaatg
     1261 tcgttgcaaa gaagtatgca aaccattggt tattgagaat caaaaagacg aaagaagaaa
     1321 aagaaaagct agcgacggca ctgcaattgc ttggcatggt gatgttcaac gaaaagcccg
     1381 taacagtcac catccaaatc ggaaagccaa tctacgccaa agacttgggc acgaacgaaa
     1441 cggacgccat ccatcaagcc gtgttggctg agatgaaaag cttgatagag aatcccccga
     1501 aataaaagag acagtcacct gcgaggtaac tgtctctttg tttactgttt ccgcactctg
     1561 ttggctatga tcgaagcccc agtagatgga cttgggaatg ccccacctgc ttctatgggc
     1621 tcatcaccat tgtattctcg tagttggggc cacttccaaa taatcagcaa ggttccaacg
     1681 atacaaccaa tgccgccagt aatgacggca atgggcgcgc caaagaactg cgccacggct
     1741 ccagcttcga tctcgccgag ctgcggtccg cccatgaaga agatctgatt gatactcgtc
     1801 atccgcccgc ggacgtgatc cggcgtttgc aactgacgga tggtattgcg tacgatggtg
     1861 ctgactccat cggcaccgcc ggtgacggca attgccagcc atgcaacgat aaatgacctt
     1921 gtaatcccaa aaatgatcgt tgcaattcca aagataatga cagcaaccac gaacaccagt
     1981 ccctgtttgc gaatctcttt aacctgtgaa ataaccaatg ctactgccac tgctcccacc
     2041 gccgaagcgg cggagaggta accatattcc actacaccta catccaatac atctttggca
     2101 atgatgggca tcaaggtgct ggcagaggca aagaaggtcg caacaaaatc aaccatcatg
     2161 gtggaaaaga taatcggttt ggagaggata aagcgaatgc cgtctttgat agattccata
     2221 ctgatgcccg ctgtcttttc gccaatggct tgagggacat ctccgatcat cagaagagcg
     2281 accaaaaccg ctacaaatga aatcgcattg aagtaataaa ctgcttcctg accaaagcca
     2341 gcaatgaaga agccactcaa ggcgggtcca atcacagcgc cagcctgaaa ggctgtgaat
     2401 gtcatactga aggcatttgg caaatcctct ttgggtaaca agttcggaat caacgcctga
     2461 cgagatggac catcaaacgc cacgaccacc gcttgcaatg cagtaatgat gtaaatgtgc
     2521 cagatggtga cctgcccaaa ttgtgtgaac aaagccaagg tcagagcaag aaccgcagca
     2581 acagactgtg tggcaaacat cactttacgt ctatccactg agtctgctag tgcaccaccg
     2641 atcaaagaaa agatgatgac gggcaaaata cgcgcgacac caataccacc aagcgcgatg
     2701 gggtttttgt caatctggtt gatatgccaa aataaagccc agagttgcat ctgggttccg
     2761 gtgatggaga tgagttgtcc gagccaaaga taaaagaatt ttttatgttt tagagaggga
     2821 ggaatatgca ttttattatt tgtaggggcg aggcgatacc acttcgtggc ataagccctg
     2881 cccctactcc attatttttt tatgttcccc aatcgcggag atataagaag tcgtagccga
     2941 tggtgcgatt gctgatcttg gcgatcggcg gaagcatgcg cttaaattga ttgcggcgaa
     3001 tgcgtgttgt gacggcgttg acaaatttct catcaaagcc agcttcaatc gcctcttgcg
     3061 gactgtagcg ttgatccacc aaaagataaa ggagtctgtc cgcttcagaa taggtaaagc
     3121 ctaattcgcc ttcgtcggtt tggtcttccc atagatcagc ggaaggtggt ttatcgacaa
     3181 ttggagatgg aatattcatc gcccttgaaa gttgacggac ttgcgtcttg tacaaatctc
     3241 caatgggatt aagagcattc gccgagtcac cgaagagagt gctgtacccc aaaaggattt
     3301 cagtcttatt gcttgtaccg acaactagcc ctttgaacac ttcgctttgg tcgtagatca
     3361 cgatcatccg ttcacgcgcc atgatgttgc ctttgcgcag ttt
//
LOCUS       DAOQNJ010000259         3400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19111_length_3400_cov_9.447235, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000259 DAOQNJ010000000
VERSION     DAOQNJ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19111_length_3400_cov_9.447235"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..538
                     /locus_tag="PLG52_08240"
     CDS             89..538
                     /locus_tag="PLG52_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB41476.1"
                     /translation="MSDSVQFSQSLRQWVDIFMHHSMRGWHHFVKSTGLTMPQFSILM
                     QLHYKGRIGMSEVSEQFGVSAAAASQLVDKLVQAGFMERAEDPSDRRAKLIQLSPNGK
                     KLIETGISERYRWMDGLTSKLSADDKAKVVDALQVLTEAARQLDETA"
     gene            635..2866
                     /locus_tag="PLG52_08245"
     CDS             635..2866
                     /locus_tag="PLG52_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41477.1"
                     /translation="MKFVKPYRWMLLFAIALIFAQANLDLALPDYLSRIVNTGIQQGG
                     VEDSMPEAIRASEMDKVAIFLNAQDKEDILASYTLVTDSSPDYETYLEKYPTLADEPI
                     YVLNDISQSEIDRLDPAMAKALLTVSGIEKALKDPEAAAQMGGAFGGFDPSKLPPGMD
                     VFTMLGKLPAAQLEQITSSVDEKFSALGDTMIVQAGVNVVRDEYKALGMDTEQIQNNY
                     ILTAGAWMIGLTLLSGVATIIVGYLSAKIAAGMARDIRRDVFQHVESFSSTEFDKFST
                     ASLITRTTNDVTQIQMVSMLMIRMMFYAPLMGVGGIIKVISNESPLAWIIGVAVLMLI
                     SLIAVVFSLSLPKFKIIQKLTDRINLVARENLTGMMVVRAFTMENFEEKRFDVANKDL
                     TSVSLFINRVMVVMMPLMMLIMNVMLLGVIWFGAHEVAASNMQVGDMMAFMQYSMQIV
                     MSFLMLSMMFIMIPRASVSADRIHEVLSTVPYIHDSTAAKKFPEPFKGEVEFRNVSFR
                     YPGGDIDAIQNVSFVAKPGQTTAFIGSTGAGKSTIVNLIPRFYEVSDGSIFIDGVDIR
                     DVTQHDLREKIGYVPQKSALFSGTIESNMLYADKNATKEMLDSAIEIAQAKEFVNEKP
                     EGMATEIAQGGTNVSGGQKQRLSIARAIVKKPPIYILDDSFSALDFKTDSALRRAFKE
                     KSKDSTLIIVTQRVSTIKNAEQIIVLDDGKIVGKGTHNELMETCETYKEIALSQLNME
                     ELA"
     gene            2863..>3400
                     /locus_tag="PLG52_08250"
     CDS             2863..>3400
                     /locus_tag="PLG52_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNB41478.1"
                     /translation="MMMQRPGQQQQGPRMGGPMGGPMMGAPGAKARDFKGTMKKLIEY
                     LGAYRNLIIVVFFFAVLSTAASIAGPKILGNATTKLFEGVMAELNGTGEIDFVYIGRI
                     ALITLGLYLVSALFSYIQGWIMANVSTDIAYRFRRDLSEKMNRMPLRYFDGTTHGEVL
                     SRITNDVDTLNQTLSQSLT"
BASE COUNT          865 a          915 c          794 g          826 t
ORIGIN      
        1 tctaataatt ttctaacgtc tcatcccttg accaaaactt aactaatgca taaagttaag
       61 cccgcttaac taaaaagaag gcttaactat gagtgactca gtccaattct cacaatctct
      121 gcgccaatgg gtggatattt tcatgcacca ctccatgcgc ggatggcacc acttcgtcaa
      181 atccaccggg ttgaccatgc ctcaattcag tattctgatg cagcttcact acaaaggacg
      241 cattggcatg tctgaggtga gcgagcaatt cggtgtttct gcagcggctg ccagccaact
      301 ggtggataag ctcgttcagg caggctttat ggaacgcgcc gaagacccca gtgaccgccg
      361 cgccaagttg attcagctct cccccaacgg caagaagctc atcgaaactg gcatctcaga
      421 acgctatcgt tggatggatg gtctcacatc taaactttca gcggatgaca aagccaaagt
      481 ggtggatgcg ctccaagtcc tgactgaggc ggcacgtcaa ctcgacgaaa ctgcataaag
      541 tctctcttcc ttctacaaat tttctacaca ttcttttgtt aaacatatcg tgatatttcg
      601 atattaagga gttttattcc gtatgatgaa gttaatgaaa ttcgtaaaac cttatcggtg
      661 gatgttattg tttgcgatcg ctttgatctt tgcgcaagca aacctcgacc ttgccctgcc
      721 cgattatctt tcgcgcatcg tcaacaccgg tattcaacaa ggcggtgtgg aagattccat
      781 gcctgaagcc attcgcgcca gtgaaatgga caaggtcgct atcttcttga acgcacagga
      841 taaggaagac atcctcgcct catacacact cgtaactgat tcttctcccg attacgagac
      901 ctacctcgaa aagtatccta ctctcgctga tgaacccatt tatgtgttaa atgatatttc
      961 gcagagtgag atcgatcgcc tcgacccggc aatggcaaaa gccttgctga ccgtttctgg
     1021 cattgaaaag gcgttgaaag acccagaagc cgccgcccaa atgggtggag ctttcggcgg
     1081 tttcgaccct agcaaactcc cccctggcat ggatgtgttc accatgctcg gaaaacttcc
     1141 tgctgcacag ttagagcaaa tcaccagctc agtggatgaa aaattctctg cgcttggcga
     1201 tacgatgatc gtgcaggcgg gcgtaaacgt ggtccgtgac gaatacaaag cgctcggcat
     1261 ggatacggaa cagatccaga acaactacat cctcaccgcg ggtgcatgga tgattggtct
     1321 tactctgctc tctggtgtgg caaccatcat tgttgggtat ctttctgcaa agatcgccgc
     1381 aggcatggcg cgtgacatcc gccgcgatgt gttccagcat gttgaaagct tctctagcac
     1441 cgagtttgat aagttctcaa cagcttcgct tatcacccgt actaccaacg atgtgaccca
     1501 gattcaaatg gtcagcatgt tgatgatccg catgatgttc tacgctccgc taatgggtgt
     1561 gggcggcatc atcaaagtca tcagcaacga atcaccgctg gcgtggatta tcggcgtggc
     1621 agtgttgatg ctaatcagcc tcatcgctgt cgtcttctcg ctctcgctgc ctaagttcaa
     1681 aatcatccaa aagctgaccg accgcatcaa ccttgtggca cgcgaaaacc tgaccggtat
     1741 gatggtcgtc cgcgctttca ccatggaaaa cttcgaagaa aaacgcttcg acgttgccaa
     1801 caaagacctg acctccgtca gcttgttcat caaccgcgtg atggtggtaa tgatgccgtt
     1861 gatgatgctc atcatgaacg tgatgctttt gggcgtaatc tggtttggtg cgcatgaagt
     1921 tgccgcctcg aacatgcaag tcggtgacat gatggcattc atgcagtatt caatgcagat
     1981 cgtaatgtcc ttcctgatgc tctcgatgat gttcattatg atcccgcgtg cttctgtttc
     2041 tgcagaccgc attcatgaag tgctgagcac cgtgccgtac atccacgatt cgacggcagc
     2101 caagaaattc cctgaacctt tcaagggtga agttgaattc cgcaacgtct cattccgcta
     2161 tccgggtggt gacattgatg cgatacaaaa cgtcagcttt gtggccaagc ccgggcagac
     2221 cactgcattc atcgggtcaa ccggagccgg aaagtccacc atcgtcaacc tcattccgcg
     2281 cttctacgaa gtttcggatg gctccatctt cattgacggc gttgacatcc gcgatgtgac
     2341 tcagcacgac ctgcgcgaaa aaatcggcta cgtgccgcaa aagtctgctt tgttctcggg
     2401 caccatcgaa agcaatatgt tgtatgccga taagaacgct acaaaagaaa tgcttgattc
     2461 tgccattgaa attgcacaag caaaagaatt tgtgaacgaa aaacctgaag gcatggcaac
     2521 cgaaatcgcc caaggcggta cgaacgtctc tggcggacag aaacagcgtc tctccattgc
     2581 ccgtgccatt gtcaagaaac cgccgatcta tattctcgac gatagcttct cggcactcga
     2641 tttcaaaacc gactcggctc tgcgccgcgc cttcaaagaa aaatcaaaag acagcacgct
     2701 gatcattgtt actcagcgcg tctcgaccat caagaatgcc gaacagatca ttgtgctcga
     2761 tgatggcaag atcgtcggca aaggaacaca caacgaactt atggaaacct gcgaaacata
     2821 taaagaaatt gctctctcgc agttgaacat ggaggaatta gcatgatgat gcaacgtcct
     2881 ggtcaacagc aacagggtcc gcgcatgggt ggacccatgg gcggtccgat gatgggtgcg
     2941 cccggcgcaa aagcccgcga tttcaaaggc acgatgaaaa agctcatcga ataccttggt
     3001 gcatatcgca acttaatcat cgtcgtcttt ttctttgcag tgctctccac cgccgccagc
     3061 attgccggtc cgaagatcct cggcaacgcc accacaaaac ttttcgaagg cgtgatggcg
     3121 gagttgaacg gtacaggcga aattgacttc gtctacatcg gacgcatcgc tctcataacg
     3181 ttgggcttgt atctcgtctc tgcgcttttc tcctacatcc aaggctggat catggcaaat
     3241 gtctcaaccg acattgccta tcggttccgc cgcgacctct ctgagaaaat gaaccgcatg
     3301 ccgttgcgct acttcgacgg caccacccac ggtgaagtgc tctctcgcat caccaacgat
     3361 gtggatacgc tcaaccaaac cctcagccaa agcctgaccc
//
LOCUS       DAOQNJ010000260         3395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19149_length_3395_cov_7.591617, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000260 DAOQNJ010000000
VERSION     DAOQNJ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19149_length_3395_cov_7.591617"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLG52_08255"
     CDS             <1..721
                     /locus_tag="PLG52_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41479.1"
                     /translation="DVPADLVDEQNDTALHQQLWEYFSTVIPLEARPTLAEFSIMTDG
                     SDNVLAAVAQTYDDPNRWSLEVDVADTSDYYYLTFTLVHEYAHLLTLGPDQVPPSLAV
                     FNNPDDNDLYLQEVAACATFFPGEGCSNPDSYMNAFYDQFWTNIYDEWNQINLEEDED
                     LYYEKLDDFYSKYEDQFLTDYSATNPAEDIAESFGFFVFADKPDGDRIAEQKILFFYQ
                     YPELVELRTKILNNVCVNFPQ"
     gene            725..1288
                     /locus_tag="PLG52_08260"
     CDS             725..1288
                     /locus_tag="PLG52_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HNB41480.1"
                     /translation="MKHLPIIESYWVEEGHFLAGEYPGSYNPEDARRRMDAFLESGIR
                     TFIDLTQPHELVPYEPILKEEARIYEHMAVYHRFSIRDHGIPSVQTMTLILDTIDAAI
                     ESGSPVYVHCWGGVGRTGTVVGCYMVRHGLTPSQALERVNALYKTRPQNYFLVRSPET
                     EEQADFVRKWRETPGTGHKAKQRFCEG"
     gene            1488..2075
                     /gene="lepB"
                     /locus_tag="PLG52_08265"
     CDS             1488..2075
                     /gene="lepB"
                     /locus_tag="PLG52_08265"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNB41481.1"
                     /translation="MDTLEQEPLVEESKPAEETTNWKRFVLDVIETLVLAVILFVGIN
                     SVSARVRVDGFSMQPTLQDGEFVLVSKLSYKFGDFQRGDIIVFDFPMNPDEELVKRII
                     GVPGDHVMVRDSHVYVNEQMLNENYIAQAPLYSGDWIVPDGTLFVLGDNRNNSNDSKD
                     WGLLPQGNVVGKAVLIYWPPKFWKVLDHPEMAVSQ"
     gene            2084..2371
                     /locus_tag="PLG52_08270"
     CDS             2084..2371
                     /locus_tag="PLG52_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HNB41482.1"
                     /translation="MNELNALTPCNECHAGLLHPRHVTYFTWLGHELITVPHFPAWVC
                     DVCGRREYDERAIQWLNMILDPNAGKPTPSHRNTPPRPRPQTGFRSPTPES"
     gene            2386..2943
                     /locus_tag="PLG52_08275"
     CDS             2386..2943
                     /locus_tag="PLG52_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41483.1"
                     /translation="MSLDSTSPRTYRFVLADILTSGYRVAGKVAVTTHGAMGFLNDIN
                     HSTLEIHDARMARLHMPTKLVDHFELVRTMKSRIHAVCLPRREDLGPQGIVRGGYMNV
                     VEYQVRLTTSMFEVDGIMELPGRFDFAGLISDGTREFLPIFNATVTAILIPSLRVQSA
                     GVLINRKMVDLVALLNQRVKPETHP"
     gene            2997..3069
                     /locus_tag="PLG52_08280"
     tRNA            2997..3069
                     /locus_tag="PLG52_08280"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3031..3033,aa:Glu,seq:ttc)
BASE COUNT          831 a          786 c          903 g          875 t
ORIGIN      
        1 ggatgtgcct gcggatcttg tggatgagca aaatgacacg gcgttgcacc aacaattgtg
       61 ggaatatttt tctacagtga ttccgctgga ggctcgccct accttggctg aattttccat
      121 catgaccgat ggctcggata atgtcttggc agccgtcgcg cagacctacg atgacccgaa
      181 ccgttggtct ttagaagtgg atgttgctga caccagcgat tattactacc taacctttac
      241 tcttgtgcat gaatatgccc atttgctcac tttaggtcca gaccaggttc cgcccagcct
      301 tgctgtgttc aataacccag atgataacga cctgtatttg caggaggtgg cggcttgtgc
      361 cacattcttc cctggggaag gttgctcgaa ccccgattcg tatatgaacg ctttttatga
      421 ccaattctgg accaatattt acgatgagtg gaatcagata aacctcgaag aagacgaaga
      481 tctttattac gaaaaacttg atgatttcta cagcaaatac gaagaccagt tcctgacaga
      541 ttactctgcg accaaccccg ctgaggatat tgctgagtcc tttggtttct ttgttttcgc
      601 agacaagcca gatggagaca gaattgcaga acaaaagatt ttgttcttct accaataccc
      661 tgaactagtg gaactgcgca caaaaattct caataacgtg tgtgtgaatt tcccacaata
      721 gcgtatgaaa catcttccca tcatcgaatc ctattgggtg gaagaaggtc attttctagc
      781 gggcgaatac ccgggcagtt acaaccctga agatgcccgc cgccgaatgg atgctttcct
      841 tgaaagcggt attcgcacat ttattgacct gacccaaccg catgagttgg ttccatacga
      901 accgatcctt aaagaggaag cgcgtatcta tgaacatatg gcggtttatc accgcttctc
      961 cattcgcgat catggtatcc cttcggtgca gacgatgacc ttgattctcg ataccatcga
     1021 tgctgcgatt gaaagcggca gcccggtgta tgtccactgt tggggtgggg tagggcggac
     1081 cggcaccgtg gtgggatgtt atatggtgcg gcatgggttg actccctcgc aagccttaga
     1141 gcgcgtaaat gctttgtata agacccgccc gcaaaattac tttttggtgc gctcgcccga
     1201 aacagaggaa caggcagatt ttgtccgcaa gtggcgagag acgccgggaa ctgggcataa
     1261 agcaaagcag cggttttgtg agggttaaga ctccattgca ggtgcaagtt ttgctttaag
     1321 gtggtgctac gattggcgga tcaaagctag agtgtggtaa aagccgaatg gcagctgccc
     1381 actcgacatc tcgcatgaag cggggtttca tccccaataa gtaagagtta agcatgggga
     1441 ggctctctgg aaatggcagt ataatttccg ggcagtttgg aggcattttg gatactttgg
     1501 aacaagaacc gttggtagag gaatcaaaac cagcggaaga aacaacaaat tggaagcgct
     1561 tcgttctaga tgtgattgag accttggtgc tggcagtgat cttatttgtg ggcatcaatt
     1621 ctgtttcggc acgtgtgcgg gtggatggct ttagtatgca acccacgttg caagatggtg
     1681 agttcgtgtt ggtgagcaag ttgagctata agttcggtga ttttcaacgc ggcgatatca
     1741 ttgtgttcga cttcccaatg aacccggatg aagaattggt gaagcgtatt attggggtgc
     1801 ccggtgatca tgtgatggtg cgcgatagcc atgtatatgt gaacgagcag atgttgaacg
     1861 agaactatat tgcccaagcg ccgctctatt ctggcgattg gatcgtgccg gatggaacct
     1921 tgttcgtgtt gggtgataat cgtaataact cgaacgactc aaaagactgg ggcttgctgc
     1981 ctcagggcaa tgtggtaggt aaggctgttt tgatctattg gcccccgaaa ttttggaaag
     2041 tgttagatca tccggagatg gctgtttcac aatagggttt gccatgaacg aattgaatgc
     2101 attaactcct tgtaacgaat gccatgcggg tttgctgcat ccccgccatg tgacttactt
     2161 cacctggctc gggcatgagt tgatcaccgt accgcatttt ccggcttggg tgtgtgatgt
     2221 gtgcgggcgg cgcgagtatg atgaacgcgc gatccaatgg ttgaacatga tcttggaccc
     2281 gaatgctggc aagcctacgc cttcgcatcg caacaccccg cctcgccctc gacctcaaac
     2341 agggttccgc tccccgaccc ctgaatctta actcaaggtt tctccatgtc acttgatagc
     2401 acttctcctc gtacatatcg ctttgtgctg gcagatatcc taacttctgg gtatcgtgtg
     2461 gcaggcaagg ttgcagtgac cactcatggc gccatggggt tcttaaacga tatcaatcat
     2521 tcaacgctgg aaattcacga tgcgcgtatg gcacgtttac acatgcctac caagttagtg
     2581 gatcatttcg aattggtacg cacgatgaag agccgcattc atgctgtttg tttacctcgg
     2641 cgtgaagacc tgggtccgca agggattgtg cgtgggggat atatgaatgt agtggaatat
     2701 caggtgcgcc tgacgacttc catgtttgaa gtggacggta tcatggagtt acccggtcgc
     2761 tttgattttg ccgggctgat ctcagatggc acgcgtgaat tcctgccgat tttcaatgct
     2821 acagtgacgg cgatcctcat accaagcttg cgagtgcaaa gcgcaggcgt gctgattaac
     2881 cgcaaaatgg tagacctggt ggcgcttttg aaccagcgtg tgaagccaga aactcatccc
     2941 taaaatttct tgacgcatcc cgaagtggat gataaaatct cgcccgctta agaaatgccc
     3001 ccatcgtcta gtggactagg acgcagccct ttcaaggcta cgaccggggt tcgaatcccc
     3061 gtgggggcac aacctaaaag caaccgtctc gtaagaggcg gtttttagtt taaaaagcca
     3121 gactcatttt tggtataatc ccgtcagttc gaaatcaaag acaagcgcaa agataagtag
     3181 ttcagcaacg gtacagagag ccgccggtga gtgcgaggcg gaccgccata ggatgaaatc
     3241 cactttgccc agtcattctc ggagagacgc aagtcataag agaatcggga gcgcccgtta
     3301 gcgcgcatcc aaggttatgc ttcggcatag cgaaagcagg tggcaccacg agacgcccct
     3361 cgtcctgcaa acggaatggg gcgtatttta tttaa
//
LOCUS       DAOQNJ010000261         3389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19200_length_3389_cov_8.957409, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000261 DAOQNJ010000000
VERSION     DAOQNJ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19200_length_3389_cov_8.957409"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLG52_08285"
     CDS             <1..796
                     /locus_tag="PLG52_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB41484.1"
                     /translation="PSLKLFVGAFTDDAGRFTLANMLALFTQQSILDSYKTSIQISAV
                     TAIGGGFFGFLLAYSITVGGLPRSLRSMLITFSGVASNFAGVPLAFAFVATLGRTGII
                     TAFLDEFVFQKFFGVKLYQTGFNLYSFLGLSLTYMYFQFPLMVLIITPALDGLKREWR
                     EAAENLGATQFQYWSKVAMPILLPPLLGATILLFGNAFGAYATAYALTGGTLPIVTIQ
                     IGAQIRGDVLHNPGLGYAMAMGMVIIMAISITGYTWLQKISERWLK"
     gene            793..1623
                     /locus_tag="PLG52_08290"
     CDS             793..1623
                     /locus_tag="PLG52_08290"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41485.1"
                     /translation="MKKRSSRNFPFWSWVWFILGALYFILPLYATLDFSLQMERDVIG
                     FKAYEKAFEDKDFIETFTYSNILAFATIAVSIILIVPTAYWIRLRVPEARRAVEFITL
                     LPFVVPGIILVFGLIRIYSSPLTIPFTDIVLMQPLTTYKFGTNVLIVAGYMVLAMPYM
                     YRSVDTGMRTVDVATLTEAAQSLGASWFTIITRIILPNIRSALLSGALLTFAIVVGEV
                     TLASFLGVDAFGPYLFLLGQHRAYEPAALSFVSFLLTWAAMGLIQLFTGGQGQTAGAH
                     "
     gene            1643..2704
                     /locus_tag="PLG52_08295"
     CDS             1643..2704
                     /locus_tag="PLG52_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41486.1"
                     /translation="MAFLSISNVSKQYGDATVVDNFNLDIEKGEFVSFLGPSGCGKTT
                     TLRMVAGFEIPTMGKIILDGADITNKAPNQRNVGMIFQSYALFPNMTVAQNIGFGLRV
                     RKEPEETVKKRVDEMINLINLEKHAHKYPYQLSGGQQQRVSLARALANRPRVLLLDEP
                     LSALDAKIRVSLRTEIRSIQKSLGITAIFVTHDQEEALSISDRIVVMNVGVIEQVGTP
                     FEIYNFPQTQFVANFVGSLNTAKAEVTDPANGLLTVDGVQFVTASDIKSRKKGDAIRV
                     SIRPERFSFADVQKKDNVLDCTIENITFLGSVVRIQVKIGESKFNMDTFNNPFLELPK
                     IGSKEQVTCSKEAVLVLDE"
     gene            2756..>3389
                     /locus_tag="PLG52_08300"
     CDS             2756..>3389
                     /locus_tag="PLG52_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNB41487.1"
                     /translation="MSKAILVLSDALRYDVAISNMGYLGHMLEYKKASLYKIVGELPS
                     MSRPMYETIHTGMPSSETGIVANSIVRLSEKPNIFKSVREAGKVTAAAAYYWYSELYN
                     RAPYNPIDDREVDDASLNIQHGRFYTTDEYPDIELFNTAGHLVRKYSPDYLLLHPMGM
                     DYHGETYGSDTKEYRNHAIYQDSKLAPLLVEWRERGYTIFVTGDHGINADG"
BASE COUNT          793 a          785 c          821 g          990 t
ORIGIN      
        1 gccctctctt aaattatttg ttggggcatt taccgatgat gctgggcgtt ttacgctggc
       61 gaatatgcta gcattgttca cccagcaatc cattcttgat tcctataaaa cgagcattca
      121 gatcagcgca gtcactgcga ttgggggcgg ctttttcggg ttcctgctgg catactccat
      181 cactgtgggt gggttgccgc gatctctgcg gtctatgttg attacatttt caggcgttgc
      241 atccaacttt gcaggcgtgc ctttggcatt cgcctttgtt gctacgcttg gacgcacggg
      301 tatcatcact gcttttctgg atgaatttgt atttcaaaaa ttttttggcg taaaactcta
      361 ccaaaccggt ttcaatctat atagcttcct cggtttgagt ctcacttaca tgtattttca
      421 atttccattg atggttttga tcattacccc ggcattggat ggtttgaaac gtgaatggcg
      481 tgaagctgcc gaaaatttgg gcgcgaccca atttcaatat tggtcaaagg ttgccatgcc
      541 gatattactt ccgccattgt tgggtgcaac cattttgttg ttcggaaatg cgtttggtgc
      601 gtatgccaca gcctatgcat tgacgggtgg caccctgcca attgtgacga tccagatcgg
      661 ggcgcagatc cgcggcgacg ttcttcataa tcccggtttg gggtacgcca tggcgatggg
      721 tatggttatc atcatggcga tttccatcac ggggtatacc tggctacaga aaatatctga
      781 aaggtggttg aaatgaaaaa acgatcatca cgaaactttc ctttttggag ctgggtttgg
      841 tttattttag gcgcactgta ttttattttg cccctatatg ccactcttga tttttccctt
      901 caaatggagc gcgatgtcat tggtttcaaa gcgtatgaga aagcctttga agataaagac
      961 ttcattgaga ctttcacata ttccaacatt ttggcgtttg caaccattgc tgtcagtatt
     1021 attctgatcg tcccaaccgc atactggatt cgcttgcgtg ttccagaagc gcgccgtgct
     1081 gtggaattca tcaccttgtt gccgtttgtg gtgcccggaa ttatcttggt gtttgggttg
     1141 atccgaattt acagttcacc cttgacgatt ccatttactg atattgtttt gatgcaaccc
     1201 ctcaccactt ataagtttgg caccaacgtg ttgatcgtgg ctggctatat ggtgcttgcc
     1261 atgccataca tgtaccgttc ggtggatacg ggcatgcgca ctgtggatgt agcaactttg
     1321 acagaagcgg ctcaaagcct cggcgcgagc tggttcacca tcatcacacg gatcattctc
     1381 ccgaacattc gctcggcgct ccttagtggt gccttgttaa ccttcgccat tgtagtgggc
     1441 gaagtgaccc tcgcttcctt cctcggcgtg gatgcgttcg gtccgtatct cttcctgttg
     1501 gggcagcatc gtgcatatga acctgccgcc ctttcatttg taagcttcct gttgacatgg
     1561 gcagcgatgg ggttgatcca attattcact ggcggacaag gtcagaccgc cggggcacac
     1621 taatcttttg aggagaatgg caatggcatt tctttccatt tcaaatgttt caaagcaata
     1681 cggcgatgcc acggtggtgg ataatttcaa tctcgatatc gagaagggtg aatttgtatc
     1741 ttttcttggt ccgtctggct gtggaaagac cacaacctta cgcatggtgg cagggtttga
     1801 aatcccaacc atggggaaga tcattctgga tggcgcggat attaccaata aggcacccaa
     1861 tcaacgcaat gtgggtatga tcttccaatc ctatgcccta ttcccgaata tgaccgttgc
     1921 ccagaacatt gggtttggtc ttcgagttcg caaggagccg gaagaaaccg tgaaaaagcg
     1981 tgtggacgag atgatcaacc tgatcaatct cgaaaagcat gcccacaaat atccctatca
     2041 actttcgggc gggcagcagc aacgtgtttc cttggctcgt gcattagcca accgtccgcg
     2101 tgtgcttttg ttggacgagc cgctttcagc tttggatgcg aaaatccgcg tttcattgcg
     2161 tacggagatc cgctcgattc aaaagtcact gggcattacc gccattttcg taacccatga
     2221 ccaggaagaa gcgctctcta tatctgaccg tatcgtggtt atgaatgtgg gtgtgatcga
     2281 gcaggttggt acgccctttg agatttacaa cttccctcaa acccagttcg tggcgaactt
     2341 cgttggctcc ctcaacactg ccaaagcaga agtgacagac cctgcgaatg gacttttgac
     2401 tgtggatggt gtccaatttg tgactgcctc tgatatcaaa tcccgcaaga agggggatgc
     2461 gatccgtgtt tccatccgcc cggaacgctt cagctttgcg gatgttcaaa agaaagataa
     2521 tgttctcgac tgtacgattg agaatattac cttcctcggt tctgtggtcc gaattcaggt
     2581 aaaaataggt gaatccaaat tcaatatgga taccttcaat aatccgttcc ttgaactgcc
     2641 aaagatcggt tcgaaagaac aggttacctg ttcaaaagaa gcggttctgg ttctggacga
     2701 ataaaaaaat tgtaggtcac gctcaaagcg tgacctactt ctttatagag gttttatgag
     2761 caaagctatc cttgttcttt ctgatgccct gcgttacgac gtagcgattt ccaacatggg
     2821 ctatttaggt cacatgttgg aatataaaaa agcaagcttg tacaagatcg ttggtgaact
     2881 gccaagcatg tctcgcccga tgtatgagac catccatacc ggtatgcctt ctagtgaaac
     2941 tggcattgtt gcgaattcaa ttgtgaggct ttctgaaaaa ccgaatattt ttaagtctgt
     3001 acgagaagca gggaaggtga cagccgctgc agcgtattat tggtattctg agttgtacaa
     3061 tcgcgcgcct tacaatccca ttgacgatag ggaagtggat gatgcttccc taaacattca
     3121 gcatgggcgt ttctatacca ccgacgaata cccagatatc gagctgttca acacggcggg
     3181 acatctcgtc cgcaaatatt cgccagatta tcttttgctt caccctatgg gcatggacta
     3241 tcatggcgag acctatggtt ctgatactaa ggagtatcgt aaccatgcca tctatcaaga
     3301 ttcaaaactc gcgcctcttc tcgtggaatg gcgcgagcgt ggatacacaa tttttgtaac
     3361 aggtgaccat ggcatcaatg ctgatgggg
//
LOCUS       DAOQNJ010000262         3379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19283_length_3379_cov_6.835740, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000262 DAOQNJ010000000
VERSION     DAOQNJ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19283_length_3379_cov_6.835740"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="PLG52_08305"
     CDS             <1..1251
                     /locus_tag="PLG52_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41488.1"
                     /translation="REIPIAAVVASIPRPNQAFWTLSALWAGWLWGSDAVEPYKVALR
                     RRRYDWAWSATALFAMFSHLDELLADGVPFFGLMPEPEAPFMTSALTAASAAGFKLES
                     VALRTEHDPVQVLWKSGKRSASAEIEVETARNAMRSYIAARGEPAGYLHLHTAGLIAL
                     AEVNALKQANDEFDFAMRKTQSVMEQALKDSHEFVHYSTGEGVDTGVWSLASGHAESL
                     SDKIEVGIVNYLQKNPQTIFLEVEEELNKQFTGLMTPSKGLIYAILNSYAEKDGGIWN
                     LRNEDYASARRDEMKTVFALLEEIGERLEYKTKIEEKTVTWFESGKAVRKFYVMASAL
                     IHRALEQIDEQTVIVIPGGRAALAAYKQERDPSLKERLKKHKLAKYRLLRSVLDLPIL
                     TRETFEELIASDPVEKAENQLMMF"
     gene            1293..2303
                     /locus_tag="PLG52_08310"
     CDS             1293..2303
                     /locus_tag="PLG52_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB41489.1"
                     /translation="MKKIFPFLFLALLLSSCISVEVKEADTPVPSNFVTATLAPTKAG
                     YVPPTFTETPAITQTPTFAVTIDPNCQDTAVLVRDVTVPDGTQMKPGEKFTKTWEFIN
                     SGDCPWYGYTIKFAAGDQMNAPLSAPIPDTLIKKSVQVSVDLTAPTTNGSFTGYFTLN
                     NPTGKDVPIGIEKTFWVKITVGSGSPAPQATVSSSTQLSSGSVGANCNYSQNAGYVNQ
                     IASLINQERANAGLPALTINSLLTNAAQGHAADMACNKNISHTGSDGSSAYARILSSG
                     YAPSYAEEIIYGGGGPQAAMTWWMNDQIHRDAILSAKSSEMGVGYANYSGDYIVVDFG
                     SP"
     gene            complement(2344..3213)
                     /locus_tag="PLG52_08315"
     CDS             complement(2344..3213)
                     /locus_tag="PLG52_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNB41490.1"
                     /translation="MKKYILLFALLLSACNLPGSTASTAPVTEETISEAPQTADVPQA
                     DSNPPSDIQPAAPLPAGPVHMVALGDSLTQGDGDDSGRGYPGRLLEMVNAIRPDSTLS
                     NFGQSGWSSDALINGDQGLESQLTRAVSELQDATDQGKATLALVWIGSNDLWYLYEYG
                     GDIDDAGDTSDAQRFEANIDTILSQLRGTGAQVVIALLDDQSKRPVALKGEAFVSITP
                     DEMNRMSAHVDVYNQIIRDKAAQYGALTVDFYSTDIFTNPATLYDDGNHPNQAGYDII
                     TQKWFDAISPLLK"
BASE COUNT          883 a          752 c          873 g          871 t
ORIGIN      
        1 cgtgagattc ctattgcggc ggtggtggct tctattccaa gaccaaatca ggcgttttgg
       61 actctttcag ccttatgggc aggctggttg tggggcagtg acgcggtgga gccatataaa
      121 gtagcgttgc gacgcaggcg atatgactgg gcatggagtg ccacagcttt gttcgcgatg
      181 ttcagtcact tggatgaatt gcttgcagat ggtgtgccgt tctttggttt gatgcctgag
      241 cccgaagcac cttttatgac ttcggcttta accgcggcat cggcggcggg gttcaaattg
      301 gaaagtgtcg cattgcgcac tgaacatgac cccgtgcaag tgttatggaa aagcggcaaa
      361 aggtcagcat ctgctgaaat agaagtagaa acggcacgta atgcaatgcg tagctatata
      421 gcagcacgtg gtgaaccggc ggggtatttg catttgcata ccgcagggct gatcgcgttg
      481 gcggaggtca atgcattgaa gcaagccaat gatgaatttg actttgccat gcgcaagact
      541 caaagtgtga tggaacaagc cctgaaagat agccatgaat ttgttcatta ctctacgggc
      601 gaaggcgttg atactggtgt atggagcttg gcaagcggac atgctgaatc gctttcagac
      661 aagatcgaag ttggcattgt gaactatctg caaaaaaatc cgcaaacgat ctttttagaa
      721 gtagaagaag aattgaataa gcagttcaca ggcttgatga ctccatccaa aggtttgatc
      781 tatgccatcc tgaattccta tgcagagaaa gacggcggaa tttggaattt gcgcaatgaa
      841 gattatgcgt cagctcgccg tgatgaaatg aaaacagttt tcgcattgtt ggaagaaatt
      901 ggggaacgcc tggaatacaa aactaaaatt gaagaaaaaa cagtcacttg gtttgaaagt
      961 ggaaaagctg ttcgtaaatt ttatgtaatg gcttcggcat tgatccaccg tgcgttggag
     1021 caaattgatg aacagaccgt catcgttatc cctggcgggc gggcagcgtt ggcagcctat
     1081 aaacaagaac gcgacccttc actcaaggaa cgattgaaga aacataaatt ggcaaaatat
     1141 cgtctattgc gcagtgtgtt agacctgcct attctgactc gcgaaacttt tgaagaacta
     1201 atcgccagtg acccggtgga aaaagccgaa aatcaattga tgatgttcta actcacttca
     1261 tcggtgaacg aaaatagctt tggtaaaact caatgaaaaa aatattccct tttctttttc
     1321 tcgcacttct tttatcttcc tgtatttcgg tcgaggtcaa agaagcagat acccccgtgc
     1381 cttctaattt tgtaacggca accttagctc ctacgaaggc ggggtatgtt ccgccaactt
     1441 tcaccgagac tccggcgatc acacaaacgc ctacctttgc agtcaccatc gacccgaact
     1501 gtcaggatac cgccgtgctc gtccgcgatg tgactgttcc tgatggaacg cagatgaagc
     1561 ccggtgaaaa attcaccaaa acttgggaat tcatcaacag cggcgattgc ccgtggtatg
     1621 gatacaccat caaatttgcg gcaggggatc aaatgaatgc gcctctttcc gcaccgatac
     1681 cggacacgct tatcaagaaa tctgtccaag tttcagtgga tctgaccgcc ccaaccacga
     1741 acggatcctt tacaggttac ttcacgctca acaacccaac gggtaaagac gtgccaattg
     1801 gtattgaaaa aacgttttgg gtgaagatca ctgttggcag tggaagccca gctccacagg
     1861 cgacggtttc tagtagcacg caattgtctt caggtagtgt aggcgcaaac tgcaattaca
     1921 gtcaaaatgc tggttatgtt aatcaaatcg catcgttgat caatcaagaa cgggcaaatg
     1981 ccggtttgcc tgctttgacg attaattcct tgctgacgaa tgcagcacaa ggtcacgccg
     2041 cagatatggc atgtaataaa aatattagcc atacgggttc agatggctct tcagcatatg
     2101 ctcgaattct ttcctcagga tatgcgccat cgtatgcgga agaaattatc tacggcggag
     2161 gtggtccgca ggcggcgatg acctggtgga tgaacgatca aatccatcgc gatgcaattc
     2221 ttagtgcaaa atcgtcagaa atgggcgttg ggtatgccaa ttattcaggc gattacattg
     2281 tggtagattt cggcagccca taaacaaaaa gtctcttgca aacgcaagag actttttgtt
     2341 tctctatttc agcagaggcg atatggcgtc aaaccatttt tgggtgatga tatcgtaccc
     2401 agcttggttg ggatgattgc catcgtcgta gagcgtagca gggttggtaa aaatatcggt
     2461 cgagtagaag tccacggtca gtgcgccata ttgggcagct ttatcgcgaa tgatctggtt
     2521 gtagacatcc acatgggcag acatgcggtt catttcatcc ggcgtgatgg aaacgaaagc
     2581 ttcgcctttc agtgccaccg ggcgtttgga ttggtcgtct aacaaggcga taaccacttg
     2641 ggcgccggtt ccacgcagtt ggctaaggat ggtatcgata ttcgcttcga aacgttgcgc
     2701 atccgaagtg tcgccggcat cgtcaatgtc accaccgtat tcgtacaaat accaaaggtc
     2761 gttgctgccg atccatacca atgccagagt cgctttgcct tggtcagttg catcttgcaa
     2821 ttcactcacc gcacgcgtaa gctgactttc caatccctga tcaccgttga tgagcgcatc
     2881 agaactccag ccggactgac cgaaatttga caaagtcgaa tctgggcgga tggcattgac
     2941 catctctaac aggcgaccgg gatatccgcg ccctgagtcg tctccgtcac cttgggtaag
     3001 gctatcgccg agggcaacca tgtgaacagg tccggcagga agcggggcgg caggttgaat
     3061 atcactgggt gggttactgt ctgcttgagg cacatccgct gtttgaggag cttcgcttat
     3121 ggtttcttca gtgactggtg ctgtggaagc agtagacccc ggaagattgc aggcggataa
     3181 gagtaatgcg aaaagcaaga tgtatttttt catggtgttt tctcagtttg tatggtttga
     3241 ctgtaggtgt ttaattttcg gcccatttct gtaggaacgc cataaacact tcaggaacat
     3301 acgatgagct gccatcttcg agttcggctg tgttttgtga gtggattaat tccccattgc
     3361 tatccaaaat gtagatatg
//
LOCUS       DAOQNJ010000263         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19407_length_3367_cov_10.145833, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000263 DAOQNJ010000000
VERSION     DAOQNJ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19407_length_3367_cov_10.145833"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1040
                     /locus_tag="PLG52_08320"
     CDS             129..1040
                     /locus_tag="PLG52_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNB41491.1"
                     /translation="MKKLLTLASLLAVASLVLAACGGGGGSSASDLLGAIQERGYILI
                     STDPNYEPQSFLNTEGSRPADTKCPSDALTTAEMQGFDVDVATAIGDSLGVETCFATP
                     SWDAITAGNWADKWDMSVGSMTVTTARQQILDFSVPYYYTPAVVAVAADAGIDSLEGL
                     SGQSVCAGAATTYEAWLNNDTEALGLPASSIYAQVPADVTVVPLETDQECAQAIASGR
                     KDFVAYVTSETVVDANLAAGMPVVKLDGAVYSEDLAAAFDKSSSLDTASLRAKVDELF
                     KAMHEDGRLSELSNKWFEVDLTAAPNQ"
     gene            1118..1966
                     /locus_tag="PLG52_08325"
     CDS             1118..1966
                     /locus_tag="PLG52_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNB41492.1"
                     /translation="MASTTNQEQAPLSQAELLYQAQLRKERLFRTHVGVSWAVLFFIL
                     IFLFSGQTIQLGSFKFDTIELDSSFILKNLSFIAQGLGQTLLISVLSILFAMILALLA
                     ALGRLSTFPPIYAISTFYVSLIRGTPLYLQIFFFFLALPQLGIILSGIVAGVMALGLN
                     YGAYMSETFRAGLSSVGKGQREAAMALGMTPGQTMRRVILPQALRFAIPPMGNDFISM
                     TKDSALVSATGFVHELMWRATKVGRAQFNNLEALIMAAMFYWILTLILTYFQGKLEAR
                     LAKGDR"
     gene            1983..2738
                     /locus_tag="PLG52_08330"
     CDS             1983..2738
                     /locus_tag="PLG52_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNB41493.1"
                     /translation="MVKVTNLDKYFGRLHVLKDISLEVPERQVLCLIGRSGSGKSTLL
                     RCLNFLEDPSRGIIEVDGIKVDAEVKNKERRQLIHDVRLKTGMVFQEFNLFPHMTVLE
                     NVIEGPVTVKGMDKKVATELAEQNLDSVGMLFKKDEYPLRLSGGQKQRVAIARALTMQ
                     PRVMLFDEPTSALDPELIGEVLNVMKKVAYEGMTMIVVTHEMGFARDVADRVIVMSEG
                     QLIEDATPEALFTSPKDPRTKALIDRYRSDDQQ"
     gene            2754..3122
                     /locus_tag="PLG52_08335"
     CDS             2754..3122
                     /locus_tag="PLG52_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB41494.1"
                     /translation="MATPGFKITSEEIQHNDKSVTVFHLSGWVNVQSEEKVFSTAETA
                     YTNGSRYLVINLEDVQMLTSAGIRLLQRIYKLYRSTETIGLKICSAPSQVYHALALTG
                     LLQTVPMYENLQAALSAYDL"
     gene            3126..>3367
                     /locus_tag="PLG52_08340"
     CDS             3126..>3367
                     /locus_tag="PLG52_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HNB41495.1"
                     /translation="MTIAITRHISSRFNECEITHIERTPINLEIARSQHEAYVRALAE
                     LGCQVIELPEETELPDSVFVEDTAFILPEVAVITRPG"
BASE COUNT          828 a          893 c          754 g          892 t
ORIGIN      
        1 atgtttttta ataactaaaa tccttgcaca attgaaaaaa atagtctatg cttaacctcg
       61 cggagtggag agcattccgt ggcagcgaaa tcgctgccaa taatttttat ttctctcgga
      121 ggagaaaaat gaagaaactt ttgacccttg caagccttct cgctgtagca tcgctcgttc
      181 ttgctgcatg cggaggcgga ggcggttctt ccgcttcaga cctacttgga gccattcagg
      241 aacgcggata catcctcatt tctactgacc caaactatga gcctcagtct ttcttgaaca
      301 ccgaaggctc ccgccctgct gacaccaagt gccccagcga cgccctcacc acagccgaaa
      361 tgcagggctt cgatgtagat gttgccaccg caattggtga ctcactcggt gtagaaacct
      421 gtttcgctac tccctcctgg gatgcgatca ctgccggtaa ctgggcagac aaatgggata
      481 tgagcgtggg ctccatgacc gtcaccaccg ctcgccaaca gatcctcgat ttcagcgtgc
      541 cctactacta cacccccgcg gttgtggctg ttgctgctga tgctggcatc gactctctcg
      601 aaggtctttc cggtcagtcc gtttgtgctg gcgccgccac cacctacgaa gcctggctca
      661 acaacgatac agaagcgctt ggcttgcccg ccagctccat ctacgctcag gttcctgctg
      721 atgtcaccgt ggttcctctc gaaaccgatc aggaatgcgc ccaagcgatt gcctctggtc
      781 gtaaggactt cgttgcctac gtgacctctg aaaccgttgt ggatgcgaat ctcgccgcag
      841 gcatgcctgt cgtcaagtta gacggcgctg tctactctga agacctcgct gccgctttcg
      901 acaagtcctc ttcccttgac accgcttcct tgcgcgccaa agtggacgaa ctcttcaaag
      961 ccatgcacga agatggtcgc ctctccgaac tctccaacaa gtggttcgaa gttgacttga
     1021 ccgctgcacc caaccagtaa gaaaacgaaa taaaaaggga agcggatcat ctccgcttct
     1081 ctttttatta ttccacttca cctctcggag gtaaaagatg gcttccacca ccaaccagga
     1141 acaagctccg ctttcacaag ccgagttgct ttatcaggca caacttcgca aggaacgttt
     1201 gtttcgcacg catgtcggcg tttcctgggc tgttctgttc tttatcttga tctttctttt
     1261 cagtgggcaa acaattcaac ttggttcttt taagttcgac actatcgaac tagacagcag
     1321 ttttattcta aaaaatctat ctttcattgc acaagggttg gggcaaactc ttttgatctc
     1381 cgtcctctct attttgtttg caatgatttt agctcttttg gcagcccttg gcaggctttc
     1441 caccttccct cctatttatg ctattagtac gttctacgta tcgctgatcc gtggaacgcc
     1501 gctttatctg caaatatttt tcttcttcct tgccttgcca caacttggca ttattctttc
     1561 cggtatcgtc gcaggggtga tggcattagg tctgaattat ggtgcctaca tgtctgaaac
     1621 attccgtgcc ggcttaagct cggtaggcaa agggcaaaga gaagctgcca tggcgctggg
     1681 catgactccc ggtcagacca tgaggcgtgt gatcctaccg caggctttgc gttttgccat
     1741 tccacctatg ggcaacgact ttatctccat gacgaaagac tcggctctcg tctcggcgac
     1801 aggtttcgtc cacgaattga tgtggcgcgc caccaaagta ggacgtgctc aattcaacaa
     1861 ccttgaagcg ctcattatgg cagccatgtt ctattggatc cttaccctca tcctcaccta
     1921 cttccaaggc aaattggaag cccgccttgc aaagggagac cgataacatg tccacccttc
     1981 caattgtaaa agttacaaac ctcgataaat atttcgggcg tttgcatgta ttgaaagata
     2041 ttagccttga agtgcctgaa cgtcaggttc tatgccttat cggacgtagc ggttcaggga
     2101 aaagtacgct tctgcgttgc cttaactttc tcgaagatcc ctctcgtgga atcattgaag
     2161 tagatggcat caaggtagat gctgaagtca agaacaaaga acgtcgccaa cttattcacg
     2221 atgtgcgttt gaagaccggc atggtgtttc aagaattcaa cctcttccca cacatgaccg
     2281 tattagagaa cgtgatcgaa ggtcctgtaa ctgtaaaggg catggataaa aaagtcgcca
     2341 ctgaactcgc agaacaaaac ctggatagtg tgggcatgct tttcaagaaa gacgagtatc
     2401 cgctgcgcct atcgggcggg caaaaacagc gcgttgccat tgcgcgcgcc ctcaccatgc
     2461 aaccgcgtgt gatgctcttc gatgaaccaa cctctgcatt agaccctgaa ctcattggcg
     2521 aagtattaaa tgtgatgaag aaagtggctt acgaaggaat gaccatgatc gttgtcacac
     2581 acgaaatggg ctttgcccga gatgtggcag atcgtgtcat tgtgatgtct gaaggtcagt
     2641 tgatcgaaga tgccacccca gaagcgttgt ttaccagtcc taaggaccct cgcaccaaag
     2701 ccctcatcga ccggtatcgt tccgacgacc aacaataata aggcatcttt ttcatggcaa
     2761 ctccgggttt caagataaca agcgaagaaa ttcaacataa cgacaaatct gttactgtat
     2821 tccacttgtc tggctgggtc aacgtacaaa gcgaagaaaa agttttttct acagcagaaa
     2881 cagcctacac aaatggctct cgctatcttg ttatcaatct ggaagatgta cagatgctca
     2941 ccagcgctgg gatacgcctt ctacaaagaa tctataaact atatcgttct accgaaacaa
     3001 tcgggttgaa gatttgcagc gccccatctc aggtctatca tgcgttggct ctaactggct
     3061 tgctacaaac cgtgcctatg tatgaaaacc ttcaagccgc gctttctgca tacgatctct
     3121 aaatcatgac cattgctatt acccgccata ttagttctcg tttcaacgaa tgtgaaataa
     3181 ctcatattga acgcacaccc ataaacttgg aaattgcacg ttcgcagcat gaggcgtatg
     3241 ttcgtgcact agctgagttg ggctgtcagg tcattgagct tcccgaagag acagaacttc
     3301 ctgattcagt cttcgttgaa gatacagctt ttattctgcc tgaagtcgct gtcattacca
     3361 gacccgg
//
LOCUS       DAOQNJ010000264         3364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19444_length_3364_cov_8.199456, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000264 DAOQNJ010000000
VERSION     DAOQNJ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19444_length_3364_cov_8.199456"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1155
                     /locus_tag="PLG52_08345"
     CDS             253..1155
                     /locus_tag="PLG52_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNB41496.1"
                     /translation="MSKNIFSELSEAMANAAEKAGKYTVLVDARRRMPASGIAIDKET
                     ILTAGHVVEREEDIKILFADGKEVTARLIGRDPGTDLAVLKLDSAVATPAELAKTPAR
                     AGQFVLAVGRPSANGIESSFGTVNKISGPVRTGRGGMLEKYIKTDVVSYPGFSGGPLI
                     NGDGEVYGINTSGFGMGGEAITIPADLAWKIAETLTKHGKIKRGFIGVRSQTVNLPAD
                     AQAQLNREQETGLLLVGIEKDSPAEKGGLIIGDILVGVAGVAVEHHDELFTRLSGDVV
                     GKPTAMDVLRGGKLQSVSVVVGER"
     gene            1215..1820
                     /locus_tag="PLG52_08350"
     CDS             1215..1820
                     /locus_tag="PLG52_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB41497.1"
                     /translation="MIRVTIVSPNHALRVGLREMLANQAGIRVVGEATRLSDVNERET
                     EVAVLASVSSVRSVDQKDFCILFLTDDIESVRPLLSSGMSAWGVLSSEANADELAAGI
                     AAVAEGLWVGAPGLVEGLMRLPKGGEGMGEESLPEPLTAREKEVLQHMAEGLANKQIA
                     LALRISEHTVKFHLSALYAKLGAASRTEAVKRGIELGLISL"
     gene            1893..>3364
                     /locus_tag="PLG52_08355"
     CDS             1893..>3364
                     /locus_tag="PLG52_08355"
                     /inference="COORDINATES: protein motif:HMM:NF039926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNB41498.1"
                     /translation="MPNEFHYKWEWDLASSPEALWPLASDTNRFNRDTGLPPMQLLGI
                     QNRVKQVKFKVPIVNIVWDEEPFEWTYPYRFGILRRYRTGPLQEMRVDCRLERRDAGT
                     HLIYEVWVKARNVLGDIAIPLGIGIYSAKRFEDAFKLYDRIASTSNTLPGTTTKQNLS
                     SSGHSRYRLLRERLIAQNVDQTTLDRLYEYFQHADDLSIQRMRPYALADGWNLPRRAV
                     LEAFLFATREGILDMYWDLLCPECRGVAEDHKRLGDLHAHSHCNTCQIDFNVNFDQNV
                     EVIFRPNPSIRQVDTSIEFCVGSPQRQPHIVFSLIVPPREQLPFNTMLKAGRYRLTAS
                     AVDGSQILTAASDGPATMDFHADKLGWNSDFLKIGLNPNVRLFNDSDFTQTFQMERVV
                     WSDQAATAADVTTLQVFRDLFSNEVLRVDEEISVGSMTLMFTDLRNSTKMYREIGDAP
                     AFGRVREHFEILEQAVAKEDGAIVKTMGDAVMAAFRKPVSA"
BASE COUNT          833 a          776 c          895 g          860 t
ORIGIN      
        1 ccacgcagaa ggatatcatg gcgggcagac cttccgagct taatcagcaa actggtgcgg
       61 tcattcgtat gggcaaggca gcgggtgtgc ctaccccaac ccatgaaaaa atttttgcag
      121 ccttgttgcc gttcgaacaa aaggctcgtg cgcaataatc cccactagcc gaatgtctag
      181 tatcgcccca gtcaattcac cgatacactc caaacacatg ctcgctagaa tgagcataaa
      241 ggagtaacga aaatgagcaa aaatattttt agtgaacttt ctgaagccat ggcaaatgct
      301 gccgagaagg ctggcaaata tacagtcttg gtcgatgccc gccgccgcat gcctgccagt
      361 ggaattgcaa ttgataaaga aacgattctg actgcaggtc atgtagtaga acgcgaagaa
      421 gatatcaaga ttttattcgc ggatggaaaa gaagtcactg cacgcctcat cggtcgggac
      481 ccgggaacgg acctcgccgt cttgaaatta gactccgctg tagccactcc tgccgaactc
      541 gcaaagacgc cagcacgtgc ggggcagttt gtcctcgctg tggggcgccc ttccgcgaat
      601 ggaattgaat ccagctttgg caccgtgaac aaaattagcg gacccgtccg caccgggcgc
      661 ggtggcatgt tggagaaata catcaagacc gatgtggtct cttaccccgg tttctctggc
      721 ggaccactca tcaacggcga tggcgaagtg tatggcatca acacctctgg ctttggcatg
      781 ggcggagaag caattaccat ccctgctgat ttggcatgga agattgcaga gacccttacc
      841 aaacatggca agattaaacg cggctttatc ggtgttcgca gtcaaaccgt aaatctccct
      901 gccgatgcgc aagcacaact taaccgtgag caagaaacag gtttgctgtt ggttggcatt
      961 gaaaaagaca gtcccgcaga aaaaggcggg ctgatcatag gcgatattct tgtcggtgtc
     1021 gctggcgttg ccgtggagca ccacgacgaa ttattcaccc gtttgagcgg cgatgtagtt
     1081 ggcaagccta ctgccatgga tgttcttcgt ggtggtaagt tgcaatcggt tagtgttgtt
     1141 gtaggcgaaa gataaaaata taaagagcac agagcataaa actctgtgct ctttatcgca
     1201 agaaaggctt tattttgatc cgtgtaacca tcgtctcacc gaatcatgcc ttgagggttg
     1261 ggttgcgtga aatgcttgcc aaccaagcgg gcattcgtgt ggtgggggag gcgactcgtc
     1321 tttcagatgt caatgaacgc gaaaccgagg tggctgtgct tgcctcggtt tcttctgtac
     1381 gtagtgtaga tcaaaaagat ttttgcattc tttttctgac agatgatatt gaatccgtac
     1441 gacctttatt atcatcgggg atgagtgcgt ggggggtctt atcttctgaa gccaatgcgg
     1501 atgaattagc agcgggaatc gctgcagtgg cagaaggcct ttgggtgggt gcacccggct
     1561 tggtcgaggg cttgatgcgg ttgccaaagg gcggcgaagg catgggagaa gagtctttac
     1621 cagagcctct cacggcacgc gagaaggaag tcctccagca tatggcggag ggacttgcca
     1681 acaagcaaat tgcgctggcg ttgcgaatta gcgaacatac ggttaagttt catctttcgg
     1741 cgttgtatgc caaattgggg gcggcaagcc gcaccgaagc tgtgaagcgc ggcattgagt
     1801 tggggttgat ttcgttgtaa tttcttattt gtctgtaatg ctgttgacag gtttggcagg
     1861 taaaataatt agactatata ggtctatata tcatgccaaa cgaatttcac tacaaatggg
     1921 aatgggactt ggcgtcttcg ccggaagcat tgtggccgct tgcgtcagat acaaatcgct
     1981 tcaatcgtga taccggcttg ccgcccatgc agttgttggg gattcaaaat cgggtcaagc
     2041 aggttaagtt caaggtgccc attgtaaata ttgtatggga tgaagaacct ttcgaatgga
     2101 cgtacccgta ccgctttgga attcttcgtc gctatcgaac cggtccgctg caagagatgc
     2161 gtgtcgattg ccgcttggag cgccgagatg ctggcacaca tttgatctac gaagtgtggg
     2221 tgaaagcccg caatgtcttg ggggatatcg ctattccgct gggaatcggg atctatagcg
     2281 cgaaacgatt tgaagatgca ttcaaattgt atgaccgcat cgcttcaact tctaacacgc
     2341 tgcctggtac taccaccaaa cagaatctca gctcaagcgg acattcccgc tatcgtctat
     2401 tgcgcgagcg gctaattgct caaaatgtag atcaaaccac cctcgaccgt ttgtatgaat
     2461 attttcaaca tgctgatgac ctatccattc agcgtatgag accctatgcc ttggcagatg
     2521 gttggaatct gcctcgccgt gctgtgttag aggcatttct ttttgccact cgcgaaggga
     2581 ttttagatat gtattgggat ttgctatgcc cagagtgccg aggcgtggca gaagaccata
     2641 aacgtttggg cgatttgcat gcacattcgc attgcaatac ttgtcagatc gatttcaacg
     2701 tgaactttga ccagaatgta gaagtgatct tccgcccgaa cccatctatt cggcaagtag
     2761 atacatcgat tgagttttgt gtaggcagcc cacaacgcca acctcatatt gttttctcgt
     2821 tgatcgtccc gccgcgtgaa caattgcctt ttaataccat gttgaaggcg ggtcgttatc
     2881 gcttaactgc ttcggcagta gatggatcac agatattaac cgctgccagt gatggtcccg
     2941 ctacgatgga tttccatgcc gataagttag gttggaacag tgacttctta aagatagggc
     3001 tgaacccaaa tgttcgccta ttcaatgatt cagactttac ccaaaccttc caaatggaac
     3061 gagttgtttg gtcagaccag gctgccaccg cagcagatgt gacgacctta caagtattcc
     3121 gtgacctatt ctctaatgaa gtcttgcgtg tggatgaaga gatttcagtc ggttcgatga
     3181 cgttgatgtt taccgatctg cgcaattcca ctaagatgta tcgtgagatc ggtgatgccc
     3241 ctgcctttgg gcgtgtccgc gagcattttg agattttgga acaagccgtg gcaaaagaag
     3301 atggcgccat cgtcaaaacc atgggcgatg cggtcatggc agcctttcgt aaaccggttt
     3361 cggc
//
LOCUS       DAOQNJ010000265         3364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19445_length_3364_cov_6.187066, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000265 DAOQNJ010000000
VERSION     DAOQNJ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19445_length_3364_cov_6.187066"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1278
                     /locus_tag="PLG52_08360"
     CDS             73..1278
                     /locus_tag="PLG52_08360"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB41499.1"
                     /translation="MESNHTYKVRSRILNRETFIRAQFTGAQKGAEMAWIKVIVRPVE
                     LRGEVHLQFSFFDEKKDISKNYTFEEASARIDEVLALPFRNIFIENTTGTLQVNISKK
                     GKALVNEHKSAPTALPVVDLSHNREKSRLLSAENAKPFLEAVGILTKDGRIKADMQRK
                     YTQINEFLRLVDESDALESFAGKPVHVVDFGCGNAYLTFAVYYYLHEILKLDAHVTGV
                     DIKADLIERHQEKARKLGWSQLEFKVGTIGDFVPASTPDIVVALHACDTATDDALANG
                     IRWESKVIVCAPCCQHELQVQMAQVPAPTALASVLSHGILFERMGDILTDTFRATLLR
                     IMGYRTDVTQFVPIEHTAKNLMIRSVKVSPAGKNPRWLEEYKNLKSFWQVTPYLEKLL
                     GENYAQYVE"
     gene            1355..2167
                     /locus_tag="PLG52_08365"
     CDS             1355..2167
                     /locus_tag="PLG52_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41500.1"
                     /translation="MLTKTSRVLTYLLAALYAVIGGVLFFRPEQQAPIFAWNVTGFMT
                     MTIGAWCLGNAWLAFFTARRWNWQLVYPALSYLWSFGLLEGMIVVSFREKLNLDYPVA
                     WFYVSVISLNAIAAAFGIVDWLRVRPAGSPSEPMTGLMRGVATAFVLFVGFLGVYGLS
                     VQAGAPATTGEIFPEAMTTLTLRSFGAFFLALTIGMLPLLFEKNRAPFLNYSFLAFGL
                     VIIITIAAFAYFPLFNFGEHPFGSLYFLAYFVAAGVSIFFFRKFGTGATTKA"
     gene            complement(2168..2950)
                     /locus_tag="PLG52_08370"
     CDS             complement(2168..2950)
                     /locus_tag="PLG52_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41501.1"
                     /translation="MPPSTIGGINLLSEPEKRAIYTKYIPQALLDKYNLPPLTSAAGY
                     NLLQFRFANGSTDVEMRLYHKVDFPDPILYAHLTDTMNAQIHVLLYVLNDPDSPRYDV
                     DKMPDGTPTKFGTLRRNIEAETKACEAGLAPGQVRRGLRLLGGAIEAFEGFVTALGHD
                     MYFVEPLYYHNAVIFERYGFSYQMGKRLMESINTGFQPGGDLHSQLDGSNAFRKTEAA
                     ESIRKRSWAIHDGILGEPFTNVTMYKRVGHSAGVDTTKGCKW"
     gene            complement(3009..>3364)
                     /gene="smpB"
                     /locus_tag="PLG52_08375"
     CDS             complement(3009..>3364)
                     /gene="smpB"
                     /locus_tag="PLG52_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNB41502.1"
                     /translation="SIRAGQISIQEAYVDVENGQEAWLLEAHIAPYEQAGKYFNHEPK
                     RKRRLLMHKKEIRELWNNVRIKGMTIVPTKVYLKDGRAKVEIALAKGKKAYDKRATIA
                     KRDEARDKERAMRVR"
BASE COUNT          739 a          873 c          841 g          911 t
ORIGIN      
        1 aataaaaata cttaagaatg aatttcagta atattttttt tcttttgatt atcattatcg
       61 tataatctca ttatggaatc aaaccacacc tacaaagtcc gttcgcggat actcaaccgc
      121 gagaccttca tccgtgccca atttaccggt gctcaaaaag gggcggaaat ggcgtggatc
      181 aaggtcatcg tccgcccggt ggagttgcgg ggtgaggttc acttgcagtt ttcctttttc
      241 gatgagaaaa aggatatttc caagaattac acgtttgaag aagcatccgc ccgaatagat
      301 gaagttctgg cgttgccttt ccgcaatatt tttatagaaa atacgacggg caccttgcag
      361 gtcaacatct cgaagaaggg caaagccttg gtcaacgagc acaagtccgc gcccacggcg
      421 ctgcccgtgg tggatttatc ccataaccgc gagaagagcc gcttgctttc ggcagagaac
      481 gccaaaccgt ttttagaggc ggtcggcatc ctcaccaaag acggcaggat caaagccgac
      541 atgcagcgca agtacaccca gatcaacgag tttctgcgct tggtcgatga aagcgacgca
      601 ctcgaaagct tcgcgggcaa acccgtccat gtggtggatt tcggctgtgg caatgcctac
      661 ctaaccttcg cggtttatta ttacctgcat gaaattctga aactcgatgc acacgtcacc
      721 ggcgtggata tcaaagcgga cttgatcgaa cgccatcagg aaaaagccag aaagctcggc
      781 tggagtcaac tagagttcaa ggtcggcacc atcggggact ttgtgcctgc ctctacaccc
      841 gatatcgtgg ttgccctgca tgcttgtgat accgccaccg acgacgcgct tgctaatggc
      901 atccgctggg agagcaaggt tattgtctgt gcgccatgtt gtcagcatga gttacaggtg
      961 caaatggcac aagtccctgc accgacggcg cttgcttcgg tgctcagtca cggcatcttg
     1021 ttcgagcgta tgggtgatat cctcaccgat accttccgtg ctacgctatt gcgcatcatg
     1081 ggctatcgca ccgatgtcac tcaatttgtg cccattgaac acaccgccaa gaatctaatg
     1141 atccgctcgg tgaaagtttc gcccgcgggc aagaatccgc gctggctgga agaatacaaa
     1201 aacctcaaaa gcttctggca agtcacaccg tatttggaga agttattggg cgaaaattat
     1261 gctcagtatg tagaatgatc cttttggggt gaaaactcaa gcaccaacgt agtgtttata
     1321 tcttttgttc caaactttaa tagaggagag aattatgctt accaaaacat ctcgtgtgtt
     1381 aacctatctg ctcgccgcgc tctacgccgt cattggcgga gtcttgtttt ttcgccccga
     1441 acaacaagcg ccgatctttg cctggaatgt gaccggcttc atgaccatga ccatcggcgc
     1501 ctggtgcttg ggcaatgcct ggctggcgtt cttcacggct cgccgctgga actggcaact
     1561 ggtctaccca gccttgtctt atctctggtc ctttggcttg ttggaaggca tgatcgttgt
     1621 gtctttccgt gagaagctga atttagatta ccccgtggct tggttctatg tatctgtgat
     1681 tagcctcaac gccatcgccg ccgccttcgg catcgtagac tggttacgcg tccgcccggc
     1741 gggcagcccc tccgagccga tgaccggtct catgcgcggc gttgcaacag cattcgtcct
     1801 ttttgtaggc tttctcggcg tgtatgggct gagcgttcaa gcgggtgcgc ctgccacaac
     1861 tggcgaaatc ttccccgaag ctatgaccac cctcacattg cgcagctttg gcgctttctt
     1921 ccttgccctc accataggca tgttgccctt gttgttcgaa aagaatcgcg ctccgttttt
     1981 aaactacagc tttctggcat ttggcttggt cattattatt accattgcgg cgttcgcata
     2041 tttcccgctc tttaactttg gcgaacaccc ctttggtagc ctttattttc tggcgtactt
     2101 tgtggcagcc ggagtttcta ttttcttctt ccgcaaattt ggtacaggcg ctactacaaa
     2161 agcatagtta ccacttacaa cctttggttg tgtccacacc cgcactgtga cctacacgtt
     2221 tgtacatggt cacattggta aaaggctcgc cgagaatgcc gtcgtgaatc gcccaagagc
     2281 gtttgcgaat gctctcggcg gcttctgttt tccggaaggc gttcgagccg tctagttgag
     2341 aatgaaggtc gccaccgggt tgaaaaccgg tgttgatgct ttccatcaag cgtttgccca
     2401 tctgatacga aaaaccataa cgctcgaaga taactgcatt gtgataatac aacggctcga
     2461 cgaagtacat gtcgtgacct agcgctgtga cgaagccctc gaacgcttca attgcgccgc
     2521 ccaataacct cagcccgcgt cgtacctgac ccggtgccag cccagcctca caagcctttg
     2581 tctccgcctc gatattgcgc ctcaacgtgc cgaacttggt gggtgtgcca tcgggcatct
     2641 tgtccacgtc atagcgcgga gagtctgggt cgttgagcac atacaacaac acatgaattt
     2701 gagcgttcat ggtgtcggtt aaatgtgcat ataagattgg gtcggggaag tctactttgt
     2761 gatacaggcg catttccaca tcggttgagc cgttcgcaaa gcggaattgc agcaggttgt
     2821 atcctgccgc ggaggtcaat ggcggcaggt tgtatttgtc taatagagct tgcggaatgt
     2881 atttggtgta gatcgcccgc ttttcgggtt cagataataa attgattccg ccaatggttg
     2941 agggaggcat agcttcttct ttcttctttc acctctcctc cctatataaa gaggaaagat
     3001 gaaagaggtc atcttacccg catggcacgt tctttatcac gcgcttcatc tcgtttagca
     3061 atggtggcgc gtttgtcgta ggctttcttg ccttttgcca gtgcgatctc gaccttggca
     3121 cgcccgtctt tgagatagac cttggtcggg acaatggtca tgcctttgat gcggacgttg
     3181 ttccaaagct cgcggatctc ttttttgtgc ataagtaaac ggcgtttgcg cttcggttcg
     3241 tggttgaagt atttacccgc ttgttcataa ggcgcaatgt gtgcttcgag cagccaggct
     3301 tcctgtccat tttccacgtc cacataggct tcttgaatgc tgatctgccc ggcacgaatg
     3361 gatt
//
LOCUS       DAOQNJ010000266         3363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19458_length_3363_cov_10.386941, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000266 DAOQNJ010000000
VERSION     DAOQNJ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19458_length_3363_cov_10.386941"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1351)
                     /locus_tag="PLG52_08380"
     CDS             complement(92..1351)
                     /locus_tag="PLG52_08380"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB41503.1"
                     /translation="MNFNNFNLDPRLKQGIMKAGYETPTPIQEAAIPAAIRGRDIIGT
                     AQTGTGKTAAFVLPILNKLLNGQRNTPRALIVTPTRELAEQINDVIKTLSAGTKLKSA
                     TIYGGVGANPQIQALRNGAEILVACPGRLLDLIAQGHAKMANIEILVLDEADRMFDMG
                     FLPDVRRIVKAVPEKRQTMMFSATFPPDVELLAKQALREPQKIAMGIVKPAHTVEHAL
                     YPVPQHLKTKLLIELLKRTDTNSVIIFTRTKHRAHRVSEQIKKSGFKATSLHGDRTQG
                     QRQAAIKGFKSGTHDIMVATDIAARGLDIETVSHVINFDMPDTADAYIHRIGRTGRAQ
                     RTGDAFTLVTDEDKDMIRTLEKIMGQPLKRETLEDFDYTAAAPPRTDSGGRGRGAPRD
                     GGDGRRPPKPAPTPKSYGRNRLAPRKK"
     gene            complement(1422..2288)
                     /locus_tag="PLG52_08385"
     CDS             complement(1422..2288)
                     /locus_tag="PLG52_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB41504.1"
                     /translation="MRRLTGIILIAISAASFGTLAIFGRYAYAENIDTFTLLFLRFGF
                     SASFMTMMLILRKEPFPRGRILAQLIGMGGIGYAGQSFLYLTAIKYASAGLVALLLYL
                     YPFFVAILSMIFLQEKLTRVKAFALALALCGTALTVGPVSGQPIGAIMAIVAALGYSI
                     YIIVGANVMKHVSPFQSSTVIFASASVVYGLLTFTNGIHLPQTNFGWLLIAGIVVVST
                     IIPVTTFLAGLERIGPTNAAMLSTIEPIITVFLATVLFGDKLAPIVLAGGALILIAVV
                     ILTRSEMRSIET"
     gene            complement(2375..2968)
                     /locus_tag="PLG52_08390"
     CDS             complement(2375..2968)
                     /locus_tag="PLG52_08390"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNB41505.1"
                     /translation="MTVYSYQINGMSVQTGDIICTMNGKPDILPGEFWRLVGRLVPGD
                     VDHVAIYLGPDGRCAEAGAKGVITFDVPNGVWNTERMARQRGLLFDTFYGVVSPLDVL
                     GYMEEDEFHLRKAVAQYILAQVGKPYNLNFFNSETEEAFYCSQLAYKAYQTIGINLNT
                     GLAMEQLPGTNQIVYPQEIWNGFSHHKINLFRAAVSA"
     gene            complement(3002..>3363)
                     /locus_tag="PLG52_08395"
     CDS             complement(3002..>3363)
                     /locus_tag="PLG52_08395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB41506.1"
                     /translation="SLHGALNNQPLVDKLKQAHVLVVPSSYEGFGIVYLEGMGFGLPA
                     IGTTLGAASEVIDDAVTGFLIQPGDAHRLAEKLQLLSENRDLLLKMSLAARSRYQRQP
                     KWNQTANQIRDFLLSFI"
BASE COUNT          838 a          802 c          892 g          831 t
ORIGIN      
        1 ggaccgaagg gtcctcaggc tagcaacgtt accatcttga gctagtagac aaaaagagcc
       61 ggatgcgaaa gcatccggct cttttttgat tctacttctt cctcggcgcc aatcgatttc
      121 tgccataact cttcggcgtt ggcgcaggct tgggcgggcg acgtccatca ccgccatcac
      181 gcggagcacc tctgcctctt ccaccagaat cagtgcgtgg cggggcggca gctgtgtaat
      241 caaaatcttc aagcgtctca cgcttaaggg gttgccccat aattttttca agcgtacgga
      301 tcatatcctt gtcttcatcg gtcaccaaag tgaaggcatc gccggttcgt tgcgcacgac
      361 cggtgcgacc aatgcggtgg atgtaggcat cggcggtgtc gggcatgtca aagttgatga
      421 catgtgagac cgtttcaatg tccaaaccgc gggctgcaat atcagttgca accatgatgt
      481 cgtgggttcc gcttttgaag cctttgatgg cagcctgccg ttgcccttga gttcggtcac
      541 cgtgtaggct ggtggctttg aagccagatt tcttgatttg ctctgataca cgatgagcac
      601 gatgtttggt gcgtgtaaag ataatcacag aattcgtgtc ggtacgcttg agtaattcga
      661 tcagcaattt tgttttcaaa tgttgaggca cggggtaaag cgcatgttcc accgtatgcg
      721 ccggtttgac aatgcccatc gcgatcttct gtggttctct taaagcttgc tttgccagca
      781 gttctacatc aggcgggaag gtggcagaga acatcatcgt ctgtcgtttt tcaggcaccg
      841 ctttcacaat acggcgcaca tcaggcagaa agcccatgtc gaacatgcgg tcggcttcat
      901 cgagcacgag aatttcaata tttgccatct tggcatggcc ttgtgcgatg aggtccagca
      961 gacgccccgg gcaagccact agaatttcag ccccgttgcg caaggcttgg atttgtgggt
     1021 tcgctccgac tccgccatag atggttgcgc tcttcagctt ggtgcccgcg gataacgttt
     1081 tgatgacatc gttgatctgc tctgctaatt cacgcgtcgg tgtgacgatg agtgcacgcg
     1141 gcgtgttgcg ttgaccattg agcaatttgt tgagaatagg caatacaaag gctgcggttt
     1201 tgccggtgcc agtttgtgct gtaccaataa tgtcacgtcc gcgaatggcg gctgggatgg
     1261 cagcttcttg aatgggggtg ggggtctcat accccgcctt cataattccc tgcttgaggc
     1321 gggggtcgag attaaaattg ttgaagttca tgatttcctt tttaggtgac agtcactata
     1381 cgtgatcgtc ccctgattct ttgtatgaag cattatatcg cttatgtttc gattgaacgc
     1441 atttctgagc gtgtaagaat cacaactgca atgagaatca gtgctccgcc agccaatacg
     1501 atcggtgcaa gtttgtcgcc aaataagacc gttgcgagaa aaaccgtgat gatgggttca
     1561 atagtcgaga gcattgccgc attggtagga ccgatccgct ccaaaccagc aagaaaagtt
     1621 gtgaccggga taatggtgga cacgacaaca atgcccgcaa ttaacagcca accgaagttc
     1681 gtctgtggaa gatgaattcc gttggtgaaa gtcaacagtc catagaccac actcgcggaa
     1741 gcgaaaatta cggtcgacga ctgaaatggg ctgacatgtt tcatcacatt ggcaccgacg
     1801 atgatgtaaa tggaataccc caatgcggca acaattgcca taattgcgcc aatgggctgt
     1861 ccactgacgg gaccgaccgt tagtgcggtg ccgcataatg ccagggcaag cgcaaaggct
     1921 tttacgcggg tgagtttctc ttgcaagaag atcatcgata agatggcaac aaaaaaggga
     1981 tacagataca aaagcaaggc gaccaaaccg gcagaagcat atttgatagc ggttaggtat
     2041 agaaacgatt gccctgcata cccaatgcca cccatgccga ttagttgggc gaggatgcgt
     2101 ccgcgtggga aaggctcctt gcgcagaatc aacatcatgg tcatgaatga ggcggaaaat
     2161 ccaaaacgga gaaaaaggag ggtgaaggtg tctatgtttt cagcgtaggc atagcgccca
     2221 aagatggcaa gcgtcccaaa tgatgcggca gaaatggcga tcaaaatgat tccggtgagt
     2281 cgtctcatag cgggcaacta taccgcactc aatcgtaaat tgtgcttttt aaatgcagta
     2341 tatacccgaa aaaaactccc cgttgaaggg gagtttatgc tgaaaccgcg gctcgaaaca
     2401 gatttatttt gtgatgtgaa aacccattcc aaatctcttg cggataaacg atttgatttg
     2461 tgccaggcag ttgctccatg gcgagacccg tgttcaagtt aatgccgatg gtttgatagg
     2521 ctttgtatgc gagctggcta caataaaaag cctcttcggt ttcggagtta aagaagttta
     2581 gattgtaggg cttaccaact tgcgcaagga tatattgggc aacagctttg cgtaaatgaa
     2641 attcgtcttc ttccatatac ccaagcacat ccaatggaga gaccacgccg taaaaggtgt
     2701 cgaacaacaa accgcgttgg cgtgccatgc gttcggtgtt ccaaactccg ttgggtacat
     2761 caaaagtgat cacacctttt gccccagcct cggcacagcg cccgtcgggc ccgaggtaga
     2821 tggcgacatg gtccacatcg cccggcacga gccgcccaac caagcgccaa aactcacccg
     2881 gtaagatatc gggcttgccg ttcatggtgc agataatatc gcctgtttgc acagacatac
     2941 cgttgatctg ataactgtaa accgtcatgt tactttatac gaaggtgtac agcaaaagtt
     3001 tctatataaa tgaaagtaga aaatctcgaa tctgattcgc ggtctgattc cacttgggct
     3061 ggcgctgata gcgactccgc gctgcgaggc tcatttttag gaggagatca cggttctcgc
     3121 tcaggagttg cagtttttct gccaacctgt gagcgtctcc cggctggatg agaaacccag
     3181 tcaccgcatc gtcgatgact tctgacgcgg cgccaagagt agtgcctatt gcgggcagcc
     3241 caaagcccat cccttcgagg tagacaatgc cgaaaccttc ataacttgat ggcacaacaa
     3301 ggacatgggc ttgtttgagt ttgtcgacaa gaggttgatt gttgagtgca ccgtgaagag
     3361 aga
//
LOCUS       DAOQNJ010000267         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19492_length_3360_cov_7.008169, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000267 DAOQNJ010000000
VERSION     DAOQNJ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19492_length_3360_cov_7.008169"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLG52_08400"
     CDS             complement(<1..617)
                     /locus_tag="PLG52_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNB41507.1"
                     /translation="MRVRITFTKQGALRYIGHLDLHRVWERALRRASLPISYSQGFHP
                     QPKISLAAALPLGFSSRAEVIDVRLNEEIPTEEIHTRLKDNLPPDIKVTDVKSVDERL
                     PALQTLVLSAAYDVHLTEPVAGSELTRRVESLMQAESIIRERRGKTYDLRPLVEMLSV
                     ITQADGTVWLKMTLAAREGATGRPEEVLTVLEIEPETARVERTRL"
     gene            complement(620..1147)
                     /locus_tag="PLG52_08405"
     CDS             complement(620..1147)
                     /locus_tag="PLG52_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41508.1"
                     /translation="MNKAILLERLQNERDQFELLLNRLGFARQLTMRGVVAGLTVKDL
                     VAEVFSHEQFIADRLSEILHGDTYAPSASFTALENFQREYGYPDYESSLIEKDKHDLS
                     VVDAYKHIEFDEIISQELAVYGNILESIEKLTHHEFLDHDLYNRIAEHTYRPYRRTSS
                     IINRWLKRIASETKK"
     gene            complement(1156..3072)
                     /locus_tag="PLG52_08410"
     CDS             complement(1156..3072)
                     /locus_tag="PLG52_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNB41509.1"
                     /translation="MFTPEQIEAKLERILLKVERPGRYVGGELNSTIKDWDKVQTHVA
                     FIFPDIYDIGVSNVGLKVLYDQVNQREDALAERAYAPWLDMEKLMREHEIPLYALESK
                     RPLACFDLIGFSLPYETLYTNALNILDLAGIPVRNVDRDDSHPIIIAGGHSATNPEPM
                     HAFIDAFVIGEGEEAIHDIINSLQKTKGLPREERLRALLSISGIYVPRFYETTYMEDG
                     TVAATTPTVAEAPKLVNKRIVAKLPPPPTKFIVPNIDVVHNRVSVEIMRGCTRGCRFC
                     QAGMITRPVRERTVQEVVDAADAAVRSTGFEELALLSLSSSDYTYVNDLVDAISKRFE
                     GRKLTVSLPSLRIESVSVDLMEKLKQHRSGGFTLAPEAASERMRRIINKYIPDEDIIN
                     TTRDIYARGWTTIKLYFMIGHPSETIEDVQAIADLCKRVLAEGRKVAGWKVKLHAGVS
                     TFVPKPQTPFQWVACDTRDNILEKQALLKREFFKDKSIKLSWTKPDDTLVEAWLSRGD
                     RRMSEVIYTAWKNGAKFDAWEEGRKFETWMAAFEENGIDHAFYTHRQRRTDEIFPWEH
                     ITAAVRKNFLFQDFRMSLEGQIRVDCRLNCFACGILPTFANLRRENPGEGWKCPEVKT
                     PARKVELELPVAGD"
BASE COUNT          829 a          768 c          866 g          897 t
ORIGIN      
        1 attaggcgag tgcgctctac ccgcgccgtt tccggttcaa tctcaagcac agtcaatact
       61 tcttctgggc gcccggttgc gccttcgcga gcagctagag tcattttcaa ccaaacggtt
      121 ccatctgcct gcgtgatcac acttagcatt tcaaccaacg gacgcagatc ataggttttg
      181 ccacgacgtt ctcggataat agattcagcc tgcatgaggg actcaaccct acgcgtcagt
      241 tcggacccgg caactggttc cgtcaaatgg acatcatacg ccgctgataa caccagtgtt
      301 tgcaaagcgg gcaaacgttc atccactgac ttcacatctg tcactttaat atcgggcgga
      361 agattatcct tcaaccgagt atggatttct tcggttggga tttcttcgtt taaccgcacg
      421 tctatcactt cagcacggga ggaaaaccca agcggcaatg cagcagctag gctgatcttg
      481 ggttgggggt gaaagccttg tgagtatgaa atgggcagac tggcacggcg taatgcccgt
      541 tcccatactc ggtgcaggtc aagatgacca atatagcgca atgcgccttg tttggtgaat
      601 gtaattcgaa ctcgcatatt tatttttttg tctctgaggc gatgcgtttt agccagcggt
      661 tgatgatgga actggtacgg cggtaggggc gataagtgtg ttccgcgatg cgattgtaca
      721 ggtcgtggtc gagaaattca tgatgtgtca gtttttcaat cgactctagg atatttccat
      781 atactgccag ttcttgagaa atgatttcat cgaattcgat atgtttataa gcatctacca
      841 cagataggtc atgcttatct ttttcgatca acgaagactc gtaatcagga taaccgtatt
      901 ctcgctgaaa attttcgagg gctgtgaaag aagctgatgg agcataggta tcaccatgca
      961 gaatttcgct taaacggtct gcaatgaatt gctcatgtga aaaaacttct gctacaagat
     1021 ctttgacagt aagcccagct accactccgc gcatggttag ttgacgcgca aaaccaaggc
     1081 gattcagtaa aagttcaaac tggtcgcgtt cattttgcaa gcgttcgagg aggatggctt
     1141 tgttcatgct tgtttctaat ccccagcgac gggaagttcc aattctactt tacgggcagg
     1201 ggttttcact tcggggcatt tccaaccttc accggggttt tcgcggcgta agttcgcaaa
     1261 agtaggcaga attccgcaag cgaaacaatt caagcgacag tccacgcgga tctgaccttc
     1321 gagcgacata cgaaaatctt ggaagaggaa gttcttacga acagcggctg tgatatgttc
     1381 ccatgggaag atttcatccg tacggcgttg acgatgagta tagaaggcat ggtcaatgcc
     1441 attttcttcg aaggcagcca tccacgtttc gaatttgcgc ccttcttccc aggcatcgaa
     1501 tttggcaccg tttttccatg ctgtgtagat cacctcagac atgcggcggt cgccacgaga
     1561 aagccaggct tcaacaagag tgtcgtcagg ctttgtccag cttagtttga tacttttatc
     1621 cttgaagaat tcacgtttga gcaaggcttg cttttcgaga atattgtcgc gtgtgtcaca
     1681 ggcaacccat tggaaaggtg tttgtggttt aggcacaaaa gtgctgacac cagcatgtaa
     1741 cttcactttc caacccgcca ctttacgtcc ttcagcaagg acgcgtttgc acagatctgc
     1801 aatggcttgt acatcttcaa tcgtttcact ggggtgaccg atcatgaagt agagtttgat
     1861 agtcgtccaa ccgcgggcgt aaatatcgcg agtggtattg atgatatctt cgtcaggaat
     1921 gtacttattg atgatacggc gcatgcgttc actggcagct tcaggagcaa gagtaaaccc
     1981 gcctgagcga tgctgtttga gcttttccat taaatctacc gaaacagact cgatgcgcag
     2041 agaaggcagc gagacagtca actttcgacc ttcaaaccgt ttgctgatgg catccacaag
     2101 gtcgttgaca taagtgtagt ctgatgagga tagcgacagc agcgctagct cttcaaaccc
     2161 ggtagagcgg actgcagcat cagcggcatc taccacttct tgtacggttc gttcgcggac
     2221 cgggcgagtg atcatccctg cctggcagaa gcgacagccg cgggtgcatc cgcgcataat
     2281 ttcgaccgaa acgcggttat ggactacatc aatatttggg acgatgaatt ttgtaggtgg
     2341 tggcggcagt tttgccacaa tgcgtttgtt aaccagtttt ggagcttctg caacagtggg
     2401 cgttgtggct gcaacagtgc cgtcctccat gtatgttgtt tcatagaaac gcggtacata
     2461 tatgccagaa atacttagca atgcacgcaa acgttcttca cgcggtaagc cttttgtctt
     2521 ttgaagcgaa ttgatgatgt cgtggatggc ttcttcacct tcgccgatca caaacgcatc
     2581 aatgaaggca tgcattggct cagggttggt ggcggaatgt ccgccggcga tgatgatggg
     2641 atgcgagtcg tcacgatcta cgttgcggac ggggatgcca gcaaggtcta gaatgttgag
     2701 cgcattagtg tagagtgtct cgtaggggag agaaaagccg atgaggtcga aacaggctaa
     2761 aggtcgtttt gattcgagcg cgtacagcgg aatctcgtgt tcgcgcatga gcttttccat
     2821 gtctagccag ggggcgtagg cgcgctcagc aagcgcatcc tcgcgttgat tgacttggtc
     2881 atacaacact tttaaaccga cattggaaac gccgatatca taaatatcag ggaagataaa
     2941 ggcaacatgg gtttgcactt tatcccaatc tttgatggtg gagtttagtt caccgcctac
     3001 atagcggccg gggcgttcaa cctttaagag gattctctcg agcttggctt caatttgttc
     3061 tggagtaaac atgccgccga ttatacctga taagaaatgt ccggttggtc cggtggggat
     3121 gtgcttgtgg ctaggtgtac tttgcatttc tttgcagaat tgataaggaa tccatctttg
     3181 aaatagcccc aaggggtcaa ctaggaaaaa tggacttggg catagaatgg aataaattta
     3241 caaaggagtt tttatgaaaa aagcactcac tattaccata gccctgctcc tgacggcatg
     3301 cggcgttgta cagccagcca ctcaggaagt attgccaacg ccgattgtgc aaactgttgt
//
LOCUS       DAOQNJ010000268         3350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19592_length_3350_cov_8.333839, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000268 DAOQNJ010000000
VERSION     DAOQNJ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19592_length_3350_cov_8.333839"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLG52_08415"
     CDS             <1..277
                     /locus_tag="PLG52_08415"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB41510.1"
                     /translation="GGSQAISRSLFAQMIPNGKEAEFFAFYEISERGTSWIGPLVFGL
                     ANQMFNNLRVGILSLIFFFIVGLVLLPFVNVNKAIADAKKYDKNAVS"
     gene            complement(321..1421)
                     /locus_tag="PLG52_08420"
     CDS             complement(321..1421)
                     /locus_tag="PLG52_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41511.1"
                     /translation="MDTAFHIIVFLLGAGLVIATIFSAISTFVLPRSARSQLNRLVFG
                     LTRRFIEIFMHFARTYEQRDAIMAYYAPLALMSLVPTWYMLIALGYSFMYWSLRAGDY
                     FFDLRLSGSSLFTLGFTASEGYFITILAFSEAMLGLIMVGLLIAYLPTMYSAFSRREQ
                     AVNMLEVRAGNPPNPYDMLSRFHRIHGLDKLAELWKNWEIWFADVEESHTTLPALVFF
                     RSPRSDNSWVTSAGAVLDSAAIALSSIDIPSEMSGALCIRAGFICFHRITDYFDIPHP
                     HDPKYPETPISVTRAEYDELIHKLEVAGVPIKPDREQAWKDFAGWRVNYDRTLLIMCA
                     LVMPPSNVPWSSDRAPKFKIPPLFIKKTKHQH"
     gene            complement(1488..1712)
                     /locus_tag="PLG52_08425"
     CDS             complement(1488..1712)
                     /locus_tag="PLG52_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41512.1"
                     /translation="MAPMVHGLEAEYFGRVKFSFLDADDPNTYNFQRELGFVYQPEFY
                     LLDANGNVLKKLVGPVSEEEFRDLFDTNLQ"
     gene            complement(1728..2015)
                     /locus_tag="PLG52_08430"
     CDS             complement(1728..2015)
                     /locus_tag="PLG52_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41513.1"
                     /translation="MKPRLMFWTLGLVIALLAIACAPSNPTETAPPVVEATATVASAP
                     LVETAVPQVEATIPAVLPVATSRGPNLEATDPATVSFASGGLQLIEFFRFT"
     gene            2248..>3350
                     /locus_tag="PLG52_08435"
     CDS             2248..>3350
                     /locus_tag="PLG52_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB41514.1"
                     /translation="MANFKLTYATMFNPPEELHTGFDKAVAKLKENMGKEYGMFINGK
                     EVFADEKFEDRSPINTDWVLAKMQKGNASHAQMAVDAARKAFPMWSRTPWQQRVKLLR
                     KAASLIEDRIFELGAAMAMEVGKNRMESMGDVQETADLIYYSCYQMEKNNGYIAEMGR
                     DPLVGYESRNVSVLRPYGVWLVVSPFNFPHALTGGPSGAALAAGNTLVIKPATDTAWI
                     VRLLVDCFRDAGFPEGVVNFVTGPGSTLGQALVDSPEVDGVTFTGSFDVGMKMYRDYA
                     NRNYVRPMILELGGKNPAIVSRNADLETAAVGIVRSAFGLQGQKCSAASRVLVEEPVY
                     DELVARLKDLTDKLVLGDPTNRATYNGPVINKSS"
BASE COUNT          912 a          814 c          856 g          768 t
ORIGIN      
        1 gggtggttcg caagctatca gccgcagttt gtttgcgcag atgatcccca atggcaagga
       61 agcagaattc tttgctttct acgagatcag cgaacgcggc acctcatgga tcggaccgtt
      121 ggtctttggt cttgccaacc agatgttcaa taacttgcgt gtgggtattt tatctttgat
      181 cttcttcttc atcgtaggct tggtcttgct gccgtttgtc aacgtcaaca aagccattgc
      241 agatgcaaag aagtacgata aaaacgccgt aagctaaata ataaaaagag tgccgaggta
      301 tgtctcggca ctctttttat ttaatgttga tgctttgttt tctttataaa caatggaggg
      361 attttgaatt tcggagcccg gtcgctggac catgggacgt tagagggtgg catgaccaac
      421 gcgcacataa tcagcaatgt acggtcatag ttgactcgcc atccggcgaa atctttccaa
      481 gcttgttcgc ggtctggctt gatgggcaca ccggcaactt ccagtttatg aatcaactcg
      541 tcatattcgg cgcgagtgac actgattggc gtctctgggt atttggggtc gtgcgggtgt
      601 ggaatatcaa aataatctgt gatgcgatga aaacaaataa aaccagcccg aatacacaaa
      661 gcaccagaca tttcggaagg aatatcaatt gatgaaaggg caatggcagc cgaatctaaa
      721 acggctccag cagaagtgac ccaagaatta tcggaacgag gcgagcgaaa aaagaccaaa
      781 gcgggcagag tggtatgact ctcttccacg tcggcaaacc agatttccca gttcttccat
      841 agttctgcga gtttatccaa accgtgaatg cgatgaaagc gggagagcat atcgtaagga
      901 ttcggcgggt tgcccgcgcg gacctccaac atattcaccg cctgctcacg gcgggaaaaa
      961 gcagaataca tcgttggcaa ataagcgatc agcaagccca ccatgatgag tcccagcatg
     1021 gcttcggaga aggcgaggat ggtgatgaaa tatccctctg aggcggtaaa tcccaatgta
     1081 aataaagaag agccgctcaa gcgcagatca aagaaataat ccccagcccg taaagaccaa
     1141 tacatgaaag aatacccaag cgcaatcaac atgtaccaag taggcacaag cgacatcagt
     1201 gccaaaggtg cataatacgc catgatggca tctcgttgct cataggttcg agcaaaatgc
     1261 atgaagattt caataaagcg acgggttagc ccaaacacca gccggttaag ttggctgcgt
     1321 gccgaacgtg gcaaaacgaa tgttgaaata gcagaaaaaa tggtagcaat gaccaaccct
     1381 gcgccgagca agaaaacgat gatgtgaaag gctgtatcca tgagccggaa ttatattgga
     1441 gttaaacaaa aaagagacag tcagttgact gtctctttta aacctgctta ctgcaaattc
     1501 gtatcgaaca aatctctaaa ttcttcttca gaaacgggac cgaccaattt cttcaacaca
     1561 tttccatttg catctagtaa ataaaattcg ggctgataca cgaacccgag ttcgcgctgg
     1621 aaattataag tgttgggatc gtcggcatcc agaaaactaa acttgaccct gccaaaatat
     1681 tctgcttcaa gcccatgaac catgggcgcc atggcgcggc aggtacttca ggtaaagcgg
     1741 aagaattcga tcagctgcaa accgccagag gcaaagctga cggtggcggg gtctgtcgct
     1801 tcgaggttgg gaccgcgaga cgtggcgacc ggcaaaacag caggaatagt cgcctccacc
     1861 tgggggacgg ctgtttccac gagcggagcg gatgccacgg tggcggtcgc ttctacaacg
     1921 ggcggagcgg tttcagtagg gttggacggg gcgcaggcaa ttgccagaag cgcaattacc
     1981 aagcctaagg tccagaacat gaggcggggt ttcatcagtt ttctccttat taaagttaat
     2041 taaatgatac caaggcactg catcctctta caaaattcac acaaacttct catgaggtat
     2101 gtcacaggca aaacagtcgc tttactttga cgacggttaa atactacgct atacttaatt
     2161 tgcactttgt ctcacaatag ggtcacttgc tcagggtgac acgagataat ttgcagattt
     2221 gtctcacact tctaaaggag aactcctatg gcaaatttca agctcacgta cgcaaccatg
     2281 ttcaacccac ctgaagaatt gcacaccggc ttcgacaaag ccgttgccaa acttaaggaa
     2341 aatatgggca aggaatatgg catgttcatc aacggtaagg aagtttttgc cgatgaaaag
     2401 ttcgaagacc gctcgcctat caacaccgat tgggtgctcg ccaaaatgca aaaaggcaac
     2461 gccagccacg ctcaaatggc agtggatgcg gcgcgcaaag ccttcccgat gtggagccgc
     2521 actccctggc agcaacgtgt aaaactgctc cgcaaggctg cttctttgat cgaagaccgc
     2581 atctttgaac tcggcgccgc catggcaatg gaagttggca agaaccgcat ggaatctatg
     2641 ggcgatgtgc aggaaactgc cgaccttatt tattattcct gctaccaaat ggaaaagaac
     2701 aacggctaca ttgcagaaat gggacgcgac ccgttggtgg ggtacgaatc tcgtaacgtg
     2761 tctgtattac gcccttatgg tgtgtggctt gtggtgtcac cgttcaactt cccgcacgcg
     2821 ctcacaggcg gaccttcggg cgccgctctg gctgcgggca acacccttgt catcaaacct
     2881 gcaactgata cggcttggat tgtgcgcttg ctagtagatt gcttccgtga tgcaggcttc
     2941 cctgaaggtg tggtcaactt tgtgacgggc cccggctcga ctctgggtca ggcactggtc
     3001 gacagccccg aagtcgacgg cgtcaccttt accggttctt ttgatgtcgg tatgaagatg
     3061 tatcgtgatt acgccaaccg caattatgtg cgcccaatga tcctcgagct gggcggtaag
     3121 aatcctgcca tcgtttcccg caacgcagac cttgaaactg cggctgtggg gattgtccgc
     3181 tcggcatttg gcttgcaggg acagaaatgc tcggcggcgt ctcgcgtgtt ggtggaagaa
     3241 cccgtctatg atgaactggt tgcccgcctc aaagacctga ccgataaact cgtgctcggt
     3301 gacccaacca accgcgccac ctataacgga cctgtgatca acaagagttc
//
LOCUS       DAOQNJ010000269         3342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19660_length_3342_cov_7.700943, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000269 DAOQNJ010000000
VERSION     DAOQNJ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19660_length_3342_cov_7.700943"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="PLG52_08440"
     CDS             <1..1880
                     /locus_tag="PLG52_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB41515.1"
                     /translation="KGEIRCEIIVNAAGMWAPRIAAMAGLHVPTTPVDHQHIALRAVP
                     GHEFNDQTPCLRDPDNLVYMRQEKGGLVIGGYEPKPLPRWIDGTPWEHGSKSFPGDFD
                     QFEMLLEGAIRRLPFLDQAGIITLVRHPGAYTPDCQPLLGPMPGVKGFWMMAGMSLNG
                     YGGAGGMGKLMAEWIMDGEAPMDVYGYRATRFGNYYSDFNYAAERTVEAVKYYYRLKF
                     PHDESEAARPHRTSPVHYRLMENGAVFGEKFGWERVNYFNPGNEWRRMGEDQREWGWT
                     KPPYFERMRVEHIATRERVALFDLTSFGKIEVKGPGALPLLQRLTSSNIDKPVGSAVY
                     TQFLNRNGGIESDLTVTRLGEAYFWVITGSGFIANDHARILMNVNEADGEVSIRDITQ
                     EHACLALWGPKARDVLKKITSSDVSNEGHPYLTTKPIQINGVKVIAQRVSYAGELGWE
                     LYIPNNRATMVWDMLVDAGKEFGMELGGYKVLDPLRLEKGFKYFTTDITPTTTPYEAG
                     LGFCVDLNKGDFIGRDALVKQKADGVTRKLCTLVLTDTDEFVQIYGGEAVHHEGKVVT
                     RIRSGGYGFTVKKNILYAYLPVDLAVKGTRFTIELIEGNLAAEVTANVLYDPKSEKLK
                     A"
     gene            1899..2990
                     /locus_tag="PLG52_08445"
     CDS             1899..2990
                     /locus_tag="PLG52_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNB41516.1"
                     /translation="MAKKNSSPAYIGFGMLTPVYIMSLDQLPKLNTGAIVHQVSDYVY
                     DDAAIIACNLSQWNVPSAMIGTAVGKDLLGKYVADKLKELGVKGEVRFTTKYKTPLEV
                     NVSDKKGARTYFWQRSKEILDTLSTADLSLIKSAKLLYVDWYDGEHIVRAMAEAKKHN
                     VPVFLNFEHGHQNPDLLKKYAGMVTVCQAVTDAAQIGKRQAMLGTARKLIKSGIQTAI
                     ITMASQGCMVVQGDEIIRAYAPKVKAVDASGAGATFSSGYIYGYLNGWSLEDTVRFAI
                     AAASLKVTRSGLDMFPVSEIKGLARMVRIERMVYRGDQFRTIRKLLTLPENSPLVNNA
                     LVKESQKLAERILPKKKIERKKIKRSLVE"
     gene            3014..>3342
                     /locus_tag="PLG52_08450"
     CDS             3014..>3342
                     /locus_tag="PLG52_08450"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNB41517.1"
                     /translation="MSFAALLTQEQVEKIHDASLEILEDVGLKVRYEPARELFKKHGC
                     MVEDERVKFPRAVVEKYRKLVPSSFTFHARDPKFDKTIPQDSPVIVTASSAPDIIDPV
                     TGKERRA"
BASE COUNT          853 a          712 c          916 g          861 t
ORIGIN      
        1 acaaaggcga gatccgatgc gaaatcattg tcaatgccgc aggaatgtgg gcgccgcgca
       61 tcgctgctat ggcgggtctg catgttccga ccactccagt tgaccatcaa catattgcgt
      121 tgcgagctgt gcctgggcat gagttcaacg atcagacgcc ttgcttgcgt gacccggata
      181 atttggtgta tatgcgtcag gaaaaagggg ggcttgtgat cggtgggtat gagccgaagc
      241 cgcttccacg ttggatcgat ggtacgccgt gggaacatgg cagtaaatct ttccccggtg
      301 atttcgacca atttgaaatg ttgcttgaag gtgcgattcg tcgccttccg tttttggatc
      361 aggcgggcat cattacgtta gtacgtcacc ccggtgcgta cacgcccgat tgccagccct
      421 tgctcggacc gatgcccggt gtgaaaggtt tctggatgat ggcgggcatg tcgctcaatg
      481 gatatggtgg cgcaggtggc atgggcaaac tgatggcgga atggatcatg gatggtgaag
      541 caccgatgga tgtgtatggc tatcgtgcca cacgctttgg taattactat tctgatttca
      601 actatgccgc cgaacgaacg gtggaagccg taaagtatta ctatcgtttg aaattcccgc
      661 acgatgaatc ggaagcggca cgtccgcatc gcaccagccc ggtgcattat cgtttgatgg
      721 aaaatggcgc ggtttttggc gagaagtttg gctgggagcg cgtgaattat ttcaacccag
      781 gtaatgagtg gcgtcgtatg ggcgaagatc aacgcgagtg ggggtggacg aaacctccat
      841 acttcgaacg gatgcgagtg gaacatattg caacccgtga gcgtgtggca ctgtttgacc
      901 taacctcttt tggcaagatc gaagtcaaag gacccggtgc attgcctttg ttgcaacgct
      961 taacgtcgag taatattgat aagcccgttg gcagcgcggt gtatacccaa ttcttgaatc
     1021 gcaatggcgg tattgagtct gatttgacgg tcactcgttt gggtgaagca tatttctggg
     1081 ttattacagg ttctggtttt attgccaacg accatgcgcg gattttgatg aacgtgaatg
     1141 aagcagacgg cgaagtgtct atccgtgata ttacgcagga acatgcctgt cttgccttgt
     1201 ggggacccaa ggcgcgtgat gtattaaaga agattactag cagtgacgtg tctaatgaag
     1261 ggcatcctta tctgaccaca aagccgattc aaattaacgg cgtgaaagtg attgcacaac
     1321 gagtaagcta tgctggcgaa ttgggctggg agttgtacat tccgaacaat cgcgccacca
     1381 tggtatggga tatgcttgtt gatgctggta aagaatttgg catggaactc ggcggctata
     1441 aagtgctgga cccgctacgt ttggaaaaag gctttaagta tttcacgacc gatattaccc
     1501 caacaaccac accctatgaa gctggtcttg gcttttgcgt ggacttgaac aagggtgact
     1561 tcatcgggcg cgatgcgttg gtcaagcaaa aagcagatgg agtaacgcgg aaactctgta
     1621 ctttggtatt gaccgatacc gatgaatttg tgcagatcta cggcggtgaa gcggttcatc
     1681 acgaagggaa agttgtcact cgtatccgca gtggcggcta cggctttacc gtgaaaaaga
     1741 atattttgta tgcctacctg ccagtggatt tggcagttaa aggcactcgg ttcaccattg
     1801 aattgatcga gggcaatctc gctgctgaag tgactgccaa tgtcttgtac gacccgaaga
     1861 gtgagaagct gaaagcgtaa attgattacc ctctaattat ggctaagaaa aattcctctc
     1921 cagcttacat tggctttggc atgttgacgc ctgtgtacat tatgtcgctg gatcaattgc
     1981 ccaagttaaa tacaggtgcc atcgtgcatc aggtcagcga ttatgtgtat gacgatgctg
     2041 ccatcattgc ttgcaacctt agccagtgga atgtaccgtc tgccatgatc ggaacagcgg
     2101 tggggaaaga cttgcttgga aaatacgtcg cagataaatt gaaagaattg ggcgtaaaag
     2161 gggaagtgcg ctttacgacg aaatataaaa cgcctttgga agtgaacgtc tctgacaaaa
     2221 aaggcgcgcg cacgtatttt tggcaacgtt caaaggaaat tctcgataca ttgtccacag
     2281 cggatttgtc actgatcaaa tctgcaaaac tattgtatgt ggactggtac gatggcgagc
     2341 atatcgtccg tgccatggca gaagccaaga aacataatgt gcccgtcttt ttgaactttg
     2401 agcatgggca tcagaaccca gacctgctca aaaaatatgc gggcatggtc acggtctgtc
     2461 aggcagtgac cgatgcggcg cagattggca aacgtcaagc tatgttaggc acagcgcgca
     2521 agctcattaa atctggtatt caaacagcta tcatcactat ggcaagtcag gggtgtatgg
     2581 tggtgcaagg tgatgaaatt atccgcgcct atgcgcccaa ggttaaggct gttgacgcct
     2641 caggagcagg cgccacattt tcatctggct atatttatgg ttatctcaat ggctggagct
     2701 tggaagacac ggttcgtttt gcaattgctg cagcgtcttt aaaagtgacc cgttctgggt
     2761 tggatatgtt cccagtatct gaaatcaaag gattggctcg catggttcgt attgaacgca
     2821 tggtgtatcg cggagaccaa ttccgtacta ttcgtaagtt gttgaccctt ccagagaaca
     2881 gtccattggt taacaatgcg ctcgtgaaag aaagtcagaa gctggcggaa agaattttgc
     2941 ctaagaagaa aatagagcgt aaaaagatca aacgctcact ggttgaataa tttattttat
     3001 aaggatgtga accatgtcat ttgctgcttt gcttacgcaa gaacaagtcg aaaagattca
     3061 cgatgcttcg ttagagattt tggaagatgt tggcttaaag gtccgctatg aacctgctcg
     3121 cgagttgttc aagaaacatg gctgtatggt cgaagatgag cgtgtgaaat tcccacgcgc
     3181 ggttgtggag aaataccgca agttggtgcc gtcatctttt acctttcacg cacgtgaccc
     3241 gaaatttgat aagaccattc cgcaggatag cccggtcatt gttacagcga gttccgcgcc
     3301 cgatattatt gatccagtga ccggcaaaga aagacgcgcc ga
//
LOCUS       DAOQNJ010000270         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19701_length_3337_cov_6.001828, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000270 DAOQNJ010000000
VERSION     DAOQNJ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19701_length_3337_cov_6.001828"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PLG52_08455"
     CDS             <1..977
                     /locus_tag="PLG52_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41518.1"
                     /translation="MVDDDEVATAIIIPAGFTDSIIPPTEMGMTIYVGPKLIEMYANP
                     SRPTSAGIIKSIVDEFMSRVDESTVSGQTAIQQLLTSGRVPPEQAQVAGTELNQRLTN
                     SQQDEPLAIKLNATTADGEEIKFDVLAYLAPGMALMFLMFTVSYGGRSILAEKSQGTL
                     PRLLVSPTNSTQILGGKVFGIFLTGVAQMLILIGGTSLMFQLKWGDTLGVLVLVFAAV
                     FGATGWGMLITALAHTPAQVGSVGSATMLIFGILGGSFIQLDNMPAAVQTLSRITPNA
                     WALDGFTTLALGGTLTNLSTPITALLTMGGVLFVISVLLFGKKNLVQK"
     gene            974..2173
                     /locus_tag="PLG52_08460"
     CDS             974..2173
                     /locus_tag="PLG52_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41519.1"
                     /translation="MKKIFAIAWKDAVVRFASSSELLFFIILPLVFTFLLAGGSPSGN
                     PTNNRVRLVVVDQANTAISQEIVAELEKSTAAYPELMSLADAEEEFSARRVSAVLVIP
                     AGLEISAIQNGTAELDFRQQPNDMDASIAQRAIQTAIRRVSSAVNAANVAVEEAKARG
                     TFESAEAEQAYFEEALKVAKDLQANSPERITVIEGSTPDKVDYDPRANTSAGQLVTWV
                     FIPLFGISALFAYERQQGTLRRLLTTPTSKAVFLLGTIFGQVVMALIQMSLLILFGMF
                     ALQLKWGQEPLALFILLTCAALAAGAIGTTMGTFIKSEGQASGLSIMAGMVMALLGGC
                     WYPLELFPAAVQNIVKILPTTWAMQGLLDLVLRGGGLTDILLESGVLLGFAVVFFSIG
                     VARFRYE"
     gene            complement(2224..2751)
                     /locus_tag="PLG52_08465"
     CDS             complement(2224..2751)
                     /locus_tag="PLG52_08465"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNB41520.1"
                     /translation="MAKNIVLTLTGKDRIGLVDQVTSLVVKRSGNVESSRMARLGGEF
                     AMLMLVTVSDAEFQNLDKDFQEMRGEGYQVTLMQTEDVSKKYAGWLPYEIEVTGADHE
                     GIIQQISHHLASQGINIESMDTSATPAPMSGTPLFMMKGIVLVPPKLNFNAWSDALEE
                     VGDTLNVSVKIEVVK"
     gene            2836..>3337
                     /locus_tag="PLG52_08470"
     CDS             2836..>3337
                     /locus_tag="PLG52_08470"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNB41521.1"
                     /translation="MQTFLKFSRTIDALTDKLGHASAVCVMLTIAVGFYNVIARYLGR
                     FFEIKLTSNLFIELQWYLFSLTFLLGFSYVLKHGLNVRVDFLYTNWSPKTKAWVDLVG
                     HAFFLTAFCIIGLYVTTHPVLQSWGLLPDGSFGSWELSPDPDGLPRAPIKSMMLVAFG
                     MLFLQTI"
BASE COUNT          753 a          898 c          793 g          893 t
ORIGIN      
        1 agatggtgga cgatgacgaa gttgctactg ccatcatcat ccctgccggg tttacagata
       61 gcatcatccc gcccactgag atgggcatga ccatttacgt cggaccaaag ctgatcgaga
      121 tgtatgccaa tccatcacgc ccaaccagcg caggcatcat caaatcgatc gtggacgagt
      181 tcatgagccg cgtggatgaa agcaccgtta gcgggcagac tgcgattcag caattactca
      241 ccagcgggcg cgttccgcca gagcaagcac aagtggctgg cacagagttg aatcaacgcc
      301 tcacgaacag ccagcaagac gagccgctcg cgatcaaact caatgcgacc actgcagatg
      361 gtgaagaaat taagttcgat gtgcttgcct atcttgctcc tggcatggcg ttgatgtttc
      421 tgatgttcac cgtcagctac ggcgggcgtt ccatccttgc agaaaaatca caaggcacgt
      481 tacctcggct tttggtctcc cccaccaact caacgcaaat tcttggaggc aaagtctttg
      541 gcatcttcct cactggcgta gcacaaatgt tgattctaat cggcggcaca tcgctcatgt
      601 ttcaattgaa atggggtgat acccttggtg tacttgtgtt ggtatttgcg gctgtcttcg
      661 gtgctaccgg ctggggcatg cttatcactg cgctggctca tacgcctgca caagtaggca
      721 gcgttggttc tgccacaatg ttgatcttcg gcatccttgg cggaagtttc atccaattgg
      781 ataacatgcc agctgccgta caaaccctta gccgtattac tccgaacgcc tgggcattgg
      841 atggattcac aaccctcgct ctcggtggga cgctcacgaa tctttccacg ccgatcactg
      901 ctctgctaac aatgggcgga gtcttatttg taatttccgt tttgctgttt ggcaaaaaga
      961 acttggtgca aaaatgaaaa aaatattcgc cattgcatgg aaggatgcag tcgtccgttt
     1021 tgccagttca tctgaactgc tgttctttat tatcttgcca ctggtcttta ccttccttct
     1081 ggcgggcgga agcccatccg gcaacccgac caacaatcgc gtgcggttgg ttgtggtgga
     1141 tcaagccaac accgccatct cgcaagagat cgtggcagag ttggaaaagt ccactgcggc
     1201 ataccccgag ttaatgtctc tggcagatgc cgaagaagaa ttcagtgcgc gccgtgtttc
     1261 ggcggtgttg gtgatccctg ctggacttga aatttctgcc attcaaaacg gaactgccga
     1321 acttgacttt cgccagcaac ccaacgacat ggatgcttcc attgcgcaac gtgccattca
     1381 aacggcgatt cgacgtgtga gcagtgctgt caacgccgca aacgtagctg tggaagaggc
     1441 gaaagcgcgc ggcacgtttg agtccgcgga ggcagagcaa gcctattttg aagaggcatt
     1501 gaaagttgcc aaagaccttc aagccaattc ccctgaacgt atcaccgtca ttgaaggaag
     1561 cacaccagat aaagtggact atgatccgcg tgccaataca tcggcagggc aattagtgac
     1621 ttgggtattc attcctttgt tcggtatctc ggcgctcttt gcttacgaac gccaacaagg
     1681 gacattacgt cgtttgctca ctaccccaac cagtaaagcc gtttttctgc ttggcaccat
     1741 ctttggtcaa gtagtgatgg cactcatcca aatgtcgctg ttgattctgt ttgggatgtt
     1801 cgctctccag ctcaaatggg ggcaagagcc gcttgcgctc tttatcttgc tcacctgtgc
     1861 cgcgctcgcc gccggagcga ttggcaccac catgggaacg ttcatcaaaa gcgaaggtca
     1921 agccagcggg ttgagtatta tggcaggcat ggtgatggca ttgctgggtg gttgttggta
     1981 cccgttggag ttattccctg cggcggtaca aaacatcgtc aagattctcc ctactacttg
     2041 ggcaatgcaa ggtttgcttg acctcgttct gcgcggcggc ggactcaccg atatcctact
     2101 tgaatcaggc gttttgctcg ggtttgcggt tgtgttcttc tctattgggg tcgcaagatt
     2161 tcgatacgag taacacttaa aataaaaacg gcggatgatg ttcatccgcc gtttgctttt
     2221 cttttatttc acaacttcaa tcttcacact gacattcaag gtgtccccta cttcttccaa
     2281 tgcatcgctc caggcattga agttcaactt cggcgggaca agcacaatcc ctttcatcat
     2341 aaagagcgga gttccgctca tcggtgcagg cgttgcactg gtatccatgc tttcaatgtt
     2401 gataccctgt gaagccagat gatgtgaaat ttgttgaatg atgccttcat ggtcggcacc
     2461 ggtcacttcg atctcatagg gcagccaacc tgcatacttc ttagaaacat cttcggtctg
     2521 catcaaggtg acttgatagc cttcgccgcg catttcttga aaatctttat cgagattctg
     2581 aaattctgcg tctgatactg tgaccaacat caacatggca aactcgccac ccaaacgcgc
     2641 catgcggctg gattctacat tcccactccg cttcacaacc agactcgtca cctgatccac
     2701 caacccgatc cgatctttgc ctgtgagagt taatacaata ttttttgcca tggcagaact
     2761 ccctttcgac ttgtgaaata tagtacccag tatagcacaa gcacctcgcg tttttatgct
     2821 atcatccgcc aatccatgca gacctttctc aaattttccc gcaccattga tgcgcttaca
     2881 gacaagcttg gtcacgcctc tgctgtttgt gtgatgctca ccatcgcggt cggcttctac
     2941 aacgtcatag cacgttacct tggcagattt ttcgagatca aactcaccag caacctcttt
     3001 atcgaattac agtggtatct cttctccctc acctttctgc tcgggttttc ctacgtcctc
     3061 aagcacgggc tcaacgtgcg cgtggacttt ctttacacaa attggtcacc aaagaccaaa
     3121 gcctgggttg accttgttgg tcacgccttt ttccttacag ccttctgcat tattggctta
     3181 tatgtcacca cacacccggt cttgcaatcg tggggtttgt tgccagatgg ttccttcggc
     3241 agttgggaac tctcgcccga ccccgacggt ctgccgcgtg ccccgatcaa atccatgatg
     3301 ctggttgcat ttggaatgtt gttcttgcaa accattt
//
LOCUS       DAOQNJ010000271         3327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19807_length_3327_cov_9.720660, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000271 DAOQNJ010000000
VERSION     DAOQNJ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19807_length_3327_cov_9.720660"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLG52_08475"
     CDS             complement(<1..770)
                     /locus_tag="PLG52_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HNB41522.1"
                     /translation="MKVFISSTYKDLIDYRAAAIRAVEGTNYQASKMEVFGARPDEPL
                     DACLKEVEESDFFLGIYAHRYGYVPSNLDISITEMEYVHAKKLGKPIYVFVIDEEKQP
                     WLPKFIEGEPGASKLRDFKQRVQTDHVCAFFTTADDLGMKVATALSHYVANHLPTSDS
                     QSPIYQSPKPTGNTLPHQEFFFGREKELESIKASLSPETRAWGALIDGPGGIGKTALA
                     IKAAHDAPAELFERKIFITAKVRELTAEGEAKLTDFTRP"
     gene            1034..1978
                     /locus_tag="PLG52_08480"
     CDS             1034..1978
                     /locus_tag="PLG52_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41523.1"
                     /translation="MTTTIFFATDIHGSDICWNKFLNAGKFYGADQLILGGDMTGKAV
                     VPFIHQGGPNYRVTLLEQVFEITNEDELTEMKKKVRSRGYYPYLTNPDEIKELEKDPE
                     KVSAIFSQEVLKVVQQWMEIAEKKLAGTGMKVYCCPGNDDMDEVDDVIRESRTVVLAE
                     GEVVDLPSGHEMIASGWSNRTPWNTHREEDEDQLAARYEAMISRLKNPQASIFNIHVP
                     PFKSGLDEAPELDKDLRPVLAGNSMIPVGSSALRKAIEKTQPLLSLHGHIHEGRGSTR
                     IGKTLVINPGSMYEQGTLLGAIIKLGKNKVENYVLTTG"
     gene            2024..>3327
                     /locus_tag="PLG52_08485"
     CDS             2024..>3327
                     /locus_tag="PLG52_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNB41524.1"
                     /translation="MSDLFVRKATGMVRSWSVFDAFIYAFFSINLVTLGFYSFSQMYY
                     FGGGMIPALIISAIFIIFEVVVYASLIAVMPRSGGDYVWQTRVFGGGIGFILSITGWW
                     FTLWLWTPIYGDMLRQIVLTPLLGAFGFQQAAVWIAGSGTPLFVCSLLTLLFVALVIF
                     LGMKSYARVQKYSFYAGMLGLLIVLVLLFTGSPEKFKAGLDANSASYFGTSGSLYEPT
                     AQAGTDAFASTPLMGGSFGLIMVTLPYLVFFNLWPNWGATLYGEVRGATDYKRNFSGM
                     AWALVATTLLAIIFFLGVQKTIGWDFYVQSNAAWWNYAWGYVTEAPPLPVWPNPAMLA
                     VFLTDNRLIQIIVLLLMSTWWFGWAGTLFLSSTRVIFAAAFDRLLPEKAAELNDRGTP
                     VNAMLYMILPAILVAALYAYDVAGFKSITLAATQGIAIMYFGT"
BASE COUNT          749 a          803 c          851 g          924 t
ORIGIN      
        1 gggcgggtga agtcggtcag tttggcttca ccttcggcgg tcagttcgcg cactttggca
       61 gtgataaaga ttttgcgttc gaagagttcc gcgggtgcat cgtgtgcggc tttgatcgcg
      121 agcgcggtct tgccgatgcc gccgggtccg tcaatgagtg ctccccatgc tcttgtttcg
      181 ggcgaaagag aagctttgat actttcaagc tctttttctc gaccaaaaaa gaattcttgg
      241 tgcggaagtg tgttgccagt aggtttcgga gattggtaaa ttggggattg ggaatcggac
      301 gtaggtaagt gatttgctac ataatggcta agggcggtag caactttcat tcctaaatca
      361 tctgctgttg taaagaaagc acaaacatgg tcggtttgca ctctttgttt aaagtctctt
      421 aattttgatg cgcctggttc gccttcaata aattttggga gccacggttg tttttcctcg
      481 tcgatgacaa atacataaat tggcttgcca agtttttttg cgtgaacata ttccatctcg
      541 gtgatagaga tgtccaaatt tgaaggaaca tatccatacc gatgagcata aatgccaaga
      601 aagaaatcgc tttcttctac ttctttcaag caagcatcca gcggttcatc gggacgcgca
      661 ccaaagactt ccattttgga ggcctggtaa tttgtccctt cgacagcgcg aatggcagcg
      721 gcgcgatagt cgatcaaatc tttataggtg gaactgatga aaactttcat gtttcatctc
      781 ctgtggtagc ccagtaaaaa cgtgtagaac gaaatgataa ttgtctgcag agaacaggtc
      841 aagccgttat gtaaaactat agcatggaat tttccgaatc ggcgcaggtg ttgtgagaaa
      901 aaatacaaac cgtaactttt tcacctgatt ggggctgtta atattatctt cacatcccct
      961 taatgatgag tataatagcc gtcattaaac cgcaccacct ctgcaagctc atttcatttt
     1021 gaaggatttg catgtgacta caaccatatt ttttgcgaca gatattcacg gttcggatat
     1081 ttgctggaac aagtttctca atgcggggaa gttttacggc gccgatcaat tgatcctcgg
     1141 cggtgatatg acgggcaaag cggttgtgcc ttttatccat cagggcggtc ctaattatcg
     1201 cgtcacctta cttgaacaag tctttgaaat caccaatgaa gatgaactga cggaaatgaa
     1261 aaagaaagtc cgcagtcgtg gctattatcc ctacctcacc aatcccgatg aaattaaaga
     1321 attagagaaa gaccctgaaa aggtcagcgc tattttttcg caagaagtgt tgaaggtcgt
     1381 tcaacagtgg atggaaattg ccgagaaaaa actagccggc accggcatga aggtctattg
     1441 ctgcccgggc aatgacgata tggatgaagt ggatgatgtc atccgcgaga gccgcacggt
     1501 tgtccttgct gaaggggaag tggtcgatct gccgagtggt catgagatga tcgcatcggg
     1561 ttggagcaac cgaacgcctt ggaacacgca ccgtgaagag gacgaggatc aactcgcggc
     1621 gcggtatgaa gcgatgatct ctcgtttgaa gaatccgcaa gcatccattt ttaatatcca
     1681 cgtaccgcca ttcaaatctg gcttggatga agctcccgag ctcgataaag atctgcgccc
     1741 agtcttggcg ggcaactcga tgattccggt tgggtcctcg gcattgcgca aagccattga
     1801 aaagacccaa cccttgctta gtttgcacgg gcacattcac gaagggcgcg ggtccacacg
     1861 cattgggaaa acccttgtga tcaaccccgg ctccatgtat gagcagggaa ctttgttggg
     1921 tgcaattatc aaattgggga agaataaggt tgagaattac gttttgacaa ctggttaaac
     1981 cacgcattcg cgtgtaagac aacatcaaaa gaggagagtt gttatgagtg acctgtttgt
     2041 tcgtaaggcg accggcatgg tacgttcatg gtcggtcttc gacgcgttca tctacgcgtt
     2101 cttctccatc aatctggtga cgcttggttt ttatagcttc agccagatgt attactttgg
     2161 gggcgggatg atcccggcgt tgatcatcag cgcgatattc atcatttttg aagtcgttgt
     2221 ttatgcatcc ttgatcgcgg tcatgcctcg ctctggtggc gattacgtct ggcagacacg
     2281 cgtcttcggc ggcggtatcg gcttcatcct ttccatcaca ggctggtggt tcaccttgtg
     2341 gctctggacc ccgatctatg gcgatatgtt gcgtcagatc gtgctcacgc cattgttggg
     2401 cgctttcggc ttccaacaag ccgccgtgtg gatcgcaggc tcgggcactc cgcttttcgt
     2461 gtgctccttg cttaccttgc ttttcgtagc attggtcatc ttcctcggca tgaagtctta
     2521 cgcgcgcgtg cagaagtatt ccttctacgc cggcatgctc ggtctgttga tcgtgcttgt
     2581 tttgctcttc accggttcac ctgaaaaatt caaagccggt ttggatgcaa actccgcttc
     2641 ctacttcggg acgtctggca gcctctatga gccgactgct caagctggta ctgatgcctt
     2701 cgcctcaacc ccgttgatgg gcggctcctt cggtttgatc atggtcaccc tgccatattt
     2761 ggtcttcttc aacttgtggc ccaactgggg cgcaaccctt tatggtgaag tccgcggtgc
     2821 cactgattac aaacgtaact tctctggtat ggcttgggct ttggttgcca ccacattgtt
     2881 ggcgatcatc ttcttcctcg gcgttcaaaa aaccattggc tgggatttct acgttcaatc
     2941 caatgccgcc tggtggaatt acgcctgggg ctatgtcact gaagcgcctc cgcttcctgt
     3001 gtggcccaac ccggcaatgt tggcggtctt cctcaccgat aaccgcttga tccagatcat
     3061 tgtcttgtta ctcatgagca cctggtggtt cggctgggca ggcacgctct tcctttcctc
     3121 cacccgcgtg atcttcgccg ccgcattcga ccgcttgctc cccgaaaagg ctgctgaatt
     3181 gaacgaccgc ggaacaccgg tcaacgcgat gctgtacatg attctgccgg cgattttggt
     3241 agcggctttg tatgcctatg atgtggctgg cttcaaatcc atcacgcttg cggcaacaca
     3301 aggcattgcc atcatgtact tcggcac
//
LOCUS       DAOQNJ010000272         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19829_length_3325_cov_7.317431, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000272 DAOQNJ010000000
VERSION     DAOQNJ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19829_length_3325_cov_7.317431"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLG52_08490"
     CDS             <1..491
                     /locus_tag="PLG52_08490"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB41525.1"
                     /translation="HVPYRGARVIQFSIEDVIDLYEHRAFLEGRAAYFAAQIITDRDL
                     TALKKIQVGMEENDAPEAVLKYRDLNRNFHKTIFTISKHKYLIRTLNQMWAAFPTMLI
                     GNFAATASHPLPARDDTDRQEHQAIMDALFAHDGTSAEQAMRNHILATREQLVSFLKA
                     NQ"
     gene            488..1294
                     /locus_tag="PLG52_08495"
     CDS             488..1294
                     /locus_tag="PLG52_08495"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNB41526.1"
                     /translation="MTLLMPIGGNENDKKPVVSKEFIRLAGGEKADIVVLPQPSVLED
                     TPEYYVQHFLSLGARSVKSLDFRSRAEAHLDEYVEVIQNATGIFISGGTQMRLPAIIG
                     GTNVETAIFEAYRRGVIVSGTSAGASVMSKVMIAYGKGGSTPREGIVRFTPGLGLTDK
                     IILDQHFRQRDRFGRLLYAVSAHPGLLGVGIDEDTAAIIEDEAKITVVGTGAVTVVDG
                     SELESTNVAEAEKGKPVAVSNLRIHVLTAGCSYHIPTRKAHIPNLQLVRE"
     gene            1344..3014
                     /locus_tag="PLG52_08500"
     CDS             1344..3014
                     /locus_tag="PLG52_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB41527.1"
                     /translation="MKHLKSVLFMMVISALLLAACGGGSAPADNTGGEATQAPGAGEG
                     NKTATFIFTQEFDNLNPLYTNQWFTAITYQLWSCYAWTYDDQNAPVPNLVTELPTTEN
                     GGISEDGRTLTFKLRDDIVWSDGEPITADDFVFTYEMTMDPANTVASQSPYDQMESVV
                     AADPQTVVVTFTEPFAPWAATLWKGIIPKHVLQPVFEADGNLNAAEYNRAPTVGCGPF
                     NFAEWEAGSFARFVANDNYWLGRPKLDEIFIRFVPDDASQIAALQAGEGDLGTFIAYS
                     DIPALEEAGVQMFTVFSGYNEGLYLNLGEKGHPALKELGVRQAIAYALDRESLVNDLL
                     LGRTGVAATYWDNTPFVDPSVAPYPYDPAKANELLDAAGWTDSNGDGTRDKDGVELVL
                     KYGTTTREIRKEIQAVFQQQLLEVGIGTELINAENFFDGYAEGGPAATGSLDIFEYST
                     TANFPDPHTSEWFCDQIPTDAAPDGSNWQAVCDTDLEALFTTESSQVDAAARETTFHK
                     ISKHVFDQAYWIGIYWDPDIWAVNARLTNVKLSGATPFYNIMEWDLAE"
     gene            3090..>3325
                     /locus_tag="PLG52_08505"
     CDS             3090..>3325
                     /locus_tag="PLG52_08505"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41528.1"
                     /translation="MTKYIVRRLLQAIPLLLLISLILFVLMQNATDPLATMGGRTPTK
                     PEDRARLTRQLGLDKPIHIQYLYWLAGNDWAKTDL"
BASE COUNT          828 a          791 c          814 g          892 t
ORIGIN      
        1 aacatgtccc ttatcgaggc gcgcgtgtca ttcagttttc tattgaagat gtaattgacc
       61 tgtatgaaca tcgtgccttt cttgaagggc gtgcagcata tttcgcagca cagattatta
      121 cagaccgtga cttaacggct ctaaaaaaga tacaggttgg aatggaagag aatgatgctc
      181 cagaagctgt attgaagtat cgtgacctta accgaaattt tcacaagact atttttacga
      241 ttagtaaaca taaatactta atccgtaccc taaatcaaat gtgggctgct ttcccaacga
      301 tgttgattgg caacttcgca gctacagcat cgcacccttt gcctgcccgc gatgatacag
      361 atcggcagga gcatcaagcg attatggatg ctttatttgc acatgacggg acttctgcgg
      421 aacaagccat gcgaaatcat attctcgcta ctcgtgagca gttggtttca ttcttaaagg
      481 cgaatcaatg acgctgttaa tgccgattgg tggaaacgaa aacgacaaga aaccagtcgt
      541 ttcgaaagag tttatccgcc ttgcaggagg ggaaaaagcc gacattgtgg tgcttccaca
      601 gccgtcggtt ttagaggata ctccagagta ttatgttcag catttcttaa gtcttggtgc
      661 tcgttcagtg aagagtttag attttcgctc acgagctgag gcgcacttgg atgaatatgt
      721 tgaggtcatt caaaatgcaa cgggtatttt tatttctggc ggcacccaaa tgcgtttgcc
      781 tgccatcatc ggtgggacaa acgttgaaac agcaatcttt gaggcatatc ggcgtggcgt
      841 cattgtttca ggaacaagtg ctggggcatc tgtaatgtct aaggtgatga ttgcttatgg
      901 aaaaggcggt tccacgccgc gcgaagggat agttcggttt acccctggct tgggattaac
      961 cgataaaatt attttggatc aacattttcg gcagcgtgat cggtttggac gtttgctcta
     1021 tgcagtttcg gcacatccgg gtttgctcgg cgtcggcatt gacgaggata cagctgcgat
     1081 cattgaggat gaggcgaaga tcacggttgt gggtacagga gcagtgaccg ttgtagatgg
     1141 aagcgaactt gaatccacca atgtggcaga agcagaaaaa ggaaaacccg ttgcggtcag
     1201 caatctaaga atccatgttt taacggcagg ttgttcatat catattccaa cacgcaaagc
     1261 acatatcccc aacctgcaat tggttcgtga gtagcatgtg aacggttcac atgttttcaa
     1321 ataacacaaa ggagaagaaa agcatgaaac atctaaagtc tgttctgttc atgatggtca
     1381 tttctgcatt gctgctagca gcgtgtggcg gtggctcagc cccggcggat aataccggtg
     1441 gcgaagcaac acaggcacct ggtgctggtg agggcaacaa gactgccacc ttcatcttca
     1501 ctcaggagtt cgataacctg aacccgctct acaccaatca gtggttcact gccattacat
     1561 atcagctctg gagctgctat gcatggacct atgatgatca aaacgcccct gttccgaatt
     1621 tggtgactga actgcctacc accgaaaatg gcggtatctc tgaagatggt cgtaccctga
     1681 ctttcaagtt gcgcgatgat atcgtatggt ctgatggtga acctatcact gctgatgatt
     1741 tcgtattcac ctacgaaatg actatggacc ctgccaacac cgtggcttct cagagtccct
     1801 atgaccagat ggaaagcgtt gtcgctgcag acccgcaaac tgtggtggtg actttcaccg
     1861 aaccgtttgc gccttgggct gcaacccttt ggaaaggcat cattccaaag catgttcttc
     1921 agcctgtgtt tgaagcagat ggaaacttga atgccgctga atacaaccgc gcaccgactg
     1981 tcggctgtgg tccattcaat tttgctgaat gggaagctgg ttcttttgcc cgttttgttg
     2041 ccaatgataa ctattggctc ggacgcccca agttggatga aatcttcatt cgcttcgtac
     2101 ccgatgatgc ttctcagatc gctgctcttc aggctggtga aggtgatctc ggtacgttca
     2161 ttgcctactc agacattccc gcccttgaag aagcaggcgt gcaaatgttc accgtcttct
     2221 ctggctataa cgaaggtttg tacctgaacc ttggcgagaa aggtcaccct gcgctgaagg
     2281 aattgggtgt tcgccaggca attgcttatg cacttgaccg cgaatccctt gtcaatgatt
     2341 tactgttggg tcgcactggc gtggctgcaa catactggga taacactccg ttcgttgacc
     2401 cgtctgtcgc cccgtatcct tatgaccctg ccaaggcgaa cgaacttctc gatgccgctg
     2461 gctggaccga ctctaatggt gacggcaccc gcgataagga cggtgttgaa ctcgtcttga
     2521 agtatggaac cactacccgt gaaatccgca aggaaattca ggctgtgttc caacagcagt
     2581 tacttgaagt aggcattggc accgaattga tcaacgccga aaacttcttc gatggctatg
     2641 ctgaaggtgg accggctgcg accggttcgt tggatatctt cgaatattcc actactgcaa
     2701 acttcccaga tcctcatact tctgaatggt tctgtgacca gatcccaacc gatgccgccc
     2761 ccgatggcag taactggcag gctgtctgcg atactgacct cgaagctctc ttcactactg
     2821 aaagcagcca ggttgatgct gccgctcgtg aaacaacctt ccataagatt tcgaagcatg
     2881 tattcgatca ggcttactgg atcggtattt actgggatcc tgatatttgg gcagtcaatg
     2941 ctcgccttac caatgtcaag ctttctggcg ccactccgtt ctataacatt atggaatggg
     3001 accttgctga ataagatttt gagttagtac taaattagca ggggttctgt aataaaattg
     3061 cagaacccct gctttttgaa ggtgaatcca tgaccaagta cattgttcgc cgtctgttgc
     3121 aagccattcc attattgctg cttattagtc tgatcctctt tgttctgatg cagaatgcga
     3181 ccgacccatt agcaacgatg ggtggtcgta cacccaccaa acctgaggat cgtgcccgcc
     3241 tcacccgcca attagggctg gataaaccca tccacattca atatctctat tggctggctg
     3301 gcaatgattg ggcaaaaacc gatct
//
LOCUS       DAOQNJ010000273         3322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19852_length_3322_cov_9.303642, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000273 DAOQNJ010000000
VERSION     DAOQNJ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19852_length_3322_cov_9.303642"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /locus_tag="PLG52_08510"
     CDS             complement(<1..1682)
                     /locus_tag="PLG52_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNB41529.1"
                     /translation="MMRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPG
                     GVIPQILEKLNVSAQALTERLDATLRASPKANIFGGGAGQIFITPRVKRIIDLANEEA
                     NRLKDEYISTEHIFLAILTERNTPAARILESAGLTRDRVYDAIQDLRGGQRVTDPQAE
                     TRYRTLEKYSRDLTQLAREGKLDPVIGRDNEIMRLIQILSRRTKNNPVLIGEAGVGKT
                     AIAEGLAQKIASNDVPEILSGKRVVALDLGAMIAGSRFRGEFEERLKAVMEEVQRAKG
                     DVILMIDELHTVVGAGAAQGAMDASNMLKPALARGELQTIGATTLDEYHKHIEKDAAL
                     ERRFAPIYVDEPSVEDTIKMLHGLRDKYEAHHKVRFSDEALSAAAHLAVRYVTDRHLP
                     DKAIDLMDEAAAKLRVALYSMPPDLKALKTEIDKLAAEEEQAGLERDYERAAQKKAER
                     LRLDQEYSGKRDQWEAEHHLDEVVDVNDIAAVVHQWTGIPVTQMLETESEKLLHMEAR
                     LHERIIGQEEAIHAVSDAIRRARSGLKDPARPIGSFIFIGPSGVGKTELAKAL"
     gene            1932..3002
                     /locus_tag="PLG52_08515"
     CDS             1932..3002
                     /locus_tag="PLG52_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41530.1"
                     /translation="MKRLFPFILTTIFILQACGGNAPTEESAAAPQTANTEVAAATAD
                     VAPATAEPIVHVDIPTEGTVSRASAHDNEESANSGNKDVIFGDDFEKNRFERPFTAND
                     MTYLPDLDIVDFSITSDDSFFYIRIDLVGLDLASQSLTGFYGVEFDKNGDGRAEIFLV
                     TQPPYKTEFTADNVFLFIDANGDVGSEQAAKPDASNGDGFDSLLFDLNDSIHPEDPDL
                     AWVRFIDDTKPAIEIAYKKWLFKDGNENFMWSVTASDELINPAEYNFHDAIAETEAGS
                     PTKSNPNYPLKGLAAIDNTCRVPFGFEAKGNEPLGCSVNNIPPEPEEEIDFCTQFPSA
                     CERLPEPPPLQQFFDKEDLKGF"
     gene            complement(3044..>3322)
                     /locus_tag="PLG52_08520"
     CDS             complement(3044..>3322)
                     /locus_tag="PLG52_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNB41531.1"
                     /translation="MPEFEAWIVDQKRKTNLNVVGTDSTGDVSLMNHTLARPVAVVLG
                     NEAKGMSVALKNLCDYVVSIPVEGAVNSLNVACAGSIILWDVYKNSHR"
BASE COUNT          826 a          931 c          743 g          822 t
ORIGIN      
        1 agagctttgg caagttcggt ctttcccaca ccggaaggtc cgataaagat aaacgaaccg
       61 atggggcgag ccgggtcttt caaaccagaa cgcgcgcggc gaatggcatc agatacggca
      121 tgaatggctt cttcctgccc aatgatgcgc tcatgcaaac gcgcttccat atgcaagagt
      181 ttttctgatt cggtttcaag catctgcgtg acaggaatgc ccgtccactg atgaaccacc
      241 gcagcaatgt catttacatc caccacttca tcaaggtgat gttcggcttc ccattgatcg
      301 cgcttgccgc tgtattcttg gtcaaggcgt aagcgttcag ccttcttttg agcggcgcgt
      361 tcgtaatcac gttccaaacc agcttgttct tcttcggcag ccagtttgtc aatttcagtc
      421 ttcaacgcct tcaaatcagg cggcatggag tacaacgcca cgcgcagttt cgcagcagct
      481 tcatccatca agtcaatggc tttgtcgggc aggtgacgat cggtcacata gcgaactgcc
      541 aagtgagccg cagcagaaag tgcttcatca gagaagcgaa ctttatgatg cgcttcatat
      601 ttgtcacgca agccatgcaa catcttgatg gtatcttcca cgctcggctc atccacatag
      661 atcggagcaa agcggcgttc gagcgccgca tctttttcga tatgtttgtg atactcatcc
      721 aacgtagtcg cgccaatggt ctgcaactcg ccacgcgcca acgccggctt gagcatgttg
      781 ctggcatcca tcgcgccttg agccgcgccc gcccccacaa ccgtatgcaa ctcatcgatc
      841 atcagaatga catcaccctt cgcacgctgg acttcttcca tcacagcctt gagacgttct
      901 tcgaactcgc ctctaaatct ggacccggca atcatcgcgc ccaaatcgag cgctaccact
      961 ctcttgccag atagaatctc gggcacatca ttgctggcaa tcttctgtgc caaaccttct
     1021 gcaatggctg tcttccccac acccgcttca ccgatcaaaa ccgggttgtt cttcgtacgg
     1081 cgagacagaa tctgaatcag gcgcatgatc tcattatcgc gcccaatgac cgggtcaagc
     1141 ttaccttcac gggcaagctg cgtcaggtca cgcgaatatt tttcaagggt gcggtaacgc
     1201 gtctccgctt gcggatcggt cacccgttga ccaccgcgca gatcctgaat ggcatcatac
     1261 acacggtcac gggttaatcc tgcagattcc aaaatgcgag ccgcaggggt attacgttca
     1321 gtgagaatcg ccaaaaagat atgttcggtt gaaatatatt catctttgag acggttggct
     1381 tcttcgttgg caaggtcgat aatccgcttg acgcgcgggg tgatgaaaat ctgtcccgct
     1441 ccgccgccga agatgttcgc cttggggctg gcgcgcaggg tggcatccag ccgttcggtg
     1501 agcgcttgag cgctgacatt caatttttca agaatttggg gaatcactcc accaggctgt
     1561 tcaatcagcg ccagcagaat gtgttcggta tcaatttgat tgtgcccata gcgttggata
     1621 atctccgcag cacgctgggc ggcttcttgt gctctctctg taaatcggtc aaatcgcatc
     1681 ataggggtat cctccggaca gaccacagac catggatcgc agaccatggt tttatggtct
     1741 atcgcccatt gtctatcgtc cacccaatgc tggcattata acaaacgccg tatcggcgaa
     1801 ttgtcagtat ggcataagaa gagtgttaga tttcggcgac catcactaaa gtaataccct
     1861 attccttact tttttgggtg tagcgccaga aaagaataaa ctattatctc caatatatcg
     1921 ttaggagacc catgaaacgc cttttcccct ttatcctcac tacaattttc attttgcaag
     1981 cctgtggcgg aaatgccccc acagaagaat cagccgccgc tccccaaact gccaatacag
     2041 aggtcgctgc tgccacagca gacgttgcac cagctactgc agaaccgatt gtacatgttg
     2101 acatcccaac cgaaggtacg gtgagccgag cctcagctca tgacaacgaa gaaagcgcca
     2161 actctggaaa taaagatgtg atctttggtg atgacttcga gaaaaatcgt tttgaacgcc
     2221 cctttacagc caacgatatg acctatctcc ccgatctcga tatcgtggat ttctccatca
     2281 ccagcgacga ctccttcttt tatatccgca tcgatctggt cggacttgat cttgccagcc
     2341 agtctctgac cgggttctac ggtgtggagt ttgacaagaa cggcgatggt cgcgccgaaa
     2401 tttttcttgt aacccaaccg ccttataaaa cagaattcac tgctgataat gtctttctct
     2461 ttattgatgc caatggagac gtaggcagcg aacaagccgc caaacccgat gcttccaacg
     2521 gcgatggttt cgactctctg ctctttgacc tgaacgatag catccacccc gaagaccccg
     2581 atctggcatg ggtccgcttc attgatgaca ccaaacccgc cattgaaatc gcctacaaga
     2641 aatggctctt caaagacggc aacgagaatt tcatgtggag cgttaccgcc agcgacgaat
     2701 taatcaatcc tgctgaatac aacttccacg atgccatcgc agaaacagaa gccggctcac
     2761 caacaaagtc caaccccaat tatcctctca aaggtcttgc cgctatagac aatacatgcc
     2821 gtgtcccatt tggtttcgaa gcgaaaggaa acgaaccctt gggatgttca gtaaacaaca
     2881 taccaccgga gcccgaagaa gaaatagact tctgtacaca gtttccatcg gcatgcgaac
     2941 gtttgccaga accgccaccc cttcaacagt tttttgataa agaagatttg aaaggatttt
     3001 aattagatag ataaaaactg cgcggacttt atccgcgcag tttttatcta tggctattct
     3061 tatatacatc ccacaaaatg atcgaccccg cacacgccac atttagcgaa ttgactgctc
     3121 cctctactgg gatgctcaca acatagtcac atagattttt gagcgccaca ctcatgccct
     3181 tggcttcatt acccaaaact accgcaacag gtcttgcaag ggtgtggttc atcagagaga
     3241 catctcccgt cgaatcggtt cccacaacat tgagattggt cttgcgcttc tggtccacta
     3301 tccacgcttc gaactccggc at
//
LOCUS       DAOQNJ010000274         3321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_19859_length_3321_cov_9.296387, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000274 DAOQNJ010000000
VERSION     DAOQNJ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19859_length_3321_cov_9.296387"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1589
                     /locus_tag="PLG52_08525"
     CDS             144..1589
                     /locus_tag="PLG52_08525"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNB41532.1"
                     /translation="MTQPTYTDFYTLIPYLILTVWACVLLIVDLFIPKGRKGITAFLA
                     ALGIAISLGYSITQTGIESTGFSSMVSLDGFSTFANILLLGSGLFAIALAYGYTKRMG
                     IERGEYYTLLLFSLTGMMLMAQANDLIMVFIALELLSIPLYVLSAISRKLTSEESGVK
                     YFLLGAFASGFVVYGTALIFGATGSTNLNVILVQAASGVTSGLLLTIGAALLLVGFGF
                     KVAAVPFHMWTPDVYQGAPTSVTAFMAAGAKIAGFVALFRVFSTAFPALSSDLTNILW
                     ALSALTMIVGNLTAISQTNIKRMLAYSAIAHAGYILMAFVPYGNAKVAPTAIAAGLFY
                     LVAYALSNFGSWGVVIALEKAEGKGLEIEDYAGLAKKYPALAVAMTVFMLSLIGFPPT
                     LGMVGKFYLFRSALAGGFPGLALIGVVTSLISAYYYLRVVVNMYMKEGDPEVEREPWL
                     GLTTALTAVVTVAVSLIPQFLFTLAYTAVMR"
     gene            complement(1634..2356)
                     /locus_tag="PLG52_08530"
     CDS             complement(1634..2356)
                     /locus_tag="PLG52_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41533.1"
                     /translation="MQIAIIFLGTLILLVLIWLLLTELRREKPDEQSEPVSENSQRVF
                     TKWLENFKTWSGERYPINIERLGFTVLWSIFFLSLLDHLQTRSGLTGTLIYFLVILPH
                     EAGHFICSPFGWFLSIAGGSIWQVLLFVLFGFIVLFRKRRNEALGWWAVAGHSLISLS
                     TYIADASARSLPLIPIPDPSRHDWGNMLEYLGLLQYDWLFAKISIITGGAIVLSCVVA
                     GLFFTWYQPQNLFNKPEIDTAK"
     gene            2472..2546
                     /locus_tag="PLG52_08535"
     tRNA            2472..2546
                     /locus_tag="PLG52_08535"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2504..2506,aa:Lys,seq:ctt)
     gene            complement(2582..>3321)
                     /locus_tag="PLG52_08540"
     CDS             complement(2582..>3321)
                     /locus_tag="PLG52_08540"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNB41534.1"
                     /translation="SLSLSMTIEKRIMTGTIINVAAILLGGTVGLLFGSRIPERFKNT
                     IIAGMGLFTVGMGLQMFLKSENQLIVLGAILIGTIFGEWLGIEERLQDFGLALEKRFS
                     SDNESGTGSRFVRGFMVASLLFCIGPMAILGSIQDGLTGDYSLLAVKSTLDGFASIAF
                     ASTMGIGVMFSSIIIFFYQGGISLLAERLSTIISDPMMNEMTATGGVLLMGVGISNLL
                     ELKKIRVGNFLPALVVAPLIVWVLGKL"
BASE COUNT          835 a          871 c          737 g          878 t
ORIGIN      
        1 ttgtacccca agcctttctt cgatatcctc gcccctgcgg ttgagaagtt gctgtccgct
       61 ttacctttgt aatagaccat ggacaataga cgactgacca taatttgttg tctgtcattc
      121 attgtcagaa atttggagcg ttcatgacgc aaccaaccta caccgacttt tacacactca
      181 tcccttatct catcctgacc gtttgggcat gtgtgttgct gattgttgac ctattcattc
      241 caaaaggtcg caaaggcatc actgcatttc ttgctgcatt gggcatcgcc atctcgcttg
      301 ggtattcaat tacgcaaaca ggtattgaaa gcaccggctt cagcagcatg gtttcgcttg
      361 acggcttctc cactttcgcc aatatccttt tgctcggaag tggactcttc gccatcgccc
      421 ttgcctacgg ctataccaaa cgcatgggca tcgaacgcgg cgagtactat actctgctgt
      481 tattcagcct caccggcatg atgctcatgg cacaagcgaa cgacctcatc atggtcttca
      541 ttgcgctcga attgctttcc atcccgctct atgtcctctc tgcgatctcc cgcaagctga
      601 cctctgaaga atctggcgtg aaatacttct tgctcggcgc cttcgctagc ggatttgtgg
      661 tctatggaac cgctctgatc ttcggtgcga cgggctctac caacctcaat gtgatcttgg
      721 ttcaagccgc ctctggcgtg acctcgggct tgctcctcac cattggggct gcgcttttgc
      781 tggtaggctt tggcttcaaa gtagccgccg ttcccttcca catgtggacc cccgatgtct
      841 accaaggtgc accaacttct gtcacagctt tcatggcagc tggtgcgaag attgctggtt
      901 ttgtagcatt gttccgcgtt ttttctactg cgttccctgc tctctcttca gacctcacca
      961 atatcctctg ggcgctctct gcgttgacca tgatcgttgg taacctcacg gcaatttcac
     1021 agaccaatat caaacgcatg ctggcatact ctgcgattgc gcatgcgggg tatatcctca
     1081 tggcgttcgt cccttatggg aatgcgaaag tcgccccaac tgcaattgca gcaggcttgt
     1141 tctatctggt agcctatgct cttagcaatt ttggttcttg gggtgtcgtg attgctcttg
     1201 aaaaagccga aggcaaaggg ctcgaaattg aagattatgc tggcttggca aagaaatatc
     1261 ccgctctcgc agtcgctatg actgtcttca tgctctccct gatcggcttc cctccaaccc
     1321 ttggtatggt ggggaagttc tacctcttcc gctctgccct cgcaggtggt ttccccggtc
     1381 ttgcccttat cggcgtggtg acctctctta tttccgcata ctactacttg cgcgttgtgg
     1441 tcaacatgta catgaaggaa ggcgaccctg aggttgaacg tgaaccttgg cttggcttaa
     1501 ccacagccct tacagcagtc gtaactgtag cagtgagcct cataccgcaa ttccttttca
     1561 cactggcata tacagctgtg atgcgataaa cataaaaaag acaaaaagag cgctgacatc
     1621 tgtcagcgct cttttatttt gcagtatcga tttctggctt gttgaataag ttttgaggct
     1681 gataccaagt aaagaaaagc cctgctacaa cacaagaaag aacaattgca ccaccggtga
     1741 taatcgaaat cttcgcaaat aaccagtcat attgtagcaa ccccaagtat tcgagcatat
     1801 ttccccaatc atgccgtgac gggtctggga tggggatgag gggcaggctg cgggcactgg
     1861 catctgcaat gtaagtggaa aggctgatca ggctatgacc tgcaactgcc caccaaccca
     1921 aagcttcatt gcgccgtttg cgaaagagga cgatgaagcc aaagagtacg aataaaagca
     1981 cctgccagat tgatccgcct gcaatgctta gaaaccaccc gaagggggag cagatgaaat
     2041 gcccagcttc gtgaggcaaa attaccagaa aatagatcaa cgttccggtc aagcctgagc
     2101 gagtttgcag atgatccaat aaagaaagga agaaaatgct ccagagtacg gtaaacccaa
     2161 gtcgctctat gttgatgggg taacgttctc cagaccatgt tttgaagttt tcaagccact
     2221 ttgtaaagac tctctggctg ttttcagaaa caggttcact ttgctcgtca ggtttttctc
     2281 ttcgtaattc ggttaaaagc aaccaaatga gaaccagaag gataagggtg cccaaaaaaa
     2341 tgatcgcaat ttgcatggaa gccttctttg attataaaag ctattttccg gcggctctcc
     2401 ttgcaaaaaa cgtacgagtt gtaattattg acacaatacc cctttgcggt ataataccgc
     2461 ccgcacgaaa tggctccgta gctcaatggc agagcagttg actcttaatc aattggttga
     2521 gggttcaaat ccccccggag tcaccagtca aaaaacatcc ccatttgggg atgttttttg
     2581 tttatagctt tcctaacacc cacacaatga gtggagcaac gaccagcgcc ggtagaaaat
     2641 tcccaacccg aatctttttg agttccagta gattactgat tcccaccccc atcaacaata
     2701 ccccacctgt ggcggtcatt tcattcatca tggggtcact gataatagtg gaaagtctct
     2761 ctgccagcaa actgatgcca ccttgataaa agaagataat gatcgaagaa aacatcacac
     2821 cgattcccat cgtggaagca aaggcaatag aggcaaaccc atccaacgtg gatttaaccg
     2881 ccagcaggct atagtcaccc gtcaacccat cttgaatgga gccaagaatt gccattggac
     2941 ctatgcaaaa tagcagggat gccaccatga agccacgcac gaaacgagag cctgtgccgc
     3001 tttcattatc gctagagaag cgcttttcta atgccaagcc aaaatcttgc aaacgttctt
     3061 caattccaag ccattcccca aagatggtgc caatcagaat ggcgcctaat acgatcaatt
     3121 gattttcgct tttgaggaac atttgcaaac ccatacctac ggtgaacagc cccatccctg
     3181 cgatgatggt gttcttaaaa cgttctggga tgcgcgaacc gaataatagt cctaccgttc
     3241 ccccgagcaa aatggcggca acattgatga tcgttccagt catgattctc ttttctattg
     3301 tcatgctaag cgacagcgaa g
//
LOCUS       DAOQNJ010000275         3295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20134_length_3295_cov_11.107407, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000275 DAOQNJ010000000
VERSION     DAOQNJ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20134_length_3295_cov_11.107407"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PLG52_08545"
     CDS             complement(<1..1253)
                     /locus_tag="PLG52_08545"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB41535.1"
                     /translation="MTSTALETTNSIETPKDMKTFFTIWVGQLISMLGSGLTNFALAV
                     WIYDQTKQATPFAITVLLGTIPRVLLLPIAGSVADRFNRRFIMIAADTCNALLTLVIF
                     LLLGAGNLQLWHIYVIAFLESVFAAFQEPAFSASISSIVPKEKLGSANGMLQMREALS
                     TVITPVLAGILFVAIGFNGIIMIDFVTFFFAVGALLLVKIPQPEQTQEHKDKNVWQDI
                     VFGWNYLRERTGLFGLILYYAMVNFLLNWSAVLLIPMVLSRFTADVLGVIQTVMGVGM
                     LVGSVIMSAWGGAKQRIPAAIGFIVLGVTGFIIAGLQPNPWLIGAGIFLLMLSVPLAS
                     GNSQVVFQTKVPREIQGRVFSVRSTISQSMMPLAFILAGPLADKFFEPLLMDGGALAN
                     TLVGQILGTGVGRGIGLMFIISGLIG"
     gene            complement(1250..1882)
                     /locus_tag="PLG52_08550"
     CDS             complement(1250..1882)
                     /locus_tag="PLG52_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB41536.1"
                     /translation="MKKDKEEKKRFHVSDLETLRAIADPLRVQILELLEGQALTVKQV
                     AEKLGLASSKLYYHFGALEKLGLIEVAETRMVANMLEKTYRSAAHLLDVDPTLFNFSK
                     GGDNESINILLSSTIDATREDLLRSLQARQFQLEQGAEEQPRRIIINRVVSSASEKRI
                     AEFQERLVELIQEFEEEDKTSKSSDQPYALTVAFYPSFYFDKTVKKGKKK"
     gene            2032..2778
                     /locus_tag="PLG52_08555"
     CDS             2032..2778
                     /locus_tag="PLG52_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNB41537.1"
                     /translation="MQALGIDIGGSGIKGAPVNIETGELLAERLRIKTPKGAKPEPVA
                     EVVAEIVRAFKWNGPIGVGFPAPIKNGVALMAANVSEKWVGTNADELLTKTTGCVCKV
                     GNDADVAGMAEMKFGAGKGQHGTVIMITLGTGIGTAIFYNGHLLPNTEFGHLQMDGKD
                     AEHRASDAARQAKDMSWKKYAKRLNEYLNEMEKLFWPDLFIVGGGISKYHEKYLPLLE
                     LQAKIVPAQFQNEAGIVGAALYSQGMDVKV"
     gene            complement(2829..>3295)
                     /locus_tag="PLG52_08560"
     CDS             complement(2829..>3295)
                     /locus_tag="PLG52_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HNB41538.1"
                     /translation="QELIDAGHDVMNIAAAAIKISRGDEKQRPIAEVAQVTDERRDRD
                     FSRGGRRETFGRSEKPGRFGDKARVKGSRTHEEGMVRLKVNKGKAHNIRPNDIVGQIA
                     FHANIPGNIIGKIRIEEKMSFVDIPEDVVEQVLKHSGNYKIGKDKFSVVKAQ"
BASE COUNT          905 a          804 c          769 g          817 t
ORIGIN      
        1 ccgatcaagc cggagataat gaacatcaaa ccaatgccgc gcccgacacc cgtcccaaga
       61 atctgaccca caagggtatt tgcaagcgca ccaccgtcca tgagtaaagg ctcaaaaaat
      121 ttatcggcaa gtggacccgc cagaataaat gcgagcggca tcatggattg tgaaatagtc
      181 gagcgaacag agaagacacg cccttgaatt tcacgtggca ctttggtttg aaaaacaacc
      241 tggctattgc cgctcgcaag cggcaccgat agcatcaaga ggaagatacc agcgccaatc
      301 aaccaagggt tgggttgtaa accagcaatg ataaaaccgg tcacaccgag aacgataaaa
      361 ccgatggcag ccggaatgcg ctgtttcgcg ccgccccatg cgctcataat aacgcttcct
      421 actaacatgc ccacgcccat cacagtttgg atcactccca acacatcggc agtgaagcgg
      481 ctcaagacca tcgggatgag taaaactgca gaccagttaa gcagaaagtt gaccattgca
      541 taataaagga tcaacccaaa cagacccgtt cgttctcgca gataattcca accgaagacg
      601 atatcttgcc aaacgttctt gtctttatgt tcctgcgttt gttctggttg tggaattttc
      661 accagcaaca acgcacccac tgcaaagaag aaggtcacaa agtcgatcat gatgatgcca
      721 ttgaaaccaa tggcaacaaa taagatacca gccaacacgg gggtgataac cgtgctcaac
      781 gcttcacgca tttgaagcat gccattggca ctgcccagtt tttctttggg aacaatactg
      841 gagatcgatg cagaaaatgc tggttcttga aaagcagcaa acaccgactc taaaaaggca
      901 attacataaa tatgccaaag ttgcaaattc ccggcgccta aaagcaagaa gatcaccaag
      961 gtcaataaag cgttgcaggt gtccgcagcg atcataataa agcgtcgatt gaagcggtct
     1021 gcgacagaac ctgcgattgg cagcaataaa acacgcggaa tggttcccaa taacacggta
     1081 atagcaaagg gagttgcttg cttggtctgg tcgtagatcc atacggcgag ggcgaagttc
     1141 gtcagcccag agccgagcat ggagattaat tgtccgaccc agatcgtgaa aaaggtcttc
     1201 atatcttttg gcgtttcaat actattcgtt gtttctaaag cggtagatgt catttctttt
     1261 tgcctttctt gacggtcttg tcaaaataaa aactcgggta gaacgctacc gttaatgcat
     1321 aaggttggtc ggaagatttt gatgttttat cttcctcttc gaattcttgg atcagttcca
     1381 ctaaacgctc ttgaaattca gcaatccgct tctcggaggc gctactcact acgcgattga
     1441 taatgatgcg gcgcggctgt tcttctgcgc cctgctccag ttggaattgg cgggcttgta
     1501 aacttcgcaa tagatcttcg cgggttgcat caatagtgga tgaaagcaaa atgttgatcg
     1561 attcattatc gccgcccttt gaaaaattaa agagagtcgg gtccacatcc aaaagatgag
     1621 cagcagaccg ataggttttc tccaacatat tggcaaccat gcgagtctct gccacttcga
     1681 tcaaccctag tttttcaagc gcgccaaagt gatagtagag cttactggat gcaagaccca
     1741 atttctctgc tacctgctta accgttagcg cctgcccttc gagcagctcg aggatttgca
     1801 cccgcaaagg gtctgcaatg gcacgcaggg tttccaagtc ggaaacatga aagcgttttt
     1861 tctcttcttt atcttttttc atagaaaaat ttcctcttat cgttaaaata ttttttaacg
     1921 ataagtctga ttttacttat tttcgggaaa gagtcaaggg ttttccccct cccccttttt
     1981 agggaagttg gcattatcat agccctcata cctcaactta ggagtcgacg catgcaagca
     2041 ttaggcattg acattggtgg ttctggcatc aaaggcgcac ccgtcaatat tgaaacagga
     2101 gaattgctcg cagaacgttt gcgcatcaaa acgcccaaag gcgcaaagcc cgaacctgta
     2161 gcagaagtcg tagcagagat cgttcgtgca ttcaagtgga acggaccgat tggcgtgggg
     2221 ttccccgccc ccatcaaaaa cggagtcgca cttatggcgg caaacgtttc agaaaaatgg
     2281 gtaggcacga atgctgatga attgctgaca aagaccaccg gttgcgtttg taaagtaggc
     2341 aacgatgccg atgtggcagg catggcagag atgaaatttg gtgcaggcaa ggggcaacat
     2401 ggcacagtga tcatgatcac actgggcaca ggcattggta ctgctatttt ttataatggg
     2461 catttactgc ccaatacgga gtttggtcac ctgcaaatgg acggcaagga tgcagaacac
     2521 cgcgcatcag atgcggcacg tcaagccaag gatatgtctt ggaaaaaata cgccaaacgc
     2581 ctgaatgaat atctgaacga gatggaaaaa cttttctggc ccgacctgtt cattgttggc
     2641 ggcggtatca gcaaatatca cgaaaaatat ttgccacttt tggaattaca agccaagatc
     2701 gttccggcac aatttcaaaa tgaagcaggt atcgtcggtg cagcgttgta ctcgcaaggt
     2761 atggacgtga aggtttagtc ttcgaatcaa aaagggacct gatagaaata tcaggtccct
     2821 ttttttattt actgcgcttt gacaacgcta aatttatctt tcccgatctt gtaatttcca
     2881 ctatgcttta gcacttgttc gaccacatct tcggggatat cgacaaaaga catcttttct
     2941 tcgatgcgga tcttaccaat gatattgcca gggatattcg catggaaggc gatctgccct
     3001 acaatgtcgt tcgggcgaat gttgtgcgcc ttgcctttgt tgaccttcaa cctcaccatc
     3061 ccctcctcgt gagtgcggct ccctttaacc cgcgctttgt ctccaaaacg cccgggcttt
     3121 tcgctgcgcc caaacgtctc gcgccgtcct ccgcgagaga aatcacggtc acggcgttcg
     3181 tcagtgactt gtgccacttc ggcaatggga cgctgctttt catcgccgcg tgagattttt
     3241 atggcagcgg cggcaatgtt catcacatcg tgaccggcat cgatcaattc ctgca
//
LOCUS       DAOQNJ010000276         3295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20136_length_3295_cov_8.859877, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000276 DAOQNJ010000000
VERSION     DAOQNJ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20136_length_3295_cov_8.859877"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLG52_08565"
     CDS             complement(<1..221)
                     /locus_tag="PLG52_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB41539.1"
                     /translation="MKIKLASVSIDDYDKALKFYTEILGFILKRDIPLGNDARWITVV
                     SPDDPNGVELVLEPNASYPAMKALKESLV"
     gene            complement(218..607)
                     /locus_tag="PLG52_08570"
     CDS             complement(218..607)
                     /locus_tag="PLG52_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB41540.1"
                     /translation="MATYTQNMLDYSEQIRNVTQYIREHINESLSREVLAEVAGFSVP
                     HFHRVFIATTGESAASYVRRIRLERAGRKLRMGAVDITEVALAAGYVSHAAFGKAFKQ
                     QFGLSPSEFRQLSCSHATQLLTRGMKK"
     gene            789..2753
                     /locus_tag="PLG52_08575"
     CDS             789..2753
                     /locus_tag="PLG52_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB41541.1"
                     /translation="MSTNPITQETSLKNRPALRWTLLGVWGLVVLYTIMVLGAVLSPQ
                     FMMFRDASMARILLGIPALLQTIIDYGVDLLLLFGFSVIAVILIVNRSDDWFAILTSL
                     FLIVFGARITNLANNIALTPGYETQGGLILALGDIGIVLFTMLFPDGKFAPKWMKFLV
                     PLLIITMFGIYLFPNTSFYWVKMRDNGYYFLVTVTWYMVGLGSGIYRYYRSSTLAQKQ
                     QIRWIFIGTMGPFVWFILFQALTYLFPALTNSEVASASFQAISRLAGIFMFLMLPLFV
                     AISIVQARLFDIDMIINRSLVYGILTIGLGLTFGLVFIIASAIFRSMHASDQFFFVAV
                     IFSVAAGAGFQPLRKKLQRFVDRFFYHIQIDYQKTPLDVRAEADTNVEGGITLSSYRN
                     LKLIGHGGMAEVYRATDSTGRGTVAVKILRAALAGDEQFQKRFMREAEIVNRLEHPNI
                     VRVTDFGEERGMYYIVMEMLSGPDLNHLIRQEKRISLSNTVNLLRGIADALDYAHENG
                     LVHRDIKPSNVMLDSSTIPSRSVLTDFGIAKMLDAHTRITNTAGMLGTFDYMAPEQIQ
                     ASADVDGRADIYALGVMTYQMLTGRLPFERPDTGAILLAHLNAPPPDARELIHDLPRS
                     AAHAVQRAMAKDVRDRFSSAKDFISALETD"
     gene            2888..>3295
                     /locus_tag="PLG52_08580"
     CDS             2888..>3295
                     /locus_tag="PLG52_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41542.1"
                     /translation="MSKDTVRQIANALSVVIALAVNILASTLPLNGQNTGEISDRFLV
                     YFVPAGYVFAIWGIIYIGWIAFTIYQFQPAQKENPRLQNLGYGFAVSGLFNAAWLVCW
                     HYNWFGLSVLVMLTLLALLIASYLKLNVGLIPVN"
BASE COUNT          787 a          780 c          761 g          967 t
ORIGIN      
        1 ctaacaagtg attctttaag agctttcatg gcaggataac tagcattcgg ctcaaggact
       61 aactccacgc cattgggatc atctggtgag actacggtaa tccaacgggc atcattccca
      121 aggggaatat cccgctttaa gataaaacct aaaatctctg tatagaactt tagagcctta
      181 tcataatcat caatcgatac acttgccaat ttgatcttca ttttttcatt cctctcgtta
      241 atagttgtgt ggcatggcta caactcaact gacggaattc actaggactc agaccgaact
      301 gttgtttgaa agctttccca aatgccgcat gagagacata ccctgctgcc aatgcaactt
      361 cggtgatatc cacggcgccc atgcgaagtt ttcgccccgc ccgttcgagc cgaatgcggc
      421 ggacataact tgctgcactc tctcccgtgg tggcaataaa tacgcggtga aaatgcggca
      481 cagagaaccc tgccacctct gccaatactt cacggctcag ggattcatta atatgctcac
      541 ggatatattg ggtaacgttt cgaatctgct cagaataatc caacatgttt tgagtgtagg
      601 tcgccatgcg gctcaatata tttcagagaa agagggtctc gctactccaa atgtatcatg
      661 aaccttagaa aaaataggca acttttcgcc tgctacagcg taagttgact aaaccccgac
      721 caccttccaa aaataaaaag agtgtgaagc ggttttggag ttatgatttt gaaaatcaac
      781 ggcaacttat gtccacgaac cctatcacac aagaaacctc tctgaaaaat cgtcccgcac
      841 tgcgttggac gctgcttggg gtttgggggc tggttgtgct ctacactatt atggtgttgg
      901 gggcagtcct ttcgccacag tttatgatgt ttagagatgc gagcatggct cgcattttgc
      961 ttggcatccc tgcgcttttg caaaccatta tcgactacgg cgtagacttg cttcttttat
     1021 ttggattctc ggtgattgct gtgattctga tcgtcaaccg ctccgatgac tggttcgcga
     1081 tcctgacctc ccttttcttg atcgtcttcg gcgctcgtat caccaacctt gcaaacaata
     1141 ttgcgttaac tcccggttac gagacgcagg gcgggctgat cttggcgttg ggggatatcg
     1201 gcattgtgct gtttacgatg cttttcccag atggaaagtt cgctcccaaa tggatgaagt
     1261 ttcttgttcc gcttctgatc attaccatgt ttggaattta tctttttcca aatacatctt
     1321 tctactgggt aaagatgcgt gacaatggct attactttct ggtaactgtc acttggtata
     1381 tggttgggtt aggaagtgga atctatcggt attatcgctc ttctaccttg gcgcagaaac
     1441 agcagattcg ttggatcttt atcggcacga tgggaccatt tgtatggttc atcctgtttc
     1501 aagcgctaac atatctcttc cctgcactga caaattcaga agttgcttct gccagcttcc
     1561 aagccatttc gcgtttggct ggcattttta tgttcttaat gttgccgctt tttgtggcta
     1621 tttccattgt acaagcgcgg ctctttgata ttgatatgat cattaaccgc tcgttggtgt
     1681 atgggattct caccatcggg ctgggactca cctttgggtt ggtcttcatt atcgcctcag
     1741 caatcttccg cagcatgcat gccagtgacc aattcttttt cgtggctgtg atcttttctg
     1801 tggctgcggg cgcgggcttt cagcctttac gtaaaaaact tcagcgcttt gtcgaccgct
     1861 tcttttatca tattcaaatt gactatcaga agactccact tgatgtccgc gctgaggcag
     1921 atacaaatgt agaaggcgga attactttat cttcatatcg taatctaaag ctgattggtc
     1981 acggcgggat ggcagaggtc taccgcgcca cagactcaac cgggcgtgga accgtagcgg
     2041 tcaaaatcct gcgagccgca ttggcgggtg atgaacaatt tcagaaacgg ttcatgcgtg
     2101 aggcggagat cgtcaacaga cttgagcatc caaatattgt gcgagtgacc gattttggcg
     2161 aagaacgcgg tatgtactat atcgtgatgg aaatgctttc gggtccagac ctcaatcatc
     2221 tcatccgaca agagaagcgc atttctttat ccaacacggt aaacttgctg cgcggaattg
     2281 ccgatgcatt ggactatgcc cacgagaatg gattggttca tcgtgatatc aagccgtcga
     2341 acgtaatgtt ggattcatct actatccctt cgcgctccgt gttgaccgat tttggtattg
     2401 ccaaaatgct cgacgcccac acccgcatta ccaacacagc ggggatgctt gggacctttg
     2461 attacatggc gccggaacaa attcaagctt ctgccgatgt agatggacgt gccgatattt
     2521 atgctttggg tgtgatgacc taccaaatgt tgaccggacg tttaccgttc gaacgacctg
     2581 atacgggagc tattttgctg gcacatttga atgctccgcc gcccgatgcg cgcgaattga
     2641 ttcatgacct gccgcgctcg gcagcccatg cggtgcaaag agcgatggcg aaagatgttc
     2701 gagatcgctt tagctcggca aaagatttca tcagcgcact tgaaaccgat tgagaaaaat
     2761 aaaacacgct tatatagcgt gttttatttt tctctttttc ttctctgata gcatttgata
     2821 gtaagggaat atgaggtaat cctatccatg gtcatgaaac agtcacgaat taattatgga
     2881 gagagatatg tctaaagata cagttcgcca aattgccaat gcgctttcgg ttgttatcgc
     2941 gcttgcagtc aatattcttg ctagcaccct gccgctcaac ggacagaaca ccggtgaaat
     3001 ttcagatcgt ttccttgtat atttcgtccc tgccggatat gttttcgcca tttggggcat
     3061 catctatatc gggtggatcg ccttcaccat ttatcaattc caaccggcgc aaaaagaaaa
     3121 ccctcgcttg caaaacctgg gctatggctt tgctgtaagc ggacttttca atgccgcatg
     3181 gctggtgtgt tggcattaca actggttcgg cttgagtgtg ttggtgatgc tcactttgct
     3241 tgccctgctc atcgccagtt atctcaaact taatgtaggg cttatacctg tcaat
//
LOCUS       DAOQNJ010000277         3277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20330_length_3277_cov_9.177219, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000277 DAOQNJ010000000
VERSION     DAOQNJ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20330_length_3277_cov_9.177219"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /gene="cydB"
                     /locus_tag="PLG52_08585"
     CDS             complement(<1..982)
                     /gene="cydB"
                     /locus_tag="PLG52_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNB41543.1"
                     /translation="MSYEFLQILWFILIAVLWIGFHFLEGFDFGVGMLLPFLGKKDEE
                     RRAIINTVGSVWDGNEVWLLTAGGATFAAFPHWYATMFSGFYLALFLLLVGLILRGIA
                     FEYRSKDSSPKWRNTFDWMIAIGSFLSALLLGAAFANLARGVPINENMMFTGNLFTLL
                     NPYGLLGGVTTVTIFLLHGANFLGLKLEGELRERVNGFAKKIWVAASVLYIILGIFTY
                     IAGFWARGVVNPGFVPFAAVVVLLATGYFINNKMEGWAFIMTALNIVLTQVTFFTMTF
                     PNVMTSSTNPAFSLTIYNASSSQYTLTVMSIVALIFVPIVLVYQGWTYYQF"
     gene            complement(992..2392)
                     /locus_tag="PLG52_08590"
     CDS             complement(992..2392)
                     /locus_tag="PLG52_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNB41544.1"
                     /translation="MDPITLSRWQFGLTTVYHFLFVPLTLGLSWFVAYFQTRYYQTKD
                     ETYRKMTKFWGKLFLINFAIGVVTGIVQEFQFGMNWSEYSRFVGDIFGAPLAVEALMA
                     FFLESTFLGVWIFGEGKVSEKAHLTSIWLAAIGSNLSALWILLANGWMQHPVGFVINE
                     VTGRAELVDFFALITNPKGWLFFWHTISAGLTTASFLILGVSAYHLIRKQNVDFFKRS
                     FQTATIVGLVASTLVFFGGHTNGQYMFETQPMKFSSIEAHWETSQPADFSILTIGDLS
                     GKREVWSFRTSQIGIPGVLSFLACNNFNCEVRGVNDIQAEYEATYGPGDYIPLMVVTY
                     WTFRFMMSIGFLMMLLSFLFLLALRGNFENAKWMKWVPYVLVLPYIANMSGWILTEMG
                     RQPWIVQGLLKVQDAVSPNLTTVDLLISLIGYVVVYGSLAGAMVYLMRKYAIAGPDAA
                     MHESVDVAPALVGADD"
     gene            complement(2638..2763)
                     /locus_tag="PLG52_08595"
     CDS             complement(2638..2763)
                     /locus_tag="PLG52_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41545.1"
                     /translation="MSSQEFSKAEKFWMNFKLYQLWRFVVLNVKILMTVYQSKRS"
     gene            complement(2774..>3277)
                     /locus_tag="PLG52_08600"
     CDS             complement(2774..>3277)
                     /locus_tag="PLG52_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41546.1"
                     /translation="PPIRTYLPQALPLPLPGRYPTYKNVFAAGIAFAPPGPISKPFTN
                     KNSLLIAPAPPRTGMVSGVIGRVVALNIADLIEKGEMTHSEPMTEMVAACIASMGDSL
                     WDGNAVSIVMKPVVPDRKKYANEFGRDLFTSHLEMGVSGAWMKFMLHHTFIWKFKARP
                     GWTIIPE"
BASE COUNT          858 a          739 c          983 g          697 t
ORIGIN      
        1 ggaactggta gtaggtccag ccttggtaca cgagcacgat cggcacgaag atgagcgcca
       61 caatagacat gacagtcaat gtgtactgcg aagaggaggc attgtagatg gtcaatgaaa
      121 aggcagggtt tgtgctggag gtcatcacgt tcgggaaggt catggtgaag aaggtgacct
      181 gagtgagcac gatattgagg gcagtcataa tgaacgccca gccttccatc ttgttattga
      241 tgaaatagcc agtggcgagc agaacgacaa ctgcagcgaa cggtacgaag ccggggttca
      301 caacgccgcg agcccagaag ccagcgatgt aagtgaagat gccgaggatg atatacaaca
      361 cagaagctgc aacccaaatt ttcttggcaa agccattcac gcgttcgcgg agttcgcctt
      421 caagtttgag acccaagaag tttgcgccgt gaagcaggaa gatggttacc gtggtcacgc
      481 cgccgagcaa accgtagggg ttgagcaagg tgaagaggtt gccggtgaac atcatgtttt
      541 cgttgatggg tacgccgcga gcaaggttgg cgaaggcagc gccgagcagc aaggctgaaa
      601 ggaaggagcc gatggcgatc atccagtcga aggtgttgcg ccacttgggg ctggagtctt
      661 tggagcgata ttcaaaagcg ataccgcgca agatcaagcc gaccaacaag aggaacaggg
      721 cgaggtaaaa gccgctgaac atggtggcat accagtgggg gaaggctgcg aatgtcgcgc
      781 cgcctgcagt gagaagccag acttcgttgc cgtcccacac agagccaacg gtgttgatga
      841 tggcgcggcg ttcttcatcc tttttgccga ggaaaggcaa caacataccc acgccgaagt
      901 cgaagccttc gaggaagtgg aagccgatcc acagaacggc gatcagaatg aaccagagga
      961 tttgtaaaaa ttcgtaagac atggtgttct cctagtcatc cgcgccgacg agggcgggag
     1021 cgacatcaac ggattcgtgc atggcggcat cgggtcccgc gatggcatat ttgcgcatca
     1081 agtagaccat agcgcccgcg agagaaccgt acacaacaac ataaccgatc aacgagatga
     1141 gcaggtccac tgtggtgagg ttgggggaga cggcatcctg cactttgagc aagccttgaa
     1201 cgatccacgg ttggcgtccc atttcggtca ggatccatcc gctcatgttg gcgatgtagg
     1261 gcagaacgag cacgtacgga acccacttca tccacttggc attttcaaag ttaccgcgta
     1321 gagccagcaa gaagaggaag gaaagtaaca tcatcaagaa gccgatgctc atcatgaagc
     1381 ggaaggtcca gtaggtgacg accatcaagg ggatgtaatc gccgggaccg taggtggctt
     1441 cgtattcagc ctggatgtcg ttcacgcctc gcacttcgca gttgaagttg ttacaagcca
     1501 ggaagcttaa tacgccgggg atgccgatct gactcgtgcg gaacgaccag acttcacgct
     1561 tgccgctcaa atcgccaatg gtgaggatgg agaaatcggc aggctgtgag gtttcccagt
     1621 gcgcttcaat ggaggagaat ttcatcggct gggtttcgaa catgtactgt ccgttcgtat
     1681 gaccaccgaa gaagaccaag gtgctggcaa cgagacccac gatagtggcg gtctggaagg
     1741 agcgcttgaa gaaatccacg ttctgtttgc ggatgaggtg ataggcactc acaccgagaa
     1801 tgaggaagct ggcggtggtg agaccggcag agatggtgtg ccagaagaac aaccagcctt
     1861 tggggttggt gatgagggcg aagaaatcga ccagttcagc gcgacccgtt acttcgttga
     1921 tcacgaagcc gaccgggtgc tgcatccagc cgttggcgag cagaatccaa agggcagaga
     1981 ggttcgagcc gatcgcggca agccagatcg aggtaagatg cgctttttcc gaaactttgc
     2041 cttcgccgaa gatccagacg ccgaggaagg tcgattcgag gaagaacgcc atgagcgctt
     2101 ccactgcgag cggagcgccg aaaatatcgc cgacgaagcg ggaatattca gaccagttca
     2161 taccgaactg aaattcctgc acaatgccgg tgactacgcc aatggcaaag ttgatgagga
     2221 agagttttcc ccagaacttg gtcattttgc ggtaggtctc atccttggtt tggtagtagc
     2281 gggtttggaa gtaggctaca aaccaggaaa gcccgagggt gagggggacg aagaggaagt
     2341 ggtaaacggt tgtgagaccg aattgccacc gtgagagagt gattggatcc atagatgtgt
     2401 tctccaaaaa aataatcggc taactttatc caaattggtc gaaaccttga ctactttggt
     2461 caacgattac cattattgta aacattaaac ttatgaggtg ggcgtgaata atgtcacaaa
     2521 catggcttga ctattgtaat gttattattt tgttatgtaa tttatttgtt gcacgttttg
     2581 ggcaaaatta aaagcaccaa accaaggctt ggcgctttta acaagagggg agaatgttta
     2641 agagcgtttg ctctgataaa ccgtcattaa gattttgacg tttaagacca caaaacgcca
     2701 aagttgatag agtttgaagt tcatccaaaa cttttcggct tttgagaatt cttgtgaaga
     2761 catggcaagg ctcctattca gggatgatgg tccagccggg gcgggcttta aacttccaga
     2821 tgaaggtgtg gtgcagcata aacttcatcc acgcgccgct gacgcccatt tcgaggtgag
     2881 aggtgaacag gtcacgaccg aattcgttgg cgtatttttt gcggtctggc acaacgggtt
     2941 tcatcacaat ggagacggcg ttgccatccc agagcgagtc gcccatgctg gcaatacagg
     3001 cggcgaccat ttcggtcata ggttcgctgt gggtcatttc gcctttttcg attaagtctg
     3061 cgatgttgag cgctacgaca cgtccgatca caccgctgac catgccggtg cgcggtgggg
     3121 caggggcaat gagcagactg ttcttgttgg tgaacggttt ggagatcggc ccgggagggg
     3181 caaaggcaat gcctgcggca aatacgttct tataggtggg atatcggccc gggaggggca
     3241 aaggcaatgc ctgcggcaaa tacgttctta taggtgg
//
LOCUS       DAOQNJ010000278         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20350_length_3275_cov_10.939130, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000278 DAOQNJ010000000
VERSION     DAOQNJ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20350_length_3275_cov_10.939130"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="PLG52_08605"
     CDS             <1..1422
                     /locus_tag="PLG52_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNB41547.1"
                     /translation="RIISSSELDSLEKADYDTVEGFRKAAWDAYLSPITDERNQVMDM
                     LEEIAPTSNHVAELRLLRDRLATLPSYSRRDIHAIVHESLRLLRDENHPSKQVLIQWK
                     HANDAVNVERYGSHLYSGTAMKVDEIKPKYSDTSPTMFGFEVVNAAFDAALAREPRLI
                     AFGEDVGKLGDVNQGFKGMQEKYGEFRVTDTGIREATILGQAIGMSMRGLRPIAEIQY
                     LDYVLYALQIMSDDLSTLHWRTAGGQKAPVIVRTRGHRLEGVWHAGSPMSGILSLTRG
                     MYVLVPRDMTRAAGFYNTLLKADEPAIVVEVLNGYRVKERLPDNIGEITLPLGVPEII
                     REGKDVTIVTYGACCRIVMDAADKLSKVGINVEVIDVQSLMPFDIQGKIVESLKKTNR
                     ILFIDEDVPGGTTAYMMQEVIEKQGGYFHLDSPARTLSAKAHRPSYGSDGDYWSKPNA
                     ETVFDAVYEMMHEVDPLAYPLFG"
     gene            1459..1845
                     /locus_tag="PLG52_08610"
     CDS             1459..1845
                     /locus_tag="PLG52_08610"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNB41548.1"
                     /translation="MEKEKVSARNYRELIVWQDAIKIAKAVYQLTGKFPKQEMYALAD
                     QLRRASVSVPSNIAEGQARKSPGDFRRFLHIALGSLAEVDTQLVLAQEFGYLSKEDVD
                     PMDEQIQNLRKKLYALINTLPPTTNR"
     assembly_gap    1908..2007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2008..2454
                     /locus_tag="PLG52_08615"
     CDS             <2008..2454
                     /locus_tag="PLG52_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNB41549.1"
                     /translation="NSSWTDEGLLIHKNVNLGMAVSLGEDGLIVPVIKKAEDLSLLAM
                     ARAVNDLANRARTKKLQPDDVKGGTFTLTNHGVSGSLFAFPVINQPQAGILGVGAMQK
                     RVVVIDDAIAIRPMVYMSFVFDHRILDGASADWFLAKVKETLENWA"
     gene            2501..2827
                     /locus_tag="PLG52_08620"
     CDS             2501..2827
                     /locus_tag="PLG52_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41550.1"
                     /translation="MKSYVGKLEAYYEQGWEGMIVFSFMPDGCNGLSDLIALQDGQHL
                     SIYETDDSILWSGKIKFVKRGFFDKHKLTADIWSDSKQKGVSYADWMDWFWRKPPLKA
                     TLEIKD"
     gene            2830..>3275
                     /locus_tag="PLG52_08625"
     CDS             2830..>3275
                     /locus_tag="PLG52_08625"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNB41551.1"
                     /translation="MSDKQHDKPAINKYVHPPVVAMFFIILALLLGFFVPALAGMSQL
                     IKNIGLGLTFIGFLFGVGAFLEFRKARTTLDPHGSVKTVVTGGIYRFSRNPIYVGFLF
                     MVIGFPLAYGSLWGLVVSPMFMFALTRLVIEKEEAYLEKKFKEGYT"
BASE COUNT          825 a          775 c          820 g          755 t
ORIGIN      
        1 cgcatcattt cttcgtccga gcttgattct ctcgaaaaag cagactacga cactgtagaa
       61 ggattccgca aagccgcatg ggatgcgtat ctctccccca tcaccgatga gcgcaatcaa
      121 gtcatggaca tgctcgaaga gatcgcgccc acgtcgaacc atgtcgcaga gttgcgtctg
      181 ctccgagatc ggctggcaac tctcccgtcg tattcgaggc gcgatatcca cgctatcgtg
      241 catgaatccc tgcgcctcct gcgagacgag aatcatccca gcaaacaagt cctcatccaa
      301 tggaaacatg cgaacgatgc agtgaatgtg gaacgctacg ggtcgcattt gtattcgggc
      361 accgccatga aagtggacga gatcaagccc aagtattccg acacatcgcc aaccatgttt
      421 ggctttgaag tggtcaatgc cgcctttgat gccgcgctgg cacgtgagcc gcgcctgatc
      481 gcattcggcg aagacgtggg caagcttggc gatgtgaacc aaggcttcaa aggtatgcag
      541 gaaaaatacg gcgagttccg cgtgaccgat accggcatcc gtgaagcgac cattctcggt
      601 caagccatcg gcatgtccat gcgcggactg cgccccatcg cagaaattca atacctcgat
      661 tacgtcttgt atgccttgca gattatgagc gatgatctct cgaccttgca ctggcgcacc
      721 gctggtgggc agaaagcgcc tgttattgtt agaactcgcg gacacagact cgaaggggtt
      781 tggcatgcag ggtcgccgat gtctggcatt ttgagtctca cacgcggcat gtatgttttg
      841 gttccgcgtg acatgacccg cgccgctggc ttttacaaca ccctgctgaa agccgacgaa
      901 cccgccatcg tggtcgaggt gttgaacggc tatcgcgtga aagaacgcct gccagataac
      961 atcggcgaga tcacattgcc gcttggcgtg cctgagatta tccgcgaggg gaaggatgta
     1021 accatcgtca cctatggcgc ttgctgccgc atcgtcatgg atgccgccga taaacttagc
     1081 aaagtcggaa ttaacgttga agtcatcgac gtccaatcgt tgatgccatt cgatattcaa
     1141 ggcaagatcg tcgaatcact caagaaaacc aaccgcatct tattcatcga tgaagatgtc
     1201 cccggcggta cgacagccta catgatgcaa gaagtcatcg agaaacaagg cggctatttc
     1261 catttagact cacccgcccg caccttatct gccaaagcac accgtccatc gtatggaagt
     1321 gatggcgatt actggagcaa gccgaatgct gagacagtgt ttgatgctgt ttatgagatg
     1381 atgcatgagg tcgatccatt agcgtatccg ctttttggtt gacggctggt ggttgatagt
     1441 tggcagtggt gaggttttat ggaaaaagaa aaggtttcgg caaggaatta tcgagagttg
     1501 attgtttggc aagatgctat taagatcgca aaggctgtct atcaattgac tgggaaattc
     1561 ccaaagcaag aaatgtacgc tttggcagat caactccgtc gcgcttcagt ctcggttcca
     1621 tcaaatatag ctgaaggtca ggcaaggaaa tcacctggag acttccgaag gtttttacac
     1681 attgcacttg gctctcttgc agaagtagat acgcaactgg tcttggctca ggaatttggg
     1741 tatttgagca aagaggatgt tgacccaatg gatgagcaaa tacaaaactt gcgcaagaaa
     1801 ctttacgcac tcatcaacac actgccacca actaccaacc gataaccacc aactggagat
     1861 cttatggcaa ccaaagttta tgtaccccgg ctcggcgaag gcgtggannn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnaat tcttcatgga cagatgaagg cttgctgatt
     2041 cataagaatg tcaatctcgg aatggcggtt tcgctcggtg aagatggctt gatcgtgcca
     2101 gttatcaaga aggcagaaga tttgtccctg ttagcaatgg ctcgtgcagt caatgacctt
     2161 gcaaatcgtg cccgcacgaa aaagttacaa cctgatgacg taaaaggtgg cacattcaca
     2221 ctcaccaacc acggggtaag cggatcactc ttcgcgttcc cggtcatcaa ccagccccag
     2281 gcaggcatcc tcggcgtggg tgcgatgcaa aagcgagtgg tcgtcatcga cgatgcaata
     2341 gctatccgcc caatggtata tatgtccttc gtcttcgatc accgcatctt ggacggtgca
     2401 tcagcagact ggttcctggc aaaggtcaaa gagacgttgg agaattgggc ttgaggaagt
     2461 gacgagtaaa gagaaatgag tgataagtag cgagtaacaa gtgaagagtt atgttggcaa
     2521 gttggaagcc tattacgaac aaggctggga aggcatgatc gttttcagct ttatgcccga
     2581 tggctgcaat gggctgagcg accttatcgc cttgcaagac ggtcaacatc tgagcatcta
     2641 cgaaaccgac gacagtatcc tctggtctgg aaagataaaa tttgttaagc gcggcttttt
     2701 cgataaacac aaactcacag cggatatctg gtcagactca aagcaaaaag gcgtctccta
     2761 cgccgattgg atggattggt tctggcgcaa accgccgttg aaagcaacat tagaaataaa
     2821 ggattgaaaa tgagcgataa acaacatgac aaaccagcaa tcaacaaata tgtccacccg
     2881 cctgtggtgg caatgttctt tatcatcctt gccctgctct tgggattttt cgtcccggca
     2941 ttggctggca tgtcgcaact gataaaaaat atcgggttgg gattaacctt catcggtttc
     3001 ctttttggtg tgggtgcatt tttagaattt cgcaaagctc gcacaaccct cgacccgcat
     3061 ggctcggtca aaactgtagt gacaggcggt atttatcgtt tctcgcgcaa cccgatctat
     3121 gtagggtttc tcttcatggt gatcggcttt ccgcttgcat atgggtcact gtggggcttg
     3181 gtcgtatcgc ccatgttcat gttcgcgctc acacggcttg tcatagagaa agaagaagcc
     3241 tacttggaga aaaaattcaa agagggctac accag
//
LOCUS       DAOQNJ010000279         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20374_length_3273_cov_8.530143, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000279 DAOQNJ010000000
VERSION     DAOQNJ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20374_length_3273_cov_8.530143"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2335
                     /locus_tag="PLG52_08630"
     CDS             <1..2335
                     /locus_tag="PLG52_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB41552.1"
                     /translation="PIISSSQKLGTISIQSEKAFAFSKSAINLLNELGQQAAIAIENT
                     RLYDAAQQELKERFLAEAALRSSEERYRSVSEDIPAMICRFDRGGTLTYVNQAYADFF
                     GGTPSELINKSFFDMLDEEQKDLVRSNFYSLTPDKPFIVYEIHEKNCKGEKRWVQWTD
                     RLITNENDIKAEYQSIGMDITDRKLAEIEREHLLEAEHDQRLRAETSAEATLALVSHV
                     ELDKVLIEILNQVQRLLPGCGANIALLENNILRTAAWRGYENRGEEVFKNLVKDTGMY
                     PLDQTMIKNPKTSIIADTALDENWITIPGLEWIRSHLCIPLLWNKQLLGLLYIDENTP
                     NKFSEETIHRLTPLVNATTVALESALLIETTRQALKETSALYHINKGMVALDPDELLN
                     DAVNLLKNNFDYYHVQVYVPNPATGNFYTKAASGAVGKILVEKKHEIRAGSGIVGYAA
                     ETGTPFFTNNVDGVMFYLANPLLPDTKSEMAIPVLNGDKLYGILDIQQTSARQFTQRD
                     QQLVLTVADQLAVALHKTELYENLQTALQQEKAVRNQLVQNERLAVMGRLLASVSHEL
                     NNPLQAIQNALFLLKEEKGLSQQGLNDLEIVLAESERMASLIERLRETYRPPQVEDLQ
                     PAHVNNIIEDVHALLATHLRKNSVAFEFHPDPDIPAIMALPGQIRQVTLNLFMNAVEA
                     MTDGGKLTVSTMGLNETQEIMIAVSDTGPGIAPSILPNIFDPFVTNKKRGTGIGLTIS
                     HDIVIKHRGRITAENNPETGATFKVWLPIEPASAELE"
     gene            2332..3135
                     /locus_tag="PLG52_08635"
     CDS             2332..3135
                     /locus_tag="PLG52_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB41553.1"
                     /translation="MNITGHILIIDDEASIRKTLARILQQAGFEVTTAEGAEQGLDYL
                     RTTQFDLILTDLRMPGMHGMEALKVIHTEHPTLPVILFTAQPDVNSAVEALRHGATDY
                     LLKPIKPEAIIERARNILEKQQQEKRRREIMLQIASLQNELKELDGGEPGKTQPIAPV
                     SFEKERFLKRGKLVLDLHTRRVLLNDEPLNTPPTSFDYLLVLARHAPDVVDYQTLVAE
                     AQGYQTDLREAQELTKWHIHQLRQIIEADARNPVHLINVRGTGYRLVAD"
BASE COUNT          989 a          812 c          718 g          754 t
ORIGIN      
        1 accaattatt agcagtagcc aaaagcttgg cactattagc atacagagcg agaaggcatt
       61 tgctttctct aaaagcgcca tcaacttgct caacgaactt gggcaacaag ccgccatcgc
      121 gatcgaaaat accagacttt acgatgctgc ccaacaggaa ctaaaagaac gtttccttgc
      181 agaagcagcc ttacgcagca gtgaagaacg ctatcgcagt gtttctgaag atatccctgc
      241 gatgatctgt cgctttgacc gaggtggtac gctgacctat gtcaaccaag cctatgcaga
      301 tttcttcggt ggcacaccaa gtgaactcat caacaagagt ttcttcgata tgttagacga
      361 agaacaaaaa gatcttgtcc gatccaattt ttattctctc acacctgata aaccttttat
      421 agtgtatgag atccacgaga aaaattgcaa aggtgaaaaa cgctgggtgc agtggacaga
      481 ccgattaatt acaaacgaaa atgacatcaa agccgaatat cagtcgatcg gtatggatat
      541 tactgaccga aagctggcag aaatcgagcg cgaacacctt ttagaagcag agcatgacca
      601 acgcttacgc gccgaaacca gcgccgaagc gacattggcg ctcgtatcgc atgtagagtt
      661 agataaagtt ctaatcgaga ttttgaatca ggtacaaaga cttttgccgg gctgtggtgc
      721 aaatattgcc cttctagaaa ataacatact gagaactgca gcatggcgcg gctacgaaaa
      781 ccgaggcgaa gaagtattca aaaacttagt caaagataca ggcatgtatc ctttagatca
      841 aaccatgatc aagaatccaa agacatcgat tattgccgat acagccttgg atgaaaattg
      901 gataaccatt ccggggctgg aatggatccg gtcccatctt tgcattccac tgttgtggaa
      961 taaacaatta ttaggtttgt tatacatcga tgaaaatacg ccgaataagt tttcagagga
     1021 gacgattcac cgtcttacgc ctttggtcaa tgcgaccacg gttgcgcttg aaagcgcttt
     1081 gcttatcgaa acgacccgtc aggcactaaa agagacaagt gctttgtatc acatcaataa
     1141 aggcatggta gcacttgacc ctgatgagtt gctaaatgac gccgtaaacc tcttaaaaaa
     1201 taactttgat tattaccatg ttcaggtgta tgtgcccaac cctgcaacgg gaaactttta
     1261 tacaaaagct gcaagcggag cggttggcaa gattctggtc gaaaagaaac atgagatccg
     1321 cgctggctct ggcattgtgg gctatgccgc cgaaacgggt acgcccttct tcacaaacaa
     1381 cgtagatggt gtcatgttct accttgccaa ccccctcttg ccagacacca aatctgaaat
     1441 ggcaatccct gtactcaatg gcgacaagtt atatggcatt ttggatatcc aacaaacatc
     1501 tgcacgtcaa ttcacacaac gcgaccaaca acttgtattg accgtagcag accaacttgc
     1561 agtcgcatta cataaaacgg aactatacga aaacctgcaa acagcattgc agcaagaaaa
     1621 agccgttcgc aatcagcttg tacaaaatga acgcctcgcc gtgatgggac ggttgcttgc
     1681 ctccgtttct catgaattga ataacccact gcaagctata caaaatgccc tattcctcct
     1741 aaaagaggaa aagggacttt cacaacaagg tttaaatgac cttgaaatcg tattggctga
     1801 atcagaacgc atggcaagcc tgatcgaacg ccttagggaa acctatcgtc cgcctcaagt
     1861 ggaagactta cagccagcgc atgtcaacaa tattattgaa gatgtacatg ccttgcttgc
     1921 tacacaccta cgcaagaaca gtgtggcatt tgagtttcac ccagacccag atattccagc
     1981 tatcatggca ctgccaggac agatacggca agttacatta aatcttttca tgaatgctgt
     2041 agaggcaatg acagatggtg gcaaacttac agtcagcacg atgggtttaa atgagacaca
     2101 agagatcatg atcgctgtat ctgatactgg tcctggtatc gcgccaagca tcctgccaaa
     2161 catttttgac cccttcgtta caaacaaaaa acgcggcaca ggcattgggc ttacgatctc
     2221 acacgatatt gttatcaagc acagaggacg tatcaccgcc gagaacaacc ccgaaactgg
     2281 cgcgaccttc aaagtttggt taccaattga acctgcctca gcggagttag aatgaacatc
     2341 accggtcata ttctcatcat tgatgatgaa gcatcgatcc gcaaaacctt ggctcgcatc
     2401 ttgcaacaag ccgggtttga agtgaccact gctgaaggcg ccgaacaagg gcttgactat
     2461 ctgcgcacca cacaatttga cttgatccta accgacctac gcatgccagg aatgcacggc
     2521 atggaagctc tcaaggtcat ccacacagaa cacccaacgt tgccggttat tctcttcacg
     2581 gctcagcccg atgtgaactc ggcggtcgaa gctttgcgtc atggtgctac agattacctg
     2641 ctaaaaccca tcaaacctga agccatcatt gagcgagcac gcaatatctt agaaaagcaa
     2701 caacaagaaa agcggcgcag agaaattatg ctccaaattg cttccttgca aaatgagtta
     2761 aaagaattag atggcggcga accgggcaaa acccaaccca tagcgccggt ttcttttgag
     2821 aaggaacgtt tcctcaaacg cggcaaactg gtgttagacc tacacacccg ccgtgtgcta
     2881 cttaacgatg agccgctcaa caccccaccc acctctttcg attatttact tgtcctcgct
     2941 cgccatgcgc cagatgtggt cgattaccag acccttgttg ctgaggcaca gggctatcaa
     3001 accgacctac gcgaagcgca agaactcacc aaatggcaca ttcatcaatt gcgccagatc
     3061 attgaagccg atgcgcgcaa cccggtacat ttaatcaatg ttcgcgggac ggggtaccga
     3121 ctggttgcag attaggggat aaaactcaag ggtagccctc actcaatgga tagaaccccg
     3181 cctccggtca acgccggagg cggggtctgt tatcctgcaa taggagtttt gggtgggata
     3241 gaaaaacctg tcaaaatcgc tatacttaat cca
//
LOCUS       DAOQNJ010000280         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20472_length_3264_cov_9.198816, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000280 DAOQNJ010000000
VERSION     DAOQNJ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20472_length_3264_cov_9.198816"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLG52_08640"
     CDS             complement(<1..646)
                     /locus_tag="PLG52_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNB41554.1"
                     /translation="MDPKILSQHFQTHFNAKPEIIVRAPGRVNLIGEHTDYNDGFVLP
                     MAIDREVMIALRPREDKTVRLFSLDLETESAFELSSLTREGDWIEYPKGIAYELLKAG
                     FNLKGFDAIMTGDVPRGAGLSSSAAVELAVARAFSVVSGFEWNAPAMAKLAQKAENEW
                     VGVNCGIMDQMASAACKENYALFLDCRSLEIQHAPLPKGVSVVILDTSTRRGLVD"
     gene            673..2229
                     /locus_tag="PLG52_08645"
     CDS             673..2229
                     /locus_tag="PLG52_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41555.1"
                     /translation="MDDLDLLKKYEPVLRFAKSERFFPMAVEPYLEKCALFPSGPSGA
                     AELFGHLNEPLIGKIGNLESHEFFLRFVNKPLNDFDAWIWWGVGSVLALVAGWFTLGM
                     AGVESVIGLSLSTALVLFMLASPIRLRIIPAMLAVLVFLVLGIAPIWLFFKPIPGVSI
                     AIEYLVLLPIYLLFLFYLLVRTLKFMIEKILPEGPGMVMDMLSQSTEKIAKEAARQYA
                     EIIREHRQPTYYGRVVRETDADGHSCVFLQYHFFYAFNDWRLAANGFNHHEGDWEMVA
                     VYLKDDEPHSVLLSQHGAGNIEAWETTLKALDKDGNETTHPVIYTALGSHANYSKPDV
                     IRSPAMYNPGSVQRFLFWFDGWIHYLFLLFNPNQKARQIALEEMRAKAHNLFEEHVFD
                     DLRDEEDHYVIRLPMEIATGDGLRVGFQGKNSREPMLKSSSYLKRVMSDRHVSLPPIR
                     EWRCILLNSEQGWVQYKGLWGVKSILSEESGPPGPRWDKPKRKKTGVSERVRWGKPLK
                     WLALLEKNKH"
     gene            2251..3153
                     /locus_tag="PLG52_08650"
     CDS             2251..3153
                     /locus_tag="PLG52_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41556.1"
                     /translation="MSISQALEVAIGLIFVYYVLGSIVSLITQWINESFETRGKSVER
                     HLMKIVGDKHVDEFVKLPQLQALRPIRYKNMFSFLTSSTEPKKLEKIPAATLVDSYFD
                     FVGLTSQEFKADELKDFINAFPESEGKQAMLKWVSQGVTNIEDLRKRTTTYFSGVMDQ
                     AASTFKSNARSFVIMLSIFLTLLLGTDSIQLAQTLWANASVRAIAVAQAQAVVQQEGS
                     NANVDDLLQQLLDLQIVKIGWWQTDLPQTMDIGTWGGFIALKFLGLGLTAVAVSQGSS
                     FWYDLLKKIVSPSKGGGGGDEAKG"
BASE COUNT          824 a          679 c          847 g          914 t
ORIGIN      
        1 aatctaccaa cccgcgccgg gtagatgtat caaggataac gacagagact cctttgggta
       61 ggggagcatg ctgaatttcc aaagaacggc aatctaagaa gagggcatag ttttctttac
      121 aagcagcgct tgccatttga tccataatgc cgcagttgac gccaacccat tcgttctcgg
      181 ctttttgggc gagctttgcc atggcaggcg cattccactc aaagccagag acgacagaaa
      241 aggcacgggc aaccgccaac tcaactgcag cagatgaaga gagaccggca ccacgcggca
      301 catcgccggt catgatggcg tcgaaacctt tgaggttgaa accagctttc agcaattcat
      361 aggcaatacc tttggggtat tcgatccagt cgccttctcg ggtgagagaa gataactcga
      421 aggctgattc agtttcaagg tccagagaaa agaggcggac ggtcttatcc tcacgcgggc
      481 ggagagcgat catcacttca cggtcaattg ccatgggtag gacgaagccg tcgttgtagt
      541 cggtatgttc gccaatcaag ttgacccgcc caggtgcgcg gacgatgatc tccggtttgg
      601 cgttaaaatg ggtttggaaa tgttgagaga gaattttcgg gtccatatct gcgtttatac
      661 catagaggct tcatggatga ccttgattta ctaaaaaagt atgagcctgt tttgcgcttc
      721 gcgaagagtg agcgtttctt tcccatggct gtagagcctt atttggagaa gtgtgcgctc
      781 ttcccaagcg gacctagcgg ggcggcagag ttattcggtc atttgaacga acccttgatt
      841 gggaaaattg gtaatttgga aagccatgaa ttttttctgc gcttcgtaaa caaaccgttg
      901 aacgactttg atgcatggat ctggtgggga gttggatctg tgcttgcgct ggtggcgggt
      961 tggtttacat tgggcatggc aggtgtggag agcgtgattg gcttatcgct ttcaacggca
     1021 ttggtgttgt tcatgttggc atcgccgata cgtttgcgca tcatccctgc catgttggcg
     1081 gttttggttt ttcttgtgtt gggcattgca cccatttggt tgtttttcaa acctattcca
     1141 ggtgtgagta ttgctattga gtatttagtt ttgttgccta tttatctttt gttcttgttc
     1201 tacttgttag tacggacttt gaagtttatg atcgaaaaga ttctgcctga aggtccgggc
     1261 atggtgatgg atatgctttc acaatctaca gagaagattg cgaaagaagc cgccaggcaa
     1321 tatgcagaga taataagaga acatcgtcag ccaacgtatt atgggcgtgt tgtgcgagaa
     1381 acagatgcag atgggcattc ttgtgtattc ttgcaatatc atttttttta tgctttcaat
     1441 gattggcgtt tggcagccaa tggctttaat catcacgaag gggattggga gatggttgct
     1501 gtctatctta aagacgatga accccattct gttttattgt cgcaacatgg ggctggaaat
     1561 attgaagcat gggaaaccac tcttaaagca ttagacaaag atggaaatga gacgactcac
     1621 cctgttattt acaccgcatt gggatcacac gctaattaca gcaaaccaga tgtgatccgt
     1681 tcgccggcaa tgtataaccc ggggagcgtg caacgcttcc tcttttggtt cgacggctgg
     1741 atccactatc ttttcctttt atttaacccg aaccaaaaag cacgtcagat tgcattggag
     1801 gagatgcgtg ctaaagctca taacttgttt gaagagcatg tcttcgacga tctgcgggat
     1861 gaagaagacc attatgtcat ccgtttgccg atggagattg ccacaggcga tggattgcga
     1921 gttggctttc agggcaagaa ctcaagagag ccgatgctca agtcttctag ctatttgaag
     1981 cgagtgatgt ctgaccggca tgtgtcgctc ccgccgattc gagaatggcg atgtatcctc
     2041 ttgaattccg aacaaggatg ggtacaatat aaaggattgt ggggtgtaaa aagtattcta
     2101 agcgaagagt cgggtccgcc cggaccacga tgggataaac ccaaacgcaa gaagaccggt
     2161 gtaagtgagc gtgttcgttg gggcaagccg ctaaaatggc ttgcgctact ggaaaagaat
     2221 aaacattaat gcaactcaaa ggagaaagat atgtcaattt cacaagcgct tgaagtagcc
     2281 attggcttga tctttgttta ttatgtgtta ggttcgatcg tttctctcat cacacaatgg
     2341 atcaatgaat cgttcgaaac acgtggtaaa tcagtggaac gacacctaat gaagattgtc
     2401 ggcgataaac atgtggatga atttgtaaaa cttccgcagt tgcaggcatt acgccctatt
     2461 cgttataaga atatgtttag cttccttaca tcttctacag aacctaaaaa actagagaag
     2521 atccctgctg cgactttggt cgattcttat ttcgattttg ttgggctcac ttctcaagaa
     2581 tttaaagccg atgagttaaa agactttatc aatgctttcc ctgaatcaga aggcaaacag
     2641 gcgatgttga agtgggtaag ccagggtgtg accaatattg aagacctgcg aaagcgtacc
     2701 actacctact tctctggtgt gatggatcag gcggcttcaa cctttaagtc aaacgctcgc
     2761 agttttgtca ttatgttgtc tattttcttg accttgttgc ttggcacaga tagcattcag
     2821 cttgcacaaa ccttgtgggc aaatgcgagt gtccgtgcta ttgcggtggc acaggcacaa
     2881 gctgtggtgc aacaagaagg cagcaatgcc aatgtggatg acctccttca acaattgttg
     2941 gatctacaaa tcgtaaagat tggctggtgg caaacagacc ttcctcaaac catggacatt
     3001 ggcacgtggg gcggatttat tgctttgaag tttctcgggc ttggtttgac cgctgtggct
     3061 gtttcgcaag gctcgtcctt ctggtatgat ttactcaaga agattgtctc tccatccaaa
     3121 ggtggtgggg gtggggacga agcgaaagga taataaaaaa gaggcacctg ttcaggtgcc
     3181 tcttttttat tgtttacacc acactctcag cgacggtctt tccgtacccg agcagggctt
     3241 tgaccatttc ttgtgtgcgc cctt
//
LOCUS       DAOQNJ010000281         3260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20524_length_3260_cov_8.370047, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000281 DAOQNJ010000000
VERSION     DAOQNJ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20524_length_3260_cov_8.370047"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLG52_08655"
     CDS             complement(<1..290)
                     /locus_tag="PLG52_08655"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNB41557.1"
                     /translation="MTEKKKVVVAMSGGVDSSVAAALLKEQGYDVTGMMMRLWSEPGK
                     EESNRCCTPDSMAQARRVAGILDIPFYVVDAKDVFRETVVQYFLDGYANGETP"
     gene            384..1973
                     /locus_tag="PLG52_08660"
     CDS             384..1973
                     /locus_tag="PLG52_08660"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNB41558.1"
                     /translation="MSTKADIVIENAKVFTSNKEAPFAEAVAVKGNRIIYVGTNEGAK
                     ALKDSNTRVVDGQGRTLTPGFIDSHFHLLWGAISMGGAQLYDAKDINDVKTVLLEHAA
                     KNKTAEWVEGRGVRYDVIANRHELDAIIADKPIYINAYDGHTSWANTKALELAGILQP
                     GKEAEGVGLIVRDENGLATGELRETAMGLVSDLIPEPSEARKRELLKMTMANMNATGV
                     TSVHNMNGDMQELMTFAAVEDAGEMTVRVYEPYHIKPETTEEMLSEAAEMAKVKGEFV
                     RGGAAKFFMDGVWESYTALNVDPYADVPDAKPDGIYSLEHFTRMATLCDKMGMQIFVH
                     CCGDGAVRRTLDGYEAVQKANGKRDSRHRIEHIEVYHPDDLPRFKQLGVLPSMQTSHA
                     PFSIAEGDVWPTRVGKQRWHLSFAWRDILNAGNQIAFGSDWPVAPYSPIINLHVGLNR
                     EPWAEGVPSHNLTLEELIIGYTRDAAYAEFMENEKGQIKEGYLADLVLFSHDLFALPK
                     ENIMETKPVLTMVNGKVVYEA"
     gene            1982..2956
                     /locus_tag="PLG52_08665"
     CDS             1982..2956
                     /locus_tag="PLG52_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41559.1"
                     /translation="MGTYALRRILQTIPILFIISILLFLMVRAAPGGPLTAARRNPNI
                     SKEQIEAIEEKLGLNDPLPVQYGRWLGDMLRGDMGDSIKFRRPVSEMIGERVPNTLLL
                     VGASFLVTLLFAIPIGILSARKPYSKFDYAMTTVTFIGQAIPVYWLGLGLIVIFYVTI
                     KNPFTGNPLFPVGGMNTRGMEGNLLDTLWHLVLPVTALSLGWVAWYSRFLRSSMLDVL
                     HEDYVRTAHAKGLTDSLVYYKHALQNAILPLVTLIALDLPSLFAGALFVETIFSWPGM
                     GRLFWDAAKGRDYPVLLGVVMLTAVLIILCNIIADLAYGWLNPQVKYE"
     gene            2949..>3260
                     /locus_tag="PLG52_08670"
     CDS             2949..>3260
                     /locus_tag="PLG52_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HNB41560.1"
                     /translation="MNESSPLIKEQSMAAVITRRFFKHKLAGVAITVLALLVLFSALA
                     SLTPYEPTEQNPTNIFQKASGVHWFGTDELGRDVFTRILYGGRVSLAVGLLSTLLSIS
                     LG"
BASE COUNT          793 a          827 c          839 g          801 t
ORIGIN      
        1 ggcgtttcgc cattggcgta gccgtccaga aaatactgca ccaccgtctc gcggaaaacg
       61 tccttggcat ccaccacata aaaaggaatg tccaagatcc ctgccacgcg gcgcgcctgc
      121 gccatggaat caggcgtgca gcagcggttg gactcttcct tgccaggctc actccacagg
      181 cgcatcatca tcccggtcac gtcatagccc tgctccttga gcaaagctgc cgcaaccgag
      241 gaatccacgc cgccagacat ggcgacaaca actttcttct tttcagtcat tcctgaaatt
      301 ataaactact gctgtgcgct ttctcttgcc cgtcagtgtc agataatgta tattcaggtc
      361 acaccactca aggagattaa gacatgagca ccaaagcaga tattgtcatt gaaaatgcaa
      421 aggtctttac cagcaacaaa gaagcaccgt tcgcagaagc cgttgctgta aaaggaaatc
      481 gcatcattta tgtagggaca aacgaaggtg ccaaggcgct aaaagactcg aatacccggg
      541 tcgtggatgg acaaggtcgc actctgacgc ccggcttcat cgattcacat tttcacttac
      601 tttggggtgc catctcgatg ggtggagcgc agttgtatga tgccaaagat atcaacgacg
      661 taaaaaccgt tctattggaa catgccgcca aaaataaaac tgccgaatgg gttgaaggac
      721 gcggcgtacg ctatgacgtg atcgcgaacc gccatgagtt ggatgccatc attgcagata
      781 aaccaattta tatcaatgcc tatgacggtc acacgtcttg ggcgaacacc aaagccttgg
      841 agttggctgg cattctccag ccggggaaag aggcggaagg cgtcggattg atcgtgcggg
      901 atgagaacgg gctcgctacc ggtgaattgc gcgagactgc aatggggttg gtctccgacc
      961 tgatccctga accgagcgaa gcccgcaaac gtgaattgct caagatgacg atggcaaata
     1021 tgaacgccac cggcgtgacc agcgtccaca acatgaacgg cgacatgcaa gagttgatga
     1081 ctttcgctgc ggtagaagat gcgggtgaaa tgaccgtccg cgtgtatgag ccatatcaca
     1141 tcaagccgga aaccaccgaa gagatgctca gtgaagccgc tgagatggca aaggtcaaag
     1201 gcgagttcgt gcgcggcggt gcggcaaaat tctttatgga tggggtctgg gaatcataca
     1261 ctgcactcaa cgttgaccct tacgccgatg tgccagatgc caaacctgat ggcatttatt
     1321 cgctcgaaca tttcacacgc atggcaaccc tatgcgacaa aatgggcatg cagatctttg
     1381 tccattgttg cggcgatggt gcagtgaggc gaacgctcga tgggtatgaa gccgttcaaa
     1441 aagcgaatgg gaagcgtgat agtcgtcacc gcattgaaca cattgaggtc tatcatcccg
     1501 atgacctgcc acgcttcaaa cagttgggcg tgttgccatc catgcaaacc agccatgctc
     1561 cctttagcat tgccgaagga gatgtatggc ccacacgggt aggcaagcaa cgttggcatc
     1621 tttcgtttgc atggcgcgat attttgaacg ctggcaatca gattgccttc ggcagcgact
     1681 ggcccgtagc gccctacagc ccgataatca atttgcatgt cggtttgaat cgtgaacctt
     1741 gggcagaagg tgtgccatcc cacaacctga cccttgagga actcattatt ggatataccc
     1801 gcgatgcggc gtatgccgag tttatggaaa atgaaaaagg tcaaatcaaa gaagggtatc
     1861 ttgcagattt ggtcttgttc tcgcatgacc tatttgcttt gcccaaagaa aacattatgg
     1921 aaaccaagcc ggtcttgacc atggtaaatg gcaaggtcgt atacgaagcc tagaggtact
     1981 catgggaact tacgccctgc gtcgcatttt acaaaccatt cccattcttt tcatcatcag
     2041 tattttattg tttctgatgg tgcgtgccgc gccgggcgga ccgttaacag ccgctcgcag
     2101 gaacccaaat atttcgaagg aacagattga agccattgaa gaaaagctcg gattaaatga
     2161 tcctttacct gttcaatatg gacgttggct cggcgatatg ctgcgcggtg atatgggcga
     2221 ctcgatcaag tttcgccgcc cggtttcaga gatgatcggt gaacgcgtgc ccaatactct
     2281 gttattggtg ggcgcctctt ttcttgtgac cttgttattt gccatcccca ttggcattct
     2341 ctccgctcga aaaccctact ccaaattcga ttacgccatg acgacggtta cttttattgg
     2401 gcaggcgatt ccagtttatt ggctggggct gggactcatc gtgatctttt atgtcaccat
     2461 caaaaatccg ttcacgggga acccgctttt cccggttggt ggaatgaaca cccgtggcat
     2521 ggaaggaaat ttgctggaca cgctctggca tttggtcttg cccgttaccg ccctaagttt
     2581 aggatgggtc gcttggtatt cacggtttct acgctccagc atgttagacg tgttacacga
     2641 agattacgtc cgcacggcac atgcaaaagg gttaacagat agtcttgtct attacaaaca
     2701 tgctttgcaa aatgcgattc tgccactggt caccttgatc gcgcttgact tgcccagtct
     2761 ttttgcgggt gcgctatttg tagagaccat cttctcctgg cccggtatgg ggcgcctctt
     2821 ttgggacgcg gcaaaggggc gagattaccc cgtcctcctt ggggtcgtga tgctcactgc
     2881 cgttcttatc atcctctgca acatcatcgc ggacctggct tatggctggc tcaacccaca
     2941 ggtgaaatat gaatgagtcg agtccgctta tcaaagaaca aagcatggca gctgtcatca
     3001 cgcgtcgctt cttcaagcac aaactggcgg gtgttgccat tacagtcttg gcattattgg
     3061 tgttgttctc agcgttggca agcctaacgc cctatgaacc cacagaacag aacccgacga
     3121 atatttttca aaaagcatcc ggcgttcatt ggtttggcac cgacgaactt ggtcgggatg
     3181 tgttcactcg catcttatat ggcgggcgag tctcgttggc agttggcttg ctatccacgt
     3241 tgttatccat ttcgttaggg
//
LOCUS       DAOQNJ010000282         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20635_length_3250_cov_7.509859, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000282 DAOQNJ010000000
VERSION     DAOQNJ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20635_length_3250_cov_7.509859"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..614)
                     /locus_tag="PLG52_08675"
     CDS             complement(63..614)
                     /locus_tag="PLG52_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41561.1"
                     /translation="MLNRISNFFYAVSTGWLTLTGLVVFILFMIFVLPQQAQKAEAYS
                     GGTSPDTSYIYSASDLYQMAESYGAEGRAAYIYARFTFDLVFPLAYLFFLTTSISWLL
                     ARGLSENSRWRMLNLFPLAGAIFDYLENISTSLVLARYPSQTPVVDVLAPVFTAIKWV
                     FVNGSFVVLLIGMLLFLWKRLKK"
     gene            complement(621..>3250)
                     /locus_tag="PLG52_08680"
     CDS             complement(621..>3250)
                     /locus_tag="PLG52_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNB41562.1"
                     /translation="ETHSAIVEPGVILANLNRAASKHGLMFGPDPASAERATMGGVIG
                     NNATGAHSILYGMSADHLLEAEVILSDGSSATLNSSTFNLQSSNVRLTNIVSTAHEIR
                     TKYSESIKESYPKSWRNSAGYRLNYLLPWSPSVPPQWSGENYPANLEHGAVNLAHLLA
                     GSEGTLAVIRKLKVNLVQKPKHTILGILSYTSIVEACDDVPRLLAMKPSAVELIPQLI
                     LRNARSIPAYARQMGWVIGDPAAVLVVEFSGDQPSALKETVRKLGDVLTIAESPEDQA
                     RIWNIRKVGLGILDSRPTSARPIAFIEDCAIPVERLGEFVREVEQILNAHGTFGGIYA
                     HASAGCLHIRPVLDLQHGEAVRNMRNIIEQVFALTMSLGGAMSSEHGDGIARGEFIER
                     TYGREVTEAMRLLKKAADPDNLLNPKKLFDAPPMDTNLRYGETYQAQAWKPVLTFEHE
                     RGLEGAVESCNGQGVCRKTNGVMCPSFQATRDEAFSTRGRANLLRALMRSDVDFSVPR
                     NSELELATYQSLDLCLACKGCTSECPSGVDMPKLKYEFMNQYYKSHPRHLRDYLFGYF
                     HVATKLLMPFAPMANWFMQIGWSKKIVARVLGISEHRPFPKFVRGTRQVHEMQFDKQV
                     IFVSDVFSHYIEPEVEEAAVEILNSLGYEVKILPTIGAGASLLSKGFLNAAQRHAEKV
                     LAEIKRLDSGAGLSVVGCEPPELYCLKHEYESLLPNRKEEIESIKKRTWLVDEFILRV
                     VNEAKMDISHVDSSEQNNRLTLHPHCHHRAEGPAADGLPSGVSATVDMLKQFGYEVDV
                     IDAGCCGMAGTFGYDAEHYELSMKVGELGVLPKVRDKKLENRELVASGAACRMQIKQG
                     ADVDARHPLLLVKERLISK"
BASE COUNT          929 a          849 c          657 g          815 t
ORIGIN      
        1 ggtggttttg ccgaactgaa ggtcggcgtc gtaagataaa aacttttcag gttccgcgcc
       61 atctattttt tcaagcgttt ccacaagaac aacaacatac caatgagaag aacaacaaaa
      121 cttccattca caaagaccca cttaatcgcc gtaaacaccg gtgcaagaac atcaaccaca
      181 ggcgtctgag acgggtaacg tgctaacacc agtgaagtgg aaatattttc caagtaatca
      241 aaaatagctc ccgctagcgg aaagagattt aacatccgcc agcgggagtt ctcagaaagt
      301 ccacgcgcca gcaaccagct tatagatgtt gtcagaaaaa ataagtaagc cagcggaaat
      361 accaaatcga atgtgaaacg tgcataaata taagcagccc gcccttctgc gccataactc
      421 tctgccatct gatacaggtc acttgcagag taaatatatg aagtgtctgg tgaagttcca
      481 ccgctatacg cctccgcttt ttgagcctgt tgaggcaata cgaaaatcat aaaaaggata
      541 aagacaacca accccgtcag ggtcagccag cctgtagaaa cagcataaaa gaaatttgaa
      601 attctgttca acatagcacc tcacttggat ataagtcgtt cttttaccaa taacaatgga
      661 tgacgtgcat ccacatctgc accttgctta atctgcatac gacaagccgc accgcttgcc
      721 accaattctc tgttttctaa tttcttgtct ctcactttcg gcaacacacc caactcccct
      781 actttcattg atagctcgta atgttcagca tcatagccaa aggtgcctgc catgccacaa
      841 caacctgcat caattacgtc cacttcatag ccaaattgct tgagcatatc tactgtagct
      901 gaaacgccag aaggtaaacc atcagcggca ggaccttctg cgcggtggtg acagtgcggg
      961 tggagagtga ggcggttatt ttgttcagaa gagtccacat gtgaaatatc cattttagcc
     1021 tcattgacta cacgcaaaat aaattcatcc actaaccacg ttctcttctt tatggattca
     1081 atttcctctt tgcgatttgg caataacgat tcatattcat gtttgagaca atacaactcg
     1141 ggcggctcgc atccaactac gctcaaacct gctccactat ccaagcgttt aatttctgcc
     1201 aacacttttt ctgcatgacg ctgtgcagca ttgagaaatc ccttcgaaag caaagaagcg
     1261 cctgctccaa tagttggcaa aattttcact tcatacccca acgagttcaa aatctcaaca
     1321 gcagcctctt caacctcagg ttcaatataa tgtgagaaaa catccgaaac aaaaattact
     1381 tgtttatcaa attgcatttc atgcacttgg cgtgtacccc gaacaaattt tgggaaaggt
     1441 ctgtgttctg aaatacccaa cacacgtgcc acgatcttct tcgaccagcc tatttgcata
     1501 aaccaatttg ccataggggc aaatggcatc aacaattttg tagcgacatg aaaatagccg
     1561 aacagataat ctcgcaagtg gcgtggatgc gatttgtaat attgattcat aaactcatat
     1621 ttgagtttcg gcatatccac accacttggg cattcggatg tgcagccttt acaagcaagg
     1681 caaagatcga gtgactgata ggttgctaat tcaagttcag aatttcgagg cactgaaaaa
     1741 tcaacgtccg agcgcatcaa tgcccgcagt aaattagctc gccctctcgt actaaaggct
     1801 tcatcgcgcg ttgcctgaaa cgatgggcac atcacaccgt tcgttttacg acatacgcct
     1861 tgcccattgc atgactcaac tgccccttcc aaacctcgct cgtgttcaaa ggtcaataca
     1921 ggcttccaag cctgcgcctg atacgtttcg ccataccgta agttcgtatc catgggtggc
     1981 gcatcgaaca attttttagg gttcaaaaga ttatcggggt ctgcggcttt cttcagcaag
     2041 cgcatggctt cggtcacttc acgcccgtaa gttcgttcaa tgaattcccc acgcgcaatt
     2101 ccatcgccat gttcgctgct catggcaccg cccaaagaca tggtcaacgc aaaaacttgc
     2161 tcaataatgt ttcgcatgtt ccggacagct tcaccatgtt gcaaatctaa cacaggtcga
     2221 atatgcaaac agcctgccga agcatgtgca taaattccgc caaaagtccc atgagcatta
     2281 agaatctgtt caacttcacg aacaaactcc cccagccttt caacgggaat agcacaatct
     2341 tcaataaatg caatcggtct ggcagacgtt ggacgtgagt ctaaaatccc caaccccact
     2401 ttgcggatgt tccaaatgcg agcctgatct tcaggcgact ctgcaatagt caacacatcg
     2461 ccaagcttac gcaccgtttc tttcaaagcc gacggttggt cgccactaaa ttccactacc
     2521 aacacggctg ccgggtctcc aatcacccaa cccatctgcc gcgcatacgc cggaatacta
     2581 cgtgcattcc gcagaatcaa ctgcggaatc aactccactg cgctaggctt cattgccagc
     2641 aaacgcggaa catcatcaca cgcttctacg atactggtat acgacaaaat tccaagaatc
     2701 gtgtgcttag gcttttgaac aaggttaact tttaacttac ggataaccgc caaagtccct
     2761 tcgctgcctg ctaatagatg tgccaagttc actgcgccat gttccaagtt agccgggtag
     2821 ttttctccac tccattgcgg cggcacggat gggctccatg gcaaaagata attaaggcga
     2881 taacccgccg agttgcgcca agattttggg taactttcct tgatggactc agaatacttt
     2941 gtacgaatct cgtgagctgt agaaacaata tttgtcaatc gaacatttga agactggaga
     3001 ttgaatgttg agctattaag tgtggcggaa ctcccatcac taagaatcac ttctgcttcc
     3061 agtaaatggt ctgcgctcat accataaagt atggaatgtg cgcctgtagc gttgttacca
     3121 atgacgccgc ccatcgttgc gcgctctgca gatgcagggt cgggtccaaa catgagtcca
     3181 tgcttggagg cggcgcggtt aagatttgca aggatcacac caggctcaac aatagcagaa
     3241 tgagtttcgg
//
LOCUS       DAOQNJ010000283         3248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20663_length_3248_cov_6.815221, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000283 DAOQNJ010000000
VERSION     DAOQNJ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20663_length_3248_cov_6.815221"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2562
                     /locus_tag="PLG52_08685"
     CDS             <1..2562
                     /locus_tag="PLG52_08685"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41563.1"
                     /translation="DIPEWVDKLVLQLLAKRPEERHASAKEVLRDIEERSTAPTFASL
                     ISIAPRPKNNLPTQLSTFIGREKEVAQIKGRMEKNRLVTLTGSGGIGKTRLSIQVASE
                     LLAEYPIGVWLVELAPLTDPALVPQSVCAALGVKPEGDTSALDALINYMHEKKILLVV
                     DNCEHLIDACAQLCDSLLHACPELRIIASSREALGIEGENAYRVPSLSLPNPKSGLQV
                     IEESEAVMLFMERATTILPKFEMTESNAHAIAQICQRLDGIALAIELAASRVKMLKVE
                     QIATRLDDAFRLLTGGSRTALPRHQTLRALIDWSYNLLPEEERTFLRRLSVFMGGWTL
                     EAAETVCGNADALDLLTHLVDKSLVSVDLEHSDKPRYYLLETVRQYAREKMVESEDSS
                     LLRDSHLEYFVKTAEHIAPELYTRKMPYWLDYLETEYANLQASLEWTQERNIDAGLRL
                     CNALFRLWQNRGEFTKEGLEWYKKFLSMNPNKQNATRAWAFAHAAELASLQSPTWISP
                     EIQGWFEESLNLARELGDEVCIATVLEQLGFIEYMKTGNFDKSRNLLEQSLAMSRRIK
                     YDRGVGFALDWLGTITAEQSDFSSARPLMEESLLILSRIGELRRWGLVLNRTGRMYEA
                     HGGWNEAQNYYEQALSVANEAQSRIHISLNIESLGSLAIGMGDFDRAISLLEQARKLV
                     QGTPDDKFLPFALLDLGEVARFQGNFEQAIVFFHERLSLPQGDDQKAMAYCYLAHTES
                     LRGEILKARQYFVTGLHTIPTGDDYVSPSLIPFVAYFMVGQKMDKKATSLLGWVDGWD
                     KAKGYYQLPVYQAEFDRYLAQARKGLSESEFNEAWAEGQSMSQEQVLALAMEVLQ"
     gene            2959..>3248
                     /locus_tag="PLG52_08690"
     CDS             2959..>3248
                     /locus_tag="PLG52_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41564.1"
                     /translation="MLRKLFALTALLALLTGCAPASSSVQPDPVSTQDSSSSTVPINS
                     STSTGFTLTSTDATDGGQLPIEFTCDGDGSTLPLTWSGAPDGTQSYAVIMHHV"
BASE COUNT          830 a          767 c          805 g          846 t
ORIGIN      
        1 gatattcctg aatgggtaga caagctggtt cttcaattgc ttgccaagcg tcccgaagaa
       61 agacatgcct ctgcgaaaga ggttctgcgg gatattgaag aaagatccac tgcgcctaca
      121 ttcgcatctt taatttccat tgcaccccgg ccaaagaata acctacccac ccagttgagc
      181 accttcatcg gacgcgaaaa ggaagtggcg cagatcaagg gacgcatgga aaagaatcgt
      241 ctggtgacgc tgaccggctc aggcggcatc ggcaagacgc ggctttccat tcaagtggcg
      301 tcggagttat tggctgaata tccaatcggc gtctggctgg tggaactcgc cccgctgacc
      361 gaccccgcgc ttgtgccgca atctgtttgt gctgccttgg gagtcaagcc ggaaggcgac
      421 acctctgcac tggatgccct gatcaattac atgcatgaga agaaaatatt gttggtggtg
      481 gataactgcg aacatctgat tgacgcttgc gcgcagcttt gtgattccct tttacatgcc
      541 tgccctgagt tgcggatcat cgccagcagc cgcgaagcct tggggatcga aggcgagaat
      601 gcctatcgtg tgccgtcgct ctcgctgcct aatcccaaga gtggattgca ggtcatcgaa
      661 gaatcagaag cggttatgtt gttcatggaa cgtgctacaa cgattttgcc taaatttgaa
      721 atgactgaat caaacgctca cgctattgct caaatttgcc aacgcttgga cgggatcgca
      781 ttagccatcg aacttgccgc atcgcgcgtg aagatgttga aagtggagca gatcgcaacg
      841 aggttggatg atgcctttcg attgctgaca ggcggcagcc gcaccgcctt gccgcgccat
      901 cagactttgc gtgccttaat tgactggagt tacaacttgc ttcccgaaga agagcggact
      961 ttcttgcgaa ggctctcggt cttcatgggc ggctggactc tcgaagcagc ggaaacggtt
     1021 tgtggaaatg cagatgcgct tgatctgtta actcatctgg tagataaatc ccttgtttct
     1081 gtggatctcg aacatagtga caagcctcgc tactatctgc ttgaaaccgt gcgtcagtat
     1141 gcgcgcgaga agatggttga gagtgaagat agttctcttt tgcgggatag tcatcttgaa
     1201 tactttgtga aaactgctga gcacatcgcg cctgagttat atactcgcaa aatgccctat
     1261 tggcttgatt acttggaaac cgaatacgcc aatttacaag cctcattgga atggacgcag
     1321 gagcgtaata ttgatgcggg cttgcgtctc tgcaatgcat tgtttcgatt gtggcaaaac
     1381 cgtggagaat ttactaaaga agggttggaa tggtataaaa aattcctgtc catgaatccc
     1441 aataagcaaa atgcaacccg cgcatgggcg tttgctcatg cggcggaatt agcgagcctt
     1501 caatcaccaa cttggatttc cccggaaata caggggtggt ttgaagaaag tttgaatttg
     1561 gcccgtgagt tgggagatga ggtttgcatc gcgaccgtct tggaacaact tggttttatc
     1621 gaatacatga agacggggaa ttttgataaa agccgcaact tgcttgagca aagcttggct
     1681 atgtcacgca ggataaaata cgaccgcggc gttggctttg ctctcgattg gctgggaaca
     1741 ataactgcgg aacaatcaga cttttcttca gcccgccctc ttatggaaga aagcttattg
     1801 attcttagcc gcataggcga acttagaaga tggggtttag ttcttaatcg gactgggaga
     1861 atgtacgagg ctcacggcgg ctggaatgaa gcccaaaatt attatgagca agccctgtca
     1921 gttgctaatg aagcgcagtc acgtatacac atatctttaa acattgaatc ccttggaagc
     1981 ctggctatcg gaatgggaga ttttgatcga gcaatttcct tgctggagca agcccgtaaa
     2041 cttgtgcagg gcacgcctga tgacaagttt ctaccatttg cgcttttaga ccttggtgag
     2101 gttgctcggt ttcaaggaaa tttcgagcag gcaattgtct tttttcatga gagattatcg
     2161 cttccgcaag gggatgacca aaaagccatg gcgtattgtt atttagcgca cactgaaagc
     2221 ctgcgtggcg aaatattaaa agcgaggcaa tactttgtaa ctggcttgca cactatccca
     2281 actggggatg attatgtttc cccatcctta atcccctttg ttgcttattt tatggtgggt
     2341 cagaaaatgg ataaaaaagc aacatctttg cttggatggg ttgatggttg ggacaaagca
     2401 aagggatatt atcaacttcc agtgtatcaa gctgaatttg atcgttatct ggctcaggca
     2461 cgcaaagggc tttctgaatc cgagttcaat gaggcttggg cggagggaca gtccatgagt
     2521 caggagcaag tccttgcact ggcaatggag gttttgcaat aacggtgcgt actatgaggt
     2581 gagatatttt tctccaccca caaacggaca cgaatcattg ccgacctggc aaaaagagcc
     2641 ctcgtccctg catacccaag ccctataatc caaaagagtc tctattaact cccctctatc
     2701 ttttcggtga cgtagaggat gatgtttgat gaggctgatt tgtaactaga taaatttttg
     2761 acaaaatggc atccaaagat tcttttccat ctcgattaaa atctaaacac tatcttccct
     2821 gtgcggcata tattgtatac gggtataagt ttcgacctgc agttactgca taaacagccc
     2881 aacgtaaatc atcttcaaac accatctgaa caacttctta ataaggaatt gctattgtat
     2941 gggggaaagg aaaacgacat gcttcgcaaa ctcttcgctc ttactgctct actcgctctc
     3001 ctgacgggat gcgcccccgc ctcatcctca gttcagcctg atcctgtttc gacacaggac
     3061 tcttcatcct caactgttcc catcaattct tccacatcca cagggtttac cctcaccagc
     3121 acagacgcca ctgacggcgg acaactgccc atcgaattca cctgtgatgg tgacggctcg
     3181 actctccccc ttacctggag cggcgcacca gacgggactc aaagttacgc cgtcatcatg
     3241 caccacgt
//
LOCUS       DAOQNJ010000284         3240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20746_length_3240_cov_6.872527, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000284 DAOQNJ010000000
VERSION     DAOQNJ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20746_length_3240_cov_6.872527"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PLG52_08695"
     CDS             complement(<1..976)
                     /locus_tag="PLG52_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41565.1"
                     /translation="MSNQPLVIENLTFKYKTRPELALENVSLELKQGELLLIAGSSGC
                     GKTTLARCINGLIPRSYRGDRSGKVLLHGKEVADLQIPEMAQVVGTLLQDPERQIVAS
                     NVFNEVAFGPENLGLPRAEIVSRVDQALKRLNLEYLRERETFNLSGGEKQKVALAGVL
                     TMNPSILLLDEPLASLDPASAHEALAVFRSLADEGKTVILVEHRVEDAIGIKPDRLLY
                     MEEGKIKYLGPITDLPKEIDHTQVKLPAPWVVQRVKALDIEVKTPTQPKVEERGEPLV
                     VFEDVDFQYDEELPFILKGVNLKIYPGDLIAVLGPNGAGKSTLVKHAIG"
     gene            complement(1021..2055)
                     /gene="thrC"
                     /locus_tag="PLG52_08700"
     CDS             complement(1021..2055)
                     /gene="thrC"
                     /locus_tag="PLG52_08700"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNB41566.1"
                     /translation="MTYQGLIHRYKQYLDLPAHTQIVSLNEGNTPLIPMPRLGSEFGC
                     ELFIKFEGLNPTGSFKDRGMTAAISEAVGRGAKTVICASTGNTAASAAAYAARAGLRS
                     IVLIPEGKVAVGKLAGAIAYGAEVIQIQGSFDDALSLVVEITQKTPIALVNSINPYRI
                     EGQKTSPFEICDELGSAPDWLCLPVGNAGNITSYWAGFKQYGKGLPRVLGVQAEGAAP
                     LVLGHPVDKPETIATAIRIGRPARGEQALEAAHESNGKIMAVSDAQILDAQRILASEG
                     VWVEPASAAGVAGLIAETATGKLDAKGKRVVVVCTGHGMKDPSIITESFSAPQVIPAK
                     YEALVELVGS"
     gene            2154..3038
                     /gene="thrB"
                     /locus_tag="PLG52_08705"
     CDS             2154..3038
                     /gene="thrB"
                     /locus_tag="PLG52_08705"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNB41567.1"
                     /translation="MIKIRVPATSANLGPGFDCLGLALDVWNEIAFEPAEQLKYRASG
                     EGAEKLNKGNRNLLTKAYARLHEFCGKKASGVRIAARNDIPMSSGLGSSAAAIVAGLF
                     GANEMLGKPLHKDELVKLATEMEGHPDNVAPALLGGLVASVMKHEEIITRRYEVPALT
                     VVIVKPDVEWLTKTARAVLPKSISRADAIFNIGRATLVVDALRTGDLDLLQKVMDDRI
                     HQPYRLRHISGGMAAYKCAKAFGAAALSGAGPSIILFTQPNQAERAKREIQAVFEERG
                     IDTKGIITSPGKKGVHCI"
BASE COUNT          833 a          844 c          762 g          801 t
ORIGIN      
        1 acccaatggc atgcttcacc aaagtagatt tgcccgcccc attcggtcca agcacagcga
       61 tcagatcgcc gggataaatc tttaaattga cacctttcaa gatgaacgga agttcctcat
      121 cgtattggaa atccacatct tcaaagacga ccaaaggctc gcctcgctct tcgaccttag
      181 gctgagtcgg cgtcttgact tcgatatcca acgccttgac tcgttgcacc acccacggcg
      241 ccggcagttt gacctgtgta tggtctattt cttttggcaa gtctgtgatc ggacccaggt
      301 acttgatttt gccttcttcc atataaagca agcggtctgg tttaatacca attgcatctt
      361 ctacacgatg ttccaccaag atcacagttt tgccttcatc tgccaaactg cggaaaactg
      421 ctaaagcttc atgagcagat gccgggtcga gtgaggcgag aggttcatcc agcaaaagaa
      481 tggatggatt catcgtcaac acgccggcaa gtgccacctt ctgtttctca ccacccgata
      541 agttaaaggt ctcgcgctca cgcaaatatt caagattgag gcgcttcaaa gcttggtcta
      601 cacggctcac aatctcagca cggggcagac ctaaattttc gggtccgaag gcaacctcat
      661 tgaatacgtt actagcaacg atctgacgtt cagggtcctg caacagagtc cccaccacct
      721 gcgccatttc aggaatctgc aagtcggcaa cttctttacc atgaagcaaa accttgccac
      781 tacggtcgcc acgataacta cgcggaatca aaccgttgat acagcgcgcc agcgtggtct
      841 tgccacagcc gctagagcct gcaattaata acaactcgcc ctgcttcagc tcaagagaaa
      901 cattctccaa ggctaattca ggacgggttt tatatttgaa cgtgaggttt tcgatgacga
      961 gaggttgatt agacattact tctatatttt actaggaaac ctagctttca taaagatttt
     1021 ttaactgcca accagttcaa ccaaagcttc gtacttcgca gggatgactt gaggcgcact
     1081 gaacgattcc gtgataatgg atgggtcttt cataccatga cccgtgcaca ccaccacaac
     1141 acgcttgccc ttcgcatcca acttgccggt ggctgtttct gcaatcaagc ctgccactcc
     1201 cgccgctgac gctggttcca cccacacccc ttccgaagcc agaattcgtt gcgcatccaa
     1261 aatctgcgca tcagatactg ccataatctt tccattggat tcgtgtgcgg cttctagcgc
     1321 ttgttctccg cgcgcgggtc tgcctatacg aatagcagta gcgatcgttt cgggcttatc
     1381 cacggggtga cctaacacga gcggagcggc tccctcagct tgaacgccaa gcacgcgagg
     1441 caaacctttt ccatattgct tgaaacccgc ccagtaagat gtgatattcc ccgcattgcc
     1501 gacgggcaaa cataaccaat ctggagcaga acccaattca tcacaaattt caaaaggtga
     1561 ggtcttttgt ccttcaatgc gatatggatt gatcgagttt actagagcaa tcggtgtttt
     1621 ttgggtgatc tccacaacca aagatagggc gtcatcaaaa gagccctgaa tttgaatcac
     1681 ttccgcaccg taggcaatag cgcctgccaa cttccctact gccaccttac cttcgggaat
     1741 cagcacaatg gagcgcaagc cagcccgagc cgcataggca gccgcagacg cggcagtatt
     1801 gccagtggaa gcgcagataa cggtctttgc gccgcgacca accgcctcac tgatggcagc
     1861 tgtcataccg cggtctttga aagagccggt cggattcaaa ccttcaaatt taataaacaa
     1921 ctcacaacca aattcacttc ccaagcgcgg catgggaata agaggagtat tcccctcgtt
     1981 tagcgaaaca atctgggtgt gggcgggtaa gtcaaggtat tgtttgtagc gatggattaa
     2041 gccctggtag gtcatatcaa ctccgagtta tagattgagg cgattatact catcccggtt
     2101 acaaagtgca catttttcca catttgcaac gcaggtatta tataatgtcc cgcatgataa
     2161 agatccgcgt cccagctaca tctgcgaacc ttggtcctgg ttttgattgc ctcggtttgg
     2221 cattggatgt ttggaatgaa attgcatttg agcccgctga acaattgaaa tatcgcgcaa
     2281 gcggtgaagg ggctgaaaag ttaaacaaag ggaatcgcaa cctgctcaca aaggcgtatg
     2341 cacgtttgca tgaattttgt ggcaaaaaag caagcggagt tcgcattgcc gctcgaaatg
     2401 acattccgat gagcagcggg ctcggctcaa gcgccgccgc gattgtggca ggactctttg
     2461 gcgcaaatga aatgctcggc aaaccgcttc ataaggatga attggtaaag cttgcgacag
     2521 agatggaggg acatccagat aatgtagcgc ccgctttgct cggcgggttg gtcgcttcgg
     2581 tgatgaagca tgaagaaatc atcactcgca gatacgaagt tccggcatta accgtggtta
     2641 tcgtcaaacc agacgtagaa tggttgacta aaacggctcg ggctgttttg ccaaagtcta
     2701 tttcgcgcgc agatgctatt tttaatatcg gtcgagcaac actggttgtg gatgcgcttc
     2761 gtactggtga ccttgacctc ctccaaaaag tgatggatga ccgcatccat cagccctatc
     2821 gtttgcgtca tatttctggc ggaatggcag catacaaatg cgccaaggca ttcggcgcgg
     2881 ctgccttatc tggagcaggt ccttctatta ttctttttac gcaaccgaat caggcagaac
     2941 gcgccaagag agagatccaa gctgtattcg aagaacgtgg aatagacaca aaagggatca
     3001 ttaccagccc tggcaaaaaa ggtgttcact gcatataaaa agcggacttt tccaagtccg
     3061 ctttttgttt tattttttac tcttcttgct agcaggtttt gccttcttgg cagccggttt
     3121 ctttggagaa gaaaccgcag cagaggctga aaccgcgaca ggctctgcta catagggtgt
     3181 atgcaacgcc actggctcag acttgccacg aacacggagg ttgatcattt cgaccatcac
//
LOCUS       DAOQNJ010000285         3239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20757_length_3239_cov_10.148555, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000285 DAOQNJ010000000
VERSION     DAOQNJ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20757_length_3239_cov_10.148555"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..1233
                     /gene="speB"
                     /locus_tag="PLG52_08710"
     CDS             337..1233
                     /gene="speB"
                     /locus_tag="PLG52_08710"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNB41568.1"
                     /translation="MTEEGTHGYWGDLHRPDLTPEQTDFSIIGIPFDGLASARKGAAL
                     APERLRYWSRHLTPFSEDRTRLKDMTICDLGDIEIKNPETDFVTVRQRVAALPNMPII
                     LGGDHSVTIPVLQAQRERYKDQRLGILWIDAHPDLCDFFDGSRLSHANTMRRALDFGI
                     EAHDVCMVGLRSWEEQEIDLIENGGIHVYTAADVAERGIRHIADSIYNILNDCDAVHV
                     SLDIDCLDPSVAPGTGIPEFGGLTSRDVLTLIKSMQDLSVVGLDVVEIAPSLDPSEAT
                     VFAGLKIIMEYIAVMARKKQKG"
     gene            1237..1530
                     /locus_tag="PLG52_08715"
     CDS             1237..1530
                     /locus_tag="PLG52_08715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB41569.1"
                     /translation="MDSEKLEVIHNPTESRFEIRLDGQLAKLDYVEDGDSIVMTHVGV
                     PMEFRGHGIAAIITKAGLDYAKVKSLRVIPMCSYVAAYLRRNPQYIELTRSKE"
     gene            1530..1994
                     /locus_tag="PLG52_08720"
     CDS             1530..1994
                     /locus_tag="PLG52_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB41570.1"
                     /translation="MDAPLLFRRATIQDLPAIVHMLADDDLGSQRERDEDPLPETYLR
                     AFHEIDTDKNHELIVAELSGNIVGTLHLTFLPSISYQGGLRSQVESVRVDSKLRSQGI
                     GAKMMQYAIIRAKERGAYLMQLTSHQMRINAHRFYEKLGFKKAHVGMKLILK"
     gene            complement(2024..2527)
                     /locus_tag="PLG52_08725"
     CDS             complement(2024..2527)
                     /locus_tag="PLG52_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNB41571.1"
                     /translation="MKTIVVGLGNPILGDDGVGWRVAEDIQKQLPQFLSQAEGKIDVT
                     RLSLGGLGLMEQLIGYDRAILIDAFETDDQPGSILIMKLNDLPNYTAFHTPSARDVSL
                     QKAIELGRMLGAKLPKEVEVVGITTSKVQDFSEELSPPIADVVSFAARIVLDLLQQIQ
                     IHKEQYA"
     gene            complement(2563..2814)
                     /locus_tag="PLG52_08730"
     CDS             complement(2563..2814)
                     /locus_tag="PLG52_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41572.1"
                     /translation="MKTKPIEQTEAVEDRNAMLEKALIEEYLKEKGYTLEAVKRLPAE
                     LAEKLWKEASQYASLKMEEVEARAHFIKELHDDTSPLTE"
     gene            3017..>3239
                     /locus_tag="PLG52_08735"
     CDS             3017..>3239
                     /locus_tag="PLG52_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB41573.1"
                     /translation="MNLFDRFEQNAHNHPEKTAFIYFDRGLWKTLTYKDLLHKTQRFI
                     RGLEAGHYTPGSTAALLTPPSADFFPFALA"
BASE COUNT          874 a          796 c          697 g          872 t
ORIGIN      
        1 agcggaagga ctttttcgat atcctcaaca gaattgaggc ggttggaaag ctgccagcgc
       61 cagtcgttcc acttttcgtc gggaacatcg gcgtaaatct tagcgcgttt ggaaacaaac
      121 tcggttttca tggttcctcc attagttgta aatagatcag aatatcaaca ttaaaaaaag
      181 ccactcacat tggagggtcg tggtcgggga ggtaaaaacc tgctatgccc ataaaggtga
      241 cgttcttcgg cataattgca ttttacgggc aaacgatagg ggggtcaatg cgggtagttt
      301 tcaaaggtaa aatagccaaa tccaaggagc ataacgatga ccgaagaagg tacccacggc
      361 tactggggcg acctacaccg cccagacctc acccccgaac agaccgattt cagcattatt
      421 ggaattccct tcgatggact tgccagcgct aggaaaggag cggctctcgc tcccgaacgc
      481 ctgcgttact ggtccaggca tttgactccc tttagcgaag accgcactcg cctgaaagat
      541 atgacgatct gcgatttggg tgatatcgaa atcaaaaacc ccgaaaccga ttttgtcacc
      601 gtgcgtcaac gtgttgcggc attgccaaac atgcccatca ttctcggcgg cgaccattct
      661 gtgacaatcc ctgttttaca agcccaacgc gaacgataca aagaccagcg cctcggcatt
      721 ttatggatcg atgctcaccc agacctatgc gatttcttcg atgggtcacg gctttctcat
      781 gccaacacca tgcgccgtgc cttagacttt ggcattgaag cccatgatgt atgtatggtg
      841 gggttgcgct cttgggaaga acaagaaata gacttaatcg aaaatggcgg gattcatgtg
      901 tatacagctg ctgatgttgc cgagcgtggc attcgccata ttgcggatag tatttacaac
      961 atcttaaatg actgtgatgc cgtccatgtt tcactggata ttgactgcct cgacccatcc
     1021 gttgcgccgg gcacgggcat tcctgaattc ggcgggctta cttcacgcga tgttctaacg
     1081 ttgattaagt cgatgcaaga tttatcggtt gtaggcttgg atgtagtgga gatcgctccg
     1141 tcacttgacc cttctgaagc aactgtattt gccgggttga aaattatcat ggaatacatc
     1201 gctgtcatgg cacgtaaaaa acagaaaggc taatacatgg actctgaaaa attggaagtc
     1261 attcataacc caacagaaag ccgctttgag attaggcttg atggtcaact cgccaaattg
     1321 gattatgtag aggatggcga ttcaattgtc atgacccatg ttggtgtacc catggagttt
     1381 cgtgggcatg gcattgccgc catcattacg aaggcaggtt tggattatgc caaggtaaag
     1441 tccttacgcg tcattcccat gtgctcgtat gtggcagcct atcttcgccg caacccacaa
     1501 tacatcgaat tgacccgttc aaaagaataa tggacgcccc gcttctcttt cgccgtgcca
     1561 ctatacaaga cttgcctgct atcgtccaca tgttggcgga tgatgattta ggtagtcagc
     1621 gtgaaagaga tgaagaccct cttcctgaaa cctatctccg agcatttcat gaaattgaca
     1681 cagataaaaa ccacgaactg attgttgcag aattaagtgg aaatattgta gggacacttc
     1741 acctaacctt tctgccctcc attagttacc aaggcggatt aaggtctcaa gtagaatcag
     1801 tgcgtgtgga ttcaaaactc cgtagtcaag gcattggcgc aaaaatgatg caatatgcca
     1861 ttatccgcgc caaggagcga ggtgcatatc taatgcaact tacttcgcac caaatgcgca
     1921 taaatgctca tcgtttttat gaaaaactag gctttaaaaa agctcacgta ggcatgaagt
     1981 tgatcttaaa ataagaactc cggaattttc cggagttctt attttaagca tactgttcct
     2041 tgtggatttg aatttgctgt aacaggtcta gcacgatgcg tgcagcaaag gaaaccacat
     2101 cggcaatggg cggagataat tcttcactaa aatcttgcac cttagaggtg gtgattccaa
     2161 ccacttcgac ttctttaggc agttttgccc ccaacattct ccctaactca attgcctttt
     2221 gaagcgaaac atcgcgcgca ctgggtgtat gaaaagcagt gtaatttggc aaatcattta
     2281 acttcatgat caagattgag ccgggctggt catcagtttc aaaagcatcg atcaagattg
     2341 cacggtcata tccgatgagc tgctccatca gccctaatcc acccaaagaa aggcgagtca
     2401 catcaatctt gccttctgct tgtgacagaa attgtggaag ctgtttttga atatcttcag
     2461 caactctcca tccaacccca tcatcaccga gaatagggtt acccagccca actacgatcg
     2521 ttttcatgat gcctccaccg cccaactcaa caaatatgta tattattctg taagaggaga
     2581 agtatcgtca tgtaattctt tgatgaagtg tgcgcgagct tccacctctt ccattttcaa
     2641 cgaagcatat tgtgatgctt ctttccaaag cttttcggca agttccgcag gcaagcgctt
     2701 aactgcctcc aatgtatagc ctttttcttt caagtattct tcaatgaggg ctttttcaag
     2761 catcgcgttg cgatcttcaa cggcttccgt ctgttcgatc ggtttggttt tcatttcatg
     2821 ctccgatttt atttggaacc ctgaaccctc atgcacctat cgttcaaaac agatgcattt
     2881 tcctgctcgg cataaaccat tcgcagtctt gaattcctcc caatatcatt attgcaacac
     2941 cactcttata agcacagtac taaagataat cgtacaacac tgacaaacat ccacacaaag
     3001 ggatacaatt ttgaccatga atctctttga tcgcttcgaa caaaatgccc ataatcaccc
     3061 cgaaaaaaca gcctttattt attttgatag gggactctgg aaaacgctca cctacaaaga
     3121 cttattgcac aaaacccaac gcttcattcg tggacttgaa gctgggcatt acaccccagg
     3181 cagtaccgcc gccttattaa ctccccccag cgcggacttc tttccctttg ccctcgctt
//
LOCUS       DAOQNJ010000286         3223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20927_length_3223_cov_10.652778, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000286 DAOQNJ010000000
VERSION     DAOQNJ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20927_length_3223_cov_10.652778"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLG52_08740"
     CDS             <1..528
                     /locus_tag="PLG52_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41574.1"
                     /translation="IVHLFASALFESQNDIVQSTIQGIWIESPVNYRTLVPEIHSGAL
                     SYFNSDEPGFFSKYYNEFSLLFTLGPVLVSLYFTLIASFQAKQKSTASSYNLELAKLL
                     ADLTYSKNVRTVQYIERSLFESLNEVVEAMEEGKISTEDLNIYSLVWEKAMDAVRRRY
                     DLLKKKPKRKKPVRK"
     gene            525..1553
                     /gene="tsaD"
                     /locus_tag="PLG52_08745"
     CDS             525..1553
                     /gene="tsaD"
                     /locus_tag="PLG52_08745"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNB41575.1"
                     /translation="MNELKSARILAIETSCDETACAILENGRALLSSVVASQMEIHAR
                     YGGVFPEVASRQHVLSIMPVVQQALAQARLALSDIDAIAVTQGPGLAGSLIVGMNMAK
                     GMALALDKPLVGVNHLEGHIYSSWVYEAGDHKPPAPQFPLMALLVSGGHTELNLMTDH
                     LTYQRLGATLDDAAGEAFDKVARLLDLPYPGGPSIQREAEEGDPNRFNFPRAWLDGTW
                     NFSFSGVKTSVLYEVNDLKKRSNNIPVHDLAASFQKAVVDVLFKKTMDAARKYGAKEI
                     LIAGGVSANRALRQAFKAQTEFPVHIPPLALCTDNAAMIGAAGYFRYALGHASAMDID
                     VLPAWPLS"
     gene            complement(1747..2919)
                     /locus_tag="PLG52_08750"
     CDS             complement(1747..2919)
                     /locus_tag="PLG52_08750"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNB41576.1"
                     /translation="MKALFENLWSRISDLAYIETDNETIQRKGFLLSLYHLLILGILS
                     YTTINTLLYLLFYPSLEYGIYLLEDVVMFGLFYVFWRLTKRGNISLIGHISITLTILL
                     AIVFSDPKYLEYSMVIFALPIAISSFTLRPAMSFAYAFLISFVYILYSFIQSYVWEYN
                     LTPIVALFALAVTTWVISQQLENAISKNEKLVKDQQKTYDELKDAYETTLEGWSKALE
                     IRDQETQGHSKRVSDLTLRIARRMGFNDEQLVHVSRGVLLHDIGKIGIPDEILHKPGP
                     LNEKETRIMRLHPQISVDLLSPIEYLKPALSIPKYHHEKWDGSGYPHGLSGEMIPLEA
                     RIFAIVDVYDALSHDRPYRKAWSKEKAIEHIKSETGKHFDPAVTLIFLEEIEKKKR"
     gene            complement(2954..>3223)
                     /locus_tag="PLG52_08755"
     CDS             complement(2954..>3223)
                     /locus_tag="PLG52_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41577.1"
                     /translation="TQTMRRLPIYGREPTLALDGGEDGLDIYRRLLNVAPNWLAPHGM
                     ILLEIEATQGIKALNLATDIFSESSISLHQDFAGHDRLLEIIPRL"
BASE COUNT          872 a          679 c          757 g          915 t
ORIGIN      
        1 attgtgcatt tatttgcctc tgctttgttt gaaagccaga atgatattgt tcaaagcact
       61 attcaaggga tttggatcga gtctcctgtg aattatcgga cgctcgtgcc ggaaatccat
      121 tctggtgcgc tgtcatactt caatagcgat gagcccggtt ttttctcaaa gtattacaac
      181 gaatttagct tgttgtttac actcggacca gtcttggtct ctttatattt cacattaatt
      241 gcgagctttc aggcaaaaca gaaatccacg gcatctagtt acaacttgga gttggcgaaa
      301 ttactggctg acctgacata ctcaaaaaat gttcgcacag tgcagtatat tgagcgtagt
      361 ctcttcgagt cgcttaatga agttgtggaa gccatggaag aaggaaagat ttccactgaa
      421 gatttgaata tctattcgtt ggtttgggaa aaagccatgg atgctgtgcg ccgacgatat
      481 gatttgctga aaaagaagcc aaaacgaaag aaacctgtac gcaaatgaat gaattgaaat
      541 ctgctcgtat tcttgccatc gaaacttctt gtgatgaaac tgcctgtgcc attctcgaaa
      601 atggacgggc tttactttct tctgtagttg cctcccaaat ggagatccat gctcgttatg
      661 gcggcgtgtt ccctgaagtt gcatctcgcc agcatgtgtt gagtatcatg ccggtcgtgc
      721 agcaagcgtt ggcacaagct cggctagccc tcagtgatat tgatgccatc gcagtcacgc
      781 aagggcccgg tcttgccggt tcactgatcg tgggcatgaa catggcgaaa ggcatggcac
      841 ttgcgttgga taagccgttg gtgggtgtca accatcttga aggacatatt tattcttcat
      901 gggtgtatga ggctggagat cacaaaccgc cagcaccgca attcccattg atggcattgc
      961 tcgtttccgg cgggcacacc gagttaaatc ttatgaccga ccatctgacc tatcagcggc
     1021 ttggtgcgac tctcgatgac gcggcagggg aggcgttcga taaagtggcg cgcttgctgg
     1081 atttgccata ccccggtgga ccgtctattc agcgtgaagc agaagaaggc gacccgaacc
     1141 gcttcaactt ccctcgcgcc tggctcgatg gaacctggaa tttctccttc agtggagtga
     1201 aaacctcggt attgtatgaa gtgaatgatt tgaaaaaacg cagtaataac atccctgtcc
     1261 atgatttggc tgcttcattt caaaaagccg ttgtggatgt gttgttcaaa aagaccatgg
     1321 atgctgctcg taaatatggc gcgaaagaaa tattgatcgc tggtggcgtc tctgccaatc
     1381 gtgccttgcg ccaagccttt aaggcacaaa cagaatttcc tgttcacatt ccacctcttg
     1441 cactttgtac agacaacgct gccatgatcg gggcggcggg atacttccgc tatgcattgg
     1501 ggcacgcttc tgccatggat atagacgttt tgcccgcttg gccgctttcg taattgaacg
     1561 taggctctaa aatgaattgc cccattaaag gaccaaaccc ccgttcgttt gcccaaacga
     1621 ttgggggttt gtcatcagga gaatgcaact attattatat ttgggcagtt taatggttcc
     1681 tgaaccttga ttaaggattg gattagagga ttaagggagg aaagattaaa ttttgtgaag
     1741 atatttttat ctcttctttt tttcgatttc ttctaaaaag ataagagtca cagccgggtc
     1801 gaaatgtttc cctgtttctg attttatatg ctcaatcgcc ttttcttttg accaagcttt
     1861 tcggtaggga cggtcatggg aaagcgcatc gtacacatcc acaatagcaa agatgcgagc
     1921 ttctaaggga atcatttctc ctgatagccc atggggatat ccgctaccgt cccacttttc
     1981 atggtgatat ttgggaatgg acaaagcagg tttgagatat tcaatagggg agagcaaatc
     2041 cacagatatt tgcggatgca acctcatgat gcgagtctct ttctcgttta gcggacctgg
     2101 cttatgcaaa atctcatcgg gaattccgat ctttccgata tcgtgtaaca aaacccctct
     2161 agatacatgt acaagctgtt cgtcattgaa tcccatacgg cgagcaatgc gcagcgtgag
     2221 gtctgaaacc cgtttggaat gaccttgggt ctcctggtct cgaatttcaa gcgctttcga
     2281 ccagccttct aatgttgttt cataagcatc ttttagctca tcatatgtct tttgctgatc
     2341 ttttactagt ttctcatttt tgctgattgc attttcaagt tgctgtgaaa tgacccaggt
     2401 tgttaccgca agagcaaaca aagcaacaat aggtgtgagg ttgtactccc aaacatagct
     2461 ttggataaaa gaatacagga tgtaaacaaa actaattaag aaagcgtatg caaacgacat
     2521 ggcaggtcgt aacgtaaagc tggagattgc gatcggcaat gcaaagataa ccattgagta
     2581 ttcgagatat tttgggtcac taaacacaat agcaagcaaa atggtcaacg tgatcgaaat
     2641 atgaccgatg agagaaatgt ttcctctttt ggttagacgc caaaaaacat aaaacaaccc
     2701 gaacataacc acatcttcaa gtagatagat tccatattcg aggcttggat agaaaagaag
     2761 ataaagcaaa gtgttgatgg tggtgtaact caaaatcccc aaaatcagta ggtgatataa
     2821 ggaaagcaaa aagccctttc tttgtatggt ctcgttatct gtctcaatat atgccaagtc
     2881 tgaaatacgc gaccataggt tttcaaagag tgctttcatt aagatatgcc tagatttgat
     2941 tatatctttt gttttagagg cgtggaataa tttcgagcaa acggtcatga cctgcgaagt
     3001 cttggtgcag tgaaattgaa gattcagaaa aaatgtctgt tgcgaggttc aaggctttaa
     3061 tgccttgcgt tgcttcaatt tcaagcagaa tcatgccatg aggggcaagc caattcggtg
     3121 caacgttcaa aagacggcga taaatatcta aaccatcttc gccaccatcg agagccagtg
     3181 tgggttctcg cccatagatg ggaagcctgc gcatggtttg ggt
//
LOCUS       DAOQNJ010000287         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_20982_length_3218_cov_6.454316, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000287 DAOQNJ010000000
VERSION     DAOQNJ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20982_length_3218_cov_6.454316"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLG52_08760"
     CDS             <1..189
                     /locus_tag="PLG52_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41578.1"
                     /translation="QYSTCPVSFHQISIQIFSPNAEIPAAFPFLPVCFGRMMTTSGFF
                     CSAPARATGIYKDIQTTT"
     gene            467..>3218
                     /locus_tag="PLG52_08765"
     CDS             467..>3218
                     /locus_tag="PLG52_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41579.1"
                     /translation="MPITPQEFISKWKRVTAREKQTYQEHFLDLCQLVGHQTPNDYDP
                     TGTRFAFEMGAAKTSGGQGWADVAKLGFFGWEYKGKDADLDKAYEQLLRYRDALQNPP
                     VLVVSDINFIVIRTNYTNLPTRTYTLALDDLLKPESIQLLKKVFSSPEDLKPKETIES
                     VTREAAEKFAKLARNLREYGETPQDVAHFLIRLLFCLFAEDIGLLPERLFPRLLEQTR
                     RNAGDFQEVLRQLFRAMNTGGYFGSDKILHFNGGLFDDDRVLKMDGADMDIIADIDGL
                     DWGAIKPSIFGTLFERGLDPSKRSQLGAHYTGEDDILLIVEPVLMKPLREEWEKVKEE
                     CRKWKVESESTTDKKKKDLQKKIQGALLGFMDKVAAVKVLDPACGSGNFLYVALRLLL
                     DLQNEVAAFAEEMGMPRPFVTVTPAQLYGIETNEYAHELAQMTIQIGYIQWLRDNGYG
                     QPKEPILKKSQNIRHRDALMSYGPNALMDRDELLETIDTALKSAPEQGKIVIEPEWPA
                     VDVIIGNPPFLGGSKLRSELGNEYVEALFKLYGDRLPVSDLVCYWFEKARAMIENGKA
                     KRAGLLATQSIRGGANRTVLERIKKTGDIFWAQSDREWILDGATVHVSMIAFDNGLEK
                     EKLLDNQVAPEIHADLSGTTNLTNIFDLVENANICFRSDEKGGPFDIDEQTAKRMLSV
                     QNNPNGKPNSDVVRPYFNAKDVLQNWQKSWIIDFGADTTIEEASGYEAPFEYIKQNVK
                     PIRDNVRNPREKKYWWLHRRPAPDMRKAIQNLERFIVTPAVSKHRIFAWLSGNAIPDH
                     QLHVIARQDDYFFGVLHSKFHELWARATGTQLREAESGFRYTPTTTFETFPFPYAPGS
                     EPKNQEVAAIASAAKELVEQRDRWLNAEGLSDADKKKRTLTNLYNGRPTWLELAHKKL
                     DDA"
BASE COUNT          917 a          741 c          799 g          761 t
ORIGIN      
        1 cagtatagca cttgcccggt gagttttcat caaatttcca tacagatttt ttcccctaac
       61 gcagaaatcc cagcggcctt cccattttta ccggtttgtt ttggacgcat gatgacgacc
      121 tccggctttt tctgctcagc ccctgccaga gccacaggga tttataaaga tatacaaacg
      181 accacttaaa attagctggt gttaaaaata aatatacccc atctgcaggg tgatgaatat
      241 tacagacact tgccatttct atcataatga aaatggacac tgcctgcatt tgtcttttat
      301 accgtttttg ggggataaaa gtcctgccct taactgcatc ccattgcata ccgactgtca
      361 aaagcaaaca gggcggcaga aaccgcgcaa taccatctgg aatcaggaag caagcttcct
      421 gattccagat acacaatggg tataataccc aaaagggaaa caacccatgc ccatcacccc
      481 gcaggaattt atcagcaagt ggaagcgtgt gaccgcacgc gaaaagcaga cctatcagga
      541 acattttctt gatctgtgtc aactcgtggg gcatcagacc ccgaacgatt atgacccgac
      601 cggcacgcgt tttgcttttg agatgggcgc ggcgaaaacc agcggcggac aaggctgggc
      661 agatgtggca aagctcggtt tttttggttg ggagtataag ggcaaggatg ccgacctgga
      721 taaagcgtat gaacagcttc tccgttatcg cgatgcgctt cagaatccgc ccgtgcttgt
      781 ggtctccgac attaacttta tcgtcatccg aaccaattac accaacctgc ccacgcgcac
      841 gtatacgctg gcgctcgatg acctgctcaa gcctgagagc attcaactgc tcaaaaaagt
      901 ttttagcagc cccgaagatc tgaaacccaa agagacgatt gaaagcgtaa cgcgtgaggc
      961 ggcagagaag tttgccaagc tcgccaggaa cctgcgcgag tacggtgaga cgccgcaaga
     1021 cgtggcgcac tttctgattc gcttgttgtt ctgtttgttc gccgaagata ttggtctgtt
     1081 gcctgaaagg ctcttcccgc gcctgctcga acagaccagg cgcaacgcgg gcgattttca
     1141 agaagtattg cggcaactct tccgcgccat gaacacgggc ggatactttg gctcggataa
     1201 gattctgcac ttcaatggcg gcttgttcga cgatgaccgc gtgctgaaaa tggacggcgc
     1261 ggatatggac atcattgccg atattgacgg cttggattgg ggcgcgatca aaccgtctat
     1321 ctttggcacc ttgtttgagc gcggacttga cccttccaaa cgttcacaac tcggcgcaca
     1381 ttacaccggt gaagatgata ttttgttgat cgtggaaccg gtgttgatga agccgttgcg
     1441 ggaggagtgg gagaaggtta aggaagagtg tagaaagtgg aaggtcgagt cggagagcac
     1501 gacggataag aaaaagaaag acttgcaaaa gaagattcaa ggcgcgttgc ttggctttat
     1561 ggacaaggtc gccgctgtaa aagtgcttga ccctgcctgt ggcagcggga acttcttgta
     1621 tgtagccttg cgcctgttgt tagatttgca aaacgaagtg gctgcctttg ccgaagagat
     1681 gggcatgccg cgcccctttg tgaccgtgac cccggcgcag ttgtatggca ttgaaaccaa
     1741 cgagtacgcg cacgaactgg cgcagatgac gattcagatc ggctacattc aatggctgag
     1801 agataacggc tatggtcagc ccaaagaacc gattttgaag aagtctcaga acatcaggca
     1861 cagagatgca ctgatgtcgt acggtcccaa cgcgttgatg gatcgagatg aattgctaga
     1921 aacgatagat acggcactga agagtgcgcc cgagcaaggt aagatcgtga ttgaacccga
     1981 gtggcccgcc gtggacgtca tcatcggcaa cccgccgttt ttgggtggaa gcaaattacg
     2041 aagtgaactt gggaatgaat atgttgaagc tctattcaaa ctttatggcg acagattgcc
     2101 ggttagcgat ttagtttgtt attggtttga aaaagcccgc gccatgattg aaaacggtaa
     2161 agcaaaacgg gctggtttgc ttgctactca atcaattcgc ggtggtgcta acagaacagt
     2221 tttagaaaga ataaagaaaa ctggtgatat tttttgggca caatctgacc gtgaatggat
     2281 actggatgga gcaacagtcc atgtttcaat gattgctttt gataatggat tagagaaaga
     2341 aaaactctta gacaatcaag ttgccccaga aattcatgca gatttatcag gcacaacaaa
     2401 tttaacaaat atttttgact tagttgagaa cgcaaatatt tgtttcagaa gcgatgaaaa
     2461 aggcggtcca tttgatattg atgaacaaac cgctaaacga atgctgagtg ttcaaaacaa
     2521 cccaaatgga aagccgaatt ccgatgtagt acgaccatat ttcaatgcaa aagatgtatt
     2581 gcagaattgg cagaaaagct ggattattga ctttggagcc gatacaacta tagaagaagc
     2641 atccggctat gaagcacctt ttgaatatat caaacaaaac gtaaaaccaa ttcgtgataa
     2701 tgtaagaaat ccaagagaga aaaagtattg gtggctacac agaagaccag ccccagatat
     2761 gagaaaggct attcaaaatc tagaaagatt cattgtcacg ccagcagttt caaagcaccg
     2821 aatctttgct tggctttcag gtaatgcaat tcccgaccac caacttcatg ttattgcccg
     2881 tcaagatgat tatttctttg gggtcttaca ctctaaattc cacgaacttt gggcaagagc
     2941 aacaggtact caactgcgag aagctgaaag tggttttcgt tatacaccca ctaccacctt
     3001 tgaaaccttc cccttcccct acgcgccggg ctcggagcca aagaaccaag aggtcgccgc
     3061 catcgcgtcc gctgcgaaag agttggtcga acaacgggac agatggctca atgccgaagg
     3121 tctcagcgac gccgataaga aaaagcgcac actcaccaac ctgtataacg gacgtcccac
     3181 ctggctggaa ctggcacata agaaactcga tgatgccg
//
LOCUS       DAOQNJ010000288         3212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21032_length_3212_cov_9.151093, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000288 DAOQNJ010000000
VERSION     DAOQNJ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21032_length_3212_cov_9.151093"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..790
                     /gene="pyrH"
                     /locus_tag="PLG52_08770"
     CDS             74..790
                     /gene="pyrH"
                     /locus_tag="PLG52_08770"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNB41580.1"
                     /translation="MAELKYKRILLKLSGEALGGSSGFGIDVNEAESIASRIKEVYDM
                     GVDVAVVIGAGNLWRGKQGLERGMDRSTADYMGMLATVMNAMALMDALERQGVFTRVL
                     SAIEMRAIAEPYIRRRAVRHLEKRRVVIFGAGTGNPFFSTDTAAALRATEIDAQVVIK
                     ATKVDGVYDSDPKKNPDAKKFDQLSYIDVLNRRLEVMDSTAITLCMENKIPILVVNLW
                     DSKALTSALYGEKVGTLVSG"
     gene            965..1534
                     /gene="frr"
                     /locus_tag="PLG52_08775"
     CDS             965..1534
                     /gene="frr"
                     /locus_tag="PLG52_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNB41581.1"
                     /translation="MSTDEVKSLLKDAETRMHGAIQSLVDDLNGIRTGRASPALVERL
                     PVEYYGTPTPLQNLASISVPEPRTLTIKPFDAGSLKDIEKAIRSSDLGLNPNSDGKVI
                     HLNLPPLTEERRRDLVKHMSHRLEESRVAVRNVRRDLHNDIREFEKEKLITEDDLKRG
                     EDDLQKLTDKFIEEIARLGQNKEKEIMEV"
     gene            1578..2324
                     /locus_tag="PLG52_08780"
     CDS             1578..2324
                     /locus_tag="PLG52_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNB41582.1"
                     /translation="MTDTPLNIPLEKVPQHVAMIMDGNGRWAIQRGLPRLAGHKAGTE
                     NLRRVIRATVEFGVKYLTIYAFSTENWGRPAEEVNGLMLILQNVIDRELNELHNEGVQ
                     LRHIGRLERLDPAIQKKVLHAIELTKNNDRLVLNVAFNYGGRDEIVNAIQNIIKDGIP
                     AEEVTDEMVNRYLFTAGVPDPDLIIRTSGELRVSNFLIWQAAYSEWYITPTFWPDFDK
                     EEYHRALATYANRDRRYGKVSSGELQESNA"
     gene            2317..3129
                     /locus_tag="PLG52_08785"
     CDS             2317..3129
                     /locus_tag="PLG52_08785"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNB41583.1"
                     /translation="MRRRLITALGLAVVGVPAILFGGFLYYLLMGTFLIGAAWEYVHL
                     FRAVKFDPNMIVTVGGVAAVTLTRMFLPQFAQPTLAIVVLLAMAVHLIAYERGRDQSA
                     LDFGVTIGGIVYIGWIGSYLLDLRNLPEGGWWFMLVMFCVWAGDSGAYSIGRAYGNHK
                     MAPRLSPKKSWEGYAASVFTGAHTGALYVWIFQTFGHHIYSITIWQGALMGLFLGALP
                     TLGDLGESMIKRQSGIKDSSDIIPGHGGFFDRIDSWLWGSVIGFYFLTWFIL"
BASE COUNT          809 a          769 c          789 g          845 t
ORIGIN      
        1 catcaacaaa tagtttctta aaaagccagc cttcgggttg gctttttttc taattcgatt
       61 tggaggtatg agaatggctg aattgaaata taagagaatc ctgctcaagt taagtggcga
      121 agcgcttggc ggttcatccg gatttggcat tgatgttaac gaagcagagt caatcgccag
      181 ccgcatcaaa gaagtctacg acatgggcgt agatgtagca gttgtgatcg gcgccgggaa
      241 cctttggcgc ggcaagcaag ggcttgaacg cggcatggac cgctccacag cagactatat
      301 gggcatgctt gccaccgtga tgaatgccat ggcgttgatg gatgccctcg aacgacaggg
      361 cgtgtttacc cgcgtcctct ctgccattga aatgagagcc atcgccgaac cttatattcg
      421 ccgacgcgct gttcgccacc tcgaaaaacg ccgtgtcgtc atttttggcg ctggaacggg
      481 aaatcctttc ttctcaaccg ataccgctgc ggcgttacgc gctactgaaa tcgatgcgca
      541 ggttgtcatc aaagctacca aagtggacgg ggtgtatgac tcagacccga agaaaaaccc
      601 cgatgcgaaa aagtttgacc aactcagcta catcgatgtt ctcaaccgcc gtctcgaagt
      661 gatggatagc actgccatca ctctgtgtat ggaaaacaaa attccgatcc ttgtggtcaa
      721 cttgtgggat tcaaaagcgc ttaccagcgc cctatatggc gaaaaagtgg gcactttggt
      781 ctctgggtaa ttctttttat acccaagcct ctctctttcc aacggaaggg gagaggcttt
      841 tctgttccaa tagttgatag tctctctata tttttcataa actatcaatt tttttaccat
      901 cccatagcct aaattcttgt tttaaggtat ccttgtattg taattatttt cttggaggaa
      961 gatcgtgagt actgacgaag taaagagttt gttaaaagac gccgaaaccc gcatgcacgg
     1021 cgcgatccaa tcccttgttg acgacctgaa tggcatacgt actgggcgcg ctagcccggc
     1081 attggtggaa agacttccgg tcgaatacta tggcacccca accccgcttc aaaatttagc
     1141 ttccattagt gtgcccgaac cacgcacatt gaccattaag ccttttgatg ccggctcgct
     1201 caaggatatt gaaaaagcga ttcgctcctc agaccttggg ctgaatccca actccgatgg
     1261 taaggtcatt cacttgaacc tgccgcccct tacagaagaa cgccgccgcg atttggttaa
     1321 gcacatgagt caccgccttg aagagtctcg ggtggcagtt cgcaatgtgc gccgtgatct
     1381 gcataacgat atccgcgaat ttgagaagga aaagcttatc actgaagacg acctcaaacg
     1441 cggtgaagac gaccttcaaa agcttaccga taaattcatc gaagagatcg ccagacttgg
     1501 tcaaaacaaa gaaaaagaga tcatggaagt atagccatcc cctttgatgc cttttggcat
     1561 tgaaaaggtc gatccatatg acagataccc ctttgaacat tccgcttgaa aaagtcccac
     1621 aacatgttgc catgattatg gatggcaacg gacgttgggc aattcaacgt ggacttcccc
     1681 gcctcgctgg gcataaagca ggaactgaaa atctgcgtcg cgtcatccgc gcaacggtcg
     1741 aatttggtgt gaaatacctc accatttacg cgttctccac cgaaaattgg gggcgccctg
     1801 ctgaggaagt gaatgggtta atgctcatcc ttcaaaatgt gattgatcgg gaattgaacg
     1861 aacttcataa tgaaggtgtt caacttcgtc atattggtcg tcttgaaagg ctcgaccctg
     1921 ccattcaaaa gaaagtcttg catgcgattg agttaaccaa aaataacgac cgcttggtgc
     1981 tcaatgtagc attcaactat ggcgggcgtg atgaaattgt caatgccatt caaaacatta
     2041 tcaaagatgg catccctgct gaagaagtga ccgatgaaat ggtcaatcgt tacttattta
     2101 cagcgggcgt gcccgacccg gatttgatta tccgtacatc gggtgaactg cgtgtcagca
     2161 acttccttat ctggcaggca gcatactcag agtggtacat cacaccaacg ttctggccag
     2221 actttgacaa ggaagaatat caccgtgcgt tggcaactta tgccaatcgc gacagacgat
     2281 acggaaaggt atcttcgggg gaattacagg aatccaatgc gtagacgtct cattaccgct
     2341 ttgggattag ccgtagtagg agtgcccgct attttatttg gcgggttcct ttattactta
     2401 ttaatgggga cctttttgat cggcgctgcg tgggaatatg tacatctatt cagggctgtg
     2461 aaatttgacc ccaatatgat cgtaaccgtt ggtggcgtgg cggctgtcac actcactcgc
     2521 atgttccttc cacaatttgc acagcccacc ctagccattg tcgtgttatt ggcaatggca
     2581 gttcacttga ttgcttatga gcgcggacga gaccagtccg cgcttgattt tggagtgacc
     2641 attggcggta ttgtctacat tggttggatc ggctcctatt tattagacct gcgtaattta
     2701 ccagagggtg gttggtggtt tatgttggtc atgttctgtg tatgggcagg cgactctggt
     2761 gcttactcca ttggacgtgc atatggaaac cacaaaatgg cgccgcgcct tagcccgaaa
     2821 aaaagttggg aaggctatgc agccagcgtc ttcacaggag cacacactgg tgcactctat
     2881 gtgtggatct tccaaacttt tggacatcat atttatagta ttacgatctg gcagggagcg
     2941 cttatggggc tttttctcgg cgctttgcca acgcttggtg atcttggcga aagcatgatc
     3001 aaacgccaat cgggcatcaa agactcaagc gacattatcc ccgggcatgg cggtttcttt
     3061 gaccgcatcg actcatggct atggggttcc gtcatcggat tttattttct cacctggttc
     3121 atactctaat ctctggagga tttcatggaa gttctcggtg caacacctac tcgaaacctc
     3181 gcgctcgagc ttgcacgtgt gaccgaagcc gc
//
LOCUS       DAOQNJ010000289         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21064_length_3209_cov_8.186747, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000289 DAOQNJ010000000
VERSION     DAOQNJ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21064_length_3209_cov_8.186747"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..681
                     /locus_tag="PLG52_08790"
     CDS             310..681
                     /locus_tag="PLG52_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNB41584.1"
                     /translation="MSKNRTQQMVDRLRELQASSPDIEASAIVSVDGLSIASALPQGV
                     EEDRVSAMSAAMLSLGERIAGELGRGSLEQVYIKGLKGYVVLISVGEEAVLTALVREQ
                     ARLGLIFLDMRRAAEDLAKLI"
     gene            692..1327
                     /locus_tag="PLG52_08795"
     CDS             692..1327
                     /locus_tag="PLG52_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNB41585.1"
                     /translation="MSSVQKSGLYYPNKFGLITIKSLEEVMGKNGLNAILNLGGLSHY
                     IENYPPDNLDKGFDFTELSAIGSSLEEMYGPRGGRGLALRAGRATFADALRNFGALAG
                     AADLAFVVLPLQSKLRIGLPAFAKIFTQLSDQQTTVEEKDTEWVWTIHKCPCCWGRTG
                     VDKPVCFIATGLLQESLKWVSGGNEFRVNESKCIAMGDPVCEFIVQKEPIS"
     gene            1344..2195
                     /locus_tag="PLG52_08800"
     CDS             1344..2195
                     /locus_tag="PLG52_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB41586.1"
                     /translation="MADAKSKILIVEDDPDVAEMLTAYFRTQDYEVFTVNWGEDGVRS
                     ALQVNPDLAILDIRLPDIDGYEVARRLRTDRRTAQIPIIFLTEKRDRADRLQGLELGA
                     DDYITKPFDVQELRLRVRNALKRVSQGSLTNPVTGLPEGALVDEKLSEVLGTDNSSLL
                     FISLLNMSAFREAYGFVASDDVLRAVSLMIVNTMREFSRPEDFLGHLSTTEFILVVPP
                     ANLAALSDKLNSRLEQSVEYFYPIKDREQAAKRSDRLGAKLIEVASLKTYTDVVKFKA
                     ELLSFKN"
     gene            2205..2945
                     /locus_tag="PLG52_08805"
     CDS             2205..2945
                     /locus_tag="PLG52_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNB41587.1"
                     /translation="MRITVVIPTYNEAENLPKLVSALFSLPLDLSLLVVDDNSPDGTG
                     QLAEDLAKQHPGRIDVLRRPGKMGLRSAYLNGFQRILDSNADAIVQMDADFSHDPVVL
                     TAMAKLLETNDVVLGSRYVSGGSVDEKWPVWRKWLSAFGNFYARTILGLPLHDVTTGY
                     RMWRSTTLKQMPFERIQATGYIFLVEMIYLAHCLKFQIGEAPIYFADRRFGKSKMSFK
                     IQMEAAVRIWQVLWNYRDLRKAGKSARI"
     gene            3068..>3209
                     /locus_tag="PLG52_08810"
     CDS             3068..>3209
                     /locus_tag="PLG52_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNB41588.1"
                     /translation="MIIERNDLRNIAIIAHVDHGKTTLVDGLLRQSHTFRENQHVEER
                     VMD"
BASE COUNT          818 a          750 c          770 g          871 t
ORIGIN      
        1 gcgatctcgg ccttgacagg gccgcatgtt aggccgctac accacgagac caattagtta
       61 agcgggcgat agtttaccat gagggtatat tcgtgtcaat acgaactttg gaaattcgtt
      121 atgcaatttc aaaagacgaa tcgcataaat tattattcga ttgtatattg tgataatgcc
      181 ttgccctttt gcaaactaca caacaacgaa cttgacacaa tgattgccat gttctaaaat
      241 tgttctgttg tttgtaaatt gacgtcctaa gattcttggg gcgtaattat tccccccagg
      301 agggctggct tgtctaaaaa tcgcacacag caaatggtgg accgcctgcg cgaattacag
      361 gcatcttcgc cggatatcga agcgtctgca atcgttagcg tggatggttt gagcattgca
      421 tctgccctgc cgcaaggcgt ggaagaagat cgtgtttccg ccatgtctgc agcaatgttg
      481 tcattaggtg aacgcatcgc cggtgaattg ggacgtggct cccttgagca agtgtatatc
      541 aagggtttga aaggctacgt tgttttgatt tccgtcggtg aagaagcggt tcttaccgcc
      601 ctcgtacgtg aacaggccag gcttggcctg atcttccttg atatgcgacg cgccgctgaa
      661 gatctggcga agttgattta gtggagtcat tatgagttcc gtacagaaat ctggtttgta
      721 ttaccccaat aagtttggtt tgatcacaat taaatcgctc gaagaagtga tgggtaagaa
      781 tggtttaaat gctattctta acctcggtgg cttaagtcac tacattgaaa actatccccc
      841 tgataacctc gataaaggtt ttgactttac agaactttct gctatcggtt cttcactcga
      901 agaaatgtat ggtcctcgtg gcggacgtgg tttggcactt cgtgctgggc gtgcgacttt
      961 tgccgatgcc cttcgcaatt ttggcgcttt ggctggtgcc gcagacttgg catttgttgt
     1021 ccttccgctt caatccaagc tcagaattgg tctgccagcc tttgcaaaaa tcttcaccca
     1081 gcttagcgac caacaaacca ccgttgaaga aaaagatact gaatgggtct ggaccatcca
     1141 caagtgcccc tgctgttggg ggcgcactgg cgtggataag ccagtctgtt ttattgctac
     1201 cggtctgctt caagaaagcc tcaagtgggt ttccggtgga aacgaattcc gcgtaaacga
     1261 atccaagtgt atcgcgatgg gtgacccggt ctgcgaattc atcgttcaaa aagaaccgat
     1321 ctcctagcca caaggcattg agcatggcag acgcaaagtc gaagatcctc atagtagaag
     1381 acgacccaga tgtggccgaa atgttgaccg cctattttcg gactcaggat tacgaagtat
     1441 ttaccgtaaa ttggggtgaa gatggcgtcc gctctgcatt gcaggtcaac cctgacctcg
     1501 ccatattaga catccgtttg ccagacattg atggctatga agttgctcgc cgccttcgca
     1561 ccgatcgtcg aaccgcacaa atcccaatca ttttcttaac cgaaaaaaga gaccgagcag
     1621 accgcttgca agggctggag cttggtgctg atgattacat caccaagcct tttgacgtgc
     1681 aggaattgcg cctgcgtgtg cgaaatgctt tgaaacgtgt cagtcagggt tcgttgacca
     1741 accctgttac cgggttgcca gaaggcgcgc tcgtagacga aaagttatct gaagtccttg
     1801 gaacagataa ttcatcgctt ctattcatat ccttattgaa tatgagcgca tttcgtgaag
     1861 cctacggttt cgtagcctca gatgatgtgt tgcgagccgt cagtttgatg atcgtcaaca
     1921 ctatgcgtga gtttagccgc ccagaagatt tccttgggca cctgagcacc acagaattta
     1981 ttctggttgt tccccctgcg aaccttgctg ccttgagcga taaacttaat tcccgccttg
     2041 aacagtcggt agaatatttt tacccgatca aagatcgtga gcaggcagcc aaacgatcag
     2101 accgccttgg tgctaaactg atcgaagttg cctccctcaa gacatatacg gatgttgtca
     2161 aattcaaagc ggagttgctc agctttaaga actaggaaag tacattgcgt atcacagttg
     2221 ttattcccac ctataacgaa gcggagaacc tacccaagtt ggtctccgct ttattttcgc
     2281 ttccactaga tttaagcctg cttgtcgtag acgacaatag ccccgatgga acgggtcaac
     2341 tggctgaaga ccttgcaaaa cagcaccccg ggcggattga tgttttacgc cgtccaggca
     2401 aaatggggct ccgctctgcc tacctgaacg gatttcaaag gatactcgac agcaatgccg
     2461 atgccattgt ccagatggat gcggattttt ctcatgatcc cgttgtgttg acagcgatgg
     2521 caaagttact tgagaccaac gatgttgttc ttgggtctcg gtatgtttcg ggcggctctg
     2581 tggatgagaa atggcccgtt tggcgaaaat ggctttctgc atttggtaat ttctatgcgc
     2641 ggacgatttt aggactcccc ttacatgatg taaccacggg ctatcgtatg tggcgtagca
     2701 caactctcaa gcaaatgcct tttgaacgca ttcaagcgac agggtacatt tttttagttg
     2761 aaatgatcta tttagctcat tgcctcaagt ttcagattgg tgaagcccca atttattttg
     2821 cggaccgtcg ttttggaaaa tccaaaatgt ctttcaaaat ccagatggaa gcggcagtac
     2881 gtatctggca ggttctttgg aactaccgag atttgcgcaa agctggaaaa tcggcaagaa
     2941 tctagtctcc cttcctcaaa ctgacgctct ctgataaaat actgcccgca agccgaacga
     3001 aaaattaccc cctcggcgaa atgtcgaggg atttttgtgc ctgtatataa ttgaagaaag
     3061 aataaaaatg atcattgaac gaaacgacct cagaaatatc gccattattg cacacgttga
     3121 ccacggaaaa accactctcg tagatggact cctacgtcaa tctcatacct tccgcgaaaa
     3181 tcaacatgtc gaagaacgtg tgatggatt
//
LOCUS       DAOQNJ010000290         3203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21128_length_3203_cov_9.303685, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000290 DAOQNJ010000000
VERSION     DAOQNJ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21128_length_3203_cov_9.303685"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..2432)
                     /gene="recQ"
                     /locus_tag="PLG52_08815"
     CDS             complement(267..2432)
                     /gene="recQ"
                     /locus_tag="PLG52_08815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNB41589.1"
                     /translation="MNTPSSILKNTFGYDTFRPLQQEVIENILARRDTLAVMPTGGGK
                     SLCYQIPSLLFDGLTVVVSPLISLMKDQVEQLRAFGVPSVFLNSSLSLHEYNENMDYV
                     KRGEVKLLYLAPETLLTPRILTLLSGLKVDLLTIDEAHCISEWGHDFRPEYRQIVDVR
                     KKFPNAVCMALTATATSRVRQDIRHTLKFATTNEFIASFNRENLFIEVVPKQDPAQQV
                     REIIDRYKDQSGIIYCFSRKQVDELAAYLTTKGYSVRPYHAGLDDAERRKNQELFIRD
                     DVQIIVATIAFGMGINKPNVRFVIHHDLPKSIEGYYQEIGRAGRDGLPAHCVLLYSYA
                     DVAKLNYFIDQKEGDEKRVAIEHLNAIVRYAEDERNCRRKPLLNYFGESYSAETCSNC
                     DNCNSAPTPLTDVTIPAQKFLSCVKRADEKFGAGHIVDILLGSKSEKVLRWEHDKLST
                     YGIGKELDRKQWMHLARQLLSMGYLKQEGEFHTLSLTPKALEALRKREPIFGVMQEAE
                     RVKKDGRKKAAELDYHRGLFAILRQKRKEMADEAGVPPYVIFSDRTLTEMAAYFPQSS
                     ESLLTISGVGQVKAQQYGAPFLEVIREFAEKHKLHEKPKGKQQEKSEVGQRTLLIGEL
                     YNGGETVQSLMAKYQVTSQTILDHLTRFVVAGNTLQHVTGLEAMTSASEAQKQAVFAA
                     FDEVGTAMLKPVFDKLNGTMNYDDLKVLRLIYMTEQGRQ"
     gene            2635..>3203
                     /locus_tag="PLG52_08820"
     CDS             2635..>3203
                     /locus_tag="PLG52_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41590.1"
                     /translation="MINLRKTFLRAIENEGRALGTPSIKFDNRKIISSGPDQDVPNGL
                     VYFLTDASGFYLSLPPNTVGIIVHPDGTIEAKSTGGLQEVRPGLYKILYVDMHDRSDS
                     TQPVTELSLDNESITLRVLFRYRVVDPILALGIERPVETLIEHLQADLAQYIRTNNHN
                     DLADRANPQESSKILQFFAQRHATRNRLSR"
BASE COUNT          842 a          795 c          708 g          858 t
ORIGIN      
        1 aaattgacac ttgccactgg ttgtcacctc ctttccgtta tggaaattgc ccaaaaggca
       61 taaaaaatcg gctgtcttac acagactcag ccgagggttt ccatcaaaaa acctgaaacg
      121 aaccgaatga tcgaacgatc aaaattgcgc ttcaattctc ctttgattac gagcggaacc
      181 gctctgtgcc gaacctgcga gcaggattat acacgattca cccacagatg agaagatgat
      241 taagcggctg gaaactgcgg caagagctac tgcctgcctt gttccgtcat ataaatcagg
      301 cgcaaaacct tcaaatcatc ataattcatt gtgccattca gcttgtcaaa aacaggcttc
      361 aacattgccg ttccaacttc atcaaaagca gcaaacaccg cctgtttttg ggcttctgag
      421 gctgaggtca tggcttccaa acctgtcaca tgttgcaagg tgttgccagc cactacaaag
      481 cgcgtcaaat ggtcaagaat cgtctggctg gttacttgat atttcgccat taaactctgc
      541 accgtctcac cgccgttgta cagttcgccg atcagcaggg ttctctgccc cacttcactt
      601 ttctcttgct gtttcccctt tggcttttca tgtagtttat gcttctcagc aaactcacga
      661 atgacctcta aaaatggggc accatactgc tgtgccttca cctgccccac cccagagatc
      721 gtcaataagc tttctgatga ttgcgggaaa tacgctgcca tctcggtcaa agttcggtct
      781 gagaagatca catacggcgg cacccctgct tcgtctgcca tttcttttct tttttgacga
      841 aggatggcaa acagcccgcg atgataatcc aactctgcgg cttttttcct cccatctttc
      901 ttgacacgtt ccgcctcttg catgacgccg aaaatgggtt cgcgcttccg aagtgcttcc
      961 aacgccttgg gcgtaaggct cagggtatgg aactcccctt cctgcttgag gtatcccatc
     1021 gaaaggagtt gacgagcaag atgcatccac tgctttctat ctaattcttt tccaatccca
     1081 tacgtggaca gtttgtcatg ctcccaacgg agcactttct cacttttaga accgagcaaa
     1141 atatctacaa tatgcccggc tccaaatttc tcgtctgcgc gttttacgca agagagaaac
     1201 ttctgtgcgg gaatggtcac atcggttagc ggagtcgggg cggagttaca gttgtcgcaa
     1261 ttggaacagg tttcggcgga gtatgattcg ccgaaataat tgagcaacgg tttgcggcga
     1321 caattgcgtt catcctctgc ataacgaaca atggcattta gatgctcaat ggctacacgc
     1381 ttttcgtcgc cttctttttg gtcgatgaaa tagtttaatt tggcaacatc tgcatagcta
     1441 tatagcaaaa cacaatgtgc aggcagacca tcacgcccgg cacgcccaat ttcttggtaa
     1501 tagccttcaa tacttttggg caggtcatgg tggattacaa agcgcacatt aggcttatta
     1561 atgcccatgc cgaaggcaat tgtcgctaca atgatttgaa catcatcacg aatgaataat
     1621 tcttgatttt tacgccgttc tgcatcgtcc agcccggcat gatacggacg gaccgaatat
     1681 ccctttgtgg taaggtatgc agcgagttca tccacttgtt tacgcgaaaa gcaatagata
     1741 atgccagatt gatctttata ccggtcaatg atctcgcgca cttgttgcgc agggtcttgt
     1801 ttgggtacaa cttcaatgaa aagattctcg cggttaaaac tggcgataaa ttcgttcgtg
     1861 gtggcaaact tgagcgtatg ccgaatatct tgccgcacac gagaggtggc tgtcgccgtc
     1921 aacgccatgc aaacggcatt cgggaatttc ttgcgcacat ccacgatctg tcggtattcg
     1981 gggcgaaagt catgccccca ctctgagatg cagtgcgctt cgtcgatggt cagcaagtcc
     2041 actttcagtc cagaaagaag cgtgagaatt cttggggtga gaagcgtttc cggcgcgaga
     2101 taaagcaact taacttcgcc gcgcttcaca taatccatat tctcgttgta ttcatgcagg
     2161 gaaagcgagc tattgagaaa aacggacggc acgccaaatg cccttaattg ctcaacttga
     2221 tctttcatga gcgaaataag tggagagacc acaacggtca agccatcaaa caatagagat
     2281 ggaatctgat aacagagtga tttcccccca ccggtaggca ttacagccaa tgtgtcgcgc
     2341 cgcgcaagga tgttctcgat cacttcctgc tgaagcggtc gaaacgtatc gtaaccaaag
     2401 gtatttttga gaatagatga aggggtattc acaagatcca ccctaaaaag gacaagatgt
     2461 ttatgggtgt gattttatca tcttcacagg taaaatttgt ctttacaatt aaaaacaggg
     2521 gtaattgtct ggagttgtct tgcagaaact acctttaagt tgtaaactct taataatcgt
     2581 gcaaacttcg ctgcttaata agcatactat ccatgggaca aggcaagagg gattatgata
     2641 aatttacgca aaacattctt aagagctatt gaaaatgaag gacgtgccct agggacacct
     2701 tccatcaagt ttgataatcg caagattatc tcctcgggtc cggaccaaga tgtgcccaat
     2761 ggattggtct attttctgac tgacgccagc gggttttatc tatctctccc cccaaatacc
     2821 gtaggaatta tcgtacaccc agatggcacc attgaagcca aaagcaccgg cggattgcaa
     2881 gaggtgcgcc cagggttgta taagattttg tatgtagata tgcacgaccg gtctgactcc
     2941 acgcagccag ttactgaact ttcattagac aatgaatcta ttaccttgcg cgttttattc
     3001 agatatcgtg ttgttgaccc gatcctcgct cttggcattg aacgcccagt agagaccctc
     3061 attgaacatc tccaagcgga tctggcgcag tatattcgca ccaataatca taatgatctt
     3121 gcagaccgcg ccaacccgca agagtccagt aagatattgc aattttttgc tcaacgtcat
     3181 gccactcgca atcgcctctc gcg
//
LOCUS       DAOQNJ010000291         3194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21223_length_3194_cov_5.726027, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000291 DAOQNJ010000000
VERSION     DAOQNJ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21223_length_3194_cov_5.726027"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            551..>3194
                     /gene="hsdR"
                     /locus_tag="PLG52_08825"
     CDS             551..>3194
                     /gene="hsdR"
                     /locus_tag="PLG52_08825"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:NF008521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
                     /protein_id="HNB41591.1"
                     /translation="MNQKTSLTSNFSHLQAFDEQLVRLGVLAERYYADDPNTSLIKMR
                     QLGELLAKHIATRMGTYQTEKEESQYELLRRLRDEGVLPYEIYLLFDQIRYAGNKANH
                     ANQGEVATALNIIKMGWQAGLWFQRTFLKEDFEAGVFVEPSATNEVEALLKEREELAA
                     KAEKANAKVEELLKQQQVKAARKKSQTFDKYKSAAKTASQSVFLDEAQTRQIIDGQLC
                     AAGWEADTVNLRYNNGTRPERSRNLAIAEYPTASGPADYVLFIGLMPVAVVEAKRKNV
                     DVSAALVQAKRYSRDISLTNEMQSPGGAWGEYHVPFVFSTNGRMENYQQLETMHGVWF
                     CDVRNSKNISRALSGWYTPEGLATLLKQDQVQAHKQLDSEPMEFGFPLRFYQRDAIRA
                     VENEIAQGKQNMLIAMATGTGKTKTCIALIYRLLKVKRFKRILFLVDRSALGEQAANA
                     FKDTRMDAIQAFSDIFGVMELDGQKPETETAVHIATVQGMVKRLLYAGEDQTPPAVDQ
                     YDCIVVDECHRGYLLDRELSDTEINFRSFDDYVSKYRRVIDYFDAVKIGLTATPALHT
                     TEIFGEPVYTYSYREAVIDGFLIDHEPPIQIKTRLSEAGIQWKAGEDVKLYYPRRNQL
                     ELFKAPDDIKLNVDEFNKKVITKQFNQVVCGYLARELDLYSPQKTLIFCANDLHADLV
                     VDLLKQEFAKRLDGVDDDAIVKITGSADKPLELIRRYKNERNPKIAVTVDLLTTGVDV
                     PEICNIVFLRSVNSRILFDQMLGRATRLCEEIGKETFRVYDAVGIYNALQDMTAMKPV
                     VVDPKISFSQLVDEIIRVKDEEERELVREQLMAKFQRKKRHLSDETKKDFETVAGISL
                     DAFINKLRTMPIDEVAKWFTENQGL"
BASE COUNT          890 a          632 c          904 g          768 t
ORIGIN      
        1 gcggcagcca gagcgaggta aagcccgcct ttgccagccc ggggatctcg cgggtgatgt
       61 agttccacca ttgaaattct ttgccgtctt ctttgggaca gtcccagtaa aaggtttgca
      121 gcatgacgcc catgtaaatc tccttgttga ttgggggcga ttgtactgga gaatggttaa
      181 tcggggatgg gtgataggta atgggggatg ggtgatgcgt aataagtaat acgtaacgtg
      241 taatgagagg tgagtaacga ggggatatag gatggtcgaa ggagcgtcga aggagtgtcg
      301 aaggatgatt agggatcgat gggggcttgt ttggagctct attggagcta ctgtggagct
      361 acattggggc agtaagagga tggtaagacg agagtaagag gggggatata ggatggatag
      421 aggatggata gaggatgagt ggatgtgttt ctttcgggta gccaaatggg aggggggtga
      481 ttatcgggta caataagaaa gaatagcggg gacaaaccct gcaaaaataa cctttttctt
      541 aaatgatgtt atgaaccaaa aaacttctct gacatctaat ttttcacact tacaggcgtt
      601 tgatgaacag cttgtacgtt tgggtgtgct tgcggagagg tattatgccg atgacccgaa
      661 caccagcctg attaagatgc gtcaattagg ggaattgctg gcaaagcata ttgcaaccag
      721 gatggggacc tatcagactg aaaaagaaga aagtcagtat gaattattgc gccgattgcg
      781 ggatgaaggc gtattgcctt atgaaatcta tctgttgttt gatcagatac gatatgctgg
      841 gaataaagcc aatcatgcaa atcagggaga agttgctacc gcactcaata ttataaaaat
      901 gggctggcag gcaggtttgt ggtttcagcg tacgtttttg aaagaggatt tcgaagcagg
      961 cgtgttcgtt gagccatctg ctacaaatga agtggaagcc ttgctcaaag aacgtgaaga
     1021 gctggcagca aaggcagaaa aagccaatgc caaggtggaa gaactactta agcagcaaca
     1081 agttaaagca gctcggaaaa aatctcaaac atttgataaa tacaaaagcg ccgcaaaaac
     1141 agcaagccaa tctgtatttt tagatgaagc acaaacccgc cagatcattg atgggcaatt
     1201 gtgcgcggca ggttgggaag ctgacacagt taacttgcgc tacaacaatg ggacgcgacc
     1261 cgagcggagc aggaatttag caatcgcaga atatcccact gccagtggac ctgccgatta
     1321 tgtgctgttc atcggcttga tgcctgtggc ggtggtggaa gccaagcgta agaatgtgga
     1381 cgtctcggcg gcattggtgc aggcaaagcg atacagccgt gatatttctc tgacgaatga
     1441 aatgcaatcg ccaggcgggg catggggcga atatcatgtt ccctttgttt tctcaaccaa
     1501 tgggcgcatg gaaaactatc agcagcttga aaccatgcat ggggtctggt tctgtgatgt
     1561 gcgtaactct aaaaatatca gccgtgcgct gagtggttgg tatacccccg aaggcttggc
     1621 tacactctta aagcaggatc aggtgcaggc tcataaacaa ttggatagcg agccaatgga
     1681 gtttggcttt cccttgcggt tttaccagcg agatgccatc cgtgctgtag agaacgaaat
     1741 tgcgcaaggt aaacaaaata tgctgatcgc gatggcaact gggacaggca agaccaagac
     1801 ctgtattgcc ttgatctatc gtttgctgaa ggtaaagcgc ttcaaacgga ttttgttttt
     1861 ggtagaccgc tcggcgttgg gcgaacaagc cgcaaatgca ttcaaagaca cacggatgga
     1921 tgccattcag gcattctctg atatttttgg cgtgatggaa ttggatgggc agaagcctga
     1981 aaccgaaacc gccgtacata ttgccacagt gcagggaatg gtgaagcgtc tcctttatgc
     2041 aggggaagac caaaccccac ctgcggtgga tcaatatgac tgcattgtgg tggatgaatg
     2101 ccatcgcgga tatttgcttg accgcgaact ctcggataca gaaatcaact tccgcagttt
     2161 tgatgattac gtttcaaaat atcggcgagt gatcgattat ttcgatgcgg taaaaattgg
     2221 gttgaccgcc acacctgcct tgcatactac cgaaattttc ggcgaaccag tttacaccta
     2281 cagctatcgt gaagcggtga tcgatggatt cttgattgac catgagccac cgattcagat
     2341 caagaccagg ctttcagaag caggcatcca atggaaagct ggcgaagatg tgaaattgta
     2401 ttacccacgc cgcaaccaat tggagttgtt caaagcgcct gacgatatta agttgaatgt
     2461 ggatgagttc aataaaaaag tcattaccaa gcaattcaat caggtggtgt gtggctatct
     2521 ggcacgcgag ctggatttgt attcgccaca aaagacgttg atcttctgtg ccaatgattt
     2581 acatgccgat ctggtggtgg atttgctcaa gcaggaattt gcaaagcgac tcgatggcgt
     2641 ggatgatgat gcgattgtga aaatcacagg cagtgccgat aaaccgttgg aactcatccg
     2701 ccgttacaaa aatgagcgca atcccaagat cgccgtgacg gtggatttgc tgaccacagg
     2761 cgtggatgta cccgagatct gcaacatcgt ctttctgcgg agtgtgaaca gccgtatctt
     2821 gttcgaccag atgctgggac gcgccacgcg cctgtgtgaa gagatcggca aggaaacgtt
     2881 ccgtgtgtat gatgcggtgg gaatctataa cgccttgcag gatatgaccg ccatgaagcc
     2941 tgtggtggtt gacccaaaga tcagtttttc gcaattggtt gatgagatca tccgcgtgaa
     3001 ggatgaggaa gaacgcgagt tggtgcgcga gcagttgatg gcaaaattcc agcgcaagaa
     3061 acgccatctc agcgacgaaa ccaaaaagga ttttgaaacc gtcgcgggca tttcactgga
     3121 tgcattcatc aataaactgc gcactatgcc gattgatgaa gtggcgaaat ggttcacaga
     3181 gaatcaggga ttgg
//
LOCUS       DAOQNJ010000292         3172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21480_length_3172_cov_10.198268, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000292 DAOQNJ010000000
VERSION     DAOQNJ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21480_length_3172_cov_10.198268"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PLG52_08830"
     CDS             <1..954
                     /locus_tag="PLG52_08830"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB41592.1"
                     /translation="AMILITGATGFIGRALVRQLSSIGYPLRALIRPSAKTPRLPKGV
                     PVEVAVVSLADTRGLRAAMRDVETVFHLASAENQGARGSLLITDIQGTQNLVEAAADA
                     GVDRIVYLSHLGAARASGYPAFKAKGIAEESIRAGKVPYTVIRTSLVYGPEDHFTNNL
                     ARLLRNSPGVFPVPNGGRVVVQPVWVEDLVTCMLWALQNESTVNQVYEVGGNEFFTLQ
                     QIIEIIMDVTRRRRFLVPLSPITLRAMTVFLESVVPGFPLSSLFLDYFAVNRTTAVDA
                     MPRHFGLMPARFSYRIDYLIYKRWYQRLWSAVTERLSPQRR"
     gene            956..1834
                     /locus_tag="PLG52_08835"
     CDS             956..1834
                     /locus_tag="PLG52_08835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB41593.1"
                     /translation="MSLPNIRLLETPDEMKLVEDLQRQVWPGSETDVVPSHVLITVVH
                     NGGLVMGAFEEEQLIGFVFGFPGLERTADGPRPKHCSHQMGIHPKYRDSGIGFELKRA
                     QWQMVRHQGLDHITWTFDPLLSRNAHLNISKLGAVCSTYRESEYGELRDGLNAGLPSD
                     RFQVDWWINSRRVEKRLSKRPRPTLKLAHVARSGLRPFYTPQFSDAGLMLAPEHVPPF
                     EDRLLLVEIPTNFMQVKEKDFPLARDWRFFSRELFQTAFAKNYIVTDFVFDANEGSPR
                     GMYVLTHGDSTLDEFE"
     gene            complement(1926..2690)
                     /locus_tag="PLG52_08840"
     CDS             complement(1926..2690)
                     /locus_tag="PLG52_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41594.1"
                     /translation="MNRSLNIQLLPLLKKMSLRRGAIFGGLILCALLAFEVFNFGTTS
                     FALRDVLGDLQFAGMYWSSVLAFAFCAIDFAGIARIFTPEQGRDEPAEVWYLFGAWLL
                     AAAFNAMLTWWGVSVAIRNHNALGGGLVGQETMMTVVPIFVACMVWLIRVLLIGTFSV
                     AGERLFSMADIYDRNTAYRNVPASYSTPQPRTVQPAPRQEVNPPVNNYPRPVPKPQPR
                     PNSMPAYGANRPEPTYHPVGMSAMSRNENSNPSVRR"
     gene            complement(2864..>3172)
                     /locus_tag="PLG52_08845"
     CDS             complement(2864..>3172)
                     /locus_tag="PLG52_08845"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNB41595.1"
                     /translation="SNLGWILIMIGLFLQLTQLAWLGILVFSGGALFALVTLPVEFDA
                     SARARRMLADSGIIQTDAEMRGVSNVLNAAALTYVAGLVTAVLQLLYYVSLVSGRSRD
                     "
BASE COUNT          770 a          801 c          853 g          748 t
ORIGIN      
        1 gccatgattc tcatcactgg cgccaccgga ttcattggac gcgccctcgt acgccaactg
       61 tcatctatcg gataccccct ccgtgctctt attcgtcctt ctgccaagac gccacgcctg
      121 cccaaaggcg tgcctgttga ggtggcggtg gtttcgcttg cagatacgcg cggtctgcgt
      181 gctgccatgc gcgacgtgga aacggtcttt cacttagcca gcgctgaaaa ccaaggggct
      241 cgcggaagtt tgctcatcac cgatattcaa gggacacaaa acctcgttga agccgctgcc
      301 gatgccggcg tagaccgtat tgtctatctt agtcatctgg gggcggcgcg tgcatcgggg
      361 taccctgcct tcaaagccaa agggattgcc gaagaatcca ttcgcgcggg caaagtgcca
      421 tataccgtca tccgtacttc attggtttat ggacccgaag atcacttcac caacaatcta
      481 gcgcgcttgc ttcggaattc accgggagtt ttccccgtcc ccaatggcgg acgagtggtg
      541 gtgcagcccg tatgggtaga agaccttgtc acttgtatgc tctgggcgtt gcaaaatgaa
      601 agtacggtca atcaggtata cgaagtaggt ggcaacgaat tttttaccct gcaacagatc
      661 attgaaatta tcatggatgt tacaaggcga cgccgttttc ttgtaccact ttcgccgatc
      721 accttgcgtg ccatgacggt gtttctcgaa agtgtggtgc cgggctttcc actttcttcg
      781 ctctttcttg attatttcgc cgtcaatcgc accacggcag ttgatgcgat gccgcgtcac
      841 tttggtttga tgcctgctcg tttctcttat cgtattgatt acttgatcta caaacgttgg
      901 taccaacgct tatggagcgc ggtcaccgaa cgattatccc cacagagaag ataacatgtc
      961 ccttcccaat attcgcctgc ttgaaactcc cgatgaaatg aaattggttg aagacctgca
     1021 acggcaggtt tggcccggct ctgagacaga tgtcgtccca agccatgtgt tgatcaccgt
     1081 tgtccataat ggcgggttgg tgatgggcgc gtttgaagaa gaacaactca ttggctttgt
     1141 ctttggtttt cctgggctcg aacgcacggc agatggtccg cgccccaagc attgttcaca
     1201 tcaaatgggc attcatccga aatatcgcga tagtggcatt ggctttgaac tgaaacgtgc
     1261 ccaatggcaa atggttcggc atcagggtct cgaccacatc acatggacct tcgacccgtt
     1321 gttgagccgg aacgcacacc tcaatatttc caagcttggc gcagtgtgca gcacctatcg
     1381 tgaatctgaa tatggtgagt tgcgtgatgg actaaacgcc gggctgcctt cagaccgttt
     1441 tcaagtggat tggtggatca attcgcgccg tgtagaaaag cgactcagca aacgtccacg
     1501 cccgactctc aaactggctc atgttgcccg cagcggactc cgcccctttt acacgcctca
     1561 attctccgac gcaggcttga tgcttgcacc cgagcatgtg ccaccctttg aggatcggct
     1621 cttgttggtg gagattccca ccaattttat gcaggtcaaa gaaaaagact ttcctctcgc
     1681 aagagattgg cgctttttca gccgtgaatt gtttcagact gcgttcgcta aaaattatat
     1741 tgtcacagat tttgtctttg acgccaatga aggcagtcca cgcggcatgt atgttctaac
     1801 gcatggtgat agcacgttgg atgagtttga ataaatttgt tcttaacgca tgagacgccc
     1861 tttcggacgt ctcagcggtt ggatggggaa gaggatttcg agaaacctta tctcacagac
     1921 aaggactatc tccttacaga gggattgctg ttctcgttgc ggctcatggc agacatgccc
     1981 acaggatgat aggttggttc gggacgattg gcgccataag caggcattga gttcggacgg
     2041 ggctgcggct tcggaacggg tcgcgggtaa ttgtttacgg gtggattcac ttcctggcgt
     2101 ggagcaggct gaacggtccg gggttgaggc gtggagtagg aagcaggaac gttgcggtat
     2161 gccgtgttgc ggtcataaat atccgccatt gaaaagaggc gttctccagc aacagagaag
     2221 gttccgatca acagcacgcg gatgagccaa accatgcagg caacgaagat ggggacaacg
     2281 gtcatcatgg tttcttgacc gaccaaacca ccgcctaagg cgttatggtt tcgaatggca
     2341 accgaaacac cccaccaggt gagcatggca ttgaaagcag ccgcgagcag ccacgcgcca
     2401 aagagatacc aaacttcggc gggttcatcg cgaccctgtt cgggggtaaa gatacgagcg
     2461 ataccggcga agtcgatggc gcaaaaggca aacgccaaaa cagaagacca atacatcccc
     2521 gcaaattgta gatcaccgag aacatcgcgc aaggcaaatg aagtggtgcc aaagttgaac
     2581 acttcaaaag cgaggagtgc gcacaagatc agcccgccaa agatcgcgcc gcgtcttagc
     2641 gacattttct taagcaaggg cagaagttgg atattgaggg aacggttcat gggaattctc
     2701 ctttttggaa ccaaaaacgg gtcaagttat ctgaagttaa atatagaaca cttgttctaa
     2761 tttgtcaagt taacgttgga ttgcaggaga attgcatgaa tggatatccc ccaaaaaaat
     2821 atcggggcgg cttgcaagcc gccccgattg atattgcata aacttaatcg cggctgcgtc
     2881 cgctgacgag tgagacgtaa tacagcagct gtaaaacggc ggtgaccaaa cctgccacat
     2941 aggtcaatgc ggcggcattt agaacattgc taacgccacg catttcggcg tcagtttgga
     3001 taatgccgct gtcggctaac atgcgtcggg cgcgggcaga ggcgtcgaac tccacgggca
     3061 aggtgacgag tgcaaaaagg gcaccgcctg agaagaccaa aatcccgagc catgcgagct
     3121 gtgtcaattg caggaacaag ccgatcatga tgaggatcca gccgagattg ga
//
LOCUS       DAOQNJ010000293         3165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21576_length_3165_cov_6.051768, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000293 DAOQNJ010000000
VERSION     DAOQNJ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21576_length_3165_cov_6.051768"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="PLG52_08850"
     CDS             complement(<1..1599)
                     /locus_tag="PLG52_08850"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB41596.1"
                     /translation="MFTITPFVLISFFALVISVYLTVVVWRRNNSLQAYYFALAQSTS
                     VFWLVINVLLYAATDVQWKIFFRILESFGYLTGQPLMLMFMLTFAGYGHIAQKKWVKA
                     ILIFYWISNIALTCAAPWLNGLFWKEYILHPNNLLEFVPGPAYGYRLISGTLCYTLPL
                     LVLLVIASLRGSNIQKRQARIMLLCLFIMVLTVTIGESAFTHIIKIEGYDTAPVQSAI
                     IGILFVWALYGHNFLDVVPVARNKLINNLSDGMIALDLKNRIIDINRSAVAVLGNTED
                     ALLGKNLEDVFPRFGSELKDIPEKEIKFELSTNDQRFYDVLISPLHDEWPQGLVGRLI
                     ILRDVTERKQTELRLLQLHKAVEQSPVSVVITDIQGNIEYVNPHFSQLTGYELNEVIG
                     KNPRIIQSGDTSLEAYEEMWRTIQSGKIWRGELLNKKKNGDTYWEVEMIAPVIDSDGI
                     IVNYIAIKEDVTAQKESDAKLREAYTRLEEQMNEIQGLQDELREQAIRDPLTGLHNRH
                     YLKEVLGRELSRAIRERYPISFLLL"
     gene            1666..3123
                     /locus_tag="PLG52_08855"
     CDS             1666..3123
                     /locus_tag="PLG52_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HNB41597.1"
                     /translation="MTKRIFISADHGMAIIYFLQSDVVSTLLEAGVEVVILTDDDTKE
                     KISQRFAKPGLTFEGLRLKQANDYAKKISPRLQSLLIYLRRVGGSRRINTEAMDSHVW
                     EVWGENGWKFRIGVWIPSAVMLFFLRNFSWARKLLVRIQNRFTPKPGIYTDLFDKYQP
                     DMVIASTPGWRMDRYLLRESAKRGIPNMTVIVGWDNSSSYNVSGADVQWATCWSQLQK
                     DELVYGSDWNPERVNIGGIPSYDGYFRKQWLMPRDEYFKLHNLDPKRKLISYASSFVH
                     FAPNFPNIEALAKLVSSDSLAEPSQLLIRLHPSHFQDKPKIFAEERQRVFDLEKKYPH
                     VHVVQPVALGGSLGYYGGEDMDEKSSMMAYSDVLVTVYSTMLVETAVHDTPMIAATID
                     VPGGWNKPNKFSLSLKEIGDWPTHKRFREAKAGRVTSNEVELRDALNMYLKDRTIDSA
                     ERRKFIENEITFTDATAGKRTAEYILRVLNQASAK"
BASE COUNT          827 a          763 c          771 g          804 t
ORIGIN      
        1 tagaagaaga aatgaaatcg gatatcgttc acgtatggcg cgggataatt cgcgcccgag
       61 gacttctttc aaataatgcc ggttatggag acctgtcagc ggatcgcgga tggcttgttc
      121 gcgcaattca tcctgcagcc cttgaatttc attcatttgt tcttcaagcc tggtataggc
      181 ttcacgcaat tttgcgtcag actctttttg ggcagtgaca tcctctttaa tggcaatgta
      241 attgacaatg atgccatcgc tgtcaatgac aggggcgatc atttccactt cccagtatgt
      301 gtcgccgttc ttctttttgt tcaagagctc accgcgccat attttgccgg attgaatggt
      361 gcgccacatt tcctcatagg cttccagcga ggtatcaccc gattggatga tccgcggatt
      421 cttgccgatc acttcgttta gttcgtagcc ggttagttgc gaaaaatgcg gattgacata
      481 ttctatattc ccttgaatat cggtgattac caccgagacc ggactttgtt ccacggcttt
      541 gtgtaattgc agtagtctta attcggtctg ttttcgctcg gtcacatcgc gcaggatgat
      601 caacctgcca accagccctt ggggccattc atcgtgcaga ggggagatta gtacgtcata
      661 aaagcgctgg tcattggtgc tgagctcaaa cttgatctct ttttctggga tatctttcaa
      721 ctcagaaccg aagcgcggaa aaacatcctc tagatttttc ccaagcaaag catcttctgt
      781 attcccgagc accgctaccg ccgaacggtt gatgtcaatg atgcggttct tcaagtcgag
      841 cgcgatcatg ccgtcactca ggttgttgat taatttgttg cgagccaccg ggacgacatc
      901 cagaaagtta tggccgtaca atgcccagac aaagaggatg ccaatgatcg cagattgaac
      961 cggagctgta tcatatcctt caatctttat tatgtgggta aaagcgcttt caccaatagt
     1021 aaccgtcagt accataataa agagacacag caacatgatc cttgcctgac gtttttggat
     1081 gttggagccc cgtagcgacg cgataacaag taaaacgaga agcggtaatg tgtaacaaag
     1141 agtaccgctg atcagcctgt acccatatgc aggtcctggt acaaactcga gtaaattatt
     1201 ggggtggaga atatactcct tccagaacaa tccatttagc caaggggctg cgcatgtaag
     1261 cgcgatgttg gatatccaat aaaagattaa aatcgctttc acccattttt tctgggcaat
     1321 atgtccgtaa cctgcaaatg tcagcataaa catgagcatt agaggctgac cggttaaata
     1381 cccaaacgat tccagaatcc tgaaaaatat tttccattgc acatccgttg ctgcgtaaag
     1441 aagcacgttg ataaccagcc aaaaaaccga ggtggattgc gcaagggcaa aataatatgc
     1501 ctgtagagaa ttattgcgcc gccagacgac cacggtaaga tatacggaaa ttaccaatgc
     1561 aaagaaagat ataaggacaa aaggggtaat tgtgaacata acacaagttt aatcattttg
     1621 cacataaaat caatctttgg ttctgcgtgt taaaatacgg ttcttatgac caaacgcatt
     1681 ttcatctccg ccgatcacgg catggcaatc atctattttt tacaaagcga cgttgtttcg
     1741 actctgctcg aggcaggtgt ggaagtagtc attcttaccg atgacgatac taaagaaaaa
     1801 atctcacagc gctttgcaaa accgggtctc accttcgaag gcttacgcct gaaacaagcc
     1861 aacgactacg caaaaaaaat cagcccacgc ctgcaatctt tgctcatcta tttgcgccgc
     1921 gtgggcggct cgcgccgcat caacaccgaa gcgatggaca gccatgtgtg ggaagtttgg
     1981 ggcgagaacg gctggaaatt ccgcatcggg gtttggattc cctccgcagt gatgctgttc
     2041 ttcttgcgga atttttcctg ggcacgcaaa cttcttgtgc ggatacaaaa ccgcttcacc
     2101 cccaagccgg gaatttatac cgacctattc gacaaatacc aacccgacat ggtgatcgcc
     2161 tctacccccg gctggcgcat ggaccgttac ctcctgcgtg aatctgccaa acgtggcatc
     2221 ccgaacatga ccgtcatcgt cggctgggac aattcctcct cgtacaacgt cagcggcgca
     2281 gatgtgcagt gggcaacctg ctggtctcaa ttgcaaaaag atgaactggt ttatggttct
     2341 gattggaacc cggaacgagt caacattggc ggcatcccat cgtatgacgg ctacttccgc
     2401 aagcaatggc ttatgccgcg cgatgaatat ttcaaactgc ataacctcga ccccaagcgc
     2461 aagttgattt cctatgccag cagtttcgtc cactttgcgc cgaacttccc gaacattgaa
     2521 gccttggcaa agttggtttc gtctgattca ctggctgaac cttcgcagtt gttgatccgt
     2581 ttgcacccca gccactttca agacaagccg aagatctttg ctgaggaacg tcagcgcgtg
     2641 tttgatttgg aaaagaaata tccgcatgtg catgtggttc agccggttgc tttaggcggc
     2701 tcactcggct actacggcgg cgaagacatg gacgagaaat catccatgat ggcgtactcg
     2761 gatgtgctgg tgaccgtcta ttcgaccatg ctggtggaga cggctgtcca cgatacgccg
     2821 atgatcgccg cgacgattga tgtgccgggc gggtggaata agccgaataa gttttcgtta
     2881 tcgttgaaag aaattgggga ctggcccacg cataagcgct ttagagaggc gaaggcagga
     2941 cgagtaacaa gtaacgaggt tgagttgcgc gacgccttga atatgtattt gaaagaccgc
     3001 accattgact ctgccgaacg acggaaattt attgaaaacg agatcacctt caccgacgcg
     3061 acagcgggaa aacgcacggc ggagtatatt cttcgggtgc tgaatcaggc gtcggctaag
     3121 taggaggcgc ttggtcattt catggtaaaa atgagggctg gagta
//
LOCUS       DAOQNJ010000294         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21734_length_3149_cov_9.678733, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000294 DAOQNJ010000000
VERSION     DAOQNJ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21734_length_3149_cov_9.678733"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..493)
                     /gene="trxA"
                     /locus_tag="PLG52_08860"
     CDS             complement(146..493)
                     /gene="trxA"
                     /locus_tag="PLG52_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNB41598.1"
                     /translation="MSGEPIHITDEAFEQVVMQSNLPVIVDFWAPWCNPCKMIAPTLD
                     KLAKELEGKIVIAKVNTDDHAQWMQKFGIQGIPTLLFVSNGKVVHRQVGALPERMLRD
                     VVNQFMEVAGQQA"
     gene            606..1514
                     /gene="ftsY"
                     /locus_tag="PLG52_08865"
     CDS             606..1514
                     /gene="ftsY"
                     /locus_tag="PLG52_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNB41599.1"
                     /translation="MSIFSRWREGLSRTSKAAFGQIASILGTSEITDETWDDLEATLI
                     QADLGIETASSVLEDLKRLTRAEGWTRSDELFSALKAELRSRLIQPPAISFTDKPTVI
                     LVVGVNGSGKTTTIAKLSAKFVGEGKKILLGAADTFRAAAVDQLQVWGDRLNVEVIAG
                     APESDPGAVAFSAVQAGMARGMDIVMVDTAGRLHTRFNLMEELKKVNRVVGKALPGAP
                     HQVWLVLDATTGQNALQQARSFKEAVNVSGVILTKLDSSARGGMAFAIQRELGLPILF
                     AGLGEKPEDLTPFDPDAFVDGILVNN"
     gene            1523..2612
                     /gene="prfB"
                     /locus_tag="PLG52_08870"
     CDS             join(1523..1588,1590..2612)
                     /gene="prfB"
                     /locus_tag="PLG52_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114679478.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNB41600.1"
                     /translation="MQDLLQRLNAANETVQQLLERLDLVGKQNQLSQLETEIEHPDFW
                     NDSTAAQKVMKTVSSLRDEVEGWTKLKQQVHDLTDLASLEDETLRAELETEIQKVEKE
                     LEKRSFTMMLSGPFDHDDAILAIHAGAGGTDSQDWAGMLQRMYLRWIESRGFTAEILD
                     MTPGEEAGIKSMTIAVSGQYAFGYMRSEKGVHRLVRLSPFDAAHRRHTSFALVEVLPQ
                     VSMDEADVEINQSDVKMDVFRSSGAGGQNVQKNATAVRLTHIPTGIVVTCQNERSQTQ
                     NREFAMRILRARLLQLKQAEKEEERAVLRGEYTKAEWGSQIRSYVLHPYQMVKDHRTD
                     FQVGNAQSVLDGDLDAFMEDYLKSGDGK"
     gene            complement(2662..>3149)
                     /locus_tag="PLG52_08875"
     CDS             complement(2662..>3149)
                     /locus_tag="PLG52_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNB41601.1"
                     /translation="DMVVKGKVTRLETYGAFVDFGAERPGLIHVSELAHGYVKTAGDV
                     VKAGDEVEAKVLEVDRRKRQIRLSIKALQELAVEEAAEPVEYKGKGKRKGKKQEESYE
                     METEVSNEPQYTAMQIAWQQALDKANGKKNIRAKSIKGTSKEQENLYNSTLEKRLPTG
                     G"
BASE COUNT          795 a          716 c          804 g          834 t
ORIGIN      
        1 cagccgtagc ttgtaggtct gtttcagaaa caggggtagg gtattcttga ggctgcgaaa
       61 gtgatgggag acaggcactt aataacaaac ccaacacaat agaaacaaga attagcctct
      121 tcatacccta ctccaacgtt ttaatttagg cttgctgtcc cgcaacttcc atgaattggt
      181 tgaccacatc gcgcaacatg cgttcgggga gtgcgccaac ttggcggtga acaaccttgc
      241 catttgaaac aaacaacaaa gttggaatac cttgaatgcc aaacttctgc atccattgag
      301 catgatcatc tgtgttgact tttgcaataa cgatcttgcc ttctaattct ttggcaagtt
      361 tatcgagagt aggcgcgatc atcttacaag ggttgcacca tggtgcccaa aaatccacaa
      421 taacaggaag attggactgc ataacaacct gttcaaacgc ttcgtcagtg atatgaatgg
      481 gttctccgga catttttatt cctttttaac ctttatgatt ctttcttatt gagccatatt
      541 tagccaaatg agtataacat aaaaaacatg ccatcatatg cccatagggt ataatcgccg
      601 cccatatgag cattttctca cgatggcgag aaggactttc tcgcacaagc aaagctgcat
      661 ttggtcagat cgcttccatc cttggtactt ctgaaattac agatgaaaca tgggatgacc
      721 ttgaagctac attgattcaa gcagacttag gaattgaaac agcatcatct gtgctagagg
      781 acttaaaacg cctcacgcgc gccgaaggct ggacacgctc cgacgaactt ttttctgcgc
      841 tgaaagctga actgcgttct cgcttaattc aaccgcctgc tatttccttc acagataaac
      901 caactgtgat tttggtagtg ggtgttaacg gttctggcaa gacgacaacc attgccaaac
      961 tttctgccaa attcgttggc gaagggaaaa agatacttct tggcgcagcg gacaccttcc
     1021 gggctgcggc ggtggaccag cttcaggtgt ggggagacag gctaaacgtt gaagtgatcg
     1081 ctggcgctcc agagagtgac ccaggtgcgg tggcgtttag cgccgtccag gctggtatgg
     1141 ctcgcggcat ggatatcgtc atggtcgata ctgccggacg cttacatacc cgcttcaacc
     1201 tgatggaaga gttgaagaag gtgaaccgtg tagtgggtaa agcattgccc ggtgcgccgc
     1261 atcaagtgtg gcttgtgtta gatgccacaa cagggcagaa tgctttgcaa caggcgcgtt
     1321 cttttaagga agccgtgaat gtctctggcg tcattttgac gaaattggac tcttctgctc
     1381 gcggcgggat ggcgtttgcc attcagcgtg aattagggtt gccgattttg tttgcaggct
     1441 tgggagaaaa acccgaagac ctgaccccgt ttgaccccga tgcctttgta gatggaattt
     1501 tggtaaataa ctaggagtaa agatgcaaga cttattacaa cgcttgaatg ctgccaacga
     1561 aaccgtacaa cagttattgg agcgtctttg acctggtagg aaaacaaaat caactttcgc
     1621 aattagaaac tgagatcgaa cacccagact tctggaatga ctctactgct gcacagaagg
     1681 tcatgaagac cgtttcaagt ttgcgtgatg aagtggaagg gtggaccaaa ctcaagcagc
     1741 aagttcatga tttgacagac cttgcctcac tggaggacga gactcttcgt gcagagttgg
     1801 agaccgaaat tcagaaggtt gagaaagaac tggaaaagcg ctctttcacc atgatgcttt
     1861 cgggtccgtt tgaccatgac gatgcgattc tcgcaattca tgctggcgct ggtggaacag
     1921 actcacaaga ttgggctggg atgcttcaac gtatgtatct gcgttggatc gaaagccgtg
     1981 gcttcaccgc cgaaatttta gatatgactc ccggtgaaga agcaggaatc aaaagcatga
     2041 ccatcgctgt aagcggacaa tatgctttcg ggtatatgcg ttctgaaaag ggagttcatc
     2101 gcctggtgcg gctttctcct tttgacgcag ctcatcgccg acatacatcc tttgcgttgg
     2161 ttgaagtgtt gccgcaagtc agcatggacg aggcagatgt ggagatcaac cagagcgatg
     2221 taaaaatgga tgtcttccgt tcctctggtg caggcggaca gaatgtgcag aaaaatgcca
     2281 ccgctgtgcg cttaacacat attccaacag gaattgtagt gacttgtcag aatgaacgtt
     2341 cacagacgca gaaccgcgag tttgccatgc gaattctccg tgcgcgtttg cttcagttaa
     2401 agcaagcaga gaaagaagaa gaacgcgctg tgttgcgcgg agaatacacc aaggcagaat
     2461 ggggcagcca aattcgttcg tatgtcttgc atccatacca aatggtgaaa gaccatcgca
     2521 ccgattttca ggttggcaat gcgcagtctg tgctagatgg cgatttggat gcgtttatgg
     2581 aagactattt aaagagtggg gatgggaagt aaattggtac ataaaaagag cggacatttg
     2641 tccgctcttt tttgtttttc tttagccgcc agtggggagg cgtttttcta aggtgctgtt
     2701 gtaaagattt tcctgctctt tggaagtacc cttgatggat ttggcgcgga tgttcttctt
     2761 gccgtttgcc ttgtccaacg cttgctgcca agcgatttgc atggcagtgt actgcggttc
     2821 gttggaaact tccgtttcca tttcgtagga ttcttcttgt ttcttgccct tgcgcttgcc
     2881 tttgcccttg tattcaacgg gttcggcggc ttcttcaacc gcaagttcct gaagagcctt
     2941 gatgctcaag cggatctggc gcttgcggcg atctacttcg agaaccttgg cttccacttc
     3001 atcgccagct ttcacaacgt cgccagcagt cttgacatag ccgtgagcca attcgctaac
     3061 gtgaatcaag ccggggcgtt ctgcgccgaa gtctacaaaa gcgccgtagg tttcgaggcg
     3121 ggttactttg cctttcacaa ccatatcgg
//
LOCUS       DAOQNJ010000295         3148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21747_length_3148_cov_9.399289, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000295 DAOQNJ010000000
VERSION     DAOQNJ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21747_length_3148_cov_9.399289"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLG52_08880"
     CDS             <1..534
                     /locus_tag="PLG52_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41602.1"
                     /translation="NAEVANCRFGPGTVYQTMNEVRGGRLLNAVGRDAASTWWQVEDP
                     INPGGYCWVAAEVTTLQGTAEQLPIVDAPFVSVTKVDLRVEPNRIVVNCNDFPQTVFF
                     EANITANGPTLLNWQWEASTGVTSDVGTLIFEESGTQVINEFYRINAPNEYWVKLKIL
                     SPNQMEEQVTFPVSCNP"
     gene            complement(579..1250)
                     /locus_tag="PLG52_08885"
     CDS             complement(579..1250)
                     /locus_tag="PLG52_08885"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNB41603.1"
                     /translation="MKNTFNTIMSILILIVLAAGVYFIVQTVRETATAAQQPFEQVNQ
                     ANQALQTQVAQLANPTPTIIPDPVTYINEIRALARLETIQYSISQVMTGELNQGTFGF
                     AFGTKILVVVHGTVIAGIDMEKILPEDMRYENGVLYVRLPPAEVLATTLDTQKTEVYT
                     LQEGLLVDIDPNLVLSTIQGGQEKILSVAIEDGILQQAERNAQAYLIKFFNALGFNNV
                     IFSEE"
     gene            1295..2335
                     /locus_tag="PLG52_08890"
     CDS             1295..2335
                     /locus_tag="PLG52_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNB41604.1"
                     /translation="MMFKTKQSCKTNKILLTITLLFTLGCSFPLQVTFGTPTATPTLT
                     PTPAATQAPLSTPEPGTEQNPLILALAPNPHPNDAIISAGDTLAAFIEKQTGYRVVTV
                     IPASEEALVNDLAKGNAHIASLTPYAFVLAHENGSVNALLARVRNGEMFYGAQILINR
                     ENVFTAYYDEERAENTTDALSALTQFADKKPCWSDETSPSGYVVPLGLLNQYQVQTRA
                     GAFLEGQPNVVRGVYAADICDFGATFVDARNSPILEADYPDVMDKVRVAWRIPEIIPY
                     ENISMAASLPFEMRRSVQRSLIDFMVTADGKAVMQTIYGFDEMLPIEDASYAEFIKYV
                     NASGLNLLDLIE"
     gene            2340..2987
                     /locus_tag="PLG52_08895"
     CDS             2340..2987
                     /locus_tag="PLG52_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNB41605.1"
                     /translation="MFKRVIRFLWRTLLVLSILIAIGLFMPRWIMALFAAPRTFTVED
                     VPATNVVIVFGAGLLRDGSAGPVLSDRMLTAVNLYHAGKVEKILVSGDNRFIEYNEPE
                     AGRQYALEHGVPDEDIVLDYAGRRTYDTCYRAKHIFGVNEAILVTQDFHMPRALALCN
                     WFGLNAVGVESNNRYFLKRSQVWWNFRETFAVFQAAWDLIVTKPLPVMGEPEPIN"
BASE COUNT          809 a          767 c          723 g          849 t
ORIGIN      
        1 aacgcagaag ttgccaactg ccgttttggt cccggcacgg tctaccaaac catgaatgaa
       61 gtccgcggtg ggagactctt aaacgctgtc gggcgcgacg cggcatctac ctggtggcag
      121 gtcgaagatc ccatcaaccc aggcggatat tgctgggttg cagccgaagt tacaaccctt
      181 caaggaaccg cagagcaact acctattgta gatgccccct ttgtcagcgt caccaaagtg
      241 gatttgcgcg tggagccgaa tcgtatcgtc gtcaactgca acgactttcc gcagaccgtc
      301 ttctttgaag ccaacatcac cgccaacgga ccaaccctcc ttaattggca atgggaagcc
      361 agcacaggcg tcacctcaga tgtaggcaca ttgatctttg aagagtctgg cacacaagtc
      421 atcaacgagt tttaccgcat caacgcgcct aatgagtatt gggtaaaact aaaaatcctt
      481 tcacccaatc aaatggaaga gcaagtgacc ttccccgtta gctgtaatcc ttaaataaaa
      541 aaggcgactc acatgagtcg ccttttttat tgttttcttt attcttcaga gaaaattaca
      601 ttattaaagc ccaacgcatt aaagaatttg atcaaatacg cctgagcatt tcgttctgct
      661 tgctgcaaaa ttccatcttc gatggcaacg cttagaattt tctcctgccc accttgaatt
      721 gtggatagca caaggtttgg gtcaatatct actaataaac cttcttgcaa ggtatacact
      781 tcggtctttt gagtatccaa cgtggtggct aatacttctg caggcggcag gcgcacatat
      841 aaaacgccat tctcatagcg catatcttca ggcagaatct tttccatatc aatgcctgcg
      901 atcaccgttc catgcaccac cacaagaatc tttgttccaa aagcaaaacc aaacgtacct
      961 tgattcaact cgccggtcat cacttgtgaa atactgtatt gaatcgtctc caaacgagca
     1021 agcgcacgaa tctcattgat gtacgtcacc gggtctggga tgatggttgg ggtggggttc
     1081 gccaattgtg ccacctgagt ctgcaatgcc tggttcgcct gattcacctg ctcaaagggc
     1141 tgttgcgccg cagtagctgt ctcgcgcacg gtctgtacga taaaatagac cccggcagca
     1201 agcacaatca aaatcaggat ggacataatt gtgttgaacg tatttttcat tttcttcctt
     1261 tcatttcatc ttggtcaagt attatagcaa ggtcatgatg ttcaaaacca aacaatcctg
     1321 taagactaac aagattctat taaccataac actattgttt acgcttggat gttctttccc
     1381 gttgcaagtg acctttggga ctcccactgc gactcctacc ctcacgccaa ctcctgctgc
     1441 cacacaagcg cctctttcca cgcctgagcc gggcactgaa caaaatccat tgatcctcgc
     1501 cttagcccca aatccacatc ccaacgatgc catcatctct gccggagata cactcgccgc
     1561 ctttatcgaa aagcagaccg gttatcgcgt tgtgactgtc atcccggcat cagaagaagc
     1621 gttggtaaat gatttagcaa aaggtaatgc gcatattgca tcccttacgc cgtatgcctt
     1681 tgtgcttgcg catgagaacg gatcggtcaa cgccttactg gcaagagtcc gcaacggaga
     1741 gatgttctat ggcgcgcaga ttcttatcaa tcgtgagaat gtatttacag cctattacga
     1801 cgaagaacgg gcagagaaca ctaccgatgc gctctctgct ttgacgcaat ttgccgataa
     1861 aaaaccatgc tggagcgatg aaacatcccc atctgggtat gtggtgccgc tcgggttgct
     1921 gaatcagtat caggtccaga ccagagccgg tgcttttctt gaaggtcaac ccaatgtggt
     1981 gcgtggagtc tacgctgcag atatctgtga cttcggcgca accttcgtcg atgcacgcaa
     2041 ctcgcccatt ctcgaagcag attaccccga cgtgatggat aaggttcgag tggcgtggcg
     2101 catcccagag atcattccat atgaaaatat ttcaatggct gcttctttgc cttttgaaat
     2161 gcgccgttct gtgcaacgtt ctttgatcga ttttatggtc acagccgatg gcaaagcggt
     2221 tatgcaaact atttatggct tcgacgaaat gctgcctatt gaagatgctt cgtatgcgga
     2281 gttcatcaag tacgtgaatg cctcgggctt gaatcttttg gatttgatag aataaaacga
     2341 tgtttaaaag agtaatccgt ttcctttggc ggactctgct tgtgctgagt attctcatcg
     2401 ccattggatt gtttatgccc agatggatta tggcattatt cgctgctcca cgaaccttca
     2461 cagtggaaga tgtacctgca accaatgttg tgattgtgtt cggcgccgga ctcttgcgcg
     2521 atggctctgc cggaccggta ctcagtgacc gaatgttgac tgccgttaat ttgtatcatg
     2581 ctggcaaagt agaaaaaatc ttggtcagtg gtgacaatcg ctttattgag tacaacgagc
     2641 cggaagcggg tcgtcaatat gctttggagc atggtgtacc tgatgaagac attgtgctcg
     2701 attatgcagg taggcgtacc tacgatactt gttatcgtgc caagcatatc ttcggcgtga
     2761 atgaagccat tctcgtcaca caagattttc acatgccgcg cgcattggcg ctttgtaatt
     2821 ggtttggtct taatgcagta ggcgtagaat cgaataaccg ttactttcta aaacgttcgc
     2881 aggtttggtg gaatttccgt gagacctttg cggtctttca agccgcatgg gatttgattg
     2941 tcaccaaacc gttaccagta atgggtgaac cagaacctat aaactaatat cacattgagt
     3001 gacaaccaaa ggagaactct atgaatcgcg aagaagcttt agcccttgta cgtgaatatg
     3061 tcaaaagcga aagtttggta cggcatatgt tatcggtaga agccgccatg cgtttctatg
     3121 ccgaaaagtt tggggaggat gttgaatc
//
LOCUS       DAOQNJ010000296         3147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21763_length_3147_cov_10.649418, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000296 DAOQNJ010000000
VERSION     DAOQNJ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21763_length_3147_cov_10.649418"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1251
                     /locus_tag="PLG52_08900"
     CDS             373..1251
                     /locus_tag="PLG52_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNB41606.1"
                     /translation="MTMKRIIVAGIYRSGTSLITELVHRWGAYAGREEDIFKDEFGYM
                     EHFALQKLNDELLNNNSRVPTPVETLLEKAEDAGLRERALSILAEMDEEAQANHAAAW
                     VWKDPRLPLVLPFWADVWGDVIYVIPVRHPVETIFSAAKMDGLDPEQLPLSAGLAYWQ
                     FCMLNLLQYTANTPRKLFMAYDQLTKDPQRECERLCRFLDAQCEVDSDPSDRIRSMTE
                     QVAAGERHFNHPRSLAEVETSTREQRALYDFLRVKTLYPDEPFHKDDFAMYPGWFEYL
                     SAMDMLFSAGDAGQEA"
     gene            1255..2169
                     /locus_tag="PLG52_08905"
     CDS             1255..2169
                     /locus_tag="PLG52_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41607.1"
                     /translation="MSPMSLEAFLALPTEELANLVRGDRVKTCVFPFNGTRRWFLLER
                     PSSAGGDLAKQYIELTTQGYINTYKLVFDHGIDNIVAPAFGGELLVNRGEEYMRQIGT
                     SLSLLAIHPDFVNFYKEYDVRVHFYGEYRKQFKDTSYAYIIDQFDEVTRQTADHTSRA
                     LFYGVFAEDATMTTAELAIQHVQQTGTHPTRRDLIERYYGEFIEKADIFIGYEKFNVF
                     DYPMLGLGEESLYFTVAPSLYMNDRQLRNILYDFLYLRPVQDPDYFSMPQADFEAMRR
                     YYEANREVTFGIGEMRGGIWYATPHLKK"
     gene            2178..>3147
                     /locus_tag="PLG52_08910"
     CDS             2178..>3147
                     /locus_tag="PLG52_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41608.1"
                     /translation="MLNIDEEVKKVLNEVGPGRMSSTAYDTAWVARLGEIDKDLSDQA
                     VNWLLENQLPDGSWGASDVFYYHDRVISTLIAMIALKEHGGNSHHQAQVEKGVEALER
                     ITSDGEHSFASDLNGATVAFEMVAPILLAEAKELGIIKQQGERILGRLSNMRQEKMRK
                     LAGYKINRYVTPAFSAEMAGIDGQHMLEVENLQEANGSIGHSPSATAYFALRVKPGDA
                     KAIDYIKSSVAKNGGAPDLYPFDVYERAWVLWNLSLTDYFEGDKRSFANPILNYLAAA
                     WKPGQGVGFSKDYSVIDGDDTIITCELFNKFGYPTDLNAVLSFEEEE"
BASE COUNT          826 a          727 c          771 g          823 t
ORIGIN      
        1 acctgtaaaa taattaatgt aacaagcatt gaatcaaggt tctatacaac gttgcttaac
       61 cgcccttcaa cgtcactcct gcaatatacg tgggatgcaa gactgtaagc ccttctattt
      121 aatagcatat ccagagggga aattgaccgg gcgcacaaaa tccaaaaaga gccgcttctt
      181 actccaccca tgcttgggta agcctttctg agagccatcc cttcgccgaa acattttgca
      241 tttgagtcta tgggttgtgc ctaaagatgg gggattgttg tatatgaatt cctgaagaga
      301 tcagcgttct taattttgtt catgccgtag aagagttgtt aactttatac tttttgacac
      361 ataaaaggac aaatgacaat gaagcgtatc atcgttgccg gaatttatcg aagtgggaca
      421 tcactaatca cagagttggt ccatcgttgg ggggcgtatg cagggcggga agaagatatt
      481 ttcaaagacg aatttggcta tatggagcac ttcgccttac agaaattgaa cgatgaactt
      541 ctaaacaata acagccgcgt tcccacacct gtagagacct tgcttgaaaa agcagaagat
      601 gccgggttgc gagaacgtgc cttgagcatc ttagctgaaa tggatgaaga ggcgcaggcg
      661 aatcatgccg cggcatgggt ttggaaagac ccacgcttgc ctttggtgct tcctttttgg
      721 gcagatgtat ggggtgatgt tatctatgta attcccgtgc gtcacccagt agagacgatc
      781 ttctccgccg ccaaaatgga tgggctcgac ccggagcaac ttccgctttc agctggcttg
      841 gcatactggc agttttgcat gttgaacttg ctccaataca cagccaatac ccctcgcaaa
      901 ctattcatgg cgtacgacca acttacgaaa gacccacagc gtgaatgtga acgcctgtgc
      961 cgttttctcg atgcgcaatg tgaagtcgat agcgacccat cggaccgtat tcgttccatg
     1021 accgaacagg tcgcagcggg ggagcgccac tttaaccatc cacgctctct ggcagaagtg
     1081 gaaacatcca cccgtgagca acgggcgtta tatgatttct tgcgcgtcaa aaccctttac
     1141 cccgatgaac ctttccacaa agacgatttc gccatgtacc ccggttggtt tgagtatctc
     1201 tctgccatgg atatgctctt ctctgccggg gatgctgggc aggaggcata agaaatgtct
     1261 cctatgtctc ttgaagcttt tcttgccctg cctaccgaag agttggcaaa ccttgtccgc
     1321 ggcgataggg taaaaacctg tgtctttcct tttaatggca cgcgccgctg gtttttgtta
     1381 gagcgtccat catctgctgg cggcgatctt gccaaacaat acatcgagtt gaccacgcaa
     1441 ggatatatca atacctataa attggtcttt gaccacggca tagataatat cgtcgccccg
     1501 gcttttggcg gtgagttatt ggtcaatcgc ggcgaagaat acatgcgcca gatcggaacc
     1561 agcctgtctt tgctggcaat tcacccagac tttgtcaact tctataaaga atatgatgta
     1621 cgcgtccact tttatggaga gtatcgcaag cagtttaagg atacttctta tgcctacatc
     1681 attgatcaat tcgatgaggt cacacggcag acggcagatc atacgtctcg cgccttgttc
     1741 tatggggtct ttgccgaaga tgccaccatg acaaccgcag agcttgccat tcaacacgtc
     1801 caacaaacgg gtacgcaccc cacccggcgc gacctgatcg agcgttatta cggcgaattc
     1861 attgaaaaag cggatatttt catcggctac gaaaaattca acgtcttcga ctatcccatg
     1921 cttggcttgg gagaagagag cttgtacttt acggtggctc cttctctata tatgaacgac
     1981 cgtcagttga gaaatattct ctatgatttt ctttatctgc gtccggtgca agaccctgat
     2041 tatttcagca tgccccaagc agattttgaa gccatgcgca gatattatga agcgaaccgc
     2101 gaggtcacct ttggaatagg tgagatgcgc ggaggaattt ggtacgccac cccacacctt
     2161 aaaaagtaga gttcatcatg cttaacatcg acgaagaagt aaaaaaagta ctgaacgagg
     2221 tgggacccgg acgaatgagt agcactgcct acgacaccgc ttgggtcgca cgcttaggcg
     2281 aaattgataa agacttaagc gaccaagctg tcaactggct gcttgagaat caattgccag
     2341 acggtagttg gggcgccagc gacgtgttct attatcacga ccgtgtcatt agcacgctga
     2401 ttgccatgat tgcgcttaag gaacatggtg ggaattctca tcatcaagct caagttgaaa
     2461 agggtgtaga agcattagag cgtatcactt ctgatggaga gcacagcttc gcttcagacc
     2521 taaacggtgc cacagttgct tttgaaatgg tcgctccgat cttgcttgca gaagccaagg
     2581 agctaggcat tatcaagcaa cagggagagc gaattctagg tcgcctgagc aacatgcggc
     2641 aagagaagat gcgcaaactt gccgggtata agatcaaccg ctatgtcact ccagcctttt
     2701 cggctgagat ggctggtata gatggtcaac atatgcttga ggttgagaac ttacaggaag
     2761 cgaatggttc cattgggcat agcccatctg ccacagctta ttttgctttg cgggtaaaac
     2821 ccggagatgc aaaagcgata gattatataa agagctctgt ggcgaaaaac ggcggcgcgc
     2881 ctgatctcta tccgtttgat gtgtatgaac gggcatgggt cttatggaat ttatctctaa
     2941 ccgattattt cgaaggcgat aagcggtctt ttgctaaccc tattttgaac taccttgcag
     3001 cagcatggaa accggggcag ggggttggtt tttctaagga ttacagtgtt attgatggcg
     3061 atgacaccat cattacttgt gagttgttta ataaatttgg ctatccaacc gatcttaatg
     3121 ccgtattgtc ttttgaagaa gaagagt
//
LOCUS       DAOQNJ010000297         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21778_length_3146_cov_9.581042, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000297 DAOQNJ010000000
VERSION     DAOQNJ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21778_length_3146_cov_9.581042"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLG52_08915"
     CDS             <1..344
                     /locus_tag="PLG52_08915"
                     /inference="COORDINATES: protein motif:HMM:NF022517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HNB41609.1"
                     /translation="AEKIMNVIKGFDKDGDWLFANWLWKLRGGLESKRSDFYEVDVQN
                     ENHILFRSKLKSPGEGWMEWRVEEGLLIQTAYFAPRGLGGFLYWYLLAPFHLYLFRGL
                     IKSIIQKAMVQ"
     regulatory      344..493
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            575..1546
                     /locus_tag="PLG52_08920"
     CDS             575..1546
                     /locus_tag="PLG52_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNB41610.1"
                     /translation="MFRKNLILTLLIALVAACAPAPQPTATVVPVSTEISVTDVVATE
                     AVAEGLSFTDGLGREVTLAAPAQRIVSLAPSNTEILFAVGAGDQVIGRDEFSDYPAEA
                     TSIESVGGSMGEYSAEAIVALEPDLVLAAEINTPELVSQLEDLGLTVYYLSNPTTIEE
                     MYTNLEIVGQLTGHDTTEMVDSLKARVAAVDEKIAPISSRPSVFYEIDASDPSKPWTY
                     GPGSFGNLLIERAGGFNIASDAPDSYPQLSLEQVVVANPSVILLGDSMWGVTPESVLD
                     REGWNTILAVQNNQIFPIDDNLISRPGPRLVDGLEALAKILHPGLFE"
     gene            1647..2642
                     /locus_tag="PLG52_08925"
     CDS             1647..2642
                     /locus_tag="PLG52_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNB41611.1"
                     /translation="MMKKPFLSSLLFLLFAIVLSLAIGSVFISPTELWKILRGVGEEK
                     FAFIIWNIRLPRTVLIALTGAALSGSGASYQGLFRNPLADPFLIGVASGAGLGAVIAM
                     SIQWPYSFWGLMAIPAVAFISALLTVFIVYFLARVGQTIPTTNLILAGVAFSSFATSL
                     TSFLMLRSTNEVRRALGWLLGGASQAGWTAILAMLPYLVIGLGILIFFGYQLNLLQFG
                     DDQAQQMGLNVNRTKTILLIASSLATAAAVAFSGIIGFIGLVVPHIMRLWFGADYRRL
                     IPLSIISGASALLFSDIIARIVLAPQEIPVGIITALAGAPFFLWVLRRVKNQGYW"
     gene            2647..>3146
                     /locus_tag="PLG52_08930"
     CDS             2647..>3146
                     /locus_tag="PLG52_08930"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB41612.1"
                     /translation="MTVTTYKLDASHLHLFNQFDRTFSVSSKVLLDMTGGRLTWGIIP
                     IEPYEKEIPIDEVDPATFINSEDKVVFLAEVDGKLAGQIKLITWWNRYAYIDDVIVNP
                     QFRGQGVGKALMEAAIQWSREKRFPGIMLETQDDNVAACKLYDSCGFVLGGFDQYTLK
                     NFKPNET"
BASE COUNT          737 a          809 c          733 g          867 t
ORIGIN      
        1 ctgcggaaaa aatcatgaac gtcatcaaag gcttcgataa agatggagac tggctgtttg
       61 ccaattggtt atggaaattg cgcggtgggc tcgaaagcaa gcgcagtgac ttttacgaag
      121 tggatgttca aaacgagaat catattttgt ttcgttcaaa actaaaatcg cccggcgaag
      181 gttggatgga atggcgtgtg gaagaaggtc ttctcattca aacagcatac tttgccccac
      241 gcggactcgg cggattttta tattggtatt tgcttgcgcc atttcatctt tatctttttc
      301 gcggtttaat caagagcata atccaaaaag caatggtaca atgacctcaa cataacgaat
      361 cagcaagcgg cgaagtccgg tgcaaacccg gcgctgtcgc gcaactgtga agttcgaaat
      421 ttttattttc gaacaaagcc aggtcgcccg ctctgattcg gttcaaccac ttctcgcgga
      481 aaggaggtgg agaacaggtc cagcccggat tgactctccc acctccccag cctatgctgg
      541 ggattttgat tccatacaca caataggaga aaagatgttt cgtaagaatt tgattttgac
      601 tttactgatc gcgctcgttg ccgcatgtgc ccctgcgcct caacctacag cgacagttgt
      661 tcccgtttcg acagaaattt ctgtcactga tgtcgtcgcc accgaagctg ttgctgaagg
      721 cttatccttc accgatggat taggtcgcga agtgactctc gctgcgcctg cccagcgcat
      781 tgtttcactt gcaccttcca acacagaaat cctctttgcc gtcggcgccg gtgaccaagt
      841 cattggtcgc gacgaatttt ccgattaccc tgctgaagcc acttccatcg aaagtgtagg
      901 cggttccatg ggcgaatata gtgccgaagc catcgttgct ctcgaacccg atctggtgct
      961 cgccgctgag atcaatacgc ctgaactcgt cagccagtta gaagacttgg gtctcaccgt
     1021 ttattatttg agcaatccca ctacgatcga agaaatgtac accaatcttg agatcgtcgg
     1081 tcaacttact ggacatgaca ccaccgaaat ggtcgattcg ctcaaggctc gcgttgccgc
     1141 tgtggatgag aaaatcgcgc ctattagctc acgcccatcc gtattctatg aaattgatgc
     1201 ctctgacccc tccaagcctt ggacgtatgg tcccggttcc ttcggcaatt tattgatcga
     1261 acgtgccggc gggttcaaca ttgccagcga tgcgcctgac tcctatcccc aattaagttt
     1321 ggaacaagtt gtcgtcgcga atccttccgt cattttgctt ggagattcca tgtggggtgt
     1381 tacgcctgaa tccgtgctcg atcgcgaagg ttggaacact atccttgccg ttcaaaacaa
     1441 ccagatcttc cccatcgacg ataacctcat cagccgcccc ggtccgcgcc tggtagatgg
     1501 gcttgaagca ttggcaaaga ttttgcaccc cggtctattt gagtaattga ccaaacgtgt
     1561 gtcgcaccaa atgggtagat aagtctcctt acatcagcaa aactacacgg taaacttatc
     1621 ctatcctttg gtgcgatgca ctcgttatga tgaaaaaacc ttttctttct tctttgcttt
     1681 tccttttatt cgccattgtg ctcagtctcg ccattggctc ggttttcatt tccccaacgg
     1741 agttatggaa gattctgcgc ggtgttggag aagaaaaatt tgccttcatc atttggaaca
     1801 tccgcttacc acgcacagtg ctaatcgctc ttaccggtgc cgccctaagc ggaagcggcg
     1861 catcatacca agggttattt cgtaacccgc tcgcagaccc attcttgatt ggcgtggcat
     1921 ctggcgcagg gctgggggcg gtgattgcca tgtctattca gtggccgtat tcgttttggg
     1981 ggctcatggc aattcctgcc gttgcattta tctcggcatt gcttacagta tttattgttt
     2041 attttcttgc ccgggttggt caaaccattc ccaccacaaa tttgattcta gccggggttg
     2101 ccttctcttc tttcgctaca tcactcacct cctttctaat gctgcgctcc accaacgaag
     2161 ttcgccgcgc actgggatgg ttgcttggag gagctagtca agccgggtgg acggctatcc
     2221 tcgccatgct gccatatctt gtcattggtc tggggattct gattttcttc gggtaccaac
     2281 tcaacctttt gcaattcggc gatgaccaag ctcagcaaat ggggctgaac gtcaaccgca
     2341 caaaaactat tttgctaatc gcatcgtcgc ttgcaaccgc agcagcagtg gcattctctg
     2401 gcatcatcgg ctttatcggt ctggtggtgc cacatattat gcgcctgtgg ttcggcgcag
     2461 attatcgaag actcattccg ctctctatta tcagcggcgc atctgcgctt ttattttcag
     2521 atatcatcgc ccgcattgtg cttgctccgc aagagattcc ggtaggcatc atcactgcat
     2581 tggctggcgc acctttcttt ttgtgggttc tccgccgtgt aaaaaatcaa gggtattggt
     2641 aaagacatga cagtcactac atataaactt gatgcatctc acctgcatct tttcaaccaa
     2701 tttgatcgaa ccttttccgt ctcatcaaag gtcttactag atatgaccgg tggcagatta
     2761 acctggggca tcatacctat agaaccatat gaaaaagaaa tccccatcga tgaggttgac
     2821 cctgcaactt ttatcaacag tgaagataaa gtggtcttcc ttgcggaagt ggatggcaaa
     2881 cttgccgggc aaatcaagct catcacatgg tggaacagat atgcctatat tgatgatgta
     2941 attgtcaacc cgcagttcag aggtcaaggc gtaggcaaag ccctgatgga ggctgccatt
     3001 caatggtcaa gagagaagcg ttttcccggc atcatgctcg aaactcaaga tgataacgtc
     3061 gccgcgtgca agctatacga ttcttgtggc tttgtcctcg gcggcttcga ccaatacacg
     3121 ttgaaaaatt tcaagccaaa tgaaac
//
LOCUS       DAOQNJ010000298         3140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21828_length_3140_cov_11.040194, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000298 DAOQNJ010000000
VERSION     DAOQNJ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21828_length_3140_cov_11.040194"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="PLG52_08935"
     CDS             <1..1032
                     /locus_tag="PLG52_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41613.1"
                     /translation="GTKKGLEMNIKTLIPAASIFLFACLALTPPTEEPAPPTEETTAP
                     ENTTIVPEQPIGNGPSIENCPMFPENNVWNTPIDSLPLHPLSDAWIESIGSAEPIHMD
                     FGSGTWDGGLIGIPFNIVAGSAVPTFEPGFYYPEESDAGPYPIPDNPNIEYGSDHHIL
                     VVDTETCTLYEIFDASFDGSNWNGGGGAIWDLNSNALRPDTWTSADAAGLPILPGLVR
                     YDEIVAGEIKHALRFTAEQTAGYLWPGRHLTADPDDSIPPLGARFRLKADYDISGFPP
                     EMQVILQALKTYGMFLADNGSDWYVSGAPDERWDNDMLHLLDVLVGSDFEVVDASGMI
                     VDVDSGEAK"
     gene            1067..1405
                     /locus_tag="PLG52_08940"
     CDS             1067..1405
                     /locus_tag="PLG52_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41614.1"
                     /translation="MSNSVHETIPQNRKGRWGRFGLAISILSQVVLLCSIISFFSSFQ
                     FMEGMFVPILFVSLPLAVIAIFLIVIGLVLCIVGIFTQDGSRKTALIGLGVLSFTFVF
                     LLIAYLRLNP"
     gene            1447..2781
                     /locus_tag="PLG52_08945"
     CDS             1447..2781
                     /locus_tag="PLG52_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41615.1"
                     /translation="MTIPQPSTNDTKNKKKYFTTLAFIFNAMGILLALLIIPAVYLSY
                     IPFAILLTAPAMLIIGVLAIILWAIGLYQGIKAIRSEETRKIGAWVATLLNGFLIIGA
                     TCSFIVATSLIFSPTTSIFSTYYSGAGFSPDGNVVAVGYKNKILLLDSKTGEKIKTLG
                     GNKNATDLIIFSPDGNLLADSSYGRLTIRDMTTYKTLKTFTKENDRVFDFYFSPDGKL
                     ITIIWFNKGAEVISTSTFEVLKTVSFGNNFDTVYNKYISETSHHFGKTDNSEENLSVW
                     EMNDFIENSLTKPIISIPLPQSSENTFVYFDRYDVTPDGSLLVVPIPTSIDGDYNKSE
                     FILWNITTAELIATLEFNPVIGIGGYDSHPDPEYYFAFSPDSQTLAYINEKNNITLID
                     LNTFQTRDIDVEDEYVARYLAYNHDGSQILITNAKSIQMISVDSGNIVWTYP"
     gene            2789..>3140
                     /locus_tag="PLG52_08950"
     CDS             2789..>3140
                     /locus_tag="PLG52_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB41616.1"
                     /translation="MNSFTPRPSQQKILRYNGGRLGIAAVPGAGKTHILSALAAKLIQ
                     EGRLGDDQEVLIVTLVNSAVDNFETRIKRFFDNPLQALYKYRVRTLHGLAHDIVREKP
                     AKVGLEERFSIIDER"
BASE COUNT          848 a          719 c          693 g          880 t
ORIGIN      
        1 ggtaccaaga aaggtttaga aatgaatatc aaaactttaa taccagccgc ttcgatcttt
       61 ctttttgcat gtcttgccct cacaccgccc actgaagaac ctgctccgcc tacagaagaa
      121 accactgccc cagaaaatac aactatcgtc cctgaacaac ccataggaaa tggtccaagc
      181 attgagaatt gcccaatgtt ccctgagaat aatgtatgga acacgccgat cgattcgctt
      241 ccgcttcacc cgttgtctga tgcgtggatc gagagcattg gctcagccga acccattcac
      301 atggacttcg gctcagggac gtgggatgga ggtttgattg gaatcccatt caatatcgtg
      361 gcagggtcag ccgtcccgac gtttgagccg gggttttatt atcctgaaga atcagatgca
      421 ggtccgtacc ccatccctga taatccaaac atcgagtatg gctccgacca tcatattctg
      481 gttgtagata cagagacctg caccttgtat gagatctttg acgccagctt cgacggctca
      541 aattggaatg gcggcggcgg cgcgatctgg gacttgaatt caaatgccct gcgccccgat
      601 acatggacct ctgccgacgc ggcggggttg ccaattttgc cgggcttagt gcgctatgac
      661 gaaattgtag caggcgagat caaacacgcc ctgcgtttca ctgccgaaca gaccgctggc
      721 tatctctggc ccggtcgtca cttgacggct gacccagatg atagcatccc tccgctgggt
      781 gcgcgtttcc gtttgaaagc cgattacgat attagcggct tccctcctga aatgcaggtc
      841 atcttacagg cgttgaaaac ctacggcatg ttcctggcag ataacggttc agactggtat
      901 gtgagcggcg ccccagatga gcgctgggac aatgacatgt tgcatctatt ggatgttttg
      961 gtcggaagcg attttgaggt ggtggatgct tcaggcatga tcgtggatgt ggattcagga
     1021 gaggcaaaat agaaaaaggc tttgaaagcc tggaggaata tttaacatga gcaactcagt
     1081 acatgaaaca atacctcaga accgaaaagg acggtgggga agatttgggc ttgcaattag
     1141 tattttgagt caggtggtat tgctatgttc aattatttct ttcttttcca gcttccaatt
     1201 tatggaaggc atgttcgtgc caattctatt cgttagttta cctctggcag tgattgcaat
     1261 tttcctgatc gttatcgggc tggtcctatg tatcgtcgga atttttacac aagatgggtc
     1321 cagaaagacc gccctcattg ggttgggcgt attatcgttt accttcgtgt ttctgttgat
     1381 cgcatatcta cgtcttaatc cataatagtt ttattaatta catttatgtg aaaggtgact
     1441 ttaataatga ctattcctca accttcaacg aacgatacca aaaacaaaaa gaagtatttc
     1501 actaccctgg cttttatttt caatgcaatg ggaattctgc ttgcccttct tatcatccct
     1561 gcggtatatc tcagctacat accgtttgcc attctcttaa cagctccagc catgcttatc
     1621 attggagttt tggctatcat tctttgggca attggcttat accagggcat aaaagcaatt
     1681 cgttctgaag aaactcgtaa aatcggcgca tgggttgcga ctttactaaa tggcttcctc
     1741 ataattggcg caacatgtag tttcatagta gccacatcac tgattttctc gccgaccact
     1801 tcaattttct caacatatta tagtggtgca ggcttttcac ctgatgggaa tgtagttgcg
     1861 gttggatata aaaataaaat cctattatta gactctaaga ccggcgaaaa aataaaaaca
     1921 ttgggtggca acaaaaacgc tactgacttg atcatctttt caccagatgg gaatttacta
     1981 gctgattcct cgtatggtag gttgaccatt cgtgatatga cgacctataa gacccttaag
     2041 acctttacaa aagaaaatga tagggtattc gatttttatt tttcgccaga tgggaaactc
     2101 ataaccatta tttggtttaa taaaggtgct gaagtaatta gcacatctac ttttgaggtc
     2161 ttaaaaactg tttccttcgg gaataatttt gatactgttt ataacaaata tatttcagag
     2221 acaagccatc actttggcaa gacagataat agtgaagaaa acctatccgt ttgggaaatg
     2281 aacgatttca tcgaaaactc actaacaaaa cctatcatct ctattcctct gcctcaaagt
     2341 tctgaaaaca cttttgttta ctttgaccgc tatgacgtta ctcctgatgg gtcactcttg
     2401 gtggtgccaa ttccaacatc cattgacggc gactacaata aatctgagtt tatattgtgg
     2461 aatatcacta ccgctgaatt gatcgcaacg ctagaattca acccagtaat aggcataggc
     2521 gggtacgatt ctcacccaga ccctgaatac tatttcgctt tttcaccaga tagccaaact
     2581 cttgcgtata ttaacgagaa aaataatata accttaatcg atctaaatac atttcaaacc
     2641 agagatatcg atgttgaaga cgaatacgtg gcgcgatatt tggcatacaa ccatgatgga
     2701 agtcaaatcc ttattacaaa tgccaaatct attcaaatga taagcgttga ctctggtaat
     2761 atcgtatgga cgtatcctta attactaaat gaattccttc accccgcgcc cctctcaaca
     2821 aaagatcctc cgctataacg gcgggaggct tggcattgct gccgtgcccg gtgcaggcaa
     2881 aacgcatatt ctctcggcac tcgctgccaa gctcattcaa gaggggcggc tcggtgacga
     2941 tcaggaagtg ctcatcgtga ccctcgtcaa ctcggcggtg gacaatttcg agacgcgcat
     3001 caaacgtttc ttcgataacc cgctgcaggc gttgtataaa tatcgcgtgc gcaccttgca
     3061 tgggctggcg catgacatcg tgcgcgagaa accggcgaag gttgggctgg aggaacgctt
     3121 cagcatcatc gacgagcgtg
//
LOCUS       DAOQNJ010000299         3140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21829_length_3140_cov_9.597731, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000299 DAOQNJ010000000
VERSION     DAOQNJ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21829_length_3140_cov_9.597731"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1524
                     /locus_tag="PLG52_08955"
     CDS             115..1524
                     /locus_tag="PLG52_08955"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNB41617.1"
                     /translation="MPEREIYLLSPRALSPETIAVAFAKTSRAPESFREIAAELSDES
                     SAKFHEKWVVGYGHASVAEHAVLHIAFENVSRVAIETIEGNRLASYTEKSTRYQKWGS
                     DDFTIPPELDGHPLRDEFIATVRLLFSTYAESLDPVKNLIVERTPRRENEADEAYDRR
                     IRSQYVDRCRFLLPAAANANVGMTANARVIEMTIRKMLSHPLEEVRQIGEKLKEVSKG
                     ETPTLVKYADANPYTVETIRDLENRPSQIENRKSDWCELIEYDKDGEKKILAAALYRF
                     DEIPYANALMYVKTLKKKEREALAETLLGKLGKYDTPLRELEYSNYTFDLVMDQGAYA
                     EFKRHRMMSQTPQRLTTRLGYTTPLLITEAGFGSKYEAAMEAASQMYEKLHAFNPDVA
                     QYIVPNGFNRRVLAQFNLREAFAFCQLRTAANAHFSIRRVAQKIYEDISEVHPLLSKY
                     MKLREETWQSVEENYFSKI"
     gene            complement(1563..2549)
                     /locus_tag="PLG52_08960"
     CDS             complement(1563..2549)
                     /locus_tag="PLG52_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB41618.1"
                     /translation="MQYRHLGRTGIQVSELSIGSWVTFKNQVDVQAAVEIMAAAYDHG
                     VNFYDNAEVYAGGKSEEVMGAALKKLNWRRGSYLVSTKFYWGIEEGVNEKNTLNRKRL
                     IEGINGALKRLDMDYVDLIYCHRPDKTTPIEETVWAMHNIIEWGKALYWGTSEWAASE
                     IIEAIQIAERHHLHKPVVEQPQYNLFERKRLEGDYVRFYKEYGYGTTIWSPLASGLLS
                     GKYNKGIPADSRGALKGYEWLHGSLTNQEKLAKVAGLEPIAKELGGTVAQLAIAWCLK
                     NPYISTVITGASRVEQVHENMKSAEIAAKLTPEVMSKIDVVFGVQKKDEDDD"
     gene            2612..>3140
                     /locus_tag="PLG52_08965"
     CDS             2612..>3140
                     /locus_tag="PLG52_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB41619.1"
                     /translation="MRKTLLLLSFVIISACAPAPAQTTDVSLQAYLTATPIATSTPNV
                     LVVVETPMPTSTLQVYTVQSGDTISQIAEKFKIPQENLIAANPDVNPNSLTIGQTLFI
                     PDPSAPLAAASTPTPLPVPISQAACHPTADSGNWCFALIQNNTSGVLENVSARITLLD
                     KSGNALVYQTAYLPLD"
BASE COUNT          831 a          851 c          663 g          795 t
ORIGIN      
        1 cacctgctcg gcggcaggac aatgcgagcc ttagggtaaa atcatcgaag cgatcagcgc
       61 acaacgggct ggtcgctttt ttatgaaata gattcaacac atacaggaga caccatgccc
      121 gaacgagaga tatacctttt atccccacgc gccctcagcc cagagactat tgccgtagca
      181 tttgcaaaaa catcccgcgc ccctgaatct ttccgcgaaa tcgccgcaga acttagcgat
      241 gaatcttcag caaagttcca tgagaaatgg gtcgttggtt atggtcatgc ctctgtagcc
      301 gaacatgctg tcctgcatat cgcgtttgaa aatgtctcac gtgttgccat tgaaacaatc
      361 gaaggcaatc gccttgcttc ttatacggaa aagtccactc gctaccaaaa atggggctcc
      421 gatgacttca ccattccgcc agaattagac ggacatcctc tgcgcgatga attcatagca
      481 accgtccgcc tccttttctc gacctatgct gagtcattgg acccggtcaa gaatctcatc
      541 gttgagcgga ctccccgccg tgaaaacgaa gcggatgaag cctatgatcg ccgtatccgc
      601 tcacaatatg tggaccgttg ccgctttctg ctgcctgccg ccgccaatgc caatgtaggt
      661 atgaccgcca atgcgcgcgt catcgaaatg accattcgca aaatgctttc tcacccactc
      721 gaagaagtac gtcaaattgg cgagaagtta aaagaagtct ctaaaggtga aacgccaaca
      781 ttagtgaaat atgcggatgc aaatccatac acagtcgaaa ccattcgcga tttggaaaat
      841 cgtccatctc aaatcgaaaa tcggaaatca gattggtgcg aacttattga gtacgacaaa
      901 gatggggaaa agaaaattct cgctgcggcg ttataccgct ttgatgaaat cccctatgcc
      961 aatgctctga tgtacgtcaa aacgcttaag aaaaaagagc gtgaagcact ggctgaaacc
     1021 ttgcttggca aactcggcaa atatgatacc cctctgcgtg aattggaata ttccaactac
     1081 actttcgacc ttgtcatgga ccaaggcgca tatgccgagt tcaaacgtca ccgtatgatg
     1141 agccagaccc cacaacggct gaccacaagg ctcgggtata cgactccgct tcttataacg
     1201 gaagcaggtt tcgggtccaa gtatgaggcg gcgatggaag cagcatcaca gatgtatgaa
     1261 aagctccatg cgttcaatcc agacgttgcc caatacatcg tcccgaacgg atttaatcgc
     1321 cgggtgttgg cgcaattcaa tttacgagaa gccttcgcct tctgtcagtt gcgcacggct
     1381 gccaatgccc acttttcaat tcggcgcgtg gcacaaaaaa tttacgaaga catttccgaa
     1441 gttcatccac tgctttcaaa atatatgaaa ctgcgtgaag agacttggca aagtgtggaa
     1501 gaaaattatt tttcgaagat ctaaaaaaac tccgaggtct tgaaacctcg gagttttttt
     1561 atttaatcgt cgtcttcgtc tttcttttgc acaccaaaga ccacatcgat cttactcata
     1621 acctcgggcg tgagtttggc agcaatctct gctgacttca tattttcatg aacttgttcc
     1681 acacggcttg caccggtaat gaccgtgcta atgtagggat tcttcaagca ccatgcaatt
     1741 gccagttgcg ccacggtgcc gccaagttct ttggcaatag gctccaaacc tgcaaccttc
     1801 gccagttttt cttgattggt gaggctgcca tgcaaccatt cataaccttt gagtgcgccg
     1861 cggctatcag cagggatgcc cttgttgtat ttaccagaca gcaaaccaga agcgagcggg
     1921 ctccaaatgg tagtgccata gccatattct ttatagaagc ggacataatc gccttcgagg
     1981 cgtttgcgtt caaaaaggtt gtattgcggc tgttcaacta ccggtttatg caagtgatgg
     2041 cgttctgcaa tttgaatggc ttcaataatc tcagaagctg cccattcaga agtgccccaa
     2101 tacaaggctt tgccccattc aataatatta tgcatcgccc aaactgtttc ttcaatgggc
     2161 gtggtcttat ctgggcggtg acagtagatc aagtcaacat agtccatgtc gagacgtttg
     2221 agtgcaccgt tgataccttc aatgagacgc ttgcgattga gggtattctt ttcgttaacc
     2281 ccttcttcaa tgccccaata aaacttggtg gaaaccaaat aactaccacg gcgccagttc
     2341 aacttcttca atgcagcgcc catcacctct tccgattttc cgcctgcata cacttcggcg
     2401 ttatcgtaaa agttcacgcc gtgatcgtac gccgctgcca tgatctccac tgcggcttgc
     2461 acatccactt gattcttgaa tgtaacccaa gacccaatag ataactcact gacttgaatg
     2521 cccgttcttc caagatgacg atattgcatt gttcctccaa aagattttat ggcgaatccg
     2581 ccgtattttt cgagcacatt ataatcactg catgcgaaaa accttgctat tgctttcttt
     2641 cgtaatcata tcagcttgcg cacccgcccc tgctcaaacc acagacgtct cacttcaagc
     2701 gtaccttacg gctaccccga ttgccacatc tactcccaat gtcttggtcg tcgtcgagac
     2761 acccatgcca accagcacac ttcaagtcta taccgttcaa tcaggcgata cgatcagcca
     2821 aatcgctgag aagtttaaga ttccacaaga gaatttgatt gcggcaaatc cggatgtgaa
     2881 cccgaacagt ctcaccattg ggcagacgct cttcatccca gacccatctg cccctctcgc
     2941 cgccgcctca acccctacgc cgttacctgt tccgatttct caagccgcct gtcaccccac
     3001 cgccgactcg ggtaactggt gttttgcact cattcaaaac aatacaagcg gtgttctgga
     3061 aaatgtctcc gcccgaatca cattattaga taaaagcgga aacgcccttg tctatcaaac
     3121 cgcatatctg cccttagatg
//
LOCUS       DAOQNJ010000300         3139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_21845_length_3139_cov_6.794747, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000300 DAOQNJ010000000
VERSION     DAOQNJ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21845_length_3139_cov_6.794747"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /gene="infA"
                     /locus_tag="PLG52_08970"
     CDS             <1..191
                     /gene="infA"
                     /locus_tag="PLG52_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNB41620.1"
                     /translation="QFRVKLDNGHEVLAYLSGKMRKHYIRILLGDRVALEMSPYDMSR
                     GRITFRQRKQAAAAPVEE"
     gene            complement(252..1253)
                     /locus_tag="PLG52_08975"
     CDS             complement(252..1253)
                     /locus_tag="PLG52_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNB41621.1"
                     /translation="MTELSTTYLGLKLKNPLVASASPLSKKTDKAKKLEEAGISAIVM
                     YSLFEEQIIHESLELDHFLSRGTDSFAEALTYLPDGGMYSVSPEKYLNQVTSLKKAVN
                     IPVIGSLNGVSKGGWTSYAKRIEEAGADALELNLYFLSTDPNLTAAALETAQAELVAE
                     VKSSIKIPLAVKLSPFVTSLPNFAKRIVDAGADGLVLFNRFYQPDFDLDNLDIIHSLD
                     LSTSADLRLPLRWISILHSNVHTDYALTSGIHTAHDVLKAMMAGAKVAMMASTLLHSG
                     ETVVSSMLNDLQTWMKEREYESIQQMQGSMSQKNVKEPAAFERANYMKVLGSWRDLP"
     gene            complement(1316..>3139)
                     /locus_tag="PLG52_08980"
     CDS             complement(1316..>3139)
                     /locus_tag="PLG52_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB41622.1"
                     /translation="QAIEQIKYAIKKTYGKRGEAVVAKNFEAVDATVANIYEVKVPAK
                     VSSKTVRRLPVPNEAPDFVKNVLGQIIDSDGDNIPVSAFPVDGTFPTGTTQWEKRNLA
                     LEIPVWNEDICIQCGKCVLICPHAVIRHKVYEDASLENAPETFKHTVSKFKEFSSGYS
                     YTVQVAPEDCTGCSLCVEICPVKDKTAVGRKAINMEPQPALREAEAKNWDFFMNIPDL
                     DRKLINPSTIKNAQLLRPLFEFSGACAGCGETPYVKLVSQLFGDRAVIANATGCSSIY
                     GGNLPTTPWAKNAEGRGPAWSNSLFEDNAEFGLGMRLTIDKQKEYAEELLKSFEQTVG
                     TQLVTEILHANQADEAGIDAQRVRVEDLKSKLAKSSDSRAKDLISLAENFVKKSVWII
                     GGDGWAYDIGYGGLDHVLASGRNVNILVLDTEVYSNTGGQSSKSTPRAAVAKFSMGGK
                     GMPKKDLGLIAMSYGYVYVAKVSMGANDQQTLKALLEAESYDGPSLVIAYSPCIAHGF
                     DMARSLDQAKLAVQSGHWPIFRYDPRLAIDGKNPLQIESKEPSIPFTQYAYNETRYKM
                     LTQMNEERAEELMKEAQHDAKTRWTLYQQMASMHYESDKKE"
BASE COUNT          711 a          758 c          827 g          843 t
ORIGIN      
        1 cccagttccg tgtgaagctc gacaatggtc acgaggttct ggcgtacctt tcgggtaaga
       61 tgcgcaagca ctacattcgt attttgttgg gtgaccgtgt tgccctcgaa atgtcccctt
      121 atgatatgtc gcgcggacgc atcacgttcc gtcagcgcaa gcaagctgcc gccgctcctg
      181 tcgaagaata gtccttgcga tttgaaataa aaaaggctct ccatttggag agcctttttc
      241 gttattaagt actatggcaa atcacgccag gagccgagga ccttcatgta atttgcacgt
      301 tcaaatgccg ccggttcttt gacgttcttc tggctcatgc tgccttgcat ttgctgaatg
      361 gattcgtatt cgcgttcctt catccaggtt tgcaaatcgt ttagcatgga agagacaact
      421 gtctcgccgc tgtgcagtag ggtcgatgcc atcatggcga cctttgcgcc tgccatcatg
      481 gctttgagta cgtcatgagc ggtgtggatg ccgctggtaa gggcgtaatc tgtgtggacg
      541 ttgctgtgca aaattgagat ccagcgcagg gggaggcgta agtctgcaga tgtgctcaaa
      601 tccaagctgt ggatgatatc cagattatcg aggtcgaaat cgggttggta gaagcggttg
      661 aagagcacaa gtccgtctgc gccggcgtcc acgatgcgtt tggcgaaatt gggcagagat
      721 gtcacgaagg gactaagctt caccgccagc ggaatcttga tggaggattt tacttccgct
      781 actaattccg cttgggcggt ttcaagtgcg gcggcggtta ggtttgggtc tgtagagaga
      841 aagtataggt taagttcgag cgcatcggct ccagcttctt caatgcgttt ggcgtagctg
      901 gtccaaccgc ctttggagac gccattcaag ctaccgatta cagggatgtt gaccgctttc
      961 ttcaaactgg tcacctgatt aagatatttt tcagggctca cgctatacat accgccatca
     1021 ggtagatagg taagggcttc agcaaaagag tcggtgccgc gggaaaggaa gtggtctaac
     1081 tcaaggcttt cgtgaatgat ctgctcttca aagagagaat acatcacgat cgcggagatg
     1141 cccgcttctt ctaacttttt agctttgtcg gtcttcttag agaggggtga agccgaagcc
     1201 acaagcggat tttttagttt taagccaagg tacgtagtgg aaagttcagt catgggtaaa
     1261 gttcctttag ggagttaaga atgggcgaga aacgttctcg cccataattt aaagtttatt
     1321 cttttttgtc gctttcgtaa tgcatggatg ccatttgttg ataaagcgtc caacgggttt
     1381 tggcatcatg ctgggcttct ttcatgagtt cttcggctcg ttcctcattc atttgagtga
     1441 gcatcttata ccgggtttcg ttataagcat actgcgtgaa tgggatgctg ggttcctttg
     1501 actcgatctg aagcggattt ttaccgtcta ttgccaagcg cggatcgtag cggaagatgg
     1561 gccagtgtcc agattgtaca gcgagctttg cttggtcgag gctgcgagcc atatcgaagc
     1621 cgtgtgcaat acacgggctg tatgcaatga cgagggaagg tccatcatac gattcggctt
     1681 ccaacagagc tttcaaagtc tgctggtcgt tggcgcccat ggacaccttt gcaacataaa
     1741 cgtagccgta cgacatggcg atcagaccaa ggtctttctt gggcattccc ttgccgccca
     1801 tggagaactt ggcaactgct gcacgcggtg tggacttgga ggactgacca cctgtattcg
     1861 aatacacttc ggtatcgagc acgagaatgt tgacattgcg tccactggcg aggacatggt
     1921 ccaaaccgcc gtagccaatg tcgtatgccc aaccgtcacc accaattatc caaaccgatt
     1981 tcttgacgaa attctctgcc aaactaatca agtctttggc acggctatcg cttgatttcg
     2041 caagtttcga tttcagatct tccacgcgaa cgcgttgagc atcaataccg gcttcatcgg
     2101 cttggttggc gtgcaaaatc tcggttacca actgggtgcc taccgtctgc tcaaaggatt
     2161 tgagcaattc ttcggcatat tctttttgct tatcaatggt gaggcgcatg cctaaaccaa
     2221 actcagcgtt atcttcaaac aacgagtttg accaagcagg tccgcgacct tcggcattct
     2281 tcgcccacgg agtggtgggc aggttgccgc catagatgga agaacaaccc gtggcattcg
     2341 cgatcacggc gcggtcaccg aacaattgcg aaaccaactt gacgtacggg gtctcaccac
     2401 agccggcaca agcaccgctg aactcgaaca acgggcgcag cagttgggcg ttcttgatgg
     2461 ttgaagggtt gatcaacttg cggtcaagat cagggatgtt catgaagaaa tcccagttct
     2521 tagcttcggc ttcgcgcaaa gcgggttgcg gttccatgtt gatcgccttg cgtccaaccg
     2581 ccgttttatc cttcactggg cagatttcca cgcacaacga acagccggta caatcttctg
     2641 gcgcaacttg cactgtgtag gagtatccgc tcgaaaattc cttgaacttg gaaacagtat
     2701 gcttgaacgt ttcgggggca ttttccaaac ttgcatcttc atacactttg tgacgaataa
     2761 ctgcatgcgg gcagatcaac acacacttac cgcattgaat acaaatatct tcattccaaa
     2821 ccggaatttc caacgcgaga ttgcgctttt cccattgcgt agtgccggtg gggaaggtgc
     2881 cgtctacggg gaaggcagag acggggatgt tgtcgccgtc tgagtcgatg atctgaccga
     2941 gtacattctt gacgaagtca ggcgcttcat tcgggaccgg gagtctgcgt acggtctttg
     3001 agcttacctt cgctggaact ttgacctcat aaatatttgc cacggtcgca tccacagctt
     3061 caaagttctt tgccactacg gcttcgccgc gtttgccata agtcttcttg atggcatact
     3121 tgatttgctc aatggcttg
//
LOCUS       DAOQNJ010000301         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_22258_length_3103_cov_8.166667, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000301 DAOQNJ010000000
VERSION     DAOQNJ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22258_length_3103_cov_8.166667"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..645
                     /locus_tag="PLG52_08985"
     CDS             37..645
                     /locus_tag="PLG52_08985"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB41623.1"
                     /translation="MNEEQTWVLQAQQGNDEAFTKLVETYQTPVFNLCYRMLGEPELA
                     EDAAQETFLRAYQHLHRYDQKRPFPTWLLSIAAHYCIDRLRRRKFSMFSMDTEDEEGN
                     SFEIPDVDAPNPEGEAITGQTNERVHAMLKDLDATDRAAIIMRYWYDYSEKEIAESLN
                     LTVSAVKSRLHRARKELAGLWAEQEDDLLAEMERRHHESPAF"
     gene            626..1156
                     /locus_tag="PLG52_08990"
     CDS             626..1156
                     /locus_tag="PLG52_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNB41624.1"
                     /translation="MNHQPFETWLLDDKVLTPTEKRELDSHLRTCKNCTALAETGLAL
                     RSSRVATPAPGFAMRFQQKLAAQKLAEQRRRLWGMIVLIVSGVGLLGWFLAPVLMSIT
                     TSPVGFLLSAAGLFAFLFTSIQAFGEVLTVMLRMLPAFLPPYMWMVLFSGAAGIGLLW
                     AVSIWRLVRRPQGVFA"
     gene            1153..2190
                     /locus_tag="PLG52_08995"
     CDS             1153..2190
                     /locus_tag="PLG52_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41625.1"
                     /translation="MKKSTLTRILLLLALLIVPTASIHAQTPNNDDVVLFGQNYTLEE
                     GETLNGSLAVFGGNIDIKEGAEVNGSIAIFGGNITFAKDTTINGDVAVFGGNVSVEGE
                     IAGDMVLFGGQALLESKSKVRNIATFGGQVTQQEGSIVTGEITNNAPPEVDTPPSLPE
                     EPTVDIQVNPLWNVLGKFGQALLIAVIGMLLTLFLRPQLDRTADTITRQPLMAGGYGL
                     IVFFALPIALVIMVITLILIPVAAIVVLLIPLAWLFGIVALGQEVGERFSKAINQVWA
                     PVLSTGLGTFLLMLVIGFLELIPCFGWIPSAAVTLISVGAAVMTWFGTRNPPGYTPAA
                     AAVVEEVPPAS"
     gene            complement(2247..>3103)
                     /locus_tag="PLG52_09000"
     CDS             complement(2247..>3103)
                     /locus_tag="PLG52_09000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB41626.1"
                     /translation="LGCLILAALGSSAFHPVAAVQATLRGREFLKGRETTATSLFFTA
                     GQMGHFVGPIITGLILAYLKIPGMYILPVVSIPIGFALIYQLKDNHPHPQPVKTVISR
                     RNRAGITFILVLMAVATMQSWAQANMVNLIPKYIKDLGQGATVYGSMAGLFMGGSALG
                     NMIGGHFADRYPKRFVASLALLFSAIPIFVISRIGWSGWLYALVPLAGAGTGAVHSIM
                     VVLAQRIIPGGMALASGLILGFIFSSGALGLLYTGHLAEIYGFPYVLVLTTGMVLVAA
                     PLALLLKE"
BASE COUNT          775 a          834 c          771 g          723 t
ORIGIN      
        1 cgtaagagaa ggtagaaagc cttcccgagg agaaacgtga acgaagaaca gacctgggtt
       61 ctccaggcgc aacaaggtaa tgacgaggcg tttaccaaac tcgttgaaac ctaccaaacc
      121 cctgttttca acctgtgtta ccgcatgttg ggtgaaccag agctcgcaga agatgctgct
      181 caggaaacct tcctgcgcgc ctatcaacat ttacacaggt atgaccagaa acgccccttc
      241 ccgacttggt tgctctcgat cgcggcgcat tactgcattg accgtcttcg ccgccgcaag
      301 ttctcaatgt tctcgatgga taccgaagac gaagaaggta actcgttcga aattcccgat
      361 gtggatgcgc ccaaccctga aggggaagcc atcaccgggc agaccaatga acgagtccat
      421 gccatgctca aagacttgga tgccaccgac cgtgcggcaa tcattatgag atattggtat
      481 gactattcag aaaaggaaat tgccgagtcc ttgaatttga cggtgagtgc ggtgaaaagc
      541 cgcctgcacc gagcacgaaa ggaactggct ggcttgtggg ctgaacaaga agatgacctg
      601 ctcgccgaaa tggaaaggag acaccatgaa tcaccagcct tttgaaacct ggctgttgga
      661 cgataaagtc ctaaccccta cagagaaacg tgaactcgac tctcatctgc gcacttgcaa
      721 gaattgcacc gcactggcag agaccgggct tgccttacgc tcttctcggg tagcaacccc
      781 tgcacctggg tttgccatgc gcttccagca aaaactggcg gcgcaaaagc tggcagaaca
      841 acgccgccgt ctatggggca tgatcgttct catcgtcagc ggagttgggt tactcggatg
      901 gtttcttgct cccgttttga tgtcgatcac aacctcgccg gtgggctttt tgctctctgc
      961 tgccgggtta tttgcatttc tattcacatc catacaagca ttcggtgaag tgctcactgt
     1021 aatgctgcgc atgcttcctg cttttctccc tccctatatg tggatggttc tcttctctgg
     1081 agcagcgggt attgggttgt tatgggcagt atccatctgg cgcctcgttc gccgtcctca
     1141 aggagttttt gcatgaaaaa atcaacgcta acccgaatcc tgcttctgct ggcacttttg
     1201 atcgtgccta cagcaagcat ccacgcgcag accccgaata atgatgatgt cgttctattc
     1261 ggtcaaaact acacgctgga agagggtgaa acgctgaatg gcagccttgc tgtttttggc
     1321 ggcaatattg atattaaaga aggggcagag gtcaacgggt cgatcgccat ctttggtgga
     1381 aacattacct tcgccaaaga taccaccatc aacggtgatg tcgccgtctt tggtggaaat
     1441 gtcagcgttg aaggtgagat cgcaggcgat atggtgctct ttggcgggca agctttgctt
     1501 gagtcaaagt caaaggttcg caacatcgcc acctttggcg gacaagtcac tcaacaagag
     1561 ggctctatcg tcactggtga gatcaccaac aatgcaccgc ctgaagtgga tactccgcct
     1621 agcctgccag aagaaccgac agtagatata caggtcaacc cgttatggaa tgtgcttgga
     1681 aaattcggtc aggctttatt gatcgccgtc atcggcatgt tgctcacgct cttcttgcgc
     1741 ccgcaattag atcgcaccgc agacaccatc acgcgtcaac ccttaatggc tggcggctac
     1801 ggcttgatcg tattctttgc cctgccgatt gcattggtca ttatggtcat tacgttaatc
     1861 ttgatccccg ttgcggcaat tgtcgtcttg cttatcccct tggcatggtt attcggcatc
     1921 gtggcgttgg gtcaggaagt tggtgaacgc tttagcaaag ccatcaacca agtttgggcg
     1981 ccggttcttt ccaccggtct cggaaccttc ttgctgatgt tggtgatcgg tttcctagaa
     2041 ctcattccat gcttcggttg gattccctcg gcagctgtca ccctaatcag tgtgggtgcc
     2101 gccgtgatga cgtggttcgg aacgcgtaac ccaccgggat acacccccgc cgcggcggcg
     2161 gttgtggaag aagttccccc cgcctcttaa ttggactgta gcctgctaca aaccatccgc
     2221 cttggtcaac acccaaggcg gattttttat tccttcaata ataacgccaa cggtgccgcc
     2281 acaaggacca tacctgtcgt caacaccagt acatacggga agccataaat ctctgcgaga
     2341 tgaccggtat acagcaaccc taacgccccc gaagaaaaga taaagccgag aatcaaccct
     2401 gaagccagag ccatgccgcc aggaatgatg cgttgtgcca agaccaccat gatgctatgc
     2461 acggcgcccg tccctgcccc tgcgagtgga accaaagcat ataaccaccc agaccaaccg
     2521 attcggctaa tcacaaagat cggaatggca gagaaaagta aagccaatga cgccacaaac
     2581 cgcttggggt atcggtctgc aaagtgcccg ccaatcatat ttcctaaagc cgagccaccc
     2641 atgaagagtc ccgccatgct gccatagacc gtggcaccct gccccaaatc tttgatgtat
     2701 tttgggatga ggttcaccat attcgcctgt gcccaagatt gcatggtagc aactgccatt
     2761 aagaccagaa taaatgtgat gcctgcacga ttgcgtctag aaatgaccgt cttgactggt
     2821 tgcgggtgtg gatgattatc tttcaactgg tatatcaacg caaagccaat gggaatggat
     2881 accacaggca agatatacat gccaggaatt ttcaaatatg ccaagatcaa tccagtaata
     2941 ataggaccta caaaatgtcc catttgccca gcggtgaaaa ataatgaagt tgcggttgtc
     3001 tctcgccctt tcaagaattc acgtccgcgc aaagtggctt ggaccgcagc cacagggtga
     3061 aacgcagaag accccaacgc cgcaaggata agacaaccca agc
//
LOCUS       DAOQNJ010000302         3100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_22304_length_3100_cov_9.417406, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000302 DAOQNJ010000000
VERSION     DAOQNJ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22304_length_3100_cov_9.417406"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLG52_09005"
     CDS             complement(<1..245)
                     /locus_tag="PLG52_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB41627.1"
                     /translation="MSENFDVVVIGAGPAGYVAAIRASQLNQKVAIVDKQWMGGVCLN
                     VGCIPSKSLLKNAEVAHTLRHRAKDFGFSFDNLKLDY"
     gene            complement(362..1018)
                     /locus_tag="PLG52_09010"
     CDS             complement(362..1018)
                     /locus_tag="PLG52_09010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNB41628.1"
                     /translation="MPIQTSDIQLSVNGKQVYAYLADGKGSGILLLHAWWGLKPFFKQ
                     VCDRIAEQGFTVLAPDLRGGQIAKTIDEAKALMEKSDGEFTGDTVFTAKAHLQTLVKG
                     KLGLIGFSMGAAWSLIAAAYKPEDFGAVVLFYGNEGVDYGKITAKVLGHFCEVDEWEP
                     TEFVDNTFAEFAKAGVDATRHTYPGTAHWFVEEDRPEYDAAAAQLAWSRTYDFLKKTL
                     "
     gene            complement(1102..1578)
                     /locus_tag="PLG52_09015"
     CDS             complement(1102..1578)
                     /locus_tag="PLG52_09015"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNB41629.1"
                     /translation="MSDKQKDSPAINKYIHPPIVAMFYIIVALLLGYLVPVLAGMSQT
                     LKNIGLGITFVGFLCGVGAFLEFRKARTTLDPHGSVKTVVTGGIYRFSRNPIYLGFLF
                     MVIGFPLAYGSLWGLVISPFFMVTLSRLVIEKEEAYLEKKFKEQYTSYKSRVRRWL"
     gene            complement(1581..1907)
                     /locus_tag="PLG52_09020"
     CDS             complement(1581..1907)
                     /locus_tag="PLG52_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41630.1"
                     /translation="MKTLTGVLEAYYEQGWEGMIVFSFMPNDGNGLNDLITLRDGQLL
                     TIYDQNEATLWSGKIQFVKRGFFDKHKLKADIWSDSKQKGVSYADWMDWFWRQPPLKA
                     TLEFKD"
     gene            complement(1911..>3100)
                     /locus_tag="PLG52_09025"
     CDS             complement(1911..>3100)
                     /locus_tag="PLG52_09025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNB41631.1"
                     /translation="EVTVTKWLKQVGDSINELEPLLEVNTDKVDTEIPAPATGTLLQI
                     VAQEGVPAKVGELLAIIGKPGETVDSGQASIESAASSPTPVTQQTPAVIPQPQSINRD
                     LGFISPVVAKIAAEHGVDLSQVNGTGLHGRITKNDVLNFVESGKSKGTGQPSNISTFK
                     PSNLTPDTGTLGTLIKHTTMRKSIAQHMVESKHTSPHVLTVMEADMSRVAKHRAANKA
                     AFERDGVNLTFTAYFMMAIVAGLKAYPQTNSSWTDEGLLIHKNVNLGMAVSLGEDGLI
                     VPVIKKAEDLSLLAMARAVNDLANRARTKKLQPDDVKGGTFTLTNHGVSGSLFAFPVI
                     NQPQAGILGVGAMQKRVVVIDDAIAIRPMVYMSFVFDHRILDGASADWFLAKVKETLE
                     NWA"
BASE COUNT          790 a          772 c          721 g          817 t
ORIGIN      
        1 ctgtaatcca gcttgagatt atcaaacgag aaaccaaaat ccttggcgcg gtgacgcaag
       61 gtatgtgcca cttcagcatt tttcaaaagg gactttgaag gaatacaacc tacattcaaa
      121 caaaccccgc ccatccattg cttgtcgaca atggcaactt tctggttcaa ctgtgaagcg
      181 cggatggcag ctacatagcc ggcaggtccc gcaccgatca caacaacatc gaaattttcg
      241 gacatagaaa ctccattacg tttaagaaag tgaaattacg gcttgatttt actactaaag
      301 aaacacctcc catgccggga ggtgttggag ttttataagc aattttcaaa ttgagtcgtt
      361 cttagagagt cttcttcaaa aagtcgtatg tgcggctcca tgctaactgc gcggcagcgg
      421 cgtcgtattc ggggcgatcc tcttccacga accagtgggc agttcccggg taggtgtgac
      481 gcgtcgcatc cacgccggct ttggcgaact ctgcaaaggt gttatccaca aattcggtgg
      541 gctcccattc gtccacctca cagaaatgac ccagcacttt ggcagtgatc ttgccatagt
      601 ccacgccttc gttgccgtag aaaagcacga ccgcaccaaa gtcctccggt ttgtatgcag
      661 ccgcaatcag tgaccatgcc gcgcccatcg aaaaaccgat caacccaagt ttgcctttca
      721 ccagcgtttg caaatgtgcc ttcgccgtaa aaacggtatc accggtaaat tccccatcgc
      781 ttttttccat caaggctttg gcttcgtcaa tagttttcgc aatttgtccg ccacgtagat
      841 cgggggcgag cacagtaaag ccttgttcgg caatgcggtc acagacttgt ttgaagaatg
      901 gcttcagtcc ccaccaagca tgtaataaca aaatgccgga acctttccca tccgcaagat
      961 aggcataaac ttgttttccg ttcacactta attgaatgtc agaagtttga ataggcatag
     1021 caatctcctc caaatgatta taagtacatt tgttctaaaa tacaagcaaa agaaaaaggc
     1081 gactttcgcc gcctttggga tttacaacca acgcctcact ctggacttat agctggtgta
     1141 ttgctctttg aattttttct ccaaataggc ttcttctttc tctatgacaa gccgcgagag
     1201 tgtgaccatg aaaaatggtg agatgacaag cccccacaac gacccatatg caagtggaaa
     1261 gccgatcacc ataaaaagga agcccagata aatggggtta cgtgaaaaac gataaatgcc
     1321 acccgtcacc acggttttga cggaaccatg tgggtcgagc gtggtgcgtg cttttcgaaa
     1381 ttcaagaaac gcaccaactc cacagagaaa accaacaaag gtgataccca gcccgatatt
     1441 ttttagggtt tgtgacatgc ccgccaatac agggacgaga tatcctagta ataatgcaac
     1501 gatgatataa aacattgcca cgatgggtgg gtggatgtat ttattgatcg ccgggctgtc
     1561 tttttgttta tcgctcattt tcaatcctta aattcaagtg ttgccttcaa tggcggctga
     1621 cgccagaacc aatccatcca atcggcataa gagacgccct tttgttttga gtctgaccag
     1681 atatccgctt tgagtttgtg tttgtcaaaa aagccgcgct ttacgaactg gatttttcca
     1741 gaccagagtg tagcctcatt ctgatcgtag atggttagta gttgaccgtc tcgcaaggtg
     1801 atgaggtcat tcagtccatt tccatcattt ggcatgaaac tgaagacaat cataccttcc
     1861 cagccttgtt cgtaataggc ttcaagaact ccggtcagcg ttttcaattg ttatgcccaa
     1921 ttctccaagg tctctttgac cttcgccagg aaccaatctg ctgaagcgcc atccaagatg
     1981 cggtggtcga agacgaagga catatacacc atcggtcgga tcgcgatggc atcgtcaatc
     2041 acgacaactc gtttttgcat cgcgcctact ccaagaattc ccgcttgcgg ttgattgatg
     2101 accgggaatg cgaagagtga tccgctcaca ccatggttgg tcaaagtgaa tgtgccgcct
     2161 ttgacatcat ccggttggag tttcttggta cgcgcacgat ttgcaagatc gttgacagca
     2221 cgtgccatcg ccagcaggga caggtcttcg gctttcttga tgaccggcac gatcaaacca
     2281 tcttcaccca gtgagaccgc catacccaag ttgacattct tatgaatgag caagccttca
     2341 tccgtccacg aagaattggt ctgcggatag gctttcagcc ctgccacaat tgccatcatg
     2401 aaatacgccg tgaaggtgag gttgaccccg tcgcgctcga aagcggcttt gttcgcggca
     2461 cgatgctttg ccacccgaga catatccgct tccatcaccg tcaatacatg cggcgaggta
     2521 tgcttggact cgaccatgtg ctgcgcaatc gatttgcgca tggtggtgtg ctttataaga
     2581 gtgccaagtg ttccggtgtc aggtgtcagg tttgaaggtt tgaaggttga aatgttggaa
     2641 ggttgacctg tcccttttga ctttccactt tcgacgaaat tcaatacatc attcttcgta
     2701 atgcgtccat gcaaccccgt gccgttgacc tgagacaagt ccacgccatg ttcagcggcg
     2761 atttttgcca caacaggaga gatgaagcca agatcgcggt tgattgattg aggttgtgga
     2821 ataacagcag gagtttgttg agtaacaggt gttggcgaag aagcagcact ttctatggac
     2881 gcttgtccgc tgtccacagt ttcccctggt ttaccaatga tggctaacag ttcgcctacc
     2941 tttgcgggca caccttcctg tgcaacgatc tgcaagagag ttcccgtagc aggggcgggg
     3001 atttctgtgt caactttgtc tgtgtttact tcgagcaatg gttctaattc attaatagaa
     3061 tcaccaactt gtttgagcca tttggtgacc gtaacttcgt
//
LOCUS       DAOQNJ010000303         3095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_22378_length_3095_cov_5.266776, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000303 DAOQNJ010000000
VERSION     DAOQNJ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22378_length_3095_cov_5.266776"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLG52_09030"
     CDS             <1..863
                     /locus_tag="PLG52_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41632.1"
                     /translation="AGAEQQVTSTFLWNGIVWTGEMVLTRLSLFGVALIITMFAAFFF
                     DRFDESRVKPKRTKIKNASLETVVETPSHKVSAPHLTPLNVSAYRLNFTNILSIEIKL
                     LLKGQRWWWYLVAGGLMVAGFANSSETAYKVILPIAWVWPILLWSSIGCRETRHNVQS
                     ITFASPSPVWRQLPAQWLAGFILSLVVASGVALRLVIAGDLTGLAWLLIGAIFIPSLA
                     LACGVWSNGSKLFEILYILLWYLGPLNKLTQLDYLGVSGESQPLTFILTALALMAVAV
                     FGRIRQLRNG"
     gene            867..1640
                     /locus_tag="PLG52_09035"
     CDS             867..1640
                     /locus_tag="PLG52_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41633.1"
                     /translation="MQNTSYQLRFLPLLVLAMLVNILAQAVHETGHRLVYQVMGHEPV
                     WAFTKVVQMSDTTPSRPDEWTMKTYPDGATNWLKVSSLPNGNIEEVIAAAAGPLLGFL
                     SAILGLAMSRRSTKATAKQAWLTYTISIALVAVLYYLRAPIRTGGDEHNIAVSVGVAK
                     AIVEIPFALGYLACLILGLRELPTWKIRFTWLGTILLGSVATGLPMVLLDPIIIAGVD
                     AGNAWFQPVIGYSLPVFITIILTFIGVWVWSRRQTGTSK"
     gene            1649..2839
                     /locus_tag="PLG52_09040"
     CDS             1649..2839
                     /locus_tag="PLG52_09040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNB41634.1"
                     /translation="MKTLGIGLLTLVSALLLYAYLAYPAEYVNRLLRWGDSDVYDYQK
                     FPERLIEAPDSHFEFPFALDEDRVRTQFETDSAIDDFDSFLADNRTQAFIVIQNDAII
                     YEQYFNGASRDSIVTSFSIAKSFASTLIGLAIEDSYINSVDDPITDYLPELLERDPAF
                     ADITIRHLLMMSSGIKYEEFSFVNGDDAKTYYFPDLRRLALEDTVIAGKPGEKFLYNN
                     YHPLLLGLIIERATGTTVANYLQEKIWKPIGMEYPASWSLDEHGFEKMESGINARAID
                     FAKFGRLFLHNGDWNGTQIIPAEWAAESTQADRSVDYANYYYDDFIFADGQGYYKYMW
                     WGIQQDEQNYDFIAMGNHGQFIYISPRMNLIILRFGESYGSFHGSKGWLDIFFNFASN
                     FDIQ"
BASE COUNT          735 a          813 c          721 g          826 t
ORIGIN      
        1 gtgctggggc tgagcaacaa gtaaccagca catttctctg gaatggaatt gtgtggactg
       61 gggagatggt cctcacacgg ctttctctct ttggagtagc cttgatcatc accatgtttg
      121 ccgcattctt ttttgatcgt ttcgatgaat cgcgcgtcaa gccgaaacgc acaaaaatca
      181 aaaacgcctc tctggaaacg gtagttgaga ctccttcaca taaagtatcc gcaccccatt
      241 tgacgccgct caacgtttct gcatatcgtt tgaatttcac aaacatcttg tccatcgaga
      301 tcaaactctt actcaaagga cagcgttggt ggtggtattt ggttgcgggc ggacttatgg
      361 ttgccgggtt tgctaattca tcggaaaccg cgtacaaggt cattttgcca atcgcttggg
      421 tttggcctat cttgctctgg tcttccattg gctgccgcga aacacggcac aatgtgcagt
      481 cgattacttt cgcctcgcca tccccggtgt ggcggcaact ccctgcgcaa tggctggctg
      541 ggtttatcct gtcgcttgtt gttgcaagcg gcgtcgccct gcgtcttgtc atcgctggcg
      601 atcttaccgg gctggcctgg ctgttgatcg gcgcaatttt tattccatcg cttgccctgg
      661 cttgcggagt atggagcaac ggcagcaaac tcttcgaaat tctttatatc ctgctctggt
      721 atcttggccc gctcaacaaa ttaactcagt tggattacct cggcgtctca ggggaaagcc
      781 agccccttac attcatactg accgcattgg cgcttatggc tgttgccgtg tttggacgta
      841 tacgccaact tagaaatgga taaactatgc aaaacacaag ttatcaactc cgctttcttc
      901 cattgctcgt ccttgccatg ctggtcaaca ttttggcaca ggcagtccac gaaacgggac
      961 atcgcctggt ctaccaagtg atgggacacg aacccgtctg ggcattcacc aaggtcgtgc
     1021 aaatgtcgga tacaacgcca tcgcgtcccg atgaatggac gatgaaaaca taccccgatg
     1081 gcgcgacaaa ttggttgaag gtgagttccc ttccaaatgg aaacattgaa gaagtgattg
     1141 ccgccgccgc aggtccgctg ttgggattcc tcagcgcaat cctgggttta gcgatgtcac
     1201 gccgaagcac aaaagccaca gccaaacagg catggctcac ttatacaatc tcaatagcgc
     1261 tcgttgcagt cctttattac ctccgcgccc ccatacggac aggcggcgat gaacacaata
     1321 ttgctgtaag tgtgggagta gcaaaagcaa ttgtcgaaat tccattcgcg cttgggtatc
     1381 tcgcttgttt gattttgggt ctgcgtgaat tgcccacatg gaaaattcga ttcacgtggc
     1441 ttggcacaat tctgctcggt agtgttgcaa ctggtttgcc tatggtgttg ctcgacccca
     1501 tcatcatcgc gggcgtggac gcgggaaacg catggtttca acctgtcatc ggttattcac
     1561 taccagtttt catcaccatc atcctaacct tcattggggt ttgggtttgg tcacgcaggc
     1621 aaacaggaac atcgaaatga gaagattttt gaaaactctt ggtattggtt tgctcacgct
     1681 cgtcagcgcg ctattgcttt acgcctacct cgcctacccc gctgaatacg tcaaccgcct
     1741 cctgcgctgg ggcgattcgg atgtgtacga ttatcagaaa tttcccgaac gactcatcga
     1801 agcgcctgac tcacactttg aatttccctt cgcattggat gaagaccgcg tccggactca
     1861 atttgaaact gattcagcca tcgacgattt cgactccttc ctggcagaca accgcacgca
     1921 agccttcatc gtcatccaga atgacgccat catttacgaa caatacttca acggtgccag
     1981 ccgcgactcg attgtcacat ccttctccat tgcaaagtct ttcgcttcga cattgattgg
     2041 gcttgctatt gaagatagct acatcaacag cgtggacgac cccatcaccg actacctccc
     2101 cgaattactc gaacgtgacc ccgcctttgc ggacatcacc atccgccact tgttgatgat
     2161 gtcatcgggc atcaaatacg aagaattttc tttcgtcaac ggcgacgatg cgaagaccta
     2221 ttactttccc gatctgcgcc gacttgcttt ggaggatacc gtcatcgcag gcaagccggg
     2281 cgagaaattt ctctacaaca attaccatcc cttactgctt ggtttaatta tcgaacgcgc
     2341 cacaggcaca acagtcgcaa actacttgca ggagaaaatc tggaaaccca tcggcatgga
     2401 atatcccgcc tcatggagtt tggacgaaca cggcttcgaa aaaatggaaa gcggcatcaa
     2461 tgcccgcgcc atagactttg ccaaattcgg acgcttattc ctgcataatg gtgattggaa
     2521 tggcacacag ataatccccg cagaatgggc ggctgagtct acacaagcag atcgttctgt
     2581 tgactacgcc aattattatt acgatgactt catcttcgcc gatggacaag gttattacaa
     2641 atatatgtgg tggggcatcc agcaagatga gcaaaactat gacttcatag caatgggaaa
     2701 tcatggacag ttcatctaca tctcgcctcg catgaatctg attattctgc gtttcggcga
     2761 atcatacgga agcttccacg gctcaaaagg ctggctcgac atatttttta attttgccag
     2821 caactttgat atacaataaa gtgcgctcct tgaagtttcg ggggctgatt aagtcacttt
     2881 cagggtgtat tgttcgcctc acaaccgcaa tcggatctgc ccggcgtggc atatatcatg
     2941 tatacgggta taagtttaga ccagcagtaa cagcataaat gacacacagg cggcggagat
     3001 tttctccgcc gcttttttat tctttgtggg atgcgttttg tgcctgcatt aattcacgct
     3061 ttgcccccaa gcctgagaag atctcaatcg ctttt
//
LOCUS       DAOQNJ010000304         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_22403_length_3093_cov_8.731402, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000304 DAOQNJ010000000
VERSION     DAOQNJ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22403_length_3093_cov_8.731402"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..598
                     /locus_tag="PLG52_09045"
     CDS             146..598
                     /locus_tag="PLG52_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB41635.1"
                     /translation="MISTEILSQFSLFDGLPESLLKEIAGISNEVSVKKDDFVFREGE
                     KADKLYFLLKGSIVLRVKLTSRPESITVSFVSSPYRSFGWSGVVAPHHYTSSAEADED
                     SSLLIIPAEPFMTLLEKNPEHGFTVMKRIAEVIADRLRNSHQALLKTI"
     gene            722..2935
                     /locus_tag="PLG52_09050"
     CDS             722..2935
                     /locus_tag="PLG52_09050"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNB41636.1"
                     /translation="MKSAVVEIPIEKLMEQLPESYTVADRELVMRAYTFAEEAHREQK
                     RNSGEPYINHCIAVASILTELKVPADVIAAGLLHDTVEDTPVTLADIRREFGDVVRLL
                     VDGVTKLSHLPRVSRGGEKAENAESEEEPPVQPALLGRKEDMVPETLRKTFLAMGEDV
                     RVVLIKLADRLHNMRTLGHMPEHKRRRIAAETLDIFAPLANRLGIWQLKWELEDLSFR
                     YVQPEKYKEIVESLAERRPDRERQLEEIKSNLIKLLEKNNIKAEVSGRPKHIYSIFKK
                     MQKKDKSFGMLYDVRAVRLIVPDVPSCYAALGVIHTTWRPVPGEFDDYIAAPKDNHYQ
                     SLHTAVMYDDKRPLEIQIRTQEMHMNAEYGIAAHWRYKEGGKHDKQFEERIKDLRKMM
                     EWGGEVNDATEFVESMRSDVFRDRVYIFTPRGDAIDLPAGSTPIDFAYTVHTDIGHRC
                     RGARVNGKLVPLDTELKTGDQVEILTAKRGGPSRDWLNNSLGLVKTARAKSKIRLWFK
                     KLEDEQNLATGRTTLERELQRLGIPEVNFEKLARDLGFKIPDEMFIGIGTGDLSMSKV
                     IKQFAEDEEIKDILEANTPAAPQKPTDAVEVLGLKGMLASMAKCCNPMPGDQIVGYIT
                     RGRGATIHRLDCPNILQLQDRERLLKVGWGHVEQTFPVPVKIKAYDRQGLMSDISNLL
                     SDENININNVSVNVNRSIADLRLIIEVKDLTQLSRILTRIENLPNVMEAVRNNPG"
BASE COUNT          861 a          730 c          748 g          754 t
ORIGIN      
        1 aaaaatcttg tgacattatt tgtgactgac atattgacaa tttcctgtag aatattataa
       61 acataaaggt gctgcgcttc gttctaaact cgaaactgat cgaccccatt ttccctattt
      121 caccctgaag attggaggct attgcatgat cagcacggaa attctttccc agttcagcct
      181 gttcgatggg ctcccagaat ctctgctcaa agagatcgcg ggcatcagca atgaagtttc
      241 agtaaagaaa gatgattttg tgtttcgtga aggagaaaaa gccgataagc tttatttcct
      301 actcaagggc agcatcgtct tgcgcgtcaa attgacctct cgcccagaaa gcatcaccgt
      361 gtcttttgtc tcttcaccgt accgaagttt cgggtggtct ggtgtggttg cgccgcatca
      421 ttacacctcc agcgccgaag cagacgaaga ctcaagcctg cttattatcc ctgcagaacc
      481 cttcatgacc ttgctggaaa agaaccctga gcatggcttt acagttatga agcgtattgc
      541 cgaagtgatc gcagaccgtt tgcgaaatag ccatcaggca ttgttgaaga ccatctaaga
      601 aacgaaccgc tggtttgaaa aaaccagtgg tttttatatc aagccacagc ggagggaaag
      661 ccatcaggaa acagaggcat atcaaagtcc gagagagagg cgggcgtagt aaaattcatc
      721 catgaaatcg gcagtcgtag aaatccctat cgaaaaatta atggaacaac tccccgagtc
      781 ttatactgtt gcagaccggg aactcgtgat gcgagcttat acgtttgcag aagaagcgca
      841 tcgcgaacag aagcgcaatt cgggggagcc atatatcaat cactgcattg ccgtggcaag
      901 tattttgaca gaattaaaag tgcctgctga tgttattgca gcgggtttgt tacatgacac
      961 cgtagaagat acacctgtaa ctctcgcaga tattcgccgc gagtttgggg atgtggtgcg
     1021 tttacttgtt gatggcgtga ccaagttatc tcatttgccg cgtgtttcgc gtggtggtga
     1081 aaaagcagaa aacgccgaga gtgaagaaga accacccgtt caacctgcct tgttagggcg
     1141 caaagaagat atggtgcctg aaaccttgcg caagaccttc cttgccatgg gcgaagatgt
     1201 gcgcgtggtg ctgattaaac tggcagaccg tttgcataac atgcgtacgc tgggtcacat
     1261 gccagaacat aaacgtcgcc gcattgccgc agagaccttg gacatttttg ccccgctcgc
     1321 gaatcgactg ggtatttggc aattaaagtg ggaattggaa gacttaagtt ttcgttatgt
     1381 tcagcctgaa aaatataaag agattgtcga atcactcgcc gagcgccgcc cagaccgtga
     1441 acgacaatta gaagagatca agagtaactt aatcaagctg ctggaaaaga ataacatcaa
     1501 agccgaggtc agtggacgac ctaagcacat ctactccatt ttcaagaaga tgcagaagaa
     1561 agataaatct tttggcatgc tatatgatgt gcgggctgta cgccttatcg tgccagatgt
     1621 cccttcttgt tatgctgctt taggggttat ccacaccact tggcgccccg tgcccggtga
     1681 gtttgatgat tacattgccg caccaaaaga caatcactat caatctctac acacggctgt
     1741 gatgtatgac gacaagcgcc cgcttgagat tcagatccga acccaagaaa tgcacatgaa
     1801 cgccgagtac ggtatcgcgg ctcactggcg atataaagaa ggcggcaagc acgataagca
     1861 attcgaagaa cgcatcaaag acttgcgtaa gatgatggaa tggggcggag aagtaaacga
     1921 tgcgacagaa ttcgtcgaat cgatgcgcag tgatgtgttc cgtgatcgcg tgtacatctt
     1981 tactccgcgt ggtgatgcaa ttgacctccc ggctggttct accccgatcg attttgccta
     2041 cacagtccat accgatattg gtcatcgctg ccgtggcgct cgcgtgaatg gcaaacttgt
     2101 gccattggat acagagctga aaaccggcga tcaagtagaa attctcaccg ccaaacgcgg
     2161 aggtcccagc cgcgattggt tgaacaacag tttgggtttg gtaaaaaccg cccgcgctaa
     2221 atccaagatc cgcttgtggt ttaagaagct agaagatgag caaaatcttg ccacgggtcg
     2281 taccacgctt gaacgcgagt tacaaaggct tggcattcca gaagtcaatt ttgaaaagtt
     2341 agctcgcgat cttggcttca aaattccaga tgaaatgttt attgggattg gtacaggtga
     2401 tctttcgatg agtaaggtca tcaaacagtt tgctgaagat gaagaaatca aagatattct
     2461 tgaagccaac acgcctgccg ctccacaaaa gcccacagat gccgttgaag ttctcggctt
     2521 gaaaggcatg ttggcgtcta tggcgaaatg ctgtaacccc atgcccggtg accagatcgt
     2581 gggctacatc actcgcggac gtggcgcaac cattcaccgc cttgattgcc cgaatattct
     2641 tcaattacaa gaccgcgaac gcttgctcaa agtcggctgg gggcatgttg agcaaacctt
     2701 ccctgtacca gtcaagatca aggcatatga ccgtcagggt ctgatgagcg atatttccaa
     2761 tttgctttct gatgaaaaca tcaacatcaa caacgtctcc gtcaatgtca accgcagcat
     2821 tgccgattta cgtctcatca tcgaagtcaa agacctcaca caactgagtc gtattctgac
     2881 gcgcattgaa aacctaccga atgtcatgga agccgtacgc aataacccag gctaaccatt
     2941 ctcgcttttc tcatcaggaa ataaggtgtg aaaaagagat gggggtataa tcgcggcgct
     3001 tcgatatgct ttccgttact gaagcgcgcg agcgcatcct ttcccacttc aagatcaact
     3061 caacagaaac cgtttctctt atagatgcgg caa
//
LOCUS       DAOQNJ010000305         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_22451_length_3088_cov_7.226838, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000305 DAOQNJ010000000
VERSION     DAOQNJ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22451_length_3088_cov_7.226838"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /gene="selA"
                     /locus_tag="PLG52_09055"
     CDS             complement(<1..1174)
                     /gene="selA"
                     /locus_tag="PLG52_09055"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNB41637.1"
                     /translation="MTTLRNLPSVEQLLQTETTARLIARFGRPLTLDALRGTLDDIRA
                     RFKINPEAGLPSIDSILNQAESTLTAWTASTLHPVINATGVILHTNLGRAPLSNATLH
                     AMDLVSRGYSNLEFDLETGKRGSRLIHAEGLLKKLTSAEAALVVNNCASAVLLTLSAL
                     ANKKRVVIARSQLVEIGGGFRVPDVMKQSGAKLVEIGTTNKVRVADYTSALEESTALV
                     MRAHRSNFKIVGFTEEPDFKDIVDAAHKAGIPVVDDLGSGALYDTAKYGFSHEPTVQE
                     SMQAGADVILFSGDKLLGGPQAGIIIGKKELLDRIKKHPLARAVRADKSCLAGISATL
                     THYLKDEAEREIPIVRMMSLTLKQVKGRAEAWREELGQGNVVDGESTVGGGSLPDES"
     gene            complement(1158..>1609)
                     /locus_tag="PLG52_09060"
     CDS             complement(1158..>1609)
                     /locus_tag="PLG52_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41638.1"
                     /translation="LIQKSDASIIPTMDEKLHFKPFGLSALALALLGWGGLFLLITLT
                     LPFVWSRWGFFVLLLMALTGTALPVVFYLHQRFPTEPPADANVIIRQAMWVGVYGATL
                     AWLQLGRLVTLYIILGLAGGLIAAEYFIRIREKANRRPPMIHDDYTS"
     assembly_gap    1610..1709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1710..2497)
                     /locus_tag="PLG52_09065"
     CDS             complement(<1710..2497)
                     /locus_tag="PLG52_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB41639.1"
                     /translation="MQFIWWPVFYLFAFIPILILGYVLILRRRKPYAVRFSSLSLVRE
                     AMPKSSSWKRHFPFVLFLLAISSLIMAMTRPTSTVTLPSTNSTIILAIDVSRSMCSTD
                     IEPNRMESAKAAALQFIKAQDADTQIGIVVFAGFAAIAQPPTTDRTLLEATIQNIATA
                     RRTAIGEGILMSINAISEIDKSVVSPYANVTSVPVEEGDYVPAIIVLLTDGVSTTGTD
                     PLIAAQMAKDRGVRVYTIGFGTANNNAPMNCNPYSPQPQFEEQFG"
     gene            complement(2519..>3088)
                     /locus_tag="PLG52_09070"
     CDS             complement(2519..>3088)
                     /locus_tag="PLG52_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB41640.1"
                     /translation="NDQTAIIESIKRLTPQRGTSLANGILVSLNTIANITGDDPITTL
                     DRTGMPTPTGPIDKVDESVSIILFTDGENNMDPDPVNAALFALERGIRIHTIAIGSPE
                     GVQLTVNGFTVFTKVNEATLRGLSDLTDGIYFNAQNKEDLEAVYKTIEPGIRIAYEET
                     EVTAVFAGVSILSLLIGGILSLLWFSRVP"
BASE COUNT          769 a          733 c          808 g          678 t
ORIGIN      
        1 tggattcatc cggaagcgac ccgcccccca ccgtagactc accatccact acattgcctt
       61 gacccaattc ctcccgccac gcctcagccc gacctttgac ctgcttcagc gttagagaca
      121 tcatcctcac aatgggaatt tctcgctccg cctcgtcttt gaggtaatgt gtgagcgtag
      181 cagagatgcc tgccaaacaa gatttatctg cgcggacggc tctcgccagc ggatgtttct
      241 taattctatc gagcagctcc tttttgccga tgatgatgcc cgcctgtgga ccgccaagca
      301 gtttatcgcc ggaaaagaga atcacatccg ccccagcctg catagactct tgaacggtcg
      361 gttcgtgaga gaagccatat ttggcggtgt cgtacaaagc gcccgagcca aggtcatcta
      421 cgacgggaat gccagcttta tgggcagcat cgactatgtc tttgaaatcg ggttcttcag
      481 taaagcccac gatcttgaaa ttcgagcgat gcgcacgcat gaccaaagcg gttgattctt
      541 ccaacgcaga ggtgtaatct gccacgcgga ctttgttggt ggtgccgatc tccaccaact
      601 ttgcacccga ttgcttcatc acatcgggca cgcggaagcc gccaccgatc tccactagtt
      661 gcgagcgtgc aatgaccacg cgctttttat ttgccaaggc tgaaagcgta agcaacactg
      721 ccgaagcaca gttattaacg accaatgccg cctctgcact tgtgagtttt ttcaacaacc
      781 cttcagcatg gataagacgt gacccgcgtt tgcctgtttc aaggtcgaac tcaaggttgg
      841 agtagccgcg tgagaccaaa tccatagcat gaagggtggc gttgctcaat ggggcacgcc
      901 ctagattggt atgcagaatc actccggtgg cgttaatgac cggatggagc gtagaagccg
      961 tccatgcagt gagagtggat tcggcttgat tgaggatcga gtctatagag ggcaacccag
     1021 cttcgggatt aattttgaag cgcgcgcgga tgtcatccaa cgtgccacgt aacgcatcta
     1081 aggtcaacgg acgaccaaag cgggcaatga gtcgcgcggt cgtttcggtt tgaagaagtt
     1141 gttcgacgga gggaagatta cgaagtgtag tcatcgtgga tcatgggcgg acggcgattg
     1201 gctttctcac ggatgcgaat gaaatactcg gcggcaatca gcccacctgc tagaccgaga
     1261 atgatgtata aggtcacgag gcgacccaat tgcagccagg caagagtggc gccatagaca
     1321 cccacccaca tggcttggcg aatgatgaca ttcgcatcgg caggcggctc ggttggaaag
     1381 cgttgatgta aataaaaaac gactggaagt gccgtgccag tgagcgccat aagaaggagg
     1441 acaaaaaatc cccaacggga ccaaacgaaa ggcaaggtaa gggtgatgag aagaaacaag
     1501 ccaccccagc caagaagagc aagtgctaaa gcggataaac caaatggttt gaaatgcagt
     1561 ttttcgtcca ttgttggaat tatactcgca tcagattttt gaatgagcgn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc caaactgctc ttcaaattgt ggttgtggag
     1741 aatacggatt gcaattcatg ggagcattgt tattggcagt tccaaagcca atggtataga
     1801 cgcgaacacc gcgatctttc gccatttgcg ccgcaatgag cgggtccgtg ccggtagtgg
     1861 agactccgtc tgtgagtaag acgataatcg cgggcacata atcgccttcc tctacgggca
     1921 ccgaggtcac gttggcatac gggctgacca ctgacttgtc aatttcagag atggcattga
     1981 tggacatcag aatgccttcg ccgatggcag tgcggcgtgc tgtggctata ttttgaatgg
     2041 ttgcttcaag caaggtgcgg tctgtggtgg gaggttgagc aatggcagca aacccagcga
     2101 agacaacgat gccaatttgt gtatctgcat cttgtgcttt gataaattgc aacgccgccg
     2161 ccttggcaga ctccatgcga ttgggttcaa tatcggtgga gcacatactg cgtgagacat
     2221 cgatagcgag aatgatagtg gagtttgtgg agggcaacgt cactgtagac gttgggcgag
     2281 tcattgccat gatgaggctt gagatcgcta acaggaacaa tacgaacggg aaatgccgct
     2341 tccacgaaga gcttttgggc atcgcctcgc ggaccaaaga taggcttgaa aaccgcacgg
     2401 catagggttt gcgtcgacgc agaatcagca catacccaag aatcagaatt gggatgaacg
     2461 caaaaagata aaagacaggc caccaaataa attgcattag aacctcaatc gaccatcatt
     2521 acggcacacg gctaaaccac aataaagata agatgccgcc gatcaataaa cttagaatgc
     2581 tgacacccgc aaaaacagcg gtcacttcgg tctcttcata agcgatgcga atgcccggct
     2641 cgatggtttt atatacggct tccaaatctt ctttattctg ggcattgaaa tagatgccat
     2701 cggtcaaatc agacagtccg cgcagggtgg cttcgttcac tttggtaaaa acggtaaagc
     2761 cattgacggt caactgcaca ccctcagggc tgccaatcgc aatggtgtgg atgcgaatgc
     2821 cgcgttccaa agcgaacaac gccgcgttga ccgggtctgg gtccatgttg ttttcgccat
     2881 cggtgaagag aatgatagaa accgactcat ccactttgtc tatcggtccg gtgggagttg
     2941 gcatgccggt acggtctaac gtagtaatcg ggtcatcgcc ggtgatgttg gcaatcgtgt
     3001 tcagcgaaac gagaattcca tttgcaagag acgttccacg ttggggcgtg aggcgtttaa
     3061 tcgattcgat aattgccgtc tggtcatt
//
LOCUS       DAOQNJ010000306         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_22473_length_3086_cov_9.060376, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000306 DAOQNJ010000000
VERSION     DAOQNJ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22473_length_3086_cov_9.060376"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PLG52_09075"
     CDS             <1..921
                     /locus_tag="PLG52_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNB41641.1"
                     /translation="LKYSSLITPFTVVDVDMNTGKWDIKKVEEIPSGFDKEKYVLERI
                     HATSHDGTQVPMTIAYRNDMQKKDGTNPALMYGYGSYGATIDSYFDSNRFSLIDRGFV
                     YAIAHIRGGYDMGRDWYEQGKMENKRNTFLDFIACGEHLIHEGYVAKDKLAIQGGSAG
                     GLLVGACMTMRPDLFKAVIAKVPFMDVVTTMEDPSIPLTTQEYTQWGNPADKKFFEYM
                     LSYSPYDNLRATDYPNLLITTGLNDPRVAFWEPAKFTAKLRELKTDDNLVVFYVNYDS
                     GHAGASGRFDYIKEIALDYAFLMDRLNIKS"
     gene            complement(973..>1260)
                     /locus_tag="PLG52_09080"
     CDS             complement(973..>1260)
                     /locus_tag="PLG52_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNB41642.1"
                     /translation="RLADMVFDVVPAVLKEQGKAKNPAPNVDAISGTLQHYYGVREFD
                     FYTVLFGVGRALGVTPNYVWMRALGMPIERPKSLTTKMLEDVVAKAGQPAA"
     assembly_gap    1261..1360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1361..2050)
                     /locus_tag="PLG52_09085"
     CDS             complement(<1361..2050)
                     /locus_tag="PLG52_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNB41643.1"
                     /translation="MILHEKMSAQLPAWRERIKSLAKEHGEVVVDKVTVGQIVGGMRD
                     IKSLLTDVSFVDPAEGIRFRGMSIPEVLKALPKAKGGKMPLVGGLFYLLLIGEVPTKE
                     QALEVEAEWAKRANVPDYVFKMLKTMPKETHPMTLFSQAVLALQNESVFAAKYHSMKK
                     DQYWEAALEDSLDLTAKLPIIAAYIYRMKFFGETSKPKYNPKQDYGLNFARMMKVSDK
                     KGYADLARLYFI"
     gene            complement(2122..>3086)
                     /gene="mdh"
                     /locus_tag="PLG52_09090"
     CDS             complement(2122..>3086)
                     /gene="mdh"
                     /locus_tag="PLG52_09090"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNB41644.1"
                     /translation="LADPIGGSTMKKISIIGAGNTGATAAHWIAERELADVVLLDVVE
                     GMPQGKSLDLLEAMPIIGKDSHVLGTNNYEDTKNSDIIIITAGIARKPGMSREDLLKT
                     NAEIVGKAATETLKHSPNAIFIVLTNPLDTMAYLVMKKTGLPRERVIGQAGILDSARM
                     RAFVAMEANVSVENVQCYVLGGHGDEMVPLTRHSNIAGVPLKEYFPADKLEAIVNRTR
                     KGGGEIVNLLKTGSAYYAPSMACVQMAEAILKDKKLIVPCATLMQGEYGLNDMYFGVP
                     VMLGAGGMEKVIEYKFDADEKAMFEKSAASVRESHDALKSVVSL"
BASE COUNT          775 a          743 c          699 g          769 t
ORIGIN      
        1 ctgaaatatt cttccttgat tacaccgttt accgttgttg atgtggatat gaataccggc
       61 aaatgggata ttaagaaagt ggaagaaatc ccaagtggat ttgataaaga aaagtatgtt
      121 cttgaacgaa tccatgccac ttctcacgat ggaacccaag ttcccatgac cattgcctac
      181 cgaaacgata tgcaaaagaa agacggtacc aacccagcat tgatgtatgg atacggctcg
      241 tacggtgcga ctattgattc ttacttcgac tcgaatcgtt ttagtctaat cgaccgtggt
      301 tttgtgtatg ccattgctca catccgcggc gggtatgaca tggggcgtga ttggtacgaa
      361 caaggcaaga tggaaaacaa gcgcaatacc tttttagact ttatcgcttg cggtgaacat
      421 ctcatccacg aaggttatgt tgccaaagac aagcttgcca ttcaaggagg ttccgcaggg
      481 gggttgctcg taggtgcctg catgaccatg cgacctgatt tgttcaaagc cgtcatcgct
      541 aaagtaccct ttatggatgt ggtcactacc atggaagacc cgagcattcc gttgaccacg
      601 caggaatata ctcaatgggg caacccggca gataaaaagt ttttcgaata tatgctttca
      661 tattcaccgt acgataatct tcgcgctaca gattatccca acctgctcat tacgactggg
      721 ttgaacgatc cacgtgtggc attttgggag cctgccaagt tcacagcaaa gttacgcgag
      781 ctaaagacag acgacaatct ggtagtcttt tacgtcaact atgactcagg tcacgccggg
      841 gcaagtggac gtttcgatta catcaaagaa atcgcattag attacgcctt tttgatggat
      901 cgcttaaaca tcaaatcata attaaataaa aaaagacctt cgctaatagc gaaggtcttt
      961 tttgtttcaa atttatgcgg caggttgacc cgctttggca accacatctt caagcatctt
     1021 ggtcgtcaga gacttgggac gttcgatggg catgccgagc gcgcgcatcc acacatagtt
     1081 gggggtcacc ccgagggcgc gacctacacc aaataagacc gtgtagaaat cgaattcacg
     1141 cacaccatag taatgttgaa gcgtgccgct aatggcatct acgttcgggg caggattttt
     1201 ggctttgcct tgttccttca acacagcagg gaccacgtcg aagaccatat cggcaaggcg
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gatgaagtac aagcgggcaa
     1381 ggtcggcata gcctttctta tccgaaacct tcatcatacg agcgaagttc aaaccataat
     1441 cttgtttggg attgtatttc ggtttgctgg tttcaccaaa gaatttcatg cggtagatat
     1501 acgccgcaat gataggaagt ttcgccgtca agtcgaggct gtcttcgagg gcggcttccc
     1561 aatattgatc tttcttcatg ctgtgatact tcgccgcaaa gaccgactca ttctgcaaag
     1621 cgagcaccgc ttgcgagaag agcgtcatgg ggtgagtttc tttcggcatg gtcttcaaca
     1681 tcttgaagac ataatcaggc acgttcgcgc gtttcgccca ttctgcttcc acttcaaggg
     1741 cttgttcctt ggtaggcact tcgccgatca acagaaggta gaacaagcca cccactaaag
     1801 gcatcttgcc gccctttgcc ttgggcagcg cctttagcac ttctggaata gacataccgc
     1861 ggaagcgaat gccttcagcc gggtctacaa aagagacatc ggtgagcagg gatttgatat
     1921 cgcgcatacc gccaacgatc tgaccgacgg taactttatc aaccacaact tcgccgtgtt
     1981 ctttagcaag gctcttaatg cgttcgcgcc aggcaggcaa ttgggcagac attttttcgt
     2041 gcaaaatcat gggagtctcc tattgatttt caatacaaaa gtcgaaagcc attctgacct
     2101 tcgacttttg acctttgatg gttatagact cacaacgctc ttgagggcgt cgtgtgattc
     2161 tcgaacagaa gcagccgatt tctcgaacat tgccttttcg tcggcatcaa acttgtattc
     2221 gatcactttt tccatgccgc ctgcacccaa catgacgggc acaccgaagt acatatcgtt
     2281 caaaccatat tcaccctgca tcaacgtagc gcaagggacg atgagtttct tatccttcaa
     2341 aatggcttct gccatctgaa cacaagccat ggaaggcgca taataggcag aacccgtctt
     2401 gagcaagttc acgatctcgc cgccgccctt gcgggtgcgg ttgacaatag cttcaagttt
     2461 atcagcaggg aaatattcct tgagcggaac gcctgcaatg ttggagtgac gagtaagcgg
     2521 aaccatttcg tcgccgtgcc cacccaacac atagcattga acattttcaa cagaaacatt
     2581 ggcttccatc gcaacgaaag cacgcatacg ggcagagtcc aaaatgcccg cctgcccaat
     2641 gacacgttcg cgcggtaaac cagttttctt catgaccaag tacgccatcg tatcaagcgg
     2701 gttggtcagc acaataaaaa tagcattcgg agaatgctta agggtttcag tagcagcctt
     2761 gcccacgatc tcggcattgg tcttgagcaa gtcttcgcgg ctcatgccgg gcttgcgagc
     2821 aataccagca gtgataataa tgatgtctga attctttgtg tcttcgtaat tgttggttcc
     2881 caacacatgc gagtctttcc caataatcgg catggcttcg agcaggtcca ggctttttcc
     2941 ttgcggcatt ccctctacaa catctagcaa aacgacatcg gcgagttcac gttcggcgat
     3001 ccaatgcgca gcggtggcgc cggtattgcc cgcgccaata attgagattt tcttcatcgt
     3061 acttcctcca atgggatcgg ctaaaa
//
LOCUS       DAOQNJ010000307         3069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_22703_length_3069_cov_10.180491, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000307 DAOQNJ010000000
VERSION     DAOQNJ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22703_length_3069_cov_10.180491"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1557
                     /locus_tag="PLG52_09095"
     CDS             196..1557
                     /locus_tag="PLG52_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNB41645.1"
                     /translation="MHKKLILFLTLLTILLMPIPVYAAPHADVQADTATLDFPNTATF
                     SATLSASAEITAVTLEYGSEQLNCGEVVAKAFPDFSPSPSIDVSWTWDMRQSGSLPPG
                     TTIWWRWRYVDSAGKEYLSDTNTVIWLDNDHDWQTATGTNIRIHYYGASQSFAQSMVD
                     AGLEGLRRNKDQAGMTPDIPVDIYVYPSYDEMRDAILYEPQWTGGLAYSNFSIIIMGT
                     SGDANFDKATVIHEVTHVLVGRNAFTCIGFIPTWLNEGLAVYNEGPLDESMKAQFDIA
                     LRDDTFFPVRSLSGNFSEIPEKALLSYAQSHSVVKFMLETYGQEKMSQLLQALSDAEP
                     VDDALVQIYGVDTDGLDAAWRESLGLQPLVVAQATSQPTPTYVPTYVPIAGIPQAITA
                     TPGAIPTSSSTNNSEPSFSGPPIVLTVVMLCICLVFLTIIGVVVLGIVLRNNKGNGGK
                     KNG"
     gene            1550..>3069
                     /locus_tag="PLG52_09100"
     CDS             1550..>3069
                     /locus_tag="PLG52_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB41646.1"
                     /translation="MDKFKTMHRRFTVGGLALVLLASLFLTASAPSDPDFQIGVPLDQ
                     AIVYSGGESSNLRDYDPATTYSAGDKLIFSGLVQLDQNLNIRPDLAETWEVTGDGTIY
                     TFKIRDNAKFHNGRDVTAQDVVFSWERAASPELASDTVLTYLSDIVGVREKALGQADS
                     ISGLKVIDDKTLQVTIDAPKPYFLMKLTFPTSYIVDQDNVSAGEEWVRQPNGTGPYKL
                     KEWRSNEYIVYEANPDFYLGAPSIPYVVFKLYAGDDVRLFETGDVDIAGVGLYDVERM
                     LNPNEPLNRNLVTGVDLCTSYVTFDTTQPPFDDANVRKAFSMAFDRQRYVDVLYRGVA
                     LPAEGLYPPGLPGFDYNLQGLPFDPEQARELLKQSKYGGPEGLPEIIYTNAGIGSYIG
                     GNVAALAEMWQDYLGVKIKVENLDYNFYYQQIFSGNHGQIFDGGWCADYPDPENFADV
                     LFHTGVPQNQGGYSNPELDTLLEAARIEPDVTKRIAMYQEAERIIVNDAPVIFTTHS"
BASE COUNT          754 a          759 c          731 g          825 t
ORIGIN      
        1 ttgtgttact aaatcaaaca atctatgtcc attttacatg atgacattaa atagggctga
       61 gtttaaggtc tgatggatgt catgaattct gcgtgaattt gtcctatttc tccatgacgg
      121 ggtttaaccc cacatcagag ggaggaaagc ggctttgaaa aatggtatag ttgccaaaac
      181 gaatggagga aaaggatgca taaaaagctc atcctgtttt tgacactact cacaattttg
      241 cttatgccga ttcctgtcta tgctgcgcct catgccgacg ttcaagcgga tacagccacc
      301 ctagactttc ccaacacggc tactttttct gccaccctta gtgcttctgc agaaattaca
      361 gccgttactc tggaatatgg cagtgagcaa ttgaactgcg gggaggtggt tgccaaagcc
      421 ttcccagatt tctcccccag cccatccata gacgtctcct ggacctggga tatgcgtcag
      481 agcggatccc ttccgccagg cactaccatc tggtggcgct ggcgctatgt tgactctgct
      541 ggcaaggaat atctaagcga tacgaataca gtcatctggt tggataatga ccatgactgg
      601 caaactgcaa caggcacaaa tattcgcatc cattactatg gtgcaagcca aagtttcgcc
      661 cagtctatgg tggacgctgg tttggaaggt ctacgccgaa acaaagacca agccggtatg
      721 acgccagata tccctgtcga tatttacgtc taccccagtt acgatgaaat gcgagatgcc
      781 attctctatg aaccgcagtg gacaggcggt ttggcatact caaacttcag catcattatc
      841 atgggcactt ctggcgatgc caattttgat aaagccaccg tcatccatga agtgacccat
      901 gtgctagtag gacgcaatgc atttacttgt attgggttta ttcctacttg gttgaatgaa
      961 ggtctcgccg tgtataacga aggtccgctg gatgaaagca tgaaggcgca attcgatatt
     1021 gccctgcgtg acgatacctt tttccctgtt cgttctttga gcggaaattt ctctgaaatc
     1081 ccagaaaaag ccttgctttc atacgctcaa tctcatagtg tggtcaagtt tatgcttgag
     1141 acctatgggc aagagaagat gagccaactg cttcaagcgc ttagcgatgc cgaacctgtc
     1201 gatgatgcct tggtacaaat ttacggcgtg gataccgatg ggctcgatgc cgcttggcgt
     1261 gagtcgcttg ggcttcaacc gctggtggtg gcacaagcaa catcgcagcc cacgccgacc
     1321 tatgtgccga cctatgttcc tattgcgggc atacctcaag ccatcaccgc cacaccgggc
     1381 gcaattccaa cttcatcgtc aacaaataat tccgagcctt cttttagtgg tccgcccatt
     1441 gtgttgaccg ttgtgatgct atgtatctgt ctcgtctttt tgacgatcat cggtgtcgtg
     1501 gttttaggaa ttgtgctgcg caataataaa ggcaatggag gaaagaagaa tggataaatt
     1561 caaaaccatg catcggcgct ttactgttgg tggtttggcg cttgttttac ttgcttctct
     1621 cttccttact gccagcgcac ccagcgatcc cgattttcag attggtgttc cgttagatca
     1681 ggcgattgtt tacagtggtg gtgaatcttc aaatttgcgt gattacgacc ccgccacgac
     1741 ctatagtgcg ggcgataaac tcatcttcag cggtttggtg caactagacc aaaacttgaa
     1801 catccgtccc gaccttgccg aaacttggga agtgactggc gatggtacta tctatacctt
     1861 caagattcgc gacaatgcca aattccataa tggacgggat gttacggctc aagacgtggt
     1921 cttttcttgg gagcgcgctg ccagccctga gttggcgtct gacaccgttc tcacctactt
     1981 gagcgatatc gttggtgtac gcgagaaagc gcttggtcag gcagatagta tcagcgggct
     2041 aaaagttatc gacgacaaaa cccttcaagt cacgattgat gcgcccaagc catactttct
     2101 catgaagctc accttcccca catcctatat tgtggatcaa gacaatgtat ccgctggtga
     2161 ggaatgggtg cgtcagccca atggcacggg accttataaa ctcaaagaat ggcgctccaa
     2221 cgaatatatc gtttacgaag ccaatccaga tttctacctg ggcgcgccat cgattcctta
     2281 tgttgtcttc aaactctatg caggcgatga tgtgcgcctc tttgaaacgg gcgatgtaga
     2341 tattgcgggc gtcggtttat acgatgtaga acgtatgctg aacccaaatg agcctttgaa
     2401 ccgcaatctc gtcaccggtg tggacctttg tacaagctat gttacctttg acaccaccca
     2461 accgcctttt gacgacgcca atgttcgcaa agcctttagc atggcattcg accgtcaacg
     2521 ctatgtggat gtgttatatc gcggtgtggc attgccagca gaggggttat acccgcccgg
     2581 tttacccggc tttgattaca acctgcaagg tttacccttt gaccccgaac aagcacgtga
     2641 attgttgaag caatccaaat acggcggacc cgaaggcttg cctgagatca tttacaccaa
     2701 tgcgggtatt ggtagctata ttggtggaaa tgtagccgct ttggctgaga tgtggcaaga
     2761 ctatcttggc gtgaaaatca aggtcgagaa tttagattac aacttctact atcaacagat
     2821 tttttctggc aatcatggtc agatctttga tggtggttgg tgtgccgatt accccgaccc
     2881 tgaaaatttt gccgatgttc tcttccatac cggtgtgccg caaaatcaag gcggctattc
     2941 caacccagag ttggatacct tactcgaagc cgctcgcatc gaaccggatg tgaccaaacg
     3001 cattgccatg tatcaagaag ccgaacgcat cattgtcaac gatgcgcctg tgatcttcac
     3061 cacccattc
//
LOCUS       DAOQNJ010000308         3064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_22770_length_3064_cov_11.478232, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000308 DAOQNJ010000000
VERSION     DAOQNJ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22770_length_3064_cov_11.478232"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLG52_09105"
     CDS             <1..761
                     /locus_tag="PLG52_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41647.1"
                     /translation="LAEMPDFITDCPGSGIYHFYGGHRLWHAPEVPSRTYLPDDSPVD
                     ITQIENTLHIVQEVEAKTGLQKSFDIQMQGDCASLSITHRLTNKGLWSATTALWAITQ
                     FKPGGLGILPQTVENTGVLPNRRLTFWPYTDPSEPAVHLGKNYILINANMQGAFKVGF
                     SNPRGWQAYWWNGVLFVKRAKYEADAEYFDYGSSSESYCNHEILELETLSPITTIEPG
                     AEATHVETWEMYQGIERPQNEQDVQALVERLELE"
     gene            767..2266
                     /gene="xylB"
                     /locus_tag="PLG52_09110"
     CDS             767..2266
                     /gene="xylB"
                     /locus_tag="PLG52_09110"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNB41648.1"
                     /translation="MNYFLGIDTSTTSSKALLIDSNGVVIAVAAASHTLQTPKPLWSE
                     QNPREWWDAVAASIKSVLEKAGVSGERVVAVGLTGQMHGLILLDENGSVLRPAILWND
                     QRTQSQCDEIHQVIGKEKFIQITGNVALTGFTAPKILWVKENEPSVYARAKHVLLPKD
                     YIRFCLTGEYAMDKADGAGTVLFDLKVRDWSDEVLAALGIPREWMPRTFEGTAFTGKV
                     TEEAARLTGLKAGTPVAAGGGDQAAGAVGVGAVEPGIVGLTVGTSGVVFATTPSALIE
                     PEGRLHAFCHAVPGMWHFMGVMLSAAGSLQWYRDTLAPNVSFDELVKEAESAPAGSEG
                     LLFLPYLSGERTPYPDPLARGSFVGLTLRHTRAHMTRAVLEGVAFGLKDSFTLIQNAG
                     LGKITQVRASGGGTKGALWRQILASALEAELVTVNTSEGTAFGAALLAGVGAGAWSDV
                     ASACKSTIQITGQTLPDDAQVNSYRRSYPLYRELYPALKPSFEKISQTN"
     gene            2276..>3064
                     /locus_tag="PLG52_09115"
     CDS             2276..>3064
                     /locus_tag="PLG52_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNB41649.1"
                     /translation="MRYGHFDDINREYVIDTPETPLPWINYLGSESYFGLISNTAGGY
                     SFYKDARLRRLTRYRYNNSPLDMGGRYIYLRDNNAADSARVWSPSWMPARTPLDFYEC
                     RHGMGYTIIRSKFNGIESQTRYFVPMGENLEIWELTLTNHRDSAADISIFSAIEFNLW
                     DAMDDSSNFQRNYSIGQVEVVDDIIYHKTEYRERRDHFAYFACSEKLSGFDTQRESFL
                     GSYRGWDNPKAVEAGQATNSIAHGWQPIGSHHVKVQLKAGEQKKI"
BASE COUNT          778 a          767 c          795 g          724 t
ORIGIN      
        1 tgctcgcaga aatgccagac ttcattacag attgccccgg ttctggcatt tatcactttt
       61 atggggggca tcgcctatgg catgcccccg aagtgccaag ccgcacctac ttacccgatg
      121 actcgccagt agatatcaca cagatcgaaa acacattaca catcgttcaa gaagtagagg
      181 caaaaaccgg tttacaaaaa tcattcgata ttcaaatgca gggggattgc gcatctctca
      241 gcatcacaca tcgtttaacc aacaaaggac tttggtctgc aactaccgcc ttatgggcaa
      301 tcacgcaatt caagccgggc ggacttggca tcttgccgca aacggttgaa aatacaggtg
      361 tattgccaaa ccgcaggctc accttctggc cctataccga tccatctgaa ccggctgtgc
      421 acctcggaaa gaactatata ctgatcaacg ccaacatgca gggagcgttc aaagttggct
      481 tctcgaaccc gcgcggttgg caagcctatt ggtggaatgg agttttgttc gtaaaacgcg
      541 ccaaatatga ggcagatgcc gagtattttg actatggtag ttcaagcgaa agttattgca
      601 accatgagat ccttgagctt gagaccttat ctcccatcac aaccatagaa cccggcgcag
      661 aagctactca tgtagagaca tgggaaatgt accaaggtat tgaacgccca caaaatgaac
      721 aggatgttca agccttggta gaacgcctgg aattggagta aagcatatga actattttct
      781 cggaattgac acatccacca catctagcaa ggcattattg atcgactcaa acggcgtggt
      841 gattgcggtc gctgcggcat cgcatacgtt gcaaacaccc aagccgttat ggtcggagca
      901 aaacccgcgc gagtggtggg atgcggttgc agcgagtatc aagtctgtgt tggaaaaggc
      961 aggcgtgagc ggagaaagag tcgtcgctgt cgggctgacg ggtcaaatgc atggcttaat
     1021 ccttttggat gaaaacggga gtgtgttgcg tcctgccatc ttgtggaatg accaacgcac
     1081 gcaaagtcag tgcgatgaaa ttcatcaagt gatcggcaag gaaaaattta ttcaaatcac
     1141 cggcaatgta gcgttgaccg gttttacagc gccgaaaatt ttatgggtaa aagagaacga
     1201 gcccagtgtg tatgcaagag caaaacatgt gttgttgccc aaggattaca ttcgcttttg
     1261 tttgacaggc gaatacgcca tggacaaagc cgatggcgct ggcaccgttt tgtttgactt
     1321 gaaagtacgc gactggtccg atgaagtttt ggcggcattg ggcattccgc gcgaatggat
     1381 gccgcgcacg tttgaaggca cagcgttcac aggcaaagtg actgaagaag cagcacgtct
     1441 cactggtttg aaagcaggca cacctgtcgc cgcaggcggt ggtgaccagg cagccggcgc
     1501 tgtgggtgtg ggtgcggtag aaccgggcat tgttggtctg acggttggca ccagcggtgt
     1561 ggtctttgca acaacgcctt ccgcattgat cgagcctgaa ggtcgcttgc atgcgttctg
     1621 tcatgctgtg ccgggtatgt ggcattttat gggtgtgatg ctttctgcgg cgggaagttt
     1681 gcagtggtat cgcgacactc tcgcgcccaa tgtaagcttt gatgaattgg tgaaagaagc
     1741 cgagagtgcg cccgctggtt cggaaggttt gttgttcctt ccttatctca gcggtgaacg
     1801 cacaccttac ccagacccac tcgcacgcgg ttccttcgtt gggctaacgc ttcgtcacac
     1861 gcgcgcacat atgactcgtg ccgtgttaga aggcgtggca tttggtttga aagatagctt
     1921 caccttgatt caaaatgcag gcttgggcaa gatcacacaa gtccgcgctt cgggcggcgg
     1981 gacaaagggc gcgttgtggc gtcagattct cgcctcggca cttgaagcag aattggtcac
     2041 cgtcaacaca tccgaaggga cagcctttgg tgctgctcta ctagcaggtg ttggcgcagg
     2101 cgcatggagc gatgtcgcat cggcatgtaa atctacgatt caaattaccg ggcaaaccct
     2161 gcccgatgac gcccaagtaa acagctatcg ccggtcgtat ccgctttatc gtgagctata
     2221 cccggcgttg aaacccagct ttgaaaaaat aagccaaaca aattaaagag gttatatgcg
     2281 ttacggacat tttgatgaca taaatcgcga atatgtaatt gatactcctg agaccccact
     2341 gccctggatc aactaccttg gcagtgaatc ctatttcggt ctcatctcca acaccgctgg
     2401 cggatattcc ttttataaag atgcacgcct gcgccgcttg actcgctatc gctacaacaa
     2461 ttccccattg gatatgggcg ggcgttacat ctatttgagg gataacaacg ccgcagactc
     2521 cgctcgggtc tggtcgccaa gctggatgcc cgcgcgaacg ccgttagatt tctacgaatg
     2581 ccgtcatggc atgggctata ccatcatccg ttccaaattc aacggcatcg aatctcaaac
     2641 aagatacttc gtgcccatgg gcgagaacct cgaaatttgg gagctgactc tcaccaacca
     2701 tcgcgattcc gctgctgata tctccatctt ctctgccatc gaattcaatc tatgggacgc
     2761 catggatgac tcgagcaact tccagcgcaa ttactccatt ggtcaagtag aagtggtgga
     2821 cgatatcatt tatcacaaga ccgaatatcg tgaacgccgc gaccactttg cctattttgc
     2881 ctgctccgaa aaactgagcg gctttgatac tcaacgtgaa tctttcctcg gctcctatcg
     2941 cggatgggat aaccccaaag ccgtggaagc aggtcaagct acaaattcaa ttgcgcatgg
     3001 ctggcagccc attgggtcac accatgtcaa agtgcaatta aaggcaggcg aacagaagaa
     3061 gatc
//
LOCUS       DAOQNJ010000309         3061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_22808_length_3061_cov_11.086161, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000309 DAOQNJ010000000
VERSION     DAOQNJ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22808_length_3061_cov_11.086161"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PLG52_09120"
     CDS             complement(<1..1288)
                     /locus_tag="PLG52_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB41650.1"
                     /translation="MSYNPNVIEKKWQAKWDADKLYRSVIDNSKKKHYALTMLPYPSG
                     DLHIGHWYAMTPSDARARFMRMQGYNVLFPMGFDAFGLPAENAAIKSGIHPMVRTRDN
                     IVKMRGQLRSMGAMFDWEREAVSCEPEYYKWTEWFFIQLYKHGLAYRKMSPVDFCPNC
                     NTTLAREQVWGDDRHCERCGTPVIKKDLEQWFFKSTKYADEFLNFDGIDWPQTVKTLQ
                     TNWIDRSEGASVIFKTEVGNHDVEIFTTRPDTLWGATFMVFSPEHPLVDEITTPENKA
                     AVDEYKLQAARQSDIQRGAVGKEKTGVFTGSYAINPVNNARIPIWIADYVLMSYGTGA
                     IMAVPAHDERDFAFAKKYELSIIPVIKPEGQSEAVVGETAYIGPGTMVNSGMLDGTKV
                     NTEKGRKNPSIAKVIDWLQEKGVGKETVNYRYRDWLI"
     gene            1266..1577
                     /locus_tag="PLG52_09125"
     CDS             1266..1577
                     /locus_tag="PLG52_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41651.1"
                     /translation="MTLGLYDKSFTLGASCTFLDVTAFGVASFFARGWLTFLTVFFTE
                     GFVAAFFAAGFFAFGADFATVFLTVFAAVVFTAFFTAFWVVVFFVAFFFFAVTILTSQ
                     M"
     gene            complement(2031..2103)
                     /locus_tag="PLG52_09130"
     tRNA            complement(2031..2103)
                     /locus_tag="PLG52_09130"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2068..2070),aa:Ala,seq:ggc)
     gene            complement(2134..2206)
                     /locus_tag="PLG52_09135"
     tRNA            complement(2134..2206)
                     /locus_tag="PLG52_09135"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2171..2173),aa:Ala,seq:cgc)
     gene            complement(2265..>3061)
                     /locus_tag="PLG52_09140"
     CDS             complement(2265..>3061)
                     /locus_tag="PLG52_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB41652.1"
                     /translation="FDESDAAILSVVAAHAAIAINNARLFRARQQALEKVRAMNEVKN
                     NFLALASHELRTPLGIIIGYATFLQNDSTGEATEHAGHVLTAASQMRTLLDQMNNLTM
                     LQTDEMLLRKIKLPIQDILHFALDEIKVTASSRDLQLVLAFREDPILVQVDPEKTKLA
                     LVNILNNAIRFSPPKSELTIGAIEQGKQVMVWVQDRGEGIAVEKLQKVFEEFYQIEPP
                     NTRQHGGLGIGLTIAKGIIEAQGGSIWAESEGKGRGSTFKVLLPKA"
BASE COUNT          671 a          818 c          739 g          833 t
ORIGIN      
        1 tgatcaacca atcgcgataa cgatagttga ccgtttcttt gccaacgcct ttttcctgca
       61 gccagtcaat gaccttggcg atgctgggat ttttacgtcc cttttcagtg ttgaccttgg
      121 ttccatccaa catgcccgag ttgaccatgg tgccaggacc aatatacgca gtctcgccaa
      181 ccaccgcttc actctgacct tcaggcttga tcaccggaat aatggacagc tcatactttt
      241 tggcaaaggc gaagtcgcgt tcatcgtgcg caggcacagc catgatcgcg cccgtgccgt
      301 acgacatcaa aacataatcc gcgatccaga tcgggatgcg cgcgttgttg accgggttga
      361 tggcgtaact gccagtgaac acaccagtct tttctttgcc aaccgcaccg cgctgaatgt
      421 cggactgtcg tgccgcttgc agtttgtatt catccacagc ggctttgttt tccggcgtcg
      481 tgatctcatc caccagtgga tgttcgggtg agaagaccat gaaggtcgca ccccacaacg
      541 tgtctgggcg ggtggtgaag atctcaacat cgtggttgcc aacttcggtc ttgaagatga
      601 ccgaagcgcc ttcactgcgg tcgatccaat ttgtttgcag ggtcttcacc gtctgaggcc
      661 agtcgatgcc gtcaaagttc aagaactcat ccgcatactt ggtggacttg aagaaccatt
      721 gctccaaatc ctttttgata actggcgtgc cgcagcgttc acaatgacgg tcatcgcccc
      781 agacctgttc gcgcgccagg gtcgtgttac agttcgggca gaaatccacc ggcgacattt
      841 tgcgatatgc caaaccatgc ttgtacaact gaatgaagaa ccactccgtc cacttgtagt
      901 attcaggttc gcaagaaaca gcttcgcgtt cccaatcgaa catcgcgccc atagagcgta
      961 actgtccgcg catcttaacg atgttgtcac gagtgcgcac catagggtga atcccgctct
     1021 taatggcggc attttccgcg ggcaaaccaa aggcatcgaa gcccattggg aacaacacgt
     1081 tatagccttg catccgcata aagcgtgccc gcgcatccga cggcgtcatc gcataccagt
     1141 gaccgatgtg caagtcaccg ctggggtagg gcagcatcgt caatgcataa tgcttcttct
     1201 tgctgttatc aatcacagag cgatacaact tatccgcatc ccatttcgcc tgccattttt
     1261 tctcaatgac attggggtta tacgacaagt ctttcacctt gggcgcctct tgcactttct
     1321 tggatgtgac cgccttcggc gtcgcttctt tcttcgcccg tggctggctc accttcttga
     1381 cggttttctt cactgaaggc tttgtcgccg ctttctttgc agcaggcttc ttcgctttcg
     1441 gtgccgattt tgcaaccgtc ttcttaacgg tctttgccgc tgttgtcttc acagctttct
     1501 ttacggcttt ttgggttgtt gtcttttttg tagcgttttt cttttttgct gtaaccattc
     1561 ttacctctca aatgtaaggt tcaaactatc aggctcttca ccttgaagat ttacacaaat
     1621 gcacacggaa gccccgcaag gagcggacct tgcttccaaa aaatttgcct actgttcatg
     1681 cgtgcctcct ttcaaaatag acgatggctt aagaaacaaa aaacccttca tccacatcag
     1741 ggacgaagag ttctctccgc ggtaccaccc agattcgttt gaataaacaa acgctctttg
     1801 atcgctataa cgggcattcc cggcgcagac tacttaattc accagcgcag tttctcttgc
     1861 gagacaacag gcgagttcgg tcgtgttgtg tgctcccgtg ggcacttgaa tgctgggctt
     1921 tcacctcgct ttcccagctc gctgacggga tgacctacta ctcctgtcct aacttttaac
     1981 ttttttcatt atagcataag agcgactagt tccctaatcg ctctcgtagg tggacccagg
     2041 gggattcgaa cccccgacct tctcaatgcc attgagacgc gctcccaact gcgctatggg
     2101 cccgggtaaa tgattttttt tctctgaacg tagtggacct gaggggattc gaacccctga
     2161 cctcctcagt gcgattgagg cgcgctccca actgcgctac aggcccgtcg ttcaaaaggc
     2221 gacggctatt ctacctgagg gagaagggaa tgtcaatgaa gggattatgc ttttggcaat
     2281 agaactttga acgtcgagcc gcgccctttg ccttctgatt cagcccaaat ggagccgcct
     2341 tgtgcttcga tgattccttt ggcaatggtc agcccaattc ccaacccgcc atgttgacgg
     2401 gtgttcggtg gttcgatctg atagaactct tcaaatactt tttgtaattt ctctacggca
     2461 ataccttcgc ctcgatcttg cacccagacc atgacttgct tgccttgctc tatagcgcca
     2521 atggtcaact cgctttttgg gggtgagaag cgaatggcgt tgttgagaat attcaccaga
     2581 gcaagtttgg tcttttcggg gtctacctgc acgagaatag gatcttctcg aaacgcaagc
     2641 acaagctgaa ggtcccggct ggaggcggtg actttgatct catccaacgc aaaatgcaag
     2701 atgtcttgaa taggaagttt tatcttgcgt agaagcatct catcggtttg cagcatggtc
     2761 aggttgttca tttggtcgag cagggtgcgc atttgagaag ctgcggtgag tacatgtccg
     2821 gcatgttcgg tggcttcgcc ggttgaatca ttttgtaaaa aggtggcata gccaataatg
     2881 atgcccaaag gagtgcgcaa ctcgtgggaa gccaaagcaa gaaaattatt cttgacctcg
     2941 ttcatcgcgc ggactttttc caaggcttgt tgacgtgctc tgaagagacg agcattgtta
     3001 atggcaatgg cagcatgtgc agcgacaacg gacagaatcg ctgcatcact ttcgtcgaag
     3061 c
//
LOCUS       DAOQNJ010000310         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_22874_length_3056_cov_11.118627, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000310 DAOQNJ010000000
VERSION     DAOQNJ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22874_length_3056_cov_11.118627"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /locus_tag="PLG52_09145"
     CDS             <1..1835
                     /locus_tag="PLG52_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNB41653.1"
                     /translation="YTDFVGEMVSALSVADGAIILVDAVAGIEVGTELAWRYCDQFNL
                     PRFFVINKMDRENADFQKTYDAITEFVEAHGKRVVQVHIPIGEKQNFKGVVDVIGMKS
                     YMGDGKTIAEIPAELKEAADKAHFAMVEDAAEGEDQLMEKYLENGSLSDEEMVRGLED
                     VVYAGQFVPVFCAAGAHEIGTIALLNDIIDLIPSPAQAVKRMAQGKDGAEELKATDQG
                     PLAAYVWKTTADPFVGKMTYFRVLSGSLQADAHVWNQNKGADERMSGMHFQRGKETIA
                     AKSIHAGDLAVVSKLTVTATGDTFCDKGHPLTVEKPAYPAALYRVAVAPKTQADAAKI
                     STVLTRLSEEDMTLTWQNDPVTHETVLQGMGDQHIDVAVHRAQNKFQVGIVTHEPKVP
                     YREGITRKAQAQYRHKKQSGGAGQFGEVHLRIEPYMEGDFDFVDALVGMNLSRSYLPP
                     IEKSIRATMERGAFAGYPMSNVRVIVFDGKEHEVDSKPMAFEIAGREAFKLAVHDAGP
                     VLFEPIMTVRVTCPDANMGDVMSDLNTRRGRVQGTESERGNTTIIAHVPMSEMLKYTT
                     QIRSITGGRGYFTMEFDHYDTVPTHIAGPIIEAHKKEMEAKKEE"
     gene            complement(1897..2472)
                     /locus_tag="PLG52_09150"
     CDS             complement(1897..2472)
                     /locus_tag="PLG52_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41654.1"
                     /translation="MIKRLLPAIPFIVLIACSPDISDPGVQAAVIHSLTATVWTPTPI
                     TPSPTPEPNTAKVVELLNDAMVGADPLMETIAAKYSVIDAQVIVDTNTHQAVSLQVYV
                     DCQWIYTDGCTPENTFVILVRAFSVNNKIMGKIQEQIPPTIQTLEVLTFEQMTKTSLI
                     HIAWKDICDYTEGRINGNQLGSRMVRFAVSP"
     gene            2647..2919
                     /locus_tag="PLG52_09155"
     CDS             2647..2919
                     /locus_tag="PLG52_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNB41655.1"
                     /translation="MPIVKFPPLMKFYVENQAEFEMQAKTVAELLENILVRYPILKTH
                     LFDSQGELRRHFNIFVNGVHVRELNGMETPLKEEDKVILMASAAGG"
BASE COUNT          829 a          672 c          777 g          778 t
ORIGIN      
        1 gctacactga ttttgtaggc gagatggttt ccgccttgag tgtggcagat ggcgcaatta
       61 ttttagtaga cgccgttgcg ggcattgaag taggtaccga acttgcctgg cgctattgcg
      121 atcaattcaa tttgccgcgt ttctttgtga tcaacaaaat ggatcgtgaa aatgcggatt
      181 tccaaaaaac atacgatgcc atcactgaat ttgtggaagc gcatggaaaa cgcgttgttc
      241 aagttcacat tcccatcggc gagaagcaaa atttcaaagg tgtggtcgat gtcatcggca
      301 tgaaatcgta tatgggggat gggaagacca tcgccgaaat ccctgctgag ttaaaagaag
      361 ctgcggataa ggctcacttc gccatggtgg aagatgctgc cgaaggtgaa gaccaattga
      421 tggaaaaata tctcgagaac ggctctctct cagacgagga aatggtgcgt ggtctcgaag
      481 atgtggtcta cgccggacaa tttgtcccgg tcttttgtgc agcgggtgcg catgaaattg
      541 gcaccattgc tttgctcaat gacatcattg atttgatccc atcaccggct caggctgtga
      601 agcgtatggc acaaggcaaa gatggcgctg aagaattgaa agccacagac caaggtccat
      661 tggctgctta tgtttggaag accaccgccg acccctttgt cggaaagatg acctatttcc
      721 gcgtgctttc tggctcgcta caagcagatg cgcatgtctg gaatcaaaac aagggcgcag
      781 atgagcgcat gtctggtatg cacttccagc gcggaaaaga aacgattgct gccaagagca
      841 tccatgcggg tgatcttgcg gtcgtttcca agttgaccgt cactgcgact ggcgatactt
      901 tttgtgataa ggggcatcca ctcacagtgg aaaaacctgc ttatcctgca gccttgtatc
      961 gcgtggcagt agctcccaag acccaagccg atgcggcaaa gatttctacc gtgctcaccc
     1021 gcttgagcga agaagacatg accctcactt ggcaaaacga ccctgtgact catgagaccg
     1081 tcttgcaagg gatgggggat cagcacatcg atgtagcagt acatcgcgct caaaataaat
     1141 tccaagtggg catcgttacg catgagccga aggttccata tcgcgaaggg attacgagaa
     1201 aagcccaagc gcaatatcgc cacaagaaac aatcaggtgg tgcaggtcaa ttcggtgaag
     1261 tacatcttcg cattgaaccg tacatggaag gtgattttga ttttgtagac gccttggttg
     1321 gtatgaacct ctcgcgttct tacttaccgc ccattgaaaa gagcatccgc gctacgatgg
     1381 aacgaggcgc ctttgctggt taccccatga gcaatgtgcg tgtgatcgtt ttcgacggta
     1441 aggaacatga agtagactcg aagccaatgg catttgaaat tgctggacgt gaagccttca
     1501 agctggctgt gcatgatgct ggaccggtat tgtttgagcc aatcatgacc gtgcgtgtga
     1561 cctgtccgga tgcgaatatg ggcgatgtaa tgagtgacct caacacccgc cgtgggcgtg
     1621 tacaaggcac tgaatcagaa cgtggcaata ccaccatcat tgcgcacgtg ccgatgtctg
     1681 aaatgctcaa gtacactaca cagatccgct ctatcacagg cgggcgtggg tatttcacca
     1741 tggagttcga ccattacgat acggtgccga cccacattgc aggtccgatc atcgaagccc
     1801 ataagaaaga aatggaagcc aagaaggaag aataggcaag aaagattgaa gcatgaagag
     1861 ttccgggcaa aacccggaac tcttttttgt ttggaattag ggagagaccg cgaaacgaac
     1921 catacgagac cccaactgat ttccgttgat gcgaccttcg gtgtaatcac agatatcttt
     1981 ccacgcaatg tggatgaggc tggttttcgt catttgctca aatgtcagaa cttctagcgt
     2041 ttgaattgtg ggaggaattt gttcttggat tttccccata attttgttgt tcactgagaa
     2101 agcacgcacc aagataacaa acgtattttc gggtgtgcaa ccatctgtat agatccattg
     2161 acagtccaca tagacttgta atgagactgc ttggtgcgta ttggtatcga caataacttg
     2221 agcatcaata acactgtatt ttgctgcgat cgtttccatg agcgggtcag caccaaccat
     2281 ggcgtcattt aaaagctcta ccactttggc tgtgttcggt tcaggggtag gggatggcgt
     2341 gattggtgta ggagtccata cggttgccgt caacgaatgg ataacggcgg cttgaacacc
     2401 cgggtctgag atatctggcg agcaggcaat aaggacaata aaggggatgg ctggtaacaa
     2461 acgtttgatc atagaacctc ctttgtgcag gttttacacc cgtgcccaca tcaacgtcaa
     2521 ttccgtttgt tatacgatgc acctacgctc actcaacgat gctctcatat atataattta
     2581 tttttaaatg taaatatgca gacctgcctc ttacatagtg aaaagaactt cgtgtagaat
     2641 acaaatatgc caatcgttaa attccctcca ctcatgaagt tttatgtaga gaatcaagct
     2701 gagtttgaaa tgcaagcaaa aaccgtggct gagttattgg agaatatcct tgttcgatac
     2761 cccatactca agactcacct ttttgattct caaggggaac ttcgccggca tttcaatatt
     2821 tttgtaaatg gcgttcatgt gcgagagtta aatggaatgg aaaccccatt gaaagaagag
     2881 gataaggtta ttttgatggc atctgctgct ggtggatgac tttccaaatt atcttgacaa
     2941 aatcggtaag attcatagaa taggcgcaat ctaggtaaca gaaaaatatg aaacgtacag
     3001 tatattgctt gtgtcttggg cggggctcgt agccgccgcc tgagttaata tccgca
//
LOCUS       DAOQNJ010000311         3048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_22988_length_3048_cov_7.169395, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000311 DAOQNJ010000000
VERSION     DAOQNJ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22988_length_3048_cov_7.169395"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLG52_09160"
     CDS             complement(<1..582)
                     /locus_tag="PLG52_09160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB41656.1"
                     /translation="MKTHAEIVVIGGGIYGAQVAYHLAKNGRKDVVIVEKGEVASGES
                     SHAAGVVTQFATSQAMLRFRMYSVQLYKELGLFDTVGSLRVASSKEQLLEMERSVSRA
                     KALGLDCEVISPEESKKYMPQISDKDLYGGIYLPGDGQLDPYTVTTSMARFAKELGVE
                     IYTNTRVTGIKVSAKGEVEAVVTDKGTIRCEIIV"
     gene            complement(666..1454)
                     /locus_tag="PLG52_09165"
     CDS             complement(666..1454)
                     /locus_tag="PLG52_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNB41657.1"
                     /translation="MGKPLIPAQRREKIQEYLGIHQIARTADLMDLLEASEATVRRDL
                     EWLEQKGILERTHGGAVLNQRVIFEQEYQLRLKNYPDEKKQIGEFAATLIEEGDIVFI
                     NSGTTATQVLQHVPRNPRITVFTNNVSALVDIGDPGFSLNLTGGEFQARSNSLSGRFA
                     LDNLNQVFANKAILGVDGISLKHGLTVPTNPEAEVVRKMIERTKGQVIIVADHSKWGA
                     VSNFHVASIEAMDKLVTDTGFSKQAKKELADYSIEVFLANNMKV"
     gene            1582..2391
                     /locus_tag="PLG52_09170"
     CDS             1582..2391
                     /locus_tag="PLG52_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB41658.1"
                     /translation="MTKLVKDLMHKGVITCKPDATLGQVATLLDQHQVHALFVTDRDG
                     RIVGVISDFDLMTGEWLSSDPESLNTMRTLTASDLMTKPVDTIEAATPLTEAADLFMQ
                     KKVGRFLVLEHEKPVGTISLSDFVASLSERIKSKREKVGDVMSHAILVCRGKTPVLSA
                     AYAMTSAGWRSVLVVDLNGKVIGVVSGHDLLHLVKNGVDEKLTVRDVMHPALTIDINA
                     SLREAADMLIQNHYHRLVVVDKENPDSFPLGAISTFDIVAEMARPDSVWQK"
     gene            2491..>3048
                     /locus_tag="PLG52_09175"
     CDS             2491..>3048
                     /locus_tag="PLG52_09175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB41659.1"
                     /translation="MSEFPTQAQVVIIGGGVGGSSIAYHLTKLGWKDVVVLERHELTS
                     GSTWHSAGLVGQMRSDANLTRMMHYSTDLYRSLKAETGQDTSWREVGGIRLASSHERM
                     EETKRLVGLARSFGVPMELISPKEAQDMFPLMDITGVVGAAYTPNDGSIDPTGLTNAL
                     AIGAKNRGARFFFYTNVEKINVVNGR"
BASE COUNT          773 a          803 c          688 g          784 t
ORIGIN      
        1 gacaatgatt tcgcatcgaa tcgtgccctt atctgttaca accgcctcaa cctctccctt
       61 cgctgacact ttgattcctg tcacgcgggt gttggtataa atctccacgc ctaattcttt
      121 agcaaaacgc gccatggatg tggtgacagt gtacggatcg agttgaccgt cgccaggcag
      181 ataaataccg ccgtacagat ctttgtccga aatttgcggc atatattttt tggattcttc
      241 tggtgagata acttcacaat ccagtcccaa cgccttggca cggctcacgc tgcgttccat
      301 ttccaacaat tgttctttgc ttgaagccac gcgcaaacta ccaactgtat cgaataaacc
      361 aagttcttta tataactgca cgctatacat acggaagcgt aacattgcct gtgaggtggc
      421 aaactgcgtc accacccccg ccgcatgcga agactcaccg ctagcaacct cccctttttc
      481 gactatgacg acatccttgc gtccgttctt ggcaaggtga taagcaacct gtgccccata
      541 aattccccca ccaataacaa cgatctctgc atgagtcttc attcatcaac tccgtagttt
      601 gattgtgatg taaaacaaga aaacacgttc aaaacaaatg tcttaaaacg tgttttatat
      661 ttgtgttaga ccttcatgtt atttgcaaga aaaacttcga tcgaataatc tgccagttcc
      721 tttttcgctt gtttactaaa accagtgtcg gtaacaagtt tatccatcgc ttcgatactt
      781 gctacatgaa aattagaaac cgcaccccat ttgctatggt ctgccacaat aatgacctgt
      841 cccttcgtgc gttcaatcat cttgcggacg acttctgctt cagggttggt gggaaccgtc
      901 agcccgtgtt ttagggagat tccgtctacg ccgagaatgg ctttattagc gaagacttga
      961 ttcaaattgt ccagcgcgaa gcgaccggaa agagagttgg aacgagcctg gaattcgcct
     1021 ccggtcaaat tgagagaaaa gcccggatca ccgatatcca ccaaggcact gacgttattc
     1081 gtaaaaaccg taatgcgtgg attcctcgga acgtgctgca atacttgtgt agcagtggta
     1141 ccgctgttga taaaaacaat atcaccctct tcaataaggg tggcggcaaa ctcaccgatt
     1201 tgtttctttt cgtcgggata attcttgaga cggagttgat actcctgttc gaagatcaca
     1261 cgttgattta agaccgcgcc cccatgcgtg cgctctagga ttcctttttg ttccaaccat
     1321 tccaagtcgc ggcgaacggt tgcttcagaa gcttcaagca aatccatcag gtcggcagta
     1381 cgcgcgattt gatggatgcc aagatactct tgaatctttt ctctacgttg ggctggaatg
     1441 aggggtttac ccatctgacg gattatataa gaatctgaag gaatttgcaa gtttttgatt
     1501 ttttgcttat aatggatttg taacacagac ctgacagctt aatctcacaa aacctgtcgg
     1561 gtcttctttt ttggagactg catgacaaaa cttgtcaaag accttatgca caaaggcgtg
     1621 atcacttgca aacccgatgc aaccctgggt caagtagcta ccttgctcga tcaacatcaa
     1681 gtgcatgcct tgttcgtcac agatcgcgat ggtcggattg tgggagtcat ttcagacttt
     1741 gatttgatga caggcgaatg gctttcttca gaccctgaaa gcctcaacac catgcggaca
     1801 ctcaccgcct cagacctgat gaccaagcct gttgacacca tcgaagccgc tactcctctg
     1861 acggaagccg ccgacctttt catgcaaaaa aaggttgggc ggtttttagt tttagagcat
     1921 gaaaaaccag ttggcacgat ctcgctttca gattttgttg ccagcctttc cgagcggatc
     1981 aaatctaaac gcgagaaagt cggcgatgtg atgtcgcatg ccattcttgt ttgccgtggc
     2041 aagacaccag ttctttctgc cgcctatgcc atgacatctg ctggctggcg ctcggtgctg
     2101 gttgtggact tgaacggcaa agtcatcggt gtagtgagcg gacatgacct cttgcatctc
     2161 gtcaagaacg gcgtagatga aaaactcact gtccgcgatg tgatgcaccc cgctctcacc
     2221 atcgacatca acgccagcct gcgcgaagcc gcagatatgc tcattcaaaa tcattatcac
     2281 cgactagtcg tggtcgataa agaaaaccca gactcgtttc cacttggtgc gatctcaacg
     2341 ttcgatattg tagcagaaat ggcaagaccc gattccgttt ggcaaaagta aaaatcttaa
     2401 ccacaaaggg cacgaagtac acaaagggaa aaataaaaaa aacttcgtga ccttagtgtc
     2461 cttcgtggta aacgctctta ggagactttc atgtctgaat tcccaacaca agcccaggtc
     2521 gtcatcatcg gcggcggcgt gggtggaagc agtatcgcct atcacctcac caagctcggt
     2581 tggaaggatg tcgtcgtcct tgaacgccac gagttgacct ccggctccac ctggcactct
     2641 gcagggctag tcgggcagat gcgctctgac gcaaacctga cccgcatgat gcactacagc
     2701 accgacctat atcgcagcct caaagccgaa actgggcaag acacgtcatg gcgcgaagtc
     2761 ggcgggattc gtcttgcttc atcacacgaa cgtatggaag agaccaagcg cctcgttgga
     2821 ttagcccgct ccttcggcgt ccccatggag ttgatttctc ccaaagaagc gcaagacatg
     2881 ttcccgctca tggacatcac cggcgtggtc ggcgcagctt acactcctaa cgacggttcc
     2941 atcgacccga ccggtcttac caacgctctc gccattggcg ccaagaatcg cggcgcgcgt
     3001 ttcttcttct ataccaacgt tgaaaaaatc aacgtcgtca atgggcgc
//
LOCUS       DAOQNJ010000312         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23021_length_3045_cov_7.023077, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000312 DAOQNJ010000000
VERSION     DAOQNJ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23021_length_3045_cov_7.023077"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLG52_09180"
     CDS             complement(<1..288)
                     /locus_tag="PLG52_09180"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNB41660.1"
                     /translation="MFKYSKKILASLLALMTVLAVGSNFVPAKAAAPIVLDQENASIT
                     TGLSEEEIAGLLFMREEEKLARDVYNQLYTIWGQPIFKNIAASEQTHMDQIK"
     gene            462..1757
                     /locus_tag="PLG52_09185"
     CDS             462..1757
                     /locus_tag="PLG52_09185"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNB41661.1"
                     /translation="MRAVDIIIKKRDKGQLTAQEIDFFVRGFTNGDIPDYQASAFAMA
                     VLLNGMTNFETTDLTLSMARSGQVLDLSKIVDVAVDKHSSGGVGDKTSISVMPMVAAC
                     GLPVGKMSGRGLGFSGGTLDKLESIPGYRVNLTTDEFKAQLKEKGIVLTGQSLDLAPA
                     DGKMYALRDVTGTVPSMPLIASSIMCKKIAAGATAIVLDVKVGLGAFMETLEDGRELA
                     ELMVDIGRLAGRKTVALLSDMNQPLGSAVGNSLEMVEAIEALRGKAPYDYMEHILHVT
                     AHVLTLGGRAKDLDEGRALAEQSIKDGSALDKLRLLVKAQGGDVSYVDDTSKFERAKL
                     VEAVKAPRGGFISQVHARIVGEASVALGAGRAKKGDPIDHAVGFLILKKVGDSVQEGE
                     TLCEIHANDEKKLKEAHEAVLSAFQFSNEPVPPLPLFYE"
     gene            1823..>3045
                     /locus_tag="PLG52_09190"
     CDS             1823..>3045
                     /locus_tag="PLG52_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB41662.1"
                     /translation="MAKVSLRIYNREIERLIEQGHMDEAIAHCRHILKIFPKYLETYR
                     LLGKAYLEARRYGEAVDIFGRLLMAVPDDFVAHVGLSIIRDEENKLDDAIWHMERAFE
                     VQSSNAAIQGELQRLYGRRDGMEPPKIRMTRGALARIYVAGELYPQAIAEIRGVLAED
                     PQRVDMQVLLAHSFFKSGQRADATDLCNQLLKRYAYTFDANRIMVELIPATAGAESTQ
                     VYRMHISEMDPYSNFAKGSIFDTADVPDASVSLERFEYAGEEVQMGQDWNKSSLGFSA
                     VAPLGSGTSFADEQPDWLRAGGDETPQASSGLDAQPGAEEGIPDFLRDAGWGQASGPE
                     QPSSIFDEEPASDIIQADIPDWLKSQAPAATSLPTEPASAPPIETPDFLAGFDSGFDP
                     FAGASAPSDTPDWLK"
BASE COUNT          750 a          688 c          793 g          814 t
ORIGIN      
        1 tttgatttgg tccatatgag tctgctcgct ggctgcgata tttttgaaaa tgggttgtcc
       61 ccagattgta tataactgat tgtatacatc tcgggcaagc ttttcttctt ctcgcataaa
      121 gagtaacccg gcgatttcct cttcgctcag accagtggta atacttgcgt tttcttgatc
      181 gagaacgatt ggcgctgcag ccttggctgg aacaaaattt gaaccaacgg cgagcactgt
      241 catgagggct aaaagactag ccagaatttt tttagaatac ttgaacatgt tttatctcct
      301 ttttgagcat catttgatgt ctcttgtgat gcctgtatga tagtttttct gtgtgaaggg
      361 actgtaaaaa aatattgaag agtttaaaaa gataggaggg taatattgtc agacaaaatg
      421 ggataaaata gccgcgcaag atttcacaag gagattctta aatgcgcgcg gtagacatca
      481 ttatcaaaaa acgcgataag gggcaactca ccgctcaaga aattgatttt tttgtacgcg
      541 gttttaccaa tggggatatt ccagattatc aagcttctgc ttttgccatg gcggttttgc
      601 tcaatggcat gacgaatttt gaaaccacag acttaactct ttccatggcg cgctctgggc
      661 aggtgctcga tctttccaag atcgtggatg ttgccgtaga taaacattct tctggtggcg
      721 taggtgataa aaccagcatc tctgtcatgc caatggtggc ggcttgtggt ttgcccgttg
      781 gcaaaatgtc ggggcgcgga cttggcttca gcggcggcac actcgataaa ctcgagtcca
      841 ttcccggcta tcgcgtaaac ttaacgaccg atgaattcaa agcacaattg aaagaaaaag
      901 gcatcgtcct tactgggcaa tctctcgacc ttgcgccagc cgatggaaaa atgtatgccc
      961 ttcgtgatgt aaccggcacg gtcccttcca tgccgttgat cgcatcgtcc atcatgtgca
     1021 agaagatcgc tgcaggtgcc acggcgatcg tgttggacgt caaagtaggt ttgggcgcat
     1081 ttatggagac gctggaagac gggcgcgaac ttgctgaatt gatggtggat attggtcgct
     1141 tggcgggacg taaaaccgtt gcgcttcttt cggatatgaa tcagccgctg ggctcggcgg
     1201 tggggaattc gcttgaaatg gttgaagcga ttgaagcctt gcgcggcaaa gctccttatg
     1261 attacatgga acatattctg cacgttacag cgcatgtgct taccctcggc gggcgcgcca
     1321 aagatttgga cgaagggcgt gcattggcag agcaatctat caaagatgga agtgcgctgg
     1381 acaaattacg tctgctcgtg aaggcgcagg gcggcgacgt ttcgtatgta gatgacacct
     1441 ccaaatttga gcgggcaaaa cttgtcgagg cagttaaggc tcctcgtggc ggattcattt
     1501 ctcaggtcca cgcaagaatc gtaggcgaag cctcagtcgc attaggggct gggcgcgcca
     1561 aaaagggtga ccccatcgac catgccgttg ggtttctcat cctcaaaaaa gtgggtgatt
     1621 cggtgcagga aggcgaaact ttgtgtgaaa ttcatgccaa tgacgagaaa aagctcaaag
     1681 aggcgcatga ggcggtctta tccgcttttc aatttagtaa cgaacctgtc cctccattac
     1741 ctctgtttta cgagtaaaat atagcgtata gccttgcgat tttgaataga atcaggcata
     1801 cttaattaac aggaaaccgg taatggcaaa agtatcactg cgaatctaca accgcgagat
     1861 cgagcgtctc atcgaacagg ggcatatgga tgaggcgata gctcattgcc gccatatctt
     1921 aaagatattc cccaaatatc tcgaaaccta tcgcttgctt ggcaaagcct atcttgaggc
     1981 gcgtcgttac ggcgaagcag tggatatttt tggtcgcttg ctcatggcag tgcctgatga
     2041 ttttgtggcg catgttggtt taagcattat tcgtgatgaa gaaaataaac tcgatgatgc
     2101 catctggcac atggaacgtg cttttgaggt gcaatcctcc aatgcggcaa ttcaaggcga
     2161 attgcagcgt ttgtacggtc gccgtgatgg catggaaccg cccaagatcc gcatgacgcg
     2221 tggtgcgttg gcgcgcatct atgtggcagg tgagctgtat ccgcaagcga ttgctgaaat
     2281 ccgtggcgta cttgccgaag acccgcaacg agtggacatg caagttctgc tggctcattc
     2341 gtttttcaag agcggtcagc gtgccgatgc tactgacctt tgtaaccaac tactcaaacg
     2401 ttatgcatac accttcgatg cgaatcgtat catggtggaa ctaattcctg ctaccgctgg
     2461 cgctgaaagt acgcaagtgt atcgtatgca tatttcagaa atggatccgt attccaactt
     2521 tgcaaaaggc tccatttttg atactgctga tgtgccagac gcttctgtga gccttgaacg
     2581 ttttgaatat gctggcgaag aagttcaaat gggacaagat tggaacaaat catcccttgg
     2641 cttctctgct gttgctccgc tcggaagtgg aacttcgttt gcagatgagc aacccgattg
     2701 gcttcgtgct ggcggagatg aaacgcctca ggcttcgtct gggcttgatg ctcaacccgg
     2761 ggcagaagaa ggcattccag actttttgcg tgatgctggt tgggggcagg caagtggacc
     2821 tgagcagcct tcctctattt ttgatgaaga acctgccagt gacatcatcc aagccgatat
     2881 tccagattgg ttgaaatcac aggcgcctgc tgcaacatcg ttgccaactg aacctgcttc
     2941 agcacctccc attgaaaccc ctgattttct tgctggtttt gactcaggct tcgacccatt
     3001 tgcaggtgct tccgcaccta gcgacacccc tgattggttg aagaa
//
LOCUS       DAOQNJ010000313         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23149_length_3035_cov_9.331879, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000313 DAOQNJ010000000
VERSION     DAOQNJ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23149_length_3035_cov_9.331879"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2937)
                     /locus_tag="PLG52_09195"
     CDS             complement(1..2937)
                     /locus_tag="PLG52_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB41663.1"
                     /translation="MPKLTLHLLGGLHIHSAENPLPTFISNKVPALLAYLAVNRRAHS
                     RDKLATLLWGEMSDADSKNNLRQALTNLRKIANDDLTITRDSVELVGDVFLDSAQFEH
                     LLNHASSLDPQPASALLTDSLRLYQGDFLEGFHVREAPAFEDWILYERSRLRELALQA
                     LHALTQFHTTHGNFIEAIKFASRLLIFDPWREEAHRQLMLLYAKTGQMSAALSQYETC
                     KKILEKELGVEPSLETTDLYERIRSTRSSVRQNIPATTTGFVGRKDELISLRIKLANP
                     NCRLITLTGLGGTGKTRLAQEIARQSVDTFIHGAWMIPLAAVDAEGMLATIANAMNFS
                     FTTGNHKKQLLNFLRKKELLLVLDNFEHLLESSILVSEILQFAPEVKILVTSRERLDI
                     DGEWVVELPGLAIQSMEANQLFIQGARRAGSEIQFNENESNTITEICSLVGGLPLGIE
                     IAAAWMRSMDLRAIKAEIQKSLDFLASTRRDIPERQRSLRAVFEAAWARLSESEQKTF
                     TALSVFRGGFTREAGEQVAGNFSTLLERSLVQKSNERFELHEVTRKFAEEKLLDANTA
                     FEKHAAYYSAWAQSQARILERDSFPSMNLEFENIRAAWVWSSTQENIEHLAQIAPFTK
                     RYLDIQGRYQEGIELFSITLQKAGSPKNASDLPQNAKGHLIARLLVYTALWIADGGNP
                     EGAIQTLSSCLAYFRQAKDEHQLMVCLNGLGNANRFLHHEEQAAIYYREELEIARRIG
                     HQDEEGTALNNLALTINTLGNFEEAERLHRECLALRREMEDYPGISSSLINLGVVLFD
                     QHRYEEAKPLLDEAIKISRQYNHPRNLAASLGNLGGVLMIEKKFEEALELFLQGLEIH
                     RTTGYRFGIAIALDNVGTAHFHLGHDNESLYYLKQSIREARDIKADFIALDALVWIAN
                     LLSNRGENKMALTWLNTIKAHPHTDPETFQNMEKLRSALLNDENLEITMTTNFEEMVS
                     EALALQ"
BASE COUNT          778 a          676 c          722 g          859 t
ORIGIN      
        1 ttattgtaag gctagcgctt cactgaccat ctcttcaaag ttggttgtca tggtgatttc
       61 taggttttca tcattcaaca gggcagagcg aagcttctcc atgttttgga aagtttcggg
      121 gtctgtgtgt gggtgagctt tgatcgtatt gagccaagtg agagccattt tgttttcgcc
      181 acggtttgac aatagatttg cgatccatac taatgcgtct aatgcaataa aatcggcttt
      241 aatatcgcgg gcttcacgaa tagattgttt tagataatat aaagactcat tatcatgtcc
      301 aagatgaaaa tgcgctgtgc ctacattatc cagggcaatc gcaattccga agcggtagcc
      361 tgttgttcta tgaatctcca acccttgtaa aaacagttcc agcgcttctt caaacttctt
      421 ttcgatcatc aaaactcccc caagattgcc aagtgatgct gccaaattac gagggtggtt
      481 atactggcgc gaaattttta tggcttcatc taatagcggc ttggcttctt catatcgatg
      541 ctggtcaaat aatacaacgc ctaaattgat caagcttgat gaaatgccgg ggtaatcttc
      601 catttcacga cgcagggcta gacattccct gtgcagccgt tctgcttctt caaaattgcc
      661 gagggtgtta atggtcagag ccaggttgtt tagtgcggtt ccttcttcat cttgatgccc
      721 gattcggcgg gcgatctcca gctcttcacg ataatatata gctgcttgtt cttcgtgatg
      781 caggaaccga tttgcatttc ccaaaccatt caaacacacc atcaattgat gctcatcttt
      841 ggcttgacga aaatatgcca gacatgagct taaagtttga atggcacctt ctgggtttcc
      901 gccatctgca atccataagg cagtatagac cagaaggcgg gcaatcaagt gacctttcgc
      961 gttttgcgga aggtcacttg cgtttttagg tgaccctgct ttttgaaggg taatggaaaa
     1021 taattcgatc ccttcctgat atcgcccttg aatatcaaga taacgttttg tgaacggagc
     1081 gatctgtgcc aggtgctcaa tattttcttg tgtagaagac catacccatg cggcacgaat
     1141 attctcaaat tccaggttca tggatgggaa cgaatcgcgt tccaatatac gagcttggct
     1201 ttgagcccaa gctgaataat aagctgcatg tttttcaaag gcggtgtttg catccagcag
     1261 tttttcttcg gcaaactttc tggtcacttc atgcaattca aaacgctcgt tgcttttttg
     1321 caccaaagaa cgttcaagaa gcgttgagaa gtttcccgct acttgttcac ctgcttcacg
     1381 tgtaaagcca ccgcggaaaa cagacaacgc tgtaaacgtc ttttgttctg attcacttaa
     1441 tcttgcccat gccgcctcaa agacggctcg caaactgcgc tgcctttctg gaatatctcg
     1501 gcgggttgaa gcaagaaaat ctaaactttt ttggatctct gcttttattg cacggagatc
     1561 catcgaacgc atccacgctg cagcgatttc aatgccaagc ggaagcccgc ctaccagtga
     1621 acagatctct gtgatggtgt ttgactcatt ttcattgaac tggatttcag agcctgcgcg
     1681 cctcgctcct tggataaaaa gctggttcgc ctccatcgac tggattgcca gcccgggcag
     1741 ttccacgacc cattcgccat ctatatccaa ccgctcccgt gatgtgacaa gaattttcac
     1801 ttcgggggca aactgcaaaa tctctgaaac cagtatggac gattccagca aatgttcaaa
     1861 gttgtccagg acaagcagca gctctttttt gcgcaaaaaa ttaagcaatt gctttttgtg
     1921 attaccggtt gtaaatgaaa agttcattgc attggcaata gtggcgagca tgccttcagc
     1981 atccacagct gcaagcggaa tcatccaggc accatggatg aatgtatcca cactttggcg
     2041 ggctatttct tgtgcaaggc gtgtcttccc tgtgccgcct aatccagtta atgtaatgag
     2101 acggcagttg gggttggcaa gttttatgcg taaagagata agttcatctt tacgccctac
     2161 gaaaccagtt gtcgtagcgg gaatattctg ccttacagag ctccttgttg agcgaatacg
     2221 ctcatataag tccgttgttt ctaaggatgg ttctacgcct agttcttttt ccaggatctt
     2281 tttgcaggtt tcatattgag agagcgctgc actcatctgt ccagtttttg cataaagaag
     2341 catcagttga cgatgggctt cttcccgcca ggggtcaaag attaatagac gggatgcaaa
     2401 cttaatggct tcaataaaat ttccatgggt tgtgtggaat tgggtcaggg catgaagcgc
     2461 ttgtaaggca agctctcgta agcgggatcg ttcataaagg atccaatctt cgaaagcagg
     2521 cgcttcgcgt acatgaaagc cttcaagaaa atcgccctga tataggcgta gggaatctgt
     2581 cagcaaagca gaagcaggtt gagggtccaa agaagaggcg tgatttaaga gatgctcaaa
     2641 ttgcgctgaa tccagaaaga catccccgac tagttcaacg ctgtcacgag tgatggttag
     2701 atcgtcgttt gcaatcttgc ggagattggt gagggcttgg cgcaggttgt ttttcgaatc
     2761 tgcgtcagac atttcgcccc acaaaagcgt ggcaagctta tcgcggctat gagcacggcg
     2821 attgacggca aggtatgcca gcaaggcagg aaccttgttg gaaatgaaag tagggagggg
     2881 attttcggcg ctgtgaatgt gcagcccgcc gaggaggtgg agggtgagtt tgggcacatt
     2941 gctccaataa atgatttcta aatgatgatg ttatacacta atgcaagaat atcacaaaag
     3001 gaaatggagt tccatgccag gtctgaaacg atcac
//
LOCUS       DAOQNJ010000314         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23150_length_3035_cov_9.121812, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000314 DAOQNJ010000000
VERSION     DAOQNJ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23150_length_3035_cov_9.121812"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PLG52_09200"
     CDS             <1..1162
                     /locus_tag="PLG52_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41664.1"
                     /translation="LHAPASLLTLGLVAISFILLVWVLIRSTQAEATDKSFAISLLTI
                     GFFGILLGRLPSLAANLPLTLQSSYDRFMISIMLGSVLFVLGLLELFIKDHRTRVFVL
                     SALIALGIGQQFFNANIFRRDWQKQGEIYWQMAWRMPALAENTVLLTHQMPIDYETDL
                     SFTAPINWMYAPNYTRANLPYMLLYTEKRLGGSTLPSLDKSTSITYPYRTVQFDSTLS
                     NAIVIYMPQNGCLRVMDPSRGHAEIYDNIADEFTQAIAISDPSRILVKSTPPAEPMFF
                     PEPAKDWCYYFAMAELYNQWNEYQVSAEFADEALAAKFQPEDPREWIVFIEAYAMNGE
                     LDKAVEASNSALKMDKKTLKAVCQTWEQVQAKGPEGSAEGFESARLSLGCNP"
     gene            1230..1994
                     /locus_tag="PLG52_09205"
     CDS             1230..1994
                     /locus_tag="PLG52_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB41665.1"
                     /translation="MSNKRRSVLALSIFMITAVMLSACTQSLSSAPAETPTLLPTGLF
                     VSPIASVENPMEMIEQFAKQTEAAQTSAAGGGTPGTPGTPQAVSTITTGTPGTPAPAV
                     ASVTPSAPTVSVSTPTNANAAAPTAAVPTSSSSSAATAPSLSGNTYVLQDGEWPYCIA
                     RRYNVDPDAMLQASGLTSPDIYYAGLKLVLPQNTTFPGARVLIAHPASYTVASGDETV
                     YSIACKYGDVDPNALASANGISVSSKLTVGQVLSIP"
     gene            2068..2598
                     /locus_tag="PLG52_09210"
     CDS             2068..2598
                     /locus_tag="PLG52_09210"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB41666.1"
                     /translation="MPSFELIRSEPLMQGRAFKIRRDYLKTPDGHETKYDIVEHGGSV
                     VIVPFDGDGNLLFVRQYRHAAGMDMLELPAGTRDGDEPFEECAAREIREETGMEAGKL
                     IHIGSFYLAPGYSTEYMGVFLATDLKHNPLEADEDEFLSVEKIPVKDALKMAEKGEMP
                     DAKSLAALLMARAYLE"
     gene            2803..>3035
                     /locus_tag="PLG52_09215"
     CDS             2803..>3035
                     /locus_tag="PLG52_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41667.1"
                     /translation="MNKNIIMIDGNEATASVAHRLNEVIAIYPITPSSAMGEFSDEWS
                     AKGKKNIWGTVPLVVEMQSEGGAAGTVHGALQTG"
BASE COUNT          783 a          837 c          671 g          744 t
ORIGIN      
        1 cctacacgcg cccgcctctc ttctgacctt gggcttggtc gccatctcat tcattttgct
       61 cgtctgggtc ttaatccgct ccacccaagc cgaagcgaca gataaatcct ttgccatctc
      121 cttactaaca attggcttct tcggtattct cttagggcgc cttccctctc ttgcagccaa
      181 tcttccccta actctgcaat cctcctacga ccgttttatg atctccatca tgctcggttc
      241 ggtcttattc gtgttaggtc tgctagagtt attcatcaaa gaccatcgca cacgtgtctt
      301 tgtcctttct gccctcatcg ccctcggtat tgggcagcaa ttcttcaacg ccaacatctt
      361 ccgcagagat tggcaaaagc aaggcgaaat ctactggcaa atggcatggc gcatgcccgc
      421 attggcagag aataccgttc tcctcaccca ccaaatgccc atcgattacg aaaccgacct
      481 ctccttcacc gcgcccatca actggatgta tgcgccgaac tatacccgtg ccaatcttcc
      541 ttatatgttg ctttacacag aaaagcgttt gggcggttct accttaccgt cattagataa
      601 atctacatcc atcacctatc catatcgaac tgtacaattt gatagcacac tctcgaatgc
      661 catagtgatt tatatgccgc agaatggctg tctacgcgtc atggacccaa gccgtggtca
      721 tgccgaaatt tacgacaaca tcgccgatga attcacccaa gccattgcaa tctcagaccc
      781 atcgcgcatc ttggtcaagt ccacgccgcc agccgagccg atgttcttcc ctgagccagc
      841 caaagactgg tgctattact tcgccatggc cgaactttac aatcaatgga acgaatacca
      901 agtctctgcc gagtttgcag atgaagcgct ggcagccaag ttccagcccg aagacccacg
      961 cgagtggatc gtgttcattg aagcgtatgc catgaacggc gaattggata aagcagttga
     1021 agcctccaac tctgctctta agatggataa gaaaacgctc aaagcggtct gccaaacctg
     1081 ggagcaggtg caggcaaagg gtccagaagg aagcgcggag gggtttgaat ccgctcgatt
     1141 gtcacttggt tgcaatccat agcgctggct tgacgcttta aacttcatgt gctaccatct
     1201 ttttttagta aatagaacga ggagaatcta tgtctaataa acgccgcagt gtgctggcat
     1261 tgtcgatctt catgatcaca gctgttatgc tctccgcctg cacacaatca ctttcgtctg
     1321 ctcccgcaga aacaccaact ctcttaccaa cagggttgtt tgtttcgccg attgcttctg
     1381 ttgaaaaccc aatggaaatg atcgagcagt tcgccaagca gaccgaagct gctcaaacat
     1441 ccgccgctgg cggtggcacc ccgggcactc ccggaacccc acaagcagtt tctacaatca
     1501 ccacaggcac ccccggaaca cccgcaccag ccgtcgcttc tgttacccct tcggctccta
     1561 cggtgagtgt ttccaccccc accaatgcaa atgcagcggc tcctactgcc gcagtgccaa
     1621 cttcttcatc tagctctgct gcaacagcac ccagcctttc tggcaacacc tatgttctgc
     1681 aagatggtga atggccttac tgtattgccc gtcgttacaa cgtggaccct gacgccatgc
     1741 ttcaggcaag tggtcttacc agcccagata tttactatgc tggtttgaag ttggtccttc
     1801 cgcagaacac caccttcccc ggtgctcgcg ttttgatcgc gcacccggct tcgtacacag
     1861 tcgcttcggg tgatgaaaca gtgtacagca tcgcttgtaa atatggtgat gtagacccga
     1921 atgcactcgc gagtgcgaat ggaatttctg tctcttccaa gttgacggtt gggcaggttc
     1981 tgagcattcc gtaatttcaa aaagacaaaa gcccaggtca acagcctggg ctttttttat
     2041 agaaactatt cttgaaagga aatcactatg ccatcatttg aactcattcg ctctgaacct
     2101 ctcatgcagg gacgtgcctt caagattcgc cgtgattatt tgaaaacacc agatggtcac
     2161 gaaaccaaat acgatattgt agaacatggc ggctcggtgg tgatcgtgcc atttgacggg
     2221 gatgggaacc tgctcttcgt gcgacaatac cgtcacgcgg ctggtatgga tatgcttgaa
     2281 ctgcccgctg gtacgcgcga tggtgatgaa ccatttgaag aatgtgccgc acgtgagatc
     2341 cgcgaagaga caggcatgga agctggcaag ttgattcaca tcggttcgtt ctacctcgca
     2401 ccgggctact ccactgaata catgggcgtc ttcttagcaa cagatttgaa acacaatcca
     2461 ctagaagccg atgaagatga attcctaagc gtagaaaaaa tccccgtcaa agatgcattg
     2521 aaaatggcag agaaaggcga aatgccagat gcaaaatcat tggcagcatt gctaatggca
     2581 agagcgtatc ttgaataaac cgtttgaaaa ataacaatcg ctcaaaaaac gtcaaaacac
     2641 ctcgcaaacg tcgaggtgtt ttatttccac caatcctatc caatatatga catcaggcag
     2701 ggggatttaa gtcatatata actaacgtaa ttcatcccgc aggaataaac ttattcattg
     2761 gaaaatacga ggcattcgtg ccttttttac aggagcaaaa gcatgaataa gaatatcatt
     2821 atgatcgacg gaaacgaagc cacagccagt gtagcccacc gtctcaatga agtgatcgcc
     2881 atttacccga tcaccccatc ctctgcgatg ggcgaattct cagacgaatg gtcagctaag
     2941 ggcaaaaaga acatttgggg aaccgttcca ctcgtggttg aaatgcaatc tgaaggcggc
     3001 gcagccggaa ccgtacacgg cgccctccaa accgg
//
LOCUS       DAOQNJ010000315         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23256_length_3029_cov_12.647949, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000315 DAOQNJ010000000
VERSION     DAOQNJ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23256_length_3029_cov_12.647949"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..346
                     /locus_tag="PLG52_09220"
     CDS             83..346
                     /locus_tag="PLG52_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41668.1"
                     /translation="MQTPAQYAPCPQCGQSNAKPVNYTWWGGAIGPRMMSHVKCQSCN
                     TQYNGKTGKSNTQNIIIYNLVVFVVVFCACGGFAVLTSILNNQ"
     gene            458..649
                     /locus_tag="PLG52_09225"
     CDS             458..649
                     /locus_tag="PLG52_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB41669.1"
                     /translation="MTMKITEDCIACGACLPDCPTESITEGDGITYVIDQATCVECEG
                     HFDSPKCASVCPVECIVKA"
     regulatory      694..854
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1026..1802
                     /locus_tag="PLG52_09230"
     CDS             1026..1802
                     /locus_tag="PLG52_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41670.1"
                     /translation="MKPKTLHTLSRWLGIASLLLAIPLWALIVRISGLPKFILPSPAD
                     VWMRFIKALSDGSLLYHTWVTLAEILLGLIVGVCFATIVGYLLAKSRTLETVLSPYLV
                     ASQAIPVVAIAPLLVIWLGDGILSKVIICALIVFFPVLVNTIVGVRAVSASLYDLMNS
                     LRATRWQILWKLELPASLPILFGGLRIGATLSVIGAIVGELVDAQEGLGFLLQLGDFQ
                     YDTSMVFVAVFMLVFLALCLYGVVTLLERRFLKWQQRSNV"
     gene            1839..2816
                     /locus_tag="PLG52_09235"
     CDS             1839..2816
                     /locus_tag="PLG52_09235"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB41671.1"
                     /translation="MKKVVLLMLTLAVALMGCTSSQSTNAAGELRHIKLPMGYIPNIQ
                     YAPFYVAIDKGYFAEEGIEIEFDYSFETDGVALTGAGELPFAVASGEQVLLARSQGLP
                     VVYAFAWYQQFPISIISAPELNINEPADLRGKKIGLPGLFGANYIGLEALLFEGGVDP
                     SEVSMDAIGFNQVEAYASGNSDIVVGYAANEPIVLASQGFDLKEMRVADYVDLIANGI
                     VTNEDTIANDPELVRGMAKALARGIEATINNPEEAYDISLKFVENLKDQDKAVQMQVL
                     NTSIEFWKADRIGYSNPQAWENMNNVLVKMALIPEPIDLSKAFTNEFIP"
BASE COUNT          768 a          741 c          686 g          834 t
ORIGIN      
        1 attttttcat accaatggtt gattgctcaa tctctcttgt cttataaaat aaggctatat
       61 cttacactca ggagaaaatc tcatgcaaac cccagctcag tacgcccctt gcccccaatg
      121 cggacagtcc aacgctaaac cggtcaacta cacctggtgg ggcggtgcca ttggtccgag
      181 aatgatgagc catgtcaaat gccaaagctg caatacacaa tacaatggca agaccggcaa
      241 atccaatacc caaaacatta tcatctacaa tcttgtcgta ttcgtggttg ttttctgcgc
      301 atgtggtggg tttgcagttt taacttccat cctcaacaat caataagcaa cgcatgacaa
      361 actctccatg aattggagat gttcgtcact aaaacttctg tgatgcttca tgtacagtta
      421 catactgcaa gaaaaaatcc attcaggagt aactcccatg accatgaaga tcacagaaga
      481 ttgcatcgcc tgcggcgcat gcctgcccga ttgccccacc gaatccatca ccgaaggcga
      541 tggcatcacc tacgtcatcg accaagccac ttgtgtcgaa tgcgaaggtc acttcgactc
      601 acccaagtgc gcctccgttt gcccggtgga atgcatcgtc aaagcctaat tgaatcctaa
      661 tcttgttgag ggtataatca cctcaactga atattcaacc ttcggggcag ggtgacacgc
      721 aacccgcgtg aattcccgat cggtggtaca gcccacgagc ctgtttaggt acatcgcttt
      781 cctcattggg aaaacgggca tgatccggcg caactccgga gccgacagta tagtctggat
      841 ataagaaggt ataagcacaa ccccttgtgg gatctgtgtg gattctcacc ccgaaaacgc
      901 tctttgtttt cggggttttt atttatggaa ttcacgcgcc tcacgcctgg attgttgcct
      961 gttttcacgc cccggagttt tatgtactct gaggcgtttt tatttgccac ctgtgactga
     1021 tgatcatgaa accaaaaact ctgcacacct tatctcgctg gctagggatt gcttcccttc
     1081 tacttgcgat tccgctctgg gcacttatcg ttcgcatcag cggtcttccc aaattcatcc
     1141 tgccctcccc cgctgatgta tggatgcgtt tcataaaagc actgagtgac ggcagtttgc
     1201 tttatcacac atgggttacg ctggcagaaa ttttgctggg cttgatcgtt ggcgtttgtt
     1261 ttgccacgat cgttgggtat ctcctcgcta aatcgcggac acttgaaact gtcctttcac
     1321 cgtatttagt agcgagtcag gcaattcccg ttgtagccat tgctccctta ttagtcatct
     1381 ggctcggaga tggcattctc tcaaaagtta tcatctgtgc cctaatcgta ttctttccag
     1441 tgttggtcaa tacgattgtc ggggtacgag ccgtatccgc ttccctatat gacttgatga
     1501 actccctccg tgctacccgt tggcaaatct tatggaagct cgaattgcca gcctcgcttc
     1561 ccatcctctt tggcggactt cgcatcggcg caacgttatc agtcattggt gcaattgtcg
     1621 gtgagctcgt tgatgcgcaa gaaggtctgg gcttcctgtt acaactcggc gattttcaat
     1681 acgacacgtc tatggtcttt gtagcagtct ttatgttggt ctttctcgcg ctgtgcttgt
     1741 atggcgttgt caccctctta gaaagaagat ttttgaaatg gcaacaacgt tccaacgtat
     1801 aaacgtttga acttgaaaac atcttaggag acattgtaat gaagaaagta gttttactca
     1861 tgctaacgct ggcagtcgct ctaatgggct gtaccagctc acagtccacg aatgcggcgg
     1921 gggaacttcg tcatattaaa ttgccgatgg gctatattcc caacatccaa tatgcaccat
     1981 tctatgtagc aattgataaa gggtattttg cagaagaagg aattgaaata gagttcgatt
     2041 attctttcga gaccgatggc gttgcattga cgggggcagg cgaacttcca ttcgcagtgg
     2101 catcgggcga acaagtttta ttggcacgtt cacaagggtt accggttgtg tatgcttttg
     2161 cttggtacca gcaatttccc atctcaatca tctctgcacc agagttgaac atcaatgaac
     2221 ccgcagacct gcgcggaaag aaaattggct taccaggttt gttcggcgca aactacattg
     2281 gtttggaagc attgttattc gaaggcggag tagacccatc tgaagtaagt atggatgcca
     2341 ttggtttcaa ccaagtggaa gcttatgctt cgggcaatag tgacattgtc gttggctacg
     2401 cagcgaacga accgatcgta ttagcatctc aaggttttga cttaaaagaa atgcgtgtgg
     2461 cagattatgt tgacttgatc gcaaatggca ttgtgaccaa tgaagatacc atcgcgaacg
     2521 accctgaact tgtgcgcggc atggcaaaag cactggcacg cggcattgaa gctacgatca
     2581 acaaccctga agaagcctat gacatcagct tgaagtttgt agagaatcta aaagaccaag
     2641 ataaggctgt gcaaatgcaa gtcttaaaca catcgattga attttggaaa gcagatcgga
     2701 tagggtattc caacccacag gcgtgggaaa acatgaacaa cgtccttgta aagatggcgt
     2761 tgattccaga gcctattgat ctcagcaaag catttacaaa cgaattcatc ccataaggat
     2821 tttacgagtc gcggttcaca catcacgaca tgtgtgaacc gcgaattagc catgaccaca
     2881 aaacccatcc tcagtgttcg caacctttct gtcacttacc ccgataacaa tggcgggctt
     2941 cacgcgcttg ataacatctc gttcgaagtt cgtccgcgtg aatttgtttg ttttctgggt
     3001 ccttccggct cgggaaaaac gaccttctt
//
LOCUS       DAOQNJ010000316         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23257_length_3029_cov_9.249496, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000316 DAOQNJ010000000
VERSION     DAOQNJ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23257_length_3029_cov_9.249496"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1125
                     /locus_tag="PLG52_09240"
     CDS             127..1125
                     /locus_tag="PLG52_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41672.1"
                     /translation="MDFVVAFLKKLPVVPIVLFVVGLALGLLIGWGIAPVEFKDATPY
                     YLRPDLREDYMRMTIDSFSINKSAALAADRWLGLGSTAQEAYLAIQNNPGYLPSALVQ
                     EFGQAVTNEINARGGVPADPSTGMSPTMRTALIGIGVILLLGVVAAGGMYLFRLFGKR
                     GSGEVTVVMQAAEISRAAAKTNFEDMGLAPPITQTMTTYVLGDDLYDESFSIDTAAGE
                     FLGEYGVGVSEIIGVGEPKKVTALEIWLFDKNDIKTATKVLMSRHAFADPGIRSRLEP
                     KGELVLVEPQAQVLLETATLQLLATIVDIEYGKGPLPDSSYFERVTLELAIWPRQG"
     gene            complement(1170..1634)
                     /gene="greA"
                     /locus_tag="PLG52_09245"
     CDS             complement(1170..1634)
                     /gene="greA"
                     /locus_tag="PLG52_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNB41673.1"
                     /translation="MTQPNYLTPEGEAKLKAELEELKGPRREELAQRLRSAIQMGDLS
                     ENADYHKAKEDQGFLEGRIQEIEAILRNSVLIEKTASSDVVSIGSHVTIQEEDFDPET
                     YHIVGPTEADPRKGKISHESPIGVALMNKKVGQIAEAQAPGGKIKFKILKIE"
     gene            complement(1724..2875)
                     /locus_tag="PLG52_09250"
     CDS             complement(1724..2875)
                     /locus_tag="PLG52_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNB41674.1"
                     /translation="MFFTRQQLEENEDRSLAPYGMRSKDTKGRAYLDAEPEYRTTFQR
                     DRDRILHTTAFRRLEYKTQVFINFEGDHFRTRLTHTLEVAQIGRTLARALGGNEDLVE
                     AICLAHDLGHSPFGHSGETALARLMKDYGGFDHNKQSLRIVTELEQRYPEFPGLNLSW
                     EVREGMVKHESEYDISDARDFSPDLRGNLETQIANVADELAYTTHDLDDGLRSGMINP
                     QILEGVALWEILRETYNWHGSILSDMERHRMIRHLVGILVTDMVKGTDARLKESKVNS
                     ALDIQKLKHNVVGYTEEMQRRNRELKDLLYKKLYRHYRVVRMQVKAERIISDLFNAFR
                     AEPATLPDHVQFFIEKRGLERTICDYIAGMTDRYAIEEHQKMFNPNEKP"
     gene            complement(2942..3025)
                     /locus_tag="PLG52_09255"
     tRNA            complement(2942..3025)
                     /locus_tag="PLG52_09255"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2989..2991),aa:Leu,seq:taa)
BASE COUNT          687 a          687 c          776 g          879 t
ORIGIN      
        1 gtcgtagtgg caagtatggc tgagtctgat gcgttttttt atccatgtgg gcaatcattc
       61 tgtcatacag tgcccctaat ttacgttgta taatcgagcg aaagcatctg tgcgaaggag
      121 ccaattatgg attttgttgt tgcattttta aagaagttgc ctgttgtgcc aattgtcctt
      181 tttgttgtag ggttggcact tgggctgttg attggttggg gaatcgcgcc cgtagaattt
      241 aaggatgcaa ccccatatta tttgcgccct gatttgcgcg aagattacat gcgcatgacg
      301 attgactctt ttagcattaa caaaagcgca gcattagcag ccgatcgttg gctggggtta
      361 ggcagcacag cgcaagaagc ctatttagcg attcaaaaca accccggtta tttaccttct
      421 gctttggtgc aagagtttgg tcaggcagtg acgaatgaga tcaatgcacg cggtggcgta
      481 cctgctgacc cctctacggg tatgtctcct accatgcgca ctgcgttgat cggcattggt
      541 gtgattttgt tactgggtgt cgttgccgct gggggcatgt atctcttccg cttgtttgga
      601 aaacgaggct ctggcgaagt gaccgtagtg atgcaagccg ctgagattag ccgagcagcc
      661 gccaagacaa attttgaaga tatgggtctg gctccaccca ttacgcagac catgacaact
      721 tatgtcttgg gtgatgacct ttacgatgag tctttcagta ttgacacagc cgcaggtgag
      781 ttccttggtg aatacggcgt gggtgtttct gaaattattg gcgtgggtga gccgaagaag
      841 gttactgcgt tagaaatttg gctttttgat aagaacgaca tcaaaacagc gaccaaagtt
      901 ctgatgtctc gccatgcttt tgcagaccca ggaattcgct cgcgcctcga acctaagggt
      961 gagttggttc ttgtggaacc tcaagctcag gtgttgctcg aaacggcaac cctgcaactc
     1021 ctcgcaacca ttgtggatat tgaatatggc aagggacctt tgcctgatag cagctacttt
     1081 gagcgtgtga cacttgaatt agcaatttgg ccccgccaag gataaatata aacaaaaaaa
     1141 cgctccacat acgtggagcg tttttttgtt tattcaattt ttagaatttt gaacttgatt
     1201 ttgccgccgg gcgcttgtgc ttcagcgatt tgcccgacct ttttgttcat cagcgcaacc
     1261 ccaattgggg attcgtgaga gattttgcct ttgcgtgggt ctgcttcggt ggggccgacg
     1321 atatgatacg tctctgggtc gaagtcttct tcttgaatgg tcacatgaga gccaatggag
     1381 acaacatctg aggaggctgt cttttcaatt agtactgagt tgcgcaaaat ggcttcgatc
     1441 tcttgaatgc gaccttcaag gaagccttga tcttcctttg ccttatggta atcggcattt
     1501 tcggagaggt ctcccatttg gatggcagaa cgcaggcgct gtgccaattc ctcgcggcga
     1561 ggtcccttca gttcttcgag ctcagccttt agtttggctt caccctcggg ggtcaggtaa
     1621 ttgggttgtg tcatggtttc tcctttcctg atgtgcgttg gataagacga taaggggctt
     1681 gatacgaact ctatctctat gcccccttta gaaacgaaag aaattaaggt ttctcgttgg
     1741 ggttgaacat tttttgatgt tcttcgatgg cgtaacgatc tgtcatgccg gcaatgtaat
     1801 cacaaatggt gcgctccagt ccgcgttttt cgatgaagaa ttgcacatgg tctggcaatg
     1861 tggcaggctc ggcgcgaaaa gcattgaaaa ggtcagatat gatccgctct gctttgactt
     1921 gcattcgtac aacgcgataa tggcgataca actttttata gagcaaatct tttagctcgc
     1981 gattacgtct ttgcatttct tcagtatagc caactacgtt atgtttcagc ttttggatat
     2041 caagcgctga attgactttg ctttctttca agcgggcatc tgtgcctttg accatatctg
     2101 tgaccagaat tcctaccaag tgacgtatca tgcggtgacg ttccatatca ctgagaatgc
     2161 ttccgtgcca gttatacgtc tctcgcaaga tttcccaaag cgccacgcct tccaaaattt
     2221 gtgggttgat catcccagag cgtagaccat catcaaggtc gtgggtcgtg taagcgagtt
     2281 catctgccac attagcgatt tgggtttcga ggtttccacg taaatctggg ctgaagtcac
     2341 gcgcatcaga aatatcatat tctgactcgt gcttcaccat accttcacgc acttcccaac
     2401 tgagattcaa accggggaat tctgggtaac gttgttccag ttcggtcaca atgcgtaacg
     2461 attgcttatt gtggtcaaaa ccgccgtagt ctttcatcaa tcgcgccagg gctgtctcgc
     2521 cagagtggcc gaagggagaa tgcccaaggt cgtgtgccaa acaaatggct tcaaccaagt
     2581 cttcgtttcc accaagcgca cgcgcaagcg tccgcccaat ttgggcaact tcaagtgtat
     2641 gggtcaggcg ggtgcggaag tgatcccctt cgaaattgat aaagacctga gtcttgtatt
     2701 caaggcggcg aaatgccgtt gtatgcagaa tacgatctcg atcgcgctgg aatgtcgtac
     2761 gatattccgg ctctgcgtcg agataagcac gtcccttggt atctttacta cgcatcccat
     2821 agggagcaag gctgcgatct tcgttttctt ccaattgttg gcgtgtaaag aacatggtct
     2881 gtccttagat gcaaatgtca ttgcaacaat ctgtgttgag tgttgcaatg acagtggaga
     2941 gtggggcgag agggggtcga accctcacgt tgtttccaac gacagatttt aagtctgttg
     3001 cgtctgccga ttccgccatc gccccggca
//
LOCUS       DAOQNJ010000317         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23295_length_3026_cov_8.164591, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000317 DAOQNJ010000000
VERSION     DAOQNJ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23295_length_3026_cov_8.164591"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /gene="menC"
                     /locus_tag="PLG52_09260"
     CDS             complement(<1..1005)
                     /gene="menC"
                     /locus_tag="PLG52_09260"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HNB41675.1"
                     /translation="MKIESIILHHLSMPLIAPFETSFGRETDRQCIIVEVHSEGLVGW
                     GECVASYNPGYSYETVGTAMPTLKDFIIPLILGKDIKDALDFQSRMESIRGHHLAKAG
                     VEMAIWDLLGKRQGKSLKELFGGVRDKVEMGVSVGIQETPEKLVETVTNFVNSGYARV
                     KLKIKPGKDVTYAQAIRKAFPNIRLQVDANSAYSMDDADSLKPLDDLNLLLIEQPLFE
                     DDIWDHHKFQAKFSTPVCLDESIVSPRHARYAIEMKACRIINIKAGRLGGLSQGIQTH
                     DIAQQNGMPVWCGGMLETGIGRASNLALSSMPNFKLPGDVSASDRYYTRDITHERFVL
                     N"
     gene            complement(1053..1571)
                     /locus_tag="PLG52_09265"
     CDS             complement(1053..1571)
                     /locus_tag="PLG52_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNB41676.1"
                     /translation="MTFDPVFLANLTLVLTAFAGAFLVALWISLVVWTYRDIRSRARD
                     PLVQTLSALLVAVLNLPGVLVYLILRPPRTLEEEYQRTLEEEALLQALEDLPLCPGCE
                     RRVKEDWQICPNCHTKLKKTCHNCSKLMELPWNICPYCGTPAPGVRLEATSIDDALRG
                     IKIADEQGNNDN"
     gene            complement(1612..2916)
                     /locus_tag="PLG52_09270"
     CDS             complement(1612..2916)
                     /locus_tag="PLG52_09270"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNB41677.1"
                     /translation="MNFGSHRPYRHGTNLWLFTLFLAVSCAEPAPASTPDPTLVPLQV
                     NYQPTPTPFQPQPGTSPDPYLAISTPQDAVPTYTPVPTKYVPPQDVSVPVDVLPAAQP
                     GVTLYNPLTGLPVSDPYFLQHRPLAIKVGNSPDYVRPQSGLTLADVVYEYYIEWGDTR
                     FIAVFYSNLYNIERVGPVRSGRYFDENITRMYHSFLMFKGADPREYDYFMGLDIKQRF
                     ISVGIGKCPPYYYGHYKRDTYNNIFFNVTLWEACVAKKGLDNGPQTISGGFFSEEVPQ
                     SPLMVNKIYNFYTEYNYSYWQYDEETHSYFRYQESKDLVGYRKVEVYEQLFDDYTKEP
                     VTADNVVVLLVPYIFTNENQAEDEVYNPSLIDYGTAYVFRDGIAIPARWNRTAIDQPL
                     LLTNLDGTPIYLKPGQTFYQVMGVTSKQIQNGDEWRFEFATP"
BASE COUNT          777 a          658 c          804 g          787 t
ORIGIN      
        1 attgagaacg aaacgctcat gcgtgatatc gcgagtgtag taacggtcag atgcggagac
       61 atcaccgggc aatttgaaat tcggcatgga cgataaagca agattcgaag cgcgaccaat
      121 accggtctct aacatgccgc cacaccaaac gggcattccg ttttgttgag caatatcgtg
      181 cgtttgaatg ccttggctta atccgccgag cctgcctgct ttgatgttga tgatgcggca
      241 ggctttcatt tcgatggcat agcgagcatg gcgcggcgag acgatgcttt catcaagaca
      301 gactggggta ctgaacttgg cttggaattt gtgatgatcc caaatatcat cttcaaagag
      361 cggctgttca atgagcagta agttcaaatc atcgagcggt ttgagtgagt cggcatcatc
      421 catggaataa gcagagttgg cgtccacttg caagcggatg ttcggaaatg ctttgcgaat
      481 ggcttgggca taggtcacat ctttgccggg tttgattttg agtttcacgc gggcataccc
      541 gctgttgaca aagtttgtga cggtttccac cagtttttca ggtgtctctt gaataccgac
      601 ggatacaccc atttccactt tatctcgcac gccgccgaat aactctttga gtgatttgcc
      661 ttggcgtttc ccgagcaaat cccaaattgc catttctaca ccggctttgg caaggtgatg
      721 tccgcggatg ctttccatac ggctttgaaa atcgagggca tctttgatgt ctttgccgag
      781 aataagcgga atgatgaaat ccttcaaggt gggcatggca gtaccaaccg tttcgtaact
      841 ataaccaggg ttatacgaag caacacattc tccccaaccc accaaccctt ctgagtgaac
      901 ttctacgata atgcattggc ggtctgtttc acgcccgaac gatgtttcaa atggggcgat
      961 gagcggcatg gacaagtgat gcaagataat tgattcgatt ttcatggttt ctccaaagaa
     1021 gagaccctaa agctggcttt agggtcttga atttaattgt cgttatttcc ctgttcgtct
     1081 gcgatcttga ttcctcgtag ggcgtcgtcg atgctggtcg cttcgaggcg tacgccggga
     1141 gcgggggttc cacaataggg gcaaatattc cacggcagtt ccatcaactt tgagcagttg
     1201 tggcaggtct tcttgagctt ggtgtgacag ttcgggcaga tctgccaatc ttctttcaca
     1261 cgtctctcac agcccgggca aagtggaaga tcttccaagg cttgcaacaa ggcttcctct
     1321 tccaatgtgc gctgatattc ttcttccaag gtgcgtggcg gacgcagaat gaggtaaacc
     1381 aaaacgccgg gcaaattcaa cactgccact aacagagcag agagagtctg caccaagggg
     1441 tcacgcgcac gtgaacggat gtcgcgataa gtccacacga caaggctgat ccacaatgca
     1501 acaagaaatg cacctgcaaa agcggttaac acgagcgtga ggttggctag aaagacggga
     1561 tcaaaagtca tgagaactcc gaggtgggtt cattttaatg cattgggatg gttatggcgt
     1621 ggcgaattca aaacgccatt catcgccatt ctgaatttgt ttagaagtaa cgcccattac
     1681 ttgatagaag gtctgaccgg gtttcaggta aatgggtgtt ccatcgagat tggttagcaa
     1741 gagaggttgg tcaatggcgg ttcgattcca acgggcaggg atggcaattc catcgcggaa
     1801 gacgtaggca gtaccgtaat cgatcaagga tggattgtac acttcatctt cagcttgatt
     1861 ctcatttgtg aaaatatacg gcacaagcaa aacgactaca ttatcggcag tgacaggttc
     1921 tttggtgtag tcatcaaaca gttgctcata cacttctact ttacgatacc caaccaagtc
     1981 tttagactct tggtatcgga agtaggaatg ggtttcttca tcatactgcc aatagctgta
     2041 gttgtattcg gtgtaaaagt tgtaaatctt attaaccatc aacgggcttt gtggaacttc
     2101 ttcagagaag aatccgccgc tgatggtttg gggtccgtta tcgagtccct ttttggcgac
     2161 acaggcttcc caaagggtga cgttgaaaaa gatattgtta taggtgtcgc gtttgtagtg
     2221 cccgtaatag taaggtggac attttccaat accgacagaa ataaaacgtt gtttaatatc
     2281 caaacccatg aagtaatcat attctcgtgg gtcggcacct ttgaacatca agaaggaatg
     2341 atacatacgc gtgatgttct catcgaaata acgccccgaa cggacgggtc ccacccgctc
     2401 gatgttgtag agattgctat agaagaccgc aataaaacgg gtatcccccc attcaatgta
     2461 gtattcatag accacgtctg caagggtcaa gccagattgc gggcgaacgt aatctgggga
     2521 attccccact ttgatggcaa gtggacgatg ttgcaggaag tatgggtctg acactgggag
     2581 cccggtcaac gggttgtaga gggtcacacc gggttgcgca gcaggcaata catctacagg
     2641 gacggataca tcttgaggcg gaacatattt ggttgggaca ggtgtatatg tagggacggc
     2701 gtcttgcggg gtgctgatgg caagataagg gtctggagat gtgcccggct gaggttgaaa
     2761 aggcgtcggc gtaggttgat aattaacttg aagaggaaca agcgtcgggt cgggggttga
     2821 ggcgggggca ggctctgcac aagaaacggc gaggaagagg gtgaaaagcc agagatttgt
     2881 tccatgccga tatggtcggt gacttccaaa attcatggca gggattgtac caaaccacaa
     2941 ggtatcagcg cgacaatcat aatactgtta caattatgtt ttcccatgtt gatcttggtt
     3001 acctgtcttt tgctttttat tacagc
//
LOCUS       DAOQNJ010000318         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23397_length_3019_cov_7.177800, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000318 DAOQNJ010000000
VERSION     DAOQNJ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23397_length_3019_cov_7.177800"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLG52_09275"
     CDS             <1..554
                     /locus_tag="PLG52_09275"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNB41678.1"
                     /translation="QLKRVMETILLSAEFKASWGGKVKRPFEAAISMIRALNSETVRI
                     SGKLNWMLSLMGQAIFERRTPDGYPDTRDAWANSMSLLYRWNLAVSLAENWMDDDDTG
                     FKIASDVYGQTPAEIRSASSLADYWIQRILNRTLPDESRNAIIAVMAQEYGWDETLPE
                     DHVHYVLPAMVEVILMSPDFQWR"
     gene            559..1782
                     /locus_tag="PLG52_09280"
     CDS             559..1782
                     /locus_tag="PLG52_09280"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNB41679.1"
                     /translation="MTTLLPRRQFLQGCSAAIAAMAGAKLTNLAFAKQDNPSDILVVI
                     FLRGGWDALNVVPPIDGDDRGFYEKARPDLKISSLLRLNDQFGLHPAMSPLYDLYQAG
                     KAGIIHSVGLNYDTRSHFDAQDYIELGTPGSKSTTSGWITRHLQTTGISSILPVVSTT
                     GQPMSLLNFVPTVSVSTPDEFSLWDNGLRDSHMNALRQLYAGESLIHRAGSRTLDALD
                     IVRPLNGNTYQPANGAYYNDDELGNQLKTIAQMIKLDAGLRVAAVDFGGWDTHEYQNS
                     GNEGYMADLLNSLASGLANFYLDLDSGYTEKLSVVVISEFGRRLVQNDSYGTDHGHGS
                     VMLTLGGNVNGGQVFGSFPGLHNDQLYDHADLAITTDYRSVLGELLSKRMGNADLESV
                     FPGFSGNYNPLGIYR"
     gene            complement(1827..2792)
                     /locus_tag="PLG52_09285"
     CDS             complement(1827..2792)
                     /locus_tag="PLG52_09285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB41680.1"
                     /translation="MQSLTDISKRDLEILFDHIQAAIALVDRDGNLISWNSAFDAGKR
                     QYPEVTNLKDLFLQRDAESLRMKLAEGVANHWAGEFAITETDAAVLCDFWLMPTVTEH
                     MLFIAERIESEADAENMVEKLRKQVKLFQVESEVSKKLARNKQIEMEAVLAQAQEVAH
                     VDPLTFLPNRRTIIKDLQSEVMRAERYHSKFCISVVDIDKFKSINDTYGHPVGDEVLR
                     HVALQLRDGIRHPDVVGRYGGEEFIILLPNSDNNAAAEQASRLCRQIRSSVVRSKDHD
                     IQVTISVGIAQFHIGEDSWHSLLKRADNAMFEAKSRGRDRWVVGD"
BASE COUNT          736 a          801 c          704 g          778 t
ORIGIN      
        1 accaactcaa gcgtgtaatg gagaccatcc tgctttcagc ggagtttaaa gccagctggg
       61 gcggaaaggt caaacgtccg tttgaggcgg caatatccat gatacgtgcg cttaactcag
      121 aaacggtgcg catttcaggc aaactcaact ggatgttgag tctgatgggt caagccatct
      181 ttgaacgccg cacacctgat ggctatcccg acacccgcga tgcatgggca aactccatgt
      241 cgctcttgta tcgctggaat ctggcggtaa gtttggcaga aaactggatg gatgatgacg
      301 ataccggctt caaaatcgct tcggatgttt atgggcaaac cccggcggag attcgcagcg
      361 cctcttctct cgccgattat tggattcaaa gaatcctgaa ccgtacgcta cccgatgaaa
      421 gccgcaacgc catcatcgca gtgatggcac aagaatacgg ttgggacgaa accctgcctg
      481 aagatcacgt gcactatgtt ctgcctgcca tggtggaagt gattctaatg agccccgatt
      541 ttcaatggag atagaccaat gaccacttta cttccacgcc gtcagttcct gcaaggatgt
      601 tcagccgcca ttgccgccat ggcgggcgca aagttgacca acctcgcttt tgctaaacaa
      661 gataacccaa gcgatatcct cgtcgtcata tttctacgcg gcggttggga tgcgctcaat
      721 gttgttccgc ctattgatgg agatgaccgt ggcttttacg aaaaagcccg ccccgatctg
      781 aagatatcca gtctattgcg cttgaatgac caatttggtc tgcaccctgc catgagtccg
      841 ttatacgatc tgtatcaagc cggtaaagca ggcatcattc actctgtggg gttgaattac
      901 gatacgcgca gtcacttcga cgctcaagac tatattgaat tgggaacgcc cggttccaag
      961 agcactacct cagggtggat cacccgtcac ttacagacaa ccggcatttc ttcgatcttg
     1021 ccagtggttt ccaccacagg gcagcctatg tcgttgttaa attttgttcc cacagtcagt
     1081 gtgagcacgc ctgatgaatt ctcattatgg gataacggct tgcgcgactc gcacatgaat
     1141 gcgttgcgcc aactctacgc cggggaaagc ctcattcacc gtgccggctc gcggacattg
     1201 gatgccttgg atatcgtccg cccgttgaat ggaaatacat atcaacccgc caatggcgct
     1261 tactacaatg atgatgaact tggcaatcaa ctaaagacca ttgctcaaat gatcaaactc
     1321 gatgccggct tacgcgttgc tgccgtggac tttggcggtt gggatacgca cgaatatcag
     1381 aacagcggca atgaaggcta catggcagat ttgctcaaca gtctggcgtc tgggttggca
     1441 aatttttacc tagacttaga ctctggatac accgagaaat tatcggttgt tgtcattagt
     1501 gaatttgggc gtcggcttgt tcaaaacgac tcctacggca ccgatcatgg acatggcagt
     1561 gtgatgctta cgcttggcgg taacgtcaat ggcgggcaag tcttcggttc cttccccggt
     1621 ttgcataatg accaactata cgaccatgct gaccttgcca tcacaacaga ttatcgctct
     1681 gtattgggag agttactttc caaacgcatg ggcaatgccg accttgagtc tgtcttcccc
     1741 ggcttttctg gcaattacaa cccgctcggg atttacagat aactatttga aaaagagcct
     1801 atctcaatga gacaggctct ttttatttaa tctccaacga cccagcggtc acgtccgcgg
     1861 cttttggctt cgaacatggc attgtctgcc cgtttgagca agctatgcca tgaatcctcg
     1921 ccgatgtgaa actgagcaat ccccacactg atggtcacct gaatatcatg gtctttcgag
     1981 cggaccacag aactgcgtat ctgtctgcac aaacgtgagg cttgttcagc cgcagcattg
     2041 ttatctgagt ttgggagcag aatgatgaac tcttcgccgc cataacgtcc caccacatcg
     2101 gggtgacgga tgccatctcg caattgaagt gccacatggc gtaacacttc atcccctact
     2161 gggtgcccat atgtatcatt aatggactta aatttatcaa tatccacaac tgaaatacaa
     2221 aacttagaat gatatcgttc agcacgcatc acttcacttt gcaagtcttt gatgatggta
     2281 cggcgattcg gcaggaaggt cagtgggtcg acatgcgcta cttcttgcgc ctgcgccaag
     2341 accgcttcca tttcaatctg tttattgcgt gctagttttt ttgaaacttc gctttccacc
     2401 tgaaacaatt tcacttgctt gcggagcttt tccaccatgt tctctgcatc ggcttccgat
     2461 tcgatccgtt cggcgatgaa gagcatatgt tcagtcacag tcggcatcaa ccagaaatcg
     2521 cacaacacag cagcatcagt ctcagtaatg gcaaactccc cagcccaatg attggcaaca
     2581 ccttctgcca gtttcatacg caggctttca gcgtcgcgct gcagaaaaag gtcctttaag
     2641 ttcgtgacct ctgggtattg ccgtttcccc gcgtcaaatg cagaattcca cgaaattaaa
     2701 tttccgtccc gatccaccaa agcaatcgcc gcctgaatgt gatcaaacag aatttcaagg
     2761 tcacgtttgg aaatatctgt caaactttgc atagcataat tgtagcgtat tcgcctaccc
     2821 ttttatatgt ccttacaaaa ttaccaactg attaacgcgc ccctctgcgt ccaaattggc
     2881 gttccaacgt atcgaccgat aagtggatca tggtcggtct gccatgcgga cacgtccgcg
     2941 gcgattggca attctccaga tcaagcagca gactcttctg ctcgtcggat gtgagcgcct
     3001 gcccgccttt gaccgccag
//
LOCUS       DAOQNJ010000319         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23463_length_3014_cov_9.993241, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000319 DAOQNJ010000000
VERSION     DAOQNJ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23463_length_3014_cov_9.993241"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /gene="arsB"
                     /locus_tag="PLG52_09290"
     CDS             <1..787
                     /gene="arsB"
                     /locus_tag="PLG52_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNB41681.1"
                     /translation="ILMFVLAIIFLRGYPEYMTGLILIGLARCIAMVIVWNELAKGDS
                     EYAAGLVAFNSIFQVLFYSVYAYVFITVLPTWFGMTGNIVDITIGEIAKSVFIYLGIP
                     FLAGFFTRFFLVRAKGKDWYHKVFVPKISPMTLIALLFTIVVMFSLKGDVIVQLPLDV
                     VRIAIPLLIYFVVMFLVSFFMGKSIGADYSKTTTLSFTAASNNFELAIAVAVAVFGIG
                     SGAAFAAVIGPLIEVPVMIGLVNVAFFFQKRYFTSTVQHAPAR"
     gene            963..2213
                     /locus_tag="PLG52_09295"
     CDS             963..2213
                     /locus_tag="PLG52_09295"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB41682.1"
                     /translation="MNTKQKPHVLIIGAGFAGLETARGLAKAPVRITLIDKNNHHLFQ
                     PLLYQVAIAGLLPSQIAQPVRTIFRKQKNLAFQMGEVTDINLNEKFVKMNGSVIAYDY
                     LVIAAGARTNFFGFDTLEKHGLQLKDLETAVTTRNHLLSRFEGASHEADPEKRKAMLT
                     FVIVGGGPTGVETAGALAELITHVMRKDYPRLNLNEAQVILLEAGPHLIAPYPDELRQ
                     ATLRLLRDKKVDVRLNTKMQDFNGQRVTLGDGSVIESQTLIWTAGAKAAEVIDALPAE
                     KASMGRVRINPTLSLPQFPEAFVIGDASFLANGNGQPLPMLSTVAIQQGQAAAKNIRL
                     MIEGKQPKAFHYKDPGLLATVGRNAAVARIFGISFSGFIAWVIWVFLHIYRIIGFRNR
                     LIVMFNWAWDYFFYDNQVRLITRE"
     gene            complement(2252..2974)
                     /locus_tag="PLG52_09300"
     CDS             complement(2252..2974)
                     /locus_tag="PLG52_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNB41683.1"
                     /translation="MDQYPLDNNPNLTPPLSTPKPASWSANDTWLGLALLLIVVAGYL
                     FALSRMEQNQSTNILYVATFEFLLLIPIAVIFLWRKVSWKELGFNRFNANQLALGCGL
                     LVGVYMLVIINNLVMISLGVTTQADVISELLGEINAPYLFAFTTVIVAPITEEMFFRG
                     FLFKGLREKYGWVNALMFSSMIFAIFHGQLATLLPTFLLGALFSYLYQRTDSVFPGMF
                     LHFTVNAMGALVLLFANQTGAL"
BASE COUNT          758 a          734 c          771 g          751 t
ORIGIN      
        1 gatcttgatg ttcgtacttg cgatcatctt cttgcgcggc tatccggaat atatgacggg
       61 cttgatcttg atcggtctcg cgcgttgtat cgcgatggtc atcgtgtgga atgagcttgc
      121 caaaggcgat agcgaatatg cggcgggatt ggtcgctttc aatagcatct tccaagtgct
      181 tttctacagt gtatatgcct atgtctttat taccgtcttg ccaacttggt tcggcatgac
      241 cggcaatatc gtggacatta ccattggcga gatcgcgaag agtgtcttca tttatctcgg
      301 catcccgttc ctagcaggtt tctttacgcg cttcttcctt gtccgcgcga agggcaagga
      361 ttggtatcac aaggtctttg tgccgaagat cagcccaatg accctcatcg cattgctctt
      421 cactatagtt gtgatgttct cgctcaaggg ggatgtcatt gttcaattgc cgcttgatgt
      481 ggtgcgtatt gctattccgc ttttgattta ctttgtggtg atgttccttg tctcattctt
      541 tatgggcaaa tccatcggcg cggattattc caaaacgacc acgctttcat tcacggcagc
      601 tagcaataac tttgaactgg caattgcggt ggctgttgct gtctttggca ttggctctgg
      661 cgcggcattt gccgcagtca tcggtccgtt gatcgaggtg ccggtgatga tcggattggt
      721 caatgtggca ttcttcttcc agaagcggta cttcacgtcg actgtgcaac acgccccagc
      781 cagataaaaa ctcactggtc gtatcaagcc tctcgttgaa atgaagcggg aggcttttcc
      841 gttaagtggg gcagtactaa catcctattg tgaaaatatt tacaaataag acagttttgg
      901 ttataataat ggtgataaat ttcacaatta gaaataaatc gcataacaac acagaggaaa
      961 ccatgaacac caagcaaaag ccccatgtct taattatcgg cgctggcttt gccgggctcg
     1021 aaaccgcccg cggactcgcc aaagcgcctg tacgcatcac ccttatcgac aagaacaacc
     1081 atcatctctt tcagccgctg ttgtatcagg tggcgattgc tggtttgttg ccatcgcaaa
     1141 ttgcccaacc cgtgcgcacg atattccgca aacaaaagaa cctcgccttc caaatgggcg
     1201 aagtgacaga tatcaatttg aacgaaaagt tcgtgaagat gaacggctcc gtgatcgcct
     1261 atgattatct tgtcattgcg gcgggagcgc gcacaaactt cttcggcttt gatacgctcg
     1321 aaaaacacgg cttgcaactc aaagaccttg aaaccgccgt caccacacgc aatcatctgc
     1381 tttcaaggtt cgagggtgcc agccacgaag ccgaccccga aaaacgcaaa gccatgctca
     1441 ccttcgtcat cgttggggga ggtcccaccg gcgtggaaac ggcaggcgca ttagcagagc
     1501 tcatcacaca tgtgatgcgc aaggattatc cgcgtttgaa tttgaacgag gctcaagtca
     1561 tcttactgga ggcaggtccg catttgatcg cgccatatcc cgatgaactg cgtcaggcaa
     1621 cgctgcggct tttgcgagat aaaaaagtgg atgtgcgctt gaacaccaag atgcaggatt
     1681 tcaacggtca gcgcgtgaca ctgggagacg gttctgtaat cgagagtcaa acgctgatct
     1741 ggacggcggg cgcgaaagct gccgaggtca ttgacgcgtt accagccgag aaagccagca
     1801 tgggtcgcgt tcgcatcaac ccaacgttga gcctgcctca gtttcctgaa gcctttgtga
     1861 tcggtgatgc ttcttttctc gccaatggaa atggtcaacc tttgcccatg ctttcgaccg
     1921 tggcgatcca gcaaggtcaa gccgcggcga agaacattcg tctcatgata gaaggaaagc
     1981 aacccaaagc gttccactac aaagaccccg gtctgctcgc cacggtggga cgtaacgccg
     2041 ccgtcgcccg catcttcggc atctccttca gcggattcat cgcctgggtg atctgggtct
     2101 tcctgcacat ctatcgcatc atcgggtttc gcaaccgtct gattgtgatg ttcaactggg
     2161 cgtgggatta tttcttctac gataatcaag tacgactaat cacgcgggaa taaagaatac
     2221 atcctcagag attttctctg aggatgtatt cttataaagc accggtttga tttgcgaaga
     2281 gcaagaccaa tgcccccatg gcgttgaccg tgaaatgtag aaacatgccc gggaagactg
     2341 agtctgtacg ctggtacagg taagagaaca aggcgccgag cagaaaagta ggcaataaag
     2401 tagcaagttg cccatggaaa atggcaaaga tcatcgagct gaacatcaag gcattgaccc
     2461 agccatattt ttcgcgcaag cctttgaaga gaaagccacg gaagaacatc tcttcggtga
     2521 taggcgcgac aatgacggtg gtaaaggcga agagatacgg ggcgttaatt tcgccaagaa
     2581 gctcggagat gacatcggct tgggtggtga cgccgagtga gatcatcacg agattgttga
     2641 tgatgaccag catatacacg ccaactaaca acccgcaacc caatgccaat tgattggcat
     2701 tgaaacggtt gaagcccagc tctttccatg agactttgcg ccataagaag ataacggcaa
     2761 tgggaatcaa taaaaggaat tcgaaggtgg caacatacaa gatgttggtg ctttgatttt
     2821 gttccatgcg agaaagtgca aataaatacc cggcaacgac gatgagcaga agagccagcc
     2881 cgagccaggt gtcgtttgca gaccaagagg caggcttggg cgtggaaaga ggcggcgtga
     2941 ggttcgggtt attgtccagc gggtattgat ccatttgttt tttccaattc tctatacatc
     3001 agccattgac cttt
//
LOCUS       DAOQNJ010000320         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23464_length_3014_cov_7.770531, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000320 DAOQNJ010000000
VERSION     DAOQNJ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23464_length_3014_cov_7.770531"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLG52_09305"
     CDS             complement(<1..466)
                     /locus_tag="PLG52_09305"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNB41684.1"
                     /translation="MQPTPLLYPWLLSVTLTFLTGLYAFRYRRHVAAIPFILMCFFAS
                     FWALCYIFELGATSLELKTWGVKLGYIGILGLPLSWTAFALAYSGHTSWITKRNIMLA
                     LIFPIITLIVIFTNQYHHWFFTDFGLQTDSASGLILIYDPLGWWFWLHAVYTY"
     gene            639..2804
                     /locus_tag="PLG52_09310"
     CDS             639..2804
                     /locus_tag="PLG52_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB41685.1"
                     /translation="MSTTVMQPGLPGKSRSKVVTKPATDFYGGIGWVIGLLLTLFVSA
                     PVIWFLTQNASRADRADILAYGLAAAWWLNPLSLGGLFYVFSNIRMWRFWTLPALFVW
                     LLINAMVSASIASDVAREWVLNALFIGGLVILGLLFLYIQLRVFVWAASGNASRFFFG
                     IIGWALLNGLVVWALVNPDVLNIVLVALGFIFRVVFAIGFAIIQFVAIFWFMSQTRVE
                     IIRPGDPKQITFDDYKGQPNLLKMVRQWIYLLSDREQFQKMGGQFITGLLLYGEPGTG
                     KTLLAKAMAGEAGIAFISIEGSGFRGMFWGVDVLRMVQFVGRARKLAREYGACIAYID
                     EIDAVGMSRGSVMGGGQAMGGMMGMGAGSGALTRLLYEMDGIGTLTPWEKLRARVYQM
                     LRRKAPVRNWHVMFMGSTNRPDVLDPALVRPGRFDRKIAVGLPDRGGRREIVKYYLSK
                     IKHDETVDIEALVSDTSWATPARIMSAVTKDAVRLALFDGRDKISQRDIELAFQEQAI
                     GLENPIEEMEEDQRRQVAYHEAGHAVAQYHLRPDERIVRVSIIRRADALGYVLPVSNF
                     DVYAMPLRRFVADIMVSMAGHVATKVFFGEYWTGATGDFQNVRARLWQLAHYGYFGPP
                     ITMDSAMGAEGGYNGRGKIVEKFWQQLEDQTEQLLRTHRIEVEAVAQALLERGDLTGR
                     ECVEIIRQASGEGQADKTETDKMLSALVSDVTGEKPEEK"
BASE COUNT          726 a          685 c          802 g          801 t
ORIGIN      
        1 cataggtata aacggcatgc aaccaaaacc accaacctaa aggatcatag atcagaatta
       61 atccagaagc gctatcggtt tgtaagccga aatctgtgaa gaaccagtga tggtattgat
      121 tggtaaagat cacaatgagc gtgataatgg gaaaaatcaa cgccagcatg atgttgcgtt
      181 tagtgatcca gcttgtgtga ccagaatacg ccagtgcaaa agccgtccat gaaagtggaa
      241 gaccaagaat gccgatatac cccaatttca caccccatgt ttttaattcc aagcttgtag
      301 cgcctaattc aaagatataa caaagcgccc aaaatgatgc gaagaagcac atcaaaatga
      361 acggaatagc agctacatgc ctgcggtaac gaaaagcata tagtcctgtt aggaaagtaa
      421 gcgttacaga cagtagccaa ggataaagta gaggtgtggg ctgcatttga acaattatac
      481 aacttgatac cggggtaaaa gaattgcggg aacataatta ggcgaaagcg taagaatcac
      541 cctttttgtg catccaagaa ctaatagcca gctcttctca tttctttatc acgttttcaa
      601 gataaacttg ccccaatctt ctttcgctca gaggtctcat gtccacaacc gttatgcaac
      661 cgggcttacc cggtaagtca cgttccaaag tggtaaccaa gcctgccact gatttttatg
      721 ggggaattgg atgggtcatt gggcttttgc tcactctttt cgtttcagct ccagtcattt
      781 ggtttttgac gcagaacgcc tcgcgcgccg accgggcaga tatcctcgcc tatggcttgg
      841 cagccgcctg gtggcttaat ccgctatcgc tcggcgggtt gttctatgtt tttagcaaca
      901 ttcgtatgtg gcgcttttgg acgctccccg ctctgttcgt ttggctattg atcaatgcca
      961 tggtgtctgc gtccattgct tctgatgtgg cgcgtgaatg ggtcttgaac gcccttttca
     1021 ttggcggttt agtcattcta ggtttgctgt ttttatacat tcaattacgc gtatttgttt
     1081 gggcggcatc tggcaatgcc agccgctttt tcttcggcat tattggttgg gctttactta
     1141 atggcttggt tgtttgggca ttggttaacc ctgatgtgct gaatattgtt cttgttgcct
     1201 tgggctttat tttccgcgtt gtatttgcca ttggcttcgc catcatccaa tttgtggcga
     1261 tcttctggtt catgtctcaa acacgcgtgg agatcattcg ccccggcgac ccgaagcaga
     1321 ttacctttga tgattacaaa ggtcagccga acctgcttaa gatggtccgc cagtggattt
     1381 atctattgtc tgaccgtgaa caattccaaa aaatgggcgg acaattcatc accggcttgt
     1441 tgctctatgg tgaacctggc acaggtaaaa ccttgctagc caaagccatg gcaggggaag
     1501 caggcatcgc ttttatctcc attgaaggct ctggtttccg tggcatgttc tggggtgtgg
     1561 atgtcctccg catggttcaa ttcgttgggc gtgctcgtaa gcttgcacgt gaatatggcg
     1621 cttgtatcgc ctatatcgat gaaattgatg ccgtgggtat gtctcgtgga agtgtgatgg
     1681 gcggcgggca ggcgatgggt ggcatgatgg gtatgggcgc cggctctggc gcgttgaccc
     1741 gcctcctcta cgaaatggac gggattggca cgttgacgcc gtgggaaaaa ctgcgtgcac
     1801 gtgtctatca aatgttgcgc cgtaaagcac ctgtccgcaa ttggcatgtg atgttcatgg
     1861 gctctaccaa ccgccccgat gtgcttgacc cggcattggt gcgccccggt cgttttgacc
     1921 gcaagattgc tgtaggtctg ccagaccgtg gtggtcgccg tgagatcgtg aagtactact
     1981 tgagcaagat caaacacgat gaaaccgtag acattgaagc gttggtcagt gacacgtctt
     2041 gggcaactcc cgctcgtatc atgtctgcag tgacaaagga tgcggtccgt ttggcattgt
     2101 tcgacgggcg cgataaaatc tctcagcgtg atattgaatt agccttccag gaacaagcga
     2161 ttggtcttga aaaccccatt gaagaaatgg aagaagacca gcgtaggcaa gtggcatacc
     2221 atgaagctgg gcatgctgta gcgcaatatc acttacgccc cgatgaacgc attgttcgcg
     2281 taagtattat ccgccgtgcc gatgcgttag ggtatgtgtt gcccgtctca aattttgacg
     2341 tgtatgccat gccgctgcga cgctttgtag ccgatattat ggtctctatg gctgggcacg
     2401 tagctacgaa agtcttcttt ggcgaatact ggactggtgc cacaggcgac ttccaaaacg
     2461 tccgcgcaag gttgtggcag ttggcacatt atggttattt tggtccgcca attactatgg
     2521 acagtgccat gggcgcagag ggcggttata acgggcgtgg caagattgtg gagaagttct
     2581 ggcagcaatt ggaagaccag accgagcaac tcttgcgcac ccatcgcatt gaagtggaag
     2641 ccgttgccca agctttgctc gagcgcggtg acctgaccgg gcgcgaatgt gtggagatta
     2701 ttcgccaggc ttccggcgaa ggtcaggctg acaagactga gacagataaa atgctcagtg
     2761 cactggtcag tgatgtgacg ggtgaaaagc ccgaagaaaa gtaaacagta acaaaaaacg
     2821 ccctcgaaaa tcgagggcgt tttttattta acctatttcc aagaccatga agcgtataac
     2881 ccatccacaa gaaggcgcgg ctcggtaacg cctgatgatg gaatgatggc aatgcctgaa
     2941 ttgccattcg ggtctatgaa cgagatgacg atgttgttac tatctggcga ccagatcgtt
     3001 gtggattgat gata
//
LOCUS       DAOQNJ010000321         3013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23480_length_3013_cov_9.298174, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000321 DAOQNJ010000000
VERSION     DAOQNJ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23480_length_3013_cov_9.298174"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..515
                     /locus_tag="PLG52_09315"
                     /pseudo
     CDS             33..515
                     /locus_tag="PLG52_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
     gene            501..926
                     /locus_tag="PLG52_09320"
     CDS             501..926
                     /locus_tag="PLG52_09320"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNB41686.1"
                     /translation="MGKSVTHVGEAGAGQVAKAANQIMVAAQMVAMGELLVFAKKAGV
                     DPRKVVDAIKGGAAQCWTLDVKPPRLFDGNRNPGFKAHMQLKDMGIVLDTAKEYDIPI
                     SSAEEHTKFFKMMVEQGMGELDNAAVVGVIEKLAGVEIP"
     gene            complement(951..2456)
                     /locus_tag="PLG52_09325"
     CDS             complement(951..2456)
                     /locus_tag="PLG52_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNB41687.1"
                     /translation="MAIIKEFIRKIHAQQEKHKTWMDGLFLVLCVLYILSGIPLVPFH
                     GDESAYIILSEDYDRVFKKHEVEKVLFDPDGPKQYLRLSTGAILSFSIGFARDITNNE
                     DPINKWLWGASWDENLAKGNLPAPSLLNVARFSSAIMGALGTVLFFVLLRYLYPSRLV
                     AWLATLAFVTQGSLLVSIRRAMQEGPKFLFLTLTTLLAFLIIQNLQTQKLRKALYFAL
                     GIASGLTLAAKQDTAPVLVALYLTIAFIPVWNRTSFRAVWHTLLYLFAATLLAYATFL
                     AFMPVFWQWWESVFVLTGLSVALFQIPFIHETRSKVLASAGLALVVVMTFVSPALWGK
                     LHIPLSSMAELRQSIVGSQVEVSNGQDVLKADSAMGRLNFLLENTISSKVMYMEVDSF
                     NVPPMQASIKAYEASFLSGRTGSFVFDGLIFLLVLLGMWFLLRNFNSTSLLVYLLFFV
                     TGTFLLVAIPLAWQRYFIIMQLPYMLFAGAGVYQLEMWLKPLILEKKTASA"
     gene            2476..2979
                     /locus_tag="PLG52_09330"
     CDS             2476..2979
                     /locus_tag="PLG52_09330"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNB41688.1"
                     /translation="MEINQPVPDFELPDLNGALHRLSNYRGRIVIVNFWSCECPHSER
                     TDKAILSMFVQWQDDVTMLTIASNRSETVEALKEAAESRRLPNVLHDADCSVANLFDA
                     QTTPHVFVIDRDGILRYRGAVDDVTFRQRKPTRFFLDEAVEALLSGQFPTLTESPAYG
                     CAIVREV"
BASE COUNT          869 a          714 c          713 g          717 t
ORIGIN      
        1 cgttataatc tcaccattct cattcaagga gtctcttcat gttaagagtt ggttatatcg
       61 gtttaggatt aatgggcaaa tccattgcgc ggaatattat gaaggcgggc tttccggtag
      121 ttgttcataa tcgctcacgc gctgcggtgg atgaattggt cgccgaaggc gctactgcgg
      181 caggttcgcc caaagaagtt gccgaacaag tggatattgt ttttacaaat ttacccgact
      241 ctcccgatgt agaaaaagtg gcgttggatg aagttaatgg aatcattgca ggcgcaaaac
      301 ccggcacgat cgtggtggat aactccacca tcaaaccggc gtcggctcgc ttgatcgcgg
      361 cgaaattaaa agaaaaaggg attttctctc tggatgcacc cgtctctggc ggcgatattg
      421 gcgccaagaa tggtactctc actattatgg tcggcggcga agccgaagcg cttgaaaaag
      481 taatgcccgt ctttcaagcc atgggcaagt cggtcaccca tgtcggcgag gcgggagctg
      541 ggcaggttgc caaagctgcc aatcagatca tggtggcagc ccaaatggtg gcaatgggcg
      601 aattattggt ctttgccaag aaagcaggag tcgacccacg taaagtagtg gatgccatca
      661 agggtggcgc ggctcaatgc tggaccttgg acgtcaagcc accgcgtttg ttcgacggca
      721 accgcaaccc tggtttcaaa gcgcacatgc aattaaaaga tatgggcatc gtattggata
      781 cagcaaagga atatgatatc cctatctcat cggcagaaga gcacaccaag ttcttcaaga
      841 tgatggttga acaaggtatg ggcgagttgg acaatgccgc agtggtaggt gtgattgaaa
      901 aattggctgg ggttgagata ccctaataaa agcttttaca aaaacagcct ctacgcagag
      961 gctgttttct tttccagaat taagggcttc aaccacatct ctagttgata tacgccagcg
     1021 cccgcaaaaa gcatataagg aagttgcata atgatgaaat atctttgcca tgccagcgga
     1081 attgcaacca gtaaaaaagt gcctgtaacg aaaaacaaga ggtaaaccag aagactggtc
     1141 gaattgaaat tgcgcaaaag aaaccacatt cccaaaagca ccagcaagaa gataagtccg
     1201 tcgaacacga aagagccggt gcgcccgctc aggaatgaag cttcatacgc ctttatgctt
     1261 gcttgcatag gcggcacatt aaaactatcc acttccatgt acatcacctt ggatgaaatg
     1321 gtattttcaa gaaggaaatt cagcctaccc attgcagaat ccgccttaag aacgtcctgc
     1381 ccgtttgaaa cctccacttg agaccctaca atcgattggc gtaattctgc catactgctc
     1441 aatgggatat gtaatttccc ccataatgcc ggtgatacaa atgtcatcac aaccaccaat
     1501 gccagcccgg cacttgccag cactttcgaa cgcgtctcat ggataaaagg gatttgaaat
     1561 aaagctacag aaagaccagt taatacaaaa acactttccc accattgcca aaaaacgggc
     1621 ataaaagcaa gaaaggttgc atatgcaagc agagtggcag cgaaaagata taacaaggta
     1681 tgccaaacag ctcggaaaga tgttctgttc caaactggta tgaacgcaat cgtcaaatag
     1741 agcgccacca aaaccggagc tgtatcttgc ttggcggcaa gggttaatcc gctggcgatg
     1801 cctaaagcaa agtatagggc tttgcgcagt ttctgcgttt gaagattctg aatgatcaaa
     1861 aaagcaagca aggttgtcag ggtcaaaaac aaaaacttcg gaccttcttg cattgcccgc
     1921 cgaatgctaa ccaaaagact gccttgcgtt acaaaggcaa gggttgccag ccaggcaacc
     1981 aagcgagacg gatacaaata acgcaaaagc acaaaaaaca gcacagttcc caaagcgccc
     2041 ataattgcag aactaaacct ggcaacattc aacaagcttg gtgcagggag attccccttg
     2101 gcaaggtttt catcccaaga tgccccccac aaccacttgt tgatcgggtc ttcattgttg
     2161 gtaatatctc ttgcgaagcc aattgaaaat gacaatattg cccccgtaga taatcgcagg
     2221 tattgtttgg gaccatctgg gtcgaacaac accttttcta cttcatgctt cttaaagact
     2281 ctatcgtaat cttcgctcaa aataatataa gcagattcat ccccatgaaa tggcaccaaa
     2341 ggaatcccgc taaggatata gagtacacat aacaccaaaa acaaaccgtc catccatgtt
     2401 ttatgttttt cctgctgagc atggattttt cttataaatt cctttatgat cgccatgttg
     2461 aacattatat ctaaaatgga aattaaccaa cccgtccctg attttgaatt gcccgacctt
     2521 aatggcgccc tccatcgtct ttcgaattat cgcgggcgca ttgtaatcgt caatttttgg
     2581 tcgtgcgaat gtccgcattc cgagcggaca gataaagcga tactgtccat gttcgtgcaa
     2641 tggcaggatg atgtgaccat gttgactatc gcgtcgaatc gcagcgaaac ggttgaggcg
     2701 ttgaaagagg ctgctgaaag tcgccgcctg ccaaacgtct tgcatgatgc ggattgctca
     2761 gtcgcaaatt tatttgacgc gcaaacgacg ccgcacgttt tcgtcatcga ccgggatgga
     2821 atcctgcgct atcgtggcgc ggtggatgat gtgaccttcc gccagcgcaa gcccacgcgc
     2881 ttctttctgg acgaggcggt tgaggctttg ctctctgggc agttccccac gctgacggaa
     2941 tctcctgctt acggctgcgc cattgtgcgt gaagtttgat agaatccgaa cgtgatcgaa
     3001 ttcaaacaaa tcg
//
LOCUS       DAOQNJ010000322         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23493_length_3012_cov_7.233345, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000322 DAOQNJ010000000
VERSION     DAOQNJ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23493_length_3012_cov_7.233345"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PLG52_09335"
     CDS             <1..869
                     /locus_tag="PLG52_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB41689.1"
                     /translation="AGHNLFAFPAQSNFSSVKHPLEWIEKAHAHGWDVLLDAAAYVPT
                     SKLDLSKVKPDFVPISFYKIFGYPTGLGALIARKSALAKLHRPWFAGGTITVASVQGD
                     KYYLAEGAPAFEDGTLDYLNIPALEIGLKHIESIGYEVIGERVKTLTGWLLENLTAMK
                     HSTGEPLVRLFGPNSTEGRGGAVTVNFYDKNGKAFDHRFIESEANKVNISLRTGCFCN
                     PGAGEVALGISRVELDVCFTRPGNQDRLSIDDFRLCIDGKSSGAVRISVGPFTNFNDV
                     QALLSFARGLLS"
     gene            1075..1392
                     /locus_tag="PLG52_09340"
     CDS             1075..1392
                     /locus_tag="PLG52_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB41690.1"
                     /translation="MKTYYVYIMASKSRVLYTGVTNNLERRVYEHKNKLIEGFTNKYN
                     VDRLVHYEETNDVNVAITREKQIKGWLRSKKIALIESANPQWKDLSLEWYVSAHETNK
                     VSS"
     gene            1676..2071
                     /locus_tag="PLG52_09345"
     CDS             1676..2071
                     /locus_tag="PLG52_09345"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41691.1"
                     /translation="MASQIQALNHYRPQIEYGDTAGWREVAEFLEASSTLSKADIIAA
                     LTGLQEAVIHFNLQGRGVKLEGLGTYLPNINYQGELDAAHRLDRRLKRGLNTGSFQGK
                     IRNKKNIGKTVEQIVAMWNAEHPDDPVLY"
     gene            2095..2595
                     /locus_tag="PLG52_09350"
     CDS             2095..2595
                     /locus_tag="PLG52_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB41692.1"
                     /translation="MNIQNIHLMFEYNYWANGRIFGAAKHISPEQLLAPAEFPYGDLR
                     ATLVHIIDAEYGWRGLFENDKFNDEIKPEEFANVQSIVERAMAEEKAMRAYLSKLTDA
                     DVESHKKYIADNGEPRDRILWHCLYHVVNHGSQHRSEAAAILTHFNASPGDLDFTVFL
                     NEVKPK"
     gene            2676..2990
                     /locus_tag="PLG52_09355"
     CDS             2676..2990
                     /locus_tag="PLG52_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNB41693.1"
                     /translation="MSDQPARPIRFVVFHMPGPKWQQGVDYRQQAGVNEHVQHYFNLH
                     QQGKLQLGGPFLLPDMGGMMVTTKDVTYEEIDAFAAEDPAVQSGLLIYEIRPWMTAME
                     HE"
BASE COUNT          817 a          797 c          708 g          690 t
ORIGIN      
        1 aggctggtca taacctgttt gctttccccg cccaatcgaa cttctcctcc gtcaagcatc
       61 cgctcgaatg gatcgagaaa gcccacgccc acggctggga cgtgctccta gatgcagctg
      121 cctacgtccc caccagcaag ctggatctga gcaaggtcaa gcctgatttc gtaccgatct
      181 ctttctataa aatattcggc tatcctaccg ggcttggcgc actcatcgca agaaaatcag
      241 ccttggcgaa acttcatcgc ccctggttcg ccggtgggac gatcactgtc gcatcggtac
      301 aaggcgacaa atactatctc gccgaaggcg cacccgcctt tgaagatggc acgctcgatt
      361 acttgaatat tcccgccctt gaaattgggc tcaagcatat tgaatccatc ggctatgaag
      421 tgatcggcga acgagtcaaa acgctcactg gctggctgtt ggaaaatctc accgccatga
      481 agcacagcac cggcgagcct ctcgtgcgcc tgttcggacc caactccacc gaaggcagag
      541 gcggcgcagt gaccgtcaac ttctatgata agaacggcaa agctttcgat catcgcttca
      601 tcgaaagtga agccaacaaa gtgaacatct ctctccgcac cggctgtttc tgcaaccccg
      661 gcgcaggtga agttgccctc ggcatctccc gcgtggagtt agacgtctgc ttcacccgcc
      721 ccggtaacca agaccgcttg tctattgatg atttccgcct ctgcattgac ggcaaatcct
      781 ccggcgcggt gcgcatctcc gtcggtccgt tcaccaattt caatgatgtg caagcgttgt
      841 tgtcgtttgc aagagggttg ctgagttagt gtcatgctga gcgacagcgc cgcgtccccg
      901 aagggggaag catctctacg ataatctcag agatccttcg gtcgcaaaga acgctccttc
      961 aggatgacat cattatttgt cgtgctaagc cgataggcga agcatctcta cacaatctca
     1021 gagatccttc ggccacaaag aacgcgccct caggatgaca aaagagaggg ttatatgaaa
     1081 acgtattacg tatatattat ggcaagtaaa tcacgcgtcc tttacacagg cgtgactaac
     1141 aatcttgaac ggcgtgtgta cgagcacaaa aataaactca ttgaagggtt tactaacaag
     1201 tacaatgtcg accgtctcgt ccattacgaa gaaacaaatg atgtaaatgt tgctattacg
     1261 cgtgaaaaac aaatcaaagg ttggttacgg tcgaagaaaa ttgcattaat tgagtcggca
     1321 aacccgcagt ggaaagacct aagtttagaa tggtatgtat cagcgcatga aacaaataag
     1381 gtgtcatcct gagccgatag gcgaaggatc tctaccataa ccctagagat tcttcggtcg
     1441 cggagaacac gcgccctcag aatgacaaga cacattgcat cccagacctc cgaggtttta
     1501 aaaacctcgg aggtctggtt ttccaaaaca tactgtccgt ttccccaacc tccactgtct
     1561 agttgacaat atcctgccag catatttata atatcctgca ataacataat caatatcctt
     1621 gcaggatatt aggattaaga caagtcttgt tcattaacaa gacaggagga attgcatggc
     1681 gtctcaaatt caagcactca accactaccg tccacagatc gaatatggcg ataccgctgg
     1741 ctggcgggaa gttgccgagt ttttggaagc cagctctact ttgagcaagg cagacattat
     1801 cgccgccctg accgggttac aagaggctgt catccacttc aatcttcaag ggcgcggggt
     1861 taagctcgaa gggctgggca cttatttacc gaatatcaac tatcagggcg agcttgatgc
     1921 ggcgcatcgt ctggatagac gattaaagcg tggactgaat acaggttctt ttcaagggaa
     1981 aattcgcaac aaaaagaata ttgggaaaac agtagaacag attgttgcaa tgtggaatgc
     2041 agagcatccc gatgaccccg tgctatactg aaaaagtacc tactggagaa gccaatgaac
     2101 attcaaaaca tccaccttat gttcgaatac aactactggg cgaacggacg catcttcggc
     2161 gctgcgaaac atatctcccc agaacaactg cttgccccgg cagaatttcc gtatggagat
     2221 ttgcgagcca ccctcgtcca catcatcgac gccgagtatg gctggcgcgg tttgtttgag
     2281 aatgacaaat tcaatgatga gatcaagccc gaagagttcg ccaatgtcca gtctattgtg
     2341 gaacgtgcca tggcagaaga aaaagccatg cgagcttacc tcagcaaact taccgatgct
     2401 gatgtggaaa gccataaaaa atatatcgcc gataatggcg aaccccgcga ccgcatcctt
     2461 tggcattgct tgtatcatgt ggtcaaccat ggctcgcaac atcgcagtga agcggctgcc
     2521 attctcaccc acttcaacgc ctctcccgga gacttggact ttacggtctt tctgaatgag
     2581 gttaagccca aataaacact acggcagaat ggtgcgtacc cggcaaaggg tcggtatgat
     2641 gcgggtttat catccaatac atataggagc ttcttatgtc tgatcaaccc gcgcgtccca
     2701 ttcgttttgt cgtcttccac atgcccggac ccaaatggca acaaggtgta gactatcgcc
     2761 agcaagctgg tgttaacgag catgttcaac attatttcaa ccttcaccag caaggcaaac
     2821 tacaactcgg cggacccttt cttttgcctg atatgggcgg catgatggtg accactaaag
     2881 acgtcaccta cgaagagatc gatgccttcg ccgccgaaga cccggctgta caatcaggct
     2941 tgctcatcta cgagatccgc ccgtggatga ccgccatgga gcatgaatga ccaacggaca
     3001 aacccgcgca aa
//
LOCUS       DAOQNJ010000323         2995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23711_length_2995_cov_9.846599, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000323 DAOQNJ010000000
VERSION     DAOQNJ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23711_length_2995_cov_9.846599"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="PLG52_09360"
     CDS             <1..1305
                     /locus_tag="PLG52_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41694.1"
                     /translation="KATDVSLQLGSAFTSPLTVLWWLNYLVQDVFKVIMVAWTVPLNL
                     FGFSLRLRDAFMGLGLAAIAVALLLFFLCQSNEKNGDDESGQSPSEMREQLWLAFITI
                     VACLVPVILVNRHVVLPDYSRYALAPSLGVGILFSVIFERVNTASLRSGLVAFLLGVS
                     VLTHHGNAVRVADETAAVQNFWWQVAWRAPKIQEGVTLIASFPGSPLAEDYFIWGPAN
                     FIYYPEKQTNNPLLIKLPAAVLDGNAVTQITTNGGVETPLRRGNYLERDFGNVLVMVQ
                     SSNDSCVRFIDGRSPELNSYDDGRVAMIASYSKLDSVVTEGDVSAVPEEIFGAEPERG
                     WCYTYQQADLARQRGDWDAIPVLLKDALDKGYYPNDGLEWMPFMQAYAVQGNVEEIKS
                     MLKLVVADKYLRLQVCNSLTNLMQRETLNGDVSALIEKNICK"
     gene            1323..2852
                     /locus_tag="PLG52_09365"
     CDS             1323..2852
                     /locus_tag="PLG52_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41695.1"
                     /translation="MKKFLSRLFNAPWYSIVFGAYPILALLSENKGQVEPSAIFRPLL
                     LSLSFSTIILLIAWLVLRKIHKAAFFSVLFLLLFFTYGHVYMYFEEKFPENTAVQWLW
                     VMWVILFVLSIAWVTRPKLTFETAASSLNGMILVLLIMSITQLDFNTSSGNSHRVGAR
                     NAPIQEDLVLPENPPDVYFFLLDSYGRSDLLQSAYGYDNSDFENALKERGFYIANESQ
                     SNYVRTEISLGSSLNMQYLQELDPAFNPQSIGRKVLWDALKHNAVRYNFDTLGYETIN
                     YASGFAWLEVTDSDQFISPPPISSGMTEFEGLFLRTTWARYAEKWGWIDSDAIMAEGY
                     RDRFNTIFNSMDSISKMPEPKFVYAHVISPHPPFVFDPEGNPTNPADFWNEKRQYTGE
                     MYKKGYLNQLPYLNTRMLEAIDTIMANSKTPPVIIIQGDHGPWLQPKDKRMWNFTAVY
                     MPGHEDTLYPTITPVNLFRLVFNNYFGGKYDILKDVSYFSPVPKLYEFTEIPNPYDEG
                     H"
BASE COUNT          739 a          652 c          712 g          892 t
ORIGIN      
        1 aaagcgaccg atgtcagctt gcaattgggc tctgccttta catctccgct gacagtctta
       61 tggtggttga attatcttgt tcaagatgtg ttcaaggtca tcatggtggc gtggacagtg
      121 ccactaaatc tctttggatt ctcccttcgt ctgcgtgatg cctttatggg gcttgggttg
      181 gcagccatag cagttgcttt gctgctattc tttttgtgtc agagtaatga aaagaacggg
      241 gacgatgaat caggtcaaag tccaagcgag atgcgggagc agttgtggct ggcgttcatc
      301 accattgtgg cgtgtctggt tccggttatc cttgttaacc ggcatgtcgt tttgccagat
      361 tattcacgct atgcgttagc gccttcttta ggcgtgggta tcttgttctc ggtcattttt
      421 gagcgtgtta acacggcttc tttgcgttct ggcttggtag catttttgtt gggcgtttcg
      481 gtgttgacgc atcatggcaa tgctgtgcgc gtggcagatg aaacagctgc ggtgcagaat
      541 ttttggtggc aggtggcatg gcgcgcgccg aagatccaag aaggcgtgac gttgatcgca
      601 tctttccctg gcagcccgtt ggcagaagat tattttattt ggggaccagc caattttatt
      661 tattaccctg aaaagcaaac caacaacccg ttgttgataa aactccctgc cgcagtattg
      721 gatggcaatg cggtgacgca gatcaccacc aatggcggag tggaaactcc gttacggcgc
      781 ggcaattatt tggagcgaga ttttgggaac gtgttggtga tggttcaatc aagcaatgac
      841 agttgtgtac gcttcattga tggtagatcg ccggaactga attcatacga tgatggtcgc
      901 gtggcgatga tcgcgtctta ttcaaaattg gactctgtag tgaccgaagg tgatgtgtct
      961 gctgtgccag aggaaatctt tggcgcagaa cccgagcgtg gctggtgcta cacctatcaa
     1021 caagctgatc ttgcccgcca acgaggtgac tgggatgcga ttccagtttt attgaaagat
     1081 gctttagata aaggctacta ccccaacgat ggtttggaat ggatgccatt tatgcaagcc
     1141 tatgccgtcc aaggcaacgt ggaagagatc aaatccatgc tgaagctggt ggtagcagat
     1201 aaatacttgc ggttgcaagt ttgtaattcg ctcacaaacc taatgcagag agaaactctt
     1261 aatggggatg tatctgcact gatcgaaaaa aatatctgta agtagatttt agaggtgtaa
     1321 cgatgaaaaa gttcctttca cggttgttta acgcgccttg gtattctatt gtttttggcg
     1381 catatcccat tttggcgttg ctctctgaaa acaaagggca ggtggagcca tccgctattt
     1441 ttagaccttt gttgctttcc ttgtcattca gtactattat ccttttgatt gcatggcttg
     1501 tgcttcgtaa aatacacaaa gctgcgttct tttctgtttt gtttcttctg cttttcttta
     1561 catatgggca tgtatatatg tactttgaag agaaattccc tgagaataca gcggttcaat
     1621 ggctgtgggt gatgtgggtt attctttttg ttctttctat tgcctgggtt actcgcccca
     1681 agttgacttt tgaaacagcc gcctcttctt tgaatggcat gattttggtt ttgttgatta
     1741 tgtccattac tcaattagat ttcaatacat cttcaggaaa ctcgcatcgg gtaggcgcaa
     1801 gaaatgcgcc catccaagaa gaccttgttt tgccagaaaa cccacctgat gtatattttt
     1861 ttctgctaga ttcatatgga cgttcagact tactccagtc tgcatatgga tatgataata
     1921 gcgactttga aaatgctctg aaagaacgtg ggttttatat cgccaacgaa agccaatcga
     1981 actacgtccg cacagagatt tcattgggtt cttcgctcaa tatgcaatac cttcaagagt
     2041 tagacccagc attcaacccg caatcaattg gcagaaaagt attatgggat gctctaaagc
     2101 acaatgcagt gcgttataac tttgacaccc ttgggtatga aacgattaac tacgcttctg
     2161 gctttgcctg gctagaggtt acagattcag accagtttat aagtccgccg cccatttctt
     2221 caggcatgac cgaatttgaa ggattgttct tgcgcacaac ttgggcgcgc tatgccgaaa
     2281 aatggggctg gatcgactca gatgccatca tggcggaagg ctatcgcgac cgcttcaata
     2341 ccatcttcaa tagcatggac tctatttcaa aaatgcctga gccgaaattt gtctatgctc
     2401 atgtgatttc accgcatccg ccttttgtgt ttgaccctga aggcaaccca accaaccccg
     2461 ctgatttttg gaacgagaag cgccaataca caggtgagat gtataaaaaa ggctacttga
     2521 atcaattgcc ctatctcaac acgcgcatgc tcgaagccat tgatacgatt atggcaaatt
     2581 ccaaaacgcc gccagtgatt attattcagg gcgatcacgg tccgtggttg caacctaaag
     2641 acaagcgtat gtggaatttc acggcggtat acatgcccgg tcatgaagat actttgtatc
     2701 ccaccattac accggtcaac ttgttccggc ttgtcttcaa taattatttt ggcggcaaat
     2761 atgatattct caaagatgtc agttatttct cgccagttcc aaaactctat gagttcactg
     2821 aaattccaaa cccttacgat gaaggacatt gatcgatgaa gtacaccgtt ctactcacgg
     2881 ctccctacat gctccctttt gtggaccgtt tcaaacccgt cttcgactcc tacgatatcg
     2941 agctgattat tccgaatgtg gaagaacgca tggaggaaga agacatcctc aaata
//
LOCUS       DAOQNJ010000324         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23772_length_2991_cov_10.768052, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000324 DAOQNJ010000000
VERSION     DAOQNJ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23772_length_2991_cov_10.768052"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..2482)
                     /locus_tag="PLG52_09370"
     CDS             complement(173..2482)
                     /locus_tag="PLG52_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB41696.1"
                     /translation="MPGREDIFQKAMNEGHSAAWDQEWSKAVAAYRRALEESPDNPKA
                     LSSLGQALHQLNNVEEALQIYMRAVKVSPDDPVSMEKVAQLSERVGDLKTATEAAIRA
                     GDLFLKQRDTDKALENWVHVTSINPEHAIAHSRLAQVHERLGHGQQAAMEYLAIASIL
                     QRAGNPEKTQEMVNKAQSLVPNSAEVKQAQTLLRTGQLLPKPIRAKGGTGPIRMAQVK
                     QLQEPAKKAASGLDPIAEARQKALTQLAEVLFDLSDDSSPVAQDRKGISAIMRGTGSL
                     SAQSEQLKVVLHLGQAIDAQSKGNDTLAAEEMEAALNAGFSSSALSFSIGYLRFKNER
                     YESAQRALQNSVKHEDYGLASRLLLGDIISRKGDFRDAATQYLEALKFADAATVPLEQ
                     GDDIRQQYEPLIESYQQQTDEAVLRKVCDNIRGLLMRADWRDQLHKTRDQMPKQDGMA
                     APLADMILQVQSSSVLESMNRINQLARMGSLRSAMDEAYDAIQHAPTYLPLHIIMGDL
                     LVQEGHTTDAIAKFSVVAHSYSVRGEVMQASKLLRRIIQISPMDLGARNRLIDQLVAR
                     GQVDDAIQEYLELAAIYYRLAELDMARKTYTHALRIVQQGNASRDWNTHILQRMADID
                     MQRLDWKQALRVYEQIRTLMPDDDASRKQLIELNMRMGQQDRAMSELESYITHLESQN
                     KQELAIAFLEELARDHEDQPLIKRTLAALLHRSGRIGDAIPILDQLGEALLEKGDKQG
                     AMEVINQIVLMNPPNVQDYRVLLSQMQNS"
     gene            complement(2564..2788)
                     /locus_tag="PLG52_09375"
     CDS             complement(2564..2788)
                     /locus_tag="PLG52_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41697.1"
                     /translation="MSEIDKRNVLDNEVFTYKINKEKKVFIYWRGKQVLILRGSEAQK
                     FTSKIIGLDDKGVQLVLAKLTGNFKRGNER"
BASE COUNT          618 a          697 c          813 g          863 t
ORIGIN      
        1 tcatcacggc gcggttttgg gtttttggca tatgattact ccaatttgaa ttggcggcat
       61 tatagcattc aggacttagg agattgttag aacttttgtc acgaaagttg ctgtgatttg
      121 tcacccggga gaagcgtatc aaaaagcgtg gagttgctct ccacgctttt ggctaactat
      181 tttgcatttg gcttagcagt accctgtaat cttgcacgtt gggtgggttc atcagcacaa
      241 tctggttgat gacttccatg gcgccttgtt tatccccttt ttcaagcaag gcttcgccta
      301 attggtcgag aatgggtatg gcgtctccga tgcgaccgct acggtggagc aacgctgcga
      361 gcgtgcgttt gatcaatggt tggtcttcat ggtcacgtgc caactcttca aggaaggcga
      421 tggcaagttc ttgcttgttt tggctttcaa ggtgagtgat gtaactttcc agctcgctca
      481 tggcgcggtc ttgttgcccc atgcgcatat tgagttcgat cagttgtttg cgagaggcat
      541 cgtcatcggg catgagggtg cggatctgtt catagacgcg caaagcctgt ttccagtcca
      601 agcgctgcat atcgatatct gccatacgct gaaggatgtg ggtattccaa tcgcggctgg
      661 cattgccttg ttgcacaatg cgtaatgcgt gagtgtatgt cttgcgtgcc atatcaagtt
      721 cagcgaggcg atagtagatg gcggcaagtt caagatattc ttgaatagca tcatcaacct
      781 gaccacgggc tacaagctgg tcaatcagac gattgcgcgc gccgaggtcc atgggcgaga
      841 tttggatgat acggcgtaat aacttgctgg cttgcatcac ttcgccgcgt acactgtatg
      901 aatgtgccac cacgctaaac ttggcaatcg catcggtggt gtgaccttct tgtacgagca
      961 aatcgcccat gatgatgtga agcggtaggt aggtgggggc gtgttgaatg gcgtcatagg
     1021 cttcgtccat ggcagagcgc agactgccca tgcgtgccaa ctggttgatg cggttcatcg
     1081 actcgagcac cgagctggac tgcacctgca agatcatatc tgccagaggg gctgccatgc
     1141 catcttgctt gggcatttgg tcgcgggtct tgtgaagctg gtcgcgccaa tctgcacgca
     1201 ttaacagccc gcgaatgtta tcgcagactt tgcgcaaaac ggcttcgtct gtttgctgtt
     1261 ggtacgactc gatcaacggc tcgtattgct ggcgaatgtc atcgccttgt tctagtggaa
     1321 cggtagccgc atctgcaaac ttcaaggctt ccagatattg cgttgccgca tcgcggaagt
     1381 cgcctttgcg cgaaatgata tcgcctagta acaggcgcga ggctaaacca taatcttcat
     1441 gcttaaccga attttgcaaa gcgcgttggg cgctttcgta gcgttcattt ttgaagcgta
     1501 aatagccgat gctgaatgaa agagctgaac tgctaaagcc agcattgagt gcggcttcca
     1561 tttcttcagc ggcaagcgtg tcgttgcctt tcgattgcgc atcaatggct tgacccaaat
     1621 gcaaaaccac tttgagttgt tccgattgcg ccgaaaggga gccggttccg cgcatgatgg
     1681 cagagatgcc tttgcggtct tgcgccacgg ggctgctgtc gtcggagaga tcgaagagga
     1741 cttctgccag ctgagtcaac gctttctgac gggcttccgc gatcgggtcc agccctgagg
     1801 cggctttctt cgccggttcc tgcaattgct tgacttgtgc catgcggatg ggtcccgtgc
     1861 cgcctttggc gcgaattggc ttgggcaaga gttgaccggt tcttaataac gtttgggctt
     1921 gtttcacttc ggcgctgttg ggtaccagcg attgggcttt gttcaccatc tcttgcgttt
     1981 tttcggggtt gcctgcgcgt tgtaaaatgc tggcaatggc aagatattcc atggcggctt
     2041 gttgaccatg ccccaaacgt tcgtggactt gtgcaaggcg tgaatgggct atggcatgtt
     2101 cggggttaat gctggtgaca tgtacccagt tttcgagggc tttgtcggta tcgcgttgtt
     2161 tgaggaagag gtcacctgcg cggatggcgg cttcggtggc ggttttcagg tcgccaacgc
     2221 gttcagagag ttgcgccact ttttccatgg agactgggtc atctggcgag accttgacgg
     2281 cacgcatgta gatctgcaag gcttcttcca cgttgttgag ctgatggagt gcctgcccga
     2341 gactgctgag cgctttgggg ttatcgggtg attcttcgag ggcgcggcga taggcggcaa
     2401 ccgctttgct ccattcctga tcccaggcag cggagtgtcc ctcgttcatg gctttttgga
     2461 agatatcttc tcgtccgggc atagtgttgt tattttactt tcaaaatagg gtgggtcgtc
     2521 ctaacaatca tgcaattata accttattgg ttaggggcgt gcgctagcgt tcgttgccgc
     2581 gtttgaagtt ccccgtcagc tttgccagca ccagttgcac tcctttatcg tctagcccga
     2641 tgatcttgct cgtaaatttt tgggcttcgc ttccccgtaa aatcaggact tgtttccccc
     2701 gccagtaaat aaacaccttt ttctctttgt tgattttata ggtaaagacc tcattatcca
     2761 atacgtttcg tttatctatt tcgctcataa tgtctaaaga tcttttgtat tctatatggc
     2821 aaactagaat attaggatgc ccaaaggaag gctccatcaa gaaagagcat tatgaatatg
     2881 cttatacata ttataaaaaa taaaataaga cctgtcacaa tatagaataa aagccagtcg
     2941 gttgaatttt tcggactcat accaatcctt tatatacctg ctgtattttt t
//
LOCUS       DAOQNJ010000325         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23801_length_2988_cov_5.287078, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000325 DAOQNJ010000000
VERSION     DAOQNJ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23801_length_2988_cov_5.287078"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLG52_09380"
     CDS             complement(<1..387)
                     /locus_tag="PLG52_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41698.1"
                     /translation="MYSRLNASAKSLHNTLTANSEFLKLFAIPSVLFCLDFIFRASFG
                     LDLADAGADMALMAIATFSALVLDDKVMENKQTLVVCFFYALLFFVPWVICLKIISNN
                     PMMIFDVLDFRLVLSWLIGGIAFIFSG"
     gene            complement(580..1101)
                     /locus_tag="PLG52_09385"
     CDS             complement(580..1101)
                     /locus_tag="PLG52_09385"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB41699.1"
                     /translation="MDKEISELIQHFGFEALPVEGTLFVSTYRAGDMPNGKPYGTGMI
                     GMYCHEPRSVSYFHKLTIDEMWHFYGGDPLRLILLYPDGSTRDVVLGNEPLKGQLVQF
                     VIPAGVWQAGHCVEGGRYSLFGCTLAPGFTGDIFTGGTQTELLKLYPDRKKDILDYGL
                     AGGETEMPEGFAS"
     gene            complement(1116..1595)
                     /locus_tag="PLG52_09390"
     CDS             complement(1116..1595)
                     /locus_tag="PLG52_09390"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNB41700.1"
                     /translation="MKLPAHLHLDQHNIPYEAKSFSPETEKGAANVANVLGFPERQAV
                     KTLVFQVVDTGECVLVMLGGDQNAVSGNLKKAIGSRNIQMAPPERVKEVTGYIIGSIP
                     AFGWQPEGFRSFLEATLMDEPMLGTGAGQWGEEIMITPENLLKASGATVVNLTVKSG"
     gene            complement(1592..2080)
                     /locus_tag="PLG52_09395"
     CDS             complement(1592..2080)
                     /locus_tag="PLG52_09395"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNB41701.1"
                     /translation="MAQVIVVMGVSGSGKTTVGAKLAQELGWSFYDADDFHSEANREK
                     MAQGMALNDEDRAEWLASLRGLIRKHLEEGSSCVLACSALKQRYRDTLAVNEKVRFAY
                     LRGSFEQIETRMKRRKDHYMPVQLLQSQFEALEEPLDAVIVDISHTPEDIIHIIRKGI
                     DV"
     gene            complement(2092..>2988)
                     /locus_tag="PLG52_09400"
     CDS             complement(2092..>2988)
                     /locus_tag="PLG52_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB41702.1"
                     /translation="PIALIVATVAVSTSSIFIRFAQAGGAPSLGIAALRVTFATLMIA
                     PVALTRHMDEIKRFTRTEFLLGVASGIFLAAHFATWITSLEYTSVASSVVFVGTGPLW
                     VALLSPMLLKEHISRTTIVGLGLAVIGGIIIGLADACTWNNGLSCPALNDILKGRAMW
                     GNFLALAGAWAVTGYLIIGRKLRAETSLVPYIFLVYGMCAIALILFLFVTGNSPFGYD
                     AKTYGWIFLLAAIPQLIGHSTYNWALKYLTAAMVAVTVLGEPIGSATLAYFLLNETPS
                     LMTLVGGAFILIGIFLSSKGSS"
BASE COUNT          818 a          880 c          636 g          654 t
ORIGIN      
        1 tccagaaaaa ataaaagcga taccgcctat tagccaagat agtacaaggc gaaaatctaa
       61 aacatcaaat atcatcatag gattgttgga aataattttt aggcaaatga cccaaggtac
      121 aaagaataga agggcataaa aaaaacaaac cacaagtgtt tgcttgtttt ccattacttt
      181 gtcatctagt actaaagcgg agaaggttgc aattgccatt aatgccatgt ctgcgcctgc
      241 gtccgccaag tcaagaccaa atgatgcccg aaatataaaa tctaaacaaa ataaaacact
      301 tggaatagca aacaacttca gaaattctga attggcagta agggtattgt gaagtgattt
      361 tgcactagcg tttaatcgtg aatacattgc tcattgtgtt ttttatatct ttgacattat
      421 ttgcgggcta actatgtatt aacggaaccg ttcggctttt tacccccctg cggggtgtta
      481 taccgaaccg ttcggcataa tatcctttgt acggatgaat aaattatacg ccactccaat
      541 agaaaggggc aataaaaaca caaccttaca actcttccct cagctcgcaa acccttctgg
      601 catctctgtt tctccaccgg ctaacccata atccaaaata tccttcttgc ggtctgggta
      661 cagcttcaac aattccgttt gcgttccccc tgtaaaaata tcacccgtaa accccggcgc
      721 aagcgtacag ccaaatagcg aataccttcc gccctctaca caatgacccg cctgccacac
      781 cccagccgga atcacgaact gtaccaactg tcccttcaac ggttcattgc ccaagaccac
      841 atcgcgggtt gagccatcgg ggtaaagcag aatcaaacgc aacgggtcgc cgccataaaa
      901 atgccacatc tcatcgatcg tcaacttgtg aaagtaagac acactgcgcg gctcgtggca
      961 atacatcccg atcatccccg tcccgtacgg ttttccgttc ggcatatctc ccgcacggta
     1021 cgtactcaca aaaagcgtcc cttccaccgg caacgcctca aacccaaagt gttgaatcaa
     1081 ctcacttatt tctttatcca tcccgcctcc aaccatcaac cgctcttcac cgtcaaattc
     1141 accaccgtcg ccccgcttgc cttcaacaaa ttctctggcg tgatcatgat ctcctcgccc
     1201 cattgcccgg caccagttcc aagcatgggc tcatccatca gagtcgcttc gaggaaggaa
     1261 cggaatcctt cgggctgcca gccaaaggcg gggatcgaac cgatgatgta ccccgtcact
     1321 tccttcaccc gttccggcgg cgccatctga atattgcgcg aaccgatcgc cttcttcaaa
     1381 tttccagaga ccgcgttctg gtcaccgcct agcatcacca gcacacactc gcccgtatcc
     1441 accacttgaa agaccaaggt tttcaccgcc tgccgttcag ggaaccccaa aacattggcg
     1501 acatttgccg cacccttttc ggtttcgggc gaaaaacttt tcgcctcata cggaatattg
     1561 tgttgatcaa ggtgtaaatg agcagggagt ttcatacgtc tattcctttg cgaatgatat
     1621 ggatgatgtc ttcaggcgta tgagagatat caacgatgac cgcatctagc ggctcttcca
     1681 acgcctcgaa ctgactctgc aataattgta ccggcatgta gtgatccttg cggcgcttca
     1741 ttcttgtttc gatctgttca aacgacccgc gcaggtacgc aaaccggacc ttttcattca
     1801 cagccagggt atcgcgatag cgttgtttca acgccgagca agccagcaca caagaactgc
     1861 cttcttcaag atgcttgcgg atcaaccccc gcaacgatgc cagccattct gcgcggtctt
     1921 catcgttcaa cgccatgcct tgcgccatct tttctcgatt cgcttccgaa tgaaaatcat
     1981 cggcatcgta aaacgaccag cctaattcct gcgccagctt cgcgccaacc gtcgtcttcc
     2041 ccgagcctga aacgcccatc acaacgatca cctgcgccat aaaccctctc cttacgaaga
     2101 acctttgctc gaaagaaaaa taccgatcaa aatgaatgcc ccgccaacca gcgtcattaa
     2161 cgaaggggtt tcattcagca aaaaatacgc caacgtagca gacccgatcg gctcgcctaa
     2221 aacggtcaca gccaccatgg cagccgtgag atatttcaac gcccagttat aggtgctgtg
     2281 cccgatcaac tgcgggatcg ccgccaagag aaagatccag ccataggtct ttgcgtcata
     2341 cccaaaagga gagttgccgg ttacgaacag aaagaggatc aaggcgatgg cgcacatgcc
     2401 atacaccaaa aagatatacg gcaccagcga agtctctgcc cgcaattttc taccgatgat
     2461 gagatacccc gtcactgccc aggcgcccgc caaggcaagg aagttgcccc acatggcgcg
     2521 tcctttcaag atatcgttca acgccgggca ggagagacca ttattccatg tgcaggcatc
     2581 tgccagccca atgatgatcc cgccgatcac agccaacccc aagccgacga tcgtagtccg
     2641 cgagatatgc tctttcaaca gcatcggcga aagcagcgcc acccacagcg gacccgtccc
     2701 tacgaacacc accgagctgg caacactggt gtattccagc gaagtgatcc atgttgcaaa
     2761 gtgtgcggcg aggaaaatgc ccgaagccac gccgagcaga aactcggtcc gcgtgaagcg
     2821 tttgatctca tccatatggc gggtcagggc aaccggggcg atcatcaaag tggcaaaggt
     2881 cacgcgcaag gcggcgatcc ccaacgaagg cgctccgcct gcctgtgcaa agcgaataaa
     2941 aatgctggag gtagaaacag ccacggtagc aacgatcaaa gcaatcgg
//
LOCUS       DAOQNJ010000326         2986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23820_length_2986_cov_8.458547, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000326 DAOQNJ010000000
VERSION     DAOQNJ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23820_length_2986_cov_8.458547"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PLG52_09405"
     CDS             <1..1157
                     /locus_tag="PLG52_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNB41703.1"
                     /translation="ALAVKLIEDPNIFLSAVQIGITLIGVLSGAVGGATIAEKLAVFF
                     EKIPYIGPYSESVSLGLVVLVITVLTIWLGELVPKRLGINSPERIAQIVAGPMLFISR
                     VFSPLIKLMSNATNFVLRIFGVNPSVEPPITEEELQLLIDQGTQAGVFEEAEQDMVEG
                     IFSLGDTRVYSVMTPRTEIVWLDVSDSTEEILEKMSGSPYSRFPVRQDSLETIVGIVK
                     SRDLLVSILSQKQINLKELAKPAHFIPETMLASRALEVLKKNNAEMLLVVDEFGGVQG
                     LLTINDILEEIVGVMEGDEPQATQRQDGSWLLDGMLEVDEFKEIFNLNELPHETEYET
                     LSGFIMTSLGRLPQTADHFEWNNLRFEVVDMDGRRVDKVLVTAKQKTGTD"
     gene            1221..2384
                     /locus_tag="PLG52_09410"
     CDS             1221..2384
                     /locus_tag="PLG52_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB41704.1"
                     /translation="MSPESSPSKTKVCPTCGTRLSENAVRCLVCGTEFGAQPAPKAVK
                     KTEKSVQAASMPQLTLSLPAAIGSLLAVIAVAAGLTFFLVPKDPETFSNVETSTPTET
                     PTATIPATATLPATETPTPTLQPPFEYTVSAADGSCSQIAFNFNVSVQSIIITNNLPS
                     SCPISVGQKLMIPYPTPTVPPPATNTPPPDVATQQACETVQITVQENDTLSSIAANYA
                     VSMDAIKEFNGLTTDNVFLGQVVQIPLCERAPDPNKPTPTATLPPPYPAPNLLLPADG
                     AAFTLANDVVTLQWASVGVLRDGEAYQVVVEDVTSNQTRRITDYVTDTKYIVPSSFRP
                     KDNVAHVMRWSVVPVLQSGVDDQGKPIWIASGATSERRVFTWVGVAVQGTPNP"
     gene            2475..2660
                     /locus_tag="PLG52_09415"
     CDS             2475..2660
                     /locus_tag="PLG52_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41705.1"
                     /translation="MLNTIVKIFAVLLIASGIIWILQGVNILPGSFMTGDPQWAINGA
                     ITAAIGAGLFWFVSRKK"
     gene            2660..2878
                     /locus_tag="PLG52_09420"
     CDS             2660..2878
                     /locus_tag="PLG52_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41706.1"
                     /translation="MPDTIQVGDRVQIRLDEKFGEREGWYGGTVVRIDPYSEHRSFYW
                     VRLDSEAQAILRVAEISVFNPKNIKKVS"
BASE COUNT          780 a          784 c          698 g          724 t
ORIGIN      
        1 cggcattggc tgttaagtta atagaagacc ccaatatatt tttatcggca gtgcaaattg
       61 gcatcacctt gatcggcgtg ctttctggtg cggtcggtgg tgcgaccatt gctgagaagt
      121 tggctgtctt ctttgagaaa atcccatata tcggacccta tagtgaatcc gtttcattag
      181 ggcttgtggt acttgttatc accgtgctca ccatttggtt gggcgaactc gttccgaagc
      241 gacttggcat taacagccca gaacggatcg ctcagatcgt ggctggaccg atgctcttca
      301 tttcgcgggt gttctcacca ttgatcaaat taatgagcaa tgctacgaat tttgtcttgc
      361 ggatatttgg cgtaaatcct tcggtcgaac cacccattac agaagaagaa cttcaattgc
      421 taattgacca aggcacacaa gccggcgttt ttgaagaagc ggaacaagat atggtcgaag
      481 gcattttcag ccttggcgat acccgcgtct attcggtgat gactccacgt actgaaatcg
      541 tttggctgga tgtaagcgac tcaacggaag agattctcga aaagatgagc ggcagtccat
      601 actcacgctt tccggtacgc caagactcat tagagacgat tgttggcatt gttaaatcac
      661 gagatttgtt ggtgtccatt ctttctcaaa agcagatcaa cctgaaagaa cttgccaagc
      721 ctgcgcactt catccctgaa accatgttgg cttcacgtgc cttagaggtg ctaaaaaaga
      781 ataacgctga aatgttactc gtcgtggatg aatttggcgg cgtacaaggt ttgctgacga
      841 tcaatgacat cctcgaagaa attgtcggcg tgatggaagg tgacgaacca caagccacac
      901 aacgtcaaga tggttcgtgg ttattagatg gcatgcttga agtggacgaa ttcaaagaga
      961 ttttcaatct aaacgaatta ccgcacgaaa ccgagtatga aaccctgagc ggattcatta
     1021 tgacttcgct cggtcggctg ccacaaaccg cagaccactt cgagtggaac aacctccgct
     1081 ttgaagtggt agatatggat gggcgccgtg tcgataaagt tttagtcacg gcaaagcaaa
     1141 aaacaggcac agactaagcc cgttcgtagc taaatcgcct aaaaatcgta gatttgttcg
     1201 atggtataat tttcgcccgt atgagtcctg aatcatctcc cagcaaaaca aaggtctgtc
     1261 ctacctgtgg aacacgccta agtgagaatg ccgtccgctg tttggtatgc ggaacggaat
     1321 ttggcgcaca gcctgcaccc aaagcagtga agaaaacgga aaaatccgtt caagctgcca
     1381 gcatgccaca gcttacgctc agccttcccg cagccattgg ctcacttctg gctgtgatcg
     1441 cagttgcagc aggtcttaca ttcttcctcg tccctaaaga cccggaaact ttttccaatg
     1501 tagagacctc cacacccaca gaaaccccca ccgcaaccat ccctgcaacc gcaaccttac
     1561 ctgctacaga aacacccacg cccactctgc aacctccctt tgaatacacg gtttcggcag
     1621 cagatggttc ttgttcacag atcgcgttca acttcaacgt atctgtacaa agcattatca
     1681 ttaccaacaa cttaccttcc agttgtccaa tttcggtggg gcaaaaattg atgattccgt
     1741 accccacccc aacggttccg cccccagcca ccaacacacc gccgccagat gttgcaaccc
     1801 aacaagcctg cgaaaccgta caaatcaccg tccaagaaaa cgatactctt tctagcatcg
     1861 ctgcaaacta tgccgtttcc atggatgcca tcaaagagtt caacggtcta acaaccgata
     1921 acgtcttcct tgggcaagtg gttcagattc cgctctgcga acgcgcaccg gatcccaata
     1981 aacctacccc cactgcgacg cttccgcctc cttaccccgc cccgaacctg ctcctgcctg
     2041 cggacggcgc tgccttcacc ctggcaaacg atgttgtcac cttgcaatgg gcatctgtcg
     2101 gtgttctgcg tgacggagaa gcctatcagg ttgtggtcga agatgtaacc tccaatcaga
     2161 ctcgccgcat cacagactat gtgaccgaca caaaatacat cgtgccgtcc agcttccgcc
     2221 cgaaagacaa tgtagcgcac gttatgcgct ggtcagttgt gcccgtcctg caatcaggcg
     2281 tggatgacca aggcaaaccg atctggatcg cctccggcgc aaccagcgaa agacgtgtct
     2341 tcacttgggt cggagtcgca gttcagggaa cgccgaatcc gtaacaggtc cagtacgaag
     2401 cgaaaatcat taagtcaact ccctgtccgc ttgggcaggg agttttataa ataaatccat
     2461 attaggaaaa aatcatgctc aacactattg taaaaatctt tgctgtactc ttaatcgctt
     2521 ctggcattat ttggattctg caaggcgtca acattttgcc cggcagcttc atgaccggtg
     2581 acccacaatg ggcgatcaat ggagccatta cagccgccat tggagccggg ttattctggt
     2641 tcgtctctcg caaaaagtaa tgccagatac cattcaggtc ggtgaccggg ttcagattcg
     2701 cctcgacgaa aagtttggcg agcgagaagg ctggtacggc ggcacggtcg tccgcattga
     2761 cccgtattct gagcatcgca gtttttattg ggtgcggctc gactctgaag cgcaagccat
     2821 cttaagagtt gccgaaatct cggtctttaa tcccaagaat atcaagaagg tttcatagca
     2881 gtcgtaagag ggggcatctg tattgccgtc ttaatcaagc cggaaggagc tttagatgag
     2941 tagaaaaaag aacgcaatgg catatgggtt tacagcattg ctcgtg
//
LOCUS       DAOQNJ010000327         2986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23821_length_2986_cov_7.699761, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000327 DAOQNJ010000000
VERSION     DAOQNJ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23821_length_2986_cov_7.699761"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PLG52_09425"
     CDS             <1..842
                     /locus_tag="PLG52_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNB41707.1"
                     /translation="YYDGSLRQINMNAYNEYVNTARKKREDSFASNPQSWLNLVGLFW
                     LEEGENSFGRSPEAKISLPQFPLDFCGHFLFKNGLVTVHPQTAMTMNNGVIESRPLYT
                     DKDPQADTLEVGTLIMKIIVRGDATLVRVWDKEAELKKGFKRFNYYEPNPKFKVTAKY
                     VRYDPPKPTIRIEGIGTEVQTFFMGEAQFTLNGKDYSLQAEESGDELLFNFKDETNTD
                     TTYGGGRRFYLLPPEGDELILDFNLTDNWPCAYTPFATCPIPPKENTLKVRIEAGEKK
                     FKK"
     gene            complement(876..1436)
                     /locus_tag="PLG52_09430"
     CDS             complement(876..1436)
                     /locus_tag="PLG52_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNB41708.1"
                     /translation="MELFEAIHGRTTVSKVKQDVISREIIEKLLSAGAQAPNHHKVRP
                     WRFVVLTGDGRKKLGDVMTASFADRNPGAPAEGLDKTRNLPLRAPVVIAVGADKPADA
                     KIQEIENISAASAACQNILLAAHALGLGAIWRTGEWARDVKVKEFLGFAPDQHIVGFI
                     YIGNPDIMPDPYTRIGFEDRVTWIDK"
     gene            1484..2524
                     /locus_tag="PLG52_09435"
     CDS             1484..2524
                     /locus_tag="PLG52_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNB41709.1"
                     /translation="MKIFYSEEHRNHYPPFEVFDGGTRVQYFENPDRMDQILNALQGE
                     NWVTFLEPDDFGLDPILAVHDRDYLNFLASCWDEWLASDPEVAASPETHAFLPATFAL
                     RRKNARPTSSIRGRGGYYIMDLSASIVAGTYKAALTSANIALSATSAVYGGDKSAFAL
                     CRPPGHHAGKDYAGGYCFINNAAVAANWLANKGKVAILDVDYHAGNGTQDIFYDRNDV
                     LSISIHGDPDFEYPHYVGFAEETGTGAGLGFHRNFPLPKGTKDEEYLSALDEALNLIR
                     DYAPQYLVVSFGADAFDGDPLGLFKLTRNGFSEIGKRIAGLNLPTAIIMEGGYANAAL
                     GDNTKTLLENFK"
     gene            2521..>2986
                     /locus_tag="PLG52_09440"
     CDS             2521..>2986
                     /locus_tag="PLG52_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41710.1"
                     /translation="MKKWHSLLFVMVFTLTACGAAATEPPPPTETSTPIIVYITATSQ
                     PTLTPTITFTPGPTNTVEPSITPTPEFTFPTVTVNKQAHCRYGPSAAFLHAADLYAGD
                     TGTVRNRAMYSNWLYIKFDKLNYFCWVAPSVVDVVGDVTTLKRYEPNLQSIGS"
BASE COUNT          813 a          749 c          668 g          756 t
ORIGIN      
        1 gatattatga tggaagtctt aggcagataa acatgaatgc atataacgaa tatgtcaaca
       61 ccgctcgcaa aaagcgggaa gatagttttg cgtcgaatcc acagagttgg ttaaaccttg
      121 tcggcttgtt ttggctcgaa gaaggcgaaa acagcttcgg gcgtagtcct gaggcgaaga
      181 tttcccttcc acaattccct ctagattttt gtggtcactt cctcttcaaa aacggactcg
      241 tcacagtaca tccgcaaaca gcgatgacca tgaacaatgg cgtgatagag tctcgccctt
      301 tatatacaga caaagaccca caagccgata cgcttgaagt ggggacactc attatgaaaa
      361 ttattgtgcg tggagatgcg actcttgtac gcgtatggga caaggaagca gaactcaaga
      421 aaggcttcaa acgctttaac tactacgagc ccaacccaaa gtttaaggta acagccaagt
      481 atgttcgcta cgacccgccc aagccaacga ttcgaataga aggcattggt accgaagtgc
      541 aaactttctt tatgggagaa gcccaattca ccttgaacgg aaaagactat tcacttcaag
      601 ccgaagaaag tggcgacgaa ttgctcttta atttcaagga cgaaaccaat acagatacga
      661 cttatggtgg tggacgcagg ttttatcttc tgccgccaga aggcgatgaa ctcattttag
      721 atttcaactt aactgacaat tggccctgcg cgtacacacc ttttgccacc tgccctattc
      781 cgcccaaaga aaacacattg aaggtccgca tagaagctgg cgagaagaaa tttaagaagt
      841 aaacaaaaag cgagtggcaa gccactcgct ttttgttatt tatcaatcca tgttacacga
      901 tcttcaaacc caattctagt atatgggtct ggcattatgt ctgggttccc aatataaata
      961 aaaccaacaa tatgctgatc gggcgcaaag ccaaggaatt cttttacttt cacatcgcgt
     1021 gcccattcgc cggtgcgcca aatagcgccc aagcctaaag catgtgccgc taacaaaata
     1081 ttctggcaag ctgctgaagc cgcagaaatg ttttcaattt cttggatctt tgcatcagca
     1141 ggtttatctg cgccaacggc aattacaacc ggtgcgcgga gcggcaagtt tcgggtttta
     1201 tccaagcctt cggctggcgc acccgggttc cggtcggcga aagaggcggt cattacatct
     1261 cctaacttct tacgcccatc cccggttaac acaacaaaac gccagggtct cactttgtga
     1321 tgattcggcg cttgtgcgcc ggcacttaat aatttttcga tgatctcgcg tgaaattaca
     1381 tcttgcttca ctttgctgac cgttgttcgt ccgtgaattg cttcaaaaag ttccatttct
     1441 atacctccgg gagtgagacg cttgcaggta aaattgtctt accatgaaaa tattttactc
     1501 agaagaacac cgcaatcatt atccaccttt tgaagtgttc gatggcggca caagggttca
     1561 gtattttgaa aacccagacc gtatggatca aatcttaaat gcgctacagg gtgaaaactg
     1621 ggtgacattt ttagagccag atgattttgg actcgacccg atccttgctg tgcacgaccg
     1681 tgactacctc aacttcctcg cctcctgctg ggatgagtgg ctggcatctg accccgaagt
     1741 tgctgcttcg ccagaaacgc atgctttctt gcccgctacc tttgctttgc gaagaaaaaa
     1801 cgcacgccct acctcctcca ttcgtggacg cggcgggtat tacattatgg acctttctgc
     1861 ttccattgta gcagggacgt ataaagccgc gctgacttca gcaaatattg ccttgagcgc
     1921 cacctcagca gtttatggcg gagataagtc ggcttttgcg ctttgccgtc cgccaggcca
     1981 ccatgcaggc aaagattacg cgggcggata ctgctttatc aacaatgcag cggtggctgc
     2041 aaactggctg gcaaataaag gcaaagttgc catattagat gttgactatc atgcagggaa
     2101 cggcacccaa gatattttct atgaccgcaa tgacgttctc agcatctcga ttcatggcga
     2161 cccagatttt gaatatccgc actatgtagg tttcgctgaa gaaacaggga ctggagccgg
     2221 gcttgggttt caccgaaact ttcctttacc caaaggcaca aaagatgaag agtatttatc
     2281 ggctttggac gaagcattga acctgattcg agactatgcg ccacaatatc ttgtcgtctc
     2341 tttcggcgca gatgcctttg atggtgaccc actggggttg ttcaaactga ccagaaatgg
     2401 ttttagcgag atcggcaagc gaattgctgg attaaacctg cctactgcga ttattatgga
     2461 aggcggttac gccaacgcag cgctcggaga caacaccaaa actttattgg agaacttcaa
     2521 atgaaaaaat ggcactcgct tttattcgtc atggtcttta ccctaacagc ttgcggcgcg
     2581 gctgcaacag aacctccccc gccgacagaa acctctacac caatcatcgt ctatattaca
     2641 gccacgtcac aaccgacatt gacaccaacg attacattca cacctggtcc gaccaacacc
     2701 gttgaaccaa gcatcacccc tacgcctgaa ttcacatttc ccacagttac ggttaacaag
     2761 caagcacatt gccgctatgg tccctctgca gcatttctcc acgccgcaga cttatatgct
     2821 ggggatactg ggaccgtacg aaaccgagca atgtatagta attggcttta catcaagttc
     2881 gacaagctca attatttctg ttgggtggca ccatctgttg tggatgtagt tggagatgtc
     2941 actacactaa agcgctacga gccgaatctt caatccattg gcagca
//
LOCUS       DAOQNJ010000328         2982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23873_length_2982_cov_7.509737, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000328 DAOQNJ010000000
VERSION     DAOQNJ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23873_length_2982_cov_7.509737"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLG52_09445"
     CDS             complement(<1..214)
                     /locus_tag="PLG52_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41711.1"
                     /translation="MPKSTKTDLKIRALMAVRDYGVNLTHFRNAMNEWAGLNATDMEC
                     LRLLSQKGTATPTELAKHTGLTSGATT"
     gene            294..743
                     /locus_tag="PLG52_09450"
     CDS             294..743
                     /locus_tag="PLG52_09450"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB41712.1"
                     /translation="MSPSKETKKTTAKSTGLSAEERAAMKETLKERKMAANKEEAEKA
                     ALAAIAAMKEPDRSMAKRIHTIIKEHAPTLSAKTWYGMPAYVDKDEKVVCFFQTKFKF
                     NYKYATLGFQDAAHLDDGAMWPVAFALKELNSAEEKQVIALIKKAVS"
     gene            752..1282
                     /locus_tag="PLG52_09455"
     CDS             752..1282
                     /locus_tag="PLG52_09455"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNB41713.1"
                     /translation="MATICFETVPQKVGDWTILVLPKKESAKLPSRGMTLVEGTINGF
                     HSKIVLEPDGKGSHWFKVDARLSKAAGIEKGGMVTMALEPSKEWPEPEVPSDLKKALS
                     SDSAAKALWAEITPMARWDWLRWIRSTNNQETRKRRIEVALSKLKSDKRRPCCFNRNE
                     CTEPAVSKNGVLIEPI"
     gene            1416..>2982
                     /locus_tag="PLG52_09460"
     CDS             1416..>2982
                     /locus_tag="PLG52_09460"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41714.1"
                     /translation="MPQEYIEIHGARENNLKNVSLRIPKRKITIFTGVSGSGKSSIVF
                     DTIATESQRLLNENFSMFVRNFLPKYSQPEADSIENLSMSIVVDQKRMGGGSHSTVGT
                     ITDINTILRMMFSRLGQPHVGPTNVFGFNDPQGMCPNCNGLGRKLDVDMEKFLDKSKS
                     LNEGAILFHEYAVDSWYWSNLINTGLFDPDKKISKYSQKEMHDLLYSEPIKVKTKVGA
                     KDFNMTYEGIITRFTNKYIKQDLKTKSERTQKSIEPYLTMGPCSECNGSRLNKTILNC
                     KINGYNIADLTSMEIPDLIEAVKKIKIPEAQPMVKALLERLQHLVDIGLEYLSLSRET
                     DTLSGGESQRVKIVKHLGSSLTDVIYIFDEPSVGLHPRDVHRMNELLQKLRDKGNTVI
                     VVEHDPDVIKVADHIVDVGPHAGPQGGEIVFEGSYVNLLKAKTLTGTHMQQSVPIKDS
                     FRAPTGKLPIKNAKVNNLQNVSVNIPTGVLTVVTGVAGSGKSSLINEVFQYQHPDAVV
                     IDQSAVGVNSRSNP"
BASE COUNT          844 a          714 c          751 g          673 t
ORIGIN      
        1 cagtggtggc gcccgaagtg agccccgtat gtttggcaag ttcggtggga gtggcagtgc
       61 ctttttgcga tagcagcctc aggcattcca tgtcggtggc gttgagtccc gcccattcgt
      121 tcatggcatt gcggaagtgg gtaaggttca ccccgtaatc tctcaccgcc atcagggcgc
      181 gtatttttag atcagtcttt gttgattttg gcatggaatg tcccttgaca ttatctttat
      241 gataaagtat ctttattgta taactaaatt cgcaaacacg caaggagatt tctatgagcc
      301 ctagcaaaga aacaaaaaag accactgcga agtccacggg tctttcagcg gaagaaagag
      361 ccgcgatgaa ggagactctc aaagagcgga agatggctgc aaacaaggaa gaggcagaga
      421 aagccgcact tgccgcaatc gccgcgatga aggaaccgga tcgctcgatg gcaaaacgca
      481 tccacacgat catcaaggaa catgcaccga ccctctcagc gaaaacctgg tatgggatgc
      541 ctgcctatgt ggacaaagat gaaaaagttg tctgcttttt ccaaaccaag ttcaagttta
      601 actataaata cgccacgctt ggctttcaag atgcggcaca ccttgacgat ggcgcaatgt
      661 ggcccgttgc ctttgcgttg aaagaattaa actctgctga agagaagcaa gtcatcgcgc
      721 taataaagaa agctgtgagt taaagaggat catggcaacc atctgttttg aaaccgtgcc
      781 gcaaaaagtc ggcgactgga ccattcttgt gctacccaaa aaagagagcg caaaacttcc
      841 atcgcgcggc atgacattgg ttgaagggac gatcaatggc ttccattcca agatcgtcct
      901 tgaaccagat ggcaaaggaa gccattggtt taaagtggat gctcgtttga gtaaagccgc
      961 agggatagaa aaaggcggaa tggtaacaat ggcacttgaa ccgtccaaag agtggcccga
     1021 gcctgaagtg ccttccgact tgaaaaaggc tctatcgtct gactcggcag caaaagcctt
     1081 gtgggcggaa attacaccga tggcgcgctg ggactggcta cgttggatcc gctcgaccaa
     1141 caatcaggaa acacgcaagc ggcggattga ggtagcctta tcgaaactca aatccgacaa
     1201 gcgcagaccg tgttgtttca atcgtaacga atgcaccgaa cctgcggttt cgaagaatgg
     1261 cgtgctgatc gaaccaatat agacaacaat tttcattttg tcttgacttg tcagaataaa
     1321 tgttctaaaa ttctgatact ttcgggcggg caaaagcgga tttgcttttg cccgcccatt
     1381 ttgttttacc tagcagagat tattgaggag agacaatgcc gcaggaatat atcgagattc
     1441 atggcgcacg tgagaacaat ttaaagaacg tttcactgcg catccccaaa cgcaagatca
     1501 ccattttcac aggcgtgtcc ggttcaggta aatcgtccat cgtgttcgac acgatcgcga
     1561 ctgaatcgca gcgattgctt aacgaaaact tcagcatgtt cgtgcggaac ttcctgccga
     1621 agtattccca accagaagcg gactccatcg aaaacttgag catgtccatt gtggtggatc
     1681 agaagcggat gggcggcggc tcacactcga ccgtgggcac gatcaccgac atcaacacga
     1741 tcctacgcat gatgttttcc cgcctcggtc aaccccatgt gggacccacc aacgtcttcg
     1801 gctttaacga cccgcaaggg atgtgcccga actgcaacgg acttgggcgc aaactcgatg
     1861 tggatatgga gaagttcctc gataaatcca aatccctgaa cgaaggcgca atcttattcc
     1921 acgaatatgc tgtggatagc tggtactgga gtaacttaat taatacaggt ttgttcgacc
     1981 ccgacaagaa aatttccaaa tattctcaga aagaaatgca cgacctgtta tatagcgaac
     2041 ccatcaaggt caagaccaag gttggcgcaa aagacttcaa catgacctat gaaggcatca
     2101 tcactcgctt taccaacaag tacatcaaac aggatctaaa aacaaaatct gaacgaaccc
     2161 agaagtcgat tgaaccctat ctgacgatgg gaccgtgttc tgaatgtaac ggctctcgtt
     2221 tgaacaagac gatcctgaac tgcaaaatta acggctacaa cattgccgac ctgacgagta
     2281 tggaaatccc tgatttgatt gaagccgtga aaaagatcaa aattcctgaa gctcagccga
     2341 tggtcaaggc attgcttgaa cggttacaac atctcgtgga tattggcttg gaatacctca
     2401 gcctcagccg cgaaacggac acactttcgg gcggcgagtc acaacgtgta aagatcgtca
     2461 aacacctcgg cagcagcctc accgatgtga tctacatctt cgatgagccg agcgtgggtt
     2521 tacatccgcg cgatgtgcat cgtatgaacg agctgttgca gaaactgcgt gacaagggca
     2581 acaccgtcat tgtggtggaa catgaccctg acgtgatcaa agtggctgac catatcgtgg
     2641 acgtgggtcc gcatgcaggt ccgcagggag gcgagatcgt tttcgaggga agttatgtca
     2701 atttgctcaa agctaagacg ttaacgggta cgcatatgca gcaatccgtc ccaatcaaag
     2761 attcgttccg cgcgccgacg ggaaaattgc cgatcaagaa tgcaaaggtc aacaatctgc
     2821 aaaacgtcag tgtgaatatt ccaactggcg tattgacggt ggtgacgggc gtggcagggt
     2881 ctggtaagag ttcacttatc aacgaagtgt ttcagtatca acatcctgac gcggttgtga
     2941 tcgatcaatc tgcggtggga gtcaacagcc gctcgaaccc cg
//
LOCUS       DAOQNJ010000329         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_23892_length_2981_cov_11.032126, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000329 DAOQNJ010000000
VERSION     DAOQNJ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23892_length_2981_cov_11.032126"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PLG52_09465"
     CDS             <1..1108
                     /locus_tag="PLG52_09465"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41715.1"
                     /translation="HTRPIITEYSGKVRFENVEEGVTVAKQIDEVTGLSTLVVIDPKR
                     RGVLQSKGLRPLVKFLDDDGNEIKMVGSNQSVSITFQIGCIITVRDGQQVSVGEVLAR
                     IPQETSKTRDITGGLPRVAELFEARSPKDAGMLAEVTGIVSFGKDTKGKQRLVITDLE
                     GIVHEYLIPKDKHVMAHDGQVVNKGEIIVDGPADPRDILRLQGVEALARYITDEVQDV
                     YRLQGVRINDKHIEVIVRQMLRRVQVINAGDSSFIPGEQVERADLLIENERLIAEKKM
                     PATYEFLLLGITKASLSTDSFISAASFQETTRVLTEAAIMGKKDDLRGLKENVIVGRL
                     VPAGTGLSFHSKRRNQEKIFESGLESDISIDSTV"
     gene            1347..>2981
                     /locus_tag="PLG52_09470"
     CDS             1347..>2981
                     /locus_tag="PLG52_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNB41716.1"
                     /translation="MKGKLWPKIVAAMLGGLLIGAAHANIPSVPNELYEALKLDREKV
                     TPKELHEALVKRYKDPAQGSGRGTLAQYWEPIPYGIYLDPNTFYKSPTTNKEVASRKE
                     CVECHTDESPVWVQAWKRSSHANLDKVRNLKPGDPTFYKKAKLEDIEKNLRSMGRLAE
                     GENLKEVGCIDCHVDIGAKKKADHTKDIRMPTADVCGTCHLQEFAERESERDTMIWPH
                     DQWPDGRPSHALDYKANVETTVWAAMPQREVAEGCSMCHTNQNKCDSCHTRHEFSAAE
                     SRRPEACATCHSGVDHNNWEAYSMSKHGKIVGMLGNQWNWEAPLKDAYAVGGQSAPTC
                     AGCHMEYEGEYSHNMVRKIRWANYPFVPGIAENIKSEWSEKRLDSWVVTCTQCHSERF
                     ARSYLDLMDKGTLEGLAKYQEANAIVHQLYKEGLLTGQKTNRPAPPAPEKEGYAYFAQ
                     LFWSKGNSPAAIELKVLEMHENDLAKMHVGLAHVNPGGWTYTEGWGPINRAYVEIQDE
                     NTRIREMVALQERVKNLETKKTSLLDLDGTAEKISLG"
BASE COUNT          925 a          649 c          779 g          628 t
ORIGIN      
        1 gcatacgcga ccgatcatta ctgagtatag cggtaaggtt cgtttcgaga atgtcgaaga
       61 aggtgtgacg gttgccaaac aaatcgatga agtcactgga ttatcgacat tggtagttat
      121 tgatccgaag cgccgtggtg tattgcaatc aaaaggtttg cgtcctttgg tgaaattctt
      181 ggatgacgac ggtaatgaaa ttaaaatggt tggaagtaat cagtccgtga gtattacatt
      241 ccagattggt tgcatcatta ctgtacgtga cggacagcaa gtaagtgttg gtgaggtatt
      301 agcacgaatt ccgcaagaaa catccaaaac cagggatatt actggcggtt tgccgcgcgt
      361 ggctgaattg tttgaggcgc gatcaccgaa agatgctggt atgctagctg aagtaaccgg
      421 tatcgtgtcg ttcgggaaag ataccaaagg taagcagcgg cttgtcatca ctgatctgga
      481 aggtatcgtt catgaatatc tcataccgaa ggataaacat gtcatggcgc atgatgggca
      541 agttgtcaac aaaggtgaaa ttatcgttga cggtccagct gatccgcgcg acatattgcg
      601 tttacaaggt gttgaagcac tagctcgcta tattaccgat gaagttcaag atgtataccg
      661 tttacaaggt gttagaataa acgacaaaca cattgaagtc atcgtgcgtc agatgttaag
      721 gcgagtgcag gttattaatg caggagattc gtcatttata cctggcgaac aagttgagcg
      781 agctgatttg ttaattgaaa atgagcggct aattgctgag aaaaaaatgc cagctaccta
      841 tgagttctta ttgctcggta ttacaaaagc atcgctatca acagattctt tcatctcagc
      901 tgcgtcattc caggaaacaa cgcgagtatt gactgaggca gccatcatgg gtaaaaaaga
      961 tgatttgcga ggactgaaag agaatgttat tgttggtagg cttgttccag cagggacagg
     1021 attatctttt catagcaaac gaaggaatca agaaaagata ttcgaatccg gtttggaatc
     1081 agatatttca atagatagta cagtctagca attaaaagcc tattactcga ttatcaagtc
     1141 atcccttgac agttataaga gtactaagta gtctttactg ttgtgtggct attactgagg
     1201 ctatggcgga tgatcagagg ctgaggtgtg ataaaaaaga gtaagggcaa ttagccgaac
     1261 tctgctgagg ttgcacgaga gatcaataag aagagagaat tttgagggat aggaaggaag
     1321 aaaatggaaa accaaaggag agagagatga aaggcaagct atggccgaag atagtagcgg
     1381 caatgttagg tggtctgctg ataggagcgg cacacgcgaa cataccgagc gtaccgaatg
     1441 aattatatga agcattgaag ctggatcgcg agaaagtaac gccgaaggaa ctgcacgaag
     1501 cgctggttaa gcgctacaag gatcctgcac aaggatcggg acgcggcacc ctggcgcaat
     1561 actgggagcc gataccgtac ggtatatatt tggatcccaa tacattttac aaatcaccga
     1621 ccacgaataa agaagttgca agccgtaaag aatgcgtgga atgccacacc gacgaatcac
     1681 cggtatgggt acaagcatgg aagagaagca gccatgcgaa cctggacaaa gttcgcaacc
     1741 tgaaaccggg cgaccctact ttttacaaga aagctaagct ggaagacatc gaaaagaact
     1801 tacgctcgat gggcagactg gccgaaggcg aaaacctgaa agaagtgggc tgtatagact
     1861 gccacgtaga cattggcgcg aagaaaaaag ccgatcacac caaagacatc agaatgccga
     1921 cagcggacgt ttgcggcacc tgtcacctgc aagaatttgc agaacgtgaa tcggaacgcg
     1981 acacgatgat ctggccgcac gaccaatggc cggatggacg cccatcgcac gcactggact
     2041 acaaagccaa cgtagaaacc accgtatggg cagcgatgcc gcaacgcgaa gtagccgaag
     2101 gctgctcgat gtgccacacc aaccaaaaca aatgtgactc ctgccacacc cgtcacgaat
     2161 tctcagcagc ggaatcccgt agaccggaag cttgcgcaac ctgtcacagc ggcgtagacc
     2221 acaacaactg ggaagcgtac tccatgtcca aacacggcaa gatcgttggc atgctgggta
     2281 accaatggaa ctgggaagca ccgttaaaag acgcctatgc agtaggcgga caaagcgcgc
     2341 caacctgtgc cggctgccac atggaatacg aaggcgaata cagccacaac atggttagaa
     2401 agattcgctg ggcgaactac ccgtttgttc ctggaattgc agaaaacatc aagagcgaat
     2461 ggtcggaaaa acgtctggac tcatgggttg ttacatgtac ccaatgccac tcggaacgtt
     2521 ttgcccgctc ctatctggac ctgatggaca aaggcacact ggaaggactg gctaaatacc
     2581 aagaagccaa tgccatcgta caccaactgt acaaggaagg cctgttgaca ggacaaaaaa
     2641 ccaaccgtcc tgcaccgcct gcaccggaaa aagaaggcta tgcctacttt gcccaactgt
     2701 tctggtcgaa aggcaacagc cctgcagcga tcgaactgaa agtgctggaa atgcatgaaa
     2761 acgacctggc caaaatgcac gtaggcttgg cgcacgttaa cccgggcggc tggacctaca
     2821 ccgaaggctg gggtccgatc aaccgcgctt atgttgaaat tcaagacgaa aacacccgca
     2881 tccgtgaaat ggttgcactg caagaacgtg ttaaaaatct tgaaaccaag aaaaccagtc
     2941 tactcgactt agacggcaca gcagagaaaa tctctctggg c
//
LOCUS       DAOQNJ010000330         2972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24001_length_2972_cov_9.135413, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000330 DAOQNJ010000000
VERSION     DAOQNJ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24001_length_2972_cov_9.135413"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLG52_09475"
     CDS             <1..387
                     /locus_tag="PLG52_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41717.1"
                     /translation="HAVILLGDEYINFTLLQVFIPFSTVDYRPFWVGIGQLGFYIWLI
                     VAMSFYVRPMIGQKFWRVLHYLSFAMYFVGIFHAIFSGTDSSMSWAQNYYWFSAGSLL
                     FLFFVRIVSIVIDGLFPVKKPVPVQR"
     gene            complement(474..1052)
                     /gene="ruvA"
                     /locus_tag="PLG52_09480"
     CDS             complement(474..1052)
                     /gene="ruvA"
                     /locus_tag="PLG52_09480"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNB41718.1"
                     /translation="MIATLRGEVSHVEDNAIIVEVGGVGMRVFVPAPMRTKASAGEMI
                     FLFTHLVVREDALTLYGFESQSDRDLFNILLGVDGVGPKVALSVLSTLTLETIQRAIF
                     ADEEELLSKVPGVGKKTAQKMAIHLKDKLKPTDALSKMAALADYDSEVLAALAALGYS
                     VVEAQAALQALPKDAPKDVEERLRVALQYIGK"
     gene            complement(1110..1592)
                     /gene="ruvC"
                     /locus_tag="PLG52_09485"
     CDS             complement(1110..1592)
                     /gene="ruvC"
                     /locus_tag="PLG52_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNB41719.1"
                     /translation="MTLALGIDPGTATTGYGFVRLMPDGELVAVDYGVILTPKEATAS
                     ARLVMLFDDLNKLLKKHKPETAAVEKLFFSKNVTTGIAVGQARGVILLALEKAGISTY
                     EYTPNEVKQAVAGYGSAEKRQVQEMVRALLQLEKIPKPDDAADALAIAITHLNTARYD
                     "
     gene            complement(1646..2398)
                     /locus_tag="PLG52_09490"
     CDS             complement(1646..2398)
                     /locus_tag="PLG52_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNB41720.1"
                     /translation="MSGHSKWSTIKRKKGVADAKRGAIFTRLAREITIAAREGGDPDT
                     NFRLRLAVEKARAENMPKDNIERAIKKGTGEDKDAAVFEELTLEGYGPHGSAVMVSCV
                     TDNRVRTVSDVRHIFSKFGGNMAEAGAVGWQFDRKSYFSFPSSAMAFDKAFELGVEAG
                     ADDVVDSGEEIEIFGPVEAFKTIMDTLGKAKVQPAEAGLRLIAKQEIDLGVEEIMAVM
                     KVVENLEDLDDVQDVYHNVKVSDEALAALENA"
     gene            complement(2579..>2972)
                     /locus_tag="PLG52_09495"
     CDS             complement(2579..>2972)
                     /locus_tag="PLG52_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41721.1"
                     /translation="LGAGSGAISGTWNGITTAAIMGLVTGAINGALTAALVIRTAGIT
                     GGVSTGAYTGMAFGAFLGLIFGIFLPESFRQSVLKLDVLILNVIFAGRFEAAILFSFL
                     FSVLSTAVGAWTGGRNLIKRDVEKLKTH"
BASE COUNT          673 a          747 c          779 g          773 t
ORIGIN      
        1 catgctgtga ttttgctggg cgatgaatac atcaacttca ccctcttaca agtattcatc
       61 ccatttagca cagtagatta ccgcccgttt tgggtgggga tcggtcagct tgggttctat
      121 atttggttga tcgtggcgat gagtttttat gtgcgcccta tgatcgggca gaagttttgg
      181 cgtgtgctgc actacttgag tttcgccatg tattttgtgg gtattttcca cgccatcttc
      241 agcggaacag actccagcat gagttgggca cagaactatt attggttctc tgcaggcagt
      301 ttgctcttct tattctttgt gcgcatcgtc agcattgtga tcgatggctt attccccgtc
      361 aagaaacccg tcccagttca acgatagatt tgaacaacat gccaaataaa gagactgtca
      421 ctttcaccgt gacagtctct tttgatttag accttatcca aatagtagag ccattatttg
      481 ccgatgtatt gcaacgccac gcgcaaacgt tcttcgacat ctttgggagc atctttggga
      541 agcgcttgta atgcggcttg cgcttcgacc accgagtaac ccaatgcggc aagcgcggcg
      601 agaacttcgc tatcgtagtc ggcaagagct gccattttgg aaagcgcatc ggtcggctta
      661 agtttgtctt tgaggtgaat cgccattttt tgcgcggtct ttttgcccac gcccggaact
      721 ttgctaagca attcttcttc gtcggcaaag atggcgcgtt gaatcgtttc aagcgtgagc
      781 gtggataaca ccgagagcgc caccttggga ccgacaccat caacgccgag caagatgttg
      841 aagaggtcgc ggtctgattg ggattcgaag ccataaagag tcagcgcatc ttcccggacg
      901 acgagatggg tgaacaaaaa gatcatttct cccgccgaag cctttgtgcg catcggcgcc
      961 gggacgaaga ctcgcatgcc cacaccgcct acttcgacga tgatggcatt atcttcaacg
     1021 tgtgatactt ctccgcgtaa ggtggcaatc atgtttgtat tttaccgtaa ataaaatgta
     1081 ggggcgactc gcgagtcgcc cctacataat taatcatatc gagctgtatt caaatgcgtg
     1141 atggcaattg ctaatgcatc ggcggcatcg tcaggtttgg ggattttctc taactgcaat
     1201 aaagcgcgca ccatttcttg cacttgtcgc ttctcagcgg agccgtaccc cgctaccgct
     1261 tgtttgactt catttggggt gtattcgtag gtagaaatgc cggctttctc aagtgccagc
     1321 aaaatcacgc cgcgggcttg tcccaccgcg atgccggtgg tgacattctt cgagaaaaat
     1381 aatttttcta cggcagcagt ttcgggttta tgtttcttca gaagcttatt gagatcgtca
     1441 aagagcatca ccaggcgagc ggaggcggtg gcttctttcg gggtgaggat cacgccataa
     1501 tcaacagcga ccagttcccc gtctggcatg aggcgcacga atccgtagcc ggtggtggcg
     1561 gtgccggggt caattccgag ggcgagagtc atatcattaa atgatgtagg ggcgggtctg
     1621 agacccgccc ctacgtaaaa ttgaattacg cgttttcgag ggctgcgagt gcttcgtcag
     1681 agactttgac gttgtgatag acatcttgca catcgtcgag gtcttcgagg ttttcgacga
     1741 ccttcattac tgccatgatc tcttccacgc cgaggtcgat ctcttgtttg gcgatcaaac
     1801 gtagaccggc ttccgcaggc tgcactttgg ctttgccgag cgtatccatg atggttttga
     1861 aggcttctac gggaccaaag atttcgatct cttcgccgct atccacgaca tcgtcggctc
     1921 cggcttccac accgagttca aaggctttat cgaacgccat ggcgctggag gggaaggaga
     1981 agtaggattt gcggtcaaat tgccagccca cggctccggc ttctgccatg ttgccgccga
     2041 acttgctgaa gatgtgacgt acatccgaaa cggtgcgtac gcggttgtcg gttacgcagg
     2101 agaccatcac agccgagcca tgcggaccgt agccttcgag agtgagttct tcgaacacgg
     2161 cagcatcttt gtcttcgccg gtgcctttct taatggcacg ttcgatgttg tctttaggca
     2221 tattttcagc gcgggctttt tctacagcca ggcgcaagcg gaagttggta tcggggtcgc
     2281 cgccttcacg tgcagcaatg gtaatttcac gcgccaagcg ggtgaagatg gcgccgcgtt
     2341 tggcgtcggc aacacctttt ttgcgtttaa tggtggacca tttagaatgt ccggacatgt
     2401 cgttctcctt cagataatga ccaatttagg ttggggaatg atgaattaag cggcgaaatt
     2461 ataccattct ggtttcgcgg catggtaggt taaaaccctt ctgagtatgg ctgtagcaaa
     2521 gtcataaatc attaatgtga aaaacgcgaa aaaagaggag accatgcgct cttcgcggtt
     2581 aatgagtctt gagtttttcc acatcgcgct ttatgaggtt tcgtccaccg gtccatgccc
     2641 ctacagcggt cgaaaggaca gagaacagaa agctgaatag aatcgccgct tcaaaacgcc
     2701 cggcaaagat gacgttcaaa atcagcacat ccaatttcaa aacgctctgg cggaaagatt
     2761 ccggcaggaa gatgccaaag atcagcccaa gaaaggctcc aaacgccatg cccgtatagg
     2821 cacccgtgct gaccccaccc gttatccctg cagtgcggat gaccagtgct gcagtgagtg
     2881 caccgttaat tgcaccggtc acaagcccca tgatggcagc cgttgtgatg ccattccaag
     2941 tgccgctaat cgcacctgag cctgccccca ga
//
LOCUS       DAOQNJ010000331         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24015_length_2971_cov_15.073388, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000331 DAOQNJ010000000
VERSION     DAOQNJ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24015_length_2971_cov_15.073388"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLG52_09500"
     CDS             complement(<1..578)
                     /locus_tag="PLG52_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41722.1"
                     /translation="MNVLALKLILAPLIIGSASLAGRRWGPAVSGWIVGMPLTSGPVV
                     FFVALSHGATFAESSVLGVLSGGISLVLYALTYAWVATRFPWYVAFVGSFIVFSLSTL
                     FLQNITVSLWFIVPVVVAAILIGLTYMPKGEVEPGESKLSQWDIPVRILIGTSFILLL
                     TGIAPFIGSRLTGLLTTIPLYVTILSIFAHRD"
     gene            complement(578..1192)
                     /locus_tag="PLG52_09505"
     CDS             complement(578..1192)
                     /locus_tag="PLG52_09505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNB41723.1"
                     /translation="MKSKLFLAIPPYIAVWVGMFVLKNAWAALLGFHIAILIVVFLLK
                     PQYSFRTFFQSTSKKLFFFTILLCALSGPSLYLLRDFLGISPSLRVQLTALGLQGNVW
                     EWFILYFSIINPLMEEFFWRIVLGSDIKSFYLGDLVYAGYHTMIVWNKTSPLSIVLVL
                     LALTFIGWLWRQLYRKDGSLLTPVVSHSIADLSILLAIFFLVNP"
     gene            complement(1200..2123)
                     /gene="mvk"
                     /locus_tag="PLG52_09510"
     CDS             complement(1200..2123)
                     /gene="mvk"
                     /locus_tag="PLG52_09510"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNB41724.1"
                     /translation="MKSSAPGKIILFGEHAVVYNRPALAVPVNQVQVDVEVLDSDKPG
                     VWINAPMVNLHGELSTLPSDHPIGSVILKVFQHLGISPQPDLKLTIYSTIPVAAGLGS
                     GAAVSVALIRSLSSFLNHALSNEQVNELVFEIEKLHHGTPSGIDNTVITYNMPVYYMK
                     GKPIETFKCGKPFTIVIGDTGVPAPTKESVGDVRRLWQRDTNHFENIFNEVAQISTMA
                     RHFIESGRPEHLGELMDHNHEFLQKMTVSSPELDRLVDAARKAGALGAKLSGGGRGGN
                     MIALVNQDSAELVAAALMSAGAKRTVITEVK"
     gene            complement(2120..>2971)
                     /locus_tag="PLG52_09515"
     CDS             complement(2120..>2971)
                     /locus_tag="PLG52_09515"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNB41725.1"
                     /translation="KTMRPRQWTKNVFIFAALVFDKQLFIVDSFLRTLAGFALFCLIS
                     SCVYIFNDLADVEADRQHPEKKNRPIASGKLPVSAAWTAGIVFVILTLGLAYLLAPTF
                     AGVVAGYFLLNLAYSNYLKHIPILDVLIISIGFVLRVGAGVTLIAVERFSPWLYIVMF
                     LLSLFLGFGKRRAELALLAHGAGSHRKVLDGYTLPLLDQYIMIVSGTTIVAYSLYTFS
                     APNVPENHSMMLTIPFLVYAIFRYLYLIEVEHAGGTPEEVLLSDRPFQISMLLWAVTV
                     IIIFYFS"
BASE COUNT          929 a          657 c          736 g          649 t
ORIGIN      
        1 taatcacgat gggcgaaaat acttaggatg gtgacataaa gtgggatggt ggtgaggagc
       61 ccggtcaggc gggaaccgat aaatggggca ataccggtga gtagcaaaat aaagcttgtg
      121 ccaatcagaa ttcgcacggg aatatcccac tggctcaatt tgctttctcc cggctctacc
      181 tctcctttgg gcatgtaagt taatccaatt aagatggctg ctacaaccac aggcacaatg
      241 aaccaaagag aaacagttat gttttgtaaa aataaagtac tcaacgaaaa aacaatgaag
      301 ctccctacaa acgcgacata ccaagggaaa cgtgtggcaa cccatgcata tgttagggca
      361 tacaacacca gcgaaattcc gccactgaga acacctaaaa ctgaactttc tgcaaaggtc
      421 gcaccatggc ttaacgccac aaaaaatacc accggacctg atgtaagtgg catcccaacg
      481 atccaaccgc taacggcagg cccccaccgg cgacctgcca aactggcaga accaataatg
      541 agaggcgcaa gaattagttt tagagcaaga acattcatta gggattcacc agaaagaata
      601 tagccaacaa gatcgaaaga tctgcaatag aatggctcac aacgggcgtg agtaaacttc
      661 cgtctttgcg atacagctga cgccaaagcc agccaataaa ggtcaatgca agaagtacaa
      721 gcacaattga tagcggagac gttttattcc acacaatcat agtgtgataa cccgcataaa
      781 ccagatcgcc caagtaaaac gattttatgt cgctccctaa aacgatacgc caaaaaaatt
      841 cttccataag ggggttgata atcgaaaagt ataaaatgaa ccattcccaa acattgccct
      901 gtaaaccaag agccgtcagt tgcacgcgaa ggctcggaga aatccctaaa aaatcacgca
      961 gtagatataa actaggtccg cttagagcac agagtaatat agtaaagaaa aataattttt
     1021 tactcgtgct ctgaaaaaaa gtacgaaaag agtattgggg ttttagtaaa aatacgacaa
     1081 tcagaattgc gatatgaaaa ccaagtaaag ccgcccaggc attttttaaa acaaacatcc
     1141 ccacccaaac agcaatatag ggcggaattg ctaaaaacaa ttttgatttc ataggaggat
     1201 tacttaactt cggtgatgac tgtccgtttt gcgcctgcgg acatgagtgc tgccgctacc
     1261 aactccgctg agtcttgatt caccaacgca atcatattgc cgccgcgtcc gccaccgctg
     1321 agcttggcgc caagtgcccc tgctttacgg gctgcatcca caagcctatc cagttcaggc
     1381 gaagagacgg tcattttttg gagaaactcg tgattgtggt ccatcaactc accgagatgc
     1441 tcgggacgtc cgctttcgat gaagtgacgt gccatggtgg agatctgcgc tacttcgttg
     1501 aagatgttct cgaagtggtt tgtatccctt tgccatagac ggcgcacatc ccctacggat
     1561 tctttggtgg gcgcaggaac ccctgtatcg ccaatcacaa tggtgaaggg tttgccgcat
     1621 ttgaaggttt caatcggctt gcctttcata taatacacag gcatattata ggtaatgacc
     1681 gtattatcta tgcctgaggg tgtgccgtgg tgaagttttt caatttcaaa gacaagttca
     1741 ttgacttgtt cgttagaaag cgcgtgattg agaaaagaag ataatgacct aattaaagca
     1801 acggaaactg cggcaccaga gccgagacct gcagcaacag ggatggtaga gtagatggtt
     1861 agttttaggt caggctgtgg ggagatgcct aagtgttgaa acactttcag aatcaccgaa
     1921 ccaattggat ggtcggaagg aagcgtggaa agctcgccat gcaagttcac catcggggca
     1981 ttgatccaaa cgccaggctt gtctgagtct aatacttcca catccacttg cacttggttg
     2041 acagggacgg caagcgcagg acggttgtat acaacggcat gttcgccgaa gagaatgatc
     2101 ttgccggggg cggaagattt catgaaaagt aaaagatgat aatgacggtc actgcccaaa
     2161 gcagcatgga gatttggaag ggacgatcag aaagcaaaac ttcttctggc gtgccaccgg
     2221 catgttctac ttcgattaga taaagatatc ggaagatggc atacaccaga aatgggatag
     2281 tgagcatcat ggagtgattt tcgggcacat tgggtgctga gaaggtatag agtgaatatg
     2341 ccacaatggt tgtgccggag acgatcatga tgtattggtc gagtaagggc aaagtatagc
     2401 catcgagcac tttgcggtga gaacctgccc catgagccaa cagggcaagc tcggcgcgac
     2461 gtttgccaaa gccgaggaaa agcgaaagca ggaacattac gatataaagc cagggagaga
     2521 aacgctccac tgcaatgagc gtcacaccgg cgcccacgcg cagaacgaag ccaatagaga
     2581 tgatgagcac atcaagaatg gggatgtgct ttaggtaatt tgaatacgca aggtttagaa
     2641 ggaaatagcc cgcaaccacg cccgcaaacg taggtgctaa taggtaagca agacctaaag
     2701 tgaggatgac gaaaacgata cctgccgtcc atgcggcgct aaccggaagt ttgccagagg
     2761 caatgggacg attctttttc tcggggtgtt ggcggtctgc ttcaacatct gcgaggtcgt
     2821 tgaagatgta aacgcaagaa gaaataaggc agaacaacgc aaaacctgcc aatgtgcgta
     2881 gaaatgaatc aacgataaat agctgcttgt cgaagaccaa tgcggcaaaa atgaaaacat
     2941 ttttcgtcca ttggcgcgga cgcatggttt t
//
LOCUS       DAOQNJ010000332         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24061_length_2968_cov_6.728802, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000332 DAOQNJ010000000
VERSION     DAOQNJ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24061_length_2968_cov_6.728802"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PLG52_09520"
     CDS             <1..802
                     /locus_tag="PLG52_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNB41726.1"
                     /translation="AAPLRVGPLVQKHLWYEKASVILASATLTTHGEFTYIRNTLDAA
                     EVDTLALGSPFDYESSTLLYVPSDMPEPNAFNYQQILDRSIIATAKATGGRMLVLFTS
                     YAALKKTSQAITGALAREDIFVFEQGEGTSPTALLESFKTTDRAVLLGTRSFWEGVDV
                     PGDSLSVVMITKLPFEVPSDPIISARSELYEDSFNEYYLPEAILKFRQGFGRLIRTAS
                     DRGIVAILDRRVITKQYGRMFLESLPQCTARQGPIANLPKMAGQWLGM"
     gene            873..1715
                     /locus_tag="PLG52_09525"
     CDS             873..1715
                     /locus_tag="PLG52_09525"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNB41727.1"
                     /translation="MPNIYFTLFEIIIYFQFVLCLNHARKQGAANLLKLAFGVLFGVS
                     LELATIRQLHAYEYGQFLLMVLDVPLCIGVAWSCIIYAAMEFSDASSLPYFFRPVLDG
                     LLALNIDLALDAVAIRFGFWDWGQGLTTQYFGVPYANFWAWFWVVFSFSLGYRLLAKN
                     TTWVGTWLSAPMAYFIGLIGVIGTNALIVFVVPATIRPGVIGLTLASALILVLIRRPS
                     FYLRPVHPVAFWVPFLTHIYILIAGLVSGMILAPVFLLFVGILMLAISFYLHGKTAKE
                     IFPQ"
     gene            complement(1766..>2968)
                     /locus_tag="PLG52_09530"
     CDS             complement(1766..>2968)
                     /locus_tag="PLG52_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNB41728.1"
                     /translation="IQRKMMQTMVRLTSIGNPQVSSINLELTQLVRSLAQLKEDTKAR
                     FKRQQQRLEALAEIGGVINSSLDTKHVLDAVMDSLILLMQAKRGFLMLRDETGDFQVQ
                     IARDADHQDLTENDFLYSKGIVRRVLRDGQAVLTTSAKDDPRFEQQASVAAYQLLSIL
                     CAPLKVKDELIGVIYVDNSAQSGIFGNDDLELITAFSNQAAVAIENAATYQSTLEGWV
                     RALDLRDKETEGHTKRVTQLTEVLARKMEVDNDSLVHIQRGALLHDIGKMAIPDGILL
                     KPAALTPEERAIIQKHPIYAFEMLSPIHFLHPALDIPYCHHEKWDGTGYPRGLSGEEI
                     PLAARIFAVIDVWDALVSDRPYRRGLDPADVKEKIREGAGKHFDPHVVNAFLDLDDQV
                     LSSNAKIS"
BASE COUNT          697 a          850 c          641 g          780 t
ORIGIN      
        1 cgccgccccg cttcgggtag gacctttagt acaaaaacat ctctggtacg aaaaagccag
       61 tgtgattctc gcttcggcaa ctctcaccac tcatggcgag tttacctaca tccgcaacac
      121 actcgacgca gccgaagtgg atacgctcgc gctcggctcc ccttttgact atgagtccag
      181 caccttgctc tatgtgccta gtgacatgcc cgagccaaat gcgttcaatt accagcaaat
      241 tctcgaccgt tccatcatcg ccaccgccaa agccacgggc ggacgaatgc ttgtgctgtt
      301 tacatcctat gctgctttga agaaaacctc gcaagccatt acaggcgctc tggcaagaga
      361 agatattttt gtctttgaac aaggcgaagg gacttcgccc acagccttgc tcgagtcctt
      421 caaaactaca gaccgtgccg tgctcttggg aactcgctct ttctgggaag gggtggacgt
      481 gcccggcgat tcgctctctg tggtgatgat caccaaatta ccctttgaag tgccgtcaga
      541 ccccattatt tcggcacgtt cagaactata cgaagattcc ttcaacgaat attacctacc
      601 cgaagccatc ctcaaattcc ggcagggatt tggtcgcctc atccgcaccg catcagaccg
      661 cggcattgtc gccattctcg accggcgtgt gattaccaaa caatatggca gaatgttcct
      721 agaatcacta ccccaatgca cagcccggca gggaccaatt gcaaatttgc cgaaaatggc
      781 aggtcagtgg ctgggaatgt aaaataagaa atatcaatac gtgacagtca tcttaaagat
      841 gactgtcact ttaataactc aaggtccaag ccatgcccaa tatctacttc accctcttcg
      901 aaattatcat ctattttcaa ttcgtcctct gccttaatca cgctcgcaaa cagggcgctg
      961 caaatttact caaacttgcg tttggcgttc tatttggagt ctccctcgaa ctcgctacca
     1021 ttaggcaact gcatgcttat gaatacggtc aattcctgct catggtctta gatgtgcctc
     1081 tttgcattgg cgtggcatgg agttgcatca tctacgctgc catggagttt tcagacgcca
     1141 gcagtctgcc ctacttcttc cgcccggtat tagacggtct cctcgccctc aacattgacc
     1201 ttgcactcga cgcggtagcc atccgcttcg ggttctggga ctggggtcag gggctcacca
     1261 cacaatactt cggcgtaccc tatgcaaact tctgggcatg gttctgggtc gtcttttcct
     1321 tctcgcttgg ctatcgtcta ctcgcaaaaa atacaacgtg ggtcggaaca tggctttctg
     1381 ccccaatggc atacttcatt gggctaatag gagttatcgg gacaaatgcc ctgatcgtat
     1441 ttgtcgttcc cgccacgatt cgccccggtg tgatcggatt gactctcgcc tccgccttaa
     1501 tcctggtgct gattcgccgc ccaagctttt atcttcgccc ggtccacccc gttgcatttt
     1561 gggttccctt ccttacccat atctacatcc tcattgcagg gttggtatct ggcatgattc
     1621 tcgcccccgt gtttctcttg ttcgttggca tcctcatgct agcaatttct ttttatctac
     1681 atggcaaaac agccaaagaa atatttcccc agtaaataat taaaataaaa agaggcagtc
     1741 actgaaaatt gactgcctct ttttattaac tgattttcgc gttagaactc aacacttgat
     1801 catccaaatc aaggaatgca ttcaccacgt gcgggtcaaa atgcttgcct gcaccctcgc
     1861 ggattttttc tttcacatct gccgggtcca gccctctgcg gtatgggcgg tctgacacca
     1921 acgcatccca cacatcgatc acggcaaaaa tccgcgccgc gagcggaatc tcttctccac
     1981 tcaaccctcg cggatacccc gtaccatccc atttttcatg gtgacaataa ggaatgtcca
     2041 atgcagggtg caggaagtga atcgggctga gcatttcaaa ggcgtagatc gggtgcttct
     2101 ggataatcgc ccgctcctcc ggagtcagag ccgcgggttt gagcaaaatc ccatctggaa
     2161 ttgccatctt gccaatatca tgcaaaaggg cgccgcgctg aatatggaca agactatcat
     2221 tatccacttc cattttgcgg gcaagcacct cagtcaactg agtgacacgt ttggtatgcc
     2281 cttcggtttc cttatcgcgc aagtccagcg cccgaaccca gccctccagc gtggactgat
     2341 acgtcgcagc attttcaata gccactgccg cctgatttga aaatgccgtg atcaattcca
     2401 gatcatcatt accgaagatc ccagattgag cgctgttatc cacatagatc acaccaatca
     2461 actcatcttt tactttcaac ggcgcgcaca aaatggatag gagctgatat gccgccacac
     2521 tcgcttgttg ctcaaagcgc gggtcatctt ttgcgctcgt cgtcaacacc gcctgaccat
     2581 ctctcagcac acggcgcaca atacctttgc tgtacaagaa gtcattctcg gttaggtcct
     2641 gatggtctgc atcgcgcgcg atctgcacct gaaaatctcc agtttcatcg cgcagcataa
     2701 gaaacccacg cttggcttgc atgagaagga ttagagaatc catcaccgca tccaacacat
     2761 gtttcgtatc aagagatgaa ttaatcacgc caccgatttc agccaaggct tccagtcgct
     2821 gttgttggcg cttgaagcga gcttttgtgt cttctttgag ttgcgccaag ctacgaacca
     2881 gttgcgtcaa ctcaagatta attgaagaca cctgcggatt tccaatactg gtcagtctca
     2941 ccatggtctg catcattttg cgttgaat
//
LOCUS       DAOQNJ010000333         2966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24095_length_2966_cov_9.681896, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000333 DAOQNJ010000000
VERSION     DAOQNJ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24095_length_2966_cov_9.681896"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLG52_09535"
     CDS             complement(<1..339)
                     /locus_tag="PLG52_09535"
                     /inference="COORDINATES: protein motif:HMM:NF016413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HNB41729.1"
                     /translation="MSTRYKIRQNLISIGDDFWIENEEGKRVFKVDGKVLRIRKTLVF
                     EDANGKKLAQIQERLLTIKDTMVVEDADGKDLATIKKALLTPFRDRWDVKVKNGPDLD
                     VQGDFLAHEYT"
     gene            complement(430..768)
                     /locus_tag="PLG52_09540"
     CDS             complement(430..768)
                     /locus_tag="PLG52_09540"
                     /inference="COORDINATES: protein motif:HMM:NF022279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HNB41730.1"
                     /translation="MDALKQLNLALRFLLELCMLAAVGFWGFKTHQTWFPKILFGIGF
                     PLLIAIVWGTFLAPRATRPLTGASFLTLELILFSSGAIALFASGKPTLGWVYTIALVV
                     NKVLLIVWKQ"
     gene            complement(782..1357)
                     /locus_tag="PLG52_09545"
     CDS             complement(782..1357)
                     /locus_tag="PLG52_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HNB41731.1"
                     /translation="MPRKTRTRLDKNAVVQAAVEILNAEGVQALTLSRLAEELGIQTP
                     SLYNHVEGLPGLQQELAILNAKLLADRLSSAAMGKSGAELFMEASQAFRAYVKEYPGL
                     YMSTLRSSGAQETPNKELQLEEERTVNVGLVVMASLGLKGENAIHGLRAFRSMVHGFA
                     TLEVAGGFGLPQDCDESFRRLVLALVAGLQK"
     gene            complement(1360..2160)
                     /locus_tag="PLG52_09550"
     CDS             complement(1360..2160)
                     /locus_tag="PLG52_09550"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB41732.1"
                     /translation="METRFFNYKTGQIAYEVQGEGPLVVCVPSLGDVRGEYRFVVPEL
                     VKAGYRVATMDVRGHGETSTTWDDFSVAAIGEDVLALIRELKAESAIVVGTSMGGGAA
                     IWSAVEAPELIRGMLLIDPFVDGDSNPFFIFLLSIMFARPWGAAMWKKYYSSLYPTRK
                     PADFDLYVETLYKNINQPGRLEAVMAMMRASKRSSGERMPNVKQPALILMGSKDPDFK
                     NPIAEAKRVAEAVRGNYKIIENAGHYPHAEIPEVTIPLMLDFMNSLKG"
     gene            2281..2814
                     /locus_tag="PLG52_09555"
     CDS             2281..2814
                     /locus_tag="PLG52_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNB41733.1"
                     /translation="MSFKVSVYIATSLDGFIARKNGDLDWLPIPTEGGEDYGYAQFMS
                     TVDVIVMGRNTFEKVISFGGWHYDKKVFVLSSRRLDIPSELNEKVEALHQTPRELIRE
                     LERRGIRHIYLDGGVTIQRFLREGLVNEMIITTIPVLIGEGLPLFGELEKDIQLELLN
                     VSSFSNGLAQKKYKVLT"
BASE COUNT          786 a          703 c          671 g          806 t
ORIGIN      
        1 ggtgtactca tgcgcgagga agtcaccttg cacatcgagg tcaggtccgt tcttcacttt
       61 cacatcccaa cggtcacgga agggcgtgag aagcgctttc ttgatggtgg caaggtcttt
      121 gccatcggcg tcttctacca ccatggtgtc tttgatagtg agcaaacgtt cctgaatctg
      181 agcgagtttc ttgccgtttg catcttcaaa gaccagagtt ttgcgaatgc gcaagacctt
      241 tccgtctact ttgaaaacac gttttccttc ttcgttctcg atccagaaat catctccgat
      301 ggaaatgagg ttttggcgaa ttttatagcg ggtggacatg gcgaaattcc ttttgaaatt
      361 aaattttttc aatcagtata atccatgtac gatagtgact taactaagtt gcaattaatt
      421 cgaaatgagt tattgcttcc aaacaatcag taaaactttg ttgacgacca aagcaatggt
      481 atatacccag ccgagggttg gtttgcctga agcaaacagg gcaatagcac cagaagaaaa
      541 cagaatcagt tccaaggtca ggaaagaagc gccagttaaa gggcgagttg ctctaggggc
      601 aaggaaagtc ccccacacga tggcgatcag aagagggaat ccaattccga aaagaatctt
      661 tggaaaccaa gtttggtggg tcttgaaccc ccagaagcct accgcggcaa gcatgcacaa
      721 ttccaataaa aatcttaatg ctaaattcaa ttgttttaaa gcatccatgt tttactccaa
      781 attatttctg tagcccagcc accagagcga gtaccagcct gcgaaaactt tcatcgcaat
      841 cttgcggcaa gccaaaaccg ccagccactt ctaaagtggc gaaaccatgc accatgctgc
      901 gaaaggcacg taaaccatga atggcatttt cgcctttcaa tcccaatgaa gccataacga
      961 ccaaaccgac attcacggtg cgttcttctt ctagctgtaa ttctttattt ggcgtctctt
     1021 gcgcacctga tgaacgcagg gtcgacatat acaaacccgg atattctttc acataagcac
     1081 gaaaagcttg tgatgcttcc atgaataatt cagcgccgct cttccccatc gccgctgaac
     1141 tgagtcgatc tgcaagaagt tttgcattga gtatggcaag ttcttgttgc aagccaggca
     1201 acccttctac atgattgtac aaagatggcg tttgaattcc taattcttcg gcaagacggc
     1261 taagtgtgag cgcttgcacg ccttctgcat tgagaatttc tacagcagct tgcacaacag
     1321 catttttatc aagtcgagta cgagttttgc gtggcatggt tatcctttca gtgaattcat
     1381 aaagtcgagc atcagcggga ttgtcacttc aggtatttca gcatgcggat aatgcccggc
     1441 attttcgatg attttataat tgccgcgcac tgcttctgca actcgtttcg cttcggcaat
     1501 cgggtttttg aaatcagggt ctttgctgcc catcaagatc aacgcaggtt gttttacgtt
     1561 gggcatacgt tcccctgaag aacgtttaga agctcgcatc attgccatca ctgcctctaa
     1621 ccgccctggc tgattgatgt ttttatacaa tgtctcaaca taaaggtcga aatcagcggg
     1681 tttgcgcgtt ggatataagg acgagtagta tttcttccac atcgctgcac cccacgggcg
     1741 agcaaacatg atcgaaagca agaaaatgaa gaaaggattg ctatcgccat ccacgaacgg
     1801 atcgatgaga agcatgccgc gaattaactc tggcgcctct actgctgacc agatggcggc
     1861 tcccccgccc atggaagttc ccaccacgat agctgattct gctttcaact cgcgaatgag
     1921 tgcgagaaca tcttcaccga tggctgccac tgagaaatca tcccaagtgg tactcgtctc
     1981 gccatgcccg cggacgtcca tggtagcgac gcgatagcca gccttgacca gttctggtac
     2041 gacaaaacga tattcgccgc gcacatctcc taaagacggc acacatacca ctaatggacc
     2101 ttctccctgc acctcatagg caatttgccc ggttttataa ttgaaaaatc ttgtttccat
     2161 cgttctctcc atactactaa ttatattagt attttaacta aatatattag tttttgtcaa
     2221 gagggaactt ttttgtacgt tatggcgttt attcacttca actgtttatg aggaggaagc
     2281 atgtctttta aagtttctgt gtatattgcc accagtctcg acggtttcat cgcccgcaag
     2341 aacggggacc tcgactggct gcccatcccc accgaaggcg gcgaagatta cggctacgct
     2401 caattcatgt ccacggtgga tgtgattgtg atgggacgca acacctttga aaaagtgatc
     2461 agttttggtg gttggcatta cgataaaaag gtcttcgtac tttccagccg taggttggat
     2521 atcccttcgg agctaaacga aaaagttgaa gcgcttcacc aaactccccg agagttgatc
     2581 cgcgaactgg agcggcgcgg catccgacat atttatctcg atggcggtgt gaccatccaa
     2641 cgatttttgc gcgaagggtt ggtgaacgaa atgattatca ccacgatccc tgttttgatc
     2701 ggtgaaggtt tacctctgtt tggtgaatta gaaaaagaca ttcagctgga attactgaat
     2761 gtgagttctt tctcgaatgg gcttgcgcaa aagaaataca aagttcttac ataaaaaaca
     2821 aaagagcgtg attggtatca cgctcttttg tttcaagatt agaacttcaa ttgatctatc
     2881 gccgagcgga ccacgctggc gctatgccgg agtgcatcca tttcgttcgg gtctaaattg
     2941 gggaagaacg tttggatgat gccttc
//
LOCUS       DAOQNJ010000334         2957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24230_length_2957_cov_6.277739, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000334 DAOQNJ010000000
VERSION     DAOQNJ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24230_length_2957_cov_6.277739"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLG52_09560"
     CDS             complement(<1..269)
                     /locus_tag="PLG52_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNB41734.1"
                     /translation="MESSIGNKQPPDRLDDIDRHIINALRADGRVAFAQLAEQLNVSP
                     GMIRQRYNRLVDLGYLKVVAVTNPLMMGKRTMSMIGIRTDGRKMM"
     gene            complement(426..1586)
                     /locus_tag="PLG52_09565"
     CDS             complement(426..1586)
                     /locus_tag="PLG52_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB41735.1"
                     /translation="MLRKSLFNILAVLAILSFALTACGGGGGDAEAPAAGDGPQVTSS
                     GFECPEPNPRLDVTSTELNIFVWTEYFPQDMLDCFELVYGITLNRDEYSSNEEMYAKV
                     AAGGTAYDLAQPTDYIVPLMIRQELVQELDHSKLPLLSNFDPNWMDQPFDPGNKYSLP
                     YLAGTDAIVVNTATVENVPTSWADLWNPEYAGRMVFLDDSRAVIGLTLVTLGYDPNTT
                     DPAQLEEAKNKLAELVPNIKLFDSDSPKTALIAGDVDLGMTWTGEAFIANQENPDIQY
                     VYPEEGPVLWQDNWVMLKDASHADAAYAWMSYIMQGDVFWLTIRDFQYTNPNAAAIEY
                     AKNNQPEVYDAYINSPITNPPAEAIAKGFGIEDVGDATPLYDNIWVEIKGGN"
     gene            complement(1644..2543)
                     /locus_tag="PLG52_09570"
     CDS             complement(1644..2543)
                     /locus_tag="PLG52_09570"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41736.1"
                     /translation="MNPYRRSPGVIAATVFVYFFLYAPIVMLILYSFNSSRANVKFEG
                     FVTSFSDRVKMDGSTVVSSPCGPFHWYCDLAKNDDVIEAAGNTLSIAFTATIISTIIG
                     TMAALALQRYDFKIKPFSQLALYIPIVIPEIVMGIGILTLFSQAFGWLNNALNLTGDA
                     RLALGIGTVTVSHIAFMVPFVTLVVQARLQGFDKSYEEAAMDLGANEWITFRRVTFPM
                     ILPGVLSGALLAFTLSLDDFVITFFTNGPGSTTLPIYVYGLLRRIITPQVNALSTVWI
                     LTVFIAVVVLQWIQSREQKPSHS"
     gene            complement(2584..>2957)
                     /locus_tag="PLG52_09575"
     CDS             complement(2584..>2957)
                     /locus_tag="PLG52_09575"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41737.1"
                     /translation="LPFMILPIYTSLEKIDNSLYEAAADLGANAFRTFMRVTLPLSMP
                     GVVAGTILVFIPVMGTFIVSDILGGRQVILVGNLIQRQFLDARDPTFGSAASLILMVL
                     TLIVTYFYTRKFGFGEEIIAA"
BASE COUNT          820 a          722 c          716 g          699 t
ORIGIN      
        1 tccatcattt tgcgcccatc ggtacgaata ccaatcatgg acatggtgcg cttgcccatc
       61 atgagcgggt tggtcactgc caccaccttg aggtagccaa ggtcaaccag acggttataa
      121 cgctgccgga tcatgccggg cgacacattg agctgttcgg caagttgggc aaaagccacc
      181 cgcccatcgg ctcgaagagc atttataata tgacgatcaa tgtcatcgag gcgatctggg
      241 ggttgcttat taccgatact gctttccaca ccctcattct ataacgatta ttcatttttg
      301 tcaacgtttt tatgcttatt tttcaatatt aatgcatttt ttataattat ttttctgttt
      361 agcgtcaaaa aaacaccctc aaccacgcag ctgagggtgt tttttcaatg tataggaaga
      421 aaaggctaat tgccgccttt gatctctacc cagatattat cgtacagcgg ggtggcgtcc
      481 cccacatctt caataccaaa acccttggca attgcttcag caggcgggtt ggtgatcggc
      541 gagttgatat atgcatcata cacttctggc tggttatttt ttgcatattc aatggcagca
      601 gcattcgggt tggtgtactg gaagtcacga atcgtgagcc agaacacatc gccttgcatg
      661 atgtagctca tccatgcata ggcggcatca gcgtgagaag cgtcctttaa catgacccag
      721 ttatcctgcc aaagaacagg accttcttca gggtagacgt attgaatatc aggattttcc
      781 tggttggcaa taaaggcttc accagtccag gtcatgccga ggtcaacatc gccagcgatg
      841 agggcggttt tggggctatc gctatcgaag agcttgatat tcggaacaag ttctgccaac
      901 ttattcttgg cttcttccaa ttgagcgggg tcggttgtgt tggggtcata gccaagggtt
      961 accaaggtga gaccgatcac agcacgagag tcatccagga agaccatgcg tccggcatac
     1021 tctggattcc acaggtctgc ccaggaggtc ggtacatttt cgacagttgc agtgttcaca
     1081 acaatagcgt ctgtgccagc aagatatggc agtgagtact tgttaccggg gtcgaatggc
     1141 tgatccatcc agttcgggtc aaagttgcta agaagtggaa gtttggagtg gtccagttcc
     1201 tgaaccaatt cctgacggat catgagcggc acgatgtaat cggttggctg agccagatcg
     1261 taggcggtac caccagctgc aaccttcgcg tacatttcct cattggagga gtattcgtcg
     1321 cggttcaatg tgatgccata taccagttca aaacagtcca acatatcttg tgggaagtac
     1381 tccgtccaca cgaagatatt caactcggtg gaggtaacgt ccaaacgcgg atttggttcc
     1441 gggcattcaa acccagaaga ggtcacttga ggaccatccc cggcggcggg cgcttccgca
     1501 tctccaccgc ctccaccaca ggcggtcagc gcaaagctga ggatcgccag aacggcaaga
     1561 atattgaata gcgattttcg taacatgttt cttctccttt atatagttag caacgtgcgg
     1621 gtctcacgca cgatataccc agattaggaa tgagagggtt tctgctcgcg gctttgaatc
     1681 cattgcaaga ctacaaccgc aataaaaacg gtcaaaatcc aaactgttga aagcgcattc
     1741 acttgcggcg taataatgcg ccgtaacaga ccatatacgt agatcggcaa ggtcgtagag
     1801 ccaggtccat tcgtgaagaa ggtgattaca aagtcatcca aagaaagggt gaaggcaagc
     1861 aaagcacccg agagcacacc cggtaaaatc attgggaagg ttacacgccg gaatgtgatc
     1921 cattcgttag cgcccaaatc catggcggct tcttcgtaag acttgtcaaa gccttgcaag
     1981 cgagcttgca caacgagcgt tacaaacggc accatgaacg cgatatgact aacagtcacc
     2041 gtgccaatgc ccaatgccag gcgagcgtca cctgtcaaat tgagagcatt gtttagccat
     2101 ccaaaagcct gactaaacag tgtcagaata ccaatcccca taacgatctc agggatcacg
     2161 ataggaatat ataaagccag ttgcgagaaa ggtttaattt tgaaatcgta acgttgcagc
     2221 gcaagagccg ccatcgtacc aatgatggta gaaataatcg tagcagtaaa cgcgatgctc
     2281 aacgtattgc ctgcggcttc gatcacatca tcattttttg caaggtcaca ataccagtga
     2341 aagggaccac atgggctaga cacgaccgta ctgccatcca tcttcacccg gtcactgaac
     2401 gaagtaacaa acccttcaaa cttcacattc gcgcgtgaag agttaaagga atataagatc
     2461 agcatcacaa tcggtgcgta cagaaagaaa tacacaaaga ccgtggcggc gattacccca
     2521 ggggaacggc gatacggatt catcgcattt cctccttaat aaaaaggttt gtagtgtatg
     2581 ggatcatgct gcaatgatct cctctccaaa tccaaattta cgggtgtaga agtatgtaac
     2641 gatcagggtt agcaccatca aaataagcga agccgccgaa ccgaaggttg ggtcacgcgc
     2701 atccaagaat tgacgctgaa tcaggttccc caccagaata acctggcgtc cgcccaagat
     2761 atcagacaca atgaatgtcc ccatcaccgg gatgaatacc aagatggttc cagccaccac
     2821 gcccggcatg gaaagcggca aggtaacacg catgaaagtt cggaaggcat tggcgccaag
     2881 atctgcagca gcttcataca atgagttatc gatcttctca agcgaggtgt agatcggcag
     2941 aatcatgaac ggcaaaa
//
LOCUS       DAOQNJ010000335         2952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24308_length_2952_cov_10.420780, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000335 DAOQNJ010000000
VERSION     DAOQNJ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24308_length_2952_cov_10.420780"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /gene="msrP"
                     /locus_tag="PLG52_09580"
     CDS             <1..353
                     /gene="msrP"
                     /locus_tag="PLG52_09580"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNB41738.1"
                     /translation="MNDLTLLATGIYGETILPQNGAPIRLVVPWKYGFKSIKSIVKIE
                     LTDKEPTTLWSTIAPNEYGFYANVNPTVDHPRWSQASERRIGELTRRETLMFNGYGEQ
                     VADLYADMDLNLNY"
     gene            402..1082
                     /locus_tag="PLG52_09585"
     CDS             402..1082
                     /locus_tag="PLG52_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNB41739.1"
                     /translation="MKIFNRYTPLQIAIHLYAWSALVWIIAELFMGTFSINPIQELEQ
                     RTGRHAITLLVLSLLCTPLNTIFKFPELLKRRRTLGLYALLYATIHVLIFINLDYGLA
                     WSMIARTVFEKPYIIVGALAFLMLIPLGITSFDIWKVRLGKNWKRLHQMVYVIAPLVV
                     LHYLWSKKGDLLNFSGDIVRPTIYGLVVVIFLILRIPAIRRAIASLPNRGLTLPFKKN
                     PTPKADAS"
     gene            complement(1120..1350)
                     /locus_tag="PLG52_09590"
     CDS             complement(1120..1350)
                     /locus_tag="PLG52_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41740.1"
                     /translation="MTTTTQKRQIPWYLWPFAAIWKLLAVIVEMTGRLVAMILGIVLL
                     VVGVLVSLTIVGAIVGVPLGIIGLLLFFRGMF"
     gene            1471..2307
                     /locus_tag="PLG52_09595"
     CDS             1471..2307
                     /locus_tag="PLG52_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNB41741.1"
                     /translation="MNTKSTRSKKSTPAVIEIEQEEPVIESTPDSEDFITFKRSHFYS
                     VLVVLAFAVGLLLGFVIWGRGSNTVTVAADPAAAANNAAAQQPSGEIAEAPVTPQPQY
                     TRYDIPTEGYPSLGPDDAPITIVEFSDFQCPFCRRFHSETYQALLDAYPGQIRFVYRN
                     LPLTSIHPDAMPSAIASLCANDQNVYWEYHDKLFSSETLTRDTFIQYATDLNLNVDEF
                     SACLDSGKHDENIQQDMDFALNLGVQSTPTFFVNGLAIVGAQPLSSFTQIIDMELAGK
                     IP"
     gene            2426..>2952
                     /locus_tag="PLG52_09600"
     CDS             2426..>2952
                     /locus_tag="PLG52_09600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNB41742.1"
                     /translation="MKRFIAIAGNIGVGKSTLVQMLCNQMGWDPFYEPVTQNPYLADF
                     YKDMSSWSFHSQVFFLTHRLRSHLNLAQHPNSVIQDRSVYEDAEIFAHNLYQQGNMTD
                     RDYQTYRDLYETAVQFLPPPDLVIYLRASVDTLLNRINHRGRDYERTIAPDYLQSLND
                     LYEGWIDNFTLCPVLA"
BASE COUNT          770 a          860 c          586 g          736 t
ORIGIN      
        1 ccatgaacga cctgaccttg ctggcaactg gtatttacgg tgaaaccatt ctccctcaaa
       61 acggggcgcc aattcgtttg gtggtgccgt ggaagtatgg cttcaaatcc atcaaatcca
      121 tcgtcaagat cgaattgacc gacaaagagc ccaccacatt gtggagcacg attgcgccca
      181 atgaatatgg cttctatgcc aacgtcaacc caactgtcga ccatccacgt tggtcacaag
      241 cgtctgaacg ccgcattggc gaactgaccc gccgcgaaac gttgatgttc aacggctacg
      301 gcgaacaagt cgcagactta tacgccgata tggatttgaa cttgaattac taaagaaatg
      361 aaacgtcgga agcctcaaga cttccgacgt ttactaaggt catgaaaatc tttaatcgct
      421 ataccccttt acaaattgcc attcaccttt acgcttggtc tgccctggta tggatcattg
      481 ctgagctgtt catggggacg ttctcgatca accccattca agaattggaa caacgcacag
      541 ggcggcatgc cattactctt ttggtgctgt ctttattatg caccccgctc aacacgatat
      601 tcaaatttcc tgagctgctc aaacgccgcc gcaccttggg cttgtatgct ttgctctacg
      661 caaccattca tgtgctaatc tttatcaatc tcgattatgg tctagcatgg agcatgatcg
      721 ctcgcaccgt tttcgaaaag ccgtatatca tcgttggtgc attggcattt ctcatgctca
      781 ttccccttgg cataacttca ttcgatattt ggaaggtccg cctcggcaaa aattggaaac
      841 gccttcatca aatggtatat gtcattgcgc ccttggtggt gctgcattac ctgtggagca
      901 aaaaaggcga cctacttaat ttctcaggcg acatcgtgcg ccccacgatc tatggcttgg
      961 tcgttgtgat cttcctgatt ttgcgcattc ccgccattcg acgtgccatc gcctctctcc
     1021 cgaaccgcgg cttgactctc cccttcaaaa agaatcccac tcccaaagcg gatgcttctt
     1081 aatttaaata agggcgacct gacaggtcgc ccttacgctt taaaacatcc cgcgaaagaa
     1141 cagcagtaaa ccgattattc ccaagggcac acccacaatc gcaccgacaa tcgtcaggct
     1201 gacgagaacg ccaacgacaa gcagcacaat accgaggatc attgccacga gccgcccagt
     1261 catctctaca atgacagcca gcaatttcca gattgcggca aagggccata aataccaagg
     1321 aatttgtctt ttctgagttg ttgtagtcat aagaagaacc tccatgccta atatacgcaa
     1381 aataggacaa atggttctct aaaaagtaga atgaaaaata cttaagtact actgcttaaa
     1441 tccaaaacac aggactataa tcttgccact atgaacacaa aatctacccg gtctaaaaaa
     1501 tccacgcccg ccgtgatcga aatcgaacaa gaagagcctg tcatcgaaag cacgccagac
     1561 tctgaagatt tcatcacctt caagcgcagt catttttact ctgtgctagt cgtccttgcc
     1621 tttgctgttg gtctcttgct tggcttcgtc atttggggac gcggcagtaa caccgttacc
     1681 gtcgctgcag acccagccgc tgctgccaat aatgctgcag cccaacaacc ttccggcgaa
     1741 atcgccgaag cgcctgttac cccacagcca caatacaccc gctacgatat ccccaccgag
     1801 ggctacccca gcctgggtcc tgatgatgca ccgatcacca tcgtagaatt cagtgacttc
     1861 cagtgtcctt tctgccgccg cttccattcg gaaacatatc aggcattgtt ggatgcttat
     1921 cccggtcaga tccgcttcgt atatcgcaat ctacctctca cctccatcca tcccgatgcg
     1981 atgccctccg cgattgcttc cctctgcgca aatgaccaga atgtgtattg ggaatatcac
     2041 gataaactct tcagcagcga aactctaacg cgcgatacct ttattcaata tgccaccgat
     2101 ttgaacctaa acgtagacga gttctctgcc tgtctcgata gcggcaagca cgatgaaaac
     2161 atccagcagg atatggactt tgctctcaat ctcggcgtcc aatctacccc caccttcttc
     2221 gttaatggtt tggcaattgt aggcgcgcag ccgctttctt ccttcaccca aattatcgat
     2281 atggaacttg cgggtaaaat tccctaagct ttagcaatac aatctcaaaa ggcggacggt
     2341 atcgtccgcc ttttttctta aaactccgtg ccaaaattta tcccagtggt aagatgtaaa
     2401 taataaacca ctcagagaga atgccatgaa acgatttatc gccattgcgg gaaatatcgg
     2461 tgtaggaaag tccacgctcg tacaaatgct ttgcaaccaa atgggctggg atccattcta
     2521 tgagcccgtc acccaaaacc catacctcgc ggatttttac aaggacatgt cctcttggtc
     2581 atttcattcc caagtcttct tcttgactca ccgcctccgc tctcacctca acctggctca
     2641 acatcccaat tccgtcatcc aagaccgcag cgtgtatgaa gatgccgaga tatttgccca
     2701 caatctctac caacaaggca acatgacaga ccgcgattat caaacctacc gcgacctgta
     2761 cgaaactgcc gttcaattcc tgccgccacc agacctcgtt atttacctcc gcgcctctgt
     2821 agataccctc ctaaaccgca tcaaccaccg cgggcgtgac tatgaacgca ccatcgctcc
     2881 cgactatctg caaagcctga atgacctcta cgaaggttgg atcgacaact tcaccctctg
     2941 ccccgtcctt gc
//
LOCUS       DAOQNJ010000336         2945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24420_length_2945_cov_10.698962, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000336 DAOQNJ010000000
VERSION     DAOQNJ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24420_length_2945_cov_10.698962"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLG52_09605"
     CDS             <1..567
                     /locus_tag="PLG52_09605"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNB41743.1"
                     /translation="GMLHMAFAQMARFYDVPAGGYVGLTNSKLNDAQAGYETGMSGIA
                     GVLGGMDMFNIGGLIDALKTFDFAKAVIDDEIAQMMKRMKRGVSFSDEDLGVNLIKEI
                     GPGGSFITAKHTTSRMKTEAVMTKIADRDARSIWEKKGATDTHAKALKKAKDVMASNT
                     TPLISPEVDEKIRAAFPGLVPGMLEPIP"
     gene            593..1213
                     /locus_tag="PLG52_09610"
     CDS             593..1213
                     /locus_tag="PLG52_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNB41744.1"
                     /translation="MFRIFRRKEDKFQKLIEQQAEISFEGLKLLVKYLETSAPDVAEE
                     LSFKEKEADEVRRILIDELNRTFVTPFDREDIFSLSRSIDDVVDYADTTVVEMVILKV
                     SPTPYMQRIASLLKDAAYEIWQGVLRLPKHPNVALDHAQRAKALENRVEAVYREAVAD
                     LFSGPEDIHHVVEMLKLREVYRHLSNAADRGDEAANVIADIVVKRT"
     gene            1515..2699
                     /locus_tag="PLG52_09615"
     CDS             1515..2699
                     /locus_tag="PLG52_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNB41745.1"
                     /translation="MRTKNFAYHLLALFVIAATLLAACGAPAPTEAPAAATDAPAATE
                     AAAATEAPAADPMADLVAAAQAEGTLTVIALPHDWCNYGEAIDTFKAKYGLEVNELNP
                     DAGSGDEIEAIKANKDNTGPQAPDVIDVGFAFGESSKAEGLIQPYKVSTWDSIPAEAK
                     DPEGYWYGDYYGVLSFLVNTDVQPDVPQDWADLLDPKYNGQVALSGDPRTSNQAIQTV
                     YAAALANGGSLDDAQAGLDFFAQLNSAGNLVPLISNNGLVAKGETPVRITWDYNALAA
                     VDSFAGNPAAQVVIPATGRFAGVYVQAISAYAPHPNAAKLWMEFLYSDEGQTIWMKGY
                     CHPIREADMRERGVIPADLLAKLPDVSGAVFPSLDQLNAAKELITTNWDSAVGSDIQA
                     AP"
BASE COUNT          702 a          691 c          740 g          812 t
ORIGIN      
        1 ggcatgttgc atatggcatt tgctcagatg gcacgttttt atgatgtgcc tgcgggcgga
       61 tatgttggct tgacgaactc caaactgaac gatgctcagg cagggtatga aaccggtatg
      121 tctggcattg cgggagtgct cggcggtatg gatatgttca acattggcgg tttgatcgat
      181 gcattgaaga ccttcgattt tgccaaagca gtgattgatg atgaaattgc acagatgatg
      241 aagcgtatga aacgcggagt cagctttagc gatgaagatt tgggagtcaa tctcatcaaa
      301 gagatcggac ctggcggctc ctttatcaca gccaaacaca ctaccagccg catgaagacc
      361 gaagcggtga tgaccaaaat cgctgaccgt gatgctcgtt ccatttggga gaagaaaggc
      421 gctacggata cacatgccaa ggcgttgaag aaagccaaag atgttatggc ttccaatacc
      481 actcctctga tctcgcccga agtggatgaa aagattcgcg ctgcattccc tggcttagtg
      541 cccgggatgt tagagcctat cccgtaatac aaggaataaa agaaggattt gaatgtttcg
      601 tatttttagg cgcaaggaag ataaattcca aaaactgatt gagcaacaag cggaaatatc
      661 gtttgaaggc ttgaagttat tggtgaaata tcttgaaacg tcggctccag atgtagctga
      721 agaactgtcg ttcaaagaga aagaggcgga tgaagttcgt cgcatcttga tcgatgaact
      781 gaaccgcaca tttgtcacac cgtttgaccg tgaagatatc ttttcgctct ctcgttcgat
      841 cgatgatgtg gttgattatg ccgatacaac tgttgtagaa atggtgattc taaaagtcag
      901 cccaacacct tatatgcagc gaattgcatc tttgttgaag gatgctgctt atgagatttg
      961 gcaaggcgtg ctgcgtttgc ctaagcaccc aaatgtggca ttggatcatg cgcaacgtgc
     1021 caaggcactt gagaaccgtg tagaagcggt ataccgtgaa gcagttgcag atctgtttag
     1081 cggacctgaa gatattcacc atgtggttga gatgcttaag ctccgtgagg tgtatcgtca
     1141 tctttccaat gcagctgatc gcggcgatga agctgccaat gtgattgcag atattgttgt
     1201 aaaaagaact taaccggatg ttaatccctt ttctttttga gtttgttatt atccttgcgc
     1261 tgataattga atttagtaat gatgcgcgca acattcaaag tggtaatacc gatatcttgt
     1321 atgtgactcc catgtttatt ggtacaaata actacgttgg tcattaattc aaagtctcac
     1381 aagcttgttt gactttgaat attgataatg ctaaaatgtt aacgatttgt taatggaatg
     1441 gaggtgccgg gaagttaaga tagcgcaagt attccgttcg ttcgatcaat ctaaaatatc
     1501 ataaggagaa agagatgcgt acgaagaatt ttgcttatca tttgctcgcc ctgttcgtga
     1561 tcgccgctac cttgttggct gcttgtggtg ctcctgctcc cactgaagcc cctgctgctg
     1621 cgaccgatgc ccccgctgcc accgaagctg ctgccgccac tgaagcccct gctgccgacc
     1681 ccatggctga cctcgttgct gctgcgcagg ctgaaggaac tttgaccgtg attgccttgc
     1741 cccatgactg gtgcaattat ggtgaagcca ttgacacctt caaggcgaaa tatggtctcg
     1801 aagtcaatga actcaatcct gatgctggct ccggtgatga aatcgaagct atcaaggcga
     1861 acaaagacaa cacgggtcct caggctccgg atgtgatcga cgttggtttt gctttcggtg
     1921 aatcttccaa ggctgaaggt ttgatccagc cgtacaaggt ttccacctgg gattccattc
     1981 ctgctgaagc caaggatcct gaaggctact ggtatggcga ctactatggt gttctttcct
     2041 tcctcgtcaa taccgatgtc caacctgatg ttcctcagga ctgggctgac ttgctcgatc
     2101 ccaagtacaa cggacaagtt gctctctctg gcgatccccg cacatccaat caggctatcc
     2161 agaccgtcta cgctgctgct cttgcgaacg gcggttccct cgacgatgca caggctggtc
     2221 tcgacttctt tgctcagttg aacagcgctg gtaaccttgt tcctttgatt tcgaacaatg
     2281 gtttggttgc caagggtgaa actcccgtcc gcatcacctg ggactacaat gctctcgccg
     2341 ctgtggattc ctttgctggc aaccctgccg ctcaggttgt gatccccgcc actggtcgtt
     2401 ttgctggtgt gtatgttcag gcgatcagcg cctacgcccc gcaccccaat gctgccaagt
     2461 tgtggatgga attcctgtac tctgacgaag gtcagaccat ctggatgaag ggctactgcc
     2521 acccgatccg cgaagctgat atgcgcgaac gcggtgtgat ccccgctgac ttgcttgcca
     2581 agttgccgga tgtttctggc gctgtgttcc cctcccttga tcagttgaac gctgcaaaag
     2641 aattgatcac caccaactgg gactccgctg tcggttccga cattcaggct gctccgtaat
     2701 agttcgttct tgtaagaaat gcgagaccct gccttatggc ggggtctcgc cgcaattgaa
     2761 ccattttcga ggagttccct atggcagaaa ctgctcttcc caccaaaaag aacgttgctc
     2821 caaggttcga ttggaaaaaa tggttggggg ttacaccgtt tttccttttt gcacttttat
     2881 tcttaattct gccctctctt aaattatttg ttggggcatt taccgatgat gctgggcgtt
     2941 ttacg
//
LOCUS       DAOQNJ010000337         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24493_length_2939_cov_7.847087, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000337 DAOQNJ010000000
VERSION     DAOQNJ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24493_length_2939_cov_7.847087"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..407)
                     /locus_tag="PLG52_09620"
     CDS             complement(57..407)
                     /locus_tag="PLG52_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNB41746.1"
                     /translation="MRPKQQVFAMKFSDVYPLYIQKAERKNRTKAEVDQIICWLTGYT
                     QTTLQQQIKQEHDFETFFAQAPAIHPNTSLIKGVVCGVRVEEIEDPLMQKIRFLDKLI
                     DELAKGKAMEKILR"
     gene            complement(491..862)
                     /locus_tag="PLG52_09625"
     CDS             complement(491..862)
                     /locus_tag="PLG52_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNB41747.1"
                     /translation="MTKYSMTPLNSGTRIRTDHSTFGSVITSVARGQLVVGDELWEAT
                     ADGTEVKKGDKWLHVTSVDGVNLAEAGWMAYIHKGVVICNNFTEIEEPNPNPTPVFPE
                     SFTLTDPSGAKAEYKFVRIIE"
     gene            complement(859..1617)
                     /locus_tag="PLG52_09630"
     CDS             complement(859..1617)
                     /locus_tag="PLG52_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HNB41748.1"
                     /translation="MTERPYQPKPAIQQKGTLFLTNFKLPDVSFWQDDPTTTQPINFK
                     QMATMTRGVIIRAGQGTYADRVFSTSWKNAKNAGLIRGSYWFYDSRVNPKRQAEKWVE
                     ILGDDPGEMEMWCDYEDRYGGPFGGWRNWFDFMERIKTLLPEKKLGVYTGYYYFQEFA
                     AGVNYFAQYPLWIAWYNPTMPLIPPIWQDWTYWQFTDDGDGTLFGVESREIDLNYFNG
                     SEAEFLARYPAPQTTEATLIAKFGNTLVEYRRVS"
     gene            complement(1710..2210)
                     /locus_tag="PLG52_09635"
     CDS             complement(1710..2210)
                     /locus_tag="PLG52_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB41749.1"
                     /translation="MKKLTLSLTLLLSIFLIAACGGESTPQTQPETAAPTEVISQPTD
                     TALPAATDTSAPATVAPTETAAATEASTSTGVSYVADVKPIFDAKCIKCHGVESTKEG
                     LDMQTYENLMAGSRNGSVITPGNADDSELVRLIVRGKMPNRGQKLSDEEIQLISDWIN
                     QGALNN"
     gene            2378..>2939
                     /locus_tag="PLG52_09640"
     CDS             2378..>2939
                     /locus_tag="PLG52_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41750.1"
                     /translation="MIGEFTLPNEYWLKLQVTSQDVENLHTILFERETPLTTRDLVSE
                     FVDARIQAERKAAENKQKSAGKPFFPKEKYSAGDELVFPAVGWQRGKVASVRSGMNPA
                     VGEFDVITVDFEDSTQRFFAANLPSHALNDAPIEKPGANDINAAAIISEHGSALEKKI
                     ESAFGADEGLVKIAGRWFPRALLVDIN"
BASE COUNT          732 a          626 c          729 g          852 t
ORIGIN      
        1 ttgcgttacc tgcgctgggg tggggacggc gaagccgtcc agccagaaaa atgatgctat
       61 cgtaagatct tttccatcgc tttccccttt gcgagttcgt cgattaattt atctaaaaag
      121 cggatttttt gcattaatgg atcttcgatt tcttccacac gcacgccgca cacgacacct
      181 ttgataagag aagtgtttgg atgaatagct ggcgcttgag caaaaaaggt ttcaaaatca
      241 tgttcttgct taatttgttg ttgcagtgtt gtttgggtat agccagttaa ccagcaaatt
      301 atctgatcta cttcggcttt tgtgcggttc ttacgctctg ctttttgaat ataaagcgga
      361 tatacatctg aaaacttcat ggcaaaaact tgttgtttgg gtctcataac ttctccggtg
      421 tttcgatcta caatccccca atacgggagc gctgacagca tcaagctgtc aacgctcatt
      481 cctctaacta ttactcaata attcttacaa acttatactc tgctttggcg ccacttgggt
      541 ctgtaagtgt aaaagactcg gggaaaacag gtgtgggatt tggattcggt tcttctattt
      601 cagtaaagtt attacaaatg accacacctt tatggatgta tgccatccaa cccgcttcag
      661 caaggttgac accgtctact gatgtgacat gcagccattt atcgcctttt ttcacttcgg
      721 ttccgtcggc agtggcttcc cagagttcat cgccaactac caattgtccg cgtgcgacag
      781 aggtaatcac agacccaaat gtgctgtggt cggtacggat gcgagttccg ctgtttagtg
      841 gggtcatgct atatttggtc atgagactct cctatattca acaagggtat tgccaaattt
      901 ggcgatcagg gtggcttcgg tagtttgtgg agcagggtat cgagcaagga actcggcttc
      961 actgccgtta aagtaattta gatcgatctc ccggctctca acgccaaaga gagtgccatc
     1021 gccgtcatct gtaaactgcc agtaggtcca atcttgccag atgggtggga tgagcggcat
     1081 ggtggggttg taccatgcga tccataatgg atattgagcg aagtaattta caccagctgc
     1141 aaattcttga aaataatagt agcctgtata aacccctagc ttcttttctg gtaataaggt
     1201 tttgatgcgt tccatgaagt cgaaccagtt acgccagcct ccgaatggac caccgtaccg
     1261 gtcttcatag tcgcaccaca tttccatttc gccggggtca tcccctaata tttccaccca
     1321 cttctcggct tggcgtttgg ggtttacgcg gctatcgtaa aaccaatagg agccacggat
     1381 caaaccagcg tttttggcat ttttccatga ggtgctaaag acacggtctg catatgtccc
     1441 ttgcccggcg cggatgatga cgccacgggt catggttgcc atttgcttga agttaatggg
     1501 ttgagtggta gtggggtcat cctgccaaaa actaacatcg ggcagtttga aattggttag
     1561 gaacaaagtc cctttttgct gaatggcggg tttgggttgg taggggcgct ctgtcatggc
     1621 ttttggcaat tatataacaa ctattcggct aatgttaaaa gaaatcacca cagagttatc
     1681 tctgtggtga ttggtctttc tggcttttat tagttgttta aagcgccttg gttgatccaa
     1741 tcactgatga gctggatttc ttcatcagac agcttttgtc cgcgattcgg cattttgccg
     1801 cgcacgatca atctaacgag ttcgctatca tcagcattgc cgggcgtaat cacagaacca
     1861 ttgcgagacc cagccattaa attttcatag gtctgcatat ctaaaccttc tttggttgac
     1921 tctacaccat gacacttgat gcatttggca tcgaagatag gtttcacatc tgcaacataa
     1981 ctgacaccgg ttgaagtaga agcttctgtt gcggcggcag tttctgtggg tgcgacggtt
     2041 gccggggcgg aagtgtctgt agcagcggga agagcggtat ctgtcggctg agagataact
     2101 tcggtggggg cggcagtttc gggctgagtc tgaggcgtgc tttctccacc gcaggcagcg
     2161 atcaaaaaga tggataacag taaagtgagg gatagggtga gctttttcat gtcaagtcct
     2221 tttttaattt gaaggggcaa atttacgaga aagtgataaa aaatggatga agaaattgtg
     2281 tgaattctta caagtatgag agacctgtga gattttgcgc aaggaaggta taatcaatac
     2341 gcctcgtgag aggaattatt agacttcaag gcagaacatg atcggtgaat ttacactccc
     2401 caacgaatat tggttaaaac ttcaggttac atctcaagac gtagaaaacc ttcatacgat
     2461 tctatttgag cgcgagacgc cccttactac tagggatctc gtttcagagt ttgttgatgc
     2521 acgcattcaa gctgaacgca aggcggcaga gaacaaacaa aaatcagctg gcaaaccatt
     2581 tttccctaaa gagaaatatt cagcaggcga tgaactggtc ttcccggctg tgggttggca
     2641 gcgtggcaag gttgcttctg tacgttcggg tatgaatccg gcagtgggtg aattcgatgt
     2701 tatcaccgtt gattttgaag atagcactca gcgttttttc gcggcgaatt tgccttcaca
     2761 tgctttgaac gatgctccga tcgaaaagcc tggtgcaaat gatattaatg cggcagcaat
     2821 tattagtgaa catggctctg ctcttgaaaa gaaaattgag tctgcgtttg gtgctgatga
     2881 aggcttggtg aaaattgcag gacgttggtt cccacgcgct ttgttagtgg atatcaacg
//
LOCUS       DAOQNJ010000338         2936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24548_length_2936_cov_8.307185, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000338 DAOQNJ010000000
VERSION     DAOQNJ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24548_length_2936_cov_8.307185"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLG52_09645"
     CDS             <1..227
                     /locus_tag="PLG52_09645"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HNB41751.1"
                     /translation="PLLLDLCALALRLNKPLTARLMPVPGKKAGDATTFDFAFFANGR
                     VMALESQPLSLPFNSNESFSLKPRAERNRP"
     gene            317..1993
                     /locus_tag="PLG52_09650"
     CDS             317..1993
                     /locus_tag="PLG52_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HNB41752.1"
                     /translation="MKRNILIIGGLVFVAIVAFFVYKAVAPSKPVNVQLLALNDFHGN
                     LLPPSGSSGQVDGVDAGGVEYLATHIDTLRGENPNTVVVSAGDLVGASPLLSALFHDE
                     PTIEAFNLIGLDFNAVGNHEFDEGWAELKRLQEGGCHPVDGCLDGDDFSGAEFKFLAA
                     NVINKETGEPIFPAYEVREFEGVKIAFIGMTLKGTPSIVTPSGVADLEFLDEAETVNA
                     LIPELQAQGIETIVVLVHEGARQNGDAPTANTCQDIDGPIVDIVSRFDDAVDVVISGH
                     THQAYNCLLDGKLLTSAMSFGRLVTQIHLTIDPKTGDVTTMEANNLVVTRDVEKASLL
                     TALLERYNKVAEPLANHEVGTITADILREPNDAGEMALGDVIADSQLFATSASDMGNA
                     VIAFMNPGGIRTDLVYADSVGEGDGTVTFGEIFTVQPFNNNLVTLTLTGEQIDALLEQ
                     QFVDCGQTENPRILQVSEGFSYTWKASGAPCEKVDPATIFLNDEPIDPAKEYRVTVNS
                     YLADGGDRFKVLTEGTDRLVGVMDLDALEAYMLQASPVEPGAQDRIKVIP"
     gene            complement(2034..2591)
                     /locus_tag="PLG52_09655"
     CDS             complement(2034..2591)
                     /locus_tag="PLG52_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB41753.1"
                     /translation="MSEELVAFFKVLSDANRLKIIGLLAHQPYSVEELAALLGLKPST
                     VSHHLSKLSKVGLVSAKTESYYNVYQLDQKALEEKSRSLFSQDKMTASVADVDVDAYD
                     RKVIGDYTRKDGSLKTIPAQRKKLDAVLRHIVKAFKVGKRYSEKQVNEILSTYHDDTA
                     SLRRELVGAKLMQREGGGGEYWRDE"
     gene            complement(2593..>2936)
                     /locus_tag="PLG52_09660"
     CDS             complement(2593..>2936)
                     /locus_tag="PLG52_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB41754.1"
                     /translation="REYAERVKPAGIYQVKNTVSGKMFLGSSLNLEGPLNRHRFMLKI
                     GSHTNKALQNDWNQLGADAFAFEILEEVKMKDDPNFNLHDELTLLEMIWLEKLQPVEN
                     GYNLNARIREA"
BASE COUNT          735 a          685 c          729 g          787 t
ORIGIN      
        1 cgcctcttct cttggacctg tgtgctttgg cgcttcgatt gaacaaaccc ctcaccgcca
       61 ggctgatgcc cgtcccaggt aaaaaagcgg gagatgcaac cacctttgac ttcgctttct
      121 tcgccaacgg acgtgtgatg gcgttagagt cacagccgct gagcttgcct ttcaatagca
      181 atgagtcatt ttcgctgaag ccaagggcag agagaaaccg tccctgagga aaaggttgaa
      241 gaggggctgc cagttttcgg ttaagaatga agcgggtttt catcctgttt caaatagttc
      301 aagaggaaaa taatcaatga aaagaaatat cctaattatc ggtggattgg tttttgtcgc
      361 aatcgttgcg ttttttgtat ataaagccgt ggcaccatct aagcctgtaa atgtgcaatt
      421 gctggcattg aatgactttc atggaaattt gttgccgcct tctggctctt caggtcaggt
      481 ggacggtgtg gatgcaggcg gcgtagagta tctggcaacg catatcgata cgttgcgcgg
      541 tgaaaaccca aatacggttg tggtttctgc tggtgatctg gtgggagcca gcccgctgct
      601 ttctgcgctc tttcacgatg aaccgaccat tgaagccttt aatttgatcg ggttggattt
      661 caatgccgtt ggcaaccatg agtttgacga aggctgggcg gagttaaaac gtttgcaaga
      721 gggcggttgt caccctgtgg atggatgttt ggatggcgat gatttttcag gagccgagtt
      781 taaattcctt gcggcgaatg ttatcaacaa agagactggc gagccgatct ttccagccta
      841 tgaggtgcgc gagttcgaag gcgtaaaaat cgctttcatt ggcatgacgc tcaaaggaac
      901 gccaagcatc gtcacgcctt ctggtgttgc cgatttggaa tttctggatg aggctgaaac
      961 ggtgaatgca cttatccctg aattgcaagc gcaagggatt gagaccattg tagttttggt
     1021 gcatgagggc gcacgccaaa atggagatgc tcccactgca aatacctgtc aggatattga
     1081 cggaccgatc gtggatattg tcagccgctt tgatgatgcc gtggatgtag tcatcagcgg
     1141 acatacgcat caggcatata actgcttgct ggatggaaaa ctgcttacca gcgccatgtc
     1201 gtttgggcgg ctcgtgacgc aaattcattt gaccattgac cccaagaccg gtgatgtaac
     1261 caccatggaa gccaataatt tggtcgtgac ccgcgatgtg gaaaaggcgt cattactaac
     1321 tgcattgctc gaacgctata acaaagttgc agaaccgctt gccaatcatg aggtgggcac
     1381 tatcaccgca gatattctgc gcgaacccaa tgacgcaggt gaaatggcat taggcgatgt
     1441 gattgcagat tcacagctct ttgccacaag tgcctctgat atgggcaatg ccgtgattgc
     1501 ctttatgaac ccgggcggca tccgcacgga tttggtatat gccgattctg tcggtgaagg
     1561 ggatggcacg gttacttttg gtgagatttt caccgttcag ccgttcaaca ataatctggt
     1621 tacgctcact ctaaccggtg aacagattga tgctttgctt gaacagcaat ttgtagattg
     1681 cgggcagacg gagaatccgc gcattttgca ggtctctgaa ggcttctcgt atacatggaa
     1741 agcgtctggc gctccatgcg aaaaagtgga ccctgctaca atctttttaa atgatgaacc
     1801 aattgacccc gcaaaagaat atcgtgtcac ggtcaactcg tatttagcag atggcggtga
     1861 ccgcttcaaa gtgttgacag aaggaacaga tcgcctcgta ggtgtaatgg accttgatgc
     1921 tttggaagcc tacatgctcc aagcttcacc agtagaaccc ggggcacaag atcgaattaa
     1981 ggttattcca taaacacgaa taaaaagaga ctctggcaag ccagagtctc tttttattcg
     2041 tctcgccaat actctccgcc accgccctca cgctgcatga gctttgctcc caccagttca
     2101 cggcgtaaag aagctgtgtc atcatgatag gtgcttagaa tctcattgac ctgcttttcg
     2161 ctataccgtt tgccaacttt gaaagctttt acgatgtgac gcagaaccgc atccaacttc
     2221 ttacgctgag cagggatggt tttcaagcta ccatctttcc gagtgtaatc accgattact
     2281 ttacgatcat aagcatccac gtccacatcg gcaacagagg cggtcatttt gtcttgagaa
     2341 aacaggctgc gagatttttc ttccagcgcc ttctggtcaa gttgataaac gttgtaataa
     2401 ctttccgtct ttgcgctcac caagccaacc tttgaaagct tggacaagtg atgcgagaca
     2461 gtagaaggtt tcagccccaa taaagcagcg agttcttcca cactatacgg ctgatgcgca
     2521 agcagtccaa tgatcttgag gcgattcgca tccgaaagga ctttgaaaaa ggcgaccaat
     2581 tcttcgctca tatcacgcct cccggattct ggcattcagg ttgtagccgt tttcaacagg
     2641 ctgaagcttc tcaagccaga tcatttccag cagagttaac tcatcgtgaa ggttaaaatt
     2701 cgggtcgtct ttcatcttta cttcttcaag gatttcaaag gcaaaggcat ctgcacccaa
     2761 ttgattccaa tcattttgaa gagccttatt ggtatggctt ccgatcttaa gcatgaagcg
     2821 atggcggttc aacggacctt caaggtttag gctgctgcca agaaacatct taccgcttac
     2881 tgtatttttc acctgataaa ttccagccgg cttaacacgt tctgcatatt cgcgat
//
LOCUS       DAOQNJ010000339         2926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24681_length_2926_cov_7.494253, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000339 DAOQNJ010000000
VERSION     DAOQNJ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24681_length_2926_cov_7.494253"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..764
                     /locus_tag="PLG52_09665"
     CDS             69..764
                     /locus_tag="PLG52_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNB41755.1"
                     /translation="MKTIGLIGGMSWESSIEYYRIINETAKEKLGGLHSAKSLMVTVD
                     FAEIEKLQHEDRWDEAGQILARCAQDLERGGADFIVLCTNTMHKLADQMTSNVSIPLL
                     HIADATAEKIVSAGIKRIGLLGTRFTMEHDFYKGRLIHNYGLDVLIPEKADRDIIHRV
                     IYEELVLGKIVDASRREYQRIMNDLLAKGAEGIILGCTEIELLITEGDSSAPLFPTTK
                     IHAIAAVEKALAE"
     gene            843..1520
                     /locus_tag="PLG52_09670"
     CDS             843..1520
                     /locus_tag="PLG52_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41756.1"
                     /translation="MFNLFKKKEPKQSDPFLDMRTQFMGNVNFNEWPKGDSDAQPWSL
                     FVAARKAYSTNNHAEAEKYLRQVTETPDLEPRHYLQAWLFLRHFLKVQPPAEIAKKVY
                     GVMVEVGTPTGVMAVVGYTDHKARTLHSSGGGTVWERPNASMDDKVDALIKAGENAVQ
                     AIPLVVVDIVPAPPKQADHVLICIATPSGIYHGLGTGDFMWKDPNAGPIVNTGGILLQ
                     ALEALKK"
     gene            1610..2065
                     /locus_tag="PLG52_09675"
     CDS             1610..2065
                     /locus_tag="PLG52_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41757.1"
                     /translation="MRYLNFFLIAVLLSACSTVPTAEPTSTAVPPYRITPDENPYEPK
                     LEDLGRQIDEVTITSVNLAELYDYSPPRTAIGLIGYMPSVCDELRVEISPPDENFNVF
                     IQVYSLVNPDVQCDNVFQQFEARILLGTYSPGRYYVWVNDAPVGDFVSY"
     gene            2113..2574
                     /locus_tag="PLG52_09680"
     CDS             2113..2574
                     /locus_tag="PLG52_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41758.1"
                     /translation="MRRLVITLFLFLFLVSACGAPSPDDPVSNDTVTVHTPAAQETSN
                     YIPSPADGSLTRGEVSLEAKELASLESYPVQFMLQLKGELPTPCHQLRIATNPPDKDH
                     KVLVDVYSVVNPAEICIQVLVPFEIAFPLGSYPEGKYSLWVNGEMVAEFQA"
     gene            complement(2624..>2926)
                     /locus_tag="PLG52_09685"
     CDS             complement(2624..>2926)
                     /locus_tag="PLG52_09685"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNB41759.1"
                     /translation="LTVIPGPRVVDLKLLASFLGEKKMHLPTEREAEQLTGLQAGGIS
                     PLALINKGFQVVIDASAQNFETIYISGGQRGLNIQLGAKDLAGLVNAKFGDVSREG"
BASE COUNT          787 a          694 c          687 g          758 t
ORIGIN      
        1 gaaggaagcg gggtgtacgt catcgcaaaa cacttttttg aaaattcact tttgaatgga
       61 gtacattcat gaaaactatc ggcttgattg gtgggatgag ttgggagtcg tcgattgagt
      121 attaccgcat catcaatgag acggcgaaag aaaagttagg cgggttgcac tcggcaaaga
      181 gtttgatggt aaccgtagat tttgccgaga tcgagaaact gcaacacgaa gatcgctggg
      241 atgaggctgg gcaaattttg gcacgctgcg cgcaagacct tgagcgtggc ggcgcagatt
      301 ttatagtgct atgcacgaac accatgcaca agttagcaga ccagatgact tcaaatgtca
      361 gtattccgtt gctgcacatt gcagatgcaa cggctgaaaa gattgtgtcg gcaggtataa
      421 agcggattgg tctattaggc actcgcttta caatggagca tgatttttat aaaggtcggc
      481 tcatccataa ttacggcttg gatgtgctca ttccagaaaa agcagaccgc gacattattc
      541 accgcgtaat ttatgaagag cttgtgcttg gcaagattgt agatgcatct cggcgtgaat
      601 atcaacgcat tatgaacgat ctgctggcaa aaggcgccga agggattatt ttgggatgca
      661 ccgaaatcga attgcttatt acagaaggtg atagttcagc accgcttttc ccgaccacaa
      721 agatccatgc gattgcggca gtagagaaag cgttggcaga ataaagcgtt ttataaccaa
      781 atgtcctatt taccagcatt gagaaatgca tatactccag caagatcatt tctggagaca
      841 ttatgtttaa cttatttaaa aagaaagaac ccaaacagtc tgacccattt ttggatatgc
      901 gtacccagtt tatggggaat gttaatttca atgaatggcc caaaggcgac tcggacgcac
      961 agccttggtc tttgttcgtt gcggcacgaa aagcatattc gacaaacaat cacgcagaag
     1021 cagaaaaata tctgcgccaa gttaccgaaa ccccagacct tgaaccacgt cactatttgc
     1081 aggcttggtt attcttgcgt cacttcttga aggtgcaacc ccctgccgaa atagccaaaa
     1141 aagtgtatgg tgtcatggtg gaagtcggca ctcccacggg cgtgatggcg gttgtgggat
     1201 atacagacca caaggcacgc accttacact cttctggtgg cggcacggtt tgggaacgcc
     1261 ccaacgcttc gatggatgac aaggtcgatg cccttatcaa agcgggtgaa aatgctgtgc
     1321 aagccattcc gctcgttgtg gtggatattg tccctgcccc acccaaacaa gcggaccatg
     1381 tcttgatctg tattgccact cctagcggaa tttaccacgg acttggcacc ggagatttca
     1441 tgtggaaaga cccgaatgcg ggtcctatcg taaatacggg cggaattctt ttgcaagcct
     1501 tggaagcgct aaaaaaataa ccctacaaaa aactcactca aaccctaccc gagtgagttt
     1561 tttgtatcgc aagaccgtga tattatctgc gtcggagaat cacagtggta tgcgttatct
     1621 taatttcttc ctgattgcgg tcttgctcag tgcttgtagc accgtcccta ctgctgagcc
     1681 gacctcaacg gcagttcccc cttatcgcat cacacccgat gaaaatcctt atgagcccaa
     1741 attagaagac cttggcaggc agattgatga agtcaccatt acttctgtaa atcttgcaga
     1801 gctctatgac tatagcccgc cgcggaccgc cattgggctg atcggttaca tgcccagcgt
     1861 ttgcgatgaa ctgcgagtgg agatcagccc gccggatgaa aacttcaatg tctttatcca
     1921 ggtatatagc ttggtcaacc cagatgtgca gtgcgacaat gtgtttcaac aatttgaggc
     1981 gcggattctg cttggcacat attcccccgg caggtactac gtttgggtaa atgatgcccc
     2041 tgtgggtgat tttgtttcct attaggaacc attcgataca ttttttcgtt aatcaggcag
     2101 gagaaattaa aaatgagacg tttggttatt acattgtttc tatttctatt tcttgtatct
     2161 gcatgtggag ccccatcgcc agatgacccc gttagcaacg atacagtcac cgtccatacg
     2221 ccggcggcac aggaaaccag caactacatc ccaagcccgg cagacggctc tttgacacgt
     2281 ggagaagtct ctctcgaagc aaaagagctt gcctcgttgg aaagttaccc cgttcaattc
     2341 atgttgcaat taaaaggtga actgcccaca ccctgccatc aattacgcat tgcaaccaac
     2401 ccacccgata aagaccacaa agttttggta gatgtgtatt ctgttgtaaa tccggcggag
     2461 atctgcattc aagttctcgt cccatttgag atcgcttttc cattagggag ttaccctgaa
     2521 gggaaatatt ctttatgggt caatggagaa atggtagctg aatttcaagc gtaacaaaaa
     2581 gaggaaagaa gcgcttcagc ttctttcctc ttttgatttt tatttatccc tctcggctta
     2641 catcgccaaa ttttgcgttg accagccctg ccaaatcctt tgcgcccaac tgaatattga
     2701 gtccacgctg accgcctgaa atatagatgg tctcaaaatt ttgagcagag gcatcgatca
     2761 ccacctgaaa ccctttgttg atcaaagcca gcggcgagat tccgcccgct tgcagcccgg
     2821 tcaactgctc ggcttcacgt tcggtaggca aatgcatttt cttttcgccg agaaaactcg
     2881 ccaacaattt caaatccacc acccgcggac cggggatcac cgtcaa
//
LOCUS       DAOQNJ010000340         2915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24849_length_2915_cov_11.018881, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000340 DAOQNJ010000000
VERSION     DAOQNJ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24849_length_2915_cov_11.018881"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..864)
                     /locus_tag="PLG52_09690"
     CDS             complement(76..864)
                     /locus_tag="PLG52_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41760.1"
                     /translation="MALLQTTNVVKRFGGLTAVNKMTFSVDAGKIVSIIGPNGAGKTT
                     FFNSLTGIYKPEEGQILFKDNSLIGLRPDQVAATGIARTFQNIRLFGSMTVIENILVG
                     MHIQLKQGAVDTLFRSKKFNEEEKEAENRAKDLMAYVGLKNVGNELARNLPYGGQRRI
                     EIARALAANPTLLLLDEPTAGMNPNESEDAIKLFRRIRDEKGITILMIEHDMRVVMGI
                     SEHITVMDYGEKIAEGSPQDIRSNQRVIEAYLGRGAAVAHEQKV"
     gene            complement(878..2215)
                     /locus_tag="PLG52_09695"
     CDS             complement(878..2215)
                     /locus_tag="PLG52_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41761.1"
                     /translation="MKTFFSDLYKKLNTGSWPIVVALAFLIVGTITVYLSPRSLTAFL
                     ILMATPVILYFMKMPVKVKIFIGLLLIIVVFPVLGIRNIFYLEVLFQIAVAAALALGL
                     NIVIGMAGLLDLGYVAFYAVGAYAWAIFGSKQMSLLHSIPGAAPANAPFFLDAKWFWL
                     FIFLGVGLAALTGILLGLPVLRVKGDYLAIVTLGFGEVIQVLALNLDKPINLTNGPQG
                     ITPIQRPPLPPDGIMQFFRTILTPLVGHSPTDAEFYNVMFYFLALVVIGIAIVVATRL
                     EDSRIGRAWTAIREDETAAIAMGIPLVKMKLAAFAAGASFAGAMGALYAANRTFVSPE
                     TFDFQVSIGVLTYVILGGLGSIPGVIFGAATVVLLNIQVLQNFSLYLSGLRQSDAVIP
                     IINFAWKNLSNQLDPAKYQKLVFGLILILMMIFRPSGLIPAERRKRELGEEKE"
     gene            complement(2242..>2915)
                     /locus_tag="PLG52_09700"
     CDS             complement(2242..>2915)
                     /locus_tag="PLG52_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNB41762.1"
                     /translation="NAPKLVPLISAIGMSFFLQDLVRLILSLTTGEFNVILPKFGTFD
                     DFWKLAIPGMEDPLRIQIKSVIMVGIAILMLVGLNFLVNGTKVGKAIRAVAQDRPSAS
                     LMGINVNQIISITFLIGGALGGAAGVLFALKATKLDPFSGFFPGLKAFTAAVLGGIGN
                     LTGALLGGLVLGLLESFAASYLSIFTNGAFGAEYKDIFAFAILIVVLVFRPSGILGQT
                     VGQKA"
BASE COUNT          786 a          699 c          802 g          628 t
ORIGIN      
        1 cctttcaagg catggatatg tccgtaatag gaatggacat tgttgagttc aagtagtgtt
       61 cccattcaga atctcctata ccttttgctc gtgtgcaacc gcagcaccac gaccgagata
      121 tgcttcgatc acgcgctggt tcgagcgaat gtcttgcggt gacccttctg caatcttttc
      181 gccgtagtcc atcacggtga tgtgttcaga aatacccatg accacgcgca tatcgtgttc
      241 aatcatgagg atggtgattc ccttttcatc gcggatgcgg cggaagagct tgatcgcatc
      301 ttcagactcg ttcgggttca taccggcggt cggttcatcg agcaggagca gggtggggtt
      361 ggcagcgaga gcgcgagcaa tttcgatgcg gcgctgacca ccatacggca agttgcgcgc
      421 aagttcattt ccaacattct tcaagccgac atatgccatc aaatctttgg cgcggttttc
      481 agcttctttt tcttcctcgt taaatttctt ggagcggaag agagtgtcga cagcgccttg
      541 cttcaactgg atatgcatac cgacgagaat attctcgatc acagtcatgc taccgaacag
      601 acgaatgttc tggaaggtac gggcaatacc cgtcgctgca acctgatctg ggcgcaagcc
      661 gatcaaagaa ttgtctttga aaaggatctg cccttcttca ggtttgtaga taccggtgag
      721 gctgttgaag aaggtggtct ttccggcgcc gttcggtccg ataatgctga cgatctttcc
      781 cgcatccacc gagaaggtca ttttgttgac ggctgtcaga ccgccgaagc gttttactac
      841 attggtggtt tgtaagagag ccatggtagg aatccgccta ttctttctct tcgccaagtt
      901 cgcgtttgcg gcgctcggct ggaatcaagc cagagggtct aaagatcatc attaagatga
      961 ggatcaaacc aaatactagc ttttgatatt ttgccgggtc caactgattg gagaggttct
     1021 tccaagcgaa gttgatgatc ggaatgactg catcgctttg gcgaagtccg ctcaggtaga
     1081 gggagaagtt ttggagcacc tgaatgttga ggagcacaac ggttgcggca ccgaaaatca
     1141 cgccggggat actgcccaaa ccaccgagga tgacgtaggt caacacgccg atggaaactt
     1201 ggaagtcgaa ggtttcaggg ctgacgaagg tgcggttggc agcgtagaga gcgcccatgg
     1261 caccagcaaa agaggcaccg gcggcgaaag cggcgagctt catcttcaca agcggaatgc
     1321 ccatagcgat ggctgcggtc tcatcttcgc gaatggctgt ccaagcgcgt ccaatgcggg
     1381 agtcttcgag gcgggttgct acaacgattg caatcccaat gacgacgagc gccaggaagt
     1441 aaaacatcac attgtagaac tcagcatctg taggggagtg ccctaccagt ggggtgagaa
     1501 tcgtgcggaa gaactgcata attccatctg gtggcagggg cgggcgttgg atgggggtaa
     1561 tgccttgcgg accattggtc aggttgatgg gcttatcgag gttgagcgcc aatacttgga
     1621 tcacttcacc gaagccaagt gtaacgatgg caaggtaatc accttttacg cgcagaacgg
     1681 ggagacccaa caagatgccg gttagagcag ctaaacctac gcccaagaaa atgaatagcc
     1741 aaaaccattt tgcgtcgagg aagaagggag cattggcagg agccgcaccg gggatcgagt
     1801 gcaacaaaga catttgcttt gaaccaaaga tcgcccaggc ataagcgccc actgcgtaga
     1861 acgccacata cccaaggtct aacaagcctg ccataccgat cacgatgttc aacccaagag
     1921 caagtgcggc tgcaacagcg atttgaaaga gaacttccaa atagaagatg ttacgaatgc
     1981 ctaataccgg gaagaccaca ataatcaaaa gcaacccaat gaagatcttt actttcactg
     2041 gcattttcat aaagtaaaga atgacagggg tagccatcaa gatcaagaag gctgtgaggg
     2101 aacgtgggct aagataaaca gtgatcgttc ctacaatgag aaaggcaagt gcgaccacga
     2161 ttggccacga gcccgtattg agtttcttgt acaagtcgct gaaaaatgtt ttcatggttg
     2221 tgtacctttc ctgaatacgg actaagcctt ctgcccaacg gtctgaccga gaataccgga
     2281 ggggcggaag accaacacaa cgatcaggat ggcaaacgcg aaaatatcct tgtactcagc
     2341 gccgaaagca ccattcgtaa agatcgaaag gtaggaagcc gcaaaagact cgagcaagcc
     2401 aagcaccaat ccacccaaga gggcgccagt gaggttgccg ataccaccaa gaacggctgc
     2461 cgtaaatgct ttcaaaccgg ggaagaagcc agagaaggga tcaagtttcg tagccttaag
     2521 ggcgaacaat acaccagccg caccacccag agcaccaccg atgaggaagg tgatcgaaat
     2581 gatctggttg acgttgatgc ccatcaagct agcggaagga cgatcctgcg caacggcgcg
     2641 gatggcttta ccaaccttag tgccgttgac gaggaagttc aagccaacca acatgaggat
     2701 ggcgatgcct accatgatga cagacttgat ttgaatgcgt aggggatctt ccatgcccgg
     2761 aattgccagc ttccagaagt cgtcgaacgt accgaatttg gggaggatga cgttgaactc
     2821 gcccgtggtg aggctgagaa tcaaacgtac aaggtcctgc aggaagaagg acataccgat
     2881 ggcagagata agcggaacca actttggagc gttgc
//
LOCUS       DAOQNJ010000341         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24875_length_2914_cov_9.254285, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000341 DAOQNJ010000000
VERSION     DAOQNJ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24875_length_2914_cov_9.254285"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PLG52_09705"
     CDS             complement(<1..869)
                     /locus_tag="PLG52_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB41763.1"
                     /translation="MSAATRSSKDWLQLASLGEQLRSEDSLSAQRDRIAAMANRLIEG
                     KVDVWLHENIFRLPDWDDGYIFPTQPALDGMKRAIKTGKLARKKKSSGKKTPASRSTF
                     AAIPLEDQGITLGALQVSRSKGPEFTDDELNLLQGLGHIISVNLFASHRAEVEQFRLR
                     QLNLVREVSAQIANVLDVDDLASQVTRLIQKTFNFYYVAFFTLDPQDNALHFRASAVA
                     PRKGRRKASIALEVEIGQGLIGEAAKTGMEIVCDDVQSDSRYRFIDSLPETRSEVVIP
                     LKIEDHILGALDV"
     gene            complement(874..1281)
                     /locus_tag="PLG52_09710"
     CDS             complement(874..1281)
                     /locus_tag="PLG52_09710"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB41764.1"
                     /translation="MPAYTFPAKFEFLDEIRDTVAEIARSGGFSEKTIYSLQLAADEA
                     ASNIIEHAYEGVSNATLYMTCDMKGDEIVITMRDTGKSFNPTTVKEPNLKADLSERQI
                     GGLGVYLMRKLMNTVHYESSKSGNLLTMTKRRE"
     gene            complement(1351..1686)
                     /locus_tag="PLG52_09715"
     CDS             complement(1351..1686)
                     /locus_tag="PLG52_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB41765.1"
                     /translation="MEITTQEFKHCDLIAVKGRVDSATAPQFAKALETANDNGRFKLA
                     LDMSELEYMSSAGFRALLAAQRNCKRYNRGEVVLVRVPDRIREALELAGFTELFKTFD
                     DTVTAVGSF"
     gene            1797..2741
                     /locus_tag="PLG52_09720"
     CDS             1797..2741
                     /locus_tag="PLG52_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HNB41766.1"
                     /translation="MRKGLLLYNPAAGRFPVRRHVRGIIKPLNDAGWSMDIAETLSGT
                     HAVQTAHQAAQEKYDAVFAIGGDGTVGQVASGLIHTETALGVLPAGTVNVWALEQGQK
                     PYSWWQPGNLKENAKLLSNVEPQYVDVGDCNDQPFLLWAGVGLDAHTINKIEPRPRIF
                     KHIAFPHYFVTTVWEATFWHGLDLRIYAEGKLVEGHYLVAVATNIRHYAGGQSLISPN
                     ALLDDNEMDLWLLSGSNVADALRHYFDLVAGRHLTSEEARCIPFHHVTIESDANFSIQ
                     VDGDPMLGGHKAEIKVKHRALKVLVPENALYLLKTPQR"
BASE COUNT          701 a          738 c          716 g          759 t
ORIGIN      
        1 tgaacatcca atgcgcccag aatatggtct tctatcttga gcggaatgac aacttcagag
       61 cgagtttctg ggaggctatc aataaagcga taacgagaat ccgactgaac atcgtcacag
      121 acgatctcca tgccggtctt tgctgcttca ccgatcaacc cctgaccgat ctccacttcc
      181 aatgcaatgg atgctttacg gcgaccttta cgcggtgcaa cagcgctagc acgaaagtga
      241 agcgcattat cttgagggtc gagagtgaaa aaggcaacgt aataaaagtt aaaggtcttt
      301 tggataagtc ttgttacttg agatgctaga tcgtccacat cgagcacgtt ggcgatctga
      361 gcgctgacct cgcgtaccaa gttcaattgt cgcaagcgga attgttccac ttcggcgcgg
      421 tgcgaggcaa ataaatttac agagatgata tgccccaagc cttgaagcag gtttaattca
      481 tcatcagtaa actctggtcc ttttgagcgg gatacttgaa gcgctcccag cgtaatcccc
      541 tgatcttcga gggggattgc agcaaaggtc gagcgagagg cgggggtttt cttcccgctg
      601 ctttttttct tacgagccag tttccccgtt tttatggcac gtttcattcc atctaatgca
      661 ggctgggttg ggaagatata tccatcgtcc caatctggca ggcggaagat gttttcgtgc
      721 agccagacat ccaccttgcc ttcgatgagg cggtttgcca tagcagcgat gcgatctcgt
      781 tgcgcgctaa gagagtcttc gctccgcagt tgctcaccca agctggcgag ttgaagccaa
      841 tccttagagg atcgggttgc ggcgctcatg aaactattct ctccgtttgg tcattgtcag
      901 tagattgcca gatttggaag attcgtaatg gacggtgttc atcaacttcc gcatgagata
      961 tacgcccagc ccgccgatct gccgttctga taggtctgct ttgaggttcg gctctttgac
     1021 cgtggtaggg ttgaatgact ttccagtatc tcgcatggtg atgacgatct cgtccccttt
     1081 catatcacac gtcatataca aagtagcgtt cgacacacct tcataggcat gttcaatgat
     1141 gttagaagca gcttcgtccg cggcaagttg gagggagtaa atggtctttt cagagaaccc
     1201 tccgctgcgc gcaatctctg caactgtatc gcggatctcg tccagaaatt caaacttggc
     1261 tgggaatgtg tatgctggca taaagaaaat gagatgccgt cccttgtctt taaagagacg
     1321 gcatgtcggt tctttttagg aacccttcta ttagaaactt ccgacggcgg ttacggtgtc
     1381 gtcgaaggtt ttgaagagtt cggtgaagcc tgccaattcg agggcttcgc gaattcggtc
     1441 tggaacacga acaagcacca cctcgccgcg attgtatcgt ttgcagttgc gttgtgctgc
     1501 gagcaaggca cggaaacctg cgctggacat gtattccaat tcgctcatat caagggcaag
     1561 tttgaaacgt ccattatcgt tggcagtttc aagtgccttg gcaaattggg gagctgtcgc
     1621 gctgtcaacc cgtcctttta ctgcaatcag gtcacagtgc ttgaattctt gggtcgtgat
     1681 ttccataatt tctcctccga acaagatatc gagtgatgaa ttagcgggat tatatcacgt
     1741 agataggtga attttttata tatcctccca tcccccatcg gctataatcc ccacccatgc
     1801 gcaagggact cttactctac aaccccgccg cggggcgttt tccggttcgc cgccatgtgc
     1861 gtggcatcat caaacccctt aatgatgccg gttggagcat ggatattgct gaaacactca
     1921 gcggaaccca tgctgtacaa accgcgcacc aagctgcaca agaaaaatat gatgcggtct
     1981 ttgccattgg tggcgatggc accgtagggc aagttgccag cggtttgatc cacaccgaaa
     2041 ccgcgctggg cgtactccct gctggaaccg taaatgtttg ggcattagag caagggcaaa
     2101 agccctatag ttggtggcag cctggcaatt taaaagagaa cgcaaagtta ctgtcaaatg
     2161 ttgaaccgca atatgtagat gtaggcgatt gcaacgatca gccttttcta ttgtgggcag
     2221 gcgttggctt agacgcacac accatcaaca agatcgagcc acgtccgcgc attttcaagc
     2281 atattgcatt tccccattat ttcgttacca ccgtctggga agccaccttc tggcatgggt
     2341 tagacctacg aatttatgcc gaaggaaagt tagtggaagg gcattatcta gttgcggtag
     2401 ccaccaacat tcggcactat gcgggcggac aatctcttat ctctcccaat gctctgctcg
     2461 atgataatga aatggactta tggctattaa gcggcagcaa tgttgccgat gccttgcgtc
     2521 actactttga ccttgtagcc ggcagacatc tcacctctga agaagcacgc tgcatcccgt
     2581 tccatcatgt caccattgag tctgatgcga acttctccat tcaagtagat ggcgacccca
     2641 tgcttggcgg gcacaaagca gagatcaaag taaagcaccg ggcgctcaaa gtgctagtac
     2701 ccgaaaacgc tttatacttg ctgaaaacac cgcagagata atatggactt aaaaccctat
     2761 ctaaatcgct tcgtcattgt gggatcattg attactgccg ggattttgat tctgatcacg
     2821 ctcattctca tcggttggac gtcgccccgc ttctctcccg aagttgggtt tgctccagca
     2881 gacctaacca tgatccccgc cccaacccac accc
//
LOCUS       DAOQNJ010000342         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_24985_length_2906_cov_9.003157, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000342 DAOQNJ010000000
VERSION     DAOQNJ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24985_length_2906_cov_9.003157"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PLG52_09725"
     CDS             complement(<1..1073)
                     /locus_tag="PLG52_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41767.1"
                     /translation="MRKRIIPIFIVILALAGIVYVWNNSNQPAEPQATPTEEAFIVPT
                     VVSAHAPTVLPLANSPLKSSITSAGDYLVRQQLANGELAYQVNLLTNDRASTPSYIRL
                     MGGTSALYTVCRVAEDTVYCQAADRALEHYLPNLLTDPDHFKGTCLYSNGVCPLGGAT
                     VTIDAIYKRWQATNSVILNDHDLLATAVDLGYFIVSMRRPEGGFYHSFDPHFAGTVDP
                     SYVDPSFNGEAVAALLQLYEMTNNEFWLEQAHDVNTFMLTQPVTEDYAHSYAFALFAR
                     LDELSQADQDYAKQIADLIIAGQIRSLNPANSSVATATKIEALSSIAQALTLSNNKPE
                     GLDREIITFVTFVQARQFPANDC"
     gene            complement(1088..1747)
                     /locus_tag="PLG52_09730"
     CDS             complement(1088..1747)
                     /locus_tag="PLG52_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNB41768.1"
                     /translation="MLKALIFDFDGLILDTETPEVLVWQDIYKEHGFELPVEEWEKTV
                     GGYGISSFDPAHHLSHLSQGRLDPVSLKARYRLEADAIIHTKPIQPGIMDMIGSAKEN
                     GLKVAIGSSSPHSWVDTHTQRLNIYDQFDHIICQDDVAPGRTKPHPDIYLKALETLQV
                     DKAEAVVFEDSPNGVLASKRAGVFVVAVPNPLTARMNVQGDLTVSSLADLTLQDLINR
                     H"
     gene            complement(1749..2069)
                     /locus_tag="PLG52_09735"
     CDS             complement(1749..2069)
                     /locus_tag="PLG52_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41769.1"
                     /translation="MSSWSIYNKGSTIGTLSAEGGLILSDEAHDGGARITLKRGTNYV
                     SVSLHILGWMDHTRFFANDADAQREYRNMRSGVLTVLDVINTEGVSDIKIWEAISDFV
                     RRFP"
     gene            complement(2062..>2906)
                     /locus_tag="PLG52_09740"
     CDS             complement(2062..>2906)
                     /locus_tag="PLG52_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41770.1"
                     /translation="STRALTRGHPLGAMGLLAYINPARHLYAAVSDSDDVTLLGGIIH
                     TRGEPYAKLLYLAPSQQLNKPQLTALIEHLTAQAGVWQAFHVIAEVDETSEVFIALRK
                     AGFSVYAWQRVWDASLIQKTESSPSTGWRRVKSIDLPAIQRMHYQIVPQLLHLVDPAP
                     KLPNGFIHTDGYCFASVNYGVYGVVLTPWILPDEGDVSEKIVSLIASLPDRRGRPVYL
                     NVRSYQAWLEPVLEDLGAKAAPRQAVMVKYLSRLVKEGVPARVVPSNARIQPTQMSRM
                     DTDE"
BASE COUNT          758 a          724 c          710 g          714 t
ORIGIN      
        1 ttacaatcat tggcagggaa ttggcgtgct tgcacaaaag tgacaaaggt gatgatctca
       61 cggtccaacc cttcaggctt gttattagac aaagtcagcg cctgagcaat ggacgataag
      121 gcttcgatct tggtggcggt tgcaacgctg gagtttgcgg ggttcaacga gcggatctgc
      181 ccggcaatga tgaggtctgc gatctgtttg gcataatctt ggtctgcctg gctaagttca
      241 tctaaacggg cgaataaagc aaatgcataa ctgtgagcgt aatcttctgt cacaggttgg
      301 gtgagcatga atgtattcac gtcatgagct tgctcaagcc aaaactcgtt gtttgtcatt
      361 tcataaagct gtaacagagc cgcaaccgct tcgccattga atgaggggtc cacataactg
      421 gggtccactg ttccggcgaa gtgcgggtca aaggagtgat agaagccgcc ctcgggtcgg
      481 cgcatggaga cgatgaaata accaaggtct acagccgtag cgagcaggtc atgatcattg
      541 aggataacgc tgtttgtggc ttgccaacgt ttgtagatgg catcaatggt aaccgtcgcc
      601 ccgcccaacg gacaaactcc attcgaataa agacatgtcc ctttgaaatg gtctgggtct
      661 gttaagaggt tggggagata atgttccaag gctcggtctg cagcttggca atagactgta
      721 tcctctgcta cacggcagac cgtgtacaag gcactggttc cacccatcaa gcggatatac
      781 gacggtgtag aggcgcggtc attcgtaagc aaattaactt gatacgccaa ctcaccattc
      841 gccaattgct gacgcacaag ataatctcct gccgaagtga ttgatgattt cagcgggctg
      901 ttcgccaatg gaagaactgt cggcgcatgt gcggaaacaa ccgtaggcac gataaacgct
      961 tcttcagtgg gtgtcgcctg cggttctgca ggttgattgg agttgttcca aacgtagaca
     1021 atcccagcca aagcaaggat cacaataaaa atgggaatga ttcgtttccg cataaatcga
     1081 caatcaacta gtgtctattg attaagtcct gcaaggttag gtctgccaaa gaagaaacgg
     1141 tcaggtcgcc ttgcacgttc atccttgctg tcaatgggtt tggaacagcc accacaaaaa
     1201 ctccggcgcg tttagatgcc aagactccat tcggtgaatc ttcaaagacc accgcttcgg
     1261 ctttatctac ttgcaaggtt tccagagctt tcaaataaat atcggggtga ggtttggtgc
     1321 gtccgggagc tacatcatcc tgacagatga tgtgatcgaa ctggtcgtag atgtttaatc
     1381 gctgtgtatg agtatctacc caagaatgtg gagaactaga gccaatggca actttcaagc
     1441 cgttctcttt tgccgagccg atcatatcca tgatgcccgg ttggattggc ttggtatgaa
     1501 tgatcgcatc cgcttccagc ctgtagcgag ctttgaggga aacaggatcc agccgtccct
     1561 gagaaagatg cgaaagatgg tgggcggggt caaagcttga aatgccgtat ccgcccacgg
     1621 ttttttccca ttcttccacg ggaagttcaa atccatgttc tttgtaaatg tcctgccaaa
     1681 ctaaaacttc gggtgtttcg gtatcgagaa tcaacccgtc gaaatcaaat atgagtgctt
     1741 tcaacatatt atggaaaccg cctgacaaaa tctgaaattg cttcccaaat cttgatatcg
     1801 cttacacctt ccgtgttgat cacatccaac acggtgagaa cgcccgaacg catgttgcga
     1861 tattcacgtt gcgcatcggc atcattagca aaaaatcggg tgtgatccat ccaaccaaga
     1921 atgtgaagtg aaactgaaac ataattcgta ccacgcttaa gcgtaatccg tgcgccacca
     1981 tcatgcgctt catctgaaag aatcaaacca ccttccgcac taagtgtgcc aatggtgcta
     2041 cctttgttat aaatcgacca actactcatc cgtatccatt ctactcattt gggtgggttg
     2101 aatgcgcgca ttcgagggca ccacacgagc tggcacaccc tctttaacca agcgcgataa
     2161 atatttcacc attacggctt gccgcggcgc agcctttgca ccaaggtctt ccaacacagg
     2221 ctcaagccaa gcctgatacg atctcacatt caaatacacc gggcgcccgc gccgatctgg
     2281 caggcttgcg atcagtgaaa caattttttc gctcacatcg ccttcatccg gaagaatcca
     2341 cggggtgagc accacgccat acacaccata attgacgctc gcaaaacaat agccatcggt
     2401 gtgaataaat ccattcggaa gctttggagc cgggtcaacc agatgcaata actgcggaac
     2461 gatctgataa tgcatgcgtt gaatagcggg cagatcgatg gattttacac gtctccatcc
     2521 cgtacttggg cttgattctg ttttctggat caaactcgca tcccaaaccc gttgccatgc
     2581 atagacggag aaacctgcct tccgtagtgc aatgaaaacc tcgctagtct catccacctc
     2641 ggctatgaca tgaaatgcct gccatacacc cgcctgcgcc gtgagatgtt cgattaacgc
     2701 tgtaagttgt ggtttattaa gctgttgaga cggtgcaaga tataaaagct ttgcataagg
     2761 ttccccgcgt gtgtgaatga tgccgcccaa caaggtcaca tcatcgctat cactcaccgc
     2821 tgcatacaaa tgccgcgccg ggttgatgta tgccaacaat cccatcgcac cgaggggatg
     2881 tccgcgtgtg agggcacgcg tgctat
//
LOCUS       DAOQNJ010000343         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25016_length_2904_cov_9.619165, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000343 DAOQNJ010000000
VERSION     DAOQNJ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25016_length_2904_cov_9.619165"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1050)
                     /locus_tag="PLG52_09745"
     CDS             complement(265..1050)
                     /locus_tag="PLG52_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41771.1"
                     /translation="MMRTKAIYLILVLLCFSFACQALFPATPPPRDGVVIDNCSEFVK
                     SIRSMQTNDTPQELIETGIKQGGEFDVNEYFNVTPNLSMQEGYALDYVIIPDSLGSFP
                     LLAARPVDQPPYKSSSEVSVDSELTNYWKYMDVNDNEQGYFEYASFLLLANQFYLVWH
                     ANYNDLGIVCDRATVDNIVANVNDGGFGIEFDREQMKQIKALQNIEPLVKLTDTTATV
                     ELVVFSNWGGFYRMTYTISRSSPHEIMDRQDENIIPYDCGIMF"
     gene            complement(1112..1474)
                     /locus_tag="PLG52_09750"
     CDS             complement(1112..1474)
                     /locus_tag="PLG52_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41772.1"
                     /translation="MSHTCEAIVVHCMDYRLQSYINQWLETNLGKGSYDRAVMAGGVY
                     DVYDVIRQVDISARLHGITKVVLINHEDCGMYGPGGTEERHEEDLDKAEDKIRRLFPH
                     LEVDSYYLNLDGTFERKS"
     gene            complement(1491..2528)
                     /locus_tag="PLG52_09755"
     CDS             complement(1491..2528)
                     /locus_tag="PLG52_09755"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNB41773.1"
                     /translation="MTQFLTLSPHSPSPELIRQAADVILRRGLVAFPTETVYGLGANA
                     LDEEAVAKIFHAKGRPSNDPLIVHVASLDDVKLVAAEIPPIVEKLAAAFWPGPLTLVL
                     PKQAHVPASVTAGLDSVAVRVPSHPVALALIRASGVPIAAPSANRFGHTSPTTAQHVI
                     DDLNGRIDMILDGGATSVGVESTVLDVTRDPAMLLRPGGVSREAIEKIIGVVELRNNR
                     PNSDDEAQVSPGLLGKHYTPNAELILCKGERDSALEKLLGLAKQAQAEGRSVGLLLAN
                     EDMASFESACIPYPLGASNDLEALARNLYAGMRALDDQGVKLILTRDFGKQGLGLAIH
                     DRLLRAASQII"
     gene            complement(2528..>2904)
                     /locus_tag="PLG52_09760"
     CDS             complement(2528..>2904)
                     /locus_tag="PLG52_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41774.1"
                     /translation="PTKKPIIHCDALVVHCMDYRLQKFIQPWITVRFGYDNFDIISLA
                     GSVHDYEMVLKYVQLAVQIHTIETVCLINHEDCRAYGRDGTYKRHKHDLTDAQKKIKA
                     LFPHLKVETFYLHLDGTFEPIS"
BASE COUNT          758 a          711 c          678 g          757 t
ORIGIN      
        1 accagcgcaa catttagacc tgcaacgagg tcattgcgaa atgtggcgag cgaatagccc
       61 gcaaactcgt ccctgtaaag acgggcaagg gaacgtcttg gcatatttaa ttctccgtaa
      121 atagtagtac accaaaggct cggtgctccc ccaaggcgtc ctttggttta tgtcgggcgg
      181 tattttatca ctgctgattt tattttgtat acggatttaa tagactacac ggtattcgta
      241 atgaatccgt gtagtctatt tattctagaa cataatccca caatcatacg gaataatatt
      301 ctcatcttgt ctatccataa tctcatgcgg agacgatctg ctaatcgtat aggtcatgcg
      361 ataaaagcca ccccaattgc taaacacaac caattctacg gtggcagtcg tgtccgtcag
      421 cttgacgagt ggttcgatat tttgcagagc cttgatctgt ttcatttgtt ctctatcgaa
      481 ctcgatgccg aaccctccgt cgttgacgtt tgcaacaata ttatcaacgg ttgcacgatc
      541 gcagactata cccaagtcgt tgtaattggc atgccaaact aggtagaatt gattggcaag
      601 aagcaggaag gaagcgtatt caaaatatcc ctgctcgtta tcgttgacgt ccatatactt
      661 ccaataattt gtgagttcag aatccacgga gacttcgctc gatgatttat atggtggttg
      721 atctaccgga cgagcagcca aaagcggaaa ggaacctaat gaatcgggga taatgacata
      781 atccagcgca tatccctctt gcatagaaag gtttggtgtg acgttgaagt attcattcac
      841 atcaaattca cccccttgtt tgatgcccgt ttcgatcaac tcttgaggcg tatcgtttgt
      901 ctgcatactg cgaatcgact taacgaactc tgaacagttg tcgatcacaa ccccatcacg
      961 gggtggcggt gtagcaggaa agagcgcctg acacgcaaaa gaaaagcaca acaaaacaag
     1021 aattagataa attgcctttg ttctcatcat atgctccaaa taaaagggtt gagaaaaaga
     1081 cgaaagccgt tctgaaaatg ttccttattg gctaagactt acgctcgaag gttccatcta
     1141 agtttaggta atatgaatct acttcaaggt gtgggaataa cctgcgaatt ttatcttcgg
     1201 ctttatcaag gtcttcttca tggcgttctt ccgtcccacc aggaccatac ataccgcaat
     1261 cttcatgatt gatcagcacc acctttgtaa taccatgcaa ccttgctgaa atatccacct
     1321 gacgaatcac atcataaaca tcgtacacgc cacccgccat cacggcgcgg tcgtaactcc
     1381 ctttgcctaa attcgtttcc aaccattggt tgatataaga ctgtaaacga taatccatgc
     1441 aatgaaccac aattgcttca cacgtatgag acatttcttc cctccgaaaa ttaaatgatc
     1501 tgactcgctg ctcttaacaa gcggtcatga atagccagcc ccaaaccttg ttttccaaaa
     1561 tcacgtgtca gaatcaactt cacaccttga tcgtccaacg ctcgcatgcc cgcataaaga
     1621 ttccgcgcaa gcgcttcgag gtcattggat gcaccaagcg gatacggaat gcaagcggac
     1681 tcgaaagacg ccatgtcttc atttgcaaga agaagtccca cgcttcttcc ttccgcttgt
     1741 gcctgtttcg ccaatcccag cagtttttcc aacgctgaat ccctttcgcc tttgcatagg
     1801 atcaattctg catttggcgt gtaatgtttc cccaataatc ccggcgagac ttgcgcctcg
     1861 tcatccgagt tggggcggtt atttcgtaac tcaactacac caatgatttt ctcgattgcc
     1921 tcgcgcgaaa ccccgcccgg gcgtagaagc attgccgggt cacgggtcac atccagcacc
     1981 gtcgattcga ctcccactga agttgcaccg ccatcgagga tcatgtcaat gcgaccgttg
     2041 agatcatcta tcacatgttg agccgtggtc gggctggtat gcccgaagcg atttgcagat
     2101 ggcgcagcaa taggcacacc actcgcacgg atgagcgcca acgccacagg gtgtgacggg
     2161 acacgtactg caactgaatc taaccctgct gtgacagatg cggggacatg cgcttgcttt
     2221 ggcagcacca acgtcagcgg accgggccag aaagcggcgg cgagcttttc aacgatgggg
     2281 gggatctcag cggcaacgag ttttacatca tcaagcgaag ccacatgcac aatcagcggg
     2341 tcattggaag ggcgcccttt ggcatggaaa atttttgcca cagcttcctc atcgagagcg
     2401 ttggctccaa gtccgtatac ggtttcggtg gggaaggcaa ccaagccgcg gcgtaggatc
     2461 acgtctgcgg cttggcgaat cagttcaggg cttggggaat gaggcgaaag ggtcaggaat
     2521 tgggtcatca agagattggt tcgaaggttc catccaagtg cagatagaac gtctcaactt
     2581 tcaagtgtgg aaataaagct ttgatttttt tctgagcatc ggtcaggtca tgtttgtggc
     2641 gtttatatgt gccgtctcgt ccataggcgc ggcaatcttc gtggttaatc agacagacgg
     2701 tttcaatggt atggatttgc acagcaagtt ggacatactt caataccatt tcatagtcat
     2761 gtacactacc tgccagtgag atgatgtcga agttgtcgta tccaaagcgg acggtgatcc
     2821 acggttggat gaatttctgc aggcggtagt ccatgcagtg aacaacgagt gcgtcgcagt
     2881 gaatgatcgg ttttttggtt ggca
//
LOCUS       DAOQNJ010000344         2900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25075_length_2900_cov_6.669244, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000344 DAOQNJ010000000
VERSION     DAOQNJ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25075_length_2900_cov_6.669244"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1089
                     /locus_tag="PLG52_09765"
     CDS             61..1089
                     /locus_tag="PLG52_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNB41775.1"
                     /translation="MLSRLKSAARILIKGEPKKKTRSAIPAITAEELVEVKQFFPREK
                     FFVMGHARSGTTLLMRLLRLHEEVHCNYQAHFFSRKPMLKSLVDSPDAEEWLSRKSNR
                     WNQGRDLSPLVLRATADFIMERDAAREGKHIVGDKSPSSTIHGQIVRDTHLIYPDAKI
                     IYIVRDGRDVLTSERFRNFVEESKFLTSEDKRIMADLRTDSAPFSDGRRSIFTETSIR
                     NIAKRWVDDLNEIDEESKRLYGENYISLRYEDMLSNPFAELSRLWKFLDVKKVDSALE
                     KQLVEEMSSNPDEEWQAKRNEGIASFLPKGQAGNWQRLFTARDKALFKEIAGDVLTKW
                     GYEKDLNW"
     gene            1159..2151
                     /locus_tag="PLG52_09770"
     CDS             1159..2151
                     /locus_tag="PLG52_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB41776.1"
                     /translation="MKYLIAGLGSVGRRHMRNLVALGETDIVLYRTHKATMPEDELAG
                     YPVETDLSEALKKHKPDAVIVSNPTSLHLDIAIPAAEAGCAILLEKPIAGSMERVDVL
                     QKTVKKSGSKVLVAFQFRFHPGMVKTRELIENGEIGRVVSASVHFGEYLPAWHPWEDY
                     RQGYAARADMGGGVVATQCHSLDYLPWLVGKKVESVWGFADKLSDLEVTADDTAKIGL
                     RFEGGAVGSLHLDYNQQPPEHEFRIIGTNGTIKWNLVDGAARIYRTEKKDWDVYPLPE
                     GWERNVMFQQQTKHFVDIVNGKAQPSCTLEDGVQVQRIIASVHESQETGKLIKL"
     gene            2390..>2900
                     /locus_tag="PLG52_09775"
     CDS             2390..>2900
                     /locus_tag="PLG52_09775"
                     /inference="COORDINATES: protein motif:HMM:NF025664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41777.1"
                     /translation="MAKANLITPYGGKLVDLVVKGAERDALIARAGHLPSIKITMRNL
                     CDLELIATGGFSPLTTFMGKADYERVLKEMRLADGTLFPLPITLTADPKELPTVGEEL
                     ALRNANFDLIAVMRLDEVYHWDANTEAALAYGSTDTKHPMVSEMGRWGKVCISGPLQV
                     INLPKYYDFV"
BASE COUNT          743 a          623 c          784 g          750 t
ORIGIN      
        1 attgccggga ttcttgtgtg cctggcgggg ttggtcatgt taaattggaa gagataattc
       61 atgctttctc gtttaaagtc tgctgcccgt attttgatca aaggtgaacc caaaaagaaa
      121 acgcgtagtg ccattcctgc aattaccgct gaagaacttg tagaggttaa gcaattcttc
      181 ccacgtgaaa aattctttgt gatgggtcat gcccgcagtg gaacgacctt gcttatgcgt
      241 ttgctgcgtt tgcatgaaga agtccattgc aattaccaag cacatttttt ttcacggaag
      301 cccatgctca aatctttggt ggattcgcca gatgcggaag aatggctctc acgcaaatca
      361 aaccgctgga atcaggggcg tgacctttcg ccgttggttt tacgtgccac ggctgacttc
      421 atcatggaac gtgatgctgc tcgcgaaggc aaacatatcg tgggcgataa aagtccatca
      481 tctaccattc acggacagat cgttcgtgac acccatctga tctaccccga tgccaagatt
      541 atctatattg ttcgtgatgg tcgggatgta ttgacctcgg aacgcttccg gaattttgtt
      601 gaagaatcca agtttctcac atctgaagat aaacgcatca tggcggacct gcgaactgac
      661 tctgctccat tcagcgacgg acgccgctcg atctttaccg agacgtccat tcggaatatt
      721 gccaagcgct gggtggatga tctgaacgag attgacgaag aaagcaagcg tttgtacggt
      781 gagaattaca tctcattgcg ttatgaagac atgctttcta acccctttgc tgaactttcc
      841 cgtttatgga agttccttga cgtaaagaaa gtagattcgg ctttagaaaa acaacttgtt
      901 gaagagatgt cctctaaccc agatgaagaa tggcaagcaa agcgcaatga aggtatcgcc
      961 tctttcttgc caaaaggtca ggctggcaat tggcaacgct tgttcacagc acgtgataaa
     1021 gccttgttca aggaaattgc aggggatgta ctaacgaagt ggggatatga gaaagatttg
     1081 aattggtaaa ttcataaggg cggggcttgt gccacaacgt ggtatttccc cgcccttatt
     1141 cgtatcaagg atggaaacat gaaataccta attgcaggac ttggctctgt tggacgccgc
     1201 catatgcgca acttggttgc gctcggagaa acggatattg tcctctaccg cacccacaaa
     1261 gcaaccatgc ctgaggatga actggcaggc tatcccgttg agacagactt gtctgaagcc
     1321 ttgaaaaagc ataagccaga tgcggtcatc gtctcgaacc ccacttcatt gcatttggat
     1381 attgctattc ctgccgcgga ggcgggctgt gctattttgc tggagaaacc cattgccggc
     1441 tcaatggagc gcgtggatgt tttgcaaaag actgtgaaaa aaagcggctc gaaggtgttg
     1501 gtggcgtttc agttccgctt tcacccgggt atggttaaga cccgtgaact gattgaaaac
     1561 ggtgagattg ggcgggttgt atcggcaagc gtgcatttcg gtgagtattt gccagcttgg
     1621 cacccgtggg aagattatcg tcagggatat gcggcgcggg ctgatatggg tggaggcgtg
     1681 gttgcaacac aatgtcactc attggattat ttgccgtggt tggttggaaa gaaggtggag
     1741 tctgtttggg gctttgctga caaacttagc gaccttgaag tgactgcgga tgacacagcc
     1801 aagatcgggc tgcgatttga aggtggtgca gtaggaagcc ttcacctaga ctataaccaa
     1861 cagccgccag agcatgaatt ccgcattatt ggcacgaatg gaaccattaa gtggaatttg
     1921 gtggatggag cggcgagaat ttaccgcaca gagaaaaaag attgggatgt gtacccgcta
     1981 ccggaggggt gggagcggaa cgtcatgttc caacagcaaa cgaaacattt cgtggatata
     2041 gtcaacggca aggcacagcc ttcttgtaca cttgaagatg gggtccaagt gcagaggata
     2101 attgcttcgg tgcatgagtc tcaagagaca gggaagttga taaagttata agtgagaaag
     2161 cggcagagaa cctgtcgctt ttttgttgga ttcgctttca gggatgggaa agtttggaag
     2221 gcgtggcagg ctcgaggtcc agaaagaggc ggtgggtttc caaaattcac ccactgaatt
     2281 ttggagtata attggcgctt gtgcaaactg gtatgggcga tggatttcca tgcatcccca
     2341 tactttatgc ccacgggcat tatctaaaac aaatggagta acaatcacaa tggctaaagc
     2401 aaatcttatt accccttatg gtggaaaatt agtggacttg gtcgtgaagg gtgccgaacg
     2461 cgatgcatta attgcccgtg ccgggcatct gccttccatc aagatcacta tgcgtaactt
     2521 gtgcgacctt gaattgatcg ctacgggtgg tttttcgcct ttgacgactt tcatgggcaa
     2581 agcggactac gaacgtgtgc tcaaagaaat gcgccttgca gatggaacgc tctttccgct
     2641 gccgattaca ttgactgctg acccgaaaga attgccgacc gtgggtgaag aactcgcgct
     2701 tcgcaatgca aactttgact tgatcgctgt gatgcgcctt gatgaggtct atcactggga
     2761 tgcgaatacc gaagcggctt tggcatacgg ctcgaccgat accaagcacc cgatggtttc
     2821 tgaaatggga cgttggggaa aagtgtgtat ctctggtcct ttgcaggtga tcaaccttcc
     2881 caagtattat gactttgtaa
//
LOCUS       DAOQNJ010000345         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25141_length_2896_cov_11.414643, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000345 DAOQNJ010000000
VERSION     DAOQNJ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25141_length_2896_cov_11.414643"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..399)
                     /locus_tag="PLG52_09780"
     CDS             complement(28..399)
                     /locus_tag="PLG52_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNB41778.1"
                     /translation="MPRPITIQNLSRTLEKPATVGYCDSFQYKLRGLMFRSHLDINDG
                     LLLVEKRNSRLDTSIHMFFVPFDLTVVWINSDLTVVDKVLAKSWKPAYFPKADAQYTL
                     EIHPDRWADYEIGDKVEFKNA"
     gene            complement(452..838)
                     /locus_tag="PLG52_09785"
     CDS             complement(452..838)
                     /locus_tag="PLG52_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB41779.1"
                     /translation="MDETPTKTILCVEDEPEMIDLIRLILSRKGYSVKGASGGVEGIR
                     LIKELHPDLVLLDLMMPDMDGWEVYQQMKAEASLREIPVIVVTAKAQNIDKVLGLHIA
                     KVDDYIAKPFSPQELVDSVDKVFSHK"
     gene            890..1654
                     /locus_tag="PLG52_09790"
     CDS             890..1654
                     /locus_tag="PLG52_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNB41780.1"
                     /translation="MNIITWNVNGIRAALGKNALDWAFEKKPDALCLQEVKARPEQLN
                     EEQTAQLKLPYIWNPAERPGYSGVATFYKTQPDETKLGMDAPEFDVEGRIIQTRWGNV
                     RLFNIYFPNGQRGHDRVDYKLRFYARLLEICNTLHSKGELVVITGDFNTSHMPIDLKN
                     PKANEKTSGFMPEEREWVQKYLDNGFADAYRRLYPDRVQYTWWTYRLDARARGIGWRL
                     DYFLVSEALMPRVKDVIIHEQVMGSDHCPVELVLNV"
     gene            1854..>2896
                     /locus_tag="PLG52_09795"
     CDS             1854..>2896
                     /locus_tag="PLG52_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB41781.1"
                     /translation="MNSRSQSFFVYFLLLVAIGAMIFVGLRDNSSAAEPLTISEVARM
                     IQGGQVARIIIESDDTIRVVKVNGSEETALESRKESNTTLIEQLRDYGVTPEQLAEMN
                     IEVSAPSPWAGVLSGALYLLPVLFMGGLLWFIFRQAQGSNNAAMSFGKSRARMFSGEH
                     PTVTFADVAGADEAKQELAEIVEFLKEPQKFIQLGARIPKGVLLVGPPGTGKTLLSKA
                     VSGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRHSPCIIFVDEIDAVGRQRGA
                     GLGGSHDEREQTLNQMLVEMDGFDTDTNIIIIAATNRPDILDPALLRPGRFDRRVTLD
                     RPDVKGREAILKV"
BASE COUNT          733 a          677 c          724 g          762 t
ORIGIN      
        1 aggagcagaa gagctatgaa aaaacgttta cgcatttttg aattcaactt tatcgccaat
       61 ctcataatcc gcccaacggt ctgggtgtat ttccaacgtg tattgtgcat ctgccttagg
      121 gaagtaggcg ggcttccatg attttgcaag aaccttatcc accaccgtca ggtcggagtt
      181 gatccagacg acggtaaggt caaatggcac aaagaacata tgaatggaag tgtcgagccg
      241 cgagtttcgt ttttcaacga ggagcaagcc atcgttaatg tccagatgcg agcggaacat
      301 caatccgcgc agtttgtact gaaacgagtc acaatagccc acagtggcgg gcttttccaa
      361 ggttcgacta aggttttgta tggtgatggg gcgaggcatg gggttttatg aaaaatgggc
      421 ggcgtattcg ccgcccgttt tggaatgaaa attatttatg gctaaaaact ttatcgacgc
      481 tatccactaa ctcttgtgga ctaaacggct tggcaatgta gtcatccact ttggcgatgt
      541 gtaaaccaag aactttatcg atattttgtg ctttggcagt gacaacaata acggggattt
      601 cgcgcagaga agcttcggct ttcatttgtt ggtatacttc ccaaccgtcc atatcgggca
      661 tcataagatc aagcaaaaca aggtctggat gaagttcttt aataaggcga attccttcca
      721 caccgccaga cgcgcctttt acgctatacc cctttcgaga aagaatcaac cgaatcagat
      781 cgatcatttc gggttcatct tcaacgcata ggatggtttt ggtaggtgtt tcatccataa
      841 tttttgcctt ctaaaataag tttaccataa agagaccaac caacttcgta tgaatatcat
      901 tacctggaat gtgaacggaa ttcgcgctgc acttggcaag aatgctcttg attgggcgtt
      961 cgaaaaaaag ccagatgcgt tgtgtttgca ggaagttaaa gcacgccctg aacaattgaa
     1021 tgaagaacag actgctcaat tgaagctgcc ttatatatgg aatcctgccg aacgcccagg
     1081 gtatagtggg gtagcgactt tttataagac acagccagat gaaaccaaac ttggaatgga
     1141 cgcgcctgag ttcgatgtag aaggacgcat catccaaacc cgctggggga acgtacgtct
     1201 cttcaatatt tatttcccaa atggacaacg cggacatgac cgcgtggact ataaattacg
     1261 cttttatgcc cgtttgcttg aaatatgtaa cacgcttcat tctaagggag agctggtcgt
     1321 cattacaggc gacttcaaca cctctcacat gcccatcgac ttgaagaatc ccaaagccaa
     1381 cgagaagacc tctggcttta tgccagagga acgcgagtgg gttcaaaaat atttagacaa
     1441 cggctttgca gatgcctatc gccgcctcta cccagaccgg gtccaataca cctggtggac
     1501 ctatcgcctc gatgctcgtg cccgcggaat tggctggcgc ttggattatt tcctcgtctc
     1561 agaggcgctc atgccccgcg taaaagatgt catcattcat gagcaggtga tgggttctga
     1621 ccattgtccg gtagagctgg ttttaaatgt gtaaactttg tggatggctg attaatttct
     1681 aataagttcc gttgaaatta tcccctaggg tgacaaaaga ttgattggtt ttgcaagcag
     1741 gttccggtta gactttgaac cgtgttagaa aatcgtaata ataaaagccc agacggtatc
     1801 tctactggta tggcttggta taatgaccgt gtacgtcaaa tttggaggat tccgtgaatt
     1861 cccgtagtca atcgtttttt gtgtactttt tattgctcgt agccatcgga gcgatgattt
     1921 ttgttggctt gcgagacaat tccagcgctg ccgagccgct caccatcagt gaggtggcac
     1981 ggatgattca gggcgggcaa gtagcacgca tcatcattga gagcgacgat accattcgtg
     2041 tggtaaaggt caatgggtct gaagaaacag cccttgaatc ccgcaaagag tcaaatacga
     2101 cccttatcga acaactccgt gattatggtg taacccccga gcaacttgct gaaatgaata
     2161 ttgaagtcag cgcaccatcg ccatgggcgg gtgtgttgag cggcgctttg tatctgttac
     2221 cggttctgtt tatgggtggg ttgctttggt tcattttccg ccaggcacaa ggcagcaaca
     2281 atgcagcgat gtcctttggt aaatctcgtg cgcgcatgtt cagtggggaa catcccaccg
     2341 tcacctttgc cgacgttgcc ggcgccgacg aagccaaaca ggaattggca gagatcgtcg
     2401 agttcctgaa agagccgcag aagtttatcc aacttggtgc tcgtattccg aagggtgttt
     2461 tgttggtggg ccctcccgga accggtaaaa cccttctttc taaagctgtt tctggtgaag
     2521 caggcgtgcc cttcttctct atctctggtt ctgaattcgt agaaatgttc gtaggtgtgg
     2581 gcgccagccg tgtgcgtgac ctgttcgacc aagccaagcg ccattcgcct tgtattatct
     2641 ttgtagatga aattgatgcg gtgggtcgcc aacgtggagc aggactcggc ggttcacacg
     2701 atgaacgcga gcagaccctc aaccagatgc tcgtcgaaat ggacggtttc gataccgaca
     2761 ccaacatcat catcattgcg gcaaccaacc gccccgatat tctggacccg gctcttctcc
     2821 gccccggtcg ctttgaccgc cgcgtaacgc ttgatcgccc cgatgtaaag ggacgtgaag
     2881 ccatcctcaa ggtgca
//
LOCUS       DAOQNJ010000346         2895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25155_length_2895_cov_8.932394, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000346 DAOQNJ010000000
VERSION     DAOQNJ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25155_length_2895_cov_8.932394"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /gene="glpX"
                     /locus_tag="PLG52_09800"
     CDS             <1..717
                     /gene="glpX"
                     /locus_tag="PLG52_09800"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNB41782.1"
                     /translation="PIDGTRPLAFGRSNSLATVALAPRGTMFDPGPFLYMNKIAVGPE
                     AKGKIDIEKSITENLHAIAKAKGKAVEDLTTIILDRPRHDDMIAEIRKAGARIREIPD
                     GDVAAALMTAWPDAGVDVLLGIGGTPEGVLAACALRAMGGEIQGKLFARDEDELRRGR
                     EQGYNFDMVLTMNDLVASEDVFFAATGITDGELLKGVRYYGDRITTDTLVVRGLTGTV
                     RQITATHKTDKLDNLSSVHY"
     gene            766..841
                     /locus_tag="PLG52_09805"
     tRNA            766..841
                     /locus_tag="PLG52_09805"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:799..801,aa:Asn,seq:gtt)
     gene            complement(891..1670)
                     /locus_tag="PLG52_09810"
     CDS             complement(891..1670)
                     /locus_tag="PLG52_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41783.1"
                     /translation="MKRALIFTCILFVLSACALASNPTAEPASATEELILANSATATA
                     LPPTETFTPSPIPPTETPSPTATATPFPIVESLKAIVTADKLICRYGPGANYLYLIAF
                     NINTPLRLIGRAPGNNWVLVQNEPQKCWVNSEFVALKEEGDFRSLPSMYPDGYKIPVS
                     PYYGPTKVLTAERNGNDITISWIEIIVSPGKYEDENMFPYIVEVWTCKDGQIVFDTIG
                     SRVPFITFTDEPGCSEPSHGRVYIQEKHGYAGPAEIPWPEN"
     gene            1781..>2895
                     /locus_tag="PLG52_09815"
     CDS             1781..>2895
                     /locus_tag="PLG52_09815"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HNB41784.1"
                     /translation="MNAFIPQRDLPKQLLAVLSGGFVLFIGIVLVWTVGYQLAYAGRI
                     FPGVTVAGVDLSGLSPSEAAVKLSQTLSYPNTGKVLFRDGERVWVASPAELGMVFDPT
                     ASANAAYEHGRRGGLFASLSGQISARGLGMDIPPVVLFDQRVAYAYLQNIASQIDQPV
                     AEAVLRVDGTNVIAEPGRVGRALNLDATLIYLGSQLQSFRDGEVSLVIQETSPLLLDV
                     TPQADVARAMLSQSFTMTLPNVGSGDPGPWTFDVPVLANMLVFNKVDAGGVPQLQVGL
                     DTNALRKSLSELKVVVDREPQDARFIFNESKGQLEAISASTIGRAVDVEASILAINGA
                     LQRGEHSVNLVVTEKQPAVTDQATGADLGIVQLVATQT"
BASE COUNT          682 a          653 c          781 g          779 t
ORIGIN      
        1 cccatcgatg ggactcgccc tttggctttt ggtcgctcca attcgcttgc aaccgtggca
       61 ttagcaccgc gtggaaccat gtttgacccc ggtccgtttc tttacatgaa taagattgca
      121 gtgggacctg aagcaaaagg caagattgat attgaaaaat cgatcactga aaacctgcat
      181 gccatcgcaa aagcaaaagg caaagcggtt gaagacctga ccaccatcat cctcgaccgt
      241 ccgcgccacg atgacatgat tgcagagatc cgcaaagcag gtgctcgtat tcgcgaaatt
      301 cccgacggcg atgtggctgc ggctctaatg acggcttggc ccgatgctgg tgtggatgtt
      361 cttcttggta ttggagggac gccagaaggc gtgctagcgg cttgtgcctt gcgtgccatg
      421 ggtggagaaa ttcagggcaa attatttgcc cgtgatgaag atgaacttcg ccgtgggcgt
      481 gaacaaggtt ataacttcga catggtacta accatgaatg accttgttgc ttctgaagat
      541 gttttctttg ctgccacagg tatcacagat ggggaactgc tcaaaggtgt gcgatattac
      601 ggcgaccgta tcaccaccga cacattggta gttcgcggtc tgaccggcac cgtccgccag
      661 atcaccgcca cgcacaagac cgataaattg gataatttga gctcggttca ttactagaaa
      721 attgcttgac attcctgggg catgtgcata taataccgct cgcgttcctg gctagctcaa
      781 tcggcggagc gagcggctgt taaccgctag gttataggtt caaatcctat gccaggagcc
      841 atacaaaaag tccttgagtt ctcaaggact tttttatttt taaattttaa ctaattttcc
      901 ggccaaggaa tttctgctgg acctgcatac ccgtgctttt cttggatgta aacccgtcca
      961 tgtgagggtt cggaacagcc gggttcgtcg gtgaaggtga tgaaggggac gcgagaaccg
     1021 atggtatcaa agacgatctg accatctttg catgtccata cttcgacgat ataggggaac
     1081 atgttctcgt cttcgtattt gccggggctg acaatgatct ctatccatga gatagtgatg
     1141 tcgttgccat ttcgttcagc ggtgagcacc ttggttggac cataatatgg tgaaacggga
     1201 attttatagc catctgggta catcgatgga agggagcgaa agtcgccttc ttcttttagg
     1261 gctacaaact ctgagttgac ccaacatttt tgcggttcat tttgaacgag cacccaatta
     1321 tttcccggtg cacgcccgat cagacgcaaa ggtgtgttaa tgttgaatgc aataagataa
     1381 agataatttg cgcctggtcc atagcggcaa atgagcttat cagcagtgac aatggctttt
     1441 aagctttcaa cgatagggaa tggcgtggca gtagccgtag ggctgggcgt ttcagttgga
     1501 gggatggggc tgggtgtgaa ggtttctgtg ggtggtaggg ctgtggcggt agcgctgtta
     1561 gcgagaatca attcttcagt ggcagaggca ggctccgctg tggggtttga ggcgagcgcg
     1621 caagcgctta gaacaaagag aatacaagta aaaatcagag cgcgtttcat ggtttttcca
     1681 tccgatccaa ataaattttc tcaattgtac cgaaaagttt tcattcaaaa cttagatact
     1741 attcataatc gcccgatttt tttcgttata atctttgacc atgaacgcat tcattcccca
     1801 acgtgatttg cccaagcagt tgcttgccgt gttgagcggt gggttcgtgc tttttattgg
     1861 tatcgttctt gtgtggacgg ttggatatca acttgcctac gccggacgca ttttccctgg
     1921 agtgacggtt gctggtgtgg acctttcggg tctttctccc agtgaggctg ctgtgaagtt
     1981 gagccagaca ctttcttacc cgaatacggg aaaagttctt ttcagagatg gtgagcgcgt
     2041 gtgggtggca tcgcctgcag agttaggcat ggtgttcgac ccaactgcca gtgccaatgc
     2101 tgcatatgaa catggacgtc ggggcgggtt gtttgcttca ctatcaggac aaatcagcgc
     2161 ccgcggactt ggtatggata ttccaccggt tgtgctcttt gaccaacggg ttgcatatgc
     2221 atacttgcaa aacatcgcct ctcaaatcga ccagcctgta gctgaagctg ttctgcgagt
     2281 agatggaact aatgtgatcg cagagccggg gcgtgtggga cgcgcactca acctcgatgc
     2341 aaccttgatc tatcttggtt cgcagttgca aagttttcga gatggagaag tttctctcgt
     2401 gattcaagaa acatccccac ttttgttgga tgttacgcct caagcggatg tggcgcgtgc
     2461 catgcttagc cagtcattta cgatgacatt accaaatgtc ggttctggtg accccggtcc
     2521 atggactttt gacgtgcctg tgttggcaaa catgttggtc ttcaacaaag tggatgcagg
     2581 cggtgtgccg caattacaag ttggtttgga tacgaacgct ttacgcaaat cgttgagtga
     2641 actgaaggtc gttgtagacc gtgagcctca agatgcacgc tttatcttca acgagtcaaa
     2701 gggacaactt gaagcgatct ctgcttccac gattggacgt gctgtggacg tggaggctag
     2761 tattctggca attaacggtg cgttacaacg cggagaacac agcgttaact tggttgttac
     2821 agaaaagcag cctgcagtga cagatcaagc cactggcgca gaccttggca ttgttcagct
     2881 cgttgccacc caaac
//
LOCUS       DAOQNJ010000347         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25363_length_2880_cov_11.823717, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000347 DAOQNJ010000000
VERSION     DAOQNJ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25363_length_2880_cov_11.823717"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLG52_09820"
     CDS             complement(<1..303)
                     /locus_tag="PLG52_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41785.1"
                     /translation="MNFDYGNILTRALKLTWKHKSFWLLLMIPVFISFMILAAIILPV
                     IFLTENEESMTLIMVLWAGIVIFGFLASFIIGAVSMNAVTLGILRAERGEGSTALMD"
     gene            complement(336..1871)
                     /gene="guaA"
                     /locus_tag="PLG52_09825"
     CDS             complement(336..1871)
                     /gene="guaA"
                     /locus_tag="PLG52_09825"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNB41786.1"
                     /translation="MNSIAILDFGSQYAQIIARRVRESQVYCELFPWDAPQEKILSIK
                     PKGFILSGGPKSVYEENAPFIQKFILESGLPILGICYGMQALTHALGGKVDPSAQREY
                     GHAEITPLVPGTMLDVLSKVWMSHGDKVTQLPEGFMALAKSGNSPFAAMGDMKRKYFG
                     VQFHPEVHHTPNGTELIKHFAIDICGVTPDWTPANIIEESVKRIREQVGNERVLAAVS
                     GGVDSSVAAALVHKAIGDQLVCVFVDTGLLRANEGAQVASAFREGLGAELITVDAADD
                     FLGALKGQTDPEMKRKIVGEKFIRTFEREAKNVGQPRFLVQGTIYPDVVESSGPDRSK
                     AAKIKSHHNVGGLPEDMQFELVEPLRYLFKDEVRLVGEALGLNSSLVWRQPFPGPGLT
                     VRCLGECTPERVARLRAADAILLEELSKAGFLGKGAQTSQAFVVLLPVRSVGVMGDMR
                     TYQEAAAIRAVATDDFMTADWARLPFELLAKVSSRIVNEVEGINRVVYDITSKPPATI
                     EWE"
     gene            complement(1905..2252)
                     /locus_tag="PLG52_09830"
     CDS             complement(1905..2252)
                     /locus_tag="PLG52_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNB41787.1"
                     /translation="MIRKILHLRFIVFLIGWIFAHMSFAIPVKRLRETSNLIAFQHPS
                     PSYKVHLLITPKRQVQSLAELDPQDTAFFTDLYSTVQSLVKELDLKAYRLIVNGGEYQ
                     DFPHLHFHLISEM"
     gene            complement(2286..>2880)
                     /gene="xpt"
                     /locus_tag="PLG52_09835"
     CDS             complement(2286..>2880)
                     /gene="xpt"
                     /locus_tag="PLG52_09835"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNB41788.1"
                     /translation="SVQTIGGFMNALQERILKEGKVLGGGILKVDSFVNHQVDPVLMD
                     ECGRAFAKLFAKVGATKILTAEISGIAPALTTGIHMGLPVVYARKTKPVTMPDQVYLT
                     LAPSHTKGRMVELIVSPEYLANNEKVLIIDDFLASGQTILGLVRLAEASGSKVVGIGA
                     LVEKSFEGGRDALASVGVQIESIACIKSLDDGKIEFV"
BASE COUNT          756 a          712 c          715 g          697 t
ORIGIN      
        1 atccataagc gcagtagaac cttcaccgcg ctcggctcgc aaaatgccaa gtgtgactgc
       61 attcatacta actgcgccga taataaaact agcaagaaac ccaaaaatga caatcccagc
      121 ccaaagcacc atgatcaggg tcatagattc ttcgttttca gttaaaaaga tgaccggcaa
      181 aatgattgct gcaagaatca tgaaagaaat gaacacggga atcatcaaga gaagccaaaa
      241 cgatttatgc ttccaggtga gctttaatgc gcgggttaag atattgccat agtcgaagtt
      301 catttccatc tcccaattgt caatcgaaaa ttgatttact cccactcaat ggttgcaggc
      361 ggtttgctcg ttatatcata gactacacga ttaatgcctt ctacttcatt gacaatacgg
      421 ctcgagacct ttgctaataa ttcaaatggc aggcgagccc aatcggcggt catgaagtca
      481 tccgttgcta cagcgcggat cgctgctgct tcttgatagg ttcgcatgtc acccatcacg
      541 cccaccgaac gaaccgggag taacacaaca aatgcctgtg acgtttgagc gcccttgccc
      601 agaaaacctg ctttggataa ttcctccaga aggatcgcat ctgccgcccg cagacgtgcc
      661 acacgctcgg gcgtacactc cccgaggcag cgcacggtca gaccgggacc ggggaacggt
      721 tgacgccaca ccaaactgga attcaagccg agggcttcac ctaccaaacg gacttcgtcc
      781 ttgaataaat agcgcaacgg ttccacgagt tcaaattgca tatcttcagg gagtccgccc
      841 acattgtgat gagacttgat cttggctgct ttgctgcggt cgggaccgga tgactcaact
      901 acatcggggt agatcgtgcc ttgcactaag aatctaggtt gacctacatt cttggcttcg
      961 cgttcaaagg tgcggataaa tttctcgccg acaattttgc gcttcatctc tgggtcagtt
     1021 tgccccttca atgcgccgag aaaatcgtca gcggcgtcga cggtgatgag ttcagcaccc
     1081 aagccttctc gaaatgccga agcgacttgt gcgccttcat tggcgcgcaa gagtcccgta
     1141 tccacaaaga cacagaccag ttgatcgcca atcgctttat gaactaatgc cgccgcaaca
     1201 gaggaatcca cgccaccgga aacggctgcg agcacacgtt cgttgcccac ttgttcacga
     1261 atccgtttta cgctttcttc gatgatgttg gcgggagtcc aatcgggcgt tacgccacaa
     1321 atgtcgatgg cgaagtgctt gatgagttcg gtgccgttcg gggtgtgatg cacttcgggg
     1381 tgaaattgca caccaaaata tttgcgtttc atatcgccca tcgccgcgaa gggactgttt
     1441 ccacttttgg cgagtgccat gaaaccttcg gggagttgtg tcactttatc gccgtgtgac
     1501 atccaaactt tggagagcac atccaacatg gtgccgggaa ccaatggcgt gatttcggca
     1561 tgaccatact cgcgctgggc agatgggtcc actttgccgc cgagggcgtg ggtgagggct
     1621 tgcatgccat aacaaatgcc aaggatggga agcccgcttt cgaggatgaa tttttgaatg
     1681 aagggtgcat tctcttcata aacagacttg ggtccgccag ataaaataaa tcccttcggt
     1741 tttatcgaaa ggattttttc ttgcggcgca tcccatggaa agagttcaca atagacttgt
     1801 gactcgcgca cacgtcgggc gataatttgg gcgtactgag aaccgaaatc taaaatggcg
     1861 attgaattca tgtgttttat taatcccgta ggggggactc acccctacat ttctgaaata
     1921 agatgaaaat gtaaatgtgg aaaatcttga tactctccgc cattgacgat tagccgatac
     1981 gctttgaggt caagttcttt tacgaggctt tgcacagtgg agtataagtc tgtaaagaaa
     2041 gcggtgtctt gtgggtcaag ctctgccaac gattgaacct gtcgcttcgg ggtgataagc
     2101 aaatgaacct tataactggg agaaggatgc tggaacgcaa tcaggttcga ggtctcacgg
     2161 aggcgtttga ccgggatggc gaaactcatg tgagcgaaga tccaaccaat gaggaaaaca
     2221 atgaagcgca ggtgtaggat tttacgaatc acggttcact ctttgcaaag agtgaaccgt
     2281 gtgtattaga caaactcaat cttgccatca tccagtgact tgatacaagc gatggattca
     2341 atttgcacac caacagaagc gagagcatca cgcccgcctt cgaaactctt ttcgaccaac
     2401 gcgccaatgc caacgacttt ggaaccagag gcttctgcca aacgtacaag cccaagaatg
     2461 gtctgtccgc tggcgaggaa gtcatcgatg atgagaactt tttcgttgtt ggcaagatat
     2521 tccggtgaga cgatcaactc caccatgcga cctttggtgt gagaaggggc gagggtgaga
     2581 tacacctgat cgggcatggt aacgggtttg gtcttgcgag catacacaac gggcaaaccc
     2641 atgtgaatgc cagtggtgag cgccggagca atgcccgaga tttctgctgt gagaattttg
     2701 gttgcaccca cttttgcaaa gagtttggca aacgcacgcc cgcactcatc catgagcaca
     2761 gggtctactt ggtgattaac aaaactatct actttaagaa ttccaccgcc gagcactttc
     2821 ccctctttta aaattcgttc ttgcaaagca ttcatgaagc ctccgattgt ctggacagat
//
LOCUS       DAOQNJ010000348         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25521_length_2869_cov_11.398010, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000348 DAOQNJ010000000
VERSION     DAOQNJ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25521_length_2869_cov_11.398010"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="panB"
                     /locus_tag="PLG52_09840"
     CDS             <1..774
                     /gene="panB"
                     /locus_tag="PLG52_09840"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNB41789.1"
                     /translation="AYDYPTAVAIDKAGIDSILVGDSLAMVVLGYENTLPVTMDEMLH
                     HARAVSRGAKAPLLVGDMPFMSYQVSVEDAVRNAGRFLQQGGMDAVKLEGGRERADAV
                     RAIVNAGIPVMGHLGLTPQSVNQLGGFRAQGKTASAAKRLLEDAQILEEAGAFSLVLE
                     SVPARLAEYISKHISIPTIGIGAGTGCDGQVLVTHDLLGLFERFTPKFVRQYAKLHET
                     MNKAFTEYLNDVNTKQFPAAEHTVEMTDEEWAAFQKEVK"
     gene            771..1667
                     /locus_tag="PLG52_09845"
     CDS             771..1667
                     /locus_tag="PLG52_09845"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNB41790.1"
                     /translation="MSENILLIGTGALSTLFAARLSEAGHHVSMLGTWKDGIQALNQN
                     GARVSESNGKEHAFQVHATDEPSEVRNVKHAIVLVKSWQTKRVAEKLKGILSPDGIVL
                     TLQNGLGNRETLARDLGAERVALGVTTTGATLLGPGHVKVGGEGILSLEQNQALGPLE
                     AALRSSNFNLQIVQNAQSLVWGKLIINAAINPLTALLQVPNGELLNHPWARKAMNALA
                     REAAEVAQAEQVNLPFNNPIEAVEEVARKTAQNLSSMFQDVRRGAPTEIDAICGAVTK
                     RGEMHGISTPYNRSCWQLVKAM"
     gene            1815..2717
                     /locus_tag="PLG52_09850"
     CDS             1815..2717
                     /locus_tag="PLG52_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNB41791.1"
                     /translation="MRKLFLLLLMGAFLSFPISASAQSDVSLSFATVQLWPEYDDPSM
                     LVIVDFQVAATTSLPATLTFRIPAEANLIAVASDTGNGQFLDHPYTGPNPEGEYQTFS
                     MVLEQNTPYRFEYYQPLSYNGDTRIFSYLWDSEYAVENFQYLFLEPLDVTDIALDPKY
                     TAKETFNGLNYYDGAPVQLAAGEQYSMKVSYTKTTDTLVSQAQSVQIAEPVNEDTPGR
                     VSLANSLPYIIGGLGIIMIAGGLMYYFQWGRHSAGGGKHRRRSRTNTESDATSIYCPQ
                     CGARAKPSDRFCRTCGARLRHDEA"
BASE COUNT          746 a          690 c          720 g          713 t
ORIGIN      
        1 gcatatgact atcccaccgc agtggcgata gacaaagccg ggattgattc cattctggtg
       61 ggcgattcac ttgccatggt ggtgcttggt tatgaaaata ccttgcccgt caccatggac
      121 gaaatgctgc atcatgcccg tgctgtatcc cgcggagcaa aagccccatt actcgttggc
      181 gatatgccat ttatgtcgta tcaagtctca gtggaagatg ctgtgcgcaa cgctgggcgt
      241 ttcttacagc aaggtggaat ggacgcggtc aagcttgaag gcgggcgtga acgagctgat
      301 gcagtgcgcg ccattgtcaa tgcaggcatt cctgtgatgg gtcacctcgg gcttacgccg
      361 caatcggtga atcaacttgg tgggttccgt gcacagggca aaacagcgtc tgcggctaaa
      421 cgtctacttg aagatgcgca gattcttgaa gaagctgggg cgttcagcct cgtgctcgag
      481 tctgtgcccg caagattggc ggaatatatt tcaaaacaca tttccattcc aaccattggt
      541 attggcgcag ggacaggctg tgatggacaa gtgcttgtca cccacgactt actgggtcta
      601 tttgaacgct ttacaccgaa atttgttcgc caatacgcaa agttgcacga aaccatgaac
      661 aaggctttca ccgaatatct caacgatgtg aacaccaaac aatttccggc tgccgaacat
      721 accgttgaaa tgacagatga agaatgggct gcttttcaaa aggaagtgaa atgagcgaga
      781 atattttact tattggtaca ggcgcgctct ctacattgtt tgcagcccgt ttgagcgaag
      841 ctgggcatca tgtatccatg ttgggcacat ggaaagatgg cattcaagct cttaatcaaa
      901 acggcgctcg tgtatcagaa tcaaacggaa aagaacacgc cttccaagtc catgctaccg
      961 atgaacctag tgaagttcga aatgttaaac acgccattgt cttggtcaaa tcttggcaga
     1021 caaaacgtgt ggcagaaaaa cttaagggga ttctctcacc cgatggaatt gtgttgaccc
     1081 tgcaaaacgg gcttggaaat cgcgaaacgc tcgcacggga ccttggggct gagcgcgttg
     1141 ccctaggcgt taccaccact ggagccaccc tgcttggacc tgggcacgta aaagttggcg
     1201 gcgagggcat cctctcactg gaacagaatc aggctctcgg tccgcttgag gcggctcttc
     1261 ggtcatccaa ctttaatttg cagatcgttc aaaatgcgca atccttggtg tggggcaaac
     1321 tcatcatcaa tgcagctatt aacccgttga ctgcgttgtt gcaagtgcca aacggtgagt
     1381 tgttgaatca tccttgggca cgcaaggcga tgaatgctct ggcgcgtgaa gccgcggaag
     1441 tggcacaggc agagcaggtg aatttgccat tcaacaaccc gatcgaagcg gtggaagaag
     1501 tggctcgcaa aacggcgcaa aatctttcat caatgtttca agatgtacgg cgcggtgctc
     1561 cgacagagat cgatgccatt tgtggtgcgg tgaccaaacg cggagaaatg cacggaatta
     1621 gcacgccata caatcgttct tgttggcaat tggtaaaagc aatgtaatta agtacgggcg
     1681 gcgcatgcgc cgcccgtacc gatataaaaa gggtgacatt tatctatatt ctgcatgatt
     1741 tcgtttctgc cctcaaaaaa tgcgacatgg taagatacat atagaagtaa aacatttatc
     1801 gttaggaggc gttcatgcgc aaactattct tactccttct tatgggagcc ttcctctcgt
     1861 tcccgatttc cgcatctgca caaagcgatg tgtcgctttc atttgccact gtgcaattat
     1921 ggcccgaata tgatgacccc agcatgctgg tgatcgtaga ttttcaagta gcggcaacta
     1981 caagtttgcc tgctacactc accttccgta ttcctgcaga agccaacctc atcgctgtcg
     2041 cttcagatac aggcaacgga caattcctcg atcacccata cacaggaccc aaccccgaag
     2101 gtgaatatca aacctttagt atggttcttg agcaaaatac cccctatcgt tttgaatact
     2161 atcaaccgct ttcatacaac ggggatactc gcattttctc ttatctttgg gatagcgaat
     2221 atgcggttga gaattttcaa taccttttcc ttgagccttt agatgtcacc gatattgcgc
     2281 tcgacccgaa gtacactgcc aaagagacct ttaacggatt gaactattac gatggtgcac
     2341 ctgttcaact tgcggcgggt gaacaatatt cgatgaaagt aagttacacc aaaactacag
     2401 atactttagt ctcgcaggca cagtcggtgc aaatcgcaga accggtgaac gaagatacgc
     2461 caggtcgcgt ttcacttgcc aatagtttgc cttacatcat tggcggactt ggcatcatta
     2521 tgatcgcagg cggcttgatg tattacttcc aatggggacg ccactctgca ggcggtggta
     2581 aacatcggcg ccgttcacga acgaatacag aaagcgatgc caccagcatc tactgtccac
     2641 aatgtggggc acgtgccaag cctagtgaca ggttctgtag aacctgtgga gcaaggctaa
     2701 ggcacgacga agcataacga ggagagaccc taaaggcgaa caaagcgcct ttagggtctt
     2761 tttatgtaag catggaaaat ctgtctgtta cagtcatcta tttcttttat gggcttgcct
     2821 ttttcagcat gggcttgttg gttgccatgg aaggagggcg ctctacaga
//
LOCUS       DAOQNJ010000349         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25535_length_2868_cov_8.337718, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000349 DAOQNJ010000000
VERSION     DAOQNJ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25535_length_2868_cov_8.337718"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="PLG52_09855"
     CDS             <1..1052
                     /locus_tag="PLG52_09855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNB41792.1"
                     /translation="YTENYCMQNIEFGLCTFGDIPQDDSGKLVSYARAIRQVVDEAVL
                     ADTIGVDVISLGEHHRLEFAISSPETVLAGIATKTNRIRLASGVTVLSSDDPVRVFER
                     FATLDALSNGRAEVILGRGSFTESFPLFGYDLRDYEILFEEKIQLFAELLKEKPVTWK
                     GTKRAPLENADVFPKTESGRLSTWVGVGGSPESVIRTARYGFSLVLAIIGGSPQRFAP
                     YVELYKRAAAELGTTAQAVGVHSPGFVADTDEEAWEMYLPNARVVRERIGRERGWPPS
                     SLQEYQRERDTGSLYVGSPETVAKKIARTIKTLGINRFDLTYTMGAQSIRSRLRAVEL
                     YGTKVIPMVREILGS"
     gene            1057..1761
                     /locus_tag="PLG52_09860"
     CDS             1057..1761
                     /locus_tag="PLG52_09860"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNB41793.1"
                     /translation="MSATDIKTIGILGAGKVGTVLARLALSAGYRVLIAGSGDPGKIA
                     LTIEVLTPGAVAVTAQEAASQADVVILALPLGKYKNIPAEALQGKLVIDAMNYWWEVD
                     GIRDDLNDPLTSSSEIVQAYLRGARVIKAFNHMGYHDLEEGALPAGAPERRAIAIAGD
                     DPIDIQTVAGLVDTFGFDPVIVGPLADGMRLGPGTEAFGANVGADELRAMLERFPESE
                     RGKVILQARAAVDDRQ"
     gene            complement(1802..2374)
                     /locus_tag="PLG52_09865"
     CDS             complement(1802..2374)
                     /locus_tag="PLG52_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB41794.1"
                     /translation="MLPNPREEEKLDPRVKRTRGLILQSFESLLAEKGFETISVQDVT
                     DKAQVNRATFYAHFPDKYALLDYSIQKAFMSEIEKRTLNVCSYSPDNLRNLILAVVEF
                     LSRVHTGCAQPHQQFESLIEGTIKKQIFDLLSYWLRKSNVPTEIPATVASWAIYGLAS
                     HYSHMKKRPALEKFVDEAYPLVAVNLEQFA"
     gene            2510..>2868
                     /locus_tag="PLG52_09870"
     CDS             2510..>2868
                     /locus_tag="PLG52_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNB41795.1"
                     /translation="MNIALWVIQVLLAGMYGMAGSMKTFTPDKVRTNPQMTWAHDKQD
                     GYIRFVGISELLGAVGLIIPALTGILPWLTPLAAIGLALIQVLAIFTIHLPKKEFQVI
                     PVNVVLLVLAIVAAYGR"
BASE COUNT          657 a          663 c          807 g          741 t
ORIGIN      
        1 tttatacgga gaattactgt atgcaaaata ttgaatttgg gctatgtacg ttcggcgata
       61 taccccagga cgactctggc aaacttgtat catatgccag ggcgatccgt caggttgttg
      121 atgaggctgt gctggcggat acgattggcg ttgatgtcat ttcccttgga gaacaccaca
      181 gactggagtt tgcaatctca agcccagaga ctgtacttgc aggcattgca acaaagacca
      241 atcggattcg tcttgcttcc ggcgtgaccg tgttgagttc cgatgaccca gtgcgtgtgt
      301 ttgaacgttt tgccacgttg gatgcgctct cgaatgggcg ggctgaagtt attctggggc
      361 ggggttcctt tacggagtcg ttccctctct tcggctatga cttgcgagac tacgaaatat
      421 tattcgaaga aaagatccaa ctatttgctg agttactgaa ggaaaagcct gtcacttgga
      481 aaggcaccaa aagagcgccg cttgaaaacg cagatgtctt cccaaaaacc gagtcagggc
      541 ggctctcgac atgggtgggt gttggcggtt cgcctgagtc tgtgatccgc actgcacgct
      601 atggattttc acttgtgctt gcgattattg gcggctctcc gcaacgcttc gctccttatg
      661 ttgaactcta taaacgtgct gcggcggagt tgggtacaac cgcgcaggca gttggagtac
      721 actcgcccgg ctttgtcgct gacacagacg aagaggcttg ggaaatgtat ttgccgaatg
      781 cgcgggtcgt ccgcgagcgg attggcaggg aacgtggttg gccgccatcc agtttgcagg
      841 aatatcagcg cgaacgggat acagggtcgc tctatgttgg ctcgccagaa acagttgcca
      901 aaaagatcgc ccgtaccatc aaaacccttg gcatcaaccg cttcgacctt acgtacacaa
      961 tgggcgcaca atctattaga tcccgcctgc gtgcggtgga gctgtatggc acaaaagtaa
     1021 ttcccatggt gcgggaaatt ctgggaagtt aactctgtga gcgcgacgga tattaaaaca
     1081 attggaatcc taggtgctgg gaaagtggga accgtactgg cacgtcttgc cttgtcggca
     1141 ggttatcgcg tgcttattgc ggggtcaggt gatccgggta agatcgctct caccatcgaa
     1201 gtcctcacac ccggcgcagt tgctgtcact gcccaggaag ccgcttcaca agcggatgtg
     1261 gtgatccttg cgcttccgct tggcaaatat aaaaatatcc ctgctgaagc tttgcaggga
     1321 aaacttgtca ttgatgccat gaattattgg tgggaagttg acggtatccg tgacgatctg
     1381 aatgacccgc ttacttcatc aagtgagatc gtgcaggctt acctacgcgg cgcgcgcgtc
     1441 atcaaggcgt tcaatcatat gggctatcat gatcttgaag aaggcgcatt acccgctgga
     1501 gcacccgaac gcagagcaat cgccattgcc ggggatgacc cgattgatat tcaaactgtt
     1561 gcggggcttg ttgatacttt cggctttgac cctgtcattg tgggtccgct tgccgatggg
     1621 atgcggctcg gaccgggcac tgaggctttt ggcgccaacg tcggcgcgga tgagctgcgg
     1681 gcaatgcttg agcgcttccc agaatcagaa cgtgggaagg ttattttgca ggcaagggcg
     1741 gcagtggacg atagacaata gacgattgac cgcggtctat cgtctgccgt ccgcggtcgt
     1801 attaagcaaa ctgctctaaa ttaactgcta ctaacggata cgcttcgtcc acgaattttt
     1861 cgagggcggg gcgttttttc atgtggctgt agtgcgaggc gagtccgtag atcgcccagg
     1921 aggcaaccgt ggcggggatt tcggtaggga cattcgactt gcgcaaccaa taagaaagca
     1981 aatcaaatat ctgcttcttg atcgtccctt cgattaggga ctcaaattgt tggtgcggtt
     2041 gggcacagcc agtatggacg cgggaaagga attccactac tgccaggatc aggtttcgca
     2101 aattatcagg ggaatagctg cacacgttca atgtccgttt ttcaatttcg gacatgaatg
     2161 ctttttggat cgagtaatcg agcagggcat atttatcggg gaaatgagca tagaacgtgg
     2221 cgcggttgac ctgtgctttg tcggtcacat cctgcacgga gatggtttcg aatccttttt
     2281 cagcgaggag agattcaaag gattgcagaa tcaagcctcg ggtgcgtttg acgcgggggt
     2341 ccagcttttc ttcttctctt gggttaggca acatttttgc tcctttgttg cttagtcaac
     2401 aattgaaagt ctttgttggt tgactttcaa agtggggatt ggtaaattag gcaacatctg
     2461 ttgcgtaaac aacgactgta taatatcaaa caactcaagg agagtcaaga tgaacatcgc
     2521 attatgggta atacaagttt tactagctgg catgtacggg atggcgggca gcatgaaaac
     2581 cttcacgcca gataaagtgc gcacaaatcc gcagatgacc tgggcacacg acaaacagga
     2641 tggctacatc cgctttgttg gaatctctga attgcttggc gcagtgggat tgatcatccc
     2701 tgccctgact ggcattttgc cctggctgac cccgctggct gcgatcgggc tggcgcttat
     2761 tcaagtgtta gccattttca caattcacct gccgaagaaa gaatttcagg ttattcctgt
     2821 gaacgttgtt ctgttggtgc tggcgatcgt tgcagcatat ggtcgctt
//
LOCUS       DAOQNJ010000350         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25601_length_2864_cov_5.361695, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000350 DAOQNJ010000000
VERSION     DAOQNJ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25601_length_2864_cov_5.361695"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2175)
                     /locus_tag="PLG52_09875"
     CDS             complement(<1..2175)
                     /locus_tag="PLG52_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB41796.1"
                     /translation="MSDYENSIFISYAWGEETDEREAIVNRLDQSLQKRGIKIVRDKR
                     DLGYKGMIREFMRRIGEGNCVIVVISDKYLRSKNCMFELVEIAANKDFADRIFPIVLS
                     DAKIYDALDRIDYLTYWEKKKSELDQKMRSLKELANIPGISEELDDYDRFRDEIDRLS
                     TVIKNMNTLSPDILRDSDFQQLYDALNQRMGPSWTASNSSPSPAPSANHDLPLGSYLP
                     FPRNAIFTGRVADLETLVQSLLDSNDTGAVINQTITGMGGLGKTQLAVEFAYRHGYQF
                     KGVHWLDVREVQNIESQIALCGSKMNLPNFPPTQPEQVAVTIHEWKMNGPRLLILDNF
                     EEYNVANEILGVLHHSNLKVLITSRHTNWSAGLGLKRLPLGEFSSTESLEFLRKYIAP
                     ERENDVALEKLAEHLGYLPLALELAGRYLEKQPRLKIQEYLEQTKNALSHKSMQNWKV
                     EQKSLTGHDLSLAQTFAQSWIFVKDETAQKLFTTLGYCAPNTPIPQAILDAALGDPTD
                     ACDEAISELTGLGLLKEGHSIHPLLAEFARGLDEDNHLLKPFSEALANLANQTNSDED
                     QTGNYALYTPLLPHLRVVAEYAEAAALEPAGELWNSIGYHIKDLADHAGAKAAFERAL
                     KIFENVLGEDHPNVATLINNLGGVLKALGDHAGAKAAFERALKIDEATFGPDHPDVAI
                     DVNNLGMVLKDLGDHAGAKAAFERALKIDEATFGPDHPSVAIR"
     gene            complement(2249..>2864)
                     /locus_tag="PLG52_09880"
     CDS             complement(2249..>2864)
                     /locus_tag="PLG52_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNB41797.1"
                     /translation="LVDGLMRILISLFASPIIGFVFGFLFLRLVLELCVDAAPRVNEF
                     FKKSQLLTALALGLSHGSNDGQKTMGVITLALVTGGYIASFSVPIWVMAACACALALG
                     TAVGGWSLLRKPTRAYFKIRPVDGFSTQVTSALVIITASLTGGPVSATQVINTAIMGV
                     GTAERANKVRWGTARDIITAWILTIPATAIFAAGIYWVAIRYVF"
BASE COUNT          685 a          714 c          738 g          727 t
ORIGIN      
        1 gcgaatggca acactcggat ggtcgggtcc gaaggtggct tcgtcgatct tcaaggcgcg
       61 ttcgaaggcg gctttcgccc cggcatgatc ccccaagtct ttcagcacca tgcccagatt
      121 gttgacatcg attgctacgt ccggatggtc gggtccgaag gtggcttcat cgatcttcaa
      181 agcgcgttcg aaggcggctt tcgccccggc atgatccccc aaggctttca gcacaccacc
      241 cagattgttg atgagcgtcg caacatttgg atgatcttca ccaagaacat tctcaaaaat
      301 cttcaaagcg cgttcgaagg cggctttcgc cccggcatga tctgctaaat ctttgatatg
      361 ataaccaatg ctattccata attcaccggc aggttcgagg gctgcagctt ctgcatattc
      421 agcaacaacc cttaaatggg gaaggagagg agtgtaaaga gcatagttcc cggtttggtc
      481 ttcatctgaa ttggtttggt tagctaggtt tgcaagggct tcactgaaag gttttagtaa
      541 atgattgtct tcatccaaac cgcgcgcaaa ttccgccagc aaggggtgaa tggaatgtcc
      601 ttccttgagc aagcccaagc cggtcagctc gcttatggct tcgtcacagg cgtcggtggg
      661 atcgcccagc gctgcatcta gtatggcttg tggaatgggg gtattaggag cgcaatagcc
      721 caaggtagtg aataactttt gtgccgtttc atcttttaca aaaatccatg attgggcaaa
      781 ggtttgtgcc aaactcaaat cgtgcccggt caggcttttt tgttccactt tccaattttg
      841 cattgattta tgtgaaagag cgttttttgt ctgctcaagg tattcttgaa tcttcaagcg
      901 tggctgtttt tcaagataac gccctgccaa ttccaatgcc aacggaagat agccaaggtg
      961 ttctgcaagt ttttcaagcg ccacatcgtt ttcgcgttcc ggcgcgatgt acttgcgtaa
     1021 aaattcaagg ctttctgttg agctgaactc gcccaagggc aggcgtttca gtcccagccc
     1081 ggcgctccaa ttcgtatggc gagaagtaat gagcaccttc aaattggagt gatgtaagac
     1141 acccagaatc tcattcgcga cgttgtactc ttcaaagtta tcgaggatca acaagcgtgg
     1201 tccgttcatt ttccattcgt ggatagtgac cgcaacttgc tccggctgag tcggcgggaa
     1261 gttcggcaga ttcatcttcg agccgcataa tgcaatttgt gactcaatat tttgtacctc
     1321 gcgcacatcc agccaatgca cacccttgaa ttgatagccg tggcgataag caaactccac
     1381 tgctagttgc gtcttgccga gtccgcccat gccggtgatg gtttggttga tgactgcgcc
     1441 agtgtcattg ctatcaagca gagattgaac taatgtctcc aaatctgcta cacggccggt
     1501 gaagatcgca ttacgaggga acggcagata ggagcccagc ggaaggtcgt gatttgcaga
     1561 tggggcaggg gaggggcttg agttggatgc tgtccatgat ggtcccattc gctggttaag
     1621 tgcatcatat aattgctgga aatctgagtc tcgcaaaatg tcagggctca acgtattcat
     1681 atttttgatg acagtgctca aacggtcaat ctcatcacgg aaacggtcgt aatcatccag
     1741 ttcttcagaa atgcccggta tattagccag ttctttcaag cttcgcatct tttggtcaag
     1801 ttcactcttt ttcttttccc aatatgtgag gtaatcaatg cggtctaggg catcatagat
     1861 tttcgcgtct gagagaacaa ttgggaagat gcggtcggcg aagtctttgt ttgcggcgat
     1921 ctcaaccaac tcgaacatgc aatttttgga gcgcaagtat ttgtcactaa tgacgacgat
     1981 gacacaattt ccttccccga tccgccgcat gaactcgcgg atcatgcctt tgtagcccaa
     2041 gtcgcgtttg tcgcgcacaa tttttatccc acgcttttgc agggattggt caagtcgatt
     2101 aacaatggct tcgcgttcgt ctgtttcctc gccccatgca tacgagataa aaattgaatt
     2161 ttcgtagtcc gacatcaaaa tgcctccaca atcgctgttc tacgatgatt ttggagtata
     2221 gaggcaaagg cttgattttg caagaagttt agaaaacgta gcgtatcgcc acccaataaa
     2281 tgcctgccgc aaaaatagcc gtggcgggga tggtgagaat ccatgctgtg atgatgtctc
     2341 gcgccgtacc ccaacgcact ttgttggcgc gttctgccgt gccgacgccc atgatggcgg
     2401 tattgatcac ctgcgtggcg ctgacgggtc cgcccgtgag ggaggcggtg atgatgacca
     2461 aggcagaagt cacttgggtc gagaaaccgt ccacagggcg aattttgaaa taagcccgtg
     2521 tcggcttgcg caacaagctc cacccgccga cggcagtccc taaggcgagg gcacaggcac
     2581 aggctgccat cacccaaatc ggcacgctga aagaggcgat gtatccgcct gtgacgagcg
     2641 ccaaggtgat cacgcccatg gtcttctgac cgtcattgct gccatggcta agtcccaacg
     2701 caagggcggt gagcaactga ctctttttga aaaattcatt cacgcgcggc gcagcgtcca
     2761 cgcacagttc cagcaccagc cgcagaaaaa gaaaaccaaa cacaaagccg atgatggggg
     2821 aggcgaagag cgagatcagg atgcgcatca aaccgtccac caag
//
LOCUS       DAOQNJ010000351         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25660_length_2859_cov_9.420827, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000351 DAOQNJ010000000
VERSION     DAOQNJ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25660_length_2859_cov_9.420827"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PLG52_09885"
     CDS             <1..785
                     /locus_tag="PLG52_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB41798.1"
                     /translation="AERPMLGIVPGDFSPEQAKALNVPVLEGMRLDATVEGMGAKRAG
                     IERDDVLVSMAGKPITNDFTSLQLAIAGKKGGDVIEVEFYRGAEKKKVSMELTKRPMA
                     DVPFNSVELAKKARALMEPALAEIEKCFEGYSDEQAMKRPEPNEWSALEVVAHLIQGE
                     RFNVIYLASLVDGYEAVSDGFGSNISESMTAMVKANPSIKLMMEALRRSVDEILLFVE
                     MLPADFVANKGSYYRFGSGLLQPEFHLNAHTDQLRKALEAAK"
     gene            914..1399
                     /locus_tag="PLG52_09890"
     CDS             914..1399
                     /locus_tag="PLG52_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41799.1"
                     /translation="MKQNIKKLSVLAIILMLTLSACKANVSRNDDGSLSVETSVTQAQ
                     LQEIISSSIADPLIKSLTVTLQQGYVSVIGERERLNDASKTDTMSFRLDLGVSNGELT
                     ASISDAQIDGVSIEQNRVDHWNQTIANRIANIGSRNENAQLQSVSITPEQVLMTWTVV
                     R"
     gene            complement(1440..2666)
                     /locus_tag="PLG52_09895"
     CDS             complement(1440..2666)
                     /locus_tag="PLG52_09895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB41800.1"
                     /translation="MSLEQFEIQKRNFRYVLIDGVGVSIANVAAPFLPVLLTRLGASN
                     FQVGLLTSMPGVTGLILAIVVGRFLQSRSNIVPWYGLSRLLVIACYTLTGIIAFTVSG
                     QAAVIAILVIWGLATIPQTGLAVAFSVVMNAVAGPEGRYALLSRRWAIFGLTGVVVTF
                     FITRVIDRVDFPLNYGLMFMGLSLGGLISYYFSRKIELPPQTPPAPVPSSSAFDSARS
                     YFSLIRSEPAFQSFTVKKFVYTSAIALGQPILPLFFVREVHATDAQIGTITMVMTMVM
                     LVGYFLWPWMSRKYSGRVVLLATTLGLAFYPALTAATVKVEWIIVYAGIAGLFQAGLD
                     LVFFDELMKTVPPEYSATFVSFSQSLQYFSAVIAPTVGTWFADSIGLGGALWVSAGLR
                     LLGFVLFLIPDRRKVT"
BASE COUNT          864 a          670 c          710 g          615 t
ORIGIN      
        1 ttgcagaacg cccgatgttg ggaattgtgc caggcgactt tagcccagaa caagcaaagg
       61 ctttaaacgt ccctgtgctt gaagggatgc gacttgatgc cactgtggaa ggaatgggcg
      121 caaaacgagc agggattgag cgagatgatg tgcttgtgag catggcaggc aagcccatca
      181 ccaatgattt caccagcctg caacttgcca ttgccgggaa aaaaggcggg gatgtaattg
      241 aagttgagtt ttatcgtggc gcagaaaaga aaaaggtttc catggaattg accaaacgtc
      301 ccatggcaga tgtgccgttc aactcagttg agttggcaaa gaaagcccgt gcgctgatgg
      361 aacccgccct ggcagagatc gagaaatgtt ttgaagggta ttcagatgaa caagccatga
      421 aacgacccga gccaaacgaa tggagcgcac tggaagtggt ggcgcacctc attcaaggtg
      481 agcgattcaa tgtcatttat cttgccagct tggtcgatgg gtatgaagcc gtgtctgatg
      541 ggtttgggag caatatcagt gaatcgatga cagcaatggt gaaagccaac ccgtccatca
      601 aactgatgat ggaggcttta cgccgcagtg tggatgagat tctgctcttc gtagaaatgc
      661 tcccagcaga ttttgtggcg aacaaaggca gttactaccg ctttggcagt gggttgttgc
      721 aaccagaatt tcatttgaat gcccatactg accaacttcg gaaagcattg gaagccgcga
      781 aataagtttt tactgtttat aaaataaaaa gccgagtctc tcaaagactc ggcttttttt
      841 acagagaatt tacaaactaa tattgaacag aaaacaaagg agtgggatac tacaaaacat
      901 tggagaataa aacatgaaac aaaatatcaa aaaattatct gtccttgcca taatcttgat
      961 gcttacattg agcgcttgca aagccaatgt gtctcgtaac gatgatggct ctttatctgt
     1021 tgaaacttct gtaacacaag cccagttgca ggagattatt tcttcttcca ttgcagaccc
     1081 acttatcaag agtctgaccg tgacgctaca acagggatat gtttcggtga ttggcgaacg
     1141 cgaacgtttg aacgacgcca gcaagaccga cacgatgagt ttcaggctcg acctcggcgt
     1201 gagcaatggc gaattaaccg cctccatttc agatgcacaa attgacggag tgtccattga
     1261 gcaaaaccgc gttgatcatt ggaatcagac cattgccaac cgcatcgcaa acattggcag
     1321 ccgaaatgaa aatgcccaat tacagtctgt atcgatcact cccgaacaag tcttaatgac
     1381 ttggactgtt gtccgataat aaaaagaagt ccatgagatg aactcatgga cttctttttt
     1441 taagtcactt tgcgccgatc aggtattaga aagagcacaa accctagcaa tcgcagacca
     1501 gcactcaccc acagcgcacc acccaacccg atagaatccg cgaaccaggt accgaccgtc
     1561 ggagcgatga cagcggagaa atattgaagg gactgagaaa aagatacaaa ggtggcgcta
     1621 tattccggcg ggacggtttt catcagctca tcgaagaaga ccaaatccaa accggcttga
     1681 aagagcccgg caatgcccgc atatacaatg atccactcca ctttaactgt ggcagcggtc
     1741 aaagcggggt aaaacgccag ccctaatgtg gtggcaagca ataccacccg tccgctatac
     1801 ttgcgagaca tccacggcca gaggaagtaa cctaccagca tcaccatagt catcaccatg
     1861 gtgatcgtgc cgatctgcgc gtcagtcgca tggacttcgc gcacgaagaa caggggcaag
     1921 atgggctgtc ccagtgcgat ggcagaggtg tacacaaact ttttgacggt aaaggattga
     1981 aaggcaggct cactgcgaat gagggagaaa taactccgag ctgagtcaaa agctgatgaa
     2041 gaaggaacag gagcgggcgg agtttgaggc ggaagttcta tcttgcgtga aaagtaataa
     2101 gaaatcagtc cgcctaacga aagccccata aacatcaagc cataattgag agggaaatca
     2161 acccggtcga tcacacgcgt gatgaagaac gttacgacca cacctgttag accaaagatc
     2221 gcccaacgcc ggctgagcaa agcatagcgc ccttcaggtc ctgccactgc attcatcacc
     2281 acagagaaag caacggctaa accggtttgt ggaattgttg ccaagcccca gatcacaagg
     2341 atggcaatca ctgccgcttg ccccgaaacg gtaaaagcaa tgatgcctgt cagggtgtaa
     2401 catgcgatca caagcaaacg agatagccca taccacggca caatattgct acggctttgc
     2461 aaaaaacgcc ccaccacaat ggcaaggatc aaacccgtta ccccaggcat cgaggttagc
     2521 aagcccacct gaaaattaga tgctcccaag cgagtaagca gcacaggcaa aaatggcgcg
     2581 gcgacatttg caatactcac gcccacgccg tcgatcaata catagcgaaa gttgcgtttt
     2641 tggatttcaa attgttcgag agacatagga aaaatgggtg agacagaatt tcgattataa
     2701 cgctcccctc catggataca atggaaacaa atacacattg ggcggcgtcc gtgtgtaaag
     2761 gagaaatcat gaaaaaaatt gtgatgttgt tttcgctttt taccatcctg ctctctgctt
     2821 gcagcagtgg aagttcagcc ggggtggcag agtctggtc
//
LOCUS       DAOQNJ010000352         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25693_length_2857_cov_6.207352, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000352 DAOQNJ010000000
VERSION     DAOQNJ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25693_length_2857_cov_6.207352"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLG52_09900"
     CDS             complement(<1..228)
                     /locus_tag="PLG52_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41801.1"
                     /translation="MRKPQTPPFVFKNIYPWFRYILLFGRKLHQVLMDLQIAFSKRPA
                     SSPVLPDAYTAFNTSTVDSEIMLQRLEGEMPA"
     gene            complement(411..1385)
                     /locus_tag="PLG52_09905"
     CDS             complement(411..1385)
                     /locus_tag="PLG52_09905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB41802.1"
                     /translation="MKLPEELQFIENARMPQKTTTARLDGKVCIITGATSGVGYETAK
                     RIAQAGAHLVLVNRNAEKAESLKAELTALSNARVDLVHADFSNLDDVQSAASQILHQF
                     PAIHVLINNAGMHMTTRKLTVDGFETAFAVNHLASFLFTRLLLDRMIASAPARIIQVN
                     SEGHRFGGLKLDDLNWDKRRYKGLQGYGASKTAQLMTVWELAERLNGTGVTINAVHPG
                     EVATNIGMNNGLLYRWYQKYLLAHILKDPKISGEALYYFAAAPEVSNVSGNFFNLTIE
                     EKPAAHALDRVMGKRVWDISEELTGLHHKMMGNTASTAVENPGFTAAS"
     gene            complement(1397..1837)
                     /locus_tag="PLG52_09910"
     CDS             complement(1397..1837)
                     /locus_tag="PLG52_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB41803.1"
                     /translation="MSIAPLTTDDILRLWSQTYNREGKPDWSHIFPYYHDAIVFQDSI
                     QRVEGKENFIALCNRLTQRCEQLHMDISSIAQKKNEIFMEWKMTMIFKKFPSTPMFGC
                     TKLTFGEDNRIINQRDYYDLWGDIFNEIPWFHRPYRKFMHKYFG"
     gene            2355..>2857
                     /locus_tag="PLG52_09915"
     CDS             2355..>2857
                     /locus_tag="PLG52_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41804.1"
                     /translation="MTIYSGLRIAFGKDQPHVQFTVEMDPPSVYWVYRIKSSQIENLI
                     QKLRIPPNLSLTPVRCLDIDEPSYYLALNAYRVSGLVSGVRAEWSVFVRDSTNTARYM
                     IVDARSSTTSMDPVSIVTKKSTVLHKREGNVIRTQIGDGADAFVSTITLPEQSPSVHS
                     SAEWVTA"
BASE COUNT          679 a          655 c          742 g          781 t
ORIGIN      
        1 ggcaggcatt tcgccttcca gacgctgcaa cattatttca gagtcaaccg ttgacgtgtt
       61 gaaggcagta taggcatctg gtagaacagg agaagaagct gggcgcttgc tgaacgcaat
      121 ttgcaggtcc attaagacct gatggagttt gcgcccaaaa agtaatatgt atcgaaacca
      181 cgggtaaata ttcttgaaaa caaagggagg cgtttgtggt ttgcgcatgt gggtattaaa
      241 tagttgtcga gtgctttcta aaaaccatcc cgtcattgct tcgggtggtg ttcttccgcc
      301 cgacacttgc gccgccttgc cagtgcggtg agcaatctca tattctgact atgggattgc
      361 ttcgccgcaa agaacaagag cgcggctcgc aatgacggaa atctcattac ttatgaagcg
      421 gcggtaaagc cagggttttc cactgcagtg gaagctgtat tgcccatcat cttgtggtgc
      481 aagccggtta attcctcgct gatatcccat acacgtttgc ccattacccg atccaatgca
      541 tgcgctgctg gtttttcttc gatggtcagg ttgaagaaat ttccacttac gttgcttact
      601 tctggggcag cggcaaagta gtagagcgct tcgccggaga tttttgggtc ttttaggata
      661 tgggcgagca ggtatttttg ataccaacga taaaggagtc cattattcat gccgatattc
      721 gtggcgacct cacctgggtg gactgcattg atcgtcacgc cagttccgtt cagccgctct
      781 gccaattccc aaacggtcat taactgggcg gtcttggatg caccatagcc ttgcagacct
      841 ttgtagcggc gtttatccca attgagatca tccaacttca agccgccaaa gcgatgccct
      901 tctgagttga cttgaataat gcgggcaggt gcggaggcga tcattcggtc tagcaataag
      961 cgtgtaaaaa gaaaggacgc aaggtgattg acagcaaatg cagtttcgaa accatcgact
     1021 gtcagtttgc gggtggtcat atgcatgcct gcgttgttga tcaagacatg aatggcaggg
     1081 aactgatgca ggatctggct cgcggcactt tgaacatcgt cgaggttgga aaaatcagca
     1141 tggacgaggt caactctggc gttggaaaga gccgtgagtt ccgctttgag tgattctgct
     1201 ttttctgcat tccggttcac caacaccaga tgagccccgg cttgcgcgat ccgctttgcg
     1261 gtttcatagc ccacgccgga ggttgcgccg gtgataatgc acactttgcc atccaaccgg
     1321 gcggtggtgg tcttttgcgg catgcgtgcg ttttcaataa attgaagttc ttcagggagt
     1381 ttcatttgta aaccgtctat ccaaaatatt tgtgcatgaa ttttctgtag ggacgatgga
     1441 accaggggat ttcattgaag atatcgcccc agagatcgta gtaatcgcgt tgattgatga
     1501 tgcgattgtc ttcgccaaaa gtgagcttgg tgcagccgaa catgggcgtg ctgggaaact
     1561 tcttgaagat catggtcatc ttccattcca tgaagatctc attctttttt tgggcaatag
     1621 atgaaatatc catgtggagt tgttcgcagc gctgcgtgag ccgattgcat aaggcaatga
     1681 aattttcctt gccttccacg cgttggatgc tgtcttgaaa cacgatggca tcatggtaat
     1741 aagggaaaat atgtgaccaa tcgggcttgc cctcgcgatt gtaggtctgt gaccaaagtc
     1801 ttaagatgtc atctgtggtt aggggggcga tgctcataaa ctccttttat tggaaggaca
     1861 aattatttga tattgatgtt acgcggtagg ggtgacccaa ccacattttg tggatacctt
     1921 atgcaaaggg cgaccctttt ttgtaggctg gcatacggtt aaaccctgac gggtcgcctc
     1981 tacgccgaaa tcgcggaata taaggtatgg atttaactgg gcgacgcatc taaagtcgca
     2041 cgaatgtgta atagtctata ctttttaagt gtactatatt tgtcggaatg ccaaatataa
     2101 ttaacgacat tcttaggaat aattcttaac tcaccttacg ccgtaggcgc gacccaaccg
     2161 cgttcttggt aatttttttg gcaaagggcg accctttctt atcgatttgg gttttggcta
     2221 gaccctgacg ggtcgcccct acacattaag accaattttg gagtcaattc atggagaacc
     2281 ccgtattcgc aaaaaaactg tctaagctag ggaggcttgg gcgaagcatt ctgaaacgat
     2341 ttatgtactc tcaaatgacg atctactccg ggcttaggat cgcgtttggg aaagatcaac
     2401 cgcatgtaca gttcaccgtt gaaatggacc cgccttcggt ctattgggtg taccgaatta
     2461 agtcatctca aattgagaac ctgattcaaa aattgcgcat cccgcccaat ctctcgttga
     2521 ctcctgttcg ctgtctcgac atcgatgagc catcgtatta tctggcgctc aatgcgtatc
     2581 gcgtatctgg cttggtgagc ggcgttcgag cggagtggtc ggtgttcgtg cgcgattcta
     2641 cgaacacggc tcgttatatg atcgtggatg cgcgttcctc cacaacctcc atggacccgg
     2701 tcagcatcgt tacgaaaaaa tctacagtgt tgcacaagcg cgaagggaac gtgatccgca
     2761 cccagatcgg ggacggcgcc gatgcatttg tctccaccat caccttgccg gagcaatcgc
     2821 cttcggttca ttcctcggcg gagtgggtga ctgccaa
//
LOCUS       DAOQNJ010000353         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25910_length_2844_cov_10.764073, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000353 DAOQNJ010000000
VERSION     DAOQNJ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25910_length_2844_cov_10.764073"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..509)
                     /locus_tag="PLG52_09920"
     CDS             complement(36..509)
                     /locus_tag="PLG52_09920"
                     /inference="COORDINATES: protein motif:HMM:NF018738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HNB41805.1"
                     /translation="MGLFSKLKKGLGIGTAKVELVDVPTQINRNTQEVSGKVTITAKS
                     DQKVLSVRVAVVEISNEEFNGQQREMKKDLAFAKLDGFDLKTDETRTLPFTIALVAGD
                     KVSVNIFGGTLEISGGPSLSSSNSIFSPVKYQIVAVADCEGVAIDPTDAKPLTIL"
     gene            complement(677..1690)
                     /locus_tag="PLG52_09925"
     CDS             complement(677..1690)
                     /locus_tag="PLG52_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB41806.1"
                     /translation="MTVKQVLITGACGEIGQALVQELAKKGGYRIVTSDMAPLPDSIK
                     NLVAEHVQGDLVYKVKVLYDYDFDVIFHLAASLSSKAEIATEEAHRINVEGTMQLLML
                     AAYRSEKYGKSVKFLFPSSIAAYGMPTLEAKQGAGAVKEEDWDYPHTMYGCNKLYCEK
                     LGMYYSKFHGQKHLDDNPPIMLDFRAIRFPGLISAFTLPSGGTSDYGPEMLHAAAQGK
                     DYACFVRPDTKISFMAMPDAIKSLLMMLDAPREKLTTNVYNVAAFAITAEDFRQRALK
                     AFPQANITFAPNPRRQGIVDSWPEDVDDTRARADWNWKPDYDVDRFFEEYFLPEIRKR
                     YGK"
     gene            1768..2556
                     /locus_tag="PLG52_09930"
     CDS             1768..2556
                     /locus_tag="PLG52_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41807.1"
                     /translation="MTFKRILRSGLLALLLTSCSALDEMLATNTPSPATETPAPTSTI
                     IWFPPSATPTLQFLSTKAPTPEMRPGLSNELLRDDFSSPFDWDVVTSNDASAVMQDNR
                     LTLAVQSQVYMTSLRHDTLLTDYYAEITAQPSLCKGEDSYGFLIRANGGSYYRFALAC
                     DGTVRAERVSNSVRLVLQKPIPSGDVPPGAPGNVRIGVWIVGNEMRLFLNGRFQFSIN
                     DPSFPIGTVGVFVRSAGETAAIVSFSDLVIQEVDYIPPTPTATP"
BASE COUNT          728 a          771 c          590 g          755 t
ORIGIN      
        1 tagaagggat ttttctaaag ataaaaatga aactactaca aaatcgttag aggcttggca
       61 tcggtggggt caatggcaac gccttcgcaa tctgcaacgg ctacgatttg atacttcacc
      121 ggcgagaaga tggaattgga actggataaa ctcggtccgc ctgaaatttc caaggtgcca
      181 ccgaaaatgt tcacagatac cttgtcccct gccaccaaag cgatagtaaa aggcaaagta
      241 cgggtttcgt ctgtctttag gtcaaatcca tccaactttg cgaaagcaag gtctttcttc
      301 atttcgcgct gctgcccgtt aaattcttca tttgatattt ccaccactgc cacgcgaaca
      361 gacaaaacct tctggtcgct cttggctgta atggtcactt ttcctgaaac ttcttgcgtg
      421 ttcctgttga tctgagttgg cacatcaacc aactccactt ttgccgtacc aatccctaaa
      481 cctttcttga gcttgctaaa aagacccata atgacgttac tcctttggaa aatatataca
      541 gaataaaaaa cttattccat agtgaacgta tgatagaggt ttaaaaacag aatgacaaga
      601 tagaaaccat ttacaatcta caaacaaaag actgtgcaaa tttgcacagt cttttgtttg
      661 ttataagcag ttttctttac tttccgtatc gctttcgaat ctctggcaag aaatactctt
      721 caaagaaacg gtccacatcg taatcgggct tccagttcca atcagcgcgg gcgcgagtat
      781 cgtccacatc ttcgggccac gagtcgacga tgccttggcg gcgcggattc ggcgcaaagg
      841 taatgttcgc ttgcgggaac gccttcaatg cacgctggcg gaaatcttca gccgtaatag
      901 caaacgctgc cacgttatat acattcgtag tcaatttttc acgtggtgcg tccaacatca
      961 tcaacaatga tttgatggcg tcgggcattg ccataaagga aattttggta tcggggcgca
     1021 cgaaacaggc atagtcttta ccctgagccg ccgcatgtaa catttcggga ccataatcac
     1081 tcgtgccgcc gctcggcaag gtaaaggcgc tgatcaagcc tgggaagcgg atcgctcgaa
     1141 agtccagcat gatcggtggg ttatcatcca aatgtttttg accgtgaaat ttgctgtaat
     1201 acatccccaa cttttcgcaa tacaacttat tacaaccata catcgtgtgc ggataatccc
     1261 aatcttcttc tttcaccgca cctgcgcctt gctttgcttc aagcgttggc ataccatatg
     1321 cggcgatcga acttgggaac aagaacttca ccgacttgcc atatttttct gaacgatatg
     1381 ccgccaacat cagcagttgc atcgtccctt ccacattgat gcgatgtgct tcctccgttg
     1441 caatctctgc ttttgaagaa agcgacgccg ccaaatggaa gatcacatca aaatcataat
     1501 catagagaac ttttacttta taaaccaaat cgccttgcac atgctctgcc actaaatttt
     1561 tgatcgaatc tggcaaaggc gccatatccg atgtcacaat tcggtaaccg cccttcttcg
     1621 ccaattcttg caccagcgcc tgaccaattt ctccacacgc accagttatc agcacttgct
     1681 tcacggtcat cttcatctcc tgatatgcta gaatgttgta taaatatttt acgacacatt
     1741 gaaacgaaac gctgtcataa aaacccaatg actttcaaac gaattttacg ttcagggctt
     1801 ctcgctctct tattaacctc ctgctctgcg ttggatgaga tgctcgccac caacaccccc
     1861 tccccagcga cggaaacccc ggctcccacc tcgaccatta tctggttccc tccatctgca
     1921 acacctacgc ttcaattttt atccaccaaa gcccccaccc cagagatgcg ccccggtctc
     1981 agcaatgagc ttcttcgtga cgatttctcc agcccgttcg attgggatgt ggtgacatcc
     2041 aacgacgcca gtgcggtgat gcaagacaac cgtctcactc ttgccgtgca aagccaagtc
     2101 tatatgacca gcttgcgcca cgacacgtta ctcaccgatt attatgccga gatcacagca
     2161 caacctagct tatgcaaagg cgaagatagc tatggctttc tcattcgtgc caatggtggc
     2221 tcctactatc gtttcgccct cgcctgcgat gggaccgtcc gtgctgaacg tgtgagcaat
     2281 agcgtaaggc ttgtgctgca aaaacccatc cccagcggag atgtgccccc cggcgcaccg
     2341 ggcaatgttc gcattggagt atggatcgtt ggcaatgaaa tgcgcctctt cctaaacgga
     2401 cgtttccaat tcagcatcaa cgacccgtcg ttccctattg gtaccgtcgg cgtattcgtc
     2461 cgctcggcag gagaaaccgc cgccatcgtt tcgttttctg accttgtcat tcaagaggtg
     2521 gattacattc cccctacccc aactgctaca ccgtagcaat tgacgatttt tgatttgaga
     2581 tttacgatta gaacagtagt gggaaacgca tcaaacatca acaccatacc cacaacacat
     2641 attggtactt ggaatactgg tcactgatac tatgctatcg tcttccgact tcctacacct
     2701 tccttacacg ccagacctca cagaggcagg cattgcctat gcattgaggt cactggcata
     2761 ttcttatgag cgcgatgggc gttctccata tgggcgtttg cgccgtctgg tggcaaatgt
     2821 tgccgtagag ttggcatttc ggcg
//
LOCUS       DAOQNJ010000354         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25911_length_2844_cov_9.855145, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000354 DAOQNJ010000000
VERSION     DAOQNJ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25911_length_2844_cov_9.855145"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PLG52_09935"
     CDS             complement(<1..647)
                     /locus_tag="PLG52_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNB41808.1"
                     /translation="MRLIRILLLGIFIFSLVPGIVSAQTPTPPTAVRVILNSMTPEER
                     VGQLFLVTFKGTNTGTDSQIYELITKRHVGGVVLTAGNDNFSETDTLARAHQLIEDLQ
                     QVEWTNSANTAVDPVTGSLERYVYVPLFVGISQEGDGYPTDQILNGLTPLPSEMAIGA
                     TWNTEFAQNVGEVRGQEIAALGFNIFLGPSLDVLETPSSSGGDLGTRVFGGDPFWV"
     gene            complement(766..1518)
                     /locus_tag="PLG52_09940"
     CDS             complement(766..1518)
                     /locus_tag="PLG52_09940"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNB41809.1"
                     /translation="MLETFPRPILLAHRGDLAKAPENTLPAFSQAIQKGADGVELDAK
                     LTIDGHVIVIHDETVDRTTDGKGKVATFSLEAIRKLDAGSWFDSKFADTNVPLLEEVF
                     ETVGKDKLINIELTNYYTPRDGLVVKVCELIKRHNNAKQIIFSSFLPSNLKIAQQTLP
                     EIPRGLLALPGFMGLWARSFGFMFGDYQASHPHISDANRESIQRAHRVNRRVHVWTAN
                     SPQEIQRLRDWGVDGLFTDDPGTAVQALGRTR"
     gene            complement(1555..>2844)
                     /locus_tag="PLG52_09945"
     CDS             complement(1555..>2844)
                     /locus_tag="PLG52_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB41810.1"
                     /translation="GGFTPPQINSLEDTGLSTLWLQDLILKVLYFRGYLNGSKISDEI
                     ALPFAGVTDQLLTTLKQEKLIEVKSSAGGLGEGSYTYGITSAGIVRAREALERSQYAG
                     PAPVPFEVYNEAIRKQKAGRVTVTTRTMRQILSQLIISESTFQRLGPALNSGTSIFMY
                     GPPGNGKTSIARAFGNLVLSQNMYIPYALYLDGQVIKVYDAVSHSLAPDNDVASNPTG
                     SLRSNTRRDPRWVKIRRPFIVVGGELTLEGLDLVFDDTTKFYEAPFQVKANGGILLID
                     DFGRQQVRPRDLLNRWIVPLENRMDFLRLHTGRKVEVPFDVLIVFSTNLPPKDLVDEA
                     FLRRLRHKIEIGDPSFEEYREIFKRVAQDKRIEYNDQGLAYLLQEWYIKRNRKLRASH
                     PRDLCDQILDISSYMAVPPSMSREMLDRAAKAYFVDI"
BASE COUNT          772 a          688 c          683 g          701 t
ORIGIN      
        1 acccaaaacg ggtcaccgcc aaacacacgc gttcccaaat caccgcccga tgaagatggt
       61 gtctctaaca catccagcga tggaccaagg aaaatattga aacctaacgc agctatttcc
      121 tgaccgcgaa cttctccaac attttgcgca aattccgtgt tccatgttgc cccaattgcc
      181 atttcactag gcaaaggcgt taacccattc agaatttggt ctgttgggta gccatcccct
      241 tcctgtgaaa taccaacaaa caaaggcaca tacacatatc gttcaagaga accggtaacc
      301 gggtctacag cggtgttcgc tgagttggtc cactccacct gctgaaggtc ttcgatcaac
      361 tgatgcgcac gtgccagagt atccgtctcg ctaaagttat catttccggc tgtaagcacc
      421 acgccgccca catgccgctt ggtgatcagc tcataaatct gtgaatctgt cccggtattc
      481 gtacctttaa acgtaaccaa aaataactgc ccaacccgct cttcaggcgt catcgaattt
      541 aaaataactc gcaccgcagt aggcggtgta ggcgtttgtg cggaaactat ccccggcaca
      601 agcgagaaga taaaaatacc caagagaaga atacgaatta agcgcattgc cccattctta
      661 taacacaact tgctattaag acaaaatgga cgccgttcat tgaacgacgc cattggaact
      721 tcgcttcaaa ccgcgctcgg gcttgctccc ggcattcgga aagtctcacc tcgtccgtcc
      781 aagcgcttga actgctgttc cggggtcatc tgtaaagagc ccatccacgc cccaatcgcg
      841 caatcgttga atttcttgcg gagagtttgc cgtccacaca tgcacacggc ggttgacacg
      901 atgtgcccgt tgaatactct cgcggttcgc atcagaaata tgtggatgag aggcttgata
      961 atcgccaaac ataaagccga atgaacgcgc ccataatccc ataaaaccgg gcaatgccaa
     1021 aagtccgcgc ggaatttcag ggagagtctg ttgtgcgatc tttagattcg aagggagaaa
     1081 agacgaaaaa ataatctgtt tggcgttgtt atgacgctta attaattcac agacctttac
     1141 cactaaccca tctcgcgggg tgtaataatt cgtcaattcg atattgatga gtttatcttt
     1201 tccgacggtt tcaaagactt cttccaacaa aggtacgttt gtatctgcaa actttgagtc
     1261 gaaccatgaa cctgcatcta atttacgaat ggcttcaaga gagaacgtag ctactttccc
     1321 ttttccatct gtagtgcggt caaccgtctc gtcgtgaatg acaattacat gaccatctat
     1381 ggttaattta gcgtccagtt ctacgccgtc ggcgcctttt tgaatagctt gagagaacgc
     1441 cggcagggtg ttctctggcg ctttagcaag gtcaccgcga tgggcaagaa ggatagggcg
     1501 agggaaagtt tcaagcatgg taggcaggct caagatccga aagaggcgtt gggtttaaat
     1561 atccacaaaa taagcttttg cggctcggtc tagcatttca cgagacatcg aaggcggcac
     1621 agccatatag gatgaaatat cgagaatctg atcgcacaaa tcacgcggat gagaggcgcg
     1681 aagcttgcga ttacgtttga tataccattc ttgcagaaga tatgccaagc cttgatcgtt
     1741 gtattcaatg cgcttatctt gggctactcg tttgaaaatt tcacggtatt cttcaaacga
     1801 aggatcgcca atttcaatct tgtggcgcaa gcggcgaagg aacgcttcat ctaccaagtc
     1861 ttttggcgga aggttagttg agaagacgat taaaacgtcg aagggcactt ccactttgcg
     1921 tccggtatgg aggcggagaa aatccatgcg gttttcgagc ggaacgatcc aacggtttag
     1981 caaatcacgc gggcgcactt gttgacgtcc aaagtcatcg atcagcaaaa tgccaccgtt
     2041 ggcttttact tggaatggcg cttcataaaa tttggtggtg tcatcgaaaa caaggtcgag
     2101 accttcgaga gtcaactcgc cacctactac aatgaaaggg cggcgaattt ttacccaacg
     2161 tgggtcacga cgagtgttag aacgcaaaga acctgttggg ttggatgcca cgtcattatc
     2221 aggagcaaga gaatggctga ctgcatcgta aactttaatc acctgcccat ccagatagag
     2281 tgcgtatgga atgtacatat tctggctaag gacaaggttg ccaaaagcac gcgcaatact
     2341 ggttttccca ttgccgggag gtccgtacat gaaaatggaa gtaccagagt ttagagcggg
     2401 tccgaggcgc tgaaatgtcg attcagaaat gattagctgt gaaaggattt ggcgcatggt
     2461 gcgtgtggta acagtaacac gccctgcttt ttgcttgcga attgcttcat tatagacttc
     2521 gaaaggcaca ggagcaggtc ctgcatattg gctgcgttcc aacgcttcac gcgcgcggac
     2581 gatcccagcg ctggtaatgc catatgtgta agagccttca cccaaaccac cagccgaaga
     2641 tttaacctcg attagttttt cttgtttgag ggtagtgagg agttggtcgg tcacgccagc
     2701 aaatggtaaa gcaatctcat ccgaaatctt gctgccgttc aaatatccgc ggaaatacag
     2761 tacttttagg ataaggtcct gaagccaaag agtagaaagc cctgtatctt caaggctgtt
     2821 gatttgcggg ggcgtaaacc cgcc
//
LOCUS       DAOQNJ010000355         2843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25927_length_2843_cov_7.669656, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000355 DAOQNJ010000000
VERSION     DAOQNJ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25927_length_2843_cov_7.669656"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLG52_09950"
     CDS             <1..562
                     /locus_tag="PLG52_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41811.1"
                     /translation="TKMLRGNIELWLAFLTCILIGAGYGVVTVWTREVPAASELFGHT
                     LGIIGFLLMLMTETFYSIRKRSRSAALGRMSTWLKFHIYMGLVGPFMVLLHSSWKFNG
                     LAGATTLLTAIIVFSGFIGRYIFTRIPRTLDGLEIEGTLSQEALKRARQMMALWHTVH
                     IPIGIALFLAAFVHMGGALYYATFLK"
     gene            572..1759
                     /locus_tag="PLG52_09955"
     CDS             572..1759
                     /locus_tag="PLG52_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNB41812.1"
                     /translation="MNNTNRLGCLTGTGLIAALVTLFLLVGVAFASGSKMFSSGALNA
                     QPGENMGGVTSHADIKECNTCHTAPWESATMADRCLDCHTDIANQMRDVAQLHGSIVQ
                     KSPAIACRDCHKEHRGSTASLTDLGENSFPHDTFGFSLNEHQRMENGDPITCENCHGN
                     DISTFASDSCQTCHSDMDIAFTQAHVLSYGTDCVACHDGVDRFDDFNHNAYAFKLEGE
                     HANVACTKCHLDARSIADLQSAPQDCYSCHRQDDVHNGENGTDCETCHNPSNWEDADF
                     DHSRTNFPLTGGHSNVECNQCHTNGTYKGLSSTCVTCHQDPAFHAGAFGTDCESCHSI
                     NAWSPAQFNLSHPEPRVDEGGSGIHHGGASCRECHPSSVFESSCDSCHSGGFEGGEGG
                     DDD"
     gene            complement(1820..>2843)
                     /locus_tag="PLG52_09960"
     CDS             complement(1820..>2843)
                     /locus_tag="PLG52_09960"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41813.1"
                     /translation="DHIETIDSLRAALETDGHTTVFVHADRELPYSLRKVKPDICFNI
                     AEGLGGDAREAQVPALLEMLAIPYTGSRVLANGISLDKTLTKRIWRDRRLPVAPFQEF
                     QFGDEPLRPELKFPLFVKPAREGTGMGVDMKAIVNNETELRERAEYIINTYQQPALVE
                     TFLPGREFTVGILGRADAKLYSRHPEWYEKDGFHRFPVLELDTGNTEGPWVYTNEAKS
                     KDVGPEGNEGFFCPANIDPELEKKLKHLAFRAHNILNVLDVSRTDIRLDEEGNPRLIE
                     INTLPGLTPNYSDLCLQAAAEGISHTDLVLDILYLAAGRWGLLEPREISTESGKTGPL
                     SYRLGR"
BASE COUNT          677 a          700 c          715 g          751 t
ORIGIN      
        1 aacaaaaatg cttcgtggaa acatagaact ttggctcgct tttcttactt gtatattgat
       61 tggggctggt tatggggtag tgacggtctg gacgcgtgaa gtgcctgcgg cttctgaact
      121 ttttgggcac accttgggca ttatcgggtt tctgctgatg ctgatgacag aaacttttta
      181 tagcatccgc aaacgcagcc gcagtgcggc attgggacgc atgtccactt ggttgaaatt
      241 tcacatctat atggggctgg ttggtccgtt catggtgttg ctccattctt catggaagtt
      301 caatgggttg gcaggtgcta ccaccttact taccgccatc attgtcttta gcggattcat
      361 cgggcgttat atcttcactc gtattccgcg cacgctggat gggcttgaaa tagaaggtac
      421 gctttcacaa gaagccctca aacgtgcccg ccagatgatg gctctttggc atacggttca
      481 cattccaatt gggattgcat tgttccttgc agcctttgtt catatgggtg gtgcattgta
      541 ttacgccaca tttttgaaat aggagacaac catgaataac acaaatcgac ttggttgtct
      601 taccggcaca ggcttgatag ccgcactggt cactctattt ttgcttgtgg gtgttgcgtt
      661 tgccagcggg tcaaaaatgt ttagctccgg cgcactcaat gcacaacccg gcgaaaacat
      721 gggcggcgtc acatcgcatg ccgatatcaa agaatgcaac acctgtcata ccgcgccatg
      781 ggaatctgcc acaatggcag accgttgttt agactgtcac accgatattg caaaccaaat
      841 gcgagatgtc gctcaactgc acggcagcat tgtacagaaa tcgcccgcca ttgcatgtcg
      901 cgattgtcac aaagagcatc gcggttcaac cgcatcgctg acagatctgg gcgaaaactc
      961 ttttcctcac gatacatttg gattctcatt aaatgaacat cagcggatgg aaaacggcga
     1021 cccgattact tgcgaaaatt gccatggcaa cgatatttcg accttcgcct ctgactcctg
     1081 ccagacctgc cacagcgaca tggacattgc cttcacccaa gctcatgtgc tctcttacgg
     1141 caccgactgt gtagcgtgtc atgatggcgt agatcgcttc gatgatttca atcacaatgc
     1201 ttatgctttc aagctagaag gtgaacatgc taatgtcgct tgtacaaaat gtcatctcga
     1261 tgcacgctct atagcagacc tacaatctgc accgcaagac tgctactcct gccacagaca
     1321 agacgatgtt cacaacggtg agaatggtac agactgtgaa acctgtcata acccatcgaa
     1381 ttgggaagat gccgattttg accatagccg caccaacttc ccattaactg gcgggcactc
     1441 taacgtggag tgtaatcaat gccataccaa tgggacgtat aaagggcttt catctacctg
     1501 tgtcacctgc catcaagacc ctgcgtttca tgctggtgcc tttggtacag attgcgagtc
     1561 ttgccactcg atcaatgctt ggtctcctgc ccaattcaac ctgtctcacc ctgagccgcg
     1621 cgttgacgaa ggtggtagcg gtatccacca cggcggagcc tcctgccgcg aatgtcaccc
     1681 ctcatctgtc ttcgagtcta gctgtgactc ttgtcatagc ggtgggtttg aaggtggcga
     1741 aggcggagac gacgattaat agtctttgcc attaaaaaaa gagcgctagg ctaccctagc
     1801 gctctttttt tatgcggact tatcgcccta aacgatacga taggggacct gtttttccgc
     1861 tttcagttga aatctcacga ggttcaagaa gcccccaacg cccagcagcg agatagagaa
     1921 tatccagcac taagtctgta tggctgatgc cctctgccgc agcttgtaag cagaggtcac
     1981 tatagttagg ggtaagccct ggcaaggtgt tgatctcaat taggcgtgga ttgccttctt
     2041 cgtccaaccg aatgtctgtg cgagagacat caaggacatt gagaatgtta tgagcacgaa
     2101 aggcaaggtg ctttaatttt ttctcaagtt ctgggtcaat gttggctggg cagaaaaacc
     2161 cttcgttgcc ttcaggacca acatcttttg atttagcttc atttgtataa acccatggac
     2221 cttccgtatt gcctgtgtct aattccaaaa cagggaagcg gtggaagcca tctttttcat
     2281 accattcggg gtgacgagaa tatagcttgg catcggcacg gcccaagatg cccactgtga
     2341 attcgcgccc gggcaaaaag gtttcaacga gagcaggttg ctgataggtg ttgatgatgt
     2401 attctgcacg ttcgcgcaat tctgtttcgt tattgacgat ggctttcatg tctacgccca
     2461 tgcctgtccc ttcgcgggca ggtttgacga agagcgggaa tttcagctcg gggcgtaagg
     2521 gttcgtcccc aaattggaat tcttgaaagg gagccacagg cagacgacgg tcacgccaga
     2581 tacgtttggt cagtgtttta tcaagtgaga tgccattggc aagcacacgg gaaccggtgt
     2641 atggaatggc gagcatttcc agtaaagctg gcacttgtgc ttcacgggcg tcacctccaa
     2701 gaccttcggc aatattgaag cagatatctg gcttgacctt gcggaggctg taggggagtt
     2761 ctctatccgc gtggacaaag acagtagtgt gaccatccgt ttcgagcgcg gcacgaagcg
     2821 agtcaatcgt ttcgatatgg tca
//
LOCUS       DAOQNJ010000356         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25985_length_2839_cov_7.187859, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000356 DAOQNJ010000000
VERSION     DAOQNJ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25985_length_2839_cov_7.187859"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLG52_09965"
     CDS             <1..825
                     /locus_tag="PLG52_09965"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41814.1"
                     /translation="QFPDMNKYPNGGTLYHTTNGGLTWESFSTPFSGGYIQFIDANNG
                     WMLSDLGVGAGSMAVSVFQTSDAGKTWNRTYTNDPNLADASDTLPLGGIKNLILPLNA
                     KTAWIGGVVYAPGETYLFRSDDNGKTWVNINLILPQEAAGSELSVIGMVFLSPKTGLL
                     ALRVATETPKTIVYVTQDGGNTWTQSEVSFEGYGLLETPSANEMVFYVNDQFYVTKDA
                     GVTVQEIIPDVKFGDAIMDMSFANAQTGWIVASDASGNYTLYKTTDSGSTWTVLSQ"
     gene            937..1341
                     /locus_tag="PLG52_09970"
     CDS             937..1341
                     /locus_tag="PLG52_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB41815.1"
                     /translation="MNNLVRDWMSSPVVVVDPDSSVSHAMTLMRRRNIHSVVVDVSEK
                     NPSYGIVTTTDVRDKIVVAGRNPAETTVREIMSGPIVTGRAEWTLAECSRLMQERKIR
                     HLPIADEHGTLIGLISATDIFMAVEEQGWIEG"
     gene            complement(1391..1822)
                     /locus_tag="PLG52_09975"
     CDS             complement(1391..1822)
                     /locus_tag="PLG52_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNB41816.1"
                     /translation="MTLPNKLQEIVDDFASMSREEKLETLIAYAESMPELPERFHAER
                     EKMEAVPECMTPVFIAAEKDSNNGIVFHLDIPKQSPTVRGLASILTNGLNGCSLAEII
                     SVPAEFYLPMNLQEAVSQQRINGFMGVLAHMKQVAVRVGSG"
     gene            complement(1886..2725)
                     /locus_tag="PLG52_09980"
     CDS             complement(1886..2725)
                     /locus_tag="PLG52_09980"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNB41817.1"
                     /translation="MSYAHPEYLVETDWVAEHLNDPNIRIIESDEDPLLYAIGHIPGA
                     AQVDWFSTLQHPLRRDFLTKEQFEQVASKLGITNDTTVIFYGDKSNWFACYALWLFQY
                     YGHQNVKIVNGGRAKWEKEGRPLVKEVPTFTATTYKAKEADKSIRAFRDDVFKQVDSK
                     KPLVDVRSPKEYTGELLHMPNYPQEGATRGGHIPGAVSIPWAQAVNEADSTFKTAEDL
                     KALYEGKNIKSDGEIIAYCRIGERSSLTWFVLKYLLGYPTVKNYDGSWTEWGNLVDAP
                     IEK"
BASE COUNT          707 a          657 c          712 g          763 t
ORIGIN      
        1 caattccctg atatgaacaa ataccccaat ggtggtacgc tctatcacac caccaatggc
       61 ggcttaacat gggaaagctt ttctacgcca ttcagcggcg gttatattca atttattgat
      121 gcaaacaacg gatggatgct atctgacctt ggtgttggtg ctggttctat ggcggtttct
      181 gttttccaga ccagtgatgc tggcaaaact tggaatcgca cttacaccaa tgaccccaat
      241 cttgctgatg caagtgatac gcttccgttg ggtggtatta agaatctcat cttgccgctc
      301 aatgcaaaaa cagcgtggat cggtggcgtg gtctatgccc ccggtgaaac ctatcttttc
      361 cgcagtgacg ataatggcaa aacctgggtc aatatcaacc ttatccttcc gcaagaagca
      421 gcaggcagtg aactgagcgt gatcggaatg gtcttcctct cgcccaagac aggtctgctt
      481 gcgttgcggg ttgccacaga aacacccaag accattgtat acgtcaccca agatggcggt
      541 aacacgtgga cgcaatctga agtttcgttt gaaggttatg gattattgga aacgccgtca
      601 gcgaatgaaa tggtctttta tgtcaatgac cagttctacg tcacgaaaga tgcgggtgtt
      661 actgtccaag aaattatccc cgatgtgaag ttcggcgacg ccatcatgga tatgagtttt
      721 gcgaatgctc aaactggttg gatcgttgct tctgatgctt ctggaaacta tactttgtat
      781 aagacaacgg atagcggctc aacatggacg gttctaagtc agtaaacgct tcttcccccg
      841 ccgctgcttg atactattgc tcgacccttt ggttacgctg tgagtaacct gatagttaca
      901 cccatactgt aaaatatcta taaaggagag agaattatga ataacttagt tcgtgattgg
      961 atgtccagcc cggttgttgt tgtagaccct gattcgagcg tttcacatgc catgacgctc
     1021 atgcgtcgtc gcaatattca tagcgtggtt gtggatgtta gcgagaagaa cccttcatat
     1081 ggcattgtaa caacaacaga tgtgcgcgat aagatcgtgg ttgccgggcg taacccagca
     1141 gagaccactg tgcgcgagat tatgtctggt cccattgtta ccgggcgtgc cgaatggacg
     1201 ctggcagaat gctctcgact gatgcaagaa agaaaaattc gccacttgcc aattgcagat
     1261 gaacatggca ccttgatcgg cttgatctct gccaccgata tttttatggc agtggaagaa
     1321 caaggttgga tcgaaggcta gaaaataaaa acagtcccgt aaagggactg ttttttgtta
     1381 cttgtaaaat ttaccctgac ccaacccgca cagcaacttg tttcatgtgt gccagtacac
     1441 ccatgaaacc attaatgcgc tgttgggata cggcttcttg tagattcata ggcaggtaaa
     1501 actcggcagg cacagaaata atctctgcaa gcgagcagcc atttaagcca ttggtcagaa
     1561 tcgatgccag tccgcgcacg gtgggagatt gtttcggaat atccaagtgg aagacgatgc
     1621 cgttgttgct gtctttctcg gcggcaatga aaacgggcgt catgcattct ggcaccgctt
     1681 ccattttttc gcgttctgca tggaagcgct ctggcaactc tggcatggat tcggcgtatg
     1741 cgatcagcgt ctccagcttt tcttcgcggc tcatgctggc aaagtcatct acaatttctt
     1801 gaagtttatt gggtagggtc ataatgttcc gggtaagggc gaggttatac cactctgtga
     1861 tataaacctc gcctctacaa tactattatt tttcaattgg tgcatctacg aggttgcccc
     1921 attcagtcca ggaaccgtcg tagttcttga ccgtgggata acccaacaaa tacttcaata
     1981 cgaaccaagt caacgaagag cgttccccaa tacgacaata ggcgatgatt tcaccatcgg
     2041 atttgatgtt tttgccttcg taaagcgcct tcaagtcttc agcggtctta aaggtggaat
     2101 cggcttcgtt gacagcctgt gcccacggaa tgctgaccgc tccggggatg tgtccgccgc
     2161 gggtggcgcc ttcttgtggg tagttgggca tgtgcaataa ttcgcccgta tattccttcg
     2221 gcgagcggac gtctaccaag ggtttctttg aatcaacctg cttgaagaca tcgtcgcgga
     2281 aggcgcgaat ggacttatcg gcttctttgg ctttgtaggt agttgcagtg aatgtgggaa
     2341 cttccttcac gagtgggcga ccttcttttt cccatttcgc acgtccgccg tttacgatct
     2401 tcacgttctg gtgaccataa tattggaaga gccagagcgc atagcaggca aaccaattcg
     2461 acttatcgcc ataaaagatg acagttgtat cattggtgat gccaagcttg gatgcaacct
     2521 gttcaaattg ttctttggtg agaaagtcgc ggcgcagtgg gtgttgaagc gtgctgaacc
     2581 agtctacttg ggcggcgccg ggaatgtgac ctatggcata cagcaaggga tcttcgtccg
     2641 attcgataat acgaatgttg gggtcattta gatgttcagc gacccagtct gtttctacaa
     2701 ggtattcggg gtgagcgtac gacatgataa agtttccttt tctaagcaag aagtatatgc
     2761 aaaataaaaa gcagccaacc gaacaaaaag gacagcgaac catcctgcgc ggacatcatt
     2821 tcacgcggga gccgttcca
//
LOCUS       DAOQNJ010000357         2838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_25998_length_2838_cov_9.722961, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000357 DAOQNJ010000000
VERSION     DAOQNJ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25998_length_2838_cov_9.722961"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="PLG52_09985"
     CDS             <1..1473
                     /locus_tag="PLG52_09985"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41818.1"
                     /translation="QQIYLQRDKIFVKEDLSGDVAEMLDNEVTKRVEVGLADEEGPWR
                     LLAWLDQVQPAFDSKNGLFPSYGFKLLLDQINPSDVRNSTLDLVSKSIEAEYAHALRA
                     IETMIEKTGETLETQIAEREDSLDAFFQTLGDREDAPKAQKLVEEINSLTRLAIRPSN
                     DLLKKLADDPEEAKEDIQEMIASQLTIISATRVIGAIQNRIGEQVQWQNPLPSDWDEL
                     SDILLTTSRDAFEKKRERLNAQVVRDIDVLLQREDVSTESGRLRLLITLSQGTRTAFD
                     QKTHKQVKQVFTRFAYAFYAAQLLEGREAEEIADDVMNHLEAAEESLRETWGQSEFMR
                     LSQNATKLADFGPAARIAFGEEKLNNAPSSLSADEAAQLSSALGKYVLNEVHRQLLLG
                     AFSELWVEYLTKVEALRVSIGLEAYAQRDPLVQYKRSASEMFQQLLEDVRGLVISRAF
                     AARPRRVEITPIETTETAATPNETSVQIGGDSKKKKRRRS"
     gene            1491..2531
                     /gene="add"
                     /locus_tag="PLG52_09990"
     CDS             1491..2531
                     /gene="add"
                     /locus_tag="PLG52_09990"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNB41819.1"
                     /translation="MADDTTTPFNKYVALPKVELHRHLEGSLRLSTMLDIARKHGVTV
                     PVSMLNLSGLVQVQDQDPMTFSNFLEKFKTLRLFYRSPDVIHRVTREAVEDAARDNVR
                     YMELRFTPVALSRAEGFPLHDVMDWVITSAQEAAREYKIKVGLIASVNRHESPELAEQ
                     VSWLAVEHMKNGLFGLDLAGNEAEFKSHPFRDIFKEAKQSGLRITIHAGEWGPADNVR
                     DAIEHLGAERIGHGVRVLEDEAVATLAMERGAVFEVCVTSNFQSGVINEVEKHPLPRM
                     LEKGLKVTVNTDDPSVSRITLSNEFQSIHEKLNISMDVLKQSVITSAEASFLPDAEKA
                     DLVKKLKEELKR"
BASE COUNT          731 a          667 c          768 g          672 t
ORIGIN      
        1 cagcaaatct acttacagcg cgacaagatc ttcgtcaaag aagatttgag cggcgatgtg
       61 gcagaaatgc tcgataacga agtgaccaaa cgcgtagaag ttggcttggc agacgaagaa
      121 ggtccgtggc gtttattggc gtggttagac caagttcaac ctgctttcga ttcaaagaac
      181 ggcttgttcc cctcttatgg atttaagcta ttgcttgacc aaatcaatcc ttccgacgtc
      241 cgcaattcga ccttggactt ggtctctaaa tccatcgaag ctgaatacgc tcatgccttg
      301 cgtgccattg agacgatgat cgaaaagaca ggcgagactc tcgagacgca aattgcagaa
      361 cgagaagaca gcctcgatgc cttcttccaa accttaggtg accgtgaaga tgcgcccaaa
      421 gcccagaagt tggtggaaga gatcaacagc ctgacccgct tggcaattcg cccgagcaat
      481 gacttgctca agaaattggc agatgaccct gaagaggcaa aagaagatat tcaggaaatg
      541 attgccagcc agttgaccat tatcagtgcg actcgcgtga ttggtgctat tcaaaaccgc
      601 atcggtgaac aagttcagtg gcagaacccg ctcccttcgg attgggatga actttcggat
      661 attttgttga ctacttcacg cgacgctttt gaaaagaagc gagaacgctt gaacgctcaa
      721 gtggtgcgtg atattgacgt gttgttacag cgtgaagatg tctcgaccga gtcggggcga
      781 ttgcgtttgt tgatcaccct ctcacaaggc acccgcactg cctttgacca gaaaacccat
      841 aaacaggtta agcaggtctt tacccgtttt gcatatgcct tctatgctgc tcaattgctg
      901 gaaggtcgcg aagccgaaga gattgccgat gatgtgatga atcacctcga agcggcagaa
      961 gaatccctgc gcgagacgtg gggacagagc gagttcatgc gcttgagcca gaacgctacc
     1021 aaactcgcag actttggtcc ggcggcgcga atcgctttcg gcgaggaaaa gctgaataac
     1081 gctccatcca gcctgagcgc ggacgaggcg gcgcagctat cctctgcatt gggcaagtat
     1141 gtattgaatg aagtccatcg ccagttgtta ttgggtgcgt tcagtgaact gtgggtggag
     1201 tacctcacca aggtcgaagc cttaagagtt tcgataggtc ttgaggctta cgcccaacgt
     1261 gacccgctgg tacaatacaa acgcagtgca tcagagatgt tccagcaatt gctcgaagat
     1321 gtacgcggac ttgtgatcag ccgtgcgttt gcggcacgac cgcgacgggt ggagatcaca
     1381 ccgattgaaa ccacagaaac agctgcgaca ccgaacgaga cgtcggtgca aatcggcggt
     1441 gatagcaaaa agaagaagcg ccgccgttcg taactcgtaa gaggaagcgt ttggcagacg
     1501 atactacaac cccttttaat aaatatgttg cccttccaaa ggtggaactt catcgccatt
     1561 tggaagggtc tttgcgtctc tccaccatgt tggatattgc gcgcaaacac ggggtgacgg
     1621 ttccggtttc gatgttgaac ttaagcgggc tggtgcaagt gcaagaccaa gacccgatga
     1681 ccttctcgaa cttccttgaa aaatttaaga ccttgcgttt gttctatcgc tcacccgatg
     1741 tgattcatcg cgtcactcgt gaagcggtgg aagatgcggc tcgtgacaac gtccgctaca
     1801 tggagttgcg ttttacgccc gtggctttga gtcgcgccga gggcttcccg ctgcatgatg
     1861 tgatggattg ggtcattacc agtgcgcagg aagctgccag agaatacaag atcaaagttg
     1921 ggttgatcgc ttcggtgaac cgccatgaaa gcccggaact tgcggagcag gtttcgtggt
     1981 tggcggtgga acatatgaag aacggtttgt tcggcttgga tttggctggc aatgaagcgg
     2041 agtttaagtc tcatcccttc cgtgatattt tcaaggaagc caaacaatcg ggtttgcgta
     2101 tcaccatcca tgccggggag tggggacccg cggataacgt ccgcgatgcg attgagcatt
     2161 taggcgcgga gcggatcggt cacggcgtgc gtgtgttgga agatgaagct gtggcaactt
     2221 tggcgatgga acgaggcgcg gtctttgagg tctgcgtgac tagtaacttc caatcgggcg
     2281 tgatcaatga agtggagaaa catccgttgc cgcgcatgct cgaaaaggga ctcaaggtca
     2341 cggtcaatac cgacgaccca agtgtttcgc gcatcactct ttcgaacgaa ttccagtcca
     2401 ttcacgagaa attgaacatt tcgatggacg ttttgaaaca gtctgtgatc acgtcggcag
     2461 aagcgtcctt tttgcccgat gctgaaaaag cggatttggt caaaaaactc aaggaagaat
     2521 tgaagcgtta agacagtccg gtaaataggg cgtgatttgg aaaagacact tccacgcacg
     2581 cccttgaatt agtataattt agctctgaat ttctctcgtt aaccccacca cattaaaaaa
     2641 ggatatcaaa ggcgctcctt tgtgttcttc ctgattgtgg tgtgagtgta atgtcgaact
     2701 tatttcgata ttacgaaaaa cctcatacca aaggagtatc ctatgcaaga cctatttaag
     2761 tcccgtgacg tattgaccgt gggcaagaag aaatacgtct atttccggct cgacgcgctc
     2821 gaaaaagccg ggctgacc
//
LOCUS       DAOQNJ010000358         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26140_length_2829_cov_6.659337, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000358 DAOQNJ010000000
VERSION     DAOQNJ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26140_length_2829_cov_6.659337"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLG52_09995"
     CDS             <1..796
                     /locus_tag="PLG52_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41820.1"
                     /translation="ILDPLKGQTDLERGVIRVLHPNSFIDDPTRIFRAIRYEARYGFS
                     MDEETSGLVNTEALKVLHSLSGERIRHELDLILDEDESSKMMLRIVNLGVLSAIHHKM
                     PEFNQDYADFLEMDVRLDVQADRRTMGYILWFMDLSEEDVILLSNRLDFTNELTLAVW
                     AGAQLRRSLIHLVGARPSEWTYALEKLPLLSIYVVYLVSRENDLLSYISLWRHVKPQT
                     TGNDLKAKGLLPGPRFSEILLRLRSAWLDGEVNNESQEKELLAKLL"
     gene            793..1557
                     /locus_tag="PLG52_10000"
     CDS             793..1557
                     /locus_tag="PLG52_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNB41821.1"
                     /translation="MNLQPYLEFATSLAYHAGKITLRYFNTGIVPDRKENNDPVTIAD
                     REAEAFVRSQVEKHYPGHAIVGEEYGEQGTGHSFRWIVDPIDGTISFVQGVPLYGVLI
                     ALEIDGVVQVGAAYFPPLDEMLTAGSGLGCWWNGRRARVSGVSKLSEACVVTSDFQHL
                     EKKQAGITKRFEESGALLRTWGDAYGYLLVATGRAEVALDPRMDVYDCGPYPVIMKEA
                     GGYFGSWIGEEGHDHGEGLACNAALKPDVMKLMRGK"
     gene            1603..2184
                     /locus_tag="PLG52_10005"
     CDS             1603..2184
                     /locus_tag="PLG52_10005"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNB41822.1"
                     /translation="MTEDKSSRLPYRLREPFLSNTEAALFRLLTDLMGDRYMICPKVA
                     LTDLFMIVRPNENVHFFNKIFRKHVDFLLCDPKTFTPTFGVEIVKSIAKDGMREADKF
                     LAELFTDAGIPLVHIPTNDKYEPADLIALFQLAVLKVGKNIPVEVAMDSVPSCPVCGK
                     MMVLRMHRNGNSAGKKYYGCIDNPTCAGTVAID"
     gene            complement(2201..>2829)
                     /locus_tag="PLG52_10010"
     CDS             complement(2201..>2829)
                     /locus_tag="PLG52_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41823.1"
                     /translation="AAVKAGVKRIVHISIANPSADSHLPYYWGKAANEKAVTESGMSY
                     AILRPTVLVGGGEDILINNIAFLLKRFPIFFVPGDGQYGIQPVHVEDLAELAVQGVYS
                     KENYVIDAVGPDSYTFKGLIELVRDTVGAKSLLISMPPRLALLAAQFLSLFVNDVILT
                     PEEVDGLMGNLLVSKEPARGKTLFKDWLKENRKIVGAIYASELKKHYV"
BASE COUNT          698 a          609 c          753 g          769 t
ORIGIN      
        1 aatcctcgac ccgctgaaag ggcaaacaga cctcgagcgg ggagtgatcc gcgttttaca
       61 tccgaattcg tttattgatg acccaacccg catctttcgc gccattcgct acgaagcacg
      121 ctatggattt tcgatggatg aggaaacctc tggcttagtc aatacggagg cgttgaaggt
      181 tttgcattcg cttagcggag aacgcatccg ccatgaattg gatttgattc tggatgaaga
      241 tgaatcctca aaaatgatgc tgcgcatagt caacttgggc gtgttgagtg ctattcacca
      301 caagatgcca gagttcaatc aagattatgc tgattttctt gagatggatg ttcgtttaga
      361 tgtacaagcc gaccggcgca cgatgggtta catcttatgg tttatggatt tatcggaaga
      421 agatgtgatc ttgctttcga accgcttgga ttttacgaat gaactgacgc tggcggtttg
      481 ggcgggggcg caattgagac gtagcttgat tcatcttgtt ggcgcgcgcc ccagtgaatg
      541 gacctatgct ttagaaaagc tgccgctact ttctatttat gtggtgtatc tggtctcacg
      601 tgagaatgat ttgttgagtt atatctcact ctggcggcat gtcaaaccac agactacggg
      661 gaatgatctc aaggcaaaag gcttgttgcc gggtccgcgt tttagcgaga tcttattacg
      721 tttgcgctct gcctggttgg atggggaagt gaacaatgaa tctcaagaaa aggaattgct
      781 tgccaaactt ctatgaacct tcagccctat ctcgaattcg ccacttccct tgcgtatcac
      841 gcggggaaga ttactttaag gtacttcaac acaggcattg tgccagaccg taaagaaaac
      901 aatgaccctg tgaccattgc tgaccgcgaa gccgaagctt tcgtgcgctc gcaggtggaa
      961 aagcattatc cgggtcatgc cattgtgggg gaggaatatg gcgagcaggg aacgggtcac
     1021 tcattccgct ggatcgtaga ccccattgat gggacgatct cgtttgtgca gggtgttcct
     1081 ttatatgggg tattgatcgc tctggagatt gatggtgtgg tgcaggtagg agctgcttac
     1141 ttcccgccgc tggatgagat gttgacagcg ggcagcgggc tgggctgttg gtggaatgga
     1201 cgccgcgcac gtgtttcagg cgtgagtaaa ctttcagaag cctgtgtggt cacttcagat
     1261 tttcagcacc ttgaaaagaa gcaggcgggc attacaaaac gttttgaaga aagcggcgct
     1321 ctgctgcgga cgtggggtga tgcttatgga tatttattgg tcgctactgg gcgtgcagaa
     1381 gtggcactag acccacgcat ggatgtgtat gattgcggtc cgtacccggt gattatgaaa
     1441 gaagcaggtg gttatttcgg ttcgtggatt ggcgaagaag gtcatgacca cggcgaaggg
     1501 ttggcttgta acgccgcact caagccagat gttatgaaat tgatgcgcgg aaagtaggtc
     1561 aaatttctgt acgtagattc aaacctaacg tataatcgaa ttatgaccga ggataaaagc
     1621 agccgacttc cttaccgtct tcgagagccc ttcctaagca atacggaagc ggctcttttt
     1681 cgtttgttga cggatctgat gggtgatcgt tatatgattt gccctaaggt tgctctgaca
     1741 gacttgttca tgatcgtacg cccgaacgaa aacgttcact tttttaataa gattttccgc
     1801 aagcatgttg attttctatt gtgcgacccg aagaccttca ctcccacctt tggggtggag
     1861 atcgtcaagt cgattgcaaa agatggtatg cgtgaagccg ataagttttt agcggaactt
     1921 tttactgatg ctggaattcc gctggttcac attcctacga acgataaata tgaaccagca
     1981 gatttgattg cactgttcca actggcagta ttgaaagtgg ggaaaaatat tccagtagag
     2041 gttgcaatgg attcggtgcc aagttgcccg gtttgtggca agatgatggt tttgcgtatg
     2101 catcgtaatg gcaacagcgc cggaaaaaaa tattacggat gcatcgataa cccgacctgt
     2161 gcaggtaccg tagcgatcga ctagattgct tttgggggtg ttagacgtaa tgctttttta
     2221 attcagatgc atagattgca ccaacaatct tacgattttc ctttagccaa tctttgaata
     2281 aggtttttcc acgggcaggc tctttggaga ccagcagatt tcccattaat ccatccactt
     2341 cttcaggggt gagaatcacg tcattgacga aaagactcaa gaattgagcg gcgagtagtg
     2401 ccagacgtgg cggcatggag atcaataaac tctttgcgcc aaccgtatct ctgactaatt
     2461 cgatcagtcc tttgaatgta tacgaatctg gacccacagc gtcaatcaca taattttctt
     2521 tgctataaac cccttgaacc gccaactctg cgaggtcttc gacgtgcact ggttgaatac
     2581 catactgccc atcgccgggg acaaagaaaa tggggaagcg cttcaagaga aacgcaatat
     2641 tgttgatgag gatatcttcg cctccgccga cgagcaccgt tgggcgcaga atggcatagg
     2701 acattccaga ctcggtcaca gctttttcgt tggcagcttt tccccaataa taaggcagat
     2761 gcgaatctgc agatgggttt gcgatgctga tatgaacgat gcgtttgacg ccagctttga
     2821 ccgcggctc
//
LOCUS       DAOQNJ010000359         2827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26177_length_2827_cov_11.170635, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000359 DAOQNJ010000000
VERSION     DAOQNJ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26177_length_2827_cov_11.170635"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLG52_10015"
     CDS             <1..470
                     /locus_tag="PLG52_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41824.1"
                     /translation="ELWARDVRFILGTAPNHPPMLSPAELIKGLAVSVETRLQLSLIP
                     LFLRHPEFSTCAESVAKNLPLERQLILKCFYCAAVWLERKHLSQNNLPDLFSKDIGLS
                     PTSDPDNNLLSLANRQKELSGSRINWAATYQHAADIWLKDLELRKMKNGKRNS"
     gene            451..963
                     /locus_tag="PLG52_10020"
     CDS             451..963
                     /locus_tag="PLG52_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41825.1"
                     /translation="MEKETLESTLKEFGKRVPPGSQLHLLGGGALTLLGSPRPSLDID
                     FVGDDVHPNELHRTILTISKEMDLFVDAVPIDRFVPLPEGHENRAIHIGQFENLEIFV
                     IDPYSIALSKVDRGAFTDYDDILFLIKSGYITIVELEKIVQKAITKAGKYDLHPEILI
                     HFEELKSRLK"
     gene            1005..1658
                     /locus_tag="PLG52_10025"
     CDS             1005..1658
                     /locus_tag="PLG52_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41826.1"
                     /translation="MSEFFQRNLPHYHLPNATYFITFRLAGSLPAEVWKNLKEKYEQE
                     KHLLAGKFKGNKLREERYKAQKRHFARFDALLDHSKDSPRWLAEPQFAEAVQQELHTL
                     CPEHYNLHSFCIMSNHCHLLIDQQNTPKPIAPIDGKHYTSLSHAMRLLKGRTGYACAK
                     LLGRKGAFWQHESYDHVVRDEKEFFRILGYIANNPVKAGLVENWQDWDYTYINPTLL"
     gene            1695..>2827
                     /locus_tag="PLG52_10030"
     CDS             1695..>2827
                     /locus_tag="PLG52_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB41827.1"
                     /translation="MNITLKINGIEQIIDAPASTTLLAALRGLGFHGVKFGDEQGLTG
                     ADTILLDGKPVNAGSMLAAQADGHAVATIEALGEHPDLGWRKTDGLHPLQKAFVESGA
                     IQCGYCTPAQILAAKSLLEKNPNPSEADVREAIAGVLCRCTGYLKPVQAVMKAAAEMR
                     GEEIGDGEAINWDLGNWGNRPEPTNLPSDQFTNVMTKPKVVVTPETQKWQTVGKPEKK
                     VDAIKLVQGKPAFTADMDMRGMLYAKVLRSPHAHARIKNIDTSKALALKGVVSVLTWK
                     DLPRVVYSTAGQSDPIPGPLDSFSLDNKVRFVGDRVAFVAAETPEIAEKALALIDVEY
                     EVLPAILDSRESMNPDAIKIHDEPEYINFADSNPDKNLAAHIR"
BASE COUNT          803 a          673 c          665 g          686 t
ORIGIN      
        1 cagaactttg ggcaagggat gtgcggttca ttcttggaac tgcgccaaat cacccgccca
       61 tgctttcgcc tgcagaattg ataaaagggc ttgcagtgag cgttgaaaca cgtttacagt
      121 tgagtctgat tccattgttc cttcgccatc ccgaattctc tacttgcgcc gaatctgttg
      181 caaaaaatct acctttggaa aggcagttga tattaaagtg tttttattgc gcagcagtgt
      241 ggctcgaacg caaacatctt tcacaaaaca acctgcctga cctgttttca aaagatatag
      301 gattatcgcc cacatctgac ccagataaca atctgctttc gcttgcgaac cgacaaaaag
      361 agttaagtgg ttctcgaatt aattgggcag ccacctacca acatgcagcg gatatttggt
      421 tgaaagacct tgagttgcga aagatgaaaa atggaaaaag aaactcttga aagtacttta
      481 aaagagttcg ggaagcgcgt tccacctggt tcgcagttgc accttcttgg tggcggagca
      541 ttaaccctgc tgggaagtcc gcgaccttct cttgacattg attttgtagg cgatgatgtc
      601 catccgaatg agttgcatcg tacaatcctg accatttcca aagaaatgga tttatttgtt
      661 gatgcagttc ccattgaccg attcgtccca ttaccagaag gtcacgaaaa ccgcgccatt
      721 catataggtc aatttgaaaa cttggaaata tttgttattg atccatacag cattgcgttg
      781 agcaaagtag accgaggcgc atttaccgat tatgacgata tcctcttttt gatcaagtct
      841 gggtacatca ccatagtaga attggaaaaa atcgtacaaa aagccattac gaaagcaggc
      901 aaatatgacc tgcatcctga aattctgatc cattttgaag aattgaaatc ccgcttaaaa
      961 taatgtaagg caagtctata gacttgcctt acaaagctaa gctcatgagc gaattctttc
     1021 agcgcaacct ccctcactac catctcccaa atgcaaccta ttttataaca tttcggctgg
     1081 caggcagttt acctgcggag gtgtggaaaa atttgaaaga aaaatacgag caggaaaaac
     1141 acctgctggc tggtaaattc aaaggaaaca aattgcgtga agaacgctat aaagcgcaaa
     1201 aacgtcactt cgcccgcttc gacgcgttgc ttgaccattc caaagatagc ccgcgctggc
     1261 tagcagaacc tcaatttgcg gaggcggttc aacaagaact tcatacccta tgtcccgaac
     1321 attataattt acactctttt tgcattatgt ccaatcattg tcatttgctt attgaccaac
     1381 aaaatacccc aaaaccgata gcacccattg acggcaagca ttacacatct ctaagccacg
     1441 ccatgcgcct ccttaaaggg cggacaggat acgcctgtgc aaaactatta gggcgcaaag
     1501 gtgcattctg gcaacatgaa agttacgatc atgtcgttcg cgatgaaaaa gagtttttcc
     1561 gaatcctggg atacatcgca aacaaccccg tcaaggcagg tttggtcgag aattggcaag
     1621 actgggacta tacttacatc aatcccactt tgttgtagaa caagtctata gacttgttct
     1681 acaaggagta atccatgaac atcacactca agatcaacgg aattgaacaa atcatcgatg
     1741 cacctgcatc caccaccctg ctagccgccc tgcgcggact tggttttcac ggcgtgaagt
     1801 ttggcgatga gcaaggactc accggcgcgg atacaattct gctggatggc aaacccgtca
     1861 atgcggggtc gatgctagcg gcgcaggcgg atggtcatgc cgtggcaacg atcgaagcct
     1921 tgggcgagca cccagacctc ggctggagaa agaccgatgg tcttcatccg ctgcaaaagg
     1981 cttttgttga atctggcgcg atccaatgtg ggtattgcac cccggcgcag atccttgccg
     2041 caaaaagttt gcttgagaaa aatccgaacc caagtgaagc ggatgtccgc gaggcgattg
     2101 cgggtgtgtt gtgccgctgt acgggttatc tcaaaccagt gcaagccgtg atgaaagccg
     2161 ccgccgaaat gcgcggtgaa gaaattgggg atggcgaagc gatcaattgg gatttgggga
     2221 attggggcaa caggcccgag ccgaccaact tgccaagtga ccaatttaca aatgtaatga
     2281 ccaagccaaa agtggtggtg acgcctgaga cacagaagtg gcaaaccgtg ggcaagcctg
     2341 agaagaaagt ggatgccatc aaattggtgc aaggcaagcc cgccttcacc gcagacatgg
     2401 atatgcgcgg catgttgtac gcaaaggtct tgcgttctcc gcacgctcat gcgcgaatca
     2461 aaaacattga caccagtaaa gctttggcat tgaaaggtgt tgtttctgtt ttgacatgga
     2521 aagacctgcc gcgtgtagtc tattccacag ccggacaatc agacccgata cctggtccgt
     2581 tggattcgtt ctcattagat aacaaagtgc gttttgtcgg tgatcgcgta gcgtttgttg
     2641 ccgcggagac tcctgaaatc gcagaaaagg cgctcgctct cattgacgtg gaatatgaag
     2701 ttctgccagc tattctagac tcacgcgagt ccatgaatcc agatgcgatc aaaatccatg
     2761 acgagcctga atatataaac tttgcagact cgaaccctga taaaaatctc gcggcgcaca
     2821 tccgcat
//
LOCUS       DAOQNJ010000360         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26230_length_2824_cov_9.068978, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000360 DAOQNJ010000000
VERSION     DAOQNJ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26230_length_2824_cov_9.068978"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..959
                     /gene="recO"
                     /locus_tag="PLG52_10035"
     CDS             201..959
                     /gene="recO"
                     /locus_tag="PLG52_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNB41828.1"
                     /translation="MTDFRSFRASAVVLRHADWGEADRILTLYTRDQGIVRALAKGAR
                     KVTSRKAGHLQPFTHVTVQLAKGRDLLIITQVETINAYLPLHDDLIKTGYAAYIIELL
                     LRFSYEDEGANPAIFRLVTETLDRLEKETDAWLPVRYYEMRLLDAVGFRPQLFQCANC
                     SREILPEDQYFSFTAGGVICPRCGEGIPNLAHISLEMLKYLRHFQRSSYKEASKARPS
                     LEVQKEAEVLMQGYFTYLLERELNTPGFLKKVKT"
     gene            complement(970..1740)
                     /locus_tag="PLG52_10040"
     CDS             complement(970..1740)
                     /locus_tag="PLG52_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNB41829.1"
                     /translation="MGTADLHVHSIYSRDATTTVRAILKQASSVGLDIIAVTDHDEIR
                     GSLEAQQLAPQYGIEVISGAEVSTKDGHLVALFIKDLPPAGMSLTDTLLHIGDQGGLA
                     IIPHPFNNLPNSLTLEAVFTAMENPTAKASLKGMETHNFGTQNFDKMARKLSVFLPFS
                     KISSSDAHIYWAIGAGRTEFPGKTTQDLRDAIENNKTVPLLYEGESSAKALLSWARRI
                     TMRRFGYASDTSSATEDINTRQMSDSLIRRIQKKESSS"
     gene            complement(1770..>2824)
                     /locus_tag="PLG52_10045"
     CDS             complement(1770..>2824)
                     /locus_tag="PLG52_10045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB41830.1"
                     /translation="KEHYPRLKLCAGGLVIDAERILEHLGLDVTEIPHVDSNSIRFDF
                     AGSGINIRVPKRHALRVIRRDEFDHWLAKQVESKGIEIRQGVTVRKISPDANGVTVET
                     DQETLRATLVVGADGSNGITRRSIFPNEPIYTARVLEVLTPVSPVSSNSTTPRNQTAL
                     FDFFPVPDNIAGYVWDFPTQIKGMEMRCWGIYDTNLLASQKRPALKDPLAKEMQRHGF
                     DLNEYEIKGHPIRWYSPGNKASVSRVLLVGDAVGADVIFGEGISMALGYGNLAAKEIA
                     DSFQRGDFSFRRYKRRLERSGLGQTLFARWLIAQIIYNLKWRWFQILLWRIVKPIVLF
                     VAWVFVLNWSQRDLRY"
BASE COUNT          706 a          662 c          688 g          768 t
ORIGIN      
        1 gcggttctcc ttgcggcgga tgcaggcaag taatggcaga gttcggcttg gatgctattg
       61 tattgttcgg caacgggcaa ggtgagttga cacatgaaac tacagttcat gaccttttac
      121 ctgaagcctt taccccaaaa catttagtga ctgaatagaa acttgtggag aatggacaaa
      181 accattctcc atgttctttt atgaccgact ttcgttcgtt tcgtgcttct gcagttgtgc
      241 ttcgccatgc ggactggggc gaagctgacc gtattctcac gctttacact cgtgaccaag
      301 ggatagtgcg tgcgttagca aaaggtgcca gaaaagtcac atcacggaaa gccgggcatt
      361 tacaaccttt tacccatgtc acggttcagc ttgctaaagg gcgagactta ctgattatta
      421 cccaagttga aacgattaat gcttatttac ccttgcatga tgacttgata aaaactggtt
      481 acgccgcata cattattgaa ttgcttttac gcttttcata tgaagatgaa ggcgcgaacc
      541 cggcgatttt ccggcttgtg actgaaactt tggatcgcct tgaaaaagag acagatgctt
      601 ggttacccgt ccgctattat gagatgaggt tattggatgc tgtagggttt cgcccgcaat
      661 tattccaatg cgctaattgt tcgcgcgaaa ttttgccaga agatcaatat ttctcgttta
      721 cggcgggagg ggtgatctgc ccccgttgtg gggaggggat tccgaacctg gcacatattt
      781 cattggaaat gctcaaatat ttgcggcatt tccaacgttc gagctacaaa gaagcctcaa
      841 aagcaagacc cagtcttgag gtccagaaag aggcggaagt tcttatgcaa ggctatttca
      901 cttatttgtt ggaacgagaa ttaaataccc ccggcttcct aaagaaggtc aaaacataaa
      961 agagagcagt tacgaactgc tctctttttt ttgaattcta cggataagag aatctgacat
     1021 ttgccttgta tttatatctt ctgtcgcaga actcgtatca gaagcatacc caaacctgcg
     1081 catggtaatg cggcgcgccc aactcaaaag cgccttggca gatgattcgc cttcatagag
     1141 cagtggcaca gttttgttat tctctattgc atcacgcaga tcttgcgtgg ttttaccagg
     1201 gaactctgtg cgcccggcgc caatagccca atagatatgg gcatcgcttg aagatatttt
     1261 tgagaatgga aggaacacag aaagtttacg cgccattttg tcaaagttct gtgtgccaaa
     1321 gttatgggtt tccattcctt taagcgatgc ctttgctgtg gggttttcca tggcagtgaa
     1381 tacggcttcc aacgtaaggc tgtttggaag attattgaat gggtgaggga tgatggcaag
     1441 cccaccctga tctccaatat gcaacaaagt atcggttaga gacatgcctg ccggcggaag
     1501 gtccttgatg aacaatgcca ccaagtgccc gtcctttgtg ctaacttcag ccccagaaat
     1561 aacttcgatt ccatattgtg gtgccagttg ttgtgcttcg agagagcccc ttatttcatc
     1621 gtgatcggtc actgcaataa tgtcgagccc gacacttgaa gcttgcttga ggatggctcg
     1681 cacagtggtg gttgcgtctc gggagtagat ggagtgaacg tgaaggtctg cggtgcccat
     1741 gtgctgttac tttcttttct tttatatgtt caatatcgga ggtctctttg actccaattt
     1801 agtacaaaaa cccatgccac aaaaaggacg attggtttca cgatgcgcca aagcaggatt
     1861 tggaaccagc gccatttgag gttataaata atttgcgcga tgagccaacg tgcaaataac
     1921 gtttgaccca acccgcttct ttccagccga cgcttgtacc tgcgaaacga aaagtcacca
     1981 cgctggaacg aatctgcgat ctctttggcg gcgaggttcc cataccctaa tgccatacta
     2041 atgccttcgc caaagatcac atcggcacct actgcatctc ctaccagtaa aacccttgaa
     2101 accgaagcct tattccccgg gctgtaccaa cgaatggggt gccccttgat ttcatattca
     2161 ttcagatcaa aaccatgtcg ctgcatttct tttgcaagcg ggtctttaag tgccgggcgt
     2221 ttctggcttg caagcagatt tgtatcgtag atgccccagc aacgcatttc cataccttta
     2281 atttgggttg gaaaatccca aacgtacccc gcaatattat cagggactgg aaaaaagtca
     2341 aacaaagctg tctggtttct gggtgttgtg gagttacttg aaaccggcga aaccggcgtt
     2401 agtacttcca gtacgcgggc tgtataaatc ggctcgtttg ggaaaatgct gcggcgtgta
     2461 atgccattcg agccatctgc gccaacgacg agggtagcac gtagggtctc ttgatcggtc
     2521 tcaacggtga cgccattggc gtctggagag attttcctaa ccgtcacacc ttggcgaatc
     2581 tcgatgccct tgctttcaac ttgctttgcc aaccagtgat cgaactcgtc gcggcgaatc
     2641 acgcgcaggg cgtggcgttt cggtacacgg atgttaatac cgctgcctgc gaaatcaaag
     2701 cggatcgagt ttgaatccac atgcgggatt tcggtaacgt ccaagccgag atgctcgaga
     2761 atccgttcag catcgatgac caagccgcca gcacaaagct tcaggcgtgg ataatgttcc
     2821 tttt
//
LOCUS       DAOQNJ010000361         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26295_length_2820_cov_10.428210, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000361 DAOQNJ010000000
VERSION     DAOQNJ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26295_length_2820_cov_10.428210"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /gene="folK"
                     /locus_tag="PLG52_10050"
     CDS             complement(<1..292)
                     /gene="folK"
                     /locus_tag="PLG52_10050"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNB41831.1"
                     /translation="MSDLHYVYLNLGSNLEPEKNIPLAITLLKKVGEVSAVSSVWETE
                     SVGYAGPNFLNVCVLLLTSLDAEHIKYEINRPIEAQMGRVRGENTYAPRPIDI"
     gene            complement(285..641)
                     /gene="folB"
                     /locus_tag="PLG52_10055"
     CDS             complement(285..641)
                     /gene="folB"
                     /locus_tag="PLG52_10055"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNB41832.1"
                     /translation="MDKVFIKDLLVRGIIGIREWEREKEQDILINVTVYTDTLRAAET
                     DDIEDCVDYSALAKKIQSHAETAKRLTVEALANDLAKICLETPLVRKVVVRVEKPGAV
                     RFAKSVGVEVERKRDE"
     gene            complement(653..1378)
                     /locus_tag="PLG52_10060"
     CDS             complement(653..1378)
                     /locus_tag="PLG52_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB41833.1"
                     /translation="MYDLTGKTILITGAARRLGRLFALACGRAGADLILHHGHSPQEA
                     ESLRDELASLGRRAWVLAADFSHPESASQLIERANELSPLYGLVNSASIFEPLSLQET
                     SLDDWNYHLNINLTAPFLLSQAFAKHVKQGRIVNILDWRALRPGADHFPYTVTKSALA
                     AMTKSLAIALAPHITVNGLALGAILPPADQLSASEKIIEAVPVKRWSEAGEAEDALLF
                     LLSSSSAYITGEILHVDGGRHLV"
     gene            1509..2516
                     /locus_tag="PLG52_10065"
     CDS             1509..2516
                     /locus_tag="PLG52_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41834.1"
                     /translation="MNQNIPSYTGDIAAQQIDPTKEYSTENQEISFSIEQHAIWADLF
                     AGIHQPYLMEHICAEWKKGMDLLALSPKGIPTVQHLVEHITPRTGWKIERTVVRYTLA
                     DDWYKKFAQRVFLITDYLRTRDQMEFTPEPDMFHDIFGHLPFLTQEFYARIEDKFAPA
                     YIKATTEEREVIKRLAWYSTEFGLVVQDNRFTVFGAGIISGRKELTRTIMEFYRLGRD
                     NVIDYTKDVYQQLNQHFLANKSNLFKIIDGVNALHQKGQMSSADEGWNVVLGLYKSLG
                     IKPEGIFGGEVILVPFNLETISRIPKTVYAFNPMLFVCNSLEEMDEILDSFLKPIAMR
                     K"
     gene            2634..>2820
                     /locus_tag="PLG52_10070"
     CDS             2634..>2820
                     /locus_tag="PLG52_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41835.1"
                     /translation="MAATYNFQWQNPNLLKSIYPMRLQKLRDFLVFYKEADLWAEYKN
                     KDISTLAADVKEYEDGMD"
BASE COUNT          737 a          684 c          701 g          698 t
ORIGIN      
        1 caatatcgat tgggcgggga gcatatgtat tctcgccgcg tacacgtccc atttgtgctt
       61 cgatgggacg gttgatctca tacttgatat gttcagcatc aagtgaagtt agcaataaca
      121 cacagacgtt caagaaattc ggtccggcat agcccactga ctcggtttcc cataccgaag
      181 aaaccgctga aacctcccca acttttttta acaacgtgat cgcaagtgga atatttttct
      241 ctggctcaag gtttgagccg aggttcaagt agacgtagtg caagtcactc atcccgtttg
      301 cgttcgacct ccacgcccac agatttggca aagcgcaccg cacccggctt ctccacgcgg
      361 acaacgacct tgcgcaccaa cggcgtttca aggcagatct tcgccaagtc atttgccagt
      421 gcttccacag tcagccgttt tgccgtctct gcatgggatt gaatcttttt cgccaaggcg
      481 ctgtaatcga cacaatcttc gatatcgtcc gtttcggcgg cacgaagcgt gtcggtataa
      541 accgtcacat tgatgagaat gtcctgctcc ttttcacgct cccactcgcg gataccaata
      601 atgccacgca cgagcaggtc tttgataaat actttgtcca tgaaggtctc ctttacacta
      661 aatgcctgcc accatcgaca tgaaggatct cgccggtgat ataggcagaa ctggatgata
      721 gcaaaaatag taaggcgtct tcagcttcgc ccgcttcact ccaacgtttg acgggcacag
      781 cctcaattat cttttcactc gctgacagtt gatctgcggg cggcaggatt gcgcccaacg
      841 ctagcccatt gacggtgata tgtggcgcga gagcaatggc taatgatttg gtcatcgctg
      901 ccaaggcaga tttggtgacg gtgtaaggga agtgatccgc gccagggcgc aaggctcgcc
      961 aatcgagaat gttgacgatg cgaccttgtt tgacatgttt cgcgaatgcc tgacttaata
     1021 gaaatggcgc ggtcaggttg atgttgagat ggtagttcca atcgtcgaga gacgtttcct
     1081 gcaaggataa cggctcaaaa attgatgcgc tgttgactag tccatatagt ggactgagtt
     1141 cgttcgcgcg ttcgattaat tgcgatgcgc tttcagggtg agaaaagtcc gcagcgagga
     1201 cccaggcgcg gcgtccgaga gaggcgagtt catcccgaag tgattcggct tcttgaggcg
     1261 aatgtccatg atggaggatg aggtctgcgc cagcgcgtcc acaggcgaga gcgaataaac
     1321 gccccaagcg gcgggcggct ccggtgatga ggatggtctt gccggtcaga tcatacatgg
     1381 aatgattcta tcactgccaa atatgacaat tcttctccgc ttcttgactc gggtttttga
     1441 aggtgatact attttttcga taagttctgt atcccccaag tcctactcag aaaggtgtaa
     1501 aggtgaacat gaaccaaaac ataccaagtt ataccggaga tattgcagca caacagatcg
     1561 accccacaaa agaatattca acagaaaatc aggagatctc attttcgatt gaacagcatg
     1621 ccatttgggc agacctgttc gcaggcatcc atcagccgta tttgatggag catatctgtg
     1681 cagaatggaa gaaaggcatg gatctgctgg ctcttagccc gaagggtatc ccaactgtgc
     1741 agcatttggt agagcacatt acaccacgca caggttggaa gatcgaacga actgttgtga
     1801 gatacacgtt agccgatgat tggtacaaaa aattcgcgca acgtgtattt ttaattaccg
     1861 actatttacg cacccgcgac cagatggaat tcactcccga gccggatatg ttccatgaca
     1921 tcttcggaca tttaccattc cttactcaag aattctatgc acgcatcgaa gacaaattcg
     1981 cgcctgccta tataaaagcc accacagaag agcgcgaggt catcaagcgt ttggcatggt
     2041 acagcaccga gtttggcttg gtggtgcaag ataatcgctt tactgttttc ggcgcgggca
     2101 tcatctcggg acgcaaagag ttgacccgca ccatcatgga gttttaccgc ctcgggcgcg
     2161 acaatgtgat tgattacacc aaggatgtgt atcagcagct gaatcaacac ttcctcgcca
     2221 ataagagcaa cttgttcaag atcatcgatg gcgtgaacgc gcttcaccaa aagggacaga
     2281 tgtcctctgc ggatgaaggc tggaacgtcg ttttgggctt gtacaagagt ctgggcatca
     2341 aacccgaagg catctttggc ggagaggtca tcctcgtgcc gttcaatttg gagaccatct
     2401 cacgcatccc gaagacggtc tatgccttca accccatgct cttcgtatgc aattcgctcg
     2461 aagaaatgga tgagattctg gattcatttt tgaagccaat tgcgatgcga aagtaaattt
     2521 gagcaaactc ccgaagcagg cgcttcggga gtttgtattt aatctgcggt tgaaaaatcg
     2581 attaaattgg ctaaaatgga gtgagtattt acatctggca ccaggagaga gacatggcag
     2641 caacgtataa ctttcagtgg caaaacccca atttacttaa gagcatatat cccatgcgcc
     2701 tgcaaaaatt gcgggacttt ctggttttct acaaagaagc cgatctatgg gcagaatata
     2761 agaataagga tatttccacc ctcgccgccg atgtgaaaga atacgaagac ggcatggatt
//
LOCUS       DAOQNJ010000362         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26296_length_2820_cov_9.421700, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000362 DAOQNJ010000000
VERSION     DAOQNJ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26296_length_2820_cov_9.421700"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLG52_10075"
     CDS             <1..381
                     /locus_tag="PLG52_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41836.1"
                     /translation="FMLAMIALYGMGMMMASAFLMFGREAWHMANLAQEPIFLASGFY
                     FPIKSLPFWVAAGASVIPLTLGMDAMRQLIFPTGFKFGFLTVGTEIAILAVLSVVFVV
                     TAKVLLAYVEKLAVREGRITESRR"
     gene            459..1313
                     /locus_tag="PLG52_10080"
     CDS             459..1313
                     /locus_tag="PLG52_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41837.1"
                     /translation="MQTSTSWRSFRMAAWLGWVIESNWTDPFLFAVYSIVKPISGAAI
                     LVIMYGIISQGNFDSALFPYIYLGNAFYIYVGAVMTGVSWAVVDDREHYKTLKYMYIA
                     PIHIPLYLLGRGVARFVTGSIAVFITILAGVLFLNVKINILQVDWLLFFTTLIVGVVM
                     LALLGLLLAGLTLTLARHEGFIGEATAGALYIFSGAIFPLDVLPTWLRPIGYFMPTTY
                     WLELLRRSLVGNVAQAFPTLANFSNLQILGVMVGLSIFFGVISVYVFRWCDGIARERG
                     LIDRTTNY"
     gene            1397..2041
                     /locus_tag="PLG52_10085"
     CDS             1397..2041
                     /locus_tag="PLG52_10085"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNB41838.1"
                     /translation="MKLISINAGKKHTLVNKGKTEITGIYKNPLAGAVHITKLGIADD
                     YIGSPKHHGGPDQALYVYGEADYQWWEKEIGTKLGPGMFGENLTISELACSGFNIGDY
                     LYIGNVTLQVTAPRIPCGTFASKMEDPQWVKKFSAAERPGFYVRVLQEGELSASEEVR
                     VEKYTGATLSLVQVYRDHYDKEGRNEAVLRRHIDSPLSIRMREKYERELEALLH"
     gene            complement(2114..>2820)
                     /locus_tag="PLG52_10090"
     CDS             complement(2114..>2820)
                     /locus_tag="PLG52_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB41839.1"
                     /translation="ISQEKVGYWDATSMCGPLAWTIMRDVNAFPYRIGSWSQNAATFT
                     RVNPRWDGQPWGSFDPETFTLFHTEEAIAGYDFQSKGNLYTGDVVYTFATLYVTAGYF
                     DHIFVVAGVDENNTRLAISNMVQNSPYADCSIEEVKLYTPGDRETGVLNHEWNGNGYG
                     KTGTTGFDVFRWNWITYHNNGQAMSYTVRWGETIETIAFDWKISPESLQAANQFSADT
                     QLTPGQIIILPAPTQS"
BASE COUNT          656 a          603 c          760 g          801 t
ORIGIN      
        1 ttcatgctgg cgatgatcgc tttgtatggc atgggcatga tgatggcttc ggcatttctg
       61 atgtttgggc gtgaagcctg gcatatggcg aatttggcac aagaaccgat ctttctcgct
      121 tcgggctttt acttcccgat caagtcgctg ccgttttggg tggcggcggg ggcttcggtc
      181 attccactta ccttgggcat ggacgcgatg cgtcagttaa tcttcccgac tgggttcaag
      241 tttggctttt taactgtagg cacagagatc gcgattttgg cggttctttc tgtggtcttt
      301 gttgtgacgg caaaggtttt gctggcatat gttgaaaaac tggcagtccg cgagggaaga
      361 attacggaga gtcggagata aaagaagtgt caagtgtcag atgtcaagtt ccatgtaaaa
      421 cctgacacct gacacttgga acctgacacg gagaaaatat gcaaacttca acttcatggc
      481 gttcgtttcg tatggcggcg tggctgggct gggtgatcga gtctaactgg acagatccct
      541 ttctgttcgc ggtgtattcc atcgtcaagc cgatttcagg tgcggcgatt ttggtcatta
      601 tgtatggcat catttcacaa ggtaattttg atagtgcctt gttcccatac atttacttgg
      661 gcaatgcctt ctatatttat gtcggtgcgg taatgaccgg tgtctcttgg gctgtggtcg
      721 atgaccgtga acactacaag actctcaagt acatgtacat cgcaccgatt catattccac
      781 tgtatttgct tggtcgcggt gtggcgcgtt ttgtgaccgg ttccattgct gtgttcatca
      841 ccattttggc tggggtttta tttttgaacg taaagattaa cattctgcaa gtggactggt
      901 tgttgttctt tacgactttg atcgttggtg tggtgatgct ggcgcttctg ggcttacttt
      961 tggcgggtct aaccctcaca ctggctcgcc acgaaggctt tatcggcgaa gccaccgctg
     1021 gcgcgttgta tatcttcagc ggcgccatct tcccgttgga tgtattgcct acttggttac
     1081 gtccgatcgg gtattttatg ccaactacct attggcttga attgttgcgt cgttcgctgg
     1141 tagggaatgt ggcgcaagcc ttcccaacct tggcaaactt cagtaacttg caaattcttg
     1201 gcgtcatggt cgggttgagc attttctttg gggtgatcag tgtgtatgtc ttccgctggt
     1261 gtgacgggat tgcccgcgaa agagggctga tcgaccgcac aacaaattac taaaataatt
     1321 ccaaagtagg gttcgtgctg gcatggatgg gataaaatct gctcatgtca gcacgtttgt
     1381 atttaaagga tatagaatga aactaatcag catcaatgca ggaaagaaac acactctagt
     1441 caataaaggt aagacggaga ttacaggcat ctacaaaaat ccgctcgctg gagctgtgca
     1501 cattaccaag cttggaattg ccgatgatta tattggcagc ccaaaacatc acggcggacc
     1561 cgatcaagcg ctttatgttt atggcgaagc ggattatcaa tggtgggaaa aagagattgg
     1621 aacaaagttg ggtcccggca tgtttggaga gaatctcact attagtgaat tggcatgtag
     1681 cggttttaat attggagatt atctttatat tggcaacgtg acgttacaag tgacggctcc
     1741 tcgcattccc tgtgggacgt tcgcttcgaa aatggaagac ccgcaatggg tgaagaaatt
     1801 cagcgcagcc gagcgccccg ggttttacgt ccgcgtattg caagaagggg agctttcagc
     1861 gagtgaagaa gtccgtgtgg agaaatatac aggggcaacc ctttctttgg tgcaggtata
     1921 ccgcgaccat tatgataaag aagggcgaaa cgaggctgtc ctgcgaaggc atattgactc
     1981 acctctgtcc attcggatgc gggaaaaata tgaacgtgaa ttagaagctt tgcttcatta
     2041 agaagagaac agccaccata tggtggctgt tctcttcttg catgcttgcc caggaggggc
     2101 ccaaaattaa tttttatgat tgagtgggcg cgggcaaaat aataatctga ccaggagtaa
     2161 gttgtgtatc tgccgagaac tgattggcag cttgcaagct ttctggcgag attttccaat
     2221 caaacgcaat ggtttcaatc gtttcgcccc agcgaacggt atagctcatt gcttgtccat
     2281 tgttgtggta ggtgatccag ttccagcgaa aaacatcaaa gcctgtggtt ccagttttgc
     2341 cgtatccgtt tccattccat tcatggttga gaacgccggt ctcgcggtcg ccgggggtgt
     2401 agagttttac ttcttcaatc gagcaatctg catacggtga gttctgcacc atgttcgaga
     2461 ttgccaagcg ggtattgttc tcgtccacac cagcgacgac aaaaatatgg tcaaagtatc
     2521 cagcggttac atatagggtg gcaaaggtgt aaaccacatc gcctgtgtag agatttcctt
     2581 tagattgaaa atcataaccg gcaatggctt cttctgtatg gaagagagtg aaggtctctg
     2641 ggtcgaacga tccccagggt tgtccgtccc agcgggggtt cacgcgggtg aatgtcgcag
     2701 cgttctgtga ccaacttccg atgcgataag ggaaggcgtt tacatcacgc atgatcgtcc
     2761 atgccaaagg tccgcacatc gaggtggcgt cccagtagcc aactttttcc tgagagattt
//
LOCUS       DAOQNJ010000363         2817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26334_length_2817_cov_9.277335, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000363 DAOQNJ010000000
VERSION     DAOQNJ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26334_length_2817_cov_9.277335"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /gene="rimO"
                     /locus_tag="PLG52_10095"
     CDS             complement(<1..1022)
                     /gene="rimO"
                     /locus_tag="PLG52_10095"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNB41840.1"
                     /translation="MSKNTFHLVSLGCSKNTVDSDSMAQLLTRDGYSFVEDPSKASVL
                     IVNTCGFIGPAKDESYRVLGELAQGKKKNQVLIAAGCLTQRYGVEVAKKVPGIDGIIG
                     TRRWMDIVQVVEDARKGKHPEPIYHLPEAATVGADENGAMRVSVTGPSAYLKIADGCR
                     RPCAFCAIPLIKGTAVSRPVESIIHEARTLRDSGVRELILIAQDSTDYGHDLGMKDGL
                     ATMLEQLTDAVPDMDWIRIMYSYPGYVTDRMIEVMATRKQILPYLDIPLQHAHPKTLY
                     RMKRPSNMDWVHKTLAKMRATIKDLAIRTTFIVGYPGETDEEFQALYDFVEQTRFDRA
                     GAFQFS"
     gene            complement(1058..2746)
                     /locus_tag="PLG52_10100"
     CDS             complement(1058..2746)
                     /locus_tag="PLG52_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNB41841.1"
                     /translation="MQETIGQRLKREREARYLSLEKAAEETRIRRVFLQALEADDYSV
                     MPSAAQGRGFLRNYAEYLNINIDELIAEIQRNPIPPAEVSGPLPQVNLAETELPPLTE
                     AEDEKSAPPFWTRFLNRKPKEESTPEAPQEPSPEIEQILEEQPPAVVEEEKPKTRGRK
                     KKIVVEEAIVEPVAEVKVEEPESQPSVESQPDGQEQVEIQEEPKQAWWGRVVSLFKIK
                     VQKQDSAFEVVLSEDKEDIPQNGVSSIPDKPADVIMAEIGKMLRERRELISLTVDEVE
                     RHTHLRAVFVKALEEGAFDKLPSTVQTRGMLSNYASFLDLDVDTVLLRYADALQARRR
                     EKYAETPREKIQMQVNPSMPVLRTFIAGDLLFGVIMIAVIVALAIWGVGRLVSSDQQT
                     QVQPTSPSIVDVLGNLSDATASPAVTFVAVDDPAIANAGTAAEGTVTPAGSEVASTAN
                     VVVNVYALERVFVRISVDGEVAFEGRLAPRESRFFEANDQVVILTGNGSGLRITYNGE
                     DLGLMGGPGEVVNRVYLITGVVTPTATIPPTPTNTPLTTPTLTPTVTPTGTATP"
BASE COUNT          711 a          727 c          646 g          733 t
ORIGIN      
        1 aaggagaatt gaaaagctcc ggcgcggtcg aagcgggttt gttccacaaa atcatacaag
       61 gcttgaaatt cttcatccgt ctcaccggga tatccaacga tgaaggtcgt acgaatggca
      121 aggtctttga tggttgcgcg cattttggca agcgttttgt gaacccaatc catattcgac
      181 gggcgcttca tacgatacaa cgttttaggg tgagcatgtt gcaacggaat atccaagtac
      241 ggcaaaatct gtttacgagt tgccatcact tcaatcattc gatcggtgac ataacccggg
      301 taagaataca tgatacgaat ccaatccata tccggcaccg catcggttaa ctgttcgagc
      361 atagtggcaa gaccatcttt catgccgagg tcatgaccat aatcagttga gtcttgcgcg
      421 atcaaaatga gttcacgcac accgctatct cttaaggtgc gtgcttcatg gatgatggac
      481 tcaacagggc gcgaaactgc cgtgccctta atgagcggaa tagcacaaaa cgcacacggg
      541 cggcgacatc catccgcaat tttcagataa gcactgggtc ccgtcacact gacgcgcatc
      601 gcgccatttt catcggctcc tacagtagct gcttctggca aatgataaat tggttcaggg
      661 tgtttgccct tacgagcatc ttctacaacc tgcacaatgt ccatccaacg gcgagtgccg
      721 ataataccgt caatgcctgg cactttcttc gcaacctcca ccccatagcg ttgcgtcaaa
      781 catcctgccg caataagcac ttgattcttt ttcttgcctt gtgccaactc ccccagcaca
      841 cggtatgact catcctttgc cggaccaata aacccgcagg tattaacgat caagaccgaa
      901 gccttcgatg ggtcttcgac aaaactatac ccatcgcggg tcagcaattg agccatcgaa
      961 tcagagtcga cagtattttt tgaacaaccg agagaaacta aatgaaaagt atttttagac
     1021 acgtttgatc ctgacaataa attgatgaaa ctaagaatta cggagttgcc gttcctgttg
     1081 gagtgacagt cggcgttaag gttggggttg taagcggcgt attcgtgggt gtcggcggga
     1141 tagtcgccgt aggtgtgaca acaccggtaa tgagataaac ccggttcacc acttcgccgg
     1201 gtccgcccat cagaccaaga tcttcaccat tatatgtgat tctcagccca gaaccattcc
     1261 cagtcagaat aacaacctgg tcgtttgcct caaagaaacg gctttcgcgc ggtgccaaac
     1321 gtccctcaaa ggcaacctca ccatctacag aaatacgaac gaacacgcgc tccaatgcgt
     1381 atacgttcac aactacgttt gcagtcgaag caacctcaga acccgccgga gtaactgtgc
     1441 cttctgcggc tgtgccagca ttggcaatgg cagggtcatc taccgcaaca aaggtcactg
     1501 caggtgaagc cgtcgcatca gacaaattgc ccaacacatc cacaatagat ggagacgttg
     1561 gctgaacttg tgtttgctgg tctgagctaa ccagccttcc aacgccccaa attgccaaag
     1621 caacaatgac cgcgatcatt attacgccaa aaagcaaatc gcctgcaata aaagttctca
     1681 acaccggcat agacgggttg acttgcattt ggattttttc gcgcggggtc tctgcatatt
     1741 tttcacggcg tcttgcttgc aaggcatcgg cataacgcaa aaggacagtg tctacatcca
     1801 gatcaagaaa actggcatag tttgaaagca tgccacgagt ctgcacggtg gaaggcagtt
     1861 tatcaaaagc accttcttcc aaggctttca caaacaccgc acgcaaatgc gtatgacgct
     1921 ccacttcatc gacagttaag ctgatgagtt cacgccgctc gcgtagcatc ttaccgatct
     1981 ctgccataat gacatcagcc ggcttatcag gaatggacga aacaccgttc tgcggtatat
     2041 cttccttatc ttctgaaagc acaacctcga acgccgagtc ttgcttttga acttttatct
     2101 tgaataacga aacaactctc ccccaccaag cttgcttcgg ctcttcctgg atttcgacct
     2161 gttcctgtcc atctggctga ctctccacag atggctggga ttcgggttct tccactttta
     2221 cttcagcaac tggttcaact attgcttctt caacgacaat ttttttcttc ctgccgcgtg
     2281 ttttcggttt ttcttcttca actactgctg gaggttgctc ttccagaatc tgttcaattt
     2341 caggagaagg ttcttgaggc gcttctggcg tagactcttc tttgggtttg cgattcagga
     2401 agcgggtcca gaaaggaggc gcggattttt catcctctgc ttccgttaat ggaggaagtt
     2461 ccgtctctgc taaattgact tgaggaagtg gtccactaac ttctgcgggt ggaatggggt
     2521 tgcgctgaat ttcagcgatg agttcatcaa tattgatatt gagatactcg gcatagttgc
     2581 gcaaaaaacc acgcccctgc gcggcagacg gcatgacgga ataatcatct gcttcgagag
     2641 cttggaggaa gacgcgccga atacgtgttt cttctgccgc tttttcaagc gagagataac
     2701 gtgcttcgcg ttcgcgcttc aatcgctgac cgatggtttc ttgcatggaa gtattgtatt
     2761 ataaacatca ccgacttgcg ccggtgatgt tttgacattt ttgttattcc gctttgg
//
LOCUS       DAOQNJ010000364         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26369_length_2815_cov_10.569928, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000364 DAOQNJ010000000
VERSION     DAOQNJ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26369_length_2815_cov_10.569928"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLG52_10105"
     CDS             <1..702
                     /locus_tag="PLG52_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41842.1"
                     /translation="QIIFTNGQSVILFHQESDGSWKSPLLGDMTMEDFEEWLQNSNDN
                     ETKKYYDEGVAKLLEATASPTEPPTEVPTDVPPTEVSSVNGQGETSNACSQLQEPIDG
                     RALNNAGKVNFAWSEQPNAQTYIITFIKEDGSTARIETSSNSAEFYIEVLPAGGNYQW
                     FVTAYGADGKEICKSNSANFSKPQAEPTEKPKATKDPNAEEPASGTAGCPCDPYDYLN
                     YSDCVGSHMDVCSIP"
     gene            730..2631
                     /locus_tag="PLG52_10110"
     CDS             730..2631
                     /locus_tag="PLG52_10110"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB41843.1"
                     /translation="MSKNNNPLNLRLILVFGTVALLLIVQVLGLFSTITTPIERIEYS
                     MQDTFVRLRGAEKPSGEIVIVAIDDQSFNWTGLQWPWPRSYFAEIVDQINKGGGKVVG
                     MDLILFEPDEKQPENDLVLAKALGESPAAVAVMQINKTGGEGYATETLSQPLSAYRDV
                     LDGIGTTDVKRDEDAIVRNVSAYSIYQGQVYPHWAFELAKLYLNAEQPIFGNSSVKFN
                     GQNIPLRSNELPIYFAGPAGTYPTYSASDVHDGITLEQNPDAFRGKIVLIGATTITLQ
                     DIYPTPFSATDPTPGVEVVANTVDTIINGKFITYAPPWLALLLIIVAAGMAYLITLSK
                     RPSFTLTALGISILLYLVAAYFVFSSQRYILPTIAPTLMLFLGVILPTLEQAVSQEQE
                     KRRVRNLFSRFISPEMVDQMMKTQDINSLNKRADLSIIFSDIRGFTTLSEKMTPEGVV
                     ALLNPYLEAMSQVIYKHGGTVDKYEGDAIIAFFGEPVPNKDHAVRALRASMDMRKALA
                     ELRDKWAKEGRPSQIEMGVGINSGEVFVGLLGSAQRINYTVIGDNANLASRLQDLTKN
                     YAWPILISESTYQQVKDEFDCELADAVTVKGKTKAVNVYKVIGEKGAPESEQLQAWTS
                     DQVKPKHTS"
BASE COUNT          812 a          667 c          621 g          715 t
ORIGIN      
        1 cagatcattt ttaccaatgg gcaatctgta attcttttcc atcaagaatc cgatggttca
       61 tggaagtctc cattacttgg ggatatgacc atggaagact ttgaagaatg gctccagaac
      121 agtaatgaca acgaaacaaa gaaatactat gatgaaggag tagcaaagct tttagaagcc
      181 actgcatctc ctacggaacc gccaactgaa gttccaaccg atgtaccgcc taccgaagtc
      241 agttctgtca atggacaagg tgaaacaagc aatgcgtgtt cgcagttaca agagccaata
      301 gatggccgtg cgctcaataa tgctggaaaa gtcaattttg catggagtga acaaccgaac
      361 gcgcaaacct acattattac atttataaaa gaagatggca gcaccgctcg aattgaaacc
      421 agttcaaata gcgcagaatt ttacattgag gttttgcctg ctggtggaaa ctatcaatgg
      481 tttgtcacag cctacggcgc agacggaaaa gaaatttgta aatccaatag tgccaatttc
      541 tcaaaaccgc aagcagaacc aactgaaaag ccaaaagcaa ccaaagatcc aaatgcagaa
      601 gagcctgcta gtggtaccgc tggctgtcct tgtgacccct atgattactt gaactattcc
      661 gattgtgttg gctctcacat ggatgtttgt agtattcctt aatcatctca agctatcaaa
      721 ggataagaca tgtcaaaaaa taataatccc ttaaacttac gccttattct ggtatttggg
      781 acagttgcac tactcttaat tgttcaagtt ctgggattat ttagcaccat caccacaccc
      841 attgagcgga ttgaatactc catgcaggat acatttgtgc gcctgcgtgg agcggaaaaa
      901 ccatctggtg agattgtcat tgtagcaatt gacgaccaat cttttaactg gaccggcttg
      961 caatggcctt ggcctcgctc ctattttgca gagatcgttg accaaatcaa taaaggcggc
     1021 ggcaaggtcg ttggtatgga tttaatcctt ttcgagcccg atgaaaagca acccgaaaat
     1081 gacctggttc tcgcaaaagc actcggagag agccccgccg cagttgctgt gatgcagatc
     1141 aataaaacgg gtggagaagg ctatgcaacc gaaaccctgt ctcagccatt atctgcgtac
     1201 cgtgatgttc tggatggcat aggcacaaca gatgtaaaac gcgatgaaga cgccatcgtg
     1261 cgaaatgtat cggcatacag tatctatcag ggacaggttt atccgcattg ggcatttgaa
     1321 cttgcaaagt tatatttgaa tgctgaacaa ccaatatttg gaaattcaag cgttaaattt
     1381 aacggtcaaa acattccgtt acggtccaat gaacttccta tctattttgc aggacctgca
     1441 ggaacctacc caacctattc tgcatcagac gttcacgatg gcatcacgtt ggaacagaac
     1501 ccagatgcct tccggggcaa aattgtcttg attggcgcga cgacgattac attacaggat
     1561 atttacccaa cccccttttc ggcgacagat ccaacacctg gtgtggaagt cgttgcgaac
     1621 acggtagata caatcatcaa tggcaagttc attacctatg ccccaccttg gttggcactt
     1681 ttgctaataa tcgtggcggc tggtatggca taccttatca cgctttcaaa gcgcccgtct
     1741 tttacactca ccgctttagg cattagcatt cttctctatt tggttgctgc gtattttgtt
     1801 ttctccagtc aacgttatat attgccaacc attgcgccaa cccttatgct tttccttggc
     1861 gttatccttc ccacattaga gcaggcagtt tctcaggaac aggaaaagcg ccgtgttcgt
     1921 aatctattca gccgtttcat ttctccagaa atggttgacc agatgatgaa gactcaagat
     1981 attaattcac tcaacaaacg tgcagatctt tccattatct tttcagacat tcgcggcttc
     2041 acgacacttt ctgaaaaaat gacgccggaa ggagtggttg ccctgcttaa cccctacctc
     2101 gaagccatgt cgcaggtcat ctataaacat ggcggaaccg tagataaata cgaaggcgat
     2161 gccatcattg cgttctttgg cgaacccgtt cccaataaag accatgcggt tcgtgctctg
     2221 cgcgcctcaa tggatatgcg caaagcattg gctgagcttc gagataaatg ggcaaaagaa
     2281 ggtcgcccca gccagatcga aatgggagta ggcatcaact cgggcgaggt gtttgtaggg
     2341 ctgcttggct cagcacaacg catcaactat acggtcattg gcgataatgc caaccttgcc
     2401 tctcgcctgc aagacctcac caaaaactac gcctggccca ttctcatcag tgaaagcacc
     2461 tatcaacaag tcaaggatga attcgattgc gaacttgcag atgcagtcac cgtcaaaggg
     2521 aaaaccaagg cggtcaatgt atacaaggtc attggcgaaa aaggcgctcc agaaagcgaa
     2581 caactacaag cctggacaag cgaccaggtg aagcccaaac acacatcatg acgcaaaaac
     2641 ggagcgagaa ttctcgctcc gtttttgtac cggctcaggc gtagacgcag gcttggtctg
     2701 gcaaccaggc agaaatccac gctggaggcg gatttttaat cccctgttta tttgtgaaag
     2761 acttgtacat aattgtacaa aaagcgtttt tttggtatag ttatggctat actac
//
LOCUS       DAOQNJ010000365         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26600_length_2800_cov_6.852459, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000365 DAOQNJ010000000
VERSION     DAOQNJ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26600_length_2800_cov_6.852459"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLG52_10115"
     CDS             <1..230
                     /locus_tag="PLG52_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB41844.1"
                     /translation="GSLRRTDLRPSLHTIQAPVMGMYGDKDIVVNPNQWKPLLDGVSK
                     ARIERFHTAGHFIMLDEPITFNQKLKSFLDE"
     gene            242..853
                     /locus_tag="PLG52_10120"
     CDS             242..853
                     /locus_tag="PLG52_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNB41845.1"
                     /translation="MSETYHYPQKMGRIVLLGMEEVIGKGGMDAVLNFAALNDLQPSA
                     TNERTFSFETVSKLHQSLEQVYGPRGGRGLALRSGRACFKYGIKEYGSLLGLTEMAFR
                     LLPLPTKLHTGARSFADLFNKHTDQQVRVEETESQILWHIERCPLCWERKTEEPVCHL
                     AVGLLQEALYWLSGGKVFSVEETACHARGDEHCIITIQKTPLT"
     gene            922..2199
                     /locus_tag="PLG52_10125"
     CDS             922..2199
                     /locus_tag="PLG52_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41846.1"
                     /translation="MLKIILHAPNPIMPFCEPARELRIQNTPLWLHQRNLLAPYVTRE
                     MELKQGERLQPVREPAIVYRDNLFFDEAFITEFMTQALKLNRPVRAAFRADDPAFREH
                     ALPLSTSYTPAGSLYLADLWYYPNGPVAGAEPLVIDMQAKEIGYYHVPTYMADQSGDL
                     VFQVPLRCMIAIDSWVHIFISDMVFSQFARGARFEKRLNEDLMFKLKILGKAVYEGRQ
                     VLECSELVKVGKGCVIDPSAVIHGPTIIGDNVTINAGAVIENCVIGSNVNVSQDVQLM
                     LSVVGDGAFLPFRTALFMTTVMENSMVAQNTCLQMCVVGRNTFIGAGSTFTDYNLIPA
                     PLRARDGNGNLSISNRPVLGGAVGHNCRIGSGMVIYPARTIESDVVLAASTERRVIDR
                     DITFDQSDHHKLKLAHLHQTPYHRNRKAEVESW"
     gene            2419..>2800
                     /locus_tag="PLG52_10130"
     CDS             2419..>2800
                     /locus_tag="PLG52_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41847.1"
                     /translation="MMQLPERTVYRKIIQTGRAAEELGANILGLGAFTSVVGDAGVTI
                     ANSLDIPVTTGDSYTVMMAVQAIREAAKVMDIKIEDATVAVVGATGSIGRVCAELIAG
                     EAARTLLIARDEKKLEDLRDRLKVQ"
BASE COUNT          666 a          647 c          747 g          740 t
ORIGIN      
        1 ttggctccct acgccgtacg gacttgcgcc caagcttgca taccattcaa gcccctgtga
       61 tggggatgta tggtgacaaa gacattgtcg taaacccaaa tcagtggaag cccttattag
      121 atggtgtttc aaaagcgcgg attgagcgct ttcatacagc aggtcacttc attatgctgg
      181 atgagccgat tacattcaat caaaaactaa aaagctttct ggatgaataa atacaaaact
      241 catgagtgaa acctatcatt acccccaaaa gatgggaagg atcgtccttc tcggtatgga
      301 ggaagtgatc ggcaaaggcg ggatggatgc cgttctcaac tttgcagcct tgaatgactt
      361 acagccgtcc gctaccaatg agcggacgtt ttcgtttgaa acggtgagca agttgcatca
      421 atcgctggag caggtttatg gtccgcgtgg tgggcgtgga ttggcgctcc gttcggggcg
      481 tgcttgtttc aagtatggga ttaaagaata cggctctcta cttgggctga ctgaaatggc
      541 gttccgttta ttgccgctcc cgaccaaact gcatacaggt gcccgttctt ttgcagacct
      601 tttcaataaa cataccgacc agcaggtgcg tgtagaagaa actgagtccc agattctttg
      661 gcatattgag cgctgtccgc tgtgctggga gagaaagacc gaagaaccgg tttgtcattt
      721 ggcggtgggt ttgcttcaag aggcgttgta ctggctgagt gggggcaagg tttttagcgt
      781 ggaagagaca gcttgtcacg ctcgggggga tgaacactgc attattacca ttcagaagac
      841 ccctttaacc taaaattgca taacaatttt gttttcttac atagcgtgcc gagaggcacg
      901 ctatgattta taattctgcc catgcttaag attattcttc atgcgcccaa cccgatcatg
      961 cctttttgcg aaccggcgcg tgaacttcgg attcaaaata ctcctctttg gcttcatcag
     1021 cggaatttgc tcgcccctta tgtgacccgc gaaatggagt taaagcaggg tgaacggctc
     1081 cagccggtac gtgaacctgc cattgtttac cgtgataact tattcttcga tgaggcattt
     1141 attacggagt tcatgacgca ggcattgaag cttaaccgcc cggttcgagc ggcttttcgc
     1201 gcagatgacc cggctttccg tgagcatgct ttgcctttgt ctacatccta taccccggcg
     1261 ggtagcttat atcttgcgga cctttggtat tacccaaatg gtcctgtagc gggtgcagag
     1321 ccgcttgtca ttgacatgca ggcgaaagaa attgggtatt accacgtacc aacctacatg
     1381 gcagaccaaa gtggcgacct ggtttttcag gtgccgttgc gttgtatgat cgccattgat
     1441 tcatgggtgc atatttttat ttcagacatg gtgtttagcc aatttgctcg tggcgcgcgc
     1501 tttgaaaaga gattgaacga agacctgatg ttcaaactaa agattcttgg caaagccgtt
     1561 tacgaaggtc gacaagtttt ggaatgttca gagttggtaa aagtagggaa gggctgtgtc
     1621 attgacccaa gcgctgtgat tcacggaccg acgattattg gagataatgt caccattaat
     1681 gcgggggctg ttattgaaaa ttgtgttatt ggtagcaatg tcaatgtctc gcaagatgtg
     1741 cagttgatgc tttcggttgt gggagatggt gcatttctcc cattccgtac agctttattc
     1801 atgaccactg tgatggaaaa tagcatggtg gcgcaaaata cttgtttgca aatgtgcgtg
     1861 gtgggacgta atacgtttat cggcgcaggc tctacattca cagattacaa cttgattccc
     1921 gctccgctcc gcgcccgaga tgggaatggc aacttgagca tttccaaccg cccggtgttg
     1981 ggtggggcag tggggcacaa ctgtcgtatc ggttcgggga tggttatcta cccggcacgc
     2041 accatcgagt ccgatgtagt gctggcagct tctaccgaac gacgtgtgat tgaccgcgat
     2101 attactttcg accagagtga ccatcacaaa ctgaagctgg ctcacctgca ccaaacaccc
     2161 tatcatcgca accgcaaagc agaagttgag tcatggtaaa tggatagttt cgccttcatc
     2221 attcacccca tcgatcccaa gcgcgacgtt agccgaaaat acccgttttt agggaaggtt
     2281 cttaccgaga agcagatcga ctttttttcc acctttttcc ccccggttta tatctctgaa
     2341 atcgagggaa ttacctcaaa ggcgaccgga aagcagatta aaggctggtt ccttgcatgt
     2401 ccgtatactc caaggcgtat gatgcagttg cctgagcgca cggtctatcg taaaattatc
     2461 cagacaggtc gtgccgccga agaactcggt gccaatatcc tcgggcttgg ggcgtttacg
     2521 tcggtggtgg gggatgccgg tgtgacgatt gccaacagtc tcgatattcc agtcactact
     2581 ggagactctt acacggtcat gatggcggtt caagccattc gtgaagccgc taaggtgatg
     2641 gatatcaaga tcgaggatgc cacagttgct gtggtgggcg ccacgggttc gatcgggcgg
     2701 gtctgtgcgg agctgatagc tggtgaagcc gcccgaacct tactaatcgc ccgggatgag
     2761 aaaaagcttg aggatctgcg agacaggctc aaggtccagg
//
LOCUS       DAOQNJ010000366         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26619_length_2799_cov_6.400875, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000366 DAOQNJ010000000
VERSION     DAOQNJ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26619_length_2799_cov_6.400875"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLG52_10135"
     CDS             <1..336
                     /locus_tag="PLG52_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HNB41848.1"
                     /translation="RNNIENPRNTICIVSWQAPNTMGRRLADREPEIRIFGEAYKLKC
                     EVATIGGLSGHAGQDLLVEYATSVKQSVKDVFLVHGEENQATTLTALLKERGMDKVHY
                     PELHDSVDV"
     gene            373..459
                     /locus_tag="PLG52_10140"
     tRNA            373..459
                     /locus_tag="PLG52_10140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:407..409,aa:Ser,seq:cga)
     gene            538..1770
                     /locus_tag="PLG52_10145"
     CDS             538..1770
                     /locus_tag="PLG52_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41849.1"
                     /translation="MKRYSIHWVWIFVVILSACGGGTPSAPSTDVQPLASPTVLPETE
                     TVSTELPKSVASVEECKLPPLSFTNVGLGLPNPAFKMPSVGDVKTIVLFADFSDVPAT
                     QTPEELLSIISPGAEAFYSEMSYGRMRWTLEPHPVWLRLSQPSAHYGEGIRSYDGHLA
                     FIQEAVTLADADVDFSTADSVIVLVPPEASAIPYGPAFGASPGGGYFADGKTFANGAT
                     SGADLPGWGSYWLNHETGHMMGLPDLYAYQYDPNNYPDQHRFVGDFSLMGFIDGSAPG
                     FFAFERWQLNWLDDEQIVCQTTMDETTTLTPIETQGGIKAVIVPISESKALVVESRRP
                     VGADAELVKSGALVYLADTSIYSGEGTITVLPVSGDDPYRVQSPLAVGETFTVEGITV
                     TVTASDDQSDTVQVVVTK"
     gene            1806..2657
                     /locus_tag="PLG52_10150"
     CDS             1806..2657
                     /locus_tag="PLG52_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNB41850.1"
                     /translation="MKLKLALAQINTKLGDVEANLAKHLEYIERAKAEKADLLVFPEL
                     SLTGYVLQDLVASVAHKPTEDDPVFRHLLKASHDLDLVVGFVDEDSRHRFFIASAYLS
                     GGKVLHVHHKVYLPTYGLFDEGRFFAWGDSVRSFDTKFGRVGMLICEDFWHASPPYLL
                     WLDGADIMLFSSASPGRGLNDKDKLESARWVERITKAYASMFTSFVAHANRVGYEDGL
                     HFWGGATIFDPTGEELAHGPYNEEAITYSELDLNQLRRTRARLPLLRDERTALVQKEM
                     NRILARD"
     gene            2650..>2799
                     /locus_tag="PLG52_10155"
     CDS             2650..>2799
                     /locus_tag="PLG52_10155"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthetase"
                     /protein_id="HNB41851.1"
                     /translation="MIDINLSINTDLAREILSGFIKSEITRIGMSRAVIGLSGGIDSA
                     LSLALA"
BASE COUNT          673 a          642 c          710 g          774 t
ORIGIN      
        1 cgtaataaca ttgaaaatcc gcgcaacacg atttgtatcg tttcgtggca ggctccaaat
       61 acgatgggac gtaggctggc agatcgtgag ccagaaattc gtatttttgg agaggcgtat
      121 aaacttaaat gtgaagtagc aaccatcggt gggttatcgg gtcatgccgg gcaggatttg
      181 cttgtagagt atgctacgtc tgttaagcag agcgtgaaag atgttttcct tgttcatggt
      241 gaagaaaacc aagcgaccac attaaccgct ttattgaaag aacgcggtat ggataaagtc
      301 cattaccctg aactgcatga ttccgtagat gtctaaaaaa attcgggtat aatcgcgtcc
      361 gcttcaaaaa ctggggaggt gccagagtgg ttgaatggga cagtctcgaa aactgttgtg
      421 ggctccggtc caccgagggt tcgaatccct ccctctccgc agtagcccgg cttacttaag
      481 ctgggctttt gtttttcata tcatagatgg atgatcagat ttataggaga tgcagcgatg
      541 aaaagatact caatacattg ggtttggata ttcgtggtca tactgtctgc ttgtggaggt
      601 ggaacacctt cggctccttc taccgacgtg cagccgcttg cttctccaac ggttctacct
      661 gaaacggaaa ctgttagcac agaattgcca aagtcagtag cttctgtgga agaatgtaag
      721 ttgccgccac tttcctttac caatgttggc ttgggattgc cgaacccagc attcaaaatg
      781 ccgtcagtcg gcgatgtaaa gacgattgtc ctctttgccg atttttcgga cgtgcctgcc
      841 acgcaaaccc ctgaagaatt actaagcatc atttcacccg gtgcagaggc gttttattcc
      901 gaaatgtcgt atggcagaat gagatggacc cttgaaccgc atccggtttg gctgcgattg
      961 agtcagcctt ctgctcatta tggtgaaggc attcgaagtt atgacggtca tttggcgttc
     1021 attcaagaag cggttactct tgcggatgcg gatgtcgatt tctctacagc tgattctgtg
     1081 atcgtgttgg tgccacctga ggcaagcgcc atcccgtatg gacctgcctt tggtgcatcg
     1141 cctggcggcg gctattttgc tgatggcaag acttttgcca atggtgccac gtctggcgca
     1201 gatttgccgg gttggggatc atactggttg aaccatgaaa ccgggcatat gatggggttg
     1261 ccagatcttt atgcatatca atatgacccc aataattatc cagaccagca tcgctttgtg
     1321 ggtgatttta gtttgatggg ctttattgat ggtagtgcgc cgggtttctt tgcttttgaa
     1381 cgctggcaat tgaattggtt ggatgatgaa caaatcgttt gccagacgac catggatgaa
     1441 acaacgaccc tcacgccaat tgaaacacaa ggcggcatta aggcagtcat cgtacctatc
     1501 agcgaaagta aagctctggt ggttgaatcc cgccgtcccg ttggtgcaga tgcggaactt
     1561 gtcaaatcag gggcattggt atatttggca gatacatcta tttattctgg cgaagggaca
     1621 attacggtat tgcctgtttc aggggatgac ccgtatcggg tgcaatctcc tttggctgta
     1681 ggtgaaacgt ttacggtaga aggaattact gtgactgtga ccgcttcaga cgaccagagt
     1741 gataccgttc aggtcgtagt aacaaaataa ttttgcttgt attttttagt ggtaagattc
     1801 tcgcaatgaa acttaaactc gctctcgcac agatcaatac aaagcttggc gatgtggaag
     1861 ctaacctcgc taaacacctt gaatacattg aacgcgcaaa agccgaaaaa gcggatttac
     1921 ttgtttttcc agaactttcc cttaccggtt atgtcttaca agatttggtc gcttcggtgg
     1981 cacacaaacc aacagaggac gaccctgtct tcaggcacct cctcaaagcg tctcatgacc
     2041 ttgacttggt ggttggcttt gtagacgaag attcacgtca tcgctttttc attgcctctg
     2101 catacctttc gggtggaaaa gttttgcatg tgcatcacaa agtatattta ccgacctatg
     2161 gactttttga cgaaggtcgt ttctttgcgt ggggagactc tgtccgctcg tttgatacga
     2221 agtttggacg agtgggcatg cttatctgcg aagatttttg gcacgcttcg cctccatact
     2281 tgctctggct tgatggcgcc gacattatgc ttttctcttc cgcctcgccg ggtcgcggac
     2341 tgaacgacaa agacaaactc gaatccgcac gctgggtgga acgcatcacc aaagcctatg
     2401 ccagtatgtt cacctcgttc gtagcgcatg ccaaccgagt gggttatgaa gatggactgc
     2461 acttctgggg aggggcaacg atctttgacc caacgggaga agaactggca catggaccct
     2521 ataacgaaga agccatcaca tattccgaat tggatttgaa tcaacttcgc cgcacacgcg
     2581 cccgcttgcc tttgttgcgg gatgaacgca cggctctcgt ccaaaaagaa atgaacagga
     2641 ttctcgcccg tgattgacat taacctcagc atcaatacag acctggctcg cgagattctc
     2701 tctggtttca tcaaatctga aattacccgc attggaatgt cacgcgcggt catcgggctt
     2761 tctggcggta ttgactcggc gctttctctt gcacttgct
//
LOCUS       DAOQNJ010000367         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26652_length_2797_cov_8.085704, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000367 DAOQNJ010000000
VERSION     DAOQNJ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26652_length_2797_cov_8.085704"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..>2797)
                     /locus_tag="PLG52_10160"
     CDS             complement(224..>2797)
                     /locus_tag="PLG52_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB41852.1"
                     /translation="EAGTLKPSEQLAETLLKLLGIPDLERPAWVRFARGLGESPAPAQ
                     FSQTNLPSQLTSFIGRKKELSEIKDALSKYRLVTLTGSGGIGKSRLSLQVGTDLLSTF
                     PDGVWFIELASLSDPSLLLHTIQNTLGLIDQKNQPALLALQEHLKEKKALFILDNCEH
                     LIEACAKLAFDLLRQTTHLKILASSREALSMHGEFAWRVPSLSLPDANSITEAEQLSQ
                     YEAVNLFIERAQLINPNFKLDENNASSIAQICSRLDGIPLALELAAARLKTLSVEQIH
                     TRLDDRFNLLTNGARTLPHRHQTLQATIDWSYNLLSENEKVLFQRLSIFSGGWSLESA
                     ESVCSGNGIHKKEVLDLLTSLAQKSLIQPYETPSGIRYEQLETIRQYGQEKFLASGEA
                     DILRKQHLQYFIELANQSEPELRGPAQMKWFNLLGQEMGNLRHALSWAQETDKGAFLQ
                     LASSLWSLFRSLEHKDEGLAWLSKAVEENKDTQTELLSTAMARLSYLYLYLSIVQEQA
                     EGYAITALGLGRQLKDKYAEALALISLAGLELERMNGKFGLEQTEQALEIARNLDDHW
                     LIATALIQKARFTQVKSQVESMEVFEEALREAQSSGDKRLIYSGLFWMFINLLATGQF
                     IRAHEVAHQCIILTDEIGDKDGIIYSHAALAGMGLYEENYPASIEHAETVIRLSKAYH
                     HNSGLLNGLGTAALANLAMKNYPQTLSLSDEMERLTVLKGGRLNTDTGYHVFLRVWVY
                     ILAGDMECARKQAKELIRLYQLDNNFLLNIEYFRAFAALAFVDGDHASHITLTSFGMK
                     LRERAVAAFFDHPFMIRLRGEQVAKSREILGEEAFNRAWEEGQKITMEQTKGWVAALM
                     R"
BASE COUNT          693 a          649 c          673 g          782 t
ORIGIN      
        1 gtcactgggc tgaggattgg atcgagcagt tggcaaaaga agggattaca tccggatgcg
       61 gtggcggcct gtactgcccg gataaccctg taacgcgtga tcagatggcg gtgttcctgg
      121 tcaaggcttt taatttaccg taagaacgcc tttcgaaaaa acgaatagtg ccgcttcaaa
      181 tgaagcggca ctatttttca actttcggga atcttcacgg gttctatctc attagtgccg
      241 caacccatcc ttttgtctgt tccatagtta tcttttgccc ctcttcccat gcgcggttga
      301 acgcttcttc tcccaatatt tcacgtgact tggctacctg ttcgcctcga agcctgatca
      361 taaagggatg gtcaaagaac gcagcaacgg ctcgctcgcg caatttcatt ccaaaacttg
      421 tcagcgtaat atgtgatgcg tgatcgccat ccacaaatgc cagtgctgca aaggctcgaa
      481 aatattcaat attcaacagg aaattattat ccagttgata aagccttatg agttctttgg
      541 cttgttttct tgcacattcc atatcacccg caagaatata aacccaaaca cgcagaaata
      601 catgataccc cgtatctgta ttaaggcgac cgcctttcaa gacggtcagc ctttccattt
      661 catccgacaa actgagcgtc tgcgggtagt ttttcattgc aaggttcgcc agtgcagcgg
      721 ttccaagacc gtttaataga cctgaattat gatgataggc ttttgacaaa cggatgacgg
      781 tctctgcatg ttcaattgaa gctggataat tctcttcata caaacccatg cccgccaaag
      841 cagcatggga ataaataata ccgtccttat caccgatttc atcagtcaat atgatgcact
      901 gatgggcaac ctcatgtgcg cggataaact gacccgtcgc aagcaaattg atgaacatcc
      961 aaaataaacc ggagtaaatc aggcgtttgt ctcccgatga ttgggcttcc cgcaatgctt
     1021 cctcgaagac ctccatgctt tcgacctggc tttttacctg ggtaaatctc gccttctgga
     1081 tcaatgccgt tgcaatcaac cagtgatcat caagatttcg ggcaatttcc aaagcctgtt
     1141 ctgtctgttc aagcccaaac ttcccattca tacgttccaa ttccagacct gccagggaaa
     1201 ttaatgctag ggcttcagcg tatttgtcct ttaattgcct gcccaaacca agagctgtaa
     1261 tagcgtagcc ttctgcctgc tcctgtacga tagaaagata taaatagaga taggaaagcc
     1321 tagccatggc agtagaaagt aattcggttt gtgtatcttt gttttcctct acagcttttg
     1381 acaaccatgc gagtccttca tctttatgtt ccaatgacct aaaaagcgac cacaaggaac
     1441 ttgcaagttg aagaaatgct cccttatctg tttcctgtgc ccagctcaag gcatggcgga
     1501 ggttgcccat ttcttgtcct agtaggttga accatttcat ttgagccgga ccacgcaatt
     1561 ctggttcgga ttggtttgcc aactcaataa aatattgcag atgttgtttg cgcagaatat
     1621 ccgcctcgcc actggcaaga aatttttcct gcccatattg gcggatggtt tccagttgtt
     1681 cgtagcggat tccgcttggg gtttcatagg gttgaataag ggatttttgt gcaaggcttg
     1741 taagtaaatc taaaacttct ttcttatgga tgccattccc tgagcaaaca gactcagcag
     1801 attctaaact ccatccgcca gaaaaaattg acaacctctg gaataaaacc ttttcgttct
     1861 ctgataataa gttgtagctc cagtcaatag ttgcctgtag ggtttggtga cggtgtggaa
     1921 gtgtgcgcgc gccattggtg agcaggttga aacggtcgtc gagccgggtg tggatctgct
     1981 ccacgcttaa agtttttaac cttgcggcag ccaactcaag ggcgagggga attccgtcga
     2041 ggcgggagca gatttgagca atggaagagg cgttgttctc atccagcttg aagtttggat
     2101 tgatcagttg cgcccgttcg atgaaaaggt tcaccgcttc gtattgggac agttgttctg
     2161 cttctgttat gctgttggca tctggcaggg acagtgaggg gacgcgccag gcaaattcgc
     2221 catgcatgct caatgcttcg cggctggatg ccagaatctt taggtgggtt gtttgtctca
     2281 atagatcaaa agctaatttt gcacaagcct cgatcaggtg ttcgcagtta tccaatatga
     2341 ataaggcttt cttttccttt aaatgctctt gtagtgccag taaggcaggc tgattttttt
     2401 ggtcgatcag ccccaaagta ttttgtatgg tgtgaaggag aagacttgga tcggagaggg
     2461 aagctaattc gataaaccaa acaccatcgg gaaaagtgct cagcaggtct gtgcccactt
     2521 gcagcgacaa gcgcgatttg cctattccgc cggaaccggt gagggtaacg aggcggtatt
     2581 tagaaagcgc atccttgatc tcggatagtt cttttttgcg accgatgaaa ctggtgagtt
     2641 gggagggcag gtttgtttgt gaaaattggg caggcgccgg ggactctccc aagccgcgcg
     2701 caaagcggac ccatgccggg cgttccaagt ctggaatgcc aagaagtttg agcaatgttt
     2761 ctgcgagttg ctccgatggt ttcaatgtgc cggcttc
//
LOCUS       DAOQNJ010000368         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26654_length_2797_cov_7.548140, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000368 DAOQNJ010000000
VERSION     DAOQNJ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26654_length_2797_cov_7.548140"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLG52_10165"
     CDS             complement(<1..244)
                     /locus_tag="PLG52_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41853.1"
                     /translation="MAKEIWEVGMKKSSLSTQDKTILILIAAILFGTWFRIMPGWLSG
                     FPVNDGGMFYTMIKDLKTNHFFPPLYTSYNHLSIPFA"
     gene            complement(220..2790)
                     /gene="mutS"
                     /locus_tag="PLG52_10170"
     CDS             complement(220..2790)
                     /gene="mutS"
                     /locus_tag="PLG52_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNB41854.1"
                     /translation="MSNEDVTPVRRQYLEAKKQYPDTIVFFRLGDFYETFDEDAEITA
                     RELDIVLTSRPIGGGIRVPLAGIPYHAVENYLSRLIEKGYHVAICEQIGEVPAKGLVE
                     RKVVRVVTPGTVTEPGLLPGDANNYLASVVMEASASSSGTVASVAYTDVTTGEFAVTE
                     LPLEALRAELTRLHPAEILYPDNQKLPDGIIGHTTHVPAWKFEPGKCNEVLLTQFKAS
                     TLDGFGLKANSLSVRAAGAIIQYLKETQPDALHLLNSLRSYSLNEFMTLDASTRRNLE
                     LDETLRGERKGSLLGTLDATITAMGKRMIHQWVSQPLLNVERINKRQNGVEYFVQNGM
                     VRAELREALRPIADLERLINRVIAGHAQPRDLVAMRSTFANLPNILNALKEKGNKKEA
                     QLPTIDLLGEQLSILQNAIDEDPPATLQNTGVIRPGYSTELDGVIDASKHAREWIANL
                     ESVEREKTGIKTLKVGYNKVFGYYIEISRGAADKAPEHYIRKQTLVNAERFITPEMKE
                     YETLVLNAEERIKEIETRLFKEVCGDLSKTANKILATARSIAELDVLSALGEVAALNG
                     YTKPQVHEGSELEITEGRHPVVEQLLKSDRYIPNDVIFEKGEIVRVITGPNMSGKSTY
                     LRQTALIVLMAQMGSFVPAGHAKIGLVDRIFTRIGAQDEIHAGQSTFMVEMVETANIL
                     HHATNRSLLILDEIGRGTSTYDGLSIAWGIIEYVHNHPQLRAKTLFATHYHELTQLAE
                     LLPGVRNYNVAVSEADGKVVFLHKIIAGGADKSYGIHVAQLAGLPAPVIGRANEIMTE
                     LERSAARGVNVNPNAAQQVALFPETNPLLDELKGIDVNSLSPIEALNKLFEWQKRFGK
                     "
BASE COUNT          682 a          643 c          705 g          767 t
ORIGIN      
        1 aagcaaatgg aatacttaaa tgattgtaac tagtgtaaag cggaggaaaa aagtggttgg
       61 tttttagatc ttttatcatg gtgtagaaca ttccaccatc gtttacaggg aaaccactca
      121 gccatccagg cataatacgg aaccaagttc caaataggat ggcagcaatt aagattaaga
      181 tagttttatc ttgagtgctg agagagcttt ttttcatacc tacttcccaa atctcttttg
      241 ccactcgaat aacttattta gtgcctcaat aggtgacagg gagttgacgt caattccctt
      301 caactcatcc agcagtggat tggtctctgg gaagagtgca acctgttgag ctgcattagg
      361 gttgacattc acgccacgtg cagcggaccg ttcgagttct gtcataattt cattggcacg
      421 accgatcaca ggtgcgggaa gacctgcaag ttgagcaacg tgaataccat acgatttatc
      481 tgcgccaccg gcaatgattt tgtgtaggaa aacaactttc ccatcagctt cactaaccgc
      541 aacgttgtag ttacgcacac ccggtaataa ctctgccagt tgtgttaatt catgatagtg
      601 ggttgcaaac aaagtcttgg ctcgcagttg cggatgatta tgaacgtatt cgatgattcc
      661 ccatgcaata gacagaccat cataagtcga agtgccgcgc ccgatttcat ctaaaatcag
      721 caaactgcga tttgtggcat gatgcaaaat atttgcggtt tcaaccattt caaccataaa
      781 ggttgattgc ccagcgtgaa tttcatcttg tgcgccaatg cgggtgaaga tacggtctac
      841 taaaccaatc tttgcgtgcc ctgccggaac aaacgaaccc atttgcgcca tcaacacaat
      901 cagagcggtc tgacgtaaat atgtagactt accactcata ttcggtcccg tgatcacccg
      961 cacaatctcg cctttttcaa agatgacgtc attgggaata taacggtcag actttaataa
     1021 ttgctcaaca actgggtgtc tgccctctgt gatttccagc tcgcttcctt catggacttg
     1081 gggcttggta tacccattca aggctgcgac ttcgcctaga gcagacagta catccagctc
     1141 ggctatagag cgggcggtgg caagaatttt atttgcagtt tttgagagat cgccgcaaac
     1201 ttctttgaat aaacgagttt caatttcttt gatgcgttct tcggcattca agaccagcgt
     1261 ttcatattct ttcatctcag gtgtgatgaa acgctcggca ttgaccaatg tctgcttgcg
     1321 gatgtaatgt tctggggctt tatcggcggc gccacgcgaa atttcgatgt aatagccaaa
     1381 gactttgttg tagccaactt tgagggtctt gatacctgtt ttttcgcgtt cgacggactc
     1441 taggttggca atccattcac gggcgtgttt ggaggcgtca ataacaccgt caagttcagt
     1501 tgagtagccg ggacgaatca caccggtgtt ttgtagtgtg gctggcgggt cttcatcaat
     1561 ggcattttgt aaaatggata attgttcgcc cagcaaatca attgtgggta attgcgcttc
     1621 tttcttgttt cccttttctt ttagggcatt gaggatattt ggtaaattcg caaacgttga
     1681 cctcattgcc acaaggtcgc gcggctgtgc gtgcccagca atcacacgat tgatgagacg
     1741 ctctaaatca gcgatggggc gtaatgcctc gcgcaattca gcgcggacca taccattctg
     1801 cacaaaatat tccacgccat tttggcgttt gttaattcgt tctacattta gtaaaggctg
     1861 gctgacccac tgatggatca tgcgcttgcc catggctgtg atggttgcat cgagtgttcc
     1921 gagcagggaa cctttgcgtt cgcctctcag ggtttcatcg agttcaaggt ttcggcgtgt
     1981 ggatgcatcc aaggtcatga actcgtttag gctataagag cggagcgagt tcagcagatg
     2041 gagagcgtct ggctgcgttt ctttcaggta ctgaatgatt gctccagcag cgcggacgga
     2101 gaggctattg gctttcagtc caaacccatc taaggtagag gctttgaatt gagtcagaag
     2161 tacttcattg catttacccg gttcgaactt ccaagcaggg acgtgcgtgg tatgaccgat
     2221 gattccatcg ggaagttttt gattgtcagg ataaagaatt tcagcggggt gcaggcgtgt
     2281 caactctgcg cgtaaggctt cgagcggaag ttcggtgacg gcgaattctc cagtggtgac
     2341 gtcggtataa gcaacagagg caactgtccc ggaggatgag gcggaagctt ccatcaccac
     2401 agaggctaag taattattcg cgtcgccagg caagagtcca ggttcagtca ccgtccctgg
     2461 ggtgaccaca cgcacaactt tgcgctccac caatccctta gccggtactt cgccgatctg
     2521 ttcgcagatg gcaacgtgat agcctttctc gatcaggcgg gaaagataat tttcaacggc
     2581 gtgatatggg atgcccgcca acggcacgcg gatgccgccg ccgatcgggc gtgaggtgag
     2641 gacgatatcc aactcgcggg cagtgatttc ggcatcttcg tcgaaggttt cgtaaaaatc
     2701 gcctagccga aaaaagacga tggtatcggg gtattgtttt ttggcttcaa ggtattgcct
     2761 gcggacaggc gtgacatctt cgttggacat tccctga
//
LOCUS       DAOQNJ010000369         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26691_length_2795_cov_7.485036, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000369 DAOQNJ010000000
VERSION     DAOQNJ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26691_length_2795_cov_7.485036"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /gene="jag"
                     /locus_tag="PLG52_10175"
     CDS             complement(<1..787)
                     /gene="jag"
                     /locus_tag="PLG52_10175"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNB41855.1"
                     /translation="MSERTTLEIIAPTVEEAIQQGLDQLGLTADAVSVEVLDSGSKGL
                     FGLGGRQVRVRISVNPPPMPEIPAARPEPKPAPAKKAESKPKAKEPKEPKPRAEKKQA
                     EPKTEAKPEPKPEPKPQKPAPQRSTINVENDAVLNTAEEVVSKLIYHMGMTAQVSAHY
                     DESDSADNRTIQVDVRGEDLSALIGRRAETLASFQHVASLIVGKQTQQWVQVIIDVEG
                     YRARREKQIRQLANRMADQVTKTGRKVTMEPMPSSERRVVHIEL"
     gene            complement(797..1615)
                     /locus_tag="PLG52_10180"
     CDS             complement(797..1615)
                     /locus_tag="PLG52_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNB41856.1"
                     /translation="MWESFIIQPMVNLLLWIYDILGHGPQMFGLAIILFTIVIRLITW
                     PLNASQVKGAQAMQELNSDKEWQDIQKKYAKDREKLAQEQMRIYKERGINPFASCLPT
                     LIQFPIIIGLYQSIIRAMAATPLDMLNLARTIYPFQHVSNIIPLNSTFLWMNLGRPEG
                     IPLPFDVSFLPNGFPLLALVVAITTYVQTKLTMPTTPPNPNDQASAQTAAMSSSMSIY
                     MPLMLGWFALTFASGISIYFITSNTLGIIQYAVTGRANWSNLLPGGNKSTPTKK"
     gene            complement(1628..2701)
                     /locus_tag="PLG52_10185"
     CDS             complement(1628..2701)
                     /locus_tag="PLG52_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB41857.1"
                     /translation="MKKINLILLLVLGAVLLSACTGGRASIVNTWAGLAADGERAYLS
                     SGSHVYAVDVQTGKEVWRYPAETDSNIIFYANPVLTNDGQLLVGSEGNNHELVSINPE
                     TGKDNWAKPFSVAKGKWVASPLVFNDIVYAPNSDGFLYILDLNGETSADPIELGGSLW
                     STPVTDGTYIYVTSLDHHLHIVDPASNSIVSTIDLGGASPSSPVVGEGGVYVGSFASN
                     IEFVTSNGDHEVLATATNWIWGTPALDNGTLYYADLDGVLYSLDVNSGKQNWTDVKPD
                     GPVIANPVVVGDAIYFVTEAGTFLALDRDAKTIWEKEVGGKIYTTPVVSGDMVLVAPY
                     KAEFALAAYDAEGKQAWTFTPEK"
BASE COUNT          669 a          676 c          759 g          691 t
ORIGIN      
        1 gcagttcgat atgaaccaca cggcgctcgc tggaaggcat cggttccatg gtcactttgc
       61 gtccggtctt ggtcacctgg tcagccatgc ggttggcaag ctggcgaatc tgcttttcac
      121 ggcgagcgcg gtagccttcc acgtcgatga tgacctgtac ccattgctgg gtctgcttgc
      181 caacgatcaa cgatgcgacg tgttggaagg aagccaaggt ttcggcgcgg cgtccgatca
      241 gggcgctaag gtcttcacca cggacgtcca cttgaatggt gcggttatcg gcagagtctg
      301 attcatcgta atgagcagag acctgtgcag tcatgcccat gtgatagatc agcttggata
      361 ccacttcttc cgccgtattc aacaccgcgt cattctcaac attaatcgtt gagcgttgtg
      421 gagcaggctt ttgcggcttc ggttcaggtt tcggttcagg cttggcttct gttttgggtt
      481 ctgcctgttt cttttcagct cgcggcttgg gctcttttgg ctctttcgct ttcggcttgg
      541 actcagcttt tttcgctgga gcaggcttcg gctcaggtcg agccgcgggg atttccggca
      601 tgggcggagg gttcacagaa atgcgaacac gcacttgacg tccaccgagt ccaaataatc
      661 ctttggagcc ggagtctaga acttctacag aaaccgcatc cgccgtgagc ccgagttggt
      721 cgagcccttg ctggatggct tcttcaacag ttggagcgat aatttctaac gtagtccgct
      781 cactcatggt gcctccctat ttctttgtag gtgtagactt gtttccaccg ggcaacaaat
      841 tgctccagtt ggctcgaccc gttaccgcat actgaatgat gcccaacgta ttgctggtaa
      901 taaagtagat cgaaatgcct gaggcaaatg taagcgcaaa ccaacccaac atgagcggca
      961 tataaatgga catgctgctg ctcatggcag cagtttgtgc agaagcctgg tcattggggt
     1021 tgggcggtgt ggtaggcatg gtcagtttgg tttgaacata ggtagtaatt gccacaacca
     1081 acgccaagag cgggaaaccg ttcgggagga aggacacatc gaacggaaga ggaatccctt
     1141 cggggcgacc gaggttcatc cacaagaatg tactgttaag cgggatgatg ttcgaaacat
     1201 gctggaaagg gtaaatggtg cgcgccaaat tgagcatgtc gagcggggtg gccgccatag
     1261 cgcggatgat gctctgatac aagccgatga tgatcggaaa ttggatcaag gtcggcaaac
     1321 aggaagcgaa aggattaatg ccgcgttctt tgtaaatacg catttgttcc tgcgccagct
     1381 tttcgcggtc tttggcatac ttcttttgaa tgtcttgcca ttccttgtcg ctgtttaatt
     1441 cctgcatggc ttgggcgccc tttacctgtg aggcattgag gggccaagta atgagcctga
     1501 tcacgatggt gaataaaatg atggcaagac caaacatttg cggtccatga cctaaaatgt
     1561 catagatcca caaaagcagg ttaaccatcg gttggatgat aaatgattcc cacatgggct
     1621 aggttcctta tttctcgggg gtaaacgtcc aggcttgttt gccttcagcg tcataagccg
     1681 ctaatgcaaa ttctgcctta tacggcgcaa cgaggaccat gtcgccggaa acaacaggag
     1741 tggtatagat tttgccgccc acttcttttt cccaaatggt ctttgcgtca cgatccaaag
     1801 cgaggaaagt acccgcctca gttacgaaat aaatggcatc gccaacgact actgggttcg
     1861 ctatgacagg accatctggc ttgacatcgg tccagttctg tttgccagaa ttcacatcga
     1921 gggagtaaag aacaccgtcg aggtcggcat agtacaaagt gccgttatcg agtgcgggag
     1981 ttccccagat ccaattcgta gcagttgcca aaacttcatg gtcgccattg gaagtgacaa
     2041 actcaatgtt cgaagcaaat gagcctacat atactccacc ctcacccaca acggggctgc
     2101 tgggagaagc accgccaagg tcaatggtgc tgacgatgga attgcttgca ggatccacaa
     2161 tgtgaaggtg atgatctaac gaagtgacgt aaatgtaagt accatctgtc acgggcgtgg
     2221 accaaagtga gccgcccaat tcgatggggt cggcagaagt ctcaccgttc aagtcgagga
     2281 tgtagagaaa gccgtcggaa ttgggagcgt agacgatatc gttgaaaaca agcggagatg
     2341 ccacccactt ccctttagca acagagaagg gttttgccca gttatctttg ccagtttcgg
     2401 ggttgatgct caccagttcg tgattgtttc cttcacttcc caccaacaat tgaccatcgt
     2461 tggtaaggac tggatttgca taaaagatga tattgctatc ggtttctgcg gggtaacgcc
     2521 aaacttcttt gccggtctgc acgtccacag catagacgtg ggagccagaa gaaagataag
     2581 cacgttcgcc gtcggcggca agacccgccc atgtgttaac aatggaggcg cgtccgccgg
     2641 tgcaggcgct caacaaaaca gcacccagca cgagcaagag tataagattt atctttttca
     2701 tatttaaatt ggatttactc ctaaaaacgg caaaggcagg acctttgccg tgaaagatgc
     2761 actattacgg gacggggtcg tacccgccgg ggtta
//
LOCUS       DAOQNJ010000370         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26777_length_2790_cov_6.139305, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000370 DAOQNJ010000000
VERSION     DAOQNJ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26777_length_2790_cov_6.139305"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLG52_10190"
     CDS             complement(<1..500)
                     /locus_tag="PLG52_10190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB41858.1"
                     /translation="MLQIGDQFDHYQIQAHMAQGGMSDVYRAFDLMHRREVVIKVPDH
                     SMIGDPAQFERFQRELEVMKSLSHPAILKGLDSGKHNRVPYLVTEFVQGESLRTLMEK
                     SGSLPTDQVVSLIQKLANGMAYCHKNEVIHRDLKPENILITSDGQPVIMDFGLALTKD
                     SHRVTYS"
     gene            complement(527..1357)
                     /locus_tag="PLG52_10195"
     CDS             complement(527..1357)
                     /locus_tag="PLG52_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNB41859.1"
                     /translation="MRKFIPEIESSGLSIIGPVREDNQDTIHITDYPNSNGAGFLFAI
                     ADGMGGYAHGGMASALALESFLNVILTHHRQPVLRTLQRGVETANLHVYQKARQMDAG
                     RMGTTLTAAYILGDMLYLTHVGDSRAYLVRDGQATCLTSDHTTVGDMVRAKLIPAAKI
                     RTHAQRSILTKSIGTDLFVQPDILQQKLKPGDCLILCSDGLWSVVEDDDFARVASQSS
                     SVSEVSQNLVDLALAHHTDDNVSVVTFSVRSFMPVSAEQEPLPEESLGWFKKLRKMVS
                     "
     gene            complement(1491..>2790)
                     /locus_tag="PLG52_10200"
     CDS             complement(1491..>2790)
                     /locus_tag="PLG52_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNB41860.1"
                     /translation="LLALLSAALTALSFGELARKYPEAGFRSAYHFADRFFARHKNSK
                     HKSVARLGKFAIGWAAHLYYWIYPGVLIAFMGILADYLLRYFGYQPTVFGDALLAASF
                     AAFAGFLALRGITGTTTTSVILNTIQLLTLLTFGILAITFRLINPAAFAPTEWTYPSA
                     LAVLIPHSFDGLIFQMILAMILMVGFESSTALSAAATNAQRDIPRATILALIIQGLFS
                     YLFGYFIAGFALNAHIDATASQAPMGDIALQIGDLLLDGYGATLMVFFGFTVAIALLG
                     GTLTAINNGVRISFAMALDEEMPDIFGMLHPRYTTPYNTVILLSIVCAIIGSAGIIGG
                     MPVLMGIVLASNLGAFLLYALLCLLTVVTFNGDPAFSFLRHLLVPVIGFLVNVGTVIL
                     AAKIGFSAGGLISQASWVAWGLAGVWFIVSIIYYFLRRKA"
BASE COUNT          779 a          608 c          727 g          676 t
ORIGIN      
        1 gagtaggtga cacggtgcga gtcttttgtc aacgcgagac caaaatccat gatgacgggt
       61 tgtccatcac tagtgataag gatattttca ggtttcaggt cacgatgaat gacttcgttc
      121 ttgtggcagt atgccatccc gttggctaat ttttgaatga gagaaacaac ttgatcggtc
      181 ggtaaagagc cgcttttttc catcaacgtg cgcaatgatt cgccttggac gaattctgtc
      241 acaagatatg ggacgcggtt gtgcttccca gaatccagcc cttttagaat cgctgggtgg
      301 ctaagtgact tcataacttc aagctcacgt tggaagcgtt caaattgggc agggtcgccg
      361 atcatggaat gatcgggaac tttaataaca acctcgcggc ggtgcataag atcaaaggcg
      421 cgatatacat cactcattcc gccctgagcc atgtgtgctt gaatttgata atgatcaaat
      481 tgatcgccga tttgaagcat agtgttcgtc attatagcat aggcattcat gacaccattt
      541 tccttagctt cttaaaccac ccaagtgatt cttcaggtaa tggttcttgc tcggcagaaa
      601 caggcatgaa gctgcgaact gaaaaggtaa ccacagatac attgtcatcg gtgtgatgcg
      661 ccagtgctag atcgactaga ttttggctca cctcagaaac agatgaggat tgggatgcga
      721 ctcttgcgaa atcatcatct tccacaactg accataatcc atctgagcat aggatgaggc
      781 agtcacctgg tttgagcttt tgctggagga tatcaggctg tacgaataaa tcggttccta
      841 tggatttggt gaggatggag cgctgagcat gggttctgat cttggcggcg ggaatcaatt
      901 ttgcgcggac catgtctcct acggtggtgt ggtctgatgt caggcaagtg gcttgaccgt
      961 cgcgaacgag ataggcacgg ctatctccta catgtgtgag gtacagcata tctcccaata
     1021 tgtaggcggc agtgagtgtg gtgcccatgc gccctgcatc catttgacgg gctttttgat
     1081 agacatgcaa gttggcggtt tcaactccgc gctgtaaggt gcgtaaaaca ggctgtctat
     1141 gatgtgtgag aattacgttt agaaaggact caagagctaa tgcgcttgcc atgcccccat
     1201 gagcatagcc acccatacca tctgcgatgg caaagagaaa cccagcccca ttggagttcg
     1261 gataatcagt gatatgaatc gtatcctgat tatcttctcg tacgggacct ataatgctca
     1321 agccgctact ttcgatctcg gggatgaatt ttctcaaaac cagtcgcttt cacagtagtc
     1381 cactcacagc aggcaaaatc aatccctgct gtgaggaaat aaagattaag caattctatg
     1441 taagctggtt gtttgaaaat agtacccaaa tggggcattt cagcttgtgg ttaggctttt
     1501 cgcctcaaga agtagtagat gatactaact ataaaccaca cgccagctag cccccaagca
     1561 acccaacttg cttgactaat gagtccaccg gcagagaaac caatcttggc tgccaagatc
     1621 acagtgccaa cattgactaa gaacccgatc accgggacga gaagatggcg cagaaaactg
     1681 aatgctgggt caccgttgaa tgtgacaacg gttaacagac agagcagagc ataaagtaga
     1741 aacgcgccga gattggatgc cagaacgatg cccattaata cgggcatgcc gccaatgatt
     1801 ccggcagagc cgattatggc gcagacaatg ctgagtaata taacggtgtt ataaggcgtg
     1861 gtgtatcgag gatgtaacat gccaaaaata tctggcatct cttcatccag cgccatggcg
     1921 aagctgattc gtacgccgtt gttgatggcg gttaatgtcc caccgaggag ggcgatggcg
     1981 acggtaaagc cgaaaaatac cattaaggtg gcaccataac cgtctaataa caagtcgccg
     2041 atttgtaggg caatatcgcc catgggtgct tgtgatgcag tggcatcaat atgggcgttt
     2101 agtgcgaacc ctgcaatgaa ataaccaaaa agatatgaga ataagccttg aatgattaac
     2161 gccagaattg tcgcgcgcgg aatgtcgcgt tgggcattgg tagccgcagc gctcaatgcg
     2221 gtagacgatt caaacccgac cattaggatc attgctagaa tcatttggaa gatcagcccg
     2281 tcaaagctgt ggggtatgag aacggctaag gcggagggat aggtccactc tgtgggggca
     2341 aatgcggcgg ggttgatgag gcggaatgta atcgcaagaa tgccgaatgt gagtagagtc
     2401 aataactgaa tggtgttcaa gatcacgctg gtggtggtgg tgcctgtaat tccgcgtaat
     2461 gcgaggaaac cagcaaaggc agcgaacgaa gcggctaaga gtgcatctcc gaatacagta
     2521 ggttggtacc caaagtaacg tagtaaataa tcggcaagaa tgcccatgaa ggcaatcaga
     2581 acgccggggt agatccaata ataaagatgt gcagcccaac caattgcgaa ttttcccaaa
     2641 cgtgctacag acttatgttt ggaattttta tgacgcgcaa aaaaacggtc tgcaaaatgg
     2701 taagcgcttc gaaaccccgc ttcaggatat tttctagcaa gctctccaaa tgaaagggct
     2761 gtgagtgctg ctgaaagtaa tgccaataaa
//
LOCUS       DAOQNJ010000371         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26837_length_2786_cov_8.132552, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000371 DAOQNJ010000000
VERSION     DAOQNJ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26837_length_2786_cov_8.132552"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLG52_10205"
     CDS             <1..545
                     /locus_tag="PLG52_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41861.1"
                     /translation="ISNAAFEAHRAGKVRVTSARGSDFFGPWGLPTAAMGERTFYPMI
                     AGKAAGLVGNVDVPHTHTYIPNFGKALVILGERDEADGQAWHVPNDNPRVTQREMAQM
                     IADELGLPLKFSAMGKTMMAIGGLFIPEAKESVEMMYEFEKPFIVDSSKFEKTFGMKA
                     TPMKEAIKETVAWYKSHPHK"
     gene            571..1023
                     /locus_tag="PLG52_10210"
     CDS             571..1023
                     /locus_tag="PLG52_10210"
                     /inference="COORDINATES: protein motif:HMM:NF021495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HNB41862.1"
                     /translation="MVFVNSIMASLHHIAAFTVAACLVYEFVAYRKNLTVAEARNIQR
                     VDLAYGIAAGVVLVFGFLRVYFFEKGSAYYFSNHVFWTKIVLFVIIGLLSIYPTIRYI
                     KWNPVLAENKVPEIPEKEYRNIRLLLWLEMAGLVLILFAAPLMARGIG"
     gene            1097..2011
                     /locus_tag="PLG52_10215"
     CDS             1097..2011
                     /locus_tag="PLG52_10215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HNB41863.1"
                     /translation="MLEIAVMLEGQNGLNWTHWQNIVRLAEDLGFVGLYRSDHFTNMN
                     PPDKDSLELWVSLTWLASNTKRIEFGPLVTPVSFRHPALTARMAAAVDDLSNGRLTLG
                     LGAGWQEREHHLFGFDLLDTKPCFERFEEGLHVITGLLNSETPLSFDGTFYQLREATL
                     LPRPLRPGGPRILVGGNGQKTTPRLVSQFAHEWNAVFLSLEQFKEANQRIDDALTDAN
                     RDLHSVRRSMMTGCIFGHTEASLREKIEARGRTKEELQQRGIIVGSANEVQEQLHRLQ
                     EAGLQRIMLQWLDLDDLAGLEALAKAVL"
     gene            complement(2020..>2786)
                     /locus_tag="PLG52_10220"
     CDS             complement(2020..>2786)
                     /locus_tag="PLG52_10220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB41864.1"
                     /translation="YAWHMRDDYLKLHGLTNPLIQFAATSTLGLLRRWDTFASARSDS
                     LIANSYWTASHIQHAWQRKADVIYPPVDVQRFVPAQQRDDYYLLVSRLVPYKMGIEIT
                     KAFNSLGLPLVIIGDGPELQHVRSIAKENITIMGYQPDAIVTEFMNKAKAFVYMATED
                     FGIAMVEAQAAGCPVIAYCKGGASEIVRDGETGLLFKEQTSEALADAVLKFESMNLNS
                     KAASDNAARFSAERFRREFLDHIQKIASSGKAPSSQ"
BASE COUNT          669 a          627 c          746 g          744 t
ORIGIN      
        1 agatcagcaa tgcggcgttt gaagcgcatc gtgccggaaa agtacgtgtc acctcggcgc
       61 gcggctctga ctttttcggt ccgtggggtt tgccgacggc tgccatgggc gagcgaacgt
      121 tttacccgat gatcgcaggc aaggcggcag gcttggttgg caatgttgac gttccgcata
      181 cacataccta tatccctaat tttggaaaag cacttgtcat tcttggcgaa cgcgatgaag
      241 ccgatgggca agcttggcat gtgccgaacg ataatccgcg tgtgacccag cgtgaaatgg
      301 cgcagatgat cgccgacgag ttgggacttc cgctcaagtt tagtgcaatg ggtaagacta
      361 tgatggcgat tggtggtttg ttcatccctg aggcaaaaga gtctgtagag atgatgtatg
      421 agtttgagaa gcctttcatt gtagattcga gcaagttcga gaagactttt ggcatgaaag
      481 ctacgccgat gaaagaagcg atcaaagaaa cagtggcttg gtataagagc cacccacata
      541 agtaacaaaa acttttagag gagatgtttt atggttttcg taaattcgat tatggcaagt
      601 ttgcatcata tcgcggcatt caccgtagcg gcatgtttgg tgtatgaatt tgtcgcgtat
      661 cgcaaaaact tgacggttgc cgaggctcgc aatattcagc gggttgatct ggcgtatggg
      721 attgcagcag gcgtggtttt ggtctttggc tttttacgag tgtatttttt cgaaaaaggc
      781 tctgcctatt atttttccaa tcatgtcttt tggacaaaga ttgtactgtt cgtgattatt
      841 ggtttgttat ccatttatcc gaccattcgt tatataaaat ggaatccggt attggcagag
      901 aacaaagtgc cggagatccc ggagaaagaa tacaggaata ttcgcctatt gctttggctg
      961 gaaatggcag ggctggtgtt gatcttgttt gcagcgcctt tgatggcacg tgggatcgga
     1021 taggctcaga cctcggaggt ttttaaaacc tccgaggtct ttttttgaag tagactcagc
     1081 ttgaaaggag ttctacatgc ttgagatcgc tgtaatgctc gaaggtcaga atggattgaa
     1141 ctggacgcac tggcaaaata ttgttcggct tgccgaggat ttgggttttg ttgggcttta
     1201 tcgctccgac cactttacca atatgaaccc acccgataaa gactcgctgg agttgtgggt
     1261 ctcgctcacg tggctggctt ccaacaccaa gcgcatcgag ttcggtccgc tggtgacgcc
     1321 ggtttcgttc cgtcaccccg cgcttacggc tcgcatggca gctgcggtgg atgacctctc
     1381 aaacggacgt ctcacacttg gtctgggcgc tggctggcag gaacgtgaac atcatctctt
     1441 tggcttcgat cttttggata ccaagccgtg cttcgaacgt tttgaagaag gtctacatgt
     1501 cattactgga ctcttgaata gtgaaacgcc tctttccttc gacgggacgt tttatcagct
     1561 ccgtgaagcg actctactcc cacgtccgct tcgccctggc ggacctcgca tcttggtcgg
     1621 cggaaacggg cagaagacaa cgccgaggct tgtctctcaa ttcgctcacg agtggaatgc
     1681 tgtttttctg tcactggagc agttcaaaga agccaatcaa cgcatcgatg atgctttgac
     1741 cgatgcaaac cgtgatctac attctgtgag acgatctatg atgacaggct gtatcttcgg
     1801 gcataccgaa gcttctctac gagaaaagat cgaggcacgc ggacgtacca aggaagagtt
     1861 gcaacagcgc ggaattattg ttggttcagc aaatgaagtg caggaacaac ttcatcggtt
     1921 acaagaagct ggcttacagc gcatcatgct ccaatggctg gatttagatg atcttgccgg
     1981 gctggaagct ctggcgaaag cagtgttata aggagtatgt cattgcgagg agggtgcttt
     2041 tcccgacgaa gcaatctttt gaatatgatc taaaaattcc cgcctgaatc tttcagcaga
     2101 aaagcgggcc gcattgtctg atgcggcttt gctgtttaag ttcattgatt caaatttcaa
     2161 gactgcatcc gctaaggctt cggatgtttg ttctttgaac aaaagccctg tctctccatc
     2221 tcgcacgatc tccgatgcgc cgcccttgca atatgcaatg acaggacatc ccgccgcctg
     2281 cgcttcgacc atggcaatcc caaaatcctc ggttgccatg tacacaaagg ctttggcttt
     2341 gttcatgaat tcagtgacga ttgcatcggg ttgatacccc atgatcgtga tattttcttt
     2401 ggcaatactg cgaacgtgtt gaagttcggg tccatcaccg atgatgacaa gcggtaaacc
     2461 gagagaatta aatgccttgg tgatctcaat ccccattttg tacggaacca ggcgtgaaac
     2521 gagaagataa tagtcatcgc gctgctgagc aggtacaaac cgttgtacat ccacaggcgg
     2581 ataaataaca tctgctttgc gctgccaggc atgctgaata tgggacgccg tccaatatga
     2641 gttggcgatc agcgaatcgg atcgggcgga tgcgaaggtg tcccaacggc gcagcaagcc
     2701 cagggttgag gttgcggcga actgtataag tggattggtc agaccgtgca attttaaata
     2761 gtcatcacgc atatgccagg catagc
//
LOCUS       DAOQNJ010000372         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26869_length_2784_cov_7.046537, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000372 DAOQNJ010000000
VERSION     DAOQNJ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26869_length_2784_cov_7.046537"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLG52_10225"
     CDS             <1..531
                     /locus_tag="PLG52_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41865.1"
                     /translation="DEMVDKFQDPNGGFFDTPIDGENLLFRPKDVQDNAIPSGNSLAC
                     EALLKLAAYTDNGNYRDIAEKSFGLVHKFVLQYPLGFGRWLTAAQLATGTLKQVAAVG
                     EAGDPNFQSLLKVIWSEYRPNVVVAASSSLEKNTPALLHDRPLMHGKATVYVCEGFVC
                     QQPTTEADVLAEQLKN"
     gene            complement(567..746)
                     /locus_tag="PLG52_10230"
     CDS             complement(567..746)
                     /locus_tag="PLG52_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNB41866.1"
                     /translation="MGLPRGSEWIFILIIVVLVFGPGRIGKVAGEIGKSIKSFRDGLS
                     GEEEAEKKSDTTEQK"
     gene            complement(805..1908)
                     /locus_tag="PLG52_10235"
     CDS             complement(805..1908)
                     /locus_tag="PLG52_10235"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNB41867.1"
                     /translation="MPYAHITGWGVSVPEPVLTNDDLAKMVETNDEWIRERTGIRERH
                     IAREDQSTATLAVDAAIKALQVANLAPTELDLIIVTSSSPEYIFPATACLVQDQIGAT
                     KAGAFDLLAACSGFIYATNMAAQSIRSGSIKNALVIGSETLSRFVNWKDRSTCILFGD
                     GAGAFVIQASDQPGGVLSAVMHSDGSGADSLTLLGGGARFPANETTVAEGKHYVQMDG
                     KAVFRFATRVMGTATKEALELANVKLEEVDYIIPHQANYRIIETAAKYLKLPLDKFII
                     NVDRYGNTSTASIPIAAVEAIEKGKLKSGNKVVFVGFGAGLTWGALVAQWSGPIPAKK
                     QVHPEQYRLFARLRSWLLRALRFLEGLLSRREL"
     gene            complement(1918..>2784)
                     /locus_tag="PLG52_10240"
     CDS             complement(1918..>2784)
                     /locus_tag="PLG52_10240"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNB41868.1"
                     /translation="GPERVSPFLIPMMIADSAAGNLAIRIGARGPNMSLATACASGTN
                     GVGEAAEMIRRGAADVMVAGASEAAIHSLTMAGMNVMTALSTRNDDPQRASRPFDKDR
                     DGFLMGEGSAVLILESLDHALARGANILCEFTGYGTSDDAHHISAPAENGAGAAISMS
                     LALKDAGFGVADIQYINAHGTSTQLNDKSETAAIKTVFGEQAYKLAVSSTKSMTGHLL
                     GASGALEAMISALTIMNQTLPPTINYETPDPNCDLDYVPNQPRKTNVTNVMSNSFGFG
                     GHNATLVLSRYQ"
BASE COUNT          752 a          687 c          639 g          706 t
ORIGIN      
        1 gacgaaatgg tcgataaatt ccaagacccg aacggtggct tctttgatac ccccattgat
       61 ggtgaaaacc tcctgtttcg cccaaaagat gtacaagata acgcaatccc ttcagggaat
      121 tccttagctt gtgaagcctt attaaagtta gccgcatata ctgacaatgg taactatcgt
      181 gatattgcag aaaaatcatt tggcttggtg cataaatttg tcttgcaata tccgcttggg
      241 tttgggcgct ggcttacagc ggcgcagctt gctacaggaa cgctgaaaca ggtagcggcg
      301 gtgggagaag cgggagaccc aaacttccag agtttgctca aagttatttg gtctgaatat
      361 agaccgaacg tggtggtggc tgcatcgtct tctttagaaa aaaacacccc cgcattgctc
      421 catgaccgtc cactgatgca tggtaaagcc actgtgtatg tgtgtgaagg ctttgtttgc
      481 caacagccaa caacagaagc tgatgtgttg gctgagcagc taaaaaatta aacaaaaaac
      541 agaccgtgat tacggtctgt ttttaattac ttctgttctg ttgtatcgga tttcttctct
      601 gcttcttctt cgccgctcaa accatcacgg aaagatttga tgctcttgcc gatctcacca
      661 gctactttgc caatgcgacc cggaccaaaa acaagaacaa cgataatcag aatgaaaatc
      721 cactctgaac cacgtggtaa acccatagta cgctccttga ttgaatttca tatcaatcgt
      781 accatagttc aaatgaaaac ttgattagag ttcgcggcga gagagcaagc cttcaaggaa
      841 acgcagggca cgtaacagcc atgaacgaag gcgcgcaaac aaacgatact gctcggggtg
      901 aacttgtttc tttgcaggga tgggaccgct ccattgtgca acgagcgcac cccaggttaa
      961 acccgcacca aagccaacaa agaccacttt gttaccgctt tttagtttgc ctttttcaat
     1021 cgcttctact gcagcaatgg gaatagatgc tgtggaggtg ttaccgtaac gatcgacatt
     1081 gataataaat ttatctaagg gcagctttag gtattttgcc gctgtttcaa taatgcggta
     1141 gtttgcctga tgcggaataa tatagtccac ttcttcaagt ttcacgttgg caagttcgag
     1201 agcttctttg gttgcagtac ccatcacgcg tgtagcaaat cgaaaaacag ctttgccatc
     1261 catttgcaca taatgcttgc cttctgcaac tgtggtttca ttagctggga agcgtgctcc
     1321 accgcccaaa agcgtcaagg agtctgcgcc agagccgtct gaatgcatga ccgccgataa
     1381 gaccccaccg ggctggtcac ttgcttggat aacgaaggct ccagcgccat cgccaaaaag
     1441 aatacaagtg ctgcggtctt tccagttcac aaaacgagag agggtctcag agccaatgac
     1501 taaggcattt ttgattgaac cacttcgaat cgactgtgcc gccatattgg tggcgtaaat
     1561 aaaacctgaa cacgctgcaa gtaaatcgaa tgctccagct ttagtagcac cgatctgatc
     1621 ctgcaccaga catgcagttg ctgggaaaat atattctggc gaagaagatg taacgatgat
     1681 caaatccaat tcagttggcg ctaaattagc cacttgtaaa gctttgatcg ccgcatcaac
     1741 tgccagcgtc gctgtggatt ggtcttctcg ggcgatgtga cgttcacgaa tcccagtacg
     1801 ctcgcgaatc cattcatcat tggtctcgac catttttgca aggtcgtcat tcgtcaacac
     1861 cggttcaggg acacttacac cccaacccgt aatatgcgcg tatggcatgc tttctcctta
     1921 ttgatagcgg ctaagtacca aagtcgcatt gtgtccacca aaaccgaacg aattagacat
     1981 aacattggtc acattcgtct tgcgcggttg attggggacg taatcgagat cacagttcgg
     2041 atcgggagtt tcataattga tcgtaggcgg caaagtctgg ttcatgatgg tcaaggcaga
     2101 aatcatggct tccaaagcgc cagaagcccc caaaagatgc ccggtcatcg acttggtaga
     2161 agatacggca agtttatagg cttgttcacc aaacacagtc ttgatcgcag cagtttcact
     2221 tttatcattc aactgcgtgg atgtgccatg ggcattgatg tattgaatat ctgctacacc
     2281 aaagccagca tctttcaatg ccaaactcat ggaaatagca gcacctgccc cattttctgc
     2341 tggagcagaa atatgatgcg cgtcatctga agtgccatag ccagtgaatt cacacagaat
     2401 gtttgcccca cgagctaaag cgtggtccaa agactctaag atcaatactg ctgagccttc
     2461 acccatcaag aacccatctc ggtctttgtc aaacggacgg gaagcacgct gcgggtcatc
     2521 attgcgagtt gaaagcgcag tcatcacatt catgcctgcc attgtcaacg aatgaatggc
     2581 agcttcagaa gcgcccgcta ccattacatc tgcagcgcca cggcgaatca tttctgccgc
     2641 ttcccccaca ccattcgtgc ctgaagcaca agcagttgcc aaggacatgt ttggtccacg
     2701 tgcgccaatg cgaatggcaa ggttgcccgc tgcgctatct gcaatcatca ttgggatcaa
     2761 aaatgggctt acacgttctg gtcc
//
LOCUS       DAOQNJ010000373         2780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_26930_length_2780_cov_6.159266, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000373 DAOQNJ010000000
VERSION     DAOQNJ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26930_length_2780_cov_6.159266"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLG52_10245"
     CDS             <1..535
                     /locus_tag="PLG52_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB41869.1"
                     /translation="ASQKSGTIRIQSGFLNMGLGIGARSQGRYEVAQRHFEEGLRIFK
                     HIGHKGMIAAMTSEIAHTQRALGNYAEAKQTYYETIKVFQDYGNRPSVAHQLECFAMI
                     AIVEEEPQRAAKLFGAAGAIREMTGHRPTDEEQAEEAQFITRLHAMLPEAEFNALWAE
                     GKSMTMEQAIEMALSGS"
     gene            complement(536..964)
                     /locus_tag="PLG52_10250"
     CDS             complement(536..964)
                     /locus_tag="PLG52_10250"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB41870.1"
                     /translation="MIQYEVSVHINQPVEKVFAYLADARNLRNWQADLIENESLTEGP
                     IRVGSRFREVRQTGPRKSNIQAEITAFEPNKRFATKTVVGPQVTVQYAFNAEQGGTKI
                     TYQFSLQTSGFMRLMEPLMAGSIKQGAEADFQKLKQILES"
     gene            1040..1687
                     /locus_tag="PLG52_10255"
     CDS             1040..1687
                     /locus_tag="PLG52_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNB41871.1"
                     /translation="MKKTSPTKRKYDSSRRQTGAAETRQHIMDATLKLFVERGYAGAT
                     IDAIAKEAGVALKTVYAVFENKRNILVALLSRSSSSTGEENIPMLERSGPKAVAQERD
                     QRRQIQMFAQVIAGNLEGAAYIAEIIHIAAKTEADVATLEKKLNEQRWKNMAYAVKVF
                     SANGTLRVKDEASATETVYTLTSPEVFLLLTRERGLSKEEYIDWLADILTRALLP"
     gene            1761..2459
                     /locus_tag="PLG52_10260"
     CDS             1761..2459
                     /locus_tag="PLG52_10260"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNB41872.1"
                     /translation="MKDNSLYKLGGFASIIVGISYVVIGITTVLLPPNLAGVPEAQSP
                     FMFFEANRGLLMTQYWAFTIGAIFALAMIPAVSATVQHLNEGWVRWTSSLATLGFVAA
                     ILDNYWAIVVTPARAAMYTTGSEVVRAALSIPGEPQVLDVQGWLASGGVGLWVLVIGL
                     LALRGKVFPKGLAYLWIIGAFTYFMSLAASVIPDLYLSGVVIAVSSIGAVLAPVGYGW
                     MGWWLWNKAQEIAP"
     gene            2524..>2780
                     /locus_tag="PLG52_10265"
     CDS             2524..>2780
                     /locus_tag="PLG52_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41873.1"
                     /translation="MDITIRRLEPNDYEAMQKIFAGPKVIWGTLQLPYPSIEIWRNRI
                     AEVPNDMFRLAAMVGAEMAGQLDMFTSPNHPRRRHTGQIGMA"
BASE COUNT          694 a          679 c          736 g          671 t
ORIGIN      
        1 agctagccag aaaagcggaa ccatacggat tcaatctggt tttctcaata tgggtttggg
       61 gatcggtgcg cggtctcagg gtcgctatga agtcgcccag cgtcactttg aggaagggct
      121 caggatattc aagcacattg gtcacaaagg aatgatcgcg gcgatgacat ccgagattgc
      181 gcacacacaa cgcgccctag gcaattacgc cgaagccaag caaacctatt acgaaaccat
      241 caaggttttt caagattatg ggaatcgtcc gtccgtggca catcagttgg aatgttttgc
      301 gatgatcgcc atcgtggagg aagaaccgca gcgcgccgca aaattattcg gcgcagcagg
      361 tgcgattcgt gagatgacgg gtcataggcc caccgacgaa gaacaagccg aagaagcgca
      421 gttcattacc cgacttcatg cgatgctccc cgaagcggag ttcaacgcgc tctgggcgga
      481 gggtaagtcc atgacgatgg agcaggcgat tgagatggcg ttatcaggct cttgatcagc
      541 tttcaagaat ttgttttagt ttttggaaat ctgcttccgc gccttgcttg atggagccag
      601 ccatcagcgg ttccatcaag cgcatgaacc cactggtctg cagggaaaat tgatacgtga
      661 tcttggtacc tccctgctcg gcattgaaag catactgaac ggtgacttgt ggtccaacaa
      721 ccgttttggt agcaaagcgc ttgttcggct cgaaagccgt gatctccgcc tgtatgttgg
      781 acttgcgtgg tcctgtctga cggacttctc ggaagcgcga accaacgcga atcggtcctt
      841 cggtcaaaga ctcgttctcg atcaaatccg cctgccagtt gcgcaggttg cgcgcatccg
      901 caagataggc aaacaccttt tcaactggct gattgatatg cacacttacc tcatattgaa
      961 tcatgggatg ctcctttggc gtcgctaaac agtaaattca ttgcactaat ggtataatta
     1021 atttagagaa atgtcaagca tgaaaaagac atcccccacc aaacgcaaat acgactcctc
     1081 ccgtaggcag acaggggcgg cagagactcg ccagcacatt atggatgcca ccctcaaatt
     1141 atttgttgag cgcggatatg caggtgcaac cattgatgcc attgccaaag aagcaggcgt
     1201 tgcgctgaaa acggtctatg cagtatttga gaacaagcgc aacattctcg ttgcgctctt
     1261 gagtcgttca tcttccagca ctggggaaga aaatattccc atgctggaaa gatcaggtcc
     1321 caaagcggtg gcgcaagaaa gagatcaacg caggcagatc cagatgtttg cgcaagtcat
     1381 cgctggtaat ttggaaggcg cggcatatat tgccgagatc attcacatcg ccgccaagac
     1441 agaagcagat gtggcaacgc tggagaaaaa gcttaacgaa cagcgctgga agaacatggc
     1501 gtatgctgtg aaagtgtttt ccgcgaatgg aactctccgc gtcaaagacg aagcttcagc
     1561 cactgaaacg gtatacacct taaccagccc tgaagtgttc ctgcttctaa cacgcgagcg
     1621 cggtttatcg aaagaggaat atattgactg gctggcagac attctaacaa gagcgttgct
     1681 gccataataa acttgatgaa gcgagtgacc aagctagcat tgggttaacg ggaaattttt
     1741 taggagtaaa aggagaacac atgaaagaca attcactgta caaacttggc gggttcgcct
     1801 caatcatagt aggtatttca tatgtcgtta ttgggattac caccgtgctc ctcccgccta
     1861 acctggctgg cgttcccgag gcacagtcac cttttatgtt ttttgaagcc aaccgcggct
     1921 tgctgatgac ccaatattgg gcattcacca tcggagcgat ctttgcactg gcaatgatcc
     1981 ctgcagtatc agcaacagtt caacatctaa acgagggctg ggtgcgctgg acttcgtcac
     2041 tagcaacgct cggctttgtg gcggcaatcc ttgataacta ttgggctatt gtagtaactc
     2101 ccgcccgcgc cgctatgtac acgactggca gcgaggtggt ccgcgccgca ttatcaattc
     2161 ctggtgagcc tcaggtcctt gatgttcagg gctggcttgc gagcggcgga gttggattat
     2221 gggtattggt gatcggtcta ttggcattac gcggcaaggt ctttcccaag gggctagcct
     2281 atttgtggat catcggcgcc tttacgtatt tcatgtcgct tgccgcctca gtcattcctg
     2341 atctttatct ttcaggtgtg gtgatcgctg tctctagtat tggtgctgtt ctggctcccg
     2401 ttggctatgg ctggatgggc tggtggcttt ggaataaagc tcaagaaatc gcgccatgac
     2461 gatggagcag gcgatccagt tggcgttggg ttaaaacacc aaaccagaag atgaattggc
     2521 attatggata tcaccatccg tcgtttggaa ccgaacgatt acgaagccat gcaaaagatc
     2581 tttgcgggtc caaaagttat ctggggtact ttgcaacttc cctacccttc cattgaaatc
     2641 tggcgtaatc gcatcgcgga agtgcccaac gatatgtttc gactcgcagc aatggtagga
     2701 gccgagatgg ctgggcaatt ggatatgttc acgtccccca accatccacg ccgcaggcac
     2761 acggggcaga tcggcatggc
//
LOCUS       DAOQNJ010000374         2768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_27152_length_2768_cov_6.405824, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000374 DAOQNJ010000000
VERSION     DAOQNJ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27152_length_2768_cov_6.405824"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PLG52_10270"
     CDS             complement(<1..799)
                     /locus_tag="PLG52_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB41874.1"
                     /translation="MSEPLVRVENLTKHFPIRKGMLIERVVGAVRAVDNISFTIARGE
                     TLGMVGESGCGKTTAGRTLLGLYPATSGKTIIDGHSVHEARGSELMKVRRKAQMIFQD
                     PYASLNPRWTVSSIISEPLRVHGLLSSQKARDERVKELLELVGLNARHINRFPHEFSG
                     GQRQRIGIARALASDPLFIVCDEPISALDVSIQAQVVNLLEKLQDQLGLTYLFVAHDL
                     SMVRHICDRVSVMYLGVMVELAERDELYENPQHPYTKALLSAVPVPDP"
     gene            complement(796..1791)
                     /locus_tag="PLG52_10275"
     CDS             complement(796..1791)
                     /locus_tag="PLG52_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41875.1"
                     /translation="MKTDHKFLEVKDLKTQFNTPEGTVYAVNGVSFSLNEGETLAVVG
                     ESGCGKSVTMMSIMKLIQMPPGKIVGGEVIFRDSDLLKLSEEAMEQIRGREIAMIFQD
                     PMTSLNPVLTVGRQITETLRTHTPMTPEQCRDRAAQLLELVGIPDPVKRLDDYPHQFS
                     GGMRQRVMIAMALACTPSLLIADEPTTALDVTIQAQIVELVKDLKKSVGMAMIWITHD
                     LGVVAGMADRVLVMYAGYVVEEAPVDDLYENTLHPYTLALLQALPRADRRRDQSLKSI
                     KGSPPNLLMEPKGCPFAPRCEFAQEKCIHENPTLAEFEPNHKVACWVDVKTGALR"
     gene            complement(1798..2748)
                     /locus_tag="PLG52_10280"
     CDS             complement(1798..2748)
                     /locus_tag="PLG52_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB41876.1"
                     /translation="MSTVQASNQSGTMIAEIEAPSLSLRDLTWRRFRRHKMAIVGLGV
                     LILLIIYSFGGAFFVTEKYSNFTNTKERLQPPSAEHPFGTDTVGRDLMARTIYGGQIS
                     IIIGITAAFVEVMMGILIGAIAGYFGGKIDAFLMRFTEAMLNLPQLLLLIVMAKFFSS
                     DIKNINLFGREFSGSVVVIIFIIGLTSWPYLARIVRAEFLSIKEREFILAARATGTPT
                     WEIIFKHILPNSIAPIVVSATLGVAGAISLEAYVSFLGLGVRAPTATWGNMLENAYRY
                     IESAYWMWLFPGLFIVLIVLSINFLGDGLRDALDPRSRTV"
BASE COUNT          766 a          711 c          611 g          680 t
ORIGIN      
        1 gcgggtctgg cacaggaacg gcggaaagca aagcctttgt ataggggtgt tgtggatttt
       61 catatagttc atctcgttca gccaattcca ccatcacacc caaatacatc accgagacac
      121 ggtcacagat atggcgaacc atgctgaggt catgagctac aaagaggtat gtgagaccga
      181 gttgatcttg aagtttctcc agcaaattaa caacttgtgc ctgaatagaa acatccaaag
      241 ccgaaatcgg ctcatcgcag acgatgaaca gcgggtctga agccagggct cgagcgattc
      301 caatgcgctg acgttgtccg ccagagaact cgtgcgggaa gcgattgata tgccgagcgt
      361 tcaaaccgac caattccaaa agctctttta ctcgttcatc tctcgctttt tggcttgaaa
      421 gcaagccatg tactcgcaaa ggctcactga tgatcgaact taccgtccaa cgagggttca
      481 gggatgcgta ggggtcttga aagatcatct gcgctttgcg gcggactttc atcagttcac
      541 taccacgggc ttcatgcaca ctatgcccat caataatggt tttgccagaa gtagcagggt
      601 ataacccaag caaagtacgc ccggcggttg tcttaccgca gccactttcg ccaaccatgc
      661 ccaaggtttc accgcgcgca atggtgaatg aaatattgtc cacagctcgt acagcgccaa
      721 ccacacgttc gatcaacatc ccttttcgaa ttgggaaatg cttggtcagg ttttcaactc
      781 gtaccaatgg ttcgctcatc gcaatgctcc agtctttaca tctacccagc aggcgacttt
      841 atggtttggt tcaaattcag ctaatgtagg gttttcatgg atgcattttt cttgagcgaa
      901 ttcacagcgt ggcgcgaagg gacatccttt aggttccatc aacagatttg gcggagagcc
      961 cttaatagat ttcaagcttt ggtcgcgtcg gcgatctgct cgtggtaaag cttgcagtaa
     1021 cgccaaggta taaggatgca atgtattttc atacaggtca tccacaggag cttcttccac
     1081 tacatacccg gcatacatca caagcacacg atctgccata cctgccacaa cacctaaatc
     1141 atgcgtgatc caaatcatgg ccatgcccac gcttttcttt aggtctttca ccaattcaac
     1201 gatctgcgcc tgaattgtca catccaaagc tgtggtcggt tcatctgcga tcaacaaact
     1261 gggcgtgcaa gccaacgcca tagcaatcat cacacgctgg cgcatgccac cagaaaactg
     1321 atgtggataa tcatccaacc gtttgactgg gtctggaatc ccaacaagtt ctaataactg
     1381 agcagcgcga tctcgacact gttcaggcgt cataggagta tgcgtccgca gcgtttctgt
     1441 gatttgacga cccacggtca acacaggatt tagactggtc attgggtctt ggaagatcat
     1501 cgcaatctca cgcccgcgga tttgttccat cgcctcttca cttaatttga gcaggtcgct
     1561 gtctcgaaag atcacttccc cgccgacaat tttcccgggt ggcatctgaa ttaatttcat
     1621 gatggacatc atcgtcaccg atttgccaca accactttcg cctacaacag ctagtgtctc
     1681 accttcgttc aagctaaacg aaacgccatt cacagcatag acagtccctt ccggtgtatt
     1741 gaactgcgtc ttgaggtctt ttacttcaag gaatttatgg tctgttttca taagtcatta
     1801 caccgttcgg ctacgaggat caagagcatc acgcaagcca tcacccaaaa agttgatgct
     1861 aagcacgatc aaaacaataa ataaaccggg gaacagccac atccaatacg cagactcaat
     1921 gtagcgatag gcgttttcga gcatattacc ccaagtcgct gttggtgcac gaacgcctaa
     1981 gcctaaaaag ctgacatatg cttcaaggga gattgcgccc gcaacgccca aggtagcgga
     2041 tacgacgatt ggagcaatgc tattcggcag aatatgcttg aaaataatct cccaagtagg
     2101 ggttccagtt gcgcgtgccg caagaataaa ctcgcgttct ttgatagaca gaaattcagc
     2161 acggacaata cgcgccaaat atggccaact cgtaagtcca atgatgaaga taattacgac
     2221 cacactgccg ctaaactccc ttccaaagag attaatattt ttaatatcac tggaaaagaa
     2281 ctttgccatt acgatcaaca aaagcaattg cggtaaattc agcatggctt cggtaaagcg
     2341 catcagaaat gcatcgatct tcccaccgaa atagccagca atcgccccaa tgagaattcc
     2401 catcatcact tctacaaaag cggctgtgat cccgataatg atagagatct gcccgccata
     2461 aattgtacga gccataaggt ctcgtccaac ggtgtctgtt ccgaagggat gttccgcgct
     2521 cggcggttgt agtcgttctt tggtattggt aaagtttgaa tatttctctg taacgaaaaa
     2581 tgctccgcca aaagaataaa taatcaaaag aataagaacc cccagcccaa caatcgccat
     2641 tttatgacgg cggaaacgtc gccaagtaag gtctcgcagg gataatgaag gtgcttcgat
     2701 ctcggcgatc atcgttccag actgattgga agcttgaacc gtggacataa ctctctccta
     2761 atcaaagc
//
LOCUS       DAOQNJ010000375         2766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_27183_length_2766_cov_11.417189, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000375 DAOQNJ010000000
VERSION     DAOQNJ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27183_length_2766_cov_11.417189"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PLG52_10285"
     CDS             complement(<1..1048)
                     /locus_tag="PLG52_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41877.1"
                     /translation="MSDSSYNFNSAIEDFRSARQRASIQEVIARVMGRSNQLLSYDDV
                     AKKLRLQSRTERGLKNIPLDAIVGSVGRYTDFTRTFLPLQDSDRERWASVKAAMVTGA
                     GLPPIEVYQVGDVYFVLDGNHRVSIARQEGLKSIEAYVTEFRTDVKLTPETKLDEIII
                     KAEYIEFLEKTDLAQARPNVDLSVTIPGQYEKLMEQIDVCKYMDENETLTLQDAAANW
                     YDTLYIPLAEAIRDRELLQWFPGRTITDLYIWISENRAALEKELGWELRSDVAATDLI
                     LKRSVASESGSWRKARTIARYTDHLFEDILIPLSGQTESWDALYQGIVIAQRDQARLH
                     GLHIAKTKEEAEGAE"
     gene            1170..1643
                     /locus_tag="PLG52_10290"
     CDS             1170..1643
                     /locus_tag="PLG52_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41878.1"
                     /translation="MKQDKFLTGILIGIGALVVIALALFFTRQDKHDYLPEDTPDGVA
                     HNYVLAVLNKDYEKAYGYLADLKNKPTYEDFRQSFLNNMVNPDGTGADIGDATINGDE
                     AVVDVILYYSYSDPFSSRTGSPDRALLVKQDGEWKLSSMPYTFWDWNWYQEPYNP"
     gene            1687..>2766
                     /locus_tag="PLG52_10295"
     CDS             1687..>2766
                     /locus_tag="PLG52_10295"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HNB41879.1"
                     /translation="MKTIRRLYFYAVAAISIEVVLWGIIGLLRSILGAQDISNNATTL
                     ASALALIFVGVPIFLIHWGWAQNVSSKDSEEHSASIRAVFLYGILLGTLIPVVQNLLA
                     LINRTFLSTVHLSSYRAVVGGYQTWLDNLIAIIINLLIAAYFWNVLRSNWKTITEPES
                     FAEVRRLYRFVWVLYGLLMAVYGVQQAIAYAFSFPTGLLGEVGRETAVNAIALMVIGT
                     PIWYYTWRLLQDALTDTDEKESYLRLGLLYLLTLGGILVFLFSCGDLLYLIFLQVLGD
                     GKSASEFIRELGGALSVTVPFGVVWAYYSKWLNQQFAFDENLPRRAGKHRLYIYILSF
                     VGLTATTGGLISLFGVLIDLTLGNRY"
BASE COUNT          635 a          656 c          684 g          791 t
ORIGIN      
        1 cttcagcccc ttcggcttct tcttttgttt tcgcaatgtg taaaccgtgc agcctagctt
       61 ggtcacgttg tgcaatcaca ataccctgat aaagcgcatc ccagctttcg gtttgtccgc
      121 tgagggggat gaggatatct tcaaagagat gatcggtata acgcgcgatg gtacgggctt
      181 tgcgccaact gcccgattca gatgccacgc ttcttttcaa gattaggtct gtagctgcca
      241 catcagacct taactcccac ccaagctctt tctcaagggc ggcacggttc tctgatatcc
      301 aaatgtataa gtcggtgatg gtgcgaccgg gaaaccattg cagtagttca cggtcacgaa
      361 tggcttcagc gaggggaata tataaggtgt cgtaccagtt ggcggcggcg tcttgaagcg
      421 tgagggtttc gttctcatcc atgtatttgc aaacgtcaat ttgttccatc aacttttcgt
      481 attgcccagg gatggtgacg cttaagtcca cattggggcg agcttgagca agatcggtct
      541 tttcgagaaa ttcgatgtat tcggctttga tgatgatttc gtctaatttg gtttctggcg
      601 tgagtttgac gtcggttcta aactcggtga cataggcttc gatggatttc aagccttcct
      661 gccttgcaat tgagacgcga tgattgccat cgagcacgaa atacacatcg ccaacctgat
      721 acacctcaat gggtggaagc cccgctccgg tgaccatggc ggctttgacg cttgcccaac
      781 gttcgcggtc gctatcctgc aacggtaaga aagtgcgggt gaagtcggta taacgcccca
      841 cactgccaac aattgcatcg agcggaatgt tcttcagtcc acgctcagtg cggctttgca
      901 ggcgtaattt ttttgctaca tcgtcatacg ataacaactg attggaacgc cccataaccc
      961 gcgcgatcac ttcctgaatg gaagcgcgct ggcgggcact gcgaaaatct tcgatggcag
     1021 aattaaagtt gtatgacgaa tcagacatgc ttccgtaaaa cgttcctcat gaatgagtat
     1081 aaagtgaatg aagttcccct tcaagtatga aacttttgaa atgtcatttt cgtcaattta
     1141 gtgtacagtt aaattatgga ggtattctca tgaaacaaga taaatttctc accggcattc
     1201 ttattggtat tggtgccttg gtcgtaatag cgcttgcgct ctttttcacc cggcaagaca
     1261 agcacgacta tttgccagag gatacgccgg acggagttgc tcacaactat gtgctggcag
     1321 ttctcaacaa agattatgaa aaagcctatg gctaccttgc agacttaaag aacaagccta
     1381 cttatgaaga tttccgccaa tcgttcttga acaacatggt caaccccgat ggaaccggag
     1441 ccgacattgg cgatgcaacc atcaatggcg atgaagccgt ggtggatgtc attctctact
     1501 attcttacag tgatccattc tccagccgca caggcagccc agatcgtgcc ttgttggtga
     1561 agcaagatgg cgaatggaaa ctaagctcca tgccatacac attctgggat tggaactggt
     1621 atcaagaacc gtataacccg taatttttca tgtcattgca aaactcgtaa tgactgagga
     1681 tcaactatga aaacaattcg tcgtctttat ttttatgcgg ttgctgccat cagcattgaa
     1741 gtggtgttat ggggcattat tgggttgctg cgttccatcc ttggcgctca agatatcagt
     1801 aacaatgcca ccacacttgc ttcggcattg gcgctgatct ttgtgggcgt gcccatcttc
     1861 ctgattcact ggggttgggc gcagaatgtt tcttcaaaag actccgagga acattcagcc
     1921 agcattcgcg cggtttttct gtatggaatt ttgctgggca cgctgattcc ggttgtgcaa
     1981 aacttgttag cgcttatcaa ccgcactttt ctttccacgg tgcatctctc ttcataccgg
     2041 gctgtggtcg gcgggtatca aacttggttg gataatctca tcgcgatcat tatcaacttg
     2101 ttgatcgccg cctatttctg gaatgtgcta cgttccaatt ggaagaccat aaccgaaccc
     2161 gaaagcttcg cggaagtacg ccgtttgtat cgttttgtgt gggtgctgta tgggttgttg
     2221 atggcagtct acggcgtgca acaagccatt gcatatgcct tctcgttccc aaccggtttg
     2281 ctgggagaag tgggacgtga aaccgcggtc aatgcaattg ctttgatggt gatcggtacg
     2341 ccgatttggt attacacctg gcggctctta caggacgcgc taacggatac tgacgaaaaa
     2401 gaatcatatc tacgccttgg gctgctctac ttacttaccc tcggtgggat tctcgtcttt
     2461 ctattttcat gcggcgactt gttatacctg atctttttac aagtgctcgg ggatggcaag
     2521 tctgccagtg aatttattcg tgaattgggt ggggcgctct ctgtcaccgt tccgttcggt
     2581 gtggtgtggg catactatag caaatggctc aaccaacaat ttgctttcga tgagaatctt
     2641 ccacgccggg cggggaaaca tcgcctgtac atctacatcc tttcgtttgt ggggcttaca
     2701 gcaacgaccg gcggcttgat ctctctgttt ggcgttttaa tcgatttgac cttgggcaat
     2761 cgctac
//
LOCUS       DAOQNJ010000376         2748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_27505_length_2748_cov_7.901968, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000376 DAOQNJ010000000
VERSION     DAOQNJ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27505_length_2748_cov_7.901968"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLG52_10300"
     CDS             complement(<1..470)
                     /locus_tag="PLG52_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41880.1"
                     /translation="MLLLLLLILSLILSACGSSPAPTHAPTSGGFDIKPFLGVYEGTW
                     TNKTTGASGPAVIIVEAKEADKTVTLTLDFDGNYLGLNDPPAFPLTAKYDDNGAYVRG
                     ESEILGKMDITIDTQGNIVGNLSDLLGGTIPSMTYTGKIGNGHLDTDYIVTLSDG"
     gene            complement(709..1071)
                     /locus_tag="PLG52_10305"
     CDS             complement(709..1071)
                     /locus_tag="PLG52_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41881.1"
                     /translation="MKALRNTLIALAVYMTVVGALFLFAPSVAEKAFSTSLPDRALTP
                     LYGELMIVIALMAYLVSTDVSAYKKLVIAFIVSEIGHVLIFGYQLMTGIATFAQVGPP
                     MVIAIIFTVLLFVFKGKA"
     gene            complement(1294..1695)
                     /locus_tag="PLG52_10310"
     CDS             complement(1294..1695)
                     /locus_tag="PLG52_10310"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNB41882.1"
                     /translation="MSVETTKEVLTRYLNSGHSDQSMLAEDVSFTIMATGEEHRGREG
                     VSAMLNYFYHIAFEADAETRTLVFGETSAIWEGVIVGKHIGEYASVPATGKNVRVPIC
                     VVYDVEDKRVKRARVYFEVPAFYQQVGVKMG"
     gene            complement(1769..>2748)
                     /locus_tag="PLG52_10315"
     CDS             complement(1769..>2748)
                     /locus_tag="PLG52_10315"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB41883.1"
                     /translation="LGRARVLGKCAKVLGNLKEYEKSKLLFAQAEKELGIPPGEENIE
                     WWQTRLKVQFDQIWMIHDSYNIAGMQEVFEVVRPVVERLGETDMLAEYYFLLPTLYFN
                     SNGYQADEKIIEYSTLALELSLKGTDLELQSRTQFGYGFVQLLIGNIGLAIKHLEEGL
                     RLSTQIGYLDQQTYCITYLAVAHRRAGNLEECRQYSEQMLVISEREGQGSFTASAFAN
                     LGWLAWKQNDFAKAKTLSKKAAALWHPRYAFHWHGLWTLIDISLRSHKVDEAVENVRR
                     LRAPSQHVLSKELDDLMVDIIGQADKGNISNSESLLNQAVAWVKEHHYL"
BASE COUNT          696 a          701 c          656 g          695 t
ORIGIN      
        1 ccatcggata aagtcacgat gtaatcggta tcgagatgac cattaccgat cttgccggtg
       61 taggtcattg aggggatcgt tccgcccaat aaatcggaca agttgcccac gatattgcct
      121 tgggtatcaa tggtgatgtc catcttgcca aggatttcgc tctcgccgcg gacgtatgca
      181 ccattgtcgt catacttggc tgtgagcggg aaggctggcg ggtcgttcaa cccaagatag
      241 ttgccgtcaa aatcaagcgt gagggtcact gtcttatcgg cttctttcgc ttcaactatg
      301 atgactgccg gtccgctcgc gccagtggtc ttgttggtcc aagtgccttc gtagacgccg
      361 agaaacggct tgatgtcgaa tccgcctgaa gttggagcgt gagttggagc gggggaagaa
      421 ccgcaggctg acaatatcaa agacaaaata agcaaaagaa gtaataacat ttttctaatc
      481 accgttcctc ttttcttttt tccttgagaa cttcgctcaa aatatcatcg agcaatgtgt
      541 tcagcgactt ctcatcccca caataatgaa cctcgcccgt gtgtacattt tggagacgca
      601 cgcgccattg cggcgcgtgc gtgttctcat cggcactcca aagacgcaag gtaaacagga
      661 gcgattttgt gcgagcgcca gcctcatcgc tttccatatc acaacctttt aggctttgcc
      721 cttgaagaca aagagcagga cggtgaagat gatcgcaatg accataggcg gaccgacctg
      781 ggcgaaggtg gcaatgccgg tcattagctg gtagccgaag atgagcacat gcccaatttc
      841 cgaaacgatg aatgcaatga ccaatttctt gtaggcggag acatcggtgg agacgagata
      901 tgccatcagg gcgatgacga tcatcaattc gccgtagagc ggggtgaggg cgcggtctgg
      961 cagggatgta ctgaatgcct tttccgccac gctgggagca aagaggaaca aagctccgac
     1021 gaccgtcatg tagacagcca atgcaataag ggtatttcgt aacgctttca taagagcctc
     1081 cgtaaatgag ttgaaatatc gaactgtgct cattttatgg atggggcgtt tcaaaagcgt
     1141 ttcatggagc gtttctcggg aaagaaaatc cccgcctaga aggcggggat catagctttt
     1201 ttcaccttgt atatttttaa cgcaaaggcg cagggtcgca aagaaaggaa tttgaaacat
     1261 ggcggcttag cgactttgca ttaagagggg aggttatccc attttgacgc cgacctgctg
     1321 ataaaaagca gggacttcaa aatagacccg agcccgcttg actctcttat cctccacgtc
     1381 atacaccaca cagatcggca ctcggacatt ctttcctgtg gcaggtacgc ttgcatattc
     1441 gccaatgtgc ttccccacaa tgacaccttc ccaaatggcg ctcgtttcgc cgaagaccag
     1501 ggtgcgcgtc tcggcatctg cttcaaaagc gatgtgatag aaataattca gcatcgccga
     1561 aacaccttct cgaccacggt gttcctcacc ggttgccatg atggtaaagc tgacatcctc
     1621 tgccagcatg ctctggtcac tgtgaccaga gttgaggtag cgtgtcagta cttcttttgt
     1681 agtttcgaca gacatggtgc ttctccttaa gttgttgagt tgcccccagt gtacggagaa
     1741 gccgtttcaa aaccgtttca ttggaacgtt acaggtaatg atgttccttt acccaagcca
     1801 cggcttgatt tagcaaagat tcactgttgg aaatattacc cttatccgcc tgaccgataa
     1861 tatcaaccat tagatcatcc aactctttcg ataacacatg ctgactcggt gccctcagcc
     1921 gacgcacatt ctcaaccgcc tcatccactt tgtgagaacg caggctgata tcgatcaacg
     1981 tccacaagcc atgccagtgg aaagcataac gcggatgcca gagtgcagcc gcttttttgg
     2041 aaagtgtttt tgctttggca aagtcatttt gtttccatgc cagccagcct aaattggcaa
     2101 acgcggaagc ggtaaaactg ccctgccctt cccgttcgct gattaccagc atttgctcag
     2161 agtactggcg gcattcttcc aaatttccag ctcgccgatg cgcaaccgcc aaatacgtaa
     2221 tgcaataggt ctgctgatcc aagtatccga tctgcgtcga gaggcgcaga ccttcctcca
     2281 ggtgtttaat tgcaagtcca atatttccta tcagcagttg tacaaaccca taaccgaatt
     2341 gagtacgtga ctgcaattcg aggtcggttc ctttgaggct tagttctaaa gcaagcgtgg
     2401 aatattcaat gatcttttcg tctgcctgat agccattgct attgaaatac aaagtaggca
     2461 acaggaaata atattcggca agcatgtccg tctcgccaag acgctcgaca acaggacgaa
     2521 cgacctcaaa gacttcctgc atccctgcga tgttatatga atcatggatc atccatatct
     2581 ggtcaaactg gaccttgagc cgggtctgcc accattcaat attttcctcg ccgggaggga
     2641 tgccaagttc tttttcagcc tgagcgaata gcaacttgga cttttcatac tccttcaaat
     2701 tacccaatac cttcgcacac tttcccaaca cgcgagcccg tccgagaa
//
LOCUS       DAOQNJ010000377         2740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_27627_length_2740_cov_9.531471, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000377 DAOQNJ010000000
VERSION     DAOQNJ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27627_length_2740_cov_9.531471"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /gene="thrS"
                     /locus_tag="PLG52_10320"
     CDS             <1..1226
                     /gene="thrS"
                     /locus_tag="PLG52_10320"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNB41884.1"
                     /translation="KKRDHRKLGKDLEIFIFDEEVGPGLPLWLPNGGIMIEELEKLAK
                     EMEEKAGYDRVKTPSVTKEDLFLRSGHLPYYAESMFPPMELEGVKYYVKPMNCPMHHK
                     IFGSKGRSYRDLPVRLAEYGTCYRYEKSGELFGLMRVRSMQMNDAHIYCSEEQFEQEF
                     MGVVELYKKYFELFGIEKYVMRLSLHSKAGLGKKYVDNPELWIKTEDMVRRAMDNGGV
                     PYVEAEDEAAFYGPKIDVQIWSAIGREFSLATNQVDFAVPSRFDLKFTNSEGHDEVPL
                     CIHRAPLSTHERMVGFLLEHYAGNFPVWLSPEQVRVISITDEQNDYAEKIAKELRENG
                     IRASADMSSQRMNAKIRNAQMMKVPYMIVVGKNEMEAGQISLRVRDGSQQNGFALSEF
                     IARAKDRISRRASEL"
     gene            1326..1856
                     /locus_tag="PLG52_10325"
     CDS             1326..1856
                     /locus_tag="PLG52_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNB41885.1"
                     /translation="MSTDSQPKRYHPVHVTLHWLTALLVFMPLLSGFLILARTPDAQE
                     AQFLGMHKWIGIFLGLVIIARIITRFVFKRPAPADAGHPLLNWVGAIVHFLLYAGIFA
                     MLFSGDALDEAYGLEGILAGNGTMPESVMVYAEWARHSLFSYTLAALVALHVGAALYH
                     QFIRKDNLLSRMWYGK"
     gene            complement(1911..2705)
                     /locus_tag="PLG52_10330"
     CDS             complement(1911..2705)
                     /locus_tag="PLG52_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41886.1"
                     /translation="MELNFATITLPTIVVWMLGLFIFLLGSLLGYFNMSIDARKKQEL
                     AEQKVEHARIEADRRIAEMQKRLDEADIQPAKVQQTATTPSLLTLKSVNNLQTQIEMD
                     GQLLIAPLSPERKKRLVELINHFRPWLEGAATSQPISKPLTTPLSGPPTDLKKPEAVP
                     YVPPTVKPVPVILGVNNQKTKTDPESEFKLLSMVKQIDVVLQKRLAGTSLESLGIHLN
                     DSLQGGLEVQIGSQKFETIDDVPDANIKAAIRAAIAEWEQKYIPGQ"
BASE COUNT          680 a          612 c          695 g          753 t
ORIGIN      
        1 ccaagaaacg cgaccatcgc aaactgggca aagacttgga aatcttcatc ttcgacgaag
       61 aggtgggacc aggtttgcct ttgtggttgc cgaatggtgg catcatgatc gaagaactcg
      121 aaaagcttgc caaagagatg gaagaaaagg ctggctacga ccgagtcaag acgccgagtg
      181 tgacgaagga agatctgttc ctgcgctctg gtcacttgcc ctattatgct gagagcatgt
      241 ttccgcccat ggaattggaa ggcgtgaagt attacgtcaa accgatgaac tgccccatgc
      301 atcacaagat attcggctca aagggacgct cctaccgtga cttgcctgtc cgcctcgctg
      361 aatacggcac gtgttatcgc tacgaaaaat caggcgagct ctttggtctg atgcgcgtcc
      421 gctcgatgca gatgaacgat gctcatatct attgttccga agaacagttc gagcaggagt
      481 ttatgggtgt ggtcgaactg tacaagaaat actttgaatt gttcggtatt gagaagtatg
      541 tgatgcgcct ctctcttcac tcgaaggcgg gtctcggcaa aaaatacgta gacaaccctg
      601 agctgtggat caagaccgaa gacatggtgc gccgcgctat ggacaatggt ggcgttcctt
      661 atgtggaagc tgaagacgaa gcagcattct acggtccaaa gatcgatgtg cagatttggt
      721 ccgcgattgg acgtgagttc tcgcttgcta cgaatcaagt ggactttgcg gttccttctc
      781 gctttgacct gaagttcacg aacagcgaag gtcacgatga agtgccgctt tgtattcatc
      841 gtgcgccgct gtccacgcat gaacgcatgg tgggcttcct gcttgagcat tacgcgggca
      901 acttcccagt ttggctctcg ccggaacagg tgcgcgtcat ctcgattacg gatgagcaga
      961 atgactatgc cgaaaagatc gcgaaggaat tgcgcgagaa cggcatccgt gccagtgcag
     1021 acatgtcgtc ccagcgtatg aatgccaaga tccgcaatgc gcagatgatg aaggtgccat
     1081 atatgattgt ggttggcaag aatgaaatgg aagccgggca gatctcgtta cgtgtccgcg
     1141 acggtagcca gcaaaatggc tttgccttaa gcgaatttat tgcccgggcg aaagatcgca
     1201 tctcccgccg cgcaagtgaa ctgtaatttt caacaccgga gaaatacatc tccggtgttg
     1261 tctttctatt gaaactctga aaacgatttg gggttttatc tgaaaatcaa aaccaaggag
     1321 agattatgtc tactgattca cagcccaaac gctatcatcc cgtccacgtt accttacatt
     1381 ggctgaccgc acttttggtc tttatgcctt tgctttcagg tttcctgatc cttgcccgca
     1441 ctccggatgc tcaagaagca caattcctcg gcatgcataa atggatcggt atctttctcg
     1501 gtcttgtcat tattgcccgt attattaccc gcttcgtatt caagcgtccg gctccagcag
     1561 acgctgggca tccgcttttg aactgggtgg gtgctattgt ccacttcttg ctatatgcag
     1621 ggatttttgc catgctgttc tcaggcgatg cgcttgatga agcctatggt ttggaaggca
     1681 ttttagcagg caacgggacc atgccagaaa gtgtgatggt ctatgccgaa tgggcaagac
     1741 atagtctgtt ttcgtacact ctggcggctt tggtcgcttt gcatgtgggc gcggcgttat
     1801 atcatcaatt tattcgcaag gataatttgt tgtctcgtat gtggtacggg aagtaaaaag
     1861 catataacag taaaaaagcc ctgctcacaa gagcagggct tttttcgaaa ttattgtcca
     1921 gggatatact tctgttccca ttctgcaatt gctgcacgga tggcagcttt gatatttgca
     1981 tcgggtacat catcaattgt ctcaaatttt tgtgaaccaa tctgcacttc caagccacct
     2041 tgtaatgagt cgttgagatg aatgcccaga gactctagag acgtccctgc caaccttttt
     2101 tgtaatacaa cgtcgatctg cttaaccatg cttagtaatt tgaactcaga ctcagggtct
     2161 gttttggttt tttgattatt cactccaaga atgacaggaa ctggctttac agtaggtggc
     2221 acataaggaa cagcctcagg cttcttaaga tcagttggag gtccagaaag gggcgtagtg
     2281 agaggtttgg aaataggctg ggatgttgca gccccttcaa gccacggacg aaaatgattg
     2341 ataagttcca ctaaacgttt ttttcgttca ggagaaagag gggcgattaa tagttgccca
     2401 tccatttcga tttgagtttg tagattattt acgcttttta atgttagcaa cgaaggagta
     2461 gtggcagttt gctgaacctt ggcaggctgt atatcagctt catctaaccg tttttgcatt
     2521 tcagcaattc tacgatctgc ttcaatacgc gcatgttcta ccttttgttc tgccagttct
     2581 tgtttcttgc gtgcgtcaat gctcatattg aaatagccaa gtaagctgcc aagtaaaaag
     2641 ataaaaagtc ctagcatcca tacaacgatg gtgggaagag tgatagtggc gaaattaagc
     2701 tccatggtta tttcctttca agcggctggc aggttgggca
//
LOCUS       DAOQNJ010000378         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_27683_length_2737_cov_7.855705, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000378 DAOQNJ010000000
VERSION     DAOQNJ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27683_length_2737_cov_7.855705"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PLG52_10335"
     CDS             complement(<1..1441)
                     /locus_tag="PLG52_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41887.1"
                     /translation="MSNNLQRITTVVLILLAALLIAQAAFSLQWPIAHDEAPLFYEAF
                     LMQNGQVPYKDFFDFQMPGSFFAYYILGILSNFGPLRIRILDLIILVAISTITYFAMR
                     KRFGMSIALAAPILFALKYLQGGPNLSLQREYIVLIFISLSIWIGVTDSLTFRKRLML
                     GLLYGLAATLKPHAALGLLPFLLFDIADLIQRRELTLPSLEKQSILPTTIGFAIPISL
                     ITLYLTITHSLFPLVDIILNYVPLYTQINGEMAITPSAERTAYLLNQLWHLGGSGLWL
                     IPAAFGIYLNRNRQTYLLASLAAIYAIYPALTGQFFPYHYIPFIYTIILVASLSLSSN
                     YQLPITNLAYIFLFAIILITIRPSQAFLRQLEGKDIVIAADRAAQISAFLEKNLEPGD
                     QVQPLDWTGGSLLAMLETRAPIATSYIFDFYFYHHISNPYIQSLRADFMTQLQESKPR
                     FIIEVTAIDKPWVSGEDTSRDFPELRAFLN"
     gene            complement(1431..2126)
                     /locus_tag="PLG52_10340"
     CDS             complement(1431..2126)
                     /locus_tag="PLG52_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41888.1"
                     /translation="MKRSPNFLLACVLSFSMIACGRGILPQQSSVIAEAPDTPKPIEK
                     TLSSFTPIIGTDYLMAGIVPIEVTRDSTLNPFEWISSSGYSSYTSYATYNYVFFNTQT
                     EEYHRLLPDNNSVILQTAGFPQQVYDPNDPQKPAPVIEFWMFNIIKADTNGDTYFDYR
                     DKLTIGISDVSGSGYTELIQNMDVIQSQYYKDPSNFFIIYNVNDKNFIAKVNPITRQI
                     LSTTEMDLGEDVK"
     gene            complement(2139..2597)
                     /locus_tag="PLG52_10345"
     CDS             complement(2139..2597)
                     /locus_tag="PLG52_10345"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNB41889.1"
                     /translation="MTDSQIETYWEAFLAAHPEYRSKPYLVEPFGDNPALADELGNLV
                     LSGLKSATCSAVWNYEAEGQPFPQVGTLWLVLSGRNEPMCVVETVEVTFRKYNEVDAD
                     FARAEGEDDLSLESWRAGHKRFFTRMLATIGKEFVEDMPLVCERFRVIYK"
BASE COUNT          683 a          505 c          872 g          677 t
ORIGIN      
        1 cattcagaaa cgcgcgtagt tcgggaaagt ctcggctggt gtcttcgccg gacacccagg
       61 gcttgtcgat ggcggtgact tcgatgatga agcgtggttt ggattcttgc aactgagtca
      121 tgaagtctgc gcgcagggat tggatgtagg gattggatat gtgatgatag aaatagaaat
      181 caaagatgta cgaggtggca atcggtgcgc gagtttcgag catggcgagc agggaaccgc
      241 cggtccaatc caacggttgg acttggtcgc cgggttcgag atttttttcg agaaaggcgg
      301 atatctgcgc ggcgcggtca gcagcaatga cgatgtcttt gccttcgagt tggcgaagga
      361 aggcttggga agggcggatg gtgatgagga tgattgcgaa gagaaagata tacgcgagat
      421 tggtaattgg taattggtaa ttggatgata gcgaaagtga tgcgacgaga atgatggtgt
      481 agataaaagg aatgtagtga tagggaaaga actgtccagt cagcgcggga tagatagcgt
      541 aaatggcagc gaggctggca agtagatagg tttgacggtt acgattaagg tagatgccga
      601 aagcggcggg gatgagccag agtccgcttc cgccgagatg ccagagttga ttcagtaaat
      661 aagccgttct ctccgctgac ggtgtgatcg ccatctcgcc gttgatctgg gtgtagagtg
      721 gaacgtagtt gaggatgatg tcgactaatg ggaataggga atgggtaatt gttaagtaaa
      781 gagtgatgag tgaaatggga attgcgaagc cgatggttgt tggcaggatg ctctgctttt
      841 caagcgatgg cagagtcaac tcacggcgtt gtattaggtc tgcaatgtca aagagcagga
      901 aggggaggag tccgagcgcg gcgtgcggtt tgagggtggc ggcgagtccg tagaggagtc
      961 ctagcatgag gcgtttgcga aaggtcagag agtcggtcac gccgatccag atcgaaagag
     1021 aaatgaaaat gaggacgatg tattcgcgtt ggagcgagag gttgggaccg ccttgcaagt
     1081 atttgagcgc gaagagaatg ggcgcggcaa gggcaatact catgccgaag cgttttcgca
     1141 tcgcgaagta tgtgatggtt gagatggcga caaggatgat gagatcgaga atgcgaatgc
     1201 gaagcggacc gaagttgcta aggatgccga ggatgtagta agcgaaaaaa cttccgggca
     1261 tttggaagtc gaaaaagtct ttgtagggga cttgcccgtt ttgcatgagg aaggcttcgt
     1321 agaagagcgg ggcttcgtcg tgcgcgatgg gccattgaag cgagaatgcc gcctgtgcga
     1381 tgagcaaggc ggcaagcagg attagaacga ctgtggtgat gcgttgaaga ttatttgaca
     1441 tcttctccaa ggtccatttc ggtggtggag aggatctggc gggtgatggg gttgactttg
     1501 gcgatgaagt tcttgtcgtt gacgttatag atgatgaaga agttggatgg gtctttgtag
     1561 tattgcgatt ggatcacatc catattttgg attagttcgg tatagccgct gccgctaaca
     1621 tcggagatgc caatggtgag tttgtcgcga taatcaaagt aggtgtcgcc attggtatcg
     1681 gctttgatga tgttgaacat ccaaaattcg atcacgggcg cgggcttttg cgggtcattg
     1741 ggatcataca cttgttgagg gaacccggca gtttgcaaga tgacagagtt attatctggc
     1801 aggaggcgat gatattcttc ggtttgggtg ttgaagaaaa catagttata tgtggcgtag
     1861 gatgtgtaac ttgagtaacc gctactgctg atccactcaa agggattgag agtggagtcg
     1921 cgggttactt caatgggcac aatgcctgcc atgagatagt cggtgccgat aatgggagtg
     1981 aatgaggaaa gagtcttttc aatgggtttg ggggtatctg gcgcttcggc aatgacagat
     2041 gattgttgcg gtaggatgcc gcgtccacag gcgatcatgg agaacgatag gacacatgca
     2101 agcagaaaat ttggagagcg tttcatgagg acctcacttt atttgtaaat gacgcggaag
     2161 cgttcacaaa cgagcggcat atcttctaca aattcttttc cgattgttgc aagcatacgg
     2221 gtgaagaagc gtttgtgccc tgcacgccag ctttccagag aaagatcatc ttcgccttcg
     2281 gcacgggcaa aatctgcatc cacttcattg tatttgcgaa aggtcacttc gacagtttca
     2341 accacgcaca tcggttcatt gcgtccgctg agcacgagcc atagtgtgcc cacttgtgga
     2401 aacggctgac cttcggcttc gtagttccac acggcagaac aggtggcgga tttaagtcca
     2461 ctgaggacaa ggttgccgag ttcatcggca agggcggggt tgtcaccaaa gggttcaaca
     2521 aggtagggtt tgctgcgata ttcaggatgg gcagcaagga acgcttccca ataggtttca
     2581 atttgcgagt cggtcactcg atgaaatctc cgccgatgat gtctttttcg atcacttggc
     2641 gatctgcatt gaacttgagg tttacttcta tacgctgaaa gcatttgttc tcgcccatta
     2701 atactttgcg agcatagaaa acatcgccgc catcttc
//
LOCUS       DAOQNJ010000379         2724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_27920_length_2724_cov_7.146497, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000379 DAOQNJ010000000
VERSION     DAOQNJ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27920_length_2724_cov_7.146497"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PLG52_10350"
     CDS             complement(<1..1133)
                     /locus_tag="PLG52_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB41890.1"
                     /translation="MDIDFDLYRYEVRISSNPLVRLSAIDVSPSSPKHTIVFIHGFGG
                     KAEQWQYQMQKFAMDNRVIALDMRGHGLSDKPSTGYDMERIQLDLETALTQLKVSTPF
                     VLVGHSFGGAVVTEYALKHPDHIEKLIIIATAGEFRLSPLFKLGLNLPSSVLRLIGPF
                     TRKWLHAPPHALREFYHSNMAKWRGWDRFAQLTVPTLVIRGHRDIVFDRPLFEKVAAS
                     IPGAEDADIGVSGHMVMLERREAVDRAIANFLKGDAKKSWRDKSFIIPRTQRDDLKTE
                     RNWLNHYEEGVPYTVDVPRIPAHHLLRSAVRRFPNRTALFFEGAKMTYRELNHEVNRF
                     ANALLAQGIGKGARVVLLLPNIPQMVIGFYGTLKAGGAAVLIPP"
     gene            1204..1530
                     /locus_tag="PLG52_10355"
     CDS             1204..1530
                     /locus_tag="PLG52_10355"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HNB41891.1"
                     /translation="MSFEKAVETIIREAQERGDFDNLKGKGQPLDLDAYFETPEDLRL
                     AYSALKNAGLVSPEVELLQEIAALKERLTTTYEEAQRNKIKKIIHDKQLEFNILLERQ
                     KRHRKG"
     gene            1836..>2724
                     /locus_tag="PLG52_10360"
     CDS             1836..>2724
                     /locus_tag="PLG52_10360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNB41892.1"
                     /translation="MSKKNKLRIIPLGGLGEVGKNMMAYEYGNDIIVVDAGIMFPKND
                     MLGVDYIIPDYEYLKNKSNRILGLFITHGHEDHIGAIQHFIEDIPVPVYATPLTRGLI
                     EAKLHRNGAQHKAQLKTINAGDATRVGPFTLEYFHVNHSIPDAVGIGITTPAGLVVHM
                     SDFKFDQTPVDGWPTDFAKLAEFASRGVDLLLSDSTNAERPGWTPSEAVIQPAFEKVF
                     AEAKGRVIVATFASLISRVQQVADAAVKYGRKMALAGPSMVDNVKIARKMKYLEIPDE
                     IIVPIEQALQMQNHKVVIMC"
BASE COUNT          720 a          697 c          684 g          623 t
ORIGIN      
        1 ggcgggatca aaactgccgc tccgcccgct ttgagggtgc cgtaaaagcc gatcaccatt
       61 tgtggaatat tcggcagcaa caaaaccacg cgcgcgcctt tgccaattcc ctgtgccaac
      121 aaggcattgg caaaccgatt gacctcatga ttcaactcac ggtaggtcat cttcgcgcct
      181 tcaaagaaga gcgccgtccg attcgggaag cgccgaactg ccgaacgcaa gagatgatgt
      241 gccggaatac gtggcacatc caccgtgtag ggtacgcctt cttcatagtg attcagccag
      301 ttgcgctcgg tttttaggtc atctcgttgg gtccttggga tgatgaaaga cttgtcacgc
      361 caagattttt tcgcgtcgcc tttgaggaag tttgcaatcg cacggtcgac cgcttcacgc
      421 ctctctaaca tgaccatatg accggagacg ccaatatctg catcttctgc gccgggaatg
      481 gaagcggcta ctttttcaaa gagcgggcgg tcaaagacga tatcgcgatg tccgcgaatg
      541 acgagggttg gcacggtgag ttgagcaaac ctatcccaac cgcgccattt cgccatgttg
      601 ctatgataaa actcacgcaa ggcatgtggc ggggcatgca gccatttacg cgtgaatgga
      661 ccgatcaacc gcagaaccga agaaggcaag ttaagcccca gcttgaagag cgggctcagc
      721 ctaaactcgc cagctgtggc aatgatgatc aatttttcaa tatggtcggg gtgcttcagg
      781 gcatattcgg ttacaacagc gccaccgaaa gaatgcccaa ccaaaacaaa cggcgtggac
      841 actttcaact gggtcagagc cgtttctagg tccagttgga tacgttccat gtcatagccc
      901 gtggacggtt tatcggagag tccatgtccg cgcatatcca aagcaatgac gcgattatcc
      961 atcgcaaact tttgcatttg atattgccat tgctcagctt tgccgccgaa gccatggata
     1021 aagacaatag tatgttttgg gctggagggg gaaacgtcga tggcggagag ccgtaccaac
     1081 gggttggagg aaatgcgtac ttcgtaacgg tagaggtcga agtctatgtc cattagagaa
     1141 gtgtacaaga gacgagggag attgtcaatt ttctttgtga aataaggtat ataatggcgc
     1201 aatatgagtt tcgaaaaagc agtggaaacc atcattcgtg aagcgcagga acgtggcgac
     1261 tttgacaacc tcaaaggcaa ggggcagcct cttgacctgg atgcttactt tgagaccccc
     1321 gaagacttac gcctggcgta ttcggcatta aagaatgccg gcttggtctc gccagaagtg
     1381 gaactattgc aagagatcgc cgctctcaaa gaacggctga cgactacgta cgaagaagcc
     1441 caacgaaata agatcaaaaa gatcattcac gacaagcagt tggagttcaa cattctgctg
     1501 gaacgccaga aacggcaccg caaagggtag ccaaagcttt aacgattcta taacacgccc
     1561 gctttgacaa aacccgcccg ccctgctata ctctttgaca gttaagttga cttttcctgc
     1621 cgaattcggc agtttgtttg agaacaatcg tgttttaccg gcatcgatct tcgcgagcaa
     1681 gtcagcgaag gtctttttgt ttgtttgagg gaaaagacgt ttactggaat ctgcaagcct
     1741 ttcttgcaag aaactcctac ccaacaccta ccttgacttt aaccgcatag gcggtttcta
     1801 atctttgaaa tctgtgaggt atacagaaaa attatatgag taaaaaaaat aaattaagaa
     1861 ttatcccgct cggggggctg ggcgaagtgg gaaagaacat gatggcgtat gagtacggca
     1921 acgacatcat cgtggtcgac gctggcatca tgttcccaaa gaacgatatg ttgggcgtag
     1981 attacatcat cccagactat gagtatttaa agaacaaatc caaccgtatt ttagggttat
     2041 ttatcaccca cgggcatgaa gaccacatcg gcgcgataca acactttatt gaagatattc
     2101 cggtcccggt ctatgccacc ccgctcaccc gcgggttgat cgaagccaaa ttgcaccgca
     2161 atggggcgca acacaaagct caactaaaaa ccatcaatgc tggtgacgcc acccgcgttg
     2221 gtcctttcac cttggaatat ttccacgtca atcactcgat ccctgatgct gttgggatcg
     2281 gcatcaccac cccggcggga ctcgtcgttc acatgagcga tttcaagttc gatcaaaccc
     2341 ccgtcgacgg ttggcccacc gactttgcca aactcgccga gttcgccagc cgcggtgtgg
     2401 acctgttact ctccgactca accaacgccg aacgccccgg ttggactccc tctgaagcgg
     2461 tcattcagcc cgctttcgaa aaggtcttcg ccgaagcgaa aggacgtgtg atcgttgcca
     2521 cctttgcttc gctcatctcg cgcgtgcaac aggtcgcaga cgccgccgtc aagtacggac
     2581 gcaaaatggc gttagcgggt ccgagcatgg ttgataacgt caagatcgcc cgcaagatga
     2641 aataccttga aatccccgat gaaatcattg ttcccatcga gcaggcatta cagatgcaaa
     2701 accacaaagt ggtcatcatg tgta
//
LOCUS       DAOQNJ010000380         2719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_27996_length_2719_cov_8.488363, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000380 DAOQNJ010000000
VERSION     DAOQNJ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27996_length_2719_cov_8.488363"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..759)
                     /locus_tag="PLG52_10365"
     CDS             complement(136..759)
                     /locus_tag="PLG52_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB41893.1"
                     /translation="MKILAVSDQVVERVYSLAPSGHFKGVELILGCGDMPYTYLEYLV
                     TILSVPLFYVPGNHDPDFNPRDHRSRAEGGSNLDLRLVYHNKSLIGGFGGSIRYRPDG
                     TNQYTQNEAFLRAFNMVPKLVMNRIKYGRALDILISHSPPFGIHDDTDYAHNGLKALN
                     WLLDFAKPRYHFHGHTHFYRNNIEQSITKTSTTQIMNVYPYKVVDIE"
     gene            884..>2719
                     /locus_tag="PLG52_10370"
     CDS             884..>2719
                     /locus_tag="PLG52_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB41894.1"
                     /translation="METRNITSPIGPYGSGSGSGSGDQAGRQQLIPGVTLVNRYSIQD
                     VIGVGGMGSVYRARDMHFPNVVKLVAVKEMINMAPDPLVRQTIVQNFEREANLLATLH
                     HPSIPRIYDYFSHDDRSYLVLEFVQGRDLETVINEANGFLTEDQVISWGIQLCDVLEY
                     LHGHKPDPIIFRDMKPSNVMINQNGDVILVDFGIAKTFQTGIKGTMIGTEGYSPPEQY
                     RGDATPLADVYALGATMHHSLTRRDPRLEPPFTFAERPIRRINSNVSIELEAVVNTAL
                     NYNPTERFPTAASMKEALMNVARKTGTLSKLSAVLPTPASSAIKPIWDFKCEDEIRGT
                     PTLSQGSLYIGCYDNNLYSLNAADGKFQWKYPTDGGIVSRPLVFENNVFFCSEDQRLH
                     VIGARTGKLLWTYYTEGKIYSSPRISDGHVFFGSEDHDLHAVNVNSGRAVWKFSTDGP
                     IYSTPLVTNEMIYFGSESGSFYGLDFRGAMKWRFAAKRGMTASPTSKGQAIYNASLDG
                     TLYALDAKSGWAIWKFRLGKGSVSSPVIMDDLIFLGAADGFVYCVDIRTSKEVWRYKT
                     DNQVSSSPTIYKDSLYVGSVDGAMYCLEYRTGRLRWKFETGGPITG"
BASE COUNT          715 a          603 c          619 g          782 t
ORIGIN      
        1 ctaaaatctg cacgtatttt tgaatctatt acgtctgaca tttctttctc tctttgctaa
       61 taacaattca gtctattata taaaacatac taataaataa gttattgtga gattaatatc
      121 cgattaggat atgtcttatt caatatcgac aaccttgtaa ggatagacgt tcatgatctg
      181 agtagtgcta gtttttgtta tgctctgctc gatattattc cgataaaagt gcgtatgacc
      241 atgaaaatga tagcgtggtt ttgcaaaatc taacagccaa tttaatgctt ttaacccatt
      301 atgggcataa tctgtatcat catggatccc aaatggggga gaatggctaa tgagaatatc
      361 cagtgcgcgc ccatacttga tacgattcat gaccaatttg ggtaccatat tgaatgcccg
      421 taagaaagct tcattctgtg tatattgatt cgtgccatcg ggtctgtagc ggatggaacc
      481 gccaaagccg ccgatcaagc tcttgttgtg gtagaccagt ctcaagtcta gatttgagcc
      541 gccttctgct cgagaacggt ggtcacgcgg gttgaagtct gggtcatgat tccccggaac
      601 ataaaaaagt ggcacactca aaatggtgac gaggtattca aggtaggtgt agggcatgtc
      661 tccacaccca agaatcagtt caacaccttt gaaatgtccg ctcggcgcca gcgagtagac
      721 acgctctacc acctgatcac ttactgccaa tatcttcaca ggggctcatt gtgccgtaga
      781 aaacgctcga ttgcaacccc aaaagtgagc tttttgcaaa attgcaatag ccagcatcgg
      841 caaataccct tataattcat taaatttttg gaaaaaaaca cagatggaaa cgagaaacat
      901 tacctctccc attggtcctt atggctcggg ttccggctca ggctccggtg atcaagccgg
      961 acgtcagcaa ttaataccag gcgtgaccct tgtcaaccgc tatagcattc aagatgttat
     1021 aggcgttggt ggtatggggt ctgtgtatcg cgctcgggat atgcatttcc cgaatgtggt
     1081 taagctagta gctgtcaaag aaatgatcaa tatggcacca gatcccttgg ttcgccagac
     1141 gatcgtacag aactttgagc gtgaagccaa tttgttggct acactgcatc atccatctat
     1201 tccacgtatt tatgattatt tctcccatga tgatcgttct tatcttgttt tagagtttgt
     1261 tcaaggtaga gaccttgaaa cggttatcaa tgaagcaaat ggctttctga cagaagatca
     1321 ggtaattagc tggggaatcc agctttgtga tgtgttggaa tatctgcacg gtcataagcc
     1381 agatcccatc atttttcgtg atatgaaacc ctcgaacgtg atgatcaacc agaatggcga
     1441 tgtcattttg gttgacttcg gtatcgccaa gacattccaa accggtatta aaggcacgat
     1501 gatcggaacg gaaggatatt cgccacctga gcaatatcgt ggggatgcca cgcctcttgc
     1561 ggatgtgtac gcgctgggtg ccaccatgca tcattctctt acccgacgcg acccacgcct
     1621 cgaacctcct tttacttttg ccgaacgccc aatccgccgc attaactcga atgtttccat
     1681 tgagttagaa gctgtggtaa atacggcgtt gaattacaac cctactgaac gcttcccaac
     1741 agcagcgtcc atgaaggaag ctttgatgaa tgtggctcga aagacaggaa ccctttcaaa
     1801 gctttctgcg gttttgccga cccctgccag tagtgcgatc aagcctattt gggattttaa
     1861 gtgtgaagat gaaattcgcg gcacaccaac attgagtcag ggatctttgt atattggctg
     1921 ttatgacaat aacctttatt ccctgaacgc tgctgatgga aagttccagt ggaaataccc
     1981 gactgatggc ggcatcgttt cccgcccatt ggtttttgag aataatgtct tcttttgttc
     2041 tgaagatcaa cgcttgcatg taattggtgc ccgcacaggc aaattactgt ggacttatta
     2101 cacagaagga aagatttatt cttctccacg catctcagac gggcatgtct ttttcggctc
     2161 cgaagaccat gaccttcatg ctgtgaatgt gaatagcggt agagccgttt ggaagttttc
     2221 taccgatgga cctatctact ctactccgct agtaacaaac gaaatgatct acttcggatc
     2281 agagtcaggt tcattctatg ggcttgattt tcgcggcgcg atgaaatggc gtttcgctgc
     2341 caagcgtggt atgactgctt cgccaaccag taaaggacaa gcgatctaca atgcatcttt
     2401 ggatggaacc ctctacgcac ttgatgcaaa gagcggctgg gcaatctgga agttccgttt
     2461 agggaagggg tccgtctctt caccagtcat tatggatgac cttatcttct tgggtgcagc
     2521 agatggcttt gtatattgcg ttgatattcg gacttccaaa gaagtttggc gctataaaac
     2581 agataatcaa gtgagtagtt cacctaccat atataaagac tctctttatg ttggctctgt
     2641 tgacggcgct atgtattgcc ttgaataccg tactgggcgt ttacgttgga aatttgaaac
     2701 aggcggtccc attactggc
//
LOCUS       DAOQNJ010000381         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28086_length_2713_cov_12.275771, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000381 DAOQNJ010000000
VERSION     DAOQNJ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28086_length_2713_cov_12.275771"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(473..697)
                     /locus_tag="PLG52_10375"
     CDS             complement(473..697)
                     /locus_tag="PLG52_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41895.1"
                     /translation="MAYTLDTKVGEILDDTGAVKILEKHVPGVSKNPLIGLASGMTLK
                     QILSMPQAKEAGLTEEMVKKVLAEINALKK"
     gene            complement(813..>2713)
                     /locus_tag="PLG52_10380"
     CDS             complement(813..>2713)
                     /locus_tag="PLG52_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41896.1"
                     /translation="ALVNSVNTEEADLKLFFRELERIGKGEISTQAFTAAKQALKAEL
                     ESEPEWLYEEKKFALDQALFNSLNIKDVYKYIFNAESKGDIEVLVRPSNDKELAFKLK
                     TSAAPFALIKIGQTTEWMKDFLAGYEIVQGFEDESFFARLNEEDSEINLLMGSRSFYE
                     GWDSNRPNVITFINIGTGSEARKFVLQSVGRGVRIEPMKGKRKRLENLHNANEVDDML
                     FGQAKPFLAAVQSLFIFGTKREALETIFEGLAQEKEKEEGVELELSVNPDAISNYPVL
                     IPVYRKLNGHLLVEQHDPRKFEIQASESALLKQYVEYLGDERLLLARHGMTPQQIGLL
                     DETLKSPDSYFNPNTQKKYGNINLLLPRLTQYFGLIPQEVEGFKPLEDEINHFKHIRV
                     LLKDLLEELKKKIEAVRLYKDPADAEKNLKKELKDGTLDIDAYTLAVKNLARTEPSEI
                     FEHNGQRLHIKNIASHYYLPLLVSNDDRLDYISRVIKVESERKFVNQLEAYIKDENHL
                     LNQYDWWMFSRAEETLDKLTIPYYDPTSNKMRDFHPDFVFWLVKGNEYTVLFVDPKGF
                     QNTGYQYKVDGYKDLFREKNGKHKIFKRGKFLVRVALSLYTDDSNQPPAGYREFWYDN
                     PKRIFENL"
BASE COUNT          674 a          596 c          625 g          818 t
ORIGIN      
        1 tgataaatgt gcccagatcg agcatgagtt tgtttatgtt ttgttttgac atgtgtgtct
       61 ccttgtttat tgatgccgcc attctataag cagggagctt caactgggtg gtggaaatac
      121 ggcagaaata tgaagaactt gtaaaacagt acacaaacaa cacaaaggca gtaccatcgc
      181 gaagaacgca aaattcgcaa agatgttatg gtttcctgac gctcttcgcg ggctttgcag
      241 ttcacaaccc cacagtttgg cactgcccca atatgtcact tgcaaaggtc taaaagttca
      301 accacggaga cacggagaca cagagaaaac aattaataaa gtgtctttgt ggttcaaaat
      361 ggcttttcaa agacatttct tgcagcttgt gcaagagacc aaatcaacaa aaaggcctca
      421 aaagcgtttc agcttctgaa gccttttgcg tattggtttt cgtaaaagcg gtttacttct
      481 tgagggcgtt gatctccgcc aaaaccttct taaccatttc ctcagtcaac cccgcttctt
      541 tcgcctgagg catggatagg atttgcttca aagtcatgcc tgaagccaac ccgatcaaag
      601 ggtttttgga aacaccaggc acatgctttt caagaatctt cacagcgcca gtatcatcca
      661 aaatttcgcc aacttttgtg tccaatgtat atgccatttt ttctctcctt gtgcaatgaa
      721 aatatggaat ggtcaaaacc tgcacatcta ttatacggca gggacaagga ttcagcgccc
      781 tttctcccac atctgcgcac tgatacccct gatcatagat tctcaaatat tctctttgga
      841 ttatcgtacc aaaactcacg atacccagca ggtggctggt tgctgtcatc tgtgtataga
      901 ctcaatgcca cgcgcactaa gaactttccg cgcttgaata ttttgtgttt cccgttcttt
      961 tctcggaata aatctttgta gccatccact ttgtattgat agcctgtgtt ttggaaaccc
     1021 ttgggatcaa caaataggac tgtatactca ttgcctttca cgagccaaaa gacaaagtcg
     1081 ggatggaagt cgcgcatttt atttgacgtg gggtcgtagt aagggatggt aagtttatcg
     1141 agcgtttcct cggcacggct gaacatccac caatcgtatt gattcaagag gtggttttcg
     1201 tccttgatat aggcttctaa ttggttgaca aatttcctct cgctttccac tttgataacg
     1261 cgcgagatgt aatccagtct gtcatcgttt gagacaagca agggaaggta ataatgagag
     1321 gcgatattct tgatatgcaa gcgttgacca ttatgttcaa agatttcgga tggctctgta
     1381 cgtgccaaat tcttgacagc taaggtgtat gcgtcaatat caagggttcc atcttttagt
     1441 tcttttttga gatttttctc ggcgtcagca gggtctttgt atagtctgac tgcctcgatt
     1501 ttctttttta gctcttcgag taggtctttc agtaacacgc ggatatgttt gaagtgattg
     1561 atttcatctt ccaacggttt aaagccttcc acttcctgcg ggatgagacc gaagtattgg
     1621 gttaggcgcg gaagaagaag gttgatattg ccatattttt tctgcgtatt tgggttgaaa
     1681 tagctatcag gcgatttcag ggtctcatcc agcagaccga tctgttgcgg ggtcatgcca
     1741 tgacgagcaa gcaataaacg ttcatcacca agatattcca catattgctt gagcagggcg
     1801 ctttcactgg cttggatttc aaacttgcgt ggatcgtgtt gttcgaccag taaatgacca
     1861 ttcaacttgc gatagacagg aattaagaca gggtaattgg agattgcatc tgggttgacg
     1921 cttaattcaa gttcaacacc ttcttctttt tccttctctt gtgccagccc ttcaaagatg
     1981 gtttcgaggg cttcgcgctt ggtgccgaag ataaacaggg attgcacagc ggcaaggaag
     2041 ggttttgctt gcccaaacag catgtcgtcc acttcgttgg cgttatgcag attttccagc
     2101 cgtttacgtt tgcctttcat cggttcaatg cggacgccgc gcccgacgga ttgcaataca
     2161 aatttacggg cttcactgcc tgtaccgatg ttgataaagg taatgacgtt gggacggttg
     2221 gagtcccagc cttcgtagaa gctgcgcgag cccattagca agttaatctc gctgtcttct
     2281 tcatttagac gggcaaagaa actttcatct tcaaatccct gtacgatctc atagcctgcc
     2341 agaaaatcct tcatccattc ggtggtttga ccaatcttga taagggcaaa cggtgcggca
     2401 gatgttttga gtttgaaagc gagttcttta tcattggatg ggcgaaccaa tacttcaata
     2461 tcgcccttgc tttcggcgtt gaagatgtat ttgtaaacat ccttgatgtt caggctatta
     2521 aataaggctt ggtcgagggc gaatttcttt tcttcataca gccactcagg ttcggactct
     2581 aactcggctt tcagggcttg tttggcggcg gtgaacgcct gcgtggagat ttcgcctttg
     2641 ccaatgcgtt cgagttcgcg gaagaagagt ttgaggtcgg cttcttcggt gttgacactg
     2701 ttcacgagag caa
//
LOCUS       DAOQNJ010000382         2710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28139_length_2710_cov_8.599247, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000382 DAOQNJ010000000
VERSION     DAOQNJ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28139_length_2710_cov_8.599247"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLG52_10385"
     CDS             <1..297
                     /locus_tag="PLG52_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41897.1"
                     /translation="NNDPTFELGNTCQEMQFNDDQIKEVCAAYFGDATPQMVARMKLN
                     MIMSDVGWGLWAAIQANISTIDFDFWGWALERWGRAEEKMDSKEFSVWMKDVVQ"
     gene            336..1085
                     /locus_tag="PLG52_10390"
     CDS             336..1085
                     /locus_tag="PLG52_10390"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNB41898.1"
                     /translation="MESLDLVQEMYRLVEAANAKKIQLRAIGGLAVQTHNKTDHPLFK
                     REFADLDFCIPLKQRREFEAFMPEVGYTAHRQFNLLNGDQRQIYYHNPTEMKVDIFVG
                     HFEMCHKIPLEERLNADPVTIPLAELFLSKAQIIELNRKDAFDLTSVLLNNVTGQGDN
                     ETINLKILAQLGSADWGLYKTTSINLDKVEQMLLKNEIPLNEEERAIVIGRINDIRRT
                     FAEMPKPVAWQLRDRVGTRVKWYIEVEEVQR"
     gene            1082..1255
                     /locus_tag="PLG52_10395"
     CDS             1082..1255
                     /locus_tag="PLG52_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41899.1"
                     /translation="MKQIRLVLAIILLTISIALLVWGFSPNPHETKIQMIAPTEMQLP
                     TPSSLHLNPEPVA"
     gene            1252..2028
                     /locus_tag="PLG52_10400"
     CDS             1252..2028
                     /locus_tag="PLG52_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41900.1"
                     /translation="MTHRTFSPRNLLFFLASFLAFVSIACATTAPSTSTSPTAVVDVP
                     ATATAEILIPVTGGGDEAAPALPEFRRLTLEYPPVMKVGSGSDVIILTLEVDEQGNIT
                     PTAQIDGNVTQGEVVEIPNLYATHNVTAEAYYEVSGLTVTPKGSTFQPLKQGERIRFT
                     WSVQPQEVGTYGGTIFLFLNFENRETGEKDRKEISIQIFDIKVVDFFGFSTNFVKTSG
                     VVGSVLGVIVGFPFFDDIVKYLLGRLTSEKGKPGRSKRKK"
     gene            2130..2483
                     /gene="ndhC"
                     /locus_tag="PLG52_10405"
     CDS             2130..2483
                     /gene="ndhC"
                     /locus_tag="PLG52_10405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNB41901.1"
                     /translation="MNEWLYVALFIVVGLIIPIGAIAAGFIFGPKKPNPIKESTYECG
                     IEPVGEAWIQFKAQYYIFALVFLVFDVEAVFLFPWAVKLGQLGLYAVIEGIIFVTILV
                     VGLVYTWRKGMLEWA"
BASE COUNT          682 a          611 c          675 g          742 t
ORIGIN      
        1 aacaacgacc caaccttcga gttgggcaat acttgtcagg aaatgcaatt taacgacgac
       61 cagatcaaag aagtctgtgc ggcgtatttt ggcgacgcca ctccgcaaat ggtggcgcgc
      121 atgaaactca acatgattat gtctgatgtg ggctggggtt tatgggcagc cattcaggcg
      181 aatatctcca ctattgattt tgacttttgg ggctgggcac tcgaacgctg gggcagggca
      241 gaagaaaaaa tggattcgaa agaattctca gtgtggatga aagatgttgt acagtaagtt
      301 gaaataagta aaatgagaag aagagaaata tcttgatgga atcactcgac ctagttcagg
      361 aaatgtaccg ccttgtggaa gccgcgaatg cgaaaaaaat ccaattgcga gccatcggtg
      421 ggttggcggt acaaacacac aataaaaccg atcatccact gttcaagcgt gagtttgccg
      481 acctcgattt ttgcattccg ctcaaacaac gccgcgagtt tgaagcgttc atgcctgagg
      541 tgggttacac cgcgcatagg caattcaacc tcctcaatgg cgaccaacgc cagatctatt
      601 atcacaatcc taccgaaatg aaggtggata tttttgtggg acattttgag atgtgccaca
      661 aaattccgct cgaagaacgc ctcaatgccg acccggtcac cattccgctt gccgagcttt
      721 ttctttccaa agcgcagatc atcgaactca accgcaaaga tgccttcgac ctcacatccg
      781 ttttgttgaa caacgtcact gggcaaggcg ataacgaaac gatcaacctc aagatcctag
      841 cccagcttgg cagtgccgat tggggtttgt acaagaccac gtccattaac ttggataaag
      901 tggaacaaat gttgttgaag aatgaaattc cgttgaatga agaagaacgc gccattgtga
      961 ttgggcgcat caatgatatc cgccgcacct ttgctgaaat gcccaaacct gttgcctggc
     1021 aactacgcga tagggtcggc acacgcgtca agtggtatat tgaagtggaa gaggttcagc
     1081 gatgaaacaa atccgtctcg tgcttgccat tattcttttg acgatttcca ttgcactcct
     1141 ggtgtgggga ttttctccca acccgcatga gacaaaaatt cagatgatcg ccccaacgga
     1201 aatgcaactg ccgacgcctt cttcgcttca tctcaacccg gagcctgttg catgacccat
     1261 cgaacttttt ctccccgcaa ccttttgttc tttttggcga gcttccttgc ttttgtaagc
     1321 attgcttgtg ctacaactgc tccgtccact tccacttctc ccaccgccgt tgtggatgtt
     1381 ccagcgactg ctactgccga aattctcatc ccagtgaccg gcggaggaga tgaggcggcg
     1441 ccagccctcc cagaatttcg ccgcctaact ttggaatatc cgcccgtgat gaaggtcggc
     1501 tcaggcagcg atgtcatcat cctaaccttg gaagtggatg aacaggggaa tatcacccca
     1561 actgctcaga tcgatgggaa tgtaacgcaa ggggaggtcg ttgaaattcc aaatctttat
     1621 gcaacccata atgtgacggc tgaagcctat tacgaagttt cagggttgac ggtgacgccg
     1681 aaaggctcta cttttcaacc gttgaaacaa ggggagcgca ttcgttttac atggagtgtg
     1741 caacctcaag aagtggggac atacggcggc acgattttcc ttttcttgaa ttttgaaaac
     1801 cgcgagactg gcgaaaaaga ccgcaaagaa atttccatcc aaatttttga catcaaagtg
     1861 gtggactttt ttggcttttc tacgaatttt gtgaagacct ctggtgtggt cggctcggtt
     1921 ttgggtgtga ttgttggctt tccatttttt gacgatatcg tcaagtacct gctcgggcgt
     1981 ttgacttcag aaaaaggcaa gccgggcagg tctaaaagga aaaagtgaca aatatcaccc
     2041 aaaaaattga caaccattaa tggggggcta atttgttctt tatggtacaa tgcgcaagtt
     2101 gtaatacatt tcccaatgga gcgaattaaa tgaatgagtg gttgtatgtc gccctgttta
     2161 ttgttgtggg tctgatcatc ccaattggtg cgatcgccgc tggctttatt tttggcccca
     2221 agaagccgaa ccccatcaaa gaatccacat atgaatgtgg tattgaacct gttggtgaag
     2281 cttggattca gttcaaggcg caatactata tttttgcgtt ggtctttttg gttttcgatg
     2341 tagaagcggt tttccttttc ccctgggcgg tgaaactcgg tcagcttggt ttgtatgctg
     2401 tgatcgaagg tatcatcttt gtcaccattt tggtcgttgg tttggtgtac acatggcgca
     2461 agggcatgtt ggagtgggcg taaatacacg gtaaattaaa gagaaggctg atgggatttt
     2521 tcctctcagc cttttctttg tgtaacagga gtgatttatg agtttctgga atgaccccct
     2581 gagagttgct gccgagtggc ttgagggcat attgaccggc tggggcttga atgccattgc
     2641 cgcacatgtg cttgtggttt tcctcggtgt attcctgctc atcacattgt tgatggtgtt
     2701 ggatatcttc
//
LOCUS       DAOQNJ010000383         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28233_length_2705_cov_7.345660, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000383 DAOQNJ010000000
VERSION     DAOQNJ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28233_length_2705_cov_7.345660"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLG52_10410"
     CDS             <1..314
                     /locus_tag="PLG52_10410"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNB41902.1"
                     /translation="ILPVGVAQAGAWKYSHIYTITTSHFPELAERARTITESQARAKL
                     LELYFEMVGAAQIRDVQKLFGWGNEVVKRVVGKLVDAGGLVLAEHPKQTGEWVAVKEA
                     I"
     gene            416..1105
                     /locus_tag="PLG52_10415"
     CDS             416..1105
                     /locus_tag="PLG52_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB41903.1"
                     /translation="MVRPSKKQQLPNLQEAIKETAWKQIAEKGAAALSLRAIARELKI
                     TAPAIYNYFPDRDALVTALIIDAYTSFGDSQIAARDAISKEKIEERFTAVGIAYRQWA
                     KTYPQRYQLIFGTPIPGYQAPLMEILPSAARSLSASVSVIDDLRIVNRLNSSGYPNVQ
                     PGYEQQFEVWKKFAGDYDILSLAVSMLVWSRVHGLVSLEIANNMPPFGVDGDSLYMYE
                     LKSIIKQFIKP"
     gene            1157..1597
                     /locus_tag="PLG52_10420"
     CDS             1157..1597
                     /locus_tag="PLG52_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41904.1"
                     /translation="MYTVSITRLRVRSIFFMPLFTYHAMRTSMQVQKSEGIVGADTRF
                     EKNNVVWTKTVWTEESAMKKYRGSGAHQIAMRILSEMCSEASVTRWQQESTELPTWEE
                     AHRRMLTEGKQSKVKHPSALQASGKTAPDTMQSGFKMPTPPVTQ"
     gene            1594..2445
                     /locus_tag="PLG52_10425"
     CDS             1594..2445
                     /locus_tag="PLG52_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB41905.1"
                     /translation="MSLAKKWMFNHPVTHRVKDGYFENDGIRQHYVTLGEGKLVVLIH
                     GIPEFWYSWRHQIDALSQQYQVVAIDQRGFNESGKPASTDGYRVERLVEDVAALIRYL
                     GRKQAVIVGHDAGAWLAWHFAALHPELTERLVILSVPHPNAMREELAKHGEQHRASEY
                     ARTMQKEGAKPMFRTWQISLLLDRLAFPLYLSADRGTDADAITSFYQFNYPQEPYTLD
                     ESLPIIEAPTLVIHGKRDRFLIASGHEHNARWLKHEPKTVMLDADHFVHQEKPDEVNR
                     IILDWLA"
     gene            2447..>2705
                     /locus_tag="PLG52_10430"
     CDS             2447..>2705
                     /locus_tag="PLG52_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB41906.1"
                     /translation="MEIIKIKLSVSNAYLVKDRKSILVDTGSSHEADKILAEVKRAGV
                     NEKDISLILHTHGHVDHAGSTAELKRRLGVPVAVHAEDAFML"
BASE COUNT          708 a          603 c          718 g          676 t
ORIGIN      
        1 aaattttacc ggttggcgtt gctcaagccg gtgcttggaa gtattctcat atctacacca
       61 tcacgaccag tcatttccct gaattggcag aacgagcgcg gacaatcacc gaatcgcaag
      121 cgcgggcaaa gttattggaa ctatactttg agatggtcgg ggcagcgcaa atccgcgatg
      181 tgcaaaagtt gtttggttgg gggaatgagg tggtgaagag ggtagtgggt aaactggtgg
      241 acgcgggcgg tttggttctg gcggagcatc ccaaacaaac tggagaatgg gtggcagtaa
      301 aagaagcgat atagcaaacc tcggaggttt tcaaagcctc cgaggttttt ttttaccctt
      361 gacttaattt actatacact gtatatttac actgtatagt aaataaggag ccgaaatggt
      421 cagaccgagc aagaagcaac aactcccaaa ccttcaagaa gccatcaaag aaactgcgtg
      481 gaagcagatt gctgaaaagg gggcggcggc attatcccta cgggcgattg cacgcgaact
      541 gaagatcaca gcgccggcta tctacaacta cttcccagat cgggatgcgc tggtgacggc
      601 actcattatt gatgcctaca cttcctttgg cgactctcag attgctgcta gagatgccat
      661 ctcaaaagaa aagatagaag agcgatttac tgcggttggc attgcctacc gccagtgggc
      721 gaagacctat ccgcagcggt atcagcttat ttttggcacg cctattccgg gatatcaagc
      781 tccattgatg gagatcctac cgtccgcagc gcgttctttg agcgcctctg tgagcgtcat
      841 tgatgatttg cgtatagtca ataggttaaa ttcgagcgga tatccaaatg ttcagcccgg
      901 ctatgagcag caatttgaag tgtggaaaaa atttgcaggg gattacgaca ttttgtcgtt
      961 ggctgtctct atgttggtgt ggagtcgtgt tcacggcttg gtttctttgg agatcgcgaa
     1021 caacatgccg ccctttggtg tggatggaga ctcgctttac atgtacgaac tgaaatccat
     1081 cattaagcaa tttatcaaac cgtaatattc aaaaatatcg ccttacctac acaaccaaat
     1141 aaatatagga gtcatcatgt atacagtttc cattacccgc ttgcgagtcc gctcgatctt
     1201 ttttatgcca ctatttacat atcatgccat gcgtaccagc atgcaggtac aaaaatctga
     1261 agggattgtc ggcgcagata ctcgctttga aaaaaataac gttgtttgga caaagaccgt
     1321 ctggacggaa gaatcagcga tgaaaaaata tcgtggttca ggcgcgcatc agatcgccat
     1381 gcgtattctg tctgaaatgt gttctgaagc ttcagtgacc cgctggcagc aagaaagcac
     1441 ggaactgccc acatgggaag aagcgcatcg ccgcatgttg accgagggta aacagtctaa
     1501 ggtgaaacat ccgtcagcgt tgcaggcttc cgggaagacc gctccagaca ccatgcaatc
     1561 gggtttcaaa atgcctactc cgccggtgac ccaatgagtc ttgcaaaaaa atggatgttc
     1621 aatcaccctg tcactcatcg cgtgaaggac ggatactttg agaacgatgg tattcgtcag
     1681 cattatgtca cattaggtga aggcaagttg gtggtgctca ttcacggcat ccctgagttt
     1741 tggtattcat ggcggcatca gatagatgcg ttgtcgcagc agtatcaggt tgtcgcaatc
     1801 gaccaacgcg gattcaacga gtcgggtaaa cctgcttcca cagacggcta tcgggtggag
     1861 aggttggtgg aagatgttgc agcgttgatt cggtatctcg gacggaagca ggcggtgatc
     1921 gttggtcatg atgcgggggc gtggctggca tggcatttcg cagcgcttca tcccgaactg
     1981 acggagcggc ttgtcatctt gagcgttccc catccgaatg cgatgcgtga agaattggcg
     2041 aagcatggtg agcaacaccg tgccagtgaa tatgcccgca caatgcaaaa ggaaggtgca
     2101 aagccgatgt ttcgtacctg gcagatcagc ttgctgttag accggctcgc gttccccctg
     2161 tatttgtctg ctgatcgtgg aacggatgcc gacgcgatta cttcgttcta tcaattcaat
     2221 tatccgcaag agccgtatac gctggatgaa agcctgccga ttatcgaagc accgaccttg
     2281 gtcattcacg ggaaacgtga ccgtttcttg atcgcttcag ggcacgaaca taatgcacgc
     2341 tggctcaagc acgaaccaaa gaccgtgatg ttggatgcag atcattttgt gcatcaggaa
     2401 aagcctgatg aggtcaatag aatcattttg gattggcttg cataacatgg aaatcatcaa
     2461 gataaagctt agtgtgtcaa atgcttatct ggttaaggat agaaaatcga tccttgtcga
     2521 tactggttcc agccatgaag cagataagat acttgctgaa gttaagcgtg caggtgtgaa
     2581 cgaaaaagat atttccctta ttttgcatac ccatgggcat gtagatcatg ccggttcgac
     2641 ggcggaactc aagcgcaggc tgggcgtgcc ggttgctgtc catgcagagg atgcgttcat
     2701 gttga
//
LOCUS       DAOQNJ010000384         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28285_length_2702_cov_6.508878, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000384 DAOQNJ010000000
VERSION     DAOQNJ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28285_length_2702_cov_6.508878"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1549)
                     /locus_tag="PLG52_10435"
     CDS             complement(149..1549)
                     /locus_tag="PLG52_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB41907.1"
                     /translation="MLSSLRSRLWLSYTFLIVTALSVVTIVLFASLFRNPLLYRQTTE
                     RLNAVRSVMVTRLSEADAPPISTLAMRNAQTFNVRVAVFSQNRELVFDTSTEAELIFP
                     TVKRLTRALPLTRDTNGAAWLYSVEKLPDGTFLMVATPRPRNSVLNIFSDEFIPVIVL
                     GGGSALLLSLILAFLVSGWIANPLQKVVNAARGMPSAEVKPLEATGPHEVQELTRAFN
                     SMIARMQASQRSQREFVANVSHEMKTPLTSIQGFAQALLDGTANTQEPQQKAAQVIYN
                     ESERMHRMVLDLLDLAKLDAGTADLKMAPLNIRALLISIQEKFIPQSQRAGVKIETEI
                     PVGLPPFIADGDRMAQVFTNLTDNALKFTPRGGTVSIAAFFALDTMHVTVADTGVGIR
                     EEDLQNIFQRFYQADPARKGGEAHGAGLGLAIAQEIVAAHGGKITVRSRIGAGTTMEV
                     ILPLKPPDATKLVRKK"
     gene            complement(1585..2265)
                     /locus_tag="PLG52_10440"
     CDS             complement(1585..2265)
                     /locus_tag="PLG52_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB41908.1"
                     /translation="MSAELILLVDDEPSITELARMYLERDGYRIHEAADGESALETAE
                     KQKPALIVLDVMLPKVDGFDVCRRLRSAGNDVPIIMLTARDEDIDKILGLELGADDYL
                     TKPFNPRELVARVKAILRRSESKKDSDGKPIHRGDLTIDPVSREARLASRTLDLRAQE
                     FDLLLTLAESPGRVFSREQLLQLAWGFDYYGQTRTVDVHIAHLRKKLEGGNIKIETVT
                     GVGYKFVT"
     gene            complement(2266..2646)
                     /locus_tag="PLG52_10445"
     CDS             complement(2266..2646)
                     /locus_tag="PLG52_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB41909.1"
                     /translation="MSKQVIHTEKAPKAIGPYSQAIHIGNLVYTAGQVALDPATMELV
                     AGGVEEQTRQVLANLTNVLEAAGTSMSKVVKTTVFLKTMDDFTKMNAVYAEIFGENPP
                     ARTTVAVSGLPKNALVEIECVALA"
BASE COUNT          688 a          673 c          661 g          680 t
ORIGIN      
        1 gataggtctg tgaacgcaaa gcctccaaca atttgtggat ggtggcttct tcgttataac
       61 aggggatgat cacagaaacg gtaggttggt caggcatggt tctggcaaga agtcgccgcc
      121 agtgagaggc ggcgatgagt tttcaggttt actttttacg tacaagcttt gtcgcatctg
      181 gcggtttcag tggaaggatg acttccattg ttgtgccagc ccctatacga ctacggactg
      241 taattttacc accatgcgcc gccacgatct cctgcgcaat tgccaacccc aagcctgctc
      301 catgtgcctc tccgcccttg cgtgcggggt ctgcctgata aaacctttga aagatatttt
      361 gcaaatcttc ttcacggatt cctacacccg tatccgctac agtgacatgc atggtatcta
      421 gtgcaaaaaa agcagcgatc gaaacagtcc cgcctcgtgg agtgaatttg agggcattat
      481 ctgtcaagtt cgtgaatact tgtgccatac ggtcgccgtc ggcgatgaaa ggcggcagac
      541 cgactggaat ttcagtttcg attttcacac ccgcacgttg agactggggt ataaactttt
      601 cttgaatcga aattaacaaa gcccggatgt ttaaaggtgc cattttcagg tcggctgtgc
      661 ctgcgtctaa tttggcaagg tcgagcaagt ccaacaccat acgatgcatc cgctcggatt
      721 cgttataaat cacctgtgcg gctttttgct gcggctcttg cgtgtttgcc gtcccatcga
      781 gcaaagcttg ggcaaatccc tgaatggatg taagcggcgt tttcatttca tgcgagacgt
      841 tcgccacaaa ctcgcgctgt gaccgttgac ttgcttgcat gcgagctatc atggaattaa
      901 atgcccgcgt caattcttgg acctcgtgcg gacccgttgc ttcgagcggc tttacctccg
      961 ccgaaggcat tccccgcgcc gcgttgacaa ctttctgcag cgggttggcg atccacccag
     1021 agacgaggaa cgccagaatc agcgagagca gtaaagcgct cccaccgccc aaaacaataa
     1081 cggggataaa ttcatcacta aagatattaa gaacagagtt acgtggacgg ggcgttgcta
     1141 ccatgagaaa cgtcccatcg ggtagctttt caacggaata caaccatgcc gccccattgg
     1201 tatcacgggt caatggcaag gcacgcgtta accgcttaac ggttgggaaa ataagctctg
     1261 cttctgtgct ggtatcaaac acaagttcac ggttctggga aaacacagcc acacggacat
     1321 taaacgtctg cgcgttacgc attgccaatg tagaaattgg cggtgcatcc gcttcgctta
     1381 aacgcgtcac cattacactg cgcactgcgt tcaagcgctc tgtcgtctgc cgatataaaa
     1441 gcggatttcg aaacagcgag gcaaatagaa caatcgtcac aacactcaaa gctgtgacga
     1501 tgagaaaggt gtagctaagc caaaggcgag atcgaagaga agagagcata aaagttgagt
     1561 tttacaaatt acgcaacgcg tatcttaagt gacaaatttg taccctactc ctgtgacggt
     1621 ttcaatcttg atattgccac cttcgagttt cttgcgaagg tgtgcaatgt gaacatccac
     1681 ggtgcgggtc tgaccataat agtcaaagcc ccaggcgagt tgcagtaatt gttcacgcga
     1741 gaagacacgc cccggcgact ctgccagagt gagcaaaagg tcaaattctt gggcgcgcag
     1801 gtctaaggtc cgcgaagcga ggcgggcttc tcgagaaaca gggtcaatcg tcaagtcacc
     1861 gcggtggatc ggtttaccgt ccgaatcctt tttggattca ctacggcgca aaatggcttt
     1921 aacacgcgcc accaactctc gtgggttgaa cggtttggta aggtaatcat ctgcgccaag
     1981 ttcaagccca agaattttgt cgatatcttc atcccgagcg gtgagcataa tgatgggaac
     2041 atcgttcccc gcagaacgta atcgacgaca gacatcaaag ccatctactt tggggagcat
     2101 cacatccaat acaataagag ccggcttttg tttctctgcc gtctctaacg cagactcgcc
     2161 atcggcggct tcgtgaatgc gatatccatc acgctcaaga tacatgcgcg caagttcagt
     2221 aatggagggc tcgtcatcga cgagtagaat taattcagca gacatttatg ctagggcaac
     2281 gcactcgatc tcaaccaagg cattctttgg taaaccagaa actgctacgg tagtacgagc
     2341 aggtgggttt tcaccaaaga tttctgcata gacagcattc atttttgtaa aatcatccat
     2401 agtcttaaga aagaccgtag tctttaccac tttgctcata cttgtaccag cggcttccaa
     2461 tacattggtg aggttggcta acacttggcg agtctgttct tccacaccac cagcgaccag
     2521 ttccatcgta gcagggtcaa gcgcaacctg ccccgctgtg tataccaaat tgccaatatg
     2581 aatagcttgc gaataaggtc caatcgcttt gggggccttt tcagtgtgaa taacttgttt
     2641 gctcatgttt tcctcgtttt actgaagtta attgttttct ttattttaat cgaggaaagg
     2701 ca
//
LOCUS       DAOQNJ010000385         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28390_length_2696_cov_9.173419, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000385 DAOQNJ010000000
VERSION     DAOQNJ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28390_length_2696_cov_9.173419"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PLG52_10450"
     CDS             <1..974
                     /locus_tag="PLG52_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNB41910.1"
                     /translation="LRYQQMFEDVGVTKGRVGVYGHYDVGAVFGWLNHLQKLMPAIEF
                     VGEARDDSIFLRAMETKDENEVARIRKMGKVTTAVVEKVKEYLTSCDVREDEVLLKED
                     GTPLSVGDVHSKIRLWVSEHGAELPSGFIFAIGRDAGVPHSAGNPADLMRLGQTIVFD
                     IYPAEAGGGYFYDFTRTWSLGYTTPEAQSLFDQVKEIYDTLNDNFDLNVAFKDYQRMT
                     CEFFEGKGHQTPLNTKAPIEGYVHSLGHGVGLNIHERPFSGLTAGDDQRLRPGVIITS
                     EPGLYYPEKGMGFRIEDTLWVRPDGVMEKLADYPYDFVLPMKKWKKK"
     gene            1005..1838
                     /locus_tag="PLG52_10455"
     CDS             1005..1838
                     /locus_tag="PLG52_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB41911.1"
                     /translation="MSIVNRLLSIMHLQSLTPRPFLDSDLNEFPFTVPVIRSLPEIKF
                     TSPVTFFVGENGSGKSTVLEALACAVESITVGTESVKTDKTLAPVRKLSKYFKLAWTK
                     RTRKGFFMRAEDFFGYARSMRETREELEEEYNNVERDYEGRSKYAADLARSAYAGQLA
                     AMKNRYGRDLDNFSHGEAFLTLFQARFVPGGLYLLDEPEAALSPSRQLSFLSALRQMV
                     EEEAQFIIATHSPIILAYPGAQIFSFQNGAIKQVQYEELEHVQLTKDFLNNPQSYLRH
                     L"
     gene            1847..>2696
                     /locus_tag="PLG52_10460"
     CDS             1847..>2696
                     /locus_tag="PLG52_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNB41912.1"
                     /translation="MISMLRSRTFLYFLIGISLLIALVFGGYYLYNTYRVRTVVIATN
                     EEGSQSYIMMDALAKAVQQRDSRIKVKIVQTAGSADIMKLLESHQADFGTAQLDAELP
                     DQVQTVALLYPQAFHMIVPAASDIWSPADLRGKKIATSSKSGGGYKSVFELLAYYGLS
                     PEDVEVIPFEDGDLRDAAFINGDVDAMFRANTIGSSSLRELAAQKDIRFLQLEQFDAM
                     RIQYPYLFQYVIPKGTYRADAPIIPVQDTKVVGVPTLLFVNANVSEDIVHLFASALFE
                     SQNDIVQ"
BASE COUNT          663 a          586 c          706 g          741 t
ORIGIN      
        1 ctttgcggta tcaacagatg tttgaagatg ttggggtgac aaagggacgt gttggtgtat
       61 atggtcatta tgatgttggt gcggtgtttg ggtggctgaa ccatcttcag aagttgatgc
      121 ccgcgattga gtttgtcggt gaagcacgtg atgactctat ctttttgcgc gccatggaaa
      181 ccaaggatga gaatgaagtc gcgcgtatcc gcaaaatggg taaggtaacg actgcagtcg
      241 tagagaaggt aaaggaatac cttacctctt gcgatgtacg cgaggatgag gtgttactca
      301 aggaagatgg cactccgctt agcgtaggag acgtccactc gaagatacgg ctttgggttt
      361 ctgaacatgg cgcagagttg ccttcgggtt ttatctttgc cattggtcgc gacgccggtg
      421 tgccgcactc cgcgggcaac cccgctgacc tgatgcgttt gggtcagacg attgtttttg
      481 atatttatcc ggcagaagca ggtggtggct atttctatga tttcacccgt acgtggagcc
      541 ttggctatac tacgcctgaa gcgcaatcat tgtttgacca agtcaaagaa atctacgata
      601 cgctcaacga taattttgac ttaaatgtgg cattcaagga ttatcaacgc atgacctgcg
      661 aattctttga aggcaaaggg catcaaactc cgctcaatac caaagcgcca attgagggat
      721 atgtccacag ccttgggcat ggtgttgggt tgaacatcca cgaacgccca ttcagtgggc
      781 tcacggcggg ggacgatcaa cgcctcagac ccggagtgat cattacctcc gaaccgggct
      841 tgtactaccc cgaaaaaggg atgggcttcc gtatcgaaga tacgctttgg gttcgccctg
      901 atggcgtcat ggaaaagctg gcagattacc cgtatgattt tgttttgcca atgaagaagt
      961 ggaaaaagaa ataatgtcga cgattgacga tggaccatga aagaatgtcc atcgtcaatc
     1021 gtctattgtc catcatgcac cttcaatcgc ttacacctcg tccatttctc gatagtgacc
     1081 tgaatgaatt tccgttcacg gtgccggtga ttcgttcttt gccagagatc aagttcacct
     1141 caccggtaac cttttttgtg ggggagaacg gctcgggcaa atcgacggtg ttagaggcat
     1201 tggcttgtgc ggtagagtcc attacggtgg ggacggaaag cgtcaagacc gataaaaccc
     1261 ttgcgcctgt tcgtaagcta tcaaagtatt tcaaacttgc atggacgaaa cgtacgcgca
     1321 agggcttttt tatgcgcgcc gaagattttt tcggatatgc ccggtccatg cgagagacgc
     1381 gtgaagaact ggaagaagaa tacaacaatg tagagcgcga ttatgaagga cgctcgaaat
     1441 atgctgctga cttggcacgt tcggcgtatg ccggtcaact tgcggcgatg aagaaccgtt
     1501 acggtagaga cttggacaac ttctcgcacg gcgaggcatt ccttactttg tttcaggcac
     1561 gttttgtccc cggcggctta tatctgttgg atgaacccga ggctgcgctt tcgccgtcca
     1621 gacagcttag cttcctttcg gcactcaggc aaatggtcga agaagaggcg cagtttatca
     1681 tcgctactca ttcgccgatc attttggcgt atccgggggc gcaaatattc agctttcaaa
     1741 atggggctat aaagcaagta caatacgagg agcttgaaca cgtccaactt accaaggatt
     1801 ttttgaataa cccgcaatct tacttgcggc atttatagat acaggaatga tctcaatgct
     1861 tcgctcgcgc acttttcttt atttcttgat cggcatttcc ttattgatcg cattggtgtt
     1921 tggtggatat tacttgtaca acacgtaccg tgttcgtacg gtggttattg cgacaaatga
     1981 agaaggcagc cagagttaca tcatgatgga tgctttggcg aaggcggttc aacagcgtga
     2041 ttctcgtatc aaggtaaaga ttgttcaaac cgccgggtct gccgacatta tgaagttgct
     2101 cgagtcacat caagcagact ttggtaccgc gcaactggat gctgaactac cagatcaggt
     2161 ccaaaccgtg gcgttgttgt acccgcaggc gtttcatatg atcgtgcctg cggcttcaga
     2221 tatctggagc ccagctgatt tgcgtggaaa aaagatcgca acatcttcta aaagtggcgg
     2281 cggttataaa tctgtctttg agttactggc ttattatgga ttatctcctg aagatgttga
     2341 ggtgattcca tttgaagatg gcgatttgcg ggatgctgct tttataaatg gggatgttga
     2401 tgccatgttc cgtgcaaata cgattggtag cagcagtctg cgcgaattgg ctgcacagaa
     2461 ggacattcgt tttttacaac tggaacagtt tgatgcaatg cgcattcaat atccgtattt
     2521 gtttcagtat gtaatcccaa aagggaccta ccgcgcagac gctcctatca tccctgttca
     2581 agatacaaaa gttgttggtg tgccaaccct cttgtttgtg aatgccaatg tgtctgaaga
     2641 cattgtgcat ttatttgcct ctgctttgtt tgaaagccag aatgatattg ttcaaa
//
LOCUS       DAOQNJ010000386         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28391_length_2696_cov_7.554714, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000386 DAOQNJ010000000
VERSION     DAOQNJ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28391_length_2696_cov_7.554714"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1074)
                     /locus_tag="PLG52_10465"
     CDS             complement(235..1074)
                     /locus_tag="PLG52_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNB41913.1"
                     /translation="MPIELTTLSQSSLQDYNDCPRRFELRYLQHLEYPAIETEPALEN
                     EKHQREGEYFHRLVQQYFIGIPEKQLAKTANTDNLERWWNNFIGDTDLRGLKDLGGLR
                     TEVTLSAPLGSFRLIAKYDLIANDGKKFYIYDWKTYRKRPKNEFLAIRLQTRVYRALL
                     VQAGAFLNGGKPIAPEQIEMVYWFSDFPNAPARFPYQADQFKRDWDAITRYAEEITLR
                     QAQGGASSYPKTEDVNRCLYCPYRSYCNRGVHAGDALDVELEAEAEELFDINFEQIGE
                     IEF"
     gene            1180..2301
                     /locus_tag="PLG52_10470"
     CDS             1180..2301
                     /locus_tag="PLG52_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNB41914.1"
                     /translation="MGQETQVAVFIDYDNIEISVEEAFGKGTDVDWSRVFNYASQIGR
                     VVLRRAYADWAEAAAKQRQLLNMGVELIHVNSKRGKNAADIRIVLDAMELFYNGRDSF
                     THVLLASGDGDFTELVHRLRANGKTVIGLGISGTTADYLVSACDKFVYYDKWQGVGKF
                     KKETPSNGGQKQQPVKQSTPPAQKQEKHPPQEKPQVQEKAQPQEKSAPKPATPISPEK
                     RLERYLNILSSHKIRMVPSTNRPLVIYKIYDIVKEDAGITFNQLKEKAQAVFANTKVE
                     PQLALDTAHQLFHTFCFEFDQSNDRIMDRKMLIPEDTKSPADLLNKCDQKLLHLIAGD
                     LGASEVFDKEIAAKILYGGVRNPKVLDHIQELLDAETQV"
BASE COUNT          697 a          652 c          667 g          680 t
ORIGIN      
        1 gcgcgccaga caatcgataa ttcgctgcca gtagctcaag gtcgaggacg ttgatcacgc
       61 cgtcgacctt gagctactgg cagcgaatta tcgattgtct ggcgcgcagc cgacctcaac
      121 aacgacggcg tgatcaacgt cctcgacctt gagctactgg cagcgaacta tcgcttatct
      181 ggcgcacttg cgtggtaaca aaaaaggcga cccgtcaggg tcgccttttt tgttttaaaa
      241 ctcgatctcc ccaatctgtt caaagttgat atcaaacaac tcttcggctt ctgcctctaa
      301 ttccacatca agcgcatctc cggcatggac tccgcggttg cagtatgatc tataagggca
      361 gtaaaggcag cggttgacat cttcagtctt cgggtaggaa gaggcgccgc cctgagcctg
      421 tcgaagggtt atttcctctg catagcgtgt gatggcatcc caatctcgtt tgaactgatc
      481 ggcttggtat gggaatcgag ccggggcgtt gggaaaatcg gagaaccaat acaccatctc
      541 gatctgttcg ggggcaatag gcttgccacc gttgagaaac gcgcccgctt gcactaacaa
      601 cgcgcggtag acgcgagttt gcaaacggat ggcaagaaac tcattcttgg ggcgtttgcg
      661 ataggtcttc caatcgtaga tgtaaaactt ttttccatcg ttggcgatta gatcgtattt
      721 ggcgatgagg cggaatgaac ctaatggggc tgataaagtt acctcagtcc ttagacctcc
      781 gaggtcttta agacctcgga ggtctgtatc acctataaaa ttgttccacc atctttcaag
      841 gttatctgtg ttagctgttt tcgcaagttg tttttctgga atgccgatga aatactgttg
      901 caccagccga tgaaaatatt caccttcgcg ctggtgtttc tcgttttcaa gtgcgggctc
      961 ggtttcaatg gcgggatact ctaaatgctg aagataacgc agctcaaaac ggcgcggaca
     1021 gtcgttatag tcttgcaggg acgattggct gagcgtagtc agttcgatgg gcatgtctta
     1081 ttttgccaca gaattttgtg cctatgctat acttttgcca tactacgggg atggataggg
     1141 agaaaaacct taccatcttc atgaaaattt cggagtttta tgggacagga aacacaagta
     1201 gccgttttta tcgactacga caatattgaa ataagtgtgg aagaagcctt tggcaagggg
     1261 accgatgtag attggagtcg tgttttcaac tacgcgtcgc agatcgggcg cgtggtttta
     1321 cgtcgcgcct atgccgactg ggcagaagcc gccgccaaac aacgtcaact gctcaacatg
     1381 ggcgtagagt tgatccatgt caacagcaag cgcggcaaga acgccgccga tattcgcatc
     1441 gtgctcgatg ccatggagct tttttacaac ggacgtgatt ccttcacgca tgttttgctt
     1501 gcttcaggag atggtgattt tacagagttg gtccatcgtt tacgcgccaa tggtaagacc
     1561 gttattggtt tgggcatcag cggcaccact gcagattatc tcgtcagcgc ttgtgacaag
     1621 ttcgtgtact acgataaatg gcagggtgta ggcaaattca agaaggaaac gccttcgaac
     1681 ggcgggcaga aacagcagcc agttaagcaa agcactccgc ctgcgcagaa acaggaaaaa
     1741 catccaccgc aggagaagcc tcaagtccaa gaaaaggcgc aacctcaaga gaagtccgcg
     1801 ccgaagcccg ccacaccgat ctcaccagaa aaacgcctgg agcgttatct caatattctt
     1861 tcttcgcaca agatccgcat ggtgccaagc acgaaccgtc cgctggtgat ttataagatt
     1921 tacgatatcg tcaaagaaga cgcgggcatt accttcaacc aactaaaaga aaaagcacaa
     1981 gcagtttttg cgaatacaaa ggtcgagccg caactcgcgt tggatacggc gcatcagctt
     2041 ttccatacct tctgttttga gtttgaccaa agcaacgacc gcattatgga tcgcaagatg
     2101 ttgattcctg aagatacaaa atcacctgct gacctgctca acaaatgtga ccagaagttg
     2161 cttcacttaa ttgctggcga ccttggtgct tcggaagttt ttgataaaga gatcgctgcc
     2221 aagattttgt atggcggtgt acgcaacccc aaggtgcttg atcatattca agaattgctc
     2281 gacgcagaaa ctcaggtata atcgcaaata tgctttacat catcagcgta ggcaaacccc
     2341 tttttgcaat ggcgatcaat ggctttgata acgccgttcc ttgtcgtatt gcgcctgtac
     2401 gctgaaagtt tttactttca tacgccgcag gcattgcctg cggcgttttt gtttaataat
     2461 atcgtaaggg caacccgcct agattgatca aacactttga caaaccttga agggtcgccc
     2521 ctacaaaccg gaaaaaacta tgatccaaat tcaactctcc aacatcacac tcgtcctcgg
     2581 tgccaagcgc atctttgaaa atctcaactg ggagatccag cgcggacagc gtattggtct
     2641 tatcggtgcg aatggcgcgg gcaagtcgtc gctattcaaa cttattgaag gggaac
//
LOCUS       DAOQNJ010000387         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28450_length_2693_cov_9.148976, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000387 DAOQNJ010000000
VERSION     DAOQNJ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28450_length_2693_cov_9.148976"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLG52_10475"
     CDS             <1..814
                     /locus_tag="PLG52_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HNB41915.1"
                     /translation="LAEDRFFIVTGTAFGQHDMSWLSLQMPEDGSVTIEDVGSNYACI
                     GMWGPKARTILEKVTTDDVSNAGFPYMTSKNINVGDVPVRASRVTYVGELGWEFYCPM
                     EYGLRLWDTLWEAGQSEGMVAAGYKAIESLRLEKAYRYWSGEISPDYTPYEAGLGFAV
                     KLDKENFLGKEALVKQKAEGVKTKLCTMTLADDRTIVMGKEPIRIDGKIVGWVASGGF
                     GYSVAKSIAYAYLPLEYSKPGTKLEVECFGEQVGAEVTANVLWDPKNERIKA"
     gene            939..1874
                     /locus_tag="PLG52_10480"
     CDS             939..1874
                     /locus_tag="PLG52_10480"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HNB41916.1"
                     /translation="MNTLAIDKAIAKVPFLADSKNIKKIPLSGGITNLNFKIEADGRS
                     YVIRLAGEGTDQLGIKRDVEYAANKVAGELGIAPEVVYFIEPEGYIVTRFVTGKRIMP
                     DEIVKPDYLTRVAQKLRLFHRNAPKLKGEFNVFRRVEMLTKTAKAKGAHFPEDWAFIQ
                     KKMREAEKALLKDPYKPTPCHNDLLNLNWLDEEVAGDIGEIRLLDWEYAGMGDIFFDL
                     GNFSHHHRLNEDQIRIFLTAYFGKFTPKHYARLRIMWAMSELHEAMWGTTQSVISTLE
                     EDFQGYANLWFGRYRMHVTDYRWEQWLKDASKKGK"
     gene            1989..>2693
                     /locus_tag="PLG52_10485"
     CDS             1989..>2693
                     /locus_tag="PLG52_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNB41917.1"
                     /translation="MSIVNAKEIMIPAAKEGWAVGAFNVTDLLQFEAVIDAAIEKKAP
                     VIVQTSVKPSQFLGTNMMVAIYRTLAESAPVPVCLHLDHSTDINYCKKCADAGYTNIM
                     IDASKQSYEENIRQTKEVVDYAHSVGNISVEGELGTVGGVEDQIKVAEDEAQLANPEQ
                     SIEFVERTGVDIFAPAIGTAHGVYKTKNPKIDFERMATIHKMLNSNGIKTPLVVHGGT
                     GLPDDYVAIAVQHLVNG"
BASE COUNT          720 a          647 c          704 g          622 t
ORIGIN      
        1 cctcgcagaa gaccgcttct tcattgttac aggaactgcc ttcggtcaac acgatatgtc
       61 ttggctttcg ttacagatgc ccgaagatgg ttcagtgacg attgaagatg tcggctcaaa
      121 ctacgcctgt atcggcatgt gggggcccaa ggctcgcact atccttgaga aagtcactac
      181 agatgatgtc tctaacgcgg gcttcccgta catgacctcg aagaatatca atgtaggcga
      241 tgtgcccgtc cgcgcctcac gtgtgaccta tgtcggcgaa ctcggctggg aattctactg
      301 cccaatggaa tacggtctgc gcctgtggga tacgctctgg gaagctggtc aatctgaagg
      361 aatggtggca gctggttaca aagccattga gtcgctacgc cttgagaaag cctatcgcta
      421 ctggagcggc gaaatttcgc ccgattacac tccttacgaa gcgggtcttg gctttgctgt
      481 aaaacttgat aaagaaaact tcctcgggaa ggaagctttg gtgaagcaga aggcagaagg
      541 agtcaaaacc aaactttgca cgatgactct cgccgatgac cgtaccattg tgatgggcaa
      601 ggaaccgatt cgtattgatg gcaagatcgt gggctgggtc gcttcaggcg gatttggcta
      661 ctcggttgcg aaatcgattg cgtatgccta cctgccgttg gaatattcca agccaggcac
      721 caaacttgag gttgaatgct ttggcgaaca ggtcggtgcg gaagtcacag ccaatgtgct
      781 gtgggacccg aagaacgaac gaattaaggc gtagttcgag agaatatctc gaactacaga
      841 aaaggctcga gaaagcagaa gcagatttca gttaggacag aagcgggttc ttcatccgca
      901 aaaaaattct ttcaaccaaa ggaaggaact agagtcacat gaatacgctc gccatcgata
      961 aagccattgc caaggtgccg tttttggcgg attctaagaa catcaaaaag attccgctca
     1021 gtggtggcat taccaacttg aattttaaga tcgaagcgga tggtcgttct tacgtcattc
     1081 gccttgcagg tgaagggacc gaccagctcg gcatcaaacg cgatgtagaa tatgccgcta
     1141 ataaagtagc tggtgaattg ggcattgcgc ccgaggtggt gtacttcatt gaacccgaag
     1201 ggtatattgt gacccgcttt gtcactggca aacgcatcat gccagatgaa atcgtcaagc
     1261 cagattatct gacgcgcgtt gcgcaaaaac tgcgcttgtt ccaccgcaat gctcccaagt
     1321 tgaaaggcga gttcaacgtc ttccgccgcg tggaaatgct caccaaaacc gccaaagcca
     1381 agggcgcgca ctttcctgaa gattgggcgt tcattcaaaa gaaaatgcgc gaagcagaaa
     1441 aggcattgtt gaaagacccc tacaaaccca ctccctgcca caacgatttg ctcaacctca
     1501 actggttgga tgaagaagtt gcgggcgata ttggcgagat ccgcttgctc gattgggaat
     1561 atgcgggcat gggcgatatt ttctttgacc tcgggaattt ctcgcaccac catcgtttga
     1621 acgaggatca aatccgcatc ttccttaccg cttattttgg gaagttcacc cccaagcatt
     1681 atgcgcggct gagaattatg tgggcgatgt ctgagttgca cgaagccatg tggggcacaa
     1741 cccaatcggt catctccacg ttggaagaag atttccaagg ctatgccaat ttgtggttcg
     1801 ggcgctatcg catgcatgtc actgactatc gctgggaaca gtggttgaaa gatgcgtcga
     1861 agaagggcaa gtaagttagt tcataagtag ttggtaattg gcgaaagagg aaagaagata
     1921 gtttattttc atttttcctc tttcgataca aactcgaaac ctgaaaagaa aaaaccaagg
     1981 agtaccaaat gtctatcgta aatgcaaaag agattatgat ccctgcggca aaagaaggtt
     2041 gggctgtggg cgcgttcaac gtcaccgact tgctgcaatt tgaagcggtg atcgacgcgg
     2101 caattgaaaa gaaagcccct gtcattgtgc agacttctgt gaagccttca cagttcctcg
     2161 gcaccaatat gatggtagcg atctaccgca cattggctga atctgcgcct gtgccggtgt
     2221 gtttgcacct cgatcacagc accgatatca actactgcaa gaagtgcgcg gatgctgggt
     2281 ataccaacat catgatcgac gcttccaaac agtcctacga agagaacatc cgccagacca
     2341 aagaagtggt ggattatgct cacagcgttg gcaacatttc tgtggaaggc gaactcggaa
     2401 ccgttggtgg tgtggaagac cagatcaagg tcgcggaaga tgaagcgcaa ctcgccaacc
     2461 ccgaacaatc cattgaattc gtcgaacgca ccggcgtgga cattttcgct cccgccatcg
     2521 gcaccgcaca cggcgtgtac aagaccaaga atcccaagat cgatttcgaa cgcatggcga
     2581 ccattcacaa gatgctgaac agcaatggca tcaagacccc gctcgtcgtc cacggcggaa
     2641 ccggtttacc cgatgattat gttgccattg ctgttcagca tcttgtgaat ggt
//
LOCUS       DAOQNJ010000388         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28519_length_2689_cov_9.523538, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000388 DAOQNJ010000000
VERSION     DAOQNJ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28519_length_2689_cov_9.523538"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLG52_10490"
     CDS             <1..264
                     /locus_tag="PLG52_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41918.1"
                     /translation="DEYQSIEGCVINDPMTLALTYMPEICDYQNLVMDVDISTGVGLG
                     NTFADVYNYEKKKPNMKVAMGVRPRMFMELFLERMEKFSQEIS"
     gene            329..1018
                     /locus_tag="PLG52_10495"
     CDS             329..1018
                     /locus_tag="PLG52_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41919.1"
                     /translation="MLDKIKSNFNTQTWVLIPIAIAINLAIGQIVLVLKLPVFLDSIG
                     TVMVGVLCGPWAGALTGALSNTIAGIALDPGWFPWWPVAAMIGYMAGRMAGWGFFKSW
                     WRVAVTGFVIALTAAIVSTPIAVYLFGGITASGSSFITAYLLATGQGIWSAVVSTSFL
                     TEPVDKITTAMIAFAIIQGLPKRTIGGYPKADQVAAEGGESKNQLYIAVGVIVVLVLF
                     AAFMLRNLLGG"
     gene            1095..1874
                     /locus_tag="PLG52_10500"
     CDS             1095..1874
                     /locus_tag="PLG52_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNB41920.1"
                     /translation="MHERLSFHIQRDSLLHRLNPLTKMVIILTIILISFTFPYYWTPH
                     LLLLIVILPLSLTGKVFREFSLTALRLIIPAAGFLFLMQAFFQPIGETVIFKFYFLDM
                     TMESVMFAFRNAMRIVVMVSGFTIFLLTTHPSELMSDLTRRGLPGQFAYVIISTLQIL
                     PQMQAKAQTIISAQRSRGLDTESSFLKRAGSLIPLIGPLVFGSLVEVEERAIAIEARG
                     FTSKQTKTSLHEIPDTASEKTLRWGLLALIVISIGLNLWLS"
     gene            1862..>2689
                     /locus_tag="PLG52_10505"
     CDS             1862..>2689
                     /locus_tag="PLG52_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41921.1"
                     /translation="MAFVNIQNLTYKYPLVKEPVLRNITAQIQEGEFVSIVGPNGAGK
                     STFCYALAGFAPHFFKGEFSGEIEVSGKASSKSTLDEWVLNVGLAFQNPFNQISGAKY
                     TVFEEIAFGLENIGVPREEIKSRVQDAMSLTGISELADRSPYSLSGGQQQRVALTSIL
                     VMRPKLLVLDEPTSQMDPIGTREVFGVVRKMAEEGMTVVMVEHKVEWIAEFADRVIAL
                     KEGQILLEGKPNEVLTSDVLAESGFGISRYTSTAREAKKRGLWKKETLPVTLDEAVEG
                     "
BASE COUNT          645 a          694 c          641 g          709 t
ORIGIN      
        1 gatgaatatc aaagcatcga aggctgtgtg atcaatgacc cgatgaccct ggcgctgacc
       61 tacatgccag agatctgcga ttatcaaaac ctcgtgatgg atgtggacat ttccactggg
      121 gtcgggctcg gcaatacctt tgcggatgtc tacaactacg agaaaaagaa gcctaacatg
      181 aaagtggcga tgggcgtacg tccgcgcatg ttcatggagt tattccttga acgaatggaa
      241 aaattttcac aggaaatatc gtgacaatct gaaaaataca gtgttatcat tgctgtagct
      301 ttataactta attcactcgg aggatagcat gctggataaa atcaagagca atttcaacac
      361 gcaaacgtgg gttctcatcc ccattgccat cgcaattaat cttgccattg gtcaaattgt
      421 gctagttctt aagttgccgg tcttcctgga tagcattggc accgtaatgg ttggtgtgct
      481 ttgtggtcct tgggctggtg cgcttactgg tgccttatcc aataccattg cagggattgc
      541 gctcgaccca ggttggttcc cttggtggcc cgttgccgca atgatcggct acatggcggg
      601 acgcatggct ggctggggct tcttcaaaag ctggtggcgc gtggctgtga ccggtttcgt
      661 gatcgccctc accgctgcaa tcgtctccac cccaatcgct gtgtacctct ttggcggtat
      721 cactgccagc ggctcttcct tcattaccgc atatctgctc gctaccgggc aaggcatttg
      781 gtctgctgtg gtcagcacca gcttcttaac cgagccggtc gataaaatca ccactgccat
      841 gatcgccttt gccatcattc agggcttgcc caagcgcacc attggcggct atcccaaggc
      901 agatcaggtc gcagccgaag gcggcgaaag caagaatcag ttgtacatcg ccgtgggtgt
      961 gatcgtcgtt ttggtgttgt tcgcagcctt tatgctcaga aaccttctgg gcggctaaac
     1021 taaagctcgc ttttcaaaaa gagaccagac attctggtct cttttttgtt gccaagtggg
     1081 tataatccca ccctatgcat gagcgccttt ccttccacat ccaacgagac agtcttctgc
     1141 atcgtctcaa cccgttgacc aagatggtga tcatcctaac catcattttg atctccttta
     1201 ccttccccta ttactggacg ccccatctgc ttttattgat cgtcattctt ccgctcagcc
     1261 taacgggaaa agtgtttcgc gaattttccc tcacggcact gcggctcatc atccctgcgg
     1321 ctggttttct ttttctcatg caagccttct tccagccgat tggcgaaacc gtgatcttca
     1381 aattctattt tctcgatatg accatggaaa gtgtcatgtt cgccttccgc aatgccatgc
     1441 gcattgtggt catggtttca ggtttcacca tctttttgtt aactacacac cccagtgagt
     1501 taatgtccga ccttactcgg cgtggattgc ccggtcagtt tgcttacgtc atcatctcta
     1561 cactgcaaat tttgccgcaa atgcaagcca aagcacaaac catcatctcc gctcagcgct
     1621 cacgcggact cgacacagaa agcagctttc tcaaacgcgc tggttcgctc attccgttga
     1681 tcggtccgct tgtgttcggc tcactcgtcg aagtggaaga acgtgccatc gccattgagg
     1741 cgcgtggctt tacatccaaa caaaccaaaa cctcactgca tgaaattcca gatacagcct
     1801 ctgaaaaaac tttacggtgg ggcttgctcg cattaatcgt catttccatt ggattgaatc
     1861 tatggctttc gtaaacattc aaaacctcac atacaaatat ccacttgtca aagagccggt
     1921 gctacgaaac attacggcac agattcaaga aggtgaattc gtttccattg taggtccaaa
     1981 tggcgcaggc aaatctacgt tttgttatgc attggctggg tttgcgccgc acttttttaa
     2041 aggcgaattt tcaggcgaaa tcgaagtaag tggaaaagcc tcctccaagt ccacattaga
     2101 cgaatgggtc ttaaatgttg gcttggcgtt tcaaaatccc ttcaatcaaa tttccggcgc
     2161 aaaatatacg gtctttgaag agattgcttt cgggttggaa aacatcggtg tgccgcgtga
     2221 agaaatcaaa agccgcgtac aggatgccat gtccctgacc ggaatcagcg agttagccga
     2281 tcggtctccc tactctctct ctggcggaca gcaacaacgc gtcgcgctga cctcgatcct
     2341 ggtcatgcga cccaagcttt tggtgctcga cgaacccacc tcgcaaatgg accccattgg
     2401 cacccgcgaa gtctttggcg tggtgcgcaa aatggctgaa gaaggcatga ccgttgtgat
     2461 ggtcgagcac aaagtggaat ggatcgccga gtttgcagac cgtgtgatcg ctttgaaaga
     2521 agggcaaatc cttttagaag gtaaacccaa cgaagtgtta acctcagatg tcttggcaga
     2581 gagcggattc ggcatttccc ggtatacatc cacagcacga gaagcaaaga aacgcggact
     2641 atggaaaaag gaaacattac ccgtcacgtt agatgaggcg gtggaaggg
//
LOCUS       DAOQNJ010000389         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28609_length_2684_cov_6.573602, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000389 DAOQNJ010000000
VERSION     DAOQNJ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28609_length_2684_cov_6.573602"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLG52_10510"
     CDS             <1..605
                     /locus_tag="PLG52_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB41922.1"
                     /translation="TYGKQRGYEGSKYSEHQIFHQWYPDLTNLDQATAFCNNANSAIR
                     KSLWDLHPYDETLTGLEDLEWSKWAKGQGYKIAYVAEAEIIHIHNETPRGVYNRYRRE
                     AMALRRIYPETNFNLYDFFRLTITNIISDLWHAARERVLLKSFASIFWFRFAQFRGTY
                     MGHRETSLVTPQLRETFYYARERKKKEERKRDVEPIQYKK"
     gene            639..965
                     /locus_tag="PLG52_10515"
     CDS             639..965
                     /locus_tag="PLG52_10515"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNB41923.1"
                     /translation="MEKISKRAIQTFARKVAKEFKPQKIILFGSYAYGKPTADSDVDV
                     LIVMSFSGRNPEKATEIWMATKPKFPVDIMVRKPSELKKRLAMGDFFLQDVVTMGKVL
                     YEATHA"
     gene            946..1332
                     /locus_tag="PLG52_10520"
     CDS             946..1332
                     /locus_tag="PLG52_10520"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNB41924.1"
                     /translation="MKPLTREWVEKAENDWRSLHREIRARKDPNYDAACFFAQQCVEK
                     YIKARLVEADIYFKKVHDLTYLLGVVIEVEPLWISYEQDFRLLTDYAVEFRYPGASAD
                     LINAKNALKTCKSFRTTARFSLRIEE"
     gene            1339..2172
                     /locus_tag="PLG52_10525"
     CDS             1339..2172
                     /locus_tag="PLG52_10525"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB41925.1"
                     /translation="MPSLSLSLTPAHEHPIPFLADLMTRSFEGYLVPINITDTVLLAM
                     LRRDGIDLTASRVLMRDGEPIGLAMIARRGWTSRLAAMGITSNARSGGVGTWAMEQLI
                     EEAKARGEKEMLLEVIEQNTAGVKLYEKVGFTKIRRLVGYKLENPVSDSDEQLEEIDI
                     REVARQVTYHGMKDLPWQLSGTSIMQHTPPSRAFRINDAYCLISNPDATDVAISSVLV
                     KARSRGAGLSAVLMRALFAKFPNKIWHVSPIYPEEMGFIFEHIGMVRETLSQWQMSRA
                     L"
     gene            2192..>2684
                     /locus_tag="PLG52_10530"
     CDS             2192..>2684
                     /locus_tag="PLG52_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNB41926.1"
                     /translation="MKIAALVPMRHHSQRVPGKNYRPLAGKPLFQHILETLQSVPEID
                     TVIVDTDSEPVMDGVRRLFPNVELVARPEHLRADDVPMNDILLYDTAQVQADFYLQTH
                     STNPLLKADTISRGIKSFLNDYPKYDSLFSVTRLQTRLYWQDGRAINHNPLELLQTQD
                     LPPV"
BASE COUNT          704 a          699 c          650 g          631 t
ORIGIN      
        1 tcacctacgg caagcaacgc ggatacgaag gctcgaaata ttcggagcat cagatattcc
       61 atcagtggta tcccgatctg acgaacctcg accaagcgac tgccttttgc aacaacgcca
      121 attctgccat ccgcaaaagc ttatgggact tgcatcccta cgatgaaacg ttgactggtt
      181 tggaagacct cgaatggagc aaatgggcga agggacaagg ctataagatc gcctatgtcg
      241 ccgaagcgga aatcattcac attcacaacg agacgccgcg cggagtgtat aaccgctacc
      301 gccgtgaagc gatggcattg cgccgcatct accccgaaac gaatttcaac ttgtacgatt
      361 tcttccgcct gaccattacc aacatcatca gcgacctgtg gcatgccgca cgtgaaagag
      421 ttttgttaaa aagtttcgct tccatctttt ggtttcgctt tgcacaattt cgaggaacat
      481 atatggggca tcgtgagacc agccttgtca cgccgcagtt gcgggagacg ttttattatg
      541 cgcgtgaacg aaagaagaaa gaagaaagaa agcgggatgt ggaaccgatt cagtataaaa
      601 agtgaatcgt tgcttctgga ttcggtataa ttacctctat ggaaaagatt tccaaacgag
      661 ccattcaaac tttcgctcgc aaagttgcca aagaatttaa gccccaaaag attatccttt
      721 ttggttcgta tgcgtatggc aaacctacag ccgattcgga tgtggatgtt ttgatcgtaa
      781 tgtcattctc cgggcgaaac cccgaaaaag cgaccgaaat ttggatggcg accaagccga
      841 agtttcctgt ggatattatg gttcgcaaac cgtctgagtt gaagaagcgg cttgccatgg
      901 gcgatttttt cttgcaggat gttgtaacca tgggaaaagt cctttatgaa gccactcacg
      961 cgtgaatggg tggaaaaggc ggaaaatgat tggcgctcat tgcaccgtga aattcgcgcg
     1021 cgaaaagacc caaattatga tgctgcctgt ttcttcgccc aacaatgtgt agaaaaatat
     1081 ataaaagcaa gattggttga agcagatatt tatttcaaaa aagttcacga tctgacatac
     1141 ctccttgggg tggttatcga agttgaacct ctctggatca gctatgaaca agattttcgc
     1201 ctgttgacag actatgccgt tgaatttcga tacccaggcg cttcagctga tttgataaat
     1261 gcaaaaaacg cgctgaagac gtgtaagagt tttcgcacta cagctcgatt cagtttgaga
     1321 atcgaagaat aaattttcat gccttccctc tccctctccc tcacccccgc ccacgaacac
     1381 cccatcccct tcctcgcgga cttgatgacg cgcagttttg aaggctatct tgtcccgatc
     1441 aacatcacag acacggtctt gcttgccatg ttgcgccgcg atggcattga cctgaccgcc
     1501 agccgcgttt tgatgcgaga tggtgaaccc attggtttgg caatgatcgc ccgccgtggt
     1561 tggacgagtc ggctcgcggc aatgggcatc acatccaatg ctcgcagcgg cggtgtaggc
     1621 acatgggcaa tggagcagct gatcgaagaa gccaaagcac gcggcgaaaa agaaatgctg
     1681 ctcgaggtca ttgagcaaaa tacagctggc gtgaagctgt acgaaaaagt tggcttcacc
     1741 aaaattcgca ggctggtggg ctacaaactc gaaaaccctg tctcagattc cgatgagcag
     1801 cttgaagaaa tcgacatccg cgaagtggcg cggcaggtga cctatcacgg catgaaagat
     1861 ctaccgtggc aactctctgg cacgtccatt atgcagcaca cgccgccctc acgcgcgttc
     1921 cgcatcaacg atgcctactg cctcatcagc aatcctgatg cgacagacgt ggcaatttcg
     1981 tcagtgttgg tcaaggctcg gtcacgtgga gcaggcttga gcgcggtgtt gatgcgcgcg
     2041 ctcttcgcaa aattccccaa taaaatttgg cacgtctcac cgatctaccc cgaagagatg
     2101 ggcttcatct tcgaacatat cggcatggtg cgcgagaccc tttcacagtg gcaaatgtcc
     2161 cgcgccttat aagacaaagt aaaataaagg catgaaaatt gccgcccttg ttcccatgcg
     2221 ccaccatagc cagcgcgtgc ctggtaaaaa ttatcgcccc ctcgcgggca aacccctctt
     2281 tcaacatatt ttggaaacgc tgcaatcggt gccagaaatt gacaccgtga tcgtggatac
     2341 ggactccgag ccggtgatgg atggagtgcg ccgcctcttc cctaacgtgg agctggttgc
     2401 ccgccccgag catctccgtg ctgacgatgt tcccatgaac gacattctgc tctacgatac
     2461 ggcgcaggtg caggcagatt tttacctgca aacccatagc acgaatccat tgctcaaagc
     2521 cgataccatc tcacgcggaa tcaaatcgtt tctcaacgat taccccaagt atgactcgct
     2581 attttcagta acacgtttac aaacccgcct ttactggcag gacggacgtg ccatcaacca
     2641 taatccgctt gagttgttgc aaactcaaga cctgccgccc gtgt
//
LOCUS       DAOQNJ010000390         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28673_length_2681_cov_8.106245, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000390 DAOQNJ010000000
VERSION     DAOQNJ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28673_length_2681_cov_8.106245"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLG52_10535"
     CDS             <1..433
                     /locus_tag="PLG52_10535"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNB41927.1"
                     /translation="VMKILKKNLADEVTWQYEGEELSRDKNSITIEALFNRDDMPFQE
                     IVLKRNDRFVETFYSDKWYNIFAIYDRDDGSLKGWYCNITKPAIIEIDSVSYVDLALD
                     LWVSANGKQTVLDEDELEELKLGEALKENVYAGLRELQAFF"
     gene            complement(478..630)
                     /locus_tag="PLG52_10540"
     CDS             complement(478..630)
                     /locus_tag="PLG52_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41928.1"
                     /translation="MSEEMPVAPSETPAPNNNNRNIIIGVVAIVVLCCCCVLGSGIGY
                     YVWNNF"
     gene            complement(677..1249)
                     /locus_tag="PLG52_10545"
     CDS             complement(677..1249)
                     /locus_tag="PLG52_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNB41929.1"
                     /translation="MELLLGIFTAFGLSASAGLNAYIPLLVVGVIAHYAPNTLNLASP
                     FDLLANPWILILLGVLVIIEMVADKVPAVNHINDLIQTIIRPVAGAIAFAASANVITD
                     VNPVLALACGLLVAGSVHTVKAAAVRPAVTATTAGAGNIPVSIAEDVIATITSILAIL
                     IPLIAGTLFIIFLVLVLWWWASRKQKAQSA"
     gene            complement(1309..2157)
                     /locus_tag="PLG52_10550"
     CDS             complement(1309..2157)
                     /locus_tag="PLG52_10550"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNB41930.1"
                     /translation="MQTNPQPSKLETIASWVVPIAAIAAVIAWFIIAPEGALGKLDAI
                     GYAVCHRIDVRSFHIGDRQLPLCARCTGEFYAAGLTLLFQMVVSGKRSKLPSRGVMAV
                     LFGFFVAFGVDGTNSYLYLLKETGRNSGFLAQIPNLYVPNNVLRLFTGSGMGIALAAM
                     LYPVINQTLWRTLDDRPAIEWKQLGILVAGIIGINLLVLTDSPFVLYPIAYISALGTL
                     SLLVIVFGLLWLIIMKQDNSFEHPRQLWLAFASGLTLALLLILSIDLMRLNFTGTWSG
                     FPGLSG"
     gene            2248..>2681
                     /locus_tag="PLG52_10555"
     CDS             2248..>2681
                     /locus_tag="PLG52_10555"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNB41931.1"
                     /translation="MKRIGILTGGGDAPGLNAVIRAAVKTAINEYQCEVIGIRDGYDG
                     FLDTQNGIVPLGMEEVRGILPRGGTILGAANRGNPYARKVIRDGKEVTIDVSDEIVKG
                     IKELGLDALLVLGGDGTLHIAHELAQKGVPVIGVPKTIDNDIG"
BASE COUNT          724 a          554 c          764 g          639 t
ORIGIN      
        1 tgtcatgaaa atcctgaaaa agaatctggc agacgaagtg acttggcagt atgagggcga
       61 ggagttgagt cgtgataaga attcgatcac gattgaagcg ttgtttaatc gtgatgatat
      121 gccgtttcaa gagatcgtac taaaacgcaa cgaccgcttt gtggaaactt tttattctga
      181 taaatggtac aacatttttg ccatttacga ccgtgatgat ggcagcctga aaggctggta
      241 ctgtaatatt accaagcccg ctattattga aattgactcg gtttcatacg ttgaccttgc
      301 gcttgatctt tgggtctctg caaatggaaa acagaccgtt ttggatgaag atgaattgga
      361 agaattgaag ttgggcgagg cattgaaaga aaatgtatat gccgggttgc gtgaattgca
      421 agcgtttttt taatcaaaaa aatcccctgc catgagcagg ggattttttg ttggaaatta
      481 gaaattattc cagacgtagt aaccgatacc agagccaagc acacagcagc agcacaacac
      541 gacaatggca acgacaccga tgatgatatt gcgattgtta ttgttaggcg ctggggtttc
      601 ggatggggcg acgggcattt cttcagacat gatttctctc cttttagaaa gttatttttt
      661 tggattataa acgatgtcag gcagactgtg ccttttgttt gcggcttgcc caccaccaca
      721 ggacaagcac taggaagata atgaacaaag ttccagcaat tagcggaatg aggatcgcta
      781 gaatagaagt gatggtggca atcacatctt cagcgatcga aactgggata ttgcccgccc
      841 ccgctgttgt ggctgtgacc gctgggcgca cggctgctgc ctttacggtg tgaacactgc
      901 cagctaccag caaaccgcag gcaagcgcca gcacggggtt gacatcggtg atgacattgg
      961 cactggcggc gaaggcgatg gcacctgcga ccgggcggat gatggtttgg atcaggtcgt
     1021 tgatgtgatt gaccgcgggt actttatctg ccaccatttc aatgatgacc aacacgccga
     1081 gtaggatgag gatccacggg ttggcgagca ggtcgaaagg ggaggcgagg ttgagggtgt
     1141 tgggagcgta atgagcgatg acacccacca ccaaaagtgg aatgtaggca ttcaacccgg
     1201 cgctggctga aagaccaaag gcagtaaaaa ttccaagtaa aagttccatt tcttctcctt
     1261 atcaaagatc gtacaacttg attatacgat ttttgagtat attcgttgtt agcctgatag
     1321 accggggaac ccgctccatg tgcctgtgaa gttgaggcgc attaggtcaa tgctcaagat
     1381 gagcaacaat gccagggtca agccggaggc gaacgccagc caaagttgac gtggatgttc
     1441 gaaggagttg tcttgcttca tgatgatgag ccatagcaac ccgaagacga tcaccaacag
     1501 cgaaagagtg cccaaggcgc tgatataagc aatggggtac aacacgaagg gactatcggt
     1561 aagaacaagt aagttgatgc cgatgatgcc cgctaccaga atcccgagtt gtttccattc
     1621 gatggcgggg cggtcatcca aggtgcgcca gagggtttga ttgataaccg ggtagagcat
     1681 ggcagcgagg gcgatgccca tgccgctgcc cgtaaagagg cgcaagacat tattgggcac
     1741 atatagattt ggaatttgcg ccagaaaacc agagtttctg ccggtttctt ttaacaagta
     1801 caggtaagaa ttggtgccat ccacgccaaa ggcgacgaag aaaccaaaga gcaccgccat
     1861 gacgccgcgc gaagggagct tgctgcgttt tccacttacg accatttgga aaagcagggt
     1921 aagcccggcg gcatagaatt caccggtaca gcgggcacat agggggagtt gacggtcgcc
     1981 aatgtggaag gaacgtacgt caatgcgatg acagacagca tagccaatgg catcgagctt
     2041 acccaaggcg ccttcaggcg cgataataaa ccaggcgatt acggctgcaa ttgcggcaat
     2101 gggaacgacc caactggcaa tagtttcaag ttttgagggt tgtggatttg tttgcatttt
     2161 gagattatac ccagcctgat gtgaaaatct ctcttttgag gaatatttaa tattttgcgt
     2221 tatcatcttc catatagagg tgacatcatg aaacgaattg gaatccttac aggcggcggc
     2281 gatgcgccgg ggttgaatgc ggtcatccgt gcggcggtaa agacggcgat caatgaatat
     2341 cagtgcgagg taatcggcat ccgtgatggg tatgacggtt ttctcgatac acagaatgga
     2401 attgtgcctt tgggcatgga agaggtgcgc ggaatccttc cgcgtggcgg gacgattctt
     2461 ggggcggcga atcgtggtaa cccgtatgct cgcaaggtga ttcgagatgg caaagaagtc
     2521 acgattgatg tgtcggatga aatcgtgaaa ggcattaaag aattgggctt ggacgcattg
     2581 cttgtgcttg gtggagacgg cactttgcat attgcacatg aactggctca aaaaggagtg
     2641 ccggtgatcg gtgtgcccaa gacgattgat aacgacattg g
//
LOCUS       DAOQNJ010000391         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28878_length_2670_cov_8.746080, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000391 DAOQNJ010000000
VERSION     DAOQNJ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28878_length_2670_cov_8.746080"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2293
                     /locus_tag="PLG52_10560"
     CDS             <1..2293
                     /locus_tag="PLG52_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41932.1"
                     /translation="AESVPSGTEPKAPKQPRVSDDRDYDIDGSNKDKSLHPEVKPHVF
                     VVMPFGNKKGADGHPFDFNAIYKILIKPAIEDAGFEPFRADEETASGDILTDMFQELL
                     LADMVICDLSIDNANAFYELGIRHALRKRGVVHIQAGRAYMPFDVFNVRTLPYHITSE
                     GLPDPAYIRSDIKAIARLLKDTWASDRELVHSPIYNLLTGLKEPSRKSLQTPLATGFW
                     REYEEWKDRVTIAQRNKHIGDILLLTEEIRNPLIREDAVAEVGGALARLGRNELALKQ
                     YREGIDVNGENLEFRREEAFHLNRVGRVNEAIVKLESILNDYPEDNKSISYLGRIYKE
                     MWTNSWIKVKDKNKRLRLAFESYHWLIQSIKIYMKGFQVDLRDYYPGINAYTLSKIAI
                     HLADKFDNKKTPDPDITRIRHRLHGLQETLEFCLENRLDLDQEKADYWSLVSLAELRL
                     FTSNDIAEVLRSYRKAVTAVRRDLFSLRSSLEQLETIHSLGIRTEFVSACIKMFKEEI
                     KNASTGDAGNVIKVDNLVRPGTKNKGARALVFSGYMVDFAGKDKKTFPPEKESEIRQE
                     IKKRVDKFKPGPNDRAFLAGLSAGSEIIFAEICAEAGIKVKVFLPHSDSSYIRRFVTP
                     AGESWVDRFYKIRNHSLVDEIYQIEHLGQPKDDDNVYERNSRWAMYSAMGRVGIDNMT
                     LIAVASEFIGDTKDRDIQITRYTIELMRNLGGRVEEFINPTKYIYSVIDSALERLIAQ
                     GGSSGEKKITPTKLRKRKSERKV"
     gene            2342..>2670
                     /locus_tag="PLG52_10565"
     CDS             2342..>2670
                     /locus_tag="PLG52_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNB41933.1"
                     /translation="MNKLFVIVGASGVGKTTLMNALLTKYDCAIAYEQHDERPFQKLF
                     KEDARYALANQVDYLLMRAEQERELRSGNKPALMDGGFDLDFYGFTRLFHTHGWLSAA
                     EFDLCRRL"
BASE COUNT          792 a          614 c          600 g          664 t
ORIGIN      
        1 ggcagaatcg gttccatcgg gaacagagcc caaagcgcca aaacagccac gagtttcgga
       61 tgatcgggac tatgacatag atggctccaa caaagataag tctctacatc ctgaggtcaa
      121 accgcatgtt tttgtcgtca tgccatttgg taataaaaag ggagccgatg gtcatccgtt
      181 cgatttcaat gccatttata aaatcctgat caaacctgcc attgaagatg cgggctttga
      241 accatttcgt gcggatgaag aaaccgcttc aggcgacatc cttacggata tgttccaaga
      301 gttgcttttg gcagatatgg tcatttgcga tctaagcatc gacaacgcca acgcttttta
      361 tgaattagga attcgtcatg cgttacgcaa acgtggtgtg gttcatattc aagccgggcg
      421 cgcttacatg ccatttgatg tgttcaacgt acgtactttg ccctatcaca tcacatcaga
      481 aggtctgcca gatccggcat atattcgcag tgacattaag gcaattgcgc gccttctcaa
      541 agatacctgg gcttcagacc gagaactagt acatagccca atctacaacc tgctcacagg
      601 gcttaaggaa ccaagccgca aaagtttgca aaccccctta gcaactggct tttggcgcga
      661 atatgaggaa tggaaagaca gagtcaccat tgcgcaacgc aataaacaca ttggtgatat
      721 tttgctctta accgaagaaa tcagaaaccc gctcattcgc gaagatgcag ttgctgaagt
      781 aggcggcgca cttgcccggt tgggacgtaa cgagttagct ctaaaacaat accgcgaagg
      841 aatcgacgtc aacggggaaa atcttgaatt tcgccgggaa gaagcttttc accttaaccg
      901 tgtaggaagg gtaaatgaag ccatcgtaaa acttgaaagt attttgaatg attatccaga
      961 agacaacaaa tcaatttcat atctcggacg catctacaaa gagatgtgga ccaactcttg
     1021 gatcaaagtt aaagacaaga acaagcgcct tcgcttggca tttgaaagct atcactggct
     1081 catacaatcc atcaagattt atatgaaggg atttcaagtt gacctgcggg attactaccc
     1141 tgggatcaat gcatacaccc tttccaaaat tgcaattcac ctcgccgata agtttgacaa
     1201 taaaaagaca cccgacccag atatcactcg cattcgccac agactgcatg ggttgcagga
     1261 aacgctagaa ttttgtctgg aaaatcggct cgatcttgat caagaaaaag ctgattactg
     1321 gtctcttgta tctttggcag aattaaggct cttcacttcc aatgacattg ctgaagtttt
     1381 gcgctcctat cgcaaggcag tcacagccgt tcgacgcgat ctattttctc tgcgttcatc
     1441 ccttgaacaa ctggaaacga ttcattctct cggcattcgc actgaatttg tttcagcttg
     1501 tatcaaaatg ttcaaggaag aaatcaaaaa tgccagcaca ggcgatgcag gcaatgttat
     1561 caaggtagat aacttggtac gcccaggcac gaaaaacaaa ggggcccgag cactcgtatt
     1621 tagtggctac atggtcgact ttgcgggaaa agataaaaag acattcccgc ccgaaaaaga
     1681 aagtgaaatc cggcaggaaa tcaagaaacg tgttgacaag ttcaaacccg gacccaatga
     1741 ccgcgcattt ttagcggggt tatctgcggg tagtgaaatc atcttcgcag agatttgtgc
     1801 cgaagccggc ataaaggtca aggtgttctt accccactct gattccagtt atattcgccg
     1861 tttcgtaaca cccgctggcg aaagttgggt ggatcgcttc tacaaaattc gcaaccactc
     1921 attagtagat gagatctacc aaatcgagca cctcggtcag cccaaagatg acgataatgt
     1981 ctatgagcga aacagccgtt gggcaatgta ttcagccatg gggcgcgttg gcattgataa
     2041 catgacacta atagctgtag caagcgaatt cattggcgac accaaagacc gcgatattca
     2101 aatcactcgt tacacgattg aattgatgcg caatctgggt ggcagggttg aagaatttat
     2161 caaccccacc aagtatattt acagcgtaat tgatagcgcc cttgaacgcc tcattgcaca
     2221 aggtggttct tcaggcgaaa agaaaattac acctaccaaa ctgaggaaaa gaaaatctga
     2281 aagaaaggtt tagcaaaacc atagacccgc ttttgcagcg ggtctattca tcctaaacgc
     2341 tatgaataaa ctttttgtca ttgtaggtgc cagcggagta ggcaaaacca cgcttatgaa
     2401 tgccttgctt acaaagtatg attgcgctat tgcatatgag caacatgacg agcgcccatt
     2461 ccaaaaactt ttcaaagaag atgcacgcta tgcgctggcg aatcaagttg attatctatt
     2521 gatgcgcgcc gaacaagaac gtgaattacg ctctggcaat aaacccgctt tgatggatgg
     2581 cggctttgac cttgattttt atggatttac acgcctcttt catactcatg gctggctcag
     2641 cgctgcagaa tttgacctgt gtcgacggct
//
LOCUS       DAOQNJ010000392         2667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_28933_length_2667_cov_6.917688, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000392 DAOQNJ010000000
VERSION     DAOQNJ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28933_length_2667_cov_6.917688"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLG52_10570"
     CDS             <1..554
                     /locus_tag="PLG52_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB41934.1"
                     /translation="QKPDLIIVDWMMPKMDGISFIQRLRDEENQTMILMLTAKDAIDD
                     RVDGLESGADDYLIKPFAPAELVARVHAMLRRVETKPENQKVSYADVSLDPTSREAKR
                     GTIELNLTVTEFNLLHMLLRHPRQVLERRQILNEVWGYDFGGDDNVLEIYIGYLRKKL
                     EVDGNSRLIQTVRGVGYVLREES"
     gene            554..2062
                     /locus_tag="PLG52_10575"
     CDS             554..2062
                     /locus_tag="PLG52_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB41935.1"
                     /translation="MSIRLRITFLYTFILALTLTVFGLALYTIQAQSTMDSLKHDLSV
                     SANRLADATLKTDFPNFNGNPPPNGQEPPNGQPPNGQEPPPGGQQPGDGNPPPTPIPF
                     DQFSNQQEFQSFSEREIARVLDVQGNLVSSPFGREGDALPLSAAGLQALQNGSDWWET
                     NIVSDESMLTYNRPVIVDNKVIYIVQVARSLTERNRTLQSLSTTLLLAGLLTTLIAFG
                     IGWLFSGLTLAPINRITKTAKEIGEERDFSRRVDYNGPQDEVGQLASTFNAMLTRLQD
                     AFKKVEHSLQMQRNFVADVSHELRTPLTTLRGNLGLLKRNPSPEIQNDILSDMVDESD
                     RLIRLVNDLLLLARADAGRSLSKEPVDVSIVLQEVCRQGNQLDANREINLDVPTDTSI
                     IGDRDAFKQVALILLDNALKHSTGKINIRAEKYGKHIDVRVQDFGEGIPPETLAHVFD
                     RFYRGEDAATIPGFGLGLPIAKTLVEGFGGEIKIESEVGKGSTVIMRFPSTK"
     gene            complement(2066..>2667)
                     /locus_tag="PLG52_10580"
     CDS             complement(2066..>2667)
                     /locus_tag="PLG52_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNB41936.1"
                     /translation="RDNIIYHKDELLQEADVPDIFKVLDQIANRVPAGANGVMYTPWI
                     WGERAPVEDKTLRAGLYNLSLNSTREDIIRAFLEGIAYNTRWLLSPVEKFLGRKVNSI
                     NIVGGGAQSDLWCQIFADVMNVEIKQVADPIYANARGAAWIAAAGLGEIKFADVPELI
                     QFRRAYQPQIKNRAVYDRGFENFQLIYKQIKGVYRRLNQ"
BASE COUNT          721 a          698 c          601 g          647 t
ORIGIN      
        1 cgcaaaaacc agacctgatc attgtagatt ggatgatgcc caaaatggac ggcatctctt
       61 ttatccaacg gttacgagat gaagaaaatc aaaccatgat tctgatgctc acagcgaaag
      121 atgccattga cgaccgcgta gacgggttgg aaagcggcgc tgatgactat ctaattaaac
      181 cttttgcccc agctgaactg gttgcgaggg ttcatgccat gctgcgcaga gtagaaacaa
      241 aaccagaaaa tcagaaagtc tcttatgcag atgtcagcct cgaccccaca tcgcgcgagg
      301 cgaaacgtgg taccatcgaa ctcaatctca cagtgacgga attcaatctt ttgcatatgc
      361 tattgcggca tccacgccag gtgcttgaac ggcgtcagat tctcaatgag gtctggggct
      421 acgactttgg cggagatgat aacgttcttg agatctatat cggatatctg cgtaaaaaac
      481 ttgaagtaga cgggaattcg cgcctaatac aaaccgtccg cggggttggc tacgttctgc
      541 gtgaagaatc ataatgtcca ttcggcttcg catcactttt ctttatacat tcatcctcgc
      601 gttgacgtta accgtctttg gtctggcgct atacacaatt caagcacaat cgacaatgga
      661 ttcgctcaaa catgacctaa gcgtgagcgc caaccgttta gcagatgcaa cactgaaaac
      721 ggatttcccc aatttcaatg gcaaccctcc tccgaacggg caagaacctc caaatggaca
      781 accacccaac ggacaagaac ctccaccagg ggggcaacaa cccggagatg gaaacccacc
      841 tcctaccccc ataccgttcg atcaattttc caatcaacaa gaatttcaat cgttctcaga
      901 acgtgaaatc gccagagtcc tagatgttca aggtaatctg gtcagcagcc cctttggacg
      961 cgaaggggac gcattgcctt taagcgccgc tgggttgcaa gccttacaaa acggcagcga
     1021 ctggtgggaa acaaacattg tttcagatga aagcatgctt acctacaacc gccccgtcat
     1081 tgtagataat aaagttattt atatcgttca ggtagctcgt tcactcaccg aacgaaatcg
     1141 cacactgcaa tctttatcta caacattatt gcttgcaggt ttacttacaa cactgattgc
     1201 ttttggaatt ggctggcttt tttcaggtct tacattagcc cccatcaacc gcatcaccaa
     1261 aactgccaaa gagatcggtg aagagcgaga tttttctcgc cgggtcgatt acaacggtcc
     1321 acaagatgaa gttggtcaac tcgcgagtac cttcaatgcc atgcttaccc gcttgcaaga
     1381 tgcatttaaa aaagtagagc actctctgca aatgcagcga aactttgttg ccgacgtctc
     1441 acacgaattg cgcacaccgc ttaccacatt gcgcggaaat cttgggctgc tcaaacgcaa
     1501 tccttcgcca gagatccaaa atgatattct tagcgatatg gtcgatgaaa gcgatcgtct
     1561 tattcgcctc gtcaatgacc tgcttttact tgcacgcgcc gatgccgggc gtagtctctc
     1621 gaaagaacca gtggatgttt ctattgttct tcaagaagta tgcaggcagg gaaatcagct
     1681 tgacgcaaat cgagagatta acctagacgt tcccactgac acttcgatca tcggagatcg
     1741 tgatgccttc aaacaggtcg cactgatcct gctagataat gcacttaagc actccacagg
     1801 caagatcaac attcgcgctg aaaaatatgg aaagcatatt gacgttcgcg ttcaagactt
     1861 cggcgaaggg attccacccg agacccttgc gcatgtcttc gaccgtttct atcgcgggga
     1921 agatgccgca accattccgg ggtttgggct gggtctgcca atcgcaaaaa ctcttgttga
     1981 aggctttggc ggcgagatca aaatagaaag cgaagtcggc aaaggcagca cagtcatcat
     2041 gcgctttcca tctaccaagt agactttact gattcaaacg acgatacacg ccctttatct
     2101 gtttatagat caactggaag ttttcaaacc cacgatcata cacggctcta ttcttgattt
     2161 ggggttgata cgcccttcgg aattggatca actctggaac atcggcaaac ttaatttcgc
     2221 ccaagcctgc cgccgcaatc cacgccgcgc cgcgcgcatt ggcatagatg gggtctgcaa
     2281 cctgcttgat ctcaacgttc atcacatccg caaaaatctg acaccacagg tcggactgcg
     2341 ctcccccgcc aacgatgtta atgctattca ctttgcgacc caggaatttt tccacagggg
     2401 atagcaacca gcgtgtgtta taagcaatgc cttccaaaaa ggcgcggatg atatcctcac
     2461 gtgtggagtt gagtgacaag ttataaagtc cggcacgaag cgttttgtct tccactggcg
     2521 cacgctctcc ccaaatccat ggcgtgtaca tcactccgtt cgcaccggca gggacacggt
     2581 ttgcgatctg gtcgagcacc ttgaaaatat cgggcacatc ggcttcttgc agcaattcat
     2641 ccttgtgata gatgatgttg tcgcgca
//
LOCUS       DAOQNJ010000393         2647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_29320_length_2647_cov_11.305941, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000393 DAOQNJ010000000
VERSION     DAOQNJ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29320_length_2647_cov_11.305941"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PLG52_10585"
     CDS             <1..1028
                     /locus_tag="PLG52_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41937.1"
                     /translation="FQREWGKRIMRLSQAKIPTLLLIGNHDISPSIGRAHALQEFKTL
                     QVPYVKVLDAPELLKPEDLWDLPLQVIAMPWVARSGFMAATGETDSTQAFSRIEENIS
                     ELITGWIEECDLSMPIVLTAHASIEGAKFGGERLVMLGNDLVLTGGLVKNPKLNYVAM
                     GHIHKPQDVNEGHQPPVIYPGSIERVDFGEAKEERFFVIADVEKGRNTEVEWIQLTGV
                     RKFIDRRATIESIESVTEFLKSKLPKPKEMSEAIIRLIVEYPREWDSLIDESALRKYA
                     ADAFEFHFVKRPQIESRVRIDEGQVVSSLSPLDLLDQYFDAAKVSNADELKNLAQEII
                     SEDSQDS"
     gene            1081..2364
                     /locus_tag="PLG52_10590"
     CDS             1081..2364
                     /locus_tag="PLG52_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB41938.1"
                     /translation="MSKEHSRYRWFVVAVFFFFMLLHQTDKLMIGSLQVQISNDFNIN
                     NKQWGLVNSGALIVATILYPIWGYLYDRYSRTKLLSLASFIWGSTTWLNAIMKSFGGF
                     LVTRASTGIDDSSYPGLYTLIADYFGPTQRGKIFGILQLAQPIGYLLGMILALMVAPA
                     IGGWRSVFYITGSLGLVIALLIFFGVREMPRGKAEPEFENMTEMQTFKFSWAEAREIF
                     KKKTMWFVFLQGFAGVFPWNVITFFFFAYLEKERGYDANSILFTMAPVILILASGYFV
                     GGALGDYAFKRSNKGRIIVSSVGVLLGALFMYLAITTPLEAKNQFFVFMCLTAIFMPL
                     SSTNVTATVYDVTVPEVRSTAQAVEYFIENAGAAFAPIITGVIADMYDLKTAILLICT
                     VAWGLCFLFYLGAIFTIDKDANDLRSQMAERAKSM"
     gene            complement(2403..>2647)
                     /locus_tag="PLG52_10595"
     CDS             complement(2403..>2647)
                     /locus_tag="PLG52_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41939.1"
                     /translation="TTIPLYVTILSIFAHRDYGPAAAAHVLRGLLYGMFAFTGFFFIL
                     SLLIEETSLGVSFGLAVLTALAIQGTSLLILRRSHQ"
BASE COUNT          659 a          580 c          676 g          732 t
ORIGIN      
        1 ccttccaacg ggaatggggc aaacgcatca tgcgtttatc tcaggcgaag attccgactc
       61 tcctactaat cggcaatcac gacatttccc cgtccattgg gcgggctcac gctttgcaag
      121 agtttaaaac cctgcaagtg ccttatgtaa aagtgttgga tgctcctgag ttattaaagc
      181 cagaagacct ttgggatttg ccattgcaag ttattgccat gccgtgggtg gcacgctctg
      241 ggtttatggc agccactggt gagacggatt ccacgcaggc gttttcacgc attgaagaaa
      301 acattagtga gctaatcacc ggttggatcg aggaatgcga cctatctatg ccgattgtgt
      361 tgacggcaca tgcttccatt gaaggcgcaa agtttggcgg agaacgcttg gtaatgcttg
      421 gcaatgacct ggtgttgact ggcggattgg tgaaaaaccc gaagctcaac tatgtggcaa
      481 tgggtcatat ccacaagccg caagatgtga acgaaggaca tcaaccgccc gtgatttacc
      541 ccggctccat tgaacgtgtc gactttgggg aggcgaaaga agagcgcttc tttgtcattg
      601 cagatgtgga aaaaggacgc aacaccgaag tagagtggat tcaactcaca ggggtgagaa
      661 agtttataga ccgccgggca actatcgagt cgattgagag cgtgaccgag ttcctcaaga
      721 gcaaattgcc taagcctaag gaaatgtctg aggcgatcat ccgcttgatc gttgaatatc
      781 cacgtgaatg ggattcgttg atcgacgaat cagcgttgcg aaaatatgcc gccgatgcgt
      841 ttgagtttca ctttgtgaaa cgacctcaaa ttgagtcgcg agtgcgcatt gacgaagggc
      901 aggtggtgag cagccttagc ccactggatt tgcttgacca atattttgat gcggcaaaag
      961 tttcgaatgc agatgaattg aaaaacctgg cgcaggaaat catttcagaa gactcacaag
     1021 actcttgaca cttctacacg gacacctgat acttgggcat cccaacgtca tgaggttttt
     1081 atgtccaagg aacattcacg gtatcgctgg tttgtggtcg ctgtgttctt ctttttcatg
     1141 ctgttgcacc agaccgataa gttaatgatc ggttcactac aggtgcagat cagcaatgac
     1201 ttcaatatca acaacaaaca atgggggttg gttaattctg gagcattgat cgttgccacg
     1261 atcttgtacc cgatctgggg ctatttgtat gaccgatatt cacgcacaaa actgctttcg
     1321 ttagcatctt ttatttgggg ttctactaca tggttgaacg ccatcatgaa gagctttggc
     1381 ggattccttg ttacgcgtgc ctccaccggc attgatgact catcataccc gggcttgtat
     1441 accctaatcg ctgattattt cggacccact caacgcggaa agatctttgg tattttgcag
     1501 ttggctcaac ccattggcta tttgctgggt atgatccttg ccttgatggt ggctcctgcg
     1561 attggtgggt ggcgatctgt tttctatatc acaggctcgt tgggattggt gattgcctta
     1621 ttgatcttct ttggcgtgcg tgaaatgccg cggggtaaag ccgaacctga atttgagaat
     1681 atgacggaaa tgcaaacctt caagttctca tgggcagaag cgcgagaaat cttcaagaag
     1741 aagaccatgt ggtttgtttt cttgcagggc tttgctggtg tgttcccatg gaatgtgatt
     1801 acattcttct tctttgcata tcttgaaaaa gaacgcggtt acgatgctaa tagcatttta
     1861 tttaccatgg cgcctgtcat cttgattctt gccagcggat actttgtggg cggcgctttg
     1921 ggtgattatg ctttcaagcg ttcaaataag ggacgtatta ttgtctcctc tgttggtgtg
     1981 ttgcttggag cgttgttcat gtaccttgca atcaccacac cattagaagc caagaatcaa
     2041 ttttttgtct ttatgtgcct taccgccatc tttatgccac tctcttctac caatgtgacg
     2101 gcaacagtgt atgatgtgac ggttcctgaa gtgcggtcaa cggcgcaggc ggttgaatac
     2161 tttattgaaa atgccggggc tgcgtttgct cccatcatta ccggcgtgat tgccgatatg
     2221 tatgacctta agaccgctat tctcttgatc tgtactgtgg cttggggctt gtgcttcttg
     2281 ttctatctcg gcgcgatctt caccattgac aaagacgcga atgacctgcg tagccaaatg
     2341 gcagaacgcg caaaatctat gtaaaacgaa aacgggcttg gtgaaagaac caagcccgtt
     2401 ttttattgat gcgaccttcg taatatcagc agcgaggttc cttggattgc tagtgcagtt
     2461 agaacagcta aaccaaatga aacccctagg cttgtctctt caataagcaa gctgagaata
     2521 aagaagaaac cagtgaatgc aaacatgcca taaagcaacc cgcgcaagac gtgagccgca
     2581 gcggcgggtc cataatcacg atgggcgaaa atacttagga tggtgacata aagtgggatg
     2641 gtggtga
//
LOCUS       DAOQNJ010000394         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_29440_length_2640_cov_11.157834, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000394 DAOQNJ010000000
VERSION     DAOQNJ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29440_length_2640_cov_11.157834"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLG52_10600"
     CDS             complement(<1..362)
                     /locus_tag="PLG52_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HNB41940.1"
                     /translation="MKSITTPISDEAIRDLKVGDSVALSGMMVTGRDAAHKWMMETFI
                     KKTRAPQGDDLQVYEELKKLLNGSVIYHCGPVVSGLDTKEYKFLAAGPTTSIREEPYQ
                     ADVMKHFNVKGVIGKGGMG"
     gene            complement(381..1253)
                     /locus_tag="PLG52_10605"
     CDS             complement(381..1253)
                     /locus_tag="PLG52_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNB41941.1"
                     /translation="MRDLTNEILELIRRTSSSLPPDVEKRLKESIEKEEPGSAARGAL
                     ETIMKNIELSRSKSTPICQDTGTPIFQVHYPVGWSTIQLKKQIRAAMEEATKKSYMRP
                     NAVDAIYDKNTGNNLGGDDFPYIHFEENEGDELVIELMLKGGGCENVGRQYSLPDNGL
                     GAGRDLAGVRKVVLDAVQKAQGQGCAPGVLGVSIGGDRGSSYIKSKEVLFSEMGTRNE
                     DPKLAELEERLTSEANEMGIGPMGFGGKTTVLDTKIIGLSRLPASYFVSVSYMCWAYR
                     RRKMTVKGEEITYE"
     gene            complement(1355..2368)
                     /locus_tag="PLG52_10610"
     CDS             complement(1355..2368)
                     /locus_tag="PLG52_10610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB41942.1"
                     /translation="MDYTLLNDFSQIQAQEWNDLLKESISDTPFLRYEYQQAWWQHRG
                     GGEWKNAQLILVTARENGKLIGIAPLFIAEYENQPALLLNGSIEISDYLDLIVRKEDH
                     TNFLTGLLDFLASSLADNWSKLDWYNLPDDSPTLAALKAEGEKRGWAHLEEVYRPTPR
                     IALNGNFEDYLANIDKKQRHEIRRKMRRAAESELNVRFVIVDQSADIEPELRAFFDLM
                     VQDSNKATFLHPAMREQMTAFLRAAHAGGYLWLGFLEIAGEKTAASLNFDYNGKLWGY
                     NSGVSRSNMELSPGWVLMAHTIQWCCENGRYEFDFMRGDEDYKYRLGGVNRFVMRARL
                     NRK"
     gene            2421..>2640
                     /locus_tag="PLG52_10615"
     CDS             2421..>2640
                     /locus_tag="PLG52_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41943.1"
                     /translation="MPLDINLSPLYRVQGQEPTEMPGVLALQPPKNAARGREQDRLIV
                     YLALTGNATITTTEYRKLAEDVANVFYQT"
BASE COUNT          669 a          672 c          620 g          679 t
ORIGIN      
        1 cccatgccgc ctttaccgat cacgcctttg acgttgaaat gcttcataac atctgcttga
       61 tacggctctt cacggatgga agtggtgggt ccggctgcca aaaatttata ctcttttgta
      121 tccaaaccgc tgacaactgg tccgcaatgg tagatgaccg agccattgag caacttcttc
      181 aattcttcat acacttgcag atcatcacct tgaggggcac gagtcttctt gatgaaggtt
      241 tccatcatcc atttgtgagc ggcatcgcgc ccagtgacca tcatgccaga gagggcaact
      301 gagtccccta ctttcagatc gcgaatggct tcatcactga taggtgtagt aattgatttc
      361 atggttattc tccttacgat ttattcgtaa gtaatctctt cgcccttcac cgtcatcttg
      421 cggcggcgat atgcccaaca catataagac acagacacaa agtaggatgc aggcaaacgg
      481 ctcaagccga taatcttggt atcgagcacc gtcgttttgc cgccaaagcc cataggacca
      541 attcccatct cgttggcttc agaagtcagg cgttcttcaa gctcagcaag tttcgggtct
      601 tcgttgcgtg tgcccatttc actgaacaat acttctttag atttgatata ggaggaacca
      661 cgatcaccac caatggagac gcccaagacc cccggtgcac aaccctgacc ttgagccttc
      721 tgcaccgcgt cgagcaccac cttgcggacg cctgctaaat cacgcccagc cccgagtcca
      781 ttatctggca gggagtattg acgacccaca ttctcacagc cgccaccttt gagcatcaat
      841 tcgatgacga gttcatcgcc ttcgttttct tcaaaatgga tatacgggaa atcatcgccg
      901 ccgaggttgt tgccagtatt cttatcatag atggcatcca ccgcgtttgg acgcatgtag
      961 gatttcttcg tggcttcttc catcgcagcg cggatctgct tcttgagctg aatagtagac
     1021 caacccaccg ggtaatgcac ctgaaaaata ggtgtacccg tatcctgaca gatcggcgtg
     1081 gacttagaac gggaaagctc aatgttcttc ataatcgttt caagcgcacc acgtgctgcc
     1141 gaacccggct cttctttttc aatggattct ttcaagcgtt tttcaacatc aggcggcaaa
     1201 gagctggatg tgcgccgaat gagttctaaa atttcgttgg ttaaatctcg catgggtacc
     1261 tccaatacag aattgaatct tactcccgaa aaggggaatt tgccacataa aaacggagcc
     1321 ctgcgtattt tctaacgctg gactccgtaa aaaattactt tctattaagt ctggctcgca
     1381 tcacaaagcg atttacgccg ccgaggcggt atttgtaatc ttcatcgccg cgcatgaagt
     1441 cgaattcata acgtccgttt tcgcaacacc attgaatggt atgagccatc aacacccagc
     1501 ctggcgaaag ctccatattc gaacggctaa caccagagtt gtagccccag agttttccgt
     1561 tgtaatcaaa gttcaaagaa gcagcggtct tttcaccggc aatctctaag aagccaagcc
     1621 agagatagcc acctgcatga gcagcgcgca agaaggcggt catttgctcg cgcatggcag
     1681 ggtgtaaaaa ggttgctttg tttgagtctt gcaccatcag gtcgaaaaag gcgcgaagtt
     1741 caggctcgat atctgcagat tgatctacaa tgacaaagcg aacattgagc tcagactcag
     1801 ccgcgcggcg catcttgcga cgaatttcat ggcgctgttt tttgtcgatg ttggcgagat
     1861 aatcttcaaa gttgccgttg agggcaatgc gtggagtagg gcgatacact tcttcaagat
     1921 gagcccagcc gcgtttttcg ccttcggctt tgagggcggc gagagtgggc gaatcatcag
     1981 ggaggttata ccaatcgagt ttggaccagt tatcggctag ggaagaggcg aggaagtcta
     2041 atagccccgt gagaaaattt gtatggtcct ctttgcggac gatgagatcc agatagtccg
     2101 aaatttcaat actgccattg aggagcaaag caggctggtt ttcatattct gcaatgaaaa
     2161 gcggagcaat gccaatgagt ttgccatttt cacgagctgt gacgagaatc agttgagcat
     2221 tcttccactc accaccacca cggtgttgcc accatgcctg ttggtattcg tatcgaagaa
     2281 agggcgtgtc gctaatggac tctttgagaa ggtcgttcca ttcttgcgcc tgaatttgag
     2341 agaaatcgtt gagaagtgta tagtccatag ccgtcgaatt ttaccagtta taatgaaggc
     2401 gacttaagcg attatcttac atgccactcg acatcaatct ttcaccttta tatcgtgttc
     2461 aggggcaaga acccactgaa atgcccggcg tactggcgct gcaaccaccc aaaaacgccg
     2521 ctcgtgggcg cgaacaagac cgactgatcg tatatttggc gctcaccggc aatgccacca
     2581 tcactacaac tgaatatcgc aaactcgctg aagatgttgc gaatgtgttc tatcaaactc
//
LOCUS       DAOQNJ010000395         2638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_29486_length_2638_cov_11.120015, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000395 DAOQNJ010000000
VERSION     DAOQNJ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29486_length_2638_cov_11.120015"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLG52_10620"
     CDS             <1..210
                     /locus_tag="PLG52_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41944.1"
                     /translation="AQEIMDDLSHEGELQKSEVKQWVDQLSDRGEEERQALRKLIRDE
                     MKKVLDDMGLATKEDIQKILEQRDS"
     gene            332..2008
                     /locus_tag="PLG52_10625"
     CDS             332..2008
                     /locus_tag="PLG52_10625"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNB41945.1"
                     /translation="MFAPSRSIRHLQRYSEIVSVFARHGFGFLFHRPDSRRRPLFRGP
                     WRTDQTGMPPHVDNLAVHFRTALEELGPTFIKFGQILSTRPDLLPLSFITELSKLVDS
                     VSPEPWEVIHASLTRELGKDPEKLFTSIDPHPMASASLSQVHAGVLLNGQEVVIKVQR
                     PNITSVIDTDLEIMASLAASAQSTAMGKIYDFAGIADDFSFTLRNELDYQREGRNADR
                     LRESFAGQENRLYIPKIYWELSTQRVLVMERIYGIKISDIPALNAAGYNRHKVALNSA
                     SVTIKQVLEDGFFHADPHAGNYLIMPGEAIGLVDFGKVGYLRDKDRMDLIRLYIVAVE
                     MDVDGLIDQMMRIGAVREDVDRNRLAYDLNRFLNKYYGVPIKYVRAKELIDEITTITF
                     RHRLRLPTTWWLVGQTIVMLEGIGLQLDPDFDVFQAAEPHMQRLMKNLFLPNGGWARN
                     VLMDATNWGEVLHRLPRVGNRMLERLEKNEPFRMEVKDTDRILAKLDRLVTRLSLSLL
                     VAAFVVGLPLLIPLTTPDSLPRWLLLVGMVPIIGTGVWLGFSLLNTPKNK"
     gene            2152..2472
                     /locus_tag="PLG52_10630"
     CDS             2152..2472
                     /locus_tag="PLG52_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41946.1"
                     /translation="MAKVKLNPALQQIRGRLAGFVYRIRYGEQTISKSPDMSKVKWSA
                     AQVEHRRRFKLAVAYAHLAMGDEKVRLQYMAEAAEKGKRPFDLAVSDYFKGRNLLMKS
                     ESSK"
BASE COUNT          664 a          614 c          692 g          668 t
ORIGIN      
        1 gcacaagaga tcatggatga cctcagccat gaaggcgagc ttcagaagag cgaagtcaaa
       61 cagtgggttg accagctttc cgaccgcggc gaagaggaac gacaggcttt gcgcaagttg
      121 atccgtgacg aaatgaaaaa agtattggat gacatgggtc tggcaaccaa ggaagatatt
      181 caaaaaatcc ttgagcagcg agattcatag tctgcttcaa ttttcctgcc cttgaaatac
      241 cggatttgct caatgagcca agcctttgag ttttggtatt ttgtgtatgg acattgataa
      301 tggcctctaa tctttatcgg aatgggtttt gatgtttgcc ccctcacgtt ccattcgtca
      361 tttacaacgg tacagtgaga tcgtatctgt atttgcccgc catgggtttg gtttcttatt
      421 tcaccgccca gactcgagac ggcgtccttt gtttcggggt ccgtggcgga cagaccaaac
      481 tggcatgccg cctcatgtag ataaccttgc agttcacttt cggacagcat tggaagagtt
      541 ggggcctaca ttcataaaat ttggacagat cctttccact cgtccagatc tgctccccct
      601 ttcttttatt acagaactaa gtaaactcgt cgactcggtc tcgccggagc cctgggaggt
      661 gattcacgcc tcgttgactc gtgagctggg caaagaccca gagaagttgt tcacctccat
      721 tgatccgcat cccatggctt cggcttcact gtcacaggtc catgcggggg tattgttgaa
      781 tggacaggaa gtggttatca aggttcaacg cccgaacatc acttcagtga tcgataccga
      841 tcttgagatc atggcgtctc ttgctgcgag tgcgcaatcc actgcaatgg gaaaaattta
      901 cgatttcgcg ggcatagccg atgatttcag tttcaccctg cgaaatgaat tggattatca
      961 acgcgaaggc cgcaatgccg accgcttgcg cgagagcttt gccgggcagg aaaatcgcct
     1021 ttacatccca aagatatatt gggagttgag cacccagcgt gtgttggtca tggagcggat
     1081 ttacggcatc aagatcagtg atatccccgc cttgaatgcc gctggatata accgtcacaa
     1141 ggtcgccttg aatagtgcca gtgtgaccat caaacaggtg ctggaagacg gcttcttcca
     1201 cgccgacccc catgccggga attatctcat catgccgggc gaggcgatcg gtctcgtgga
     1261 ttttggcaag gtgggttacc tgcgtgataa agaccgcatg gatttgatcc ggctttacat
     1321 cgttgccgtc gaaatggatg tggacggcct catcgaccaa atgatgcgca tcggcgctgt
     1381 gcgggaggat gttgaccgta accgactggc ttatgacctc aatcgttttc taaacaagta
     1441 ttatggtgta cccatcaagt atgttcgcgc aaaggaattg atcgacgaga tcacaacgat
     1501 cacttttcgg catcggttgc gcctgccgac cacctggtgg ctggtggggc agaccattgt
     1561 gatgttggaa ggcattggtt tgcaattaga cccggacttt gatgtctttc aagccgcaga
     1621 accgcacatg cagcgcttga tgaaaaatct tttccttcca aacggcggat gggcccgcaa
     1681 tgttctcatg gatgccacca attggggcga ggttctgcac cgcttgccgc gtgttggaaa
     1741 ccgcatgttg gaacgcctag agaagaacga gcccttccgc atggaagtga aagacacaga
     1801 ccgcattctg gcaaaattag accgactggt tacgcgtctt tctttgagtt tgcttgttgc
     1861 tgcttttgtg gtgggtctgc cgctgttaat tccgctcact actcctgata gcctgccaag
     1921 gtggttgctt ttggttggca tggttccgat cataggcact ggagtctggc tgggcttttc
     1981 cttattgaac actccaaaaa acaaataaca acctagtaaa ccagaaaacg cccccaatga
     2041 cttggaaagg tgagcgtgat tctggggctc atacttggac tataactaga cttatactac
     2101 gggatcagta caggattagt acaggattat gggtgagacc atggaggaaa tatggcaaaa
     2161 gtgaaattaa acccggctct tcaacaaatc cgcggacgat tggcaggatt tgtgtaccga
     2221 atacgatatg gagagcagac catttcgaaa tcgcctgata tgtccaaagt gaagtggagc
     2281 gcggcacagg tggagcaccg acgccgcttt aagcttgcag tagcctacgc acacctggca
     2341 atgggcgatg agaaagtaag gctgcaatac atggcagaag ctgctgaaaa gggcaagcgt
     2401 ccatttgacc tggcggtctc ggattatttt aaagggagaa atttattgat gaagagcgaa
     2461 agtagtaagt gatttgatat aactcacctt aggcgatgtc gttctgagcg gagcgaagaa
     2521 cctctacgat tatccaagaa acccttcgct atcgctcagg gagacacaaa aaagaaaaca
     2581 cctctcccta tgtcttgaga ggtgatgttt tattatttgc caaagaggtg acagtcag
//
LOCUS       DAOQNJ010000396         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_29675_length_2629_cov_7.362082, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000396 DAOQNJ010000000
VERSION     DAOQNJ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29675_length_2629_cov_7.362082"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..982)
                     /gene="mreC"
                     /locus_tag="PLG52_10635"
     CDS             complement(140..982)
                     /gene="mreC"
                     /locus_tag="PLG52_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNB41947.1"
                     /translation="MNSRSLQTTIIFLVIGGILVLAFSGALGSASRGFTSVLVDVQTW
                     ISSRFLGFQDFVTAPRDIVTLRTRNAELEAQVSQLQAQLIELQQRVNETEILAALVDF
                     SRSNPESTYKAASVIGRDPSPFLHYIIINRGSNDDIRRGMPVVTNQGLVGRVDAVIAD
                     AARVQLITDPSSSVNVYLQNADTSAVLYGSVTGDVSLDLISQNASVEAGDLILTSGLG
                     GGYPADLLIGQVVTVRSLEFELFQQATVQPAVDFSRLEIVLVITNFRPVDIAPLEPVV
                     TPSP"
     gene            complement(1019..2086)
                     /locus_tag="PLG52_10640"
     CDS             complement(1019..2086)
                     /locus_tag="PLG52_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNB41948.1"
                     /translation="MAFNPINWLLGLFSLDIAIDLGTANTLVHVRGKGIVINEPSWVA
                     VDKKQRQPLAIGLEAKEMVGRTPANVVVVRPIRDGVIAEFDVTRVMLEYFIGKVHEQS
                     VVPLPRPRVIVGLPTGVTEVEKRAVYDAVMASGARQAMLIEEPIAAAIGAGLPIGEVR
                     GSMVVDIGGGTTEVAVMSTGGVVASRSLRVAGDEMDQDVVQYLRNKYNLLVGTGIAEQ
                     IKWQIGSAYPLQPEKTMEVRGRNLVTGLPETVEISSVEIREALSGSVQVIIDTVRDAL
                     DEIPPEIVSDLMDIGICLAGGGALLQGLAERLTDELKLRVWVAEDPLTCVARGAATVF
                     EDLESLSRYLIGLERGSTRHV"
     gene            2226..>2629
                     /locus_tag="PLG52_10645"
     CDS             2226..>2629
                     /locus_tag="PLG52_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNB41949.1"
                     /translation="MSKFAIVTDSTSYIPTDVAQKHGITIAPQVLIWDNQTYRDGIDI
                     QPTDFYSRLKTAKSMPSTSQVSPATMQEIFQGLVEKGTSVLGIFISSKLSGTLQSAIQ
                     AKDMMGSAGEKVTLVDSRSTAMGLGFQAIIAAR"
BASE COUNT          766 a          677 c          576 g          610 t
ORIGIN      
        1 cttgtagtgc ccaagccgcc aacaatacga gcggcaggtc agcgtaccct gagagcagcc
       61 ttacttgact gacagccgca gattgcagaa taactgccaa tcctattaac ggaaatgcga
      121 ttaaattgcg catgaaaaat tacggggaag gtgtaaccac gggctcaagc ggagcaatat
      181 ccactgggcg gaagttggtg atgacaagga cgatttccaa gcgagaaaag tcaacggcag
      241 gttgaactgt cgcttgttga aacagctcaa attccaaaga gcgaaccgtg acgacttgtc
      301 cgatcaggag gtctgctggg tatcctccac ccaaccctga ggtgagaatc aaatcgcctg
      361 cttctacaga ggcattttga gagattaggt caagagaaac atcgccagtg accgaaccat
      421 acaaaacagc gcttgtatct gcattttgca aatatacatt gaccgaagaa gatggatctg
      481 tgattaactg aacgcgggca gcatctgcaa tgaccgcatc aacacgcccc acgagacctt
      541 gattggtcac aacgggcata ccacgccgaa tatcatcatt cgaaccgcgg ttgatgatga
      601 tgtaatgcaa aaatgggctt gggtcacgac cgatgaccga tgcagcttta tatgtacttt
      661 cagggtttga acgggaaaaa tcaacaagag ccgcaagaat ttcggtctca tttacacgtt
      721 gttgcaattc aatgagttgt gcttgcaatt gagaaacttg cgcttccaac tctgcattac
      781 gcgtacgtaa tgtgacgata tcgcgtggcg cagtgacaaa atcttgaaag ccaagaaaac
      841 ggctggatat ccacgtttgg acatcaacaa gcacagaagt aaatcctctg gaagctgaac
      901 ctaatgcgcc gctaaaggca agcactagaa tccccccaat caccagaaaa ataattgtcg
      961 tttgtaagga acgagagttc attttttatt cagcccacga aaggctgtct acttaatcct
     1021 atacgtgacg cgtactgcca cgttcgagac cgatcaaata gcgagaaagg ctctcaaggt
     1081 cttcgaatac agtggctgca ccacgtgcta cacaagtaag tgggtcttca gcaacccata
     1141 cacgtaattt cagttcatcg gttaaacgtt ctgccaagcc ttgcaagagc gcgccgccgc
     1201 cggctaaaca aataccaata tccattaagt ctgaaacgat ttcgggcgga atttcatcca
     1261 aggcatcgcg aacggtatca atgatcactt gcaccgaacc agagagcgct tcgcgaattt
     1321 caacagaaga aatttcaacc gtttcaggta aaccagtcac taaatttctg ccgcgcactt
     1381 ccatggtttt ttcaggttgc aatggatatg ccgacccaat ttgccatttg atctgttcag
     1441 caatccccgt ccccactaat aaattgtatt tgttgcgcaa atactgcaca acatcttgat
     1501 ccatttcatc accagccacg cgcaatgaac gtgacgccac tacaccaccc gtagacatga
     1561 ctgcgacttc agttgtgccg ccaccaatgt caacgaccat agaaccgcgg acttctccaa
     1621 ttggaagacc ggctcctatc gccgccgcaa ttggttcttc aatcaacata gcttggcgcg
     1681 cgcctgacgc cattactgca tcatacacag cgcgcttttc cacttcggtc acaccggtag
     1741 gtaaaccaac gatcacacgc ggacgtggca aaggcacaac actctgttca tgtacctttc
     1801 caataaaata ctcaagcatc acacgtgtta catcgaattc tgcaatcacc ccatcacgaa
     1861 taggacgaac caccaccaca ttggcgggag tacgccctac catttccttg gcttcaagac
     1921 cgatcgcaag aggctggcgt tgtttcttat caacggctac ccaagacggt tcattgatca
     1981 caataccctt accacgaaca tggacaagag tatttgcggt gccaaggtca atggcaatat
     2041 ccaaagaaaa aaggcctaac agccagttaa ttgggttgaa ggccaaaatc ggctcctatg
     2101 atacgagaat gggtctaagc aaaactgccg agccattata ccatgaggat gagtccgaat
     2161 attttttaat tctgttaccc aaacggggta aaatcaggaa aatttttccc cgtggaggaa
     2221 gagaaatgtc aaagtttgcc attgttacag atagcacttc ttatattcca accgatgtcg
     2281 cccagaaaca tggaattacc attgccccac aagttctcat ttgggacaac caaacatacc
     2341 gcgacggtat tgatattcag cccaccgact tctacagccg cctcaagacc gccaagtcga
     2401 tgccgtcaac ttcgcaggtt tcccctgcca ccatgcagga gatatttcag gggctggtcg
     2461 aaaaagggac ttctgtgctt ggcatcttca tctcttcaaa attatcaggc acattgcaat
     2521 ctgccattca agccaaggat atgatgggaa gtgctggcga aaaagtaacc ttagttgaca
     2581 gccgctccac tgcaatggga cttggttttc aggcaattat tgctgctcg
//
LOCUS       DAOQNJ010000397         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_29729_length_2626_cov_10.185531, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000397 DAOQNJ010000000
VERSION     DAOQNJ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29729_length_2626_cov_10.185531"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PLG52_10650"
     CDS             complement(<1..685)
                     /locus_tag="PLG52_10650"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNB41950.1"
                     /translation="MSTPRVIRAPRGTELTCKNWLSEAAYRMIQNNLDPEVAEKPEDL
                     VVYGGRGKAARTWEAYDAILESLKNLEEDETLLVQSGKPVVIFKSHKDAPKVLIANSN
                     IVPHWATWEYFDELAKKGLIMYGQMTAGSWIYIGTQGILQGTYETFGALAKLKGWGSL
                     KGKFVLTAGLGGMGGAQPLSITMNEGVGLIVEVDPERAERRRAIGYVDMVVDNLEEAM
                     TLVEENVKNG"
     gene            complement(698..1549)
                     /locus_tag="PLG52_10655"
     CDS             complement(698..1549)
                     /locus_tag="PLG52_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41951.1"
                     /translation="MKQHRGFLMAVLLMLTALACVVPGLQPASAIPPTPDTRLDRMVA
                     ETVAAALALTQQVPTATNTSTPTPTSTPTSTPLPVEPTQAEESVLDKNTDGTSSFIDL
                     SGKYQFTVPTQWLALRINSPEYDEAVLLPEAANPAIQNQLSVIQKQDPNVFRLFLLDI
                     NEEHIDSGFVTNVNLVWDPQMEISVATDSDLKGLAATLPTSLKDAEVTGVEIKTTTGG
                     LSYGVITATTPLMTQEGARLNVYQKLVFFDLPTGTLSVTLSTTEKWQSTVEPFFDEFV
                     TSFLILE"
     gene            complement(1640..2053)
                     /locus_tag="PLG52_10660"
     CDS             complement(1640..2053)
                     /locus_tag="PLG52_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNB41952.1"
                     /translation="MQITRQADYAVRAVLHLARNGETRTATSLIAEEQKIPPSFLAKI
                     ISQLSIAGLLHTSRGARGGVTLAREPKDITLLEVIEAIDGPIQLNECVGDTGTCSFDD
                     NCPLRPVWCEAQEQLVGRLKGTNFADMIAKGQTAA"
     gene            complement(2243..>2626)
                     /locus_tag="PLG52_10665"
     CDS             complement(2243..>2626)
                     /locus_tag="PLG52_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41953.1"
                     /translation="ELAFSEPNPVEMSTFTYSTGASDVKITGLANANFSFFDFSSGAG
                     DYTLDFSGDLQRDASVKIETGLSNFIIIVPEGVDAVVTVQGGLSNVNAGPGWERSGSD
                     YIQKGEGPTITFVIEMGAGNLTLAR"
BASE COUNT          645 a          637 c          667 g          677 t
ORIGIN      
        1 tgccgttctt gacattctct tcaaccaagg tcatggcttc ttcgaggtta tccaccacca
       61 tgtccacata gccaatggca cgacgacgct ctgcacgttc tgggtccact tcaacaatga
      121 gaccgacacc ttcattcatg gtaatggaaa gcggttgagc accacccatg ccacccagcc
      181 ctgcagtgag cacaaacttc cctttgagtg agccccagcc cttaagtttg gcaagtgcac
      241 caaaggtttc gtaggtgcct tgcaaaatgc cttgtgtgcc aatatagatc cacgaaccgg
      301 cggtcatttg cccgtacatg atgagaccct ttttggcgag ttcatcgaag tattcccagg
      361 ttgcccaatg cggcacgata ttggagtttg cgattaatac tttcggcgca tctttatgag
      421 acttaaagat caccaccggc ttaccagact gcaccaacaa ggtctcatct tcttcgagat
      481 ttttcagcga ttccaaaatc gcgtcatacg cttcccaagt ccgcgcagcc ttaccccgcc
      541 caccgtagac gacaaggtct tctggctttt ctgccacttc ggggtcaagg ttgttctgga
      601 tcatacgata ggcagcttcg ctgagccagt tcttgcaagt cagttcggtg ccgcgtggcg
      661 cgcggataac acggggtgtg gacatgttta gtctccgtta ttccaatatg aggaaagagg
      721 taacgaattc atcaaagaaa ggttcaactg tgctttgcca cttttcagtg gtggaaagag
      781 tcacagataa ggttccggta ggcaaatcaa aaaacaccaa tttttgatac acattcaacc
      841 gggcgccttc ttgggtcatg agaggcgtgg tggcagtgat aacgccatac gataaaccgc
      901 cggttgtagt tttgatctca acaccggtca cttcggcatc tttgagagac gtgggcagag
      961 tagccgccaa acctttgagg tctgaatcgg tggcgaccga aatttccatt tgcgggtccc
     1021 ataccaaatt gacgttggtg acaaagccgc tgtcgatatg ttcttcattg atatcaagca
     1081 agaaaagacg gaagacattc gggtcttgtt tttggatgac cgagagttga ttttgaattg
     1141 cggggtttgc cgcttcgggc aacaacacag cttcatcata ttccggtgag ttgatgcgca
     1201 acgcaagcca ctgtgtggga accgtaaatt gatacttacc gctcaagtca atgaaagagc
     1261 tggtgccatc agtgttctta tccaagacac tttcttcggc ttgggttggc tcaacaggca
     1321 gcggcgtgct tgtgggtgtg ctggttgggg tcggggttga agtattggtg gctgtaggaa
     1381 cttgttgcgt taacgcgaga gcggcggcaa cggtctcagc gaccatgcgg tcaagtcgtg
     1441 tatcaggtgt cggcggaatc gcagaggcgg gttgtaatcc cggcacgaca catgccaaag
     1501 cagtcagcat gagtaagact gccatcaaaa atccacggtg ttgtttcata ggtttatcct
     1561 tgcaaatgga attacccata gggtatataa aaaaaagcct cccggctatt atagccgaga
     1621 ggctttgatt tctttgacct tacgccgcag tttgaccttt ggcgatcata tctgcaaagt
     1681 tggtgccttt caagcgacca accaactgtt cttgtgcttc gcaccatacc gggcgcagcg
     1741 gacagttatc gtcaaaggag caagttccgg tatcgcctac acattcattg agctgaatgg
     1801 gaccgtcgat agcttcgatg acttcaagca gagtaatatc tttcggttca cgcgcaaggg
     1861 ttacgccgcc gcgcgctccg cgggaggtgt gcaacaaccc ggcaatcgaa agttgagaaa
     1921 tgatcttcgc aaggaaggag ggtggaattt tttgttcttc agcgattaag ctggtggcag
     1981 tgcgggtttc gccattgcga gcaagatgga gcacagcgcg tacagcataa tcggcttgtc
     2041 gggttatttg cataaggacc tcacttaata taggtaaaac ttacccacaa tcacgatttt
     2101 attgtgaacg gcgttacaag gcaagtgata gaggtcactg atgaattatg acctttcgcg
     2161 cttaattaga aagatggttt ttaaacgaaa aacggattac ttgtaaggta atccgttttt
     2221 tttcaacttg taaaattctt tcctaacgtg ccaaggtcag attccctgcc cccatctcga
     2281 tgacaaaggt gatggttgga ccttctcctt tttggatgta atcgcttccg cttctctccc
     2341 agccgggacc tgcattcaca ttcgacagcc cgccctgcac tgtgaccact gcgtcgaccc
     2401 cttcgggcac aatgatgatg aaattgctta agccggtctc gatcttgacc gaagcatcgc
     2461 gctgcaagtc accggagaaa tcaagagtgt agtcacctgc gccggaagaa aagtcgaaga
     2521 agctaaagtt ggcatttgcc agcccagtga tcttcacatc cgaagcgcca gtgctatagg
     2581 taaaggtgct catctcaacg ggatttggtt cagagaaagc gagttc
//
LOCUS       DAOQNJ010000398         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_29730_length_2626_cov_8.609102, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000398 DAOQNJ010000000
VERSION     DAOQNJ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29730_length_2626_cov_8.609102"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..308)
                     /gene="ffs"
                     /locus_tag="PLG52_10670"
     ncRNA           complement(210..308)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLG52_10670"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(350..1732)
                     /locus_tag="PLG52_10675"
     CDS             complement(350..1732)
                     /locus_tag="PLG52_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB41954.1"
                     /translation="MSNDIIHILLVEDEDPHAELIQRAFEDQGSEFQIHRVRSLTEAR
                     EHRRLNEPKLIIADWRLPDGESLELLPNHRDPLATPIILMTSYGNERIAVEALKSGAL
                     DYVVKSPESMLDMPHLARKALDQWKSRADRIHMQEALTESEAHFRLLAENASDMITRI
                     NTNGQMFYISPACKTILGYQPEEMLGVKVFDLINPEDQPQIRELFSQKPEDSPYTITY
                     RAKHKSGHYVWLETSARAIIDHSTDEVIEIQAASRDITERKRAEEALQLAHNQLRDAY
                     ERTIEGWVRALDLRDRETEGHTQRVTDITIRVAQQLGFSEEELSHIKRGALLHDMGKI
                     AIPDEILQKPGPLNETEWERMRQHPMYAYEMLSPIAYLNPALDIPFYHHERWNGSGYP
                     HGLKGEKIPLSARLFAIVDVWDALRSDRPYRKAIPREQVIEYLSENANILFDPKLVEV
                     FLKFVEEHIP"
     gene            complement(1739..2182)
                     /locus_tag="PLG52_10680"
     CDS             complement(1739..2182)
                     /locus_tag="PLG52_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB41955.1"
                     /translation="MLGEPILVMLVEDNADHAELVIRTMESHHIPTRIQHVTDGESAL
                     DYLLRRNNFSDPETSPRPHMILLDLRLPRVDGLEVLRIIKEEEDLKSIPVVILTTSEA
                     EKDVMHAYEHYVNSYLVKPVGFEDFSSLMKDLGIYWLGWNKQLRA"
     gene            2355..>2626
                     /locus_tag="PLG52_10685"
     CDS             2355..>2626
                     /locus_tag="PLG52_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB41956.1"
                     /translation="MTESALLNDRYQLDELLGSGGMANVYRARDVVLDRPVAIKVLRK
                     EYSTNENFQKQFWLEARSAANLSHPNIVTVHDFGAADDLLYIVMELV"
BASE COUNT          655 a          606 c          641 g          724 t
ORIGIN      
        1 gcttcggcgg tcatgagcac ataatctaca cggtaatctt cgcgcagaac atctggcgtt
       61 acatctgtat attccactgg gctgagcacc cagccataca caagtccaag cccagtgcct
      121 atggcaagtg caatgaggat cgttataatt tttgatgaag acatgtggaa attatactgc
      181 tttgagaatt ttgacccact ataaaaatat ccgagcaggc gaccggcggc acccgaagac
      241 tcttccttac cgttgctatc tttcgatcct ggcggggttc gaaaggcttc gccgcgccgg
      301 gcctgcccgg acaggcgcaa ggatacccga tgatgagagg ggtgtcaaat cacggaatgt
      361 gttcttctac gaatttcaag aaaacttcta ccagtttggg gtcgaacaga atgttggcat
      421 tttcgctgag atattcaatg acttgctcac gggggatcgc tttgcgatac gggcggtcac
      481 tgcggagcgc atcccataca tctacaatgg cgaatagacg ggcagaaagc ggaatttttt
      541 caccttttaa tccatgagga taaccgcttc cgttccagcg ttcgtgatga tagaagggaa
      601 tatctaaggc ggggtttaag tatgcaatgg gggagagcat ctcatacgca tacatggggt
      661 gctgccgcat gcgttcccat tccgtctcat tgagcggacc aggcttttga agaatttcat
      721 cggggatggc gatctttccc atatcgtgga gcaatgcgcc tcgtttaata tgggagagtt
      781 cttcttcgct gaacccaagc tgttgtgcaa ctcgaatcgt gatatcggta acgcgctgcg
      841 tgtgaccttc tgtttcgcgg tcacgcaagt ctaatgcgcg gacccaacct tcaatggtgc
      901 gttcgtaggc atctcgaagc tgattgtgtg ccagttgcag tgcttcttct gctcgcttgc
      961 gttctgtaat gtcacgtgat gcagcttgaa tttcaatgac ttcatctgtg ctgtgatcga
     1021 tgatggcacg tgcggaggtt tccaaccaaa cgtaatgtcc gcttttgtgt ttggcacgat
     1081 aggtgatggt gtatggggaa tcttcaggtt tttgagaaaa aagctctctg atctgaggct
     1141 gatcttcggg gttaataagg tcaaatactt tgacccctaa catttcttca ggctgatatc
     1201 caagaatggt tttacaagcc ggtgaaatat aaaacatttg cccgtttgta ttgatgcgag
     1261 tgatcatgtc actggcgttc tcagccagca agcgaaagtg agcttcactc tctgtaaggg
     1321 cttcctgcat gtgaatgcgg tctgcccgag acttccattg gtccagtgct tttcgtgcca
     1381 agtgaggcat atccagcatt gattcagggg acttcaccac ataatctaac gcaccagact
     1441 ttaacgcctc taccgcgatg cgttcattgc cataacttgt cattagaatg atgggagtag
     1501 ccagcgggtc gcggtgattg gggagcaatt ccaagctttc cccatctggg agccgccaat
     1561 ctgcgatgat aagtttgggt tcgttcaagc gtctatgttc gcgggcttct gtaagagaac
     1621 gcacacggtg aatctgaaac tcagacccct ggtcttcaaa agcgcgctga atcagctccg
     1681 catggggatc ttcgtcttca actaaaagaa tatgaatgat gtcattcgac atgagggatt
     1741 aagcgcgtag ttgtttattc caacccaacc agtaaatgcc aaggtctttc attaagctgc
     1801 tgaaatcttc aaacccaacc ggctttacta gatagctgtt gacataatgt tcatacgcgt
     1861 gcataacgtc tttttcagct tcagacgttg ttagaataac aacaggaatg ctcttaaggt
     1921 cttcttcttc tttgatgatt cgcagtactt ccaacccatc aacacggggt aagcgtaagt
     1981 ccaaaaggat catatgtggg cgagggctgg tttcagggtc actaaagttg ttgcgacgca
     2041 ggaggtaatc caatgctgat tcgccatcgg ttacatgttg aatccgtgta ggaatgtgat
     2101 gactctccat ggttcggatg acaagttctg catggtcagc attatcttca acgagcatga
     2161 caaggattgg ttcgcctagc atggtggatc tcctaaacag tcatattgta aacgatttac
     2221 gaataacggg tgaaaatcgt tatgacaact ttatttaaca gacctgttaa gacaactaaa
     2281 catataaact ccccaaaata tcgattttct atctccgtgc ctctatgtag aactcacggt
     2341 ataatctcac cacaatgacc gaaagtgcat tactaaacga ccgatatcaa cttgacgaac
     2401 ttctcggctc tggcggcatg gcgaacgtgt atcgcgcgcg agatgtggtg ctagaccgtc
     2461 ctgttgcgat taaggtctta cgcaaagaat attccaccaa cgagaatttt caaaaacaat
     2521 tctggctgga agcgcgttcc gcggcgaatc tttcgcaccc caatattgtc accgttcatg
     2581 attttggcgc tgccgatgat ttgctctaca tcgtcatgga acttgt
//
LOCUS       DAOQNJ010000399         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_29731_length_2626_cov_8.531311, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000399 DAOQNJ010000000
VERSION     DAOQNJ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29731_length_2626_cov_8.531311"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2524
                     /locus_tag="PLG52_10690"
     CDS             <1..2524
                     /locus_tag="PLG52_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNB41957.1"
                     /translation="LTDSRATPEDWQSALSLYTGDLLPQVYEDWVLEWRAEFEQACLE
                     TRLRLAQHQRGQGQYVAAIETARLALEIEPVRESTHQLIILCYESLGDREAALLQYES
                     CSKALREIGVEPSPELQGIYRRLQKTPASLHITGNLPRPLTSFIGREEELNEVETLLA
                     ETRFLTLLGAGGSGKTRLAIQAADESAHNYPDGVWWVDLAPLLKGDQLPQAIAKSLGV
                     KEKLGADMLDHIASFIGERKMLIILDNCEHLVSATAHSIEAVSAKCQAVTFLATSREP
                     LGVASEIGWQTPAFPLPEPGASHLKTLAKNESVRLFVERGRAVNGSFQLNGQNVMRVA
                     EICRRLEGMPLAIELAAAQVNNLTLDGILSRLEKSLNLTSTDAAHARHAALHSAIEWS
                     YNLLTPAEQMLFRRLAIFSGGWNLASAAIVAAGYSDPEAPLSNEASEGTIAPLPINGE
                     TVTRQILDALVKKALAQFRRAEGSLRYDTLDTLREFAREKMNEVGETQIMQERHALTF
                     FNFLKKAWDDVYSERESSIFNQIDLEYANLRTAWAWQLQSPKWVVDAKAINSQNRFWY
                     VRGHYAEGRETLEALLKHPDVHLNTLIHATLLNNLGLIEWKSGNRIAAKRIFEEALVE
                     FRTLQLDFAAAMIIANLAGLYIDDDDMEKGLPLAHEAVVVARETGNKSALNISLNNLA
                     IALTITRDFKGAREAFYEGLALSKEQGDIRGEGIIYNGLADIEAEEGNFAEARRLFTQ
                     SFMTRSSLGDRRGSIIPALNLIRLMCMDEDWENAACLLGFVEETLQQLGIQLASDARA
                     GREEANRSVRLALDEETVTRALQFGKTLTLDSANRWLAEKSL"
BASE COUNT          708 a          646 c          630 g          642 t
ORIGIN      
        1 cctaactgac tcacgtgcta cgcccgaaga ttggcaatct gcactgagtt tatatactgg
       61 ggacctactt ccacaagttt atgaagattg ggtattggaa tggcgggcag aatttgaaca
      121 agcttgcctt gaaacccgtt tacgccttgc tcaacatcaa agaggtcagg gtcagtatgt
      181 ggctgcaatt gaaaccgctc gccttgcgct agagattgaa cccgtccgtg aatccacgca
      241 tcaattgatc atcctctgtt atgagtcact tggagatcgg gaagcagcgc tcttacaata
      301 cgaatcgtgc agtaaagctc tacgagaaat tggcgtagag ccatcgccag agttacaagg
      361 tatttatcgc cgcctgcaaa agacacctgc atctttgcat ataacaggca acctcccccg
      421 cccattgacc agctttattg ggcgcgaaga agaactgaat gaagtagaaa ccttattagc
      481 agaaactcgc ttccttacct tattgggtgc aggtggaagt ggcaagaccc ggcttgccat
      541 tcaagcagct gatgagtctg cacataacta tcctgacggg gtctggtggg ttgaccttgc
      601 tcccctgctc aaaggagacc aattgccgca agccattgcg aaatcgcttg gggtaaaaga
      661 aaagctcggc gccgatatgc ttgaccacat tgcaagtttt atcggcgaac gcaagatgct
      721 aatcatcttg gataactgtg aacatcttgt ctccgctact gctcacagta tcgaagccgt
      781 atccgccaaa tgccaggcag tgacctttct tgctaccagc cgtgagccgc ttggtgttgc
      841 aagtgaaatt ggctggcaaa caccagcatt ccctctacct gaacctggcg cgagtcatct
      901 caagactctt gcaaagaacg aatcggtgcg gctcttcgtc gagcgtggac gggccgtcaa
      961 tggcagcttt cagctcaacg gtcagaatgt gatgcgtgtg gcagagattt gtcgccgcct
     1021 cgaagggatg ccattagcga tcgagcttgc cgctgcacaa gtcaacaatc tcacgttaga
     1081 tggcatctta tcgcgcttgg aaaagtcgtt gaacttaacc tcaaccgatg ccgctcatgc
     1141 tcgtcacgct gcattgcatt ctgccattga atggagttac aacctgctta ccccagcaga
     1201 acaaatgctt tttcgtcggc tggcaatttt ttctggcgga tggaatctcg cctcagccgc
     1261 catcgtagcg gcaggctatt ctgaccccga agcgcctctt tcgaatgaag cttcagaagg
     1321 aacaatcgca ccattgccca tcaatggaga aacggttaca cgacagattc tggatgcact
     1381 tgtaaaaaaa gcattagcac aattccgtcg agcagaaggc agcttgcgat acgacacgtt
     1441 agacacctta cgagaatttg cacgcgaaaa aatgaatgaa gttggtgaaa cccagatcat
     1501 gcaagaacgg catgctctca cattttttaa ttttctaaaa aaagcttggg atgatgttta
     1561 ttcagaacgc gaatcaagca tattcaatca aatagatttg gaatatgcca atctgcgcac
     1621 cgcatgggca tggcaactgc aatcgccaaa atgggtggta gatgccaagg caattaatag
     1681 ccagaaccgt ttttggtatg tacgcggaca ttacgctgaa ggacgtgaaa ctctcgaagc
     1741 cttgctcaaa catcctgatg tacatttaaa tacactcatc catgccactt tgctgaacaa
     1801 ccttggttta atcgaatgga aaagcggaaa ccgtattgca gcaaaaagga ttttcgaaga
     1861 agcattagta gaatttcgaa ccctacaatt ggattttgcc gccgcaatga tcattgcgaa
     1921 cctggctggg ctttatatcg atgacgatga catggaaaag gggcttcccc tggcgcacga
     1981 agccgtggtc gtggcacgtg aaaccggaaa taaaagtgcc ctcaacatta gtttgaacaa
     2041 tcttgccatt gcgctcacca tcactcgtga tttcaaaggc gctcgcgaag ctttctatga
     2101 aggtcttgct ttgagcaaag aacaaggcga tatccgtggc gaaggtatta tctacaacgg
     2161 cttggcagat atcgaggcgg aagaaggcaa ttttgcagaa gcccgccgcc tgttcaccca
     2221 aagctttatg acgcgctcca gcctcggcga ccgccgtggt tccatcatcc ctgcactgaa
     2281 cttgatccgc ttaatgtgca tggatgaaga ttgggaaaat gccgcctgtt tgctagggtt
     2341 tgtggaagaa accctacaac aactcgggat tcaacttgca tctgatgcac gcgccgggcg
     2401 tgaagaagca aatcgttccg tccgcctcgc gctggatgag gaaactgtta ctcgtgccct
     2461 tcaatttggc aaaacactga cacttgattc ggcaaaccgc tggctggcag aaaaatccct
     2521 gtaacgctta tgtaacggta cgtttctatc atagaggaaa ttcaacaaag gagaatttct
     2581 catgaaacaa taccgtatgt tatttgcttt tgctgcgctg atgctt
//
LOCUS       DAOQNJ010000400         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_29750_length_2625_cov_8.517899, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000400 DAOQNJ010000000
VERSION     DAOQNJ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29750_length_2625_cov_8.517899"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1243)
                     /locus_tag="PLG52_10695"
     CDS             complement(218..1243)
                     /locus_tag="PLG52_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNB41958.1"
                     /translation="MKGTILYGARDVRYEERPDPQIIEPTDAIIKLSAACVCGSDLWA
                     YRGIDPVKQPVAMGHEYCGIVQEVGSEVKQVKPGQFVVGSFFASDNTCEICKSGYQSS
                     CLDRVGMGGIGTQAEYARIPLADGTLVATPELPSADLLPSLLSTSDVLGTGWFGALAA
                     EVAPGKTVAVVGDGAVGLLGILSAKQMGAERIIAMSRNPDRQKLALEFGATDIVTERG
                     DEGVAKIKDLTNGLGAHSVIEAVGTQESMMQSIRATRVGGHVGFVGVLHGVEIEGWEY
                     FFSHVHLHGGPAPVRQYLPHLIDLVLKGKINPGKVFDLTLPLSQVAEAYKAMDERRAI
                     KALLMPS"
     gene            complement(1261..2253)
                     /locus_tag="PLG52_10700"
     CDS             complement(1261..2253)
                     /locus_tag="PLG52_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB41959.1"
                     /translation="MNYRRLGKSGLKVSEISFGSWVTFSDQLNVDQALACMTAAYDAG
                     CNFFDNAEVYAAGQSEVVMGEALKKVGWSRDSYIVSSKVFFGAQSGDPKPTQRGLSRK
                     HVLEACHQALKRLQVDYLDLYFCHRPDRETPIEETVRAMTELILRGDILYWGTSEWTA
                     PEIMEAYSVAREYGLIPPTMEQPQYNLLHRQRVEQEYERLYEVIGLGTTIFSPLATGF
                     LTGRYNNGIPADGRANLPGYEWLKARLEGPTGEARLAAIRQWSKIADELGTSLSHLAL
                     AWCLKNPNVSTVIMGASRPEQVVENMKAVDVLPLLTDEVMKKIASVSVAGIEVD"
     gene            complement(2291..>2625)
                     /locus_tag="PLG52_10705"
     CDS             complement(2291..>2625)
                     /locus_tag="PLG52_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB41960.1"
                     /translation="YLTGKVDETTTFASNDIRARNPRFTQEAIKANRGVIELLERIAK
                     QKNGTPAQIALAWLLAQKEWIVPIPGSRKLERLDENNGAVNIELSADDLAQIDSAMSQ
                     INVIGNRY"
BASE COUNT          640 a          707 c          617 g          661 t
ORIGIN      
        1 ccatgaaaaa agcggcatac cgttgaactc ggcgatcatg cccggctggg caatgttgcg
       61 ggcattggca aaaagcaggc tgacaaattc ggcaataaaa atcgcccaca cgccgaaagc
      121 gactttttgg cgtgtggggt ttgtttgtac ttgagtcatg ttgattatga tgttccttca
      181 actatcatct attgaatgac agtctgctac attttattta tgacggcatc aacaacgcct
      241 tgatagcgcg gcgttcatcc attgccttgt aggcttcggc gacctgtgaa agcggaaggg
      301 tcaagtcgaa gactttgcca gggttgattt tgcccttcaa gaccaaatca attaagtgag
      361 gtagatactg gcgcacggga gcgggtccgc cgtgcagatg aacatgcgag aagaaatatt
      421 cccagccttc aatttccaca ccgtgcaata cgcctacaaa cccaacgtga ccacccaccc
      481 gcgtagcgcg gatggactgc atcatggatt cttgtgtgcc gaccgcttca atcactgagt
      541 gagcgccgag tccgttggtg aggtctttga tttttgccac gccttcgtcg ccgcgttcgg
      601 tgacaatatc tgtcgcaccg aattccagcg ccagtttttg gcgatctggg ttgcgactca
      661 tggcgatgat ccgctccgcg cccatttgct tggcagagag gattccgagc aagcccaccg
      721 ccccatcccc cacgaccgcc acggtcttgc cgggagccac ttccgcagcc agcgcaccaa
      781 accaacctgt acctaacaca tccgaagtgg agagcagact gggaagcaag tcagcagatg
      841 gcagttcggg tgtggcgacc aaagtcccat ccgcgagcgg aatacgggca tattccgctt
      901 gggttccaat gccgcccata cctacgcggt caagacagga agattgatag cccgatttgc
      961 aaatttcgca ggtgttatcc gaagcgaaga aagagccgac cacaaattga ccgggcttga
     1021 cttgttttac ttcactgccg acttcctgca caatgccgca atactcgtgt cccattgcga
     1081 caggttgttt tactgggtca atgccgcgat acgcccacaa gtcagaaccg caaacacatg
     1141 ccgcggagag ttttatgatc gcatcggtcg gttcgataat ttgcgggtca ggacgttctt
     1201 catagcgcac atcgcgtgcg ccgtataaaa ttgtgccctt catggtaaat ctccttattg
     1261 ctaatcaact tcaattccag ccacactgac actagcgatt tttttcatca cctcatctgt
     1321 caacaatggc aaaacatcca cagctttcat gttttcaacc acttgctcag gtcgagaagc
     1381 gcccataata accgtgctga cattaggatt cttaaggcac caagccaatg ccaaatgcga
     1441 aagacttgtt ccaagttcat cagcgatctt tgaccattga cgaatagccg ctaagcgcgc
     1501 ttcaccagtt ggtccttcca atcgtgcttt cagccactca taacctggta ggttggcgcg
     1561 cccatccgca gggataccat tgttatatct gccagtaaga aaacctgttg ccaagggaga
     1621 aaaaatcgtt gtacccaacc caatcacttc gtaaaggcgt tcgtattcct gctcaacacg
     1681 ctgacggtgc aacagattgt attgaggctg ttccatggtt ggagggatga gtccatactc
     1741 gcgggcaaca ctataggctt ccataatctc aggagccgtc cactctgagg taccccaata
     1801 gagaatatcg ccacgtagaa tcagttccgt catggcgcga acggtttctt caatgggcgt
     1861 ttcacgatca ggacgatgac aaaaatacag gtcaagataa tcaacctgaa gtctcttaag
     1921 cgcttgatgg caggcttcca aaacatgctt acggctcaac cccctttgag ttggtttggg
     1981 atctccagat tgagcaccaa aaaagacttt actcgagaca atataactat cacgtgacca
     2041 ccccaccttc ttgagcgctt cacccatcac tacttctgat tgaccagccg catagacttc
     2101 tgcattgtcg aagaagttac aacctgcatc ataggcagct gtcatacaag ccaaggcttg
     2161 atcgacattt aattggtcac tgaaggttac ccaagatcca aacgagattt cactgacttt
     2221 aagacccgat tttccaagac gccgatagtt catgttaaga ctcctttttt agcattcgtt
     2281 ttttctgttc ctaataccta ttaccgatca cattgatctg cgacatcgcg ctgtcaattt
     2341 gagcaaggtc atccgcgctt agttcgatat ttacggcgcc attgttttca tcgagccgtt
     2401 ccagtttgcg cgagccaggg atagggacga tccattcttt ttgggcgagc agccaagcca
     2461 gtgcaatttg tgcgggcgtg ccgttctttt gtttggcgat tcgttccagc aactcgatca
     2521 cgccgcgatt ggctttgatc gcttcctgcg tgaagcgcgg attgcgcgcg cggatgtcat
     2581 tgctcgcaaa ggtcgtggtc tcgtccactt tgcccgtcaa atatc
//
LOCUS       DAOQNJ010000401         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_30040_length_2610_cov_12.559295, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000401 DAOQNJ010000000
VERSION     DAOQNJ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30040_length_2610_cov_12.559295"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLG52_10710"
     CDS             complement(<1..644)
                     /locus_tag="PLG52_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB41961.1"
                     /translation="MTTLVKDLMHKGVITCKPDATLGQVATLLDQHQVHALFVTDRDG
                     RIVGVISDFDLMAGEWLSSDPESLNTMRKLTASDIMTKPVDTIQATTPLTEAADLFMQ
                     KRVGRFLVLENEKPVGTISLSDFVASLSQQIRSKRDTVGDVMSDAILVCRGKTPVLSA
                     ARAMTSSGWRSVLVVDVRGKVIGVVSGHDLLQLVKDGGAENKIVRDVMHPALTI"
     gene            772..1548
                     /locus_tag="PLG52_10715"
     CDS             772..1548
                     /locus_tag="PLG52_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNB41962.1"
                     /translation="MGKPLIPAQRREKIQEYLGIHQIARTADLMNLLEASEATVRRDL
                     EWLEQKGILERTHGGAILNQRVIFEQEYQLRLKNYPEEKKIIGELAASLIEEGDIVFV
                     NSGTTATQVLQHIPRNPRITVFTNNVSALVDIGDPGFSLNLTGGEFQARSNSLSGRFA
                     MDSLNMVFANKLILGVDGISLKHGLTVPTAPEAEVVRKMIERTKGQVIVVSDHSKWGA
                     VSNFHVANIDVMDTLVTDTGFSKQAKKELAEYSVNILVAG"
     gene            1622..>2610
                     /locus_tag="PLG52_10720"
     CDS             1622..>2610
                     /locus_tag="PLG52_10720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB41963.1"
                     /translation="MKTHAEIVVIGGGIYGAQVAYHLAKNGRKDVVIIEKGEIASGES
                     SHAAGLVTQFATSQAMLRFRMYSVQMYKELGLFDTVGSLRVASSKEQLLEMERSVSRA
                     KALGLDCEVISPEESKKYMPQISDKELYGGIYLPGDGQLDPYTVTTSMARFAKELGVE
                     IYTNTRVTGIKVSAKGEVEAVVTDKGEIRCEIIVNAAGMWAPRIAAMAGLHIPTTPVD
                     HQHIALRAVPGHEFNDQTPCLRDPDNLVYMRQEKGGLVIGGYEPKPLPRWIDGTPWEH
                     GSKSFPGDFDQFEMLLEGAIRRLPFLDQAGIITLVRHPGAYTPDCQPLLGPMPG"
BASE COUNT          643 a          588 c          723 g          656 t
ORIGIN      
        1 tcaatggtca atgctgggtg cattacatcg cggacgattt tattttcggc gccgccatcc
       61 ttcaccaatt gcagaaggtc gtgtccgctt accacaccga tcacctttcc tctcacatcg
      121 acgaccagaa ccgaacgcca gccggaggag gtcatggctc gcgctgctga aaggacgggt
      181 gtcttcccgc ggcacacgag gatcgcgtca gacatgacat cgccgacggt atcgcgcttt
      241 gatctgatct gttgtgaaag gctggcgaca aaatcagaaa gtgagatggt gccaacgggt
      301 ttttcatttt ccaaaactaa aaaccgccca actcttttct gcatgaaaag gtcggcggct
      361 tctgtcagcg gggtggtggc ttggatggtg tctacaggct tggtcatgat gtctgaggcg
      421 gttaacttcc gcatggtgtt gaggctttcg gggtctgaag aaagccattc gcccgccatc
      481 aggtcaaagt ctgaaatgac tccaacaatg cgaccatccc ggtctgtcac gaacaaggcg
      541 tgtacttgat gttgatcgag caaagttgca acttgtccca aagtagcatc gggtttgcag
      601 gtgatcacgc ctttatgcat aaggtctttg acaagtgttg tcatggaggc tccaaaaaag
      661 aagacccgac aggttttgtt tgattaagct gtcaggtcta gttaacgaat tcattataag
      721 caaaaaatca aaatcttgca aattccttca gattcttata taatccctca aatgggtaaa
      781 ccccttattc cagctcaacg cagagagaag attcaggagt atcttggcat tcatcagatt
      841 gcgcgtacag ccgatttgat gaatctgctt gaagcttctg aggcgacagt gcggcgtgat
      901 ctggaatggc tggaacaaaa gggaatcctt gaacgtactc atggtggggc aatcttaaat
      961 cagcgagtga tttttgagca ggaatatcag ctccgtttga agaactatcc tgaagagaaa
     1021 aaaattattg gtgaacttgc cgcctctctg atcgaggaag gcgatattgt gtttgtcaat
     1081 agcggtacta ctgcaaccca ggtcttgcaa catattcccc gaaatccgcg cattactgtc
     1141 ttcaccaata atgtaagtgc tttggtggat attggtgacc cgggcttttc tctcaatttg
     1201 actgggggtg agtttcaggc gcgttccaat tcgctctcag ggcgtttcgc gatggatagt
     1261 ttgaacatgg tctttgccaa taagttgatc cttggtgtgg acgggatatc cctaaagcat
     1321 ggcctgacag ttcccactgc gccagaagcg gaagtggtgc gtaagatgat agagcgtacc
     1381 aaggggcagg tcattgtagt ctctgatcat agcaagtggg gggcggtttc caacttccat
     1441 gtggcaaata ttgatgtaat ggacacattg gttactgata ctggttttag taagcaggca
     1501 aagaaggaac ttgcagagta ttcagtgaat attctggtgg ctggttgaga agtgcgggca
     1561 cgttcaggta acgtgtctgt ttgtttaata aattatcaaa tttcttcaac ggagttgaca
     1621 aatgaaaact cacgcagaga tcgttgtcat tggcggtggg atttatgggg cgcaggtggc
     1681 gtatcacctt gctaagaacg ggcgcaaaga tgtagtcatc attgaaaaag gggagattgc
     1741 cagcggtgag tcttcgcatg cggcggggtt ggtgacgcag ttcgccacct cacaggcgat
     1801 gttgcgcttc cgtatgtata gcgtgcagat gtataaagaa ctgggattat ttgatactgt
     1861 gggcagtttg cgtgtggctt cgagcaaaga gcagttactc gaaatggaac gcagcgtgag
     1921 ccgtgccaag gcgttgggac ttgactgcga agtcatctca cctgaagagt caaaaaaata
     1981 catgccgcaa atttcggata aggaattgta tggcggcatt tacctgcccg gtgacggtca
     2041 acttgacccg tacaccgtca ccacttcgat ggcgcgcttt gccaaagaac tcggcgtgga
     2101 aatttacacc aatacgcgcg tgaccggaat caaagtatca gcgaagggag aggttgaggc
     2161 ggtcgtaaca gataaaggcg agatccgatg cgagatcatt gtcaacgcgg cagggatgtg
     2221 ggcgccgcgt atcgccgcca tggcaggttt gcacatccca accactccag tcgatcatca
     2281 acacattgcg ttgcgagccg tgcccgggca tgagttcaac gatcaaacgc cgtgtttgcg
     2341 tgacccggat aatttggttt acatgcgcca ggagaaaggc ggcttggtga tcggtgggta
     2401 tgagccgaag ccgttgccgc gttggatcga cggcacgccg tgggagcatg gcagcaaatc
     2461 cttccccggc gactttgatc aatttgagat gctgcttgaa ggcgcgattc gacgtctgcc
     2521 atttttggat caggcgggca ttatcacttt ggtacgtcac cccggcgctt atacaccaga
     2581 ttgccaacct ctgctcggac cgatgcccgg
//
LOCUS       DAOQNJ010000402         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_30041_length_2610_cov_7.657534, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000402 DAOQNJ010000000
VERSION     DAOQNJ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30041_length_2610_cov_7.657534"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1288)
                     /locus_tag="PLG52_10725"
     CDS             complement(2..1288)
                     /locus_tag="PLG52_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNB41964.1"
                     /translation="MTSKHNFGFTTRQIHEGYTPDPATGSRAVPIYQTTAYDLQSTDR
                     AARLFALQENGNIYTRISNPTSNVFEKRVASLEGGIGALEVGSGHAAQATAILTICEA
                     GDHIVSTSLLYGGTVTQFKYTFPKLGITVTLVDPSDPENFRRAIQPNTKIIYGETISN
                     PLGNIFPFEEVSKIAHEHGIPLMIDNTFATPYLCRPFEWGADIVTHSTTKFIGGHGTT
                     MGGIIVDSGKFNWSKSGRFKNFTEPDPAYHHVIYADDFGPLAFISKARAGILRDLGTV
                     QQPIASWFLIQGLETLSLRMTRHVQNAKRAAEFLENHPNVKWVSYAGLPSHPDHERAQ
                     KYLPQGTGAVLGFGVKGGREAGAKFIDNLQLFSHVANIGDTRSLAIHPASTTHSQLSD
                     EEQTAAGVSPDFIRLSIGLEDIEDILWDLDQALSLI"
     gene            complement(1288..2214)
                     /gene="metA"
                     /locus_tag="PLG52_10730"
     CDS             complement(1288..2214)
                     /gene="metA"
                     /locus_tag="PLG52_10730"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNB41965.1"
                     /translation="MPVKIPSTLPAREILEYENIFVIDEERALHQDIRALRVAILNLM
                     PTKVATETQILRLLSNSPLQVEITLVHTATHESKNTDAEHLLQHYVTFEDIKHEKFDG
                     LIITGAPVEQLPFEEVDYWEELKQVMDWAKTNTESTFYICWGAQAGLYHYYGVPKYDL
                     PQKMFGVFEHRLLNRTESLMRGFDDIFLAPHSRHTEIRREDIEKVPELQILAESDDAG
                     VYIVGSRDGRELFITGHSEYDPLTLKAEYDRDVNKGLPIHVPKNYYPNDDPKQMPNVR
                     WRGHANLLYANWLNYYVYQATPFDPNEIPKRN"
     gene            complement(2288..>2610)
                     /locus_tag="PLG52_10735"
     CDS             complement(2288..>2610)
                     /locus_tag="PLG52_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNB41966.1"
                     /translation="PGLKSHPDYDRAKKYLPKGAGAILGFGIKGGAAAGKKFIESLQL
                     FSHLANVGDARSLAIHPATTTHSQLTEEQQVTAGVSPDFVRLSIGLEDFEDIKWDLDQ
                     ALNA"
BASE COUNT          587 a          561 c          746 g          716 t
ORIGIN      
        1 tctatattaa tgaaagcgcc tgatccagat cccagagaat atcttcgata tcttctagtc
       61 caatagagag gcgaatgaaa tccgggctga cgcctgcggc ggtttgctct tcgtcagaga
      121 gttggctgtg ggtggtgctg gcgggatgga tcgccagcga gcgcgtgtca ccaatgttgg
      181 cgacgtggct gaagagttgc aggttatcga tgaacttcgc tcctgcttca cgcccgcctt
      241 tgactccaaa cccgagtaca gcgcctgtgc cttggggtaa atatttctgg gcgcgttcat
      301 ggtctgggtg actgggtaaa cctgcgtatg aaacccattt cacattggga tgattctcaa
      361 gaaactcggc ggctcgtttc gcattttgca cgtgacgtgt cattcgcaac gataaggttt
      421 ctaacccttg aataagaaac caggatgcaa ttggctgttg gacggtgcct aagtctcgta
      481 agatgccagc gcgtgcttta gagatgaacg ccaatggacc aaagtcatca gcgtagataa
      541 catgatggta tgcaggatca ggctcggtga agtttttgaa gcgcccgctc ttactccaat
      601 tgaatttgcc actatctacg ataattccgc ccatggtggt gccgtgcccg ccgatgaatt
      661 tggtggtgga gtgggtgacg atatctgcac cccattcgaa gggacgacac aagtagggcg
      721 tggcgaaggt attatcgatc atgagcggga tgccatgttc gtgtgcgatc ttcgagactt
      781 cttcgaatgg aaagatattg ccaagcgggt tgctgatggt ttcgccgtag attatttttg
      841 tatttggctg aatggcgcga cgaaagtttt ccgggtcgct tgggtctacg agagtgacgg
      901 tgattccaag ttttgggaat gtgtatttga attgggtgac cgttccgcca tacaacaggg
      961 aggtggagac aatatggtca ccggcttcgc aaatagtgag gatggcagtg gcttgggcgg
     1021 catgaccaga gccaacttca agcgcaccga ttccgccctc gaggctggcg acgcgcttct
     1081 cgaagacgtt gctggttggg tttgagatgc gggtgtagat attaccgttt tcttgaaggg
     1141 caaagagtct tgcggcgcgg tctgtgcttt gcaggtcgta ggctgtggtt tggtagatcg
     1201 gaacagcccg actaccggta gcggggtctg gagtgtagcc ttcgtggatt tgcctagtgg
     1261 tgaagccgaa gttatgtttt gaagtcatta gttccgcttt ggaatttcat ttgggtcaaa
     1321 gggagttgct tggtatacgt aatagttcaa ccagttggcg tacagtaaat tcgcatgccc
     1381 gcgccagcgc acatttggca tttgcttggg gtcatcattt ggataataat ttttcggaac
     1441 gtgaataggt aaccccttgt tcacatcacg gtcgtattct gctttaagtg tgagagggtc
     1501 gtattcagag tgacccgtaa taaagagctc acgtccatct cttgaaccga cgatgtaaac
     1561 acctgcatca tctgattcgg cgaggatctg taattcaggc accttttcaa tatcttcgcg
     1621 gcgaatctca gtatggcggg agtgtggagc gaggaaaatg tcgtcaaagc cgcgcatcag
     1681 gctttcggtc cggtttaaga ggcggtgttc aaaaacgccg aacatttttt gcggcaggtc
     1741 atatttgggg acgccgtagt aatgatagag tccggcttgg gctccccaac aaatataaaa
     1801 cgtggactcg gtgttggtct ttgcccaatc catcacctgt ttgagttctt cccagtaatc
     1861 tacttcttca aaggggagtt gttccactgg cgcacccgtg ataatgagcc catcgaattt
     1921 ttcatgcttg atatcttcaa aggtgacata atgttgcagc agatgttctg catctgtgtt
     1981 cttggattcg tgggtagcgg tgtgaactag tgtgatctcc acttgcaacg gagagtttga
     2041 aagtaagcgc agaatctgtg tttctgtagc gaccttggtt ggcattagat tcagaattgc
     2101 cacacgcaag gcacggatgt cttgatgtaa ggcgcgctct tcgtcgatga cgaagatatt
     2161 ctcgtattcg agaatttcac gagcaggaag agtagaaggg atttttacag gcatgagttt
     2221 gaaatgatga tttatgcatg tggaatgaag aatttttttt tcttcattcc acatgtaatt
     2281 tctatgttta ggcgttaagc gcctgatcga gatcccactt gatgtcttcg aaatcttcga
     2341 gaccaatgct caagcggacg aaatcaggac tgacgccagc ggtgacctgt tgttcttcgg
     2401 tcaactgact gtgagtggtg gtagcgggat gaatggcgag agagcgggca tcaccaacgt
     2461 tggcaaggtg gctgaagagc tgcaggcttt caatgaactt cttgcccgcg gctgcaccgc
     2521 ccttgatacc gaacccgaga atcgcacctg cgcctttggg cagatacttc ttggcgcggt
     2581 cgtagtcggg gtggctcttg agcccaggat
//
LOCUS       DAOQNJ010000403         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_30076_length_2608_cov_10.047787, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000403 DAOQNJ010000000
VERSION     DAOQNJ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30076_length_2608_cov_10.047787"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..461
                     /locus_tag="PLG52_10740"
     CDS             180..461
                     /locus_tag="PLG52_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNB41967.1"
                     /translation="MTQSDLEEKRKKMVYSLLFVVIGQVGFITLIVLFALVWGGVWLD
                     NYFGTKPVITFVLSIAGIPLSVFLMFMASRRALARFTEQYEAEKKKISA"
     gene            472..1449
                     /gene="atpB"
                     /locus_tag="PLG52_10745"
     CDS             472..1449
                     /gene="atpB"
                     /locus_tag="PLG52_10745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNB41968.1"
                     /translation="METQKRTPWRRWIVLVLMVAGAYLASVFVPVRPEITVAAEKLIE
                     EPLIENFLGQPFYLVNTLPTLAVTIVLLVVIAFATNRSLVKSQKTDLIPSGIGNAMEA
                     ILEMLYNLTESSAGAKWAKAIFPWFATIMIYVLFANLLKLIPGFESIGVIHHAHGEGH
                     AVQHMGGFDMILPEKGEYILAPFFRGISVDLNFTLTLAVISFVMIQVIGVRAQGLAYF
                     SKFFNTKRMFKVPFFGAMDFLVGLLEVISEFAKILSFTFRLFGNMFAGVVLVAVVASL
                     LGNVSILAAMIMMFELFVGLIQAFVFGMLTMVFMAMATQGHGDEEHAEH"
     gene            1504..1746
                     /gene="atpE"
                     /locus_tag="PLG52_10750"
     CDS             1504..1746
                     /gene="atpE"
                     /locus_tag="PLG52_10750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNB41969.1"
                     /translation="MDGQFLLDAMRFVGAGLAMIGAAGAGVGIGLSVQGALGGMARNP
                     DTYGNLLTNMILGVAFSESIAIYCLVIAFLMLFKVV"
     gene            1764..2483
                     /gene="atpF"
                     /locus_tag="PLG52_10755"
     CDS             1764..2483
                     /gene="atpF"
                     /locus_tag="PLG52_10755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNB41970.1"
                     /translation="MEALGINVGLLIVQLIAFTIVMLTLNAWVYKPMLDMMETRKKKI
                     SQGLEDARVAAEARANAEKDAAKIVADAQAEASKVVREATERAEVASKDVRTAAEGEA
                     AKAREAAMAEAELERGRILGDLRGQVAALAIAAANKLVGEALDEKKQRALLDEFFSGV
                     KSGKVVVVGEELKGDTAVVTSALPLSNDEQAAVKKSVVAKDYSFKVDPSILGGLVIKV
                     DEKVLDASVAGKLEGLRSALK"
BASE COUNT          590 a          561 c          692 g          765 t
ORIGIN      
        1 aaggtgactg tcactttgta tcaaagtagg aaaagcctca gaaaatagag gcaagaatcg
       61 gctgggtaag aggcgttgtt cgagggcgcg gaaccgttct tcgggcggga aatccctcca
      121 aaagggattt ttcatttccg tttgacgctc ttagacctgt cagttataat tcgtgcgcta
      181 tgacacaatc ggaccttgag gaaaaacgca agaaaatggt atatagcctg ctttttgtag
      241 tgattgggca ggttggtttt attaccctaa tcgtgctatt tgccttggtt tggggcggtg
      301 tctggcttga taactatttt ggaacgaaac cggtgattac gttcgtattg tcgatagccg
      361 gtattccact ttcggttttt ctgatgttca tggcttcgcg cagagcgttg gcgcggttta
      421 cagaacagta tgaagccgaa aagaaaaaaa tttctgctta ggaggcgggg cttggaaacc
      481 caaaaacgta ccccctggcg gcgctggatt gttctggttt tgatggttgc gggcgcttat
      541 ctcgcaagcg tttttgtacc ggttcgacct gaaattacag tggctgccga aaaattgatc
      601 gaggaacctc tgattgagaa tttccttggg caaccgttct atcttgtgaa cacgcttccc
      661 actttggctg tgaccattgt cctgttagtg gtgattgcat ttgcgaccaa tcgctctctt
      721 gtgaagagcc agaagacaga tttgattcct tctgggattg gtaatgcaat ggaagccatc
      781 cttgagatgc tgtataacct gactgaaagc tcagcaggcg ccaaatgggc gaaggcgatc
      841 ttcccttggt ttgcgactat tatgatctat gtgttgttcg caaacttgct caagctgatc
      901 cctggttttg agtcgattgg tgtgattcac catgctcatg gcgaaggtca tgctgttcag
      961 catatgggtg ggttcgatat gattttgcct gaaaaaggcg aatacatact tgctcctttc
     1021 ttccgcggta tttctgtgga cttgaacttc accctcacat tggctgtgat ctcatttgtg
     1081 atgatccagg tgattggtgt ccgcgcgcaa ggtttggcat atttcagcaa gttcttcaat
     1141 accaagcgca tgttcaaggt gcctttcttc ggtgcaatgg actttttggt gggcttgctc
     1201 gaagtcatct ctgaatttgc gaagatcctc tcgtttacct tccgtctgtt tggtaacatg
     1261 ttcgcaggtg tggtgttggt ggcggtagtt gcctctttgt tgggtaatgt cagcattttg
     1321 gcagcaatga tcatgatgtt cgaattgttc gtaggcttga tccaggcgtt cgtgttcggt
     1381 atgttgacca tggtgttcat ggcaatggcg acacaaggcc atggcgacga agagcacgca
     1441 gaacattaat tttttgaaac ataattcata attaactagg ataaacctaa ggagactcga
     1501 aacatggatg gacaatttct tttagatgcg atgcgcttcg tgggcgcagg cttggcgatg
     1561 atcggcgccg ctggcgctgg tgtcggtatc ggtttgagcg tgcagggtgc tcttggtggt
     1621 atggctcgta accccgatac ctacggtaac ttgttgacca acatgatcct tggcgtggcg
     1681 ttctctgaat ccattgctat ttactgcttg gtcatcgcct tcctcatgct cttcaaagtc
     1741 gtgtaattca aggagcataa aacatggaag ctttaggtat taatgtcggt cttctcattg
     1801 ttcagctgat tgcattcact attgtgatgc tcacgttgaa cgcttgggta tacaagccca
     1861 tgctggatat gatggagacc cgcaagaaga agatctctca gggcttggaa gatgcccgtg
     1921 ttgcagccga agctcgcgct aacgcagaaa aagatgctgc caaaatcgtc gctgatgccc
     1981 aggctgaagc tagcaaggtt gttcgtgaag ctaccgaacg cgccgaagtt gcgtctaagg
     2041 acgtccgcac cgctgctgaa ggcgaagctg ccaaggctcg tgaagctgct atggctgaag
     2101 ctgaactcga acgcggtcgc attctcggtg accttcgtgg tcaggttgct gctcttgcta
     2161 ttgctgctgc caacaagctt gttggcgagg cacttgatga gaagaaacag cgcgctttgc
     2221 tcgacgaatt cttctctggc gtgaaatctg gcaaggtggt ggttgttggt gaagaactca
     2281 agggcgatac cgccgtggtc accagcgctc ttcctctttc caatgacgaa caggctgcag
     2341 tgaagaagag tgttgttgcc aaggattatt ccttcaaagt tgacccttcc atccttggtg
     2401 gtttggtcat caaggttgac gaaaaagtgc tcgatgcttc cgtcgccggt aaacttgaag
     2461 gtctgcgctc tgcactcaag taagactaaa acgatatcaa aaaggctttg gtaaaattac
     2521 caaagccttt tttgtttaac ttcaatggag ataaactagc aactatgttt ctcaaagact
     2581 tgttcgttga ctttcccttt tctcgtcc
//
LOCUS       DAOQNJ010000404         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_30236_length_2600_cov_10.822397, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000404 DAOQNJ010000000
VERSION     DAOQNJ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30236_length_2600_cov_10.822397"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..648
                     /locus_tag="PLG52_10760"
     CDS             157..648
                     /locus_tag="PLG52_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB41971.1"
                     /translation="MGDIFGLYSFVFIAVGFAFVAGVILLSNKPKWNDYLAFGVIVGG
                     LLVAWTILHPRQTLLMDDAKQVKDMIGAGTPVLLEFQSPYCIRCVSIKPIVDELEEEI
                     DPADIHIIRLNIQEQIGMELAPLYNFVATPTFIYFDSEGNEIWRMVGDFDPQRVRDSL
                     ANE"
     gene            641..1216
                     /locus_tag="PLG52_10765"
     CDS             641..1216
                     /locus_tag="PLG52_10765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB41972.1"
                     /translation="MNNLFRRLKFNYNYIAKKTPWDSGISPPELYDFMAAHSPGRALD
                     IGCGTGTNLITLANAGWQAEGFDFASRAVTIAKRKIQKAGVTVKVFTDDATQMKQVTP
                     PFDLALDLGCFHSITNKADYLTQLTRVLNPSGFWLVYGFIKPDPALTGPGITTADLDL
                     IQRHNLTLVSRKDGFDRRDRISAWFLWQANS"
     gene            1260..2174
                     /locus_tag="PLG52_10770"
     CDS             1260..2174
                     /locus_tag="PLG52_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41973.1"
                     /translation="MKILFLFLDGIGLGEDNPDTNPFLRANMPYLESLLGGKKMTKST
                     APFESDVLSLKAIDPNLGVKGLPQSATGQAVLVTGINIPAELGYHYGPKPNPEVAQYL
                     NGHTIFSKTVNAGKKATLLNAYPPRYFDGIDSGKRLYSSIPLALTNAGISLFTEKDYF
                     TGTALSADFTGEGWREFLGFPDAPLFDPETAGTKLAELASQYDFSFFEYWASDYAGHK
                     QDMPWAIKQLEVFDGVLKGLLTNWNMENDLVLLTSDHGNMEDLGTRKHTAAHVPLLLF
                     GAKTNRDAFTDVSDLTGVTPAMSSLLGL"
     gene            complement(2169..>2600)
                     /locus_tag="PLG52_10775"
     CDS             complement(2169..>2600)
                     /locus_tag="PLG52_10775"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNB41974.1"
                     /translation="LADREGTVRKLAAKMLGNLRDVRAIEELGMTLYDLHHEVSQTAA
                     QALAQFGAPAIDVLAEALIHPEASVREHAILGLGQIHDERVIPYLIEMLHDPAREVKR
                     QAIQSLAGIKDRRAIPALQELAGDRTDRELAAFAKEVIAKL"
BASE COUNT          685 a          700 c          553 g          662 t
ORIGIN      
        1 acgttgcgga taaactcggt ggtatactct ttcataagta agttaccttc ctgttcttat
       61 aagattgtga tgcaggttga gtgattgtaa aagaacttaa atgaattgtc aaagtgtgaa
      121 gcgtcacaca aaaaatatga cattgtagac tagataatgg gcgatatttt tgggctttat
      181 tcttttgttt ttatagcggt agggtttgca ttcgtcgccg gggtcatttt gctctcgaac
      241 aaacccaaat ggaacgatta tctagcattc ggcgtcatcg taggcgggtt gctcgtagca
      301 tggacgatcc tgcatccgcg tcaaaccctc ttaatggatg atgccaagca agtgaaagac
      361 atgatcggcg ccggcacacc cgtcctgctc gaattccagt ccccctactg tatacgctgt
      421 gtatccatca aacccattgt ggatgaactt gaagaagaaa tagacccagc cgatattcac
      481 atcattcgtt taaacatcca agaacagatc ggcatggaac tggcgccatt atataatttt
      541 gtcgccacgc ctacatttat atactttgac tcagaaggca acgaaatttg gcgcatggtc
      601 ggcgattttg acccgcagcg tgtgcgcgac tctcttgcaa atgaataacc tcttccgtcg
      661 attaaaattc aactacaact acattgctaa aaaaactccg tgggatagcg gcatctctcc
      721 accagagtta tacgacttca tggcagccca ctcccccggt cgcgccttgg atattggttg
      781 cggcacaggc accaacctca tcaccctggc aaacgctggt tggcaagccg aaggcttcga
      841 cttcgcctca cgtgccgtca caatagccaa aagaaaaatc caaaaagccg gtgtcacagt
      901 aaaggtcttt accgacgacg ccacacaaat gaaacaagtc acaccgcctt tcgatctagc
      961 cctcgacctc ggctgttttc acagtattac aaacaaagcc gattatctga ctcagttaac
     1021 tcgcgtgcta aacccaagcg gattttggct cgtctacgga ttcatcaaac cagacccggc
     1081 tcttactgga cccggaatta ccaccgctga cctagaccta atccaacgcc ataacctcac
     1141 actcgtctcc cgaaaagacg gcttcgaccg acgcgaccgt atctctgcct ggtttttgtg
     1201 gcaggcaaat tcgtaaggct ttttacgcat tacacatcat gtaatacgta aacctaccta
     1261 tgaaaatcct ctttctcttc cttgatggta tcggcttagg cgaagacaac ccagacacca
     1321 atcccttttt gcgcgctaat atgccttatc tcgaatcgtt acttggcggc aagaaaatga
     1381 ccaagtccac tgcgcctttc gagagcgacg ttctctcttt gaaagccatc gatccaaatc
     1441 tgggtgtaaa agggctgccc caatcagcca cagggcaagc agttcttgtc acaggcatca
     1501 acatccctgc tgaacttggc taccattacg gaccaaaacc caaccctgaa gtggcacaat
     1561 atctcaatgg tcacaccatc ttttcaaaga ctgtaaatgc cggcaaaaaa gcgacgcttc
     1621 tcaatgccta tcctccacgc tactttgatg gaattgattc aggcaaacgc ttgtattcat
     1681 ctattccatt ggcactaacc aatgcgggca tttccctctt cactgagaaa gattacttca
     1741 caggcaccgc cctctctgca gatttcaccg gcgaaggctg gcgagaattc ctcggttttc
     1801 ccgatgcgcc actctttgat ccagaaaccg ccggcacaaa acttgctgaa cttgccagcc
     1861 aatatgattt ttctttcttt gaatattggg caagtgacta cgcggggcac aagcaagata
     1921 tgccatgggc gatcaaacag cttgaagtat tcgatggcgt actcaaaggt ctgctcacga
     1981 attggaatat ggaaaacgat ctcgtcttgc tgacatctga ccatggcaac atggaagacc
     2041 tcggtacgcg caaacacacc gccgcacacg ttcctcttct cttatttggg gcaaaaacaa
     2101 atcgagacgc cttcacagac gtctcggact taaccggggt tacaccggca atgagttcat
     2161 tgttaggttt atagtttagc aatcacttct ttcgcaaaag cagctaactc tcggtccgtg
     2221 cgatctcctg caagctcttg caaggcaggg atggcacggc ggtctttaat gcctgccaaa
     2281 gattgaatgg cttgtcgctt cacttcacga gctgggtcgt gcagcatttc gataagatat
     2341 gggatgactc tctcatcatg gatttgtccc agcccaagaa tggcatgttc tcgaacagaa
     2401 gcttccgggt gaatcaacgc ctctgccaac acgtcgatag cgggtgcgcc aaattgagcc
     2461 aatgcctgcg ctgcggtttg actcacctca tgatgcaggt catacaaggt cattcccaat
     2521 tcttcaatcg cacgcacatc tcgtaaattg ccgagcattt ttgccgctaa tttacgaacc
     2581 gtcccttcac ggtctgccaa
//
LOCUS       DAOQNJ010000405         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_30300_length_2597_cov_8.684107, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000405 DAOQNJ010000000
VERSION     DAOQNJ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30300_length_2597_cov_8.684107"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1537
                     /locus_tag="PLG52_10780"
     CDS             47..1537
                     /locus_tag="PLG52_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB41975.1"
                     /translation="MKKITKIKNSIQTVTLVAVLFLSSCTSLPNNQTVASTSLPNSAV
                     EQSAVATAAFPTRRPYAPGELVDYVAQTGDTLPTLALRFNTSVDEILEANSFIPRDAT
                     TMPQGMPMKIPIYYRALWGSAFQIIPDHAFINGPTNIGFNTAAFLSTQTGWLSTYRAY
                     VGGEWKTGAALVDYAAINYSISPKLLLAILEYQGGALTQPAPPVNKNLLGLKRPFWDS
                     PYLQLVLAANTLNNGYYGWRTGTLLEFEDDDAILIRPDPWQNPGSVALQYYYSRMFTG
                     NDYNRAIGPDGLYKTYATFFGNPWENQFDLIPGSLSQPELSLPFPGDQTWSFTGGPHT
                     GWGTGFPFAALDFAPPAEQSGCVPAKPENFATAVADGLVVRSAQDGLALDLDRDGDER
                     TGWVIFYLHLATDQRATLGADLKTGDMIGYPSCEGGRSTGTHVHIARKYNGEWIPADS
                     ALPFVISGWVPHNGIEAYQGTLTKGGLTVTACVCGDAYTAISANYP"
     gene            complement(1551..2021)
                     /locus_tag="PLG52_10785"
     CDS             complement(1551..2021)
                     /locus_tag="PLG52_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB41976.1"
                     /translation="MDVTLNWQGKMAFEGAGPSGFVQRLDSDTSAGGDNSGARPMEMI
                     AIGLAGCTAMDVISILSKKQQKVADFQIKLHAERASDHPKVFTDSVIEYHVYGMDIDE
                     KSLVRAIELSAEKYCPAQAMLSKAFPIRLIYKIFDAETKSLLKEGEYQPKQKVA"
     gene            complement(2029..>2597)
                     /locus_tag="PLG52_10790"
     CDS             complement(2029..>2597)
                     /locus_tag="PLG52_10790"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB41977.1"
                     /translation="PANTTPLGRIMALVMIGFGFLALGIAFLLLRNQDEVSTGSSIDF
                     STVPAKVDFPAPDLSFIDLGTDPVSLSDYRGSVVLVNLWATWCPPCREEMPTLQAFYE
                     EYKSEGFVLIAIDQGETLPQVLPFVKELKLTFPIWLDYTSDAGRAFETMNLPSSYVID
                     RTGQVRLMWIGGISKKNLEKYVPDVIEE"
BASE COUNT          704 a          659 c          545 g          689 t
ORIGIN      
        1 gcagatacat tgagtcaggc ttggtcaaat ataatccgcg aaaaagatga agaagataac
       61 taaaataaaa aactccattc agacggttac tcttgtagct gtcttgttcc tttcctcttg
      121 tacatccttg ccaaacaatc aaacagttgc ttccacttca ctgccaaata gcgcagtgga
      181 gcaatctgct gtggcaactg ctgctttccc aactcgtcgt ccatacgccc cgggtgaatt
      241 agtagattat gtggctcaga ccggagatac cctaccgacc cttgctttac gcttcaatac
      301 cagcgtagat gaaattcttg aagcgaactc atttattccc cgtgatgcaa ccaccatgcc
      361 acaaggcatg ccaatgaaga tccctatcta ctatcgcgcg ctttggggta gtgcatttca
      421 gatcatccca gaccatgcct ttatcaatgg accaaccaat attggtttca acaccgcagc
      481 gtttctttca actcaaaccg gctggctttc cacctaccgc gcctatgtag gtggagaatg
      541 gaaaacaggc gccgctcttg tagattatgc ggcaattaac tatagcatta gccccaagtt
      601 attattggca atacttgaat atcaaggcgg tgcgcttaca caacctgccc cgcccgttaa
      661 caaaaacctg cttgggctga agcgtccctt ctgggattca ccttatttac aacttgtgct
      721 tgccgctaac acactcaaca acggatatta tggctggcgc acagggactc tgcttgaatt
      781 tgaagatgac gatgccattc tcatccgccc agacccatgg caaaacccag gttcggttgc
      841 gcttcaatac tattactcac gtatgttcac aggcaatgat tacaatcgag cgattggtcc
      901 agatgggctt tataaaacat atgccacttt ctttggcaac ccttgggaaa atcaattcga
      961 tctgattccc ggcagtttat cgcagccaga actttctttg ccattcccag gagatcaaac
     1021 ctggagcttc acaggtggac cccacactgg ctggggcaca ggttttccat tcgcagcgtt
     1081 ggacttcgcg cctcctgccg aacaatcggg ctgtgtacct gcaaaacctg aaaactttgc
     1141 caccgctgta gccgacgggt tggtcgttcg gtctgctcaa gatggcttgg ctctcgacct
     1201 cgaccgtgac ggtgatgaac gtacaggttg ggtaattttt tatttacatc ttgccaccga
     1261 tcaacgcgcc acgctcggcg cagacttaaa aacaggcgac atgattggtt acccgtcttg
     1321 tgaaggcggg cgttctactg ggacacacgt tcatattgcc agaaaataca acggcgaatg
     1381 gattcctgca gatagcgcat taccctttgt aattagtggc tgggtgccgc acaatggcat
     1441 agaagcctat cagggcaccc tcaccaaagg cggattaact gtcactgcct gtgtatgtgg
     1501 tgatgcttac accgccatca gtgcaaatta tccataaaac aaaaggtcgc ttacgcgacc
     1561 ttttgttttg gctgatattc gccttccttc aacagagatt ttgtctctgc atcaaatatt
     1621 ttatatatca atcgaatcgg aaaagctttg gaaagcatgg cttgtgcagg gcaatatttt
     1681 tctgccgaaa gttcaatagc acggacaagg gacttttcat caatatccat accataaaca
     1741 tggtactcaa tcaccgagtc tgtaaaaact ttaggatggt cactggctcg ctctgcatga
     1801 agtttgattt gaaaatctgc caccttttgt tgcttcttag aaagaattga gatcacatcc
     1861 atagctgtac acccagccag acctatagca atcatttcca tggggcgtgc gccactatta
     1921 tcgccgccag cagaagtatc tgaatctaag cgttgtacaa agccagaagg tcccgctcct
     1981 tcaaatgcca ttttgccctg ccaatttaat gtcacatcca ttttattttt actcctcaat
     2041 cacatcagga acatattttt ctagattctt ttttgaaatg cccccaatcc acatcaagcg
     2101 tacttgacca gtacggtcaa taacatagga gctgggcaaa ttcatagttt caaatgcacg
     2161 tccggcatca ctggtatagt ctaaccatat cgggaacgtt aatttgagtt ctttcacaaa
     2221 aggaagcact tgaggcaacg tctcaccttg atcgatagca atcaaaacaa aaccttcaga
     2281 tttatattct tcataaaatg cttgtagagt cggcatctct tcacggcaag gtgggcacca
     2341 tgttgcccac agatttacta atacaacact tccacggtaa tctgaaagag acacggggtc
     2401 agtgcccaag tcaataaaac tcaggtctgg ggcagggaaa tctacttttg caggcaccgt
     2461 tgaaaagtcg atggaagagc cggtggatac ttcatcctga ttcctcaaca gcaaaaaagc
     2521 gattcctagt gcgaggaatc caaacccgat catcaccaat gccataatac gcccaagagg
     2581 ggttgtgttt gcaggta
//
LOCUS       DAOQNJ010000406         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_30424_length_2592_cov_6.493496, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000406 DAOQNJ010000000
VERSION     DAOQNJ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30424_length_2592_cov_6.493496"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLG52_10795"
     CDS             <1..554
                     /locus_tag="PLG52_10795"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNB41978.1"
                     /translation="LIALTLPIAAVMAAGIHPLVRGVFGFDEQISTLITWTTRAYLAT
                     LTGYTIHEIAIRAFYARKEPMVPLYAIGIRLGIFLVIGILGVTFFQNIGAPVIAFAEI
                     ALLIEAIFILGWLSKRTHEPLHTSDVVFRGLIAAVAGGIVTYLVALYLPGGAIVTALI
                     GMIVGGVVALAIVWNEAKQLLKL"
     gene            607..1644
                     /locus_tag="PLG52_10800"
     CDS             607..1644
                     /locus_tag="PLG52_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41979.1"
                     /translation="MSEELESTQASRRSDLGGTQPSQPYKEDDTQPLKVKKPSRWRSI
                     LLGVLGFLLLISVGGFGGYSAGIGVRKNAEQSILATQLSEQFSFALVDIQFGRYENAR
                     QRLEYIIQRDPNFPGAQEKLTEVLVLSSVPTPTAAPTATATPDFSGAESAFARAQELT
                     RAQDWNGALTALDTVRKLDPSFKTAQVDGMYYFALRNRGYDLITKEGNLEGGIYFITL
                     AERFGPLDNAAKGVRDGARAYMTGASFWDLDWEQAAQYFETVAAGWPTMWDGTMTAGQ
                     RYYTAMLQLADEAFTAGEYCGAYEQYQKAASYGVLDGEASANSEQAYAACYPAEPAAT
                     EAPVVEPTATP"
     gene            1650..2156
                     /locus_tag="PLG52_10805"
     CDS             1650..2156
                     /locus_tag="PLG52_10805"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNB41980.1"
                     /translation="MDTPSTPVLAFIYWMHMLATVTWIGSLAAINLLFLPASTRTLKL
                     PDQLSLITALQKRLEPLAWFCMGILVVTGLFQMSTSPHYDGFLSISTQWSLSILIKHG
                     FAVIMVVVSAIQTWEVLPAIQRTLLKKDKADEAELSRLQKRETLLLRLNLLLSALILG
                     ATAFARVS"
     gene            complement(2207..>2592)
                     /locus_tag="PLG52_10810"
     CDS             complement(2207..>2592)
                     /locus_tag="PLG52_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNB41981.1"
                     /translation="DFVEQTRFDRAGAFQFSFEPGTTSAPLGDPVPAEVKQERYERLM
                     ELQQNISLQVNQSYVGKTLDVLVEGFDNGISVGRSYRDAPEVDGYVVVEGKAKVGDIV
                     PVRITGAFAYDLTGVLVEQQNLIKL"
BASE COUNT          655 a          722 c          566 g          649 t
ORIGIN      
        1 tccttatcgc cttaaccttg cccattgcgg ctgttatggc agcaggcatc catccgctcg
       61 tacgcggcgt cttcggcttc gacgaacaaa tctccaccct catcacgtgg acaacccgtg
      121 cctacctcgc cacattgacc gggtatacca tccacgaaat tgccatccgc gccttctatg
      181 ctcgcaaaga acccatggtt cctttatacg ctattggcat tcggctcggt attttcctcg
      241 tcataggcat tctaggggtg actttctttc aaaacatcgg cgctccggtc attgcctttg
      301 ccgaaattgc gctattaatt gaagcgatat tcatcttagg ctggctatcc aaacggactc
      361 acgaaccgct ccacaccagt gacgtggtct tcagagggct catcgccgca gttgcaggcg
      421 gaattgtgac ctacctcgtc gccctctacc tccccggcgg cgcaattgtc accgcattga
      481 tcggcatgat tgtcggtgga gtggttgcat tagcaattgt ttggaacgaa gccaagcaat
      541 tgttgaagtt ataaatgtca attaagaaaa aaaactgacc catcaaaaga atgtataatc
      601 acgcacatgt ctgaagaact cgaatccact caagctagtc gtcgctccga cctcggcgga
      661 acgcaaccct cacaaccata taaagaagat gatactcaac cgctcaaggt caaaaaacct
      721 tcacgttggc gttcaatcct tcttggtgtt ttaggcttct tactacttat tagtgtaggc
      781 ggcttcggcg ggtatagtgc ggggatcggt gttcgcaaaa atgcagaaca atccatcctt
      841 gctacccagc tttcagagca attctctttt gctctcgtag atattcaatt tggtcgctat
      901 gaaaatgccc gtcaacgctt ggaatacatc atccaacgtg acccgaattt ccctggtgca
      961 caagaaaaat taaccgaagt actggttctt agcagtgttc ccactcctac cgccgccccc
     1021 actgctacag ctacccctga tttcagcggt gcagaaagcg cttttgcccg agcacaggaa
     1081 ctcactcgtg cccaagattg gaacggagca ctcaccgcat tagacacagt tcgtaaatta
     1141 gacccttcct tcaaaactgc ccaagtcgat ggcatgtatt attttgcact gcgcaatcgc
     1201 gggtatgacc tcatcacaaa agaaggcaac cttgaaggtg gtatctactt catcacactc
     1261 gccgaacgtt ttggtccgct cgataatgct gccaaaggcg tacgcgatgg agcacgtgct
     1321 tatatgaccg gcgccagctt ctgggacctt gattgggaac aagccgctca atactttgaa
     1381 acagttgctg ctggctggcc cactatgtgg gacggcacaa tgactgcagg tcaacgctac
     1441 tatacggcca tgctgcaatt ggcagatgag gcgtttactg cgggtgaata ttgtggcgct
     1501 tatgaacaat atcaaaaagc agcatcctac ggcgttttag atggagaagc ctcagcaaac
     1561 tcagaacagg cgtatgcggc atgttacccc gccgaacctg cagcaacaga agctcccgtt
     1621 gttgaaccta cagctacgcc ataaacaata tggatacccc ctcaacacca gttctcgcgt
     1681 tcatctactg gatgcatatg ctggcaaccg tcacgtggat cggaagtctg gcagccatta
     1741 atttgctctt cctacccgcc tccacgcgga ccttgaaact gcccgaccaa cttagcctta
     1801 ttaccgccct gcaaaagcgg ctggaacctc ttgcatggtt ctgtatgggc atccttgtgg
     1861 tgaccggtct attccaaatg agcaccagcc cacattatga tggctttctt tcaatcagca
     1921 ctcaatggtc actatctatt ctcatcaagc atggatttgc cgtcatcatg gttgtcgtca
     1981 gtgccatcca aacctgggaa gtgctccccg ccattcagcg gactctacta aagaaagaca
     2041 aagcagatga agccgaactt tcccgtctac aaaaacgcga aacgcttttg ttacggctca
     2101 atttgttgct ctccgctctt attctcggag caaccgcatt cgcaagagta tcttgaatca
     2161 aaaaatcgga agtcgtaacg acttccgatt tttattttaa taacaattac agcttgatca
     2221 aattctgctg ttctaccagc acgccggtca gatcataggc aaaggcaccg gtgattcgta
     2281 cggggacaat atcccccacc tttgctttgc cttccacaac tacatatcca tctacttcgg
     2341 gtgcatcgcg gtaagagcgt cccacagaga tgccgttatc aaaaccttcc accaacacat
     2401 caagcgtttt gcctacataa gattgattga cttgcaacga aatattctgc tgcaattcca
     2461 tcaaccgctc ataacgctcc tgcttcactt cggcagggac tgggtcgcct aaaggcgcgg
     2521 aggtagtacc cggctcgaag gagaattgaa aagctccggc gcggtcgaag cgggtttgtt
     2581 ccacaaaatc at
//
LOCUS       DAOQNJ010000407         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_30516_length_2587_cov_9.609005, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000407 DAOQNJ010000000
VERSION     DAOQNJ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30516_length_2587_cov_9.609005"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLG52_10815"
     CDS             <1..542
                     /locus_tag="PLG52_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNB41982.1"
                     /translation="LAYSFIAGQLEHARALAGVVAPTVNSYKRLVPGYEAPVYIGWAQ
                     QNRSALIRIPRYTEGRDKAVRAELRFPDPSSNPYLAFTVMLAAALDGIDKNMKAPKPL
                     NNINLYHLNKEERAKLGVTELPGSLAESLKELEKNEVLKAALGDYLFEAYMRAKWEEW
                     DEFRLRVTDFEIQRYLETA"
     gene            639..1157
                     /locus_tag="PLG52_10820"
     CDS             639..1157
                     /locus_tag="PLG52_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41983.1"
                     /translation="MKKIFAFAILSVLLCSACSQNKAYPLKLATFDKNSVSVLIHLEK
                     DADENYFLSATFTPEQGFHLYSKDLPITGANGLGRPTLIEASVESQYQVTGKLLESVP
                     AEIPEFEPKDLLVYPVGAVTLRLSIELPPGNGWTDDSVKITYMACSDRGCKAPVIGQE
                     IAIRIPGKETFH"
     gene            1180..1746
                     /locus_tag="PLG52_10825"
     CDS             1180..1746
                     /locus_tag="PLG52_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB41984.1"
                     /translation="MKKYSLILIALLGVLACKALTPNPQEVETPVATDEPPSVIVQIT
                     DAPSEETAVTSSTGFTIVRLKPEDGDLATMLESESKKAQALGQMPVVEFDATWCPPCQ
                     AIDQSIGEENELMMNAYDGTYIIKLDVDVWGWDNGKVEMFQFEAIPVYFKLDTSGKPN
                     GEVIDGGAWGEDIPENIAPVMDAFFHNK"
     gene            complement(1794..2522)
                     /locus_tag="PLG52_10830"
     CDS             complement(1794..2522)
                     /locus_tag="PLG52_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB41985.1"
                     /translation="MSDIALEVRDLVKTFSLDGSTINAVDHVSFQVKFGEFVALVGPS
                     GSGKTTMLSILAALLTPTSGQVLIDGQDLSKMSEKERVKLRREKIGFTFQANNLIPYL
                     SARENVEFMLRLNGKLDHASRIRSDELLARLGLAERLHNLPAQMSGGQQQRVAIARAL
                     IHNPALVLADEPTASLDTERAFQVVETFANLIHENGRAGIIVTHDLRMCQYVDRVFQM
                     QDGKLIRTYETKNEIMELATATVH"
BASE COUNT          723 a          583 c          607 g          674 t
ORIGIN      
        1 cgttggcgta ttcgttcatc gccggtcaac tcgaacatgc ccgcgcgctt gctggcgtcg
       61 tcgctccgac cgtgaactca tacaagcgtt tagttccggg ttatgaagcg cctgtgtaca
      121 tcggttgggc tcagcagaac cgctcggcac tcatccgtat tccgcgctac acagaaggtc
      181 gcgataaagc cgtccgcgcg gaattgcgct tccctgatcc ttcgtccaac ccgtatttgg
      241 cgttcaccgt catgcttgcc gctgcgctcg atggtatcga caagaacatg aaggcgccta
      301 aaccactaaa caacatcaat ctctatcacc tgaacaaaga agaacgcgcc aagctcggtg
      361 tgaccgaact ccctggctcg ttggctgaat ctttgaagga attagagaag aacgaagtct
      421 tgaaggcagc tcttggagac tatctgtttg aagcctatat gcgtgccaag tgggaggaat
      481 gggatgaatt ccgcctacgc gtgacagatt ttgaaattca gcgctattta gaaacagcat
      541 aaacgttctg actagccaaa acaaaactgc actcagaaaa ttctgagtgc agttttgttt
      601 tattccttga aattcacttt gtcttataat tattttcaat gaaaaagatt tttgcttttg
      661 ctatcttaag tgttctatta tgcagtgcct gttctcaaaa taaagcatat ccgcttaaat
      721 tggcaacttt cgacaaaaat tcggtaagcg tcttaatcca cttggaaaaa gacgcggacg
      781 aaaattattt cctttctgct acgtttaccc cagagcaagg atttcatcta tatagtaaag
      841 acctacccat cacaggggca aatggacttg gacgtccaac attaatagaa gctagcgttg
      901 aaagtcagta tcaagtcacg gggaaattac ttgaaagtgt accagccgaa attccagaat
      961 ttgagccgaa ggatcttttg gtttatcctg ttggggcagt caccttgcgt ctgtccattg
     1021 agcttccacc cggaaatggt tggacagacg attcagtgaa aatcacctac atggcttgtt
     1081 cagaccgtgg ctgtaaagcg ccggttattg gtcaagagat tgcaattcgt atccccggca
     1141 aagagacgtt tcactaattc tataaaagat gggagactta tgaaaaaata tagtttgata
     1201 ttgattgctc ttctaggagt attggcatgt aaagcactaa cgccaaatcc acaagaagta
     1261 gagactccag tcgctacaga tgaaccgccg tctgtgattg ttcaaatcac agacgcacca
     1321 tcggaagaaa ctgctgtaac atcgtctact gggtttacca ttgtgcggct caagccagag
     1381 gatggagact tagcgacgat gctagaaagc gaaagtaaaa aagcacaagc gctgggtcag
     1441 atgcctgtcg ttgagtttga tgcgacctgg tgcccacctt gtcaggcaat tgaccagtct
     1501 attggtgaag agaacgaatt aatgatgaac gcctacgatg ggacctatat catcaagttg
     1561 gatgttgatg tctggggttg ggataatggc aaagtagaaa tgttccaatt tgaagctatc
     1621 cctgtatatt tcaagctgga tacttctgga aaaccaaacg gtgaagtgat tgatggcggt
     1681 gcatggggcg aggatattcc cgaaaacata gcacctgtca tggatgcctt ttttcacaac
     1741 aaataataaa aagggctcac ttttgaagtg agcccttttt atttataacc aatttagtga
     1801 actgtcgctg tagccagttc cataatttcg tttttagtct catacgtgcg gatcagttta
     1861 ccgtcttgca tctggaagac acggtctaca tattggcaca tacgcaagtc gtgcgtcacg
     1921 atgataccgg cacgtccatt ttcatggatg agatttgcaa aggtttcaac tacttggaag
     1981 gcacgctcag tatcgagtga agcggtgggt tcatcggcaa gtacaagtgc agggttatga
     2041 atcaacgccc gggcgatagc aacacgttgc tgctgaccac cagacatttg tgcaggcaag
     2101 ttgtgtaagc gttccgccaa cccaagccga gcaagtaatt catcagagcg gatgcggctg
     2161 gcatgatcca gcttgccgtt gaggcgcagc ataaattcca cattctcacg ggcagaaaga
     2221 tatggaatta aattattcgc ctgaaaggta aaaccgatct tctcgcggcg cagtttgaca
     2281 cgttctttct cgctcatctt tgaaaggtct tgcccatcaa tgaggacttg tccgctagtg
     2341 ggtgtaagta aggcagcaag gatggagagc atagtcgttt taccggaacc actaggaccg
     2401 accaacgcca caaactcacc aaactttacc tgaaacgata catgatctac tgcgttgatg
     2461 gtcgagccat cgagtgagaa ggttttgact aaatcgcgaa cttccaatgc aatatctgac
     2521 atatttaccc taacctaagc gccttgagcg gctcaatacg aacagcataa atgatagaga
     2581 ccattcc
//
LOCUS       DAOQNJ010000408         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_30572_length_2584_cov_10.381969, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000408 DAOQNJ010000000
VERSION     DAOQNJ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30572_length_2584_cov_10.381969"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..1310
                     /gene="corA"
                     /locus_tag="PLG52_10835"
     CDS             324..1310
                     /gene="corA"
                     /locus_tag="PLG52_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNB41986.1"
                     /translation="MIRSLYYKPGQPIRTNIPPTEFPRLIRDRKGTLWVDFIGEPEEL
                     AEPILRGFGFHPLAIDDALQETHTPKVDDWGDYIYLVLSILNYKQENGMFEAEIDELD
                     VFLGHNYVVTFHDQLLTAVEDTWIACQRDIRHVQDGADHLLYRIVDSLVMSYMPLVEQ
                     IDAQIDQIEDQVFDKPRRDILEQIFALKRLLLTMRRILLPQREVLNKLARDDFRVIDP
                     RDRIFFRDIYDHLVRLHDLNESLRDLVSGALDTYLSVTNNRMNEIMKVLTIITTFFMP
                     ITFITGFFGMNFFEPVAHLVGWTSQQVFAGMMSVMIGLPILMYFWMRGRTWL"
     gene            1430..2107
                     /gene="lexA"
                     /locus_tag="PLG52_10840"
     CDS             1430..2107
                     /gene="lexA"
                     /locus_tag="PLG52_10840"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNB41987.1"
                     /translation="MMMVRKSKGIGERHQKILDFIASYQREHKHPPSIREIGEHCDIS
                     STSVVNYYLDQLEKEGLLERDRKISRGMRLVNNSPLGDMLRVPVMGVIQAGEPIPIPA
                     SDFNPFTAEDTVDIATSLMPSKEKGKDLFALHVQGDSMIDAMINDGDIVVLKPAQEAR
                     NGEMVAVWLSDKNETTLKYFYKEKDGYRLQPANPTMKPIMIKKNEPLEIKGKVVMVIR
                     KMERLAA"
     gene            2216..2521
                     /locus_tag="PLG52_10845"
     CDS             2216..2521
                     /locus_tag="PLG52_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41988.1"
                     /translation="MYGTIARFRVRKDVNKEEFKQKMDEFGSAIIPGWIADYIFQTDA
                     SSNEYFLVAIFKDKATYQANADSAEQHERYLIFRSFLEDDPEWNDGTIVSATGPGSK"
BASE COUNT          714 a          633 c          570 g          667 t
ORIGIN      
        1 agcaaagcta ccttctattg aggtatcgca gctggagcag tgcaaacgaa caacttccat
       61 ttcagattgg cagatcgggc aacgggttgg ggcaggtcgc ataacaggct tctccgattt
      121 ataatgtgtc aaaaagtaat tttttagccg aaaatcgata tttttaagga cttttttcaa
      181 aaaatcaata aatttatcct gatttctaat aaaattatac aacagattta acttttgtca
      241 atagatttta cagaaaatca atatcaactt gaagattatt gagtttagct ttaaaatatt
      301 aaagtcgtcc ctggaggtca ctcttgattc gctctctcta ctacaaacct ggtcagccca
      361 ttcgcacaaa catcccccct actgagtttc cgcgcctcat acgcgaccgg aaaggcactc
      421 tttgggtaga tttcattggg gagcctgaag agctagcaga gcccattctg cgcggttttg
      481 gcttccatcc gctagccatt gacgatgccc ttcaagagac ccacaccccc aaagtggacg
      541 attggggcga ttacatctac cttgtgttga gcattttgaa ctacaagcaa gaaaatggaa
      601 tgtttgaggc agaaattgac gaattagatg tattccttgg gcataactac gtggtcactt
      661 ttcatgacca gttattgacc gcggtagaag atacttggat cgcctgtcaa cgagatattc
      721 gccacgtgca agacggagcc gaccatctgc tctatcgcat cgtcgactca cttgtgatga
      781 gctacatgcc gcttgtagag caaattgacg cccaaattga ccaaattgag gatcaagtct
      841 tcgacaagcc gcggcgtgac attcttgaac agatttttgc tctcaaacgc ctccttctaa
      901 cgatgcgccg gatcctgctc ccccagcgtg aagtgctgaa caagcttgcc cgcgacgact
      961 tccgcgtgat cgaccccaga gaccgtatct tcttccgcga tatatacgac catctcgtcc
     1021 gtttacacga ccttaacgag agcttacgtg accttgtcag cggcgcgttg gatacctatc
     1081 tttcggttac caacaaccgc atgaatgaga tcatgaaagt cctcacaatt attacaacct
     1141 ttttcatgcc cattaccttc atcaccggct ttttcggcat gaatttcttc gaacctgttg
     1201 cccaccttgt gggttggacc agccaacaag ttttcgccgg tatgatgtca gtcatgatcg
     1261 ggcttcccat cctgatgtat ttttggatgc gtggacggac ctggttatag ctccattttg
     1321 caccccaaaa tgccttgaca cagaacaggc gttcggttat cattattact agcacaaatg
     1381 agcgaagaat gctctgtacc tacgccagtc ccctggcagg aggcttgaaa tgatgatggt
     1441 tcgaaaaagt aaaggtatcg gcgaacgcca ccaaaaaatt ctggatttta ttgcgtctta
     1501 ccagcgcgaa cacaagcacc ccccttccat tcgtgagatt ggggagcatt gtgacatttc
     1561 ctccacttcg gtcgtgaatt actacttaga ccaattggag aaggaagggt tgctcgagcg
     1621 tgatcgcaag atctcgcgcg gcatgcggtt ggtaaataac agtccgcttg gcgatatgct
     1681 tcgcgtaccg gtgatgggcg tcattcaagc tggtgaaccc attccaatcc ccgcttcaga
     1741 cttcaacccc ttcactgcag aagacaccgt ggatatagcc acctctctga tgccttcgaa
     1801 ggaaaaaggc aaagacctct tcgcccttca cgtgcaagga gactccatga tcgacgccat
     1861 gatcaacgac ggcgatatcg tggtcctcaa accagcacag gaagcccgca acggcgaaat
     1921 ggttgccgtc tggctctcag ataagaacga aaccactctg aagtactttt acaaggaaaa
     1981 ggatggctat cgtcttcagc ctgctaaccc caccatgaag cctattatga tcaaaaagaa
     2041 tgaacccttg gagatcaaag gcaaagttgt catggtcatc cgcaaaatgg aacgcttggc
     2101 agcctaatca cttataaact atcaaaaaga agcggcatag atgccgcttc tttttgattc
     2161 ttgaggtgta ttaattattg aaagcgtctt atcgatacaa gaaaagaggt gacttatgta
     2221 cggaaccatc gctcgttttc gtgtcagaaa agatgtaaac aaggaggaat ttaagcaaaa
     2281 aatggacgag ttcggctcag ccatcatccc cggctggatc gcagactata tcttccaaac
     2341 cgatgccagc tcgaatgaat atttcctcgt agcaatcttt aaagataaag caacctatca
     2401 agcaaatgct gattctgcag aacagcatga acggtatttg atcttccgtt cgtttctgga
     2461 agacgacccc gaatggaatg atgggacgat tgtttcagca accggtccag ggtccaagta
     2521 aagaggcgca tgaatcaaaa aaggactgcc cgaaatcgga cagtcctttt ttgttccgag
     2581 tagt
//
LOCUS       DAOQNJ010000409         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_30749_length_2575_cov_9.825397, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000409 DAOQNJ010000000
VERSION     DAOQNJ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30749_length_2575_cov_9.825397"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..493)
                     /locus_tag="PLG52_10850"
     CDS             complement(50..493)
                     /locus_tag="PLG52_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNB41989.1"
                     /translation="MSNLIRWEPAREMMTLREAMDRLFDDAFTRPLSLSGNSWSIPAV
                     DMYQTDNEVVVKAALPGIKADEVQLNVTGEVLTIKGETKQENETKEKAYHIREQRWGA
                     FERSIVLPTEVVADKAKADFENGVLTITLPKAEEVKPKTISIKTK"
     gene            complement(722..1429)
                     /locus_tag="PLG52_10855"
     CDS             complement(722..1429)
                     /locus_tag="PLG52_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB41990.1"
                     /translation="MDRRRILVVDDEERMVRFIRMNLEHDGFQVGEAFNGKQAIQKIR
                     DFTPDLILLDVMMPDIDGFEVLETIRDFSNVPVIMLTAKGEEDDRVRGLEHGADDYIT
                     KPFSPRELVSRIKAVLRRTEGTGGSMHGLIEIDKRLKIDFERREIWLEGKLVKLRPTE
                     YRLLYHLVQNAGWVVSHDQLLQKVWGYEYRDEPHYVRLYINYLRQKLEKDPANPVYIL
                     TERGVGYRFVDFKRSKE"
     gene            complement(1443..1685)
                     /locus_tag="PLG52_10860"
     CDS             complement(1443..1685)
                     /locus_tag="PLG52_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41991.1"
                     /translation="MAYTVILHIANEASVAGEVDELPKPTDTIIVVANPRQKDGKDLH
                     YIDQGTVKVIWPLAKVSFVEILGDSEEEKIIGFVRE"
     gene            complement(1707..2180)
                     /locus_tag="PLG52_10865"
     CDS             complement(1707..2180)
                     /locus_tag="PLG52_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB41992.1"
                     /translation="MTGAMFCVECGAQLIGRDTLTTQNIATDSLGRASKRKTGELSQP
                     IEGVDAWANLQLLDTGQVLPLSSRNEFTLGRISEGQPIMPDIDLSPYQAYAAGVSRLH
                     AVIKRSGSYVKFMDLGSANGTFLNGIRLSPNVEQSIQHGDIVSLGKMRIQILIEK"
     gene            complement(2210..>2575)
                     /locus_tag="PLG52_10870"
     CDS             complement(2210..>2575)
                     /locus_tag="PLG52_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41993.1"
                     /translation="IASNPIPASPIRLKLTRPLEQSPSAEPPPTRVLKALSRLTLYRM
                     QERAQSAAQNGQFDVAARHLQNLATHLLSQGEHAVAKTALLEAQSLENTRTWSESGSK
                     DIKYKTRALLLDSGKEKKS"
BASE COUNT          644 a          648 c          571 g          712 t
ORIGIN      
        1 acagacgaag agattcttcg cttcgctcag aatgacatta caaaaagttt tacttggtct
       61 tgatgctgat ggtcttgggt ttgacttctt ccgctttggg gagcgtgatg gtcagcacac
      121 cgttctcaaa gtccgctttg gctttgtcgg caacgacttc ggttggcagg acgatcgagc
      181 gttcgaaggc gccccagcgt tgttcgcgaa tgtgatacgc cttctctttg gtttcgttct
      241 cctgcttggt ctcaccttta atagtaagta cctcaccggt cacattgagt tgaacttcat
      301 cggctttgat gccgggcaaa gcagccttca ccacaacttc gttatctgtt tggtacatat
      361 ccacagccgg tatagaccac gaatttccgg aaaggctgag cgggcgggta aacgcatcgt
      421 cgaagaggcg atccatggct tcacgcaggg tcatcatctc gcgagcgggt tcccaacgaa
      481 ttaagtttga catagctacc tccttgcagg atttgaattt tgtcttacgt ccattaactt
      541 aaaacgcctg cgttagaaaa acgcaagcgt tttgttttcg ttttataaga gaagcagttc
      601 aggggctgaa ggtggtgaaa agcacaacca atccgtaaat cacaccaatg caggtcacac
      661 aaacgccaac cagctttgcc gccgtcaccc acttattctc aggtttttgc tccgtcatgg
      721 gttactcctt actgcgtttg aaatcgacaa agcgatagcc aactccacgc tcagtgagaa
      781 tgtaaaccgg attagccgga tccttctcca acttttggcg cagatagtta atatacaaac
      841 ggacgtagtg cggttcatcg cggtattcat agccccaaac tttctggagc aattggtcgt
      901 gagaaaccac ccaacctgca ttctgcacaa gatgatacag caatcgatat tctgtggggc
      961 gaagcttgac gagcttgcct tcgagccaaa tctcacggcg ttcaaaatcg atcttcaagc
     1021 gtttatcgat ctcgatcagt ccatgcatgg agccgcctgt gccttcggtg cggcgcagta
     1081 cggctttaat acggctgacc aattcacgcg ggctgaacgg tttggtaatg taatcgtccg
     1141 cgccatgttc gagtccacgc acacggtcat cctcttcacc tttggcggtg agcataatga
     1201 ccggcacatt actaaaatcg cggatggtct caagcacttc gaagccgtca atatccggca
     1261 tcatcacatc aagcaaaata aggtcgggag tgaaatcacg aatcttttgg attgcttgct
     1321 tgccgttaaa cgcctcccct acctgaaaac catcatgctc caggttcata cgaataaagc
     1381 gcaccatacg ctcttcatca tccacaacca ggatgcgacg tcgatccatt tctgtcatac
     1441 gtctattccc tcacaaaacc aatgatcttc tcttcttcag agtcaccaag gatttcgaca
     1501 aaacttactt tcgctaaagg ccaaatcacc ttaacggttc cttgatcgat atagtgcaag
     1561 tctttgccat ctttctggcg gggattggca acaacaataa tcgtatcggt tggtttagga
     1621 agctcatcca cttcaccagc cacagatgct tcattagcaa tatgcaaaat aacggtgtaa
     1681 gccatatccg ttgtctctcc ttcgttctat ttctcaatta atatttgaat tctcatctta
     1741 cccaatgaca caatatcacc gtgctgaatc gattgctcca catttggtga taaacgaatg
     1801 ccatttagaa aggtgccgtt tgcagaaccc aagtccataa atttgacata agagccgctt
     1861 cttttgatga cagcatgtaa gcgtgaaacc ccagcagcat atgcctggta aggagagaga
     1921 tcaatatccg gcataattgg ttgcccctca ctaattctcc ctaacgtgaa ttcatttcga
     1981 gaagagagag gtaacacttg cccagtatct aaaagttgta gattcgccca agcatcaaca
     2041 ccttctattg gctggctcaa ttcaccggtt tttcgtttgc ttgctcttcc aaggctatct
     2101 gtcgcaatat tctgggtggt tagtgtgtct cttccgatca actgggcgcc acattccaca
     2161 cagaacatgg cacctgtcat atttgcatgt ttgcaattgg aacagattat catgattttt
     2221 tctccttgcc actgtccaat aacagcgctc gtgttttata ttttatatcc ttgctcccag
     2281 attcgctcca ggtacgcgta ttttcaagac tttgtgcttc aagcaatgca gtttttgcaa
     2341 cagcatgttc gccttgtgaa agcaaatgcg ttgctaaatt ttgcaaatgc ctggctgcta
     2401 catcaaactg tccattctgg gcagcagatt gcgcccgttc ttgcatgcga tacagagtaa
     2461 ggcgggaaag cgccttcaaa acccgcgtcg gcggtggctc tgctgaggga ctttgctcca
     2521 agggacgggt cagtttgagg cggatgggag atgcagggat aggattggaa gcaat
//
LOCUS       DAOQNJ010000410         2573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_30796_length_2573_cov_11.428515, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000410 DAOQNJ010000000
VERSION     DAOQNJ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30796_length_2573_cov_11.428515"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..883
                     /locus_tag="PLG52_10875"
     CDS             11..883
                     /locus_tag="PLG52_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNB41994.1"
                     /translation="MKIHLIDGTYELFRAHFGAPPKKAANGQEVGATLGMLRSMLLLL
                     SDPEVTHVAVAFDHVIESFRNKMYAGYKSSEGVDPVILNQFPLAEAAIKALGLVTWPQ
                     VKFEADDAIATAVAKFKKVKSVEQIVICSVDKDLTQMVEGDRVICWDRRREIFLNEKG
                     VTEKFGVPPESIPDFLALVGDSADGYPGIQGWGEKSTATVLAKYKHIEAIPDDPNKIP
                     LSLGRATTLLGNLKNNYKDALLFRELSTLRTDVPLKESLADLKWLGVYPRFKKVCDEL
                     GNQSIPERITKWRK"
     gene            899..1471
                     /locus_tag="PLG52_10880"
     CDS             899..1471
                     /locus_tag="PLG52_10880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB41995.1"
                     /translation="MLYLIVISILIVMVTLSALWMIVPAFFGPPSIPTARSRIRKALQ
                     LANLQPGEMVYDLGAGDGRVLLMAAQEFNAQAVGVEIGPVQCLIIQLQVLASGYGDKV
                     RVRWDNYFKTNLKDADVVFIYATSRETGKLANHLKQQMKPGSRLVSISADFPDWEPSH
                     IDDLELIFLYTMPPKDGSMMTYLMKNSANS"
     gene            1492..>2573
                     /locus_tag="PLG52_10885"
     CDS             1492..>2573
                     /locus_tag="PLG52_10885"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNB41996.1"
                     /translation="MLTIDIEVARRFILGKQGVWPGRRWRGLKGTEQAMRSMEYLQLD
                     PLQIIARSQDISLHSRVINYKPELWEDAAYKQRKFFDWGGWLAARPMDELPYWRVVMH
                     RERDGGYGDSRIRQMAKEHAKAIVEVRAILKDRGTVGNRDFEAADRKKTQSYRGRKDS
                     ALALYYLWRIGDVMTHHRERFERVYALTENVAPKELIYEKTQEEADSFLVKKEIRFGG
                     ITRIQRTGDSYGRGEPDRAAKKLLEGMLADGEIIEVKVDGLKAIQYALADDEKIISGL
                     SAGRIPKAWKPVDTTTTEEAVFLAPLDHVSARGRAKELFGFEYVWEVYKPEHQRKFGY
                     YVLPVLWGDRLVARFDSKLDRTTNTF"
BASE COUNT          642 a          542 c          723 g          666 t
ORIGIN      
        1 aggagactgc atgaagatcc atttgattga cggaacgtat gaactcttcc gcgctcattt
       61 tggcgcgccg cccaagaaag ctgcgaacgg acaggaagtt ggcgccaccc ttggaatgtt
      121 gcgtagtatg cttttgcttc tctctgatcc cgaagtgact cacgtcgctg tggcgtttga
      181 ccatgtgatc gagtcgtttc gcaacaagat gtacgcaggc tataaatcca gcgaaggcgt
      241 ggaccctgtg attctgaatc agtttccatt ggctgaagcg gcgatcaaag ccttgggatt
      301 ggtgacctgg ccccaggtca agtttgaggc ggatgatgca attgctacgg ctgtggcgaa
      361 gttcaagaag gtcaaatctg tggagcagat cgtcatttgt tccgtcgata aagatttgac
      421 ccaaatggtg gaaggtgacc gcgtcatttg ttgggatcgt cgtcgtgaaa ttttcttgaa
      481 tgaaaaaggt gtgaccgaaa aatttggtgt cccacctgaa tcgattccgg atttcctcgc
      541 cttggtgggg gattcggctg atggctaccc aggtattcaa ggctggggtg agaaatctac
      601 ggcgacagtg ttggcgaagt ataagcatat tgaggctatt cccgatgacc cgaacaaaat
      661 tccacttagc ttggggcgag ccacaacgtt gcttgggaat ttgaaaaata attacaagga
      721 cgctttgctt ttccgcgaac tttccacctt gcggacggat gtgccgttga aagaatctct
      781 agcagatttg aagtggctgg gcgtttatcc acgctttaag aaggtctgtg atgaattggg
      841 gaatcaaagc attccggaac gtatcaccaa gtggagaaag taaacacgtt ttactgatat
      901 gctttatttg attgtcatct ctattctaat tgtcatggtc accctcagcg ccttgtggat
      961 gatcgttccg gcatttttcg ggccgccttc cattcccact gcgcggagtc gcattcgcaa
     1021 ggcattgcaa cttgcaaacc ttcaacccgg ggagatggtg tatgaccttg gcgctggtga
     1081 tggccgtgtg ctgttaatgg cagcccagga atttaatgca caagctgttg gtgttgaaat
     1141 tggcccggtg caatgtctaa ttattcaatt gcaggttctt gccagcggtt atggagataa
     1201 ggttcgagtg cggtgggata actatttcaa gacaaatttg aaagatgcag atgtggtgtt
     1261 tatttatgcc acttcaagag aaacgggcaa actggcgaat catttgaaac aacaaatgaa
     1321 gcccggttca cggcttgttt ccatttccgc cgatttcccc gattgggagc cttcccatat
     1381 cgatgacttg gaattgatct tcctttacac catgccgccc aaggatggaa gcatgatgac
     1441 gtatttgatg aagaactcgg ctaattctta atttgaaaag gcttcggtac aatgttaacg
     1501 atagacattg aggttgcccg gcgttttatc cttggaaagc agggggtgtg gcctgggcgt
     1561 cgctggcgtg gactgaaagg cactgagcaa gccatgcggt caatggaata tttgcagctt
     1621 gacccgttgc agatcatcgc acgcagtcag gatatctctt tacatagccg tgtcatcaat
     1681 tacaaacccg agttatggga agatgctgct tataagcaaa gaaaattctt cgattggggt
     1741 ggctggttgg cggcacgccc catggacgag ttgccctatt ggcgggtggt gatgcatcgc
     1801 gaacgcgatg gcggctatgg agattcgcgc attcggcaaa tggcaaagga acatgccaag
     1861 gcgatcgttg aagtccgcgc tattttgaag gatcgtggaa cggtggggaa tcgtgatttt
     1921 gaagcggcag accgtaaaaa gactcaaagt taccgcggac gaaaagacag cgcactggca
     1981 ttgtattatt tgtggcgcat cggcgatgta atgacgcatc atcgcgaacg ctttgagcgg
     2041 gtctatgcgc tgacggagaa tgttgccccg aaagaattga tctacgaaaa gacacaagaa
     2101 gaggcggaca gttttctggt taagaaagag atccgctttg gcggcatcac acgcattcaa
     2161 cgtaccggcg attcgtatgg acgcggcgaa cccgaccggg cggcgaaaaa acttctcgaa
     2221 ggcatgcttg cagatggcga gatcatcgaa gtaaaagtgg atggactgaa ggctatccaa
     2281 tatgcgttgg cagatgatga gaagattatt tcaggtttaa gcgcgggtcg gattccaaaa
     2341 gcgtggaagc ctgttgatac aacgaccact gaagaagctg tttttcttgc gccactggat
     2401 catgtcagcg cacgcgggcg ggccaaggaa ttatttgggt ttgagtatgt ttgggaagtg
     2461 tataagcccg aacatcaacg caagtttggt tattatgtgc ttcccgttct atggggagac
     2521 cggctggttg ctcgttttga cagcaagttg gatagaacga ccaatacatt tat
//
LOCUS       DAOQNJ010000411         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_31116_length_2559_cov_7.271965, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000411 DAOQNJ010000000
VERSION     DAOQNJ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31116_length_2559_cov_7.271965"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLG52_10890"
     CDS             <1..527
                     /locus_tag="PLG52_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNB41997.1"
                     /translation="NDTNSHVTVSSWRRVDDNAIPARSKVAGAYVNSALAKTDANRAG
                     FDEALVLTSEGHISEGSAMNVFMVRNGVLHTPPLTDNILEGVTRRSVIEVAQKELGLE
                     VVERSIDRTEILIADEVFLTGSAAQVVAVTKIDYRPIGSGVMGPITAKLRDIYEDIVR
                     ARNSKYANWNVIVK"
     gene            complement(565..1302)
                     /locus_tag="PLG52_10895"
     CDS             complement(565..1302)
                     /locus_tag="PLG52_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB41998.1"
                     /translation="MSKYKYDYYDDAPEESKAPMRLDIWDMLSILGILATLCIGAYFA
                     AVFVSPNASYNLLNPVRNQPPTPTITQIQPPATWTPTPLGPTETATLTLVPTFTLEPS
                     PTVVSLITPSVTPTATRTPKAPFFATVTYIDSTIIHPEAACNWQGVAGTIIDANNADM
                     LGIAIRLTGFYNGKSKNELTVSGIAPAYGKSGFEFFLSAVPVASDNLLTIQILDQAGL
                     PLSDNIPINTYADCTKNMVLVKFKKNP"
     gene            complement(1325..2203)
                     /locus_tag="PLG52_10900"
     CDS             complement(1325..2203)
                     /locus_tag="PLG52_10900"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNB41999.1"
                     /translation="MQFKRIFLTLFLAVGISACGSLGTSSTPTPSEPTPTLAPPTPTP
                     PPSAAAVNGEYITITEFQAELERYKAAQTALGLSFTDEDANKVVLEDMIAQVLLSQAA
                     RAENFEVTDADLQSRIDALVSQLGSADALIQWQSAHGYDDASFRLALKRSIEAAWMRD
                     KIVAAVPTSAEQIHLRQILTYNEADAQSALAELNAGIDFDELAAQYDPVTAGELGWVP
                     QGYLLDPKADEAVFALQTGQVSGIISTDAGFHIFKAIERGDHALSPDALLAMQEHALQ
                     NWVADQRAKSEIVIAP"
     gene            complement(2279..>2559)
                     /locus_tag="PLG52_10905"
     CDS             complement(2279..>2559)
                     /locus_tag="PLG52_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNB42000.1"
                     /translation="NAGQDGSVIIDTVRRTAAEKKNPNIGYNVLTSEYTDMIKAGVID
                     PVKVVRGALENAASIAAMILTTDVLITDMPAKENAPAMPPGGMGGMDY"
BASE COUNT          680 a          608 c          651 g          620 t
ORIGIN      
        1 aaaatgacac caactcgcat gtcacagttt cttcctggcg gcgcgtggac gataacgcca
       61 tccctgcacg aagtaaagta gctggcgcat atgtcaactc tgcgttggca aagaccgatg
      121 caaaccgcgc cggctttgat gaagctttgg tattgacgtc agaaggtcat atctctgagg
      181 gatctgccat gaatgtattc atggtccgca acggcgtact gcacactcct ccgctcaccg
      241 ataacatcct tgaaggcgtt acccgtcgtt ctgtgatcga agttgcccaa aaggaattag
      301 gtcttgaagt tgtagagcgt tccattgacc gtacagagat cctgatcgcc gacgaggtct
      361 ttttaaccgg cagcgcagcc caggtcgttg ctgtaacaaa gatcgactat cgccccattg
      421 gcagcggtgt gatgggaccc atcactgcca aactgcgtga tatctatgaa gatatagtac
      481 gcgcccgaaa ttcaaaatac gcaaattgga atgtgattgt caaataaaat aaaaaatccg
      541 ccgagtgctc ggcggatttt ttatttatgg gttcttctta aatttcacca ataccatatt
      601 cttcgtacaa tctgcatagg tattgatcgg aatattatca gaaagcggca accctgcctg
      661 gtcaagaatt tgaatcgtga gtaagttatc agacgcaact ggaaccgcgc tcaagaaaaa
      721 ctcaaaaccc gattttccat aggctggagc aataccgctc acagtaagtt cattcttgct
      781 tttaccgtta tagaagcctg tcaaacgaat agcaatgcca agcatatctg cattattcgc
      841 atcaataata gtaccagcca caccttgcca gttacaagca gcttccgggt ggataatggt
      901 gctatcaatg tacgtcactg tagcaaagaa aggagccttg ggtgtacgtg ttgcggttgg
      961 agtaacagac ggggtaatca gggaaaccac tgtgggagaa ggttcaaggg taaatgtagg
     1021 caccaatgtt aatgtggcag tttccgtcgg tccaagcgga gtgggcgtcc atgtggcagg
     1081 cggttgaatt tgtgtaatcg taggagtagg aggttgatta cgaactgggt tcaacaaatt
     1141 gtatgaggca tttggtgaga caaacacagc cgcaaaataa gcaccaatac aaagagtagc
     1201 caaaataccc agaatgctca acatatccca aatatcgagc cgcatgggcg cttttgactc
     1261 ctcaggggca tcatcataat aatcgtattt gtatttgctc ataaatcact ccaaaaaatc
     1321 acacttaagg tgcgatgaca atttcacttt tcgctcgttg gtcggcgacc caattttgta
     1381 aagcatgttc ttgcatggca agcaaagcat caggcgaaag ggcatggtcg ccgcgctcaa
     1441 tcgctttgaa gatatggaaa cccgcatccg tagaaataat gccacttact tgacccgttt
     1501 gtaaagcaaa aacggcttca tccgctttgg ggtctagcaa atatccttgt ggcacccaac
     1561 ctaattcgcc agcagtcacc gggtcatatt gagccgccaa ctcatcaaaa tctatgccag
     1621 cattaagttc agccaatgct gattgcgcgt ccgcctcatt ataggtcaaa atctggcgca
     1681 aatgaatttg ctcggcagat gtaggcacag ccgcaacgat tttgtcacgc atccatgccg
     1741 cttcaataga acgctttagt gccaagcgga aggatgcatc atcataccca tgcgcagatt
     1801 gccattgaat aagcgcatcg gcactgccaa gttgacttac caacgcatca atacgtgact
     1861 ggaggtctgc atcggtcact tcgaagtttt ctgcccgcgc agcctggctc aacaatactt
     1921 gcgcgatcat gtcctcaagg acaaccttgt tcgcatcctc atcagtgaag gaaagtccca
     1981 gcgcagtttg ggcggctttg tagcgttcca gttccgcttg gaattcggtg atggtgatgt
     2041 attctccatt gacagccgct gcagaaggcg gaggagtagg agtaggcggt gcgagagtgg
     2101 gagtcggctc agaaggggtg ggcgtactcg aggtcccgag agaaccgcag gcagatatcc
     2161 ccacagcgag gaacaaagtc aaaaatatcc gtttgaattg cattcgtaaa attataaatt
     2221 aaaaacgtag gggcgacccg ttgggtcgcc cctacaaatt tgtggggaaa ggattggttt
     2281 agtaatccat gccgcccatg ccgccaggag gcatggcagg agcgttttcc ttcgcaggca
     2341 tgtcggtgat gagcacgtct gtggtgagga tcatcgcggc gatggaagcg gcgttttcga
     2401 gcgcaccgcg cacaaccttg accgggtcaa tgacgccagc cttgatcatg tcagtgtatt
     2461 cgctggtgag aacgttgtaa ccgatgttcg ggttcttctt ctcagcagcg gtgcggcgga
     2521 cggtgtcgat gatgacggag ccatcctgac cagcgttgg
//
LOCUS       DAOQNJ010000412         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_31243_length_2554_cov_8.744298, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000412 DAOQNJ010000000
VERSION     DAOQNJ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31243_length_2554_cov_8.744298"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PLG52_10910"
     CDS             <1..1215
                     /locus_tag="PLG52_10910"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNB42001.1"
                     /translation="TLEMSGFGDSRKLAAQFYFTELTVLKVMFTAIVTAMVLLFGAVG
                     LGFLDFNQVWVNPTYLSSGILGGLIMGVGFIIGGFCPTTSLASASTGKIDGMFFMLGG
                     FVGAFIFGETEKYFDAWYNYSGYYGRLTLDQVFNLPIGVVVLLVILMALFMFWGAEQL
                     ERLIGKKDMSKEPKIRLAGAGALLVAAIAVVAIGAPSLADRYNKLTFTRTETVEQLNA
                     DPIINTYVYTADQMLEKRLVFITPAEAYKAKYNQAMNPIYLDVRSEADYNLYHIEDAV
                     NVPLERITEIVPMLLSEPPTNSVFIVMSNDEAAAIQAWKLLSASSVSNVYILEGGVNN
                     WIAFFGKDDTTFTAQPNPDAGDDQLAYIFPSALGSRYESCSPNPVEYEALEFEAKIKL
                     QLKRDKSGGGCG"
     gene            1340..1933
                     /locus_tag="PLG52_10915"
     CDS             1340..1933
                     /locus_tag="PLG52_10915"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNB42002.1"
                     /translation="MNISRLLEKSMKDHDHLCPRQILGVRIGLAGMKALGFSEPPQHK
                     QMLVISEIDGCFVDGVIAATDCTVGHRTLRVEDYGKVAVTFVHVKTGRAVRIAPALDI
                     REQATLFVPSEPRHYFAQMQAYQTMPDETMLNITKVELTTPIQEIVSRPGVRVNCDVC
                     GEEIMNEREVHSNGHVFCQSCYSHESYYHSVIRQKFP"
     gene            complement(2001..>2554)
                     /locus_tag="PLG52_10920"
     CDS             complement(2001..>2554)
                     /locus_tag="PLG52_10920"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB42003.1"
                     /translation="MNILPAVADDVLKGDSQTFGLLMGASGAGALISTLFLLPLSQQA
                     KRIGRIVGGAAVWMGTFYLLLSRSTYLPLSMLSIFLVSLGAPLVLTTGLGVMQLMAPG
                     DMRARIISLFTMVSFGLQPLSSLIIGYAAETFGTQTIIMINAACLTLGGILMLMLRKG
                     LIQWELKPMVEPILSSQEKAAMH"
BASE COUNT          661 a          599 c          613 g          681 t
ORIGIN      
        1 acccttgaaa tgtctggttt tggtgactct cgtaaacttg cggctcagtt ctacttcacc
       61 gagttgaccg tgctcaaagt gatgttcacc gccattgtca cagccatggt cttgctgttt
      121 ggtgccgttg ggcttggctt tttggatttc aatcaagtat gggtcaaccc tacgtatctt
      181 tcctccggca ttcttggcgg tttgatcatg ggtgttggct tcatcattgg cggcttctgc
      241 cccactacct cgctcgcatc cgcatccact ggcaagatcg atggcatgtt cttcatgctc
      301 ggtggttttg ttggcgcatt tattttcggt gaaaccgaaa aatacttcga tgcttggtac
      361 aactactctg gctattacgg tcgtctcaca ctagatcagg ttttcaacct gccaataggt
      421 gtggttgtat tgctcgtaat tttgatggcg ctcttcatgt tctggggtgc agagcagctc
      481 gaacgactga tcggcaaaaa agatatgagt aaagagccga agatccgcct tgctggggct
      541 ggtgcactcc tcgtagcagc tattgctgtt gttgccattg gtgctccgtc attagcagac
      601 cgctacaata agttaacctt cactcgcact gaaacagtcg agcaattgaa tgcagacccg
      661 attatcaata cctatgtcta caccgccgac caaatgcttg aaaagcgttt ggtgttcatt
      721 acgcctgctg aagcctataa agcaaaatac aatcaggcga tgaacccgat ctatttggac
      781 gtgcgttccg aagcagatta caacttgtat cacatcgaag atgcggtcaa cgtgccacta
      841 gagcgtatca cagagattgt acctatgttg ctctctgagc cacccacaaa ttcagtcttc
      901 atcgtcatga gcaatgacga agccgctgcc attcaagcct ggaagcttct ctctgcatcc
      961 agtgtatcga atgtctacat tctggaaggc ggagtcaaca actggatcgc tttctttggc
     1021 aaggatgaca cgactttcac ggctcaaccc aaccccgatg ctggagatga ccagttagcc
     1081 tatatcttcc cctctgcctt gggcagccgc tatgagtctt gctcgccaaa tccagtggaa
     1141 tatgaagccc tagaatttga agccaagatt aagttgcaat tgaaacgcga taaatctggc
     1201 ggaggttgtg ggtaagatca tcgcaagtaa atattacacc cctgaagcat ttacttcagg
     1261 gatgtttttt tgatgctatg cgcacatttg gaatatattc ggcagttata attattctta
     1321 gatcgcccga aagactcgta tgaacatttc acgtttgctt gaaaaatcta tgaaagacca
     1381 cgatcacctt tgcccaaggc aaattttagg tgtgcgtatt gggttggctg gcatgaaggc
     1441 gctcgggttt tcagaaccac cgcaacataa acagatgttg gtcatttctg aaatagatgg
     1501 gtgttttgtc gatggtgtga ttgccgcgac agattgcacc gtaggtcacc gcacattgcg
     1561 tgtggaagat tacggcaaag ttgccgtcac gtttgtgcat gtaaaaacgg gtcgcgctgt
     1621 gcgcattgcg cctgcattgg acatccgcga acaagccact ttattcgtgc caagcgaacc
     1681 acgtcattac tttgctcaaa tgcaggcgta tcagactatg cctgatgaaa ccatgttgaa
     1741 catcacaaag gtggaattga ccacccccat tcaagaaatc gtctcgcgtc ccggagtacg
     1801 ggtcaactgt gatgtatgcg gtgaagagat catgaatgag cgtgaagtcc atagcaacgg
     1861 tcatgtattt tgccaaagtt gctattctca tgaaagctat taccattctg ttattcggca
     1921 gaaatttccg tgaaaatatc cagctatagg tttatagaat cgaaaaggac tctcgtttga
     1981 gagtcctttt cgattaaggc ttagtgcatc gctgcttttt cttgagaaga taagatcggc
     2041 tctaccatgg gtttcagttc ccattggata agtccctttc tcagcatcag catcaggatt
     2101 ccgccaaggg ttaagcatgc agcattgatc ataatgatgg tttgtgtacc aaaggtttca
     2161 gcagcatacc caataatcag agaggataac ggttgtaaac caaaagacac catcgtgaag
     2221 agactaataa tgcgggctcg catatcgccg ggcgccatga gttgcatcac acccaatccc
     2281 gtcgtcagca ccaatggggc tccaagacta acgaggaaaa tagacaacat agaaagtggc
     2341 aaatatgtgg aacgggaaag taaaaggtag aaagtgccca tccacaccgc agcaccgccc
     2401 acaatgcgac cgatccgttt ggcttgctgg gagagtggca ataagaacaa ggtagagatc
     2461 aaagcgcccg cgcctgatgc acccattaag agaccaaatg tttgcgagtc gcctttgagg
     2521 acgtcatcgg ctacggcggg gagaatgttc atga
//
LOCUS       DAOQNJ010000413         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_31393_length_2547_cov_7.934591, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000413 DAOQNJ010000000
VERSION     DAOQNJ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31393_length_2547_cov_7.934591"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLG52_10925"
     CDS             <1..446
                     /locus_tag="PLG52_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42004.1"
                     /translation="SEPAVISEVLQLDVSQTGEVAVTVVVPELTQSSVPTATPEAVEE
                     ADAFVTQEGYPRVSAWSLAMLFVLLGSGALYVIASRFTKRMIATRWSLGMLLGGLFVY
                     NLMAFGFFDLPSWLLEAGISGVLYATLTGQLLGFAAGWAWSRLEK"
     gene            527..1552
                     /locus_tag="PLG52_10930"
     CDS             527..1552
                     /locus_tag="PLG52_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNB42005.1"
                     /translation="MESNGKAGKNKVIKAKQPPILFDKTQAIIADLTKKLGGTLVTYF
                     NNPRGAVCHDDVLALFELLEQIGKQEKIYLFLKSSGGNGQASLRIVNLLRQYCEELIA
                     VIPLECASAATMITLGANEIHMGPMAYLTSVDTSLTHALSPIDRDNDRVSVSLDELNR
                     VVKLWQAQRSDSNENPYQQLFQHVHPLVIGAVDRAESLSIMLCKELLAYHIKDEQVGE
                     KIASTLNSKYPSHGYPILYEEAHRIGLKVSRLSPEINSQLLELNELYSEMGQRATTDF
                     DDTHAHGNEILNIWESTDVLVYYQQDKDWFYRTEERRWISLNDNSSWRRMHKKGNKVE
                     KSILHIS"
     gene            1678..2277
                     /locus_tag="PLG52_10935"
     CDS             1678..2277
                     /locus_tag="PLG52_10935"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HNB42006.1"
                     /translation="MATNIHLKFNDQAPDLTLLDGAGNPVQLSSLWKKQVLVLAFTRH
                     FGCPQCKEMVDQLIDSHQTLTEAGLLLAIVSHASPESAKAFCDQRAPNALCLADPDRT
                     VYKAYGLYRGTLWQTILSPNIWKSNRRLARQKGIKPGVPSPGQDVYQMSGTFVIGMDG
                     KIRLPYYYEDIADHPPVDLLLHGIMGVDWKKPFDSNPLV"
     gene            2291..>2547
                     /locus_tag="PLG52_10940"
     CDS             2291..>2547
                     /locus_tag="PLG52_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42007.1"
                     /translation="MKTFFLATYLALKEVFRNRGRFLLVALVIALITMLVLFIAALGE
                     GLGNGNRQYIANLDADLIVFLEKSDYIISASRLEVNTARSIR"
BASE COUNT          637 a          578 c          616 g          716 t
ORIGIN      
        1 ccagcgaacc tgctgtgatc tctgaagtat tacagttgga tgtctcacag actggtgaag
       61 tcgcggtgac ggtggtcgtg ccagagttga cacaatcttc tgtgccaact gccacgcctg
      121 aagcggtaga agaggcggat gcctttgtca cgcaggaagg ctatccgcgg gtttcggctt
      181 ggtctcttgc catgctcttt gttttgctgg ggtctggcgc tttgtacgtg atcgcttctc
      241 gttttaccaa acgaatgatt gctacacgtt ggtcgttagg aatgttgtta ggtggtttgt
      301 tcgtatataa cctgatggca tttggctttt ttgaccttcc aagttggttg ttagaggcgg
      361 gaatatctgg cgtgctgtat gccacgctta caggtcagtt attaggtttt gctgctggct
      421 gggcttggtc acgcctggag aaatagtact atcaacttat tttgtttcta gcgttcagtg
      481 ttaccattta ttggattgat atcattaagg ttaggagacg aattatatgg aatctaacgg
      541 caaagctggc aaaaataaag tgatcaaagc aaagcaacct ccgattctgt ttgacaagac
      601 tcaggcaatc attgcagact tgaccaaaaa actcggcggc acgcttgtca cgtatttcaa
      661 caatccgcgc ggtgcggttt gccatgacga tgtgttggct ttatttgaac tactcgaaca
      721 gatcggcaaa caggaaaaga tttatttatt cctaaaatcc agcggcggta atggacaagc
      781 ttctttgcgt attgtgaatc ttttacgaca gtactgtgaa gaactaattg ctgtgattcc
      841 tctggaatgt gcttcagcgg cgaccatgat tacattgggc gcgaacgaaa tccatatggg
      901 acccatggca tatctcactt cagtggatac ttctttgacg cacgcattat ctcccattga
      961 ccgcgacaac gaccgcgtga gcgtcagctt ggatgaattg aatcgtgtcg tgaaactgtg
     1021 gcaagctcaa aggtccgact cgaacgagaa cccttatcaa caactctttc agcatgtgca
     1081 tccgcttgtg attggcgccg tggaccgtgc tgagtctctt tccattatgt tgtgcaaaga
     1141 attacttgcc tatcacatta aagatgagca agtgggtgag aagatcgcat cgacacttaa
     1201 ctctaaatat ccttcgcatg ggtatcccat tttgtatgaa gaagcacacc gtattggctt
     1261 gaaggtaagc cgcctttcac cagaaatcaa ttcgcaattg cttgagctca acgagcttta
     1321 cagcgaaatg ggtcaacgcg ctacaacaga ttttgacgac actcatgcac atgggaatga
     1381 gattctcaat atttgggaat cgaccgatgt cttggtgtat taccagcaag acaaagattg
     1441 gttctatcgc actgaagagc gccgctggat ttcgctcaac gataatagca gttggcggcg
     1501 aatgcacaaa aagggtaaca aggtcgagaa atcgatcctg catatctcgt aagaatttga
     1561 ggcacagtag ggaactgtgc ctcttttttt ggttggggga gggtaaaaac aactcgttgc
     1621 aaaacctgta caaaacctgt gcattgtgct aaaatctcgt cattcaaaaa ggtaactatg
     1681 gcaacaaata ttcacctcaa gtttaatgac caagctcccg acctaaccct cttggacggc
     1741 gcaggcaacc ctgtgcagct ttcatctctc tggaagaagc aagtccttgt gctggcgttt
     1801 acccgtcact ttggatgccc gcaatgcaaa gagatggtgg atcaattgat cgactcgcat
     1861 cagactctga ccgaggctgg gttgctgctc gcaatcgttt ctcacgcctc acccgaatca
     1921 gccaaagcgt tttgtgacca gcgtgctccg aatgcgttgt gcctcgctga cccagaccgt
     1981 accgtttaca aagcctatgg tctttaccgt ggcactctct ggcaaaccat cctttccccc
     2041 aatatttgga agtccaatag aaggttggcg cgacagaaag gcatcaaacc tggtgtacca
     2101 tcccccggac aggatgttta tcaaatgtct ggcacctttg ttattggtat ggatgggaaa
     2161 attcgtttgc cttattacta cgaagatatt gccgatcatc cacctgtaga tttgctttta
     2221 catggcatca tgggtgtgga ttggaagaaa ccgttcgact caaacccttt ggtctaggaa
     2281 gtacgaaata atgaaaactt tttttcttgc aacttacctt gcgctcaagg aagtgttccg
     2341 caatcgcgga cgttttttgc ttgtggcgct cgtcatcgcg ttgatcacga tgttagtcct
     2401 cttcattgct gcattagggg aggggttggg caatggtaac cgccaatata ttgccaactt
     2461 agatgcagac ctgatcgtct ttttggagaa atcagattac attatctctg ccagccgctt
     2521 ggaagtgaac accgctcgtt ccattcg
//
LOCUS       DAOQNJ010000414         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_31585_length_2538_cov_6.455497, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000414 DAOQNJ010000000
VERSION     DAOQNJ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31585_length_2538_cov_6.455497"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="PLG52_10945"
     CDS             <1..1669
                     /locus_tag="PLG52_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNB42008.1"
                     /translation="MLEIGEYLERPDHNFARGQSAQFNLEPEYKGTMRRCENCHDASK
                     GHADWLPYIETHMDAVACETCHIPQMYAPAIQTFDWTVVKTNGSAVETCRGIEGAPND
                     VTSLITGYEPALLNRTNIDGQQLLAPYNLITSFYWVYDDANGNTRPVRQIDLEAAYLE
                     NGKYNTDIVAAFDDNGDGQLTEDELTINSSAKEEAVKTKLTALGLNNVRMEGMVQPYS
                     INHNVTKGEYAVNDCKACHDADSRISQSIKLADSAPVIPVFDTDNNVNSTGEIVKGKD
                     GALYYQPVPANDKMYVFGSSRVNWVDWFGALAFAGSLLGVLGHGTLRYLAGRKQPKGH
                     ERTERVYMYDAYRRFWHWLQTFAIVILLFTGLIIHRPDVFGAFSFRGMVTIHNILAVI
                     LVINALLSVFYHIATDRMQEYIPRPYGFFDDAMVQAKYYISGIFKGEPHPFEKTPTRR
                     MNPIQHATYFGILNVLLPLQMVTGALMWGVQKFPTFANALGGLPFLAPFHSLVAWLFA
                     TFILVHVYMSTTGATPLEATRAMITGYEEVEVHEVHEEPKNEKKGKK"
     gene            1745..2290
                     /locus_tag="PLG52_10950"
     CDS             1745..2290
                     /locus_tag="PLG52_10950"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNB42009.1"
                     /translation="MTTQTKKSVFNRPEKPYVHPYFGGLVLGIILFLAFFLTGNGLGS
                     SGATSRIDAAIVDAISPSHVDNTPYLLKMAGGDKNPFDEWVVPVFFGALIGGFASGFF
                     NGRLKFMTTKGPNISDRTRWLMAFLGGVIFLYGARMARGCTSGQALSGGATLSAGSWV
                     VMFAIFGGAYGLAYFVRKLWN"
     gene            2301..>2538
                     /locus_tag="PLG52_10955"
     CDS             2301..>2538
                     /locus_tag="PLG52_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42010.1"
                     /translation="MNFPLSVLIVPSAEHPWTYLVFLVIGIAFGYTLEMSGFGDSRKL
                     AAQFYFTELTVLKVMFTAIVTAMVLLFGAVGLGFL"
BASE COUNT          615 a          665 c          598 g          660 t
ORIGIN      
        1 catgttggaa attggcgaat atctcgaacg cccagaccat aacttcgccc gcgggcaaag
       61 cgctcaattc aaccttgagc ctgaatacaa aggcaccatg cgccgttgtg aaaactgcca
      121 tgacgccagc aagggtcatg ccgactggct gccctatatc gaaacccata tggatgccgt
      181 cgcttgcgaa acctgtcaca tcccgcagat gtatgctcct gccattcaaa ctttcgattg
      241 gaccgtggta aagaccaatg gttctgccgt cgaaacttgc cgtgggattg aaggtgcccc
      301 caatgacgta acgtctctta tcacaggtta cgaacctgca ttactcaacc gcaccaatat
      361 tgatgggcaa caattgcttg ccccatataa cctcatcact tctttctact gggtctatga
      421 tgatgccaat ggcaacaccc gcccagttcg ccaaatcgac ctcgaagcgg catatctcga
      481 aaatggcaaa tacaacacag atatcgtagc tgcatttgac gacaatggcg acggtcaatt
      541 gaccgaagac gagctcacaa ttaactcttc tgccaaagaa gaagctgtca aaacaaagct
      601 tactgcgctt gggctgaaca acgtccgcat ggaaggaatg gtgcagcctt acagcatcaa
      661 ccacaatgta accaaaggcg aatatgccgt caacgactgc aaagcatgtc acgacgcaga
      721 ctcgcgcatt tcacaatcca tcaaacttgc agattctgct cctgtgatac ctgtattcga
      781 cactgataac aatgtgaaca gcactggcga gattgtcaaa ggaaaagatg gggctttgta
      841 ctatcaacct gtacccgcca atgacaagat gtatgtcttt gggtccagcc gcgtaaattg
      901 ggtggactgg ttcggagcat tggcattcgc tgggtcgctt ttaggtgtgc ttgggcatgg
      961 aacgctgaga tacttggcag ggcgcaaaca gcccaagggt cacgaaagaa ccgagcgtgt
     1021 gtacatgtat gacgcatatc gccgcttctg gcattggctc cagaccttcg cgatcgtcat
     1081 cctcctcttc actggtttga taattcatcg accggatgtc ttcggtgcct tctccttccg
     1141 cggcatggtg accattcaca acattttggc tgtgatcttg gtcatcaatg ccttgctctc
     1201 ggttttctat cacattgcaa cagaccgtat gcaagaatac attccgcgcc catacggctt
     1261 cttcgacgat gccatggtgc aagcgaaata ttacattagc ggcattttca aaggtgaacc
     1321 acatccattt gaaaagacac ccacacgcag aatgaacccc attcaacatg caacctactt
     1381 cggcattctc aatgttttgt tgccgctcca aatggtaact ggtgccttga tgtggggagt
     1441 gcaaaaattc ccgacgtttg caaacgcact tggtgggttg cctttcttgg caccgttcca
     1501 ttctctagtt gcatggttgt tcgctacctt catcctagtg catgtgtaca tgagcaccac
     1561 tggcgctact ccgctcgaag cgacccgcgc catgatcact ggctatgaag aagtggaagt
     1621 acatgaagtg cacgaagaac cgaaaaacga gaagaaaggc aaaaagtaaa gaataacgcg
     1681 ggctgtgtat atcatgatgt acacagcccg gatcaggttc atgctttacc tataggagtt
     1741 ttcaatgaca actcaaacta aaaaatctgt tttcaaccga cctgaaaagc cctatgttca
     1801 cccttatttt ggtggattgg ttttagggat catcctcttc cttgctttct ttctcactgg
     1861 caacggactt ggttcttcag gtgccacctc acgcatcgat gcggcaatcg tagatgccat
     1921 ctcgccatcg catgtagata acacgcccta tttgttgaag atggctggcg gcgacaagaa
     1981 tccgttcgat gaatgggttg taccggtctt cttcggcgca ttgattggcg gctttgcctc
     2041 tggctttttc aatggtcgtt tgaaattcat gaccaccaaa ggacccaata tctcagaccg
     2101 cacacgctgg ctgatggcat ttctcggtgg tgtgatcttc ttatatggcg cccgcatggc
     2161 tcgcggatgc acctctggtc aggcactatc gggtggcgct acactctcag cgggttcatg
     2221 ggtcgtgatg ttcgccatct ttggcggcgc atacgggctc gcttattttg tccgcaaatt
     2281 gtggaactaa ggagaataca atgaacttcc ctctctccgt ccttatcgta ccgagcgctg
     2341 aacatccctg gacctacctc gtctttttag tgatcgggat tgctttcggg tacaccctcg
     2401 aaatgtctgg ctttggtgac tctcgcaaac tggcggctca gttctacttc accgaattga
     2461 ccgtgctcaa agtgatgttc accgctattg tcacagccat ggtcttgctg tttggtgccg
     2521 ttgggctggg ctttttgg
//
LOCUS       DAOQNJ010000415         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_31710_length_2533_cov_5.400726, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000415 DAOQNJ010000000
VERSION     DAOQNJ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31710_length_2533_cov_5.400726"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLG52_10960"
     CDS             complement(<1..987)
                     /locus_tag="PLG52_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB42011.1"
                     /translation="MAVQASLQKLRPIWVERVSRELAAGEGVRSGFEEQLQRFYTLLE
                     QTIVTGDTAWLDPILFDWGHSPTQTNLEQADYHVSFVLDRMLSLTIDIAGDHLEDKDA
                     LELLAAVIPILAHSLGIVIRYEMETRVAHISKELGSVQEKLQNLDQNKSKFISVAAHE
                     LKTPLTLIEGYASMMVDLVQNSEQAVMKNYLSGVNTGIQRLRDIIDDMIDVSLIDNQL
                     LTLNIQPMWTSHLLNLVRNEFKKPIAERRQTLNVYDFNGSELMIYGDSERLYQALQNV
                     VSNAIKYTPDEGIITIDGRLLPGFIEITVKDTGIGISPENQSLIFDKFGQLGR"
     gene            complement(1117..2025)
                     /locus_tag="PLG52_10965"
     CDS             complement(1117..2025)
                     /locus_tag="PLG52_10965"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNB42012.1"
                     /translation="MNKRAVVLYLSIILGLVLIVTGWYAASFLTYTSESVPEGFSGDR
                     AYVDVQTQVDMGPRTPGSAGHAQIREWMRTELESAGWIVEVHESERLGHPIYNIIAKR
                     GKEPPQIILAAHYDTRFFADNDPDPAKHTEPVPGANDGASGVAVLLELARTLPVDTTP
                     TWLVFFDTEDNGRVEGWDWILGSRAFVEEVPIQPQAVVIIDMIGDADLNIFYEKNSDP
                     TIRAEIWSTAAELGYGKQFIQEEKYNMLDDHTPFVEKGIPAVDMIDFDYPYWHTVQDT
                     PDKVSAESLKAVGDTLWHWVVKHGEK"
     gene            complement(2025..>2533)
                     /locus_tag="PLG52_10970"
     CDS             complement(2025..>2533)
                     /locus_tag="PLG52_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB42013.1"
                     /translation="LAGSLYNIVGFQTNLKFPEQRRVLRMIPGLANAEFERYGQMHRN
                     TFIASPKLLRPTLQHITREDLFFAGQLTGVEGYMGNIATGLLAGINMARLIQNKALLT
                     LPKESMLGALCHYITHADLKDFQPMKANFGILPDLNLGKKISKREKGQAYADRASQSL
                     ETYLQESS"
BASE COUNT          618 a          605 c          623 g          687 t
ORIGIN      
        1 ccgcccgagc tgaccgaact tatcaaagat caacgactga ttctcaggag agattccaat
       61 gcctgtatct ttgacggtga tttcaataaa accaggcaat aaacgtccat caatcgtgat
      121 gatgccttcg tctggtgtgt acttgatagc gttggagacg acattttgca atgcttgata
      181 cagacgttcc gagtcgccat agatcatcaa ctcgctgccg ttgaagtcat agacattcaa
      241 ggtttgtctg cgttctgcga tgggtttctt gaactcattg cgaaccagat tcagcaagtg
      301 actggtccac ataggctgga tgttgagagt gagtagctga ttgtcgatca gcgagacatc
      361 gatcatatcg tcgatgatgt cacgcaaacg ttgaatgcca gtattgactc cgcttaggta
      421 gtttttcatc actgcctgct cagagttttg caccaagtcc accatcatgg aggcataccc
      481 ttcaataagg gtaagcggag tctttaactc atgggctgcc actgaaataa acttggattt
      541 gttctggtcg agattttgta atttctcttg aaccgacccg agttccttcg aaatatgtgc
      601 cacacgggtt tccatctcgt agcggatgac aatgccaagg ctgtgtgcca gaatcggaat
      661 gaccgccgcc aaaagttcca acgcatcttt gtcttccaag tgatcgcccg caatatcgat
      721 agtgagcgag agcatacggt caagcacaaa ggagacgtgg taatctgcct gttcgaggtt
      781 ggtttgtgtg ggagaatgcc cccaatcgaa caagatgggg tctaaccaag cggtatcgcc
      841 agtgacaatg gtctgttcga ggagggtata gaaccgttgc agttgctctt caaaacctga
      901 gcgcacgcct tccccagccg ccagctcacg ggagactctt tccacccata tgggtcttaa
      961 tttctgcaac gatgcttgga ctgccattcg cttatcgctt attccttcgt ttttaagcct
     1021 gtttttttat tgaagttgtc tgtgcaagaa agacttaacc tgccaaaatg tgcgatacac
     1081 agtaagtgta cgccgagtct ttccttttgg ctactactat ttttcaccat gtttaacgac
     1141 ccaatgccaa agcgtatcgc ccacggcttt taggctttct gccgagacct tatcaggcgt
     1201 gtcttgcacg gtatgccagt agggatagtc gaaatcgatc atatccacag caggaattcc
     1261 tttttcaaca aaaggggtgt ggtcgtccag catgttgtat ttttcttctt gaataaattg
     1321 tttgccatac ccaagctcgg cggctgtgga ccaaatctca gcccgaatgg tggggtctga
     1381 attcttttcg taaaagatgt tcaaatctgc atcgccgatc atatcaataa taaccacggc
     1441 ttgtggctga atgggtactt cttctacaaa agcgcgtgag ccgagaatcc aatcccagcc
     1501 ttctacacga ccgttgtctt cggtatcgaa aaagaccaac catgtgggtg tcgtatctac
     1561 tggcagggtg cgtgctaatt ctagcaatac tgccacacct gaagctccat catttgcgcc
     1621 ggggacaggt tctgtatgct tggcggggtc tgggtcatta tctgcgaaga agcgcgtatc
     1681 gtaatgagcg gcgagaatga tctgtggtgg ttcttttccg cgtttggcaa tgatattgta
     1741 aattggatgc cccagacgct ctgactcgtg gacctctact atccatccag cggactctag
     1801 ctctgttcgc atccattccc ggatttgggc atgtcccgcc gacccaggcg tacgcggacc
     1861 catatccacc tgggtttgca catccacata ggcgcggtcg ccgctaaagc cctcaggaac
     1921 agactcagac gtataggtca agaaagaagc ggcgtaccat ccggtgacga ttaacaccaa
     1981 gccaaggatg atggatagat aaagaacgac ggcgcgttta ttcatcaaga actttcttgt
     2041 aaataagttt ccaacgattg tgaagcacgg tctgcgtagg cttgcccttt ttctcgtttg
     2101 ctgattttct tgccgagatt caagtcaggc aaaattccaa agttggcttt catcggttga
     2161 aagtctttca aatctgcatg ggtgatatag tgacacaacg ccccaagcat cgattctttc
     2221 ggaagcgtga gcaaggcttt gttttgaatg agccttgcca tgttgatacc tgctaacaag
     2281 cccgtagcaa tgttgcccat gtagccttcc acaccggtga gttgacccgc gaagaaaagg
     2341 tcttcacgtg tgatgtgttg aagggtggga cgcaaaagtt tcggcgatgc aatgaaggtg
     2401 ttgcggtgca tttgcccata gcgctcgaac tccgcattgg caagaccggg aatcatgcgc
     2461 aatacacggc gttgttcggg gaatttcaaa ttggtttgga agccgacgat gttatataaa
     2521 cttccagcga gat
//
LOCUS       DAOQNJ010000416         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_31810_length_2529_cov_10.419968, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000416 DAOQNJ010000000
VERSION     DAOQNJ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31810_length_2529_cov_10.419968"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..849
                     /locus_tag="PLG52_10975"
     CDS             34..849
                     /locus_tag="PLG52_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNB42014.1"
                     /translation="MKMNAIVLAGGIPQPNDPLYAYSQGGSKALIDVAGKPMVQWIVD
                     ALAESKSVDQIIVIGLSQKSELTCSKPLHYISNQGRMLANIVAGSEKSLELKPNNDHL
                     LVCSADIPALRGDMVDWLVKTAMQTDDELYYGVCPREVMEARYPNSKRTYTKLKEMEL
                     CGADVNLVRISIIKEHMAVWEALIGNRKSPLRQAGIIGFDTLFQLATRQFTLESLVER
                     ASERIGIKGRAIVWDKAEPCMDVDKPHQLELMREDLTRQIRSEKAKARTAEKK"
     gene            846..1406
                     /locus_tag="PLG52_10980"
     CDS             846..1406
                     /locus_tag="PLG52_10980"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNB42015.1"
                     /translation="MSLRSILELDARLSNQMRVAEKPGILRNIAIFFAHSGDSWFWAA
                     ALIIIWFFSSSEWRHWETVEFFGLSGLAVVVLAVKFLVKRKRPEGEWGGIYRNTDPHS
                     FPSGHAARAFLIAVLASALAPTWLATILWVWAPLVALARVAMGVHYLSDIIAGAILGI
                     IVALVGLQFYQYLVDWFAGLTGFHFW"
     gene            1455..1853
                     /locus_tag="PLG52_10985"
     CDS             1455..1853
                     /locus_tag="PLG52_10985"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNB42016.1"
                     /translation="MKTHTFRLKPQQDLFDSIEEFVKQNNIEAGCILSSVGSLTHATL
                     RLANREHYNEYEGHFEIVSMTGTVSIHGSHVHVAISDGDGVTIGAHLVSGCKVYTTAE
                     IVLLELDDVVYKRELFENDSGYEELVVYKK"
     gene            complement(1899..2405)
                     /locus_tag="PLG52_10990"
     CDS             complement(1899..2405)
                     /locus_tag="PLG52_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42017.1"
                     /translation="MLILFSVLRFVHIIAGIFWAGGAILMNLIIGPAIGATGDAGKQF
                     VGHLMGKTIFSKFMLASGAITVLAGSILYGIDSNWFSSGWMMSPTGTGFGIGAISGII
                     ALVFGFLIGSTNGKLALLGMQIQGKPTNEQLAALGALRKRQVIVTNGNTIFILISIAL
                     MASARLFG"
BASE COUNT          688 a          637 c          535 g          669 t
ORIGIN      
        1 tttgaaccgc aattgcagga gttgaattaa cgtatgaaaa tgaatgccat tgtcctcgct
       61 ggaggcatcc ctcaacccaa tgacccgctg tatgcgtatt cacaaggtgg ttctaaagca
      121 ttgatcgatg tggcaggaaa acccatggtg caatggatcg tggatgcact tgccgaatcc
      181 aaatcggtgg atcagatcat cgtcattggt ctttcgcaaa aaagtgagtt gacctgttcc
      241 aagccgctgc actacatctc aaatcaggga cgtatgttgg caaacatcgt tgccggctct
      301 gaaaaatcgc ttgagcttaa gccaaacaac gaccatctac tcgtctgctc tgcagacatc
      361 cctgccttgc gcggtgacat ggtggactgg ctcgtcaaaa ccgccatgca aaccgatgat
      421 gaactctact acggtgtttg tccgcgtgaa gtgatggaag cccgctaccc taactccaaa
      481 cgcacctaca ccaaattaaa agagatggaa ctttgtggcg cagatgtcaa cctcgtccgt
      541 atcagtatca tcaaagaaca catggcagtt tgggaagccc tcatcggcaa ccgtaagagt
      601 cccttgcggc aggctggcat tattggcttt gacaccctct ttcaacttgc tacccgccaa
      661 ttcacccttg aaagtttggt cgaacgtgca tcagaacgca tcggcattaa agggcgagcc
      721 atcgtatggg ataaagctga accctgcatg gatgtggata aaccccatca gttggaattg
      781 atgcgcgaag accttacacg tcagatccgt tctgaaaaag ccaaagcaag gacggcagag
      841 aaaaaatgag tctgcggtct atccttgaac tcgatgcccg actttccaat caaatgcgcg
      901 ttgcggaaaa gccgggcatt ttgcgcaaca tcgccatctt ctttgcccac tccggcgact
      961 catggttttg ggcagcagcc ctcattatca tctggttctt tagcagctcc gaatggcggc
     1021 actgggagac cgtagaattt tttggtcttt ccgggttagc agttgtcgtg ctggcggtta
     1081 aatttctcgt caaacgcaaa cgccctgaag gcgaatgggg aggcatttac cgcaatacgg
     1141 atccacactc cttcccatca ggacacgccg cccgcgcctt cctcatcgca gtccttgcct
     1201 cggctcttgc tccgacctgg cttgctacta tcctttgggt ttgggcgccg ctggtagctt
     1261 tagctcgcgt agccatgggc gtgcattatc tctccgatat cattgctgga gccattctcg
     1321 gcatcatcgt tgccttggtg ggcttgcagt tctaccaata tctagtcgac tggtttgccg
     1381 ggctgacagg cttccatttc tggtaatctt ttcgtgcgag agctaaactc tcgcacgaac
     1441 tttttataaa acctatgaaa acacatacct ttcgtcttaa acctcaacaa gacctctttg
     1501 actccattga agagttcgta aaacaaaaca atatcgaagc cggctgtatc ctttcgagtg
     1561 taggtagcct aacccatgct actttgcgcc ttgcaaaccg cgaacattac aacgagtacg
     1621 aagggcattt tgaaattgtc tccatgaccg gcaccgtatc gattcatggt tcacacgttc
     1681 atgtcgctat ctctgatgga gatggcgtta ccattggtgc tcacctcgta agcggctgta
     1741 aggtctacac caccgcagag attgtgttac tcgaattaga tgatgtagtt tataagcgcg
     1801 aattattcga aaatgactcg ggctacgaag aattggtcgt gtataaaaaa taaaagacag
     1861 acacccttga tgggtgtctg tcttttattg caacgcattt atccaaataa tcgtgcagac
     1921 gccatcaaag caatcgatat aagaatgaaa attgtgtttc cgtttgttac aataacctgg
     1981 cgtttgcgca atgcacctaa agccgctaat tgctcattcg taggtttacc ttgaatctgc
     2041 atgccaagta aagctaattt tccatttgta cttccgatta agaacccaaa gactaacgct
     2101 ataatcccag aaatagcacc gattccgaag ccagtgcctg tcgggctcat catccatcca
     2161 gagctaaacc aatttgaatc aataccgtat agaatacttc cagccaaaac ggttattgct
     2221 cccgaagcaa gcataaattt cgaaaatata gtttttccca ttaagtgacc aacaaattgt
     2281 ttgcccgcat ctccagttgc gccaatggca ggtccaataa ttaagttcat caaaatagcg
     2341 ccacctgccc agaaaattcc tgcaataata tgtacaaatc gtaaaacaga aaacaaaatt
     2401 aacatggtac tctcctttaa aatttatcaa atctaataat aagatatatt ttatccattt
     2461 taattcacaa aacaaaatcc ttctaaagtc ttgctccttc atgtagaata accccatgcc
     2521 cgatttgtc
//
LOCUS       DAOQNJ010000417         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_31853_length_2527_cov_9.705097, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000417 DAOQNJ010000000
VERSION     DAOQNJ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31853_length_2527_cov_9.705097"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..758
                     /locus_tag="PLG52_10995"
     CDS             87..758
                     /locus_tag="PLG52_10995"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNB42018.1"
                     /translation="MLRDRISPNISEFLRYLASHPEADDKLPSLNELSRELNISLASL
                     REQLEVARALGFVEVRQKTGTRRLTYSFTPAIRQSLGYALALNDEHFRKFAEMRNHLE
                     AAYWYEAVKQLTDEDKKELNTIITRAWEKLQGTHVQVPHEEHRKLHLTIYKNLDNPFV
                     IGVLEAYWEAYEAVGLNVFTGGYEYLKEVWQYHQRMVESICSGKFEVGYEALVRHTDL
                     LYHRP"
     gene            833..>2527
                     /locus_tag="PLG52_11000"
     CDS             833..>2527
                     /locus_tag="PLG52_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNB42019.1"
                     /translation="MNKIVNDFSITVGTKNGSGSSTANNTILRSIFKMGIPVSGKNLF
                     PSNIQGLPTWYTIRVNKDGFIARKETSDIVIAMNPDSFAKDLASVTPGGAFFYADDIK
                     QPITRVDIAIYPMPVKTIVKNDPNVPTDFRELVGNMVYVGVLAQVIGMDMEAIRAALT
                     FHFKGKQKPIDMNLNAVKAGAEWAAANLEKKDPFYLEHMNKTEGLIMSDGNTAGAIGS
                     IFGGVQFAGWYPITPASSLAEALNDYLPVLRKREDGKHTYAVVQAEDELAALGMAIGA
                     GWSGLRSMTSTSGPGLSLMTEFAGLAYYAEVPVVIWDVQRIGPSTGLPTRTSQGDLSF
                     TAFIGHGDSHSVILLPGDVSECFEFGWKSFDIAERLQTPIFVLSDLDMGMNQWMSKPF
                     EYPEVPMDRGKVLWEKDLEEIKGNWGRYLDKDGDAIAYRTIPGNKHPLSSYFTRGTGH
                     DEYAKYTEDSGMYMKNMERLARKQQTAKEFVPAPVLHAKKGATVGILAFGSTENAIFE
                     AQAQLEAENGIKSELLRLRAIPFTKEVDAFIKKYDQIFVVEMNRDGQLLQLLYTEYP"
BASE COUNT          655 a          580 c          606 g          686 t
ORIGIN      
        1 cttgaattaa ctaaactctt aaccaaaaat ccttgaccga aggctttcac gggcgtaaaa
       61 tacgattaat cggattaatc aattttatgc tacgtgaccg tatttcaccg aacatttccg
      121 aatttcttcg ttatctggcg tctcaccctg aagcagatga caagctcccc tctttaaacg
      181 aacttagccg agagttgaat attagccttg catcactacg cgaacaacta gaagttgctc
      241 gcgcgcttgg ctttgtcgaa gttcgtcaaa agactggaac gcggcgattg acttattcct
      301 tcactccagc aatccgccag agtttgggat atgccttggc actgaatgat gagcattttc
      361 ggaaatttgc agaaatgcgt aatcatcttg aagctgctta ttggtatgaa gcggttaagc
      421 aattgacgga tgaagataaa aaagaactca acaccatcat tacacgcgct tgggagaaac
      481 ttcaaggcac gcatgtgcaa gttccccatg aagaacaccg taagttacac cttaccattt
      541 acaaaaactt ggataaccct tttgtgatcg gtgtattaga agcgtattgg gaagcctatg
      601 aagcagtggg tttaaatgtg ttcacgggcg ggtatgaata tttgaaagaa gtgtggcaat
      661 accatcagcg tatggtcgag tcaatttgta gtggcaagtt tgaggtcggg tatgaggcgc
      721 ttgtacgtca tactgacctt ttgtatcatc gtccctaggt cattttcgtc gataggcaca
      781 gatgaggcta taaccgtctc tgttcatcga cctaattttt atgaggagtt ccatgaataa
      841 gatagtgaac gatttttcga tcaccgttgg tacgaagaac ggttccggca gttccactgc
      901 caataacacc attttgagat ccatctttaa gatgggcatc cccgtctctg gcaagaacct
      961 gttcccttcc aatatccaag gtctgcctac gtggtacacc attcgtgtga ataaggatgg
     1021 tttcatcgcc cgtaaggaaa ccagtgacat tgtcattgcg atgaaccccg attcgttcgc
     1081 aaaggacctc gcatctgtta cgccaggtgg ggcttttttc tacgcagacg atatcaagca
     1141 gcccatcacc cgagtggaca ttgccattta cccaatgccg gttaagacaa tcgtcaagaa
     1201 cgacccgaat gtacctaccg attttcgtga attggttggc aacatggtgt atgtaggcgt
     1261 gcttgcacag gttatcggca tggatatgga agccatccgt gctgcgttga ccttccattt
     1321 caagggcaag caaaagccaa ttgatatgaa cctcaatgca gttaaagctg gtgctgaatg
     1381 ggctgctgca aacctcgaaa agaaagatcc cttctatctt gagcacatga ataagactga
     1441 aggcttgatc atgtcagatg gcaatacagc tggagcgatt ggttctatct ttggtggtgt
     1501 tcaatttgct ggttggtacc ccatcacgcc tgcttcttct ttggcagaag ctttaaatga
     1561 ttacttgcct gtgcttcgta aacgcgaaga tggcaagcac acttatgctg ttgttcaggc
     1621 agaagatgaa cttgcagcgc tcggtatggc aattggcgca ggttggtctg gtctgcgttc
     1681 gatgacttca acttctggac cgggtctttc tctcatgacc gagtttgcag ggttggcata
     1741 ctacgctgaa gttccagttg tgatctggga tgtccagcgt atcggtccca gcaccgggtt
     1801 gcccactcgc acctcgcaag gtgacttgag cttcaccgcc tttattggtc atggtgactc
     1861 acattctgtt attcttttgc caggtgacgt atctgaatgt tttgaatttg gctggaaatc
     1921 ttttgatatt gcagaacgcc tccaaacccc gatcttcgtt ttgagcgatc tcgatatggg
     1981 tatgaaccag tggatgagta agcccttcga atatcccgag gtcccgatgg atcgcggcaa
     2041 agtgctttgg gaaaaagacc tcgaggaaat caaaggcaat tggggtcgtt acctcgataa
     2101 ggatggcgat gccattgcat atcgcaccat tccgggcaat aagcacccgc tcagctcata
     2161 cttcacccgt ggaaccggtc acgatgaata tgccaagtat acagaagact ctggtatgta
     2221 catgaagaac atggaacgcc tcgcccgcaa acagcaaacc gccaaggaat ttgtccctgc
     2281 accggtgttg catgcaaaga aaggcgcgac ggtcggtatc ctcgcttttg gctccaccga
     2341 aaatgcgatc ttcgaagcac aggcacagct tgaagctgag aacggtatca agtctgagtt
     2401 gttgcgcttg cgcgccattc ctttcactaa agaagtagat gcctttatta agaaatatga
     2461 ccagatcttt gtggtcgaaa tgaaccgtga tggacaattg ctccagttgt tgtacactga
     2521 atatccg
//
LOCUS       DAOQNJ010000418         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_31922_length_2524_cov_7.761037, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000418 DAOQNJ010000000
VERSION     DAOQNJ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31922_length_2524_cov_7.761037"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLG52_11005"
     CDS             complement(<1..470)
                     /locus_tag="PLG52_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42020.1"
                     /translation="MKNIISRVVLAVLVASLAFAAIPVTSAYAADDTPPAKGELTDEK
                     LEEIWARASQAFGRLGKAFEDTDAHIAKIQSMIDKAAANGKDVSSLQSALDAYKTALV
                     ASKPAYEQLAATMESHAGFDANGKVTDSETAKATLKQVRDEMKALKESMGGTFKA"
     gene            complement(600..2267)
                     /locus_tag="PLG52_11010"
     CDS             complement(600..2267)
                     /locus_tag="PLG52_11010"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNB42021.1"
                     /translation="MKKISLFLFIAILFSACAPATEAPASPAETTVPAQPTSTQAPVA
                     DALWISPATPDVLREAATNSGVPIVNDAESATQKLDIADSGSLWIYALVAPFPTVTDG
                     ISSADLISAWQNGSSLTGNGQPLLMAESTLRAFTALWGEPASGTVSIVASDQLLTTAW
                     TQPFSWAIIPFEEIQPKWKVLSVDGQSPIHKDFDANLYPLKLSFGLTNSNIELPATNR
                     DASKLATVVLTGVTALVRATAFTMENKGITRPGELVYNWLHDADVLHISNEVPFDPDC
                     PYPTPGYKNFILCSDPKYMELLTYVGTDIVELTGDHFADRGTQAMLDTLQMYRDNNIP
                     YYGGGANEEEGRKPVLMNVNGNKIAFMGCNGKVSYVFVKATDTKPGAADCDYEFFTQQ
                     VKDVAAQGYMVIFTFQHEECYHHGPCYKHEEGFHAVADAGATIVSGSQAHYPHLMEFR
                     GSTFIHFGLGNLFFDQMTYELPDGSVIDETRREFIDRHVFYDGKYLGTELLTAMLEDF
                     SRPRPMNERERAQFLSEYFAYSGWTELQPTPIPQPTVTLTPIILPTP"
BASE COUNT          638 a          599 c          639 g          648 t
ORIGIN      
        1 gctttgaacg tgccacccat cgactctttg agggctttca tttcatcgcg cacttgctta
       61 agtgtagctt tggctgtctc tgagtcggtc actttgccat tggcatcaaa accagcatga
      121 gattccatgg tggctgccag ttgttcatat gcaggcttgg acgcaaccaa tgcagttttg
      181 taagcatcaa gggcagattg caagctagac acatctttac cattggctgc cgctttatcg
      241 atcatggact ggatttttgc gatatgggca tcggtatctt cgaaggcttt ccccaaacgc
      301 ccaaaagcct gagaggcgcg cgcccagatt tcttccagct tctcatcggt caactcaccc
      361 tttgcaggcg gagtgtcatc cgcagcatac gcgcttgtga cggggattgc cgcaaatgcc
      421 agcgaagcaa ccaatactgc caacaccact cttgaaatta tatttttcat cgaaacctcc
      481 ttcaggttac gatgaaattc tagatgccag cggttagaag agtatggcgg ggatgtaaaa
      541 aggttgtaaa ttttataaag agctccgagg ttttgaaaac ctcggagctc ttggttcgcc
      601 tatggagttg gcaaaataat cggcgtcaat gtgacagttg gctgtggaat gggcgtgggc
      661 tgcaattctg tccagccgct gtaggcaaag tattcagaaa ggaattgcgc ccgttcacgt
      721 tcgttcatcg ggcgcggacg ggagaagtct tcgagcatgg cggtcagcaa ttcagtgccg
      781 agatatttgc catcgtagaa cacatggcgg tcgataaatt cacggcgggt ttcatcaatg
      841 accgaaccgt cgggcaattc atacgtcatt tgatcgaaga acaaattgcc caaaccaaaa
      901 tggataaaag tactgccgcg gaattccatc aaatgcgggt aatgcgcctg gctgccactc
      961 acaatcgtag cgccagcatc cgctacggcg tgaaaacctt cttcatgctt gtaacaaggt
     1021 ccatggtgat aacactcttc atgctggaaa gtaaagataa ccatatagcc ttgcgccgct
     1081 acatctttga cttgttgcgt gaagaattca taatcacagt ctgcggcacc gggtttggta
     1141 tcggtcgctt tcacaaagac atacgaaact tttccgttac agcccataaa cgcaattttg
     1201 tttccattca cattcattag gaccggtttg cgcccctcct cttcattcgc gccgccgcca
     1261 taatatggaa tattgttatc acgatacatc tgcaaggtat ccaacatggc ttgcgtgccg
     1321 cggtctgcaa agtgatcacc ggttagttcc acaatgtcag tgcctacata ggtaagcaat
     1381 tccatatatt tcggatcact gcacaaaatg aaatttttat agcccggggt tgggtatggg
     1441 caatcggggt caaaaggtac ttcgttgctg atatgcaaca catctgcatc atgcaaccag
     1501 ttatagacga gttcgccagg acgagttatc cctttgtttt ccatcgtgaa tgccgtagca
     1561 cgcacaagtg cggtcacacc ggtcaacacc acagttgcca attttgaagc atcacgattc
     1621 gtagcaggca attcaatgtt tgagttcgtc agcccgaacg aaagtttcaa cggatacaag
     1681 ttcgcatcaa aatctttatg gatcggcgat tgaccgtcta cgcttaagac tttccacttt
     1741 ggttggattt cttcaaaggg gatgatcgcc caactaaaag gctgtgtcca cgctgtggtt
     1801 aatagctggt cagatgcaac gatgctcact gtgcccgaag cgggttcgcc ccacagggca
     1861 gtgaacgccc tcagagtgga ttctgccatg agcaacggct gaccattccc ggttaaggaa
     1921 gagccgttct gccaggcgct gatcaggtct gcggacgaga taccatctgt caccgtaggg
     1981 aaaggtgcga cgagggcata aatccaaaga gagcctgaat cggcaatgtc taatttttga
     2041 gtggcgcttt cagcatcatt cacgattggc acgccagaat ttgtggcagc ttcacgcaag
     2101 acgtcaggcg tggcggggct aatccacaac gcatctgcaa cgggtgcttg tgtggaggtt
     2161 ggttgagcag ggacggttgt ttcagcgggc gaggcgggtg cttcagtagc aggcgcacaa
     2221 gcagagaata gtattgcaat gaagaggaat agtgatattt ttttcatttg taatccatgg
     2281 cgcaaaaaag tacgaagcac agagcgcctc gtacttgtaa acttaattga ttaaacgggc
     2341 gtgagcgtta gtttacaccc agtttggttg ttagactacc acctttgcca acgtgtaaga
     2401 cttgagcgcc tcttcatcga cagtgacgcc caaaccgggt ttgtttggta cgtcgatagt
     2461 tgaatctgaa ttgagaacga aacgctcatg cgtgatatcg cgagtgtagt aacggtcaga
     2521 tgcg
//
LOCUS       DAOQNJ010000419         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_31923_length_2524_cov_6.884164, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000419 DAOQNJ010000000
VERSION     DAOQNJ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31923_length_2524_cov_6.884164"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1183)
                     /locus_tag="PLG52_11015"
     CDS             complement(11..1183)
                     /locus_tag="PLG52_11015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB42022.1"
                     /translation="MNTSSQNLTSILLTAICFFAFFIFGTTDNLKGPTLPAMLAELNI
                     DYGTGGNIFFSEYVGFLIATLITGILADRFGLKIVFILAGIALVFGVGGYSSFSTPFL
                     LSTSLFIVGLGLGAFELGPNAVIVSLYHEQKGLYLNLMAVMHGLGSMIAPLVAGYLLS
                     MDVNWRTIYRFDLLLIAIFLVASLLVRFPKSEEKASLDFRAIPTVAFKHTLPFYYGAM
                     LFYVAAEIGLASWMVVYLQEARSLNETASNNYLAIFFGMLMLGRFFGSFFVKRVGYLR
                     SILFATLLATASIALGIFTNLVIFIPITGFFFSIIFPTLTAAVSDAEQENINTILGTL
                     FTFSGIGGMIGPWLVAWSSKLFGLQVGFSSIILLTGSTLVFAFILNQRSNTNGQRS"
     gene            complement(1256..2461)
                     /gene="gguB"
                     /locus_tag="PLG52_11020"
     CDS             complement(1256..2461)
                     /gene="gguB"
                     /locus_tag="PLG52_11020"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNB42023.1"
                     /translation="MNFFSTARKLLQQNIREYGMYIALFVIMVYFTIMTEGTFISSRN
                     IVNLVNQTGYIAVLAIGMTLVIIIRHIDLSVGFLAGFLGAIAAIAMADPKVVAAGMSL
                     PVYIVVPMTLGLGIVAGLITAFLVAQMKIPSFVASLTGWLIYRGAILVVTESTGTIII
                     NNEAFNAIGNGFIPDIPDIGILPGVHKLTLLLGIFAIVAMIFSELNERRKKQSYNFET
                     LPTEMFVIKLVFISGIIGTITWILAGYQGLSWTAVVVLVVTLIYHFVMSQTVLGRHIY
                     AVGGNPDAAELSGISVKRLVYIVFGSMGMLSALSGILFASRLQSATTTAGNLFELDAI
                     AAAYVGGVSAAGGVGKVLGSIIGALVMTSLTSGMNLMGIGISYQYMVRGAVLAAAVIF
                     DVATRNTGK"
BASE COUNT          782 a          551 c          634 g          557 t
ORIGIN      
        1 caatccccaa ttaagaacgt tgtccatttg tgttactcct ttggttgaga atgaaggcaa
       61 agaccaaggt tgagcctgtc aatagaatga tggatgaaaa accgacttgc aaaccgaaga
      121 gtttactgct ccacgccacc aaccacggac caatcatccc gccgatgcca gaaaaggtaa
      181 atagcgtacc gagaatggta ttgatgtttt cttgttcagc atcagaaacg gcggcggtga
      241 gggtggggaa gatgattgaa aagaaaaagc cagtgattgg aatgaagatc acaaggttgg
      301 taaaaatgcc aagcgcgatg cttgccgtgg caagaagtgt ggcaaataaa atagagcgca
      361 aatagccgac gcgttttaca aagaaactgc cgaagaaacg tcccaacatc agcatgccaa
      421 aaaagatggc gagataatta ttgcttgctg tttcgtttag gctgcgtgct tcttgtaaat
      481 agacgaccat ccaggatgcc aaaccaattt cggctgcaac ataaaatagc atggctccat
      541 agtaaaacgg caatgtgtgt ttgaatgcaa ccgttgggat ggcgcgaaaa tctaacgagg
      601 ctttttcttc tgacttcgga aagcgtacta acaggctggc aacaagaaaa attgcgatga
      661 gcagtaaatc aaagcgatag atcgtccgcc agttgacatc catacttagg aggtatcctg
      721 caactaaagg cgcgatcatg gagccaagtc cgtgcatgac cgccatgaga ttaagatata
      781 agcctttttg ttcatgatac aaactgacaa taaccgcgtt gggaccaagt tcaaaagctc
      841 ccagcccaag cccaacgata aagagagagg ttgataaaag gaacggtgta ctgaatgagc
      901 tgtatccgcc aactccgaaa acgagagcta tccccgcaag aatgaaaaca attttgagac
      961 caaagcggtc tgccagtatg ccggtaatga gtgtggcaat taagaagccg acatattcac
     1021 taaaaaagat attcccacct gtgccatagt caatgtttag ctctgctaac atggctggca
     1081 atgtaggccc tttgaggtta tcggtggtgc caaagataaa aaaagcaaag aaacagatgg
     1141 cagtgaggag aatggaagta aggttttggg aagatgtatt catttggtga ttatagaaca
     1201 aaaagagaca gggcttgtac acaagccctg tctctcattt tgagtgaagc gaaaattatt
     1261 tgccggtatt gcgggtggct acgtcaaaga tcacagcggc ggcgagcact gctccgcgaa
     1321 ccatgtattg atatgagata ccaatgccca tcagattcat gccgctggtg agcgaggtca
     1381 ttacgagcgc accaatgatg gagccaagca ctttacccac accgcctgca gcagagacac
     1441 cgcctacata cgccgcggca atggcatcca attcgaagag attgcctgca gtggtagtgg
     1501 cagattgaag gcgagaggca aacaaaattc cagaaagagc cgagagcatg cccatcgagc
     1561 cgaagacgat atagaccagt cgcttcacgc tgatgccgct caactcggcg gcatcggggt
     1621 taccgccgac cgcataaatg tgcctgccga gcacagtttg cgacatcaca aaatgataga
     1681 taagggtgac gaccaataca actacggccg tccatgagag accttgatac ccagccaaaa
     1741 tccaagtgat ggttccgata atgcccgaaa taaagaccag cttgatgaca aacatttcgg
     1801 taggcaaggt ttcaaagttg taggactgtt tctttctgcg ttcgttgagc tcgctgaaga
     1861 tcatggcaac aatcgcaaaa ataccgagaa gtaatgtgag cttatgaacc ccagggagaa
     1921 tgccaatatc tggaatatct gggataaagc cattgccaat agcattgaag gcttcgttat
     1981 taatgatgat cgtgccggtg ctttctgtaa ctaccaaaat ggcaccacga tagatcaacc
     2041 aacctgttag cgaagcgacg aaagaaggga tcttcatttg agcgacaagg aaggcggtga
     2101 tcaacccggc gacaatgccc aagccaagtg tcatgggcac aacgatgtac acaggcaggc
     2161 tcattcctgc ggcaacaact ttagggtctg ccatggcgat tgcggcaatg gcgccgagaa
     2221 aaccggcgag aaagcctact gatagatcga tatgacgaat gataatgacc aaggtcatac
     2281 caatggcgag cacggcaatg taacctgttt ggttaaccaa gttgacgatg ttgcgcgatg
     2341 aaatgaacgt gccttctgtc atgatggtga agtaaaccat gatcacgaaa agcgcaatgt
     2401 acatgccata ttcgcggatg ttttgttgca aaagttttcg tgcggtactg aagaaattca
     2461 taagggtatt ctcctagttc gttgccaaat gcatgatctt ttcttgcgtg gcttcctgag
     2521 caga
//
LOCUS       DAOQNJ010000420         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_32059_length_2518_cov_9.505481, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000420 DAOQNJ010000000
VERSION     DAOQNJ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32059_length_2518_cov_9.505481"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..>2518)
                     /locus_tag="PLG52_11025"
     CDS             complement(77..>2518)
                     /locus_tag="PLG52_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB42024.1"
                     /translation="LVESNQRFAQLTRHIHEVFWVSEEVGKLEYVSPAFEYIFGFPAE
                     DLYQGKDYIDLVHPEDRQIVLDSFEYDMRGENSLFEYRIIRPDGEIRWVSDRSYPILG
                     EHGELIRTVGITTDITEQKHSEAERRELTSILEDAEQQVGLASWSQDILTGKSKWSPY
                     MFTLFGFDPAEGIPSMPDYLERIHPEDRELLGSALFKMMQGEETSEMFTYRTNPHYGE
                     MRFLKPTYRTEKDANGRVIKFLGTVLDITEQKRSATELERRQKLLETVILAGKNITSI
                     IDFDKCLQEIHRNIISNLGFERVGLFIYDPKRHYVQGTYGTDRNGNPVSNDWYYEQIN
                     ENSEWQVALESPTGIAVIDDYGLARNPPPDNEMYGVKQHITLAAWSGDTPVALISVDN
                     LLTQKPITPADIEALQLFAGYAGLAIENARLHTGLENLIEKRTDELTQSQKSLQLFLD
                     TANDLIQSLDESGRYIYVNQSWCRTLGFTLEEASKLDMLQVVDKEYQDHCLNIFRNMM
                     ADGVSQMIEVGFRTKQGHLVIVEGSISIQTRSDGKRVTNGFFRDVTERKQAEIAMRHA
                     NLEMEKALRMKDEFMANMSHELRTPLNAILGLSESLLEETVGSINSRQHKYLNTISES
                     GRHLLELINDILDLAKIESGQIHLASNSVNIKTLCEASLRIVDQLAKSKKQAMSLEVA
                     DEIKFVQADERRLKQMVVNLLSNAIKFTPENGQVGIRALIDASRTFLNIEVWDTGIGI
                     KADDLSHIFQPFIQLNSGLARESSGTGLGLALVAQMARLHGGSITVRSEIGKGSSFIL
                     SLPWTPELPDAPQEQ"
BASE COUNT          558 a          576 c          616 g          768 t
ORIGIN      
        1 tcacgacatg aagttttatt tgattggttt cgatccattc gcttggcatt ggcgcgagta
       61 taacggaaaa tgccaattat tgctcctgag gggcatctgg cagttctggc gtccagggca
      121 agcttagaat gaaactgctg ccttttccaa tttcgctgcg aactgtaata ctgcccccgt
      181 ggaggcgagc catttgtgcc accaatgcca accccagccc tgtgcccgat gactcacgcg
      241 ccaaaccaga atttaattga atgaagggtt ggaagatgtg tgagaggtca tcagctttga
      301 tgccgatgcc tgtatcccag acttcaatgt ttaaaaaggt gcgtgacgca tcgatcaatg
      361 cccgtatccc tacttgaccg ttctcgggcg taaatttaat cgcattgcta agcaagttga
      421 caaccatttg ttttaggcga cgttcatctg cttggacaaa cttaatctca tcggcgactt
      481 cgagactcat ggcttgtttt ttgctttttg ccagttggtc aacgatgcgg agagaggctt
      541 cgcacaaggt cttgatgttt acagagttgg aggctaagtg gatttgccct gattcgatct
      601 ttgccagatc cagaatgtca ttgatcaatt ccagtagatg acgtccgctt tcgctaatgg
      661 tgttgagata tttatgttgg cgggaattga tggagccgac agtttcttcg agcaggcttt
      721 ctgaaagtcc taaaattgca ttgagcgggg ttcgaagttc atggctcata tttgccatga
      781 actcatcttt catgcgcaat gctttttcca tttcgaggtt ggcgtgtcgc atggcgattt
      841 cggcttgttt gcgttcggtc acatcacgga agaaaccatt ggtgacccgt ttcccgtcgc
      901 ttcgtgtttg aatgctgata ctgccttcca cgatcacaag atgaccttgc ttggtgcgaa
      961 agccgacctc aatcatctgc gacaccccat ctgccatcat atttctaaaa atgttcaaac
     1021 agtggtcttg atattctttg tccactactt ggagcatatc taatttgctg gcttcttcaa
     1081 gggtgaatcc taatgttcga caccaggatt gattgacata gatatagcgt ccgctttcat
     1141 caaggctttg aattaggtca ttggcggtgt ctaaaaacag ttgcagactt ttttggcttt
     1201 gcgttaattc atcggttcgt ttttctatca ggttttcaag cccggtatgt aggcgagcgt
     1261 tttcgatggc aagcccggca taccctgcaa acaattgcaa ggcttcaata tccgccgggg
     1321 taataggctt ttgggtcaat aagttatcga cagagatcaa agccactggc gtatctccag
     1381 accaggcggc tagagtgatg tgctgcttaa ctccatacat ctcattatct ggtggtgggt
     1441 tgcgggctag cccataatcg tcgatcacgg caatgcctgt tgggctttcc aaagcgacct
     1501 gccattcgct attttcgttt atttgttcgt aataccaatc attactgact gggttgccgt
     1561 tgcgatctgt gccgtaggtg ccttgtacat aatgtctttt tgggtcatag atgaataagc
     1621 ctacacgttc gaacccgagg ttgctaataa tgtttcggtg aatctcctgc aagcatttgt
     1681 cgaaatcaat aatggaggtg atgttctttc cggcaagaat gacggtttca agtagttttt
     1741 gccttcgttc caattctgtt gcactgcgtt tttgttctgt aatatccagc acggtgccaa
     1801 ggaattttat gacgcgccca ttcgcatctt tttcggtccg gtaggtgggt ttcaagaacc
     1861 gcatttctcc atagtgtgga ttggtgcggt aggtaaacat ttcggacgtt tcttcgcctt
     1921 gcatcatctt gaataaagcg gagccaagca gttctcgatc ttcggggtgg atgcgttcca
     1981 aataatctgg catgcttgga atgccctctg ccgggtcaaa cccgaacagt gtgaacatat
     2041 aggggctcca cttgcttttt cctgtcagaa tatcttgaga ccaacttgcc agcccaactt
     2101 gttgttcagc atcttccaga atggatgtta gttcccggcg ctcggcttct gagtgttttt
     2161 gttctgtgat atcggtcgtg attcctactg tgcgaatgag ttcgccatgt tccccaagga
     2221 tcgggtagga acggtctgag acccagcgaa tttcaccgtc tgggcgtatg atgcggtatt
     2281 cgaatagcga gttctcgccg cgcatgtcat attcaaatga gtcgagcacg atctggcggt
     2341 cttccgggtg taccaagtcg atgtaatctt ttccttggta caagtcttcg gctgggaacc
     2401 caaaaatgta ttcgaacgcc gggctgacgt attccagttt gcctacttct tcagagaccc
     2461 aaaacacttc gtggatgtgc cttgtaagtt gggcaaaacg ttgattgctt tcgaccag
//
LOCUS       DAOQNJ010000421         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_32399_length_2502_cov_8.704945, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000421 DAOQNJ010000000
VERSION     DAOQNJ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32399_length_2502_cov_8.704945"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLG52_11030"
     CDS             <1..197
                     /locus_tag="PLG52_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42025.1"
                     /translation="KWVREGILAKYEKASSQKPAASKKAAVKKTVAKAKAAPAKKVAV
                     KKSASKKPAKSKSAGKAKRK"
     gene            230..1285
                     /locus_tag="PLG52_11035"
     CDS             230..1285
                     /locus_tag="PLG52_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNB42026.1"
                     /translation="MSETLPMAGAPANVNSVGLSKNDYRGAPSTLCQGCGHNSISNQI
                     ISAMYEMNVLPEDVIKFSGIGCSSKSPTYFMNRSFGFNSLHGRMPSIATGALFGDAHM
                     KGIGVSGDGDSASIGMGQFKHAMRRNVNMVYIVENNGVYGLTKGQFSATAEKGLQLKK
                     QGENPYMPVDICMEGLISNASFVARSFAGNPKQVKELLKAAFAHKGIAVLDIISPCVT
                     FNNQENAYHSYTWGKDHEEPLHEISYIAPRSEIMLEREMNEGEIREVAMHDGSTVILK
                     NLEKGYDPTNRMLALQALEEAQQNNWLLTGLIYVNPSKPALTDIYNLVDTPLNRLTET
                     DLRPDRAMIDKINALMF"
     gene            1348..1752
                     /gene="arfB"
                     /locus_tag="PLG52_11040"
     CDS             1348..1752
                     /gene="arfB"
                     /locus_tag="PLG52_11040"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNB42027.1"
                     /translation="MKNLENEISLEYIRASGPGGQNVNKVATAVQLRFDVVNSPSLGS
                     DLKSRLIQLAGKRVNAEGVLILEAKRFRTQEANREDAIAKLYEMIRKASEKPKTRHKT
                     KPTKASKEERMKEKKKRGAVKKLRQTKARFEE"
     gene            1820..>2502
                     /locus_tag="PLG52_11045"
     CDS             1820..>2502
                     /locus_tag="PLG52_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB42028.1"
                     /translation="MHKHHTFAGLISGLTAASIWGGMYVVSKVVLEVIPPFTLLLIRL
                     IMGAAALGIVIYFRNRKSSTSTPVTKDIFWKSFLVGFVGYGISLGFQFVGTKLSTASN
                     GSLVTSATPAFVLLFAPFLLGEKPTTRRIIALVLSTFGVLAVIDPRTAELSPSLFWGN
                     MSLLAAALTWALYSVLVRKVSQTQDLLTSSTIMLLGGIPSSLAFSLWEINTQGVGEIT
                     LGIIGGLLFL"
BASE COUNT          685 a          594 c          602 g          621 t
ORIGIN      
        1 caaaatgggt tcgcgaagga attttggcaa agtacgagaa agccagcagc cagaaacctg
       61 ccgccagcaa aaaggctgca gtgaagaaaa ctgttgcaaa ggcaaaagca gctccggcga
      121 agaaagtggc ggtgaaaaaa tccgcaagca agaaacctgc taaatcaaag tccgctggca
      181 aggcgaagcg gaagtagtta tcggtgaatt tgaaaaggag taaataacaa tgagcgaaac
      241 tcttcccatg gctggcgccc ctgcaaatgt aaatagcgtt ggcttgagca aaaatgatta
      301 tcgtggcgca cccagcacct tgtgccaggg ctgtggtcac aactcgatct cgaatcagat
      361 catttcggcg atgtatgaaa tgaacgtgtt gcctgaagat gtgatcaagt tctcgggtat
      421 tggttgttct tccaaatcac caacctactt catgaaccgc agcttcggtt tcaactccct
      481 gcacgggcgt atgccttcca ttgcgacagg cgcgttgttc ggtgatgcac acatgaaagg
      541 cattggcgtt tctggcgatg gcgacagcgc cagcattggt atgggtcagt tcaaacatgc
      601 catgcgccgc aatgtgaaca tggtgtacat tgtggaaaac aatggtgtgt atggtctcac
      661 caaaggtcag ttctctgcga ccgctgaaaa agggttgcaa ttgaagaagc aaggtgaaaa
      721 cccatacatg ccggttgata tctgcatgga aggtctcatc tcaaatgcaa gctttgtggc
      781 tcgttctttt gcgggtaacc ccaaacaagt gaaagaactt ctcaaggcgg cttttgcgca
      841 caaagggatt gccgttctcg atatcattag cccttgtgtt accttcaaca atcaggaaaa
      901 tgcatatcac tcctacactt ggggtaaaga ccacgaagaa cctttgcatg aaatttctta
      961 tatcgcgccg cgtagtgaaa tcatgctcga aagagaaatg aacgaaggcg aaatccgcga
     1021 agttgccatg catgatggtt ccacagttat tctcaagaat ctcgaaaagg gctatgaccc
     1081 caccaatcgc atgctggcat tgcaagctct tgaagaagct cagcagaata attggttgct
     1141 caccggtttg atctacgtca acccatccaa acctgccctc acggatatct acaatcttgt
     1201 agatactcct ctcaaccgcc tgaccgaaac cgatctgcgc ccagaccgcg ccatgatcga
     1261 caagatcaat gccttgatgt tctagtttgc tagcacttca acccctctct gaaaagagag
     1321 gggtttttat tttataattt tgagagaatg aaaaatctag aaaacgaaat ctcgctggaa
     1381 tatatccgtg catcgggtcc gggaggacaa aacgtcaata aagttgcgac ggcggtacaa
     1441 ctgcgctttg atgtggtgaa ttccccatca ctcggctcag acttaaagag caggctgatt
     1501 cagcttgctg ggaaacgagt caacgctgag ggcgttttga ttcttgaagc aaagcgcttc
     1561 cgcactcaag aagcgaaccg cgaagacgcc atcgcaaagc tatacgaaat gattcgaaaa
     1621 gctagcgaaa agcccaaaac acgtcacaag accaagccca ccaaagcctc aaaagaggaa
     1681 agaatgaaag agaagaaaaa gcgaggcgcg gtaaagaaat tacgtcaaac caaggcacgt
     1741 tttgaagaat agattctata catgccctgc cctctaaaaa gtgcaatttt caagtgtggg
     1801 tattatataa ttgagggcta tgcataaaca tcacacattc gcaggtttga tctctgggct
     1861 cactgccgcc tcaatttggg gcggcatgta cgttgtcagt aaggtggttt tggaggtaat
     1921 tcccccattc accctgcttc ttatccgtct tattatgggg gcagcagcgc ttggaattgt
     1981 catttatttt cggaaccgaa aatcttcaac ctcaacgccg gttacaaaag atattttttg
     2041 gaagagcttt ctagttgggt ttgtaggcta tggcatttcg ctgggctttc aatttgtagg
     2101 gacgaagctt tccaccgcat cgaacgggtc tttggttacc tctgctaccc ccgcttttgt
     2161 tttgctcttt gccccattcc tgcttggtga aaaacctaca accagacgca tcattgcgct
     2221 tgtactttcc acatttggtg tgctcgccgt gattgacccg cgcacagcgg aactttcgcc
     2281 atcccttttc tggggcaata tgagtctatt ggcagcggcg ttgacatggg cactgtattc
     2341 ggtcttggtc cgtaaagttt cgcaaacgca agaccttctc acctctagca cgatcatgct
     2401 gcttggcgga atcccctcca gcttggcgtt ctcgctctgg gaaatcaata cgcaaggtgt
     2461 tggcgagatc acacttggca tcattggcgg attgttattc ct
//
LOCUS       DAOQNJ010000422         2500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_32452_length_2500_cov_7.010225, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000422 DAOQNJ010000000
VERSION     DAOQNJ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32452_length_2500_cov_7.010225"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLG52_11050"
     CDS             complement(<1..194)
                     /locus_tag="PLG52_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42029.1"
                     /translation="MENLQPNDVLAKAEALRNSLSTGFRDEIVKSLYSEAEKIAHRAV
                     KTADNKKYDFDQKIDRLVTSP"
     gene            complement(196..1032)
                     /locus_tag="PLG52_11055"
     CDS             complement(196..1032)
                     /locus_tag="PLG52_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNB42030.1"
                     /translation="MSNTKTILPTEHCETCPAFTQLEQMGIKVGDFDRVVALAGNPNT
                     GKSTLFNALTGLKQHTGNWPGKTVTRAEGGFEFNKLRYKLVDLPGTYSLLSASQDEEV
                     ARDFILFGQPNCTIVVTDATALERNLNLVMQVMEITENVVVAVNLMDEARRKGLEVDA
                     RSLSRDLGVPAIPVTARTGEGIHTLLGAVAEVIEGKVETKPLRVNGTPEFQNAVNELV
                     PMIKQLAPGIPNARWLAIRLLDGDARVQKAITSGELEEIVSRQYQQDAQFSKKMSVEG
                     RQ"
     gene            complement(1043..2038)
                     /locus_tag="PLG52_11060"
     CDS             complement(1043..2038)
                     /locus_tag="PLG52_11060"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HNB42031.1"
                     /translation="MYYTIAAITLLVLIFMPRIGLLALYKNYRTSQIREQVEDALKHL
                     LDRERQGRHASPESLGGTLDLQRARVTHLIQDMEAQGLVESNGNELHLTAKGERWALH
                     VVRAHRLWERYLADEARMPLNKIHDEAQKREHSLTEAQLNELDAALGHPTRDPHGDPI
                     PTREGKMNQTEGKPITAWQADRPARIVHLEDEPALAYQQILAAGLRLGQDIRVIEKTP
                     NRIVLSDGENEYRLAPAVAYNIHVAAIPESEMLKREAVPLFELEHDQRGEIVTLDDAV
                     QGFTRRRFLDLGLTPGTVIYPELKNFFGDPRAYRVRGTLIALRKDQAAQIWVKPI"
     gene            complement(2054..>2500)
                     /locus_tag="PLG52_11065"
     CDS             complement(2054..>2500)
                     /locus_tag="PLG52_11065"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNB42032.1"
                     /translation="RIFGLVLWLSPLVYLVNPVWMNWSKISLPAWSRWFGVVFGIFCV
                     FGIYWLFTSIGSGITPVSATRKEHKLATSGIYRWIRHPLYTVGSSFIVSFGLIADNWF
                     IIALGILAFTIMAIRTPKEEANLIEKFGDEYRDYMQRTGKFFPKFK"
BASE COUNT          581 a          606 c          625 g          688 t
ORIGIN      
        1 ggggaggtga cgaggcggtc tattttttga tcgaagtcat attttttgtt gtctgccgtt
       61 ttgacggcgc gatgtgcgat tttttctgct tcactataaa gggatttgac gatctcatcg
      121 cgaaagccgg ttgataaact attgcgtaag gcttcggctt ttgccaagac atcgttgggt
      181 tgtaagtttt ccatattatt gccgtccttc tacagacatc ttcttgctaa attgcgcatc
      241 ttgctgatat tggcgcgata cgatttcttc aagttcgccg ctggtgatgg ctttttgtac
      301 acgcgcatca ccatccaaca agcggatcgc caaccagcgt gcgttgggaa taccgggagc
      361 cagttgtttg atcatgggca ccagttcatt aacggcattc tgaaattcag gagtgccatt
      421 cacgcgcaat ggcttggttt ccactttgcc ttcgatcact tcagcaacag cacctaataa
      481 agtatggatt ccttcgccag ttcgtgcagt gactggaatg gcaggtacgc ccaagtcacg
      541 cgagagagaa cgagcatcca cttcgagacc tttgcggcgg gcttcgtcca tgaggttgac
      601 agcaacaact acattctcag tgatttccat tacttgcatc acaaggttta ggttgcgttc
      661 aagcgctgtg gcatcggtca caacaatggt gcagtttggt tgaccaaaca gaataaaatc
      721 gcgtgccact tcttcatctt gtgaagcaga caataaagaa tacgtgccgg gcaggtccac
      781 aagtttgtag cgcaatttgt tgaattcaaa gccgccctcg gcacgggtga cggtcttgcc
      841 aggccagttg ccggtgtgct gcttgagccc ggtcaacgca ttgaataaag tagatttgcc
      901 agtgtttggg ttgccagcta atgcaaccac acggtcgaag tcgccgactt tgattcccat
      961 ttgttcgagt tgcgtaaatg cggggcaggt ctcgcaatgt tcggtgggga ggatggtttt
     1021 tgtattagac atgattcgtt ccttatatgg gttttaccca aatttgtgct gcctgatctt
     1081 tacgtaatgc gatcaatgtg ccacgaacgc gataagcgcg tggatcgccg aagaaatttt
     1141 tcagctcagg gtaaatcacg gttccaggag tcagcccgag gtccagaaag cggcggcgcg
     1201 tgaagccttg cacggcatca tccagagtca caatttcgcc acgctgatcg tgctccaatt
     1261 caaataacgg aacggcttca cgctttagca tctcgctctc aggaattgcc gccacatgaa
     1321 tgttatatgc cacagccggc gcaagccgat attcattttc gccgtcactc aacacaatgc
     1381 ggttcggtgt tttttcaatc acgcggatgt cttgccccaa gcggagccct gccgccagga
     1441 tttgttgata cgccagagcg ggttcatcct ctaagtggac gattcttgcc ggacggtctg
     1501 cctgccacgc cgtaatgggc tttccttctg tctgattcat tttcccctca cgcgttggaa
     1561 tcgggtcgcc atgtgggtcg cgagtggggt gacccaatgc ggcatcgagt tcatttagtt
     1621 gtgcctcagt gagagagtgt tcacgtttct gcgcttcgtc atggatcttg ttcagtggca
     1681 tgcgtgcttc atctgccaag taacgctccc ataaacgatg tgcacgtaca acatgcaatg
     1741 cccagcgttc gcctttggca gtgagatgta attcattccc attcgactca accaaacctt
     1801 gagcttccat atcttgaatg agatgggtga ctctggcgcg ttgaaggtct agggtgcctc
     1861 ccaaagattc tggagaggcg tgccttcctt gtcgttcacg gtctagaagg tgtttcagag
     1921 catcttccac ctgctcacgg atttgactgg tacggtaatt tttataaagc gccaataatc
     1981 ctatgcgcgg catgaagatg aggactaata atgtaatcgc agcaatggtg taatacatag
     2041 tttttttcct tgcttatttg aatttcggga agaattttcc cgtccgttgc atatagtcgc
     2101 gatattcatc accgaatttc tcaatgaggt ttgcttcttc tttcggcgtg cggatcgcca
     2161 tgatcgtgaa tgccaatatg cctaatgcga tgatgaacca gttatctgct atgagaccaa
     2221 acgagacgat aaacgatgaa cccacggtat aaagtggatg ccttatccag cggtagatgc
     2281 cactggttgc aagtttatgt tctttacgcg ttgcactgac tggtgtaatt ccgctgccaa
     2341 tactggtgaa caaccaataa attccgaaga cacaaaatat accgaacacg actccgaacc
     2401 aacggctcca tgcgggtaaa ctgatcttcg accagttcat ccatacaggg ttgactaagt
     2461 agacgagcgg actgagccag agcaccaacc caaagatgcg
//
LOCUS       DAOQNJ010000423         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_32491_length_2498_cov_9.363078, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000423 DAOQNJ010000000
VERSION     DAOQNJ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32491_length_2498_cov_9.363078"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLG52_11070"
     CDS             complement(<1..380)
                     /locus_tag="PLG52_11070"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNB42033.1"
                     /translation="MPLEHAILAILDFQPMSGYDLKKFFDVSVAHFWSATQSHIYKSL
                     EGLEKKGWAEAQVIPQEGKPNRKEYQITAEGRSELRRWLVTPLPMDPVREAVLIQIFF
                     SHSSSNEEIAALFESRIKEIREQL"
     gene            559..2250
                     /locus_tag="PLG52_11075"
     CDS             559..2250
                     /locus_tag="PLG52_11075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNB42034.1"
                     /translation="MNRSEILAQLKNNPEVSVLIVGAGINGIGTFRDLALNGVDVLLI
                     DRGDFCSGASSASSHMAHGGIRYLENGEFRLVREAVKERNRMIQNAPHIVKPLPTTIP
                     MFKYFSGILNAPLKFMGWLDKPSERGSIIIKLGLMMYDAYTGSQRTVPRHKFYSRTES
                     LTKWNKLNPEIVNTAVYYDGLISNPERLALEIMLDAETENSNARALNYVSLVSGSGKN
                     VILRDELTDATVEIQPKLVINAAGPWIDFANHSLGVTTKFIGGTKGSHLVLDHPELRK
                     AIGDNEFFFENKDGRIVLIFPLQDRVLIGTSDIKIDHPDDARCTDEEVDYFLELTARV
                     FPNIKVTREHVVFAFSGVRPLASSMAKTTGQYSRDHHIEVLSGDWTNLSFPVYSLVGG
                     KWTSFRAFSEEVADKSLQFLGIKRNKGTQELPVGGGRNYPRTSEERTKYINGLAAWTG
                     LSHERLETLFERYGTRAESIAEFMKVATDAPLNSLPSYSKRELLFLIQYEKAEHLDDL
                     LLRRTMLAMLGKLTRDAVIECSMLMADALNWNLERRNAEAERTLRVLADRHRVRL"
     gene            2314..>2498
                     /locus_tag="PLG52_11080"
     CDS             2314..>2498
                     /locus_tag="PLG52_11080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB42035.1"
                     /translation="MSPRQKSKLQIDLVIPVYNEAEAIRQTYADIRKVVDALPYDFRF
                     IYVDDGSSDGTADTLRG"
BASE COUNT          643 a          571 c          598 g          686 t
ORIGIN      
        1 tgcaactgtt cacgaatttc ttttatccta gattcgaata gtgctgcgat ctcttcatta
       61 ctactagaat gcgaaaagaa aatttggata aggactgcct cgcgcacagg atccataggt
      121 aggggagtca ccagccagcg acgtaattcg cttcttcctt ccgccgtgat ctgatactct
      181 tttctgttgg gtttcccttc ctgtggaatg acctgtgcct ctgcccaacc ctttttttcc
      241 aatccttcaa gagatttata aatatggctc tgtgtcgcag accaaaagtg agcgacagaa
      301 acatcaaaga atttttttaa atcatagcca gacatgggct ggaaatcgag tatggcaaga
      361 atggcgtgtt ctaagggcat aatactcctc tagttttata tatctatata gatataatat
      421 caaatagtct tataagtgtc aaggttaacg aacccaatgt gttttcagtt ggatgctctg
      481 tttttgacgt ttgatcattc agcccaaaaa attagaaatc atcgtacaat taacccagca
      541 ttgtgaaggg atgttcaaat gaatcgttca gaaattctag ctcagctcaa aaataatcct
      601 gaagtttctg tcttgatcgt aggggcaggg attaacggga ttgggacttt tcgtgattta
      661 gcattgaatg gggttgacgt attgcttatt gatagagggg atttctgttc tggagcaagt
      721 tctgcttctt cacacatggc acacggtggg attcgttatc ttgagaatgg tgagtttcgg
      781 ttggtgcgtg aggcggttaa agaacgcaat cgtatgattc agaatgcgcc gcatatcgtc
      841 aaaccgttgc caacaaccat cccaatgttt aagtatttct ctggcatctt gaatgcacca
      901 ttaaagttta tgggctggct cgataaacct tctgaacgcg gctcgatcat tatcaaacta
      961 gggttgatga tgtacgatgc ctatacaggc agtcagcgta cagtgccgcg ccataaattc
     1021 tattcccgca ccgaatccct taccaaatgg aataagttaa atcctgagat cgtcaacacg
     1081 gcggtctatt atgacggatt gatttctaac ccagaacgcc tcgcacttga gatcatgctg
     1141 gatgctgaaa cagaaaattc aaatgcccgc gctttgaatt acgttagcct tgtgagcgga
     1201 tcgggcaaga acgtcatcct ccgtgatgag ttgaccgatg caaccgttga aatccagccg
     1261 aagttggtca ttaatgccgc cggtccatgg atcgactttg ccaaccacag cctgggtgtg
     1321 actacgaaat ttattggcgg cacgaaaggt tcacaccttg ttctcgatca tcccgaattg
     1381 cgaaaagcga tcggcgataa cgaatttttc tttgaaaata aagatgggcg tatcgtgttg
     1441 atcttcccgt tacaagaccg cgttttgatc ggcacctctg atatcaaaat cgatcatcca
     1501 gacgatgctc gttgcaccga tgaagaagtg gactactttc ttgaactaac cgcacgtgtt
     1561 ttcccgaata tcaaggtgac acgtgaacat gttgtttttg cattttcggg cgtgcgtccg
     1621 ctggcaagta gcatggcaaa aaccactggt caatacagcc gtgaccacca tattgaagtt
     1681 ctcagtgggg attggactaa cctgagcttc ccggtctatt cactggtggg tggcaagtgg
     1741 acctccttcc gtgctttctc tgaggaagtg gcagataagt ctttgcagtt cctcggtatt
     1801 aagcgcaata aaggcacaca ggaacttcca gttggcggcg ggcgcaatta tccgcgcacg
     1861 agcgaagagc gcaccaaata catcaacggt ctcgccgcgt ggacagggct ttcgcatgaa
     1921 cgccttgaaa cccttttcga acgttacggc acgcgcgccg aatctattgc tgagtttatg
     1981 aaagtggcaa ctgatgcccc tcttaattcg ttgccgagtt attccaaacg cgaattgctc
     2041 ttcttgattc agtatgaaaa ggcagagcat cttgatgatc tacttctgcg tcgcaccatg
     2101 cttgccatgt tgggcaaact cacacgcgat gcagtgatcg aatgctccat gttgatggca
     2161 gatgccctga attggaacct cgaacgtaga aatgccgagg cggagagaac gctccgtgtc
     2221 ttggcagacc ggcaccgggt ccggttatga agcgatataa acgaacgccg ccgttatcgg
     2281 cggtgttttt gtctgcgtgg tagaatcttc ctgatgtctc cacgtcaaaa atccaaactc
     2341 cagatcgacc ttgtcattcc tgtttataac gaagctgaag ccattcggca aacctatgcg
     2401 gacattcgca aagtggtgga tgcgctgccg tacgattttc gattcatcta tgtggatgac
     2461 ggctcaagcg acgggacagc ggatacgctt cgagggat
//
LOCUS       DAOQNJ010000424         2494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_32569_length_2494_cov_8.149651, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000424 DAOQNJ010000000
VERSION     DAOQNJ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32569_length_2494_cov_8.149651"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1027
                     /locus_tag="PLG52_11085"
     CDS             125..1027
                     /locus_tag="PLG52_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42036.1"
                     /translation="MQSFSRGWSFLKQAWSMAFKDKDLLKPSIYALIVGAIVSVIGII
                     PIAIAAFAFGNSDIGNIIIYAMGALLVFVQFIVTYMFSAMTVYLIYGYLSEGDGRMDK
                     AWEIVRRDFFDILTLAAASTVVNLIKNAAQRNRRGGVGASIARSATGLLETLWTEAAA
                     LVLPAMVIDDLNLKDGMQRIWKITKENFLLVGISTIGVRFVTGLIGFVFSMIGLILAF
                     AIGGGLAFASGGDTTLSIIGVVVGALIFFAFVMISSVFSSYTTTAYHTCLYIWAREVE
                     KATAQGMAPAQVRAPAPLAAVLAS"
     gene            1090..1605
                     /locus_tag="PLG52_11090"
     CDS             1090..1605
                     /locus_tag="PLG52_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNB42037.1"
                     /translation="MKPEPRRQEAITWEEVDKLIDHLIPQFRREFTAMVMITRGGIIL
                     GGMLAEAMDITHILTAAVDFPAQTQSQQSSAFMAWPEFIQFPADDKLRGRPTLIVDDV
                     WGSGRTITAVKNRVSAAGGFPETCVLHFNPYRNLFGSVRPDYYAAVTDAFITYPWEIN
                     RGPDQVLLGEV"
     gene            1611..2294
                     /locus_tag="PLG52_11095"
     CDS             1611..2294
                     /locus_tag="PLG52_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB42038.1"
                     /translation="MKTIESTLKQKMDALRGNQYFDDVSDEILKDIATHTRLGEFQRG
                     DVLFWEGDPCEGLFILQQGSAKIFRLSPQGRQYIVRILQEGDTFAEVPAFDEGTSPVN
                     VEALETCRTWIIDKNKLHTLVMEHPAFAQKVLVNFGRMLRGMVRMVSEMAFYQVTHRL
                     ARLIDTELPQDKSAAWTQEQLAARLGTVREVVARSIKELERSGAIKIEDRRIQIADRE
                     IFEQWIMPS"
BASE COUNT          600 a          579 c          635 g          680 t
ORIGIN      
        1 tggcatatcc tgaggttgga attgcccgat tatacatgga caccctaaaa aggaagcaag
       61 cagtttggta agtttgctat aatttgtttg tccaaacttc ggttcgttta taggagagtg
      121 tagcatgcaa agtttttcac gtggttggtc atttttaaag caagcctgga gcatggcatt
      181 caaagataag gatcttctca aaccgtctat ctatgctttg atcgtcggtg ctattgtttc
      241 tgtcatcgga attattccta ttgcgattgc tgcttttgca tttgggaaca gcgatatcgg
      301 gaacatcatc atttatgcca tgggtgcctt attggtcttc gttcaattta ttgtgactta
      361 tatgttctca gccatgacgg tgtatcttat ttatgggtat ctttccgaag gcgatggtcg
      421 catggacaaa gcgtgggaaa ttgtccgccg tgactttttc gatatcctta ccttggctgc
      481 tgcttctaca gttgtgaact tgatcaaaaa cgcggctcaa cgcaatcgtc gcggtggggt
      541 tggcgccagc attgcccggt ctgcaacggg tttgttggaa accttgtgga cggaagccgc
      601 cgctctcgtt ttgcccgcta tggtgatcga tgatctcaat ctcaaagatg gaatgcaacg
      661 tatttggaaa atcaccaaag agaacttcct attggttggt attagcacca ttggtgtgcg
      721 cttcgttacc ggactgattg ggtttgtttt cagcatgatc ggcttgattc tcgcttttgc
      781 catcggtggc ggacttgcct tcgcttctgg tggagatacc actctttcca ttataggtgt
      841 tgtggttggg gcgttgatct tctttgcctt tgtgatgatt tcaagtgtat tcagctcgta
      901 taccacgacg gcatatcaca cctgccttta tatctgggca cgtgaagtgg aaaaagccac
      961 ggcgcaagga atggctccgg cacaggtacg ggctccggct ccgctcgcgg cggttcttgc
     1021 atcctaagaa aaatatgatg agaccctaaa ggtcagcatc ctttagggtc ttttgataaa
     1081 atatctacta tgaaacctga accccgccga caagaagcca tcacttggga agaagtggac
     1141 aagctgattg accacctcat tccacaattt cgccgtgaat ttaccgccat ggtgatgatc
     1201 acgcgcggcg ggattatcct tggcggcatg ttagcagaag ccatggatat tacccacatc
     1261 ctgactgctg cggtggattt ccctgcccag actcagagcc agcaatcttc tgcattcatg
     1321 gcgtggcccg agtttattca gtttcctgcg gatgataaat tacgtggtcg cccaaccttg
     1381 atcgtggatg atgtgtgggg ttctgggcgt acgatcacgg ctgtaaagaa tcgcgtttca
     1441 gcggcaggcg ggttccctga aacttgtgtg ttgcatttca acccataccg caatttgttt
     1501 ggcagtgtgc gccccgatta ttacgctgct gtcacggatg ctttcatcac ctatccttgg
     1561 gaaatcaatc gcggacccga tcaggtgcta ttgggcgaag tgtaattgga atgaaaacga
     1621 tcgaatccac cttaaaacaa aagatggacg ccttgcgggg aaatcagtat tttgacgatg
     1681 tatcagatga aatcttgaaa gacatagcaa cccatacccg ccttggagaa ttccagcgcg
     1741 gggatgttct tttttgggaa ggtgatccct gcgaaggttt gttcatccta caacagggca
     1801 gcgccaagat atttcgcctc tcgccacagg gacggcaata catcgtccgc attcttcaag
     1861 agggcgacac cttcgcagaa gttcctgcct tcgatgaagg caccagcccg gtcaatgtag
     1921 aggcgctgga aacttgccgc acttggatca tcgataagaa caaactacat acactggtca
     1981 tggaacatcc tgcttttgcc caaaaggtct tggtcaactt tggtaggatg ctgcgcggca
     2041 tggtgcgcat ggtcagcgag atggcgttct atcaagtcac ccatcggctg gcgcgcttga
     2101 tcgacactga actgccacag gataaatccg ctgcctggac gcaggaacaa ctcgcggcgc
     2161 ggttgggaac cgtgcgtgaa gtcgtggcga gatcgatcaa ggaattggaa cgcagcggcg
     2221 cgatcaagat cgaagaccgt cgcattcaga tcgcagatag agaaatcttt gagcagtgga
     2281 tcatgccaag ttaaaataaa aagcggcgaa ttcgccgctt tttattttat ggaagaactt
     2341 caaagttctc cacctgataa aagatctcat catccaccgc ttcttttata tggtcaatgg
     2401 aaatcgaggt ggggaaacca tacatcgtat catacgtgac gagcacttcg tccgcatcgc
     2461 cattcaagct ggaatccaat gtagcaaaaa tagc
//
LOCUS       DAOQNJ010000425         2483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_32813_length_2483_cov_7.941516, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000425 DAOQNJ010000000
VERSION     DAOQNJ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32813_length_2483_cov_7.941516"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLG52_11100"
     CDS             complement(<1..191)
                     /locus_tag="PLG52_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42039.1"
                     /translation="MSSTIALLMFIALIGFIAFLFWRQNRIFNDIQAFYKDNKMVYQE
                     TAPVEHPFIYTSIKPICSKG"
     gene            complement(215..547)
                     /locus_tag="PLG52_11105"
     CDS             complement(215..547)
                     /locus_tag="PLG52_11105"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNB42040.1"
                     /translation="MAYDLKLAERIRAEVKGLPIVEKKMFGGVGYMLHGNMACGILED
                     NLIIRVALDDYEKTLKRKYVKIFTMKNGPRPMKGWVMVEPEGYKTSKQLKDWVKTGID
                     VAINLPPK"
     gene            complement(567..1193)
                     /gene="upp"
                     /locus_tag="PLG52_11110"
     CDS             complement(567..1193)
                     /gene="upp"
                     /locus_tag="PLG52_11110"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNB42041.1"
                     /translation="MSNVYASKHPLIAHKLSILRDKNTKPKKFRQLVKEIAGLLAYEA
                     TLDLATTPVEIETPLETTTAQELKEKIGLVPILRAGLGMVEGIWELMPSAEVWHIGLY
                     RDEKTLRPVEYYNKLPLEPRVAVCLILDPMLATGGSATATAEVLKRWGVTKIKFVGLI
                     AAPEGIKAMQTAHPDIDIYVAAIDDHLNERAYIVPGLGDAGDRQFGTG"
     gene            1296..1712
                     /locus_tag="PLG52_11115"
     CDS             1296..1712
                     /locus_tag="PLG52_11115"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HNB42042.1"
                     /translation="MNGNDFMAWILRSPFHGMLSNGMMMISITGRKTGKKITTPVGYY
                     MEGEYLWVITSRERKWWRNLQGGATVDLVLRRKPVQGFAELELDEKAVEARMSEYLRH
                     IPQAASRMGVRMVDGKPNQEDVARTAKDRLFVRIKL"
     gene            complement(1760..2302)
                     /locus_tag="PLG52_11120"
     CDS             complement(1760..2302)
                     /locus_tag="PLG52_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42043.1"
                     /translation="MSEFTMKLIKCPSCGGPIDPPSGESTAKCPYCGSAVVIPESLRK
                     PAVSNQNQSSLTSSANVFGVDLNSMMGYGAQWSQVVQLAQAGQKEEAVKKYMSFSSIS
                     EMDARRTVEGLANTQVYDVSAYTTSVANAYAPMVQSYTDTAKKITAWSFGFSCLITGF
                     IMLTVLLILGATFWAVFKSF"
     gene            2301..>2483
                     /locus_tag="PLG52_11125"
     CDS             2301..>2483
                     /locus_tag="PLG52_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42044.1"
                     /translation="MALLLWFAMVKLYEHQIFSIGAIRTSQLLALSAGWLGVRIASFQ
                     LDEEMFMTEQFTFGGEI"
BASE COUNT          610 a          540 c          628 g          705 t
ORIGIN      
        1 ccctttgaac agatcggttt tatgctggtg taaataaaag gatgctctac aggggcagtt
       61 tcttggtaga ccatcttatt gtctttgtaa aaagcttgaa tgtcgttgaa gattctattt
      121 tgacgccaaa acaagaaggc gataaaaccg atgagcgcaa taaacatcaa taaagcgatt
      181 gtggaagaca ttctcaatct ccttttatgt atatttactt gggaggtagg ttaatagcaa
      241 catctatgcc tgtttttacc caatccttaa gttgttttga tgttttgtat ccttctggct
      301 ccaccatgac ccagcccttc atgggtctgg gtccgttttt catggtgaat attttgacgt
      361 atttgcgttt gagagtcttt tcataatcat ccagcgcaac acgtatgatt aagttatctt
      421 caagaatgcc gcatgccatg ttgccatgca gcatatagcc aacgccgcca aacatctttt
      481 tctcgacgat gggtaagcct tttacttcag cgcgaatccg ctctgcaagt ttcaggtcgt
      541 atgccataag tgacttggtg tatcgtttac cctgttccga actgacggtc tccagcgtca
      601 cccaagccgg gtacgatgta cgcccgttcg ttcagatgat catcaatggc ggcaacatag
      661 atatcaatat cggggtgagc cgtttgcatg gctttaatgc cttctggcgc cgcgatcagc
      721 cctacgaatt tgatcttggt cactccccaa cgtttgagca cttccgctgt ggctgtggca
      781 gaaccaccgg tggcaagcat ggggtcaaga atgaggcaga ccgctacgcg cggctcaagc
      841 gggagtttgt tgtagtattc cacaggacgt agggtttttt catcacggta taaaccgata
      901 tgccaaactt cggcactggg catgagttcc caaatgcctt ccaccattcc cagcccggca
      961 cgtagaatcg gcacaagccc gatcttttct ttgagctctt gcgctgtagt agtttccagc
     1021 ggagtttcga tctcaactgg ggtggtggcg aggtcgagtg tggcttcata ggcaagaagt
     1081 cccgcgatct ctttcacgag ttggcggaac ttcttgggtt tggtgttctt atctcttagg
     1141 atggaaagtt tgtgggcaat taacggatgt ttggatgcgt aaacgttgga catgacatat
     1201 tctccaaaat gagttgacca attatagccg tgaaggtgat gtttgtctcg tgactcggct
     1261 ggttactttt gttacacttt tcaaaaggag ataacatgaa cggaaacgat tttatggcat
     1321 ggattttacg ctccccattt catggcatgt taagcaatgg catgatgatg attagcatca
     1381 ctgggcgaaa gactggcaag aaaatcacta cccctgttgg ttattatatg gagggtgagt
     1441 atttgtgggt gattaccagc cgcgagcgga agtggtggcg gaatttgcag ggcggagcaa
     1501 ccgtcgactt ggtgttgagg cgcaaaccgg ttcagggatt tgccgaactg gaattggatg
     1561 aaaaggctgt tgaggcgcgc atgtctgaat atctgcgtca tattccgcag gcagccagca
     1621 gaatgggcgt ccgcatggtg gatggaaagc caaatcagga agatgttgct cgaacagcaa
     1681 aagaccggct ttttgtaaga attaagttgt aacaagaaag aagcggatgg ttgaaattcc
     1741 atccgcttct tttataaggt tagaaagatt tgaatactgc ccaaaatgta gccccaagga
     1801 tcagtagaac ggtcagcatg ataaaccctg tgatcaaaca gctaaaacca aacgaccaag
     1861 cggtgatctt tttggcggtg tcagtatagc tttgaaccat aggagcatag gcattggcaa
     1921 cgctcgtggt ataggcgcta acatcatata cctgtgtatt tgccagccct tccacagtgc
     1981 ggcgagcatc catttcactg atgctggaga agctcatata ttttttaacg gcttcttctt
     2041 tttgtccagc ttgagcaagc tggacaactt gagaccactg cgccccatag cccatcatgg
     2101 aatttaagtc cacgccgaac acatttgcag atgacgtgag agacgattgg ttttggtttg
     2161 agactgctgg ttttctcaaa ctttctggga tgacaacggc actgccgcag taggggcatt
     2221 tggcggtgct ttctccgctg ggcgggtcaa tgggacctcc gcaactgggg cacttaatta
     2281 gcttcatcgt aaattcagac atggcacttc tcctctggtt tgcaatggtt aaattatatg
     2341 aacaccagat ttttagcata ggtgccattc gtactagtca acttcttgca ttgtccgctg
     2401 gctggcttgg ggttagaatc gcttcattcc aacttgacga ggagatgttc atgaccgaac
     2461 agtttacgtt tggcggtgag atc
//
LOCUS       DAOQNJ010000426         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_33058_length_2472_cov_7.395118, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000426 DAOQNJ010000000
VERSION     DAOQNJ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33058_length_2472_cov_7.395118"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLG52_11130"
     CDS             <1..511
                     /locus_tag="PLG52_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42045.1"
                     /translation="PSLSLELALAEVIDAPAEIAQVAVAPRTKPQPVSAPVAESKPEV
                     KSEAQPVVESPAINSNDIVKAWKPLINTLPKDKNTLGALMNSVRNIDVQGKTLILGMA
                     SDVLVEKLNKPDQIETIQRLIKDHFHVDLSVRCVVTNVKGKLPAHVAQDGMVAAAIQH
                     GGEIVDMDE"
     gene            553..996
                     /locus_tag="PLG52_11135"
     CDS             553..996
                     /locus_tag="PLG52_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42046.1"
                     /translation="MATSNKNNSGKKKDVKIEFTISPVLQQVEKKISEAQNFQLEVIA
                     RTNFNSFNGKRIAEWLRENHRMWRSVLLPLDFISLRDMTDGHWHADTLYIYPEDGYGF
                     KLEEIMKEQFHADETQWIGRESAMDMLGTSKIEDKSYVILSAWWD"
     gene            1049..1378
                     /locus_tag="PLG52_11140"
     CDS             1049..1378
                     /locus_tag="PLG52_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNB42047.1"
                     /translation="MAKGFNKGPAMGGQAGMMQQLQRMQKQMEEAQAKLAEETVSATA
                     GGGAIKVVMTGDQKCKSVEISPEFLKDADAEMLQDMVLSAVNMALDQSRDLQQKLMGP
                     LAGGLPF"
     gene            1449..2054
                     /gene="recR"
                     /locus_tag="PLG52_11145"
     CDS             1449..2054
                     /gene="recR"
                     /locus_tag="PLG52_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNB42048.1"
                     /translation="MLLPESIQNLVTALERLPGIGPKSASRLAFFLLRASEDVAQDLS
                     TALANLKANTAFCSECFNITDAGRERCEICESQKRDASLICVVEEALDVLALERTGGF
                     QGKYHVLQGVLSPIEGIGPDDLKIKQLAERVARGGVGEVIIATNPSMEGDATALYLRQ
                     QLEPMGVKVTRLARGLPVGGDLEYADQNTLLRALTGRHEMN"
     gene            2097..>2472
                     /locus_tag="PLG52_11150"
     CDS             2097..>2472
                     /locus_tag="PLG52_11150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB42049.1"
                     /translation="MNITILTFGSRGDVQPFLPLSLGLMSRGHKVILAAPARFKSLVE
                     EHGITFFPLAGDPEELSRRLNEAGYNFFKLINELMSHAVDIGAEMMRQTDEACRDADL
                     IVHTFAHAVGAHTLAREKNIPDI"
BASE COUNT          655 a          625 c          612 g          580 t
ORIGIN      
        1 gccctctttg agtctggaac tcgccctcgc ggaagtgatc gacgctccag ccgaaatcgc
       61 ccaagtcgcc gttgcgcctc gaaccaagcc tcagcctgta tccgctcccg tagcagaatc
      121 caagcctgag gttaagagtg aggcgcagcc tgtcgtcgaa tcgcccgcta ttaattccaa
      181 cgatatcgtc aaggcatgga agccgctgat caacacgctt cccaaagata aaaatacact
      241 tggcgcgttg atgaactctg tgcggaatat cgacgtgcaa ggcaagacgc tcatcctcgg
      301 catggcaagt gacgtgctcg tggagaagct caacaaaccc gaccagatcg aaaccatcca
      361 acgcctcatc aaagaccact tccacgtgga tttgtccgtg cgctgtgtcg tcaccaacgt
      421 taaaggtaaa ctccccgcac acgtcgcgca agatggcatg gtcgccgccg cgatccaaca
      481 tggtggcgag atcgtggata tggatgagta gagcggtaat tggagattag taattggtaa
      541 ttgaggctaa ttatggcgac ttcgaataaa aataacagcg gcaagaaaaa agatgtaaaa
      601 attgaattta cgatcagccc tgtgcttcag caagtagaga agaaaataag tgaggcacaa
      661 aattttcaat tagaagtcat tgcccgaaca aacttcaatt cgttcaatgg caaaagaatc
      721 gctgagtggc taagagaaaa tcaccgaatg tggcggtcag ttttgttgcc gctggatttt
      781 atttctctcc gtgatatgac agatgggcac tggcatgcag acacacttta tatttaccct
      841 gaagatggct atggctttaa attagaagaa attatgaaag aacaatttca cgccgatgaa
      901 acccaatgga ttgggcgaga gagtgcaatg gatatgcttg gcacaagtaa aatcgaagat
      961 aaatcgtatg tgattctttc cgcttggtgg gactgaatct ccaatatcca atctctaatt
     1021 accaattact taactaggag actattttat ggcaaaagga ttcaacaaag gacccgccat
     1081 gggcggacag gctggcatga tgcagcaatt gcaacgcatg caaaagcaaa tggaagaagc
     1141 gcaggcgaaa cttgccgaag aaaccgtatc cgcaactgcg ggtgggggag cgatcaaagt
     1201 cgtcatgact ggtgaccaga aatgcaaatc tgtcgaaatc tcgcccgaat ttctcaaaga
     1261 cgcagatgcc gaaatgctgc aagacatggt cttgtctgct gtcaatatgg cgctcgacca
     1321 atcccgcgac cttcagcaaa agttaatggg tccgctggcg ggtgggcttc ccttttagta
     1381 actggtaatt ggtaattgga gatttgtttg cacacccaat taccaattac taatctccaa
     1441 ttaccctcat gttactccct gagtcaattc aaaatttggt caccgccctc gaacgcctgc
     1501 ctggcatcgg acccaagtcc gcttcacggc tggcattctt ccttttgcgc gcctccgaag
     1561 atgttgcgca agatctgtcc acggcgttgg cgaatctcaa agccaatacc gccttctgct
     1621 ctgaatgttt caacatcacc gatgcagggc gtgaacgctg tgaaatttgc gaatcccaaa
     1681 aacgtgacgc atccctcatt tgcgttgttg aagaagccct ggatgtcctc gcgctggaac
     1741 gcaccggcgg ctttcaaggt aagtatcacg tcttgcaggg agttttatct cccatcgaag
     1801 gcatcggtcc cgacgatttg aagatcaagc aacttgctga acgtgtggca cgtgggggag
     1861 tgggggaggt tatcatcgcg accaatccca gcatggaagg ggatgcgacg gcgctttatc
     1921 tccgtcaaca acttgagccg atgggcgtaa aagtcacccg ccttgcgcgc ggtctgcccg
     1981 tgggtggcga cctggaatat gccgatcaaa ataccctgct gcgtgcgctt acaggcaggc
     2041 atgaaatgaa ctaatccctg atggggcgcg agtaccgcgc ccctacgatt ttaaaaatga
     2101 acattactat tcttaccttc ggctctcgcg gcgatgtcca gccatttctc cctttatctc
     2161 tcggcttgat gtctcgtggg cataaagtca ttcttgccgc gccagcgcgt ttcaagtcat
     2221 tggtggaaga gcatggcata acatttttcc cactcgcggg tgatcccgaa gaactcagcc
     2281 gccgccttaa tgaggcagga tataacttct tcaaactgat caacgagttg atgagtcatg
     2341 cagtggatat tggcgcagag atgatgcgcc aaaccgacga agcctgtcgc gatgcagatc
     2401 ttattgtcca caccttcgct cacgctgttg gtgctcacac ccttgctcgc gagaaaaaca
     2461 tccccgatat tc
//
LOCUS       DAOQNJ010000427         2465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_33225_length_2465_cov_6.847303, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000427 DAOQNJ010000000
VERSION     DAOQNJ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33225_length_2465_cov_6.847303"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1399
                     /locus_tag="PLG52_11155"
     CDS             95..1399
                     /locus_tag="PLG52_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNB42050.1"
                     /translation="MKIRRILLLLFIAFAAIQPVFAQTRDPMAVVLTADGPIMPPMLE
                     YIKRGIETADQLNAEVVVIQLNTPGGSVTTMLEIIKEIRASDVPVVVYVAPKNAFAAS
                     AGAMITISGHVAAMAPETSIGASSPISSSGENLNSTAETKAKELIKASIRPFVTPRGE
                     KAVELADAMVDEAKAVTANEALDANLIDFVSDDLEDLLQSLNGFTVQMEDGPRKLNTE
                     DVDIQMLNISFIEQLLLMLTDPNISFLLLAIGVQAVLIELSSPGGWAAGFLGAVCLTL
                     AAYGLGVLTVNWFGGVFLIIAFVLFVLDIKAPTHGALTTAGVASFIIGALVLFNSPGT
                     PEFQRVSVPLVIGTGLVIGLIFFTIMLIALRAQHRPVQTGEESFVGKIGTAKMFEGEA
                     GQVQVESELWSAEKAAGSDEIRKGDKIEVVEVRGLRLIVKKK"
     gene            1513..>2465
                     /gene="lipA"
                     /locus_tag="PLG52_11160"
     CDS             1513..>2465
                     /gene="lipA"
                     /locus_tag="PLG52_11160"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNB42051.1"
                     /translation="MPATPTRDRVSPTTDARPLRRPEWIKVRAPSGENYERVHSLMRS
                     KELHTVCEEAMCPNLGECWGSGTATFLMLGDVCTRTCAFCDIKHGKPALLDWGEAERV
                     AQAVKSMDLKHAVITSVNRDERRDGGAPIFAMVIRRIREVLPGCSIEVLIPDFKGSIE
                     ALKIVMDARPEILNHNVETVPRLFKTIQPQDKYEWAAATLSNAKKLDPDVLTKSGIMV
                     GIGETMDEIKQVMRDQRSWGVDILTIGQYLQPSKKHMPMDRYYSMEEFAELREFGKEI
                     GFKWVESNPLVRSSYHAAEQVRALSVVHRKLYGNGAMSENQ"
BASE COUNT          613 a          636 c          636 g          580 t
ORIGIN      
        1 ggatttttgt ttgccatcca tgtaaatgtt tgcttagcat cgagtagaaa gctgaacaca
       61 aacagtataa tgatttaagg atttggagat actcatgaaa atcagacgca tcctcctttt
      121 attatttatt gcatttgcgg caattcaacc tgtctttgct caaacccgcg accctatggc
      181 tgtcgtcctc acagccgatg gtcccatcat gccgcccatg cttgagtaca taaaacgcgg
      241 catcgaaacc gcagaccagc tcaatgcaga agtggtcgtg attcaactca atacccccgg
      301 cggcagtgtc accaccatgc tggagatcat caaagaaatt cgcgccagtg acgtaccggt
      361 tgtggtgtat gtggcaccca aaaatgcctt cgccgccagc gccggagcga tgatcaccat
      421 ctcagggcat gtggcagcca tggcacccga aacatccatt ggggcatcca gccccatcag
      481 cagttcagga gaaaacctca acagcacggc ggaaaccaaa gccaaagaac tcatcaaagc
      541 ctccattcgt cccttcgtta ccccgcgcgg agaaaaagcg gttgaactcg ccgatgccat
      601 ggtcgatgaa gccaaagccg tcacagccaa tgaagcactg gatgcaaacc tgatcgattt
      661 cgtatccgat gacctcgaag acttgcttca atctctcaac ggtttcaccg tgcaaatgga
      721 ggatggtccg cgcaaattga ataccgaaga tgttgatatc caaatgctca acatcagttt
      781 catcgagcag ttattgctca tgcttaccga cccgaacatc tctttcttgt tgctcgccat
      841 cggtgtgcag gctgtgctca ttgaactttc cagcccgggt ggttgggcag ctggttttct
      901 tggggcggtc tgcctgacct tggcagccta cggcttgggc gtcttaacgg tcaactggtt
      961 tggtggtgtc ttcctcatca tcgccttcgt tttgttcgtg cttgatatca aagcgccaac
     1021 ccatggcgca ttaaccacag caggagttgc atcgttcatc attggcgcgc ttgtgttgtt
     1081 caactcgccg ggcacacctg aatttcaacg tgtatccgtt ccgctcgtta tcggcacagg
     1141 gcttgtgata gggctaatct ttttcacaat catgctcatt gctttaagag cgcaacaccg
     1201 cccggtccaa accggcgaag aatcattcgt cgggaaaata ggaacagcca aaatgttcga
     1261 aggcgaagcg gggcaagttc aagtggaatc tgagctttgg agcgcagaga aagcggcagg
     1321 gtccgatgag attcgtaagg gtgataaaat cgaagtcgtc gaagttcgtg gattaagatt
     1381 gattgtaaag aaaaagtaat atttcgtcat tgcgaggagc gtgtttttcg cgacgaagca
     1441 atctcatggc taaactggag attgcttcgg gctaccgccc tcgcaatgac gtaaccgcct
     1501 caaaaggacc ttatgcctgc taccccaacc cgagaccgag tctccccgac aacggatgca
     1561 cgccccctgc gccgccccga gtggatcaaa gtccgcgcgc cgtcaggtga aaattacgaa
     1621 cgtgtacatt cattgatgcg ttcgaaagaa ttacataccg tgtgtgaaga agccatgtgc
     1681 ccgaacctcg gcgaatgctg gggcagcgga actgccacct tcttaatgct tggcgatgtg
     1741 tgtacgcgta cttgcgcctt ctgtgacatt aaacacggca agcccgcttt attagattgg
     1801 ggcgaagccg aacgcgtggc gcaagccgtg aagtccatgg acttgaaaca cgcggtcatc
     1861 acctctgtga accgcgatga acgccgcgat ggtggtgcac ccatctttgc catggtcatt
     1921 cgccgcattc gtgaagtatt gccgggttgt tccattgaag tgttgattcc tgatttcaaa
     1981 ggcagcatcg aagccttgaa gatcgtgatg gatgcccgcc ctgaaatttt gaatcacaat
     2041 gtggaaaccg ttccgcgttt gttcaagacc attcaaccgc aagataaata tgaatgggct
     2101 gccgcaactc tttccaatgc caagaagctc gaccccgatg tgttgacgaa atcgggcatc
     2161 atggtcggca ttggcgagac gatggatgag atcaagcagg tgatgcgtga ccagcgctcc
     2221 tggggcgtgg acattctcac cattgggcag tatctgcaac ccagcaaaaa acacatgccg
     2281 atggatcgct actacagcat ggaagaattc gctgagttac gcgagttcgg caaagagatc
     2341 ggcttcaagt gggtcgagtc gaatccactg gtgcggtctt catatcatgc cgcagagcaa
     2401 gtgcgagcgc tgagcgtggt gcatcgcaag ttatacggca atggggcgat gagtgaaaat
     2461 caatt
//
LOCUS       DAOQNJ010000428         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_33313_length_2461_cov_8.360765, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000428 DAOQNJ010000000
VERSION     DAOQNJ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33313_length_2461_cov_8.360765"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="PLG52_11165"
     CDS             <1..1503
                     /locus_tag="PLG52_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNB42052.1"
                     /translation="LSGTFLILYATPQGMGLFDDSIAYIAGARSILAGQGYREAWLAS
                     NQPVTHFPPGFSTVLALVGISGLDPLRGTRLVNAVVFGANIFLLGLIGWRMTKSKLAG
                     MALSALLLLSGELFRVHTTAMSEPLYIFFTLAAFLAFSQTFVSEEKQTNKFLLLASAL
                     TAFAYLTRYAGLALFATFLVSLFLLHPTWKQRLTSAGIFLAGFVPFALAWAVRNRLLT
                     DNATNRKLVYHPITPENIQLGISNFASFILPIEEWRKVLIKVPNLVAVILIALILALL
                     GWVVLKGLKKFFNPASPTPEVLSFVSTLYVFGYLASILSSMTLFDASTKFQLRILAPV
                     YVSLLIMLVYFGYWLWQKQKIALILLSLVAFSFSVSSFLSTFTQLRKGGQGYASFQWF
                     DSEAMKFMQNLPAEARIYSNETAAVYLYTDHPGYVLPDLIDPVTGLPRGRFDEGVKAL
                     QEDVLSGNAVLALFDVDSQDEDVQSTYQILANGLYLAFDKQGDKIYTAYP"
     gene            1515..2369
                     /locus_tag="PLG52_11170"
     CDS             1515..2369
                     /locus_tag="PLG52_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB42053.1"
                     /translation="MSLTQKGIQMTIFDKFSLKDRVAVVTGGGGQLGFEFCKTLAEAG
                     AAVVAADLNMDFAGKTATRLTEAGYAAMAFPLDVTRPESTRELVAETVKQFGRLDILV
                     NSAALDPKFDPTAAEKGIAPGAFEDYPLADWQAALNVNLTGMFLTTQACVKQMVSQGK
                     KGSVINICSTYGLNGPDQRIYIKDGKRVAYKPVYYTTTKAGVMGFTKYLAAYYAETEI
                     RVNALTPGGVYNNHEEYFVKNYSAKTILGRMAKKDEMNGALLFLASDASSYMTGNNVI
                     VDGGWTAW"
BASE COUNT          542 a          634 c          564 g          721 t
ORIGIN      
        1 ctttcaggga cctttctcat cctgtatgcg actccacaag gcatgggact cttcgatgat
       61 tccattgctt atattgccgg tgcgagaagc atcttagcgg ggcaaggcta tcgcgaagcg
      121 tggcttgctt ctaatcaacc agttacacac ttccctccag gtttttcaac tgttttagcc
      181 ctcgtcggca tcagcggact tgaccctttg cgtggcacac gcttggtcaa tgctgttgtg
      241 tttggcgcaa atatcttttt gcttgggctg attggttggc gcatgaccaa gtcgaaattg
      301 gctgggatgg ctttgtctgc tttgcttttg ttaagcgggg aacttttccg cgttcatacc
      361 acggcaatga gcgagccgct gtatatcttt tttacactgg cggcatttct agccttctct
      421 caaacgtttg tctctgaaga gaaacagaca aacaaatttt tgttactggc gtctgctctt
      481 accgcttttg cttacctaac ccggtatgcc ggtcttgccc tctttgcgac ctttctcgtt
      541 tcactcttcc ttcttcatcc cacttggaag caacgcctaa ctagcgcagg tattttcctc
      601 gcaggctttg ttccttttgc gcttgcttgg gcggttcgta atcgcttact gaccgacaat
      661 gccaccaacc gcaagctggt ctatcacccg atcactcctg aaaatattca gcttggcatt
      721 tccaatttcg cctcttttat tttgcccatt gaagaatggc gtaaggttct gatcaaagtt
      781 ccaaatctgg tcgctgttat tcttattgct cttattttgg ctctgttggg ttgggttgtg
      841 ctcaaagggt tgaaaaaatt ttttaacccg gcttctccaa cccctgaagt tctttccttt
      901 gtcagcacgc tttatgtttt tggctatctt gcatccatcc tttcttcgat gacattgttc
      961 gatgccagca ccaaattcca actccgcatc cttgcgccag tgtacgtcag cctgctcatt
     1021 atgttggtct attttgggta ctggctttgg caaaaacaaa aaatagccct cattctcttg
     1081 tcactggtcg ccttctcttt ttctgtttct agttttttga gtaccttcac ccaactccgc
     1141 aaaggcgggc agggatatgc ttccttccag tggttcgact cagaagcgat gaagttcatg
     1201 caaaatcttc ctgcagaggc gcgtatctat tcgaatgaaa cggcggctgt atatctttat
     1261 acggaccacc ctgggtacgt cttgcccgat ctcattgacc cggtcaccgg tttgccgcgt
     1321 gggcgttttg acgaaggagt caaagcattg caagaagatg ttttatctgg aaatgccgtg
     1381 ctggcgctgt tcgatgttga ttcgcaggat gaggatgtac aatcaacgta tcaaattctc
     1441 gccaatggtt tgtatctcgc ttttgacaaa cagggcgata aaatctatac cgcatatccg
     1501 taaggtgaca tacgatgtcc cttacacaaa aaggaattca gatgactatt ttcgataaat
     1561 ttagtttgaa agaccgtgtg gctgttgtca ctgggggcgg tgggcaactt ggttttgagt
     1621 tttgcaagac ccttgctgaa gctggagctg ctgtggttgc ggcagacctc aatatggatt
     1681 ttgctgggaa aaccgcgacc cgcctcaccg aggctgggta cgccgccatg gcatttcccc
     1741 tcgatgtgac ccgccccgaa tcgacccgcg agttggtcgc tgaaaccgtc aagcagtttg
     1801 gtcgccttga tattctcgtc aactccgcag ctcttgaccc aaaatttgac cccactgccg
     1861 ctgaaaaggg catcgccccc ggcgcctttg aagattatcc acttgcagac tggcaagccg
     1921 ccctcaacgt caacctgact ggcatgttcc tcactaccca agcctgtgtg aaacagatgg
     1981 tgtcgcaagg taaaaagggc agcgttatca acatctgctc tacctacggc ttgaacggac
     2041 ctgaccaacg tatttacatc aaagacggca aacgggtagc ctataaaccc gtctattaca
     2101 ccaccaccaa agccggtgtg atgggtttta ccaagtacct tgccgcttac tatgctgaga
     2161 cggagatccg cgtcaatgca ttaacccccg gcggtgttta caacaaccac gaagaatatt
     2221 tcgtcaagaa ttattctgcc aaaactatcc ttggacgcat ggcaaagaaa gacgaaatga
     2281 atggtgcctt gctcttcctc gcttcggatg cctcttctta catgaccggc aacaacgtta
     2341 ttgtcgacgg tggttggacg gcgtggtagt gagcgatact cggttattga agatcaaaga
     2401 tcgaattgcg aatatcgaga acttatgacc aatattcttg ccattatccc tgctcgcggc
     2461 g
//
LOCUS       DAOQNJ010000429         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_33355_length_2459_cov_7.503744, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000429 DAOQNJ010000000
VERSION     DAOQNJ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33355_length_2459_cov_7.503744"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PLG52_11175"
     CDS             <1..1213
                     /locus_tag="PLG52_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNB42054.1"
                     /translation="YLLLSISNVEDDEPAALSNPAPYSILAGIFALGFAACALTSSWW
                     GLVRNADLLDRTDNARRAIADRYVPRGKILDNNNQPINVSTGESGSFIRTYLYPELAP
                     ITGYTHPVYGQAGLEANLDDYLRGLQGNPTSLILWNQLIYGTPPAGLDVRLSLDLNLQ
                     STADALLGKHRGAIILMNAESGEILVMASHPTYDPNKLDEEGDSLSQNDAAPLLNRAT
                     QGMYPVGDALLPLLKANFGEATVTDNELLSFYDQLGLFRSPLITLPVAESQPTEKVQD
                     LRASPLQVALATAALSNHGVIPAPRIATAVNTPEQGWVVLPVLDKPRELFSAEIADEA
                     AHAFVQQGNAYWKYIGQGVINKTTVTWLIAGTPPDWQGAPLAVVIAVEENNPQLVEQI
                     GASLFNSVLSQ"
     gene            complement(1259..1672)
                     /locus_tag="PLG52_11180"
     CDS             complement(1259..1672)
                     /locus_tag="PLG52_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNB42055.1"
                     /translation="MTHIITSLCVRDRGCIEVCPVECIVPGNPADGFPMVYIDPDTCI
                     DCGACVPECPFSAIFPEDEVPSSYTAKGTEYINRVGLSGHYEASNHHGKPVVLETTKK
                     LDAGETVDLTPDTQLNYDFFKSGPGYSAKDTDSGS"
     gene            complement(1793..2395)
                     /locus_tag="PLG52_11185"
     CDS             complement(1793..2395)
                     /locus_tag="PLG52_11185"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNB42056.1"
                     /translation="MAFELPKLAYAYDALEPHIDARTMEIHHTKHHQTYITNLNGAVE
                     KTPELQGKSLEALLSDLNAVPEGVRAVIRNHGGGTYNHNLFWEIMGPNAGGAPKGDLA
                     KAIDASFNSFDAFKDEFTKAATTRFGSGWAWLVKKGSGLAVVSTANQDTPLSDGLTPI
                     LGIDVWEHAYYLNYQNRRPDYITAFYNVINWEAVSAKFAA"
BASE COUNT          601 a          594 c          660 g          604 t
ORIGIN      
        1 gtatctgtta ctgtctatca gtaatgttga agatgatgaa cccgcagcgc tttcgaaccc
       61 ggctccatat tccatcctag caggcatctt cgcccttggc ttcgccgctt gtgctctcac
      121 aagctcatgg tggggactcg tacgaaatgc tgacttactt gaccgcactg ataacgcccg
      181 ccgcgccatt gcagaccgat acgttccgcg cggcaaaatt ttagataaca ataatcaacc
      241 cattaacgtt tctacgggtg aaagcggctc tttcattcgc acctatttgt accctgagct
      301 tgcgccaatc acgggatata cccaccccgt ctatgggcaa gctggtttgg aagccaacct
      361 tgatgattat ctacgcgggc tacaaggcaa cccaaccagc ctcatcctct ggaatcaact
      421 catttatggc acaccgccag ccggattgga tgtgcgttta agcctggacc tcaatttgca
      481 atctacggca gatgctttgt taggaaaaca tcgaggcgcc atcattctca tgaatgcaga
      541 gagcggcgag attctggtca tggcatcgca ccctacgtat gaccctaaca aacttgatga
      601 agagggagac tctctttcac agaacgatgc cgcgccatta ctgaatcgcg ccacccaagg
      661 tatgtaccca gttggagatg cacttctgcc gctcttaaag gcaaacttcg gcgaagcaac
      721 cgttaccgat aacgagttgc tctctttcta cgatcaactc ggactcttcc gttccccatt
      781 aattacctta cccgtggcag aaagccagcc tactgaaaaa gtgcaagacc tgcgagccag
      841 cccattacaa gttgcattag caacagcagc attgagcaac cacggagtga tccctgcgcc
      901 acgcattgca acggcggtaa acacgcccga acagggatgg gtggtgctgc cagtgttgga
      961 taagccgcga gagttgttct cagccgaaat tgcggatgag gcggcgcatg cttttgtcca
     1021 gcaagggaat gcgtattgga aatatatagg tcagggtgtg atcaacaaga cgacggtcac
     1081 atggctgatt gctggaacgc cgcccgattg gcaaggtgct ccgttggcag tggtgatcgc
     1141 ggtggaagaa aataatccgc aactcgttga acagatcggc gcaagtttat tcaattcggt
     1201 gttgagtcag tagaatcaaa aaagccaggc aggtgcctgg ctttttattt tcaatggact
     1261 agcttccgct atcggtgtct ttggcgctgt agccaggacc actcttgaag aagtcgtagt
     1321 tgagctgggt atcaggagtg agatcgacgg tttcaccagc atcgagcttc ttggtggttt
     1381 cgagaacaac cggcttgccg tggtgattgg aagcttcgta gtgaccagag agaccaacac
     1441 gattgatgta ctctgtgcct ttggcagtgt aagatgaagg aacttcatct tcagggaaga
     1501 tggcactgaa aggacattcg ggcacgcaag cgccgcagtc gatgcaggtg tcaggatcga
     1561 tatagaccat ggggaagcca tcagcaggat tgccggggac gatacattcg acggggcaaa
     1621 cttcgatgca tccgcggtca cgaacgcaca aactggtaat aatatgagtc atttcttttc
     1681 tccttgtggg atatagacaa acgatttctt cctattataa ctttgtttac gtgagtgaca
     1741 aggacaaaaa aacaccccgt attgctacgg ggtgtttttt acatcgtacg tactaagcag
     1801 caaattttgc agagacagct tcccaattga ttacattgta aaacgcggtg atgtaatcgg
     1861 ggcggcggtt ttgatagttc aagtagtaag cgtgttccca cacgtcgata ccgagaatgg
     1921 gagtgagacc gtcagaaagc ggagtgtctt gattggctgt ggagacaacg gcgagtccgc
     1981 tgcccttctt gacgagccaa gcccagccgg agccgaagcg agtggtggcg gctttggtga
     2041 attcatcctt gaatgcatca aaggagttaa aggaagcatc aatcgccttg gcgaggtcac
     2101 ctttgggggc gccgccggcg ttgggtccca taatttccca gaaaaggttg tgattgtagg
     2161 tgccgccgcc atgattgcgg ataacggcgc gaacaccttc aggaactgcg ttcaaatcgc
     2221 tgagcaaggc ttcgagggat ttgccctgaa gttcgggggt cttttcaaca gcgccattga
     2281 ggttggtgat ataggtctga tggtgcttgg tgtgatggat ctccatcgtg cgcgcatcaa
     2341 tgtggggttc aagcgcatcg tacgcgtatg caagtttggg aagttcaaaa gccataatag
     2401 ttctccttcg agttgattat ttgagttggt ctttccattg tatcccgccc gtgggctgg
//
LOCUS       DAOQNJ010000430         2458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_33384_length_2458_cov_10.726176, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000430 DAOQNJ010000000
VERSION     DAOQNJ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33384_length_2458_cov_10.726176"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="PLG52_11190"
     CDS             complement(<1..1783)
                     /locus_tag="PLG52_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42057.1"
                     /translation="MKKKNFPEILSLLLISAIVYLPHIGSLTYYKDDWYYIYDGMLGG
                     AKIFHEMFRIDRPARGFFFEWYYTLFGPNPLPWHLSVFVWRALAALGALWIFNLLWKE
                     NRKFNFIAALLFAIYPGYFWWISAIEYQPMIASLALQVFSIAFTLKAIQSAKIAEKAA
                     YGIGSILTGWSYIALVDYAIGMEAFRFIAVYLLIAHDLNNIFWKRLLVTLKVWAWTVL
                     IPLGIVIWRVFFFTNERKATDIGGQLSVFFSNPVSVGIDWLLQIYNSVLNLSVQAWIN
                     QFPTFIQSMRLRDITLSIVLAVIVTLLVVFAEKQIKQSAETKDEGGKQAKVTNEALIL
                     GGLGMIFGVLPVVMANRYVNISGFSHYGLPASLAAAVFMAGIIEIIALSSVRKAVLYG
                     VIVFASLSHFGIATNAATEEKALQEFWWQVSWRVPDIQPGTTLVIYYPLPGMGDGGAA
                     LMEAANVIYFPEFTGDAIVHYPLSGLTLNSENLPGILDGTQIVESGYRSHTYTFDYTN
                     ILVMSQPTLNSCVHVMNASALLISEYDPVDVSLVASQSKIENVIADAEPVSPPEYIYG
                     KEPDTGWCSYFQAADLAAQTGDWERVAA"
     gene            complement(1826..>2458)
                     /locus_tag="PLG52_11195"
     CDS             complement(1826..>2458)
                     /locus_tag="PLG52_11195"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB42058.1"
                     /translation="SGPVQYEEYKTYSAGYEKRVCLDISIQALREARTRIGDHGLFVV
                     GDLANLPFKKDAFDGAVSMHAIHHLALPEHPKAYAEIHRVLAQGRTAAIVNGWHNPLL
                     IRMAEPLIGLMRKLSGRSEKKKKEWLSEEDPAGTFVQKMTPEWLKKEIGSKMTIEIKP
                     WRSMSTRIMRWFVRPFGGRAFLRFVFWLEGVFPKFFAENGQYPLIVVRKS"
BASE COUNT          737 a          644 c          525 g          552 t
ORIGIN      
        1 gagcagcaac ccgctcccag tccccggttt gagccgcaag gtccgctgct tggaaataac
       61 tacaccagcc agtatccggt tctttgccat aaatatattc aggcggcgag accggttctg
      121 catctgctat tacattttcg atcttcgatt gtgaggcaac caatgaaaca tccactgggt
      181 cgtattcgga gatcaacagc gcacttgcat tcatcacatg tacacatgaa ttcaacgtag
      241 gctgactcat taccaatata ttggtgtaat caaaagtata agtatggctg cgatacccag
      301 actcaacaat ctgcgttcca tctaaaatac cgggcaggtt ttcgctattc aaggtgagcc
      361 ccgaaagcgg ataatgcaca atagcatcac ccgtgaactc agggaaataa atcacattcg
      421 cggcttccat caatgccgcg ccgccatcgc ccatgccggg caacggataa tagatcacaa
      481 gtgtcgtacc aggttgaata tccggcacac gccaactcac ctgccaccaa aattcctgta
      541 gcgctttttc ttccgtcgct gcattggtcg caattccaaa atgagataac gacgcaaaca
      601 cgatcacccc atataacact gctttgcgca cagatgacag cgcaataatc tcaataatgc
      661 cagccataaa aacagctgcc gccaaagatg caggcaaacc ataatgtgaa aaaccggaaa
      721 tgttgacata acgattcgcc atcacaaccg ggaggacacc aaaaatcatt cccagcccgc
      781 ccagaattag cgcctcattc gtgactttag cttgtttgcc accttcatct ttggtctccg
      841 ctgattgctt gatctgcttc tcggcaaaga ccacaagcaa agtcacgatc acagcaagca
      901 caatactcaa tgtgatatca cgcaaacgca tcgactgaat aaaagtgggg aactgattaa
      961 tccatgcttg gacgctgaga ttcaaaacac tgttatagat ttgaagcaac cagtcgatcc
     1021 cgacggagac tgggttagaa aagaaaacac ttaactgtcc gccaatatct gtggctttgc
     1081 gctcattggt gaaaaagaag acacgccaaa taactatgcc cagtggaata agaaccgtcc
     1141 acgcccaaac tttgagcgta acaagcaaac gcttccaaaa tatgttgttg aggtcatgtg
     1201 caatcaacag atatacagcg ataaagcgaa aggcttccat gccaatagcg tagtcaacaa
     1261 gagcaatgta actccaacca gtcaggatcg agccaatacc ataagcagct ttttcagcaa
     1321 tcttcgcaga ctgtatcgcc ttcaacgtga acgcaatcga aaagacctgt aatgccaaac
     1381 tcgcaatcat aggctggtat tcaatggcgc tgatccacca gaagtagcca gggtaaatcg
     1441 caaataaaag cgcggcaatg aagttaaatt ttcgattttc tttccaaaga aggttgaata
     1501 tccacaatgc gccaagggca gctaaagcac gccaaacaaa gacactcaaa tgccacggca
     1561 gcggattcgg tccaaagagg gtgtaatacc actcaaagaa aaatccgcgc gccgggcggt
     1621 cgatgcggaa catttcatgg aaaatcttcg ccccacccaa catgccatcg tagatgtaat
     1681 accagtcgtc tttgtaatag gtcaaactac caatgtgcgg caaatacaca atggcagaaa
     1741 taagcagaag tgagaggatt tcagggaagt tttttttctt catgtacgat tgccgattta
     1801 ctattttcga ctcacgtatt acgaattacg atttacgaac cacaatcaac ggatactgtc
     1861 cattttcggc gaagaacttc gggaagacac cttccaacca gaaaacaaac ctcaagaaag
     1921 cccgtccgcc aaaaggacgc acaaaccagc gcataatgcg ggtgctcata ctgcgccacg
     1981 gcttgatctc gatggtcatc tttgagccga tttctttctt cagccactct ggtgtcatct
     2041 tctgaacgaa cgtcccagcg gggtcctctt cgctcaacca ttccttcttt ttcttctcag
     2101 agcgcccaga cagtttgcgc atcaaaccga tgagcggttc tgccatgcga atcagcaacg
     2161 gattgtgcca accattgacg atggcagccg tgcgaccctg tgcaaggacg cggtgaattt
     2221 ccgcataagc tttggggtgt tcgggcaagg caagatgatg aatggcgtgc atagaaaccg
     2281 cgccatcaaa agcatccttt ttgaaaggca gattcgccaa atccccaacg acaaatagac
     2341 cgtggtcccc aatacgagtc cgtgcttcgc gcaaggcttg aatggaaata tccaagcaga
     2401 cgcgcttttc gtagcccgca gaataggtct tgtattcctc atactggaca ggaccaga
//
LOCUS       DAOQNJ010000431         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_33898_length_2438_cov_6.549308, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000431 DAOQNJ010000000
VERSION     DAOQNJ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33898_length_2438_cov_6.549308"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PLG52_11200"
     CDS             complement(<1..892)
                     /locus_tag="PLG52_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42059.1"
                     /translation="MLTLVEKILFAVAVVVSLYFTYKGVMKIVGHIGSGQGKVDWSLL
                     WKRIGDLIVKVGLFQPVFRLRLGPSIFHALIGWGFLSFLLINLADLIYAYTGWRLLEN
                     TGIFGDAYRLLADIANVAIIVGIIAMVIRRFILRPATLSTRETTLLNPKARTGILRDS
                     AIVATFIFVHNSMRFLGEAFGLALENKIDAWQPSISAVSKFFVGMSPTALTIGEHLAF
                     WLSIGAVVAFLPYFPISKHIHLFFAPLNFALKPERKSIGQLSYINLDDQSIEQFGAAK
                     MKDLGWEQIMDSFACIQCFRC"
     gene            complement(1000..>2438)
                     /locus_tag="PLG52_11205"
     CDS             complement(1000..>2438)
                     /locus_tag="PLG52_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42060.1"
                     /translation="LTIDHDGMPSPDEVYRDCGTIIYRNWMAQPMCNVNNVANCEGYY
                     AKLIDTDTKEKEIGMQLPAASAWISLEDCEPVYSTSTNICETKPTLVITGQEPLPNES
                     IVRVEGTYDEQTFFCDETDVCKFRIPETDDEGVKVEFWSYSSYGDSSVIYEAQVRVKQ
                     VDEGDPDQLYWYVDVLSPQWQGEVSATCAEIWGVFPPVGGAPEWLSTPPQSEDLSSDI
                     PYTYLAANLILQGVVDARSCPDGGLSPGNVANQCGLEKSRDAVNEWQNQFDGLILETA
                     KKTSVPAFLLKNLFARESQFWPGIFQAVGDIGLGQLTENGADTALFWNKSFYNQFCPL
                     VMDSETCGEGYANLEEEQQVQLRRALVGSVDASCEDCPLGLDLTQADFSVGVFANTLI
                     ANCEQTAQVIYNYTGSQTSKAASYEDLWKFTLVNYNAGGGCLAEAISGANGTSGLPLT
                     WETVSPNLIGTCASAVDYVNDISKETNP"
BASE COUNT          657 a          565 c          578 g          638 t
ORIGIN      
        1 gacaacggaa gcattggata caagcgaagc tatccatgat ctgttcccag ccgagatctt
       61 tcattttggc agcaccaaac tgttcaatcg attggtcatc aaggttgata tagctcaact
      121 gaccaatgga ttttcgttca ggcttgagtg caaaattcaa cggcgcaaag aatagatgaa
      181 tatgtttcga gatggggaag taaggcaaga aggcaactac agcaccaatc gacaaccaaa
      241 aagcgagatg ctcgccgatg gtcaatgctg tagggctcat gcctacgaaa aattttgaaa
      301 cagccgagat gctgggttgc catgcatcta ttttattctc aagtgcaagc ccaaatgcct
      361 cgcccaaaaa acgcattgag ttatggacga atatgaaggt ggcaacgatg gctgagtctc
      421 gtaagatgcc ggtgcgagct ttcgggttga gtaacgtcgt ctcacgtgtg gacaaagtcg
      481 ccggtcgaag aatgaagcga cgaatgacca ttgcgatgat gccgacaatg atggcaacat
      541 tagcaatatc tgccagcaga cgataggcat ccccaaagat gccagtgttt tcaagcaaac
      601 gccaaccggt gtaggcgtaa atcaaatctg cgaggttgat gagaaggaaa gagaggaagc
      661 cccagccgat gagggcatga aaaatgctgg gtccaagacg gaggcgaaaa acgggttgga
      721 acaagcctac tttgacgatc agatccccga tgcgtttcca caatagtgac cagtccactt
      781 tgccttgacc gctaccaatg tgacctacga tcttcatcac acctttgtag gtgaaatata
      841 atgaaacaac aactgcaact gcaaagagga ttttttcaac aagcgtgagc atggagcctc
      901 cgaaatgtgc tgcgaactca aagatgccac aaagtttagc acaactttat tcgattgtct
      961 caaaaatcac ttatgacgtt cttcctttta tcccccgaat tatggatttg tctccttgct
     1021 aatgtcgttg acataatcca ctgcgcttgc acatgttcca attaaatttg gtgagacggt
     1081 ttcccatgtg agaggcaatc cgcttgtgcc gtttgcgcca ctgatggctt cagccaaaca
     1141 tcctccgccc gcgttgtaat tgacaagggt gaacttccac aagtcttcat aggatgcggc
     1201 tttgctggtt tgagaacctg tgtagttgta aataacttgt gcagtttgtt cgcaattggc
     1261 gatcagtgtg tttgcaaaga caccgactga gaaatctgct tgtgtgagat ctaaaccgag
     1321 cggacaatct tcacaagacg catccacact gccaacgagc gcgcgtctta gctggacctg
     1381 ttgctcttcc tccaggttag catacccttc gccgcatgtt tctgaatcca tcaccaacgg
     1441 acagaattga ttgtagaaag atttattcca aaagagagcc gtatctgccc cgttctcggt
     1501 gagttgacca agaccaatat ccccaacggc ttggaagatg ccgggccaga attggctttc
     1561 tcgtgcaaaa agattcttta ataaaaatgc tgggacactt gtctttttgg ctgtttcaag
     1621 aattaaccca tcgaattgat tttgccattc attaactgca tcacgtgatt tttcaagccc
     1681 gcattgattg gcaacattgc cgggtgataa gccaccatcg gggcaggagc gcgcatcaac
     1741 cacgccttga agaataagat tcgccgcaag atatgtataa ggaatatcac tgcttaggtc
     1801 ttcgctttgc ggaggagtgc tgagccattc aggtgcaccg cctacgggag gaaagacacc
     1861 ccatatctcg gcgcaggttg cgctgacttc gccttgccat tgcggcgaaa gaacatctac
     1921 ataccagtag agttggtctg ggtcgccttc gtccacttgt ttgacgcgta cttgtgcttc
     1981 atagattacg ctactatccc cgtacgatga gtatgaccag aattcaactt ttacgccttc
     2041 atcgtctgtt tcagggatgc ggaatttaca tacatcagtc tcgtcacaaa agaaggtttg
     2101 ctcatcatac gtcccttcta cacggacgat ggattcattt ggaagaggtt cctgcccggt
     2161 aataacaagg gtaggtttgg tttcacaaat attggtggat gtactataga ctggttcaca
     2221 atcttcaaga gagatccatg cacttgcggc aggtagttgc atgccgatct ctttttcttt
     2281 tgtatcggta tcaataagtt ttgcgtaata cccctcacag tttgcgacat tattgacatt
     2341 gcacatgggt tgtgccatcc agttacgata gatgatcgtg ccgcagtctc tatatacttc
     2401 atctggagaa ggcatgccat cgtggtcgat ggtgagat
//
LOCUS       DAOQNJ010000432         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_33943_length_2436_cov_9.784964, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000432 DAOQNJ010000000
VERSION     DAOQNJ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33943_length_2436_cov_9.784964"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLG52_11210"
     CDS             complement(<1..646)
                     /locus_tag="PLG52_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNB42061.1"
                     /translation="MHLSETVNTGFYFLAPGLVLAPIIGLLINAIFGKNFSEKVVGTV
                     ASLASGAAFVISVLLAYSVAINHGHVEHVVFAEWIHIGDLELDWTFRVDSLSATMMLV
                     VSGVGTLIHIYAIGYMHEDVRFKHEEGRFARFFIFLNLFIAAMMILVSGDSYLMLFVG
                     WEGVGLCSFLLIGFWYEMDTLARPSWANSNAAKKAFITNRVGDFGFLMAGFLMFW"
     gene            complement(766..1068)
                     /gene="nuoK"
                     /locus_tag="PLG52_11215"
     CDS             complement(766..1068)
                     /gene="nuoK"
                     /locus_tag="PLG52_11215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNB42062.1"
                     /translation="MVPVDYYIVLSAILFTIGALGVLSRRNPLIIFMSIELMLNAANL
                     AFVAFARVYDTFSGQIFVFFVIAVAAAEVAVGLALIVEIFKSKKNINIDEMNSLKG"
     gene            complement(1068..1592)
                     /locus_tag="PLG52_11220"
     CDS             complement(1068..1592)
                     /locus_tag="PLG52_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNB42063.1"
                     /translation="MTSELIVFLVLSLVAIATALGMLFSRNAVYSALFLVLNFVTVAV
                     FYLLLGAPFIAMSQITVYAGAIMVLFLFVIMLLGAENLAVTNTLPWQRPLAATLSVIL
                     AVEAIYLLFVRARPEGVIASPDATLNSIDSLRNMALTLFNQFLLPFEVTSILLLVAMV
                     GAIVLTKQEKGAQS"
     gene            complement(1662..2183)
                     /gene="nuoI"
                     /locus_tag="PLG52_11225"
     CDS             complement(1662..2183)
                     /gene="nuoI"
                     /locus_tag="PLG52_11225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNB42064.1"
                     /translation="MGVFDPIFELAKGLGETFRSFLREPTVTYQYPEEKRPVRPRFRG
                     RHVLQRYDNGLEKCIGCSLCAAACPADAIFVEASENTDEKRFSPGERYASTYEINMLR
                     CIYCGFCEDACPTEAIVLGDNYELSFTDRRDAIYTKEMLLQPVSAENMVTPQKVETGV
                     FTRSVPEMKDPTD"
     gene            complement(2170..>2436)
                     /locus_tag="PLG52_11230"
     CDS             complement(2170..>2436)
                     /locus_tag="PLG52_11230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNB42065.1"
                     /translation="VIVILFGMIWIRATWPRIRYDRLMAFGWKVLLPLTLFLAFVTAV
                     GILLQQEFNNPLFFWSIPVVSIIGFFVAFAFVYRDLRRKSHGRV"
BASE COUNT          696 a          625 c          619 g          496 t
ORIGIN      
        1 gccagaacat caagaagcca gccatgagga agccaaagtc accgacacgg ttggtaatga
       61 aggctttttt cgcagcgttc gaatttgccc aggatggacg ggcaagggta tccatctcat
      121 accagaaacc aatgagcagg aaagagcaca agcccacgcc ttcccacccg acgaagagca
      181 ttaggtagga gtcgccagag acgaggatca tcatcgcagc gatgaacagg ttcaagaaaa
      241 tgaagaagcg ggcgaagcgt ccctcttcat gtttgaagcg cacatcctcg tgcatgtatc
      301 caatggcgta gatgtggatg agcgttccaa cgcccgaaac gaccaacatc atcgtcgccg
      361 aaagcgagtc gacgcggaat gtccaatcga gttcgaggtc gccgatgtgg atccattcgg
      421 cgaagactac atgttcaaca tgaccatgat tgattgccac cgaatatgcc aaaagcaccg
      481 agatgacgaa agccgctcct gatgcgaggc ttgccaccgt gcccaccact ttttcggaaa
      541 aattctttcc gaaaatggcg ttgatgagca aaccgattat cggtgccagc accaacccgg
      601 gggctagaaa ataaaaccct gtatttactg tttcacttaa gtgcataaca ttctccagag
      661 tttcccgtca ttgcgagggc gatgttcttt tgcccgaagc aatctcatga ttaagtctaa
      721 gattgcttcg tcgggaagag caccctcctc gcaatgacga agcctctatc cttttagaga
      781 attcatctca tcgatgttga tgttcttttt ggacttgaaa atttccacga tcaacgcgag
      841 acctactgca acctcggcag ccgccacagc aatgacgaag aagacaaaga tctgcccgct
      901 gaacgtatcg tacacgcgcg cgaaagccac gaaagccagg ttggccgcgt taagcatcaa
      961 ctcaatggac atgaaaataa tgagcgggtt gcggcgcgaa agtacaccca acgcaccgat
     1021 ggtgaataga atggcggaaa gaacaatgta ataatcaaca ggcaccatta gctttgcgct
     1081 cccttttcct gcttggtcaa cacgatcgcg ccgaccattg cgaccaacag tagaatggat
     1141 gtcacttcaa agggtagaag gaattgattg aagagggtca aggccatgtt tcgcagactg
     1201 tcaatggaat tcagtgttgc atccggcgag gcaatcactc cctccgggcg cgcacggaca
     1261 aaaagcaggt agatcgcttc aaccgccagg atgacagaca aggttgctgc aagcggtctc
     1321 tgccagggca gggtattggt aaccgcaaga ttttcagcgc caagcaacat gatcacaaag
     1381 aggaacagca ccatgatcgc accggcatac accgtgatct gcgacatggc aatgaatggt
     1441 gcgcccaaca gcagataaaa taccgccacg gtcacgaaat tcagcaccag gaagagcgcc
     1501 gaatatacgg cattacggct gaataacatt cccaatgcag ttgcgatggc aaccagggaa
     1561 agaacaagaa aaacaatcag ttcagaggtc atagaactcc attttcgatt tgcgattttg
     1621 gatttacgat ttttcaatcg caaatcgtga atcgtcaatc gtcaatcggt ggggtctttc
     1681 atctccggca ccgaacgggt gaatacaccg gtttccactt tctgcggtgt aaccatgttc
     1741 tcggcagaga caggctgtaa aagcatttcc tttgtgtaga tcgcatcacg gcggtcggtg
     1801 aaggatagct cgtagttatc gcccaatacg atcgcttcag ttgggcaggc atcttcacag
     1861 aaaccgcaat agatgcagcg cagcatgttg atctcatagg tggaggcgta acgttcgccg
     1921 ggtgagaagc gtttttcgtc ggtgttctcc gaggcttcta cgaagatcgc atccgcgggg
     1981 caggcggcgg cacacagcga acatccaatg cacttttcca ggccgttgtc gtaacgctgc
     2041 aagacatgac ggccacggaa gcgcggacga accggccgct tttcttcagg atattgatag
     2101 gtaacggtcg gctcacgcag gaaggagcgg aacgtctcgc ccaacccttt tgcaagttcg
     2161 aaaatgggat caaacacgcc catgggactt tctcctcaaa tcacgataaa cgaaggcaaa
     2221 tgccacaaag aaaccaatga tcgacacaac gggaatgctc cagaagaaca atggattatt
     2281 gaactcctgt tgcagcaaaa tgccgactgc ggttacaaaa gccaggaaca acgtcagcgg
     2341 cagaagcacc ttccaaccaa aagccatcaa acggtcgtag cggatgcgcg gccaggtggc
     2401 gcggatccag atcataccga aaaggatcac gatcac
//
LOCUS       DAOQNJ010000433         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34009_length_2433_cov_8.444491, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000433 DAOQNJ010000000
VERSION     DAOQNJ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34009_length_2433_cov_8.444491"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLG52_11235"
     CDS             complement(<1..335)
                     /locus_tag="PLG52_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNB42066.1"
                     /translation="MTTSDVVSAETLETKTKLNGKILKTTLAGALVDIGQSLPGVLHI
                     SQISETPVNKVEDVLKEGQEVTVWVKRVRKDRIELTMIEPLMYDWKELKPDMVVKGKV
                     TRLETYGAFV"
     gene            complement(495..1574)
                     /locus_tag="PLG52_11240"
     CDS             complement(495..1574)
                     /locus_tag="PLG52_11240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNB42067.1"
                     /translation="MSQPLNNQTRPFELGFYNFVDSGANPLTGEKKDPATVMKNMLEV
                     IELADQVGLDVFAIGEHHRVEYLASAPEVILGAAAARTKNIRLSTAVTVLSSDDPVRV
                     FQRFATVDLISNGRAEIMAGRGSFIESFPLFGYNLNDYDGLFDEKLRLLSKLNESTNI
                     TWTGNFRAPLNDLGIYPRPVQEKLPIWVAVGGTPESVVRAASMGLPMALAIIGGMPER
                     FAPYLDLYRQASAEYGFEPDTIPLSINSHGFIADTAEEAVKEYYPIQTYNMNKIGRER
                     GWAPATQDQLEKQRHLRGSDFVGTPDEIIEKILFQYDIFKHQRFLMYMGNNAIEHKKM
                     MHAIELFGTKVAPTVRKEIEKRRAG"
     gene            complement(1707..>2433)
                     /locus_tag="PLG52_11245"
     CDS             complement(1707..>2433)
                     /locus_tag="PLG52_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42068.1"
                     /translation="QVDGNDNILLFNYNSSDIQMFDPQGNELSVMKITDADGDEINNS
                     KMGVTSNGTIYVPGFQSIMSFSESGERLREIPNTDQFFIIHTLLVGADDKLYVRSSSG
                     ILRFDENNKVDLFIPDATLEDLSGQTPSIGALGVDAQGNIYFSGTFNKDVLKFSPTGE
                     FISSFGGDEFTSVRAIEIDSYGRIYIVDFSEVRVYDSNFTYLGSIDGAFWGVDFDSQG
                     YMYAVETNGDDVLKFEVKKPDAQ"
BASE COUNT          605 a          550 c          626 g          652 t
ORIGIN      
        1 acaaaagcgc cgtaggtttc gaggcgggtt actttgcctt tcacaaccat atcgggcttg
       61 agttctttcc aatcgtacat aagcggttcg atcatggtga gttcaatgcg atccttgcga
      121 acacgcttga cccacacggt cacttcctga ccttccttga ggacatcttc cactttgtta
      181 accggggttt cgctgatctg tgagatgtgg agcacgccag ggaggctctg tccaatatcc
      241 accaacgccc ctgcgagtgt ggtcttcaag atttttccgt tcaattttgt tttggtttcg
      301 agtgtctcag cggacacgac atctgatgta gtcataatta gctcctgctg tttgaaataa
      361 tgccgaagaa cgattatacc tttctgaaaa cgatgcgtca atgagtaaaa aggttagtga
      421 gggatgagaa tctctcggcg tattgtcatg tttgagcagg agaatcagga aagagcgcgg
      481 ttcgaagcgg acctttatcc tgctcggcgt ttttcgatct ctttcctcac tgtaggcgcg
      541 accttcgtcc cgaaaagttc gatggcgtgc atcatttttt tgtgctcgat ggcattgttg
      601 cccatataca tgaggaagcg ttgatgtttg aagatgtcgt attggaagag aatcttctcg
      661 atgatctcat ctggcgtgcc gacaaagtct gagccgcgca ggtgacgttg cttttcaagc
      721 tgatcttgcg tggcaggcgc ccagccgcgc tcgcgcccga tcttgttcat gttgtaagtt
      781 tgaattggat aatattcttt gacggcttct tcggcggtat cagcgataaa accgtgtgag
      841 ttgatgctca atggaatggt atctggttca aaaccatatt cggcagatgc ctgacggtaa
      901 aggtcgaggt agggagcgaa gcgttcgggc atgccgccaa tgatggcaag cgccatgggc
      961 agacccatcg aagccgcacg gacgactgat tcaggcgtgc cgccgaccgc aacccaaatt
     1021 ggcaactttt cctgcaccgg gcgcggatag atgccgaggt cattcagagg ggcacggaag
     1081 ttgccagtcc atgtaatatt ggtggattca ttgagcttcg agagcaagcg taacttttca
     1141 tcgaaaagac cgtcgtaatc gttgaggttg tagccgaaaa gcggaaagga ctcaatgaaa
     1201 gagccgcgcc cagccatgat ttcggcgcga ccattggaaa taaggtcgac tgtggcgaag
     1261 cgctgaaaga cgcgcacggg gtcatcggag ctgagtacgg ttacggcggt agagaggcgg
     1321 atgttcttcg tccgcgcggc ggcggcaccg aggataacct ctggcgcaga ggcgagatat
     1381 tccacgcggt gatgttcgcc aatggcgaat acatccaagc caacttggtc ggcaagctcg
     1441 atgacttcga gcatattttt catcactgtg gcggggtctt tcttttcacc ggtcagcgga
     1501 tttgcgccac tatcaacgaa gttataaaag cctaattcaa atgggcgagt ttggttattt
     1561 aatggttgag acattttgat ttccgattca atattaatgg tttctgtaga gacacagaag
     1621 cagctacagg cgaaagtctt actaaaaagc gacccttttt cgtagaagag ggtcgctttg
     1681 agaattataa attgcagaaa ttagggctat tgcgcatcgg gctttttgac ttcaaatttc
     1741 aagacatcgt cgccgttcgt ttcgaccgca tacatgtagc cttgtgagtc aaagtcaacg
     1801 ccccagaatg cgccatcgat actgccaagg taggtgaaat tcgaatcgta cactcgtact
     1861 tcagagaaat cgacaatata gatacgacca taggagtcga tttcaattgc gcgcacgctg
     1921 gtgaactcat cacctccaaa gctgctgata aattcacccg taggagagaa cttaagcaca
     1981 tctttgttga aagtgccgct aaagtaaata tttccttgtg catctacacc caaagcgcca
     2041 atgctgggag tttgacctga taggtcttcg agggttgcat ctggaatgaa taaatcgacc
     2101 ttgttatttt catcaaagcg cagaatacct gaactggagc ggacgtataa cttatcatcg
     2161 gcgccgacca ataaagtatg aatgataaag aactggtcgg tatttggaat ttctctaagg
     2221 cgttctccgc tttcactaaa gctcataatg ctttgaaaac cgggcacata gatcgtgccg
     2281 ttgctggtga cgcccatttt gctgttgttg atttcatccc cgtcagcatc ggtgatcttc
     2341 atgacggata attcattgcc ttgtgggtcg aacatttgaa tatcagaact gttgtaattg
     2401 aacaacagaa tattgtcatt cccgtccact tga
//
LOCUS       DAOQNJ010000434         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34035_length_2432_cov_7.014724, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000434 DAOQNJ010000000
VERSION     DAOQNJ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34035_length_2432_cov_7.014724"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLG52_11250"
     CDS             complement(<1..472)
                     /locus_tag="PLG52_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNB42069.1"
                     /translation="MKKIIIIGGGIGGLCTAIALQQYGFEVKVYEKVRKLGEVGAGLT
                     LWSNAIKVLRKLGVADAVISAGSKLERSQIRADNGETLFNARADEMEAKYGEPVIAIH
                     RADLHEILINALKPGTLHLDMNCLRFEQDDEKVTIHFENGEAETADLLIGADGIH"
     gene            complement(539..1045)
                     /locus_tag="PLG52_11255"
     CDS             complement(539..1045)
                     /locus_tag="PLG52_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42070.1"
                     /translation="MEFLSDILAWAWARHHNVLSWYIRPLFILPYIYFAYKRSWKGLV
                     ITVIALATSMFWFPAPETVDPMVEQFLQAERDYLLGTWTFGKVLLTLTVPAFFYFLAL
                     AFWKRSWWWGVAVINLAALGKIAWSVAEGGESGWAVLVPAVIGMLICDAAVYFGVKFI
                     NRNKTQTA"
     gene            1163..>2432
                     /locus_tag="PLG52_11260"
     CDS             1163..>2432
                     /locus_tag="PLG52_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42071.1"
                     /translation="MTLGKKYGMLQSMNREVPTQDQLPPRWQKAIQLGWGLFTLLVLT
                     FFFFEVSFSYKELLKPSIELQNNLAQLGMSVQTHALYLTGLRVAFMLVHLMIAIMIVV
                     KRPNERITIFVALTLVFMGITFWPPANRIATNIDIWQFLRSVAQFLSTIFLIIFFLIF
                     PDGHFTPRWSSFYVVAVLPLLAAEYFFPSSLLNPQNWNVLFSTALSLCTLAILVYVPV
                     HRYRHISNPTVRQQTKWVVYGISIALLGFFFIAFPYDFNYPQLQLGTLFSLFQVTGLI
                     FFFLLIPLSIGIAILRFRLWDIDPLINRTLVYGVLSFLIIAFYAFTVGIFAFYFRSNE
                     TNLIISFIATSIIAILFEPLRQRLQRGVNRLMYGERDDPATVLTRLSQRLDSALAPDS
                     VLQTIVETLAQTLKLPYAAISLLDEEPRFIS"
BASE COUNT          602 a          685 c          484 g          661 t
ORIGIN      
        1 aatggattcc atccgcgccg atcaacagat ccgccgtctc cgcttcgccg ttctcaaaat
       61 ggatagtgac cttttcatca tcttgttcga agcgtaaaca attcatatca aggtgtaaag
      121 ttcccggctt caaagcgttg atgagaatct cgtgcaagtc tgcgcgatgg atggcaatca
      181 caggctcgcc atactttgct tccatttcgt cggcacgcgc attgaacaga gtctcaccgt
      241 tatcagcgcg gatctgtgag cgttccaact tcgaacctgc gctgataact gcgtccgcta
      301 cgccaagttt gcgcaacacc ttgattgcgt tcgaccacaa ggtcagcccg gcacccactt
      361 caccaagctt cctcaccttt tcataaactt tcacttcaaa cccatattgt tgcagggcga
      421 tggctgtaca aagtccgcca atgccgccgc cgataataat gatcttcttc attctatagc
      481 tccaaaatta gggtatcgaa agtctaacat gggtagagtt tcgataccca tttattgttc
      541 atgctgtttg tgtcttatta cggttgataa actttacacc gaaatacacc gccgcatcac
      601 agatcaacat gccgatcacc gcagggacaa gcaccgccca acccgactca ccgccttcgg
      661 caacgctcca ggcgatcttg cccaatgccg ccaaattgat gaccgccacg ccccaccacc
      721 aggagcgctt ccaaaacgcc agggcgagga aatagaaaaa ggcaggcaca gtcagagtga
      781 gcaacacttt gccaaatgtc catgtgccaa gtagatagtc gcgctccgct tgcaggaact
      841 gctccaccat tgggtcaacg gtttcgggtg cagggaacca gaacatactg gtggcaagtg
      901 caattaccgt gataacgaga cccttccaac tgcgcttgta ggcgaagtaa atgtaaggga
      961 ggataaacaa cgggcggata taccaactca aaacgttgtg gtgacgcgcc caagcccaag
     1021 caagaatatc tgataaaaac tccatgtcaa aatctccttt tgttgaattt tcctaaagat
     1081 acaacgttcc catcccactg acatcggtct acgctcactt tgttcgcggg aagttttccc
     1141 aaaagtttgg gaatacgttc ccatgacact gggaaaaaaa tacggtatgc tccaaagcat
     1201 gaacagagaa gtgcccacac aagatcaact ccccccacgt tggcaaaagg ctatccaact
     1261 tggctggggt ttatttacgc tgctggtact taccttcttc ttttttgaag tgagcttttc
     1321 ttataaagag cttctcaagc catccattga attacaaaac aatcttgccc agctcggaat
     1381 gagcgttcaa acccacgccc tgtatctaac aggcttacga gtggctttta tgctcgttca
     1441 ccttatgatc gcgatcatga tcgtggtgaa aagaccaaat gaacgaatta ccatctttgt
     1501 tgccctcaca ctggtcttta tgggcatcac gttctggccc cccgccaacc gaatcgccac
     1561 caacatagat atctggcaat tccttcggtc ggttgctcaa ttcctaagta ctatcttctt
     1621 aattattttc tttctgatct ttccagatgg gcatttcaca ccccgctgga gcagcttcta
     1681 tgttgttgcg gttctgccac ttctggctgc cgaatatttt ttcccaagca gcctgttgaa
     1741 tccgcaaaac tggaatgtac tattctcaac cgctttatcg ctatgcacac ttgcgatttt
     1801 ggtttacgtg cctgtccatc ggtatcggca catttccaac cccaccgttc gccagcagac
     1861 aaaatgggtc gtgtacggca tctccattgc cctgcttggg tttttcttta tagcattccc
     1921 ctatgacttc aattaccctc aattacaact tggaactctt tttagtttgt ttcaagtaac
     1981 cgggttgatc tttttctttc tgctcattcc actttcaatt ggcattgcca tcttaagatt
     2041 ccgtttgtgg gatattgacc cgctcatcaa tcgcacattg gtgtatggcg tactcagttt
     2101 tttgatcatt gctttctacg cattcaccgt cggcatcttc gcattctatt tccgcagcaa
     2161 cgaaacaaat ctcattattt cattcattgc cacaagcatc atcgccattc tctttgaacc
     2221 gcttcgccaa cgcttgcaac gtggggtgaa tcgcctgatg tacggcgaac gcgacgaccc
     2281 agccaccgtc ctcacacgat taagccaacg gctcgactcc gccctcgcac ctgattccgt
     2341 tttacaaacc atcgtagaaa ctctcgccca aaccttgaaa cttccctacg ccgccatctc
     2401 tttattggat gaagaaccgc gcttcatttc ca
//
LOCUS       DAOQNJ010000435         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34092_length_2429_cov_10.176495, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000435 DAOQNJ010000000
VERSION     DAOQNJ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34092_length_2429_cov_10.176495"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLG52_11265"
     CDS             complement(<1..836)
                     /locus_tag="PLG52_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB42072.1"
                     /translation="MKKVFLFVSLLVITSLSCKALSPKPTEQTTPPTLSPASGDTQSD
                     TAAKLQELGGVTCEDAPDLTCVTIQVPLNHYDSGNTETIDVVFGVAPATGERYGMFVQ
                     AFPGGPGGEGISSSYMGYFDPTVLEHYDMVYYDQRGLGLSSPLSCPKAYAADFVEYLT
                     TPDTAGLEGLDTPEEQKTAIDDSRAYAQNCIAEMGIDPAKLAFYGTDQVAEDIDSFRA
                     LLGDEKLWIYGVSYGTAVAQTYATEHPDRVAGMILDGTINLTLDGVQGSLAQEKAFDK
                     VLV"
     gene            complement(856..1863)
                     /locus_tag="PLG52_11270"
     CDS             complement(856..1863)
                     /locus_tag="PLG52_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNB42073.1"
                     /translation="MSTVFTFVPSREHEYPDHPERPGRLDILEPLLPSFGAEQIQAIR
                     ATEADVALVHPVKMIRSLQEACMEGPGVIDYAPTYVTQTSYEDALKAVGGVLTCSHAV
                     MNGDARNAFAIVRPPGHHAEPDRAMGFCLFSNIAIAAQDLLRKGLKRVMVIDYDAHHG
                     NGTQAAFLHEERLGFVSTHQWGIYPGTGWIDDAPHANQRIVNVPLPAYAGDKTFVRIA
                     DEVFAPMVRAFKPEMLLISAGFDSHWNDPITSLGLSTRGFYDVSKKLVEMAEEHCGGK
                     VVFVLEGGYDPRNVANGVGAVFDALTSRPLGNEAGDASPYKEPDFESRLKEIRQKHDL
                     I"
     gene            complement(1965..>2429)
                     /locus_tag="PLG52_11275"
     CDS             complement(1965..>2429)
                     /locus_tag="PLG52_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42074.1"
                     /translation="FQIDVENLKLINAKNLGIKPAYYDHNLGQKMEFPAPGESSQLAQ
                     SLISNYGYLYYPLGVESDGACVEVLESTFNTKIILEASSIIMDGKSTGPYTWTIDYTY
                     LLNTTNNPVPNATFIVNSAIESTDALPTVPVISDAFWEEYAKFIWGKLTPKH"
BASE COUNT          619 a          598 c          592 g          620 t
ORIGIN      
        1 accaatactt tgtcgaaggc tttctcttgt gcaagtgaac cctgcactcc atccaatgtc
       61 aagttaatgg tgccatccag aatcatgcca gccacacggt ctgggtgttc ggtggcatag
      121 gtttgagcga ctgccgtgcc atagcttacg ccatagatcc aaagcttttc atcacccaac
      181 aaagcacgga atgaatcaat atcttccgct acttgatcgg tgccataaaa agctaacttg
      241 gcagggtcaa tgcccatttc tgcgatacag ttttgagcat aggcacgcga atcatcaatg
      301 gcggttttct gttcttcggg tgtatctaaa ccttccaacc cggcagtatc gggcgtggtg
      361 aggtactcaa caaaatctgc ggcatacgcc ttcgggcatg agagcggact agaaagccca
      421 agcccacgct ggtcataata gaccatatcg taatgttcaa gaaccgttgg gtcgaaataa
      481 cccatatacg atgaactgat gccttcgccg ccgggtccgc ctgggaaggc ttgcacgaac
      541 atcccatagc gttcacctgt ggcaggtgcc acaccaaaga ccacgtcaat ggtttctgtg
      601 ttgccagaat cgtagtggtt tagcggaacc tgaatggtca cacacgtgag gtcaggtgca
      661 tcttcacaag tcacaccgcc taattcctga agcttggcgg cagtatcact ttgagtatct
      721 ccacttgcag ggctgagggt gggaggagtg gtctgttctg tgggcttcgg tgataaagct
      781 ttacaactta aagacgtgat tacgagtaac gatacgaaca aaaaaacttt cttcatataa
      841 atttcctttt gatgtttaga taaggtcgtg cttttggcgg atctctttca agcgtgactc
      901 gaaatccggc tctttatagg gagatgcatc gcccgcctca ttccctaagg gacggctggt
      961 cagcgcatcg aaaactgcgc ctacgccgtt cgcgacgttt cgcggatcat acccaccttc
     1021 caatacaaaa acgacttttc ccccacaatg ttcttctgcc atttccacca attttttcga
     1081 cacgtcataa aagccacgtg tcgacaatcc caatgaagtg atggggtcat tccaatgcga
     1141 gtcaaaccct gctgagatca atagcatctc gggcttgaag gcacgcacca tgggcgcaaa
     1201 gacttcgtct gcgatgcgca caaaggtctt gtcacctgca taagccggca acggcacatt
     1261 gacaatgcgt tggttggcgt gaggcgcatc gtctatccag ccggtgccgg ggtaaattcc
     1321 ccattgatgc gtggagacaa atcccagccg ttcttcgtgc aaaaatgctg cttgtgtccc
     1381 attgccatga tgtgcgtcat aatcgatcac catcacacgc ttcaagcctt ttctcagcaa
     1441 atcctgtgca gcgatggcga tattgctgaa gagacaaaac cccatcgcgc ggtctggttc
     1501 ggcgtggtgc cccggcggac ggacgatggc aaatgcatta cgagcatctc cattcatcac
     1561 cgcatgagaa caggtcaaca cgccaccgac ggctttcagc gcatcttcat aagaagtctg
     1621 cgttacatag gtaggcgcat aatcaatgac accgggtcct tccatgcagg cttcttgcag
     1681 agaacgaatc atcttcaccg ggtggacgag agccacatcc gcttcggttg cccgaatcgc
     1741 ttggatctgt tccgcgccga aagagggtag caaaggctcc aaaatatcca acctgcctgg
     1801 gcgttcgggg tggtctggat attcatgctc ccgcgatggg acaaatgtaa atacagtaga
     1861 catgatttaa ttatagcccc ctgttggtga attcatgagc ttaagccaca gaagccatag
     1921 cggatttgag catttttaaa acccgctgtg gaaaaaagtt ttaattaatg tttcggcgtg
     1981 agcttgcccc aaataaattt tgcgtattct tcccagaacg catctgagat gacgggaaca
     2041 gtgggcaagg catccgtcga ctcaatcgca gaattaacga taaaggttgc attggggacg
     2101 gggttattcg ttgtgtttaa gagataggtg taatcaatcg tccatgtata tggtcccgta
     2161 gactttccat ccataataat ggaggatgct tcaaggatga tcttggtgtt gaaggtcgat
     2221 tcaaggactt ccacacaggc gccatcggat tctacgccta atggatagta caaatatcca
     2281 tagtttgata ttaaagattg tgcgagctga gaagattccc ccggcgcggg gaattccatt
     2341 ttttgcccaa ggttatggtc atagtatgca ggtttaatac cgaggttttt ggcattgata
     2401 agctttagat tttctacatc aatttgaaa
//
LOCUS       DAOQNJ010000436         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34111_length_2428_cov_9.064054, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000436 DAOQNJ010000000
VERSION     DAOQNJ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34111_length_2428_cov_9.064054"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLG52_11280"
     CDS             complement(<1..653)
                     /locus_tag="PLG52_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNB42075.1"
                     /translation="MNISSLLQGIASFAWIGFIGILVTISVRASRKQPVGGLSTAAII
                     LVVTAVLLTTVGAGIVFIEPDELGIVVTVLGSGGIRPDPLDSGLHWIVPFAERVERYP
                     KTNQTYTMTSVADEGQVAGDDSIQVRTKDGQQVYIDASVIYAVDPIKVVQLYSTWRFS
                     YEDQFVRTQARGVIRDVASQYGVEEIVSSKRAEMEQSIDDQLVAIFAENNLIMRDFV"
     gene            726..1400
                     /locus_tag="PLG52_11285"
     CDS             726..1400
                     /locus_tag="PLG52_11285"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42076.1"
                     /translation="MKTGTGKIEELFLDGSARISCPPELIPAPGQYLLAHAAGSDAPL
                     PVPLFSSLSSSQNGFRFAPPIPSSWQPGQLLHLRGPIGHGFSLPSSARKIALVGLEDT
                     PARLLGLLSQALKQSAEVVLVSNSSVEDLPEVVEIQPVKGLLDVLRWADYVAMDVNRE
                     KLNQLKEMLAGKDQITAKVEAQVLIHAPMPCGALADCGVCALSIDHDWKMICKDGPVF
                     ELKEIL"
     gene            complement(1430..2368)
                     /locus_tag="PLG52_11290"
     CDS             complement(1430..2368)
                     /locus_tag="PLG52_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42077.1"
                     /translation="MRKTFTPIVLTLLLLAAWIPVHAQSVETQYFSETGHNVSGDFLA
                     FYNSNPNAAYLYGYPITEQITGRDGKSVQYFQRARFEFRADLPEGQRVQLTQLGRETY
                     ISTGLLDVGNSFACRSYTETGFSVCFAFLEFFDANGGVAQFGYPISGFEYHENKIVQY
                     FERARLEWQPWRADGYRVVISDLGRAYFDKLGEDPALLAPASPLNNAPRVITELRIRA
                     FVWKAVTLSNDEQMFFVIVQDQNAQPVPGASCTAVVNWPNGAKDSTIITSDTNGVGVV
                     SLAFQNQPYGSLIYTDINCTYNNLTGKTTTSFRIWY"
BASE COUNT          646 a          610 c          582 g          590 t
ORIGIN      
        1 aaaacaaaat cgcgcataat caggttgttt tcagcaaaga tagctactaa ttgatcatca
       61 attgactgtt ccatttcagc gcgcttcgag ctgacgattt cttctacgcc atattgtgat
      121 gccacatcac ggatgacgcc acgtgcctga gtgcgcacaa attggtcttc atagctaaac
      181 cgccaagtgg aatacaactg caccactttg atcgggtcta cagcgtaaat gacggaagcg
      241 tcaatataaa cctgctgacc atctttcgtg cggacctgaa tagagtcatc cccggcaact
      301 tgcccctcat cggctactga cgtcattgta tacgtctggt ttgttttggg gtaccgctct
      361 acgcgttcag caaaaggcac aatccaatgc aaccccgaat caagcggatc tgggcgaata
      421 ccgccagagc ctagcaccgt cacaacgata ccgagttcat ctggttcaat gaacacaatt
      481 cccgcaccca cagtggtcaa caaaaccgct gtaaccacca aaataatcgc agccgtgctt
      541 aacccaccaa ccggctgttt tctactagcg cgcactgaga tcgtaaccaa aattccaata
      601 aaaccgatcc atgcgaaaga agcaatacct tgcagcaaac ttgatatatt catggttcct
      661 ccaattgatc tcgcaaaatt ataactggct tgtcatggct accgaagcat gttaatattt
      721 ttcaaatgaa aactggcaca ggaaagatcg aagaactttt tttggatggc tccgcccgga
      781 tctcatgtcc gcccgagctg attcctgccc ctgggcagta ccttctcgcc catgcagccg
      841 gctcagatgc acccttacct gttcctcttt tctcaagcct ttcctcatct caaaacggat
      901 tccgcttcgc gccgccaata ccttcttcct ggcagccagg tcagttacta catctacgcg
      961 gaccgatcgg gcatggtttc agcctgccgt cttcggcaag gaaaatagcg cttgttggct
     1021 tggaggatac ccctgcacga ttgctgggac tgctttcgca agcgttgaaa caaagcgccg
     1081 aagttgtatt ggtctccaac tcgtctgttg aagacttgcc cgaggtggta gaaattcagc
     1141 cggttaaagg gctacttgac gtgcttcgct gggcagacta tgttgccatg gatgtaaata
     1201 gggagaagct gaaccagcta aaagaaatgc tggcagggaa agaccaaatc acggcgaagg
     1261 tggaggcgca ggttcttatc cacgctccta tgccttgcgg cgcactggca gattgcggcg
     1321 tctgcgcctt gtccatcgat catgattgga aaatgatttg taaagatggt cctgtgtttg
     1381 agctgaaaga aatcttataa aaaaagcctc ccatttggga ggcttttgct tagtaccaaa
     1441 ttctgaagga tgttgtggtt ttgccggtta ggttgttgta ggtgcaattg atgtctgtgt
     1501 agatcaaact accataaggt tgattctgaa aagcgagcga gacgacgccc acgccatttg
     1561 tatcacttgt aatgatagtt gaatcttttg caccattggg ccagttgacc accgccgtgc
     1621 aagaagcgcc gggaactggc tgagcgtttt ggtcttgtac gatcacgaag aacatctgct
     1681 catcattcga gagggtaacg gctttccaga caaaggcgcg aattcgcaat tctgtgataa
     1741 cgcgtggagc attgttgagc gggctggcag gggcaagcag ggcagggtct tcaccaagct
     1801 tatcgaagta ggcacgaccc aaatctgaaa tcacgacacg atacccgtcg gctcgccaag
     1861 gctgccattc aaggcgcgcg cgttcgaagt actggacgat cttattctcg tggtattcaa
     1921 aaccagagat tgggtacccg aactgagcta caccgccatt ggcgtcgaaa aactcaagaa
     1981 aagcaaaaca gaccgagaag cctgtttcgg tatagctgcg acaagcgaag gaattaccaa
     2041 catccagcaa accggtggag atgtaggttt cgcgcccaag ttgcgttaat tgaacgcgtt
     2101 gcccttcggg caggtcggca cggaattcaa aacgcgcacg ttggaaatat tgcacacttt
     2161 ttccatcgcg accggtaatt tgttcggtta tggggtatcc gtaaaggtag gcggcgttag
     2221 ggttgctatt gtaaaaagca agaaagtctc cagagacatt atgtcccgtc tcactgaaat
     2281 attgagtctc tacagactgt gcatgaacag gaatccaagc agccaggagc agaagcgtca
     2341 gaacaatggg cgtgaaagtt ttcctcatta ctaccaccac tgtcattata ctcagaaaca
     2401 cccctcaaga cattacaaac agttatct
//
LOCUS       DAOQNJ010000437         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34112_length_2428_cov_8.987779, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000437 DAOQNJ010000000
VERSION     DAOQNJ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34112_length_2428_cov_8.987779"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1854)
                     /locus_tag="PLG52_11295"
     CDS             complement(<1..1854)
                     /locus_tag="PLG52_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB42078.1"
                     /translation="MTGELKEQFNHPNSEQNNDTIFQDAVNALRSGDKPRAKELLTLL
                     LKTDQNNPTYWIWLSASVDANKERIYCLQTALKLDPENSIAKRGLILLGALSPDETIQ
                     PFPMNRPRAWEEKLLLAHEKPKERGVRVVMRSPLVRLGGLAIIGIGLCAVIYFGVILP
                     RWGTIQLAPTNTPGPSPTFTATPTIFGATAIPTQVFSGPTPLSAFLNATYTPTALYVN
                     TPRSAVSVDQFRIAKQAYDNKDWDVFIENMLLIEPLEPDSVDIPYYIGEAYRFKGDTT
                     SAARYYRDAMNMNPAFGPAYLGLARVSLMSNPDFNPEKLFNQAIEYDPNLGEAYLERA
                     RWLIRRGDYADALPDLELADKIMPGSPEVYLAYANSYLGDGDKEKALEYAQKAYEADV
                     LNLPTYKLLGDLYIEKEDYANAAEALRLYTTYESQDATGFGKLGEAYYYLGEYQNAVL
                     AIDAGEQLNRNGMKKYMIYRGLSHVELGNFNEAEGDLNIAVDDEPESFEARLGYAKAL
                     YGVSKFGSAFLQAESMRSLADTDEEKALELYWRAKIQEQRNDIRDAIKSWNDLLKLDE
                     SVMTPEMRQEAQEHLKTLVPPTNTPKGGAPTSTPVPVTKTPQIGTTPTVGKT"
     gene            complement(1864..>2428)
                     /gene="amrA"
                     /locus_tag="PLG52_11300"
     CDS             complement(1864..>2428)
                     /gene="amrA"
                     /locus_tag="PLG52_11300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNB42079.1"
                     /translation="LTDKERQTLLHIARVSIESAVKGNPQPTLEKDTLTPALRENGAS
                     FVTLTINGELRGCIGALEAYQPLAEDVREHAVAAALNDPRFPVLREAELSRIQIEVSY
                     LTKPQELQYSTSQDLLAKLHPHRDGVILKYGRRRATFLPQVWEKLPKPEEFLSQLCYK
                     MGERQTLWQETKLQVYVYQVEEFHELN"
BASE COUNT          588 a          582 c          596 g          662 t
ORIGIN      
        1 ggtctttcca actgtcggtg tagtgccaat ttgcggggtt tttgtgacag gcaccggtgt
       61 tgatgtggga gcgccgcctt tgggtgtatt tgttggcgga acaagggtct tgaggtgctc
      121 ctgtgcttcc tgacgcattt ccggcgtcat cacgctttca tcaagcttca acaagtcgtt
      181 ccaagattta atcgcatcac gaatgtcatt tcgttgttct tgtatcttcg ctcgccaata
      241 cagctcaagc gccttttctt catctgtatc tgccagcgag cgcatggact cagcctgcaa
      301 gaaagcactg ccaaatttgc taacaccata cagggctttg gcgtacccaa gccgggcttc
      361 aaaggactcg ggttcatcat cgaccgcaat attgaggtca ccttcggctt cattgaaatt
      421 gccaagttcc acatgcgata agccgcggta gatcatgtat ttcttcatgc cattgcggtt
      481 gagttgctca cccgcatcga ttgcaagcac agcattctga tactcgccaa ggtagtaata
      541 cgcctcaccc agcttgccaa aaccagtagc atcttgagac tcataggtcg tatacaaacg
      601 caacgcttct gctgcattag catagtcttc tttttcaata taaagatcgc ctaaaagctt
      661 atacgttggg aggttgagaa catcggcttc gtacgctttt tgagcatatt ccaatgcctt
      721 ttctttatca ccatctccaa ggtaggaatt tgcatacgca agatacactt caggcgagcc
      781 tggcatgatc ttgtctgcaa gttccaaatc tggcagggca tcggcataat cgccacgccg
      841 aatgagccac cgagcacgtt caagatacgc ctcgcccaaa tttgggtcat attcaatggc
      901 ttgattgaat aatttttcag ggttgaaatc tgggttcgac atcagactta cgcgggcaag
      961 ccctaaatat gccgggccaa aggcaggatt catattcatt gcatcgcggt agtaacgcgc
     1021 tgcgctggtg gtatctccct taaaacgata ggcttcgcca atgtaataag gaatatctac
     1081 tgagtctggc tcaagaggtt cgatcagaag catattctca ataaagacat cccagtcttt
     1141 attatcatag gcttgctttg cgatacggaa ctgatccact gagacggctg aacgtggagt
     1201 attaacatac aaagcggttg gcgtgtacgt tgcattcaaa aaggcagaaa gcggagtcgg
     1261 tccgctaaag acctgggtag gaattgctgt tgccccaaag attgttgggg ttgcagtaaa
     1321 cgtaggagaa ggacctgggg tgttggtggg agcaagttga atcgtgcccc aacgcggaag
     1381 gataacccca aaataaatta cagcacacaa accaatgcca ataattgcca acccgcccag
     1441 ccgtaccaac gggcttcgca tgacgacgcg cacgccacgc tcttttggct tttcatgagc
     1501 aagcagtaac ttctcttccc acgcccgcgg acggttcata gggaagggtt gaatggtctc
     1561 atctggcgaa agagctccga gtagaatcaa gccgcgtttg gcgattgaat tttcggggtc
     1621 cagtttaaga gccgtttgta aacaatagat gcgttcttta ttggcatcca ctgaggcgct
     1681 tagccagatc caatacgttg ggttgttttg gtccgttttt aagaggaggg ttagcaactc
     1741 tttggcgcgg ggtttgtctc cgctgcgcag agcattcacg gcatcctgaa aaatggtgtc
     1801 gttgttttgt tcgctattag gatggttaaa ttgctctttg agttcgccag tcatgcgtcc
     1861 attttaattc aattcatgga attcttcgac ctgatataca taaacttgca attttgtctc
     1921 ctgccaaaga gtttggcgct cccccatctt gtagcaaagt tgacttagaa attcttccgg
     1981 cttggggagc ttctcccata cttgtggcag aaaggtggcg cggcggcgtc cgtatttcaa
     2041 aatgacccca tcccgatgtg ggtgtagttt ggcaagtaaa tcttggctag tggagtactg
     2101 caactcctgc ggttttgtga gataagaaac ttcgatttga atacggctca attccgcttc
     2161 acgcaggact ggaaaacgcg ggtcgtttaa cgccgctgca acggcatgct cacggacatc
     2221 ttctgcaagg ggttgatacg cctccaaagc gccaatacaa ccgcgcagtt cgccattgat
     2281 cgtgagggtc acaaaagatg ctccgttttc acgcaaagca ggtgtaagcg tatctttttc
     2341 aagagttggt tgagggttcc cctttacggc actctcaata gacactctgg caatgtgcaa
     2401 aagagtttgt ctttccttat ctgtcaac
//
LOCUS       DAOQNJ010000438         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34278_length_2421_cov_9.926458, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000438 DAOQNJ010000000
VERSION     DAOQNJ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34278_length_2421_cov_9.926458"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="PLG52_11305"
     CDS             <1..1386
                     /locus_tag="PLG52_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB42080.1"
                     /translation="RVIQEHPKSVGFPAHHPKMVSFLGVPIRTAEGQIGQIYLTEKLD
                     ADEFSSDDEMIIQMLATYAATAIANARLIDQMKERDVALTRRNVDMTLLNDIASTLTS
                     SLELDEILNKTLGLVMNYMKVEAGEIFLLEEDKTTLRMMLHRGQAAEAFWTHNIFRVG
                     EGYPGVVAETCRPLICTDLANDPNFQREAVVKAGFHQVLSFPLVSGERVMGVMSIATR
                     SKAAFEDRSVQMLTAVGAWAGLSIENARLHVNARRLAVLEERNRIGMDLHDGIIQSIY
                     GVGLALEGTKLLVDEDASSAKEKIDNAIDGLNQAIRDLRAYILDLRPRQLGTEGVISG
                     IKRLIAEYRANTFSEVTFMPPDSDLKELTQTQALALFHICQEALANAAKHAKAKNVQI
                     SLWTTEDRALLELHDDGKGFNTDEMSTFIGHGLANMQTRARSSGGDIDITSVQGEGTT
                     VFVWVPRGIKQ"
     gene            1922..2005
                     /locus_tag="PLG52_11310"
                     /pseudo
     CDS             1922..2005
                     /locus_tag="PLG52_11310"
                     /inference="COORDINATES: protein motif:HMM:NF023069.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2204..2421
                     /locus_tag="PLG52_11315"
                     /pseudo
     CDS             2204..2421
                     /locus_tag="PLG52_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
BASE COUNT          677 a          543 c          560 g          641 t
ORIGIN      
        1 cgggttattc aagaacatcc aaagtctgtt ggtttccctg cacatcatcc aaaaatggtt
       61 tcatttttag gtgttccaat ccgcacagca gaagggcaga tcggtcagat ttatttaacc
      121 gaaaaactag atgcagatga attctcgtca gatgacgaga tgatcatcca aatgcttgcc
      181 acatacgccg caacggcgat tgcgaatgca cgcctcatcg atcagatgaa ggaacgcgat
      241 gtagcgttga cgcgccgcaa cgtggatatg actttgctga acgacatcgc ctccaccctg
      301 acctcaagcc tggaactgga cgagattctg aacaaaacgc tcgggcttgt gatgaactac
      361 atgaaagtag aagccggaga aattttcctt ttagaggaag ataaaactac actgcgcatg
      421 atgttacatc gtgggcaagc tgctgaagcc ttctggacgc ataatatctt tcgcgtaggg
      481 gagggatatc ctggcgtagt ggctgaaact tgccgtccgc tcatttgtac agacctggca
      541 aacgacccaa attttcaacg tgaagcggta gtgaaggcgg gatttcatca agtattgagc
      601 ttcccgcttg tttctggcga acgggttatg ggggttatga gcattgctac ccgttctaaa
      661 gctgcatttg aagatagaag tgtccaaatg ctcacggctg tgggcgcttg ggcagggctt
      721 tcgattgaaa atgcacgttt acacgtcaat gcacgtcgtt tagccgtgtt ggaagaacgc
      781 aatcgcattg gtatggacct tcacgatggc atcattcaat ccatttatgg ggttggtctt
      841 gcgctggaag gtacaaaact cctagtagat gaagatgctt catctgcaaa ggagaaaatt
      901 gataacgcca ttgatggctt aaaccaagca attcgcgatt tacgagctta tattcttgat
      961 ctacgaccac gtcaacttgg tacagaaggc gtaattagtg gaattaaacg cttaattgct
     1021 gaatatcgtg ccaatacatt ctctgaagtc acattcatgc cacctgattc agacctcaaa
     1081 gaactcactc aaacgcaagc cttggcactt tttcatatat gtcaggaagc attagccaat
     1141 gcagccaagc atgccaaagc aaaaaatgtt caaatttcac tttggacaac agaagatcgt
     1201 gctttgcttg agcttcatga tgatggaaaa ggcttcaaca ccgatgaaat gtcgacattt
     1261 attggtcatg gtctcgcaaa tatgcagact cgtgcacgtt catcaggtgg tgatattgat
     1321 ataacatcgg ttcaagggga aggaacgacc gtatttgtat gggttccacg cggtatcaaa
     1381 caataaatca tgattgttgc cgaatcattg ccgggtgaat cacaaaaact cgtgtttcac
     1441 actttttgat tcagataagt tttgcattat gattctttaa gatagatatg ccaagcctgt
     1501 gtcgaaaacc ccttaaatac ccatatatgg gggtcggttc ttggttcagg ggcgttcttg
     1561 gctgaaacgc ttgttctaca aaaaatgtag aaagaaagta aacagataac aattctttaa
     1621 cattgatgaa tcttaaaaaa aacctttttt ttaacttagc gtaggttgaa cgactgttac
     1681 atatatgaat gttctgctag aatatgacca cgctgatata acggatgttt gcccgtacga
     1741 ttgacatccg caatccctaa aggatacttg acaaaacatg gatggaaatt caagccgctg
     1801 ttgggcgggg tggtttcatc tacgcaaaaa acaagcaaga ccataaaatt ttgtaaattg
     1861 aggaacgttt atgaacgctg agcaagcatg gcagaccgtc cttgcacaac tccaaatgga
     1921 tatgccgcgt gcatcctatg atacgtgggt gcgcgatacc cgtcccgttg cctatgaaaa
     1981 cgggatgtta accgttgggg ttcgcaatgc ctatgcccgt gactggctcg atagccgcct
     2041 tgccaccact gttaacaagc ttttgatcga cacgctgaac tcaaaagtct ctgtgaaatt
     2101 tgttgtttcc caatcggaag ataacgcctc tattgcggac ctcgaaactg ctcctgcttc
     2161 gatagagatt accccgccgg aaccaaaacc ccgtcatgta acgctcaatc cgcgctatac
     2221 atttgatacc tatgtggtcg gttcgggaaa ccgcctcgca catgcggctt gtcaggcagt
     2281 ggctgagaaa ccagcacgtg catacaaccc gctcttcctg tatggaggcg tgggactcgg
     2341 caagacccat ctcttacatg ccattggtaa tgcctgtcat gccagcggac tcaacgtcct
     2401 ctatgtttct tctgaagaat t
//
LOCUS       DAOQNJ010000439         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34414_length_2416_cov_8.272342, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000439 DAOQNJ010000000
VERSION     DAOQNJ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34414_length_2416_cov_8.272342"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLG52_11320"
     CDS             complement(<1..389)
                     /locus_tag="PLG52_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB42081.1"
                     /translation="MFGSKTQALQQRAQKVAPLGVNSNFRFWGDGITPYVEKAKGGYL
                     WDVDGKKYIDYRMAFGPIILGHAYDEVDQKVIEEIQKGILFAMTGELEIAVAEMIVDM
                     SPAIDMVRLACSGTEATMHAIRVARAYT"
     gene            complement(435..941)
                     /locus_tag="PLG52_11325"
     CDS             complement(435..941)
                     /locus_tag="PLG52_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB42082.1"
                     /translation="MYEFDKTDIKIVNLLLEDGRMSASEMSRRMGDISERAVRYRVDR
                     MIEEGVIQISAVVNPEALGFNIKADVWLEVEADAVLEVAKKMASFENVTYVACGIGQN
                     DISIQLVARDTSEIYYFVTEVVRKVPGVRKTTTSIVPIILKDVYQWRVPERIAKELSE
                     NETVTASK"
     gene            1074..>2416
                     /locus_tag="PLG52_11330"
     CDS             1074..>2416
                     /locus_tag="PLG52_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNB42083.1"
                     /translation="MKISLLEIKEPKILKASEIKIELTTTPVKYYRHGWQSWSLTAWT
                     DLSPLPMQKPAIFHPLQTDAVYANEKNPHGSWLGAVELEDGKILLLGALATDTHVFLV
                     GNQLEGRSEAGEVEWLIAIGEEKTVFDEYVHQLGLRFGRVDKNHIPRVWCSWYSLYTA
                     IDEKILHESFDGLGNLPFDVLQVDDGWQNDIGDWEANEKFPSGMQALAEKIKSTGRRA
                     GLWLAPLLVAKSSRLYREHKDWLLRDEKGRFVSAGFNWGQQLYALDTTHPDVISWLVA
                     LMKLVRRWGFDYYKLDFLYAGALKGKRYKDMPREAAYRECLRYMREAMGEGSFFLTCG
                     TPILPALGMCDAIRIGPDVSHEWENYRNETLLQNFATPGTKNAIRTVVHRLWLKPLVH
                     IDPDVEYFESKENSLADEHKQQLQDLALICDFKATSDLPQWMTSEEREKVRTFLDA"
BASE COUNT          641 a          626 c          542 g          607 t
ORIGIN      
        1 gtatacgccc gcgccacacg aatagcatgc atggtcgcct cagtccctga acaagccaag
       61 cgcaccatgt caatagctgg ggacatgtca acgatcatct cagccacggc aatttcaagt
      121 tcgccggtca tggcaaacaa aatgcctttt tgtatttctt cgataacttt ttggtctact
      181 tcgtcatagg catgccctaa aataatcgga ccaaatgcca tacgatagtc aatatatttc
      241 tttccatcta catcccataa atagccgccc ttggcttttt caacataggg agtaatgcca
      301 tctccccaaa aacgaaaatt agagttcacc cccagcggtg ccactttttg cgcacgctgt
      361 tggagagcct gagtctttga accgaacatg attgctcctt ttatcgggtt gcggcgaatc
      421 gccgcgtagc agaattactt ggatgcggtt actgtttcgt tctctgacaa ttctttggct
      481 atgcgctctg gcactctcca ctggtaaaca tctttgagaa tgatcggtac gattgaagtt
      541 gtggtcttgc gcacccctgg aaccttgcgc accacttcgg tgacaaaata atagatctcc
      601 gaagtatcac gcgcaaccaa ctgaatactg atgtcattct gcccaatgcc gcaggctacg
      661 tacgtcacat tttcaaaaga tgccattttc ttggcaactt ccaaaaccgc gtcggcttct
      721 acttccagcc acacatcggc tttgatgttg aatccgagcg cctctgggtt gaccaccgca
      781 ctgatctgaa tgacaccttc ttcgatcatt cgatccacac ggtaacgtac cgcccgttcc
      841 gaaatatccc ccatccggcg cgacatctca gaagcggaca tacgcccatc ttctagaagc
      901 aaattgacga tttttatatc agttttgtcg aattcataca taggatttag gcttcttttc
      961 cgaaagtgac acgcagaata taccttacgg cattttagac gtcaagggag caaaattggc
     1021 gtagttacgc attttccttt acaatcagcg cgaaatttga tggaaactca aacatgaaaa
     1081 tctctctttt agaaatcaaa gaacctaaaa ttctcaaagc cagcgaaatc aagatcgagc
     1141 tcacaaccac cccggtaaag tattaccggc atggctggca gtcttggtct ctcacagcat
     1201 ggacggatct ctctccactc cccatgcaaa aacccgccat atttcaccca ttacaaaccg
     1261 acgcggttta tgcaaatgaa aagaatccgc atggttcatg gctaggcgca gttgaacttg
     1321 aagatggcaa aatccttttg cttggcgcac ttgctacaga tacgcatgtg tttctggtgg
     1381 gcaatcaact cgaagggcga agtgaagcgg gcgaagtcga atggcttatt gctataggcg
     1441 aagagaaaac cgtcttcgat gaatacgtcc atcaactagg gttgcgattt ggcagagtag
     1501 ataagaatca cattccacgt gtatggtgct catggtatag cctttacaca gccatcgacg
     1561 agaaaatctt gcacgagtct tttgatgggc tcggaaacct gccttttgat gtgctgcaag
     1621 tggatgatgg ttggcagaac gacattggcg attgggaagc gaatgaaaaa tttccatcgg
     1681 gtatgcaggc attggcagag aaaatcaaat ccaccgggcg acgcgccggg ttatggctcg
     1741 ctccattatt agtggcaaaa tcatcacgcc tctatcgtga acataaggat tggttattgc
     1801 gcgatgaaaa gggacggttc gtttcagctg gattcaactg gggtcagcaa ctttacgcgc
     1861 tcgacaccac gcatcccgac gtaatctcgt ggctggttgc gctcatgaag ctggtgcgcc
     1921 gctggggctt tgactattac aaactcgact tcctttatgc cggtgcactg aaaggcaagc
     1981 gctacaaaga tatgccgcgt gaagctgcat atagagaatg tttgcgctat atgcgcgaag
     2041 cgatgggaga aggctccttc ttccttacct gtggtacgcc catcctccca gccctcggca
     2101 tgtgcgacgc catccgcatc ggacccgacg tttcgcatga atgggaaaat tatcgcaacg
     2161 aaactctctt acagaacttc gccacacccg gaaccaagaa tgccatccgc accgtggttc
     2221 atcgtttgtg gctcaagccc cttgtccaca ttgaccctga tgtggaatat tttgaatcaa
     2281 aagaaaattc actcgcagac gaacataaac agcaactaca agacctcgcc ctgatttgcg
     2341 acttcaaagc cacctcggac cttccgcaat ggatgacctc ggaggaaaga gaaaaagtcc
     2401 gcacatttct ggatgc
//
LOCUS       DAOQNJ010000440         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34482_length_2413_cov_10.202714, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000440 DAOQNJ010000000
VERSION     DAOQNJ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34482_length_2413_cov_10.202714"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLG52_11335"
     CDS             <1..523
                     /locus_tag="PLG52_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42084.1"
                     /translation="PNWFHEFVGSMLHEHEGELAFNIVPLATSLIVALGGLTAGYFAY
                     RNVNSPAEDKLQIPVLKNKWYVDELYNVVFIKPAVWFSEVFVSKWMDKGFIDGILHLF
                     GPATGGVGSAIRNYFDLPVINHFLGDGTADVTWWFGRNLRPIQTGRIQQYLILTLIVL
                     FVVGGLLYFLLMA"
     gene            533..2053
                     /locus_tag="PLG52_11340"
     CDS             533..2053
                     /locus_tag="PLG52_11340"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNB42085.1"
                     /translation="MEYFNTHLLSLILFIPAISAVIMLFLPNEAQLQRWFAFVASLIP
                     FVLTLVLWSKFDPNATGFQFEEKYVWYEAIHSSFHIGVDGISFSMVLLTTFLTPLAIL
                     ASFSVSDRVKPYMMLFLFLETGMLGVFLALDLLIFFVFWEIGLVPMYFLINQWGSANR
                     DYASLKFMIYTMGGSLGLLLAVQMLGVLFGTYDLVSLYQQWNSLTADTVLMGMTVGTV
                     KTIAFWAFAIAFAVKVPLWPFHTWLPDAHTEAPTAGSMILAGVLLKLGAYGFIRLVLP
                     LYPVEAKLFAGALAVLAVGAIVFGAFGSFGQTDFKRLVAYSSVNHMGFVVLGIAAAGL
                     AAGTADAHIAMSGAVLQMFNHGLSAAGMFFLVGVIYERTHTRNLDEFGGLFPLVPVYG
                     GILIFTSMASLGLPGLNGFVSEFMIVRGSYQPLTIYTAIAMLGLLFTGAYILKGIKKV
                     LHGPMNEHWAHGEHKLTEINLREILVMVPLMALMLLTGIWPMWILDIINKAVTALF"
     gene            2061..>2413
                     /locus_tag="PLG52_11345"
     CDS             2061..>2413
                     /locus_tag="PLG52_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42086.1"
                     /translation="MMNFPILSVITFLPFAAGMLMLMMPANRKNEVRAVALASAVIDL
                     LLSAWVYLQYLLNHMQGYQFIEKYTWLNIPPLNIALHFGVDGMSAPLVLLTGIVMFTG
                     VLISWGNDDPHVMSG"
BASE COUNT          455 a          629 c          595 g          734 t
ORIGIN      
        1 cccgaactgg ttccatgaat ttgtcggctc gatgttgcat gaacatgaag gcgaacttgc
       61 tttcaatatc gttccgctgg caacctcttt gattgttgcg cttggtggtc ttacagccgg
      121 ttactttgcg tatcgtaatg tcaactcacc ggcagaagac aaactacaaa tccctgttct
      181 caagaacaaa tggtatgtgg atgaactcta caatgtcgtg tttatcaaac ctgctgtgtg
      241 gttctctgag gtcttcgttt cgaagtggat ggataaaggc ttcatcgacg gcatcctcca
      301 cttgttcgga cccgctaccg gcggagtcgg aagcgcgatc cgtaattact ttgatctgcc
      361 cgtcatcaat cacttcctcg gcgatggaac tgccgatgtg acttggtggt tcggtagaaa
      421 tcttcgcccc atccaaacgg gacgcatcca gcagtatctg atcctcaccc taatcgttct
      481 gttcgtggtg ggtggtctgc tgtacttcct tttgatggca taggagcaaa cgatggaata
      541 tttcaataca catctcctta gcctgatcct tttcatccct gccatttctg cagtgatcat
      601 gctctttttg ccgaatgaag ctcaacttca acgttggttt gctttcgttg ccagcttgat
      661 tccttttgtg cttacactcg tgttgtggag caagttcgac ccgaacgcca ccggctttca
      721 gttcgaagaa aaatatgtct ggtacgaagc cattcattcg tccttccata ttggtgtgga
      781 cggtatttcg ttctcgatgg ttttgctcac caccttcctg accccgcttg ccattcttgc
      841 ttcgttcagc gtgagtgatc gcgtcaagcc atatatgatg ctcttcctgt ttcttgaaac
      901 aggcatgctc ggcgtctttt tggctctcga cctgctcatt ttctttgtgt tctgggaaat
      961 tggtcttgtg ccaatgtact tcctcattaa tcagtgggga agtgccaatc gtgactatgc
     1021 ttcgctgaag ttcatgatct acaccatggg cggatcgctc ggcttgttgc ttgcggtgca
     1081 gatgttaggc gttcttttcg ggacctatga cttggtcagc ctctatcaac agtggaattc
     1141 gctgacagcg gacactgtcc tcatgggcat gacagtaggt actgtaaaga ccatcgcttt
     1201 ttgggcgttt gccattgcct tcgctgtcaa agttcctttg tggcccttcc atacttggtt
     1261 acctgatgca cataccgaag ctcccaccgc tggttcaatg atcctcgcgg gtgtattgct
     1321 caagttgggc gcttacggtt tcatccgctt ggtgttgccg ctttaccctg tagaagccaa
     1381 gctgtttgcc ggcgctttag ccgttctcgc tgtgggtgcg attgtcttcg gtgcctttgg
     1441 ttccttcggt cagacagact tcaaacgctt ggtcgcctat tcgtctgtga accatatggg
     1501 ctttgtggtc ctgggcatcg ctgcggctgg attagccgcc ggcactgcgg atgcgcacat
     1561 cgccatgagc ggcgcagttt tgcaaatgtt caatcatggt ctctctgcgg cgggcatgtt
     1621 cttcctcgtg ggtgttatct acgaacgcac ccacactcgc aacttggatg aattcggcgg
     1681 cttgttcccg ttggttccag tctatggcgg catcttgatc ttcacctcca tggcttcgct
     1741 tggtttaccc ggtttgaacg gctttgtctc tgaatttatg attgtgcgag gctcttatca
     1801 gccgctgacg atctacaccg ccattgccat gcttggcttg ctctttacag gtgcttatat
     1861 cttgaagggc atcaagaaag tgttgcatgg acccatgaac gaacactggg cacatggcga
     1921 acacaaactg accgagatca acctgcgcga gattctcgtg atggttccgc ttatggcgct
     1981 gatgttgctc actggcatct ggccaatgtg gatcctcgat atcatcaaca aagccgtgac
     2041 ggctctgttc taagaggtgg atgatgaatt ttcccatcct aagtgttatc accttccttc
     2101 catttgctgc cggcatgctc atgttgatga tgcctgccaa ccggaagaat gaagtccgcg
     2161 cggtggctct ggcttcggca gtgatcgact tgctgctctc tgcctgggtt tacctgcaat
     2221 acctgctcaa tcatatgcag ggttaccagt tcattgaaaa atacacctgg ctcaacatcc
     2281 ctccgttgaa tatcgctttg cacttcggcg tcgacggtat gagcgctccg ctcgttttgt
     2341 tgaccggtat cgtcatgttc accggtgtgc tcatctcatg gggcaacgac gatccccacg
     2401 ttatgtctgg cat
//
LOCUS       DAOQNJ010000441         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34858_length_2398_cov_10.454119, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000441 DAOQNJ010000000
VERSION     DAOQNJ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34858_length_2398_cov_10.454119"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PLG52_11350"
     CDS             <1..729
                     /locus_tag="PLG52_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB42087.1"
                     /translation="VLLIHGFTGGPKEMRWMGDYLNQQGYTCLGIRLAGHATTPEDMI
                     RSRWTDWTASVEDGYNLLRGATDKIFLVGLSMGGVLSLLMSTRLDVAGVVAMSTPYQL
                     PRDEKDYSPEFIEFYSRFVKFMPKSTEVPGASWFDKEAFQSHISYPQNPIRSIAEMKR
                     LILVMRAALPNVTKPVLLIHSKNDKYVLPENVERLYDGLVNASDKTKAYVVEAGHVVT
                     RDAARHQVFEMARDFIRRVDASHS"
     gene            750..1877
                     /locus_tag="PLG52_11355"
     CDS             750..1877
                     /locus_tag="PLG52_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB42088.1"
                     /translation="MNRNLSFIAAALLLWGFGEGMFFNFVPIYLDVQFLLSKYEIGLI
                     LGGFGFFMAITHIPAGRLADRLGRKPLLIVAWLLGLLSTLTMGSAKTLPFFLIGLFTY
                     GLTAFVSSPLSSYVTAARGQLPVATVLSLTTATFGMGMVLGPVTSGWIGEHYGMRMSF
                     YAAAIVFVFSNIFVYFIGSQPIDHHDPSAPPLGLGSNKKFVTLMFVLAFAIFSMYLAQ
                     PLTPNFLEGVRGLSLSQTGLIFTIGALGNSLMAITFSRVNPRYGFLAAQSLVILFAVL
                     IWYGTSLPFFALGYFLLGGFRAGRPMALAQARELVHESQMGVTYGVMETVSAIITIIT
                     PPIAGFLFEFDPFIVYPISIGLLVLSILLSSVFLPRKESHA"
     gene            1870..>2398
                     /locus_tag="PLG52_11360"
     CDS             1870..>2398
                     /locus_tag="PLG52_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB42089.1"
                     /translation="MLEIVQFTLGPASTNAYLVADPETKEAVVIDPSWDGHLILAEAQ
                     KRGWRIGHLWYTHAHFDHIGGAAAIADAMNPLPHVALHPDDHVLWREGGGGKKFGLDI
                     DPGPEPTIDLIHGMTMKLGSNDFQVRFTPGHTRGHCVLYVAKESICFCGDLIFNESVG
                     RTDLPGSSFSALEKSI"
BASE COUNT          539 a          575 c          604 g          680 t
ORIGIN      
        1 gtattgctga ttcacggctt cacgggcgga cccaaggaaa tgcgctggat gggggattat
       61 ctcaatcaac aaggatatac ctgcttaggc atccgactcg cggggcatgc aacaacgccc
      121 gaagatatga tccgctcgcg ttggacggat tggacggctt ctgtggaaga cgggtacaac
      181 ttgctgcgtg gtgcaacaga caaaatcttt ttagtggggc tttctatggg cggcgtgttg
      241 tcgttgttaa tgtcaactcg tttggatgtg gcgggtgtag tggcaatgtc cacgccgtat
      301 caactgccgc gcgatgaaaa agattattcg ccggaattca ttgagtttta cagtcgcttc
      361 gtaaagttca tgccgaaatc aaccgaagtg ccgggcgctt cctggttcga taaagaagca
      421 ttccagagcc atatttcgta tccacaaaat cccattcgtt cgatcgcaga aatgaaacgg
      481 ctcatccttg tgatgcgggc ggcattgccc aatgtgacca agcccgtatt gcttattcac
      541 tcaaagaatg acaagtatgt tctgcccgaa aatgtggagc ggttgtacga tggactggtc
      601 aatgcatcag acaagacaaa agcctatgtg gtcgaggcag gtcacgttgt gacaagggat
      661 gcggctcgtc atcaagtctt tgaaatggcg cgggatttta tccgacgagt agatgcgtct
      721 cactcgtaag attttcaaaa gaggaatcgt tgaatcgtaa tctttctttc attgctgcgg
      781 cattgctgtt atggggattt ggcgaaggca tgttcttcaa ttttgtgccg atctatttgg
      841 atgtgcaatt tttgttaagt aaatacgaaa ttgggctcat tcttggcggg ttcggctttt
      901 tcatggcgat cacacacatc ccggctggaa ggttggcaga ccgcctgggt agaaaacctt
      961 tgcttattgt tgcttggctg ttgggtctac tttcaactct tacaatggga agcgcaaaga
     1021 cgctgccctt tttcttaatc ggcttattta catatgggct gacagctttt gtgtcatcac
     1081 cattaagcag ctatgtaact gctgcacgcg gacagttgcc tgttgcaacc gtgctttcac
     1141 tcaccacagc tacattcgga atgggcatgg ttctaggacc cgtaaccagt ggttggatcg
     1201 gcgaacacta tggaatgcgt atgagctttt atgcggctgc cattgtgttt gtattttcca
     1261 acattttcgt gtattttatt ggaagccagc ccatcgacca tcatgacccg tctgctcctc
     1321 cgctaggttt gggcagcaac aaaaaatttg tcaccttaat gttcgttctg gcatttgcga
     1381 ttttctccat gtaccttgcg caaccgctca cgcctaattt tctagaaggc gtgcgcggac
     1441 tttcactcag ccagacgggt cttattttta ccattggcgc actcggaaat tcattgatgg
     1501 cgatcacgtt cagccgtgtg aatccgcggt acggattttt agcagcacag tcgttggtta
     1561 ttctatttgc ggttctgatc tggtatggaa cttctcttcc gttctttgcg ttagggtatt
     1621 tcttgttggg tggttttcga gcggggcgcc ccatggcatt ggcacaagcg cgcgagcttg
     1681 tccatgaatc acagatgggt gtcacttatg gcgtgatgga aactgtcagc gcgatcatca
     1741 caatcattac accgccaatt gcaggctttt tattcgaatt tgaccctttc atcgtttacc
     1801 ccatctccat tgggttattg gtcttgtcta ttcttctaag ctctgtattt ctacctcgaa
     1861 aggaatccca tgcttgagat cgttcaattc actcttggtc ccgcatctac aaatgcctat
     1921 cttgtagcag accccgaaac caaagaagcc gtggtcatag acccctcttg ggatggacat
     1981 ttgatcctcg ccgaagcaca aaaacgcgga tggcgtattg ggcatctttg gtacacacat
     2041 gcccacttcg atcatattgg cggggcagca gctattgccg atgcaatgaa tccgctccca
     2101 catgtggcgc ttcaccccga tgaccatgtc ctctggcgcg agggtggcgg cggcaaaaaa
     2161 ttcggtttgg atatagatcc gggtcccgaa ccgaccattg acctgatcca cggcatgacc
     2221 atgaaactcg gctcgaacga cttccaagtc cgcttcacac ccggtcacac ccgcggacat
     2281 tgtgtattgt atgtagcgaa ggaaagcatc tgcttctgcg gcgatttaat cttcaacgaa
     2341 agtgtcggtc gcaccgatct tccggggagc agtttttcag cgcttgagaa aagtatcc
//
LOCUS       DAOQNJ010000442         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34879_length_2397_cov_6.567037, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000442 DAOQNJ010000000
VERSION     DAOQNJ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34879_length_2397_cov_6.567037"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLG52_11365"
     CDS             <1..380
                     /locus_tag="PLG52_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNB42090.1"
                     /translation="KMLKDAEETCAQLGIPYRVKQLCTGDIGFGATMTYDLELWAPGC
                     NEWLEVSSVSNDTDFQARRANIKFRPSDGGKVKFPHTLNGSGLGLPRALIAVIENYQQ
                     ADGSIVVPEVLRPWMGGIDVIKP"
     gene            521..1048
                     /locus_tag="PLG52_11370"
     CDS             521..1048
                     /locus_tag="PLG52_11370"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HNB42091.1"
                     /translation="MNPADWELFGQVLLEVVTFTVLVFGLLGLLMPVFPGLVVMWIAT
                     LIYAIVQAGLGNMTWVGWTLFVLITLLMIGGNVVDNIIIAKHMRDKDVPWSSILWSFA
                     AGILASLFFTPIIGILASPVGLFLAEWYRLKDRPAAFSSTKTWLTGWGWSVAARIGIG
                     IIMIAFWGLWAWNAG"
     gene            1074..1589
                     /locus_tag="PLG52_11375"
     CDS             1074..1589
                     /locus_tag="PLG52_11375"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HNB42092.1"
                     /translation="MLSEPGQLLLNGLTLTALILSLLSLFFPLFPGLIVMWLALLPYI
                     SIQALTGNMHGSSWLLFAVITVLMLSGSVADIFIIDGHLREKQVPGSSVFWSFMAGII
                     VSLFFTALIGVLASLVGFYMAELSRLKNENKAFDSTKVLLIGWGCSVVLQMSIGVVMI
                     LLWGAMVWTVT"
     gene            1628..1930
                     /locus_tag="PLG52_11380"
     CDS             1628..1930
                     /locus_tag="PLG52_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42093.1"
                     /translation="MDTLQQVLSTFGFLIRAFGFLVLGFGITRFMLDAYYKAVWQVQI
                     AISVGFFLLLIGLTNYSSPASMGTFSIGAGVALLMHFMVKKEEAKEQEHVKETKKK"
     gene            complement(2061..>2397)
                     /locus_tag="PLG52_11385"
     CDS             complement(2061..>2397)
                     /locus_tag="PLG52_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42094.1"
                     /translation="TSEKIKKGLFGGGPGPKLLIQTAILTDRWLILADNVDQNALYIK
                     SMQLRDITVEDYEQSSFYKMIPDTGMNLNGQFTDASELGSMFLPLGKDAAGEKFKSAL
                     IESVQKAKL"
BASE COUNT          532 a          537 c          630 g          698 t
ORIGIN      
        1 agaagatgtt gaaagatgcc gaagagactt gtgcgcagtt gggcattccg taccgcgtga
       61 agcaactctg caccggtgat atcggcttcg gcgcgaccat gacctacgac ctcgagttgt
      121 gggctcccgg ttgtaacgaa tggctcgaag tctcatcggt ttcaaacgat acagactttc
      181 aagcccgccg cgcgaacatc aaattccgcc caagcgatgg cggcaaggtt aaattcccgc
      241 acacacttaa cggctcaggt ttgggcttgc cgcgtgcact tatcgccgtg atagagaatt
      301 accagcaagc cgacggttcc atcgtggtgc ccgaggtcct gcgtccgtgg atgggcggca
      361 tcgacgtcat caagccgtga cggtaaggaa cgaaacgggt taatatccgt acatatataa
      421 acagtagggg cgacccgtcc attgggagcg aatatctcaa tgaaatgtgc agggtcgccc
      481 ttactatttc cggtgccttt cttatcaaag gagaaacgat atgaatcccg ccgattggga
      541 attgttcggg caagtcttgt tggaagtggt tacatttact gttttagtgt ttggtttgtt
      601 aggtttgctc atgcctgtgt tccctgggtt ggtcgtgatg tggatcgcca ctctcatcta
      661 tgccatcgtc caggcagggc ttgggaatat gacctgggtg ggctggaccc tgttcgtgct
      721 cattacctta ttgatgatcg gtggcaatgt agtagataac atcatcatcg ccaaacacat
      781 gcgtgataaa gatgtgccat ggagttccat cctttggtct ttcgctgccg ggattcttgc
      841 cagcttgttc tttactccca ttattgggat ccttgcctca ccagttgggc tttttctagc
      901 ggaatggtac cgcctcaaag accgacctgc agccttttct agcaccaaaa cttggctcac
      961 cggctggggc tggtctgtcg cggcacggat cgggattggc atcatcatga tcgccttctg
     1021 gggactatgg gcttggaacg caggttaagc aggagagtcc tatcattcca tttgtgttga
     1081 gtgagcccgg tcaactgctc ttaaatggac tgactctgac tgcactcatc ctcagtttgc
     1141 taagtttgtt tttcccgctc tttcccggtc tgatcgtgat gtggttggct ttgctgccgt
     1201 atatcagcat tcaagcgttg acggggaaca tgcacgggtc aagttggttg cttttcgccg
     1261 tcatcacggt cttgatgtta agtggcagtg tggcagatat tttcatcatt gatggtcatc
     1321 tgcgcgagaa acaagtgccg ggcagttcgg tattctggtc gtttatggcg gggattatcg
     1381 tcagcttgtt ctttacggct ttgatcggtg tcttggcatc tttggttggt ttttacatgg
     1441 cagagttaag ccgcctgaag aatgagaaca aagcttttga ctcgaccaaa gttttactga
     1501 ttgggtgggg gtgttcggtt gttttgcaaa tgagcattgg tgttgtgatg attttgttgt
     1561 ggggcgcgat ggtatggaca gtgacataac ctagttttgg attatcattc actaaaagga
     1621 gaaacaaatg gacacccttc aacaagtcct ttccacgttt ggctttttaa tacgtgcgtt
     1681 tgggtttcta gtattaggtt ttggcattac ccgcttcatg ctggacgcgt actacaaagc
     1741 cgtttggcag gtgcaaattg ccatttcagt tggtttcttc ctcctactta tcgggctgac
     1801 gaattattcc agccctgctt ctatgggcac attttccatt ggcgcagggg tagcgttgct
     1861 aatgcatttt atggtcaaga aggaagaagc aaaagagcaa gaacacgtca aagagacgaa
     1921 gaaaaaatag gaaagatgaa agaaaaaggc ttgaacgata ccgttcaagc ctttttcttt
     1981 acaagtatgt cacactgagc ggagcgaagg gtctcttaca ttatcgtaga gattcttcgc
     2041 tatcgctcag aatgacatca ttataacttt gccttctgca cggactcgat caacgcggat
     2101 ttgaattttt ctccggcggc atctttccct agcggtaaaa acatcgaacc caattcgctg
     2161 gcatcagtga attgtccgtt taagttcata cccgtatcgg ggatcatttt gtagaatgaa
     2221 ctttgttcgt aatcttccac cgtaatatct cgcagttgca tggatttgat atacagcgca
     2281 ttctggtcga cgttatctgc gaggatgagc cagcggtcgg tgaggatggc tgtttgaatc
     2341 aacagcttcg gacccggtcc gccgccgaac agtccctttt tgattttttc agaggtg
//
LOCUS       DAOQNJ010000443         2396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34903_length_2396_cov_6.385305, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000443 DAOQNJ010000000
VERSION     DAOQNJ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34903_length_2396_cov_6.385305"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLG52_11390"
     CDS             complement(<1..689)
                     /locus_tag="PLG52_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB42095.1"
                     /translation="MSETLVGLVSQYSPSGQERGAVEWLVARMKSLGYDNAFVDEAGN
                     AVGLMGTGTRQVVLLGHIDTVPGEIPVQVVEAAEKSPLLYGRGSVDAKGPLASFVDAV
                     AKVGVKDGWQFVVIGAVEEERDSEGARFVMNQYKPAFAIIGEPNQWDRVSLGYKGSAW
                     ANVTVKRGQVHTASGEETAAEAAIETWLKIKAYVEAYNADKDKVFDKLLLTLRGMESD
                     SNDFEQWAKLKV"
     gene            complement(728..1900)
                     /locus_tag="PLG52_11395"
     CDS             complement(728..1900)
                     /locus_tag="PLG52_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNB42096.1"
                     /translation="MNIVETENKHTSGVYAKQTLTIVRGQGASLFDVDGNEYLDCSSG
                     HGVANLGHAHPKIAEALYKQANTLITLFETFPNDQRAILMEKITALVPGLDRVFFCNS
                     GTEAVEASFKFSRISTGRKNIIAAMRAFHGRTYGSLSATFNKKYREGFEPLLPGVSHV
                     SYNNIEALEKAVNEETAAVILEVVQGEGGVYPASAEYIQAARRICDERGALLIVDEIQ
                     TGFGRTGKMFAVQHYNVTPDLLTCAKSLAGGVPMGAVLIGPNVKNLTPGVHGSTFGGN
                     PLSCAAANAALDVMREEDLPGQAATKGVYLVEKLKKIESPNIREIRGLGLMVGIEMKQ
                     KVTGYIKALQERKIIALNAGLTVIRLLPPLVITYEQIDHLVEVLTEVLVQTTDDGQ"
     gene            complement(1915..>2396)
                     /locus_tag="PLG52_11400"
     CDS             complement(1915..>2396)
                     /locus_tag="PLG52_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42097.1"
                     /translation="VMGHASRFGHAFAKSQIAAGQSLPQSGAVLITVNDMDKSAGLKF
                     ARDMHRMGFRLYATPGTADMCTKAGLPVEVVEKAMDGSTQIVDLIHAGKIHLVLNTPL
                     GPHAHMDGAEIRKAAIAMNVPLLTTLSAAMAAVSAIQALSKKELKYRSLQSHFAAEK"
BASE COUNT          570 a          692 c          523 g          611 t
ORIGIN      
        1 actttcaact ttgcccattg ctcaaaatca ttggaatccg attccatacc gcgcaaagtg
       61 agcaaaagtt tatcgaatac tttatcttta tccgcgttat acgcctcgac ataggctttg
      121 atcttcaacc atgtttcaat cgctgcctct gccgcagtct cttctccact tgccgtatga
      181 acttgtcctc gtttgacagt tacatttgcc caagcacttc ctttatagcc aagcgagact
      241 ctatcccact gattcggctc gccgatgatg gcaaaggctg gcttgtattg gttcataaca
      301 aatcttgcac cttccgagtc acgttcctct tccactgcac caatgaccac aaactgccag
      361 ccatccttga ctcccacttt tgcaactgca tccacaaaac tcgccagcgg acctttcgca
      421 tctaccgagc cgcgcccgta aagtagaggc gacttctcag ccgcctctac aacttggacg
      481 ggaatttctc ctggcacagt gtcaatatgt cccaacaaca ctacttgccg tgttcccgtc
      541 cccattaagc caaccgcatt gcctgcctcg tccacaaagg cattgtcata gcccagtgac
      601 ttcatccgcg ccaccagcca ttcgaccgcg ccgcgttctt gacctgaggg actatattga
      661 gagacaagac caactaaagt ttcactcata gttcctcgac cattgacgat agacgattga
      721 ccatggtcta ttgtccatcg tccgtggtct gcacgagaac ttctgtaaga acttcaacga
      781 ggtgatcaat ttgttcgtag gtaatcacaa gcggaggtaa taaacgaatc actgtgagac
      841 ccgcattcaa agcaatgatt tttctttcct gcaatgcctt gatatacccg gttacctttt
      901 gtttcatttc gatgccgacc atcagtccaa gcccgcgaat ctcacggatg ttgggagatt
      961 cgatcttctt caacttctcg accaaataca ctcccttcgt cgcggcttga ccgggcaaat
     1021 cctcttcgcg catcacatcc aatgccgcat ttgctgccgc acacgataga ggatttccac
     1081 cgaaggtgga gccatgcacg cccggcgtga ggttcttgac gttgggtccg atcaacaccg
     1141 cgcccatggg cacgcctcca gcgagggact tggcacaggt caacagatca ggcgtcacgt
     1201 tatagtgttg aaccgcgaac atcttgcctg tgcgcccaaa gccggtttgg atctcatcca
     1261 cgatcagcaa agcgccgcgt tcgtcacata tccggcgagc ggcttggatg tattccgctg
     1321 aagccgggta cactccgcct tcaccttgca ccacttccaa gatcacagcc gcagtttctt
     1381 cgttgacagc tttctccaat gcttcgatgt tgttgtacga aacatgcgaa acgccgggca
     1441 aaagcggctc gaaaccttcg cgatattttt tgttgaacgt ggcagaaagc gagccatagg
     1501 ttcgcccgtg gaaagcgcgc atggcggcga taatattttt acgtcccgtg gagatgcgcg
     1561 aaaacttgaa cgacgcctcg accgcctccg tgcctgagtt gcagaaaaag actcgatcca
     1621 agccgggaac gagtgctgtg atcttctcca tcagtatggc gcgttgatcg ttcgggaagg
     1681 tctcaaataa cgtaatgagc gtatttgcct gcttgtacaa ggcttcagcg atctttgggt
     1741 gcgcgtgacc taaatttgcc acgccatgcc cggatgaaca atcaagatac tcattgccat
     1801 ctacatcgaa caacgaagct ccctgtccgc ggacgatggt caaggtttgc ttggcataca
     1861 cgcccgatgt gtgtttattc tcagtttcaa caatattcat aataatctcc aatcttattt
     1921 ttctgctgca aagtgacttt gtaaacttct atatttcaat tcttttttgc ttaacgcctg
     1981 aatggcagag actgccgcca ttgccgcaga aagtgtcgta agcagcggaa cgttcatggc
     2041 gatagccgcc ttgcggatct cggcaccatc catatgtgca tgcggaccca acggcgtatt
     2101 caacaccaaa tgtatttttc cagcatggat caaatccacg atctgcgtgg aaccatccat
     2161 cgctttctca accacctcca caggcaagcc agctttcgta cacatatccg ctgtgccggg
     2221 cgtggcgtaa agacgaaagc ccatacggtg catatcacgc gcaaacttca aacctgcgct
     2281 tttatccata tcattcactg tgataagcac agctccgctt tgtggcaggc tttgtccagc
     2341 ggcgatctga gactttgcaa aggcatgccc aaagcgagaa gcatgtccca tcacct
//
LOCUS       DAOQNJ010000444         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34951_length_2394_cov_8.398033, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000444 DAOQNJ010000000
VERSION     DAOQNJ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34951_length_2394_cov_8.398033"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLG52_11405"
     CDS             complement(<1..490)
                     /locus_tag="PLG52_11405"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB42098.1"
                     /translation="MSTREKILDTALTLFNNEGTAAVSTNHIAEAASISPGNLYYHFH
                     NKEEIIRGLFERLFVANDAAFHLSEDKLPTLNDLQTMVKTNYKILWQYRFAHRELAAL
                     LRADEELRTRFLSVRKRGFEGFGQLFQAFTQAGVFKTQSTPEEIQNIAEICWLITEFW
                     LNS"
     gene            complement(590..1276)
                     /locus_tag="PLG52_11410"
     CDS             complement(590..1276)
                     /locus_tag="PLG52_11410"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNB42099.1"
                     /translation="MQIVLAILAVVLAYVIGSVPVGLLIVKIVTGKDIREVESGRTGG
                     TNAMRAAGFWAGFATAMLDIVKGAAGVWVARWLTPDLPVVHMIAPLAAILGHNHSIFL
                     PERDENGRIIRLRGGAGGAPSVGGAMGLYLPSILIVLPLGMLTFFSIGIASVTTMAVA
                     LYSTLAFAYYASQGIIPWAYVLYGVGAEILLVLALRPNIKRLFEGNERVVKYSLNGWL
                     RKRKEEKAAK"
     gene            complement(1346..1762)
                     /locus_tag="PLG52_11415"
     CDS             complement(1346..1762)
                     /locus_tag="PLG52_11415"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNB42100.1"
                     /translation="MRTLSLTNEQLQQMIAHVDSQAPLEACGLLAGRGSSVESVLTVT
                     NQAQSPVRYVMDPIEQLHAFEWIESQGLELLGIFHSHPAGPEVVSSTDIAEAAYDVVY
                     VIVSRINSPWKARGFWIQDGKFDEVTLQIASSSNQP"
     gene            complement(1765..>2394)
                     /locus_tag="PLG52_11420"
     CDS             complement(1765..>2394)
                     /locus_tag="PLG52_11420"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42101.1"
                     /translation="AALTAAGMDWLTRDHPSAVSRATREHLLLPTLTAFVLGTPLALL
                     SNSSAWWWAFLVGAVLLAAVCIAEYISVDPTAPLYGFARAGLTAVAYALFLILVTSLR
                     FSGARMVLVIPSVLIVAGLISLRILHLDGTDRWDFPWAVGIGLVCAQIGAGLHYWPLS
                     PVQFGLAVTGPLYALTMLSNSLTENIPLRRAVVGPIIVAGVAWVTAIFL"
BASE COUNT          667 a          617 c          596 g          514 t
ORIGIN      
        1 ggctgttcag ccaaaattca gtgatgagcc aacagatctc agcaatattt tgaatttctt
       61 ctggggttga ctgtgttttg aaaacgccag cttgggtaaa ggcttgaaag agctgaccaa
      121 acccttcaaa cccacgttta cgaacagaga gaaagcgcgt gcgtagttct tcatctgctc
      181 gcaagagtgc ggcaagttcg cggtgtgcaa aacgatattg ccagaggatc ttgtagttgg
      241 ttttgaccat cgtttgtagg tcattaagag tgggtaattt atcttccgac aaatgaaatg
      301 ccgcatcatt cgctacgaat agacgctcga atagacctcg aataatctct tccttattgt
      361 ggaagtgata gtagagattc cccggactga tgcttgccgc ctcggcgatg tggttggtgc
      421 tgaccgcggc agtcccttcg ttattgaaga gggtgagggc ggtatctaaa atcttttcac
      481 gagtggacat aatgtctcct agagtattta ctctagtatt ttagagtaaa tactctaatc
      541 tgtcaagggt aaaaaaacgc cgaggtcttc aagacctcgg cgttttagct tacttcgctg
      601 ctttttcttc tttacgtttt ctcagccagc cgttgaggct gtattttaca acccgttcat
      661 tgccctcaaa gagacgtttg atattcgggc gtaatgccaa caccaacaga atctctgccc
      721 ctaccccata aaggacatat gcccaaggga tgatgccttg tgaagcataa taagcaaatg
      781 ccaaagtgga atacaacgct accgccatgg ttgtaacaga agcaatccca atactaaaga
      841 aggtcaacat gccaagcggc aggacgatca aaatagatgg aagatacaag cccattgcgc
      901 cacctacaga tggagcgccg cctgcaccgc cacgtaaacg aatgatccgt ccgttctcat
      961 cgcgttcggg cagaaagatc gaatggttat gtccgagaat tgccgccaag ggagcgatca
     1021 tgtgaacaac gggcagatcg ggggtgagcc aacgtgccac ccaaacccct gctgcgcctt
     1081 tcacaatgtc gagcatggca gtggcgaacc cagcccaaaa ccctgccgca cgcatggcgt
     1141 tcgtaccgcc ggtgcgcccg ctttccactt cgcgaatatc ttttccggtc acgatcttta
     1201 cgatcagtaa ccccaccgga accgacccga ttacataagc cagtacaact gccagaatgg
     1261 ctaagacgat ttgcattaag actcctccac gagttgctgt ttaaaattta acatcacaaa
     1321 ttagcagacg taaaaacacg aaaccttagg gttggttgct agatgaggca atctgcaagg
     1381 tcacttcatc aaacttgccg tcttgaatcc aaaatccgcg cgctttccat gggctgttaa
     1441 tgcgggaaac gattacatac accacatcat aagcagcctc agcaatatca gtagaagaaa
     1501 ccacttcggg gccggctggg tgtgaatgga agatgccgag cagttctaac ccttgagact
     1561 cgatccactc gaaggcatgc aattgctcaa tgggatccat cacgtatcgc accggacttt
     1621 gcgcctgatt cgtcaccgtc aacacagact ccacgctgga gccgcgaccc gctaatagac
     1681 cacaagcttc cagtggagct tgcgaatcga catgagcgat catctgttga agttgttcgt
     1741 tggtaaggga aagagtgcgc atgcttacaa aaagatcgca gtgacccaag caacgcctgc
     1801 cacgatgatg ggtcccacta ccgcgcggcg caatgggata ttttctgtaa ggctgttcga
     1861 gagcatggtc aacgcatata gcggaccggt cacagccagc ccgaattgaa ccggcgaaag
     1921 cggccaatag tgcaacccgg cgccaatctg cgcgcagacc aaaccaattc ctaccgccca
     1981 tggaaaatcc caacggtctg tgccatcgag gtgcagaatg cgtaaactga tcaacccggc
     2041 aacgatgagc actgatggaa tcaccagcac catgcgcgca ccggaaaatc ttaacgaagt
     2101 gacaaggatt aagaacaagg cataggcaac agcagtaaga ccggcacgcg caaacccata
     2161 caaaggagcg gttgggtcga cggaaatata ttcggcaata caaacggctg ccagcaacac
     2221 tgcaccaacc aagaatgccc accaccaagc agaactattg gagagaaggg cgagaggggt
     2281 tcctaataca aatgcggtga gggtgggcaa cagcaaatgc tcgcgagtag cgcgactcac
     2341 agcggaggga tggtcgcggg tgagccaatc cattccggcg gcggtaagag ccgc
//
LOCUS       DAOQNJ010000445         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34973_length_2393_cov_11.249358, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000445 DAOQNJ010000000
VERSION     DAOQNJ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34973_length_2393_cov_11.249358"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLG52_11425"
     CDS             <1..806
                     /locus_tag="PLG52_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42102.1"
                     /translation="LAPKAAGLKMYLDSTFGELRLDDMTLWMPHFQKFSKSAPMVMHS
                     ESRTMAAAILFSSIYDHPVHIAHVSLKEEILLIKAAKEKGVKVTCEVCPHHLFLSKDD
                     IPAISHGHPGRGEVRPRLATKEDVHSLWTNVDVIDCFATDHAPHTLAEKDGENPPPGF
                     PGLETLLPLLLQGVQDGRLTIDQIVEKLYTNPKRIFNLPEQPETWVEVDEDVTYEIKA
                     SESFTRCGWTPFEGWKVKGKVRKVVLRGNTAFEDGKVFAEAGSGKNIRG"
     gene            complement(847..2022)
                     /locus_tag="PLG52_11430"
     CDS             complement(847..2022)
                     /locus_tag="PLG52_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNB42103.1"
                     /translation="MSKQTFKWDDPFLLNDQLTDEERMIRDTARKYCQDKLMPRILEA
                     NRHEVFHREIMTEMGNMGFLGSTIPEEYGGAGLNHVDYGLIAREVERVDSGYRSAMSV
                     QSSLVMYPIFTYGTEEQRKKYLPKLASGEWVGCFGLTEPNHGSDPASMEAKAKKVDGG
                     FILHGNKMWITNSPIADVFVVWAKLDGEIRGFILEKGMKGLSAPKIEGKFSLRASATG
                     EIVMDEVFVPAENIFPEVKGLKGPFGCLNKARYGIAWGALGAAEFCWHAARQYTLDRP
                     QFGRPLAANQLMQLKLANMMTEITLGLQGALRVGRLIDEDKVSPEMISLIKRNSCGKA
                     LEIARTSRDMHGGNGISDEYHIIRHVMNLEAVNTYEGTHDIHALILGRAQTGIQAFS"
     gene            complement(2181..>2393)
                     /locus_tag="PLG52_11435"
     CDS             complement(2181..>2393)
                     /locus_tag="PLG52_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HNB42104.1"
                     /translation="YFEQVQNDMNFYWSKDEVLQRLDEKMTSAFNSVFDRSEKEKVYM
                     RDAAYMVAIDRVVKAMELRGWLTGSA"
BASE COUNT          609 a          621 c          572 g          591 t
ORIGIN      
        1 ctctcgcgcc gaaagctgcc gggctaaaga tgtaccttga ctccaccttt ggcgaactgc
       61 gcctcgacga catgaccctg tggatgccgc acttccaaaa attttccaag tctgcgccga
      121 tggtgatgca ctctgaaagc cgaacgatgg cagccgcaat tttgttctct tccatttacg
      181 atcatcccgt tcacattgct catgtttcgt tgaaagaaga aattttgctc atcaaagctg
      241 caaaagaaaa gggcgtcaaa gtcacctgcg aagtttgccc gcatcatttg ttcttatcca
      301 aagacgatat ccccgccatt tcacacggac accccggcag aggcgaagtc cgcccgcgct
      361 tggcaaccaa agaagatgtc cacagcctgt ggacaaatgt ggatgtgatc gattgtttcg
      421 ccactgacca cgctccacac actttggctg aaaaagatgg cgagaatccg ccacccggat
      481 tccccggttt ggagactttg ctgcccttgt tgttacaagg cgtgcaagat ggaagattaa
      541 caatcgacca aattgtggaa aaactttaca ccaaccccaa acgcattttc aatctgccgg
      601 aacagcccga aacctgggtg gaggtggatg aagatgtaac ttatgaaatc aaagccagcg
      661 agagttttac ccgctgcggc tggactccct ttgaaggatg gaaggtaaaa ggaaaagttc
      721 gtaaagtggt gctacgaggg aatactgctt ttgaagatgg caaggttttc gcagaagctg
      781 gctctggcaa gaatataaga ggataaaaaa gaatggacgg gagaaacccg tccattcttt
      841 tttcatctaa gaaaaggctt gtattcctgt ttgcgctctg cctaagatca aggcatgaat
      901 atcgtgtgtg ccttcatagg tgttgaccgc ttccaaattc atcacatgcc gaatgatgtg
      961 atattcatct gagatgccat tgccgccgtg catatcgcgt gacgtgcggg cgatctccaa
     1021 ggctttgccg caggagttgc gtttgataag cgagatcatt tcaggcgaaa ctttgtcttc
     1081 gtcgatgaga cgcccaacac gtaatgcacc ttgtaatccc aacgtaattt ccgtcatcat
     1141 attggcgagt ttcaattgca taagttgatt cgccgccagc ggacgcccga actgcggacg
     1201 gtctaaggtg tattgtcttg cggcatgcca gcagaattcc gccgcaccaa gcgcgcccca
     1261 agcaatgcca tagcgcgcct tgttcaaaca cccgaacgga cctttaagtc ccttcacttc
     1321 ggggaagata ttttctgctg ggacgaatac ttcgtccatc acgatttccc ccgtcgcact
     1381 cgcccgcaga ctgaacttcc cttcgatctt gggcgcggag agtcctttca tgcctttttc
     1441 caagataaag ccgcggatct cgccatcgag ttttgcccaa acgacaaaga catccgcaat
     1501 tggtgagttg gtaatccaca tcttattgcc atgcagaata aaaccgccat ctactttttt
     1561 ggctttggct tccatactgg cggggtcact gccgtgattg ggttcagtca agccgaaaca
     1621 gccaacccat tctccactgg cgagttttgg cagatatttc tttctctgtt cttcggtgcc
     1681 ataagtaaaa atgggataca tcaccagaga tgactgtacg ctcatcgcag aacgatagcc
     1741 gctatccacg cgttccactt cgcgggcgat aagcccatag tccacgtgat tcaagccagc
     1801 gccgccatat tcttcaggaa tggtcgaacc gaggaagccc atgttgccca tctcggtcat
     1861 aatttcgcga tggaaaactt catgtcgatt cgcttcgagg atgcgcggca tgagcttatc
     1921 ctgacaatac ttgcgcgccg tgtcacggat catgcgttct tcgtcggtca attggtcatt
     1981 gagcagaaag ggatcgtccc atttgaaagt ttgtttggac atgaggacct cggggattag
     2041 ggatagagtg attgtatcaa aagcaagggg tagaaaactg tgtcagaaaa cagaagcaag
     2101 catcggctaa gaatgaagcg ttaatcaaaa agaggatgca ccctttcagg cacatcctct
     2161 tatacatcac atcgtactaa ctacgcagaa cccgtcaacc aaccacggag ttccatcgcc
     2221 ttgaccacgc ggtcgatggc gaccatgtaa gccgcatcgc gcatgtaaac cttttccttc
     2281 tcgctgcgat cgaacacgct gttgaaggca gaggtcatct tctcatcgag gcgctgcagg
     2341 acttcatcct tgctccagta gaagttcata tcgttctgaa cctgttcgaa gta
//
LOCUS       DAOQNJ010000446         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_34976_length_2393_cov_7.639435, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000446 DAOQNJ010000000
VERSION     DAOQNJ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34976_length_2393_cov_7.639435"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLG52_11440"
     CDS             <1..482
                     /locus_tag="PLG52_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42105.1"
                     /translation="SVNIHALMTLRDFDYPLDHPSVRKVFQFLDTQKSGDFWVDKWNS
                     SSFYTSSHYVIACAKFKNAIAQPTVDWILKNQRADGSWGTFGSTTEETAYCLQALSIW
                     SRHVSSVPQDVIRRGRTWLLEHYDLPHSPIWIGKGLYTPQWVSCSAILSAMLLTVSY"
     gene            539..2278
                     /locus_tag="PLG52_11445"
     CDS             539..2278
                     /locus_tag="PLG52_11445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB42106.1"
                     /translation="MIANLQNNWERINKFDGFDQDIARRGRLLNVLLLGMSILCLLGI
                     IAIAVVVTMNGTWNKPGNVTTIVAAAVFLIGAIGLGYVNQSSVRLASFLFLALLMVVF
                     IFSDDPAQLANGRSSFVFFIPVAVASLLLTPASSFIFAIAGVAIMIWLANEGNVLPNV
                     STISGEFMFALVSWLSARSLEQALVDLRATNQNLDQLVEQKTRELANTLSRELIVAGR
                     NQAILNSIADGVIVFDERNMAILANPAISRLTNIPVSELVDRSMVDFILRDALSESRD
                     SLQQIFEDPEEETITGVRVNWGSKTLSASVARVNDADDKVIGTVAVFRDITREVELEK
                     MKDTFMGIVSHELRTPLNAILGYAEMLKEGIYGAVNEKQANIAERVMNSTQRLLSIVS
                     DLLDQAQIQSGKLKIRMMSCNPIEMLESLHGVMDRIALEKGIEFRTDIDPAMPTVIMG
                     DPQRLQQVMINLANNAIKFTEKGYVSVKILRMDADHWQIRTTDTGSGIPTDAQEYIFE
                     TFRQVESSAIRQHGGVGLGLSIVKQLVELMKGKITLESEIGKGSTFTVTFPIVINDDS
                     QPTVTDPDRRNYE"
BASE COUNT          590 a          528 c          595 g          680 t
ORIGIN      
        1 ccagtgtaaa tatccatgcg ttaatgacat taagggattt tgactatccg ctcgaccatc
       61 ccagtgtcag aaaagtgttc cagtttttag atactcaaaa atctggagac ttttgggtcg
      121 ataaatggaa ttcttcttca ttctatacct cgtctcatta tgtgattgcc tgtgccaagt
      181 ttaagaatgc cattgctcag ccgacagtgg attggatact aaagaatcaa cgcgcagatg
      241 gctcttgggg tacatttggc tctacaaccg aagaaacagc ctactgcctt caggcacttt
      301 ccatatggtc gagacacgtc agtagcgtac cgcaagatgt gattcgacga ggaagaacct
      361 ggttgcttga gcattacgac ttgccgcatt ccccgatttg gatcggcaag ggactgtaca
      421 ccccgcaatg ggtctcttgt tcagctatct taagtgcaat gttgttaacg gtttcatatt
      481 aactgtttta tgttttaatc attttgtctg tattctctaa gtaaacatgg tggtgcctat
      541 gatcgcgaat ctacaaaaca attgggagcg aattaataag tttgatgggt ttgaccagga
      601 tattgcccgg cgtggtcgcc tgcttaatgt gctcctgttg ggcatgtcca tactttgttt
      661 gctggggatt attgccattg ccgtagtagt aaccatgaat ggtacttgga acaaaccggg
      721 gaatgtaacc accatcgttg cggcggcggt ctttttgatc ggcgccattg ggcttggata
      781 tgtcaatcaa tcttcagtgc gccttgcatc tttcttgttt ttggcattgt tgatggttgt
      841 gtttatcttt agtgatgacc ctgctcagct tgcaaatgga cgttcatcat tcgtgttctt
      901 tatccctgta gcggttgcca gtttgctcct cacccctgcc agcagtttta tattcgcaat
      961 tgcaggcgtg gctattatga tttggttggc gaatgaaggc aatgttttgc caaatgtgtc
     1021 aacaatttct ggcgagttta tgtttgcgct ggtttcatgg ctttcggcgc gctcactgga
     1081 acaagccctg gttgacctgc gtgccaccaa tcaaaacctt gatcagttgg tagagcaaaa
     1141 gactcgcgaa cttgccaata ctctttctcg tgaattgatc gtggctggac gtaaccaggc
     1201 gatcctcaac tcgattgcag atggcgtgat cgttttcgat gaacggaata tggcgatcct
     1261 tgccaaccct gccatcagcc gtctcacaaa tatacccgtt tcagaacttg ttgaccgcag
     1321 tatggtggat tttattttgc gcgatgccct ttctgaatcg cgtgacagct tgcaacagat
     1381 atttgaagac cccgaagaag aaactatcac cggtgttcgt gtcaactggg gcagtaagac
     1441 cctctctgcc agtgtggcgc gtgtgaacga tgccgatgat aaagtgatcg gtactgtggc
     1501 agttttccgt gatattactc gtgaggtaga gcttgaaaag atgaaagata ccttcatggg
     1561 catcgtatct catgaattgc gcaccccgtt gaacgctatt cttggatacg ctgaaatgtt
     1621 gaaagagggt atctacggtg cggtcaacga gaaacaagct aatatcgctg agcgtgtgat
     1681 gaacagcacc caacgtttgc tgagcatcgt tagtgatttg ttagatcaag cacagattca
     1741 atcgggcaag ttgaagatcc gcatgatgtc ctgcaaccct attgagatgt tagagtccct
     1801 gcatggtgtg atggaccgta tcgctttgga aaaggggatt gaattccgca cggatattga
     1861 ccccgccatg ccaacggtca ttatgggaga cccgcaacgg ttgcagcagg taatgatcaa
     1921 ccttgcaaac aatgccatca agtttacaga aaaaggatat gtcagcgtca agatcctgcg
     1981 catggatgca gatcattggc agatccgtac caccgataca ggcagtggca tccctactga
     2041 tgcacaggaa tatatttttg agacctttcg ccaggtagaa agcagtgcta ttcgtcaaca
     2101 tggcggggtt gggcttgggc tttccatcgt caaacagctt gtcgaattga tgaagggaaa
     2161 gatcaccttg gagagtgaaa ttgggaaagg cagtacgttt acggtcactt tcccgatcgt
     2221 aattaatgac gattcgcaac ccaccgttac agatccagat aggagaaact atgaatgacc
     2281 ctctcgcatt gattattgaa gatgatgaag acctcgccga gatctttgga caggcgctta
     2341 ctgctgccgg gtatgccaca gagatcgtcc gtgatgggct tgctgctcaa acc
//
LOCUS       DAOQNJ010000447         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_35041_length_2390_cov_8.620985, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000447 DAOQNJ010000000
VERSION     DAOQNJ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35041_length_2390_cov_8.620985"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1226
                     /locus_tag="PLG52_11450"
     CDS             33..1226
                     /locus_tag="PLG52_11450"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNB42107.1"
                     /translation="MKLNFWLFWRTFYRSFFDSKNTPARLTKKRLIFLTLFYIVWPLG
                     QIMHWFFFWLDDVLFPAYKNQPIEKPLFILGNFRSGSTFLHRLLSRDNETFTSLTTWD
                     IYLTPSVTQKKITQLVAKVDDKYLGSTLHRALFAFDRATLGKVKIHPISFFQPEEDEN
                     IHMHVWDGYFVTFLFPFMDEFPDYIHFDESLSPEHKKRIMTFYKSMLQRHMYATGKKH
                     FVAKNPAFSAKIETLAEFFPDARILYLARNPLDMLPSTVSWINYARGQFTGPNEGYLY
                     IDEILEMTRYWYYHPLQYIDTHPSPRNLIVNYDDLIQRPEAVIRGFYEQFGYPDKPGL
                     PIIIDQAVKETLTFSSDHSYSYEKMGFTRQGIVERYKDIFERFNFETREEEELVPVKR
                     MELER"
     gene            complement(1273..2253)
                     /gene="pyrB"
                     /locus_tag="PLG52_11455"
     CDS             complement(1273..2253)
                     /gene="pyrB"
                     /locus_tag="PLG52_11455"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNB42108.1"
                     /translation="MPTTSTNPHLPFGENKNADWYGKDIISVKQFSRDDLEYVFGVAH
                     EMRGMVERIGTFDLLKGKILANLFYEPSTRTSSSFTAAMERLGGSVIPINEVKYSSVS
                     KGESLPDTIRTLECYADVIVLRHPETGSAAIAAKAAKKPVINAGDGVGEHPTQALLDT
                     FTIMEELGRLDNLNVTMLGDLKYGRTVHSLARLLSQFSNIKLNYVSPDILKMPKEVME
                     EVGAKGVPQAEYSSLEQVLPETDVLYVTRVQKERFEDPADYEKVKGAYVIDPATMKAA
                     KKEMAVMHPLPRVGEISTDFDDDPRAAYFRQMEYGLYVRMALLAMVLGKA"
BASE COUNT          627 a          606 c          498 g          659 t
ORIGIN      
        1 agcacgtatc acacttaagg atcccgccct taatgaaatt gaacttctgg ctattctggc
       61 gaaccttcta ccgttcattc tttgactcaa aaaatacccc agcccgtctc accaagaaac
      121 gactgatatt tttgaccctt ttctacatcg tctggcccct tgggcagatt atgcattggt
      181 ttttcttctg gctggatgac gtcctctttc ctgcttataa aaatcaaccc attgagaagc
      241 cgctctttat tttgggtaac ttccgcagtg gctccacgtt cttacaccgt ttgctttcac
      301 gtgacaatga aacctttacc agcctgacca cgtgggatat ctatctcaca ccgtcggtga
      361 cccagaaaaa aatcacgcaa cttgttgcta aagtggatga taaatattta ggaagcacat
      421 tacaccgtgc cctatttgca ttcgaccgtg ccacgcttgg caaggtaaag atacacccca
      481 tctcattctt ccaacccgaa gaagatgaaa acattcatat gcatgtatgg gacggatact
      541 ttgtcacctt cctcttccca tttatggatg aattccccga ttacattcac tttgatgaat
      601 cgctcagccc tgaacataaa aaacgtatca tgacgtttta caaatccatg ttgcagcgcc
      661 atatgtatgc aacgggcaag aaacattttg tggcgaagaa cccagcattc agtgccaaga
      721 tcgaaacctt ggctgaattc ttccccgatg cacgcattct ctatctggct cgcaacccgc
      781 tcgatatgct tccatcaact gtttcatgga ttaactatgc acgtggtcaa tttacaggtc
      841 ccaatgaagg ctacctgtat attgacgaaa tcctcgagat gacgcgctac tggtactatc
      901 acccccttca atatatcgat acgcatcctt ctccccgcaa cctgatcgtc aactacgacg
      961 atttgatcca acgcccagaa gccgtcattc gcggattcta tgagcaattt ggttatcccg
     1021 ataaacccgg tctgcccatc atcattgacc aagccgttaa agagacattg accttcagca
     1081 gcgaccattc atattcctat gaaaagatgg gctttacccg tcaaggaatc gtagaaagat
     1141 acaaagatat cttcgaacgc ttcaactttg aaacgcgcga agaggaagaa cttgttccag
     1201 tcaaacgcat ggaattagaa cgataaaaca aaaaacgcgg acaaaaatgt ccgcgttttt
     1261 atttaagctc gtttaggctt ttccaagcac catggcaagc aatgccattc tcacgtacaa
     1321 accatattcc atctggcgga agtaagcagc gcggggatca tcatcaaaat ctgttgagat
     1381 ttcacccaca cgcggaaggg gatgcatgac cgccatttct ttctttgcag ctttcatagt
     1441 agctggatca atcacataag cgccctttac tttttcataa tcagcggggt cttcaaagcg
     1501 ttctttctga acacgggtga catagagcac atccgtttca ggcaagactt gctccaaaga
     1561 agaatattcc gcttgcggca cgcccttagc gcccacttct tccattactt ctttcggcat
     1621 tttcaaaata tctggcgaga cgtaattgag cttgatgttg ctgaactgcg agagcaatct
     1681 cgccagcgag tggacagtgc gcccatattt caaatcaccg agcatagtga cgttcaagtt
     1741 atcaaggcga ccaagttctt ccataatggt gaatgtatcg agcaaggctt gagtcgggtg
     1801 ctcaccgacc ccatcgcctg cattgatgac cggtttcttt gctgcttttg ccgcaatcgc
     1861 cgcggacccg gtttcggggt gacgcagaac gatcacatcg gcataacatt ccaaggtgcg
     1921 gatggtatcg ggcaagcttt cacctttgga tactgatgag tatttcactt cattgatcgg
     1981 aatcactgac ccacccaaac gctccatggc agccgtgaat gaagatgaag tacgtgtgga
     2041 tggttcgtag aagagattag caaggatttt ccctttgagt aagtcaaagg ttccaatgcg
     2101 ctcgaccatt ccgcgcattt catgcgccac gccaaagaca tattccaagt catcacggct
     2161 aaattgcttg acggaaataa tgtccttgcc gtaccaatcg gcgttcttgt tctcgccgaa
     2221 aggcaaatgg gggttcgtgg aagttgttgg catttcttag tctcctggtt ctgataattg
     2281 gttatcaatt cgatatatga gttgggcata tatcggtatt tacttgtctt catgcccatg
     2341 ccgatggtga atatctggca tatggtcatg gtcatgttct tcttcagggt
//
LOCUS       DAOQNJ010000448         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_35071_length_2389_cov_8.916881, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000448 DAOQNJ010000000
VERSION     DAOQNJ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35071_length_2389_cov_8.916881"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLG52_11460"
     CDS             complement(<1..364)
                     /locus_tag="PLG52_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNB42109.1"
                     /translation="MNLDDLDLFKQIDKQNMRAQIDGLPDQLQSAWDFAQTLQLPTFG
                     DIHNIILSAMGDSGSAAELVVASVFSSLSLPVSVHRGYGLPASATDKTLVVCLSHSGN
                     SEEVLDAFESALKNGCKII"
     gene            complement(419..1891)
                     /locus_tag="PLG52_11465"
     CDS             complement(419..1891)
                     /locus_tag="PLG52_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42110.1"
                     /translation="MAEEKKKDEEKKDEKATPKDNIVTTKHSVKIGGKTIKYTVTTGT
                     MVLKEEVNEKDSEIEKPRAQIFFTAYTMDGVRDKAKRPITFSFNGGPGSSSVWLHLGV
                     LGPRRVVMTNDGEMPKPPFKLTDNEFSVLDETDLVFIDPMSTGYSRPVEGEKSKDFHT
                     FQKDIASVGDFIRLYTTRYNRWLSPKFLAGESYGTTRAAGLSGYLADRHGMLLNGLML
                     VSSVLDFTTVDFNHNNDLPYILFVPGYTATAWYHGKLDKKKPLKTWLKESEEFALGEY
                     ATALMKDGALSKEERRKVAEKLARLTGISADFVERGNLRIHTFHFFKELLRDEGKTVG
                     RLDSRYTGIDRLGVTETFEYDPLFAQVNGPYTAAFNDYIRTELKFETDSTYEILSDKV
                     WRQWSYSYFENQYVNVAETLRAAMTFNKYLKVFVANGYYDLGTPYFATEYTFNHLALD
                     ESLRGNISMGYYEAGHMMYVHMPSLKQMKKDLSKFIKDAS"
     gene            complement(1903..>2389)
                     /locus_tag="PLG52_11470"
     CDS             complement(1903..>2389)
                     /locus_tag="PLG52_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42111.1"
                     /translation="RWLLKHYETKFSFTKGLGVVAWISAYAFALRFNIIKMFELYNAL
                     PTEPPNCYIATAAAKGHPRFVGSREVTLASGKSMRVNRQLQRLKALEIVWMGVSAPSH
                     RMMRQVYDVIGKRLAKQIQNPLLADIAYLILVPVELLSFFVLKSIVPEIQTLSERLYR
                     S"
BASE COUNT          569 a          548 c          637 g          635 t
ORIGIN      
        1 cgatgatctt gcatccgttt ttcagtgcgg attcaaacgc atccaacact tcttcagaat
       61 tgccggagtg cgagaggcaa acaaccagag tcttgtctgt ggcagaggcg ggcaatccgt
      121 agccgcggtg aacgcttacg ggtagtgaaa ggcttgagaa aacagaagca actaccagtt
      181 cggcagccga gccggaatct cccattgcgc tgaggatgat gttgtggatg tcaccaaaag
      241 ttggcagctg cagagtctgg gcaaaatccc atgcgctttg tagttgatcg ggcaagccgt
      301 caatttgggc gcgcatgttt tgtttatcga tttgtttgaa gaggtcgagg tcgtctaggt
      361 tcatggttta tttacaatga gacctgtcag gtttttaaaa cctgacaggt ctttttaatt
      421 agctcgcatc tttgatgaac ttgctcaaat ccttcttcat ctgcttgagc gaaggcatgt
      481 ggacgtacat catgtgcccg gcttcgtaat agcccatgct gatattgccg cgcagggact
      541 cgtcgagggc gagatgattg aaggtgtatt cggtggcgaa gtaaggcgtg ccaagatcgt
      601 agtagccgtt agcgacgaag accttgagat atttattgaa ggtcatcgcg gcgcggagcg
      661 tttcagcgac gttgacatat tgattctcaa aataactgta cgaccattgc cgccaaactt
      721 tatcgctgag gatctcatag gtcgagtcgg tttcgaattt gagctcggtg cggatgtaat
      781 cgttgaatgc cgccgtgtaa ggaccattga cctgtgcaaa gagcgggtcg tattcaaaag
      841 tttcggtcac accgaggcgg tcgatgccgg tgtaacggct gtcgaggcga ccaacggttt
      901 tgccttcatc gcgcaaaagt tccttgaaga agtggaaggt gtgaatgcgc aagttgccgc
      961 gttcgacgaa gtctgctgag atgccagtga ggcgggcgag tttctcagct acttttcttc
     1021 tttcttcttt agagagcgca ccatctttca tcaaagcggt agcatattca cccaaagcaa
     1081 attcttccga ttccttgagc caagtcttga gcggtttctt tttatccaac ttgccgtgat
     1141 accacgcggt ggcggtgtaa cccggaacaa agagaatgta aggcaggtcg ttgttgtgat
     1201 tgaaatcaac cgtggtgaaa tcaagcacag atgaaacgag catcagtcca ttaagcaaca
     1261 tgccatgacg gtctgcaaga tagccggaga gtcccgccgc gcgtgtggtg ccataggatt
     1321 cgcccgcgag gaatttcggt gagagccagc ggttgtaacg tgtggtgtag agccgaatga
     1381 aatcacctac cgaggcgata tctttttgga aggtatgaaa atctttcgac ttctcgcctt
     1441 ccacggggcg gctgtagccg gtgctcatcg ggtcaatgaa aaccaggtcg gtttcatcga
     1501 gcacagaaaa ttcgttgtct gttagtttga agggcggctt gggcatctcg ccgtcattgg
     1561 tcatcaccac acggcgcgga ccgagcacac cgaggtgcaa ccaaacggat gaagagccgg
     1621 gtccgccatt gaaggagaaa gtgatcgggc gcttggcttt atcgcgcaca ccgtccattg
     1681 tgtaggcagt gaaaaaaatc tgtgcgcgcg gtttttcgat ctcactatct ttttcgttca
     1741 cttcctcttt caaaaccatc gttccggttg tgacggtgta cttgatggtc ttgccgccga
     1801 tcttcaccga atgtttggtg gtgacaatgt tatcttttgg ggttgctttt tcgtctttct
     1861 tctcttcgtc tttctttttt tcttcagcca tgtgagtctc ctttatgagc gatataaacg
     1921 ctcagacaaa gtttgaattt cagggacgat tgattttagt acaaagaacg acaataattc
     1981 aacaggaacc aaaataagat atgcaatatc agccagtagc ggattttgga tctgcttcgc
     2041 cagccgtttg ccaatgacat catacacctg ccgcatcatt ctatgacttg gagcggatac
     2101 gcccatccaa acaatttcca acgctttgag cctctgcaac tgacgattga cccgcatgga
     2161 ttttccgctt gccaatgtca cctctcgcga accgacgaag cgtggatgtc ccttcgcggc
     2221 ggcggtggcg atgtaacaat tgggcggttc ggtaggcaag gcgttataca actcgaacat
     2281 tttgatgatg ttgaagcgca gggcaaaggc atacgctgaa atccaagcga cgacgccaag
     2341 tccttttgtg aaagaaaact tggtttcgta gtgtttcaaa agccaacga
//
LOCUS       DAOQNJ010000449         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_35072_length_2389_cov_8.499572, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000449 DAOQNJ010000000
VERSION     DAOQNJ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35072_length_2389_cov_8.499572"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2355
                     /locus_tag="PLG52_11475"
     CDS             <1..2355
                     /locus_tag="PLG52_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB42112.1"
                     /translation="ALHGDSTFGDVLRYLRERAHLSQRELALLVGYHFSYISYLEKNT
                     RSIDEASLLGRFVPALGLEDEPEWVARLVELSKNKNGETPTVRRLSEAATGEENADSL
                     PTSLTAMIGREYESARLQKMILDPEIRLISIIGPPGVGKTCLAIAVAHKVFSAFEQGV
                     IFVDLTTTDNPQMFLPTLANALSVQESSSVSIEDAIKSSLAKKNVLLVLDNFEQIVDA
                     SPKIIPLLTAGNAVKILVTSREALRLRGEYEFHLAPLPVPSEYEKMSTEQLRNFASIA
                     LFVERAQAVRPEFSLDEKNASQIAEICSRLDGLPLAVELAAARIGVLSLPAMLNQFPR
                     RFDWLTQGARDLPEWRQTLLGAVEWSYNLLSEKERVLLCRLSIFSGGWTLEAAEEVCS
                     DDDQCLRSEIFSLLIQLTDKSLVVPETEADRFHFLETLREFAREKLKADGDLERMRER
                     HCAYFLKFAQTARPHLVQGKDQLLWLNRMEIEYKNLREVLEWAVEDASRVTIAMDFAM
                     AVHGLWLTRSRINEARYWLAKIFALDSSPGKTRVSLLQFASSFACAQGDFSTAHLLEK
                     EAMEISRILDDEEGIYSSMDGMAMIAGMQGDYKKAAELLEEALRYYRNVPYTPRLTAT
                     LNNLAISMRRLGDLDRAMQLITEAIGITKKVGNQKSLAHALNSLAELQNEMGNIETAL
                     QIHHEAILLRLQLGDRKGLAFSFDSAAECMEKLGNYRLAVQLWGASSRIRDELGLSIP
                     PATLDEYNTLLARARTKLGNSEFESVWTEGRSMQQDRLLAIVFG"
BASE COUNT          569 a          535 c          617 g          668 t
ORIGIN      
        1 gccctgcatg gggattctac atttggcgat gtcctacgat atctacggga acgtgctcat
       61 ctctcccagc gtgaactggc tttgttggtg ggatatcact tcagctacat cagctatctt
      121 gaaaaaaata cacgctccat tgacgaagcg tctttattgg gcaggtttgt gccagcttta
      181 ggccttgagg acgagccgga gtgggtcgcc cgtttggtcg aattatctaa aaataaaaat
      241 ggagaaacgc caaccgtgcg gcgtttatct gaggcggcta ccggggagga aaatgctgat
      301 tcgttgccga ccagcctcac ggcgatgatc ggccgcgagt acgaatccgc ccgtttacaa
      361 aaaatgattc ttgatcctga aatccgcctg atcagcatta ttggtccgcc tggagtaggg
      421 aagacatgtc ttgcgattgc cgttgcgcac aaggtttttt ctgcgtttga gcaaggtgtg
      481 atttttgtgg atttgacgac aacggataac ccacaaatgt ttcttccaac attggcgaat
      541 gccctgagtg ttcaagagtc ttcatctgtt tccattgaag atgcaattaa atcttcattg
      601 gctaaaaaga atgtattact ggtgctggat aattttgaac aaattgtcga tgcctccccc
      661 aaaatcatcc cgctgttaac cgcggggaat gcggtaaaga ttcttgttac cagtcgtgaa
      721 gcgcttcgcc tgcgtggtga atacgagttt catcttgccc cgctccctgt tccgtctgag
      781 tatgaaaaaa tgtcaactga gcagttgcgg aattttgcgt ccattgcttt gttcgttgag
      841 cgtgcccaag ctgtgcgccc tgaattttct ttagatgaaa aaaacgcttc gcaaatcgcc
      901 gaaatctgtt cccgcctcga cggtttgcca ctggctgtgg aattagctgc ggcacggatt
      961 ggggttttga gcttacccgc catgctgaac cagtttccgc gccgttttga ctggcttacg
     1021 cagggcgccc gtgatttacc tgaatggcgc cagacattgc tgggggcagt ggagtggagt
     1081 tacaacctcc tctctgaaaa agaacgcgtt ttgctttgca ggctttccat tttttccggc
     1141 ggctggacct tggaagccgc tgaggaggtt tgcagtgacg atgaccaatg cctgcgctcg
     1201 gaaattttca gccttctcat tcaattgaca gataagtcac ttgttgttcc agaaacggaa
     1261 gcagatcgtt ttcactttct ggaaaccttg cgtgagttcg cccgcgaaaa attaaaagcg
     1321 gatggcgatt tggaacgtat gcgagaaagg cattgtgctt attttttgaa atttgctcaa
     1381 actgcaaggc cgcatcttgt tcaaggcaaa gatcaactgc tttggctgaa tcgaatggaa
     1441 attgaatata aaaacctgcg tgaggttttg gagtgggctg tcgaagacgc atctcgggtg
     1501 acgatagcca tggattttgc catggcagtc catggacttt ggcttacccg tagccgcatc
     1561 aatgaggcgc gttattggct tgctaaaata tttgccctcg attcttcgcc cggtaaaaca
     1621 cgcgtgagcc ttttgcagtt tgcgagtagt tttgcatgtg cacagggtga cttttccacc
     1681 gcccacttgc ttgaaaaaga ggccatggaa atttcccgta tccttgatga cgaagaagga
     1741 atttattctt ccatggatgg tatggcgatg atcgctggaa tgcagggaga ttacaaaaaa
     1801 gctgcggaat tgctggaaga agcccttcgg tattaccgta atgtacctta cactccgcgc
     1861 cttacagcga cattgaataa tcttgcaatt tccatgcgcc gacttggcga ccttgaccgt
     1921 gccatgcagt taattacaga agccattggc atcacaaaaa aggttggaaa tcaaaagtca
     1981 ctggcgcatg ctctaaacag ccttgccgaa ctccaaaatg aaatggggaa tattgaaacg
     2041 gcgctccaga tacaccatga ggcgattttg cttcgtcttc aacttggtga ccgaaaaggc
     2101 ttggcatttt cctttgattc cgccgctgaa tgtatggaaa agcttggtaa ttatcgtctg
     2161 gctgtgcagt tgtggggcgc ttcttccagg atccgtgatg aattggggtt gagtattcct
     2221 ccagctactc ttgatgaata caatacattg ctggcgcggg cacgtacaaa actgggtaat
     2281 tctgaatttg aatctgtctg gaccgagggg cggtccatgc agcaggatcg cttgcttgcc
     2341 attgtatttg gttaggtgaa ttgatttgcg gggtgtttat tgtgccaaa
//
LOCUS       DAOQNJ010000450         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_35177_length_2385_cov_11.385837, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000450 DAOQNJ010000000
VERSION     DAOQNJ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35177_length_2385_cov_11.385837"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1718)
                     /locus_tag="PLG52_11480"
     CDS             complement(63..1718)
                     /locus_tag="PLG52_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNB42113.1"
                     /translation="MAMPLYLECNLCNHQQAYIPFVPAICEKCESQWVEARYDYESFK
                     REILRGIPNRPNNLWRFQDVLPLKNPSALDLYPAGGTPLWLSQRFAPDLGHGSVYIKD
                     ERYSPTSSFKDRQAAVAVAAMNENGIREAVIASTGNAAVAYAAACARAGIKLWVFMTS
                     RVPQEKLREAALFGAEVIRVSGNYDQTKQIASQFAQRKNLLLDRGASSVPARESMKTI
                     AYEIVEQLGWRAPDWYIQAVSGGLGPLGVYQGFKEMFNMGLIKKVPKLAIIQSAGCAP
                     MVNAFKEGKDVATPVIPDTRITILSTGDPGKAYTYLWNLTQQYGGVMESVTDLEAYEA
                     MKTLAKSEGMAVEPATSVAFAGFQKLSGQGIISKDELVAINCTGHTFPVEKHVLGDQW
                     AVDVHLSKDQSPAPHEGLQAALENLDEKTTTILLVDDNSDDALLIRRLLEGRKNYRMY
                     HAKDGWEGLSVARQKLPDLIVSDLTMPGIDGFGLVEELKLDPRTKDIPVVVVSAKDIT
                     KEERLRLNGQIEALYQKGSLPTRKFVDKVIGVIEDKFEEKGKE"
     gene            complement(1719..>2385)
                     /locus_tag="PLG52_11485"
     CDS             complement(1719..>2385)
                     /locus_tag="PLG52_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB42114.1"
                     /translation="LGFADVMLEGLDGPLTSTMDNDLRLIQKNGQHLLHLINDVLDMA
                     KIESGRMSLHADTFNVNNLLEEVTSITSSLASDKNIALYIEPESDREVEIYADSTRIR
                     QVMINLVNNSIKFTEKGSVALNVSRMDDARVLITVKDNGLGIPTDQLETIFLEFTQVD
                     TTTTRKVGGTGLGLPISRRLVEMHGGRLWAESSGIDGEGSTFFVELPLETRVTEIIEK
                     REK"
BASE COUNT          545 a          590 c          599 g          651 t
ORIGIN      
        1 accaacatca tattatccgg attatcttct atgcagagga tgatgtttcc cataagttct
       61 ccttattctt tgcctttttc ttcaaattta tcttcaatga ccccgataac tttatccacg
      121 aattttcgag tggggaggga tcctttttga tatagtgcct caatttgacc gttgaggcga
      181 aggcgttctt cttttgttat atcttttgcg ctaacgacca caacaggaat atcttttgta
      241 cgtgggtcta gctttaattc ttctaccaaa ccaaacccat caatacctgg catggtcagg
      301 tcgcttacga tcaggtctgg caatttttgt cgtgcaacag agagaccttc ccagccatct
      361 ttggcgtgat acatgcggta gttcttccgc ccttccagca aacggcggat aagcagtgca
      421 tcatctgagt tatcgtccac taataagata gttgtggtct tttcatccaa gttttcgagc
      481 gcggcttgca acccttcatg tggagcgggg gactggtctt tgctaagatg cacatccact
      541 gcccattgat ctcccaagac gtgtttttct acggggaagg tatgccctgt gcagttgatg
      601 gcaacaagct catctttgct gatgataccc tgcccagata acttttgaaa acccgcaaat
      661 gctacagaag tcgcgggttc cactgccatc ccttcgcttt ttgcgagggt tttcatggct
      721 tcgtatgcct caaggtctgt aacggactcc atcacgccgc cgtattgctg agtgaggttc
      781 cacaagtagg tgtatgcttt accggggtcg cctgtggata ggatggtgat gcgtgtatca
      841 ggaatgactg gcgtggcaac atccttccct tccttgaagg cgttgaccat gggagcgcaa
      901 cccgcagact ggatgatggc aagtttaggc acctttttga tcagccccat gttgaacatt
      961 tctttgaaac cttggtaaac gcctaacggt cccaacccgc cgctaacggc ttgaatatac
     1021 caatcggggg cacgccagcc gagctgttct acgatctcat aggcaatggt cttcatcgat
     1081 tcgcgggcag gcacagaact tgcgccgcgg tcgagcaaca gatttttgcg ttgcgcgaac
     1141 tgactggcga tctgcttggt ctggtcgtag ttgccgctaa cgcggatcac ttctgcacca
     1201 aagagggcag cttctcgcaa tttttcctgt ggcacacggc tggtcatgaa aacccatagt
     1261 ttgatgcctg cacgtgcaca agcggctgca taggctacag cagcattccc tgtggaggca
     1321 atgaccgctt ctcgaatgcc attttcattc atggcggcca ctgccaccgc ggcttgacga
     1381 tctttgaaag aagaggtcgg gctgtagcgt tcatctttga tgtaaacaga accatgcccg
     1441 aggtccggag cgaagcgctg tgagagccag agtggagttc cacctgcggg gtataggtca
     1501 agcgcagatg gattctttaa tggcaagaca tcctgaaagc gccacaagtt atttgggcgg
     1561 ttgggaatac cgcgcaagat ctcacgtttg aacgactcgt agtcgtaacg agcttcgacc
     1621 cattgagatt cgcatttttc acaaatggca ggcacgaatg gtatgtaggc ttgttgatgg
     1681 ttgcaaagat tgcattcgag gtaaagcggc attgccattt atttttctct tttctcaatg
     1741 atttctgtaa cgcgagtttc aaggggcaat tctacaaaga atgtagagcc ttcaccatca
     1801 atgccggaac tttctgccca gagacggcca ccatgcattt cgacaaggcg gcggctgatc
     1861 ggcagaccca accctgttcc accgaccttg cgggtggtgg tggtgtcgac ctgtgtgaat
     1921 tccaagaaga tggtttcaag ctggtcggtt ggaatgccaa gcccattatc tttgacggta
     1981 atcagaactc gggcatcatc cattcgcgag acattcagag cgacgcttcc cttctcggtg
     2041 aacttgatgg agttgttcac aaggttgatc attacctgac ggatacgagt gctatcagca
     2101 tagatctcga cctcacggtc tgattccggt tcaatataca aggcgatgtt cttatcgctt
     2161 gccagagatg atgtgatgct ggtaacttct tcgagcaggt tgttgacatt gaacgtatcg
     2221 gcatgcaagc tcatgcgacc agactcgatc ttcgccatgt cgagcacatc gttgataagg
     2281 tgcaacagat gttgaccatt cttttggatc aagcgcaggt cgttatccat tgtagaggtc
     2341 agcggaccgt ctaacccttc gagcatcaca tcggcgaagc caagg
//
LOCUS       DAOQNJ010000451         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_35203_length_2384_cov_10.118935, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000451 DAOQNJ010000000
VERSION     DAOQNJ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35203_length_2384_cov_10.118935"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLG52_11490"
     CDS             <1..236
                     /locus_tag="PLG52_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HNB42115.1"
                     /translation="HLSIIMRALTSKDENEIVYCLSLLKHTHAGTGFMHESFDKDNPD
                     NFTRTWFAWANSLFGELILKIHNERPRILKMFL"
     gene            292..567
                     /locus_tag="PLG52_11495"
     CDS             292..567
                     /locus_tag="PLG52_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42116.1"
                     /translation="MDNDLETMTKEELIAEVRKLRTGIRNHRDATEHELCWYHPDLWN
                     LLPEKVSPEILVPEWSKFMNGCIHYRKSLDEQAPNAPRVDIDFEDEK"
     gene            623..1420
                     /locus_tag="PLG52_11500"
     CDS             623..1420
                     /locus_tag="PLG52_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNB42117.1"
                     /translation="MTLEEIPLTGGNVNSNVVRIGNTVRRAMTKVSPTVHQLLLHLEA
                     KGFVGAPRFLGIDEQQREILSFIEGETTFSEELWQTDAPLVAAAQLLKNYHDATLDFP
                     QKDGLVWGITSPDPARHEVICHNDFAPYNFIFQAQSPYAVIDFDLVGPGPRLRDVAYA
                     AYWLTPLSFHSADLLPFTEADVKNDSRRLHLFCETYGIRASEALFDMVYEVLCFMGDK
                     TKMEEILGDEIAAKLEREGHVKHWQAEAQAFLENRNRFAKNLSQPDQ"
     gene            1431..1733
                     /locus_tag="PLG52_11505"
     CDS             1431..1733
                     /locus_tag="PLG52_11505"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNB42118.1"
                     /translation="MTTDPTFRMTVQDIFFIKGRGTVVTGRVESGIITVGDEIRIQGK
                     SANKTAIVTGVEVLRKVTTQAKAGDNIGLLFKDITKEDVQVGDILTGSDLDFTWKP"
     gene            1770..2381
                     /locus_tag="PLG52_11510"
     CDS             1770..2381
                     /locus_tag="PLG52_11510"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNB42119.1"
                     /translation="MKEEIHITFLRHGRSRADDENVHEGRYDSPLTDVGRSQVQARVQ
                     DFLSRQFHFDAAISSPLQRAHETATIIGQALSLPIETDADWMEMDNGPLAGMPWDEAE
                     KQYPKPAFRNPYESFCGSGESDWELYTRGAKAVEKIIRRGPGSYLVVAHGGILNSALR
                     TIIGTQPSINQQGITFGFGDTGYARFSYRPAQHLWYLLEFKME"
BASE COUNT          698 a          550 c          532 g          604 t
ORIGIN      
        1 ggcacctgag cattatcatg cgcgctctaa caagcaagga tgaaaatgag atcgtttatt
       61 gcctgtctct gctcaaacac acacatgccg gaacgggttt catgcatgaa tcttttgaca
      121 aagacaatcc agataatttc acccgcacgt ggttcgcttg ggcaaactcg ttgtttggtg
      181 aattgattct aaagatacac aacgaacgtc cgcgcatttt gaagatgttc ttgtaacaac
      241 gcaaaagaca tcaaattcct ttttcacaag gaagccgttt caggagatac catggataac
      301 gatctagaaa caatgacgaa agaagaatta atagctgaag ttaggaaact tcgaaccggt
      361 attcgaaatc atcgtgatgc aacagagcat gaattgtgtt ggtatcatcc agacctttgg
      421 aatcttctcc cagaaaaagt atcgccagag atactggttc cagaatggtc aaagttcatg
      481 aatggctgca ttcactatag aaaaagctta gacgaacaag ccccaaatgc gccccgtgtc
      541 gatatagatt ttgaagatga aaaatagcct tcagtatctt tcgagtaatt gtgtacagta
      601 aacatatcac acaaaaattc aaatgacgct tgaagaaatc cccttaactg gcggcaatgt
      661 caattccaat gtggtgcgca ttgggaacac tgtgcggcgt gcaatgacca aggtcagccc
      721 aacagtacac caactgcttt tacatttgga agccaaaggc tttgtggggg ctccacgatt
      781 tttaggcatt gatgagcaac aacgcgaaat attatccttt atagaagggg aaactacgtt
      841 ttcagaagaa ctttggcaaa cggacgctcc gctggttgcc gccgctcaac tcctgaagaa
      901 ttatcatgac gccaccttgg attttcccca aaaagatggt ctggtgtggg gcatcacatc
      961 tcctgaccct gctcggcatg aagtcatctg tcataacgac tttgcccctt ataatttcat
     1021 cttccaagcc caatcgcctt atgcagtgat tgattttgat ctggtaggtc cgggtcctcg
     1081 gttgcgagat gtggcatatg ccgcttattg gcttacaccc ttatcatttc atagtgctga
     1141 cctgcttccc tttacagaag cggacgtaaa aaatgacagt cgccgtcttc acctgttttg
     1201 cgagacctat ggcatccgtg ccagcgaggc tctttttgat atggtctatg aggttttatg
     1261 ttttatgggt gacaaaacga aaatggaaga aatcctaggg gatgaaattg ccgcaaaact
     1321 tgaacgagaa ggacatgtaa aacactggca agcagaagcc caagcttttc tagaaaatcg
     1381 aaatcgcttc gctaaaaatc tgtctcagcc cgatcaataa aggattcccc atgaccacag
     1441 accctacttt tcgcatgacc gtacaagata tcttctttat aaaaggacgc ggaaccgttg
     1501 taactggtcg cgttgaaagc ggaataatca cggtcggaga tgaaattcga atccaaggga
     1561 aaagcgccaa caaaacggct atagtaacgg gggtagaagt ccttcgcaaa gtcacaacac
     1621 aggcaaaagc tggtgacaat atcggactac tgttcaaaga catcacgaaa gaagatgttc
     1681 aagttgggga tatcctaaca gggtctgatt tggatttcac ctggaagcca taaacgacct
     1741 tatccgatct tgttattttg ggagtactca tgaaagaaga aatccatatc accttcctgc
     1801 gccacgggcg gtctcgcgcc gatgacgaaa acgttcacga aggccggtac gactcacctc
     1861 tcactgatgt agggcgttcc caagtccaag cccgcgtgca agattttctc tcccgacaat
     1921 ttcattttga tgcggctatt tcaagcccgt tgcaacgagc acatgaaacc gccacgatca
     1981 ttgggcaagc gttaagtctc cccattgaaa cggatgccga ttggatggaa atggataatg
     2041 gtcccttagc tggcatgcct tgggacgaag ccgaaaaaca atatcccaaa cctgcatttc
     2101 gaaatccata tgaatcgttt tgcggttcag gagaaagcga ctgggagctt tacactcgcg
     2161 gagccaaagc tgtagaaaaa atcattcggc gcggacccgg aagttattta gtcgtcgccc
     2221 atggtggcat tctcaattct gctttacgga caattatcgg cacacaacca tctatcaacc
     2281 aacagggcat cacatttggt tttggtgata ctgggtacgc ccgctttagt tatcgtcctg
     2341 ctcaacatct atggtatctc ctcgaattca aaatggaata aata
//
LOCUS       DAOQNJ010000452         2373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_35470_length_2373_cov_5.490509, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000452 DAOQNJ010000000
VERSION     DAOQNJ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35470_length_2373_cov_5.490509"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLG52_11515"
     CDS             <1..790
                     /locus_tag="PLG52_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB42120.1"
                     /translation="ASSGIPKMMRENIITLPFNDFEGFERVMRSYGEQIAAVISEPCQ
                     GNCAAINPQDGFLQLIRKKTEEYGCVFILDEVKTGFRIANGGAQEYYRLKPDLVTYAK
                     ALGNGYPVAAFGGKKEIMSIIGHGVAQGGTYTNNKPGVAGAYATLSLLKSKPILKTIE
                     KRGQRLMDGLKEIFEENDIPAVFTGYPAMFSFSLNTEAVTCQRDWQKSDRDLYLKLTE
                     KAIQKGVMPDLDAREPWFLCYEHSDADIDETLNVYAEIVKEIKK"
     gene            853..2178
                     /locus_tag="PLG52_11520"
     CDS             853..2178
                     /locus_tag="PLG52_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB42121.1"
                     /translation="MPKLSTQEIVDLSKEYTFFSWSVQGQASPIPVERAEGIYFWDTD
                     GKRYIDFSSQLMNTNIGHQHPKVVKAIQDQAAKLCFVHPGNTTDVRALLGKKLAEVTP
                     GNLKKTFFSLGGAEANENAIKMARFYTGRHKILAQYRSYHGATHGSIALTGDYRRLAV
                     EPTMPGGVHFMNPHCYRCPFGQKKESCKRECISHLEEVIKYEGPDKIAAIIMEGVVGS
                     NGIIVPPDDYWPRVREICDKYGILLISDEVMSGWGRTGEWFAVDNWKVVPDMITTAKG
                     ITSGYAPLGAVIVSDKIAEFFEDKYLYAGLTYNGHALSCAAALATIEVYDEDHLMENA
                     KKMGNYLGECLEDIKAKHPSVGDVRYIGLFSTIELVANRDTKEIMSPAVMAEVGKTLR
                     ANGLFTFIMANNMGSMLFVVPPLCITKEQLDEGLAIVEKALEVADKQIK"
BASE COUNT          627 a          563 c          629 g          554 t
ORIGIN      
        1 agcctcttcg ggtattccca aaatgatgcg cgagaatatc atcacgctgc cattcaatga
       61 ttttgaaggc ttcgagcgtg tgatgcggtc atatggtgag caaattgctg cagttatttc
      121 tgagccgtgt cagggcaatt gcgcggcaat caatccgcaa gatggcttct tgcaacttat
      181 ccgcaaaaag acagaagaat atggctgtgt ctttatcttg gatgaagtga agacaggctt
      241 tcgtattgcc aatggtggag cgcaggaata ctaccgcctc aaaccggacc tcgtgaccta
      301 tgccaaagcc ctaggcaatg gctaccctgt cgctgcgttt ggcggtaaaa aagagatcat
      361 gtctatcatc gggcatggag tggcgcaagg cggcacctat accaataaca agcccggtgt
      421 ggcgggtgct tatgcaacac tgagcctgct caaatccaaa ccgattctca agaccatcga
      481 aaaacgcgga caacgcctca tggatgggct gaaggaaatc ttcgaagaga atgatatccc
      541 tgctgtgttt acgggctatc ccgccatgtt ctccttctcg ttaaataccg aggcggtgac
      601 ttgtcagcgt gattggcaga aaagtgaccg tgacctgtac ctcaagctga cagagaaggc
      661 gatacaaaaa ggtgtgatgc ccgacctcga tgcgcgtgag ccgtggttct tatgttatga
      721 acactccgat gccgacattg atgagacgtt gaatgtctat gccgagatcg tgaaggaaat
      781 aaagaaatag tcaaaggtca aaagtcgaag gttgttgact ttacaccttt gacttttgac
      841 atgaggaaac ttatgccaaa attatctact caagaaattg ttgatctcag caaggaatac
      901 actttcttct cctggtctgt gcagggacaa gccagcccga tcccggttga gagagccgaa
      961 gggatttact tttgggatac agacggaaaa cgttatattg atttttcatc ccaattgatg
     1021 aataccaata ttgggcatca gcacccaaag gtggtgaaag ccattcaaga tcaagcggcg
     1081 aaactttgct ttgtacaccc gggcaatacg acagatgtcc gtgccttgtt gggaaaaaaa
     1141 ctggcagaag tgacccccgg caatttgaag aaaaccttct tctccctcgg tggcgcggaa
     1201 gcgaacgaga atgccatcaa gatggcgcgt ttctacacgg ggcgtcataa gatcctggcg
     1261 caataccgtt cctatcacgg cgcgactcac ggctcgattg ccctgacggg tgattaccgc
     1321 cgccttgccg tcgaaccgac catgccgggc ggggtgcatt tcatgaaccc acattgctat
     1381 cgctgtccgt tcgggcagaa gaaagaatct tgcaaacgcg agtgtatttc gcacttggaa
     1441 gaagtcatca agtacgaagg tcccgacaag atcgcggcaa tcatcatgga aggggtggtt
     1501 ggttcaaacg gcatcatcgt cccgccggat gattactggc cccgtgtccg cgaaatctgt
     1561 gataagtacg gcatcctgct catctccgac gaagtcatga gtggatgggg acgcaccggc
     1621 gaatggttcg ctgtggataa ctggaaggtg gtcccagaca tgatcaccac cgccaaaggc
     1681 atcaccagcg gatacgctcc gctcggtgct gtcatcgtct cagataagat cgccgaattc
     1741 tttgaagata aatatctcta tgctggtttg acgtataacg gtcatgcctt atcttgtgcg
     1801 gcggcgcttg ccaccatcga agtttatgat gaggatcacc tcatggagaa cgccaaaaag
     1861 atgggcaatt atttgggtga atgccttgaa gatatcaaag ccaaacatcc atctgtgggc
     1921 gatgtgcgtt atatcgggct tttctcaacc attgaattgg tcgcaaatcg cgacacgaaa
     1981 gagattatgt ctccggcagt gatggcggaa gttggcaaga ccctgcgggc aaatggtctt
     2041 ttcactttca tcatggcgaa caacatgggc agtatgctct ttgtcgtgcc gccgttgtgc
     2101 atcaccaaag agcaactcga cgaaggtctt gccattgttg aaaaggcatt ggaagttgcg
     2161 gacaagcaaa ttaaataaat tcgtaggggc gcggttaccg cgcccctaca tggagaaccc
     2221 atggaaatcc ttaattacat caacggcgaa tgggtcaagc ccagcgtcaa agattatttc
     2281 gacgtgatca acccggctac tgggctggtg atcgcaaaga ctccgcttgg cacaaaagcg
     2341 gatgtggaag cagcggcgag agcggcgagt gag
//
LOCUS       DAOQNJ010000453         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_35642_length_2366_cov_9.808308, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000453 DAOQNJ010000000
VERSION     DAOQNJ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35642_length_2366_cov_9.808308"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLG52_11525"
     CDS             <1..941
                     /locus_tag="PLG52_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNB42122.1"
                     /translation="AGLILTPYFTTRPARAFRSLLGRMTAESLFAGLTGLVVGLLIAA
                     LLAFPLSMLPQPFSQILPFIGVLIFAYFGVSIFVMRQGDIMGLISSLSGRTEGGGSSS
                     WTNLNRTILLDTSVIIDGRVSDIAKTGFLPGTLLIPRFVLNELQYIADSSDGVRRQRG
                     RRGMEVLAELQKLPNVLVRISDINVDGVREVDDKLVVLGKQLKSPILTNDYNLNRVAE
                     LQGVTVLNINELANAVKSVVLPGEALRINIMQEGKEYGQGVGYMDDGTMVVVENGRDY
                     IGEYMAVNITKVLQTAAGRMIFGRVEEENGKKAKSK"
     gene            1100..>2366
                     /gene="cysS"
                     /locus_tag="PLG52_11530"
     CDS             1100..>2366
                     /gene="cysS"
                     /locus_tag="PLG52_11530"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNB42123.1"
                     /translation="MLKLYNTLTRKTEEFQTLEPNKVKMYVCGVTVYNDAHVGHAMSA
                     IVFDIIRRYLEYRGYAVKHVMNYTDVDDKIINRANQLGEDPLKLSARYIADYAADLKS
                     LNVLPATSNPQVSTTMPLIIQFIEGLIQKGHAYAAENGDVYFRVNSDDDYGRLSGRKL
                     EDMQAGARIEVGEQKEHPMDFALWKAAKPGEISWDSPWGKGRPGWHIECSAMNLAELG
                     EQIDIHGGGNDLIFPHHENEIAQSESYTGKEFSRYWIHNGMLQLGGEKMSKSLGNIIS
                     IKDFLSKRSSDVMRMLVLNGTYRAPLMFNDETLDAAERNVERLKSALKPASVSAAGLN
                     ADTLSASIQSASTNFTDAMDNDFNTAGAVAALFELSKSINTARDNGATADQLKPAQET
                     FKQLAGVLGLKLEEKKGSSEKEAEVEALLA"
BASE COUNT          609 a          653 c          583 g          521 t
ORIGIN      
        1 tggcaggcct gatcctcact ccatacttta ccacccgacc agcccgcgct ttccgttccc
       61 tgttgggcag aatgaccgcc gaaagtctgt ttgcggggct aaccggactc gtcgttggtt
      121 tgctgatcgc tgcccttctg gccttcccac tttcgatgtt gccgcaacct tttagccaga
      181 tattaccgtt tattggtgta ttgatctttg cctactttgg cgtttcgatc ttcgtgatgc
      241 gtcagggtga cattatggga ttgatcagtt cattgagcgg acgcacagaa ggcggcggtt
      301 cctcctcgtg gacaaacctt aaccgcacca ttttgcttga tacaagtgtc atcatagatg
      361 ggcgtgtctc agacattgcc aaaaccggtt tcctgccagg cacgttgctt attccgcgtt
      421 ttgttctcaa cgaactgcaa tatattgccg actcatccga cggtgtacgc cgtcaacgcg
      481 ggcggcgcgg catggaagtg ctggctgaac tgcaaaaatt gcccaatgtc cttgtccgca
      541 tctcagacat taacgtggat ggcgtgcgcg aagtggacga taaactggta gtgcttggca
      601 aacaactcaa gagtcccatc ctcaccaatg actacaatct caatcgtgtg gctgaactgc
      661 aaggcgtgac cgtgctcaac atcaacgaac tggcgaatgc tgtcaaatcc gtggtgttgc
      721 ctggtgaagc cctacggatc aacattatgc aggaaggcaa ggaatatggc cagggcgtag
      781 gttacatgga cgatggcacg atggtcgtgg tcgaaaatgg acgggactac atcggcgaat
      841 atatggctgt gaatatcacc aaggttctgc aaaccgccgc cggacgcatg atcttcggac
      901 gggttgaaga agaaaacgga aaaaaagcca agagcaagta ataagaaacg agtaacgagt
      961 agtcagtaaa cagggcgtcg agaggcgtcc tgttttttta gaagaacaga ccgccgacgg
     1021 cagtctgtgg tcgacggtct gccgtctagg gtaaaataca cccggctaca aaatcctttg
     1081 cgcccttatg gtaaataaaa tgcttaaact atataacact ctcacccgca aaactgaaga
     1141 attccagaca ctcgaaccta ataaagtcaa gatgtatgtc tgtggtgtca cggtttataa
     1201 cgatgcgcat gttgggcatg ccatgtctgc catcgtgttc gatatcattc gtcgctacct
     1261 cgagtatcgt ggatacgccg tcaagcacgt gatgaactac actgatgtgg acgacaagat
     1321 tattaatcgc gccaaccaac ttggtgaaga cccactcaaa ctttcggctc gttacatcgc
     1381 cgactatgcc gctgacctca aaagcctcaa tgtgttgcct gccacgtcca acccacaggt
     1441 cagcaccacc atgccgctca tcattcaatt catcgaaggg ctgatccaaa aaggtcacgc
     1501 ctacgccgca gaaaatggag atgtctactt ccgcgtgaac agtgacgatg attatggtcg
     1561 tctctcgggt cgcaaactcg aagacatgca agccggtgcg cgcatcgaag tgggcgaaca
     1621 aaaagaacac cccatggact tcgccctctg gaaagctgcc aagcccggtg aaatctcatg
     1681 ggacagtccc tggggcaagg gtcgccctgg ctggcacatc gaatgctctg ccatgaacct
     1741 cgccgaactc ggcgaacaga ttgacatcca cggcggcggc aacgacctga tcttcccgca
     1801 tcacgaaaac gaaatcgccc aaagcgaaag ctacaccggc aaagaattct cgcgttattg
     1861 gatccacaac ggcatgctgc aactcggcgg cgagaaaatg tccaagtcgc tgggcaacat
     1921 catcagcatc aaagacttcc tctccaaacg ttcttccgat gtgatgcgca tgttggtctt
     1981 gaacggaacc tatcgtgccc cgctgatgtt caacgacgaa acactagatg ctgccgaacg
     2041 caacgttgaa cgcctcaaat ccgcgctcaa gcctgcttct gtttccgcgg ctggactaaa
     2101 cgctgatacc ttgtccgctt cgatccaatc cgcatccact aacttcaccg atgccatgga
     2161 caacgacttc aacactgctg gagccgtcgc cgcgcttttc gaactttcca aatccatcaa
     2221 caccgcccgc gataacggcg cgacagccga ccaactcaaa ccggcacaag agaccttcaa
     2281 acaattggct ggcgtgcttg gcttgaaact ggaagagaaa aaaggctcca gcgaaaaaga
     2341 agccgaagtg gaagccctcc tcgccg
//
LOCUS       DAOQNJ010000454         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_35879_length_2355_cov_6.197391, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000454 DAOQNJ010000000
VERSION     DAOQNJ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35879_length_2355_cov_6.197391"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLG52_11535"
     CDS             complement(<1..410)
                     /locus_tag="PLG52_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42124.1"
                     /translation="MQKFIQPLIEKLKPFLADLQAVMDRLRTKIPGLPSNRVLFSTAA
                     VVVVAIAIFVALQILVAPDQPVTTYTLPYTQTFDEVNLKRWFSDSGVWTIRTGTLMQT
                     VGGEEAGQMHIPLKVPEDAPYHASVFITLKKDTK"
     gene            complement(548..>2355)
                     /locus_tag="PLG52_11540"
     CDS             complement(548..>2355)
                     /locus_tag="PLG52_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42125.1"
                     /translation="FRNVMYVVTFFTMWATMGYSGASWFPTFHRTMAFISTTANGMQS
                     GIGDLIVKLMGTIVQVLRFPHRFELVTFMVATILISLTLVWLHDTFMKKGFGEIVWVL
                     TGRRIGEVKAGKQKQVRAGWEAGRFIPVMMALLFMVPIFSNESYRVVFSSGDFHHFLT
                     PYPVGPLKEVKEALLQLPPGKVVVLPPTETAKVIVDINGVEHKFIDKFHIYYLDLPSY
                     YYGLTGDSDNKHEFFLMLRALYYQQPWWINIARDLDLKYIVVNKELVANTVGGQEYLR
                     EVERILIPELDSRDAYLNKIMENESYALYEFTDLPTAERVPLYLDVDWNSFIRILSSN
                     LELTRYYDLRHTMMVGDLEEFDSLTLVTDDVHESALDLFEKKNKAQFFRPSSVILPFD
                     PEQISSSYYLSPMFRLFQFFSDSKYNRLDMITPGLWGTLEGGFIGVPREAPFRIDVTL
                     PEEGEYRLLLRGAVSAVDMEMESRLFAEPQRISLASDPSNLAFFDKRLVFSSGRTPLD
                     VSQYTYKELGLLIPTDVVVVNYQYQFFDLGVVTAAKGKYPIYFNKLNDAPMLVEGILV
                     VPEEVYQSLTLPDNVTVVQPNELCCGSIIIQGEEP"
BASE COUNT          642 a          668 c          520 g          525 t
ORIGIN      
        1 tgtttggtat cttttttcag cgtgatgaat acgctagcat gataaggcgc atcttccggc
       61 actttgagcg gaatgtgcat ctgccccgcc tcttcaccgc cgacggtttg catcaacgtc
      121 cccgtgcgga tcgtccacac cccagagtct gagaaccaac gcttgaggtt gacctcatca
      181 aatgtttggg tgtagggcag ggtgtaggtg gtcaccggct gatcgggcgc caccaaaatt
      241 tgcagcgcca caaagatcgc aatcgcaacc acgaccactg cagccgtcga gaataacacg
      301 cggttcgaag gcagaccggg gatcttcgtc cgcaatctat ccatcaccgc ctgaaggtcg
      361 gcaaggaatg gtttcagttt ttcaatgagg ggttggatga acttctgcat atattctttc
      421 tctatgcgta ttctatgact tatgggctac gaggagcatt tcattctcat tctcaattta
      481 ctgacgaaaa tgcaaattca gaatcaaaca acaattccca accataccca accttctcca
      541 acaaacacta cggctcttcc ccttgaataa tgatcgaacc acaacacaac tcattcggtt
      601 gaaccactgt cacattatca ggcagtgtta aagactgata cacctcctca gggacgacca
      661 aaatcccttc caccagcatg ggcgcatcgt tgagcttgtt gaaatagatc ggatatttac
      721 cctttgcagc cgtgaccacg cccagatcga aaaattggta ctgataattc accaccacca
      781 catcagtggg gatgagcaat cctaattctt tatacgtata ttggctcacg tccaacggcg
      841 tccgccccga ggagaagaca agtcgtttat cgaagaatgc caaattcgac gggtctgaag
      901 cgagcgaaat ccgctgaggc tcagcaaaca gcctggactc catttccata tccacagccg
      961 ataccgcccc gcgcaacaac aagcgatatt caccttcctc gggcagggtt acatcgatgc
     1021 ggaagggcgc ttcacgcggt acaccgataa acccaccctc cagcgtgccc cacaaaccag
     1081 gcgtgatcat atccaggcgg ttgtacttgc tgtctgagaa aaattgaaac aagcggaaca
     1141 tcggcgagag ataataggaa ctggatatct gttccggatc gaacggcagg atcacgctgc
     1201 ttgggcggaa gaattgggct ttattcttct tctcaaacag atccagcgcc gactcatgca
     1261 catcatccgt caccagtgtc aacgagtcaa attcctcgag atcgccgacc atcatcgtat
     1321 gacgcagatc gtaataacga gtcaactcca ggttagacga caaaatacgg atgaacgaat
     1381 tccaatccac atccagatac aacggcacac gctcggcagt aggcagatca gtaaattcgt
     1441 acagagcata gctttcgttt tccattattt tatttaaata agcatcgcgc gaatccaact
     1501 cgggtatcaa aatgcgctcc acctcgcgca aatattcctg cccacccacg gtgtttgcca
     1561 ccagttcctt gttcaccaca atgtatttca aatccaggtc gcgcgcaata ttgatccacc
     1621 acggttgttg gtagtacaac gcccgcaaca tcaaaaagaa ctcgtgcttg ttatccgagt
     1681 cgccggtcag cccgtagtaa taactcggca ggtcaagata ataaatatgg aacttgtcaa
     1741 tgaatttatg ctccaccccg ttaatgtcca cgatcacctt ggcggtctca gtgggcggca
     1801 gcaccaccac cttgccgggc ggcaattgca gcaacgcctc cttcacttcc tttaatggac
     1861 ccaccggata gggtgtgagg aagtgatgaa aatctcccga cgaaaataca acccgatagg
     1921 attcattcga aaaaatgggg accatgaaca ataacgccat catcacggga atgaaccgcc
     1981 ccgcctccca cccagcccgc acctgtttct gcttcccagc cttcacctcc ccgattcgtc
     2041 tgcccgtcag aacccacacg atctcgccaa agcctttttt catgaatgta tcatgcagcc
     2101 agacaagcgt aagggaaata aggatggtcg ccaccatgaa agtgaccagt tcaaagcggt
     2161 gcggaaaacg aagcacctgc acgatcgtgc ccatcaactt cacgatcagg tcaccgatac
     2221 ccgactgcat tccattcgcc gtggtgctga taaatgccat agtgcggtga aaggtcggaa
     2281 accagctcgc acccgaataa cccatggtcg cccacatggt gaagaatgtg accacataca
     2341 tcacattgcg gaagg
//
LOCUS       DAOQNJ010000455         2353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_35938_length_2353_cov_7.444299, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000455 DAOQNJ010000000
VERSION     DAOQNJ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35938_length_2353_cov_7.444299"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLG52_11545"
     CDS             <1..427
                     /locus_tag="PLG52_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNB42126.1"
                     /translation="IIVSGQLLAVFLNNSGGAWDNAKKLIEDEPKDPAKNLGKGSERH
                     KAGVVGDTVGDPFKDTAGPALNPMIKVVNLVAVIVAPIVVQYQGLNLGTGVIAALLLG
                     VLAWAVMRSKAPAQSMTAAVSAPAPAKKAAAPKKKTTKK"
     gene            532..1494
                     /locus_tag="PLG52_11550"
     CDS             532..1494
                     /locus_tag="PLG52_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNB42127.1"
                     /translation="MADVKAFGERVMTSLEKVIVGKRQSLELVVIGLLCQGHILIEDV
                     PGVGKTMLARSLARSLDCIFNRIQFTPDMLPSDVTGVSIYNQQKNEFEFRPGPIIGQV
                     ILADEINRATPKTQAALLEAMEERQVTVDGVTHAMPKPYIVLATQNPIEYEGTFPLPE
                     AQLDRFLLRMRLGYPSAADEIRIMDDQRLQHPLEGLTPVASGDEVLQMSEAVRQVFIS
                     EVVKKYIVDLSTRTRTSQDVYLGASPRGSLFLSRAAQARAALRGRDHVLPDDVKALAV
                     AVLAHRIIVSAAARLRDVSSDRIVQEIVQSTPVPGGSFEATAKG"
     gene            1502..>2353
                     /locus_tag="PLG52_11555"
     CDS             1502..>2353
                     /locus_tag="PLG52_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNB42128.1"
                     /translation="MVKAGRAFIAIMFFVGLIGVLVNGAPIFSRFLYSSVLLALFAWG
                     WTRLIIRSLSFERVTRELRANVGDVFEETFKLSNRSRLPIPWVEIVNKSTLPFAAGSR
                     LFTLVPGKQFRSYMARTWLTRRGRFELGPTRITVGDPFGLFATSKEHKSKDALIVFPM
                     IHEIYAFQTPQGVLTGGQVIRKKSPDITPHAAGVREYVSGDAMKRIHWSTSARRNRLM
                     VKEFEQDPQAEVWLYLDAHQTVHYQKRHEQQEMALHDMLLNKRPKIKLPPSTLEYGVS
                     IAASLAHY"
BASE COUNT          556 a          534 c          657 g          606 t
ORIGIN      
        1 catcattgtt tccggtcagt tgttggcggt attcctcaac aactcgggtg gtgcttggga
       61 taatgccaag aagttgatcg aagacgaacc caaagatcct gccaagaatc ttggtaaggg
      121 ctccgaacgc cacaaggctg gcgtggtagg tgacaccgtt ggtgatccgt tcaaggatac
      181 tgccggtccg gctcttaacc cgatgatcaa agtggtgaac cttgtggctg tgatcgttgc
      241 cccgatcgtg gtgcaatatc agggcttgaa cttgggcacc ggcgtgattg ctgcattgtt
      301 gctcggtgtg ctggcttggg cggtaatgcg ctccaaggct cctgcgcagt ccatgaccgc
      361 tgcggtttcc gctccggctc ctgcgaagaa agctgctgct cccaagaaga agaccaccaa
      421 gaagtagtag aaaatcataa aaaaatcccg tcctgtagta ttagggcggg atttttttat
      481 gaaggcgcgt gatagaatcg aattatcttt ccttgaaagg aatcgtatca aatggcggat
      541 gtgaaagcat ttggcgagcg cgtgatgacc agtttggaaa aggtgatcgt cgggaaacga
      601 caatctcttg agttggtagt gatcggtctt ttatgtcagg ggcacattct gatagaagac
      661 gttcctggtg tagggaagac aatgttagcg cgcagtttgg cgcgttcttt agattgcatt
      721 ttcaaccgca tccagtttac tcctgatatg ctccccagtg atgtgacggg cgtttctatt
      781 tataaccagc agaaaaatga atttgaattc cgtccgggtc cgattattgg acaggtgatt
      841 ttagcggatg aaattaaccg tgccaccccg aagacgcaag ccgctttgct tgaagcgatg
      901 gaagaacgtc aggtgactgt ggatggcgtg acgcatgcca tgccaaagcc atatattgtg
      961 ctggctactc agaaccccat tgaatatgaa gggacatttc cgctccctga agcgcaatta
     1021 gatcgtttcc tacttcgtat gaggcttggc tacccaagcg ctgcggatga aattcgtatt
     1081 atggacgatc agagattgca acatccgctc gagggactaa cgcctgttgc ttcgggggat
     1141 gaagttttgc aaatgagtga agcggttcgg caggtattta tttctgaggt tgtgaaaaaa
     1201 tacatcgtgg atttaagtac tcgtactcgt actagtcagg atgtatacct cggtgccagc
     1261 ccacgtggaa gcttgttcct ctcgcgagcg gctcaagccc gagccgccct acgcggacgt
     1321 gaccatgttc tgccagacga cgttaaggct ttggcggtgg cagttctggc gcatcgtatt
     1381 attgtcagtg cggcagccag gttgcgcgat gtgagttcag accgtatcgt tcaggagatt
     1441 gtgcagtcca cgccggtgcc aggtgggtcg ttcgaagcca cggcaaaggg ataacccaaa
     1501 catggtgaaa gccgggcgag cctttatcgc catcatgttc tttgtagggc tgatcggtgt
     1561 tctggttaat ggggcaccga tcttttcacg ttttttgtat agcagtgttt tgcttgccct
     1621 ctttgcctgg ggttggacgc gcctcattat ccgcagtttg agctttgagc gtgtgacgcg
     1681 tgaactacgt gccaatgtgg gggatgtatt cgaagaaacc ttcaagctaa gtaatcgcag
     1741 tcggttgccc attccatggg tggagattgt gaacaaatcc acgctgccat ttgcagcggg
     1801 gtcacgtttg tttaccttgg tgccgggtaa acaattccgc agctacatgg cgcggacttg
     1861 gctcactcgc cgtggacgat ttgaattagg tccaacgcgc atcacagtag gagacccatt
     1921 tggattgttc gcgacaagca aagaacacaa atccaaagat gccttgatcg ttttcccgat
     1981 gatccatgag atttacgcct tccagactcc acaaggcgtg ttaactggcg gacaggtgat
     2041 tcgaaagaaa tcgccagata ttactccgca tgcggcgggc gtacgtgaat atgtgagtgg
     2101 cgatgccatg aagcgcattc attggtctac aagcgcgcga cgtaaccgtt tgatggtgaa
     2161 agaatttgag caagaccccc aagcagaagt gtggttatat cttgatgcgc accaaacggt
     2221 tcattatcag aaacggcatg aacagcaaga aatggcgttg catgatatgt tgttgaacaa
     2281 gcgccccaag atcaaattgc cgccttctac tttggaatat ggggtgagca ttgcggcatc
     2341 actagcgcat tac
//
LOCUS       DAOQNJ010000456         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_36054_length_2349_cov_9.194420, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000456 DAOQNJ010000000
VERSION     DAOQNJ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36054_length_2349_cov_9.194420"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2246)
                     /gene="priA"
                     /locus_tag="PLG52_11560"
     CDS             complement(<1..2246)
                     /gene="priA"
                     /locus_tag="PLG52_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNB42129.1"
                     /translation="MFARLAVNISAISGVFDYSIPAELVPQVRAGCLVTAPFGNQTVQ
                     GIIVELTDSSSVPNPKQILDLLDPAPLLTPPQLALAIHLADSTLNPLSAIIGMMIPTG
                     LSQQADTLYAVRTPEYSDPSAKVVTRILNLLHERGPLRGRQIDSHFAKIDWRKSANML
                     VKKGVLTSKNVLPPPRVRPKNIRVAQLAVTPEAAEEMMASLGSTKATQTRRESALRFL
                     IQQPEPIHLSWVYAQTGCNISDLQELEERGLIRLFENEIFRDPLERIEKQETKSELSL
                     TSEQNSALQAILSSFRSQPSGFLLYGVTGSGKTEIYLRAAQEVIKSGRQAIILVPEIA
                     LTPQAVRRFVSRFPGQVGLVHSKLSEGERYDTWRRARDGKLNVIIGARSALFAPLPNI
                     GLIVVDECHDSSYYQSEPPYYNAVTAAQEYAKLCGAVCVLGSATPTIEQRFLAEQNQI
                     TRLDLPNRVTESTLPPVQVVDMREELKNGNRGIFSKPLAESLAEVLERKEQAIVYLNR
                     RGTSTYIFCRDCGTTLKCPNCETPLTLHNEIHYSKGNTLLCHRCGYTRQMPKTCPTCA
                     STQIREYGLGSERVEAEIKSMFPQARTLRWDWDTTRQKDAHEIILTHFANHQADILVG
                     TQMLAKGLDLPLVTLVGIVLADVGLNLPDPFASERVFQTLTQVAGRAGRSSRGGKVIM
                     QTFTPDHYALQFAAGHNVDGFYERELEYRRSLGYPPFTKLVRLEYRNADNLKAEEEAK
                     KLASKLKV"
BASE COUNT          561 a          553 c          635 g          600 t
ORIGIN      
        1 ttgaccttga gtttgcttgc cagcttcttg gcttcttctt ccgctttgag attatccgca
       61 ttacgatatt ccaagcgcac taacttggta aatgggggat atcctaacga acggcgatac
      121 tccaactcgc gttcataaaa cccatctaca ttatgcccgg ctgcaaactg aagagcataa
      181 tggtctggtg taaaggtctg cataatgacc ttgccgccgc gtgaagaacg ccccgcccgc
      241 cctgccacct gcgtcaacgt ttgaaagacg cgttcacttg cgaaggggtc gggcaaattc
      301 aacccaacat ctgccaatac gattccaacc aaggtcacga ggggcaggtc taaccctttg
      361 gcgagcattt gcgtgcctac taaaatatcg gcttggtgat tggcaaagtg ggtgagtatg
      421 atctcgtgag catctttctg ccgagttgta tcccaatccc agcgcaaggt ccgcgcttgc
      481 gggaacatgg atttgatctc tgcttctacc cgttcacttc ccagcccata ctcgcggatt
      541 tgcgtgctcg cacaagtggg gcaagttttg ggcatttgac gggtgtatcc gcaacggtga
      601 cagagcaaag tattgccttt ggaataatga atctcattat gcaatgtcag cggtgtttcg
      661 cagttgggac atttcaatgt cgtaccacaa tcgcggcaga aaatatatgt cgatgttccg
      721 cgccgattga gatagacgat ggcttgttct ttgcgctcca agacttcggc tagtgactcg
      781 gctaatggtt tcgagaaaat gccgcggtta ccgttcttca actcctcacg catgtccact
      841 acttgcaccg gcggcaatgt agactcggta acacggttgg gcaagtcgag ccgtgtgatc
      901 tggttttgct ccgcaaggaa acgctgctca atcgtgggcg tggcagagcc cagcacacaa
      961 accgcgccac aaagttttgc atattcctgt gcggcggtca cggcgttgta ataaggcggt
     1021 tcgctttgat agtatgaact gtcatggcat tcatccacga cgatgagacc aatatttggt
     1081 aaaggcgcaa acaacgccga acgagcccca ataatgacgt tcaacttccc atcgcgggct
     1141 cgacgccatg tatcatatcg ttccccttca gaaagcttcg agtgaaccaa accgacttga
     1201 cccggaaaac gtgagacaaa gcgccgcaca gcttgcgggg tgagcgcaat ttcaggcaca
     1261 agaatgatgg cttgtcttcc gcttttgatc acttcttgtg cggcgcgtaa gtaaatttcc
     1321 gttttgccag aaccggtcac gccgtataaa agaaacccag aaggttgaga gcggaaggat
     1381 gaaagaatgg cttgcaaagc agaattttgt tctgatgtaa gcgataactc actctttgtt
     1441 tcttgtttct caattctctc caacgggtcg cgaaagattt cattttcaaa caggcgaatc
     1501 agaccgcgtt cttcaagctc ttgcaagtct gaaatattgc agcctgtttg cgcatacacc
     1561 catgaaagat gaatcggttc gggctgttgg atgaggaagc gtagagcgga ttcgcgtcga
     1621 gtctgagtcg ctttcgtgga accaagcgat gccatcattt cttctgccgc ctctggcgtg
     1681 actgcgagtt gggcaacgcg aatattcttg ggtctcactc tgggcggagg caagacattc
     1741 tttgaggtta gcacaccttt tttgacgagc atgttggcag acttgcgcca gtctattttt
     1801 gcgaaatgag aatcgatttg gcgtccgcgt aatggtccgc gttcgtggag taggttaagg
     1861 atgcgggtga caacttttgc agatgggtct gaatattcag gagttcgtac tgcgtaaaga
     1921 gtatctgctt gctggctgag cccggtgggg atcatcatgc caatgatggc agagagcgga
     1981 ttcagtgtag agtctgctag gtgaatggca agagcaagct gaggcggggt gagaagcggg
     2041 gcagggtcta gcaggtcaag gatctgttta ggattcggta ccgaagaaga atcggtcaac
     2101 tcgacgataa tgccctgcac agtctgattt ccaaatggtg ctgtaacaag acaccccgcc
     2161 cgaacctgtg gaacaagctc ggcagggatg ctgtaatcga acacgccaga gatggctgag
     2221 atattgacgg caagacgggc aaacatgaga cgattataat tgtttcaatg gaggctgacc
     2281 tatgagcatt gtaattcttg ttgctggcgc tttgatgatt ttgatcgcat tggggttgtt
     2341 ggcgatggt
//
LOCUS       DAOQNJ010000457         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_36322_length_2339_cov_10.582750, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000457 DAOQNJ010000000
VERSION     DAOQNJ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36322_length_2339_cov_10.582750"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..877)
                     /locus_tag="PLG52_11565"
     CDS             complement(14..877)
                     /locus_tag="PLG52_11565"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNB42130.1"
                     /translation="MKVLVTGGTGFTGSRLIPLLLKNGIQVRALVRPTSNRSPLSALT
                     VEWVTGDLADAESFTAALRGVDALVNIASLGFGHAESIVRAAKEAGVKRGLFISTTAI
                     FTQLNAGSKSIRLAAEEAIQASGLEYTILRPTMIYGSPRDRNMWRLIRLLKIIPIIPI
                     FGDGESLQQPIFVDDVAQAVLLALQNDATIHKSYNIAGKDPLTYNQVIDMVASALGKR
                     VWKLHLPYMPIVRALQFTERMRLRLPIKAEQVLRLNEDKSFSYEDAKRDFGFTSRSFE
                     EGIRAEVGTKK"
     gene            complement(874..2094)
                     /locus_tag="PLG52_11570"
     CDS             complement(874..2094)
                     /locus_tag="PLG52_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB42131.1"
                     /translation="MKILLINQVFVSPNEPGHTRHFEMGQYLKERGHELIIVASDLNY
                     QTGQRTVERRGLFAEQVFDGVRVLRTYIYPAIHRSYFWRIIAFFSFMFSSILTALRVK
                     DVDLVLGTTPPIFQAVSALVVASLRGKPFLLEVRDLWPEFGISMGVLKNPIVIGLSRW
                     LENFLYKRANHILVNSPAYREYMLAKGVPANKVTYIPYGTDVDMFNPSIDGSSIRREL
                     NLEDQFMVLYAGALGQANDIDTLLRAAERLKKEERIKFVLFGDGKEKTRLVTEAQNKN
                     LTNVIFAGTRPKKDMPLIVASADACLAILQDIPAFRTTYPNKVFDYMAAGRPSIIVID
                     GITRELIESSHGGVYVTPADDAMLAQKILELAHNPEQAKTMGANARDYLVKNLDRRDK
                     LSETLSLLEKLVKA"
BASE COUNT          582 a          554 c          618 g          585 t
ORIGIN      
        1 ttcggagacc tttttatttc ttcgtgccaa cctcggcgcg tattccctct tcaaagctgc
       61 gcgaagtgaa accaaaatct cgcttcgcat cttcatagga gaatgactta tcctcgttca
      121 gcctcaacac ttgttcggct ttgatcggca aacgcaatcg catccgctct gtgaattgca
      181 atgctcgcac aattggcata tagggcaggt gcaacttcca aactcgcttg ccaagtgcag
      241 aggcaaccat atcgatgacc tggttatagg tcaacgggtc tttgcctgcg atgttgtaac
      301 tcttatgaat ggtcgcatca ttctgcaaag caagtagcac agcttgagct acatcgtcta
      361 cgaagatggg ttgttgcaaa gactcgccgt caccgaagat gggtatgatg ggtataattt
      421 tgagcaggcg gatcagccgc cacatattgc ggtcacgcgg actgccatag atcatggttg
      481 ggcgcaggat ggtgtactct agcccgctgg cttgtatggc ttcttcggcg gcaaggcgga
      541 tggatttgct acccgcattc aactgcgtga agatggcggt ggtgctgatg aaaagtccgc
      601 gcttcacgcc cgcctctttt gccgccctga caattgattc tgcgtgccca aacccgagcg
      661 atgcgatgtt gacaagagcg tccacgccgc gcagggcggc ggtgaatgat tcggcgtcgg
      721 ctaaatcacc tgttacccac tccactgtca gcgcggaaag aggcgaccgg ttgcttgtgg
      781 ggcgcaccaa agcgcggact tggattccgt tctttagcag gagcgggatg aggcgtgagc
      841 cggtaaatcc agtgccgcct gtgactaaga ccttcatgcc ttcaccaatt tttccagcaa
      901 gctcaaggtt tcacttaact tgtcgcgtcg gtctaagttc ttgaccagat aatcgcgtgc
      961 attcgcgccc atggttttgg cttgttctgg attgtgagcc agctcaagaa ttttctgcgc
     1021 cagcatggca tcatcagctg gcgttacgta cacaccgcca tgcgaagact caatcaattc
     1081 acgagtaatg ccatcaatca cgatgatgct cgggcgaccc gccgccatat agtcaaagac
     1141 tttgtttggg taggtggtgc gaaaggcagg gatgtcctgc aaaatagcga ggcaggcatc
     1201 ggctgaggca acaatgagcg gcatatcttt cttcgggcgt gtgcctgcaa agataacgtt
     1261 ggtgagattt ttattttgtg cttcggttac aaggcgtgtt ttttctttac catcgccaaa
     1321 taatacaaac ttaattcttt cttctttctt taaacgttcc gccgcacgca gcaaggtatc
     1381 aatgtcgttg gcttgaccta atgcccccgc atacaaaacc atgaattgat cttcgaggtt
     1441 gagttctcta cgaatggacg aaccatcgat ggacgggttg aacatgtcca cgtcggtgcc
     1501 atagggaatg taggtgacct tgtttgcggg cacacctttg gcaagcatgt actcacgata
     1561 ggcaggtgaa ttgaccaaga tgtgattagc gcgtttgtat aagaagtttt caagccaacg
     1621 tgaaagccca atgacgatgg gatttttcaa cacacccatg ctgataccaa actcgggcca
     1681 taaatcacgg acttcaagca agaacggttt gccccgcagc gaagcgacaa ccaaagctga
     1741 aacggcttga aagatcggag gtgtggtgcc caagacaagg tccacatctt tgacgcgtag
     1801 agcggtgagg atcgaggaaa acatgaagct aaagaaagcg atgatgcgcc aaaaataact
     1861 acgatgaatg gcaggataaa tataggttct gagcacacgc acgccatcaa atacttgttc
     1921 agcaaacaag ccacggcgtt ctacggtgcg ttgaccggtc tggtagttaa ggtcactggc
     1981 aacgatgatg agctcgtgtc cgcgctcttt gaggtactgc cccatttcga agtggcgggt
     2041 gtgcccgggt tcattgggcg agacaaagac ttggttgatg agtaatatct tcatgcgagg
     2101 agggattata aatgcagagt ggtcaatgaa gaatgatgaa tggcttgggg aaatttacac
     2161 tcgcagattt gatttgccaa ctttctatat tgttaatctg gctacgcgca gcgagataac
     2221 tctgagagtc tttgataggt tgcctgcgta gaattacagt caacgaaagg aattgcacac
     2281 atggaaatcc ctcgacactg gcgtctcaaa aaacaacgat atggtctcgt tggtgaggt
//
LOCUS       DAOQNJ010000458         2333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_36488_length_2333_cov_6.364794, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000458 DAOQNJ010000000
VERSION     DAOQNJ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36488_length_2333_cov_6.364794"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /gene="gltX"
                     /locus_tag="PLG52_11575"
     CDS             <1..1304
                     /gene="gltX"
                     /locus_tag="PLG52_11575"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNB42132.1"
                     /translation="MDGLRWLGLNYDEGPDIGGAYGPYRQTERREIYQAHAKTLVEKG
                     HAYPCFCTPERLDKVRKEQMDRKENPRYDGTCRNLAPDEAAKRIANGEAYTIRFKVPQ
                     DGVTVAHDHLRGDITIENKQLNDQVILKTDGLPTYHLAAMVDDHEMKITHVMRGSEWL
                     GTFPLHVNIVRAFGWDEPIWIHLSVFLKPSGKGKMSKREAADAMKDGYSIFVKDMQDL
                     GFTPEGVLNWCGLMGWGVAEDDVMTTAQMVDRFTIDSLTPSPAAINFQRLDHFNATHI
                     RLLTTEDLAARLKPYFTREGLAVKDDVLLKMIPLIRERLTTLDDCISFGAFFFKDEVT
                     LTPEDLIAKGLDAKQSAEIARKAYELLSAQPDISHERSEPPFREYVETSGFNAGQVFG
                     VLRVAVTGQKVSPPLFESMEIIGRDICLKRIQNAISILEKM"
     gene            complement(1334..1975)
                     /locus_tag="PLG52_11580"
     CDS             complement(1334..1975)
                     /locus_tag="PLG52_11580"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB42133.1"
                     /translation="MHIQPKDPALQEILLNYSQESDLVKRKQIEDEIWQRYGSANTAF
                     VLDMSGFSLLTRKYGIVHYLSMIRRMQLTVEPIIMGHGGRIIKFEADNCFAVFPEPAA
                     AARTAITIQHALAASNLLTSEDMDVHVAIGIDYGKILILNDDDMYGDAVNRACKMGED
                     IGKADEILISKEAMDMIPEDAQIEGKLIEVSIGGLTMPAYQIVYRTNTEEEQQ"
     gene            complement(2035..>2333)
                     /locus_tag="PLG52_11585"
     CDS             complement(2035..>2333)
                     /locus_tag="PLG52_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNB42134.1"
                     /translation="GDAGYVVRGRGVSESLESASHGAGRKMSRRVAINSISKRERDDY
                     LKERGVTLLGGGIDESPQAYKQIEEVITAQHDLVDVIGKFTPRIVRMADEPGDI"
BASE COUNT          567 a          589 c          581 g          596 t
ORIGIN      
        1 tcatggatgg tctgcgttgg cttggactga actacgatga aggtccagat attggcgggg
       61 catacggacc gtatcgtcag accgagaggc gagaaattta tcaagcccat gccaaaacgc
      121 ttgtagaaaa gggacatgca tacccttgtt tctgcacgcc tgagcgcttg gacaaggtcc
      181 gcaaggagca gatggatcgc aaagagaatc cacgctatga tggcacctgt cgcaacctag
      241 ctcctgatga agctgcaaag cgcattgcca atggtgaggc atataccatt cgtttcaaag
      301 ttccgcagga tggtgtgacc gttgcgcatg accacttgcg cggtgacatt accatcgaga
      361 acaaacaact caacgatcaa gtcattctca aaacagatgg cttgccgacc tatcatctcg
      421 ctgccatggt ggacgaccat gaaatgaaga tcacgcatgt gatgcgcggc tcagagtggc
      481 tgggcacctt cccgttgcat gtcaacattg tccgcgcctt tgggtgggat gaaccgattt
      541 ggatccatct ttccgttttt ctcaagccaa gcggcaaggg caaaatgtcc aaacgtgaag
      601 cggcagatgc catgaaagat ggctattcga ttttcgtgaa agacatgcaa gacctgggtt
      661 tcacccccga aggtgttttg aactggtgtg ggctgatggg ttggggtgtg gctgaagacg
      721 atgtgatgac cacggctcaa atggtggacc gcttcaccat cgactcattg acgccttctc
      781 cggctgcgat caacttccag cgcctcgacc acttcaacgc gactcacatc cgcctcctga
      841 cgaccgaaga cttggcagct cgtctcaagc cttacttcac ccgtgaggga ctcgctgtga
      901 aggacgacgt tctgttgaag atgattcctc tcatccgcga gcgccttaca actctggatg
      961 attgcatttc ctttggcgca tttttcttca aagacgaagt cacactgact cccgaagacc
     1021 tcatcgccaa agggctggat gcaaaacagt ctgcagagat cgctcgaaag gcatatgaac
     1081 tcctatctgc gcaaccagat atcagtcacg aaagaagcga accgcccttc cgcgaatatg
     1141 tcgaaactag cggattcaat gccgggcaag tctttggtgt gttacgcgtc gcagtgactg
     1201 gtcagaaggt cagtccgccg ttgtttgaga gtatggagat catcgggcgc gatatctgcc
     1261 tgaaacgcat tcaaaacgcg atttcaatat tggaaaagat gtaaaaaaag acggagcatt
     1321 tgctccgtct tttttattgc tgttcttctt ctgtattggt tcgataaaca atttgatacg
     1381 ccggcatggt cagcccgccg atgctgacct cgatcaattt accctcaatt tgagcatctt
     1441 ctggaatcat atccatggct tcttttgaaa tgaggatttc atcagctttg ccaatgtctt
     1501 ctcccatttt gcaggcacgg ttgacagcat cgccatacat atcatcgtcg ttgagaataa
     1561 ggatttttcc ataatcaatg ccgatggcaa catgcacatc catatcttca ctggtaagca
     1621 ggttagatgc cgctaacgca tgttgaatgg ttatggcagt tcgtgctgcc gctgcaggtt
     1681 ctggaaaaac tgcgaaacag ttatccgctt caaattttat aatgcgccca ccatgcccca
     1741 taatgatggg ttcaactgtt agttgcatac ggcgaatcat ggataaataa tgaacgatgc
     1801 catatttgcg agtgagcagt gagaaaccag acatatctaa aacaaaagca gtgttcgcgc
     1861 tgccgtatcg ctgccagatc tcatcttcga tctgcttacg tttcacaagg tccgattctt
     1921 gtgaatagtt tagtaagatt tcttgcaagg cagggtcttt gggttggata tgcataagtt
     1981 cattatatcg aaaagggtca cgagagtggc ttacattccc atgacccttt ttacctaaat
     2041 atctcccggc tcgtctgcca tgcggacgat gcgcggggtg aacttgccga tcacatccac
     2101 cagatcgtgc tgggcggtga tgacctcttc gatctgtttg tatgcctgtg gggattcgtc
     2161 gataccgccg ccgagcaggg tgacgccacg ttctttgagg taatcatccc tttcgcgctt
     2221 gctaatcgag ttgatggcaa ctctgcggct catcttgcga ccggctccgt gcgaagcaga
     2281 ctcaagcgac tcgctcacgc ctcttccgcg gacgacgtag cctgcgtctc cca
//
LOCUS       DAOQNJ010000459         2330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_36567_length_2330_cov_9.159560, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000459 DAOQNJ010000000
VERSION     DAOQNJ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36567_length_2330_cov_9.159560"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLG52_11590"
     CDS             <1..888
                     /locus_tag="PLG52_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB42135.1"
                     /translation="PRRVAWEPNLMTGVVHFLDPYRYRSTFHRTNPNISEADFAKDYV
                     NHLEEIILYEGPESIAGILMESVTGTNGIIIPPDGYMQGVRTLCDKYGIVMICDEVMS
                     GFGRTGKWFAIENWNVVPDMITMAKGLTSAYAPLGAVAMKPEIAAAFDEHAFESGLTY
                     TSHPISLAAAVANIQAMREDKVVEHAASMGGVLRRMLSDLGEAHPSVGEVRNIGLFGI
                     IELVKNRETKEPMAPWNGSSPEMVALKKYCSDHGLFLYTHWHTVLIIPPLIISEKELQ
                     EGFDVLDEALKITDRTSDG"
     gene            941..1252
                     /locus_tag="PLG52_11595"
     CDS             941..1252
                     /locus_tag="PLG52_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42136.1"
                     /translation="MESQPFNSSPAPAPASGGNERVMAIASLVIGVINLCAWFLPICG
                     APLGIIGIVLGIFGMKDPSQKTLAIIGIALSAIGLLAACINGAVGAYMGVTGQGFNFS
                     P"
     gene            complement(1303..>2330)
                     /locus_tag="PLG52_11600"
     CDS             complement(1303..>2330)
                     /locus_tag="PLG52_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNB42137.1"
                     /translation="VPSQNNNQLPNPSQPIGSVVFDDYNELVDNATVTISGIPLPPAG
                     QHFEVWFLGQGGEIKLNAGPVIFTGQNGQLIFSDSNGANLLSQFDEMMVTVEPDNDPN
                     PDQPSQEVAASSVFPPQAFVHIRHILVSIGMTPNGTALIQGVWDTADTIDTSVLELRD
                     AFNEGDEATLRIKNEEIINQLVGEENLQLYKDWNGDGEINDPNDGFGLLANGTEQGYI
                     PLTISHTEFAIDAPDATTGIIQSGQNLIVCLQNMEGWSEQLLEVALKLQSMPYGTGME
                     STIAEIETYSSLLLYGEDTNGNNRIEPLPGEAGADTAYFAAYSIAQMPIFPGGRRMPQ
                     PANTTQP"
BASE COUNT          551 a          474 c          612 g          693 t
ORIGIN      
        1 ccgcgccggg tggcttggga gccgaatttg atgacaggcg tggttcattt ccttgaccca
       61 tatcgatatc gatccacctt tcaccgtacc aacccgaata tcagtgaagc agattttgct
      121 aaggattatg tcaaccattt agaagagatc attctgtatg aaggtcccga gtctattgca
      181 ggcatcttaa tggagtctgt cacaggcacg aatggcatca tcattccgcc agatggatac
      241 atgcagggag ttcgtacgtt atgcgataaa tacggtattg tgatgatctg cgatgaagtg
      301 atgagtggct tcgggcgcac tggcaaatgg tttgcaattg aaaattggaa tgtggtgcca
      361 gatatgatta ctatggcaaa gggacttaca tcggcgtatg ctccgctcgg ggctgtggca
      421 atgaagccag agattgctgc tgcgtttgac gagcatgcct ttgaaagcgg gctgacgtat
      481 acatctcatc ctatttcatt ggcggcggct gtggcgaaca tccaagcgat gagggaggat
      541 aaggttgtag aacatgcagc aagcatgggc ggagtcttga ggcgtatgtt atccgatttg
      601 ggtgaggcgc atccatctgt gggggaggtg cggaatatcg gtctatttgg tatcatcgag
      661 ttggtaaaga atcgtgagac gaaagaaccc atggcgccat ggaacgggtc ttcgccagaa
      721 atggttgcct tgaaaaaata ttgttccgat catggactgt ttctatatac ccattggcat
      781 acggtgttga tcatcccgcc gttgattatt agcgaaaaag aattgcagga aggctttgat
      841 gttttggatg aggcattgaa gattacagat aggacttcgg atggataact tcataccgag
      901 tttgaagtaa aattttagaa atcaaaaagg agaatgtcta atggaatccc agcccttcaa
      961 ctcttcccct gctcccgccc ctgcttctgg tgggaatgaa cgtgtgatgg ctatcgcttc
     1021 actcgtgatt ggcgtcatta atttgtgcgc atggtttttg cctatctgtg gcgcaccact
     1081 tggcataatt ggtattgtgc ttggtatctt cggtatgaag gatccttcac agaagacctt
     1141 ggcaattatt ggtattgcct taagcgcaat tggtttgctt gcggcttgta tcaatggtgc
     1201 tgtgggcgct tatatgggtg ttacggggca gggttttaat ttctctccgt aattctttgt
     1261 caggcgttga aaaaagctct tgcaatatgc aagagctttt ttttatggct gtgttgtatt
     1321 ggcgggttgc ggcatgcgcc tgccgcctgg aaagatgggc atttgggcaa tagaatatgc
     1381 ggcaaagtaa gcggtatcgg ctccggcttc accagggagc ggttcaatac gattgttacc
     1441 atttgtatct tcgccatata gtaagaggct tgaataggtt tctatttctg caatggttga
     1501 ttccataccg gtgccatagg gcatactctg aagtttaagc gctacttcaa gtagttgttc
     1561 gctccagcct tccatatttt gcagacaaac aattaagttt tgtccgcttt ggatgatgcc
     1621 tgtggtcgca tcgggagcat ctatagcaaa ttctgtatgg ctgatcgtca aggggatgta
     1681 gccttgttct gtgccatttg caagtagccc aaacccatca ttggggtcgt tgatctcacc
     1741 atctccattc cagtctttat acaactgaag gttttcttct cctacaagtt gattaataat
     1801 ttcttcattc tttattcgca gagttgcttc atcgccttca ttgaatgcat ctctaagctc
     1861 taaaacggat gtatcgattg tatcggctgt gtcccatacg ccttggatga gggctgttcc
     1921 attgggcgtc atgcctatgg agacaagaat atgccttata tggacaaatg cctgcggagg
     1981 gaacaccgaa gaggcggcaa cttcctggct gggttggtcg gggttgggat cattatccgg
     2041 ctctaccgtg accatcatct catcaaactg actgagaaga ttcgcaccgt ttgagtctga
     2101 gaaaattaat tgtccatttt gtccggtaaa tatcaccgga cccgcattta gttttatttc
     2161 tccgccttga cccaggaacc agacttcaaa atgttgtccg gctggtggta gggggattcc
     2221 gctgatggta actgtggcat tatcgaccaa ttcattgtaa tcatcaaaga caacggagcc
     2281 aatgggttga cttggattgg gtagttgatt gttattttgg gatggtacgt
//
LOCUS       DAOQNJ010000460         2330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_36568_length_2330_cov_9.067692, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000460 DAOQNJ010000000
VERSION     DAOQNJ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36568_length_2330_cov_9.067692"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..915)
                     /locus_tag="PLG52_11605"
     CDS             complement(142..915)
                     /locus_tag="PLG52_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42138.1"
                     /translation="MRLFWKGTATVFFYTISGALLIYAATRSLDFITATLPPDQQIIG
                     YLGLAATSGGLLAWLMVFMYKAEGIGQKITAGIMTALDMLGEFGLFTMDTLYQSGKSG
                     MTAQLTPDEIRLVVLSLSGLIALNIFATIIFHLLDSENMRRMRESFVRDQLEQNALKE
                     IEKRGEEIALTLAPALAKQWADEFERKFSDLKSLGLGTVTEDKTPAPGQSQPPAQPKN
                     PPIQPQEAQKTTHEAPEAEIIPVPLALQGFSGNGNGHKK"
     gene            complement(920..1246)
                     /locus_tag="PLG52_11610"
     CDS             complement(920..1246)
                     /locus_tag="PLG52_11610"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNB42139.1"
                     /translation="MKIFFILPLLALACTLTAAPAQTTAEAIKPAQLAQVIIAPSPTQ
                     AACTVTALKTLNLRAKPGTSSAVIAILKHGDPLTILPDPPQGNWIHVQTQDRAGWINS
                     NYCKGQ"
     gene            complement(1249..1461)
                     /locus_tag="PLG52_11615"
     CDS             complement(1249..1461)
                     /locus_tag="PLG52_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42140.1"
                     /translation="MTPNQRREAGKRGGLATLAKHGKDHFSKIGKRGAATFWKRYKFE
                     PTGLNDFAIVKRETREVIAFLSGRPF"
     gene            complement(1568..1702)
                     /locus_tag="PLG52_11620"
     CDS             complement(1568..1702)
                     /locus_tag="PLG52_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42141.1"
                     /translation="MDKKLSELLRIWNQLNEQNKKDLLDYMRKAIERQNNQNIQKRIA
                     "
     gene            complement(2036..>2330)
                     /locus_tag="PLG52_11625"
     CDS             complement(2036..>2330)
                     /locus_tag="PLG52_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB42142.1"
                     /translation="RPSINDLTKGVIMLPAVFSNKAKKNSEMPDLAEYCTTLEAAKKL
                     GFHVKTIPGMMKNKELDGIRFGKVWLVSRKSVQEYLEKTKGMSKNDPRRKNKK"
BASE COUNT          485 a          552 c          607 g          686 t
ORIGIN      
        1 cgtgtccgtg gctattctct gggcggtctg cctttcgcaa tctgtacgcc agttcacggt
       61 ggcttcggtt gcaatagcgt tgagagtatg agccatcgaa ttcaagcccg caataagcgc
      121 atttccagcg gtagaaccgc tttacttttt gtgcccgttg ccgttgccgc tgaagccctg
      181 caaggctaaa gggacgggga taatctcagc ctccggcgct tcatgggtgg ttttttgggc
      241 ttcctgtggc tgaattgggg ggtttttggg ctgtgcaggc ggttgagatt ggccgggcgc
      301 gggtgtttta tcctctgtga cagttcccaa gcccagcgat ttgagatcgc taaacttgcg
      361 ttcgaattca tccgcccatt gtttagccag ggcgggggcg agtgtgagcg cgatttcttc
      421 gccgcgtttc tcgatctctt tgagcgcgtt ttgttcgagt tgatcgcgca caaatgactc
      481 gcgcatcctg cgcatgttct cactgtctag caggtggaaa atgatcgttg caaagatgtt
      541 caatgcaatc aatccgctaa gtgaaagcac aacaagccga atttcatcgg gtgtaagttg
      601 ggcggtcatt cctgatttgc cgctctggta gagtgtatcc atcgtgaaaa gcccgaattc
      661 ccccagcata tcaagcgcgg tcataatgcc cgctgtgatc ttctgcccga ttccctcagc
      721 cttgtacatg aaaaccatca accaggccaa aaggccgccg ctggtggccg ccagcccaag
      781 ataaccaatg atctgctgat cgggcggaag tgtggcggtg ataaagtcca gggatcgcgt
      841 ggcggcatag atcaatagtg cgccgctgat cgtgtagaaa aatactgttg ctgtgccttt
      901 ccaaaataat ctcatggtct tattgtcctt tgcaatagtt tgaatttatc catcctgcgc
      961 ggtcctgcgt ttgtacgtga atccaattgc cctggggcgg atccggtagg atggtcaatg
     1021 gatcgccatg tttcaaaatg gcgatgacag ccgaagatgt tccaggcttt gcacgtaggt
     1081 tcaaagtctt tagagctgtg acggtgcaag ccgcttgtgt gggtgatggg gcaatgatga
     1141 cctgcgcgag ctgcgcgggc ttgatcgctt ctgcggtcgt ttgagcaggt gcagcggtaa
     1201 gggtgcaggc gagtgccagc aggggaagta tgaaaaatat tttcatggtt agaacggcct
     1261 ccccgataaa aacgcgatga cttcgcgtgt ctcacgtttg acaatggcaa agtcattcag
     1321 cccggtgggt tcaaacttgt accgcttcca aaacgtagcc gcgccgcgtt tgccaatttt
     1381 ggaaaagtga tctttgccat gcttcgccag cgtagccagt ccgccgcgct tgcctgcttc
     1441 cctgcgttga ttgggtgtca tggttttgcg ttcctcctgt gtgatttgct ataatgacag
     1501 tggattgatt tgttgctggt caatcctcct gtgtccgccc ccgttctgat cgggggcggc
     1561 tatttattta tgcaatgcgc ttttggatgt tctgattgtt ctgtctttca atcgctttgc
     1621 gcatgtagtc gagcaggtct tttttatttt gctcattcag ctgattccaa atgcggagca
     1681 gttcagaaag tttcttatcc atgtttcatc acctcctttc agggttcgag ttttacttgt
     1741 ctttcaagaa tgaccaggct agtagggcac caagcaagcc gccagccgcg ccgataatga
     1801 caaagagcat cacgccgatt cacctccttt cacggtttgg attttttgcc gcgtaatcat
     1861 ccctgcgcga gtgcttcgcg tctggtttcg attagcggtt ataggtttgt taatgtgcta
     1921 tcaactttgg tagacagtaa atcgtaaaaa taaattcgcc tgcgcgaatt tttacagcct
     1981 actttggtag acagtgtatc aaaataaaaa acgcctgtca ataagcaaca agcgtttact
     2041 ttttgttttt gcgccgtgga tcgttcttgc tcatgccctt tgtcttttca agatattctt
     2101 gcacagactt tcgagagaca agccatactt ttccaaaacg tatcccatca agttctttgt
     2161 tcttcatcat gcctgggatt gtcttgacgt ggaatcctaa ttttttagcg gcttcgagcg
     2221 ttgtgcaata ctcagcgaga tcgggcattt ctgaattctt ctttgcctta ttagaaaaaa
     2281 cagcaggtag cattatgact cctttggtga gatcgttaat gctaggcctg
//
LOCUS       DAOQNJ010000461         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_36699_length_2325_cov_10.882379, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000461 DAOQNJ010000000
VERSION     DAOQNJ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36699_length_2325_cov_10.882379"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLG52_11630"
     CDS             <1..245
                     /locus_tag="PLG52_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42143.1"
                     /translation="FRQKQGMRDDLPLIPSLDDFYEAPQTLNRTVFTVVHDDAMVDKL
                     LEVTQTLVGNLNKPDTGIMVVTPVVRAYGLDPIDKK"
     gene            447..782
                     /locus_tag="PLG52_11635"
     CDS             447..782
                     /locus_tag="PLG52_11635"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNB42144.1"
                     /translation="MEHPTFLLLMAVVQEQDLDAATTALQKIGASSTYLSSAGGFLGR
                     RNATMLVGLPADKLQDSLKVLHEACRQRIEYMTLPLEGSPMPMPAPVPVTVGGATVFA
                     LPVERFEQI"
     gene            804..1064
                     /locus_tag="PLG52_11640"
     CDS             804..1064
                     /locus_tag="PLG52_11640"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNB42145.1"
                     /translation="MKMIIAIVHDNDADILTQAFTAASFRVTRVASTGGFLRSGVVTM
                     LLGVDDDQVDAVIETVRKALPSKGDKKRATVFVVPVSHFEQV"
     gene            1145..>2325
                     /locus_tag="PLG52_11645"
     CDS             1145..>2325
                     /locus_tag="PLG52_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42146.1"
                     /translation="MKNKKIFAFLALLLLALACANPLTTPAEQPANVETIVAATISAL
                     TFTPAPSTPVVGDSASSLLPHSIYYLAPDSAQITQVFRLEKDGKTITQLTFEPSKVED
                     FDVSLADGRIVFLTNHQLYLANADGSNRLLIFDAGPQDEVNMFLTRIISPVFSLSGQT
                     IAFGHQGLNFYSLTSGQTNRVLEDKVNDPGQGFIIPEELYWPEMYTTNENKLILTLGY
                     YEGASTGFYDISSGTLTRLDNEQRAIICCGDYSLSADSSSLYAGSPTFGMFAAGLWRV
                     DMNTGKVNPLLFGDYESDPVEVSDNPFIAPDGQLYYFYASIPNTGDMVSRPPLQLVRS
                     APDGVTGRTVLRPETFTAMNEALWAPDASFVIIANGSEQVYAGGEAQLYYTDGQKTMI
                     SL"
BASE COUNT          604 a          620 c          525 g          576 t
ORIGIN      
        1 ggtttcgcca gaagcaaggc atgcgtgatg atcttccgct catcccttcg ttggatgatt
       61 tctacgaagc accgcaaacc cttaaccgca cagtctttac cgttgtccat gatgatgcca
      121 tggtcgacaa actcctcgaa gtcactcaaa cgctggtcgg taacttgaac aaaccagata
      181 ccggcatcat ggtcgtcacg ccggttgtcc gcgcctatgg gcttgacccc atagataaaa
      241 agtgatcgtt cataaaaagc agccacagag tttgcattct gtggctgctt tttcatattg
      301 cataggttct aggttgaagc atcccccgag tgatgataat atcaaagcag agataaaatc
      361 gtcgaaacag aatcaagccc gggcgccgaa agaagcgcaa ggtctaaaat ccaattcaat
      421 caaatcttga ttgaaggaga agagtcatgg aacacccaac ctttttatta ttgatggcag
      481 tggtgcaaga acaagacctc gatgccgcca ccacagccct gcaaaagatc ggcgcatcat
      541 ccacctatct ttcgagtgca ggcggtttct taggcaggcg caatgccacc atgttggtag
      601 gcttacccgc cgataaactg caagactcat tgaaggtctt gcacgaagcc tgtcgtcagc
      661 gcatcgagta catgacactc cccctcgaag gttcgcccat gcccatgcct gcaccggtgc
      721 ccgttacagt gggtggcgca accgtctttg cattgcccgt ggaacgcttc gaacaaattt
      781 agaatcaaag aggagaacct cccatgaaaa tgatcatcgc catcgtacac gataatgacg
      841 ccgatatcct aactcaagcg tttactgctg ccagcttccg tgtgactcgt gtggcatcta
      901 cgggcggctt tttgcgcagc ggggttgtca ccatgctcct cggcgttgat gacgaccaag
      961 tggacgctgt gattgaaact gtccgcaagg cgttgccatc caaaggggat aaaaaacgcg
     1021 ccactgtttt tgttgtgcct gtcagtcatt ttgaacaagt gtaaagcgtg tataaacaaa
     1081 gacgttgtgt cagcaggaac attccagccg gtacaacgtc tttgttttgt ttaacgaggt
     1141 gattatgaaa aacaaaaaga tctttgcgtt ccttgctctt ttattacttg cccttgcatg
     1201 cgccaaccca ttgacaacac cagccgaaca accagccaac gtggaaacga tcgttgccgc
     1261 aaccatttct gcattgacgt tcactccggc tccttccacg cccgtagttg gtgattccgc
     1321 atccagcctt ctgccacact ccatttatta tcttgctcct gactctgccc agatcacaca
     1381 agtctttcgt ttggaaaaag atggcaagac cattactcaa ctcaccttcg aaccctccaa
     1441 agtagaagac ttcgatgttt ctctcgcaga tggcagaatc gttttcctca ccaatcatca
     1501 gctgtacctt gcaaatgcag atggcagtaa ccgcctcctg atcttcgatg caggtccgca
     1561 agatgaagtc aatatgtttc taacgcgcat tatcagcccg gtcttttctc tcagtggaca
     1621 aaccattgcc ttcggtcacc aaggcttgaa tttttactcc ctcactagcg gacaaaccaa
     1681 ccgcgtgctg gaagataaag tcaacgaccc tgggcagggc ttcatcattc cagaagagtt
     1741 gtactggccc gaaatgtata caaccaatga aaacaaattg atcctcacat tgggatacta
     1801 cgaaggcgcc tctactggat tttatgacat cagcagcggc acattgactc gacttgataa
     1861 tgaacaacga gcgatcatct gttgcggaga ttactcactg agcgctgatt cttccagttt
     1921 atatgcaggc agccccacat ttggtatgtt tgccgccggt ctttggcgcg tggacatgaa
     1981 caccggcaaa gtaaaccctt tgctatttgg agattatgag agtgacccgg tcgaagtcag
     2041 cgataatcca tttatcgcgc cagatggtca gctgtattat ttttatgcca gcattccaaa
     2101 cacaggggat atggttagcc gtccgccact gcaactcgtc cgctcggcac cagacggtgt
     2161 aacaggtcga accgttctcc gcccagaaac atttactgcc atgaacgaag cactctgggc
     2221 acctgatgcg agtttcgtca tcatcgccaa tggaagtgaa caagtctatg ctggcggcga
     2281 agcacagctt tactatacag acggtcagaa gaccatgatt tcgct
//
LOCUS       DAOQNJ010000462         2324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_36724_length_2324_cov_9.223887, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000462 DAOQNJ010000000
VERSION     DAOQNJ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36724_length_2324_cov_9.223887"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLG52_11650"
     CDS             <1..371
                     /locus_tag="PLG52_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42147.1"
                     /translation="NLYLRKPMPTKTPSIISTVLTVLSLLVFVIASLFFSLVALNGVS
                     ESQGTPALIASLICNGIGIILAAVVAWRMPAWLIHKFSWKSLWAVLVSLIVSFFLGGG
                     ISIVAMFVSILVADILWNAR"
     gene            518..>2324
                     /gene="glgP"
                     /locus_tag="PLG52_11655"
     CDS             518..>2324
                     /gene="glgP"
                     /locus_tag="PLG52_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNB42148.1"
                     /translation="MEFLTSPSQKFDLPKRIERLGELATNLWWSWHPEAAHLFGRLDY
                     DLWERLGHNPIRILNEVGRTRLNQASKEKEYLELYDAVFKTFDAYFSKETWSQKTHKE
                     LKKPIAYFSMEYGLHETLPIYSGGLGVLSGDHLKEASDLGLPFIAVGFMYAQGYFSQR
                     ITEDGWQEALNNPLNFDHLPVSLVTKDGKAVTVSFDFPDRKLTAQIWEVRVGRVPLYL
                     LDSNVESNSEYDRLLTARLYWSDLDRRIMQEVLLGIGGARAIRALGYDPAVWHMNEGH
                     ASFLTLERIREHVASGSTFTAAIDKTRGQNIFTTHTPVPAGNDEFPLYLIDKYLSTYW
                     SELKLTREEFIDVAKHNHPQGDMYSMPVLALKLSGGSNGVSELHGEVSRDMWQFLWND
                     RSIKDVPIQHVTNGVHTRNWMARRMNNLLEKHLGADWYDNLDDHALLEKIDQIPDNEL
                     WGVHLHLKRKLSFYLKERVRDRWTRGGFHPVQVVASGVLINPYALTIGFARRFATYKR
                     ASLVLSDVERLLELINRPNMPVQIIFAGKAHPADDPGKKLIQQVYRTVKKAETGGRIV
                     FIEDYEMNLARYLVQGVDVWMNTPRRPYEASGTSGM"
BASE COUNT          553 a          662 c          532 g          577 t
ORIGIN      
        1 ataaccttta tttgaggaaa cccatgccta ccaaaacccc gtccattatt tcaactgtgc
       61 taacggttct aagcttgctt gtgtttgtga tcgccagctt gttcttttct ttggttgctc
      121 tgaatggggt ttcagaaagt cagggcaccc ccgcgctgat cgcatcgctg atatgtaatg
      181 ggatcggaat catcctcgcc gccgtcgtcg catggagaat gcctgcctgg ctgatccata
      241 aatttagctg gaaaagcctt tgggcagtgc tggtttcctt aatagtcagc ttcttccttg
      301 gcggagggat ttctatcgtt gccatgttcg tcagcattct cgtagcggac atattatgga
      361 atgcccgtta aacctgaatc aacattttgc tcaaatgacg gtcatcttta caggtgaccg
      421 tcatttttaa ttccgaagtt catttagttt gaccaaacaa ctaatccgcc ttacaatcgc
      481 tggacgcaaa aaaacaatga ggtatcatcc gcccactatg gaattcttaa cttctccctc
      541 tcaaaaattc gatttgccaa aacgtatcga acgtcttggt gaacttgcca ccaacttgtg
      601 gtggtcctgg catcctgaag ctgcgcatct ctttggtcgt ttggactatg acctgtggga
      661 acgactcggg cacaacccca tccgcattct taatgaagtt gggcgcacac gcttaaatca
      721 ggcatcgaaa gaaaaagaat atctcgaact atatgatgcc gtcttcaaaa ctttcgatgc
      781 atactttagc aaagaaacct ggtcacaaaa gacgcacaaa gagttgaaaa aaccgattgc
      841 ctatttctcg atggaatatg gcttgcatga aaccctgccc atttattctg gtggcttggg
      901 cgtgctttca ggggatcatc tcaaagaagc ctcagacctc ggcttgcctt tcatcgcagt
      961 cggcttcatg tatgctcagg gatatttctc ccaacgcatc actgaagacg gctggcagga
     1021 agctctcaac aacccgctca acttcgatca cctgcccgtc tcccttgtca ccaaagatgg
     1081 caaagccgtc accgtttcat tcgattttcc tgatcgaaag ctcaccgcgc aaatttggga
     1141 agtgcgcgtt gggcgtgttc cgctctactt gctcgactca aacgtcgaaa gcaacagcga
     1201 atatgaccgt ctgctgaccg cccgcctcta ctggtctgac ctagatcgcc gcatcatgca
     1261 ggaagttctg ttgggaatcg gcggtgcgcg cgccatccgc gccttgggat atgaccccgc
     1321 cgtctggcac atgaatgaag gtcacgcctc tttcttaacc ctcgaacgga ttcgtgaaca
     1381 cgtggcgtca ggctccacct ttaccgccgc catcgataaa acccgcggac agaacatctt
     1441 caccactcac actcccgtcc ccgctggcaa cgacgaattc ccgctctatc tcattgataa
     1501 atacctctcc acctactgga gcgaactgaa gctcacccgc gaagagttca tcgacgttgc
     1561 caagcacaac cacccacaag gtgacatgta ttccatgcct gtcttggcgc tcaaactctc
     1621 gggcggctcg aacggcgttt ctgaattaca tggcgaagtc tcgcgcgata tgtggcaatt
     1681 cctttggaat gaccgcagta taaaagatgt gcccattcaa cacgtcacca acggtgttca
     1741 cacccgcaat tggatggcac gccgcatgaa caacctgctc gaaaaacatc tcggcgcaga
     1801 ttggtacgac aacctcgacg accacgcctt gctggaaaag atcgaccaga ttccggataa
     1861 cgaactttgg ggcgttcacc ttcacctcaa acgcaagctt tccttctatc tcaaagaacg
     1921 cgtccgcgac cgttggactc gcggtgggtt tcacccggtt caggtcgtcg cttcgggcgt
     1981 gcttatcaac ccttatgcgt tgaccattgg cttcgcccgc cgtttcgcga cctataaacg
     2041 tgccagcctc gtcctttcgg atgtagaacg cttgttggag ctgatcaacc gcccgaacat
     2101 gccggtgcag atcatcttcg cgggcaaggc acacccagct gatgaccccg gcaagaagct
     2161 cattcaacag gtctatcgca ctgtcaagaa agctgagact ggtggtcgca tcgtcttcat
     2221 tgaagattac gaaatgaacc tcgcccgcta cctcgtccaa ggtgtggacg tgtggatgaa
     2281 tactccgcgc cgcccttacg aagcgagtgg aacctccggc atga
//
LOCUS       DAOQNJ010000463         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_36780_length_2322_cov_6.658139, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000463 DAOQNJ010000000
VERSION     DAOQNJ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36780_length_2322_cov_6.658139"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLG52_11660"
     CDS             complement(<1..605)
                     /locus_tag="PLG52_11660"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB42149.1"
                     /translation="MLQRLRENKSMQGTFLALPTTLWLFVLLIIPLFLTLVVSFGKRS
                     PDGDVIYSFGLHNYIRLIGYSTDCPDGQSACFDPLYVNILWRSLTLAFNTTAIVIMLA
                     YPLSYFIARSHPNRRNMFLFMVLIPLWTNFVIRVYAWMMLLRKEGAINIIIGWVANLL
                     HIPFTPLEMLYTPGAVLVGMVYEFLPFMILPIYTSLEKIDNS"
     gene            complement(608..1723)
                     /locus_tag="PLG52_11665"
     CDS             complement(608..1723)
                     /locus_tag="PLG52_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB42150.1"
                     /translation="MTSEFAVEMRDVVKKFITPEGNELNAVDHVTMQIKNGEFFSMLG
                     SSGCGKTTSLRMIAGFEWPTEGEVYIEGKPMGHTPPFQRKVNTVFQSYALFQHMTVFQ
                     NIAFGLEMEGAKEEEIKKRVGRALEMVQLTGMERRRPKQLSGGQQQRVAVARALVKTP
                     DVLLLDEPLGALDLKLRKEMQLELKALQQQLGITFIYVTHDQEEALTMSDRIAVMSKG
                     RVQQLATPVEIYERPVNKFVADFIGESNFLDGKIKSISKDEAVVFVPALNTELKGMPM
                     SKDFVNGEEVAVSVRPEKIRIVENESKGDGLFKAKVLTSVYIGSDTHVYVDMEGIRLK
                     VMEQNRISRLDPSSFYSKDQVVTLLFMPENTLVLKKD"
     gene            complement(1796..>2322)
                     /locus_tag="PLG52_11670"
     CDS             complement(1796..>2322)
                     /locus_tag="PLG52_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB42151.1"
                     /translation="WGVEHWNVVPDILTVAKSLGGGVMPISAVTTTEEIFHPMMYPNP
                     FMHTTTTGGGALACSAAISAIHVTLREKLWEQAAEKGAYLMPKLENFVVQYPSIFEKV
                     TGKGLLIGMHFKNPEIGYKVASGLFKRGVLVAGTLTSAQTVRIEPPLVVTYPQMDALL
                     DRLDEVLKEINKSM"
BASE COUNT          646 a          579 c          515 g          582 t
ORIGIN      
        1 gagttatcga tcttctcaag cgaggtgtag atcggcagaa tcatgaacgg caaaaactca
       61 tacaccatgc ctaccaagac cgcgcccggc gtgtataaca tttccaaagg tgtgaatgga
      121 atgtgcagca agttcgcaac ccatccgata atgatgttaa tagcgccttc cttgcgaagc
      181 aacatcatcc aagcataaac gcggatcacg aagttcgtcc atagaggaat caacaccata
      241 aaaagaaaca tattcctgcg gttcgggtgt gaacgagcaa tgaaatacga aagcggatac
      301 gccaacataa ttacgatggc agtggtgttg aaagccaacg tcaatgaacg ccacaaaatg
      361 ttgacgtaca gcgggtcaaa gcaagcagac tgtccatctg ggcagtcggt gctgtaaccg
      421 atcaagcgaa tgtaattatg taacccaaaa gaatagatta catcaccatc tgggctgcgc
      481 ttaccaaaac taaccaccaa tgttaggaat aagggaatga tcagcagcac gaacagccat
      541 aaagtcgtgg gaagcgcaag gaatgttccc tgcatcgatt tgttttcacg taaacgctgt
      601 aacatggcta atcctttttc aagacaagcg tattctcagg catgaacaat aatgtaacca
      661 cctgatcctt cgaatagaac gaggatgggt cgaggcgtga aatgcggttt tgttccatca
      721 ccttcaaacg aatgccttcc atgtccacat acacatgggt atcagagcca atgtacacag
      781 aagtaagtac ctttgccttg aagagaccat cacccttgga ttcattctct acgatacgaa
      841 tcttttctgg gcgaaccgaa acagcaactt cctctccatt gacgaagtcc ttcgacatgg
      901 gcatgccctt caactccgta ttgagcgccg ggacaaaaac caccgcctca tctttcgaga
      961 tggacttgat cttgccatcc aggaagtttg actcgccgat aaagtctgca acgaatttat
     1021 tgaccggtcg ttcgtaaatt tcaactggtg tagcaagctg ctgaacacgt cccttgctca
     1081 tgacggcaat acggtcagac atggtcagag cttcttcttg gtcgtgtgta acgtaaatga
     1141 atgtgatgcc aagttgctgt tgcaaagctt taagttcaag ctgcatttct ttgcggagtt
     1201 ttaagtccaa tgcgccgagc ggttcatcaa gcagaagcac atctggcgtc ttaaccaaag
     1261 cacgcgcaac tgctacacgt tgttgctgac caccagaaag ctgcttgggg cggcggcgtt
     1321 ccatgcctgt gagctgaacc atctccaatg cacgtccgac gcgtttcttg atctcttcct
     1381 ctttggcgcc ttccatctcg agaccaaaag caatgttctg aaacaccgtc atatgctgga
     1441 acaaggcata actttgaaag acagtattca ctttgcgttg gaaaggtggc gtatgcccca
     1501 tgggcttgcc ttcaatatag acctcccctt ctgtgggcca ttcaaagcct gcgatcatac
     1561 gcagggacgt agttttgcca cagcctgagg aaccaagcat tgagaagaat tcaccgttct
     1621 tgatctgcat cgtgacatga tcgacggcgt tcaattcatt cccttctggc gttatgaact
     1681 tctttacaac atcacgcatc tcgacagcaa attcactggt catctatctt ctccgtcatg
     1741 aaataataga cttccgaaat tcggggagtg tttcccgatc tttcggaagt atgaattaca
     1801 tagatttatt gatttctttc aacacttcat ccaagcgatc taacaaagca tccatttgtg
     1861 ggtaggttac gaccaaaggt ggttcaatgc gaacggtttg agcgctggtc agcgttccgg
     1921 caaccaagac accacgcttg aaaagaccag aagccacttt atatcctatt tcaggatttt
     1981 tgaagtgcat gccaatcaac aagcctttgc ctgtcacttt ttcaaaaatg cttgggtatt
     2041 gtacaacaaa attttccaat tttggcatca ggtaggcacc tttttcagct gcctgctccc
     2101 aaagcttttc acgcagagtg acatggatgg ctgagatcgc cgccgaacaa gccaaagcac
     2161 caccacccgt tgtggtggtg tgcataaacg gattcggata catcatcgga tggaagattt
     2221 cttcggtggt agtcaccgcg ctgataggca tgaccccacc gccaagtgac tttgccacgg
     2281 tcaaaatatc agggacgaca ttccaatgct caacgcccca ca
//
LOCUS       DAOQNJ010000464         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_37069_length_2312_cov_10.369960, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000464 DAOQNJ010000000
VERSION     DAOQNJ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37069_length_2312_cov_10.369960"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..997)
                     /gene="pstC"
                     /locus_tag="PLG52_11675"
     CDS             complement(47..997)
                     /gene="pstC"
                     /locus_tag="PLG52_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNB42152.1"
                     /translation="MKEETLHIKPLEAPRFRQFDLKKKFRLSESVIQGLLFFAGILSI
                     LTTIAIVYELGKEAMLFFNDPDVTFAKFFGTTQWQPGIGQYGIWALVSASLTTSAIAM
                     SISLPLGLASAVYLSEYASPRVRATLKPILEILAGIPTVVYGYFALLFVTPILRNIFG
                     EDLSVYNMLSAGLVMGIMILPLISSMSEDALSAVPRALREGAYAMGATKLETSLQIVL
                     PAALSGIGAAFIIGISRAVGETMIVAIAAGSGPNFSFNPLEAAETMTGHIARISGGDL
                     SYNSIDYNSLFSIALMLFLVTLVLNLISQWVVNRFRERYE"
     gene            complement(1093..2163)
                     /locus_tag="PLG52_11680"
     CDS             complement(1093..2163)
                     /locus_tag="PLG52_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB42153.1"
                     /translation="MSKIVRMSLFLMVALSLVLAACGAPAEEAPATESATEVVATEAA
                     TEEPTAVATEDPMAAFAPDAVEGDVITAGSSTVFPLSERMAELFQDEGFAGNVTVDSI
                     GTGAGFERFCTTGETDISNASRAIKDSEKEACAAIGREPIEFRVGTDALAVVVSSSND
                     FVTDLTKEQLGKIFVGEYTTWDQVDPSFPAEAIQIYSPGSDSGTFDYFIEAVAAKVLP
                     KVNDKDDLKGAEEKLLGLAGAQFSEDDNVLVKGIEGSPYAIGYFGYAYYAEEAGNLKA
                     INVDGVAPNQEDVDNGTYPLARPLFIYSDAKIMQEKPQVAAFIYFYLSNVNDNISDVG
                     YFPAPAEALDASMAAWEAAVGQ"
BASE COUNT          597 a          555 c          634 g          526 t
ORIGIN      
        1 agtacagaat agaacgcttc gccttgtgga tatgaaggtt gtttgctcat tcgtaacgct
       61 ccctgaagcg attgaccacc cattggctga tgagattcaa cactaatgtg acgaggaata
      121 acatcaatgc aatggagaac aagctgttgt aatcaatcga gttataggac aagtcaccgc
      181 cgctgatgcg cgcgatgtgt ccggtcatgg tttcggcggc ttcgagcgga ttgaacgaga
      241 agtttggtcc agacccggcg gcgatggcga cgatcatcgt ctcaccgacg gcgcgggaga
      301 taccgatgat gaatgccgct ccgatcccag agagagccgc aggtaatacg atctgcaaac
      361 tggtttcgag cttggtcgcg cccatggcat aggcaccttc acgcaaggca cgcggaacgg
      421 cgctcaaggc atcttcgctc atggaagaga tcaacggcag gatcatgatg cccatcacca
      481 acccggcaga aagcatgttg tagaccgaaa ggtcttcgcc gaagatgttt ctgagaatag
      541 gggtgacaaa gagcaaggca aaatatccat acaccacggt ggggatgccc gccaagattt
      601 caagaatcgg cttgagtgtg gcgcgaacac gcggggaggc atattcgctc aagtacacgg
      661 cagatgccaa ccctaacggc agggagatgg acattgcaat tgcactggtg gtcagtgagg
      721 cagagaccaa tgcccaaatg ccatattgac caataccggg ctgccattgg gtggtgccga
      781 aaaattttgc gaaggttaca tcagggtcgt tgaaaaagag catagcttct tttcctaatt
      841 catacacgat ggcgatcgta gtcaaaatag acagaatgcc agcgaagaac aacaatccct
      901 gaatgacact ttcactaagc ctgaatttct tcttcaggtc aaattgacgg aagcgcggcg
      961 cttccaatgg tttgatatgt aaagtttctt ctttcataac atttctctga aacaagattt
     1021 cataaaaagg agaatacacg caaatgggct ggtctccatt tggagaccag ccctgcatta
     1081 tttgacgttc aattactgtc caactgcggc ttcccaggct gccattgaag catcgagcgc
     1141 ttcggcaggg gcggggaagt agcccacatc agagatattg tcgttgacgt tgctcaagta
     1201 gaagtagatg aacgcagcaa cctggggctt ttcctgcatg atcttggcat ctgagtagat
     1261 gaagagcggg cgggcgagcg ggtaggtgcc gttgtctacg tcttcctgat tcggggcgac
     1321 gccgtcaaca ttgatcgcct ttaagtttcc ggcttcttct gcataataag catagccgaa
     1381 gtaaccgatc gcgtaggggc tgccttcgat tcccttgacg agaacgttgt catcttcaga
     1441 gaactgggca ccagcaagac cgagcaattt ttcttcagcg cctttaaggt catccttgtc
     1501 attaactttg ggcagtactt tggcggctac ggcttcaatg aagtagtcga aggtgccaga
     1561 gtcagagcca ggggagtaga tttgaatggc ttcggcgggg aaagaggggt caacctgatc
     1621 ccaagttgtg tattcaccga cgaagatctt gccaagctgt tccttggtga ggtcggtgac
     1681 gaaatcattg ctggagctca cgaccacggc aagggcatcg gttccaacgc ggaactcaat
     1741 tggttcgcga ccaatggcag cgcaagcttc tttctcgctg tccttgatgg cgcgggaagc
     1801 attggaaata tcggtttcac cggtggtgca gaagcgttcg aaaccagcgc cagtaccaat
     1861 gctgtcgacg gtcacgttac cggcgaagcc ttcatcctgg aacaattccg ccatgcgctc
     1921 agagagcggg aacacggtgg aggagccagc ggtgatcaca tcaccttcaa cggcgtcggg
     1981 ggcaaatgca gccatcgggt cttcggtggc aacagcggtg ggttcttcgg ttgcagcttc
     2041 agtggcaaca acttcggtag cggattcagt agcaggtgct tcttcagcag gcgcaccgca
     2101 ggcggcaagc acgaggctca aagccaccat gaggaaaagg gacatacgaa cgattttgga
     2161 cattctctaa attctccttt gaatttgtac gttcgtgatg ttatcaaccg gctcttaagg
     2221 tcaatggaag gatgggttaa catcttgtta agaacaaaga ggcaatcagc catcatcaaa
     2281 atccgttaca attaacctat gaacaaatcc cc
//
LOCUS       DAOQNJ010000465         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_37450_length_2299_cov_6.807487, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000465 DAOQNJ010000000
VERSION     DAOQNJ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37450_length_2299_cov_6.807487"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..741)
                     /locus_tag="PLG52_11685"
     CDS             complement(295..741)
                     /locus_tag="PLG52_11685"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNB42154.1"
                     /translation="MDFSSINWLAVLFCVLASMVIGGVWFGPKTFFPIWWKAVGKAGE
                     PSMGGGENTPGSSLGMGMVWGGVVFAAFVESVFMSMMVHAMGSISGGVTLASGALTGL
                     LIWLGFVASSSLTNKLFADRLQAWVLEQGNHLITFVVMGAIVGIMG"
     gene            complement(897..>2299)
                     /locus_tag="PLG52_11690"
     CDS             complement(897..>2299)
                     /locus_tag="PLG52_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNB42155.1"
                     /translation="PESKEKRVFNNVLGAMGNTPLVKLERIGKDLPVPLYAKLEFMNP
                     GGSVKDRVGANIIEQAEKRGEIKPGGTIVEATSGNTGVGLAIAAALKGYKTIFVMPDK
                     MSNEKILLLRAYGAKVVITPTAVGPDDPRSYYEVAKKFARETPNAILANQYHNPDNPK
                     THELSTGPEIWEQTDGKVTDVIIGIGTGGTITGVGRYLKAKNPNITIVGVDIEGSILT
                     EIWQNKGIIPPGAYPKTYKVEGIGEDFLPSTMDIKYVDAIERAGDRESFLWARQLVRQ
                     EGIFAGGSSGSAIAGAIKYCRKLQPGRLPVVILPDSGSRYLSKFYDDKWMREFGFLSM
                     EFGETALGDLLIAKPNKALITATLGDSIKKVVTVMHQNAVSQMPVVGTDGALVGLIEE
                     VDLLNHMLEKHDHSNEEKIDPLIQNAGAVFPPETPLEDAMPSLTAGYALIVVENSRPI
                     GILTKIDVLDYVAGKI"
BASE COUNT          600 a          600 c          492 g          607 t
ORIGIN      
        1 ccccatgcgt ttcactaagt cagggatggc aaccagcatg gcattaaacc ataaggcggt
       61 gactgcatca aagtcttttt cttgatacgg acggatcata ttctctccaa taaaatacag
      121 cggacggtta accgtccgct gtattttact tcacggatgt tacgcagtag gggcgaccct
      181 atcgcgtttt gtgataactg ttagcaaagg gcgactcttc ctaataaatt ggcataaggt
      241 taagccttga cgggtcgccc ctacgatgaa atttcgtaag ggagttactt atacctagcc
      301 catgatgcca acaatggcac ccatcactac gaaagtgata aggtgattgc cttgttcaag
      361 cacccaagcc tgcaaacgat cggcgaacaa cttattggtc aggctcgaag atgccacaaa
      421 accaagccag atcaacaatc cagtgagcgc gccggaggca agtgtcactc cgccagagat
      481 actacccatg gcgtggacca tcatggacat gaacacagat tcgacgaagg cggcaaagac
      541 gaccccaccc caaaccatcc ccatgccaag tgaggagccg ggtgtgttct cgccgccacc
      601 catgctcggt tcacccgctt tgccaacagc tttccaccaa attgggaaga aggtcttggg
      661 tccgaaccaa acgccgccaa taaccatgct tgccagaaca caaaataaaa cagccaacca
      721 attaatagaa ctgaaatcca ttttctttct ccttgggacg aaaatttgta ctctcattat
      781 aatagaaaat atgttctaat caaattaagg aattttttca cataaccttt ccctaaaagc
      841 aaaaggagaa gctttaccgc ttctcctttt tgtttaagtt cttatttacc agtaggttag
      901 attttccctg ctacataatc caaaacgtca atcttggtaa gaataccaat gggtctgcta
      961 ttctcaacca caattaaagc gtagccggct gtaagtgaag gcattgcatc ctcaagcgga
     1021 gtttcaggtg ggaaaaccgc gcctgcgttc tgaatcaacg ggtcaatctt ttcttcgttg
     1081 ctatggtcgt gcttctccaa catatgattc agcaagtcca cttcttcgat tagcccaacc
     1141 aaggctccat ctgtacctac aactggcatt tgcgatacag cattctgatg catcactgtc
     1201 acaactttct taatcgagtc gcctagtgtg gcggtaatca aagccttatt cggtttcgca
     1261 atcaacaggt caccgagagc cgtttcgcca aattccatcg agaggaagcc gaattcacgc
     1321 atccatttat catcataaaa cttggaaaga taacgcgaac ccgaatccgg caagataaca
     1381 accggcaaac gtcccggctg gagtttgcgg caatacttga tggcacctgc aatcgcagaa
     1441 cccgatgagc cgcctgcaaa aatgccttcc tgtcgcacca gttgacgtgc ccacaagaaa
     1501 gactcacgat cccctgcacg ttcaatggca tcgacatatt taatgtccat ggttgagggc
     1561 aggaagtctt caccgatgcc ttctacttta taggtctttg gatatgctcc aggaggaatg
     1621 atgcctttat tctgccaaat ttcggtcaga atcgagcctt cgatatccac accgacaatt
     1681 gtgatgtttg gattcttggc tttgagataa cgcccaaccc cggtgatggt gccgccagtt
     1741 ccaatgccaa tgatgacatc ggtcactttg ccatcggtct gctcccaaat ttcaggtccg
     1801 gtgctcagtt catgagtctt tgggttatcg gggttgtgat attgattcgc taaaattgca
     1861 ttgggggttt cgcgggcaaa cttctttgct acttcataat aagaacgtgg gtcgtctggt
     1921 ccgaccgcgg tgggagtaat cacaactttg gcaccatatg cacgtaacaa taaaatcttt
     1981 tcgttgctca tcttatcggg catcacgaag atggttttat agcccttcag tgctgccgca
     2041 attgccaacc ccacccctgt attcccagag gtcgcttcga cgatggtgcc cccaggtttg
     2101 atctctccgc gtttttctgc ttgttcaata atattcgccc ccaccctatc tttgaccgac
     2161 ccgccgggat tcataaattc cagtttggca tatagcggaa caggcaggtc ttttccaatt
     2221 cgttccagtt tcacgagcgg ggtatttccc atcgctccaa gtacattgtt gaatacacgt
     2281 ttctctttgg attctggca
//
LOCUS       DAOQNJ010000466         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_37619_length_2293_cov_9.735925, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000466 DAOQNJ010000000
VERSION     DAOQNJ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37619_length_2293_cov_9.735925"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1119)
                     /gene="pseB"
                     /locus_tag="PLG52_11695"
     CDS             complement(109..1119)
                     /gene="pseB"
                     /locus_tag="PLG52_11695"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HNB42156.1"
                     /translation="MDWKNQVVLVTGGTGSFGKKFTRILLEEKQPKKIIIFSRDELKQ
                     HEMQVGGYNDPRLRYFIGDVRDRERMVRAMHGVDIVVHAAALKQVPACEYNPMEAIKT
                     NIMGTANVVEAALDAGVKKVLTVSTDKAVSPANLYGATKLAAEKLTIQSNAYAGGSAT
                     RFSCVRYGNVVGSRGSVVPLFLKLRSTGKVTITDDRMTRFWLSLEQGVRFVIDSIEQM
                     EGGEVFVPKIPSTKVTDLAKAIAPDAKLEIIGIRPGEKLHEMLISEDEARHTIELDKM
                     YVVQPAEATWFGYSWKDKGKELKEGFSYSSDNNTEWLDVEGIKKYIAPFEELFAQGKL
                     EG"
     gene            complement(1206..2000)
                     /locus_tag="PLG52_11700"
     CDS             complement(1206..2000)
                     /locus_tag="PLG52_11700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB42157.1"
                     /translation="MSKLTDKEYLTQKQYKDSTNLDVRVAIHKRFSTNSYGWFNWVFD
                     SFSALPENATILEIGCGTGELWKECESRIPKNWALTLTDISDGMLDAAWRNLIRIHRG
                     IKFEKVDSQSIPYADKTFDAVIANYMLYHVADRKKALAEIKRVLKDDGVLFSATAGAN
                     HLREMYEWVFRVSGGRHGQIALQFTLENGKEQLQEFFPRVELSRYPDSLQITDVDMLI
                     AYVRSMASVELSEEEIQKLEQEWSEILVKDGAIHISKDAGLFQALQ"
     gene            complement(2000..>2293)
                     /locus_tag="PLG52_11705"
     CDS             complement(2000..>2293)
                     /locus_tag="PLG52_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42158.1"
                     /translation="FQAADLAAQTGDWERVAALGDEAIALSFAPEDRVEWLPFLKAYA
                     IRGDAETLKQLSKRVIGEKIIRLQACEMLSSIEQPLNDDVREMIDVNYCKGSE"
BASE COUNT          596 a          587 c          454 g          656 t
ORIGIN      
        1 cgttcatcat ttcttgtgcg aaattgatgc cgagtaatcc gcttgcacct gtaagtagaa
       61 tcttcatctt cctgcctcag ttcgttttat ccaaacaaat acagagaatt agccttccaa
      121 ctttccttgt gcaaatagct cttcaaacgg agcaatgtat ttcttgatgc cttctacatc
      181 gagccattcg gtgttgttgt cgcttgaata agagaaacct tctttcagct ctttgccttt
      241 gtctttccat gaatagccaa accaagtcgc ttcggcaggc tgaaccacat acatcttatc
      301 aagttcaatc gtatggcgtg cttcatcttc tgagatgagc atttcatgca atttttcacc
      361 ggggcgaatt ccaataattt caagcttggc atctggcgca atcgctttgg caagatccgt
      421 cactttggtg ctggggatct tcggcacaaa gacttcgccg ccttccattt gttcgatgct
      481 gtcaatcacg aaacgcacgc cttgttcaag cgaaagccag aaacgggtca tgcggtcatc
      541 tgtaattgtg actttgcctg tagaacgcaa tttgaggaag agaggaacaa ccgagccgcg
      601 actaccaacg acattcccgt atcggacgca agagaagcgg gttgccgagc ccccagcata
      661 agcattactc tgaatcgtta acttctccgc tgcgagtttg gtagcaccat ataaattcgc
      721 cgggctgacg gctttatccg tgctgacggt gaggactttc ttcacgcctg catccaaagc
      781 ggcttcaacc acatttgctg ttcccatgat attggttttg atggcttcca tcgggttgta
      841 ctcacaagca ggcacctgct taagcgccgc cgcatggacg acaatatcaa caccatgcat
      901 ggcacgcacc atgcgttcgc ggtcgcgcac atcgccaatg aaatagcgca gacgcgggtc
      961 gttatatccg cctacctgca tttcgtgctg tttcagctca tcacgactaa agatgatgat
     1021 cttctttggt tgtttctctt cgagcaaaat tcgggtgaat ttcttgccga aggaaccagt
     1081 cccaccggta acgagcacta cttgattttt ccagtccatg aaatctcctt gtttcggtaa
     1141 aacaggctta caacctgcta agacctgtta ttctaaatta ttaagcaact tgcccattaa
     1201 caattctatt gtagagcctg aaatagcccg gcatctttag aaatatgaat tgcgccatct
     1261 tttactaaaa tttcagacca ctcttgttca agtttttgta tttcttcttc cgaaagttcc
     1321 acagaagcca tcgagcggac atatgctatg agcatatcaa catccgtaat ctgcaaacta
     1381 tctggataac gagataattc tacacgtgga aagaactctt gcaattgttc ttttccattt
     1441 tcgagcgtga attgcaatgc aatctgccca tgtcgcccgc cactcacacg aaaaacccac
     1501 tcatacattt ctcgcaaatg gtttgcacct gcagttgcag aaaatagcac gccatcatct
     1561 ttcagaacac gtttaatttc agccaatgct tttttacggt ctgcgacatg atagagcatg
     1621 taattggcaa tcaccgcatc aaaggtttta tctgcatacg gaatggattg cgaatccact
     1681 ttttcaaatt tgataccacg atggatgcgg atcaaattac gccatgctgc atccaacatt
     1741 ccatcagaaa tatccgttaa cgtgagcgcc caatttttgg ggatgcggct ttcacactct
     1801 ttccaaagtt cacctgtgcc acaacctatt tccaaaatgg tagcattctc gggcaaagcg
     1861 ctaaaagaat caaataccca attaaaccat ccatacgaat ttgtactaaa tcgtttatgg
     1921 atcgcgaccc gaacatccaa gttcgtagag tctttatatt gcttctgtgt cagatattcc
     1981 ttgtccgtaa gtttggacat cactcagaac ctttgcaata gtttacgtcg atcatttcac
     2041 gcacatcatc atttaatggt tgctcaatgc tgctcaacat ttcacacgcc tgtaagcgga
     2101 taatcttctc gccaatcacg cgtttggata attgtttcaa tgtttctgca tcaccacgaa
     2161 tggcatatgc tttcaaaaaa ggtaaccatt ccacgcggtc ttcgggggca aagctcaaag
     2221 cgatagcttc atctccgaga gcagcaaccc gctcccagtc cccggtttga gccgcaaggt
     2281 ccgctgcttg gaa
//
LOCUS       DAOQNJ010000467         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_38139_length_2275_cov_4.723874, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000467 DAOQNJ010000000
VERSION     DAOQNJ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38139_length_2275_cov_4.723874"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLG52_11710"
     CDS             <1..350
                     /locus_tag="PLG52_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNB42159.1"
                     /translation="ALGAKGKILDASEHDRILAATSHLPFLISSALTLATPEEVAPFV
                     GPGFKSTSRLAGTSSSMMLGVLQSNRENVLEALHRMQNEFAQLEAALTAGDFVKVESL
                     LNKAQAKHQSFTQ"
     gene            347..1648
                     /gene="aroA"
                     /locus_tag="PLG52_11715"
     CDS             347..1648
                     /gene="aroA"
                     /locus_tag="PLG52_11715"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNB42160.1"
                     /translation="MNIHHSSFRIHPLSHPLSATVRVPGSKSLTNRALLISSLANGTT
                     HLTNTLFSDDSHYFAKALQTLGFNVQLDEENFSMTVTGMGGKIPARKAELFIGNAGTA
                     ARFLSAFLTLGHGEYILDGEPRMRERPIRDLIDSLSQLGAKLEPTNDCPPVKISANGL
                     PGGKTTIAGDISSQFLSALLMVAPYAKSPIEVTLSTDLNSKPYVDMTISIMKDFGVEV
                     ERDGYEYFKVPVTSYQLPVTSYPIESDASAASYFFAAPAICGGTVTVENISHKSVQGD
                     VAFVDVLKQMGCAVEEHDNSILVTRASSLVGIDVDMRDIPDTAQTLAAIAPFASSPTR
                     IRGIASARVKETDRVHATCVGLDGLGVRVEEHEDGMTIYPVEKMRSASIQTYNDHRMA
                     MAFSLIGLRFDGVTIENPSCVSKTFPNYFEVLEELRTQGNK"
     gene            1656..>2275
                     /locus_tag="PLG52_11720"
     CDS             1656..>2275
                     /locus_tag="PLG52_11720"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNB42161.1"
                     /translation="MMNYQLGLIGYPLGHSLSPKIHTAALVACGLQGTYSLFPIHPDN
                     KQGLHNLLAQVRAGEIRGLNVTIPHKQNVIEFMDELTPTAKAIGAVNTIYMRADKLIG
                     DNTDAPGFLSDLKRVSNSSFSTPLSALVLGAGGSARAVVYALANDGWNVTLAARRVEQ
                     AQQLASSIDNYQLSIVNYSNLQPSTFDLLVNTTPLGMTPNTEASPLP"
BASE COUNT          574 a          641 c          481 g          579 t
ORIGIN      
        1 aagcgctggg agccaaaggt aaaatccttg acgcctcaga acatgaccgc atcctagccg
       61 cgacgagtca tctgcctttc ttgatctcat ctgcgctgac attggcaacc cctgaagaag
      121 tcgctccctt tgtcgggcct ggcttcaagt ccacgagcag acttgctggc acatcttcct
      181 ccatgatgtt gggcgtttta cagtccaacc gcgagaatgt gttggaggcg ttgcatcgaa
      241 tgcaaaatga atttgctcaa ctcgaagccg cactaaccgc tggcgatttt gtaaaagtcg
      301 aatcccttct aaacaaagcg caagccaaac accaatcttt cactcaatga atattcatca
      361 ttcatcattc cgcattcatc ctttgtctca tcctctctcc gccaccgtcc gcgtacctgg
      421 ctccaagtca ttgaccaacc gcgccctgct gatttcgtcc cttgcaaacg gcacgaccca
      481 cctcaccaat acactcttct ccgacgactc gcattacttc gctaaagccc tgcaaaccct
      541 cggctttaat gttcaattgg atgaagaaaa tttttccatg acggtcacgg gcatgggagg
      601 gaaaatccct gccaggaaag ccgaactctt tatcggcaat gcgggcactg cggcaagatt
      661 tctctccgcg ttcctcacac ttgggcatgg cgaatacatt ttggatggcg aaccgcgcat
      721 gcgcgaacgc ccaataaggg atttgattga ttcgttgtca caacttggcg caaaactcga
      781 accgacaaat gactgtccac ccgtaaagat ttcagcaaat ggcttgcctg gtggaaagac
      841 aaccattgct ggtgatattt cttctcaatt cttatccgca ttgctgatgg tcgctcctta
      901 tgctaaaagc cctattgaag tcactctctc cactgatctc aactctaagc cctacgtaga
      961 catgacaatt tccatcatga aagacttcgg tgtggaagtt gaacgggatg gatatgaata
     1021 tttcaaagtg ccagtgacca gttatcagtt accagttacc agttatccaa ttgaaagtga
     1081 cgcctccgct gcgtcctatt tcttcgccgc ccctgccatc tgcggcggga cagtcacagt
     1141 agaaaacatc tcgcacaagt ctgtgcaagg cgatgttgct tttgtagatg tgctaaaaca
     1201 aatgggttgt gctgttgaag agcacgataa ttcgatactc gttactcgtg cctcgtcgct
     1261 tgttggcatt gacgtagata tgcgcgatat tcccgacacc gcccaaactc ttgctgcgat
     1321 cgccccattt gcttcatcac caaccagaat tcgtggcatc gcatctgcca gagtcaaaga
     1381 gacagatcga gtccacgcga cctgtgttgg gttggacgga ctcggcgtcc gcgttgaaga
     1441 gcatgaagat ggaatgacca tctatcccgt agaaaagatg cgcagcgctt ccatccaaac
     1501 ctataacgat catcgcatgg caatggcatt ctctctcatc ggcttacgct ttgatggtgt
     1561 caccatcgaa aatccatctt gtgtttcaaa gacatttcct aattactttg aagtgcttga
     1621 agaattgcgt acccaaggaa acaagtaaac aggtgatgat gaattatcaa cttggtttaa
     1681 tcggttatcc cttgggacac tcactatcgc caaagattca tacagcggct ttagtcgctt
     1741 gcggcttaca aggaacctat tccctattcc ctattcaccc tgacaacaaa caaggcttgc
     1801 acaacctgct cgcccaagtt cgtgctggcg aaattcgcgg actcaacgtc accattccgc
     1861 acaaacagaa cgtcatcgaa ttcatggacg aactcacgcc caccgccaaa gccatcggcg
     1921 cggtcaatac gatttatatg cgtgcagata aactcattgg cgataatacc gatgcgccag
     1981 ggtttctctc tgacctcaag cgggtttcaa attcatcatt cagcactccg ctttctgcat
     2041 tggtcttggg tgcaggtggt tctgcgcgag ccgtagttta tgctttagcc aatgatggct
     2101 ggaatgtcac ccttgccgcc cgtcgcgtcg aacaagccca acaactcgca agttcaattg
     2161 ataattatca attatcaatt gtcaattatt caaaccttca accatcaacc ttcgacctgc
     2221 tcgtcaacac caccccgctc ggcatgacgc ccaacaccga agcctccccc ttgcc
//
LOCUS       DAOQNJ010000468         2269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_38332_length_2269_cov_7.425474, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000468 DAOQNJ010000000
VERSION     DAOQNJ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38332_length_2269_cov_7.425474"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLG52_11725"
     CDS             <1..1009
                     /locus_tag="PLG52_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42162.1"
                     /translation="LSIAGGLIPVILVNRHVTLPDYSRYTLIASIGAVMLLALLIESI
                     SKRGTQITVMGVLVSVAVLTHYGNTIRYVDENQATRNFWWQVAWRAPKIEEGVTLLAM
                     YPNSPLSEDYFIWSPANFIYYPEKQSNNPVMVKLPAAVLDGNTVTQVTTNGGVETPLR
                     RGNYLERDFGNVLVMVQSSPNSCVRFINGASPELNAFDDGRVVQVAPYSKLNAVSTDG
                     SIPVVPENILGSEPERGWCFYYQQADLARQRGDWDSITILLDEALAKGYYPNDPLEWI
                     PFMQAYAVQEDVDEMREMTKLIVLDRYLRLQVCNNMKYLAENESLSAEVNEFITKKIC
                     E"
     gene            1061..2008
                     /locus_tag="PLG52_11730"
     CDS             1061..2008
                     /locus_tag="PLG52_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNB42163.1"
                     /translation="MPTVLMTAPYMIPFLDRFRPVLAEYGIDLIVPDVQERMEEEDIL
                     KYAGQFDGTICGDDRYTARVIEACLPRLKVISKWGTGVDSIDGEACAKFGVKLGRTPN
                     AFTTPVSDTVLGYMLAFARRGPWMDREMKSGKWEKIPGKTLSECTLGVIGLGNIGKAV
                     TRRAKAFGMKVYGTDIIEIDHVFVSETGIEMTTLEYLLSNSDFVSVNCDLNPTSHHII
                     NSDTLALMKSTAVLINTARGPLVDEKALVAALSSGQIGGAALDVFEFEPLPAESPLLK
                     MDNVMLAPHNSNSSPTAWERIHWSTIKNLVEGLGMPYKK"
     gene            2005..>2269
                     /locus_tag="PLG52_11735"
     CDS             2005..>2269
                     /locus_tag="PLG52_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42164.1"
                     /translation="MMNKLRLALPYIFLLLLTAFVLDLVNPSFDVPARDGGFFLYAGQ
                     QILNGKIPYQDFWDSKGPGIFYVNAFGLLLGGGSRWGVWAVEFI"
BASE COUNT          535 a          501 c          641 g          592 t
ORIGIN      
        1 cttgagcatt gcgggcggac tcatcccggt catcctcgtc aatcggcatg tcaccctgcc
       61 ggattattca cgttatacct tgatcgcttc aattggcgcg gtgatgctgt tggcgttgct
      121 gatcgaaagt atttcaaaac gtggaacgca aatcactgtg atgggtgtgt tggtgtctgt
      181 tgcagtgctg acccattacg gcaataccat tcgctatgtg gatgagaatc aggcgacgcg
      241 taatttttgg tggcaggtgg cgtggcgtgc gccgaagatc gaagaaggcg tgacgctgct
      301 ggcgatgtac ccgaatagcc cgttgagcga agattatttt atttggagcc cggcgaattt
      361 tatttattat ccagaaaaac aaagtaacaa cccggtgatg gtgaaattgc ctgctgccgt
      421 gttagatgga aatacagtca cgcaagtgac aaccaatggc ggagtcgaaa ctccgttgcg
      481 acgcggaaat tatctggagc gtgattttgg caatgtgctg gtgatggtgc agtccagccc
      541 gaatagttgt gtgcgtttta tcaatggcgc ttcgccagag ttgaatgcgt ttgacgatgg
      601 acgtgtggtg caggtggcac catactcaaa attgaatgcg gtatctacgg atggtagcat
      661 tccagttgtg ccagagaata ttctcggcag tgagccggaa cgtggttggt gtttttatta
      721 tcaacaggca gaccttgccc gccagcgcgg agattgggat tctataacaa tcttactcga
      781 tgaagcttta gccaaaggtt attacccgaa tgatccgctt gaatggattc cgtttatgca
      841 ggcgtatgcg gtgcaagagg atgtggatga gatgcgcgaa atgacgaaac tgatcgtgct
      901 cgatcgatat ttgcgtttgc aggtttgtaa taatatgaag tacctcgctg aaaacgaatc
      961 gctttctgct gaggtgaatg aatttataac gaagaaaatt tgtgaataac actccgctct
     1021 ggtgggggct tagcccccac cagagcgagg agaaggtaac atgccaactg ttttgatgac
     1081 tgccccttat atgatcccat tccttgaccg tttccgcccg gtgctggcgg agtacggcat
     1141 tgacctgatc gtgccagatg tgcaagaacg catggaagaa gaagatattc tcaagtatgc
     1201 cggtcagttc gatggcacca tatgtggtga tgaccgttat acggcgcgag tgatcgaggc
     1261 ttgtctgccg aggttgaagg tcatctcaaa gtggggaacg ggagtcgact caatcgatgg
     1321 cgaggcttgt gcgaagtttg gtgtgaagct tgggagaacg ccgaatgcat tcaccacccc
     1381 cgtctcagat accgtgctgg gatacatgct cgccttcgca aggcgtggtc cgtggatgga
     1441 tcgcgagatg aaaagtggca agtgggagaa gattcccggc aagaccctca gtgaatgcac
     1501 actcggagta attggcttgg ggaatattgg caaagccgta acgcggcgcg ccaaagcctt
     1561 tggcatgaaa gtctatggca cggatatcat cgagattgac catgtctttg tcagtgaaac
     1621 cggaatcgaa atgacaactc tcgaatatct actatcgaat tccgattttg tctctgtcaa
     1681 ttgtgacctc aacccaacat cccatcacat tatcaattct gatacgcttg cgctaatgaa
     1741 atccactgcg gtgctcatca atacggcgcg cggtccactg gtggatgaaa aagctttggt
     1801 cgcggcgctc agctcaggtc aaatcggagg cgcggcactg gatgtcttcg agttcgagcc
     1861 gcttccggcg gagagtccgc tgttgaagat ggataacgtt atgcttgcgc cgcataattc
     1921 caactccagc cccacagcct gggagcgcat ccattggagc acgatcaaga atttggtcga
     1981 ggggttgggg atgccgtata aaaaatgatg aacaaacttc gccttgccct tccgtatatt
     2041 tttttgttgc tgctgacggc attcgtcctc gaccttgtca acccgtcgtt tgacgtgccc
     2101 gcccgcgatg gtggattctt tctgtatgca ggtcaacaaa ttttgaatgg caaaattcca
     2161 tatcaagact tttgggattc gaaaggtccc ggcatttttt atgtcaatgc gttcgggttg
     2221 ttgctgggcg gcgggtcacg ttggggcgtg tgggcagtag agtttattt
//
LOCUS       DAOQNJ010000469         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_38420_length_2266_cov_10.455902, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000469 DAOQNJ010000000
VERSION     DAOQNJ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38420_length_2266_cov_10.455902"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PLG52_11740"
     CDS             complement(<1..839)
                     /locus_tag="PLG52_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNB42165.1"
                     /translation="MNRLKNIDKRWLILAVVVVIIGIVLFPPVRNRLSVQYELLRTRI
                     VYFFNPPSEAVFQPSGEEEPLTVETAVGTVRAEMLLTLTPEATSTPKSTKTGPTAKPT
                     VTSTPVPGKVILPGVVYVDQHGRWNYCGPANLTMALKFWGWNGDRDDVAKVVKPGSPN
                     QNLDFVERGLPDKNVMPYELVDFVNLQTTEYRALSRYGGDMDLLKRLLAAGFPVIVEK
                     GYYERDYTGKVDWLGHYLFTTGYDDERGGFIVQDAYLKPGKDLLSKYDEYQDGWRSFN
                     YLF"
     gene            1010..1519
                     /locus_tag="PLG52_11745"
     CDS             1010..1519
                     /locus_tag="PLG52_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42166.1"
                     /translation="MRKSTLFVSAMLTVFLMATMFGVVSAYQQVVKNNDLASTQSSQK
                     ALSQPVSVEFAAPATATVVAPLVVTAEEATTIAVNFLNDPMVYSVEVVDYQGAPTYLV
                     TFSSGELVYVSSEGVVVANTQVQPVVITASNTTSRGQSNNSGSSSNNSSGSNQQHEEH
                     EHEEHEGDD"
     gene            1531..1923
                     /locus_tag="PLG52_11750"
     CDS             1531..1923
                     /locus_tag="PLG52_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42167.1"
                     /translation="MAPKPNTNKWSDVQLAIAAISMTSVIAFWNMFAGPDKVKADQKA
                     AEQQADLIPPTVMPVVVEPTTAPSVTMPPIGYTILFGGKAPQPKVVVVQKPKGGGGGG
                     NADSGNGGGGGGGGNAPASQPSTSTTSS"
     gene            1962..>2266
                     /locus_tag="PLG52_11755"
     CDS             1962..>2266
                     /locus_tag="PLG52_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNB42168.1"
                     /translation="MLVILQSDSKSAQDVLKDVPNWFEEWEQSLSRFRLDSELSRLNR
                     TFDQPVSVSNALWEVFQTAVWANGFSDGLVTPTVLDAMLDAGYDLSFNELSHEQFGM"
BASE COUNT          636 a          578 c          511 g          541 t
ORIGIN      
        1 atgaagaggt agttgaacga acgccagcca tcttgatatt catcgtactt gctcagcaag
       61 tctttgccag gtttgagata agcatcttgt acaatgaagc cgccacgttc atcatcatac
      121 cctgtcgtga acagataatg cccaagccaa tcgaccttgc cagtgtaatc gcgctcgtaa
      181 tatccctttt ccacgatcac agggaaacca gcagctagaa ggcgtttgag cagatccatg
      241 tcacccccat agcgggacaa ggcacggtat tcggtggtct gcaaattgac aaagtccacc
      301 aattcatagg gcatcacatt tttatcgggc aaaccacgtt caacaaaatc gaggttttga
      361 ttcggtgaac ccggcttgac gacttttgca acatcgtcgc gatcgccatt ccaaccccag
      421 aatttcaatg ccatcgtcaa attagctgga ccacaataat tccaacgtcc atgttggtct
      481 acatacacca cacccggcaa aatgaccttc cccggcaccg gcgtcgatgt aaccgtcggt
      541 ttggcggttg gacctgtttt ggttgacttc ggggttgagg tcgcttcagg ggttaacgtc
      601 aacaacatct ctgcgcggac ggttccgact gcggtttcaa ccgtcagcgg ttcttcttca
      661 ccgctgggtt gaaaaacagc ttcactaggc ggattaaaaa agtagacaat gcgtgtgcgt
      721 aaaagttcat attgaactga aagtcgattt cgcacgggtg ggaagagcac aataccaata
      781 atgaccacaa caaccgcaag aatcaaccaa cgcttgtcaa tatttttaag tctattcata
      841 aaaaagatta taaagcaaaa caaagaccgt tttttgcttc ccgttcgtca tactgacaat
      901 ttctacaaag tcctttaatg ccaaactcat ttaactgcat aaccttttcc ctgctgaaac
      961 gacttgactc aaagtaacag gttgaaaccc aatctgtaag gagtaaatta tgagaaaaag
     1021 cactttgttc gtttcagcca tgctcaccgt atttctaatg gcgaccatgt tcggcgtggt
     1081 ttctgcgtat caacaagtcg ttaagaacaa tgacttagcc tctacacaat cttcacaaaa
     1141 agccttatca cagccagtca gcgttgaatt tgcagcccca gcaacggcaa cggtcgtcgc
     1201 gccacttgtt gtaacggcag aagaagcaac aaccattgcc gtcaatttct taaatgaccc
     1261 catggtatac agcgtggaag tagtggatta ccagggcgca cctacttatt tggtgacctt
     1321 tagctcaggt gaattagttt atgtcagctc cgaaggcgta gtggtagcga atactcaagt
     1381 gcaacccgtt gtgatcactg catccaacac caccagcaga ggtcaatcga acaacagcgg
     1441 aagttcttca aacaactcaa gcggaagcaa tcaacaacat gaagagcacg aacatgaaga
     1501 acacgaaggc gacgattaat aggaatcccg atggcaccca aacccaatac aaataaatgg
     1561 tctgatgttc aattagcaat cgccgcgatc tcgatgactt ccgtcatcgc attttggaat
     1621 atgttcgcag gtccagataa agtaaaagca gatcaaaaag cagcagaaca acaggcggat
     1681 ctcatccctc ccaccgttat gcccgttgtc gtagagccca ccactgcgcc atctgtaacc
     1741 atgcccccta ttggatacac catcttattc ggcggaaaag cacctcaacc caaagtcgtt
     1801 gttgtgcaaa agccgaaagg aggcggaggc ggcggtaacg ctgattcagg aaacggcgga
     1861 ggtggtggcg gtggcggaaa cgcaccagcc agtcagcctt ctacatcgac cacttcatcc
     1921 taatgtttta cagccatccc tttcgcgcga tgggcagtga catgctcgtc attcttcaat
     1981 cggattcaaa gtctgcacag gatgttttaa aggatgtgcc gaactggttc gaagaatggg
     2041 agcaatccct ttcccgcttc cggctggaca gtgagctgtc tcgcctgaac cgaacctttg
     2101 accaacccgt gagcgtgagc aatgcgctct gggaggtctt tcaaacagcc gtgtgggcaa
     2161 atgggttcag tgacgggctg gtcacaccca ccgtgttaga tgcgatgtta gatgcaggct
     2221 acgacctttc attcaatgaa ctatcgcatg aacagttcgg catgtt
//
LOCUS       DAOQNJ010000470         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_38464_length_2265_cov_8.471041, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000470 DAOQNJ010000000
VERSION     DAOQNJ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38464_length_2265_cov_8.471041"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLG52_11760"
     CDS             <1..299
                     /locus_tag="PLG52_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42169.1"
                     /translation="VKVIMTWDIAAERDQEYFEFIIGEFVPGVQRLGLQPAEAWATLY
                     GSYPQIQVGLLATDAPEARRVLASAEWAMLQDRLFGYVKNYSYKVVPVRTGFQF"
     gene            362..>2265
                     /locus_tag="PLG52_11765"
     CDS             362..>2265
                     /locus_tag="PLG52_11765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB42170.1"
                     /translation="MPNKFSKILPWFFFAAAFQSLLAVAALLRIPSEGLSLARLALLG
                     AIGGIFLLSIGLGLYARRNLLRFEWLSTTPVIISSALLSLTFSLLLFLLRYFNPERFL
                     PYYERMSPLLWLLFFLAAEAALCFLLLKNGFHPQSLTNYSSAFRASVIPLLVLFTIFL
                     FVAITKIGVTPDTGYWGEPGVAIQGWHFVIALLLGCTVLILSSSNSSLFIRYLPFVLY
                     AIAAILWLSVPLETLANSFYVSTTPPTNMPLPYSDAGFYDFSAQSLLIGTGYFGGIPP
                     RPLYVVFLSILHLVFGQNYPAVINAQVLVLAFFPVALYFLAKKLHSPAAGVTVALFAI
                     FREYTGLWIASNTRVANSKVFTTDFPTAFAIVIICLVILWWLERRDLRSTLIAGGAFG
                     IFLLFRSQSMLTIPFLFLLVLLVFNFKWGEWIKSGIIFAVTMLLAVIPWLTHNYTIAG
                     KFSFDDPNQVAVIYSQYAFTGNLDLSQFNPETDSVRERIISFTLENPAYVANFITSHF
                     LNTEIGGLLALPLIKPFNGLQEPVNLYWIEWDGNLEWYNVILVLFYLAIVALGVGSAW
                     HRMKWMALVPLAFNLGYAVSNGIARFSSWRYNMPIDWVFYFYFALGIVEVFSILAQLF
                     GAQTKETQTET"
BASE COUNT          473 a          560 c          481 g          751 t
ORIGIN      
        1 cagttaaagt catcatgacc tgggatatcg ccgctgaacg cgaccaggaa tattttgaat
       61 tcatcatcgg tgaattcgta cccggtgtgc aacggcttgg cttacagccc gccgaagctt
      121 gggctaccct ttacggttca tacccgcaaa ttcaagtagg cttgcttgcc actgatgcac
      181 ccgaagcgcg ccgtgtgttg gcatctgctg agtgggcgat gttgcaagac cgtttgttcg
      241 gctatgttaa aaactattct tataaagttg tacctgtaag aactgggttt caattttaga
      301 taccaataca ccatcattgc gaggagggtg atagctctcc cgcaatgacg gaataggtca
      361 catgccaaat aaattttcaa aaattctgcc gtggtttttc tttgccgcgg catttcagtc
      421 tctactcgcc gtcgctgcac tgttgcgaat tccatctgaa ggcttgtcgc tggcgcgcct
      481 tgccttgctt ggagcaatag gaggcatctt tttattaagc ataggattgg gactgtatgc
      541 acgccgcaac cttttacgct tcgaatggct ttcgacaaca ccggttatta tctcgtccgc
      601 gcttctctcg ctgaccttta gcctgcttct gttcctttta cgttatttca accctgagcg
      661 ttttctaccc tactacgagc ggatgagtcc gctgctttgg cttttattct tccttgccgc
      721 tgaagccgct ctctgcttcc ttctcctcaa aaacggattc catccgcaaa gcctcacaaa
      781 ctatagctcg gcatttcgcg cttctgttat tccgctttta gtccttttta ctatctttct
      841 tttcgtagct attaccaaga tcggcgtcac cccagatacc ggctactggg gcgaaccagg
      901 tgtagccatt caaggctggc attttgttat tgctcttctg ttgggatgca ctgttctaat
      961 actttcctcc tctaactcat cgctttttat tcgttacttg ccttttgttc tttatgctat
     1021 tgctgccatc ctttggttaa gtgttccgct tgaaacgttg gcaaatagct tttatgtctc
     1081 cacaacgcca ccgacgaata tgcctttacc atattccgat gctggttttt acgacttttc
     1141 tgctcaaagc ttgttaattg gtactggtta cttcggtggg attccgccac gcccattgta
     1201 tgtggttttt ctatccatct tgcatttggt atttgggcaa aattatccag ccgtgatcaa
     1261 tgcacaagtt ttggtattag catttttccc ggtcgcttta tattttcttg cgaagaaatt
     1321 gcactctccc gccgccggtg tgaccgttgc tctttttgcc attttccgcg aatacaccgg
     1381 tttgtggatt gcctcgaata cccgtgtcgc taactccaaa gtctttacca cagacttccc
     1441 aacagctttt gctattgtca ttatctgttt ggtcattctt tggtggctgg agcgaagaga
     1501 cttgcgttct accttaattg cgggtggagc atttggtatt ttcttgctct tccgttcgca
     1561 atctatgcta accattccat ttctgtttct tttggtcttg ttggtattca acttcaaatg
     1621 gggtgagtgg attaaatcgg gcattatctt tgctgtcact atgttattgg ctgtcattcc
     1681 atggctgacg cataactaca ccattgcagg caaattctca tttgatgacc caaaccaagt
     1741 ggcagtcatt tatagccagt atgcttttac aggcaacctt gacctcagcc aattcaaccc
     1801 tgagaccgat agcgtccgcg aacggattat ttcattcaca cttgaaaacc cggcgtacgt
     1861 ggcgaacttt attacctctc atttcctaaa tactgagatt ggcggtttgc ttgcgcttcc
     1921 acttatcaaa ccattcaatg gactgcaaga accggtcaac ttgtactgga tcgaatggga
     1981 tgggaacctt gaatggtaca acgtcattct tgtcttgttc tatctggcaa ttgttgcact
     2041 aggtgtaggc tctgcctggc atcgcatgaa gtggatggcg cttgttccat tggccttcaa
     2101 tttaggatat gcggtttcca atggcatcgc ccgtttttct agctggcgct ataacatgcc
     2161 aattgactgg gtcttttact tctactttgc ccttggcatt gtagaagtgt tcagtatttt
     2221 ggctcagtta tttggagccc agacaaaaga aacccaaact gaaac
//
LOCUS       DAOQNJ010000471         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_38610_length_2260_cov_7.553288, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000471 DAOQNJ010000000
VERSION     DAOQNJ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38610_length_2260_cov_7.553288"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..532
                     /locus_tag="PLG52_11770"
     CDS             41..532
                     /locus_tag="PLG52_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNB42171.1"
                     /translation="MNTPKRYHPILVTLHWLTVIFMLGAGFLSEEGGNSPINFHMILG
                     ALLLIVMVARLITRFSTARPAALDAGNAFFNKIGELTHLGLYAVAFAILGMGGAIAVK
                     RNLFGYLMDNTAQISRAGFLGDVHHLFYLMAMALIFLHVGAALYHQFMLKDNILSRMW
                     YGK"
     gene            615..1340
                     /locus_tag="PLG52_11775"
     CDS             615..1340
                     /locus_tag="PLG52_11775"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB42172.1"
                     /translation="MPSVQLFITCLTDSFFPETGEAVVDILSRLGISVDFTPEQTCCG
                     QPQFNAGLRNDARAIAEHTIKVFEKTRGDIVTPSGSCAHHFRHNLLELFEGDAEWYPR
                     AEAFGKRVFEFTEYLVDKLNVTDLGAVWDGLLTYHPSCHTLRGLNVDRQPRALLQNVK
                     GATLVQLPNEHECCGFGGVMSVEHPELSAEWLKRKISNLETTHAPTLVVTDAGCLMHI
                     MGGLKRQGKKQKVLHIAEILRNT"
     gene            1386..>2260
                     /locus_tag="PLG52_11780"
     CDS             1386..>2260
                     /locus_tag="PLG52_11780"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNB42173.1"
                     /translation="MNTFRNRIRQSIQNETLQTALDNNTERRLRGKTLAFESIPDWRE
                     RRQRAHRIRADVIDHLDEYLDQFVANAQTNGIIVHRAKDSKEAIEIVLKISQSITNHQ
                     LPNTLIAKSKSMVSEEIELNHTLEKHGINVVETDLGEYIVQLRKEKPSHLITPAAHLK
                     KEQVAQLFHEKLGIPYTEDIPTLTATARKVLREVFLTADIGISGVNFGVADTGGICIV
                     TNEGNGRMVTSIPKTHIALMGIERIAPNLDDLSLLLSLLPRSATGQKLTVYTQLIHKP
                     MPGQTRHIILLDNGRT"
BASE COUNT          601 a          622 c          477 g          560 t
ORIGIN      
        1 taatcatgca gttctatagt atgggcaagg agagcaaaaa atgaataccc caaaacgtta
       61 tcatcccatt ctcgttacgc tgcactggct tactgtgatc tttatgcttg gtgcaggctt
      121 cctttcagaa gaaggtggca attccccgat taactttcac atgattcttg gtgcgcttct
      181 tttgatcgtt atggttgcac gcttaatcac ccgtttttcc acggcgcgac ccgccgcatt
      241 ggatgcaggc aatgccttct tcaataagat cggtgagttg actcatcttg gattgtacgc
      301 agttgcattc gccattctcg gcatgggtgg agccattgca gtaaaacgca atctatttgg
      361 ttatcttatg gacaataccg ctcaaatcag ccgtgctggt tttcttggtg acgttcatca
      421 cctcttttac ctcatggcaa tggcattgat ctttttacat gtaggcgctg ccctctacca
      481 ccaatttatg ctcaaggaca atatcctctc gcgcatgtgg tacggaaaat agacattcca
      541 tagttttcct cttcaacgcc ccaacaaagt atgagctttg ttggggcgtt ttttattggt
      601 cgtataatcg acacatgccc agcgtccaac ttttcatcac ctgtctcacc gattcattct
      661 tccccgaaac cggtgaagcc gtcgtagaca ttctctcccg cctcggcatt agcgtggact
      721 ttacacccga acagacctgt tgcgggcagc cgcaattcaa tgctggctta cgcaacgatg
      781 cgcgcgctat cgctgaacac accatcaaag tatttgaaaa gactcgcgga gacatcgtca
      841 cgccatccgg ttcatgcgcc catcattttc ggcacaatct cttggaactt tttgaaggag
      901 atgccgaatg gtatccaagg gcggaagcat ttggcaagcg tgtctttgaa ttcaccgaat
      961 atttagtaga caagctcaac gtcaccgacc tcggtgctgt atgggacggt ctactcacct
     1021 atcacccatc ctgccacacc ctacgcggac tcaacgtgga cagacagcca cgcgctcttt
     1081 tgcaaaatgt caaaggcgca accttggtgc aactaccaaa tgaacacgaa tgctgcggct
     1141 tcggcggcgt gatgtctgtt gaacaccccg aactctcggc ggagtggttg aaacgcaaaa
     1201 tatccaacct tgaaaccaca cacgctccca cacttgtcgt caccgatgca ggttgtctca
     1261 tgcacatcat gggcggcttg aagcgacaag gaaagaaaca gaaagttttg catatcgcag
     1321 aaatattgcg taatacgtga aaagtaaaac gcgatatccc gtatctgatc tccaatacct
     1381 aatctatgaa tactttccga aatcgcatcc gtcaatccat ccaaaacgaa actcttcaga
     1441 cagcactcga taacaacacc gagcgccgtc tgcgaggaaa aactttagcc ttcgaatcta
     1501 ttccggattg gcgcgagaga cgccaacgcg ctcatcgcat ccgcgcggat gtgatcgatc
     1561 atctcgatga gtatttggat caattcgtcg ccaacgccca aaccaatggc atcatcgttc
     1621 atcgtgccaa ggattcgaaa gaagccatcg aaatcgtttt gaaaatttcc caatcaatta
     1681 ccaatcacca attaccaaac accctcatcg ccaaatccaa aagcatggtc tctgaagaga
     1741 tcgagctcaa tcacaccctc gaaaagcacg gcatcaacgt ggtcgaaacc gacttgggtg
     1801 aatacattgt tcaattgcga aaagagaaac caagtcacct catcacaccg gctgcgcatc
     1861 ttaagaagga acaagttgca cagctctttc acgaaaaact tggcatcccc tacactgaag
     1921 atattccaac acttacggct actgcgcgaa aagttttacg cgaagttttt ctcactgctg
     1981 atatcggcat cagcggagtc aactttggag tcgcagacac aggcggtatt tgcattgtca
     2041 ccaatgaagg caacgggcgt atggtcacga gcatacccaa aacgcatatt gcgttgatgg
     2101 gtatcgaacg tatcgcgccc aacctcgacg atctctcgct tctgctctca ctcttgccgc
     2161 gttccgccac cgggcaaaaa ctcacggtct acactcaact cattcacaaa ccgatgccgg
     2221 ggcaaactcg tcacatcatc ctgctcgata atggacgcac
//
LOCUS       DAOQNJ010000472         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_38835_length_2253_cov_13.464513, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000472 DAOQNJ010000000
VERSION     DAOQNJ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38835_length_2253_cov_13.464513"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /gene="uvrC"
                     /locus_tag="PLG52_11785"
     CDS             <1..1545
                     /gene="uvrC"
                     /locus_tag="PLG52_11785"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNB42174.1"
                     /translation="WNEPFPKITVTRQMEEDGSRYFGPYTSAWAVYQTLEVLRKIFPY
                     LTCDREITGNDPRACLYYDIKLCTGPCIGAVSKDGYRQMISDLMEFLSGHSEPIVERV
                     QADMLKASEEMRFEKAAALRDQLKAMQSIIERQKIVFGSDYADSDVIAMAREDGEACV
                     QIFFIRGGKLIGREYFILEGTEDTTDSQVMEEFVKQFYTEAANIPEQVMLPQELEERK
                     IIAQWLRSKRGGEKVELFVPNEGQPKDLVQMATENATETLSSLRAQWQADAHRQEQAL
                     SDLQTHLNLSAPPNRIECYDVSHTQGVATVGAMVVFEQGVPAKRLYRKFNIESTSIGN
                     PDDFASMEEMLTRRFRRWKGSQETESEVGSKKDEAFSFLPDLIIIDGGKGQLGRVVKV
                     MEQFEIFDKVPVVGLAKQEEEIFFPHKSDPLLLPRHSQGLYLVQRIRDEAHRYGITAH
                     RKKRSKLGLASQLDSIPEIGPTRRKALLKHFGSMDKIKEASVEELMTVKGMTETAAKN
                     VKAHLE"
     gene            1542..1922
                     /locus_tag="PLG52_11790"
     CDS             1542..1922
                     /locus_tag="PLG52_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB42175.1"
                     /translation="MKEVWIVDDDEEMARAVSLMLKLLDCTSTHYLGPRPAAQALLSG
                     KRPDMMILDINMPEVTGLDMLEFLRRRPEWKNLPVIMLSSEAADVMVDKALKLGADGY
                     VMKPVTIEELEKVMAQAFYKNIIK"
     gene            1932..2081
                     /locus_tag="PLG52_11795"
     CDS             1932..2081
                     /locus_tag="PLG52_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42176.1"
                     /translation="MAGKNSKNQKNNKVMEEKVAKRQKTAQILFAAFAILLIISMVLS
                     AVAKY"
BASE COUNT          645 a          517 c          577 g          514 t
ORIGIN      
        1 tggaatgaac cttttccaaa aatcacggtc acccgccaaa tggaagaaga tggctcgcgt
       61 tattttggtc catatacctc ggcatgggca gtgtatcaga cgctggaagt gctgcgaaaa
      121 atattcccct acttgacctg tgaccgcgag atcacaggca atgatccacg ggcatgtttg
      181 tattacgaca ttaagttgtg tacaggtccg tgcattgggg cggtttcaaa agatggctac
      241 cgccaaatga tctcagactt gatggaattc ctcagcggac acagcgaacc cattgtggaa
      301 cgcgtccaag cagacatgct caaagcatct gaagagatgc gttttgaaaa agccgctgcc
      361 ttacgagacc aattgaaggc gatgcaatcc atcattgaac ggcaaaaaat tgtcttcggg
      421 tcagattatg cggactcaga tgtcatcgcg atggcgcgcg aagatggcga agcctgcgtg
      481 caaatattct tcatccgcgg cggcaaattg atcgggcgcg agtacttcat ccttgaaggg
      541 acagaggata cgaccgatag tcaagtgatg gaagaattcg tcaagcagtt ctataccgaa
      601 gcagcaaaca tccctgaaca agtgatgttg ccgcaagaat tggaagaaag aaagatcatc
      661 gcgcaatggt tgcggtcaaa acgtggcggc gaaaaagtgg aattgttcgt gccaaatgaa
      721 ggtcagccca aagatttggt gcagatggca accgaaaatg ccaccgagac cttgtcctct
      781 ttgcgggcgc aatggcaagc cgatgcgcac cgacaagagc aggcgctttc tgatttgcaa
      841 actcatttga atttatctgc cccgccaaat cgcattgagt gttacgatgt gagtcacacc
      901 caaggcgtag caacggtcgg cgcgatggtt gtgttcgaac aaggtgttcc ggcaaaacgg
      961 ctgtatcgta agttcaacat tgaaagcacc agcataggaa acccggatga ctttgcatcg
     1021 atggaagaaa tgctcacccg tcgcttccgg cgttggaaag gctcacagga aaccgagtca
     1081 gaagtcgggt ccaagaaaga tgaggcgttt tccttcctcc cagacctgat tattatcgat
     1141 ggtggtaaag gtcaattggg aagagtcgtc aaagtaatgg agcaatttga gatcttcgat
     1201 aaagtgccgg ttgtgggtct cgccaaacaa gaagaagaga ttttcttccc gcacaaaagt
     1261 gacccgctgc tcctcccccg ccactcacag ggactctacc tcgtgcagag gattcgcgac
     1321 gaagcccatc gctacggaat tactgcgcat cgcaaaaaac gctcgaaact gggtcttgct
     1381 tctcaactgg attccatccc ggaaattggt cctactagaa ggaaggcgct cttgaaacat
     1441 ttcgggtcta tggataagat taaagaagcg agtgtcgaag aattgatgac agtcaaaggc
     1501 atgaccgaga ctgccgcgaa aaatgtaaag gctcatttag aatgaaagaa gtttggatcg
     1561 tagatgacga cgaagagatg gctcgcgccg tcagtttgat gctaaaactg ctggactgta
     1621 cctccacaca ctatctgggt ccgcgcccgg cagcgcaggc attgctctca ggcaagcgac
     1681 cagacatgat gatcttggac atcaacatgc ccgaagtgac cggcttggac atgctagagt
     1741 tcctacgccg ccgccctgaa tggaagaatt taccggttat catgctctcc tctgaagccg
     1801 ccgatgtgat ggtggataaa gccctcaagc tcggcgcaga cggatatgtg atgaaacctg
     1861 ttacaatcga ggaacttgag aaggtaatgg ctcaagcatt ctacaaaaat atcataaagt
     1921 gagtagttga aatggctgga aagaattcaa aaaatcaaaa aaataataag gtgatggaag
     1981 aaaaggtcgc taagcgccaa aagacggcac agattttgtt tgccgctttt gccatccttt
     2041 taatcatctc catggtcctc tctgcagtcg caaaatatta gcttggtttc attgtaagac
     2101 ccctttgcgc ggcgcctctt cgttatggtg ccgcgctttc ttgtgcaaat ttacaaaacc
     2161 gaccgcagac gatatcgtca agacttgagg aattatgtta gataaattac aagccattga
     2221 agaaaaattt gaacagcttg ggcaggaact gct
//
LOCUS       DAOQNJ010000473         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_38862_length_2252_cov_7.883022, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000473 DAOQNJ010000000
VERSION     DAOQNJ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38862_length_2252_cov_7.883022"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..526)
                     /locus_tag="PLG52_11800"
     CDS             complement(86..526)
                     /locus_tag="PLG52_11800"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNB42177.1"
                     /translation="MKTMATVPTPLPKQEVTRFARFLTVGAVGTLLDFSILTLLKLAG
                     MPTLFANSLSFTTGLLNNFTWNRLWTFGDTAKADWRKQLAQFTLVSLAGLALNNLIVL
                     SLEGVLGSLLGQPEWGYIPAKIIATGVIVFWNYFANRTWTFKNH"
     gene            676..2001
                     /locus_tag="PLG52_11805"
     CDS             676..2001
                     /locus_tag="PLG52_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB42178.1"
                     /translation="MHSTDDSTRILRTAAWIWLGFLLAMALMDIALYMPQVQNLLGDS
                     TRLQPALPNQPLPPTPIGNLPNSPLRGPHYPVFIFYTANGLTALFFLALTHWSDIQAK
                     MEDIYYPFLLSVISVAPIVINAFIVPRFPQGPLSNAEGMALRQLPVMFVALVLVAWNY
                     RFLHVVLFSIVTTFIELGLVAINPSDNRNITVYIFIAIIRTISFVAVGFFISFIVSRL
                     RKQRESLREANANLTHYASTLEQLTVSRERNRLARELHDTLAHSLTAISVSLETAKAY
                     FDIDLNQSREMIDTSLEATRKGVDETRRALKALRSSDLVDMGLRLAVKKAAESAASRF
                     SLDLELDLQDPMPSLSPDVEQTILRVTQEAIENMTKHSQAKKFSVRLTSDKHTTLVIQ
                     DDGIGFDMKPKDSTGHFGLVGMRERAELAGGTLKIESGKGKGTKVVLTI"
     gene            2059..>2252
                     /locus_tag="PLG52_11810"
     CDS             2059..>2252
                     /locus_tag="PLG52_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB42179.1"
                     /translation="MKILLCDDQAVIRDGLEMLLQLEKDFQVIGSAQDGFEAVELASK
                     KSPDLVLMDLKMPGMNGIEAT"
BASE COUNT          578 a          515 c          526 g          633 t
ORIGIN      
        1 gttgagggtt gaggttgaac aggtaaagtt gctgtagtca tttagtactc cttgtgaagc
       61 gctgcattat ttataagttt agcggttaat ggttcttgaa cgtccatgta cggtttgcaa
      121 aataattcca aaagacaatc acgcctgttg cgataatttt ggcagggatg tagccccact
      181 ctggttgacc tagaagactc cctaaaacac cttctagaga aagcacaatc aaattattca
      241 aagccaatcc tgccaaactg accaaggtaa actgtgccag ttgtttgcgc caatcggctt
      301 tggctgtgtc accgaaagtc cagaggcgat tccaagtaaa gttgttgagc agacccgtcg
      361 tgaatgagag ggagttcgca aacaaggtgg gcattcctgc cagtttgagc aaagtcagaa
      421 tgctgaaatc taacaaggtg ccaaccgcgc cgacggttaa gaagcgcgcg aagcgggtca
      481 cttcttgttt ggggagggga gtaggaacgg ttgccatagt tttcatgttg aatattttat
      541 tttccacccg agaaagcgca gagtgcccca aatcatgatt cctaaggatc acccatgaca
      601 cctgtcatgg gtggatttac aaggtcatta catgggtcat gcccatgaaa aatcccttca
      661 tgataagatg cagatatgca ttcaacggat gactccactc gcattcttcg cactgccgcc
      721 tggatttggc ttggctttct acttgccatg gcgctgatgg atattgcgct gtatatgcct
      781 caggtgcaaa acttgttggg agattcaact cgcttacaac ctgccctgcc aaaccaacct
      841 ttgccgccta cccccattgg aaatttacca aactctcctt tgcgtggacc acactaccct
      901 gtttttattt tttacactgc caacgggcta acggctttat ttttcctcgc tcttacgcat
      961 tggagtgata ttcaagcaaa aatggaagat atatattatc catttttgtt atcggttata
     1021 tcggtggcgc cgattgtgat caacgctttt atcgttccgc gctttccaca aggtccgctt
     1081 tccaatgcag aagggatggc gttacgtcaa ttgccggtga tgttcgtagc actggtgctt
     1141 gtggcatgga attatcggtt tcttcatgtg gttcttttta gtatcgttac tacttttatc
     1201 gaacttgggc tggtcgcaat caacccatca gataatcgca acattaccgt atacattttc
     1261 atcgccatta ttcgtaccat tagttttgta gcggtgggtt tctttatcag tttcattgtg
     1321 tcacgtttgc gaaaacaacg tgagtcactt cgtgaagcaa atgccaatct cacgcactat
     1381 gcgtccacgt tggaacagct tacggtgagt cgcgagcgta accgtcttgc tcgtgaattg
     1441 catgacaccc tcgcacattc ccttactgcc atttccgttt cccttgaaac tgccaaagca
     1501 tatttcgata ttgacctgaa tcaatctcgt gaaatgatcg acacatcgct tgaagcgact
     1561 cgaaaaggtg tggatgaaac ccgccgcgcc ttgaaagcct tacgttcatc cgatttagtg
     1621 gatatgggct tgaggctcgc cgtgaaaaag gcggcagagt ccgccgcctc tcgcttctct
     1681 ctggacctcg aactggattt gcaagacccc atgccttctc tcagcccaga cgtagagcaa
     1741 accatcctac gtgtgacgca ggaagcgatt gagaacatga ccaaacattc acaagcgaaa
     1801 aaattcagtg tgcgtttgac tagcgataaa cacacgacat tagtcattca agatgatggc
     1861 attgggtttg atatgaaacc caaagactct acaggtcact ttggtttggt tggcatgcgc
     1921 gaacgtgctg agttggcggg tggcactcta aaaattgaaa gcggaaaggg aaaaggcacc
     1981 aaagtggtgc tgacgatcta attatttgac gatgtctatg gttttccatg gctatcgtcc
     2041 atcgtctacg atctatttat gaaaattcta ctttgtgatg atcaggcagt gatccgcgac
     2101 gggttggaaa tgcttcttca attggagaaa gatttccaag tgatcggttc tgctcaagac
     2161 ggatttgagg cagtagaact cgcttcaaaa aaatctccag accttgttct aatggacttg
     2221 aagatgccgg ggatgaacgg tattgaagct ac
//
LOCUS       DAOQNJ010000474         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_38937_length_2249_cov_9.930720, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000474 DAOQNJ010000000
VERSION     DAOQNJ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38937_length_2249_cov_9.930720"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLG52_11815"
     CDS             complement(<1..563)
                     /locus_tag="PLG52_11815"
                     /inference="COORDINATES: protein motif:HMM:NF015441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HNB42180.1"
                     /translation="MITVSSLTYYPIKACRGFDVPTSNIQRMGLEHDRRMMVITPDGE
                     FLTQREYPRLALVTPTLKNEAVTLSAPNVESIQFTVQSTGTPSPVNIWSSSGVDSIDQ
                     GDEAAAWFSNWLGISVRLVHMADGFKRKVNPIYAVNADDHTSFADGYPILIISEESLQ
                     DLNSRLDSALPMNRFRPNLVVKGCEPFA"
     gene            complement(560..1372)
                     /locus_tag="PLG52_11820"
     CDS             complement(560..1372)
                     /locus_tag="PLG52_11820"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNB42181.1"
                     /translation="MKSVENFIAEFKDKTISVALQDLETGMEFFSHADVMMHPASTMK
                     IPVMMEVFRQAEAGLFTLDDRLEIYNSFTSIADGSKFSLDVSDDSESTLYQRMGESET
                     IRELTRLMIVRSSNLASNLLMDKVSTARVDAIVKEYGIENMSIIRELEDKKALSLNMN
                     NAACARSSTQMLRLIAEGKIVSQKACDEMIRIFLGQEFNESIPAGLPNDVKVAHKTGW
                     SGEFFHDVGIVYPPNRKPYILSLFTHGFPENKDYEAHQCMAQISKLIYKEIL"
     gene            complement(1374..1661)
                     /locus_tag="PLG52_11825"
     CDS             complement(1374..1661)
                     /locus_tag="PLG52_11825"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNB42182.1"
                     /translation="MTAFVILDIEVTDPVGYEDYKKLAAPSLEPYGGKYAARGGKVEN
                     LEGDWSPTRIVVIQFESVERAKAWINSPEYSEARALRHKYAVSKAIVVEGL"
     gene            1810..>2249
                     /locus_tag="PLG52_11830"
     CDS             1810..>2249
                     /locus_tag="PLG52_11830"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HNB42183.1"
                     /translation="MTENEQLNPNEPIDDKYVLDKYNNKISYYWGASRSNKNSYKRYR
                     AWTVILGALVTLVSSISAAEFVQKPEWLRVAFAVATPVIAATLTVINGLGQNFHWGAT
                     WRDMVLNGTRLEKERDRFLATKPEDRDLVKELDRLNTIVLDETR"
BASE COUNT          593 a          513 c          538 g          605 t
ORIGIN      
        1 gcgaacggtt cacaaccttt caccacaagg ttcgggcgga agcgattcat cggcagcgcc
       61 gaatcaagcc gcgagtttaa gtcctgaagc gattcttccg agatgatcag aatcgggtag
      121 ccgtctgcaa agctggtgtg gtcatccgcg ttcactgcat agatcggatt cactttacgc
      181 ttgaacccat ctgccatgtg aacaagccgc accgagatgc ccagccaatt agagaaccat
      241 gccgcggctt catcgccctg atcgatggaa tccacaccgc tgcttgacca gatattgacg
      301 ggcgacggcg tgccggtaga ttgaaccgtg aattgaatgg attcaacatt aggtgcggat
      361 aaagtgactg cctcattctt aagggtgggc gtgaccagcg ccagacgcgg atattcacgc
      421 tgagtcagaa actcgccatc cggcgtgatg accatcatgc gccggtcatg ttcaagcccc
      481 atgcgttgaa tgttgctcgt gggcacatcg aacccacggc aggctttgat gggatagtaa
      541 gtgagtgagg aaacagtaat catagaatct ctttgtaaat aagttttgaa atttgagcca
      601 tacactgatg ggcttcgtaa tctttatttt ctgggaagcc atgtgtgaat aacgaaagga
      661 tataaggttt gcgattcggc gggtagacaa tgccaacatc gtgaaagaac tcgccagacc
      721 aacccgtttt atgcgcgacc tttacatcgt ttggcaaacc tgcaggaata ctttcgttga
      781 attcctgccc aaggaaaatg cgaatcattt catcgcaagc tttttgtgaa acgattttgc
      841 cttcagcgat caatcgtagc atctgcgtgg aactgcgcgc acaggcggca ttgttcatgt
      901 tcaggctcaa cgcttttttg tcttccagct cgcggatgat agacatattt tcgatgccat
      961 actcttttac aatggcgtcc actcgcgctg ttgagacctt gtccatgagt agattgctgg
     1021 cgaggttgga agaacgcacg atcatcaaac gggttagttc gcgaatcgtt tcgctctctc
     1081 ccatgcgctg ataaagtgtt gattctgaat catccgatac gtcaagcgaa aatttacttc
     1141 catcagcaat acttgtgaat gagttgtaaa tttctaagcg atcatcaagc gtaaacagcc
     1201 ctgcctctgc ctgacggaat acttccatca tcactggaat tttcatggta ctggcagggt
     1261 gcatcattac atcggcatga ctaaaaaact ccatgcctgt ttctagatct tgcaaagcaa
     1321 cgctgatggt tttgtctttg aattcggcaa taaaattttc gacagacttc aatttatagc
     1381 ccttccacca caatggcttt cgaaactgcg tatttatgcc gtaaagcacg cgcttcgctg
     1441 tattctggcg agttgatcca tgctttggcg cgttccacgc tttcaaattg gatcacaaca
     1501 atacgtgtgg gtgaccaatc gccctcaagg ttttcaactt ttccaccacg ggctgcatat
     1561 ttcccgccgt agggttcgag agaaggcgct gccagttttt tataatcttc gtatccaaca
     1621 gggtctgtta cttcaatgtc tagaattaca aatgcggtca tatctgactc cttagtgatt
     1681 tagcgatagg ttgtgtgacg caagattcta acattgactc ttgtgagggt aaaaaattcc
     1741 tgtgaaattt tgtaaaaaag tgtcataata gtggcaagaa aaaacgcatc ccgatgtgga
     1801 ggtatgataa tgactgaaaa tgagcaactc aacccaaatg aacctattga tgataaatat
     1861 gttttagata agtacaacaa caagatttcc tattattggg gcgccagccg tagtaataaa
     1921 aactcgtata aacgctaccg tgcttggaca gtgattctcg gtgcactcgt gaccttggtt
     1981 tcatcgatct cagccgcaga atttgtgcaa aagccggagt ggctgagagt cgctttcgcc
     2041 gtggcgactc cggtcattgc tgccacgttg acggtgatca atggattggg acagaacttc
     2101 cattggggtg ctacctggcg ggatatggtc ttaaatggca ctcgtcttga gaaagaacga
     2161 gaccgtttcc ttgcaaccaa gccggaagat agagacttgg taaaagaatt ggatcggtta
     2221 aacacaattg tgcttgatga aaccagaaa
//
LOCUS       DAOQNJ010000475         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_38973_length_2248_cov_13.467852, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000475 DAOQNJ010000000
VERSION     DAOQNJ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38973_length_2248_cov_13.467852"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PLG52_11835"
     CDS             <1..1221
                     /locus_tag="PLG52_11835"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNB42184.1"
                     /translation="AADVLTVLLLAKWGGCKTLPSITPLFEVIQDLKDAPIILEQLFT
                     SDYYRGHLQSHNHEQMVMIGYSDSNKDGGYVMANWSLYQAQEEITRVAAKHNVKLTIF
                     HGRGGTIARGGGPANNAIRAQPAGSINGKFRVTEQGEIIASRYANPGLAHRHLEQIAS
                     AVILASAPHKEEPIPAKWRETMETISNAGFRAYRSLVYETPGFIEFWEAATPLDEIKR
                     LQIGSRPASRAKAGAVNQIRAIPWVFSWMQSRFNLPSWYSLGSGLEAVADLSLLQEMY
                     DGWLFFRTLLNNSEISLLKADMDISALYVSLVPDQKLANDVFKTIRTEYERTCGAVLR
                     ISRHQNLLELEPLTQQAVQLRNPYVDPLNYIQVETLQRLRKLEDQDGDEAKSLREVMA
                     LTINGIAAGLRNTG"
     gene            1282..1854
                     /locus_tag="PLG52_11840"
     CDS             1282..1854
                     /locus_tag="PLG52_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB42185.1"
                     /translation="MVKIENSKRTILNLGEILLQDKGFNGFSYANIAAELGVKNAAIH
                     YHYPTKEDLGIAVMQRYRERFKLWINNARVKDLTPEGKLDWFLSIYTNMRADQGKICL
                     VGSMEAEFNSIPEGLQSEVQGLHKELLIWLQSTLAEGRDADAFAFKGEPADKAAMLLS
                     TLQGALQMARALGAKKFHAVIEQIKLDLLK"
     gene            1908..>2248
                     /locus_tag="PLG52_11845"
     CDS             1908..>2248
                     /locus_tag="PLG52_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42186.1"
                     /translation="MNALEPKVDSQAVIKKVYRAFFGPSKYEVKSSDEAILARGKNYR
                     IPFEGGELAVTTWGESGPSILLMHGWGGARAQMTGFVEPLLAAGYRVVAYDQPAHGES
                     DGKMTNVLEISP"
BASE COUNT          595 a          523 c          564 g          566 t
ORIGIN      
        1 gccgccgatg tgttgaccgt gcttttgctt gccaaatggg gcggctgtaa aacattaccg
       61 tctattacac cgcttttcga agtgattcaa gacctcaaag atgctccaat catccttgag
      121 cagcttttca cgtcggacta ttatcgcgga catttgcagt ctcataacca cgaacagatg
      181 gtaatgatcg gttattccga tagcaacaaa gatggtggtt atgtcatggc gaactggtct
      241 ctgtatcaag cgcaagaaga gattacccgt gttgctgcca aacataacgt caagctgacc
      301 atcttccacg ggcgcggtgg caccattgcc cgcggtggcg gacctgccaa caatgccatc
      361 cgtgctcagc ctgcggggag catcaatgga aaattccgtg ttacagagca aggcgagatc
      421 atcgcttcgc gctatgccaa ccctggtttg gcacatcgtc acttggagca gatcgccagc
      481 gcggtaatcc ttgcatctgc tcctcataag gaagaaccca ttcctgccaa atggcgcgaa
      541 accatggaga ccatttcgaa tgcggggttc cgtgcttatc gctcccttgt gtatgaaacc
      601 ccaggcttta tcgagttttg ggaagcagcg acgcctttgg atgaaatcaa gcgtttgcaa
      661 attgggtcac gcccggcatc tcgtgccaag gcaggtgccg tgaatcagat tcgagcgatt
      721 ccgtgggttt tctcatggat gcagagtaga ttcaacttac cgagctggta cagcctcggg
      781 tccggtttgg aggcggtggc tgatctttca ctccttcaag aaatgtatga tggctggcta
      841 ttcttccgca ccttgttgaa taattccgag atttcattac tcaaagcaga catggatatt
      901 tctgctttat acgtcagcct tgtgcccgat caaaaacttg caaacgatgt tttcaaaacc
      961 attcgcactg aatacgaacg gacgtgcggt gctgtattgc gaatcagccg ccatcaaaac
     1021 ttgctggaat tggagccgct cacgcaacaa gctgtgcaac ttcgcaaccc atatgtggac
     1081 ccgttgaact acattcaagt tgagactttg caacggctgc gaaagttaga agatcaggat
     1141 ggagacgaag cgaagtctct gcgcgaagtg atggcgttga ccatcaatgg catcgccgct
     1201 ggtctgagga ataccggata gtttgtgact ctaatgggga aggcttgaca acttactaac
     1261 tagtaagtaa aatttttacc aatggtcaag atcgagaact ccaaacgcac aattctgaac
     1321 ctgggcgaaa tcttattgca agacaaaggt ttcaatgggt ttagttatgc caacatcgct
     1381 gcagaattgg gtgtgaaaaa tgctgctatt cattatcact accctaccaa agaagatttg
     1441 gggattgcgg ttatgcagcg ttatcgcgag cgtttcaagt tatggatcaa taacgcccgc
     1501 gtcaaagacc tgacgccaga ggggaaactg gattggttct taagcattta cacaaacatg
     1561 cgtgcagacc aaggcaagat ttgccttgtc ggttccatgg aagcagaatt caattccatt
     1621 cccgaagggt tacaaagcga agttcaaggc ttacataaag aattgctaat atggcttcag
     1681 tccactttgg ctgaaggcag agatgctgac gcctttgcct tcaaaggaga gcccgcagat
     1741 aaagccgcaa tgcttttatc gaccctgcaa ggcgcattac aaatggcgcg tgccttgggc
     1801 gctaagaaat tccacgctgt gatcgaacag attaagttag atttactcaa ataaattttt
     1861 tttgtcaata aaacttacta ggtagtaagt tttcaaaagg agaaactatg aatgctttag
     1921 aaccaaaagt tgattcgcaa gctgtaataa agaaggtgta tcgcgccttt ttcggaccga
     1981 gcaaatatga ggtgaaatca tcggatgaag ctattcttgc tcgaggaaag aattatcgca
     2041 tccccttcga gggtggcgag ctggctgtca ccacatgggg cgaaagcgga ccatccatct
     2101 tattaatgca tggctggggt ggagcacgtg cccaaatgac cggtttcgtt gagccgcttc
     2161 ttgcggcagg gtatcgagtg gttgcatacg accaacccgc tcacggcgag tcggatggca
     2221 agatgaccaa tgtgcttgag atttcccc
//
LOCUS       DAOQNJ010000476         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_39162_length_2242_cov_6.748514, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000476 DAOQNJ010000000
VERSION     DAOQNJ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39162_length_2242_cov_6.748514"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1009
                     /locus_tag="PLG52_11850"
     CDS             29..1009
                     /locus_tag="PLG52_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB42187.1"
                     /translation="MDNVLNWGLLSTAKINRALIKPLRASKRTRLLGVASRSQSSADE
                     YAREWKIPRAYGSYESMLADPEIDIVYNSLPNHLHEEWTVKALRAGKHVLCEKPLALT
                     VEEVDRIIAASNETGKVAAEAFMYRHHAQTLKIKELVDSNLIGQIQLIKGAFTFTLNR
                     EGNYRFIKEYGGGSLWDVGCYPISFARMVVGEEPVEVFGSQVLGPGGSDDSFYGQMRF
                     GNGIHMQFDSGFKSPFRSYMEIVGTAGVINIPAPFKPGRKSEIYITRGETQEKIKISA
                     NELYADEVEDMCNAVLNGKTPRVSLLDSRGNIATILAFLQSATEGKLVQI"
     gene            1117..2217
                     /gene="ugpC"
                     /locus_tag="PLG52_11855"
     CDS             1117..2217
                     /gene="ugpC"
                     /locus_tag="PLG52_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNB42188.1"
                     /translation="MASVTYNNVIKSFGDVNVIKQLNFKVEDKEFLVLVGPSGCGKTT
                     ALRMLAGLEEISAGEIMIGDRVVNDVAPKDRDIAMVFQSYALYPHMSVYDNMAFGLKL
                     RKTPKDEIKKRVSEAAEILDITHLLDRKPRQLSGGQRQRVAVGRAIVREPKVFLFDEP
                     LSNLDAKLRVQMRAEISKLHQRLQTTFIYVTHDQTEAMTMATRIAVINKGLLQQLDTP
                     QVLYDRPANLFVAGFIGSPSMNFFPGKISVSGGKVTVDMDTFKVSIPDALAGPYKNMD
                     GKKIVFGIRPENIHDPEFAPVNIHGEKVAAKVDVTELMGNETFLYLVNGKNTFIARVD
                     PRSHKRVGDDVQVIFDMDKFHIFDVDTEEAIR"
BASE COUNT          605 a          548 c          533 g          556 t
ORIGIN      
        1 ttcattctca accaaaggag taacacaaat ggacaacgtt cttaattggg gattgctctc
       61 caccgccaaa atcaaccgcg ctttgattaa acccctgcgc gcctccaaac ggacccgctt
      121 actgggtgtc gcttcgagaa gccaatcctc cgctgacgaa tatgcccgcg aatggaaaat
      181 ccctcgtgct tatggcagtt atgagtccat gctcgctgac cctgagatcg atattgtcta
      241 caactctttg cccaatcacc tgcacgaaga gtggacagta aaagctttgc gagcagggaa
      301 acatgtttta tgcgaaaaac cactggctct tactgtagaa gaagtagacc gcatcattgc
      361 tgcctcgaac gaaacaggca aggtcgccgc cgaagcgttc atgtatcgtc atcatgcaca
      421 gacactaaaa ataaaagaat tggtagatag caacttgatt ggacaaattc agcttatcaa
      481 aggcgctttt acgtttactc tcaatcgtga gggaaattat cgcttcatca aagaatacgg
      541 cggtggcagt ttatgggatg tgggctgtta cccgatctcg ttcgcgcgaa tggttgtcgg
      601 cgaggaaccg gttgaggtat ttggctcaca ggttctcggt ccgggaggaa gcgacgactc
      661 gttctacggg cagatgcgtt tcggaaatgg aatccatatg cagtttgaca gcggattcaa
      721 atctccattt cgaagttaca tggaaattgt tggcacagca ggtgtcatca acattcctgc
      781 cccgtttaag cccggaagaa aaagtgaaat ctacatcacg cgcggtgaaa cacaagagaa
      841 aataaaaatc tctgcaaatg aactttacgc agacgaggta gaagatatgt gcaatgcggt
      901 tttgaatggg aaaacacccc gcgtttctct tttagacagc cggggcaaca ttgccacaat
      961 tctggctttt ctccaatcgg ctaccgaggg gaaactggtc caaatttagg gactttgttg
     1021 actctcgcaa ctaaatcccg ccccaaaatt ataggtcaaa aatgcccatg tgattataat
     1081 aatgtcacta tactcgttcg gactggaggc aataccatgg caagtgttac atacaataac
     1141 gtaataaaga gcttcggtga cgtcaatgtc atcaagcaac tcaacttcaa agttgaggat
     1201 aaggaatttc tcgttctcgt tggaccttcc ggctgcggta agacaacagc tcttcgcatg
     1261 ctggcaggat tggaagagat cagcgctggt gaaattatga tcggtgatcg cgtggtgaac
     1321 gatgtcgctc caaaagatcg cgacattgcc atggtattcc aatcctatgc gttgtatccg
     1381 cacatgtcgg tgtacgacaa tatggcattc ggtttgaagc tacgcaagac accgaaagat
     1441 gaaatcaaga agcgcgtcag cgaagctgcc gaaattctcg atatcactca cctgctggac
     1501 cgcaagcccc gtcagctttc tggtggtcaa cgccagcgtg ttgcggtagg tcgtgccatc
     1561 gttcgcgaac cgaaggtctt cctcttcgat gagccgctct ccaacctcga tgccaagctt
     1621 cgtgtgcaga tgcgcgccga gatcagcaaa ctccaccaac gcttgcaaac caccttcatc
     1681 tatgtgaccc acgatcagac agaagccatg accatggcaa ctcgcatcgc ggtgatcaac
     1741 aaaggtttgc tccaacagtt ggatactcct caggttctgt atgatcgccc tgccaatctc
     1801 tttgtggctg gcttcatcgg ctctccttcc atgaacttct tccctggcaa gatctccgtc
     1861 agtggcggca aagtgaccgt ggatatggat accttcaagg tcagtattcc ggatgcgttg
     1921 gctggtccat ataagaatat ggatggcaag aaaatcgttt tcggtattcg tcctgaaaac
     1981 attcatgatc ctgaattcgc tcccgtcaac attcacggtg aaaaagttgc cgccaaagtg
     2041 gatgtcactg aattgatggg taatgaaacc ttcctctatt tagtcaatgg caagaatact
     2101 ttcatcgccc gcgtcgatcc tcgttcgcat aaacgcgttg gtgatgatgt gcaagttatc
     2161 ttcgacatgg acaagttcca catcttcgat gtggatacag aagaagccat acgataataa
     2221 cgtgaaagga ggtggtcccc tc
//
LOCUS       DAOQNJ010000477         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_39543_length_2231_cov_7.462316, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000477 DAOQNJ010000000
VERSION     DAOQNJ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39543_length_2231_cov_7.462316"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLG52_11860"
     CDS             complement(<1..606)
                     /locus_tag="PLG52_11860"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNB42189.1"
                     /translation="MSKARKLIPYFIVFDILVLIAVIVALNFDVVLRLNNAYKAGTLT
                     EQKIRYEIAAYDLNDWRRVEPIDQKTSPHDGMVQVYVPEGEFIMGKPGEPDFDSPEHL
                     VYVNSFWIDRVEISNQLYAACVEAGGCSQPFIDENPFYGRWVYRNLPVTFVNWYQATE
                     YCEWAGRRLPTEAEWEKAARGTDQRPYPWGEADATPRLANYA"
     gene            complement(636..>2231)
                     /locus_tag="PLG52_11865"
     CDS             complement(636..>2231)
                     /locus_tag="PLG52_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNB42190.1"
                     /translation="TPWYHKTSIYHIYPRSFMDSNGDGIGDIQGIIQKLDYIRDLGFD
                     SIWSSPFFSSPQADFGYDISDYQNISPEFGTLDDAFQLIEEVHKRGMHIIFDLVLNHT
                     SNEHPWFKESRSSKNNPKSDWYIWRDKPNNWNSMTGGNGWHYAKERGQYYWCSFLSFQ
                     PDLNWRNSEVKQTMFNIARFWLEKGVDGFRLDIFNTIYKDAEFRNNPFTFKLAPTPDD
                     PSGYFQKAQYSQDQPESFELAKELRQVTNEFGERLTVGEITAKRNIVRKFAGEEKNDG
                     LTLAFDFEMLEFTFTAEYFRKLIGNIEMHFHPPFMPVYVFSNLDKRRSIHRLGDDVRK
                     AKLLHMLQLTVRGVPCIYYGEEIGMSNLKLPFNTALDPIPHKYTFAPRFIFDILDITV
                     NRDEVRTPMQWDTTKNAGFSSAEKTWLPVHPNHSLVNVESESKDEHSLLNTIKSLMKF
                     RNENSAIKEGDFEFIDGLPAGVLGYKRSLNGQSFLVLLNFDETAKDVRLRFQNAAFRL
                     TVKDEAQGDIAHLNSFGGLILFV"
BASE COUNT          584 a          505 c          527 g          615 t
ORIGIN      
        1 ggcgtagttc gcgaggcgcg gagttgcatc cgcttcgccc cacggatatg ggcgttgatc
       61 ggttccacgt gcagcttttt cccattctgc ttcagtaggc aaacggcgtc cagcccattc
      121 acagtattca gtagcttgat accagtttac aaaagttact ggcaaattgc gatagaccca
      181 tctgccataa aatggatttt catcaataaa cggttgcgaa caaccaccag cttccacaca
      241 agcagcatac agttggttag atatttctac gcggtcaatc caaaatgaat tcacatacac
      301 gagatgctca ggcgaatcaa aatctggttc gccgggcttg cccataataa attcaccttc
      361 aggtacatac acttgcacca taccgtcatg aggcgaggtc ttttggtcaa taggctcaac
      421 acgccgccaa tcgttcaagt catatgcagc aatctcgtag cggatctttt gttctgtcag
      481 cgtgccggct ttgtatgcgt tattcagacg cagcactaca tcaaaattga gcgcaacaat
      541 tactgcaatc aacacaagga tatcaaaaac aatgaagtaa ggtatcagtt ttcgggcttt
      601 agacatgggc gacattatac ctagcacaga tgcctttata caaaaagaat cagcccgccg
      661 aaactgttca agtgcgcaat gtcgccctgc gcttcatcct taactgtgag cctgaatgcg
      721 gcattctgga accttaagcg cacatccttt gctgtctcgt cgaagttcaa gagcacgagg
      781 aaagattgtc cgttcagaga acgtttgtat ccgagtacgc ctgctggcaa cccgtcaatg
      841 aattcaaaat ctccttcttt gatggctgaa ttctcattcc tgaatttcat caaagatttg
      901 atggtgttca ataaggagtg ttcatcttta ctttcacttt ccacattcac aaggctgtga
      961 tttgggtgaa caggcagcca cgttttttca gcggacgaaa accctgcgtt ctttgtggta
     1021 tcccattgca tgggtgtacg gacctcgtcg cggttcacgg taatatcgag tatgtcgaag
     1081 ataaagcgtg gggcaaaagt gtatttatgc ggaataggat caagcgccgt gttgaatggt
     1141 aatttcaaat tgctcatacc gatctcttcg ccataatata tgcaaggcac accgcgcaca
     1201 gttagttgca acatgtgaag cagcttcgcc ttgcgcacgt catcgccgag gcggtgaatg
     1261 ctacggcgtt tgtcgagatt gctaaataca tagaccggca taaaaggcgg atgaaaatgc
     1321 atttcaatat tgccaattaa cttgcggaag tattcggctg taaatgtgaa ttcgagcatc
     1381 tcaaaatcga aggcaagggt cagcccatca ttcttttctt ctcccgcaaa cttgcgcacg
     1441 atattccgtt ttgccgtaat ttcaccgacc gtgaggcgtt caccaaattc gttggtaact
     1501 tgtctcagtt cctttgcaag ttcaaagctt tcaggttgat cttgtgagta ttgtgctttc
     1561 tgaaaatacc cagaggggtc atcgggggtg ggggcaagtt tgaatgtgaa aggattattt
     1621 cgaaactcgg catctttgta aatcgtgttg aaaatatcca aacggaagcc atccacacct
     1681 ttttcgagcc aaaaccgggc aatgttgaac atggtttgct tgacttcgga attgcgccag
     1741 tttaaatcag gttgaaagga caggaaactg caccagtagt attgtccgcg ttctttggcg
     1801 tagtgccagc cattcccgcc ggtcatactg ttccagttgt tgggtttgtc acgccagatg
     1861 taccaatctg attttgggtt gttttttgaa gaacgtgact ccttaaacca tggatgttca
     1921 ttggaggtat ggttcagtac aaggtcaaag atgatgtgca ttccacgctt gtgaacttct
     1981 tcgatgagtt gaaaggcatc atccagcgtg ccaaattcag gcgagatgtt ctggtaatcg
     2041 gagatgtcgt atccaaaatc cgcttgaggc gaggagaaga agggtgaact ccagatggag
     2101 tcaaagccca agtcacgaat gtagtccagc ttttgaataa tgccttgaat gtcgccaatg
     2161 ccgtcgccat tgctgtccat gaacgagcgc ggatagatgt gatagatact tgttttgtga
     2221 taccagggtg t
//
LOCUS       DAOQNJ010000478         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_39935_length_2219_cov_11.225508, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000478 DAOQNJ010000000
VERSION     DAOQNJ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39935_length_2219_cov_11.225508"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PLG52_11870"
     CDS             complement(<1..971)
                     /locus_tag="PLG52_11870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB42191.1"
                     /translation="MKIALLSEKYTPDLGGLAISTGRLGEMLASAGHDVRLFAPTTSL
                     PPTIKQTQRSGGVLVTRFGAHKRVDDTLVDWFELIVEEHKREPFDLIHAYFLPMAGFV
                     GTYAGKYLGIPSVVSIRGNDIERAAFDPSKFSHVMYALQNANAVTTNATVLAKKTIAF
                     VEREIHVIPNGVDTGRFKPMERNEVLAEAIGLSMESGGLPPEIGKQAPRLHKYVIGFV
                     GELREKKGMATLLAGYAQIAKTIPASLLIVGEVREGEDKKVFEEFKISNPHLPITVTG
                     HVAHKDLPAYYSLMDVFVHPSLRDGMPNAMLEAMACGKTVIATPVGGV"
     gene            983..1261
                     /locus_tag="PLG52_11875"
     CDS             983..1261
                     /locus_tag="PLG52_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42192.1"
                     /translation="MAKCEMVIGYLVPHRCDNPALGTCVKCGRGFCDEHTNATNEGRV
                     CLACQQGLDMPVALPIAASTFTAADLATFGHASTWDDDDSNDMFSDLS"
     gene            1268..1885
                     /locus_tag="PLG52_11880"
     CDS             1268..1885
                     /locus_tag="PLG52_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB42193.1"
                     /translation="MNVLLLHGQGRTTNAMRLLGFRLHRLGYHVCYFRYYTRIEKFSG
                     IVERLTDTIKKLPNDRPYALVGHSMGGLLARACLPSLKDHPPFHLILLASPNRPPQLA
                     PYIKHNRIYKYLTNDCGQQALNAEFYNTLPKPFVPTTIIAGSGGPRLHWLPYGDQPND
                     GVMSVQDTRLGPEYGVIQVPSIHTFIMNSRQTLSHIDRILRQTGI"
     gene            complement(1882..>2219)
                     /locus_tag="PLG52_11885"
     CDS             complement(1882..>2219)
                     /locus_tag="PLG52_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNB42194.1"
                     /translation="EDAFPFGRDESEVRLGRAGRYAFGFYEKHPCYAPWSHTLIDFNG
                     NVYVCCMTREKIPPLGNIIHQSFKEIWEGAAYRRVRLKMHPPSLKPCARCDDFIDENK
                     KIWEMIGPY"
BASE COUNT          523 a          548 c          561 g          587 t
ORIGIN      
        1 acaccgccaa cgggagtcgc aatgaccgtc tttccgcacg ccatcgcttc gagcatcgca
       61 ttcggcatgc catctctcaa agacggatgc acgaaaacat ccatcaaaga ataatatgcg
      121 ggcaagtctt tgtgggcgac atgtccggta accgtaatag gtaagtgggg attggagatt
      181 ttgaattctt caaatacttt tttatcttca ccctcgcgga cctcaccaac aatgagcagg
      241 gaggcgggga ttgtttttgc tatttgcgcg tagccagcta gtagggtagc cattcccttc
      301 ttttcacgca attcacccac aaagccaatt acatatttat ggagtcgggg agcttgcttc
      361 ccgatttccg gcggtaagcc gccagactcc atagatagcc caatcgcctc tgccagtacc
      421 tcattcctct ccatcggctt aaagcgtcca gtatcaaccc cattcgggat gacgtgaatt
      481 tcacgttcaa cgaatgctat tgtttttttc gctaatactg tggcattggt cgtcacagca
      541 ttggcgtttt gcaaggcata catcacatgc gagaattttg aggggtcgaa ggcggcgcgt
      601 tcgatgtcgt ttccgcgaat ggagacaacg ctgggaatgc ctaaatattt tccggcatag
      661 gtgccaacaa atccagccat tggcaaaaag taggcatgta tgagatcgaa cggctcacgc
      721 ttgtgttcct ccacgatcaa ctcgaaccaa tcgaccagag tatcgtctac gcgcttgtgt
      781 gcgccaaagc gcgttacgag gactccgcct gagcgttggg tctgtttgat ggtgggtggc
      841 aggcttgtag tcggggcaaa gaggcggacg tcatgtccag ccgaggcaag catctccccc
      901 aatcgtcctg tagagattgc gagccctcca aggtctggtg tatacttttc gctaagcaaa
      961 gcaattttca tgaggtgtct caatggcaaa gtgtgaaatg gttatcggtt atctggttcc
     1021 gcatcgttgt gataacccgg cgttggggac ctgcgtcaaa tgcgggcgcg ggttttgtga
     1081 tgaacatacg aatgcaacca atgaaggacg agtttgtctt gcctgtcagc agggtttgga
     1141 tatgccggtt gctttgccga tcgctgcttc aacctttact gctgccgatc tggctacttt
     1201 tggtcatgcc agtacgtggg acgatgatga cagcaacgat atgttttcgg atttgagttg
     1261 aaaatccatg aacgttcttt tgcttcatgg tcaggggcga acaacgaatg ccatgaggct
     1321 gcttggtttc cgtttgcaca ggctcgggta tcatgtgtgc tatttccgat attacacccg
     1381 cattgaaaaa ttcagcggga tcgtggagcg gttgaccgat acgatcaaaa aactaccgaa
     1441 cgatcgacca tatgccttgg tggggcattc catgggtggg ttgctggcac gagcctgcct
     1501 tccttcattg aaggatcatc cgccatttca tttgatttta ctggcttctc caaaccggcc
     1561 tcctcaattg gcgccttata taaaacataa ccgtatctat aaatatctga ccaatgactg
     1621 tggacaacag gcgttgaatg cagagtttta taacacgctg ccaaagccat tcgttccgac
     1681 gacgatcatt gctggctctg gcggccctcg gctgcattgg ctgccttatg gtgaccagcc
     1741 aaacgacgga gtcatgagcg ttcaggatac tcgtcttgga ccggaatatg gcgtgatcca
     1801 ggttccaagt attcatacgt ttatcatgaa ttcacgtcag accctttccc atatcgatcg
     1861 cattctaagg cagacgggga tttaatacgg tccgatcatt tcccagatct ttttattctc
     1921 atcaataaaa tcgtcacaac gtgcgcatgg cttgagggaa ggcggatgca ttttcaatcg
     1981 tacccgacga taggccgcac cttcccaaat ttccttgaat gattggtgga tgatgtttcc
     2041 caagggtggg attttttcac gcgtcatgca acagacatag acgttgccat tgaagtcgat
     2101 caatgtatgc gaccagggag catagcaggg atgcttttca tagaagccga aggcgtatct
     2161 ccccgcgcgt cccaagcgga cctcggactc gtctcgccca aagggaaaag catcttcat
//
LOCUS       DAOQNJ010000479         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_39973_length_2218_cov_9.811373, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000479 DAOQNJ010000000
VERSION     DAOQNJ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39973_length_2218_cov_9.811373"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>2218
                     /locus_tag="PLG52_11890"
     CDS             36..>2218
                     /locus_tag="PLG52_11890"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42195.1"
                     /translation="MLKNFVKLFSGDPTKKTVDQYRPLVEQVNALEAQFTSLSDDALR
                     AKTDEFKKRIVELTGNVEGLEEKERIKAEQNALEQIMPEAFAAVREASKRTIGLRHYD
                     VQIIGGAALHSGSIAEMRTGEGKTLVATMPVYLNSLLGRGVHLITVNDYLARRDARWM
                     APIYHALGLTVGVLQMAAVTENGKKAFIVDFERDAPQEDQRHMRLVERVEAYRADVTF
                     GTNSEFGFDYLRDNMAMRLENRVQRGHYFAMVDEVDNVLIDEARTPLIISGPASGDLE
                     WYGRMAQIVKQLKPEDYEMEEKSRTINLTEIGVSRVESMLGVQLLDPDRPEDLNPEQA
                     RLMGYLQQSLRAQFLFTRNKDYLVQGGKVVIIDESTGRLMPGRRWSDGLHQAVEAKEG
                     VKVEPESVTYATVTLQNYYRMYQKLAGMTGTALTEAEEFNKIYKLEVAPISPNLEYLS
                     YGKEATLTEIKTKDEEGYAYSYFAKVGDTKPLFFRRKDYPDVIYRTVEAKLRAIVQEI
                     VRYNALGRPLLVGTTSVESSERLSNRLKAEPVRRLMQYFLVRDHYIRANNIEEDGRLI
                     ADLVPFNEPIEKITPDALRAFIKPHGLTNISLDDDTNLQTLLDILSLPSESKDRLKKI
                     FQGGVLHQVLNARRHTEESQIIAGAGAFGAVTIATNMAGRGVDIKLGGELAEEVISAV
                     NRVLGKTGLKDPFDMTHDERRVELKKLDATAYGLYEAEVKNFLQY"
BASE COUNT          564 a          591 c          574 g          489 t
ORIGIN      
        1 ggaagggact ttccctctcc attgaaaggg atttcatgct taagaacttc gtaaaactat
       61 tcagtggtga ccccacaaaa aagaccgtag accaataccg cccgcttgta gagcaggtca
      121 atgctttgga ggcgcagttt acgtccctga gtgacgatgc gttgcgtgcc aagacggatg
      181 aatttaagaa gcggatcgtt gaacttactg ggaatgtaga aggtctggaa gagaaagaac
      241 gcatcaaagc cgaacaaaac gcgctcgaac aaatcatgcc cgaagccttt gcggcagtcc
      301 gtgaagcctc gaagcgcacc attgggcttc gtcactacga cgtgcagatc atcggcggtg
      361 cggcgttgca cagcggctcc attgcagaga tgcgaaccgg cgaaggcaag accctcgtgg
      421 ctaccatgcc ggtctatctc aattccctgc tcggacgagg cgttcacctc attacagtca
      481 atgattatct ggcacgacgc gatgcccgtt ggatggcgcc tatctatcat gccctcggct
      541 tgaccgtggg tgtgctgcaa atggcggctg tcaccgagaa tggcaagaaa gcattcattg
      601 tggattttga acgggacgcg ccccaagaag accagcgtca catgcgcctc gtggagcggg
      661 tcgaagccta tcgcgccgac gtgaccttcg gtaccaactc tgagtttggt ttcgattatc
      721 tgcgcgacaa catggcaatg cgacttgaga accgcgtcca acgtggacat tacttcgcca
      781 tggtcgatga agtcgataac gtcctcatcg acgaagcgcg taccccgctc atcatttccg
      841 gtcccgcctc aggtgacttg gaatggtacg ggcgcatggc gcagatcgtt aaacagctca
      901 agcccgaaga ctatgagatg gaagaaaaat ctcgcaccat caacttgacc gagatcggtg
      961 tgagccgggt tgagtctatg ttgggtgtac aactgctcga ccccgatcgc cctgaagacc
     1021 tcaaccccga acaagcacgc ctcatggggt atttgcaaca atccctgcgt gctcagttcc
     1081 ttttcactcg caacaaagat tatttggtgc agggcggtaa agtcgtcatc atcgacgagt
     1141 caacggggcg tctcatgcct gggcgtcgtt ggagcgatgg tttgcatcaa gctgtggaag
     1201 ccaaagaagg cgtaaaggtc gagcccgagt cggtcaccta tgccacggtc actttgcaga
     1261 actactatcg tatgtatcaa aagttggcag gtatgaccgg taccgcgctg accgaagctg
     1321 aagagtttaa taaaatctac aagctcgaag tcgcgccgat ctcgccgaac cttgaatatc
     1381 tttcctacgg caaagaagcc acactcactg agatcaaaac aaaagatgaa gaagggtatg
     1441 cgtattccta ctttgccaaa gtgggcgata ccaaaccgct tttcttccgc cgcaaagatt
     1501 atcccgatgt catctatcgc acggtcgaag ccaagttgcg cgccattgtg caagagatcg
     1561 ttcgctacaa cgcattagga cgcccactgt tggtgggtac cacttccgtt gaatcttctg
     1621 aacgtctctc gaatcggctc aaagcagaac cggttcgacg tttgatgcaa tacttcctcg
     1681 tgcgtgacca ttacatccgc gccaataaca ttgaggaaga cgggcgcctc attgcggacc
     1741 tcgtgccttt taacgaaccc attgagaaaa tcacccccga cgccttacgc gccttcatca
     1801 aaccccacgg tctcaccaac atcagcctcg acgatgacac caacctgcaa accttgctcg
     1861 atatcttgag cttgccaagc gaatccaaag atcgtttgaa gaaaatcttc caaggcggag
     1921 tgttacatca ggtcttgaat gcccgccgcc atactgaaga gtcgcagatc attgcgggcg
     1981 cgggtgcgtt cggcgctgtg accattgcta ccaacatggc aggtcgtggt gtagacatca
     2041 aactcggcgg cgagttggca gaagaagtta tttcggcagt caaccgtgtg ctaggtaaaa
     2101 ccgggttgaa agacccattt gatatgaccc acgatgaacg ccgcgtggaa ttaaagaaac
     2161 ttgatgccac cgcttatggt ttgtatgaag cagaggtcaa gaacttcctg caatactt
//
LOCUS       DAOQNJ010000480         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_40133_length_2213_cov_8.995366, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000480 DAOQNJ010000000
VERSION     DAOQNJ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40133_length_2213_cov_8.995366"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PLG52_11895"
     CDS             complement(<1..748)
                     /locus_tag="PLG52_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNB42196.1"
                     /translation="MEFTEKRQHWKKINALTPKKMELAHFLLNKIREGEDVMTLIRRH
                     PLEGGGYLNKAALVAAYEQMVASGEMEVDTALLERIRMKPMRTLSGVTTVTVLTKPYP
                     CPGKCIFCPTDVRMPKSYLPDEPGAMRALEHKFDPYEQVQNRIRALKNLGHPTDKIEL
                     LILGGTWSSYRRDYQEWFIGRCFDAMNSTEVLSPTEPERTEQMDSAVLTNIHSVNETA
                     PHRNVGLVIETRPDEINPDEIKWLRHLGVTK"
     gene            complement(774..1517)
                     /locus_tag="PLG52_11900"
     CDS             complement(774..1517)
                     /locus_tag="PLG52_11900"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNB42197.1"
                     /translation="MLSAQDIEKLSLEFETKTPQEIIRWAIKEYAPNIAMSSSFQTQS
                     IPLLHMVSQIDHTVTVFFIDTGYHFWETLMFREQIAMEWKLNVFDLYRDTRWDMFALQ
                     NVRKLPVEDPNLCCYLNKVQPMQKALQGVKAWISGIRRDQTEVRAHANILELQDDGLL
                     KVNPLLNWKKADVENYMKEHALPSHPLLEKGYRSVGCAPCTIAIGVNDDERAGRWAGR
                     GKTECGLHTEMFKKKKLTDVQQEFQMEIK"
     gene            1556..>2213
                     /locus_tag="PLG52_11905"
     CDS             1556..>2213
                     /locus_tag="PLG52_11905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB42198.1"
                     /translation="MDANITKQLIELNRKFYDQFGESFSATRQRLQPGVKKILETIHA
                     DESVLDLGCGNGFFLRELHDRGHYAPLLGADFSLPLLREAESTPGVEFRELDLMQLSA
                     FSNQLSVAGGWDVITMFATMHHIPSREVRLDILHTVKKLLKPNGKFILSNWQFLNSEK
                     LKARVQPWGKAGLSDSDVDEGDYLLDWRSGGEGLRYAHQFSEQELLGLAEQARMKVEA
                     S"
BASE COUNT          517 a          539 c          560 g          597 t
ORIGIN      
        1 ctttggtcac accgaggtga cgcagccact tgatctcgtc gggattgatc tcatccggtc
       61 gggtttcgat gaccaagccc acgttgcggt gaggcgcggt ttcattcacc gaatggatat
      121 tggtcaatac tgctgaatcc atttgctcgg ttcgttctgg ttcggtgggc gaaaggactt
      181 cagtggaatt catcgcatca aaacagcgac cgatgaacca ttcctgataa tcgcgtcgat
      241 acgaagacca tgtaccgccg aggataagca attcgatctt atctgtggga tgaccgagat
      301 tctttaaagc acgaatgcgg ttttgcacct gttcgtacgg gtcaaattta tgttcaagcg
      361 cgcgcatcgc gccgggttcg tcgggcagat agcttttggg catgcgcaca tcggtagggc
      421 agaagatgca tttgccgggg cagggatagg gcttggtcag caccgtcacc gtggtcacgc
      481 cagagagggt gcgcatcggc ttcatacgga tgcgttccag caaagctgtg tccacttcca
      541 tttcgccgct ggcaaccatc tgctcatagg cagccaccaa cgccgccttg ttcaaatatc
      601 ctccaccttc gagcgggtga cgacgaatga gcgtcatcac atcttcgccc tcgcggattt
      661 tattaagcag aaaatgcgcc aactccattt tctttggggt cagcgcgttg attttcttcc
      721 agtgttgtcg tttttcggta aattccatca acagcctata aagtgaatgc cccttatttt
      781 atctccattt gaaactcttg ttgcacatcg gtcaactttt tctttttgaa catctcggta
      841 tgcaagccgc attccgtctt tccgcgccct gcccagcgac ccgcgcgttc atcgtcattg
      901 acgccgatgg caatcgtgca aggggcacag cctacactgc gatagccttt ctcaagcaat
      961 ggatgagacg gcaacgcatg ttctttcatg tagttttcca cgtctgcttt tttccagttg
     1021 agcagcgggt tcacttttag caagccgtca tcttgtaatt ccaaaatatt ggcatgcgcg
     1081 cgcacttcag tttggtcgcg gcgaatgcca gatatccacg ctttcacgcc ttgtaacgct
     1141 ttttgcatgg gttgcacttt gttgagatag caacaaagat tggggtcttc cactggcaac
     1201 ttgcggacgt tttgcagcgc aaacatatcc cagcgagtat cgcggtatag gtcaaacacg
     1261 ttgagcttcc attccatcgc aatctgttcg cggaacatca gcgtttccca aaaatggtag
     1321 ccagtatcaa taaagaacac agtcaccgtg tgatcgatct gcgacaccat atgtaacaac
     1381 ggtatcgact gtgtttgaaa cgacgagctc atggcgatat ttggcgcgta ttctttaatc
     1441 gcccaacgga tgatctcttg cggggttttg gtttcaaatt ctaaagagag tttttcaata
     1501 tcttgtgcgg aaagcatgaa aaaattatac cgtcctaaac cgttataatt tccctatgga
     1561 tgccaacatt acaaaacagt taattgaact gaaccgcaaa ttttatgacc agtttggcga
     1621 gtcattttct gcaacacgtc agcgcttgca gccgggggtg aagaagattc ttgaaacgat
     1681 ccatgcggat gaatctgtgt tggacttggg ttgtggcaac ggatttttcc tgcgtgaact
     1741 gcatgatcgc ggtcactatg ccccgcttct tggcgcggac tttagcctgc ctctgctacg
     1801 agaagccgaa tccacgccgg gagttgagtt tcgagagctg gatctcatgc agctttcagc
     1861 cttcagcaat cagctttcag tggcaggcgg ctgggatgtg atcacgatgt ttgcaactat
     1921 gcatcacatc ccttcgcgag aagtccgctt ggatattttg cacacggtga aaaaattact
     1981 caagccaaat ggaaaattca tcctttcgaa ttggcaattc ttgaacagcg aaaagttaaa
     2041 agccagagtc caaccgtggg ggaaggcagg gctttcagat tcagatgtgg atgaagggga
     2101 ttatctgctc gattggcgca gtggtggtga agggttgcgg tacgcgcatc aattctcaga
     2161 gcaggaactg cttgggttgg cagagcaagc ccgaatgaag gttgaagcgt ctt
//
LOCUS       DAOQNJ010000481         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_40195_length_2211_cov_9.479592, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000481 DAOQNJ010000000
VERSION     DAOQNJ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40195_length_2211_cov_9.479592"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1159
                     /locus_tag="PLG52_11910"
     CDS             386..1159
                     /locus_tag="PLG52_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNB42199.1"
                     /translation="MKLYKPTLVLVLTTLLVACAPATEEASHASTPHWTYEGEEGPEH
                     WGDLDPSYAACETGTKQSPIDVSNPAEQDLANIAFHYQPSEVNILNNGHTVQVNYDAG
                     SYIELDGVRYDVAQFHYHAPSEHAVDGKLFAAELHIVHKNADGGLAVVGILLDEGAEN
                     AAYQPFIQNLPAEESDVKDAGVTVNAADLLPAVQTTFRYSGSLTTPPCTEGVNWLLMT
                     TPVEISASQIEALASLFEAGNNRPVQPLNDRPLAEDSTP"
     gene            1234..2160
                     /locus_tag="PLG52_11915"
     CDS             1234..2160
                     /locus_tag="PLG52_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42200.1"
                     /translation="MPLRFPVTSLVLIWMLVGCSSLPFVATPTPTALPTSTPTRTPLP
                     TQTPSPTVTPILVTYPFKHVWFKYTVERPAGEANPLGLTEEPLLVIYTDGTMIAARTG
                     DPKVRTRVLNTKEVCDFLNRLDTFGFYMLEDSHSTDETNPLYDFGTKYQAVDEGDFAS
                     LTVNRTDPKSVTFFEPYRKFFARPMRKIMEFVETYNPGALKAYEPDRLVVFVQRGRAE
                     GTDEKVRVRDWTVNTFSLNDAANAPYAFLEGQAAMDVYALLAKYETHIYLENEIEYTV
                     TMRLLFPHEVLNADSTPIAIPTEARLPVNCTP"
BASE COUNT          590 a          555 c          525 g          541 t
ORIGIN      
        1 atgtgccacc aataaggttt ggcgtaattg agaacgcgta ataaaagctc gcgggtgact
       61 ttgggttttt cgtcgccttg gcgcatcgcc atgaaccagc cgtatccgtg cataaagggt
      121 gtctccgata attagggtag gggtgactcg tcaagatctt ggtgaggtga tggcgcgtca
      181 tgtatccttg catgaaaatt ggcttgtcac actcaggcgg gtcgccccta cggaatgatg
      241 tgactataag accaagtgac cattccggca actggacaaa gggggaggtt aatgaaagtc
      301 tgatgaagtg taaagcaggt gcaaagaacc ttttcaaaca aaccaataca atacatcaaa
      361 ctttaaatag gagcgattta atcagatgaa gttatacaaa cccacactcg tacttgtact
      421 aacaacctta cttgttgcct gcgcacctgc aacagaagaa gcttcacatg cttccactcc
      481 gcactggacg tatgaaggcg aagaaggtcc tgaacattgg ggcgaccttg acccatcgta
      541 tgcagcctgc gaaacaggta ccaagcaatc accgatcgat gtttccaacc cagcagaaca
      601 ggatttggcg aatattgctt ttcattatca gccaagcgaa gtcaatattc tgaacaatgg
      661 acataccgtc caagtcaact atgatgcggg cagttacatt gaattagatg gtgtccgcta
      721 tgatgtggct caattccatt atcatgcgcc cagtgaacac gccgtagatg ggaaactctt
      781 cgcagcagaa ttgcatatcg tccacaagaa tgcagatggc ggcttagcag tggtaggtat
      841 tttattagac gaaggcgcag aaaatgcagc ctatcaaccg ttcattcaaa acctcccagc
      901 agaagaaagt gacgtgaaag atgcaggcgt taccgtcaac gccgcagatt tgctccctgc
      961 tgtgcaaacg actttccgtt atagcggctc gcttaccaca ccaccttgta ctgaaggtgt
     1021 gaactggttg cttatgacta cacctgtgga aatctctgca agccagatcg aagcccttgc
     1081 ttctttattt gaagcaggca ataacagacc cgtccaaccc ttgaacgacc gtccgctggc
     1141 ggaagatagt acaccgtaac aagaattcaa gagacagtca cctttgaggt gactgtctct
     1201 tttaagggcg ggatataatt caagacaaat ttcatgcctc tacgatttcc tgtgacctcg
     1261 cttgttttga tatggatgtt ggttggctgt agttctttgc cttttgttgc cacgcccaca
     1321 cctacagcgc ttcccacctc taccccaacg cggactccac tccctacgca gacgccttcg
     1381 cctacggtta cacctattct cgtcacgtat ccgttcaagc atgtgtggtt taagtacaca
     1441 gtggagcgcc cggctgggga ggcgaatccg cttgggttga cggaagagcc gctgctcgtc
     1501 atctataccg atggcaccat gatcgcagcg cggacggggg atccgaaagt ccgcacgcgg
     1561 gttctcaaca cgaaagaagt ctgcgacttt ctcaaccggc tagatacctt cggtttttac
     1621 atgctggaag attcgcactc gacagatgag acaaatcctt tatatgattt tggcacaaaa
     1681 tatcaagccg tggatgaagg tgattttgcc agcctcactg tgaatcggac ggaccccaaa
     1741 agcgtaactt tctttgagcc ttatcgaaag ttttttgcaa gacccatgcg caagatcatg
     1801 gaatttgtag aaacgtacaa tcctggcgcg ctcaaggcat atgagccaga ccgtttggtc
     1861 gttttcgtgc aacgcggacg agccgaaggc accgatgaaa aagtccgcgt gcgtgattgg
     1921 acggtgaata ctttcagcct gaacgatgct gccaatgcgc cctatgcatt tctagaaggg
     1981 caagccgcca tggatgtgta tgcattgctg gcgaagtatg aaacccacat ctatcttgag
     2041 aatgaaatag aatacacagt cacgatgcgt ttgctgttcc cacatgaagt gcttaatgca
     2101 gactctacgc cgattgccat ccccaccgaa gcgcgccttc ctgtgaattg cacaccataa
     2161 cctttatttg aggaaaccca tgcctaccaa aaccccgtcc attatttcaa c
//
LOCUS       DAOQNJ010000482         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_40197_length_2211_cov_8.348794, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000482 DAOQNJ010000000
VERSION     DAOQNJ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40197_length_2211_cov_8.348794"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLG52_11920"
     CDS             complement(<1..848)
                     /locus_tag="PLG52_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNB42201.1"
                     /translation="MTDFYPEETTAPSSPGVPHSREAEEAVVGAVLINPEVYYDLAQF
                     LSADDFYIHRNRWIWDAFIRLHEARTPVDLLTLSEDLEKAGQLAEIGGSAYLTSLINQ
                     VPTSLNAEAYGHIVEENSLRRRMIQAANQIASLAYKGDGIDNVMGEAEKAVFGVSERR
                     TRHDVEPIRKVLSDYYDQVAELAKLGADFHGVPTGFIDLDKLLRGLHPSDLLIIAGRP
                     GQGKTGFLLSIAKNAGLTYKKHVAIFSLEMSNEQVVQRLIAQETGISSQNLATGKLAD
                     DEWKLF"
     gene            complement(864..1436)
                     /locus_tag="PLG52_11925"
     CDS             complement(864..1436)
                     /locus_tag="PLG52_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNB42202.1"
                     /translation="MSSEMVEVVIDSIRVHLMAPQRVVVLKQVNAERYLTIWVGPYEA
                     EAITVALQEVEMVRPLTHDLLKNVFKAFNARIVRVEIVKLEADIYFGNIVAEADGREI
                     HIDSRSSDAIAIAARAHAPIMVHPSVMESAGVVPEQDMPEAPITPARKETPPPPLPDD
                     ASDRLSVFKDFIEKLDIDNPDKDKPESPST"
     gene            1638..1712
                     /locus_tag="PLG52_11930"
     tRNA            1638..1712
                     /locus_tag="PLG52_11930"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1673..1675,aa:Pro,seq:ggg)
     gene            1806..2183
                     /locus_tag="PLG52_11935"
     CDS             1806..2183
                     /locus_tag="PLG52_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNB42203.1"
                     /translation="MQSNEPIYSSPTPNPPPTNRHSIVSLVLGLITLFLFCGGILIPI
                     PFTSFICVPISALIGLGAFIYGIISLNTIRKNNETGHPMAWSGILIGGFVFLCLICMI
                     VAILSLFNFAPQYVPPFLQGYQI"
BASE COUNT          519 a          578 c          547 g          567 t
ORIGIN      
        1 ttgaacaact tccattcgtc gtctgcaagt ttaccggtgg ctaaattttg tgaagagatc
       61 cccgtctcct gcgcaatgag acgttgcacc acttgttcat tggacatttc caaagagaaa
      121 atagcaacgt gctttttgta ggtcagcccg gcattcttgg caatggaaag caagaaacca
      181 gttttaccct gacccggacg ccccgcgata atcaacaggt ctgaaggatg caacccgcgc
      241 aacaatttat cgaggtcaat aaagccggtc ggcacgccgt gaaagtccgc gccgagtttg
      301 gcaagctctg ccacctggtc gtaataatcc gaaaggactt tacggatcgg ctccacgtca
      361 tgacgggtgc ggcgttcgct cacaccaaag acggctttct ctgcctcccc catcacattg
      421 tcgatgccat cgcctttata cgccaacgaa gcgatctgat tcgcagcttg gatcattctg
      481 cgccgcaggg agttctcttc aacgatgtga ccataggctt cagcgttgag cgaggttggg
      541 acttggttaa tgagcgaggt caggtaggct gagccgccaa tctctgccag ttgaccggct
      601 ttctccaaat cttcagagag ggtcaacaag tctacggggg tgcgggcttc atgcaaacgg
      661 atgaaggcat cccagatcca gcggttgcga tgaatgtaaa aatcatccgc cgagaggaac
      721 tgggcgaggt cgtaatacac ttcggggtta atcaacaccg cccctacgac ggcttcttcg
      781 gcttcccggc tatgcggtac acctgggctg gagggggcgg tagtttcttc ggggtagaaa
      841 tctgtcattg ggtgagatca gctttaggtt gagggtgatt cgggcttgtc cttgtcgggg
      901 ttatcgatat ccagtttttc gataaagtct ttgaagacgg ataagcgatc actcgcgtca
      961 tcgggcaagg gcggaggcgg cgtttctttg cgagccgggg taatgggcgc ttcgggcatg
     1021 tcctgttctg ggacgacccc cgcgctttcc atcacactgg gatgaaccat gatgggcgca
     1081 tgtgcacgcg cggcaatggc aatcgcatcc gacgagcgcg agtcgatatg gatctcgcgt
     1141 ccatccgctt cggcgacgat gttgccaaaa taaatatccg cttcaagttt gacgatctct
     1201 acgcggacga tgcgcgcatt gaatgctttg aatacgttct tgagcaggtc atgagtgagc
     1261 ggacgcacca tttccacttc ttgcaacgcc acagtgatgg cttccgcttc atacggtccc
     1321 acccaaatgg tgagataccg ttcagcatta acttgtttca gcaccaccac gcgctgaggc
     1381 gccattaaat gcacgcggat gctgtcgata actacttcta ccatttcaga cgacataggg
     1441 gactccaatg cgcttatttt agcataaagg ttgggggcat gcctcagatt aatataagag
     1501 gaagatatgt tttgttttgc aaggtagacc aaggatttag acgatagaag aaccgtttcc
     1561 atcgttagcc ataattcaag cctaaaattg ggtttgcatt tatcggcaaa gacgactata
     1621 atgcccctcg ctcaattcgg ggcgtggcgc agcccggcta gcgcacttgc atggggtgca
     1681 aggggtcgga ggttcaaatc ctctcgcccc gacagagtag taagaaaacc gtcctgccgg
     1741 ggacggtttt tatttttaac atcgtgttat tcgaagcgat aataaagaac ctctataatt
     1801 atcctatgca atcaaacgaa cccatttact catctccaac gcccaatccc ccacccacaa
     1861 acagacattc catcgtcagc cttgtccttg ggctaatcac ccttttcctc ttctgcggtg
     1921 gaattttgat tccgattcca tttactagtt ttatctgcgt tcccatcagc gccctgattg
     1981 gcttgggagc gtttatttat ggcatcatct cactcaacac cattcgcaag aacaatgaaa
     2041 ccggtcatcc catggcatgg agcggcatcc tcattggcgg atttgttttc ttatgcctga
     2101 tctgcatgat cgttgccatc ctatccctct tcaactttgc acctcaatac gtgccaccct
     2161 tcttacaagg ctatcaaatc taagcacttc ttaacaagta acttgtcaat c
//
LOCUS       DAOQNJ010000483         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_40703_length_2196_cov_6.820645, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000483 DAOQNJ010000000
VERSION     DAOQNJ010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40703_length_2196_cov_6.820645"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLG52_11940"
     CDS             complement(<1..324)
                     /locus_tag="PLG52_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42204.1"
                     /translation="MLFIALIVCILISTGSFVWGYSQAGYEDAVRWLVAFGVLWFLAE
                     WRKWKWFSAPAVLFSLFLAGYGVWFKFVPGLMFNGAVFSVLAWNITEFQEKLRLLPQR
                     EDKQGM"
     gene            382..921
                     /locus_tag="PLG52_11945"
     CDS             382..921
                     /locus_tag="PLG52_11945"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNB42205.1"
                     /translation="MPKEQEINSIERTAEECQGKLHPKAIVGLQLFNQGEFFEAHEEL
                     ETAWRDETSSIRDLYRGILQIAVTYLHITRGNYDGAVKVYGRSLKWLQGWGDICRGVN
                     IKKLRRDAETVMQEVTRLGWERIREFDRSLFQPIQWNEKRLWICDRCGSEMYEKNCKV
                     SCPTCGNRFDCSDLNLYFD"
     gene            937..1707
                     /gene="fabL"
                     /locus_tag="PLG52_11950"
     CDS             937..1707
                     /gene="fabL"
                     /locus_tag="PLG52_11950"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HNB42206.1"
                     /translation="MNFQNKIALVTGSGRGIGKAIALNFASQGADIVVNFFRNRAPAE
                     ETVREIEKLGRRALLVKADVGDLDDLNRLFDEVEKEFGGLDIFIHNAASGYNRPAMEQ
                     KPKGWDWTMNINARALLFAAQRATPLMEKRGGGSIVSISSAGSVRVLPDYVVVGASKA
                     ALEALTRYLGVELIQRGIKVNAVSPGVVETEALHHFSSMGGQENILQKFIERVPAGRL
                     ITPEDVAEVVAFLCSPAAAMIVGQTIVVDGGYTLPIQQ"
     gene            1716..>2196
                     /locus_tag="PLG52_11955"
     CDS             1716..>2196
                     /locus_tag="PLG52_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42207.1"
                     /translation="MFQAAPPTRFDLQFSIAGIPVRVHPLFWVIAILLGSGSSNFLMI
                     PVWIAVVFISILVHELGHAFAFRRFGQPSRILLHFSGGLTIPESMPWGGGYATVSLTP
                     NQHIFVSLAGPFAGFLLAGLVLVVGVAMGGTIIFSTLLKFIPFPIVMMPYGSGILTDI
                     "
BASE COUNT          576 a          522 c          521 g          577 t
ORIGIN      
        1 cataccttgt ttgtcttctc gttgaggtag tagcctcaat ttttcttgaa attcagtaat
       61 attccatgcc aatacactaa acacagcacc gttaaacata agcccaggaa caaacttaaa
      121 ccaaacgcca tacccagcta aaaacagact aaacagcaca gccggagcag agaaccactt
      181 ccatttacgc cattccgcca aaaaccaaag aacaccgaaa gctactagcc agcggacggc
      241 atcctcatag ccagcttgag agtatcccca tacgaacgag cctgttgata tcaagatgca
      301 gacgataagc gcaataaata acaattttcc tccaacacaa attttaccat tcgtataaaa
      361 cctgcatgtt atactctcga tatgccaaag gaacaagaaa taaattctat tgaaagaaca
      421 gcagaagaat gccaaggaaa gttacatccg aaagccattg taggtcttca gttattcaat
      481 caaggagaat tttttgaagc ccatgaagag ttggaaaccg cttggcgaga tgaaaccagc
      541 tcaattcgag acttatatcg cggcattctt caaatcgcgg taacctatct gcatattaca
      601 cgcggaaact atgacggtgc agttaaagtc tatgggcgga gcttgaagtg gttgcaaggc
      661 tggggcgaca tctgccgcgg tgttaacatc aagaaacttc gccgtgatgc agagaccgtt
      721 atgcaagaag tgactcgttt ggggtgggaa agaatacgtg agttcgatag gtctttattc
      781 caacctattc aatggaacga aaagcgcctg tggatctgcg atcgctgtgg cagtgagatg
      841 tatgaaaaga attgcaaagt gagttgccca acctgtggca accgcttcga ctgttcggat
      901 ttaaatcttt actttgatta atgctctgga gacccaatga attttcaaaa caaaatcgcc
      961 ctcgtcaccg gctcagggcg cggaattgga aaagccatcg ccctgaattt tgccagtcag
     1021 ggggcagata ttgtcgttaa tttctttcgc aaccgtgctc ctgccgaaga gacagttcgc
     1081 gagattgaaa aacttggcag gcgtgctttg ctggttaaag ccgatgtagg cgatttagat
     1141 gacttaaatc gcctcttcga tgaagtagaa aaagaatttg gcggcttgga tatcttcatt
     1201 cacaatgctg catcaggcta caaccgccct gcaatggaac aaaagcccaa aggctgggat
     1261 tggacaatga acatcaatgc gcgtgcattg ttattcgctg cacagcgtgc cacgcctctt
     1321 atggaaaaac gcggcggtgg gagcatcgtc agcatatcga gtgcagggtc ggtgcgagtg
     1381 ttacccgatt atgttgtggt gggcgccagt aaggcagccc tggaagccct cacgcgctat
     1441 ttaggtgtgg agttgattca gcgtggcatc aaggtaaacg cggtgtcacc cggtgtggtg
     1501 gagacagagg cgttgcacca tttttcatcc atgggcgggc aagagaacat cctccaaaaa
     1561 ttcattgagc gcgtccctgc gggtcgtttg atcacgcctg aagatgtggc tgaagtggtg
     1621 gcatttttat gctcacccgc cgcagctatg attgtgggtc aaacgattgt tgtagacggc
     1681 ggttatactt tacccattca acaataggat attttatgtt tcaagctgct ccccctactc
     1741 gctttgatct tcaattttca attgcaggca ttcctgtacg tgtacatccg ttgttttggg
     1801 tcatcgcaat tttgctgggt tctggctcaa gcaattttct gatgatccct gtttggatcg
     1861 ccgtagtgtt catttcgatt ctggttcacg aacttggaca tgcctttgca tttagacgct
     1921 ttggtcaacc ctcgcgcatt ctcctgcatt tctctggcgg attaaccatc cccgaatcga
     1981 tgccatgggg cggcggatac gccactgtaa gtttgacacc caaccaacac attttcgtct
     2041 cccttgccgg accctttgcc ggattcttgc ttgcaggctt ggtcctcgtt gtaggtgtgg
     2101 caatgggcgg caccatcatc ttttcaactc tgctcaagtt tatccccttc cctattgtaa
     2161 tgatgccata cggtagcggc atcctgacgg acatct
//
LOCUS       DAOQNJ010000484         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_40742_length_2195_cov_14.367757, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000484 DAOQNJ010000000
VERSION     DAOQNJ010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40742_length_2195_cov_14.367757"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLG52_11960"
     CDS             complement(<1..217)
                     /locus_tag="PLG52_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNB42208.1"
                     /translation="MLKKLKEQVFQANLLLPKHGLVTFTWGNVSGIDREKGLVVIKPS
                     GVPYETMKRDDMVVLELASGKVVDGELK"
     gene            complement(274..1875)
                     /locus_tag="PLG52_11965"
     CDS             complement(274..1875)
                     /locus_tag="PLG52_11965"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNB42209.1"
                     /translation="MDKNEIKKAIESGKTSLGIELGSTRIKAVLIAEDHSPIASGSYE
                     WENQYEHGVWTYSLDMVWKGLQESYRNLSKEVSEKYGVTLTTIGAIGFSAMMHGYLAF
                     DKDGKQLVPFRTWRNTMTGQAAEKLTDLFQFNIPQRWSIAHLYQAILNKEAHVKDISH
                     QTTLAGYVHWKLTGQKVMGVGEASGMFPIDSTINDFDKKMLGQFNDLLKAEKITWTLQ
                     DILPKVLVAGESAGALTKEGAKLLDPSGGLKAGIPLCPPEGDAGTGMVATNSVAVRTG
                     NVSAGTSVFAMIVLEKALSKLYPEIDMVTTPTGKPVAMVHANNCTSDLNAWVGLFGEF
                     TKALGVEISQSKLFETLYKMALTGDADGGGLLAYNYLSGESITDLTEGRPLFIRTPES
                     RFTLPNFMRTHLFSSLGTLKIGMDILSQENVKLDQLLGHGGFFKTEGVGQKIMAAAMN
                     TSVSVMETAGEGGAWGMALLAAYVLNKAGSETLEGYLNKKVFANEKSSTIAPDKKDVD
                     GFNTFMARYKAGLGIERAAVEGMKI"
     gene            complement(1963..>2195)
                     /locus_tag="PLG52_11970"
     CDS             complement(1963..>2195)
                     /locus_tag="PLG52_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42210.1"
                     /translation="CMDEPVDYGEMGKYLHETRGLPTVEIKTEYHSTWLDNAKAKFLL
                     NWRPEYDLKKMIDSAFDYARAEDDPRKVWYPG"
BASE COUNT          480 a          569 c          535 g          611 t
ORIGIN      
        1 gcttgagttc gccatctacc actttgcctg acgccaactc caaaacaacc atgtcgtcac
       61 gtttcatcgt ctcataggga acaccagaag gtttgatgac gaccaatccc ttctcgcggt
      121 caatgccaga gacgttgccc caggtgaagg tgacgagtcc gtgcttgggt aataaaagat
      181 tggcttgaaa tacttgttct tttagttttt tgagcatggt ttctccgaaa atcgatactc
      241 gatattcgtt cttcgaatgt cgagtatcga atatcaaatt ttcatcccct ccactgcagc
      301 acgttcaata ccaagacctg ctttgtaacg tgccatgaat gtgttgaaac cgtccacatc
      361 ctttttgtca ggcgcaatgg tggagctttt ttcgttggca aatacttttt tattcaaata
      421 accttcgagc gtttcactgc ctgctttatt gagcacatac gccgccagca gagccatgcc
      481 ccatgcaccg ccttcgcctg cggtttccat caccgagacg gaggtgttca tcgctgctgc
      541 catgatcttt tgaccaacgc cttcggtttt gaagaacccg ccatgcccca acaattggtc
      601 aagctttacg ttttcctgag agagaatgtc catgccaatc ttcaacgtac ccagtgacga
      661 aaacaaatgc gtgcgcataa agtttggcaa agtgaagcgg ctttcaggcg tgcggataaa
      721 taaagggcga ccttcagtta agtccgtgat ggattcacca gagagatagt tataggcgag
      781 caatccaccg ccatctgcat cacctgtgag cgccatttta tatagggtct caaataattt
      841 cgattggctg atttccacac cgagcgcttt cgtgaattcg ccgaacagcc ccacccatgc
      901 gttgaggtca gaggtgcagt tgttggcgtg aaccattgcc acaggttttc ctgtgggcgt
      961 ggtgaccatg tcaatctcgg gatagagttt ggataacgct ttttccaaca cgatcatggc
     1021 gaagacggat gtccccgccg agacgtttcc ggttcggact gcaacgctat tggttgccac
     1081 catgcctgtg cctgcatctc cctcaggcgg gcagagcgga ataccagcct tgagtccgcc
     1141 gctggggtcg agcagtttgg caccttcttt ggtcagtgcg ccagcggatt cgccagcgac
     1201 caaaactttg ggcagaatgt cttgcaatgt ccaggtgatt ttctctgctt tgagcaagtc
     1261 gttgaattgc ccaagcattt ttttgtcaaa gtcgttgatc gtgctgtcga tggggaacat
     1321 gcctgaggct tcacctacgc ccatcacctt ttgccccgtc aacttccagt ggacatatcc
     1381 cgccagcgtg gtctggtgac tgatgtcttt gacatgcgct tccttgttca atatcgcctg
     1441 atacagatga gcaatgctcc aacgctgcgg aatgttgaat tggaagaggt ccgtgagttt
     1501 ctcagcagct tgtcctgtca tcgtattgcg ccaggtgcgg aaggggacaa gttgtttgcc
     1561 atctttgtca aacgccagat agccgtgcat catggcgctg aaaccgatcg cgccgatggt
     1621 ggtgagcgtc acaccgtatt tctccgaaac ctctttactg agattgcggt aactttcttg
     1681 caagcctttc cacaccatgt ccaagctgta cgtccagacg ccatgctcat attggttttc
     1741 ccattcgtag cttcccgatg caatggggga atggtcttca gcgatcaata ccgctttgat
     1801 gcgggttgag ccgagttcaa tgccaagaga ggtcttgccg ctttcgattg cttttttgat
     1861 ttcgttttta tccatatgtg tctcctcatg tcaccctgag cgatagcgaa gggtctctac
     1921 gattatctaa gagatgcttc gctatcgctc agcatgacaa aattatccgg gataccaaac
     1981 cttgcgtgga tcatcctcgg cgcgggcgta atcgaacgcc gagtcgatca tctttttcag
     2041 atcatattca ggtcgccagt tgagtaaaaa ttttgctttg gcattgtcga gccatgtgga
     2101 gtgatattcg gtcttgatct ccacggtggg caagccgcgc gtctcgtgca gatatttgcc
     2161 catctcgccg tagtccacgg gttcgtccat gcaga
//
LOCUS       DAOQNJ010000485         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_40745_length_2195_cov_10.454673, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000485 DAOQNJ010000000
VERSION     DAOQNJ010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40745_length_2195_cov_10.454673"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="PLG52_11975"
     CDS             <1..1565
                     /locus_tag="PLG52_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42211.1"
                     /translation="LNTLTADLPGPAKQILGAALATAMGAAGGGAAGAVGAFNEDMNN
                     RQLHMPEKWLALKLRAKAKTIGLNYSLSEIEDALRWSNFKGENASSNTYINTTKDALL
                     KGLVGDIYASKVFDKMIPGVNTDARITYIFAGTEEGQPTLIQNMNQIPKPSRELIAFI
                     QSNSKGYSWDESSIHMEETEKNPITAWTSEGYVRITDPSTAEDSLNHQSITINNSALT
                     IPTAFCPPSNCTANSSIALSSTNARDTQLLQTYSNEIEREGEKKFVRLGVSIGGAYLT
                     PTSIPSLLLTSTGLAAIESSLSQLIDGPWSARQTLTDTAINSGIALATITTGLIGTKI
                     LNNIIETQSPSRIQDLIQNSTLTEKKLLSPTSLPPPVAKEHISSTPNNEPLHYDIANW
                     ADYGLPSDGYFVRTLSPQQYRDIASGRQISFSGKPTEAYPEGMGFIGAAEEVRFIKSG
                     EEYRKALKLDYTPTFLLEFQLADPAGLQNVLSAPYNEFVRGGLTGAGFREWNLPGISS
                     THIINPKPIILK"
     gene            1562..1864
                     /locus_tag="PLG52_11980"
     CDS             1562..1864
                     /locus_tag="PLG52_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42212.1"
                     /translation="MMLTKYTTANNQQKFIDLDSVIDVVKGESGIKTGWTALIIYDPT
                     SKKFVELRDSPQDYQGNSMDEAEEVDEAYLINTFKIPRSALADLYSAPTEWKTIIQ"
BASE COUNT          743 a          599 c          432 g          421 t
ORIGIN      
        1 ccctcaacac cctcaccgca gacctaccgg ggcccgccaa acagatcctc ggcgccgcac
       61 tggccaccgc gatgggggcg gcaggcgggg gcgcagcggg agcggtgggg gcgttcaatg
      121 aggacatgaa taaccggcag ttgcacatgc ctgaaaaatg gctggcctta aagctaagag
      181 ccaaagccaa gacaatcggc ttgaattact ctctatctga aattgaggat gccctcagat
      241 ggtctaactt caagggcgaa aacgcaagct caaacacata catcaacaca acaaaagacg
      301 cattgcttaa agggctcgtt ggcgatatat acgcatcaaa agtttttgac aaaatgattc
      361 cgggagtcaa caccgacgca cgcatcacct acatatttgc aggcaccgaa gaaggacagc
      421 caacgttaat tcaaaacatg aaccaaatcc caaaaccatc cagggaatta attgcattca
      481 tccaatcaaa tagcaaaggc tattcatggg acgaatcaag cattcacatg gaagaaacag
      541 aaaaaaaccc aattacggcc tggaccagcg aaggatacgt tcgcattaca gaccctagca
      601 ctgccgaaga ctcgctcaac caccaatcga ttaccataaa caattcagct ctaaccatac
      661 caaccgcatt ctgcccaccc agcaattgca cagcaaacag cagcatcgca ttgtcaagca
      721 ccaatgcacg agacacgcag cttttacaaa catattcaaa tgaaatagaa agagaaggcg
      781 aaaaaaaatt cgtaaggctc ggagtcagca ttggcggagc gtatttaaca ccaacttcca
      841 ttccttccct gctattgaca tcaacagggc ttgcggcaat tgaaagctcc ctaagtcaat
      901 taattgacgg gccgtggtca gcaagacaaa ccctaaccga cacggcaata aactctggga
      961 ttgcactagc aacaattacg accgggctaa ttggaacaaa aatcctaaac aatatcatcg
     1021 aaacacaatc cccatccaga attcaagatt taattcaaaa cagcacactt accgaaaaaa
     1081 aactcctatc ccccacatca ctaccgcccc ccgtagcgaa ggaacacatt tccagcacgc
     1141 caaacaacga gccactccac tacgacatag ccaactgggc agattatggt cttccctcag
     1201 atggatattt tgttcgaacc ctatctcccc aacaatatcg tgacatcgct tccggacgcc
     1261 aaatttcatt ctcaggaaag ccaacggagg cttatcctga gggaatggga ttcattggag
     1321 cagccgaaga agttcgcttc ataaaaagtg gagaggagta tcgtaaggca cttaaactag
     1381 attacacacc aacatttctt ttagaatttc aacttgcaga ccccgccggg cttcaaaatg
     1441 ttctttcagc tccgtacaat gaattcgtac gaggtgggct taccggggcc ggctttcgag
     1501 aatggaacct tccaggaatt agctcaacgc acataatcaa cccaaaaccc attattctaa
     1561 aatgatgctc acgaaataca caaccgcaaa caaccagcaa aaattcatcg acctcgactc
     1621 cgtgatcgac gtcgtaaaag gagaaagcgg aataaaaacc ggctggacag cccttattat
     1681 ttacgaccca acatctaaaa aattcgtgga actacgtgac tcaccccaag attaccaagg
     1741 aaactcaatg gacgaagcag aagaagtaga cgaagcttac ttaataaaca cctttaaaat
     1801 cccaagatct gcactcgctg acttgtactc agcacccacg gaatggaaaa ccatcatcca
     1861 atagccgccc tagaacaatc gccccgcgcc tcaccccgaa caggccgaac gcacagaggt
     1921 tcttgttctc ttaattctga taaggatata ggggcgggtg gtctgcggcg aggacgactg
     1981 gacctcgatc aggagaccta ggataggata tcgaacaacc aaacacaaac tctggaacgc
     2041 tcataaatcc atttgagcta tctaaaacca gataaacatg aacccagaga aaacaaaaac
     2101 cgcagcccac aacctagaac gcaccatatc aaaatacgtg caaacggatg aagaagtcgc
     2161 caattttcaa aaaatactcc gccccctcat aaatc
//
LOCUS       DAOQNJ010000486         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_41016_length_2187_cov_11.591463, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000486 DAOQNJ010000000
VERSION     DAOQNJ010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41016_length_2187_cov_11.591463"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            457..1011
                     /locus_tag="PLG52_11985"
     CDS             457..1011
                     /locus_tag="PLG52_11985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB42213.1"
                     /translation="MQKIHFREAVLQDVDLIMKMEVEGFAPGNRERKEVYEQRIDVFP
                     QGSLMAYVGSECVGCIFSEIWHYAPKPAVEYFALGHDISSRHDSVSGTELYVTSMTIA
                     PPYRGLGLGAQLLSGCVRYVAQRFSQVTTVLLLVNETWGHARDIYVAAGFKEVVIFKN
                     FFRPVEWCCEDGIVMRRSLSGFPE"
     gene            1023..1508
                     /locus_tag="PLG52_11990"
     CDS             1023..1508
                     /locus_tag="PLG52_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42214.1"
                     /translation="MEEITVVTAFVAALSFAGGVVSNLLSSVLYDFSVGRLSFRSRDY
                     PSLVGTWKAQVLLEGVDPFEEIIIIKGQINRRFKGVYTSPATGRLKSMGVVELEIEGF
                     LLDHKTIRYVAQSSRNQHVDYVTGMAVISNDRCRIDGLSISLGVLTEEPTLAKVVFLR
                     V"
BASE COUNT          458 a          348 c          650 g          731 t
ORIGIN      
        1 ggactacgca ggtgcgggga agctcattga tagttgcgat tcgaatgttg gggacgcgga
       61 ttgagggttt tggctatcgg ggaaggacta cggggtggtg gggaaggatg ctgacggtca
      121 gggtggcggc gggaagcggc gcgccatcgg tatagagcgt cagggcgtct gatcgaaatc
      181 tcgaaatcga cgccttgttg tgctagtgac agtcaggaga tcatatgagc tagacactga
      241 gtagattggt gcttatgctg atcgtgttgt gtagaaaaaa gttcgtgaaa tttataatgt
      301 ttttgtgcaa aatatcaagc tgagtggcta aaggtccgtg tttttttgat tgtttataag
      361 aatatcgatt ttgtgggccg tgaagttgag ctgcgttgct gtggtcggtc atgcgttggt
      421 ctgattaaat tttaattttg tgttgttgat gtttctatgc aaaaaataca tttcagagaa
      481 gctgttctcc aggatgttga tctcatcatg aagatggagg tggaaggttt tgccccagga
      541 aatcgtgagc ggaaggaggt ttacgagcaa agaatagatg tatttcctca ggggtcgctt
      601 atggcgtatg ttgggtcgga gtgcgtgggt tgcatttttt cggagatatg gcattacgcg
      661 ccaaagcctg cggttgaata ctttgctctt gggcatgata tcagttctcg tcatgattcc
      721 gtcagtggga cagaattgta tgtgacatca atgacgattg cgccgcctta tcgtggactt
      781 ggtttaggtg cgcaactact ttctggatgt gttcgatatg ttgcgcagag gttttcgcaa
      841 gtgacgacag tgctattgtt ggttaatgaa acttgggggc atgctcgaga tatctatgtt
      901 gcagcagggt ttaaagaggt tgtgattttt aagaattttt ttcggccggt ggagtggtgc
      961 tgtgaggatg gaatagttat gcggcggtcg ttgagtggat ttccagaata gtgagggtgc
     1021 tgatggagga gattacggtt gtcaccgcct ttgttgcggc tctttctttt gctggtggag
     1081 tagtctcgaa tcttctttcg tctgttttat atgatttttc agttggacga ttgtcatttc
     1141 gctccaggga ttaccccagt cttgttggta catggaaagc tcaggttttg cttgaagggg
     1201 ttgacccgtt tgaagaaatt ataattatca aagggcagat taatcgacga tttaagggcg
     1261 tgtatacctc tccggcaacg gggcgactga aaagtatggg ggttgttgag ctcgagattg
     1321 agggttttct tcttgatcat aagacgattc gatatgtggc acagtctagt cgcaatcagc
     1381 atgttgatta cgttactggc atggctgtta tatcaaatga tcgttgtcgg attgatgggc
     1441 tttctattag tcttggcgtt cttacggagg aacctacttt ggcgaaggtt gtgtttttgc
     1501 gtgtttaatg aattgctgag gctggtgctg tatttgtggt gtgttgttga tgagtttgtg
     1561 gttgatgttg gggtgctgtt ggcttaatga agtatctaag ggtgattgta aggctctgtg
     1621 gtggtgtctt tgtaagatga tgagagggca agtgtctagg taagtaggtt ttttgttatt
     1681 ggttttcccg gcggcaattt ttgtttttgt ggcgggcgaa aatgcaatgg ttgtgggtcg
     1741 tgctgctgct aagagattta ttgatgtggt tgtcgttggt gttgtaattt tttgattaaa
     1801 aatgcgccga tgtaaggctg caaaatattt ggtttgattt tgattgtctg atgcttttgt
     1861 gattggttta atgtttggcg ggatggtgca aaatttatat ttcgtctaag gttgtgacga
     1921 caaataagtt tttcaagatg agggtcttgg aaattcatgg cgttacaagc cgatattttt
     1981 cactgtttgc atttttgtct caggaagaga gttttgtctc ggcctgttag agcgcacttc
     2041 gctatttctg ctccaaagac taccccatgc caaatctttg ctgcctcgca ctgatgatgc
     2101 atgcggctgt ggccggcatt gcactctttg tcatacccgc ccccgccctt gccttcgact
     2161 tcaccggcac caagtccctc atcgccg
//
LOCUS       DAOQNJ010000487         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_41140_length_2183_cov_9.516447, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000487 DAOQNJ010000000
VERSION     DAOQNJ010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41140_length_2183_cov_9.516447"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLG52_11995"
     CDS             complement(<1..811)
                     /locus_tag="PLG52_11995"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB42215.1"
                     /translation="MSDRLERFRFDEQTPDRLDKFLVSHLPEFSRARIQGLIADGFVL
                     VNNVPAKKAGQWIEDGDDIEVRIPPPQPSGLVAEDIPLNIVFENDDLIVVNKPAGMVV
                     HPAAGHYSGTLVNAVLGYDPDLEGIGGEERPGLVHRLDKETSGLIILAKNERAHNWLQ
                     DQFRLRKVEKTYLALVDGKPPTPAGRVEAPIGRDPSHRKKMAIVSPGKGREAVSEYKT
                     LESFKNHTLLEFHPHTGRTHQIRLHCQFLGCPIVGDAIYGKRTVTLTIDRHF"
     gene            complement(804..1352)
                     /gene="lspA"
                     /locus_tag="PLG52_12000"
     CDS             complement(804..1352)
                     /gene="lspA"
                     /locus_tag="PLG52_12000"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNB42216.1"
                     /translation="MNEEMNVQEKKQPRNRRFLILFIVADLIIALDQWTKWWVIQNIP
                     LNTSWLPDSLNWLAPYARLLHIKNTGASFGMFQNSNIAFIVLTVLIVGGIIYYISTLE
                     HANDWMWVAAGLYLGGAVGNLIDRFLIGAVTDFVSVGTFFIFNVADASINVSVALLII
                     LGILESRASQSAPTTDSETRHE"
     gene            complement(1358..1888)
                     /gene="lspA"
                     /locus_tag="PLG52_12005"
     CDS             complement(1358..1888)
                     /gene="lspA"
                     /locus_tag="PLG52_12005"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNB42217.1"
                     /translation="MNSKVKDYMALIGVAGLSVALDQWTKWLVRENIEFGGQWLPEWL
                     NWLSPYARFVHWNNSGAAFGMFQNGNLVFTILAFVVIGAILYYFPQVEHEGWLLKFAM
                     GLQLGGAMGNLIDRLMMQKVTDFISVGTFPVFNIADASISVGVAVLLLDVWLKERAEK
                     LKAANEPKNEIIEEGQ"
     gene            complement(1915..>2183)
                     /locus_tag="PLG52_12010"
     CDS             complement(1915..>2183)
                     /locus_tag="PLG52_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HNB42218.1"
                     /translation="YADGYFRRMTNQGRVLIKGRSYSQVGRICMDQFMVDAGEDDVKV
                     GDEVVLLGGGIAPEELADWAGTNEYEVMTNISARVPRVFVAGVQ"
BASE COUNT          574 a          617 c          474 g          518 t
ORIGIN      
        1 aaaaatgacg gtcaatcgtc agcgtaacag tgcgttttcc ataaatcgca tcaccaacaa
       61 tcggacagcc caaaaactga cagtgcaaac gaatctgatg cgtccgcccg gtgtgtggat
      121 gaaattccaa caaggtatgg ttcttgaacg actcgagagt cttatattcg ctgacggctt
      181 cgcgtccctt gccaggagag acaattgcca tcttcttacg gtgactcggg tcacgcccaa
      241 tgggtgcttc cacgcgtccg gcaggtgtgg gcggtttgcc atcaaccaat gcaaggtagg
      301 tcttttccac cttacgcaag cggaattgat cttgcagcca gttatgagcg cgttcatttt
      361 tcgcaaggat gattaacccc gaagtttctt tatccaatcg atgtaccaag ccggggcgtt
      421 cttctccgcc aatgccttca aggtctgggt catagccaag aaccgcattg accagtgtgc
      481 cagaataatg tcccgccgcc gggtggacga ccatccctgc cggtttgttg accacgatca
      541 gatcatcgtt ctcaaaaaca atatttaagg gaatatcctc tgccaccaaa ccgctgggct
      601 gaggcggcgg gatgcgcact tcaatatcat cgccatcttc gatccactga cctgccttct
      661 tggcagggac attgttgacc aagacgaatc catcagcgat cagaccttga atacgggcgc
      721 gggaaaattc cggcaggtgt gagacgagaa atttatccag ccgatctgga gtctgttcat
      781 caaagcggaa gcgttcaagg cgatcactca tgcctggtct ccgaatcagt tgtcggcgcg
      841 gattgcgagg cgcggctttc caaaattccc aaaatgatga gcagcgcgac actgacgttg
      901 atgctcgcat ctgccacgtt gaaaataaag aatgtaccca cggaaacaaa atctgtcacg
      961 gcaccgatca ggaagcggtc gatcaaattg ccgaccgcgc cgccgagata caagcccgcc
     1021 gccacccaca tccaatcgtt ggcgtgttca agcgtggata tgtaataaat gatgccacct
     1081 acaattaaaa ccgtaagcac aataaaagcg atgtttgagt tctgaaacat accaaacgac
     1141 gcaccagtat tcttgatgtg cagcaaacgc gcatagggtg caagccaatt caatgagtcg
     1201 ggcagccatg aagtattcaa cgggatattc tggatgaccc accactttgt ccactggtcg
     1261 agggcgatga ttagatcggc aacgataaat aaaatcaaaa agcgcctgtt gcgaggctgc
     1321 tttttctcct gcacattcat ttcttcattc atcaacttta ttgaccttcc tcaataattt
     1381 cgtttttcgg ttcattggct gcttttaact tctctgcgcg ttccttgagc cacacatcca
     1441 gtagtagtac agccacaccc actgagatgg atgcatcggc aatattgaat acggggaatg
     1501 ttccaacgga aataaaatct gtcactttct gcatcatcaa acggtcgatg agattcccca
     1561 ttgcgccgcc caactgcaat cccatcgcga attttaatag ccaaccttcg tgttccactt
     1621 gcggaaaata atagaggatc gcaccaatga ctacgaatgc caaaattgta aacaccaaat
     1681 ttccattttg gaacattcca aatgcagctc cgctattatt ccaatgcaca aaacgcgcat
     1741 acgggctgag ccaatttaac cactctggca gccattgtcc cccgaattct atgttttcac
     1801 gaaccagcca tttcgtccac tgatcgagcg cgacggacaa acccgccacg ccaatcagcg
     1861 ccatataatc ttttactttg gaattcaact ctcacctcaa aacaaaaagt gcgcctattg
     1921 taccccagcc acaaacaccc tcggcacacg cgcgctgatg ttggtcatca cctcatattc
     1981 gttcgtgcct gcccagtctg ccagctcctc cggcgcaatg ccaccgccaa gcaaaaccac
     2041 ctcatccccc accttcacat cgtcctcgcc cgcatccacc atgaactgat ccatgcaaat
     2101 gcgccccact tgcgaatacg atcttccttt gatgaggact cgtccttgat tcgtcatgcg
     2161 gcggaagtag ccatcggcat atc
//
LOCUS       DAOQNJ010000488         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_41178_length_2182_cov_6.425952, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000488 DAOQNJ010000000
VERSION     DAOQNJ010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41178_length_2182_cov_6.425952"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PLG52_12015"
     CDS             <1..689
                     /locus_tag="PLG52_12015"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNB42219.1"
                     /translation="DVAVCCVPNAGRIYPYYVKSMWLRTPKGLLCALYGASELSTEVN
                     GAKVHIVEQTNYPFVLSIAFSIDVSQPVEFELAFRKPAWAIGFDIHTDGKWQEENGVI
                     KVHKTWQAGDTLVLQFQAKVKTDEFHKDEYFISYGPLVFARPLNGQAKEGRSYPVEGL
                     RDLYYSLDDSSSLADLPDTKDVRLEQQPFHAENPWETLHITIGSKTSSKLVPMGASIL
                     RQITFRKSIT"
     gene            694..2067
                     /locus_tag="PLG52_12020"
     CDS             694..2067
                     /locus_tag="PLG52_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HNB42220.1"
                     /translation="MAQSKTSISKSGTFVSQRPPVEKRNFVSPAVEATIATVSQAIAD
                     PELAWLFENCFPNTLDTTVKYSTINGKPDTFVITGDIEAMWLRDSTAQVWQYVPLINT
                     DANLKDLIAGVIYRQTQCILIDPYANAFNDGPTGSDWETDLTTMRKELHERKYELDSL
                     CYPVRLAHHYWKTCGDASIFDADWQVATHLIVKTMREQQRWDGPGPYRFQRVTSVSHD
                     TVPLGGFGNPTRPNGLIHSAFRPSDDACIYPFLIPSNFFAVRTLEQLAEIYSTQLKDS
                     AFADECTSLAKEVHSALGNFAIAQHPTHGAIYAYEVDGFGNALMMDDANIPSLLSLPY
                     LGAVSSNDPLYQSTRRFVLSLDNPYFFKGHAAEGIGGPHIGLGMIWHLSIIMRALTSK
                     DENEIIHCLSMLKHTHAGTGFMHESFDKEEPSQFTRKWFAWANSLFGELILKTYNERP
                     RILKMLL"
BASE COUNT          595 a          572 c          476 g          539 t
ORIGIN      
        1 aagacgtagc cgtgtgctgt gtccccaacg ccgggcggat ctacccctat tacgtcaagt
       61 ccatgtggct gcgcacaccc aagggtttgc tttgcgccct gtatggagcc agcgaactga
      121 gcaccgaagt caacggcgcg aaggtccata tcgtcgagca aaccaattat ccttttgttc
      181 tatccattgc cttttcaatt gatgtctcac aaccagttga attcgaactc gcttttcgca
      241 aacctgcctg ggcaattggg tttgatatcc ataccgatgg caaatggcaa gaagagaacg
      301 gtgtgataaa agttcataaa acatggcaag ctggcgatac gcttgtcctg cagtttcaag
      361 ctaaagtcaa aaccgatgaa ttccacaagg acgaatactt cataagttat ggccctttgg
      421 tatttgcccg tccgttaaac ggtcaagcca aggaaggcag aagttatcca gttgaaggtt
      481 tgcgagacct ctattattct ctggatgatt cttcaagctt ggctgatctt ccagatacaa
      541 aagacgtgcg gctcgagcaa caacccttcc atgcagaaaa tccgtgggaa actcttcata
      601 tcactatagg ctccaaaaca tcatccaagt tagttccaat gggagcaagc atcttgcgtc
      661 aaatcacatt ccgaaagtcc attacataac tttatggctc agtcgaagac atccatctcc
      721 aagtcgggca cattcgtttc acaaagacca cccgttgaaa agcgtaactt tgttagccca
      781 gccgtcgaag cgacgattgc aacggtctct caagccatcg ctgaccccga actcgcctgg
      841 ctgtttgaga attgctttcc aaacactttg gataccaccg ttaaatattc gactatcaat
      901 ggaaagcccg atacctttgt catcacaggg gatatcgaag ccatgtggct gcgcgactcg
      961 acggctcagg tctggcagta tgttccgctt atcaatacag acgcaaatct gaaagacctg
     1021 atcgccggag tgatttatcg ccagactcaa tgcattttga tcgacccata cgccaacgcc
     1081 ttcaatgatg gacctacagg cagtgactgg gagaccgatc tcaccaccat gcgaaaagaa
     1141 ctgcatgaaa gaaaatatga gcttgactcg ctgtgctatc cggttcgact cgcccatcac
     1201 tattggaaga cctgcggcga cgcctcgatc ttcgacgcag actggcaagt agctacacat
     1261 ctaattgtaa aaacaatgcg tgagcaacaa cgctgggatg gtcccggtcc gtatcgcttt
     1321 caacgcgtca catccgtctc acacgacact gtgccactag gcggtttcgg caacccaacc
     1381 cgaccgaatg gacttatcca ttccgccttc cgcccatccg atgatgcttg catctatcca
     1441 ttcctaatcc cgtcgaattt ctttgccgtg cgcacgctgg aacaactcgc tgaaatttat
     1501 tccacgcaac tcaaagactc agcatttgcg gatgaatgta cgtcccttgc aaaggaagtc
     1561 cattcggcat tgggcaactt tgcgattgct cagcacccca cgcacggcgc tatctatgca
     1621 tacgaagtag atgggttcgg taacgccttg atgatggatg atgccaacat tccaagcctg
     1681 ttatcgcttc cctatctcgg agccgtttca tcaaacgacc cgctctatca atccacgcgt
     1741 cgattcgttt tgagcttgga caatccctat ttcttcaaag gtcatgccgc tgaaggcatt
     1801 ggcggaccac acatcggtct gggcatgatc tggcacctga gtattatcat gcgcgcccta
     1861 acaagcaagg atgaaaatga gatcattcat tgtctatcca tgctcaaaca tacacatgcc
     1921 ggaacaggtt tcatgcacga gtctttcgat aaagaagagc caagtcaatt cactcgcaag
     1981 tggtttgctt gggcgaactc attattcggt gaattgattc taaagacata taacgaacgt
     2041 ccgcgcattt tgaagatgct attataacaa cgcaaaagac actacatatt cctttttcac
     2101 agaagacgtt tcaggagata ccatggataa cgatctagaa acaatgacga aagaagagtt
     2161 aatggctgaa gttaggaaac tt
//
LOCUS       DAOQNJ010000489         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_41396_length_2175_cov_6.841038, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000489 DAOQNJ010000000
VERSION     DAOQNJ010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41396_length_2175_cov_6.841038"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PLG52_12025"
     CDS             complement(<1..1221)
                     /locus_tag="PLG52_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42221.1"
                     /translation="MAQRILRGLEITILLFVLAALTGYSNPSLTNPIEKVRAYTRSLE
                     FDYVEWMANAAVLKMRAASINLPQTLNREEQKQVVMEYFKTTQFILEKEYLLSQLYAD
                     PSIQDKDAATETVRADLAELYKRQEQIAPLAEAILQDQVTQVLVEQGLTVGGQPIPNV
                     WYHSTPLPMALIISPRDHIEQTANISVNTDLTVDEQVKLEEDVAKGLDVSSLVVQIGG
                     VGVYPTMVARTTNLNWLLSTIAHEWIHNYLTQRPLGLLYGESPELRTMNETTASIAGD
                     EIGAIVIEKFYPELKPAASFPNLSLISLSFDRPDPETLERPPFDFRAQMHETRVTADA
                     LLAAGKIDEAEAYMEARRQIFLQNGYLLRKINQAYFAFHGAYADVPGGAAGEDPVGPA
                     VRALREQSPSLKDFI"
     gene            complement(1245..1706)
                     /locus_tag="PLG52_12030"
     CDS             complement(1245..1706)
                     /locus_tag="PLG52_12030"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB42222.1"
                     /translation="MTSNTSLNNSTIFQKSVEIAATTSKVWMLLTEPEQMKKWMMPDI
                     ELSIQTDWEVGSPIFIRGTMSGKEFENKGLVLKFEPEKNLQYSHLSSVSHLPDRAENY
                     SILDFQLHSIHQKTQLMFTLRNFPTESIYKHMAFYWNVTLEVFKRIAEEQR"
     gene            complement(1713..>2175)
                     /locus_tag="PLG52_12035"
     CDS             complement(1713..>2175)
                     /locus_tag="PLG52_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42223.1"
                     /translation="RAQTTKTEIAPSPTQSAITDEKTVLQEDLSQILGVDRKNTPRLT
                     KIAYSTPENGDITITWSLQRYSTPALTESNAQIDAVNILKTLKKNKTRYIYVILIGMF
                     PPSIANNNSTEQEVLNLGFNKSRLDKVNLEEFPSSDVFNLADVAFIADDWR"
BASE COUNT          477 a          442 c          599 g          657 t
ORIGIN      
        1 gatgaaatct ttgagtgagg ggctttgttc gcgcaaggca cgcacggcgg gaccaaccgg
       61 gtcttcaccg gcggcgccac cgggcacgtc tgcataggcg ccgtggaagg cgaagtaggc
      121 ttggttgatc ttacgaagga gatagccgtt ctgtaaaaag atttgacggc gcgcttccat
      181 gtaggcttct gcttcgtcga ttttgccggc tgctagtagg gcatcggcag tgacgcgggt
      241 ttcgtgcatt tgcgcgcgga agtcgaaggg cgggcgctct aaggtttcgg ggtcggggcg
      301 gtcaaaggaa agcgagatca agctcaagtt cgggaaagag gcggcgggtt ttaattccgg
      361 gtaaaacttt tcgatgacta ttgctccaat ttcatcgcca gcaatcgatg ctgtcgtttc
      421 gttcatggtg cgcaattcgg gagattcgcc atataacaac ccgagcggac gttgcgtgag
      481 gtagttgtgg atccattcgt gagcgatggt gctgagcagc cagttgaggt tggtggtgcg
      541 tgccaccatg gtggggtata cgcctacgcc gccgatctgc acaacaagcg acgagacatc
      601 taaccccttt gcaacatctt cttcaagttt gacttgttca tcaactgtga ggtctgtgtt
      661 gactgaaata ttagcggttt gctcgatatg gtcacgtggt gagatgatga gcgccatagg
      721 caggggcgta gagtgatacc agacattcgg gatgggttgc ccgcccacgg tgagaccttg
      781 ttcgaccaat acttgtgtga cttggtcttg caagatggct tctgccaacg gcgcgatttg
      841 ttcctggcgt ttgtacagtt ctgctaaatc tgcacggaca gtttctgtag cggcatcttt
      901 gtcttgaatg gatgggtctg cataaagctg tgagagcaaa tattcttttt caagaataaa
      961 ttgggtcgtt ttgaaatatt ccattacgac ctgtttttgc tcttcgcggt tgagtgtttg
     1021 cggcaggttt atggaagcgg cgcgcatttt tagcacggcg gcatttgcca tccattccac
     1081 ataatcaaat tcaaggctgc gcgtatatgc gcggaccttt tcgatcggat tggtgaggga
     1141 ggggttgctg tagccagtaa gcgcggcaag gacaaagagc aggatcgtga tctcgagccc
     1201 gcgtaaaatt cgttgagcca tagggtggat tatacccagt tcgcttatct ctgttcttcg
     1261 gcgattcttt tgaacacctc aagggtcaca ttccagtaaa acgccatatg tttataaatg
     1321 gactctgttg gaaagttcct cagggtgaac attaattgtg ttttttggtg gatagagtgt
     1381 agttggaagt ctagaattga gtagttttct gcccggtctg gaagatggga tactgaactg
     1441 aggtgactat attgaagatt tttctcaggc tcaaatttta ggacaagccc tttattttcg
     1501 aactcttttc cgctcattgt gccgcgaatg aaaatagggc tgccgacttc ccaatcggtt
     1561 tggatggaga gctcaatatc tggcatcatc cattttttca tctgttcggg ctcggtcagc
     1621 agcatccaca ctttggatgt ggttgctgca atttcaacag acttttgaaa gatggttgag
     1681 ttattaagtg atgtgttcga tgtcattttt tgttatcgcc aatcatcggc tatgaatgct
     1741 acatctgcca ggttgaaaac atctgaggat ggaaactctt ccaaatttac tttgtctagc
     1801 cgggatttgt tgaagccaag atttaatact tcttgttctg tggaattatt attcgctata
     1861 gagggtggaa acattcctat caagataaca taaatatagc gggtcttatt ctttttgagc
     1921 gtttttagga tgtttacagc atcaatttgt gcattgcttt ctgttaatgc tggagtcgaa
     1981 tacctttgaa gagaccatgt aatggtaatg tcaccatttt ccggggtgct atacgcgatc
     2041 tttgttaagc gaggcgtgtt tttcctgtcc acgcctagta tttgtgacaa gtcttcttgt
     2101 aagacagttt tctcgtccgt gatggctgat tgcgtcggcg atggggcaat ttcggtcttt
     2161 gtggtttgtg ccctg
//
LOCUS       DAOQNJ010000490         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_41399_length_2175_cov_6.370283, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000490 DAOQNJ010000000
VERSION     DAOQNJ010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41399_length_2175_cov_6.370283"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLG52_12040"
     CDS             <1..197
                     /locus_tag="PLG52_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42224.1"
                     /translation="AFVVKFPTYSTVSTACVTCAGVWVNPLGGRKRPTAEKILENAAE
                     SPASSARFVSPLFADQQPSL"
     gene            complement(160..840)
                     /locus_tag="PLG52_12045"
     CDS             complement(160..840)
                     /locus_tag="PLG52_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42225.1"
                     /translation="MNHIYIPTSSADDWRKFLAEPDKQWRSGYSAKELAECWENANGF
                     PVELQSMFAKSTNQALSDLELLLAIPEYQVNLPDGKRPSQNDLFVLARAKDGQLVSIM
                     VEGKVSEPFGETLEVWLKDASEGKKKRLKILCDILGLQNEPPLNIRYQLFHRTSSAIL
                     EAKRFNAKYAVMLVHSFSPEHKWFSDYQDFLGLFGVSSKINELVELPESEGKRIFTGW
                     VVGQQKAG"
     gene            complement(1019..>2175)
                     /locus_tag="PLG52_12050"
     CDS             complement(1019..>2175)
                     /locus_tag="PLG52_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB42226.1"
                     /translation="LAMYIAVAKRQGADMRELRGTIQNDILKEYVARGTYIFPPAPSM
                     RLITDIFQFCSKEVPYWNTISISGYHIREAGSTSVQEVAFTLANGIAYVEAALAAGLN
                     VDDFAGQLSFFFNAHNNLLEEVAKFRAARRMWARIMRERFKAQKPSSWQLRFHTQTAG
                     STLTAQQPENNVVRVTLQALSAVFGGTQSLHTNSMDEALWLPTEKSVRVALRTQQIIA
                     YESGAADSVDPLAGSYLIEHLTDEIEKGALEYIAKIDEMGGAMQAIERGFMQNEIQNA
                     AYAAQQAIEKKEEIVVGVNAFEVKEERTLERLSVDPAIEVGQREELRKLREARDNEKV
                     NWYIGRLVEAAKGTENLMPLFIECVEHDLTLGEICNTLRGVWGEYVAEGF"
BASE COUNT          530 a          593 c          470 g          582 t
ORIGIN      
        1 ctgcattcgt tgttaaattc cccacttatt cgacagtctc aacggcttgc gttacttgcg
       61 ctggggtttg ggtgaatccg cttggtgggc gaaaaagacc gacagcggag aaaatactcg
      121 aaaatgccgc agaatcccca gcgtcaagtg cacgctttgt tagcccgctt tttgctgacc
      181 aacaacccag cctgtaaaga tgcgcttgcc ttcggactct ggcaactcta ccaactcgtt
      241 gattttactt gaaacgccaa ataaacccaa aaaatcttga taatctgaaa accatttatg
      301 ttctggacta aaagaatgaa caagcattac cgcatattta gcattgaatc tttttgcttc
      361 aagaattgcc gatgatgttc tatgaaataa ctgatagcga atattcaaag gtggctcatt
      421 ttgtaagcct aaaatatcac acaatatttt taggcgtttc ttttttcctt cggaagcgtc
      481 ttttagccat acttctaaag tttcgccaaa cggctctgat acttttcctt ccaccattat
      541 tgaaaccaat tgaccatctt ttgcacgagc caaaacgaat aaatcatttt gagatgggcg
      601 ttttccatct ggtaaattta cttgatattc aggaatagcc aataataact ctaagtcgct
      661 taatgcctga ttagttgact ttgcgaacat gctttgaagt tctacgggga agccattagc
      721 attttcccaa cattctgcca attctttagc cgaatatcct gaacgccatt gtttatcggg
      781 ttctgcgaga aactttcgcc aatcgtctgc tgatgatgtt ggaatgtaaa tatgattcat
      841 tttagatttc ccaataattt tgcgcgggcg ggctaactat atattagaca gaaaggtagc
      901 tacatctagt aacccctgat ttaagggtga ctacatctag taaccgggtg actgcatcta
      961 gtaatcactg cgcaccttcc atacccgcca ttataagccc gttgatgcgg tggggggact
     1021 aaaacccctc cgccacatac tctccccaaa ccccgcgtag cgtattacag atctcgccca
     1081 gggtcaggtc gtgctccaca cattcaatga acagcggcat caggttctcc gtccctttgg
     1141 cggcttccac caatctacca atataccaat ttaccttctc gttatccctc gcctctctca
     1201 acttccgcaa ctcctcacgc tgacccactt caatggcggg gtctaccgag agcctttcga
     1261 gcgttctttc ttccttcacc tcaaaggcat ttacgcccac cacgatctct tctttctttt
     1321 caatcgcttg ctgcgccgca taggccgcgt tctgaatttc gttctgcatg aatccgcgct
     1381 caatggcttg catcgccccg cccatttcgt cgatcttggc gatgtactcc agcgcgcctt
     1441 tttcaatttc atcggtcaga tgctcgatca gatacgaacc cgccaacggg tcaaccgagt
     1501 cggctgcacc cgattcatag gcaatgatct gctgtgttcg cagcgccaca cgcaccgatt
     1561 tttcggtggg cagccacaag gcttcgtcca tgctgttggt atgcaggctt tgcgttccgc
     1621 cgaagacggc ggaaagagcc tgtaacgtaa cacgcaccac attattttcg ggctgctgcg
     1681 cggtcagggt cgaaccggcg gtctgggtgt ggaagcgtaa ctgccacgaa gaaggcttct
     1741 gcgccttaaa gcgttcacgc ataatgcgcg cccacatgcg ccgcgccgca cggaatttgg
     1801 caacctcttc gagtagatta ttatgcgcgt taaagaagaa cgagagttgc cccgcgaaat
     1861 catccacatt gagtcctgcc gcaagcgccg cctccacata ggcgatcccg tttgcgagcg
     1921 tgaaagccac ttcctgcacc gaggtcgaac cggcttcacg aatatgatag cccgagatcg
     1981 agatcgtatt ccaatatggc acttccttcg aacagaactg gaagatgtcg gtgatcaggc
     2041 gcatcgacgg agcaggcgga aagatatacg tcccacgcgc cacatattcc ttgagaatat
     2101 cgttctggat cgtcccgcgc agttcacgca tatctgcgcc ctggcgtttg gcaaccgcaa
     2161 tatacattgc cagca
//
LOCUS       DAOQNJ010000491         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_41853_length_2162_cov_6.014238, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000491 DAOQNJ010000000
VERSION     DAOQNJ010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41853_length_2162_cov_6.014238"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1673
                     /locus_tag="PLG52_12055"
     CDS             48..1673
                     /locus_tag="PLG52_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNB42227.1"
                     /translation="MLSIHNLQKNFGIQPVLKNINFNISAGERIGLIGANGSGKTTLM
                     RILAGFEHPDSGDVASTHPNLRVGYLAQGMEFEPQETLQSALRLDSDSQSDPALEVES
                     LALALSQNPNDTALQAQYDSALLKLSGDSTQPSAVLEPLGLSHFPVDTPIAHLSGGQK
                     TRLMLARVLLGDPRLLLLDEPTNHLDIEMLEWLENWLNHFNGAALIVSHDRTFLDNTV
                     TKVLELDSFTHTIKTYDGNYSDYLEQKLLEYDKQMQAYQDQQDEIAQLKRAARHIRNL
                     TVMKKGGKSDSGDKFAKGFFGNRATKNVAGRAKNLEARIDQLLTDDKIDKPQGTWKLK
                     LDFGAPEHQSKDVLVTDSLSVGYTLEQPLLTNINLFIRAGQRVVLTGANGSGKTTFIR
                     TIVGKIPALTGSFRLGGATKLGYMAQEQELLNPALNSVQTIQSVSAINETETRNFLHY
                     FLFKGDAALRPAGELSFGERARLQLALLVAKGCTFLILDEPINHLDIPSRERFEEALE
                     NFNGTILAVVHDRSFIERFASDVWQAKDGKITK"
     gene            1766..>2162
                     /locus_tag="PLG52_12060"
     CDS             1766..>2162
                     /locus_tag="PLG52_12060"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNB42228.1"
                     /translation="MKFETLAIHAGQEPDPSNGAVMTPVYFTSTYKQDGIGKPRQGYE
                     YSRTLNPTRKALQDCLAELESGQWGLAFASGMAATDTVLRMLAQGDHVVAGNDVYGGT
                     FRLFDKILRRFGLDFTFADTTDPENLAEAL"
BASE COUNT          565 a          539 c          536 g          522 t
ORIGIN      
        1 tctgccgagg cttgccctcg gttttctttt taactacagg tgtcattatg cttagtattc
       61 acaatcttca aaaaaatttc ggcatccagc ccgttctcaa aaacatcaac ttcaatatca
      121 gcgcaggtga acgcatcggt ctgatcggcg cgaatggctc cggcaagacc acgctcatgc
      181 gtattctcgc cgggttcgaa catcctgatt cgggggatgt cgcttcgacg cacccgaatc
      241 tacgggtggg ctaccttgcc cagggaatgg aatttgaacc gcaggaaacg cttcaatccg
      301 cgctcaggct tgattcagat tcccaaagcg accctgcgct ggaagtggag tcccttgccc
      361 tggcattatc tcaaaatccg aacgatactg cactgcaagc acaatatgat tcagcgttgc
      421 ttaaactctc cggagatagc acccagccgt cggcggtgtt ggagccgttg ggcttgtccc
      481 acttccctgt tgatacaccg atcgctcatc tctctggtgg tcaaaaaaca cgtctcatgc
      541 tcgccagggt tttgttagga gatccacgtc ttctattatt ggatgagccc accaaccatc
      601 tcgatattga aatgctggag tggctggaaa actggctgaa tcactttaat ggcgccgcac
      661 tgatcgtctc gcatgaccgc acatttttgg ataataccgt cacgaaggtg ctggagttgg
      721 attcatttac acacacgatc aaaacatacg acggcaatta cagcgattac ctcgagcaaa
      781 aattgcttga atacgacaag caaatgcaag cctatcagga ccaacaggat gagatcgcac
      841 aattgaagcg tgcggcaaga cacatccgca atttgacggt gatgaaaaaa ggcggcaagt
      901 cggatagcgg cgataaattt gcaaaaggct tcttcggtaa tcgcgcaaca aaaaatgtag
      961 cagggcgcgc caagaacctc gaggctcgca ttgatcaact acttacggat gataagatcg
     1021 ataaaccaca aggcacatgg aaattgaaat tagatttcgg cgcgcctgaa catcaatcta
     1081 aagatgtgct tgtaacggat tcgctctctg ttggctacac gctagagcag cctttattga
     1141 cgaatatcaa tctgtttatc cgtgccgggc aacgtgtggt gttaacggga gcgaacggct
     1201 ctggtaagac aactttcatc cgaaccattg ttggaaaaat tcctgcattg acgggttcct
     1261 tccgcctcgg cggcgcgacc aaattagggt atatggccca ggaacaggaa ttgctcaacc
     1321 cggcgctgaa ttcggtgcag accatccaaa gtgtttctgc gattaacgaa acagagacgc
     1381 gtaactttct gcattatttc ctgttcaaag gagatgccgc cctgcgcccg gctggtgagc
     1441 tatccttcgg tgaacgtgcc cgtttgcaat tagctttgct tgttgccaaa ggttgtacgt
     1501 tcctgatcct ggatgaaccg attaaccatt tggatattcc ttcgcgggag cggtttgaag
     1561 aagcgctgga aaatttcaat ggaacaatcc ttgccgttgt ccatgaccgt agttttatcg
     1621 aacgcttcgc cagcgatgtt tggcaggcaa aagatggaaa gataaccaaa taacagagta
     1681 tataatcgag ggaaagcttt taccattccg agattgcacc ggcgaagatc atcgccttgc
     1741 aataccattt accaaaggag catgaatgaa atttgaaact ctcgccatcc acgcagggca
     1801 ggaacctgac ccaagtaacg gcgccgtaat gacgccggtc tatttcacat ccacatacaa
     1861 gcaggacggc attggcaagc cgcggcaagg atatgaatat tcgcgcacgt tgaacccgac
     1921 gcgtaaggcg ttgcaagatt gtttagcgga attggaaagc ggccagtggg gattggcttt
     1981 tgcttcagga atggcggcaa cggatacggt tttgagaatg cttgcgcaag gcgaccatgt
     2041 tgtggctggt aacgatgtct acggcggtac atttcgcctc tttgacaaaa tcctgcgccg
     2101 cttcggcctg gactttacct ttgccgacac aaccgatcct gagaatctcg ccgaagcctt
     2161 aa
//
LOCUS       DAOQNJ010000492         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_42129_length_2154_cov_8.664126, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000492 DAOQNJ010000000
VERSION     DAOQNJ010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42129_length_2154_cov_8.664126"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLG52_12065"
     CDS             complement(<1..848)
                     /locus_tag="PLG52_12065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB42229.1"
                     /translation="MRVTFIRPHITARRAADALEPLVFGILAKLTPPDVEVKFYDARI
                     EPVPLDEPTDLVALTVETFTAKYAYQLAMHYRARGVPVVMGGYHPTLLPDEALQFADA
                     IAIGDAETVWAQIVEDARAGRLKRVYQSQPNLETDGVVPDRSIFAGKKYKPLHLMQIG
                     RGCKYACDFCSIHAFYGRDMPRRCLDDVLKEAEELRGKYVFFADDNLFNDLEYAEKLF
                     KALIPYNIHWSCQVSLDIAQNPHLLDLMAQSGCIGCLIGFETLDEANLVQMRKKWNKK
                     YGDYANT"
     gene            complement(873..2024)
                     /locus_tag="PLG52_12070"
     CDS             complement(873..2024)
                     /locus_tag="PLG52_12070"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB42230.1"
                     /translation="MQKVFLSPILETLADGLVWLCMNLERLPLPFLVSRTGYANPRVR
                     RITGWAGYWAHTFRLTLPNIHTWMKNKGDLFPLVVQVQTINRCNASCSFCPYPYTVHL
                     QEKRIMDDELYSKIVDECVSEPGLLDFVPMSKNEPLLDVNMEERIREFRSKAQPHQIV
                     ELVTNGSPLTSARAKRLMDAGLDMITVSVSASNEETYKKVMVGLSWKQVMNNLEALAQ
                     ENLAGLNVYLRFIKERGNRRELSAFRKRWRHFNHFNFNVNNRAGALRGYEMKSIQYGS
                     FAERLKHMLGSHIYPACPYVFSSMHILENGDVTMCLNDWGDREVIGNVRAQSIRDIYN
                     SPRMKEVRELMVQGRYEEIEACKGCSFYHEWLKPLTAQEKVQVAEPVIG"
BASE COUNT          540 a          593 c          497 g          524 t
ORIGIN      
        1 gtgttggcat agtcgccgta tttcttgttc cacttcttac gcatctgcac caggttggct
       61 tcgtccagcg tttcgaaccc gatcaaacag ccaatgcagc cgctctgcgc catcaaatcc
      121 aacaggtgcg ggttttgagc aatatccaaa ctgacctgac aagaccagtg aatgttatac
      181 ggaatcagcg ccttgaataa tttttcggca tactcaagat cgttgaataa attatcatcg
      241 gcaaagaaaa catacttgcc gcgcaattct tcggcttcct tcaagacatc gtccaaacaa
      301 cgacgcggca tatcgcgccc ataaaaggca tggatcgaac aaaagtcgca ggcatattta
      361 catccgcgcc cgatctgcat cagatgcaac ggcttgtatt ttttacccgc aaaaatactg
      421 cggtctggca ccacgccatc cgtctcaaga tttggctgcg attgatacac gcgtttcaac
      481 ctgcccgccc gcgcatcttc cacgatctgc gcccaaaccg tctccgcatc accaatcgca
      541 atcgcatcgg caaattgcag ggcttcatcg ggcaacaatg tggggtgata cccgcccatc
      601 accacgggta caccccgcgc gcgataatgc attgcaagtt ggtaagcata tttggcggtg
      661 aacgtttcca ctgtcaacgc tacaagatcg gtcggctcat caagcggcac aggttcaata
      721 cgcgcatcat aaaatttgac ctccacatca ggcggggtca actttgccaa aatgccaaag
      781 accaacggct cgagcgcatc tgccgcgcgg cgtgctgtga tgtgtggacg gataaacgta
      841 actctcatgg gataaagtgc ctcgcttctt tcttaaccga taacgggttc agctacttga
      901 actttttctt gcgctgtgag cggtttcagc cactcatggt agaacgaaca tcccttgcaa
      961 gcctctatct cttcgtacct tccctgcacc atcaactcgc ggacttcctt catacgcggt
     1021 gagttgtaaa tatcgcgaat gctttgcgcg cggacgttgc cgatcacctc acggtcgccc
     1081 caatcattca aacacatggt cacatcccca ttttccaaaa tatgcatgga actgaacaca
     1141 tacgggcagg cagggtagat atggctcccc agcatatgct taaggcgttc ggcaaaactc
     1201 ccatattgaa tggacttcat ttcatatcca cgcagcgctc cagcacgatt atttacattg
     1261 aaattgaagt gattaaaatg ccgccagcgt ttgcgaaagg cactcaactc cctgcgattg
     1321 ccccgttcct tgataaatcg taaatagaca ttcaacccag ccaggttttc ctgggcaagc
     1381 gcttcaaggt tattcattac ctgcttccac gaaagcccca ccatcacctt tttataggtc
     1441 tcttcattcg aagcgctgac actcacagtg atcatatcca acccagcatc catcaggcgt
     1501 ttggcacgcg cggaggtcag cgggctgcca ttggtgacaa gctccacgat ctggtgaggc
     1561 tgcgctttcg atctgaattc gcggatgcgt tcttccatgt tgacatctaa caacggctcg
     1621 ttcttggaca tggggacaaa atccaacagc cctggctcac tgacacattc atccacgatc
     1681 ttggagtaca gttcatcatc catgatgcgc ttttcctgca aatggactgt gtatgggtaa
     1741 gggcaaaaac tgcatgaggc attgcagcgg ttaatggtct gtacctgcac cactagcgga
     1801 aagaggtcac ctttgttctt catccatgtg tggatgttag gcagggttaa gcgaaaggtg
     1861 tgagcccaat accccgccca ccccgtaatg cggcgtacac gcgggttggc gtatcccgta
     1921 cgtgagacga ggaatggcag aggcaatctt tctaaattca tacatagcca cacaagacca
     1981 tctgcaagtg tttcaagaat aggcgataaa aaaacttttt gcatgttcac ctctaaagca
     2041 aagcaatctc aggcgtggag ggacgggcat ccacgcgggc gatttcgtcg aagccatcat
     2101 cccaggcggc gaggtaaatg tcgctttcgt atgtgagggg tcggtaggtt ttga
//
LOCUS       DAOQNJ010000493         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_42130_length_2154_cov_8.154359, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000493 DAOQNJ010000000
VERSION     DAOQNJ010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42130_length_2154_cov_8.154359"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PLG52_12075"
     CDS             <1..1236
                     /locus_tag="PLG52_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNB42231.1"
                     /translation="VLAGNGLKVYLTDGATPTPVIAYAVVDKKAAGAVNITASHNPPT
                     DNGFKVRNETGGAIDPEGLKQIEAGIPDDLKDVKLKNYKEAEAAGEIVKFDAATPYIE
                     HLTRDGLIDLQPIKDAGLTVVVDAMWGNGAGWFTRLLGGGKTKIIEIHNERNPSFPEM
                     KRPEPIRPNIDVGLKATVTNKADVLLITDGDADRCGLGDENGEFINQLRAYALLAYYL
                     LEVRGERGDIVKTLSTTGMLNKLGEIYGVPVHETGVGFKYVAPKMTETNALIGGEESG
                     GYAFRGNVPERDGILAGLYMLDFMVKTGKKPTQLLKDLFAKVGGEYYYDRVDSPFSGD
                     HETRKQIIRDNYPATLGGLKVTEVVTVDGFQFKLEDGGWMLIRFSGTEPILRVYTETR
                     HADKVQAILQDGLKVAGIK"
     gene            1534..2073
                     /locus_tag="PLG52_12080"
     CDS             1534..2073
                     /locus_tag="PLG52_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42232.1"
                     /translation="MPGILILYQQWWSGVFNVAFYDLGISLRTRHGVTDIQWRDIDSM
                     WLEWAQSGTQSSPRTTLAAYAFRLKSGELFRLRSNMEGFNEFEEKFYKPASAAILQTQ
                     MDDLQMGKKVSIYGIGIDPSGLSYGLKSILWTDIEALKAEKDKLLIKQNGEWNVWETF
                     AFHVPEEVLMLSKVFTKVE"
BASE COUNT          572 a          489 c          549 g          544 t
ORIGIN      
        1 gtgttggctg gcaacggctt gaaggtctat ctaaccgatg gcgcgacccc aaccccggtc
       61 attgcttatg ccgtggtgga taaaaaagct gccggtgcgg tgaacatcac agccagccac
      121 aacccaccca ccgataatgg cttcaaggtc cgcaatgaga ccggtggcgc tattgaccct
      181 gaaggtttga aacaaatcga agcaggcatt cctgatgatt tgaaggatgt aaagctcaaa
      241 aattataaag aagccgaagc cgctggcgag atcgtcaagt tcgatgcggc gactccgtat
      301 attgaacacc tcactcgcga tggattgatc gatttacagc ccatcaaaga tgccggtctg
      361 accgtggtgg tggatgccat gtggggcaat ggtgcaggtt ggttcacccg tctgctcggc
      421 ggcggtaaga ccaagatcat tgaaattcac aatgaacgca acccttcctt ccctgaaatg
      481 aagcgcccgg aacccatccg cccgaacatt gatgtaggtt tgaaagcgac tgtgaccaac
      541 aaagccgatg tgttgctcat caccgatggt gacgcagacc gctgtggctt gggggacgag
      601 aacggtgaat tcatcaacca attacgcgcc tatgcgttgc tggcgtatta cttgctcgaa
      661 gtacgcggtg aacgcggcga catcgtcaag acgctttcaa caactggtat gttgaacaaa
      721 ctgggcgaga tatacggcgt acctgtccat gagacggggg tgggcttcaa gtatgttgcc
      781 ccgaagatga ccgagaccaa tgcactcatc ggcggtgaag aaagcggcgg ctatgccttc
      841 cgtgggaacg tgcccgaacg cgatggcatt ttagccggct tgtatatgct cgatttcatg
      901 gtcaagaccg gcaagaaacc gactcaattg ctcaaggact tgttcgctaa agtgggtggt
      961 gagtattact acgaccgggt tgatagtccc ttcagcggtg accatgagac tcgcaagcag
     1021 attatccgcg ataactaccc tgccacatta ggcggactga aagtgaccga agttgttacg
     1081 gtggacggct tccaattcaa gcttgaagat ggcggctgga tgttaatccg cttctcgggg
     1141 acggagccga tcctgcgcgt atataccgaa acaagacatg ctgacaaagt gcaagcgatt
     1201 ttgcaagacg ggttgaaggt tgctgggatt aagtaagtaa agtgtgttca aagtgtcagg
     1261 tcttttatga cctgacactt ttttattaag caaaaggagt ttatcggtat gtcaggtcat
     1321 tttccacctt ttggcgcgtt aggcaacttg aaaagtgagc acgctttatc aaagcgatta
     1381 caaagaacca tgctgatctt tgggggaatt tggttatgcc tactatgtat agatatcggc
     1441 atttcaattt ctgttttcaa cacaagttat gaagagtcta ataacgctct cacggctctt
     1501 atcgttccaa taatatgtgt tggcattttt gctttgcctg gaatattgat tctctatcaa
     1561 cagtggtggt caggtgtgtt caatgtcgcg ttttacgatc taggaatttc tctccgtacc
     1621 cggcatggag ttacagacat tcagtggcgc gatattgact ccatgtggct ggagtgggcg
     1681 cagtctggga cgcaatcttc cccaagaaca acccttgccg cctatgcctt ccgcctcaaa
     1741 agcggagaat tattccgtct acgcagcaat atggaagggt tcaacgagtt tgaagaaaag
     1801 ttctacaagc ctgcttctgc tgccatttta caaactcaaa tggacgatct acagatgggc
     1861 aaaaaagttt ccatttatgg gattggaatt gacccatctg gtttgagcta tggactcaag
     1921 tctattctgt ggacagatat cgaagccctt aaagcagaga aagacaaatt gcttatcaag
     1981 caaaatggcg aatggaacgt atgggaaacc ttcgccttcc atgttccaga agaagtgcta
     2041 atgctctcaa aggtatttac gaaagtagaa taacagaaaa gacactccat atggagtgtc
     2101 ttttctgttg gctaacgttt acgttttaca ctcaatacca acccaaataa tgcc
//
LOCUS       DAOQNJ010000494         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_42132_length_2154_cov_6.080515, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000494 DAOQNJ010000000
VERSION     DAOQNJ010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42132_length_2154_cov_6.080515"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLG52_12085"
     CDS             <1..796
                     /locus_tag="PLG52_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB42233.1"
                     /translation="SNMAIMLRGNVIRIPAPAENGFIPQFDSWLKALTPKTRIIVLNY
                     PSNPTGAFPSREYLQKLQDFAKEHHLWIVSDEVYGSLYYEGKPTCAADFEGAKERTLL
                     VNSLSKSYAMTGWRVGFLAAPARVIQNALKAGQNSITCVAPFIQKAATFALIEPKIQD
                     VTVEMRAAYSRRRDLVIRLSHELESDVVKIIPPQGAFYFFLDLRALKMPSVEMCEKIL
                     DEEGVGLVPGSAFGEQGEGFIRMTIAASEEDVEAGFRKIVAWAERQ"
     gene            835..1899
                     /gene="cax"
                     /locus_tag="PLG52_12090"
     CDS             835..1899
                     /gene="cax"
                     /locus_tag="PLG52_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HNB42234.1"
                     /translation="MPTFLKNEWTTFVIALIASALTGILLSSNANAVLTFTIGAIALA
                     TLATVVGHATEQLGNYLGPGATGVLQSAIGNLPELFVAFFALKAGLVTVVQSALVGSI
                     LANSLLVLGLAFLFGGIKNGTQKFHSETPRAIVMLMVLAVSALVFPTLAHSLHTPAEA
                     HEVPLSVASAVILLIVFFASIPLSLQGGPIADVAPTEHKEKPWPIWRILTILAIAGSG
                     AAFVSDWFVEALEPAIEMLGISQAFAGLVVVAIAGNAVENVVGIQLAIKNKSDYALSV
                     ILNSSLQIALALTPILVLLSFFFTSTVLTLVLPPLLVVALTLTAILSAFIVYDGESNW
                     LEGVALVGLYAIIAMSFWWG"
     gene            1902..>2154
                     /locus_tag="PLG52_12095"
     CDS             1902..>2154
                     /locus_tag="PLG52_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNB42235.1"
                     /translation="MRVSFQGEPGAYSEQAVFNYYGKDVETVPCESFDVMFDSVISGK
                     SDAALAPIENSLAGSIHQNYDLLLRHNLHIVGEYFLRVRH"
BASE COUNT          490 a          523 c          536 g          605 t
ORIGIN      
        1 ttccaatatg gccatcatgc tgcgcgggaa tgtcatccgc atcccggccc ccgccgaaaa
       61 tgggttcatc cctcaattcg attcgtggct gaaagcgctg actcccaaaa cgcggatcat
      121 cgttctgaac tatccctcca acccaaccgg cgcattccct tcgcgagaat atttgcagaa
      181 gttgcaagat tttgcgaagg aacatcatct ctggatcgtc agcgacgagg tctatggaag
      241 tttatactac gaaggcaaac ccacctgtgc cgcagacttt gaaggcgcca aagaaaggac
      301 gttgctcgtc aacagcctgt ccaagtccta tgccatgacc ggctggcggg ttggctttct
      361 ggctgcgcct gcccgtgtga tccaaaacgc tctgaaagcc ggacaaaact ccattacctg
      421 tgtagctcca ttcattcaaa aagccgcgac ctttgcactg atagagccaa aaatacagga
      481 tgtcactgtt gaaatgcgcg ctgcttatag ccgccgtcgc gacctggtga ttcgcctctc
      541 acatgaactt gaaagtgatg tggtcaaaat catacctccg cagggcgcgt tctacttttt
      601 ccttgacttg cgcgccttga aaatgccctc cgtggaaatg tgcgaaaaaa ttctcgatga
      661 agaaggcgtg ggccttgtgc caggctctgc atttggcgag cagggagaag gtttcatccg
      721 aatgaccatt gccgcttcag aagaggatgt ggaagcgggt tttcgaaaaa ttgttgcctg
      781 ggctgaacga caataaacta caatacgatt caaatagatt taggagatct taaaatgccc
      841 acctttctaa agaatgaatg gacgaccttc gtcatcgcat taatcgccag cgcattaaca
      901 ggcatattgc tatcctcaaa cgccaacgcc gtcctgacct ttacaatcgg tgcaatagcc
      961 ctggctactc tcgcaacggt cgttggtcat gctactgaac agttgggtaa ttatctggga
     1021 ccgggtgcaa ccggcgtact gcaatcggcc ataggaaatc tgcccgaatt atttgtcgcc
     1081 ttctttgctt taaaggccgg gttggtcact gtggtgcaat ctgcgctggt agggtccatt
     1141 ttggcgaaca gcctgctggt gctggggttg gcctttttat ttggcggaat caaaaatgga
     1201 acccagaaat tccactctga aaccccgcgc gccatcgtca tgttgatggt gctggccgta
     1261 tccgcccttg tttttccgac tctggctcat tccttgcaca ctcctgctga ggctcatgag
     1321 gttccattaa gcgttgccag tgccgttatt ttgctaatcg ttttctttgc aagtattcca
     1381 ctatctttgc aaggtggccc tattgctgat gttgctccta ccgagcataa ggaaaaacca
     1441 tggcccattt ggcggatatt aaccatcctg gctattgcag gcagcggtgc agcttttgtt
     1501 tctgattggt ttgtagaggc acttgaacct gccattgaaa tgctaggcat ctcgcaggca
     1561 tttgcgggtc tggttgtggt ggcaattgcc ggaaatgctg ttgagaacgt tgttggaatt
     1621 caacttgcca ttaaaaacaa gtcagactat gcattaagtg ttatcttaaa tagctcgctt
     1681 cagattgcac tggcattaac cccgatcctg gtattgttgt cgtttttctt tacttctaca
     1741 gtcttaaccc ttgtgttgcc gcccttgttg gttgttgccc ttacactaac agcaattttg
     1801 agtgcgttca ttgtttatga cggcgagtca aattggcttg aaggcgtggc attggtcggt
     1861 ctgtatgcca taatcgctat gtcattttgg tgggggtagg aatgcgagtc tcttttcaag
     1921 gtgaacccgg cgcgtatagt gagcaagcgg tctttaatta ttacggcaaa gatgttgaaa
     1981 cggttccatg tgaatcgttt gatgtgatgt tcgattctgt catctctggc aagagtgatg
     2041 cggcgttggc tcccattgag aattccttgg cgggcagcat tcaccagaat tacgaccttc
     2101 tcttaagaca taacctgcat attgtggggg agtatttctt gcgcgtgcgg catt
//
LOCUS       DAOQNJ010000495         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_42174_length_2153_cov_10.675405, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000495 DAOQNJ010000000
VERSION     DAOQNJ010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42174_length_2153_cov_10.675405"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLG52_12100"
     CDS             complement(<1..374)
                     /locus_tag="PLG52_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNB42236.1"
                     /translation="MKPEGKRYSTVVGNKTVTIETGRLAGQAGGALTLGIDEAIVFAS
                     ATMGGVRDGIDFFPLSVEYEERLYAGGKIPGSFFRREGRASTESILTARLTDRPLRPL
                     FQDGMRNEVQVIMYSFSSDGVNP"
     gene            complement(554..826)
                     /gene="rpsO"
                     /locus_tag="PLG52_12105"
     CDS             complement(554..826)
                     /gene="rpsO"
                     /locus_tag="PLG52_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNB42237.1"
                     /translation="MPLAKEVKTKAIEDFHRHEKDTGSPEVQVAILTNRITQLTEHLR
                     NNKQDQSCRRGLLKLVGQRRRLLTYLRQSNYQSYLSVTERLNLRRK"
     gene            complement(913..1416)
                     /locus_tag="PLG52_12110"
     CDS             complement(913..1416)
                     /locus_tag="PLG52_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB42238.1"
                     /translation="MDKKEIEFQLEDTQRRFIELVNSIPEENYSLPTDNPAWSVGDIL
                     FHITLGPRALALEIWMTAHASGFFGFMMRHFPSRLFNAINAWFGRGRSQRVSRQSLLK
                     AYGKAHAVIMSELRRTREEDLVKSVVYPPEFVSDLAGEVTPERLFRYVTGHFEAHEGQ
                     LRRDSLV"
     gene            1675..>2153
                     /locus_tag="PLG52_12115"
     CDS             1675..>2153
                     /locus_tag="PLG52_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB42239.1"
                     /translation="MSSVISISKLAKHYQVPEREAGLKAAAKGLFKRTYKTVKAVDGI
                     SFDIQPGEVVGFLGPNGAGKTTTLKMLSGLLHPTSGSLSVLGYEPAKRNHDYLRQITL
                     VMGNRNQLSWDLPALDSFDLQRAIYGIPTDEFKRTRDEFIELLDLKELVQKPVRNLS"
BASE COUNT          517 a          541 c          532 g          563 t
ORIGIN      
        1 gggttcacgc cgtcagacga gaacgagtac atgatgactt gcacttcatt gcgcatgcca
       61 tcctgaaaga gtgggcgtag aggtcggtca gtgaggcgag cggtgaggat cgactcggta
      121 gaggcgcgcc cttctcggcg gaagaaagag ccgggaattt ttcctccagc atacaatctt
      181 tcttcgtatt ccacgctcaa ggggaagaag tcgattccgt cgcgcacgcc gcccatggtg
      241 gctgaggcga acacaatggc ttcgtcgatt ccaagcgtaa gagcgccgcc agcctgcccg
      301 gctaagcgac ccgtttcgat ggttaccgtt ttgttaccaa cgacggttga gtaacgttta
      361 ccttcgggtt tcatatttaa ttgtctccta aaatcttttg ggcgagtcac ggtcgcccct
      421 gaaagctccc gcctcggggt cagggactga agagtgggaa caacagagtt attcgcgcta
      481 ttcaattcct gacggcgggt ttacacaatg taggggcaca ggttcactgt gcccctacgg
      541 ggtttcgttc tatttacttg cggcgcaagt tcaaacgttc agtgacactg aggtacgact
      601 gatagttgct ctggcgcagg taggtgagta agcggcggcg ctgacccacc aacttgagca
      661 aaccacggcg gcaggactga tcctgcttgt tgttgcgcaa gtgttcggtc aactgagtaa
      721 tgcggttggt caaaattgcg acctgaactt caggtgagcc ggtgtctttc tcgtggcggt
      781 gaaaatcttc aatcgccttt gtcttgactt cttttgcgag gggcatgacg tatgcgtctc
      841 ctttctaata atattgcagg tctccgtgcc gaaagctttt aaaccgtcgg taggaccagt
      901 cacggggatg gattatacca gagaatcccg ccgtaattgc ccctcatgcg cctcgaaatg
      961 ccctgtcaca taccgaaaaa gacgctctgg cgtgacctct ccagccaggt ctgacacaaa
     1021 ctcaggcgga tacaccaccg atttgactaa atcctcctcc cgcgttcgcc tcaattcaga
     1081 cattatgact gcatgtgcct tcccataagc cttgagcaaa ctctgacggc tgactcgttg
     1141 gctccgtccg cgcccgaacc aggcattgat cgcgttaaag agccgtgaag ggaaatgccg
     1201 catcataaag ccaaagaatc ctgaggcgtg tgctgtcatc caaatttcca gcgccaatgc
     1261 ccgcggaccc aaggtaatgt gaaagagaat atctcccacg ctccaggctg ggttgtctgt
     1321 agggagagaa tagttttctt caggaatcga attaaccaat tcaataaatc gacgttgcgt
     1381 atcttctaat tgaaattcaa tctctttttt atccattttg ctttccatct tgcatttgat
     1441 ttttggctgt gatacacttt gcttatctaa ttctaatctg aaaggaggta cttcttgaat
     1501 gactatcatt aatcatgaaa caacaaatct atgcgtgcct ccccgcgggt gggttgggta
     1561 attcattccc attttgaaac cttgccgcag gcgcgctgat gccctgcggt tttttattgc
     1621 catagggcgg actattgata ccgaacagaa agctaccata ttcaggagaa atctatgtcg
     1681 tctgtcattt cgatctcgaa actggcaaaa cattaccaag tccccgaacg tgaagcgggt
     1741 ttaaaagccg ccgcgaaagg gctcttcaaa cgcacctaca agaccgtcaa agctgtagat
     1801 ggcatctctt ttgatattca acccggagaa gtggtcggtt ttctcggtcc gaatggggca
     1861 ggaaaaacca ccacactcaa aatgctcagc ggacttttgc accccacatc tggaagttta
     1921 tcggtgttgg ggtatgagcc tgctaaacgc aaccacgatt atttacgcca aatcacgtta
     1981 gtgatgggaa accgcaatca actctcttgg gatttgcctg cccttgattc atttgatttg
     2041 cagcgtgcca tttatggcat tccaactgat gagttcaaac gcactcgtga tgaattcatc
     2101 gagttgttag acttgaaaga attggtacag aagcctgttc gcaatctctc ttt
//
LOCUS       DAOQNJ010000496         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_42292_length_2150_cov_9.803819, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000496 DAOQNJ010000000
VERSION     DAOQNJ010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42292_length_2150_cov_9.803819"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..736)
                     /locus_tag="PLG52_12120"
     CDS             complement(284..736)
                     /locus_tag="PLG52_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB42240.1"
                     /translation="MNYFLRSATIQDAPAVTACVNHAFGHYVERIGMKPAPMEMDYEH
                     EIREHQVFVVEDAGQVVGSLVLGITQEGFLLDVIAVEPKYWGKGVGRIMLEHAEAEAK
                     QQGFDSIYLFTHEKMVENQALYKKVGYVEYDRRLEMGRNRVYMRKQLV"
     gene            complement(771..2033)
                     /locus_tag="PLG52_12125"
     CDS             complement(771..2033)
                     /locus_tag="PLG52_12125"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNB42241.1"
                     /translation="MDTISKQTYRRFLLGSQGLWPGRRFSGLSGTESALRQMQALQLD
                     PLVMVARSQDIAMYGRVLDYKPAMLYKMAYEKRKAFDYGGWLMMYPMQEFPYWQLHMQ
                     RRKEQGLRWEHFTHPPKQVVKFVLDELREKGPLGNRDIEGDAVKAWSYRGRKETSITL
                     FYLWLVGEVMISNRKGFDRLYDLRERVLPAEYDYAAPAEEAEDFFAKKHIAFSGIARE
                     PSWKTGVGDFIHRKVTAKEGATWIERLIEKGDIARVGVEGFRENFVVLSSDQAHLSVL
                     EAGRIPKAWKHKGPSTSEEVTFLAPLEIVSARGRAKKVFDFDYVWEVYKPLHQRKWGY
                     YTLPILYGDDLVARMDPKHDRTTNTLQILGFWLEEDAPRDADFANALAKGLKRFAEMI
                     GATKIDLVGVKPVKLKSELKMLLKRIGF"
BASE COUNT          584 a          541 c          466 g          559 t
ORIGIN      
        1 gccccagtgg attcacctga aaccatcttg gcatcctctc gaattctttt tgcctcttct
       61 gcatacagag gttgtaaaaa ccttgcttag gtcttgcgaa ttcgatgatt taaattttca
      121 cattgggaga tttaaacagg atggctcaga ataaaaataa tttcaatgaa tgaaaagtcc
      181 caatgccttg ttaatataaa ttgtcttgcc gttgaagtca agcaattaga ccaaattggc
      241 tgaataatcc gagtgagagg tctattttca aaaaccgttt agtctaaaca agttgtttgc
      301 gcatatatac tcggttacgt cccatttcaa ggcgacggtc atattcaaca tatccgacct
      361 ttttatacag agcttgattc tcaaccatct tctcatgagt aaagagatag atactatcaa
      421 aaccctgttg cttggcttct gcttcagcat gctccagcat gatgcgccca acacctttac
      481 cccaatattt tggctctact gcgatcacat ccagcaaaaa gccttcttgg gtaattccca
      541 aaacaagtga gccaacaacc tgcccagcat cttcaacaac aaagacctga tgctcacgaa
      601 tctcatgttc gtaatccatc tccataggag caggcttcat gccaatccgt tccacgtaat
      661 gaccaaaggc atgattcaca caagccgtaa cagcgggagc atcttgaatc gtagcggagc
      721 gtaagaagta attcatgtat cattttccta aagataggtt actaagaagg tcaaaaacct
      781 atccgcttga gaagcatctt taattctgat ttgagcttaa caggttttac accaaccaaa
      841 tcgatctttg tagcgccgat catctcagca aaacgcttca gtccctttgc aagggcgttc
      901 gcaaaatcag catctcttgg ggcatcttct tccaaccaaa agccgaggat ctgcaaggta
      961 tttgtggtgc ggtcatgctt tgggtccata cgtgcaacca agtcatctcc atataaaatc
     1021 ggcaatgtgt aatagcccca cttgcgttga tgcaaaggct tatacacttc ccacacataa
     1081 tcaaagtcaa aaactttctt ggcgcgcccg cgagcgctga caatttccaa cggagccagg
     1141 aatgtgacct cttccgatgt ggatggaccc ttatgcttcc acgccttggg gatgcgcccc
     1201 gcctcaagaa cagacaagtg ggcttggtcg ctgctcaaca caacgaagtt ttctctgaag
     1261 ccttccactc ctactcgagc tatgtcgcct ttttcaatga gacgttcgat ccatgtggca
     1321 ccctcttttg cggtgacttt acgatggatg aaatcaccga cgccggtctt ccatgaaggt
     1381 tcgcgcgcaa tgcctgagaa agcaatatgc ttttttgcaa agaaatcttc ggcttcttca
     1441 gcaggagcgg cataatcata ttccgctggc aagacgcgtt cgcgcagatc gtagagacgg
     1501 tcaaaacctt tgcggttgga aatcatcacc tcacccacca gccagagata aaacaaagtg
     1561 atggatgttt ccttacgccc acggtagctc catgctttga cggcatcgcc ttcaatatca
     1621 cgattcccaa gcggaccctt ttcgcgcagc tcgtccaaca cgaacttaac gacttgttta
     1681 ggtgggtgcg tgaaatgttc ccagcgcaaa ccctgttctt tacgtcgctg catgtgcagt
     1741 tgccagtatg gaaactcctg catggggtac atcatcaacc agccgccgta atcgaaggct
     1801 tttcgttttt catatgccat tttgtatagc atagcgggtt tgtaatcgag gacacgtcca
     1861 tacatggcta tatcctgact gcgagcaacc atcacaagtg ggtcaagctg caacgcctgc
     1921 atctggcgca aagcggattc ggtgccagaa aggcccgaga aacggcgccc aggccaaagt
     1981 ccctgtgagc cgagaagaaa gcggcggtat gtttgcttgg agatagtatc catggttaag
     2041 agtctacagt caaaggtcaa gataaaaaag accgaggcag gaacctcggt ctgtggtcaa
     2101 tcgtttatcg tctgttaatc gtcgccaagg gaagagattg cgcgaagcaa
//
LOCUS       DAOQNJ010000497         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_42589_length_2142_cov_7.332056, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000497 DAOQNJ010000000
VERSION     DAOQNJ010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42589_length_2142_cov_7.332056"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLG52_12130"
     CDS             complement(<1..404)
                     /locus_tag="PLG52_12130"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNB42242.1"
                     /translation="MSQAAKAQQLLSLHNNGKLLILPNVWNPIGARMLQAKGCSAVAT
                     ASAAIAESLGYSDGEQLKLDTMLDMVTRIVRSVDLPVTADFEAGYSDSIEGLQENISR
                     LLDTGAVGLNFEDSFDDSAHLRPIPEQVERIKA"
     gene            complement(422..1360)
                     /locus_tag="PLG52_12135"
     CDS             complement(422..1360)
                     /locus_tag="PLG52_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNB42243.1"
                     /translation="MHTHSHTHIGDLTQQTTKRLTLSLVLTAAFVIVEIVAGVFGNSL
                     ALLTDAAHNFTDVIALGLSWYALRIAMKPAHAGKTFGYHRVGILVALINSTTLILIAF
                     GIFFEAYHRFLTPPEVDSTILIGVGAVAFIINLVTALMVKEGSEHDLNMRSAFLHLMG
                     DVMSTLGAVIAGIIIAFTKWNWLDPLVSVLIGAFILYNAWGILKQTIHILLESTPENI
                     NVDNMVLDLQNINGVLGVHDLHVWSISENLRMLSAHVVIGDTTIGEGVSIQQNINELL
                     AHSYNIHHATLQMECEGCTSKDLFCQITEAHQHAHH"
     gene            complement(1364..1654)
                     /locus_tag="PLG52_12140"
     CDS             complement(1364..1654)
                     /locus_tag="PLG52_12140"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB42244.1"
                     /translation="MKLPELKAIKLAELFSSLSDASRIRIIASLLDGEMSVRVLAERL
                     GMTESAVSHQLRGLRQMHLVRARKSGRQVFYSIEDDHVARLFTLGLDHVQHG"
     gene            complement(1736..>2142)
                     /locus_tag="PLG52_12145"
     CDS             complement(1736..>2142)
                     /locus_tag="PLG52_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42245.1"
                     /translation="NIFKSTSTELSFCGIRGANQELPAGCGFSGKWIVPSKHVFLPGS
                     YMILKQTGENVSGEIFDGNGNPYESFNGTMEWGKGWRANGTYKERGDISLWINASETG
                     FEFVNPKGNIQQLCAVREGVASAYLGSYSCEP"
BASE COUNT          565 a          472 c          553 g          552 t
ORIGIN      
        1 gctttgatgc gctccacttg ttcagggatg gggcgtaggt gggcgctgtc gtcgaagcta
       61 tcttcaaaat tcaagccgac agccccggta tcgagcaacc gtgaaatatt ttcttgtaaa
      121 ccttctatac tatcggaata ccctgcctca aaatctgcgg tgacgggcag atctacactg
      181 cggacgatac gagtgaccat atctagcatg gtatccagtt tgagttgttc gccgtcgctg
      241 tagccaagag actcggcaat ggctgcgctg gctgtggcta cagccgagca ccctttggct
      301 tgcaacatcc gtgcgccaat gggattccac acattaggca gaatgagcag tttaccgttg
      361 ttgtgtaagg ataaaagttg ttgtgctttt gcggcttgag acatgggact ccaaataaaa
      421 gttagtggtg ggcgtgttga tgtgcctcag tgatctggca aaacaagtct ttggatgtac
      481 agccttcaca ctccatttgt aaagtggcgt gatggatatt gtaactatgg gcaagcagtt
      541 cattaatgtt ttgttgaata gagacgcctt cgccaatggt agtatcgccg ataaccacat
      601 gcgctgagag catgcgcaag ttttcactga tgctccacac atgcaggtca tggacgccta
      661 aaacaccgtt gatgttttgc aggtcaagca ccatgttatc tacatttatg ttttcgggag
      721 tgctttctag caagatgtga atggtctgct tcaggatacc ccaagcgtta tataagataa
      781 aggcaccgat aagaacacta acaagcgggt cgagccagtt ccattttgtg aaggcgatga
      841 tgatacctgc aatgaccgcc ccaagtgtgg acatgacatc acccatgaga tgaagaaaag
      901 cgctgcgcat gttgagatca tgttcgctgc cttctttgac cattaatgcg gtgacgaggt
      961 tgatgataaa agccacagcg ccaactccga tcaggatagt cgaatcaact tcgggcggag
     1021 tcaagaagcg gtgataggct tcgaagaaaa tcccaaaggc gataagaatt agcgtcgtcg
     1081 agttaataag cgcgacaaga atcccaacac ggtgatagcc aaaggtcttg ccagcgtgag
     1141 cgggtttcat ggcgatgcgt aaggcatacc aacttaagcc taatgcaatg acatcggtga
     1201 agttatgggc tgcatcggta agcaaagcaa gactgttgcc aaaaacacct gcgacgatct
     1261 caacaatgac aaatgctgca gtgagaacta aggagagggt gaggcgtttg gtggtttgct
     1321 gggtaagatc gccaatatgg gtatgagaat gagtgtgcat gggttatccg tgctgaacgt
     1381 gatcgagacc cagcgtaaat agccgcgcta cgtggtcatc ttcgatggaa taaaaaactt
     1441 gtctgccaga tttgcgtgcg cgaacgagat gcatttggcg caacccacgg agctgatgcg
     1501 atacagcgga ttcggtcatg ccaagtcttt cagcaagcac gcggacgctc atttctccat
     1561 ccaagaggga tgcgatgatg cgaatgcgac ttgcatcgct tagggaactg aatagttcgg
     1621 caagtttgat ggctttgagt tcgggtaatt tcataattga atatatgaac aactattcat
     1681 atattactct gtaaaaaaga ccctgtcaat atggtgtttg gcagggtctg agtaattacg
     1741 gttcgcagga ataactgcct agatatgcac ttgctacgcc ttcacgcact gcacaaagtt
     1801 gttgaatgtt acctttcggg tttacaaatt cgaagccggt ttctgaggca tttatccata
     1861 gcgaaatatc gccgcgttcc ttgtaggtgc cattggcgcg ccagccttta ccccattcca
     1921 ttgtgccatt gaaactttcg taggggttgc cattgccgtc aaagatttca ccactcacat
     1981 tttcaccggt ctgcttcaag atcatatagc ttccgggcag aaaaacgtgt tttgaaggga
     2041 caatccactt tccactaaag ccacagccgg caggaagttc ttggttagca ccgcgaattc
     2101 cgcaaaatga taattcggtc gaggtggatt tgaagatgtt ct
//
LOCUS       DAOQNJ010000498         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_42647_length_2140_cov_8.975540, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000498 DAOQNJ010000000
VERSION     DAOQNJ010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42647_length_2140_cov_8.975540"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PLG52_12150"
     CDS             complement(<1..879)
                     /locus_tag="PLG52_12150"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB42246.1"
                     /translation="MRTIVRSISTLLLLAVLFSLAPGGTTTARAASCNWAQFVADVTI
                     PDGTSFAPNTAFKKTWRLKNIGTCAWNSNDVSLIFDSGEKMGAPASLALPTTVNPGQT
                     VDITVDMTSPAAAGHYFGYWKFKSNSGGTFGIGSTAQKSFWVEINVTSSAGTGYDFTA
                     NAASATWSSGAGALTFPGTDGNANGFGLSQATPKLESGVTSAQPGLLFAPNNVTNGYI
                     QATYPAFRVQAGDRFQATIGCEAAATNCYVAYSLNYQIGSGAVKTLWTFREKYEGMTY
                     NVNLDLSSLANQDVKFT"
     gene            1068..>2140
                     /gene="dinB"
                     /locus_tag="PLG52_12155"
     CDS             1068..>2140
                     /gene="dinB"
                     /locus_tag="PLG52_12155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNB42247.1"
                     /translation="MARTILHLDLDAFYCSVEETQNPQLRGKPFAVGGRPDERGVVAS
                     CSYAARALGIRSAMPMSRAVRLCRDLIIVPGRHHLYGEYSAKVMEKLHNLTPLVEQIS
                     IDEAFLDISDIQADKTRLALDLQLSIRNELNLPSSIGIASNKLVAKIATEVGKKSSKK
                     KNEPPFGFTVVPVGDEAKFLDPLPADMLWGVGPKTNARLAELGIHTIGDIARWPEKEL
                     VSLFGENGRDLWQHAHGMDNRPVSTESETKSISQETTFNVDISDQKTLEKTLREQAHE
                     VARQLRKNDLAGKTVKIKIRWPDFTTLTRQTTLPTSTDNEDDIYRAAVKLMDSVRKPN
                     QAVRLLGVGVSGIGAPVRQLSLW"
BASE COUNT          586 a          561 c          497 g          496 t
ORIGIN      
        1 agtgaatttc acatcttgat tggcaagcga gcttaagtcg aggttgacgt tataggtcat
       61 accctcatat ttttcacgga aggtccataa ggtcttcacc gcgccagacc cgatctgata
      121 gttcaagcta taagctacat aacaattggt tgcagcagct tcacaaccga tggtcgcttg
      181 aaagcggtct cccgcttgaa cacggaatgc tggatacgtt gcttgaatgt agccattggt
      241 tacattgttc ggcgcaaaaa gcaaaccggg ttgagcagag gtcacgccgc tttcaagctt
      301 cggcgtagcc tgactcaaac caaaaccatt ggcattgcca tcggtaccgg gaaaggtcaa
      361 cgcacccgca ccgcttgacc atgtagcaga agccgcattt gcagtaaagt catacccagt
      421 tccggcagag gaagttacgt tgatttcaac ccagaaagat ttttgggcag tagacccaat
      481 accgaaagtt ccgccactat tgctcttaaa cttccagtag ccgaagtaat gccccgccgc
      541 tgcaggcgaa gtcatatcta cagtgatatc cacggtttgg cccgggttaa cagtggtcgg
      601 caaagcaagc gaagcgggcg cgcccatctt ttcgccgcta tcaaagatca gcgaaacatc
      661 gttgctattc caggcgcatg taccaatatt cttcaaacgc caggttttct tgaaagccgt
      721 atttggcgca aaactggttc catctggaat ggtcacatct gcaacgaact gcgcccaatt
      781 acaagacgca gcccttgcag tcgttgtacc acctggggcg aggctgaata gcaccgccaa
      841 caggagtaat gttgaaatcg atctcacaat agttctcatt tcttcctcca tcagtcatac
      901 ggggtatgga cgtagtgatt aagtgaaaag ttcccttttg actatacgct accatagtat
      961 aacttttctt taataattgt tcaataatga aacagaacaa aatcatagtc aatttttccc
     1021 atttttctgt gctcttaggg caaaatcgac tgcactataa taccaacatg gctcgcacga
     1081 tccttcacct tgacctcgat gcattttatt gctccgtgga agagacccaa aacccgcaac
     1141 tgcgcggcaa accgtttgcc gttggtggca gacctgatga acgtggtgtg gtcgcatcat
     1201 gttcatatgc ggctcgcgcg cttgggattc ggagtgccat gcccatgtcg cgagcggtgc
     1261 gcctatgtcg tgatttgatc atcgtacccg ggcggcacca tttgtatgga gaatactctg
     1321 ccaaggtgat ggaaaagctg cataacctga ctccactcgt ggagcaaatt tccatcgacg
     1381 aagccttcct cgacatctcg gatattcaag cggataaaac ccgcctcgcg ctcgacctgc
     1441 aactgtccat acgaaatgag ctgaatctac cctcgagcat tgggatcgcg tccaacaaac
     1501 tcgtggcaaa aatagccact gaggttggta agaaatcaag caagaaaaag aatgagccac
     1561 cattcggctt caccgttgtt cctgttggag atgaagccaa atttctagac ccactccccg
     1621 ctgatatgct ttggggtgtg ggaccaaaaa ccaatgcaag gttagccgag cttggcattc
     1681 ataccattgg ggatattgcc cgctggcccg aaaaggaact cgttagcctc ttcggcgaaa
     1741 atggacgtga cctctggcaa catgcacacg gcatggataa tcgtccagtc agtaccgaat
     1801 cagagaccaa gtctattagc caggaaacca cgttcaacgt agatatcagc gaccaaaaga
     1861 ccttagaaaa aacattgcgc gaacaagctc acgaagtggc tcggcaactt cgcaaaaatg
     1921 acctagccgg aaaaacagtc aaaataaaaa tccgctggcc cgacttcacc accctaacaa
     1981 gacaaaccac cttacccact tctacagaca acgaagacga tatctaccgc gccgccgtca
     2041 agttgatgga ctctgtccgc aagccaaatc aagccgtgcg gttgttagga gtcggcgtca
     2101 gcggaatcgg agctccggtt cgtcaactca gcttatggga
//
LOCUS       DAOQNJ010000499         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_42908_length_2133_cov_7.657363, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000499 DAOQNJ010000000
VERSION     DAOQNJ010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42908_length_2133_cov_7.657363"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLG52_12160"
     CDS             <1..447
                     /locus_tag="PLG52_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42248.1"
                     /translation="SYAEFRPPTGNNGTYGYVASIPVPSVLYGTGMYLDGFRMCYDAT
                     DPGTTLYDAHIYLIDGSSGGSSAQFTDFTDRNDELCRTYGLPTPTLFHADDYVLIFLN
                     VNFAGTNDFFRIRSLNINLHPSPTGETVLKQPGEDVTPLMPFTEEP"
     gene            836..1567
                     /locus_tag="PLG52_12165"
     CDS             836..1567
                     /locus_tag="PLG52_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNB42249.1"
                     /translation="MNRKFGILFSFLGVLLLAAGLFTSRAAAATPDCFSDVTTHWAED
                     FICWMKDNGLTNGYPDGTFKPDNSITRAEVAVFMQKIRTTGVVQFNTGPTGFQPLVGD
                     NNGYVQYDFGATRLKSSVGGFNNRFVSSISIPSSLYNSRMYVNSVKICYTLQGSGYIS
                     QANFSVYSTDGSSISSLIDPTDLTTDGCTTYNFPTPVGLDGGEYLQTIVNSAGTSAIT
                     DWVNIHSITVYMTPSTEAAVAKEAE"
     gene            complement(1657..>2133)
                     /locus_tag="PLG52_12170"
     CDS             complement(1657..>2133)
                     /locus_tag="PLG52_12170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB42250.1"
                     /translation="DALTGALNRHTLVPTLEHEISRALRYERPLTLILLDIDHFKDIN
                     DTFGHLVGDHVLIVVASLLRDSIRSTDYLGRWGGEEFLIILPETEALVAEKLAERLCD
                     SIRQKQYGEVEQVTASFGVTTYKTGQTLEDVQQAVDMAMYQAKKGGRDQVVSFPST"
BASE COUNT          565 a          578 c          430 g          560 t
ORIGIN      
        1 agctatgcag aattccgccc gcctacaggc aataatggta catatggcta tgttgcttcc
       61 attcccgtac caagcgttct ttatgggaca ggcatgtatc tggatggatt ccgcatgtgt
      121 tatgacgcta cggatccagg gacaactctt tatgatgctc atatttatct gatcgatggt
      181 tctagtggtg gctcttctgc acaattcaca gattttactg atcgtaacga cgaattgtgt
      241 cggacttatg gactacccac accaaccctt ttccatgccg atgattatgt attgattttc
      301 ctcaatgtga attttgcagg tacaaacgac ttcttccgca tccgctccct caacatcaat
      361 ctgcatccca gccccactgg cgagacagtc ctcaagcaac ccggtgaaga tgtcacacca
      421 ctgatgcctt tcactgaaga accgtaatat tcaaagcaca aaacaaactc acctcgaagc
      481 cttaatttcg aggtgagttt gtttttaggc tggggattat ccataggttg ctgcttttat
      541 tcactcgtat tttaaatact taaacccatg acctatgtgg tcggtcgaaa taaattttac
      601 aaactttctc tgctctggca ggggcttagc ccctgccaaa gccacggaga tttctaaaga
      661 tatacaaccg actactcatg tccataaggc ttaattcaaa ggcgcacctc acaccaatgt
      721 actatgaaaa aaagctcaaa acccaccaat ggtcatattg aaatcaaaac caaaatgcgt
      781 acaatgacca aacatcgtca aatgtccgcg atgattttat taataggaga aaaaaatgaa
      841 tcgaaaattt ggtatcttgt tctcgttttt gggtgtgctg ttgctagccg ccggtctctt
      901 taccagccgc gcagccgccg ccaccccaga ctgcttctcg gatgtcacca cacattgggc
      961 agaagatttt atttgctgga tgaaagataa tggcctcacc aacggttatc ccgatggtac
     1021 ctttaaaccc gataattcca tcacccgtgc tgaagtggct gtattcatgc aaaagatccg
     1081 taccacgggt gttgtgcaat tcaacaccgg ccccaccggc ttccagcctt tggttggtga
     1141 caacaatggt tacgtccagt atgattttgg cgccacacgg ctcaagtcct ctgttggcgg
     1201 tttcaacaat cgttttgttt cctccatctc cattccaagc agcttgtata actccagaat
     1261 gtacgttaac tctgtaaaga tatgctacac ccttcaaggc agtggatata tttcgcaggc
     1321 aaatttctca gtctattcca cagacggaag ttccatatcc tctctaattg accccacgga
     1381 tctcaccacc gatggctgca ccacctacaa ttttcccacc ccggtcggtc tggatggcgg
     1441 tgaatatctg caaaccatcg tcaactccgc cggaacctcc gccattacag actgggtcaa
     1501 catccactcc atcacggtat acatgacgcc cagcacggaa gccgcagtgg ccaaagaagc
     1561 tgaataaatt tcctaccata aagacccctc tccatttcag agaggggtct cgcatttaac
     1621 tatatctatc tcgtacttcc agacataatt tcccgatcag gttgagggaa aacttacaac
     1681 ttgatcacgc ccgccttttt ttgcctgata catggccata tcaacagcct gctgcacatc
     1741 ctccaacgtc tgccccgtct tataagtcgt taccccaaaa ctggcggtta cctgctccac
     1801 ttccccatac tgtttttggc gaatcgagtc gcagagccgt tcggcaagtt tttccgcgac
     1861 cagggcctct gtctcaggca aaatgatcag aaattcctcg ccgccccaac ggcccaaata
     1921 atctgtcgaa cggatcgaat cccggagaag ggaagccaca acgatcaaca catggtcacc
     1981 cacaaggtgt ccgaaagtat cattgatatc cttgaagtga tcgatatcca aaaggatcaa
     2041 ggtcagcggc cgttcgtagc gtaacgcacg agatatttcg tgttcgagag tgggtacaag
     2101 agtatggcga ttaagcgccc ccgtcaaagc atc
//
LOCUS       DAOQNJ010000500         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_43339_length_2120_cov_6.647942, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000500 DAOQNJ010000000
VERSION     DAOQNJ010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43339_length_2120_cov_6.647942"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..594)
                     /locus_tag="PLG52_12175"
     CDS             complement(118..594)
                     /locus_tag="PLG52_12175"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNB42251.1"
                     /translation="MKKPNWMIVAILFGVSLFLGYAIFTALATAVFPAVNNISGKFLC
                     NGEYEVVTTKSTPRPGETYWSHSILCDGKDITLPAVAITGLMASSIFFVLLLIFTRNW
                     WFREKAAPAPFAKAGVTEKGKKSALDRMAELKEMRDKNLISQVEYERKKDEIMKEL"
     gene            complement(604..1347)
                     /gene="budA"
                     /locus_tag="PLG52_12180"
     CDS             complement(604..1347)
                     /gene="budA"
                     /locus_tag="PLG52_12180"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNB42252.1"
                     /translation="MERTTIRRSRKRRADSKIYLCAPVNSLVEGIYEQNIPFHEIRKH
                     GDMGLGTFNDLDGEMMMVDGEIYRVGSDGKVNLITDDDMCTPFACVTFFQPASEETLE
                     TEMDYEQFLRWMMELMPSPNIFYAFRITGEFAYVKTRSVPKQENYRPLVEVAKEQPTF
                     EFHNVSGKMVGFYTPSFVSALSVPGVHLHFLTEDKTSGGHLLACKPRKVKVELQYLYT
                     VELSLPANLDYLTWDFQRDIGKDLDKAEK"
     gene            complement(1450..>2120)
                     /locus_tag="PLG52_12185"
     CDS             complement(1450..>2120)
                     /locus_tag="PLG52_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNB42253.1"
                     /translation="GVFMIVVSDMMGTLTTGSPFRGLVDWVKHNQSKSRANWYMASIA
                     PSYFLAKRGIIDWQDWGHRLMINSLSYIKDATPEKVSHAAEWVVEKDLWVKRREDVVA
                     RLIKHREEGASIYIASSYFEPFLEPFARRIGAKGIGTPVELVDGQLRMTSKQMVADER
                     KLEQVLSRLGVERVDVAYGDTILDVPLLEHADHPVAVYPDAKLKKIALERGWEIIGDT
                     PKYS"
BASE COUNT          541 a          557 c          465 g          557 t
ORIGIN      
        1 gcgaaaggac aacggctttc aacgatttgg tgaagagaaa gcctgtgagt tcgatcccct
       61 ctcccggtgt gagatgaggc gcgagttcct tgtggatgaa ttcgtcaaat ttatgcatca
      121 cagctccttc atgatctcat cttttttacg ttcatattcc acttgagaga tcaaattctt
      181 atcacgcatt tccttaagtt ctgccatacg gtcaagcgca gatttctttc ctttttctgt
      241 caccccggct ttggcaaatg gagccggagc agctttttca cggaaccacc aattacgcgt
      301 gaagatgagc aacaacacaa aaaatataga ggatgccatc aaacccgtta ttgcaacagc
      361 gggaagtgtg atgtccttac catcacagag gatgctatga gaccaatacg tttcaccggg
      421 gcgcggagtg gattttgtgg tcacaacttc atattcgccg ttgcaaagga acttgcccga
      481 aatgttattg acagcaggaa agaccgcagt ggctaacgcc gtaaagatcg cataccctaa
      541 aaacaaacta acaccaaaca atattgcgac gatcatccaa ttaggttttt tcataacgac
      601 ttcctatttt tctgccttat ccaaatcttt gccaatatcg cgttggaaat cccaagtaag
      661 gtaatcaaga tttgcaggca gcgaaagttc taccgtataa agatattgca actccacttt
      721 taccttgcgc ggcttgcatg ccaacaaatg tccaccgctg gttttatctt cggtaagaaa
      781 atgcaaatgc acaccaggca cactgagtgc agagacgaat gatggcgtat agaacccaac
      841 catctttccg cttacgttat gaaattcaaa ggtcggctgt tccttcgcca cctccaccag
      901 cggacgatag ttctcctgct tcggcaccga gcgcgtctta acatatgcaa actcacccgt
      961 aatgcgaaaa gcataaaaaa tattcggcga gggcatcagt tccatcatcc aacgcaggaa
     1021 ctgctcgtaa tccatttcag tttcaagcgt ttcttcactg gcaggttgaa agaaggtaac
     1081 acaggcgaat ggtgtacaca tatcatcatc ggtgatcagg ttgaccttac catcagaccc
     1141 cacacgataa atttcaccat ctaccatcat catttcgccg tcgaggtcgt tgaaggtacc
     1201 caatcccata tcgccatgtt tgcgaatctc atggaatggg atgttctgct cataaatgcc
     1261 ttcgaccaat gaattgaccg gcgcacataa atagatcttg ctatctgccc gcctctttct
     1321 ggacctgcga atggttgttc gttccatttt tcctctttct tgtcattgcg aggaggcgtt
     1381 ttttgccgac gaagcaatct catcctttac gtggagattg cttcgccgct atcgcggctc
     1441 gcaatgacgt taactgtatt tcggcgtgtc gccgataatt tcccagccgc gttcgagcgc
     1501 gatcttcttc aatttggcat cggggtaaac tgccacggga tgatcggcat gttccaacaa
     1561 cgggacatcc aggatcgtat cgccgtacgc tacatccacc cgctccacgc cgaggcgact
     1621 gagcacctgc tccagcttgc gttcgtcggc taccatttgc ttgctggtca tccgtaattg
     1681 tccatctaca agttctacag gagtaccgat ccctttcgcg ccgatccgtc tcgcaaaggg
     1741 ctcaagaaag ggttcaaagt aactgctggc aatgtaaatg gatgcgcctt cttcgcgatg
     1801 ctttatcaaa cgggcgacca catcttctcg gcgctttacc cataaatctt tttccaccac
     1861 ccattcggcg gcatgggaga ctttttccgg cgtggcatct ttgatataag aaaggctgtt
     1921 gatcatcaaa cgatgccccc aatcctgcca gtcaatgatg ccgcgtttgg caaggaaata
     1981 cgaaggcgca atggatgcca tgtaccaatt ggcgcgagac ttgctttggt tatgcttgac
     2041 ccaatccacc agcccgcgaa agggcgagcc ggtggtgagg gttcccatca tatctgaaac
     2101 gacgatcata aatactccta
//
LOCUS       DAOQNJ010000501         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_43377_length_2119_cov_10.849806, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000501 DAOQNJ010000000
VERSION     DAOQNJ010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43377_length_2119_cov_10.849806"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..646)
                     /locus_tag="PLG52_12190"
     CDS             complement(38..646)
                     /locus_tag="PLG52_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42254.1"
                     /translation="MIPARLSPRLMNRFVETLSKEVGADTYHAVLSNSGLSRDWQKPE
                     FFLAFDNKQTAEAYAKLQSALRTYYGRGARGILLRIGSKLWEPMLNDASFASKAQATL
                     IRGFPKPLKRKPALELLASTLGAGRGDVTVHTLDLDLLLVDHSSPTTLGQKENSPVCF
                     VTLGLIRECLYWATGDEHDIEELVCKAAGSHQCEFKITIGAS"
     gene            complement(646..1509)
                     /locus_tag="PLG52_12195"
     CDS             complement(646..1509)
                     /locus_tag="PLG52_12195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB42255.1"
                     /translation="MINLHLHIRRAVAQDHRQLSNLLFQESNTHRHLDWRTALEWIGV
                     HNFWVLEDSGTVVAALACPEDPPDIAWIRLFSFSPHLSGPEAWSDLWNFASAEIRAIN
                     PRAEAASIVMKQWFQTLLLDSGFEPRLSIVLLELKSEDYVPVRISDAIRMRPMQEADL
                     GIVEDVDREAFGKFWHNTSDALQRAHGQSFYSSVAEDANGIIGYQISTGNPFGVHLAR
                     LGVRPEAQGKGVGSALVHDLIQRAGGLQSGRLSVNTQDDNVASLRLYSKLGFLRTGEH
                     YPVFIHSLGAS"
     gene            complement(1506..2027)
                     /locus_tag="PLG52_12200"
     CDS             complement(1506..2027)
                     /locus_tag="PLG52_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42256.1"
                     /translation="MSLEPHQLDAKGKQAFAKKNFVEAAEYFRQAAEGYTQGRAGLEA
                     AESRNNLSVALLQAGKPQEALDAALDTDKVFEGAKDVKRQAMAFGNQAAALEGLHRYD
                     EAIQKYELSAELFGSIDEGDLRAMVMKSAAAIKLKTGKITDSAFKMMGSLDVKKNPTF
                     FERIMKFLMKFIR"
BASE COUNT          580 a          493 c          497 g          549 t
ORIGIN      
        1 tcttttgctt tccaattaaa ttcttgatgt ttcatgtcta tgatgctcca atcgtgatct
       61 taaattcaca ttgatgagac cccgccgctt tacagacaag ttcttcaatg tcatgttcat
      121 cgccagttgc ccaatataaa cattcacgga tcaagcccag ggttacaaaa cacacagggg
      181 aattttcttt ttgtcccaac gtggtaggag aagaatggtc aaccagaagc aggtcaagat
      241 ccaaagtatg aaccgtgaca tcgccgcgtc ctgcaccaag ggttgaagca aggagttcaa
      301 gcgcaggttt acgcttcaat ggtttgggaa acccgcgtat gagagttgcc tgagctttag
      361 aggcaaaaga agcatcgttc aacatgggtt cccaaagctt agaaccgatt ctcaaaagga
      421 taccgcgtgc gccgcgccca taataggtgc gcaaagctga ttgcaatttg gcatatgctt
      481 ctgcagtttg tttattatcg aaggcaagaa aaaattctgg cttctgccaa tctcgtgaca
      541 aaccagaatt tgataaaact gcatgatagg tatcggcgcc cacttctttt gaaagagttt
      601 ctacgaagcg attcattagg cgtggagaaa gtcgggcagg tatcactatg acgctcccaa
      661 ggaatggata aaaacagggt agtgctctcc tgtgcgaaga aagccgagtt tcgaatacaa
      721 acgcaatgat gcgacgttat catcctgcgt gttgacagat agtctaccag actgaagccc
      781 gcctgctctt tggatcaaat catgtacgag ggctgagcca actcccttgc cttgcgcctc
      841 agggcggacc ccaagccttg caagatgcac cccaaacgga ttccccgtac tgatctgata
      901 tccaattatt ccattggcat cttcagcgac agaggagtag aaagattgac catgtgcacg
      961 ttggagagca tctgaagtat tgtgccagaa ttttccaaag gcttcacggt ctacgtcttc
     1021 aacaatacca aggtctgcct cttgcatcgg tcgcattcga atagcatcac tgattcgaac
     1081 aggaacataa tcttcacttt tgagttcaag aagaacaata cttaagcgtg gttcaaaccc
     1141 actatctaaa agcagcgttt gaaaccactg tttcattaca atcgaagctg cttcagcacg
     1201 cgggttgata gcccgaatct cagcggaggc aaagttccat aggtcagacc aagcttcggg
     1261 tccagaaaga tgcgggctaa atgaaaatag acgaatccat gcgatatctg gcgggtcttc
     1321 agggcaagcc aaggcagcca ccacagttcc cgaatcttcc aatacccaaa aattatggac
     1381 gccaatccac tcaagggctg tgcgccaatc gagatggcgg tgtgtatttg attcttgaaa
     1441 taggagattg gaaagctgac gatggtcctg cgcaacggcg cgccggatat gcaggtgaag
     1501 attgatcatc tgataaattt catgagaaac ttcatgatac gttcaaaaaa ggttggattc
     1561 ttttttacgt cgagcgagcc catcattttg aaagcagaat cggtgatctt gcctgttttg
     1621 agtttgatgg cagccgcaga tttcatgacc attgcgcgca ggtcaccttc atcaatactg
     1681 ccaaacaatt ctgcagagag ttcgtatttt tgaatcgctt catcatatcg atgaagacct
     1741 tcgagcgccg cagcttggtt accaaacgcc atggcttgac gtttcacatc tttggcacct
     1801 tcaaagactt tatcggtatc aagagctgca tcgagtgctt cctgaggttt gcctgcttgc
     1861 aagagtgcaa cacttaagtt gttacgagac tctgctgctt caagcccggc acgtccttgg
     1921 gtgtaccctt cagctgcctg gcgaaaatat tcagcagctt caacaaagtt ctttttggca
     1981 aaggcttgtt tccctttggc gtcgagttga tgaggttcaa gagacataat tattaaaggg
     2041 tgatgtcgag caagccttct gcaacagagc gaagtttatc gcgagacatg ctgctgagct
     2101 tcatggcgag ttcaagata
//
LOCUS       DAOQNJ010000502         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_43734_length_2109_cov_8.168939, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000502 DAOQNJ010000000
VERSION     DAOQNJ010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43734_length_2109_cov_8.168939"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PLG52_12205"
     CDS             complement(<1..541)
                     /locus_tag="PLG52_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNB42257.1"
                     /translation="MLTKILIANRGEIAIRIMRACRELGIKTVAVYSEADKNAQHVQF
                     ADEAILLGGAAPKESYLNVDALLRATRDSQADAIHPGYGFLSENASFAAAVESANLTF
                     IGPSADSIRAMGDKAESKILMKKAGVPTVPGYEGLESYDDFKNAASEIGYPVLVKAAA
                     GGGGKGMRVVNAESELQEAI"
     gene            complement(531..1517)
                     /locus_tag="PLG52_12210"
     CDS             complement(531..1517)
                     /locus_tag="PLG52_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB42258.1"
                     /translation="MKILIIGGTRFIGRHLVTSARARGHEVTLFNRGKSNPGLFRRIQ
                     NIRGDREKDLDQLSGQWDAVIDTCGYLPRVVRLSAEALKEKVSRYVFISSVSVYANFS
                     KVGIKESDAVGTLADESVEEITGETYGPLKALCEKAVQDVYGARSLIIRPGLIVGPHD
                     PTDRFTYWPLRIAKGGDVLTPDRPDAMAQFIDARDLADFIIRLIDQNVSGTFNAVGYP
                     VTLNTIFETCKRISKSNANFKWAPIDFLEKNNVAPWSDMPIWVPEVGEDAGVSQVDVS
                     KAVAAGLTFSPLTDTVKAIYDWEFERPAEYEMKAGLKPEREAELLKLLNHAH"
     gene            complement(1514..1993)
                     /locus_tag="PLG52_12215"
     CDS             complement(1514..1993)
                     /locus_tag="PLG52_12215"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HNB42259.1"
                     /translation="MTHKESSPHHFLAKLAGHWQGTSKLWFEPDKLANEAPIVGTIQI
                     ILDGRYALFLYQSSIEGEAQHGMFTFGYNTQLDRYETSWVDSFHTNTSIMFCTGNEIE
                     NGFSVLGSYPDPTGGPDWGWRTDVQMNGDELIVTAYNILPEGLEAKATEIILKKVNA"
BASE COUNT          499 a          530 c          545 g          535 t
ORIGIN      
        1 caatggcttc ttgcaactcg ctttcagcat tgaccacgcg catgcctttg ccgcctccgc
       61 ccgctgcggc tttgacgaga accgggtagc caatttcgct ggcggcgttc ttgaaatcgt
      121 cgtagctttc caagccttcg tagccaggga cagtgggcac gccagctttc ttcatcaata
      181 tttttgattc agctttatcg cccatggcac ggatcgaatc cgctgaggga ccgataaagg
      241 ttaggttcgc tgattccact gcggcggcga aagaggcgtt ctctgagagg aagccatagc
      301 ctgggtgaat ggcatcggct tgagagtcac gagtggctcg cagtaaggca tccacattca
      361 aataagattc tttcggtgct gcccccccaa gcaagatggc ttcatcggcg aattgcacgt
      421 gttgtgcgtt cttatccgct tcggaataga cggcgactgt tttgatgcca agttcacgac
      481 aggcacgcat gatacgaatg gcgatctcgc cgcgattggc gataaggatt ttagtgagca
      541 tggttgagta atttaagtaa ctcggcttca cgctcaggct tcaagcctgc tttcatttca
      601 tactctgcgg ggcgttcaaa ctcccagtcg taaatggctt tgacggtatc tgtgagcggc
      661 gagaaggtaa gcccggctgc taccgctttc gaaacatcca cctgcgaaac cccggcatct
      721 tcgccgacct ctggaaccca gatgggcata tcgctccatg gggcaacgtt gttcttttca
      781 agaaaatcta taggcgccca tttgaaattg gcgttgctct tactaatccg tttacaggtt
      841 tcaaaaatag tattgagcgt gacgggatac ccaacagcat tgaaggtgcc cgaaacattt
      901 tggtcaatga gccggatgat gaaatcggct aggtcgcgcg cgtcaataaa ttgagccatt
      961 gcatcggggc ggtctggagt gagcacatct ccacctttgg cgatgcgtaa aggccaatag
     1021 gtgaaacggt cagtggggtc atgcggaccc acaatgagcc cagggcgaat aatcagtgag
     1081 cgggcaccat atacatcttg cactgctttt tcgcacaaag cttttagagg accataggtt
     1141 tcgccagtga tctcttccac gctttcatcg gcaagggtgc caacggcatc gctttctttg
     1201 atgccaacct tgctaaagtt cgcatacacc gaaacgctgg aaataaatac ataacggctg
     1261 accttttctt tcagcgcttc agcagagagg cgcacgacgc gcggcaggta gccgcaggta
     1321 tcaatgaccg catcccactg ccccgataac tgatccaaat ctttttctcg atctccccga
     1381 atgttttgga ttcttcgaaa aagacccggg ttgctttttc cgcgattgaa gagcgtgact
     1441 tcatgtccgc gggcacgggc agacgtgacg agatgccttc cgataaaacg agtcccgccg
     1501 ataatgagga tcttcatgca ttcacctttt taagaatgat ttcagtggct ttggcttcaa
     1561 gcccttctgg caaaatatta taggcagtga cgattaattc gtcaccattc atctgtacat
     1621 cggtgcgcca cccccaatcg ggtccgccag tggggtcggg ataactgcca agcacagaga
     1681 atccattctc gatttcgtta ccggtgcaga acatgatgga tgtattggtg tggaaggagt
     1741 cgacccaact ggtctcgtag cggtcgagtt gggtgttgta gccgaaggtg aacatgccat
     1801 gctgggcttc accttcaatg gaagattgat ataggaagag cgcataacgt ccatcgagga
     1861 tgatttgaat ggttccaacg atgggagctt cgttagccag tttatcaggt tcaaaccaga
     1921 gtttactggt accctgccag tgaccggcaa gttttgcaag aaaatgatgt ggggaggatt
     1981 ctttatgtgt catggttaca tcctaaatac gccgaagccg ccatcttctt ttggcgcatt
     2041 cagcacaacg gataatgcta agcctaaaac ctgtcttgta tcggcagggt cgattacacc
     2101 atcatccca
//
LOCUS       DAOQNJ010000503         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_43893_length_2105_cov_7.611220, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000503 DAOQNJ010000000
VERSION     DAOQNJ010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43893_length_2105_cov_7.611220"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLG52_12220"
     CDS             complement(<1..605)
                     /locus_tag="PLG52_12220"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNB42260.1"
                     /translation="MDLEHQIGQKLLLAFRGKESLSPEIIQAFKNYHPSGVTLFRPFN
                     IESPAQIKKLNRSLQDLARELGLPLLLIATDQEGGQLMAFGDGTPLPGNMALGATRSP
                     ELARKAGEVLGRELAAIGVNVNYAPSADVNINPNNPVVGVRSFGEDPALVGELAAAMI
                     EGIQSQGVAATVKHFPGHGDTASDSHHGLGTVPHSRERLHAV"
     gene            complement(607..1449)
                     /locus_tag="PLG52_12225"
     CDS             complement(607..1449)
                     /locus_tag="PLG52_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNB42261.1"
                     /translation="MKKYVPILKSIVLYTLLTGATVLMSFPLFWMISSSFKTVEETNS
                     PGVVWIPREPTVEAYTAIFKNPDFLRSYFNSVFYVTLALVGTLLSIAAVAYAFSRVDW
                     PGRNVVFFLMLSTMMIPPQALIVPQYVFFNKLDWIGTFNPLIIPGYFAGGAAMIFLLR
                     QSMSQIPKELDESAFVDGANHIQIWWEIILPLVKAPMATVATFLFVGLWNSLLTPLMY
                     LQTVDLYTLPVYVSTLYNINQTVQPWPTIMTAAVLTTAPLIFVFFFAQRFILESVVVS
                     GLKG"
     gene            complement(1459..>2105)
                     /locus_tag="PLG52_12230"
     CDS             complement(1459..>2105)
                     /locus_tag="PLG52_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNB42262.1"
                     /translation="NTGTGIINQIIRGITEAFPLFGYISRGIIYIQEVSAAFFLGLNT
                     GKYNLMMKVIEAGFPAENRVPLWVQSPLWTKMSVVLLMVWGCGAMMLIFLAGLNNIPK
                     ELHEAAEVDGASPWQRFWKISFPLLTPYIFYNLVVGMISALQIFEPIFVLYRDNQPVV
                     SSTISMVYYLWQKSFSHFEIGYGSAISWVILVIIMIVTVIQFRLQDRWVTYDIY"
BASE COUNT          613 a          497 c          526 g          469 t
ORIGIN      
        1 acagcgtgga gtcgctcacg cgagtgcggc acagttccaa gtccatgatg tgaatcgctg
       61 gcggtatctc cgtgaccagg aaaatgcttg accgttgccg ccactccttg agattgaatc
      121 ccttcgatca tggcagccgc cagttccccc acaagagctg ggtcttcgcc aaacgaacgc
      181 acgcccacaa cgggattgtt cgggttgatg ttaacatcgg cactaggtgc gtaattaaca
      241 ttgacaccaa tcgccgctaa ctcacgccca agcacttcgc ctgctttcct tgcaagttcg
      301 ggtgagcgtg ttgcaccgag agccatgttg ccgggcaagg gggtaccatc gccgaacgcc
      361 atgagttgac caccctcttg gtcagtggca atcaacaaaa gcggcagacc caactcacgc
      421 gcgaggtctt gcagcgatcg gtttagcttt ttgatctgcg cgggcgactc tatattaaat
      481 ggacgaaaga gcgtcacacc gctgggatga taatttttaa atgcctgaat aatctcaggc
      541 gaaagacttt ccttgccacg aaatgccaac aaaagttttt gaccgatctg atgttcgaga
      601 tccacgttaa cctttcaagc cagaaacgac gacactctcg agaatgaatc gttgagcgaa
      661 aaagaatacg aaaataagtg gagccgtggt cagcacggcg gcggtcatga tggtgggcca
      721 aggctgaact gtttggttga tgttgtacag agtggaaaca tagacaggca gtgtgtaaag
      781 atcaacagtc tgcaagtaca tgagtggtgt aagcaggcta ttccacaaac ctacaaagag
      841 aaaggtggca acagttgcca tcggtgcttt gactaagggc aaaatgatct cccaccaaat
      901 ttgaatatga tttgctccat ccacgaatgc ggattcatct aactcttttg gaatctgtga
      961 catggactgc cgcagtaaaa atatcatcgc cgcgccgccc gcgaaataac cgggaatgat
     1021 gagtggattg aaagtaccaa tccagtctaa cttattaaag aagacatatt gcggcacaat
     1081 gagcgcttgc ggcgggatca tcatggtaga aagcattaga aaaaaaacta cattacgccc
     1141 gggccagtcc acgcggctga aggcataggc aaccgccgca atggaaagaa gtgtacccac
     1201 caaagcaagg gtaacgtaaa agaccgagtt gaaataggat cgcagaaaat ccggattttt
     1261 gaagatcgcg gtgtaagctt ccactgtcgg ctcgcgcgga atccagacca cgccgggcga
     1321 gttggtttcc tctactgttt tgaatgagga ggagatcatc caaaacagtg ggaaactcat
     1381 caagaccgtc gccccggtca acaaggtgta aagcacaatg cttttgagaa tgggaacata
     1441 ttttttcatt gtgctctcct aataaatatc gtaggtcacc cagcggtctt gcaagcggaa
     1501 ctggataact gtaacgatca taataatgac aagaatgacc catgaaatgg cagagccata
     1561 cccgatttcg aaatgactaa aactcttttg ccaaaggtaa taaaccatgg aaatggttga
     1621 agaaaccacc ggttggttat cacgatagag gacgaagatc ggttcaaaga tctgcaaggc
     1681 actgatcatg cctacaacga ggttgtaaaa aatatatggg gtgagaagcg ggaaggaaat
     1741 cttccagaaa cgctgccaag ggctagctcc gtctacttcg gcggcttcat gcaattcctt
     1801 gggaatattg ttcaatcccg cgagaaagat caacatcatg gcgccgcagc cccagaccat
     1861 gagcaagaca accgacattt ttgtccacaa cgggctttgc acccaaagtg gaacgcggtt
     1921 ctcagccgga aagcctgctt cgataacctt catcatcagg ttgtatttgc cagtattcaa
     1981 gccgaggaag aatgcggcag aaacttcttg tatatagata atgccacggc tgatatagcc
     2041 aaagagcgga aaagcttcgg taattccgcg aataatttga ttaataatgc ccgtgcctgt
     2101 gttca
//
LOCUS       DAOQNJ010000504         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_44095_length_2100_cov_10.987775, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000504 DAOQNJ010000000
VERSION     DAOQNJ010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44095_length_2100_cov_10.987775"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLG52_12235"
     CDS             complement(<1..439)
                     /locus_tag="PLG52_12235"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNB42263.1"
                     /translation="MFFLSPTYLMYMIPAFILMALTSWYVKSAYAKWSRVAAGSRFSG
                     YEATRRLIASSGLQGVKIQGTPGEMTDHYDPRDKTVYLSNGVANSASVAALAIAAHEL
                     GHAQQDAEEYFPLKFRGVLVPLVNIGSNLGWILIMIGLFLQLTQ"
     gene            complement(466..1734)
                     /locus_tag="PLG52_12240"
     CDS             complement(466..1734)
                     /locus_tag="PLG52_12240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNB42264.1"
                     /translation="MNKTLKAVLLILAVAVVGLGSFAGGFITGHLLPFNNSSAFSFGE
                     APTAEPAPTASPEQQSSTPEDMQTLFAPFWEAWNLVHQNYVDQPVDDVALMRGAISGM
                     MQALGDQHSSYMDPEDFKQANQSLEGSYEGIGAYVDTTSDYLTITSPIPGSPAEEAGL
                     LPGDKIVAIDGEDMTGIDGELVRQKVLGPAGSTVVLTIMRDGEDKPLDFSIVRAQITI
                     ASATGKMLDGDIAYFQVTTFGSNTMPELEAALAEVMPQKPKAIILDLRNNGGGYLQTA
                     VEVASQFMGEGVVLYEQYGDGTRNTFEVIPGGKATDTSIPMIVLINEGSASASEIVAG
                     ALQDTGRAKLLGTVSYGKGSVQNWIPLSNDNGAVRITIAKWLTPNERTIHKIGLTPDY
                     PVDLTPEDRQADKDPQLDEAVRLLLEMLAK"
     gene            complement(1836..>2100)
                     /locus_tag="PLG52_12245"
     CDS             complement(1836..>2100)
                     /locus_tag="PLG52_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42265.1"
                     /translation="SSSPFWRATMEMSKTAIAALIFILMIVGVNVVMYGIVRGVMRGG
                     KDDPLLKMMKAMNPAQQKKENEMEELRRTVDELNKGKTGGDSE"
BASE COUNT          495 a          507 c          564 g          534 t
ORIGIN      
        1 gctgtgtcaa ttgcaggaac aagccgatca tgatgaggat ccagccgaga ttggagccga
       61 tattgacaag cggtaccagc acaccgcgga acttgagcgg aaaatattct tctgcatctt
      121 gctgggcatg accaagctca tgtgcggcaa ttgccaaagc cgctacagaa gcactgttcg
      181 cgacgccatt cgaaagatac acggtcttat cgcgcgggtc gtagtggtcg gtcatttcgc
      241 cgggagtgcc ttggattttc acgccttgca aaccgctgga ggcgattagg cgacgtgtgg
      301 cttcgtaccc gctgaagcgg ctgcctgctg cgactcggct ccattttgca tatgctgatt
      361 tgacatacca tgatgtaagc gccatcaaaa tgaaggcggg gatcatgtac attaagtaag
      421 tgggactcaa gaaaaacatt cagttctcct ttctagttga ttaagctatt tggcgagcat
      481 ctccaagagg aggcgtacag cttcatccaa ttgtgggtct ttatctgcct gacggtcttc
      541 gggcgtaagg tctacagggt aatctggggt aagcccgatt ttatggatgg tgcgttcgtt
      601 cggagtgagc cattttgcga tggtaatgcg gacggctcca ttgtcgtttg aaagcggaat
      661 ccagttttgt acggagcctt ttccatagga gacggttcca agcaacttgg cgcgaccggt
      721 atcttgcaat gcgccagcta cgatctcaga agccgaagca gacccttcgt tgatcaatac
      781 gatcatgggg atgctggtat cggtcgcttt gccgccaggg atcacttcaa aggtgttgcg
      841 agtgccatct ccatattgtt cgtagagcac aacgccttcg cccataaatt gcgaggcaac
      901 ttccacggcg gtttgtaaat aaccaccacc attgttacgc aggtcgagaa tgatagcttt
      961 cggtttctgc ggcataactt cagcgagggc ggcttccaac tcgggcatgg tgtttgagcc
     1021 gaaggtcgtc acttggaagt aagcgatatc accatcgagc attttgccag tggcagaggc
     1081 aatggtgatc tgggcgcgga cgatggagaa gtctagtggt ttgtcttcgc catcacgcat
     1141 aatcgtcagc accacggtgg aaccggcagg tccgagcact ttctggcgga cgagttcccc
     1201 gtcgataccg gtcatatctt cgccatcaat ggcaacgatc ttatcgccgg gtaataaacc
     1261 tgcttcttca gccggagagc cgggaatggg gctggtgatg gtcaaataat cactggttgt
     1321 atccacatag gcgccaatgc cttcatagga accttccaag ctttgattgg cttgcttgaa
     1381 atcttcaggg tccatgtagg acgaatgttg gtcgcccaat gcctgcatca tgccgctgat
     1441 ggcaccgcgc atgagagcaa catcgtctac gggttggtcc acgtaatttt ggtggacgag
     1501 gttccacgct tcccagaagg gagcgaagag agtttgcata tcttcagggg ttgaagactg
     1561 ttgttctggc gatgccgtgg gggcaggctc tgcggtggga gcttcgccaa agctgaaggc
     1621 tgaactgttg ttaaaaggca aaaggtgccc ggtaataaag ccaccggcaa aagacccaag
     1681 cccaacgacg gcgacagcca aaataagcag tactgctttt aaagttttat tcacaagatc
     1741 ctccaatagc tagcatacgt agaaagatac caattggtta ttaaagattg ctgaatgaat
     1801 tattcgattt gcgtaagaat cgaacggtta accttttact cagaatcccc gcctgttttg
     1861 cccttgttca attcatcgac tgtgcggcgt aattcttcca tttcgttctc ttttttctgc
     1921 tgggcggggt tcatggcttt catcattttg agcaggggat cgtctttgcc gccgcgcatc
     1981 accccgcgga cgatgccata catcactaca ttaaccccca cgatcatcag aataaagatc
     2041 aaagcggcaa tggcagtttt actcatttcc atggtggctc tccaaaatgg ggatgaactg
//
LOCUS       DAOQNJ010000505         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_44386_length_2093_cov_7.855250, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000505 DAOQNJ010000000
VERSION     DAOQNJ010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44386_length_2093_cov_7.855250"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLG52_12250"
     CDS             complement(<1..343)
                     /locus_tag="PLG52_12250"
                     /inference="COORDINATES: protein motif:HMM:NF040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HNB42266.1"
                     /translation="MRKILLLVLALLLVACSAASEYDQNIKKWQDAEIAHYRFDLFIG
                     CFCPFRDVMPLTIEVQNGKVVSMTQADGLVVATDDPSYELFMSVASVDALFARLDSSL
                     NGDADEVLVTYD"
     gene            complement(441..1778)
                     /locus_tag="PLG52_12255"
     CDS             complement(441..1778)
                     /locus_tag="PLG52_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNB42267.1"
                     /translation="MKNYTTLILAGLVILLAAVLIGVMVYIQNQPAEVRAVQPLVEIK
                     AMEPDSSKWGINFPNQYDSFVKTETNNIDTTYAGSSQFSWLDRDPRQVILFGGYPFSK
                     DYNDDRGHANALKDVRETGRLNLDDSNPKHTPATCYSCKSSDNPGLWAELGMEAYDAM
                     SFNEMTPNINNSIGCSNCHEAGTMRLIATNPALIEALERQGKDWETYSRQEMRSLVCA
                     NCHVEYYFKGDGKYLTFPWDNGTKVEEIISYYEEAGFKDWAYPETDSPMLKAQHPEYE
                     FFTAGSTHYEAGVSCADCHMPYVRDGASKYSSHDVHSPLLNPEEACGQCHTDTDYVIG
                     RVNDIQKQVAETKIAAEDAIIDAMTAIKAATAAGNADPALLDEAKKLHREAQFMWDFI
                     SAENSTGFHNPEYALEVLSKSIDTARRAQMLAAQSVNDSTLLTTGTYYGQTNP"
     gene            complement(1791..>2093)
                     /locus_tag="PLG52_12260"
     CDS             complement(1791..>2093)
                     /locus_tag="PLG52_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42268.1"
                     /translation="DCHLPHDNFVSYYMEKGRQGAKDTYAFVTGNIPVAIRAEEKTKE
                     IVQENCIHCHEDTVDTMLAGAQPFDRYCWECHRSVAHGERGATGAPYQDSVIYPTK"
BASE COUNT          528 a          525 c          502 g          538 t
ORIGIN      
        1 tatcatacgt gacgagtact tcgtctgcat cgccattcaa gctggaatct aatctggcaa
       61 aaagagcatc cacactggca acggacatga acaattcata cgaggggtca tcggttgcaa
      121 caaccaaacc atctgcctga gtcatcgaaa ccactttccc attttgtact tcgattgtca
      181 acggcatcac atctcgaaac ggacagaaac agccaatgaa caggtcgaaa cgataatgag
      241 caatctctgc atcctgccat ttctttatat tctggtcata ctcagaagcg gcagaacagg
      301 caaccagtaa aagcgcaaga acgagcaata aaattttcct catgtaaaac tccttgttta
      361 gagaacgaag attatcccag aaatgttccc aaataaaaaa cgccctgcca gtttggcagg
      421 gcgtagaccg ttaatgtatt ttatgggttg gtttgcccgt aatatgtacc agtggtcagc
      481 aaggtcgagt cattgacaga ctgagccgca agcatttgag cacggcgtgc agtatcaatg
      541 gattttgaga gcacttcgag cgcatactca gggttgtgga aaccggtcga gttttcagca
      601 gagatgaagt cccacatgaa ctgggcttcg cggtgcagtt tcttggcttc gtcgagtaga
      661 gctgggtcgg cgttgccagc cgcggttgca gctttgatag cagtcattgc atcaatgatg
      721 gcatcttcgg cagcgatttt ggtctcagcc acctgcttct gaatatcgtt cacacgaccg
      781 atcacatagt ctgtatcggt atgacactga ccacaagctt cttccgggtt gagcaaagga
      841 ctgtggacat catgcgaaga atacttgctt gcaccatcgc ggacgtaggg catgtggcag
      901 tcggcacagg agacaccggc ttcatagtga gtgcttccgg cagtgaagaa ttcatattcg
      961 gggtgttgcg ctttgagcat cgggctatct gtttcaggat atgcccaatc tttgaaacca
     1021 gcttcttcgt agtaagaaat aatctcttcg accttggtgc cgttatccca cgggaaggtg
     1081 agatattttc catcgccttt gaaataatat tccacatggc agttcgcgca caccagcgag
     1141 cgcatttcct ggcgcgagta ggtttcccaa tccttgccct ggcgttcgag ggcttcgatc
     1201 agcgccgggt tggtggcgat tagacgcatc gtcccagctt cgtggcagtt cgagcagcca
     1261 atggaattgt tgatgttcgg cgtcatctcg ttgaaagaca tcgcatcata ggcttccatg
     1321 ccaagttctg cccacaaacc ggggttatca gaagatttac aggaatagca tgtagcaggt
     1381 gtatgtttgg ggttcgagtc atccaaattc aaacgcccgg tttcacgcac atctttcaac
     1441 gcattggcat gaccgcgatc atcgttgtaa tctttgctga acggatagcc accaaacaga
     1501 atcacctgac gggggtcacg gtcgagccat gaaaactgtg aagaaccggc ataggtcgta
     1561 tcgatattgt tcgtttcggt cttcacaaat gagtcatatt gattgggaaa gttaattccc
     1621 catttggaag agtccggctc cattgcctta atctccacca acggctgcac ggcgcgcact
     1681 tccgcaggct gattttggat gtagaccatt acgccaatca ataccgcggc aagcaagatc
     1741 accaacccgg caagtattaa agttgtatag tttttcattt tgtattctcc ttacttcgtc
     1801 ggataaatta cagaatcttg atacggcgca cctgtcgccc cacgctcccc atgcgcaaca
     1861 ctgcggtgac actcccagca ataccggtcg aacggctgtg cgcccgccag catggtatct
     1921 acagtatctt catggcaatg gatgcaattc tcctgcacga tctctttcgt cttctcttcc
     1981 gcacggatgg caaccggaat attccccgtg acaaaggcat atgtatcttt cgcaccctgg
     2041 cgtccttttt ccatgtaata cgaaacgaaa ttatcgtgag gcaaatggca gtc
//
LOCUS       DAOQNJ010000506         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_44636_length_2087_cov_7.191437, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000506 DAOQNJ010000000
VERSION     DAOQNJ010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44636_length_2087_cov_7.191437"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..763
                     /locus_tag="PLG52_12265"
     CDS             227..763
                     /locus_tag="PLG52_12265"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNB42269.1"
                     /translation="MFKRNNTPAEPVQPVQAVERITSVLGSGVIWHGSINGSGGVRIE
                     GAFEGEIALRGMLVVGETGRVTCQNVRANTVIVAGAVRGNITTQKLEIRATGRVWGDV
                     VTTAFVTEEGAFLRGQIRMEESVDLNLEPAPEATPSETAQAEVIAQTPIQIPEPAPVP
                     ASTPVSTQRLPRKKKTEE"
     gene            806..2017
                     /locus_tag="PLG52_12270"
     CDS             806..2017
                     /locus_tag="PLG52_12270"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNB42270.1"
                     /translation="MKYTSLSIQTQREFPNNARTQGFGWLVRAGYMTRENQLLPLGEK
                     AIEKLKTQSTEVTFLSSLELPLISNADETFFESAIGSQEIIYCQSCGYIARRELAQTR
                     KTPFAQEEPLPIEKIATPDTNTIESLANLLNIPKEKTAKALMYTRISDGRFVFIVVRG
                     DMQLSEPKLKALVGDVRLATAEEIIGVGAVAGYASPVGLKKEALIVVDDLIQQSSNLV
                     AGANEAGFHLLNTNSGRDYTPEIVADVTLAREGDACAQCGEALSVRRAEILAIRGEFF
                     FDEILLALAETYHDDKGLTLPTFASAFDVYLMHVPGRTMDTKAKAEEIYNALQNAGIS
                     VLFDDRDERAGVKFNDADLIGCPVRLTVGEKGLQNGMVELKRRASSENGTVSVDSLLN
                     SKDINLLISKN"
BASE COUNT          549 a          521 c          530 g          487 t
ORIGIN      
        1 gataaatctc ccatgcgcga gaatgtcacc attgaagatg ttggcgatgc cgcggtcttc
       61 ctggcttcgg atctgtcgaa gcgcatcaca ggcgaagttc tatacgtaga ctcgggcttc
      121 aacatcatgg gtgtgcaaat ggtggaagaa aaaggcgaat aagaaagcta atttcaaggg
      181 cgagcatgat gctcgccctt tttgttttaa cggtaaaatt accttcatgt ttaagagaaa
      241 caatactccc gcagaacccg tacagcccgt ccaagccgta gagcgcatca cttcggtgct
      301 gggctctggc gtaatctggc atgggtccat caatgggtcc ggcggtgtac gcattgaagg
      361 tgcattcgaa ggcgaaatcg ccctacgcgg tatgctcgtg gtcggcgaga ccgggcgtgt
      421 gacctgccag aacgtgcgcg ccaacacggt cattgtggca ggtgcggtac gtggaaacat
      481 caccacccaa aagcttgaaa tccgtgcaac cgggcgtgtg tggggtgacg tggtcaccac
      541 cgcctttgtg acggaagagg gcgcgttcct gcgcggtcaa attcggatgg aagaatcggt
      601 cgaccttaat ctcgagcctg ctcctgaagc cacgccttcg gaaaccgctc aagctgaagt
      661 catcgcgcag acaccaatcc aaatccctga gccggcacct gtcccggcat ccactcctgt
      721 tagcacacaa cgcttgcccc gcaagaagaa aacagaagaa taatctcact ttccccctct
      781 cctaaaaaga gaggggaatt tgtatatgaa atacacctcc ctttccattc aaactcaacg
      841 tgaatttccc aataatgccc gcacacaggg atttggctgg ctcgtccgcg ccgggtatat
      901 gacccgtgaa aatcagcttt tgcccttggg tgaaaaagcc attgaaaaac tgaaaacaca
      961 gtcaacagaa gtaacgttct tgagttcgct cgaattaccc ttgatctcca atgcagacga
     1021 aacttttttt gagtcagcga ttggctcaca agagatcatc tattgccaat catgcggata
     1081 tatcgcccgt agagaacttg cccaaactcg caaaactcca tttgcgcaag aagaacctct
     1141 gcctattgaa aagatcgcca cacccgatac gaataccatc gagtcgcttg caaatctgct
     1201 gaacattccc aaagaaaaaa cagccaaggc gttgatgtac acccgcatat ccgatggcag
     1261 atttgttttc atcgtcgtgc gcggtgacat gcaattgagc gaaccgaagt tgaaggcttt
     1321 ggttggagac gtccgcttgg cgacggcgga agaaattatc ggcgtgggag ccgttgcggg
     1381 gtatgcatct ccagttgggt taaagaaaga ggcgttgatt gttgtcgatg acctcattca
     1441 gcaatcgtcg aatctcgtgg cgggtgccaa tgaggctggc ttccatttgc tcaacaccaa
     1501 cagcggacgt gactatacac cagagatcgt ggcggatgtg acccttgcac gcgaaggcga
     1561 tgcctgtgct cagtgcggcg aggcgttatc agttcgtcgg gctgagatcc ttgccatccg
     1621 tggagagttt ttctttgacg agattttgct tgcccttgct gagacctatc acgatgacaa
     1681 aggactgaca ctgcctacat ttgcttctgc attcgacgtg tatctgatgc acgtccccgg
     1741 taggacgatg gatacgaaag ctaaagctga agaaatctat aacgccctac aaaatgctgg
     1801 catctcggtc ttgtttgatg accgtgacga gcgtgctgga gtcaaattca acgatgccga
     1861 cctgatcgga tgtcctgtgc gtctcaccgt cggtgaaaaa gggcttcaaa acggaatggt
     1921 agaattgaaa cgacgcgcat ccagtgaaaa cggcacggta tcagttgata gtctcttgaa
     1981 ttcaaaggat attaatttac tcataagcaa aaattaagag gtgacaatat gaacaataaa
     2041 gttccatctt ttgggcagct ctattccact gactacaata tcaagtc
//
LOCUS       DAOQNJ010000507         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_45143_length_2075_cov_9.562376, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000507 DAOQNJ010000000
VERSION     DAOQNJ010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45143_length_2075_cov_9.562376"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1524)
                     /locus_tag="PLG52_12275"
     CDS             complement(85..1524)
                     /locus_tag="PLG52_12275"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNB42271.1"
                     /translation="MKNSVFSIDNLPRLLWGLALLTLPVTSFRWFPGLGEGALVRPLA
                     LYPLAFLLPLLLIQAWRKKIQLTWAGAFVALGAFILFVFFSSAIGSLINPISLRGQVY
                     NGRTIRALLTLFIGIAFFVSAVWMNKNEEDLRFTVRWIFAGLCLDIAWSGLQAVTFYT
                     GLLEKEMVTHWQLAFSMRELVRTNRISGLAYEPAWLAGQIATTFFPFLFAAVLTKYRV
                     TRLSWLEPALLAVTTLVLLATYSRGGLLIILGTSGLMMLLFGRDILKGMWAWFISGFR
                     GRISDLALRIGMVAVLVGIVAGAVLFLGQKNYFRRLWEINAESLSEYIVDINAGARGA
                     YSVGALGAFSEYPLTGVGLGGSGFYIYKNLPDWALTTVPEIAKQLNPSNKLYPNPKNL
                     YVRLLAETGLFGFVLFFAFLFNVLGDMLSLLKRSEPWARFAAVAGVFAWVAIALYNFT
                     QDSLTTPNIWIVPGILVGLATHQLNKEVS"
     gene            complement(1521..>2075)
                     /locus_tag="PLG52_12280"
     CDS             complement(1521..>2075)
                     /locus_tag="PLG52_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42272.1"
                     /translation="LKAWRFWVLGAIIGSAIGVAFYDIAPPPYRAKAIVNVDFNFEEA
                     FPANTDRQDFYYLEREARKMVELAWSDDVLNKLNAPVEELREGKLTLSQPAEAGWHFY
                     ADDQNPQKAEELASAWANAFVEKTQTEIQSGNLNEFIKLEVTQSANLPKQRSVPLANY
                     LLTGSSGFLLLAMFAVLFIKPKAK"
BASE COUNT          616 a          534 c          497 g          428 t
ORIGIN      
        1 cacagcccgt ggttttcatc aggtcgtgaa tgaggttata gtgccagccc gacccagcgc
       61 gcggcatccc aacagaaaga acgatcatga aacttccttg ttcaattgat gtgtagcaag
      121 ccccaccaag atccccggca cgatccagat attcggggtg gtaagcgaat cctgggtgaa
      181 attatacagg gcgatagcca cccacgcaaa tacaccagcc accgccgcaa agcgcgccca
      241 tggctcgctc cgtttgagca gggagagcat atctccaagc acattgaaga gaaatgcaaa
      301 aaagagaacg aagccaaaca acccagtttc cgcaagcaaa cgtacatata aattcttggg
      361 gttcgggtac agtttattag atggattcaa ctgcttcgcg atttctggga cggtggtcaa
      421 cgcccaatcg ggcaaatttt tatagatgta aaacccgctt cctcccaagc cgacgcctgt
      481 taacgggtat tctgaaaacg ctcccaacgc cccaaccgag tacgctccgc gcgccccggc
      541 gttaatatcc acaatgtatt cgctcaaact ctcggcgttg atctcccaca agcggcggaa
      601 gtaattcttc tgcccgagga acaagaccgc accagccaca ataccgacca acaccgcaac
      661 cataccaata cgcaatgcaa ggtcagagat acgtccacga aagccgctga tgaaccaagc
      721 ccacatgcct ttaagaatat cgcgaccaaa gagcaacatc atcaagcccg atgtgccaag
      781 gatgatgagc aagccgccgc gggaataggt agccaaaagc accaatgttg taactgctag
      841 caacgcaggc tccagccacg ataggcgtgt gacgcggtac ttcgtcaaaa cagccgcaaa
      901 gaggaagggg aagaatgtcg tcgcaatttg accggcaagc cacgccggtt catatgccaa
      961 gcccgagata cgattcgtgc gaacgagttc gcgcatcgaa aaagcaagct gccagtgcgt
     1021 gaccatttct ttttcaagca agccagtata aaaggtgacc gcttgcaaac cgctccaggc
     1081 gatatcaaga catagacctg caaaaatcca tctgacggtg aagcgcaggt cttcttcgtt
     1141 tttattcatc catacagcac tgacaaagaa ggcaatgcca ataaacaacg tgagcaaggc
     1201 tcgaatggtg cgaccgttat acacttgccc gcgcaacgaa attgggttga taagcgaacc
     1261 aatcgccgaa gaaaaaaaca caaataggat aaaagcaccc agagcaacaa atgcgcctgc
     1321 ccatgtaagt tgaatctttt tacgccaggc ttgaattaag agcaggggca ataaaaacgc
     1381 caatggatac aatgctagtg gacggacgag cgcaccttcg cccaaaccgg ggaaccaccg
     1441 gaaactggtg acaggcaggg ttagcaaagc cagtccccat aataggcgcg gaaggttatc
     1501 tattgaaaaa acagaattct tcatttggct ttgggtttaa taaacagaac agcaaacatg
     1561 gcgagcaaga gaaatccgct tgagcctgtt agtagataat tagcaagtgg aacactgcgt
     1621 tgtttgggca ggttggcaga ctgggtcact tcaagtttga taaattcgtt cagattgcca
     1681 gattgaattt cggtttgagt tttttcaaca aaggcgttcg cccatgctga agcaagttct
     1741 tctgcttttt gcgggttttg atcatcggca taaaaatgcc agccagcttc agcaggttga
     1801 gaaagggtca gctttccttc acgcagttct tcaacaggag cattcaattt attcaacaca
     1861 tcgtctgacc aggcaagctc caccatcttg cgagcttcac gctcaagata gtaaaagtct
     1921 tgccggtctg tattggctgg aaaagcttct tcgaaattaa agtctacgtt gacaatggct
     1981 ttggcacgat acggcggcgg ggcgatgtca tagaacgcaa cgccaatggc agagccaatg
     2041 atcgcgccca atacccaaaa acgccaggct ttgag
//
LOCUS       DAOQNJ010000508         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_45312_length_2071_cov_10.751984, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000508 DAOQNJ010000000
VERSION     DAOQNJ010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45312_length_2071_cov_10.751984"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLG52_12285"
     CDS             complement(<1..719)
                     /locus_tag="PLG52_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB42273.1"
                     /translation="MQTLNHQFELIRQSATVSLTDRILALKASGKPIISLQVGDPDFG
                     THPAIAEVALKAMQEGQTHYGPSNGTLELRKAIAHKLQRDEGVSYDPNTEILVTNGGI
                     HAYYTAMQSILNPGDDVLIPDPSWATHSNMATMLRGNVIRIPAPAENGFIPHFDSWLK
                     ALTPKTRVIVLNYPSNPTGAYPTREYLQQLQNFAAKNNLWIVSDEVYGSLYYGDKPTS
                     AAAIEGAKERTLLVHSLSKSYA"
     gene            complement(789..>2071)
                     /locus_tag="PLG52_12290"
     CDS             complement(789..>2071)
                     /locus_tag="PLG52_12290"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB42274.1"
                     /translation="PKVICMTDSQTKILSRLLEVTRSLDSEADLESSLRLILSAATEL
                     TASETASLLEYDETALNFFFKYVPWFNQDSQGGSTLRIPLNESIAGWVLLNKSSVTVN
                     DAINDPRHSKKVDEFAGITIHSVLGAPLIVRDVPIGVLEVFNKRSNYTEEDVLIVENL
                     AALAAAAMEKDLLEKKVASSKEEARELDRMKNEFIAITSHELRTPLGLILGHSTFLKE
                     LLGGSYEDQVDVIIRNASRLKEIIESLTSVDNYQSGGALVRSRKVSIARIIEDVIVSF
                     SEMANKKKITIKKALQPGYELWVDVDAGKVAIVLSNIMKNAITFTNEGGEIIIRGEQH
                     PDYVKVSVKDNGIGIPTKDLPHVFDRFYQVEGHLTRRHGGMGLGLSVAKVMVEMHGGR
                     IWADSEEGKGSIFSFILPVRSEPPAPASSSPFSI"
BASE COUNT          545 a          494 c          492 g          540 t
ORIGIN      
        1 gcataggact tggacaagct gtggaccaat aacgtccgct ctttcgcgcc ttcgatggca
       61 gcagcactgg tcggtttatc cccgtaatac aaactgccat agacttcatc actaacaatc
      121 cataaattat tttttgcagc aaagttttga agctgttgca agtattcgcg agtgggatat
      181 gcaccggtcg ggtttgaggg atagttcaga acaatgactc gggttttggg agtaagagct
      241 ttcagccaag agtcgaaatg agggatgaac ccattctcgg cgggggcagg gatgcgaatg
      301 acatttccac gcagcatggt tgccatgttg gaatgagttg cccaggaagg atccggtatc
      361 aagacgtcat cacccggatt caaaatggat tgcatcgccg tatagtatgc gtgaatgccg
      421 ccgttggtga cgagaatttc tgtattagga tcgtagctta cgccttcatc gcgttgaagt
      481 ttgtgcgcga tcgctttgcg aagttcaagc gtgccgttgg aaggtccata atgagtttga
      541 ccttcttgca tggctttaag cgccacttca gcaatggctg gatgcgtgcc aaaatcggga
      601 tcaccgactt gcaagctgat gatgggtttg ccgctggctt tgagcgcgag aatgcggtcg
      661 gtcaatgaga ctgtggcaga ttggcggatc aattcaaatt gatgattaag agtttgcata
      721 gaatgtcctt caaagaatgg gcgtgagtgt atagggaaat aaaaatcccg tcaacggtgt
      781 gacgggactc aaatggaaaa gggagaagag gatgctggcg ctggcggctc gctgcgaaca
      841 ggaagaataa aggaaaagat actccctttg ccctcttcgc tatcagccca aatacgcccg
      901 ccatgcattt caaccatcac cttggcaacg gaaagtccca accccatgcc accgtgtcgg
      961 cgtgtgaggt gaccttccac ctgatagaaa cggtcaaaca cgtgcggcag gtctttggta
     1021 ggaatcccaa ttccgttatc tttcaccgag acttttacat aatccggatg ctgttcacca
     1081 cggatgatga tttctccgcc ttcattcgta aatgtaatag cgttcttcat aatattgctc
     1141 agaacaatgg caactttccc agcatctaca tccacccaca attcataccc aggctgaaga
     1201 gcttttttga tcgtgatctt tttcttattc gccatttcag aaaaagagac gatcacatct
     1261 tcaataattc ttgcaataga aactttgcgc gagcggacca atgccccacc gctctggtag
     1321 ttatccacgc tggttaagct ctcgataatt tctttcaaac gagaggcatt acgaatgatg
     1381 acatccacct ggtcttcata actcccacct aataattctt tcaagaacgt agaatgtcca
     1441 aggatgagac ccaagggcgt gcgcagttca tgtgatgtga tcgcgataaa ctcattcttc
     1501 atccgatcaa gttcacgcgc ctcttctttc gacgaagcga ctttcttctc aagaaggtcc
     1561 ttttccatcg ctgcagccgc cagtgccgcc aagttttcaa cgattagaac atcttcttct
     1621 gtgtagttac tgcgtttatt gaagacttcc aaaaccccaa tcggcacatc gcgaacaatc
     1681 aacggtgccc ctaaaacgga gtggatggtt atgcctgcaa attcatccac ctttttggaa
     1741 tgacgcgggt cattgatcgc atcgttcaca gtcaccgatg atttattcag caaaacccaa
     1801 ccggcaatac tctcattcaa cggaatacgg agcgtacttc caccttgtga atcttgattg
     1861 aaccaaggga catatttgaa aaagaaattt agagccgtct catcatattc caaaagagac
     1921 gcggtttcgc tcgccgtcag ttcggtagcg gcagagagga ttaaacgtaa actggactcc
     1981 aaatccgctt cagagtcgag gctgcgtgtc acttcgagga gacgtgaaag gattttcgtc
     2041 tgtgagtccg tcatacaaat aaccttgggt a
//
LOCUS       DAOQNJ010000509         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_45523_length_2066_cov_9.419194, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000509 DAOQNJ010000000
VERSION     DAOQNJ010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45523_length_2066_cov_9.419194"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLG52_12295"
     CDS             complement(<1..728)
                     /locus_tag="PLG52_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42275.1"
                     /translation="MKNKKSLLLVLLLLVSLACSLTGTPTPETSAPLESIAPTFTPIP
                     SFTPEAQTASAALSSGLTVQSSPVEEAGTEPPYTIKAQVPYLQGSDDARVQSFNAYLK
                     QIVQNEIDGFKTNTLAYASTPPMVNGSSLEIQYSVLGQRGDIWSIQFLVYFYADGAAH
                     PGHYSISVNYDLANSREVTLDELFLPGSDYLTTLADISKAELATRDIGFESFSTGADP
                     LPENYTRWNLSNEGFLVITFDEYQV"
     gene            813..1538
                     /gene="rlmB"
                     /locus_tag="PLG52_12300"
     CDS             813..1538
                     /gene="rlmB"
                     /locus_tag="PLG52_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNB42276.1"
                     /translation="MKEFIYSRNAVHEALLAKRRDIFSIEIAEGAQEKGTLAEIIKIA
                     QQRKIKLFRSPRPKLDKVNQHHQGVVAEVSGYPYADVVDILDKPKGEPPFILILDSLQ
                     DPQNFGTLIRTAEAVGVHGIIIPLARSVEVTPSAVNASSGASEHMLIAKSNLSQAMDA
                     LRDADIWLVGLDQAGAEVEANSRHLKGALGLVVGSEGEGIHELTRKKCDIVLKLPMKG
                     KIESLNAAVAGSVALYLAYLNRK"
     gene            1588..>2066
                     /locus_tag="PLG52_12305"
     CDS             1588..>2066
                     /locus_tag="PLG52_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNB42277.1"
                     /translation="MPQGTYHFPKGFLWGTATASHQVEGNNTNNNWWKWEQEGHTNGS
                     AAIAADWWGGRWREDFDRAAETGQNAHRLSIEWSRVQPTPDRWDEDAIERYRTMLRGL
                     RDRNMTALVTLHHFTDPLWVTERGAWETEEIVPLFEKYVRKMVDSLKEYTNLWVTIN"
BASE COUNT          534 a          462 c          585 g          485 t
ORIGIN      
        1 acttgatatt catcaaaggt gatcacaaga aacccttcgt tcgaaagatt ccagcgtgtg
       61 taattttcag gcaatgggtc tgcaccggta gaaaaacttt cgaagccaat atcgcgtgta
      121 gcaagttcgg ctttcgaaat atcagcaagg gtcgttagat aatcagaacc cggcagaaaa
      181 agttcatcca acgttacctc acggctgttt gccagatcat aattgaccga gatgctgtaa
      241 tgcccagggt gcgctgcgcc atcggcatag aaataaacaa ggaactgaat actccaaata
      301 tcgccacgtt gcccaagtac gctgtattga atttcaagcg agcttccgtt caccatgggc
      361 ggcgtgctgg catacgcaag tgtgttggtc ttgaagccat ctatttcatt ttgaacgatc
      421 tgcttgagat acgcattaaa gctttggaca cgcgcatcat cggagccttg caagtaaggg
      481 acttgggctt tgatggtgta gggcggttca gtccccgcct cttccaccgg cgacgattga
      541 accgtcagcc cagagcttag cgccgctgaa gctgtttgtg cttccggcgt aaacgaaggg
      601 ataggcgtga aggtgggtgc aatactttct aacggagcag aggtttctgg ggttggtgtt
      661 ccggtcagcg agcaggcgag tgaaacaagc aaaagcaata ccagtaaaag agactttttg
      721 tttttcattg gaactccttg gaatgcttgg attctatcgc ttaagtggat ttatgcaggg
      781 acatatcgtt atcaagtaaa atacactttg atatgaaaga attcatctat tcacgcaatg
      841 ccgtccatga agccttgctt gccaaacgca gggacatttt ttcaatagaa attgccgaag
      901 gggcacaaga aaaaggcaca ctggcagaga tcatcaaaat tgcgcaacag agaaagatca
      961 agctattccg gtcgccgcgc cccaagttag acaaggtcaa ccagcaccat caaggtgtgg
     1021 tggcagaggt gagcggctat ccctatgcag atgtggtcga tattctggat aagcccaaag
     1081 gcgaaccgcc tttcatcctc attcttgact cgttgcaaga cccgcagaac ttcggcaccc
     1141 tcatccgcac tgcagaagcg gtgggagtgc atggcattat cattccgctg gcgcgctcag
     1201 tggaggtgac accctctgcg gtgaatgctt cttcaggcgc gagtgagcat atgctcatcg
     1261 ccaagtcgaa tctttcgcag gcaatggatg cgcttcgaga tgcagacatt tggctggtag
     1321 ggttggatca agccggggcg gaggtggagg cgaattcccg tcatctcaaa ggagcattgg
     1381 gattggtcgt cggctcggag ggtgaaggca tccacgaact gacgcgcaag aagtgtgaca
     1441 ttgtgttgaa gttgccgatg aagggcaaaa ttgaatcttt gaatgctgct gtggcagggt
     1501 cggtggcatt gtatttggcg tatttgaata gaaaataatg tgtaggggcg acttgcgagt
     1561 cgcccctacg atactatgga gatcatcatg cctcagggaa cgtatcattt tccaaaaggg
     1621 tttttatggg ggacggcgac tgcttcgcat caggtagaag gcaataacac aaataacaat
     1681 tggtggaagt gggagcagga aggtcacacc aatgggtcgg cggcaatcgc tgccgattgg
     1741 tggggcggac gttggcgcga agatttcgac cgtgcagccg agacgggtca gaatgcacat
     1801 cgtctgtcca ttgagtggag ccgtgtgcag cccacacctg atagatggga tgaagacgcc
     1861 atcgaaagat atcgcaccat gttgcgcggg ctgcgtgatc gcaatatgac cgccttggtg
     1921 acgttacatc acttcaccga cccgctgtgg gtgacggaac gtggcgcatg ggaaaccgaa
     1981 gagatcgtgc cgttgttcga gaaatatgtg cgcaagatgg tggattcttt gaaggaatac
     2041 accaacctat gggtgacgat caacga
//
LOCUS       DAOQNJ010000510         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_46646_length_2038_cov_7.834090, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000510 DAOQNJ010000000
VERSION     DAOQNJ010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46646_length_2038_cov_7.834090"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..751
                     /locus_tag="PLG52_12310"
     CDS             122..751
                     /locus_tag="PLG52_12310"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNB42278.1"
                     /translation="MIPKPDLTYENEIWSAYQFIAGMDEAGRGALAGPVSVGAVILPL
                     NHSHLLSALSGVHDSKQLTPRKRSELVPQIQATALAHGVGFASAEEIDQVGIVPATRL
                     AASRALESLSLYPQYLLTDFRLELPELDIPQTAIVKGDQRSLSIACASILAKTARDEF
                     MQNLNEKYPEYRLDHHKGYGTLFHRKKIETLGHSPIHRKTFQLKNNREA"
     gene            complement(768..1604)
                     /locus_tag="PLG52_12315"
     CDS             complement(768..1604)
                     /locus_tag="PLG52_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB42279.1"
                     /translation="MNPKRFFVLLTLASLLFFGLWLPAGASSSQQESPPTPTPGTDGR
                     IIYVVQAGDNCFRVAAINGITVEQLRQLNSKLDENCTLTEGQELLIGVAAVATATAPA
                     NVAVGTPTITPTPLSGLTEVCVLLFNDLNGDAMRQDTEPAIAGGAVSVTEANGKYSAS
                     LETVIPSDPTVYQGTCFSNVPEGTYNITMAIPDQYNPTMSLSYSLEVKAGDRAIVDFS
                     AQSKDVVVDQTETEGNAGGASTGLVIFGIVLLLGGAGLGYYAWRSNKPESKLAGGGIL
                     KK"
     gene            1662..>2038
                     /locus_tag="PLG52_12320"
     CDS             1662..>2038
                     /locus_tag="PLG52_12320"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the hydrolytic cleavage of hydantoin with
                     aromatic side chains at the 5'position; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNB42280.1"
                     /translation="MKTLIKNGTLITASDTFNADILIEDEKISRIGLNLEADSDHQVD
                     ATGKLIMPGGVDPHVHLDLPMFDTVSSDDHYTGHKAAAFGGTTTAMDFVVLENEGFEH
                     SVDLWMKKAEKAAIDYSFHMNLTR"
BASE COUNT          559 a          529 c          440 g          510 t
ORIGIN      
        1 attcgtgaaa tccctattca ttggtatttt gacgcaggca gtaaagtaag tgccgttcgc
       61 gatgctctac gtatgatcag cgatatcttc cgtattcacc tgaatgccct tcgtggcacg
      121 tatgattcca aagcctgatt taacctacga aaatgagatt tggtctgcgt atcaattcat
      181 agcaggcatg gatgaagccg gacgcggcgc actggctgga ccggtctcag ttggggcagt
      241 gattctccct ctcaatcatt cgcatcttct cagcgccttg agcggagttc acgactctaa
      301 gcaactcaca ccccgaaaac gctccgaact cgttccacaa attcaagcaa ctgccctcgc
      361 ccacggagtc ggattcgcct ccgctgaaga aatagatcaa gtgggaattg tgccagctac
      421 acgtctagct gcgagccgtg cattggaatc tttatctctt tatccgcagt acttactaac
      481 ggatttccgt cttgagctcc ctgagttaga cattccgcaa actgccattg tcaaaggaga
      541 tcaacgctca ttaagtattg cctgcgcatc cattctggcg aaaactgccc gcgatgagtt
      601 catgcaaaat ttgaatgaga aatacccaga atatcgcttg gaccatcaca aaggctatgg
      661 gacgctcttt catcgaaaga agatcgaaac gctcgggcat tctcccattc accgaaaaac
      721 ttttcagtta aaaaacaacc gcgaggctta agcctcgcgg tcttcattta ttttttcaag
      781 atgccgccac cagctaattt actttctggc ttattcgagc gccaggcata atacccaagc
      841 ccagccccgc caagtaataa aacaataccg aagatgacaa gcccagtgga tgcaccacct
      901 gcatttcctt ctgtctcagt ctggtctacc actacatctt tcgattgagc gctgaaatct
      961 acaatagcac gatcaccagc tttcacttct agagaatagc tcaacgacat cgttgggtta
     1021 tattggtcag ggatagccat ggtgatgtta tatgtccctt caggaacatt tgaaaaacaa
     1081 gtaccctgat ataccgttgg gtctgatgga atgactgttt ctaatgaagc agaatatttt
     1141 ccattggctt cggtcacact tactgcgcca ccagcaatag cgggttctgt atcttggcgc
     1201 atagcgtcac cgttaagatc attgaacaaa agcacacaca cttctgttaa accagaaagc
     1261 ggagttggag taatggttgg cgtccccact gctacattcg caggcgctgt tgccgtcgcc
     1321 acagccgcaa caccgatcaa taattcctgt ccctcagtca acgtacaatt ttcatccaac
     1381 tttgaattca actgacgaag ttgttccacc gtgatgccat tgatcgcagc cacgcggaaa
     1441 caattatcgc ctgcttgaac aacataaata atacgcccat ccgtgccagg ggttggtgta
     1501 ggaggagatt cttgctggct cgatgacgcg cccgcaggca accacagccc gaagaacagc
     1561 aaactagcca gggtcaacaa gacgaaaaaa cgctttggat tcattgaaga aaattataac
     1621 agattcgatt tggtataaaa taaagctgaa atgaggcttg catgaagact ctaattaaga
     1681 acgggacctt aatcaccgca tccgacacat tcaacgcgga tattttgatc gaagatgaga
     1741 aaatctcgcg catcggactt aatctcgaag ctgattctga ccatcaggtg gatgccacag
     1801 gtaagctgat catgcccggt ggcgtagacc ctcacgttca cctcgacctg ccgatgttcg
     1861 atactgtttc ttccgatgac cattacaccg gtcacaaagc cgccgccttt ggtgggacaa
     1921 ccactgccat ggactttgtg gtgctagaaa atgaaggctt tgaacattcc gtcgatttat
     1981 ggatgaagaa agctgaaaaa gcagccatcg actattcctt tcacatgaac ctcacccg
//
LOCUS       DAOQNJ010000511         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_46807_length_2034_cov_6.946943, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000511 DAOQNJ010000000
VERSION     DAOQNJ010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46807_length_2034_cov_6.946943"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLG52_12325"
     CDS             <1..207
                     /locus_tag="PLG52_12325"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNB42281.1"
                     /translation="ALHAAIQFGFDQMKLHRIEALVYPENTRSLHLLNKLGFKQEGTL
                     RDYFYLDGKFYDHIVLSLLKTSEI"
     gene            complement(187..1353)
                     /locus_tag="PLG52_12330"
     CDS             complement(187..1353)
                     /locus_tag="PLG52_12330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB42282.1"
                     /translation="MKIALITNSRIPSLTANSIQAMKVAQALIQLGHDIKMFAPKESP
                     ITNHQSLLTHYGLRLAPELELLPSISRLKRLDFIVHAQWAAKKFGADLIYTWLPQSAV
                     LGAWMGYPVVLEMHADNSGRLGVWWMRQFWKQKTPKVMTVTTSALRKALERSTNFRFP
                     NDALLLAPNGVELEKYAGLPSPTEARRQLNLKEGLTVGFTGHIYPGRGADLLFELARQ
                     MPAVNFLWVGGTPALVDFWKNKLTEAQMTNVTMTGFVKHEVIPLYQAAADVLLMPYSR
                     SIEASSGQDIAEVINPMKMFEYMASGRGIVSADLPSIREVLNVGNAVLVEPVEEGNRE
                     LGIGNWKMAIEKLLVDEPHRLALGKQARLDVEQLTWVKREERVMELLESNFRGL"
     gene            complement(1331..>2034)
                     /locus_tag="PLG52_12335"
     CDS             complement(1331..>2034)
                     /locus_tag="PLG52_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNB42283.1"
                     /translation="QVQIHKALGSGWWRHSFSTIDSWREIFRFSISSNVSATIIKIFR
                     ESEVLWVSFFLDTTAVGYYRVAYNLVYFLAIPADPLIHVTFPEISRLVTEKAWNKLKS
                     FLKKITAFSFAYNILLGAAYIFLGRWVILIYSGEKFVNAYPALVALTIGLVFNYILFW
                     NRPLLLAQGLPEFPIYITLIAGITKLALSFWLVPTYGIVAAGALLSFYYITSVGIMAL
                     RGVRQLQHNENRTHH"
BASE COUNT          562 a          539 c          454 g          479 t
ORIGIN      
        1 gcactgcatg ctgccatcca attcggattc gaccaaatga aactacatcg catcgaagca
       61 ttggtttatc ccgaaaatac tcgctctctt cacttgctaa acaaattggg tttcaaacaa
      121 gaaggcacct tgcgcgatta tttttatctt gatggcaagt tttatgacca catagtattg
      181 tctctgctaa agacctcgga aatttgattc taacaattcc atcacccgct cttcgcgctt
      241 cacccacgtc aactgctcca catccaagcg ggcttgtttc cccaacgcca atcgatgcgg
      301 ctcatccaca agcaacttct caatcgccat cttccaattc ccaattccca attccctatt
      361 cccctcttcc accggctcca ccaacactgc attgcccaca ttcaacacct cccgtatcga
      421 aggcaagtcc gcagaaacga tcccccgccc cgacgccata tactcgaaca tcttcatcgg
      481 gttaatgacc tcggcaatgt cctgtccact cgaagcctcg atcgaacgcg aatacggcat
      541 cagcaacaca tccgccgccg cctgatacag cgggatcact tcatgcttca cgaagccggt
      601 cattgtcaca ttggtcatct gcgcttcggt taatttattt ttccaaaaat ccaccaacgc
      661 aggcgtgccg cccacccaca aaaaattcac cgcaggcatt tgacgcgcga gttcaaataa
      721 taagtccgcg ccacgccctg ggtaaatatg tcccgtgaag ccaaccgtga gtccctcttt
      781 caaattcaat tgacgacgcg cttcggtagg acttggcaaa cccgcatact tttccaactc
      841 gacgccgttc ggtgcgagca gcaacgcatc attcgggaac cggaagttgg tcgagcgctc
      901 caaagctttt ctcaacgctg atgtggtgac cgtcatcacc ttgggcgtct tttgtttcca
      961 gaactgtctc atccaccaca caccgagccg accggaatta tccgcatgca tttcgagcac
     1021 aacgggatag cccatccacg cgcccaacac cgcggattgg ggcagccagg tgtagatcaa
     1081 gtccgcgcca aatttcttgg cagcccactg ggcatggacg ataaagtcga ggcgtttgag
     1141 tctgctgatg gatgggagca attcaagttc gggagcgagg cgtaatccgt aatgggtaag
     1201 tagtgattgg tgattagtaa ttggagattc tttgggggcg aacatcttga tgtcatgtcc
     1261 caattgaatc aaagcctgag cgaccttcat cgcttggatc gagttcgcag ttagagatgg
     1321 aattcgtgaa ttagtgatga gtgcgatttt cattgtgttg aagttgcctt actccgcgca
     1381 atgccatgat gccaacggaa gtaatgtaat aaaatgaaag caatgcgcct gcagcaacaa
     1441 tgccataggt tggcaccagc cagaacgaaa gcgcaagttt cgtaatgcca gcaatcagag
     1501 taatgtaaat tgggaattca ggtaagcctt gagccagcaa gagcggacga ttccaaaaga
     1561 ggatgtagtt gaacacaaga ccaatcgtca gcgcgacaag cgctgggtac gcattgacga
     1621 atttttcacc agagtagatg agaataaccc aacgtcctaa aaagatatag gctgcaccga
     1681 gcaaaatgtt atatgcaaac gagaaggctg tgatcttttt gagaaagctt ttgagtttgt
     1741 tccatgcctt ttcggtgacc aaacgactga tctctgggaa ggtcacatga ataagcgggt
     1801 cagcagggat agcaaggaaa taaactaggt tatatgccac acgataatat ccaacggctg
     1861 tggtatcgag aaaaaagcta acccaaagca cttcactctc gcgaaagatc ttaatgatgg
     1921 tagcactgac attggaactg atggaaaagc gaaaaatctc acgccacgaa tcaatagttg
     1981 aaaaagagtg ccgccaccat ccactgccca aggctttatg aatttgcact tgcg
//
LOCUS       DAOQNJ010000512         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_47060_length_2028_cov_7.821085, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000512 DAOQNJ010000000
VERSION     DAOQNJ010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47060_length_2028_cov_7.821085"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLG52_12340"
     CDS             <1..287
                     /locus_tag="PLG52_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB42284.1"
                     /translation="SGGQQQRVAIARSLINDPVMVIADEPTGNLDTRSGKEIMNLLHT
                     LHEQGTTIVMVTHDPKTAIHTQRVITLVDGLVDNITHNKKYVEEAAHEAV"
     gene            274..1500
                     /locus_tag="PLG52_12345"
     CDS             274..1500
                     /locus_tag="PLG52_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB42285.1"
                     /translation="MKPSEALNIAWDAILKNKIRSFLTMLGIIIGVSAVIIMVAISAG
                     TEASIQEQITSLGSNLVFVQSSMMRGGPGQAPEGGLVYDDAAAIETGVDGVTAVVVEQ
                     NSTETVKAGDITLEDVEILGTTIGFPSVRDMEIADGRYFNEQENERKSKVAVLGYTVA
                     QELFGDTNPVGQYIKIGNTKVAVIGVFKEKGLVGNTDFDSRIYLPIKLVFQKFTPSMF
                     ARFMGDSVRMIYVEVDPDANQDELITQIELLLAKRHDVSLDSADFTVTTQQDIISTQE
                     STTSAFRSLLAWVAGVSLIVGGIGIMNIMLVSVTERTREIGIRQSIGATPEDIRLQFL
                     TEALLLSIVGGLIGVIVGVGGAYIFGSLSDMRTVISLPSILLAFGSSAIVGAFFGYYP
                     ANQASKLDPIEALRYE"
     gene            1625..>2028
                     /locus_tag="PLG52_12350"
     CDS             1625..>2028
                     /locus_tag="PLG52_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42286.1"
                     /translation="MSTITKLAIGTLKLEGTENAITPEQASDLLPLWQVYLSLVDSDT
                     AAQAEIDGLVDQISTTLTDSQNQAIDDMQLSQSDMMSVMQEMGISMGNRPQSASDSEQ
                     SGSSGGFPGGGDGFAPPDGGGMPGGGMPSGDMP"
BASE COUNT          541 a          504 c          491 g          492 t
ORIGIN      
        1 tttctggcgg tcagcaacag cgtgtggcaa ttgcgcgttc actgatcaat gacccggtca
       61 tggtcattgc cgatgaaccg acaggaaatc tcgacacacg ttcaggcaaa gagatcatga
      121 atcttctgca tactttgcat gagcaaggca caactattgt catggttacg catgacccta
      181 aaactgccat ccatacccag cgcgtgatca ccttagtgga tggtttggta gataacatta
      241 cccacaacaa aaaatatgtt gaggaagctg cccatgaagc cgtctgaagc gttgaacatt
      301 gcatgggatg ccattctcaa gaataagatc cgctccttct tgaccatgct aggaatcatc
      361 attggtgtct cggcggtcat catcatggtt gccatcagcg cagggacaga agccagtatt
      421 caagaacaga tcaccagcct tgggtcaaat ctagtattcg tacaaagctc catgatgcga
      481 ggcggacccg gtcaggcacc cgaaggtgga ctcgtctatg acgatgcggc tgccatcgaa
      541 accggtgtag acggtgtgac cgccgttgtg gtggaacaaa actccactga aactgtaaaa
      601 gctggcgata tcacgcttga agatgtggaa atcctcggca ccacgattgg ctttccatcg
      661 gtccgtgata tggaaattgc cgatgggcgt tacttcaacg aacaggaaaa tgaacgcaaa
      721 agcaaagtag ctgtgcttgg ttacaccgta gcacaagaat tatttggaga tacaaacccc
      781 gttgggcaat acatcaagat tggaaacacc aaagtcgccg taatcggagt gttcaaagaa
      841 aaagggttgg taggaaatac agactttgat tcacgcatct acctccccat caaactggtc
      901 ttccaaaaat ttacgccctc catgttcgcc cgatttatgg gtgatagcgt ccgcatgatt
      961 tatgttgaag tggacccaga tgccaatcaa gacgagctca ttacccagat cgaattgtta
     1021 ttagccaaac ggcacgacgt ttctctagac tcagcagact ttactgtcac aacacagcaa
     1081 gacatcatct caactcaaga gtcaacaacc tcagcctttc ggtcactttt ggcttgggtg
     1141 gcgggcgttt ctctgatcgt tggcggcatc ggcatcatga acattatgct ggtcagtgtc
     1201 accgaacgga cgcgtgagat cggcattcgg caatcaattg gcgccacccc agaagatatc
     1261 cgcttgcagt ttttgacgga ggccctattg ctcagcattg tcggcggtct gatcggagtc
     1321 atcgtaggcg ttggtggagc atacatcttc ggctctttaa gcgacatgcg aactgtgatc
     1381 tcactcccgt ccattctctt agcgtttggc tcttctgcca ttgtcggcgc atttttcgga
     1441 tattacccag ccaaccaagc ttcaaaactc gaccccattg aagcgttgcg atatgaataa
     1501 aggaaatttt atgaaaaaga tatttgtaat ttcattgatg ctatgttcat tagctctttc
     1561 tgcttgtagc agttccgcat caaccccaac aggtggaatg ccctcttcta acgacggagg
     1621 tgacatgagc accatcacca aacttgctat tggaacactc aaactagaag gaaccgagaa
     1681 tgccatcacg cctgagcaag ccagtgacct tttgcctctt tggcaagttt atctctcact
     1741 agtagacagc gataccgcgg ctcaagcaga aatcgacggc ttggttgacc agattagcac
     1801 aacactgacc gattcccaaa accaagcgat tgacgacatg caactttctc agtcagacat
     1861 gatgtctgtg atgcaagaaa tgggcatttc catgggaaac cgtccacaaa gcgctagtga
     1921 tagcgaacaa agcggaagct cgggaggctt tcccggtggt ggcgatgggt ttgctccacc
     1981 tgatggtggt ggtatgcccg gtggtggcat gcccagcgga gacatgcc
//
LOCUS       DAOQNJ010000513         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_47061_length_2028_cov_7.172326, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000513 DAOQNJ010000000
VERSION     DAOQNJ010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47061_length_2028_cov_7.172326"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLG52_12355"
     CDS             complement(<1..765)
                     /locus_tag="PLG52_12355"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HNB42287.1"
                     /translation="MVNVIGPDISFYQDDPQTPQGVNFIKMRESAYYVIVRAGQNLWG
                     DRDFKVNWRESKAAGLPRGSYWFYDSRIDPKQQAEKWISMFDGDFGELPLWGDFEDTY
                     NGPYKGWKNWYNFLERLKQLLPSDKEVGVYTGYYYWRENTVAVGIPNASLNYFKQYPL
                     WIANYGTSAPLVPEPWDTWTLWQFTDNGDGPAYGVESLNIDLNYFNGDLEALKTRFKL
                     GDLNFPPAPTPSNYYRVTVSSLKVRDAAGLNSTQIGS"
     gene            complement(827..1180)
                     /locus_tag="PLG52_12360"
     CDS             complement(827..1180)
                     /locus_tag="PLG52_12360"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNB42288.1"
                     /translation="MTKHNQRVGAWGEETAAVWLAGKGYEVSRRNVRTPYGEIDIIAQ
                     KNGVVYFIEVKTLTSSKEFFPEHQITAKKLEHMLNSAAHYAAELGIDRWQIDVIAIEG
                     KPGVEPRIQHFENIQ"
     gene            complement(1177..>2028)
                     /locus_tag="PLG52_12365"
     CDS             complement(1177..>2028)
                     /locus_tag="PLG52_12365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB42289.1"
                     /translation="LYAQAYLPAMPEEVSIGMLQAYMPPFCKAVDLVITPSAGMARVL
                     RELKVESDVEIVPNGVDLTNFQNAHPLEREPFGYKPDDILIVYAGRIALEKNLPFLIE
                     SFNGIANAIPNVHLILIGGGVQQYEEEIRDVVKAQMNSDRIHMTGRVPYDQLPAYLAM
                     CDIFATASITEVHPLSVIEAMGAGLPTMGIESVGVGDTVEDGVTGFLATNDMPSFTAK
                     LTRLCLDPELRQQMRISARKASTAYAIERTTGLMLEKYKHLTSEDFSHKGSFNTRLRE
                     ILERFLT"
BASE COUNT          528 a          490 c          459 g          551 t
ORIGIN      
        1 gctcccgatc tgagtggaat tcaaacccgc cgcatcgcga actttgagtg aagaaacggt
       61 cacgcgataa taattgctgg gggtaggggc tggagggaag ttaaggtctc ccagtttgaa
      121 gcgggttttg agagcttcca gatcaccatt gaaatagttc aaatcaatat tgaggctttc
      181 aaccccatac gcaggtccat ctccattatc cgtgaattgc caaagcgtcc atgtatccca
      241 aggctcagga acgagcggag cgcttgtccc ataattggca atccataatg gatattgttt
      301 gaaataattc agcgaagcat ttggaattcc gaccgcaact gtattttcgc gccagtagta
      361 atacccagtg taaacaccaa cttctttatc agaagggagc aactgtttca acctctctaa
      421 aaagttgtac cagtttttcc aacctttata aggaccattg tacgtatctt caaaatcgcc
      481 ccaaagtggc aattcgccaa aatcgccgtc aaacatcgag atccattttt cagcttgctg
      541 tttcgggtca atgcggctgt cgtaaaacca ataagaaccg cgtggcaaac cggctgcttt
      601 tgactcacgc caattgactt tgaaatccct atcgccccat aaattttgcc ctgcacgcac
      661 gatcacataa taagcgcttt cacgcatctt aataaaattg acgccttgcg gagtctgtgg
      721 atcgtcctga taaaaactaa tatcgggtcc gatgacattt accatgagag tctcctcgtt
      781 gttcgttagc cgtatgcttt caggttttgt aaattatatc tgatacttat tgaatatttt
      841 caaagtgttg aatgcgcggt tctacgccgg gttttccctc gattgcaatc acatcaattt
      901 gccaacggtc aattccaagt tcggcggcat aatgagccgc tgagttaagc atatgttcca
      961 gcttttttgc agtgatttga tgttcaggga aaaattcttt cgaagaagtt agcgtcttga
     1021 cctctataaa atagacgacg ccatttttct gggcgatgat atcaatctcc ccatacgggg
     1081 tgcggacatt tcgacggctc acttcgtagc ccttgcctgc cagccagacc gcggcagtct
     1141 cttcgcccca agcgcccact cgctgattat gcttggtcat gtcaaaaagc gttccaaaat
     1201 ctctcgtaga cgagtgttaa acgagccctt atgtgaaaaa tcttcactgg ttaaatgttt
     1261 atatttttcg agcatcaagc cagtggtgcg ttcaatggca taggcagtag aagccttacg
     1321 ggcagagatg cgcatttgct gacgcagttc cgggtccaga cagaggcgtg taagttttgc
     1381 cgtgaacgat ggcatatcat tcgttgccaa aaagcccgtc actccatctt ccaccgtatc
     1441 cccaacaccg acagattcaa tgcccatcgt tgggagcccc gcccccatgg cttcaattac
     1501 agaaagcgga tgaacttctg taatagaggc tgttgcaaaa atatcacaca tagcgaggta
     1561 ggcagggagt tgatcgtacg gcactctgcc cgtcatgtga atacggtctg aattcatctg
     1621 ggcttttact acatctcgaa tctcttcttc atattgttgc acgccgccac caataagaat
     1681 taagtggacg tttggaattg cattggcgat accgttaaac gactcgatca ggaatggtag
     1741 attcttttcc aatgcaatac gcccagcgta gacgatcaga atatcatcgg gtttgtagcc
     1801 aaatggctcg cgttcaaggg ggtgcgcatt ttgaaagttg gtaaggtcaa caccattggg
     1861 aacgatctcc acgtcactct ctactttgag ttcacgtagc acccttgcca tgcctgccga
     1921 aggggtgatg acaaggtcaa cagctttgca aaatggcggc atatacgctt gcaacattcc
     1981 aatgctaact tcttcaggca tggctgggag gtaggcttgt gcatagag
//
LOCUS       DAOQNJ010000514         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_47275_length_2023_cov_10.141260, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000514 DAOQNJ010000000
VERSION     DAOQNJ010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47275_length_2023_cov_10.141260"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PLG52_12370"
     CDS             complement(<1..1007)
                     /locus_tag="PLG52_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNB42290.1"
                     /translation="MEFKINNHTIGLNHPTYFIADIAANHDGDLDRAKLLIRLAKEAG
                     ADAAKFQHFQAPKIVSEYGFTHMNSKVSHQANWKRSVTEVYAAASVSQSWTPILKEEC
                     DKVGIDFFTSPYDYDSIEHVNPYIPAFKAGSGEIDWIEALVHMASKGKPMIIACGAAD
                     IADVQRAVQAILKVNKQLCLMQCNTNYTANPNNYDHLHLNTLKTFAAMYPDVILGLSD
                     HTHSVAPVLGAITLGARMIERHFTDSNDREGPDHKFAMNPANWAKMVEETRLLERSLG
                     SSDKFIAANEQETKVVQRRCLRAARDIKAGETFTRDMIDVLRPATPGAVKPHEIENVI
                     GT"
     gene            complement(1040..1474)
                     /locus_tag="PLG52_12375"
     CDS             complement(1040..1474)
                     /locus_tag="PLG52_12375"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB42291.1"
                     /translation="MIDKYHLLDFEREAVVALMENADGEVIFALIPRYPSNTVEWELP
                     AGGMEVGEEILEAAKRELLEETGYSSTDHKLIYTYYPMNGNANQVFHIVFCKAGERVQ
                     DFDLNEVADVRWFKREEIKQLLKDQKIKDGFSLTALLLWLQN"
     gene            complement(1826..>2023)
                     /locus_tag="PLG52_12380"
     CDS             complement(1826..>2023)
                     /locus_tag="PLG52_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42292.1"
                     /translation="ESDITPKSVNFSGLIAKRSNNLWLSTRKLSADLGEDLPNFSTGL
                     DEFYTQYEQGYPQKIRGYQQP"
BASE COUNT          515 a          449 c          501 g          558 t
ORIGIN      
        1 gtgccgatga cattctcgat ctcatgcggt ttgaccgcgc ccggtgtggc ggggcgcaga
       61 acatcgatca tatcgcgggt aaaggtctca ccggctttga tatcgcgggc tgctcgcaga
      121 cagcgacgtt gaacgacttt agtctcttgc tcattggcag caatgaattt atcggagcta
      181 cccagcgaac gttcgagcaa acgagtctct tcgaccatct tcgcccaatt ggcaggattc
      241 atcgcaaact tgtgatcagg tccttcgcgg tcgttgctgt cagtgaagtg acgttcaatc
      301 atgcgtgcgc cgagagtgat cgcgccgaga accggcgcaa cagaatgcgt atggtcagac
      361 aaaccaagaa tcacatcggg gtacatggcg gcgaaggttt tgagcgtgtt caaatgcagg
      421 tgatcgtagt tattggggtt ggcggtgtag tttgtgttgc attgcatgag acacaactgt
      481 ttgttgactt tgagaatggc ttggacggca cgctgtacgt cagcgatatc agcggcgccg
      541 caagcaatga tcattggctt acctttggac gccatgtgga cgagcgcttc gatccagtca
      601 atttcgccag agcccgcttt aaaagctgga atgtatggat tgacatgctc aatcgagtca
      661 taatcgtaag gtgatgtgaa gaagtcgata ccaactttat cgcattcttc tttgagaatg
      721 ggcgtccaac tttgagaaac agaagccgcc gcatagactt cggtaacgga acgcttccaa
      781 tttgcctggt ggctgacctt ggaattcata tgagtgaaac cgtactctga aacgatcttc
      841 ggcgcttgaa aatgctggaa cttggcagca tcggctccgg cttctttggc gaggcggatg
      901 agtagcttgg cacgatccaa atcaccgtcg tgatttgctg cgatatcagc gatgaagtag
      961 gtcgggtgat tcaatccgat agtatggtta ttgattttga attccatgtc gtctctcctt
     1021 tatttcgatg aaagattatt tagttttgca gccataaaag caatgcagtt agtgaaaagc
     1081 catccttgat cttttggtct ttcaacaact gtttgatctc ttcccgctta aaccagcgaa
     1141 catcagccac ttcattcaag tcgaaatctt gcacgcgttc accggctttg caaaatacaa
     1201 tatgaaagac ctgattggca ttcccgttca tggggtaata ggtatagatc aatttgtgat
     1261 cggttgagct ataccctgtt tcttccaata actcgcgttt ggcggcttcg agaatctctt
     1321 caccaacttc catgccgcct gcggggagtt cccattcaac cgtattactt gggtagcgag
     1381 ggatcagggc aaaaattact tccccgtccg cattttccat aagggcaacc acagcttcac
     1441 gttcgaaatc caataaatgg tatttatcga tcacaaaacc attcggaaat ctcacagtgt
     1501 ccttgtataa attaacccac ctactttggt acacgacctc tcttgccaat ctttctggct
     1561 tcatggtgaa agaaaatctc ctcgtaactg ttttatcaac aagggctgtg ggtaaaacag
     1621 tggataaaca cgtcacagag ctgggttatt gtgtgtaaac gtactccagc ctgtggataa
     1681 gtgtgtggat tctgtggata actcggttca aaacctcggg attttggcaa gatctgcctg
     1741 attcctgtgg aaaaacccca atttaagtgg ggaaatctgt ggataagtgg cagaaaaaga
     1801 tgccaaaaat cggctcaaac tgtggctaag gttgttgata accacgaatt ttctgtggat
     1861 aaccttgttc gtattgtgtg taaaactcgt ccaaacctgt ggaaaagttg gggagatcct
     1921 cacccaaatc tgcggataac ttgcgggtag aaagccataa attgttggaa cgtttggcaa
     1981 ttaatccgga aaagttgaca gattttgggg taatgtcact ttc
//
LOCUS       DAOQNJ010000515         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_47951_length_2007_cov_5.677254, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000515 DAOQNJ010000000
VERSION     DAOQNJ010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47951_length_2007_cov_5.677254"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLG52_12385"
     CDS             complement(<1..649)
                     /locus_tag="PLG52_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB42293.1"
                     /translation="MALDLSVIRAQFPSLDRPAIFFDNPGGTQIAKQSLDRITRYLIE
                     NNANHEGVFATSKASDAVLDEAHQAMADFYNAPSAKEIVFGNNMTTLTLHISRSISRD
                     WKEGDEIVLTRLDHDANVTPWVLAAQDKGVKINWVDFDVEDGTLKLDDMQKALERKPK
                     LVAVGYASNSLGTVNPIQKITQMAHDAGALVYVDAVQYAVHGPIDVQKIGCDFLVS"
     gene            746..1618
                     /locus_tag="PLG52_12390"
     CDS             746..1618
                     /locus_tag="PLG52_12390"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB42294.1"
                     /translation="MTQLSTKLKVAIHEAFLPVLRFILPVVRWLDKKTYYHEALRLNR
                     ELFSHVSNIPVEEIDFVKNKIREIANAGKYDATYRETWVSHGPRLALSKRWIQEALQD
                     RSGNLTGLDLGTRSIATELWSLWFPQVQWRNTDFDLRASWDVPTSSVDVVVCTELVEH
                     LPDVLDGKTFNETFVASGLKGLLGEAWKALKPGGFLFITTPNASSVLHVKAVLEKRPP
                     WFFDRHIREYTLDELLPFIQQAEFHVQRAEAVHCMTVFGITDFVAQFEFLVANGYPTD
                     GRGDDLFILARKPT"
BASE COUNT          503 a          461 c          522 g          521 t
ORIGIN      
        1 aagagaccag aaaatcacag ccgatctttt gtacatcgat ggggccatgc acggcgtact
       61 gaacagcatc cacgtacacc aaggctccgg catcatgcgc catctgtgtg atcttttgga
      121 tggggttgac agttccgagc gagttggagg catagccaac agcgaccagt ttcggcttgc
      181 gttcaagcgc tttctgcatg tcgtccagct tgagcgtgcc atcctccacg tcgaaatcga
      241 cccaattgat ctttacgcct ttatcctgcg cggccagcac ccagggtgtg acgttggcgt
      301 cgtggtcaag gcgggtgagc acaatctcgt cgccttcctt ccaatcgcgc gaaatagagc
      361 ggctaatatg cagggtgagg gtggtcatgt tgttgccgaa gacgatttct ttggcagagg
      421 gtgcattgta aaaatctgcc atggcttggt gcgcctcgtc gagtacggca tcagaggctt
      481 tgctggtggc aaagacacct tcatgattgg cattgttttc aataagatat ctggtgatgc
      541 ggtctagaga ttgttttgcg atctgcgttc cgccggggtt gtcgaagaag atcgccgggc
      601 ggtcgagcga agggaattgg gcacggatga cggataggtc gagagccata tggtaaactc
      661 cattctggaa atgaacaatg agcggtatta tagtctattg aaggacgcag gtttccaaat
      721 aaattcccaa aacggccata tctaaatgac tcaactttct acaaaactta aagtcgccat
      781 tcatgaggct tttcttcctg tcttacgctt cattctgcct gttgtgcgtt ggttggataa
      841 gaagacctac tatcatgagg ccctgcgatt gaaccgggaa ctgttttcgc atgtgagtaa
      901 tattcctgtg gaggagatcg atttcgtaaa gaacaagatc cgcgaaattg caaacgccgg
      961 gaaatatgat gccacctaca gggagacatg ggtatcgcac gggccgcggc ttgcgctttc
     1021 caaacgatgg atacaagagg cgttgcagga tagatcgggc aatctgaccg gccttgatct
     1081 tgggacaagg agcattgcca ccgagctttg gagtctgtgg ttcccccagg ttcaatggag
     1141 aaataccgac tttgaccttc gggcctcttg ggatgttccc acatcatctg tggatgtggt
     1201 tgtttgcacg gaattggtcg aacacctgcc cgatgtgttg gatggtaaga ccttcaatga
     1261 aacctttgtc gccagcgggt tgaaaggcct gttgggggaa gcctggaaag ccctcaagcc
     1321 cggcggattt ctcttcatta ccacgccgaa tgcctcctcc gttctgcacg tcaaagccgt
     1381 gcttgaaaaa cgcccgccct ggttttttga ccgtcatatc cgtgaataca cgttggatga
     1441 attgctgccg ttcatacaac aggcggaatt tcatgtccag cgtgccgagg cggtacattg
     1501 catgaccgtg tttggcatta ccgatttcgt tgctcagttt gaatttctgg ttgcaaacgg
     1561 gtatcccacc gatgggcgtg gtgatgattt gttcatcctt gcccggaaac caacttaatt
     1621 taacccaagt tgcggcctac agggagagcg cccaaaattt aacgcaaagg cgcaaggacg
     1681 caaagaaaaa gctttcgatc ttggcgcctt tgtgttaata aatctcgacc aagcccgcca
     1741 aaatgtacaa ggtagcaacc tgagttacag agtatcgcat aatttggtaa acaaaaattc
     1801 gattttcctt cgtgattttc aaaggaaagt ttacttattt atgagacgag cgataaatta
     1861 tagaaaaaac acatgaggga aaacctgtga caaaccacac tccaatttga tgatttgatt
     1921 gatagctctt ccaacgaaat tcagatgcac cggttgtata ctgagtttga cgtttcggaa
     1981 aggagacatc atgttcaata aaattct
//
LOCUS       DAOQNJ010000516         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_48039_length_2005_cov_8.260513, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000516 DAOQNJ010000000
VERSION     DAOQNJ010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48039_length_2005_cov_8.260513"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /gene="tpx"
                     /locus_tag="PLG52_12395"
     CDS             <1..528
                     /gene="tpx"
                     /locus_tag="PLG52_12395"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNB42295.1"
                     /translation="EMTTIRNDVLKLGDKFATLFGDDVKVGQAAPEFTSVVAGWAAVN
                     PIQESKGKVIILSAVPSLDTDVCDRETRRFNEEAAKLGDDIVIYTISTDFPMAQKRWC
                     GAAGVDKVKVVSDVLEAEFGIKYGLLIKERRFLRRAVFIVGHDGKFTYVNYLPTLGNE
                     PDYDEVISEAKKALG"
     gene            634..1980
                     /locus_tag="PLG52_12400"
     CDS             634..1980
                     /locus_tag="PLG52_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HNB42296.1"
                     /translation="MSDKSVIIIGAGMAGLSAAHEFNKAGWAVTVLEARSRVGGRVHT
                     VREFSHGLVAEGGAEYINESQHRMIALAKEFDLYLGAVGSWQNQSGDWAALQGKAGYL
                     HDTVLWGADLDAENTKVWVALAELGKQVTDLSNPAASKNAKALDSQNAESWLQNLNVH
                     PLAKVLFANHIRSEYTCEPKDFSLLDLARNAAMYYSDPNASDPAYRIIGGNDQLPQAM
                     AKRLPNVRMNAVVTSIKLLDEKVEVVYKLADTFHTLQASYAILATPLTTARLIDFEGT
                     LPPAHHAMVHELSYGSVTKVMIEYKKRFWHDRGWNGRLNTDLPIVFTWDATSHLEGEH
                     GILTAYTGGEPGAALTRLSDTDRIKTAVSAIESIFPGSSNLIENTQTIAWRNEAFTLG
                     SYMAYAPSQVTSHWQALFSPAGRLYFAGEHATVYQGFMEGAVESGQRAAKSIIEKG"
BASE COUNT          547 a          467 c          501 g          490 t
ORIGIN      
        1 gaaatgacta ctattagaaa tgatgttctt aagctgggcg ataaattcgc cacccttttt
       61 ggggatgatg tgaaagttgg gcaagcggca ccagaattta ccagtgttgt agcaggttgg
      121 gcggctgtaa acccaattca ggaatcgaaa ggaaaggtga ttatcctttc ggctgttccc
      181 tcgctggata cagatgtctg tgaccgtgaa acccgtcgct ttaatgaaga agccgctaaa
      241 ctgggtgacg atattgtgat ctacaccatc agcacagact tccctatggc gcaaaagcgc
      301 tggtgtggcg ccgccggtgt ggacaaggtc aaggttgtgt ctgatgtact tgaagctgag
      361 tttggcatca agtatgggct gttgatcaag gaacgccgct ttttacgtcg cgccgttttc
      421 attgtgggac acgatggcaa attcacgtat gtcaactacc ttcccacgct cgggaatgag
      481 ccagattacg atgaagtaat ctcagaagca aagaaagctc tcggctaaat aaaaactaaa
      541 tgggcagtac gcagcccaag tcttgactat gaggcgaagc cgtcctgcat gttgggacgg
      601 ctttttatat cctaatgata aaatccacaa actatgagcg ataaatctgt catcatcatt
      661 ggcgcaggca tggcgggact ttccgccgcg cacgaattca acaaagcagg ttgggctgtc
      721 actgtattgg aagcgcgttc acgcgtaggc ggacgtgttc acactgtgcg cgagttttcg
      781 catgggcttg tagcagaggg cggcgcagaa tacatcaacg aaagtcagca tcgcatgatc
      841 gcgcttgcaa aagagttcga cctttacttg ggggctgtag gctcctggca aaaccaaagt
      901 ggagattggg cagccttgca aggcaaagct gggtatttac acgatacggt tttgtggggt
      961 gcagaccttg atgcagagaa tacaaaagtt tgggtggcgc tggcagaact tggaaaacaa
     1021 gtaacggatc tttcaaaccc tgcggcatcc aaaaatgcaa aagctttaga tagtcaaaat
     1081 gcagagagtt ggcttcaaaa cctgaacgtt catcctttgg caaaggtgct atttgcgaat
     1141 catattcggt cggaatatac atgtgaacca aaagattttt ctcttttaga tttggcacgt
     1201 aatgccgcca tgtattacag tgacccaaat gcctctgacc cagcatatcg catcattggt
     1261 ggtaatgacc aacttccgca agcgatggca aaacgattgc caaatgtgcg gatgaatgct
     1321 gttgtaacct ccatcaagct gttggatgaa aaagttgaag ttgtttacaa actagctgac
     1381 actttccata cgttgcaagc ttcatacgct attcttgcaa cgcctcttac gactgcacgc
     1441 ctgatcgact tcgaagggac tttacctcct gctcaccacg ccatggtcca cgagctttcc
     1501 tatggctccg ttaccaaagt gatgatcgaa tataaaaagc gtttttggca tgaccgcggc
     1561 tggaatggac gcctcaatac agacctgccg attgtcttca catgggatgc taccagccac
     1621 cttgaaggcg aacatggaat cctcacggct tacacaggcg gcgaacccgg tgccgcccta
     1681 actcgccttt ccgatacaga ccgcatcaaa acggcagtct ctgcaattga atctattttc
     1741 cctggttctt caaacttaat cgaaaatacc caaaccattg cctggagaaa cgaagccttt
     1801 acattggggt cttacatggc atatgcaccc agccaagtca cctctcactg gcaagcgctc
     1861 ttctcccctg ctgggagact atattttgcc ggcgaacatg ccaccgttta tcaagggttc
     1921 atggaagggg cagtggaaag cggtcaacgc gcggcaaaaa gcattataga aaaaggataa
     1981 agaatgagtg aagcaagaag gaatt
//
LOCUS       DAOQNJ010000517         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_48083_length_2004_cov_11.097486, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000517 DAOQNJ010000000
VERSION     DAOQNJ010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48083_length_2004_cov_11.097486"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PLG52_12405"
     CDS             complement(<1..892)
                     /locus_tag="PLG52_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42297.1"
                     /translation="MNQNVPSYTGDIAAQQIDPSQEYSQENQEISFSAEHHAIWTDLF
                     AGIHQPYLMEHICNEWIKGMELLKLDPKRIPTVKHLIENITPRSGWHIERTVVRYTLA
                     DDWYKKFAQRVFLITDYLRTRDQMEFTPEPDMFHDIFGHLPFLTQEFYARIEDKFAPA
                     YMKATQEEREVIKRLAWYSTEFGLVVQNNRFTVFGAGIISGRKELTRTIMEFYRLGRD
                     NVIDYSKDVYEQLNNHFLANKSNLYKIIDGVNALHQKGQMSSADEGWNVVLGLYKSLG
                     IKPEGIFGGEVILVPFNVETI"
     gene            1023..1772
                     /locus_tag="PLG52_12410"
     CDS             1023..1772
                     /locus_tag="PLG52_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB42298.1"
                     /translation="MHDLTGKTILITGAARRLGRLFALACGRAGADLILHHGHSPAEA
                     ESLKDELASLGRRAWVLAADFSHPESASQLIERANELSPLYGLVNSASIFEPLSMHET
                     SLDDWNNHLNVNLTAPFLLSQAFAKSNAHAAVHGGEVKGRIINILDWRALHPGADHFP
                     YTVTKSALAAMTKSLAIALAPNITVNGLALGAILPPADNPSASEKIIEAVPAKRWSEA
                     NEVEEALLFLLSGPAYITGEILHVDGGRHLV"
BASE COUNT          488 a          544 c          482 g          490 t
ORIGIN      
        1 aaatggtttc gacattgaac ggcacaagga tcacctcacc gccgaagatg ccttcaggct
       61 tgatacccag gctcttgtac aagcccaaaa cgacgttcca accttcatcc gcagaggaca
      121 tctgtccctt ttggtgaagc gcgttcacgc cgtcaatgat cttgtacaga ttgctcttat
      181 tcgcgaggaa gtggttattc aattgctcgt acacatcctt gctgtagtcg atcacattgt
      241 cacgcccgag gcggtagaac tccatgatgg tacgggtcaa ttccttgcgc cctgaaatga
      301 tgcccgcgcc gaaaaccgta aagcggttat tttgcaccac caagccaaac tcagtgctgt
      361 accaggccag acgtttgatg acctcgcgct cttcctgagt tgccttcata taggcaggtg
      421 caaatttatc ttcaatgcga gcataaaatt cttgtgtgag aaacggtaaa tgcccgaaga
      481 tgtcatggaa catatccggc tcaggtgtga attccatctg gtcgcgggtg cgcaaataat
      541 cggtaattaa aaatacacgc tgcgcgaatt ttttgtacca atcatcggct aacgtgtatc
      601 tcacaacagt tcgttcaatg tgccaacctg aacgcggcgt gatgttctca ataagatgct
      661 tcacagttgg gatacgcttc gggtcgagct tcaacaactc catccccttt atccactcat
      721 tacaaatatg ttccatcaaa tacggctggt ggatgcccgc gaacaggtct gtccaaatgg
      781 catggtgctc ggcggaaaac gagatctcct gattctcctg cgaatattcc tgcgaggggt
      841 caatctgttg cgctgcgata tctccggtat agcttggtac gttttggttc atgttcacct
      901 ttacaccttt ctgagttgga cttgggggat acagaactta tcgaaaagat agtatcacct
      961 ctaaaaaccc gagtcaagaa gcggagaaga attgtcataa ttagcggtgt tagaatccac
     1021 ccatgcatga tctaaccggc aagaccatcc tcatcaccgg agccgcccgc cgcctggggc
     1081 gtttgttcgc tcttgcctgt ggacgcgctg gcgctgacct catcctccat catggacatt
     1141 cgcccgccga agccgaatcc ctcaaagatg aactcgcctc tctcggacgc cgcgcctggg
     1201 tcctcgctgc ggacttttct caccctgaaa gcgcatcgca attaatcgaa cgcgctaatg
     1261 aactcagtcc gctatatgga ttggtcaaca gcgcatccat ctttgagccg ctttccatgc
     1321 acgaaacatc actcgacgat tggaacaacc atctcaacgt caatctcact gcaccgttct
     1381 tgttgagcca ggcgtttgca aaatctaacg ctcatgccgc cgtccacggc ggcgaggtaa
     1441 agggtcgcat catcaacatc ctcgattggc gtgccctgca ccctggcgcg gaccacttcc
     1501 cctacaccgt caccaaatct gcgctagcag cgatgaccaa atcactagcc atcgcgcttg
     1561 caccgaacat cactgtaaat ggattagcgt tgggcgccat cctgccaccc gcagataacc
     1621 cgtctgcgag tgaaaagatc atcgaggcgg taccagccaa aagatggagt gaggcgaacg
     1681 aagtcgaaga agcgctgctg tttttattgt caggtcccgc ttatatcacc ggcgagattt
     1741 tgcatgtgga tggcggcagg catttggtgt aattaccatt ccgtcattgc gaggagcgct
     1801 cttgttcttc gcgacgaagc aatctcgcat acagtgtcat gagattgctt cgggcgaaag
     1861 tgcatcgccc tcgcaatgac gatgaattag attaggagcc ctcatggaca aagtatttat
     1921 caaagacctg ctcgtgcgtg gcattatcgg tatccgcgag tgggagcgtg agaaagagca
     1981 ggatattctc atcaacgtga cggt
//
LOCUS       DAOQNJ010000518         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_48242_length_2001_cov_9.819630, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000518 DAOQNJ010000000
VERSION     DAOQNJ010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48242_length_2001_cov_9.819630"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLG52_12415"
     CDS             complement(<1..620)
                     /locus_tag="PLG52_12415"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB42299.1"
                     /translation="MTDRIETFHYDEQNPDRLDKYLVSCLPEFSRARIQGLILDGFVS
                     INGVPAKKAGQVIEDGDDIEVRVPPPVPSGLVAEDIPLDIIFENDDLIVVNKPAGMVV
                     HPAAGHYSGTLVNAVLGYDPDLEGIGGEERPGLVHRLDKETSGLIILAKNERAHNWLQ
                     DQFRLRKVEKTYLALVDGKPPTPAGRVEAPIGRDPSHRKKMAIVSPG"
     gene            complement(617..1162)
                     /gene="lspA"
                     /locus_tag="PLG52_12420"
     CDS             complement(617..1162)
                     /gene="lspA"
                     /locus_tag="PLG52_12420"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNB42300.1"
                     /translation="MTEEISVQEKTQPRSRRFFIFFAVAIVIIALDQWTKWLVIQSVP
                     LNTSWLPEALDWLSPYARLLHIQNRGVSFGMFQGGNTVFIILTTVIVAAIVFYVSRMD
                     HPSDWMWVAAGLYLGGAVGNLIDRLTIGAVTDFISVGNFYIFNIADASINISVVLLLL
                     LSWLHERRSSTLETEPKDVSA"
     gene            complement(1167..1706)
                     /gene="lspA"
                     /locus_tag="PLG52_12425"
     CDS             complement(1167..1706)
                     /gene="lspA"
                     /locus_tag="PLG52_12425"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNB42301.1"
                     /translation="MNSKLRDYLMLFGVAGVSVALDQWTKWLVRTNIEYSGQWLPDWL
                     NWLSPYARIVHWYNSGAAFGMFQNGNLIFTILAFVVIAAILYYFPSVDSDDWTLKLAM
                     GLQLGGAVGNLIDRLIMQKVTDFISIWTFPVFNIADASISVGVAVLLLGVWLKEREEK
                     LKAAQVKTEAVPETMKEGS"
     gene            complement(1733..>2001)
                     /locus_tag="PLG52_12430"
     CDS             complement(1733..>2001)
                     /locus_tag="PLG52_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HNB42302.1"
                     /translation="YADGYFRRMTNQGRVLINGKSYSQVGRICMDQFMVDAGSDEIVI
                     GDEVILLGSSIAPEELADWAGTNEYEVMTNISARVPRVFVGGVQ"
BASE COUNT          573 a          559 c          387 g          482 t
ORIGIN      
        1 cccggtgaga caatcgccat ctttttgcgg tgacttgggt cacgcccaat cggcgcttcc
       61 acacgtccag ccggtgtagg tggtttgcca tctacaagcg caagataggt tttttccact
      121 tttcgcaagc ggaactggtc ttgcagccag ttgtgagcgc gttcattctt ggcaaggatg
      181 atcaaacctg aagtctcttt atccaaacga tgtaccaaac cggggcgttc ttcgccacca
      241 ataccttcaa ggtctgggtc ataccctaat actgcattga ccaaggttcc agagtaatgc
      301 cctgccgccg ggtggacgac cataccggca ggtttgttca ccacaatcag gtcatcattt
      361 tcaaaaataa tatcaagcgg aatatcttct gccaccaatc cacttggcac tggcggcgga
      421 actcgcactt cgatatcgtc gccatcttca attacttgcc ctgctttttt agcaggcaca
      481 ccattgatcg atacaaatcc atccaaaatc aagccctgaa tacgggcacg tgaaaactca
      541 ggcagacagg aaacaagata tttatccagc cggtctggat tctgttcgtc ataatggaat
      601 gtttcaattc ggtcggtcat gcgctcacgt ccttgggctc agtttcaagc gttgacgatc
      661 ttcgttcatg caaccaagat aaaagcaata acaaaacgac actgatgttg atactagcat
      721 ctgcaatatt gaagatgtaa aagttaccca cggaaatgaa atccgttacg gctccaatcg
      781 tgaggcggtc gatcaagttt ccaacggcac cgcccaaata caagccagcc gccacccaca
      841 tccaatcact ggggtgatcc atacgagaca cataaaagac aatcgccgcc acgatcacag
      901 tcgttaagat gataaacacc gtattcccgc cctggaacat accaaacgaa acaccgcgat
      961 tctgaatatg gagcaaacgg gcatatggcg aaagccaatc caacgcttca ggcaaccaag
     1021 atgtgttgag agggacagat tgaatcacca gccattttgt ccactggtcg agggcaatga
     1081 tcacgattgc cacggcaaaa aagataaaaa aacgcctgct acgaggctgc gttttttctt
     1141 gtacactgat ttcttcagtc atcaaactac gaaccttctt tcattgtttc tggcacggct
     1201 tccgttttta cctgagccgc ctttagtttc tcttctcgtt ccttaagcca tacccccaat
     1261 aataaaaccg ctacccccac cgaaatggat gcatcagcaa tattaaaaac tgggaaggtc
     1321 cagatcgaaa taaaatctgt caccttttgc ataatcaaac ggtcgatcaa atttccaacc
     1381 gcaccgccca attgcaaccc catcgccaat ttcaacgtcc aatcatctga gtcaacactg
     1441 ggaaagtaat aaagaatggc agcaatcaca acaaatgcca aaatagtgaa aatcaagttt
     1501 ccattttgaa acatgccaaa cgcagcgccg ctgttatacc aatgcacaat acgtgcataa
     1561 gggcttaacc aatttaacca atcaggtaac cactgcccac tatattcaat atttgtacgt
     1621 accagccact ttgtccactg atcgagtgca accgaaacgc cagccacgcc gaacagcatt
     1681 aaataatctc ttaacttgga attcaactct cacctcaaaa cgaaaagtgc gcctattgta
     1741 ccccacccac aaataccctc ggcacgcgtg cgctgatgtt ggtcatcacc tcatattcgt
     1801 tggtgcctgc ccaatctgcc aactcttctg gcgcaatgct gctgcccaac aatatcacct
     1861 catcccctat cactatttca tcgctcccag catccaccat aaactgatcc atgcaaatac
     1921 gacctacttg cgaatacgac ttgccgttga tgaggactcg tccttgattc gtcatgcggc
     1981 ggaagtagcc atcggcatat c
//
LOCUS       DAOQNJ010000519         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_48245_length_2001_cov_8.317061, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000519 DAOQNJ010000000
VERSION     DAOQNJ010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48245_length_2001_cov_8.317061"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /gene="ccsA"
                     /locus_tag="PLG52_12435"
     CDS             complement(<1..1330)
                     /gene="ccsA"
                     /locus_tag="PLG52_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNB42303.1"
                     /translation="MFSEFGFGVLLISFIVTLYSGIAAVYGVRSKSASVVESARRAQL
                     LTFPLLSIAAGSLIYLLITNHFEVSFVYEVTSKSMPTYLKVTAWWGGQAGSLLFWAWL
                     LAAFTSAVTLRKWNRDIEFLPWVIVVSSITLAFFLGMVVFYENPFSRFWQDAAGQIVM
                     NAAGDPIKSIFAPAGAVSTFLPSDGRGLNPLLRHPGMVIHPPMLYLGFVSFVIPYAFA
                     MAALITGRTDDRWIRITRRWSLWAWLFLSLGLVLGGRWAYDVLGWGGYWGWDPVEIAA
                     FMPWLTGTAFLHSVMIQEKRGLLKHWNMILIILTYGLVIFGTFLTRSGVLSSVHAFAN
                     SPIGPFFMGFVSLTLIASIALLIKRWPELKSETEMKSMLSREALFLLNNLLFMGILVV
                     CFWGVISPLASELFTGTKITVGPPYYERATGPLFAGLLFLMAVAPLSAWGH"
     gene            complement(1448..1930)
                     /locus_tag="PLG52_12440"
     CDS             complement(1448..1930)
                     /locus_tag="PLG52_12440"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNB42304.1"
                     /translation="MQRNKFIIGGVLILAAVVYLIASSTQASAEYFMTVDELNAEGSS
                     AINKSVRLSGAVIGDTIQYDASTLTLTFEVAHVPGDNAEIEAQGGLAAVLHEAVMDTS
                     RARIKVQYEGVMPDLLRNEAQAIMTGKLGDDGVFHAEELLLKCPTKYEEAVPEQASAN
                     "
BASE COUNT          577 a          509 c          523 g          392 t
ORIGIN      
        1 aatgtcccca agcagagagc ggcgcaactg ccatcaagaa gagcaaccca gcaaatagcg
       61 gacctgtagc acgttcgtag tagggaggtc caaccgtaat tttggtgccg gtgaaaagct
      121 cagacgccaa tggcgagatc acaccccaga aacaaactac taagatgccc atgaaaagca
      181 agttattgag aaggaagagc gcctcacgtg aaagcatgga cttcatctca gtttcgcttt
      241 tcaattcggg ccagcgtttg atcaataaag ctatcgaggc aatcagggtc aagctcacaa
      301 agcccatgaa gaaaggacca attgggctgt tcgcaaaggc gtggacggaa gaaagcacac
      361 ccgagcgggt gaggaaggta ccgaagatca cgagaccata ggtcaagatg atgaggatca
      421 tgttccagtg tttgagcaaa ccgcgctttt cctggatcat gaccgaatgt aagaaagcgg
      481 ttccggttaa ccacggcatg aaggcggcaa tttcaaccgg gtcccagccc caatagccac
      541 cccaaccgag cacatcgtat gcccaacgtc cgccgagcac gagaccaagc gagaggaaga
      601 gccaagccca caaagaccaa cggcgtgtaa tgcgaatcca gcggtcatcg gtacgaccag
      661 taatgagcgc agccatcgca aaggcgtagg gaatcacgaa agagacaaag ccaagataaa
      721 gcatgggcgg atgaatgacc atgcctgggt ggcgaaggag cgggttcaac ccacgtccat
      781 cactgggaag aaatgtggaa acggcacccg ctggagcgaa gatacttttg atcgggtctc
      841 cagccgcatt catcacgatc tgaccggcag catcttgcca gaagcgtgag aaggggtttt
      901 cgtaaaagac caccatgcca aggaagaacg ccaaagtaat ggaagagaca acgatcaccc
      961 aaggcaggaa ctcgatatca cggttccatt tgcgcaatgt aacggcagat gtaaaagccg
     1021 ccagcagcca agcccagaac aaaagcgagc cagcttgtcc gccccaccag gcagtcactt
     1081 taagataggt aggcatgctc ttgctggtca cttcatagac aaacgaaact tcgaagtgat
     1141 tcgtgattag caagtagatc aaggaacccg ctgcgatgga aagcagaggg aaggtgagta
     1201 gttgagcgcg ccgggcactt tccactacag aagccgactt actccgaaca ccatagacgg
     1261 cggcgatacc gctgtagagt gtaacgatga aactaattag taaaacgcca aagccaaatt
     1321 ctgagaacat aagaacttcc taagtttccg ctcaaacccc gctctcattt gagagcggcg
     1381 catgagggtt gaagtgtggt gttttgaaac aaaacctgat ggatagaaca cccatcaggt
     1441 catttggtta gttcgctgat gcctgttcag gcactgcctc ttcgtacttt gtggggcatt
     1501 tgagcaaaag ctcctcagca tggaaaacgc cgtcatcacc cagctttccg gtcatgatcg
     1561 cctgcgcttc gttacggagc aggtcgggca tcacgccttc atactgcacc ttgatgcgtg
     1621 cccggctcgt gtccatcacc gcttcgtgaa gcacagcagc caaaccaccc tgggcttcga
     1681 tctcggcatt atcgccagga acgtgtgcca cttcaaaggt caaggtcagc gttgaggcat
     1741 cgtattggat cgtatctcca atgaccgcgc ctgaaagacg gacactcttg ttgatggcag
     1801 aggaaccttc tgcattaagt tcatccaccg tcatgaaata ttctgcgctc gcctgtgtgg
     1861 acgaggcgat gagataaacg acagccgcca gaatgagcac gccgccgatg ataaatttgt
     1921 ttcgttgcat aggaggtctc caagggttgt gttactaaat caaacaatct atgtccattt
     1981 tacatgatga cattaaatag g
//
LOCUS       DAOQNJ010000520         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_48558_length_1994_cov_6.706034, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000520 DAOQNJ010000000
VERSION     DAOQNJ010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48558_length_1994_cov_6.706034"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLG52_12445"
     CDS             complement(<1..389)
                     /locus_tag="PLG52_12445"
                     /inference="COORDINATES: protein motif:HMM:NF021459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2172 domain-containing protein"
                     /protein_id="HNB42305.1"
                     /translation="MTNRMKPYNSLKSILAEFFPLHRTLVSDDHDKTLEIVGSYMPES
                     SYYTIETYAPLKQVWTWKVPERYVVHEAYLEIEGGERIVDFKDNPLHIVSYSLPVDKV
                     LNFDELQPHLYFNEKLPHTVPWVFKYY"
     gene            complement(459..1229)
                     /locus_tag="PLG52_12450"
     CDS             complement(459..1229)
                     /locus_tag="PLG52_12450"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNB42306.1"
                     /translation="MLSKKELLDGFGQLNLKKGETIIVHTSYKSLGGVEGGADTVIDV
                     MSELVGKDGTVLFPAFNFQSWTENHYFDVKETPSKMGAITEQARLRPNALRTPHPIYS
                     FSALGARAEEFSKTEDVEAYGPNSAFALFHKINGTIVSIGLDFNSTFSMHHYIEYNVG
                     CNYRRIKEFSGIYVGYDRVPKLKMHSMFVRNDDTVKTYINPGMDELLAAGVIKEVQVG
                     AAKIHFATANDFYDNMSVIVREHPEKLHIIEEPKYRKF"
     gene            complement(1338..>1994)
                     /locus_tag="PLG52_12455"
     CDS             complement(1338..>1994)
                     /locus_tag="PLG52_12455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB42307.1"
                     /translation="NVTKDKAVAYGMNPEKTIVFPWGVDLDHFTKKEEIDSKEGFVLF
                     CNRSWETRYGVDVLARAFVKVASQREDIRLLLLGGGSQGAYLRKIFQSGGVDEYVTYG
                     GQISQTDLPRWYHMADLYISPSHVDGSSVSLMEALACGLPCLVSDIPANKEWVFENQN
                     GWLFRDGDVDHLAEKILAAMNQREKLPEIGRASRSEAEKRADWKKNAAALMEVYRSLK
                     "
BASE COUNT          501 a          545 c          434 g          514 t
ORIGIN      
        1 tcgtaatact tgaagaccca cggcacggtg tgcggaagtt tttcattgaa atacaaatgc
       61 ggttgaagtt cgtcgaaatt caaaacttta tcaacaggca gtgaatacga aacgatgtgc
      121 aacggattat ctttgaagtc cacgatccgc tcgccgcctt cgatctccag atacgcctcg
      181 tggacgacat agcgctccgg cactttccat gtccaaacct gcttgagcgg tgcatacgtt
      241 tcaatggtgt agtacgaaga ctcgggcatg tacgagccga cgatctccaa cgtcttgtca
      301 tggtcatcag agacgagagt tcgatgcaga ggaaagaatt ccgcgaggat agatttgagg
      361 gagttgtagg gtttcattcg atttgtcacg taggggcgag gtctcctcgc tccgttttat
      421 aacacacata aagggcgggg agaccccgcc cctacaaatt aaaacttacg atattttggc
      481 tcttcaatga tatgcagttt ctcaggatgt tcacgcacaa tgaccgacat attatcgtag
      541 aaatcatttg ccgtcgcaaa atggatcttc gctgcgccga cctgcacttc tttgattacg
      601 cctgccgcta aaagttcatc catgccaggg ttaatataag tcttcactgt gtcatcgttg
      661 cgcacgaaca tcgaatgcat ttttagtttc ggcacgcgat catagccaac gtaaatgcca
      721 gagaactcct tgatgcggcg ataattacag ccaacgttat attcgatgta atgatgcatc
      781 gagaaagtgc tattgaaatc caagccaata cttacaatgg ttccgttgat cttatggaac
      841 aatgcaaacg ccgaattcgg accgtaggct tccacatctt cggtctttga aaactcttcc
      901 gctcgggcac ccaaggcaga aaaactatag atggggtgcg gagtccgcaa ggcgttcggg
      961 cgcaagcgcg cctgctccgt gatcgcgccc atcttcgacg gcgtttcttt cacatcgaaa
     1021 taatggttct ccgtccacga ttggaagttg aacgccggga acagcaccgt gccatccttg
     1081 cccaccaatt cactcatcac atcaatgacc gtgtccgcgc cgccttccac cccgcccaac
     1141 gatttatacg acgtatgcac aatgatcgtc tcgccttttt tcaagttgag ttgaccaaaa
     1201 ccatccaata attctttttt acttaacata ttttcctttt cccgtcattg cgaggagggc
     1261 gacagcttga cgaagcaatc ttgtactcaa ttatgagatt gcttcgggcg aaagaacatc
     1321 gccctcgcaa tgacgtatca ttttagactt cgatagactt ccatcagcgc cgcggcattc
     1381 tttttccaat ccgcgcgctt ctctgcttca cttcggcttg ctctgccgat ctcaggcagt
     1441 ttctcacgct gattcattgc cgccagaatc ttctctgcaa ggtgatccac atccccatcg
     1501 cggaacagcc agccgttctg attctcgaat acccattctt tattggcagg gatatccgac
     1561 acgagacaag gcaacccaca cgccaaggct tccatcaacg acacagatga accgtccaca
     1621 tgcgaaggtg aaatgtacag gtctgccata tgataccagc gcggaagatc cgtctgcgag
     1681 atctgcccgc cataggtgac gtattcgtcc acaccgccgc tttgaaaaat cttgcgaaga
     1741 taggctccct gcgacccgcc gcctaaaagc aataaccgta tatcctccct ctggcttgcc
     1801 actttaacga acgcgcgcgc cagcacatcc accccgtaac gtgtctccca ggagcggttg
     1861 caaaatagaa cgaaaccttc tttactatct atttcttctt tctttgtgaa atgatccaag
     1921 tccacacccc aagggaagac gatggtcttt tctggattca tgccatacgc aacggcttta
     1981 tctttagtca catt
//
LOCUS       DAOQNJ010000521         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_48640_length_1992_cov_7.351575, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000521 DAOQNJ010000000
VERSION     DAOQNJ010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48640_length_1992_cov_7.351575"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1054)
                     /locus_tag="PLG52_12460"
     CDS             complement(167..1054)
                     /locus_tag="PLG52_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB42308.1"
                     /translation="MPKSKVIPYLEALFAVLVWGGTFIATKIALSEVSPATIVWIRFG
                     MGTVILGIVVVARKQFALPEKSEWLYFAMLGFLGVTFHQILQATGLQTAKATTTAWIV
                     ATTPVFIAILGWVALKEKLNGLQILGIVLAGFGVLLIVSKGNIGALFTGEEGTFGDFL
                     VLISSVNWAVYTILSRRELARHPAARMMFFVMLIGWLLVNVWIFGFGPGVSEIPQLTS
                     SGWVAILILGIFGSGLAYIAFYDALQEIPASQLGAFFNVEPLVTTILASFMINEAITA
                     ITLIGGAIIILGIWLVNRK"
     gene            1285..1932
                     /locus_tag="PLG52_12465"
     CDS             1285..1932
                     /locus_tag="PLG52_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB42309.1"
                     /translation="MEITWYGHSCFRLTERNYATVVTDPFDHKAVGYSALKLKADIVT
                     VSHDAPGHANLEAVKGSEHVIDGAGEFEIGGVFITAVQTAGGGKKSKDKTRNTLYVFD
                     YEGITVAHLGDLQEVPTQSEVEALGTVNILLIPVGGGSSLNAAKAAEVVSVIEPNLVI
                     PMHYSTDAAKIKLDPLNKFLKEMGLGKVESLPSLKATRSSLPQETKVVVLDYQQG"
BASE COUNT          557 a          470 c          505 g          460 t
ORIGIN      
        1 ggtcagagca tgatgcggga attttttccg aggtagacca aagtaatcat cgatctgtgc
       61 aaaacgatag gcagacgaat ctccggctgg gattttcatc aaatagcctt tttcggtctt
      121 tgagatgcta gcttcagggg tagtgcgtgc ttttaatggc atttgattat ttccgattta
      181 ccaaccaaat acctaatatg ataattgcgc cgccgatgag agtgattgcc gtgatggctt
      241 cgttgatcat gaatgaagcc agaatggtag tgaccaacgg ttcaacattg aagaatgccc
      301 caagttgtga ggcgggaatt tcttgcaagg catcatagaa tgcaatgtac gccaaccctg
      361 aaccgaagat accaagaatc agaatggcaa cccagcccga agacgtgagc tgcggaattt
      421 cactcacacc cggaccaaaa ccaaaaatcc acacattgac caaaagccag ccgatcagca
      481 tgacgaagaa catcatccgt gccgcagggt gacgtgctag ctcacggcgc gagaggatgg
      541 tgtacaccgc ccagttgacc gagctgatga ggacgaggaa atcaccgaag gtcccttctt
      601 cgccagtgaa aagcgcaccg atattgccct tactgacgat gagcaaaacc ccaaacccgg
      661 caagaacaat accgagaatt tgcaacccgt tcagtttttc tttgagtgct acccaaccga
      721 gaatggcaat gaaaacgggc gtggttgcca cgatccatgc agtggtggtg gctttggcgg
      781 tttgtagccc cgtagcttgc aggatttgat gaaaggtgac acccagaaaa ccgagcatgg
      841 caaaatatag ccactcgctt ttttcaggca gggcaaattg cttgcgggcg acaacgacaa
      901 taccgaggat gactgttccc atcccaaaac ggatccacac gatggtagca ggggagactt
      961 cgctgagcgc gatcttggtg gcgataaatg tcccacccca aacgaggacg gcaaataaag
     1021 cttcgagata gggaatgact ttagattttg gcataaggtt tcctgtggaa agagttggag
     1081 aattataatt gttgtttact acactacttt aaggggtatt tttcctaagc ggaattcacc
     1141 atttgggtag ggattttgaa ggtgttgtga tggtcgggaa atagtcccac caaaaactaa
     1201 gacctatttg actattagac gaccaaactt cattttgtcc gtgtacccat cgggtataat
     1261 atctccctcg gaggcactag ctacatggaa attacatggt atggacattc ttgttttaga
     1321 ctgacagaac gcaattacgc gactgttgtc accgacccgt tcgatcacaa ggcggtgggc
     1381 tacagtgcgc tcaaactaaa agcggatatt gtcaccgtca gccacgatgc acccggtcac
     1441 gccaatttag aggcagtgaa gggtagtgag catgtcatcg acggcgcggg cgaatttgag
     1501 atcggcggtg tatttattac agcggttcaa actgccggag gcggcaagaa aagtaaggat
     1561 aagacccgca acaccctcta tgtgttcgat tacgaaggta tcactgtggc acatctcggc
     1621 gacttgcagg aagtgcccac tcaaagcgaa gtagaagcct tgggaacagt gaacattctc
     1681 ctcatcccag ttggaggcgg aagcagtctc aatgccgcca aagctgcgga agtggtcagc
     1741 gttatcgagc cgaatcttgt catccccatg cattactcca ccgacgccgc caagatcaag
     1801 ctcgacccat tgaacaaatt tctcaaagaa atgggtctcg gcaaggttga gtctctgcct
     1861 tctttgaaag ccacccgttc ttcattaccg caagaaacca aggtggttgt gctcgattac
     1921 caacaaggat agtatggcag ttaaagtcat catgacctgg gatatcgccg ctgaacgcga
     1981 ccaggaatat tt
//
LOCUS       DAOQNJ010000522         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_48823_length_1988_cov_9.245215, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000522 DAOQNJ010000000
VERSION     DAOQNJ010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48823_length_1988_cov_9.245215"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="PLG52_12470"
     CDS             complement(<1..1458)
                     /locus_tag="PLG52_12470"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNB42310.1"
                     /translation="MHLSETVNTGFYFLAPGLVLAPIIGLLINTIFGKRFSETIVGAI
                     ASTFSGIAFVISVLLAYSVAINHGHAVSVPFAEWIHIGNLKLDWTFRVDSLSATMMLV
                     VSGVGTLIHIYAIGYMHEDVRFKHEEGRFNRFFIFLNLFIAAMMILVSGDSYLMLFVG
                     WEGVGLCSFLLIGFWYEMDTLARPSWANSNAAKKAFITNRVGDFGFLMAGFLMFWHLG
                     GSFQFDEVFKAAPAIAEAQPWIIVAITLFMLVGVAGKSAQIPLYIWLPDAMAGPTPVS
                     ALIHAATMVTAGVYLVTRSAPLYTLVPQAQYIVAMVGATTALFAATIAVGQYDIKKVL
                     AYSTISQLGFMVAAVGMGAYVAGMFHLITHAFFKALLFLSAGSVILGLERGHHAHAHH
                     AAHEEKPKGKHKKEEHGHGGHDDHGEVFDPGDMRNMGGLRKTMPVTFWLYIIGTLALA
                     GIFPFAGFWSKDEILLDASLHFTSVYWQLTIAAFFT"
     gene            complement(1578..1880)
                     /gene="nuoK"
                     /locus_tag="PLG52_12475"
     CDS             complement(1578..1880)
                     /gene="nuoK"
                     /locus_tag="PLG52_12475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNB42311.1"
                     /translation="MVPVDYYIVLSAILFTIGALGVLSRRNPLIIFMSIELMLNAANL
                     AFVAFARVYDTFSGQIFVFFVIAVAAAEVAVGLALIVEIFKSKKNINIDEMNSLKG"
BASE COUNT          552 a          502 c          523 g          411 t
ORIGIN      
        1 ggtgaagaac gcggcaatcg tcaactgcca gtaaacactg gtgaagtgta ggctggcatc
       61 gagcagaatt tcatctttag accaaaagcc tgcaaatggg aaaatgcccg ctaatgccaa
      121 cgtgccgatt atgtacagcc aaaacgtaac cggcatggtc ttgcgaagcc cgcccatatt
      181 acgcatatcg ccggggtcga agacttcgcc gtggtcatca tgaccgccat gtccatgttc
      241 ttctttctta tgtttgcctt ttggtttctc ctcatgagcg gcgtgatgcg cgtgtgcatg
      301 atgtccgcgc tccaagccga ggatgacgga accagccgag aggaagagca acgccttgaa
      361 gaaggcatgt gtaatgagat ggaacatccc cgctacataa gcgcccatgc ccacagccgc
      421 aaccatgaag ccgagctgtg aaatggtgga gtacgcgagg acttttttga tatcgtactg
      481 ccccaccgca atcgtcgcgg cgaaaagtgc agttgtggca ccgaccatgg caacaatata
      541 ttgcgcctga ggaaccaacg tataaagcgg tgccgaacga gtgacaagat acacacccgc
      601 ggtcaccatt gttgcggcat gaatgagggc cgagaccggg gtcggacctg ccatcgcatc
      661 gggcaaccag atgtaaagag gaatttgtgc agatttacca gctacaccca ccaacatgaa
      721 gagggtaatg gcaacgatga tccatggttg agcctctgca atggcaggcg ctgctttgaa
      781 gacttcatcg aactggaagg aaccgccaag atgccagaac atcaagaagc cagccatgag
      841 gaagccaaag tcaccgacac ggttggtgat gaaggctttc ttcgcggcgt tcgaatttgc
      901 ccaggatgga cgggcaaggg tatccatctc ataccagaag ccgataagca ggaaggagca
      961 caagcccacg ccttcccagc cgacgaagag cattaggtag gagtcgccag agacaaggat
     1021 catcatcgca gcgatgaaca ggttcaagaa aatgaagaag cggttgaaac gtccctcttc
     1081 atgtttgaag cgtacatcct cgtgcatgta tccaatggcg tagatgtgaa tgagagtccc
     1141 aacgccagaa accaccagca tcatcgtcgc tgataacgag tcgacgcgga aggtccagtc
     1201 gagtttgagg ttgccgatgt ggatccattc ggcaaagggc acgctcacag catgaccgtg
     1261 attgatcgcc accgaatatg ccagcagcac tgagatgacg aatgcaatac ctgaaaatgt
     1321 acttgcaatc gcgccaacga tggtctcgga aaaacgtttt ccgaatatgg tgttgatgag
     1381 caaaccgatt atcggcgcca gcaccaaccc gggggctaga aaataaaacc ctgtgtttac
     1441 tgtttcactt aagtgcataa cattctccag agtttcccgt cattgcgagg gcgatgttct
     1501 tttgcccgaa gcaatctcat gattaagtct aagattgctt cgtcgggaag agcaccctcc
     1561 tcgcaatgac gaagcctcta tccttttaga gaattcatct catcgatgtt gatgttcttc
     1621 ttggacttga aaatttccac gatcaacgcg agacccacgg ctacttccgc agcggccacg
     1681 gcgatgacaa agaagacgaa gatctgtcca ctgaacgtat cgtacacccg cgcaaaggcg
     1741 acaaaagcca ggttggccgc gttaagcatc agttcaatgg acatgaaaat gatgagcggg
     1801 ttgcggcgcg agagcacgcc caacgcaccg atggtgaata gaatggcgga aagaacgatg
     1861 taataatcaa caggcaccat tagctttgtg ctcccttttc ctgcttggtc aacacgatcg
     1921 caccgaccat tgcaaccaac agcagaatgg atgtcacttc aaagggtaga aggaattgat
     1981 tgaagagg
//
LOCUS       DAOQNJ010000523         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_48954_length_1985_cov_7.189637, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000523 DAOQNJ010000000
VERSION     DAOQNJ010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48954_length_1985_cov_7.189637"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1010
                     /locus_tag="PLG52_12480"
     CDS             24..1010
                     /locus_tag="PLG52_12480"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB42312.1"
                     /translation="MKTLITGATGFLGGALAQRLHGMGWNVTALGRNPKKLNGLEDSG
                     MHVVRADISKKDEVTASFSDFDLVFHCAAFPSPWGSFEKFYQANVIGTRNIVQACLEN
                     KVKRLVYVSTPSIYFDYNSRVNVKENDVLPEPISHYAHTKSLAEEEVDKGFQNGLAVI
                     SIRPRALFGEEDTVIFPRLLARLKSGRLPILGDGENVVDLTYIQNVVDALLLCAEAPE
                     ASLGKKYNISNGEPVKIWSLINQICDELGFSRPSRHISRQTAHMAGGTLEFLYSLIPY
                     SPEPPLTRMSVSMLSNSTTLDISAAKAELGYQPKVSVEEGVQRFLKWWKEKN"
     gene            1081..1920
                     /locus_tag="PLG52_12485"
     CDS             1081..1920
                     /locus_tag="PLG52_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB42313.1"
                     /translation="MKIDILHAGYCTSPEHLAIQGGRWRTIHFPAMFTLLRHPKFGAM
                     LFDTGYSYRFFEETKKFPMRFYRWMTPVHLNEEDLVVNQLATFNLRPTDISFVFISHF
                     HADHVGSLCDLPKSRYVYLPHAYSHLRSLSPSKDMKHAFLRGQIPSDFDARSQEVDMS
                     RSILLSEEYAPFKTGYDLLGDGSIIGVELPGHAHGQMGIFARNDEDKIFFFVADAAWL
                     KRSIVENRPPHKIADMLFPDPAAYRETLGKLQTYTLTHPDTVVVPSHCDETISQLSTS
                     GHK"
BASE COUNT          507 a          499 c          451 g          528 t
ORIGIN      
        1 ttaattacgg atacaatcga tatatgaaaa ccctcatcac tggtgcaacc ggatttttag
       61 gcggagcatt ggcgcaacgc ctgcatggca tggggtggaa cgtcaccgcc cttggacgta
      121 accctaaaaa gttaaatgga cttgaagaca gcggcatgca tgttgttcgt gctgatatct
      181 ccaaaaagga tgaagtgacc gcctctttct cagactttga cctggtcttt cactgtgcgg
      241 catttccctc gccttggggt agcttcgaaa aattctatca agcgaatgtg attggcaccc
      301 gcaacatcgt gcaggcttgc cttgaaaaca aggtcaagcg cttggtttac gtctctacgc
      361 caagcatata ttttgactac aactcacgtg taaacgtcaa agagaatgac gttttgcccg
      421 agcccatcag ccattatgcg cacacaaaat cactcgctga agaagaagta gacaaaggct
      481 tccaaaacgg actggctgtg atctccattc gtccgcgggc gctttttgga gaagaagata
      541 cggtcatctt cccacggctt ttggcaagac tcaaaagcgg caggctgccg atccttggag
      601 atggcgagaa tgtcgttgac ctgacctata tccaaaatgt agtggatgca ttgctgcttt
      661 gtgccgaagc accagaggct tctctgggta aaaaatataa tatttcaaat ggcgaaccgg
      721 tgaaaatatg gagcctgatc aaccagatat gtgatgagct tggcttttca cgtcccagcc
      781 gccatatctc ccgccagacc gcccacatgg caggtggcac acttgagttc ttgtattccc
      841 taatacctta cagccctgag ccaccgttga ctcgcatgtc tgtaagtatg ttgtcgaata
      901 gcaccacact cgatatttct gctgcgaagg cagaactcgg ctatcagcca aaggtttcgg
      961 tggaagaagg cgtgcaacgg tttttgaaat ggtggaaaga aaagaattaa ccacaaaggt
     1021 cacaaagtac accaaggttt actttgtgac ctttgtgtac tttgtggtta aaaggctctt
     1081 atgaaaatcg acattcttca cgcaggctac tgtacctctc ccgagcatct cgccattcag
     1141 ggcggacgct ggcgcacgat ccatttccct gccatgttca ctcttttgcg tcatcccaaa
     1201 ttcggcgcaa tgcttttcga cactggctat tcttatcgct tcttcgaaga gacgaagaaa
     1261 ttccctatgc gtttttaccg ctggatgacc cctgttcatc tcaatgaaga agacctcgta
     1321 gtcaatcaac ttgcaacctt caaccttcga cctacggaca tatctttcgt ctttatttct
     1381 cacttccatg ccgatcacgt tggctcgctt tgtgatttgc caaagtctcg ttatgtgtat
     1441 ttgccgcatg cctattcaca tttgcgcagc ttatcgccat ctaaagatat gaaacacgcc
     1501 ttcctgcgcg gacagatccc ctcagacttt gacgcccgct cgcaagaagt agatatgagc
     1561 agatccattt tgctttcaga agaatatgcg ccctttaaga ctggctatga cctgctcgga
     1621 gacggctcca tcatcggcgt ggagttgccc ggtcacgcac atggacagat gggaatattt
     1681 gctcggaatg acgaagataa gatcttcttt ttcgtggctg atgctgcttg gttaaagcgt
     1741 tccattgtgg agaaccgtcc gccacacaag atagcggata tgctcttccc cgacccggct
     1801 gcttaccgtg aaacgttggg aaaattgcaa acgtatacac taacccatcc cgatacggtg
     1861 gttgtcccgt cccactgtga tgagacgata tcacaactca gtacatcagg tcataaatga
     1921 acatcaaaac taaagtaact gttataggcg tgataagctt tttggtcctt gcttgtaatc
     1981 taggt
//
LOCUS       DAOQNJ010000524         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_49393_length_1976_cov_9.708485, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000524 DAOQNJ010000000
VERSION     DAOQNJ010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49393_length_1976_cov_9.708485"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLG52_12490"
     CDS             <1..843
                     /locus_tag="PLG52_12490"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNB42314.1"
                     /translation="SLNIGMDMDSFENVMRVLARFRSHIAAHPNELILARSVEDIHRA
                     KRENKLAIAFDLEGSNPLCGDLNLVSFYYDLGVRQMLLAYNKDNAASGGCMEGRIGLT
                     EFGRQVVQEMNRVGMVVDVTHMGYRATMDTFEVSTKPVIFSHSNPARLHAHARNISDE
                     QIKACAKTGGVIAINGIGDFLGGKTSELFVQHLEYVLNLVGPEHVGIGLDYVIDKQEL
                     IDYIQGNPDVFPADRFEDFLSFVEPEQLPEISEILYKHGHDEDVIRKVLGGNFLRVAE
                     QTWK"
     gene            871..>1976
                     /locus_tag="PLG52_12495"
     CDS             871..>1976
                     /locus_tag="PLG52_12495"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNB42315.1"
                     /translation="MSTILLTGGRAPVTLDLARAFHRAGHVVYVAESLRGHLTEPSAA
                     IKAHFEVPAPRQEPEAFLAALRSIIEAYQIELLIPTCEEVFHIAKGLNTLPCRVFTEP
                     LARLDGYHNKWKFVVKAGECDLRVPESMLVTRQDDLLYAYAHWRGLVLKPVYSRFAAR
                     TLILPPLKDAMATLTFDSLTNQEPWIAQEYIEGEQFCSYSVCQNGRILAHAVYPTAFT
                     AGQGATIAFQSVQHDAIYEWVRTFVEHYKVTGQMAFDFVQTADGNVVALECNPRATSG
                     AHLLTSNPRFVESFFNSELECITPYEGTSRMLGTAMLTYGLPASLFNGRFGAWLKTFS
                     HSDDVLLDFRDPLPFLLQWRSILSYVLLGRKQGIS"
BASE COUNT          454 a          475 c          549 g          498 t
ORIGIN      
        1 tcgctgaaca tcggcatgga catggactcg ttcgagaatg tgatgcgcgt gttggcacgt
       61 tttcgctccc atattgcggc tcatcccaat gaattgattc tggcacggtc tgtggaagat
      121 attcaccgcg ccaaacgaga gaataaatta gccatcgctt ttgatctgga agggtcgaac
      181 ccgttatgcg gtgacctgaa cctagtctcg ttctattatg atcttggtgt gcgccaaatg
      241 ctattggcat acaacaaaga caatgccgct tcaggcggat gcatggaggg gagaatcggg
      301 ctgacggagt tcggcaggca agtggtgcaa gagatgaacc gtgtgggcat ggtggtggac
      361 gtaacgcata tgggctaccg cgccacaatg gacacctttg aagtttctac gaaaccggtc
      421 attttttcac attcaaaccc tgcccgtttg cacgcccatg cccgaaatat ttcagatgag
      481 cagatcaaag catgtgccaa gaccggcggc gtgatcgcca tcaacggcat tggcgatttt
      541 ttaggcggca aaaccagcga gctttttgta caacaccttg agtatgtttt gaatttggtc
      601 ggacccgaac atgtgggaat tggattggat tatgtcatcg acaaacagga acttatcgac
      661 tacattcagg gaaatccgga tgtcttccca gccgaccgct ttgaagattt cctttcgttc
      721 gtagaaccag aacaattgcc agagatcagc gagattttat acaagcacgg gcatgatgaa
      781 gatgtgatcc gtaaggtgtt gggtgggaac ttcttgcggg tggcagaaca aacctggaag
      841 taaaaaatcc cttattggca ggctgaccta atgtcaacca ttcttctcac cggcgggcga
      901 gcacccgtca ccttagatct ggcgcgtgcg tttcaccgag cggggcatgt agtgtatgtt
      961 gccgagtctt tacgcgggca tctcaccgag ccttcggcgg cgatcaaggc tcattttgag
     1021 gtgccagctc cacgtcagga acctgaggcg tttttagcgg cgttgcgaag catcatcgaa
     1081 gcgtatcaga tcgagttgct gattcccacc tgtgaggaag ttttccatat tgcaaaaggg
     1141 ttgaatactt taccctgccg cgtgtttacc gagccgctgg caaggttgga tggatatcac
     1201 aacaagtgga agtttgtggt gaaggcgggg gaatgtgatt tgcgcgtgcc tgagtcgatg
     1261 ttggtgacga gacaagatga cttgctctat gcctacgctc actggcgcgg actggtgctg
     1321 aagccggtct attcccgctt cgctgcgcgg accttgatct tgcccccgtt aaaagatgcc
     1381 atggctacgc tgacgtttga ctctctcacg aaccaagagc cgtggatcgc acaagaatat
     1441 attgagggag agcaattttg tagttattcg gtttgtcaga atggacgcat tctagcgcat
     1501 gcggtctacc ccacggcgtt cacagccggg cagggagcaa cgattgcgtt tcaatcggtg
     1561 cagcatgatg ccatctatga atgggtgcgg acctttgtgg agcattacaa agtgaccggg
     1621 caaatggcgt tcgattttgt gcaaacagca gatggcaacg tggtggcgtt ggaatgtaac
     1681 ccacgtgcca caagcggagc gcatttgctg acctcgaacc cgcgctttgt agagtctttc
     1741 tttaacagtg aactggagtg cattacaccc tacgaaggga cttcgcgtat gttgggcaca
     1801 gccatgttga cctatgggct gcccgcctcg ctatttaacg gtcgctttgg ggcgtggcta
     1861 aaaaccttct ctcacagcga cgatgtgctc ttagactttc gcgacccatt gcccttcctt
     1921 ctgcaatggc gcagcatcct ttcatatgtg ttgctgggtc gcaagcaagg catctc
//
LOCUS       DAOQNJ010000525         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_49547_length_1973_cov_14.447341, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000525 DAOQNJ010000000
VERSION     DAOQNJ010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49547_length_1973_cov_14.447341"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..770
                     /locus_tag="PLG52_12500"
     CDS             81..770
                     /locus_tag="PLG52_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HNB42316.1"
                     /translation="MHKLESIADQIRKNFDARTAARDQALAQARQLTRACSLAIRAVH
                     RDDTESMQAQLLEAHRLADTLRNSLVSYPDLFHAGYTQDALKEFVEANVTCALIKSQP
                     LPTPEELGVEPATYLNGLAEVVGELRRRTMDILRHGYSAEAERLMGVMDEIYSVLVTM
                     DYPDAITNGLRRQTDLARGIIEKTRGDITFSLRGGHLEKAIGRLLKQMNGEQAQGPEG
                     SEAFPPMDEDD"
     gene            773..1390
                     /locus_tag="PLG52_12505"
     CDS             773..1390
                     /locus_tag="PLG52_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42317.1"
                     /translation="MAAKKGGRRYPLVIFTHMMSRWWTAVFTLGLAMLGLAWAIRSWG
                     FEQWRWMTFAVLGGFTALAGILMWLFRKSAYVQPFADHLKLVTPFLRMNISYKRFRRT
                     STATMGGIFPPKSLPRSLQEIVEPIAKMSAIVIEVSALPMSQNALRLFLSPLFFKDKS
                     PHIIILVSDWMRFSAEMDSMRHGGADVPNVAHRRDISILSKLPKK"
     gene            1390..1800
                     /locus_tag="PLG52_12510"
     CDS             1390..1800
                     /locus_tag="PLG52_12510"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNB42318.1"
                     /translation="MNIYFACSITGGRELESFYQQFVAALEADGHVIPTSHLAQSEAM
                     EGERMLTPVDVYERDVKWVRECDVLIAEVGVPSHGVGYEIGYALNAGKPVLCLAQNGM
                     RVSKMITGNSALEMKTYSTLEEAITLARNFLSSI"
BASE COUNT          472 a          479 c          499 g          523 t
ORIGIN      
        1 catgtgcctg tcaactcttc tctggcagat aatctcttcc gcccgttcca acagccgcct
       61 gaaccgccta aagaataacc atgcacaaac ttgaatccat cgccgatcaa attcgcaaga
      121 attttgatgc tcgtactgcc gcacgtgacc aagcattggc tcaggcgcgt cagttaaccc
      181 gcgcttgttc acttgcgatc cgtgcggtac accgtgacga taccgaaagc atgcaagccc
      241 aacttctcga agcccatcgt ctggcagaca cccttcgcaa ttctctcgtc tcatacccag
      301 acctcttcca tgctggatat acccaagacg cgctcaaaga atttgtggaa gccaatgtga
      361 cctgtgcatt gatcaaaagc cagccgcttc cgactccgga agaattgggt gttgaacccg
      421 caacctatct caacgggttg gcagaagtgg tcggtgaatt gcgccgccgc acgatggata
      481 ttctccgtca cgggtattcg gcagaagccg aacgcttgat gggcgtgatg gacgaaatct
      541 actctgtgct tgtcaccatg gattatcccg acgccatcac gaacggactt cgccgtcaga
      601 ccgatcttgc tcgtggcatt atcgaaaaga cgcgcggaga tatcacattc agtttgcgtg
      661 gcgggcattt agaaaaagcc attgggcgtt tgctcaagca aatgaatggc gagcaggctc
      721 aaggtccaga aggaagcgaa gcatttcctc ccatggatga ggatgattaa ccatggcagc
      781 aaaaaaaggc ggacgccgct atcctcttgt catttttaca cacatgatga gccgttggtg
      841 gacggcggtc tttactcttg gcttggcgat gcttgggctg gcgtgggcaa ttcgtagttg
      901 gggctttgag caatggcgtt ggatgacttt tgcggtgttg ggtggtttta ctgcattggc
      961 aggaattctg atgtggctct ttcgcaagag tgcgtatgtg caaccatttg cagatcatct
     1021 aaaactggtg acgccatttt tgcgaatgaa tatttcgtat aaaagatttc gtcgcaccag
     1081 cactgccacc atgggcggaa ttttcccgcc caaaagcttg ccgcggtctc tgcaggaaat
     1141 cgttgaaccc attgcaaaga tgagcgctat tgtgatcgaa gtgagtgctt tgccaatgtc
     1201 gcaaaatgcg ttgaggcttt tcctttcgcc gcttttcttc aaagataaat ctccgcatat
     1261 catcatcctt gtttcagact ggatgcgttt cagcgctgag atggatagta tgcgtcacgg
     1321 cggggcggat gtgcccaatg ttgcacatcg tcgcgacatt tccattcttt caaaactccc
     1381 gaagaaataa tgaatattta ttttgcatgt tctatcacgg gtgggcgtga gttggaatct
     1441 ttttatcaac aatttgttgc cgcccttgaa gccgatgggc atgtgatccc tacttcgcat
     1501 ctggctcaat ctgaggcgat ggaaggcgag cgtatgctta ctccggtaga tgtgtatgaa
     1561 cgcgatgtga agtgggttcg agaatgtgat gttttgatcg cagaagtggg agtcccttcc
     1621 catggcgtgg gctatgagat cgggtatgcc ttgaatgctg gcaaacctgt gctttgcttg
     1681 gcacaaaatg gaatgcgtgt ttctaaaatg atcactggga attctgccct tgaaatgaag
     1741 acatattcca cccttgaaga ggcaattact ctggcacgta atttcttgag cagcatctga
     1801 tcttatgtgc ttcatcatgt gcaatgggtg acatattacg atgcccaaaa aacggaacct
     1861 gcattaatat cactcacgga gacggttgta gacacctctc aacgcagaat tccagtctcc
     1921 tcctttggcg aaagctgacg tcaggtgaac agcctgacgt cagcatttta att
//
LOCUS       DAOQNJ010000526         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_49601_length_1972_cov_7.898279, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000526 DAOQNJ010000000
VERSION     DAOQNJ010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49601_length_1972_cov_7.898279"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLG52_12515"
     CDS             <1..448
                     /locus_tag="PLG52_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42319.1"
                     /translation="IGERVKTLTGWLLENLTAMKHSTSVPLVRLFGPNSTEGRGGAVT
                     VNFYDKNGMAFDHRFIESEANKVNISLRTGCFCNPGAGEIALGISRMELDVCFTRPEH
                     KDVMSIDEFRKCIDGKSSGAVRISVGPFTNFNDIQSLLMFARGLLS"
     gene            751..1146
                     /locus_tag="PLG52_12520"
     CDS             751..1146
                     /locus_tag="PLG52_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42320.1"
                     /translation="MASQIQALNHYRPQIEYGETAGWREVAEFLEARTTLSKTDIIAT
                     LTGLQDAVLYFNLQGRGVKLEGLGSYLPNINYQGEIDAAHRLDRRLKRGLNNGSFSGK
                     IRNKKNIGKTVEQVVAMWNAEHPDDPVLY"
     gene            1391..1891
                     /locus_tag="PLG52_12525"
     CDS             1391..1891
                     /locus_tag="PLG52_12525"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB42321.1"
                     /translation="MNISEIHLMFEYNYWANGRIFNAAKPITSEQLLAPAEFPYGDLR
                     ATLVHIIDAEFGWRGLFENNKFNEEIKPEEFGDVQSIHERAQIEEQAMRAYINRLTDA
                     DMESHKKYIADNGEPRDRILWHCLYHVVNHGSQHRSEAAAILTHFNASPGDLDFTVFL
                     NEIKPK"
BASE COUNT          551 a          481 c          480 g          460 t
ORIGIN      
        1 cattggagag cgggtcaaaa cattaaccgg ctggctgttg gaaaacctca ccgccatgaa
       61 acacagcacc agcgtgccgt tggttcggtt gtttggtccc aactccaccg aaggcagagg
      121 cggagcggtc acggtcaatt tttacgacaa gaacggcatg gccttcgacc atcgctttat
      181 cgaaagcgaa gccaacaaag tgaatatctc gcttcgcacc ggctgcttct gcaaccctgg
      241 cgcgggtgag attgcccttg gcatttcacg tatggaattg gatgtctgct ttacccgccc
      301 cgagcataag gacgtcatgt ccatcgacga gttccgcaaa tgcatagacg gcaaatcctc
      361 cggtgcggta agaatctccg tgggtccctt tacgaatttc aacgacatcc agtctttgct
      421 gatgtttgca agagggttat tgagttaatc gtgtgctgag cggtagcgac acatgcaccg
      481 gagtgcggat gcagtgcggt gagcatctct acgataaccc gagagatcct tcggtcgcga
      541 agaacatgct ccctcaggat gacaagactg gttgcccttc agacctgcga ggtttttaaa
      601 acctcgcagg tcttgtttat aaagatgtcc tgtccaattg acaatatcct gccagcaaat
      661 ctataatacc ctacaaggaa ataagaaata tcctgacagg atattgggaa aatgtcgaaa
      721 cttgtttgta aacatatcag gaggaattac atggcgtctc aaattcaagc cctcaaccac
      781 taccgtccac aaattgaata tggcgaaacc gctggctggc gggaggttgc cgaattttta
      841 gaagcccgca cgacattaag caaaacggac attatcgcta ccctaacggg acttcaagat
      901 gccgtgctct acttcaatct tcaagggcgt ggggtcaagc tcgaagggct gggaagctac
      961 cttccgaata tcaactatca gggcgagatc gatgcggcgc accgtttgga taggagatta
     1021 aagcgtgggt tgaacaacgg ctcctttagc gggaaaatcc gaaacaagaa aaacatcggc
     1081 aaaaccgtgg agcaagtggt tgccatgtgg aacgccgagc atcctgatga ccccgtgcta
     1141 tactgaaaag gtacttaaat tagctcaagt tgctacctta tatactttag caggattggg
     1201 cagggttttt aacgtaaagt tgccaagacg ctaagagtta aaggcttttc ccttgcgacc
     1261 ttgcgccttt gcgttaaatg tttagatgca atccatacag gtagcatctc aatggcaacg
     1321 ccagagaccc ttcgccgaaa aagacaaaag tgcggctcac acgcaacaca atacacaaag
     1381 gagagacacc atgaacatct cagagattca cctcatgttt gaatacaact actgggcgaa
     1441 cggacgcatc ttcaacgccg cgaaacctat cacctcagaa caacttcttg ccccggcaga
     1501 atttccatat ggagatttac gcgccacgct cgttcacatc atagatgcgg agtttggttg
     1561 gcgcggtttg ttcgagaata ataaattcaa cgaagagatc aagccggaag aatttggaga
     1621 tgttcagtcg attcatgaac gtgctcagat cgaagagcaa gccatgcggg catatatcaa
     1681 caggcttacc gatgcagata tggaaagcca taagaaatat attgctgata atggcgagcc
     1741 acgtgaccgc atcttgtggc attgcttgta tcatgtcgtg aatcatggtt ctcaacatcg
     1801 cagtgaagcc gccgccattc tcacccattt caacgcttcc cccggcgacc tggactttac
     1861 ggtctttctg aatgaaataa agcccaaata aacactacgc cagcatggtg cgcatccggc
     1921 aaagggtcgg tatgatgcgg gtttatcatc caataaaagg agttttccaa tg
//
LOCUS       DAOQNJ010000527         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_50189_length_1959_cov_9.328256, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000527 DAOQNJ010000000
VERSION     DAOQNJ010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50189_length_1959_cov_9.328256"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLG52_12530"
     CDS             complement(<1..845)
                     /locus_tag="PLG52_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNB42322.1"
                     /translation="MNKKLAALLIFIFLTACAPLDIETPYTPVTQIPFYASDTPAPFI
                     VPVTETPQIIFPVTETPTLEQPVFVTDTPFPVAKWWEVYFTEPLTINDLNNPAGSIEE
                     KLINFINAAQFSIHIASFEFNLPRVADALIAAKARGVDVRWVTDDENGLEIDGNANRG
                     QFTRLMAAGVEVKDDAGRTALMHNKFWLFDRQITWTGSTNITVNGIYKQNNNVIVIHS
                     PEVTEIYEREWEELWTAQLGPRAPSTKNNQWAILDGTPLQILFSAEDDAVDSLIAILN
                     DAQVS"
     gene            complement(929..>1626)
                     /locus_tag="PLG52_12535"
     CDS             complement(929..>1626)
                     /locus_tag="PLG52_12535"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNB42323.1"
                     /translation="RLYPDLVPAALAGHSLGELSALTASGALSFEDGLRLVRKRGELM
                     KRAGELAPGSMAAILGVDIPTLDKVCAEASVTDELVQVANDNCPGQVVISGSKAAVER
                     AMAGAKAAGAKRALPLAVSIAAHSPLMASIQDEWNQAVDEASMHHPKTPVIGNVHASV
                     LKTAEEARADIKAQMQSRVKWTDSVKTISNMGVTAFVEVGTGSVLGGLVKRIVSDAVS
                     YPLGNPQDFSALE"
     assembly_gap    1627..1657
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1658..1864)
                     /locus_tag="PLG52_12540"
     CDS             complement(<1658..1864)
                     /locus_tag="PLG52_12540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HNB42324.1"
                     /translation="MKLNLNNTAFVFPGQGSQTVGMGKSLAEEQTIAKEVFAEADSIL
                     GFALSDLMWNGPDVELNDTVNTQPA"
BASE COUNT          491 a          484 c          455 g          498 t
ORIGIN      
        1 atgctcactt gggcatcgtt caaaatggca atcaggctat ccacggcgtc atcttcagca
       61 gagaaaagaa tctgcaatgg agtgccatct aagatcgccc actgattatt tttcgtagaa
      121 ggtgcccgcg gacccagttg tgccgtccac aattcttccc attcgcgctc gtaaatctcc
      181 gtcacttcgg gcgagtggat cacaatcaca ttattgtttt gcttgtaaat tccattgacg
      241 gtgatattcg tggagcctgt ccatgtgatt tgacggtcga acagccagaa tttgttatgc
      301 atcaaggcag tacgccccgc atcatccttg acttcgactc cggcagccat caaacgagtg
      361 aactgccccc gattcgcatt gccatctatt tccaacccgt tttcatcgtc tgtcacccaa
      421 cgcacgtcta caccgcgagc tttggcggcg atcaaagcat ccgctacgcg tggcaagttg
      481 aattcaaacg aagcaatgtg aatgctaaat tgagcggcat tgataaagtt aatcaatttt
      541 tcttctatag acccggcggg gttattgagg tcattaatcg tcaatggttc ggtgaaatac
      601 acttcccacc attttgcaac ggggaaagga gtatcggtta caaagacagg ttgttcaaga
      661 gtaggcgtct ctgtgacggg aaaaatgatc tgcggagtct cagttaccgg aacaataaaa
      721 ggcgcaggcg tatcactggc ataaaaagga atctgtgtga ccggtgtata tggggtttca
      781 atatcgagag gagcacaagc ggtaaggaag ataaaaatca ggagagcagc tagtttcttg
      841 ttcattatgg gttcctgaat caaaagtgac agtcgctgta ttgtaccgga aagcgactgt
      901 caccctgagt gacttaaccg taaaaaactt attctaatgc tgaaaaatct tgcggattcc
      961 ctaacggata gctgactgca tcactcacaa tacgcttgac caaaccaccc aatacagagc
     1021 ctgtccctac ctcaacaaaa gcggtcacgc ccatgttact gatggttttt acactatccg
     1081 tccatttcac gcgagattgc atctgcgctt tgatatcggc tcgagcttct tctgctgttt
     1141 taagcacaga agcgtggacg ttcccaatca ctggcgtctt cgggtggtgc atactggctt
     1201 catctacagc ctgattccat tcatcttgaa tagacgccat caacggggag tgtgccgcaa
     1261 ttgacactgc caatggcaag gctcgttttg caccagcagc tttcgcacct gccatggcgc
     1321 gctccactgc tgctttggag ccagagatca ccacttgccc agggcaatta tcatttgcaa
     1381 cttgtaccaa ctcatctgtg acactggctt cggcacaaac tttatcaagt gtgggaatgt
     1441 ctacaccgag gatggcagcc atgctaccgg gagccagttc acctgcacgc ttcatcaact
     1501 caccgcgttt gcgcacgagg cgtaacccgt cttcaaaaga taaggctcca gaggcggtca
     1561 gagccgataa ctcgccaagt gaatgcccgg caagtgcagc aggcacgagg tccgggtaga
     1621 ggcgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc aggttgcgtg ttgaccgtat
     1681 catttaattc gacgtctggc ccattccaca tcaggtctga gagcgcaaag ccgaggatcg
     1741 aatccgcttc ggcaaagact tctttggcta tcgtttgttc ttcggcaagg gattttccca
     1801 tccctaccgt ttgagagcct tgtccgggga agacaaaggc tgtgttgttc aaatttaatt
     1861 tcattccgtc tccttgtggc taaaaatcaa tatatttcag ctatttacag cattgttagg
     1921 catggctatt aaacacaaaa ttgcaaatat ggtcacgca
//
LOCUS       DAOQNJ010000528         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_50594_length_1951_cov_9.318038, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000528 DAOQNJ010000000
VERSION     DAOQNJ010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50594_length_1951_cov_9.318038"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLG52_12545"
     CDS             <1..620
                     /locus_tag="PLG52_12545"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNB42325.1"
                     /translation="NVTRPTHRNTSWDWARFETCAQKWVDLSEGDYGVSLLNDCKYGH
                     DIHDNVIRITLLRSPTMPDPMADFGEHEFKYSLYPHVGSWNEETQREAYLLNDPIISY
                     RSRVEGRKSGKPISNLQSLLSCASPNIIIETVKQAENGDGIIVRLYESQRKRGQVRVR
                     FRSDVESAWVTNLLEENESALSVDQASIILNLKPYQIMTLRVKLK"
     gene            617..1108
                     /locus_tag="PLG52_12550"
     CDS             617..1108
                     /locus_tag="PLG52_12550"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HNB42326.1"
                     /translation="MMNEQDFLAFMDANGFVYERIEHPAVFTCAESELHRPAVPAVST
                     KNLFLCDKKARRFFLAVTACEKTVKLDALAGQLGVANVRFASEENLMRLLGVTRGSVT
                     MMGLANDTEHIVELWVDAEIWQSDYFLSHPLVNTATLILKKPELERFFTLTGHELHLF
                     AGE"
     gene            1110..>1951
                     /locus_tag="PLG52_12555"
     CDS             1110..>1951
                     /locus_tag="PLG52_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB42327.1"
                     /translation="MRIAFAGTGYINKVHAQAAQNLGLELAAVVNHKADSMAEFGKRF
                     GITRQYESVDAMLKDGDVDALVVSTPNYLHAPQTIFALNAGVHVMVEKPMAMNAAEAE
                     QMNSAAEKSGATLMVAHCWRFDPDVLWLKEQSAKLGKIIRTKGYGVHTHWGPSGWFTQ
                     KQFAGGGAMADMGIHALDTARFLLGDPQPVSVYAKIGTYYKDFDVDDTGVIIVEWDNG
                     ATSYIESGWWQVHSDGPEAATQLYGLEGFGQLFPTRVEIRMMKEERIDVVESGFKFPR
                     DEHCP"
BASE COUNT          486 a          434 c          575 g          456 t
ORIGIN      
        1 gcaatgtcac ccgtccaacc caccgcaaca ccagttggga ttgggcgcgc ttcgagacct
       61 gtgctcaaaa atgggtggat ctctccgaag gcgattacgg tgtgagtctg ctcaacgatt
      121 gtaagtacgg gcatgatatc catgacaatg tgattcgcat caccctgctg cgctcgccga
      181 ccatgcccga cccgatggcg gattttggtg agcacgaatt caaatacagt ttgtatccgc
      241 acgtgggttc atggaatgaa gagacacagc gcgaagcgta tttgttgaat gacccaatta
      301 tcagttacag gtcaagggtt gaaggtcgca agtctgggaa accaatatct aatctccaat
      361 ctctactctc ttgcgcttcg cccaacatca tcatcgaaac ggtgaagcaa gcggaaaatg
      421 gcgacgggat catcgtccgc ttgtatgaaa gccagaggaa gcgggggcag gttcgggtga
      481 gattccgaag cgacgttgag tctgcctggg tgacgaatct actagaggag aacgaatccg
      541 ctttgagcgt ggatcaggct tcgatcatcc tgaatttgaa accatatcag atcatgactt
      601 tgcgggtaaa actcaaatga tgaacgaaca ggactttttg gctttcatgg atgcgaatgg
      661 atttgtgtac gagcgtatcg agcaccctgc cgtgttcacc tgcgccgagt cggaattgca
      721 tcgtcctgca gtgcctgcgg tgagcacgaa aaatttattc ctgtgcgata aaaaggcgcg
      781 ccgctttttt ctggcagtga ccgcctgtga aaagaccgtg aaactggacg cgctggcagg
      841 tcagttagga gttgcgaatg tgcgctttgc atcggaagaa aacctgatgc gcctgcttgg
      901 cgtgacccgc ggctcggtga ccatgatggg gctggcgaac gatacggaac atatcgtgga
      961 gttatgggtc gatgcagaga tctggcagag cgattatttt ctgagccatc ccttggtgaa
     1021 tacggcgacc ttgattttga agaagcccga actggaacgc tttttcacgt tgactgggca
     1081 cgagctacat ttgtttgcag gagaataaga tgagaattgc atttgcaggc acgggctata
     1141 tcaacaaagt ccacgcgcag gcggcgcaga atttgggcct ggagttggcg gcggttgtaa
     1201 accacaaagc ggactcaatg gctgagttcg gcaaacgctt tggcatcaca cgccaatatg
     1261 aaagtgtgga tgccatgctc aaagatgggg atgtggatgc gctggtggtt tccacaccca
     1321 attatctgca tgcgccgcag accatttttg ctttgaacgc aggcgtgcat gtgatggttg
     1381 agaaaccgat ggcgatgaac gccgccgaag cggagcaaat gaattcggct gcagaaaaat
     1441 ctggcgccac attgatggtg gcgcactgct ggcgctttga ccccgatgta ttgtggctga
     1501 aagagcagtc ggcaaaatta ggaaagatca ttcgcacgaa aggctatggc gtgcatacac
     1561 attgggggcc gagtggttgg ttcacacaaa aacaattcgc gggcggcggc gcgatggcag
     1621 atatgggcat ccacgccctg gatacggctc gctttttact cggcgacccg cagcctgttt
     1681 cggtgtatgc aaagatcggt acctattaca aagacttcga cgtggatgat acgggtgtca
     1741 ttatcgtcga atgggataac ggcgcaacat catatattga gtcgggttgg tggcaggtgc
     1801 atagtgacgg ccctgaagcg gcgacgcagt tgtatggtct ggaagggttt ggtcaattgt
     1861 ttccgacaag agtggagata agaatgatga aagaggagag aattgatgtg gtcgaatctg
     1921 gtttcaagtt tccaagagat gaacattgtc c
//
LOCUS       DAOQNJ010000529         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_50596_length_1951_cov_7.959388, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000529 DAOQNJ010000000
VERSION     DAOQNJ010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50596_length_1951_cov_7.959388"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..>1951
                     /gene="glmS"
                     /locus_tag="PLG52_12560"
     CDS             217..>1951
                     /gene="glmS"
                     /locus_tag="PLG52_12560"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNB42328.1"
                     /translation="MCGIVGYIGPREATPIILNGLKRLEYRGYDSAGVAVINNNQIDM
                     RRDAGKLQQLVDLVAKSPLVGAPGIGHTRWATHGAPSARNAHPHIGNTGKMVVVHNGI
                     VENFLELKDELTAEGVTFNSDTDTETIVHLAEHHKAAGISFEEAARRTFKQIEGANVV
                     LLLSTDEPDKIIAARIGNAGGVVIGLGDGENFLASDIPAILEHTRRMIFLESRQMVVV
                     TKDSVRIETLDGEAVQPEIHTIAFDAVSAEKGEYRHFMQKEIHEQVRALTDTLAGRVD
                     FKEGRIRLPELNLTPELAKRIQRIYITACGTAAYAGMVGKYLIEKIARVPVEVVIGSE
                     FRYSDPIVDENTVVLAISQSGETADTLAAMEEGRKKGGIVWSIVNAIGSQAMRIADGY
                     ISMQTGPEIGVASTKAFTAPLVDQYMLAILLADLRGVIDDKTRRELVADLRLVPDLAG
                     RVMDTEADIEKVAHALKDLKGCLYLGRGINMPIAYEGALKLKEISYIHAEGYPAGEMK
                     HGPIALIDEEMPVVCITPKDPWHEKMISQIQQAKARGGMVIAVATEGDELVKGMADHV
                     LWIPDAPWMLSP"
BASE COUNT          504 a          456 c          535 g          456 t
ORIGIN      
        1 tggaaaatcg agagatcagc ggaagccttc cgggtctgcc atgacccgcc catctttccc
       61 tccagagatg aaacccaggt tacaaccccg tgagcgatcg cggatcaaaa ggcttgggcg
      121 tggcaaagat atcgggctgt aagagcaggc gtcgctttgt tgtgaggcgc ctggttttca
      181 gttctcaatt caatcaaaat ttggaggttt cctactatgt gtggaatcgt cggttatatc
      241 ggtccgcgtg aagcgacacc gataatcctt aacggcttaa agcgccttga atatcgcgga
      301 tatgactctg cgggcgtggc tgtcatcaat aacaatcaga ttgatatgcg cagagatgcg
      361 ggcaagctgc aacagttggt ggatttggtt gccaaatcgc cgctggtggg tgcgccaggc
      421 attgggcata cacgttgggc aacgcacggg gcgccatcgg ctcgtaacgc tcacccccac
      481 attggcaata ccggcaagat ggtggtggta cacaatggga tcgttgaaaa ctttttggaa
      541 ctcaaagatg aactgaccgc tgaaggcgtc acgttcaatt cagataccga tacagaaacc
      601 atcgtgcatt tggcagagca ccacaaagct gccggaattt cttttgaaga agctgcccgc
      661 cgcactttca aacagatcga aggggcgaat gtggttttgc ttctttccac agatgaacca
      721 gataagatta ttgcggctcg cattgggaat gccggtggtg tagtaattgg gttgggcgac
      781 ggggagaatt tcctcgcttc agacattcct gctattttgg aacacacccg cagaatgatc
      841 ttcctcgaat cgcgtcagat ggtggttgtg accaaggata gcgtccgcat agaaacatta
      901 gacggtgaag cggttcagcc ggaaattcat acaatcgcat tcgacgctgt ttctgcagag
      961 aaaggcgaat atcgccactt tatgcagaaa gaaattcacg agcaagtgcg cgcgctgacc
     1021 gatacgcttg ctgggcgtgt ggatttcaaa gaaggtcgca ttcgtttgcc tgagttgaac
     1081 ctaacgccag aacttgcaaa acgtattcaa cgtatttaca tcacggcttg tggaacagct
     1141 gcttatgcag gcatggttgg taaatacctc attgaaaaga tcgcacgcgt tccagtggaa
     1201 gtggtcatcg gttctgagtt tcgttacagc gacccaatcg tggatgaaaa cacggtagtc
     1261 cttgcgatct cacaatcagg cgaaacggct gacacattgg cagccatgga agaaggacgc
     1321 aaaaaaggcg gcattgtttg gagtatcgtc aatgccattg ggtcacaagc catgcgtatt
     1381 gcggatggat atatctcgat gcaaaccgga cccgaaattg gtgtggcgtc taccaaagcg
     1441 ttcaccgcac cgctcgtgga tcagtacatg ctcgccatcc ttctggcaga cttgcgcggt
     1501 gtgatcgatg ataaaacccg ccgcgagctg gttgcagact tacgcctcgt gccagatctg
     1561 gcaggcagag taatggacac agaagccgat atcgaaaaag tagcccacgc gctcaaagac
     1621 cttaagggtt gtttatattt gggtcgtggc atcaatatgc cgattgcgta tgaaggtgct
     1681 ttgaaattga aggaaatttc ctacatccat gcagagggct acccagccgg tgagatgaag
     1741 catggtccta ttgcgttgat cgatgaagaa atgcctgtag tctgtattac gcccaaagac
     1801 ccttggcacg agaaaatgat ctcacaaatt cagcaagcaa aagcacgtgg cggcatggtg
     1861 attgccgttg ccaccgaagg cgatgaattg gtgaaaggca tggcagacca tgtgctttgg
     1921 atcccagatg caccgtggat gctctcaccg a
//
LOCUS       DAOQNJ010000530         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_50774_length_1947_cov_7.974101, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000530 DAOQNJ010000000
VERSION     DAOQNJ010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50774_length_1947_cov_7.974101"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..799)
                     /locus_tag="PLG52_12565"
     CDS             complement(257..799)
                     /locus_tag="PLG52_12565"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNB42329.1"
                     /translation="MQTFLKFSHAIDSFTDRLGQASAFCVMLTIAVGFYNVIARYLGR
                     LIEVKLTSNLFIELQWYLFSLTFLLGFSYVLKHGLNVRVDFLYTNWSAKTKAWVDLVG
                     HAIFLTLFCIIGLYVTTHPVLQSWGLLPNGSFGTWELSPDPDGLPRAPIKSMMLVAFG
                     MLFLQTISEIIKNIAKIKEH"
     gene            883..1410
                     /locus_tag="PLG52_12570"
     CDS             883..1410
                     /locus_tag="PLG52_12570"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNB42330.1"
                     /translation="MAKNIVLTLTGKDKIGLVDQVTSLVVKRNGNVESSRMARLGGEF
                     AMLMLVTVSDAEFQNLDKDFQSLRGEGYQVTLMQTEDDSKKYAGWLPYEIEVTGADHE
                     GIIQQISHHLASQGINIESMDTSSAPAPMSGTPLFTMKGIVLVPAKLNFNTWSDALEE
                     VGDKLNVSVKVEVVK"
     gene            complement(1517..>1947)
                     /locus_tag="PLG52_12575"
     CDS             complement(1517..>1947)
                     /locus_tag="PLG52_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB42331.1"
                     /translation="GQVVMALIQMSLLILFGVFALKLKWGQEPFALFVLLTCAALAAG
                     SIGTMMGTFIKTEGQANGLSIMAGMVMALLGGCWYPLELFPAVVQNIVKILPTTWAMQ
                     GLLDLVLRGGGLVDILPESAVLLGFAGVFFTIGVMRFRYE"
BASE COUNT          565 a          434 c          501 g          447 t
ORIGIN      
        1 gactcggcat gagcttaagc agattgaggt tgaatgcgcc aagagcgtag ccaataccgc
       61 cgaagagaat cgcagtggcg gcgaaggaat agccgacggg gtatccgctc atgatgaagg
      121 cgaagaagcc gacgaacata aagatggcga ggaattcgta gtgagacata aaccctttaa
      181 ttctttaacc accaaggtca caaagtacac aaaggggatg cttttgaaac cttcgtgacc
      241 gttgtgtcct ttgtggttaa tgctctttga tttttgcgat gtttttgatg atctcagaaa
      301 tggtttgcaa gaacaacatt ccaaatgcaa caagcatcat agacttgatc ggggcgcgcg
      361 gtagaccgtc tggatcgggc gagagttccc aggtgccgaa ggagccattg ggcagcaagc
      421 cccaagattg taagacggga tgtgtggtga cgtataagcc gatgatacaa aagagggtca
      481 ggaagattgc atgacccact aagtctaccc aggcttttgt tttggcagac caatttgtat
      541 atagaaaatc cacacgcaca ttgagcccgt gtttgaggac gtaggagaac ccgagcagaa
      601 aggtgagcga gaagagatac cactgtaatt caatgaagag gttactggtg agttttactt
      661 cgataagcct gccgagataa cgcgcgatca cgttataaaa tccgaccgcg atggtaagca
      721 tcacacaaaa tgcagaggct tgaccaagcc ggtctgtaaa tgagtctatt gcatgggaga
      781 atttgagaaa ggtctgcatg gactggcgga tgatagcata aaaccaaaga tgcttgtgct
      841 atactgggta ctatatttca caagtcgaaa gggagtgctg ccatggcaaa aaatattgta
      901 ttaactctca caggcaaaga taagatcggg ttggtggatc aagtaacaag tctggttgtg
      961 aagcgcaacg ggaatgtcga atccagccgc atggcgcgtt tgggcggcga gtttgccatg
     1021 ctgatgttgg tgaccgtctc cgatgcagaa tttcagaacc tcgataaaga ttttcaaagt
     1081 ttgcgcggcg agggctatca agtcaccttg atgcagaccg aagatgactc gaaaaagtat
     1141 gcaggctggc tgccatatga gatcgaagtg accggcgcag accatgaagg catcattcaa
     1201 cagatttcac atcatctggc ttcgcagggc atcaatatcg aaagcatgga tacgagttcc
     1261 gcgcctgccc caatgagtgg gactccgctc tttacgatga agggcattgt gcttgtgcca
     1321 gcaaagttaa acttcaatac ctggagtgat gcactggaag aagtggggga taaattgaat
     1381 gtgagcgtga aggtagaggt tgtgaagtag tttcaagcaa ccagtaaata gtgatcagta
     1441 accagaaggc ggaatgcagt taatctgctt ccgccttttt cttttttctt tctcgttact
     1501 tgttactcac ttctcatcac tcgtaccgaa atctcatcac cccaatcgta aagaacactc
     1561 cagcaaagcc aagtaacact gctgactcag gcaaaatatc aaccaaaccg ccaccacgca
     1621 gaacaaggtc gagcaagcct tgcattgccc atgtagttgg cagaattttt acaatgtttt
     1681 gcaccaccgc ggggaaaagt tccaacggat accaacaacc accgagtaat gccatcacca
     1741 tgcctgccat aatgctgagc ccattggctt gtccttcggt cttgatgaat gttcccatca
     1801 tagtgccgat cgaacccgct gccagagcgg cgcaggttaa taggacaaag agagcgaacg
     1861 gctcttgccc ccatttgagt ttgagtgcaa agaccccaaa cagaatcaac agggacattt
     1921 ggatcaacgc catcacgacc tgacccg
//
LOCUS       DAOQNJ010000531         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_51128_length_1940_cov_9.558621, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000531 DAOQNJ010000000
VERSION     DAOQNJ010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51128_length_1940_cov_9.558621"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..274)
                     /gene="rpsT"
                     /locus_tag="PLG52_12580"
     CDS             complement(2..274)
                     /gene="rpsT"
                     /locus_tag="PLG52_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNB42332.1"
                     /translation="MANIQSQIKRNRQNEKRRLRNRNVRGAARTADNAARKVLTSDTP
                     ESKQAVMQAISALDKAAEKGVLHKNTVARRKSRLMKALAALTKKSA"
     gene            complement(394..1206)
                     /locus_tag="PLG52_12585"
     CDS             complement(394..1206)
                     /locus_tag="PLG52_12585"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB42333.1"
                     /translation="MTLRTPSTPPSGTIGSYRRRNRGRQNAIVIVAGVLLLGGLALLI
                     YWMTLPGKPINLLLATETPTPTLTYTPTNTNTPTPTFTETPSPTITFTPTFSAPFTYT
                     VQDGDSLAVIAERFNLGEDAIPLILLLNPYVVPTDPAQFPQGVDPTTLNVIPGQVILL
                     PNPDMQLPTATPIPPNLPRGTKIDYVIQSGDSLGAIANAFNSTIDDIMKENNITDPNA
                     LFVGQVLVIPVNMVTPTATRPPTSTPITPGPGTVLPTGTSTPINAAPATATP"
     gene            complement(1350..>1940)
                     /locus_tag="PLG52_12590"
     CDS             complement(1350..>1940)
                     /locus_tag="PLG52_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNB42334.1"
                     /translation="ELRIEMQFLPDVYVPCDVCHGKRFNRETLQVMFRDTYSIADVLD
                     MTVDHALEEFKNYPQMQNKLKLLQEVGLGYVRVGQPATTLSGGEAQRVKLSKELSRRA
                     TGRTLYVLDEPSVGLHAADVHKLIEVMQRLVDTGNSVLIIEHNLDIIKVADWLIDLGP
                     EGGNRGGELIAEGTPEQVMKVKGSYTGKYLKDHINR"
BASE COUNT          451 a          439 c          523 g          527 t
ORIGIN      
        1 attacgcgct ctttttggtc aacgctgcaa gggctttcat caagcggctc ttgcgacgcg
       61 cgacggtgtt cttgtgcagc acaccttttt cagcagcctt atccaaagcg ctaatggctt
      121 gcatcacagc ctgcttggat tcgggggtgt cgctggtgag aactttgcgc gcagcgttat
      181 cggcagtacg ggcggcgcca cggacattgc ggttacgcag gcgacgcttc tcgttctgac
      241 ggtttcgttt gatctgagat tgaatgttag ccaaggtctt cctccaaaaa attcttgcct
      301 tactttacga cggcgaccta ttttacatgg cacaggcata tttgtccagt aaaatgataa
      361 gaaaaccgcg ggcaatttgc ccacggtttt tgtttatggg gtggctgttg ccggcgccgc
      421 attaatgggg gtggaggtgc cggttggcaa aacggttcct ggtcccggtg tgatgggggt
      481 gcttgtgggt gggcgggtgg cggtaggtgt caccatgttt acaggaatta ccaaaacctg
      541 tccaacaaaa agggcattgg ggtctgtaat attattttcc ttcataatat cgtcaatagt
      601 gctattgaag gcattggcaa tggcacctaa agagtcacca gattggatga cgtaatctat
      661 tttcgtgccg cgcggcaagt taggtggaat gggtgtggct gtaggcaatt gcatatctgg
      721 gtttggaagc aaaatcacct gcccaggaat cacgtttagg gtagttgggt caacgccttg
      781 tgggaattga gccggatcgg ttggaacaac atacggattc agcaaaagaa tcagcggaat
      841 ggcatcctcg ccaagattga atctttctgc gatcactgcc agagagtctc catcttgtac
      901 tgtatatgta aatggagctg agaaggtcgg tgtgaaggta atggtgggcg aaggagtttc
      961 tgtgaatgtt ggtgtaggtg tatttgtatt tgttggcgta taggtgagtg ttggggtagg
     1021 ggtttcagtt gccaacaaca ggttgatggg tttgccgggg agagtcatcc aatagatcag
     1081 caatgcgaga cctcccaaca ggagaacgcc ggccacaata acgatggcgt tctgcctgcc
     1141 gcggtttcgt ctgcggtagg agccaatggt tccagaagga ggggtgcttg gagttctgag
     1201 agtcatttaa tcaatccttt tgcacgtcgg tctaaatgta ttttccaacc aattgcagtg
     1261 caaaataatt ctacctgaaa aaggggaatc gcgtgtgcat ggcacaaaaa aacaggctga
     1321 cattgccagc ctgttttcaa aatgctctat tagcggttta tatggtcttt caagtatttg
     1381 cccgtataag agcctttcac cttcataact tgttctggtg tgccttcagc gatcaactcg
     1441 ccgccacggt taccgccttc tggaccaagg tcaatgagcc aatctgctac tttgataata
     1501 tccagattgt gctcgatgat aagcactgag ttgcccgtgt caacgagccg ctgcatcact
     1561 tcgatcaact tgtgaacgtc tgctgcatgt aaaccgacag aaggttcatc caatacgtaa
     1621 agtgtacgtc ccgttgcccg gcgagaaagt tctttcgaga gtttcacgcg ctgtgcttca
     1681 ccccccgaga gcgtcgttgc gggttgaccc acccgcacat atcccaaccc tacttcctgc
     1741 aagagcttca acttgttctg catttgtggg tagttcttga attcttccag tgcatggtct
     1801 accgtcatat cgagtacatc agcaatcgaa tacgtatcac ggaacatcac ctgcaaggtc
     1861 tcgcggttga agcgtttgcc gtgacacaca tcgcaaggca catacacatc tggcaaaaat
     1921 tgcatttcga tgcgtaactc
//
LOCUS       DAOQNJ010000532         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_51165_length_1939_cov_15.377919, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000532 DAOQNJ010000000
VERSION     DAOQNJ010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51165_length_1939_cov_15.377919"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1157
                     /locus_tag="PLG52_12595"
     CDS             135..1157
                     /locus_tag="PLG52_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB42335.1"
                     /translation="MKRFGIIGCGRIAPRHADSLAEIEGTKLVQVCDVIESRARHFAE
                     KYDTEYCLDYHELLANRDIDIVNICTPSGLHAEMAIAALRAGKHVIVEKPMAMNVADA
                     DRMIAAAKEMNRKLCIVLQNRYNPPMQDFYKAVRDGKIGRVLLGSVTVRWYRPQEYYN
                     DGWHGTKSMDGGALMNQSIHHIDALQWLVGVPVKSVFAYTATLAHQMEAEDVGVVTVR
                     FENGALATIEGSTVTYPQNLEASVALFGEKGSLKVGGTALNRKVFWKIAGEIEHEKEL
                     LTREEVDPPSVYGASHRHVLEDMISAVEDDREPQTNGVEGRKSLALVSAMYESARTSR
                     EVILAA"
     gene            1168..1782
                     /locus_tag="PLG52_12600"
     CDS             1168..1782
                     /locus_tag="PLG52_12600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNB42336.1"
                     /translation="MMQNSETSIIFPNVVWEGEYQLGHFCVIAQPFKGGEAAETRIGV
                     NATIRSHSVIYAGNVIGKNFQTGHGVMIRELNQIGDNVSIGTHSIVEHHVVIGNGVRV
                     HSNVFIPEYCVLEDECWIGPAVVMTNAVYPRSKSVKEKLTGAIIEKGAKIGAGAVLLP
                     GVRIGRNALIGSGAVVTRDVEENGVVVGNPSRLVNHITNIPDYQ"
BASE COUNT          512 a          393 c          551 g          483 t
ORIGIN      
        1 ggcgacgatc agatgatgga cattctacgc ctcacctgcc tcgaagccgg gctaaagatc
       61 agcgacctgc ccgaagattg cgtactcgag tcaaatgggc acgggtatcg tgtgcggaca
      121 aaaggattaa gttaatgaaa cgattcggga tcatcggctg tggacggatc gcgccgcgcc
      181 atgctgactc tttggctgaa attgagggaa caaaactggt tcaagtttgt gatgtgatcg
      241 agtcacgcgc ccgccatttt gcagagaagt atgatacaga atactgtctt gattatcatg
      301 agctgcttgc caatcgagat attgatattg tcaacatctg cacaccgagc gggttacatg
      361 ctgaaatggc aattgcagca ttaagggctg gcaagcatgt catcgtggaa aagccaatgg
      421 cgatgaatgt tgccgatgca gatcggatga tcgcagcggc gaaggaaatg aatcgcaagt
      481 tgtgcattgt tttacagaat cgctataacc cgcccatgca ggatttttac aaagcagtgc
      541 gagatggcaa gatcgggcgc gtccttcttg gatcggtaac ggttcgctgg taccgcccac
      601 aggaatacta caacgacggc tggcacggaa ccaaatccat ggatggcggc gcgttgatga
      661 atcaaagcat tcatcatatc gatgccttgc agtggttggt gggtgttccg gtaaagagtg
      721 tctttgcgta cacggcaacc ctggctcatc aaatggaggc ggaagatgtg ggcgtggtaa
      781 cggtgcgatt tgaaaatggc gcattggcta ccattgaagg ctcgaccgtg acctatccac
      841 aaaatcttga agcctcagtg gcattgtttg gcgaaaaagg ttcactaaaa gtgggcggca
      901 cggcattaaa tcgcaaagtg ttctggaaga tcgcaggcga gatcgaacat gaaaaggaat
      961 tattgactcg tgaagaggtt gacccacctt ctgtctatgg tgccagtcat cggcatgtgt
     1021 tggaagatat gatctccgca gtagaggacg accgtgagcc gcagaccaac ggggtggaag
     1081 ggcgcaagtc tcttgcgttg gttagtgcga tgtatgaatc agctagaact tcgcgagaag
     1141 taattcttgc cgcatagtga gtgacctatg atgcaaaatt ctgaaacttc aattattttc
     1201 ccaaatgtgg tttgggaagg ggaatatcaa ctgggtcatt tttgtgtgat cgctcagcca
     1261 ttcaaaggcg gtgaagccgc cgaaacacgc attggagtca atgcaactat tcgctcccat
     1321 tctgtcattt atgccgggaa tgtgatcggt aaaaacttcc aaacgggtca tggtgtgatg
     1381 atccgtgagt tgaatcaaat cggagacaac gtcagcattg gcacacactc cattgtggag
     1441 catcacgttg ttattggaaa tggtgtgcgc gtccattcga acgtttttat tccagagtat
     1501 tgtgttcttg aagatgaatg ctggatcggt ccggctgtgg tgatgacaaa tgccgtgtac
     1561 ccgcgatcca aaagtgtaaa ggaaaaattg acaggcgcaa ttattgaaaa aggcgcgaag
     1621 atcggtgcag gcgcagtgtt attgcccggt gtgagaatcg gtcgtaatgc gctgatcggt
     1681 tccggcgcgg tggtgacgcg tgacgtcgaa gaaaatggtg tggtggttgg gaatccatct
     1741 cgattggtca atcacattac gaatattccg gattatcagt aggagaattc atgactgaaa
     1801 aaattccttt gttagatctg cgtgctgaat atgccgagat caaagggtct gtggatgcag
     1861 ccatccaacg agttgtggaa agcgcacgtt ttattttagg ggaagaggta acaaattttg
     1921 aaaaggattt tgctgcctt
//
LOCUS       DAOQNJ010000533         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_51212_length_1938_cov_9.631439, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000533 DAOQNJ010000000
VERSION     DAOQNJ010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51212_length_1938_cov_9.631439"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLG52_12605"
     CDS             complement(<1..356)
                     /locus_tag="PLG52_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB42337.1"
                     /translation="MFEILFLGTSASAPSAHRGLSSHIVSHNEYRFLVDCGEGTQRQI
                     LQSGKGFKRLTRALITHGHLDHILGLGGLLSTFLRWEAIEEFEVYGSKGTLERVKTLL
                     FDVVLRGKKTPMPLRL"
     gene            632..>1938
                     /locus_tag="PLG52_12610"
     CDS             632..>1938
                     /locus_tag="PLG52_12610"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNB42338.1"
                     /translation="MPLLKPSAPQSNSKKPAGKTLPAKKGGADLPSKAPAKGRGLPPQ
                     APPSPPPSYTPQPLREQTWWEKLSPERKLDVVGVILAFFGLVILLGLLSANRSAIVGG
                     AIFFLAQLFGWGVYVLPIGLLVFGLWLVFRKIERIPPLSTERAVGSVILFFWLMTLLH
                     SFIATAETAEAVALTGQGGGYFGGLAQRILWGAFGAGGAFIAMIAWLIIGVAVLLDKP
                     VIELFFWLKPLMLKLRDLIIKPAPQTQPVTAQPQSSDGLTPIDSTAMPAMDMTNAVPV
                     QPVQTRSGTTIIEWKLPDLTDILDEGSEATVNEDFIKQRAHLIEETLASFGAPAQVVA
                     ISRGPSITQFGVEPLFLESRGGVRTRVRVSKIASLADDLALALAAPRIRIQAPVPGHS
                     YVGIEVPNDEMARVALRDILEGEIFKGYKKPLAFALGRDVAGLP"
BASE COUNT          500 a          533 c          439 g          466 t
ORIGIN      
        1 atcaaacgca aaggcatggg cgttttcttg ccgcgcaaca ccacatcgaa caacaaagtc
       61 tttacacgtt caagcgttcc cttactgcca tacacttcaa actcttcaat cgcttcccaa
      121 cgcaaaaaag tggaaagcaa cccacccaac cccagaatat gatccagatg cccgtgtgtg
      181 atgagcgcac gcgttaaccg cttaaagccc ttgccagact gtaatatctg gcgctgggtg
      241 ccttcgccac aatcgaccaa aaaacgatat tcgttatgag agacgatatg agaagaaagc
      301 ccgcggtgtg ccgatggcgc agatgcggat gtaccaagaa atagaatttc gaacaaaatt
      361 gtgtcctatc aaactgcgga ggaaatgtag ccccgcgaat caatcgctga aaaatactct
      421 tatcgcacac aaccaaactg gcaacaagcc ctgcaatttt accttaactc aaatcaacta
      481 ttgggcaaaa acccatttaa tgaagaatac gtttgaaatg aaactcaata gaatcagcac
      541 aagaagttaa acagaccatt tgtgctataa tgactcaaga acgatttttt atcgtaaatg
      601 atgaacgaac ctatttcctc ttgaaaatcg aatgcctctc ctaaaaccct ccgcgcctca
      661 atcgaatagt aaaaagcccg caggcaaaac tcttcccgca aaaaaaggcg gcgcggattt
      721 accttccaaa gctccggcaa aaggcagagg gcttcctcct caagctccac catccccacc
      781 tccatcatat acaccccaac ccctgcgtga acaaacctgg tgggaaaaac tttcacccga
      841 acgcaagtta gatgttgtag gtgtcatcct cgcatttttt ggtctcgtca ttttattagg
      901 gcttctttct gccaaccgct ccgctatcgt tggtggcgcg atctttttcc ttgcacaact
      961 atttggctgg ggcgtatacg tcttacccat tggcttgctc gtctttggat tgtggctggt
     1021 cttccgtaag atcgaacgta tccctccgct ttctacagaa cgcgcagttg ggagcgtcat
     1081 ccttttcttc tggctgatga ccctgttgca ctccttcatc gccacagctg aaactgccga
     1141 agcggttgca ctcactggtc aaggtggcgg gtatttcggc ggtttggctc aacgcattct
     1201 ttggggagcc tttggcgcag gcggtgcctt catcgccatg atcgcctggc tcataattgg
     1261 cgtcgccgtt ttgctagaca aacccgtcat cgaacttttc ttctggctca aaccgttgat
     1321 gctgaaactg cgcgacctga ttatcaaacc cgcgcctcag actcaaccgg tcactgctca
     1381 gccccaatca agtgatggac tcacgccgat agattctacc gccatgcccg ccatggacat
     1441 gacgaatgcc gtccccgtac agcccgttca aactcgcagt ggcacaacca tcatcgaatg
     1501 gaaattgcca gacctgacag atattcttga cgaaggcagt gaagcaaccg tcaacgaaga
     1561 tttcattaag caacgcgcac atctcattga agagacgctc gcttcctttg gcgcgccagc
     1621 gcaagtagtg gcaatcagcc gtggaccttc gatcacgcaa tttggggtgg agccgctatt
     1681 tcttgagtcc cgaggcggcg tgcggaccag agtccgtgta agtaagatcg catcccttgc
     1741 tgatgatctc gcccttgcac tagccgcgcc gcgcatccgt attcaagcac cggtaccggg
     1801 gcatagttat gttggcatcg aagtgccgaa tgacgaaatg gcgcgtgtag cgttgcgcga
     1861 tattttagaa ggtgaaattt tcaaagggta taaaaaacct ttggcatttg cgcttgggcg
     1921 tgacgtggct gggttgcc
//
LOCUS       DAOQNJ010000534         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_51461_length_1933_cov_9.215655, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000534 DAOQNJ010000000
VERSION     DAOQNJ010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51461_length_1933_cov_9.215655"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /gene="secD"
                     /locus_tag="PLG52_12615"
     CDS             <1..936
                     /gene="secD"
                     /locus_tag="PLG52_12615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNB42339.1"
                     /translation="TDFAAGDTTAAATPAPDGTTPVIYHTVMTGTSLDTASVVRSQTG
                     EYEISFTLKADAASVFGEYTTTHTNQYLAIVLDKVVISTPVIRQPITSGQGVISGNFT
                     SESAQTLAIQLRSGALPIPIKVVESRTVGPTLGEESVRKSVLAGLIGLTVVVLFMGFY
                     YRLPGVIADLALICYAIFSLMLFKLIPVVLTLPGIAGFILSVGMAVDANVLIFERLKE
                     ELRAGRNLRHAIDLAWSRAWSSIRDSNTSTLITCVILFIFGSTFGASMVKGFAVTLAI
                     GVLVSLFTAITVTRTFLHAILDNVKNTEHPSWFGL"
     gene            946..1890
                     /gene="secF"
                     /locus_tag="PLG52_12620"
     CDS             946..1890
                     /gene="secF"
                     /locus_tag="PLG52_12620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNB42340.1"
                     /translation="MNIIKNRYLYFLISLLVIIPGLVFMGLNWANTKQGPLVLGIDFR
                     GGSLLEIQFEGTRPSSSDIAALYAEFSTEEENFKDPVIQPLGEDAYSIRSKTMTDETK
                     GKLVAEMESRFGSKITVLNFTSVSASVGAEVTRAAGVAIFMAAAAILIYIWWAFRSVE
                     HAYRYGTAAIIAMLHDVFVVLGAEAILGYFFGWEADALFLTALLTVIGFSVHDTIVVF
                     DRVRENSNLGRKIGYESMINHSIVQTLDRSLTTQLTVMFTLFALSLFGGDSIRHFTII
                     LLVGILSGTYSSIFNAAPILVVWENREWTTWFGRKASA"
BASE COUNT          418 a          505 c          445 g          565 t
ORIGIN      
        1 accgacttcg cagcaggcga tacaacggct gccgccaccc ctgcccccga tggcacgaca
       61 cctgtgatct atcacaccgt gatgaccggt accagcttgg atacagcaag tgttgtccgc
      121 agccagaccg gtgaatatga aatttcattc acgttgaagg ctgatgccgc tagtgtcttt
      181 ggtgaataca ccacaaccca tacaaatcaa tatcttgcaa tcgtgctgga taaggttgtg
      241 atttctaccc ctgtaattcg ccaacccatc acaagcggac agggcgttat ctctgggaac
      301 ttcacttccg aatctgccca gaccctcgcc attcagcttc gttctggtgc cttgcccatt
      361 ccgatcaagg ttgttgaaag ccgcacagtt ggtcctaccc tcggtgagga aagtgtgcgc
      421 aagagcgtgt tggcaggtct cattggtttg accgttgtgg ttctcttcat gggcttttac
      481 tatcgcctcc ccggcgtcat tgcagacctt gcgttgattt gttacgccat tttctctttg
      541 atgcttttca agttgatccc tgtggtattg accttgcccg gtatcgctgg ctttattctt
      601 tctgtgggta tggcagtaga tgccaacgtg ttgatctttg aacgcttgaa ggaagaatta
      661 cgcgctggac gcaatctgcg tcacgccatc gatcttgctt ggagccgtgc ttggtcttcc
      721 attcgagact ccaacacatc gacactcatc acatgtgtca tcttgttcat tttcggcagc
      781 acctttggcg caagcatggt aaaaggtttt gcagttacct tggcgattgg cgtgctcgtc
      841 agcctcttca ctgccatcac cgtaacacgc accttcctgc atgccattct agacaatgtt
      901 aagaataccg aacaccccag ctggtttggg ctttaggaca ataccatgaa cataatcaag
      961 aatcgctatc tatactttct gatctccctg cttgtgatta tcccagggtt ggtcttcatg
     1021 ggactaaatt gggcaaacac gaaacagggt ccgttagttc ttggaattga tttccgaggc
     1081 ggctccttat tagagatcca gtttgaaggc actcgcccct catcttcaga cattgctgct
     1141 ctttacgccg aattctctac agaagaagaa aacttcaaag accccgttat tcaaccgctc
     1201 ggtgaagatg cctattccat tcgctcaaag accatgaccg atgaaaccaa aggcaagctt
     1261 gttgctgaaa tggaatctcg ttttgggtcc aaaatcactg tgttgaactt cacctctgtt
     1321 agcgcctctg ttggtgctga ggtgactcgt gctgcaggtg ttgctatttt catggctgca
     1381 gctgcgatcc tcatctatat ttggtgggcg ttccgaagtg tggaacacgc ctatcgctac
     1441 ggcacagcgg caatcattgc catgttgcat gatgtattcg ttgtgcttgg tgccgaagcc
     1501 attttgggct atttcttcgg ctgggaagca gatgcgctct tcctcactgc gctcctcacc
     1561 gtaatcggtt tttcagttca cgacacaatt gtggtgttcg acagagttcg cgaaaattcc
     1621 aatcttggtc gcaaaattgg ttatgaatcc atgatcaatc actccattgt ccaaaccttg
     1681 gaccgctctc tgaccaccca gttgaccgtt atgttcacgc ttttcgccct ctcgcttttt
     1741 ggtggcgata gcattcggca tttcaccatc atcttgttgg tgggtatctt aagcggtacc
     1801 tactcttcca ttttcaatgc ggcgcccatt cttgtcgttt gggaaaatcg cgaatggaca
     1861 acctggttcg gtcgaaaagc aagcgcgtaa aatacgcttc aaaacagcca tcaggtttga
     1921 tggctgtttt ttt
//
LOCUS       DAOQNJ010000535         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_51613_length_1930_cov_10.810133, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000535 DAOQNJ010000000
VERSION     DAOQNJ010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51613_length_1930_cov_10.810133"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..>1930
                     /locus_tag="PLG52_12625"
     CDS             118..>1930
                     /locus_tag="PLG52_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB42341.1"
                     /translation="MNNTSAASPQNNQFGLWANLPLSRKILLAFSVLFISNVIIAIIT
                     LQGMNRTQTAFEHAITQGLEIHALSDHLTISFLQARRAEKNFLLRWREEGFDTAYANY
                     IPLFDENVAAARADVKKLAAFGPVVATTGDYTQTQYNADIEALNQNISIYEQGFKAYV
                     DTLQQKGFDENTGFEGEFRTIARNIEGKISGGDGLEELEITLLQIRRNEKDYLLRTDQ
                     QYIDNVHSNISKLKTQIANTDKLTPTIKSDLLSYTDEYLTAFDSLIELDKKITIYNEN
                     LISSARSVEPLVAKIEGFGEQLATEDIDAARLNVSQTQTTSTAIVFGMLVISIFLAVG
                     LSRQITRPIIQLTSTAEVISTGNFDAQAKVDSSDEIGTLAQTFNTMTNRLNQALEDVR
                     RRALAVQTSAEVSQRLSVATDPQRLAVEVVEQVQAAFKYYHAHIYFLDEESGDLIMAG
                     GTGDAGATMLARGHKIPHGRGLVGRAAATNAPVLVPDVSQAEGWLPNPLLPDTKSEVA
                     IPISSGNQILGVLDVQQNIVNGLDEEDVRLLQSLAGQVAISLQNARSYEQSKSQAELE
                     TLANTIGQKIQRANTVEETLQTAIRELGLALGATRVKA"
BASE COUNT          578 a          503 c          411 g          438 t
ORIGIN      
        1 tgccttgcag gttgcagcac gtgaattggg gcatgccatg ggcatgaaat ccacagttgt
       61 aacgcttgaa cccaggtcaa tcgcaggtga aagcgaaggg aattaaggaa tctttacatg
      121 aacaacactt ctgcagcttc gccacaaaac aatcaatttg gcctttgggc aaacctccca
      181 ctatcgcgaa agatccttct tgcgttcagc gtattattca tatccaatgt gataatcgcc
      241 atcatcacat tgcaaggcat gaacagaaca caaaccgctt tcgaacatgc aattactcaa
      301 ggcctggaaa tccatgcact atcagatcac cttaccatca gctttttgca agcccgccgc
      361 gctgaaaaaa actttctgtt acgctggcgt gaagaagggt tcgacacggc ctatgcaaac
      421 tacattccct tgttcgatga aaatgttgct gcggcacgtg cagacgtaaa aaaactcgcg
      481 gcctttgggc ctgtagtagc cactaccggc gattacactc agacacaata taatgctgat
      541 attgaagccc tcaaccagaa catatccata tacgaacaag gttttaaggc ttatgtagat
      601 acactacaac aaaaggggtt tgatgaaaac acaggttttg agggagaatt cagaacaatt
      661 gccagaaata tcgaaggaaa aatttcaggc ggagatggtc tggaagagct ggaaattacc
      721 ctccttcaaa ttcgtcgcaa cgaaaaggac tatctgctgc gtacagatca acaatacatt
      781 gataatgtcc attcaaatat ttccaaacta aaaacccaaa tcgccaatac cgacaaactc
      841 acacctacca taaaaagcga tctactttct tatactgatg aatacctgac agccttcgac
      901 agccttatag agcttgataa aaaaatcacc atatacaatg aaaatttgat cagctccgca
      961 cgtagtgtag aaccactggt cgcaaagatc gagggatttg gtgaacaatt ggctacagaa
     1021 gatatcgatg ccgcacgatt gaatgtaagt caaacacaaa ccacttccac tgcaatcgta
     1081 tttggcatgc ttgtcatatc tatttttctg gcagtcggtc tttcccgtca aatcacccgc
     1141 ccgatcatcc aactcacaag cactgccgaa gttatttcaa ccggtaattt tgatgcacaa
     1201 gcaaaagtcg actcatcaga tgaaatcggt actctcgcac aaacattcaa caccatgaca
     1261 aaccgcctaa atcaggcact tgaagacgtc cgccgccgtg cattggccgt acaaacctct
     1321 gcagaggtca gtcaacgcct ctccgtagcc accgacccac aacgccttgc tgttgaagtg
     1381 gtggaacagg tgcaggccgc gttcaaatac tatcacgccc atatctactt cctcgatgaa
     1441 gaaagtggag acctcatcat ggcaggcggc accggcgatg caggcgcaac catgcttgca
     1501 cgtggtcaca aaatccctca tggccgtggt ttggtgggcc gcgccgctgc gaccaatgct
     1561 cctgtgttgg taccggatgt ctcacaagcc gaaggctggc tccccaaccc cttattacca
     1621 gataccaaat ctgaagtagc cattccgatc tcttcaggca atcagattct gggtgtgttg
     1681 gatgtgcagc aaaatatagt caatggtctt gacgaggaag atgttagatt actacaatcc
     1741 ctcgccgggc aggtcgcgat ctctttacaa aacgcacgct cgtacgaaca atccaaatcg
     1801 caggctgagt tggaaacgct ggcaaatacc attggccaga agatccaacg cgcaaacacc
     1861 gtggaagaga ccctacaaac cgccatccgc gaacttgggc ttgcattggg tgcaacccgc
     1921 gtcaaagcag
//
LOCUS       DAOQNJ010000536         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_51664_length_1929_cov_6.562433, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000536 DAOQNJ010000000
VERSION     DAOQNJ010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51664_length_1929_cov_6.562433"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="PLG52_12630"
     CDS             <1..1597
                     /locus_tag="PLG52_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNB42342.1"
                     /translation="YPDRACTIFKGAVISYREMNERSNAVAAALVEMGVKKGDRVGIF
                     MPNLPQFVSVYFGILKAGGVVIAVNPTYPVEEILTPVNDAGIEIMFTLSRFYGKVKEV
                     REKSGLKKIIVSNLKEALPPFTRLLFTLLKEKKDGDKLDSLDSRDVWLTDLLKKYAGA
                     SKPNVDIQPDDTALFQYSGGTTGVPKGAVAMHRNVVANTLQIKSWMTGLDEGKEVVLM
                     GIPLFHVYGMVAGMNFAMATGSSMVMIPNARDLKDVLENIAKYKATIFPGVPLLYNGI
                     NNHPDVKAGKYDLSSIKACISGSAALMRETKEEFERLTGGKVFEGFGMSEAPTAVTCN
                     PLNGVNKTGSIGMPLPDVDMKIISLDDGETEMQLGEIGEIVVNGPQVMKGYHNMPTET
                     ANSLRQLKDGKTWLFTGDIARMDEDGYFYIVDRKKELIKPGGFQVWPREVEEAIMDNP
                     KVLEVGVGGIPDPKSGETVKAWVVLKPGESMTEDELKEFCKERLAPYKVPRYVEFRTE
                     LPKTTVGKILRRELVRQHKEGGK"
     gene            complement(1660..>1929)
                     /locus_tag="PLG52_12635"
     CDS             complement(1660..>1929)
                     /locus_tag="PLG52_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNB42343.1"
                     /translation="QQEELIFLGREISNQRDYSEAVAEQIDREVRKLVEDAYKQARAT
                     VKKYRKQLDAVAKKLLEVESITREEFEQIFPPPLGKKSGTPQLTA"
BASE COUNT          489 a          451 c          524 g          465 t
ORIGIN      
        1 atacccagac cgtgcctgta cgatatttaa aggcgcggtc atttcgtatc gtgagatgaa
       61 cgaaagatcc aatgctgtgg cggctgcctt ggttgagatg ggtgtcaaga aaggggatcg
      121 ggttggtatt tttatgccca acctgccgca gttcgtaagc gtgtattttg gtattctcaa
      181 agcaggtggt gtggtgatcg ctgtgaatcc cacatacccg gtagaagaga ttctcacccc
      241 ggtaaatgat gccgggatcg agatcatgtt cacgctttca cgtttttatg gcaaggttaa
      301 agaagtacgc gaaaagtcgg ggcttaagaa aatcattgtt tccaatttga aagaagcttt
      361 gccgcctttc acccgtctgt tgttcactct tctaaaagaa aagaaggatg gcgacaaact
      421 ggactcgctc gatagtcgcg atgtatggct gacagatttg ctgaagaaat atgccggcgc
      481 ctcaaaaccg aacgtcgata ttcagccaga cgatacggcg ctcttccaat attctggcgg
      541 aacgaccggt gtgcccaagg gcgctgttgc catgcaccgt aatgtagtgg caaatacgct
      601 gcaaatcaaa agctggatga ccgggcttga tgaaggcaaa gaagtggtct tgatgggcat
      661 tccgctcttc catgtatatg gtatggtggc gggcatgaac tttgccatgg cgaccggttc
      721 cagtatggtc atgatcccga atgcgcgtga cttaaaagat gtattagaga atatcgccaa
      781 atacaaggcg accatcttcc ccggtgtgcc acttttgtat aacggcatca acaatcaccc
      841 ggatgtgaaa gctggcaagt atgacttgtc ctccatcaag gcttgtatct ctggttctgc
      901 ggcattgatg cgtgagacca aagaagaatt tgaacgcttg accggtggta aggtattcga
      961 agggttcggc atgtctgaag cccccaccgc tgtgacttgt aacccgctca acggcgtgaa
     1021 caagaccggc tctattggta tgcctttgcc cgatgtagat atgaagatca tcagtttgga
     1081 cgatggcgaa actgaaatgc aattgggcga gatcggcgaa atcgttgtga atggtccgca
     1141 agtgatgaaa ggctatcaca acatgccgac cgagaccgcc aactcgctgc gtcaactgaa
     1201 ggatggaaag acctggctct tcaccggtga tatcgctcgc atggatgaag atggctactt
     1261 ctatatcgtt gatcgcaaga aggaactcat taaaccgggc ggcttccaag tatggccacg
     1321 tgaagtggaa gaagccatta tggataaccc caaggtcctc gaagtgggcg tgggaggcat
     1381 cccagacccg aagagcggtg aaacggtcaa agcctgggtg gtgctcaaac ccggtgaaag
     1441 catgaccgaa gacgaattga aggaattctg caaagagcgc ctagcgccct acaaagtacc
     1501 gcgctatgtc gaattccgca ccgagttgcc caagacgact gtcggcaaga tcttacgccg
     1561 cgagttggtc cgccagcata aagaaggcgg caagtagttc gtaattaata aaataaaaga
     1621 gcctctcaaa ttgagaggct ctttttgtat gacagatatt tatgcggtca actgcggggt
     1681 gccgctcttc ttgccaagcg gaggcgggaa gatctgctca aactcttcgc gggtgatgga
     1741 ttccacttcg agcagtttct tggctactgc atccaactgc ttgcggtatt tcttcaccgt
     1801 cgcgcgagct tgcttgtacg catcttccac caacttgcgg acttcgcggt cgatctgttc
     1861 agccacggct tcagagtaat cacgctggtt ggagatttcg cgcccgagga agatcaattc
     1921 ttcctgctg
//
LOCUS       DAOQNJ010000537         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_51896_length_1924_cov_6.927234, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000537 DAOQNJ010000000
VERSION     DAOQNJ010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51896_length_1924_cov_6.927234"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..>1924
                     /locus_tag="PLG52_12640"
     CDS             61..>1924
                     /locus_tag="PLG52_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42344.1"
                     /translation="METVSTKKFLQVWLWALTVVSLLAILQTIQRTADLEIALLRSKW
                     IGLVGIFALTALFGAWFSFSALLNRISDWLDGFATKPLNSFRITFHISLIILGFLSIW
                     IVRLYIFGGTLPQVMPILWVFLWASLAQTIGLKALRRLDWHAAFSFVLLAQGFIYQTY
                     GIFAITSANPFSMGYSEAGRHYYASMFFAEKLYGMELPLPFLHPSRYLLLSIPFLAEG
                     LPLWFHRFWQAFLWFGLTLGSAWSLARFSRTSGWMKIFIIAWAFLFFFQGAVYYHLHV
                     MVILILAGVSIKHPGRSLIFIILASIWAGISRVNWFPVPAMLAIAIYVLETPIKDKGW
                     KYWLTPFIWGVSGLLAAIVSQFVYIQISGNADVSAFGSSFTSDLIWSRLLPNETFPMG
                     ILPGILLVSAPVLAALYQIVRGKTSALHSLRWLALLAMLAVLFVGGAIVSTKIGGGGD
                     LHNMDAYLVMLSVVVTAFWANQVSAESEAKSMMGRVNWGVVMAALLIPLGFAIRHIGF
                     YPIFDKTVAESDIQLLQETLKNGGEILFITERQLITFDLVDGVTLVPEYEQSELMEMA
                     MSRNRPYLENFYAELRARRFTLIVAEDQKFTQQKEGAFVEENVAWVRYVGAPLLC"
BASE COUNT          372 a          434 c          507 g          611 t
ORIGIN      
        1 cttcctgcct tccattttgg cagcgatgaa tgagaaaaaa gtagttgagt agtttgttgc
       61 atggaaactg tttcgacgaa aaaattcctt caagtttggc tatgggcgct gaccgttgtc
      121 agtttgcttg cgattctcca gacgattcaa cgcactgctg acttggagat cgcactcctg
      181 cgttcgaagt ggatcggctt ggtgggcatc tttgcattga cagccctctt cggcgcatgg
      241 ttttccttct ctgcattgtt gaatcgcata tcagactggc tggatggttt tgcaactaaa
      301 cctcttaatt cattccgaat cacgtttcac atttcactca tcattcttgg ttttctttcg
      361 atttggatcg tccgccttta catctttggc ggcacacttc cacaggttat gccaattctt
      421 tgggtgttcc tctgggcaag ccttgcgcag accattgggt tgaaagcgtt acgaagactc
      481 gattggcacg ctgccttttc ttttgttttg ctcgcacagg gatttatcta tcagacctac
      541 ggcatctttg cgatcacctc tgccaacccc ttctcgatgg gctattctga agccgggcga
      601 cattattatg cttcgatgtt ttttgcggaa aaattatatg ggatggaact cccgcttcca
      661 ttcctccacc cgtcgcggta tctgcttctc tcgatccctt tccttgcgga aggtttgccg
      721 ctctggttcc atcgcttttg gcaggcattt ttgtggttcg ggttgacgct tggctctgca
      781 tggagtcttg cccgtttctc ccgcacaagc gggtggatga aaattttcat cattgcctgg
      841 gcgttcttat tcttctttca aggcgcggtg tactatcact tgcatgtgat ggtgatcttg
      901 attctggctg gtgtatccat caagcatcca gggcgctcac tgatcttcat catcctcgct
      961 tcaatttggg cgggtatcag ccgtgtgaat tggttcccgg ttccggcgat gctggcgatt
     1021 gcaatttacg ttctcgaaac gccgatcaaa gataaaggct ggaagtattg gctcacgcct
     1081 ttcatttggg gtgtgagcgg acttctcgct gctattgttt ctcagttcgt ctatattcaa
     1141 atctcaggca atgccgatgt aagcgccttc ggttccagct tcacttcaga tctgatctgg
     1201 agtcgcttgt tgccgaatga aaccttcccg atgggaatct tgccagggat attgcttgtg
     1261 tctgcgccag tgttggcggc tttgtatcaa attgtgcgtg gaaaaacatc tgctcttcat
     1321 tcgttacgct ggctggcgct gcttgccatg cttgctgtgt tattcgttgg cggagccatc
     1381 gtcagcacga agatcggtgg cgggggcgac ttgcacaata tggacgcgta cctggtgatg
     1441 ctgtcggtgg tggtcactgc tttttgggcg aatcaagttt cggcggagtc agaagcgaag
     1501 tcgatgatgg gcagagtcaa ttggggtgtg gtgatggcgg cgttgctgat tccgcttggg
     1561 tttgcgattc ggcacatcgg cttttatcca atatttgata agacggttgc cgaaagcgat
     1621 attcaacttt tgcaagagac gcttaaaaat ggtggtgaga ttttattcat tactgaacgt
     1681 caactcatta cgtttgattt ggttgatggt gtgacgcttg tgcctgaata tgagcaaagc
     1741 gagttgatgg aaatggcaat gtcgcgtaat cgtccgtatc tcgaaaattt ttatgccgag
     1801 ttgcgtgcgc gccgttttac gttgatcgtt gctgaagatc aaaaatttac gcagcaaaaa
     1861 gaaggcgcgt tcgtggaaga aaatgtggcg tgggttcgtt atgtcggtgc gccgttgttg
     1921 tgta
//
LOCUS       DAOQNJ010000538         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_52107_length_1920_cov_10.167292, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000538 DAOQNJ010000000
VERSION     DAOQNJ010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52107_length_1920_cov_10.167292"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLG52_12645"
     CDS             complement(<1..524)
                     /locus_tag="PLG52_12645"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HNB42345.1"
                     /translation="MTETTAPARKSVPLWVQVTIWVFLAGLLALVGFGLNRASHPMAE
                     VGDTVPDFKLVYYEGYEHNNTPEMKLSDLRGKVVLINIWASWCKPCEQEAPELEQAWQ
                     LYKDQGDVVFIGVDYVDTPEGAFGYLKKFGITFPNAPDLQSNISSILNRQMGVPETYL
                     IDRDGVLRQIKIGP"
     gene            complement(557..1048)
                     /locus_tag="PLG52_12650"
     CDS             complement(557..1048)
                     /locus_tag="PLG52_12650"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNB42346.1"
                     /translation="MKKFLFILTLMLVASGLFTAVAFAQDKTPTDDEVNAIAKQLYCP
                     VCENTPLDVCPTEACHQWRELIRQMLAEGKSEQQIKDYFVANYGARVLSEPPRAGFNW
                     LVYIIPPVLILVGAVFLFNAFRAWTKPKSAEAGSGQAEEAGTSSSDDEYIKRLEEELK
                     NRK"
     gene            complement(1099..>1920)
                     /locus_tag="PLG52_12655"
     CDS             complement(1099..>1920)
                     /locus_tag="PLG52_12655"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNB42347.1"
                     /translation="FAGLLFLMAVAPLSAWGHSTVQTLGRAIWKSAALATLVTVALIF
                     TYTKNIYALIGFFAIALVLFATTHEFFRGAVARMNAQKENFFVAVGRLMGRNRRRYGG
                     YIIHISMALMAIGILGIEIFQQETQGTIAAGESLELGEYRVEYRELASWDEFNAGVNY
                     TRSVVDIYENGVFIGKLYPRIDYYYDSQQNMTIPGQHATLRDDLYILLVDWQPVSSEG
                     ATFKIYVNPLVNWLWIGSLTFLFGLIFAAWPDRDPADEPLRRKVSAGNRQTSAAD"
BASE COUNT          503 a          497 c          473 g          447 t
ORIGIN      
        1 aagggaccga tcttgatctg gcgcaagaca ccgtcgcggt cgatcaaata ggtttcaggt
       61 acgcccatct gacgattcaa aatgctggag atgtttgatt gaagatctgg cgcattgggg
      121 aatgtaatac caaacttttt caaataacca aacgcacctt cgggcgtatc cacataatcc
      181 acgccgatga aaacgacatc accttggtct ttatataact gccaagcttg ttcaagttca
      241 ggagcttcct gttcgcaggg cttgcaccat gaagcccaaa tgttgatgag cacaaccttg
      301 ccgcgcaaat cagaaagctt catctcaggc gtattattgt gttcgtagcc ttcgtaataa
      361 accaacttga aatctggcac ggtatcgccc acttctgcca tggggtggct ggcgcggttc
      421 aaaccgaaac ccaccaatgc gagcaaaccc gcaaggaaaa cccaaatggt gacttgcacc
      481 cacaagggga cgctctttct ggctggcgct gttgtttctg tcattgcatt ctccgtaaaa
      541 gaccgattgg tccctgttat tttctgttct tcaattcttc ttcaagacgc ttaatgtatt
      601 catcgtcact ggatgacgtg cccgcctctt ctgcttgacc tgaacctgcc tcagcggact
      661 tgggcttggt ccacgctcga aacgcattga agaggaagac ggctcccaca aggatcaata
      721 ctggtggaat gatatacacc aaccagttga acccagcccg tggcggctca gagagcacac
      781 gagcgccgta gtttgctaca aaataatctt tgatctgttg ctcactcttg ccttctgcca
      841 acatctggcg gatcaactcg cgccattgat gacaagcttc ggttgggcat acatccaacg
      901 gcgtgttttc acagaccggg caatacaatt gcttggcaat tgcattcact tcatcatcag
      961 taggggtttt gtcttgggca aatgccaccg ccgtgaagag accagaggca accagcataa
     1021 gggtaagaat aaagaggaat ttcttcattg aagttctccg agatcgatcg aaagttgatc
     1081 ttcgaccgat gactcttttt agtcggctgc actcgtttgg cgattgcccg cactcacctt
     1141 acggcgtaga ggctcatcag ccgggtcacg gtcgggccag gcggcaaaga tgagaccgaa
     1201 taagaaggtc aggctgccaa tccacaacca gttcaccaac gggttgacat agatcttgaa
     1261 ggtggcaccc tcgctcgaaa ccggctgcca atccacgagc aggatgtata agtcatcacg
     1321 caaagtggca tgctgaccgg ggatggtcat gttctgctgt gagtcgtagt aatagtcaat
     1381 gcgcggatag agtttgccaa tgaagacgcc gttttcgtaa atatccacaa ccgaacgagt
     1441 gtagttcaca cccgcattga actcatccca tgaagcgagt tcacgatatt ccacgcgata
     1501 ttcgcccaat tcaagcgact caccggctgc aattgtgcct tgagtttctt gttggaagat
     1561 ttcaatgccg agaataccga tcgccatcaa ggccatgctg atgtgaatga tgtagccacc
     1621 ataacggcgg cgattgcgtc ccatcaagcg acctacggca acgaagaagt tctctttctg
     1681 agcgttcatt ctggcaactg cgccacggaa gaattcatgg gtggtggcga acaataccaa
     1741 cgcaatcgcg aaaaacccga tcaaggcgta gatattcttt gtgtaggtga aaatcagagc
     1801 cacggtcacg agggtggcga gagcagcaga cttccaaatg gcacgaccga gagtctgcac
     1861 ggtggaatgt ccccaagcag agagcggcgc aactgccatc aagaagagca acccagcaaa
//
LOCUS       DAOQNJ010000539         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_52172_length_1919_cov_9.369635, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000539 DAOQNJ010000000
VERSION     DAOQNJ010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52172_length_1919_cov_9.369635"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PLG52_12660"
     CDS             <1..920
                     /locus_tag="PLG52_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNB42348.1"
                     /translation="TQPPTATVTSTPTQTLTPEPTLTPSITPTPTPLARRVLILSIDG
                     FRPDAIPLAPMPFLTSLLSQSAYSLTAQTVYPSVTLVSHTSMLSGQCPAKHKVDWNDY
                     IPENGYAQVTDLFDIVHAAGMQTVMYVGKEKLRQITEPQSTDIFKFINDRDLVITDNL
                     IENFPTDFRLMFVHFPTTDWMGHEYGWLSGEQLSVLFRADQALEKLVTELNNRGLMSE
                     TLFIITADHGGHATTHGTSLYEDMTIPWIAFGAGIKPAALNSSITTVDTAATAAFALG
                     LNIPTEWDGVPVYEAFGLPIEKASVGCSK"
     gene            941..1606
                     /locus_tag="PLG52_12665"
     CDS             941..1606
                     /locus_tag="PLG52_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB42349.1"
                     /translation="MERILEPELMEDMEQALAYSHADFATSHQMRVTWFQERFGKDNQ
                     PASILDLCCGGGDMTFRFARAFSHSRIIAVDGSQAMLDIAQKDVQAEPEIANRIQFVR
                     AFLPSDEIPQNKYDLVMSHSALHHFHDPNALWKTIRAHTRPGTLVFISDLQRTESVEA
                     AEQLVRERAGNEHPILQKDFAASLCAAFTPEEVREQLNANGLDALKVEAINDVYMLVY
                     GMM"
     gene            complement(1652..>1919)
                     /locus_tag="PLG52_12670"
     CDS             complement(1652..>1919)
                     /locus_tag="PLG52_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42350.1"
                     /translation="MAEMTCPKCSGAMEEGLQFNFKLNELSDDYVIETAELTRDRWQK
                     TEDATGKFFGKEYLGKRRVGPRLAVITYRCTVCGYLESYAPPVK"
BASE COUNT          499 a          545 c          398 g          477 t
ORIGIN      
        1 caacgcaacc tcctactgca acggtcacat caaccccgac gcaaactctc acaccagagc
       61 caacactaac accctcgatt acgcccactc ccacgcctct cgctcgccgc gtcttgattc
      121 tctccatcga cgggttccgc cccgatgcca ttccgctcgc cccgatgcct tttctcacat
      181 cactgttgag tcaatcagca tattcactaa ctgcgcaaac tgtatacccc agtgtgactc
      241 ttgtctcaca cacctccatg cttagcggac aatgccccgc caaacacaaa gtggattgga
      301 acgactacat tcctgaaaat ggctacgctc aagtgaccga ccttttcgat atcgtccacg
      361 ctgctggcat gcaaactgtt atgtatgtgg gcaaagaaaa gttacgtcag atcaccgaac
      421 cgcaaagcac agacatcttc aaatttatca acgaccgcga cctagtcatc accgacaatc
      481 tgatcgaaaa cttccccacc gattttcgtt tgatgttcgt tcacttccct accaccgact
      541 ggatggggca tgaatatggc tggctttctg gcgaacagct tagcgtgttg tttcgtgccg
      601 atcaggcgct tgaaaaactc gtgaccgaac tcaacaatcg cggtttgatg tctgaaactc
      661 tattcatcat cactgctgat catggcgggc atgccacaac gcatggtacc agtctctacg
      721 aagatatgac cattccatgg atcgcatttg gtgcaggcat taaaccagcc gcgctcaatt
      781 cgtctatcac cacagttgat actgccgcta ccgctgcctt tgccctcggg cttaacattc
      841 ccaccgaatg ggatggtgtg ccagtttatg aagccttcgg tttgcccatt gaaaaagcat
      901 ctgtgggttg cagtaaataa atttattaat agaggtcact atggaaagaa ttcttgaacc
      961 cgagctgatg gaagatatgg aacaagcact cgcctattca catgcagatt ttgccacttc
     1021 acaccaaatg cgtgtgactt ggttccagga acgttttggg aaggacaacc aacccgcctc
     1081 cattctggac ctgtgttgtg gtggcggcga tatgaccttt cgcttcgccc gagccttttc
     1141 acacagccgc atcatcgccg tggacggttc gcaagccatg ctcgatatcg ctcaaaaaga
     1201 tgtgcaagcc gagcctgaaa tcgcaaaccg gattcaattt gtgagagctt ttttacccag
     1261 cgatgaaatc ccccagaaca agtatgacct tgtaatgtcg catagtgcat tgcatcactt
     1321 ccacgacccg aatgctttat ggaaaaccat ccgcgctcac acccgcccag ggacattggt
     1381 cttcatctcc gatttgcagc gcaccgaaag tgtggaagcc gccgagcaac tcgtccgaga
     1441 acgagccggg aacgagcatc ccattctaca aaaagatttc gccgcttcgt tatgtgccgc
     1501 gttcacccct gaagaagtcc gcgagcaact caacgccaat gggctagatg cgctcaaagt
     1561 agaagccatc aacgacgtgt atatgctggt ttatggaatg atgtaaatta cataaaaaaa
     1621 cagctcaact aaagttcgat caggtagttt tttattttac aggcggagca taggattcta
     1681 aatatccgca taccgtgcat cgataagtaa taaccgccaa acgcggaccc acccggcgct
     1741 ttcctaaata ttctttccca aagaatttac ctgtggcatc ttctgtcttt tgccagcgat
     1801 cacgcgtcag ttcagccgtt tcgataacat agtcatctga tagctcattc aacttaaaat
     1861 taaactgtaa cccttcttcc attgcgccag aacattttgg gcaggtcatt tcagccatt
//
LOCUS       DAOQNJ010000540         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_52333_length_1916_cov_8.621709, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000540 DAOQNJ010000000
VERSION     DAOQNJ010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52333_length_1916_cov_8.621709"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1616)
                     /locus_tag="PLG52_12675"
     CDS             complement(153..1616)
                     /locus_tag="PLG52_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42351.1"
                     /translation="MQKTLRTSLLLFIAFFIGMAVIQFSTPNLPDNDGFYHIKMAWLM
                     RTEGLTPEFIWLPKSILNQNDFYDHHFLYHVALIPFTFGDLRVGAKLASVTFSALAFL
                     AVWVLFDRQKIPFAWLWALALLGISDAFLFRMSIPRAQSLSLGILALGLLWMFEGKYK
                     HLAVLSFFYVWTYNAFPLMIALAGLYTFAILLTEKRLDLRPVLWTSFGIFAGLVVNPY
                     FPKNLTFTYHHFLSKLQLGESIKVGNEWYPYDTAQVLSNSLLALVALFSGSMALGFSG
                     RKMTVQTVTAFLIALFFGIMMFNARRFVEYFPPFALIFSAFAWAPLFDNDGQPTQDWM
                     QKIPAGILALAILAGMVKSIPAVQHQLGTSKPYDLYAGASFWLLQNSEKGELIFQTDW
                     DDFPRLFYQNTYNTYLAGLDPTYFQLYNPELYNEWVSITQGDVANPSKNILSHFGSRF
                     VISDLNHGDFIQQAESDPNMREVYRDGQSVLFEIIEP"
     gene            1693..1899
                     /locus_tag="PLG52_12680"
     CDS             1693..1899
                     /locus_tag="PLG52_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42352.1"
                     /translation="MVITTVRIAAMVVIIVVIAVAIRVAIIIVIIAVRIAAMVIIICI
                     AIAQTLVRIAIGVSDAATKAGIVV"
BASE COUNT          576 a          431 c          438 g          471 t
ORIGIN      
        1 cacctacacg atacaacacg aagcgataga cttcagaaaa atactggtca tacacggccc
       61 caatggcttt agagtccaaa ccatccaggc ggctgatgtt ttgctgccca tttattgttg
      121 tcatctttga tgttccatac aacataatgg tatcacggct cgataatttc aaacaataca
      181 gactgcccat cgcgatagac ttcgcgcatg ttggggtcac tctctgcttg ctgaataaaa
      241 tcgccatggt tgaggtcaga gatgacaaaa cgtgacccaa aatgagaaag gatattttta
      301 gaagggtttg ccacatcccc ttgtgtaatg ctcacccact cattgtatag ctctgggtta
      361 tagagctgaa aatatgtggg gtcgagcccg gcaagatagg tgttgtaggt gttctgatag
      421 aacaaacgtg gaaagtcatc ccagtcggtt tgaaagatca gttctccttt ttctgaattc
      481 tgtaacagcc aaaaagatgc acctgcgtac aggtcatagg gtttggatgt tcccaattgg
      541 tgctgcaccg cagggattga tttcaccatg ccagcaagga ttgcgagagc cagaatccca
      601 gctggaatct tttgcatcca gtcttgagtt ggttgaccat cattatcaaa gagcggcgcc
      661 caagcgaatg ctgaaaatat taatgcaaat ggcgggaagt actccacaaa gcgacgagca
      721 ttgaacatca tgattccgaa gaataaagca atcaaaaatg cagttactgt ttgcaccgtc
      781 attttgcgac ctgaaaaccc tagcgccata cttccactaa acaaggcgac cagtgccaat
      841 aaggaattgc tcaatacttg ggctgtatcg tatggatacc actcattgcc aaccttgatc
      901 gattcaccta actgcaactt agacaagaaa tgatgatagg taaaggtcag gttcttggga
      961 aagtaggggt tgaccaccaa cccggcgaaa atgccgaagc tggtccacaa gaccgggcgg
     1021 aggtcgagcc gtttttccgt caaaagaata gcaaaggtgt acaaaccagc taaagcaatc
     1081 atcaatggga aagcgttgta cgtccaaaca taaaaaaagg aaagcacagc cagatgttta
     1141 tatttgcctt caaacatcca taacagaccg agtgccaaga taccgagtga aagggattgc
     1201 gcccgcggaa tggacatgcg aaacaaaaaa gcatctgaaa tgcccaacaa agccagcgcc
     1261 cacaaccatg caaaaggaat tttctggcga tcaaacaata cccacacagc aagaaaagcg
     1321 agcgcagaaa atgtaacact tgcaagtttg gcacccacgc gcagatcacc aaatgtaaaa
     1381 gggataagag ctacatgata gagaaaatgg tgatcgtaaa aatcgttctg gtttaagatg
     1441 cttttaggaa gccagataaa ctcgggagtc aagccttcgg tgcgcatgag ccaagccatt
     1501 ttgatatggt agaagccatc gttatctggc aggtttggcg tggaaaattg aataaccgcc
     1561 atgccaataa aaaaagcaat gaataaaagc agtgaagttc gaagtgtttt ttgcatagag
     1621 gtctttttta tatcaagtcg cccgggagaa acctgccggg cgacttttct attccttggc
     1681 tagtgaaaac taatggtcat cactaccgtt cgaattgccg ccatggtcgt catcatcgtc
     1741 gtcattgctg ttgccattcg agttgccatt atcatcgtca tcatcgccgt tcgaattgcc
     1801 gccatggtca tcatcatttg tattgccatt gctcagactc tcgttcgaat tgccattgga
     1861 gtttccgatg ccgctaccaa ggctggtatt gtcgtttaag ttaccgttgg catttc
//
LOCUS       DAOQNJ010000541         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_52450_length_1914_cov_8.802044, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000541 DAOQNJ010000000
VERSION     DAOQNJ010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52450_length_1914_cov_8.802044"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1244
                     /locus_tag="PLG52_12685"
     CDS             213..1244
                     /locus_tag="PLG52_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNB42353.1"
                     /translation="MNINFAMWRKASWQLIKMDDKKEWDALDVVSKWLIATRSAVTMV
                     TVYSCIIAGILAARDGKFDWLTFIIVTLGLFIAHGTNNILNDYTDFNRGVDRNNYFRT
                     EYGVHPLVQKFWDNKTSIRWFIVSGILATSAGVYALFHTGFSTTTIGLFAFGAVVLLA
                     YTYPFKYWGIGEFMIFLIWGPVLIGGVYFVLSGNWSWDVFVAGIPFGLSVASINIAKH
                     IDKHDPDKEKGVGTFPVRVGEANARRVNQVAIVLIYLVTLYLVLDGYFTPVMLLIFLA
                     YKEALMAIAVMNKPKPAQAPEGWPAWPVWFSGFAFQHNRKFGGLLIVALILDALIHYF
                     APTLWTGLF"
     gene            complement(1290..>1914)
                     /locus_tag="PLG52_12690"
     CDS             complement(1290..>1914)
                     /locus_tag="PLG52_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNB42354.1"
                     /translation="IDDYFGLPRKKFPHHALTLSLRARTSSSAIPGTWGFGLWNDPFG
                     MSLGFGANRFRLPTLPNAAWFFGASPENHLSFSDKPAQGFLAQSFCSPKFQASLIPAG
                     TLLPFSPKMTRRMLSKVIAEDSSALRVDVTQWHGYRLEWRSNRVAWYVDEAQVFEASV
                     SPNPPLGLVIWIDNQFAAFKPEGKISFGVLEGNEEWLEIEDLDMSSK"
BASE COUNT          446 a          462 c          473 g          533 t
ORIGIN      
        1 cgttttgccc atattgagaa aagcgccaaa ggacaggggc gaaacctttc agatatgacc
       61 cttgaagaga tggacgcctt ctggaacgag gcaaaacgcc tgggactcta aacctgtgca
      121 aaagttcccc ttttcacaaa agagtcgtat aatgcgtttt cgattgatta attaacggtg
      181 taagccgttt caatatctca aggagagagt atatgaatat caattttgct atgtggcgta
      241 aagcctcctg gcagttgatc aagatggacg acaagaaaga gtgggacgcg ctcgatgttg
      301 tatccaaatg gttaattgcc acccgttctg ctgtgaccat ggtcacagtt tattcctgta
      361 tcattgctgg gatattagcg gcgcgtgatg gaaagtttga ttggttgacc tttatcattg
      421 tcaccctcgg cttattcatt gcacacggca ccaacaacat cctcaatgat tacaccgact
      481 tcaatcgcgg cgttgacagg aacaattatt tccgcaccga atatggtgtt caccccctcg
      541 ttcaaaaatt ctgggataac aagacctcca ttcgctggtt cattgtgagt ggcattcttg
      601 ccacatcggc aggtgtgtat gcactcttcc acactggctt tagcactacc accattgggt
      661 tgtttgcttt cggtgctgtg gttttgctgg cgtataccta tcccttcaag tattggggca
      721 ttggcgaatt catgatcttc ctcatttggg gaccggtctt gattggtggc gtgtactttg
      781 tgctttctgg caattggagc tgggatgtgt ttgtggctgg tattcccttt ggtttgagcg
      841 ttgccagcat caacattgcc aagcacatcg ataagcatga ccctgataaa gaaaaaggcg
      901 tgggaacatt ccctgtccgc gtgggtgagg cgaatgcccg ccgcgtgaat caggttgcca
      961 ttgttttgat ctacttggtc actttgtacc tggtgctcga tggctatttc acccctgtaa
     1021 tgctcttgat cttccttgct tataaagaag ccctcatggc aattgccgtg atgaataagc
     1081 ccaagcctgc tcaggcgcct gaaggctggc ccgcttggcc cgtgtggttc tctgggtttg
     1141 ctttccagca caaccgcaag tttggtggtt tgctcatcgt cgctcttatt ctcgatgcgc
     1201 tgatccacta ctttgcgccc accctgtgga ccggtttgtt ctaaagcata aatgataaaa
     1261 aaataggctc caattttgga gcctattttt tatttgctac tcatgtctaa atcttcaatt
     1321 tctagccatt cttcgttgcc ttctaaaacc ccaaatgaaa ttttgccttc aggcttgaat
     1381 gccgcaaact gattatcgat ccagatgacc aaccctaagg gcggattcgg acttactgac
     1441 gcctcgaaca cttgggcttc atctacatac caagctaccc ggttcgatct ccactcgagc
     1501 ctgtatccat gccattgagt gacatccacc ctcagcgcgg acgagtcctc ggcaatgacc
     1561 ttgctcaaca tacggcgggt cattttgggt gagaaaggga ggagcgtccc tgcagggatg
     1621 agggatgctt ggaattttgg cgagcaaaaa ctttgcgcta gaaatccttg tgcaggttta
     1681 tcgctgaatg aaagatgatt ttctggcgat gccccaaaga accaagccgc attcggcagt
     1741 gtgggtagac ggaatcgatt cgcgccaaag cctagtgaca tgccgaaggg atcgttccac
     1801 aagccgaagc cccaggttcc ggggatggca gagcttgaag tccgcgcacg caggctcagg
     1861 gtcagagcat gatgcgggaa ttttttccga ggtagaccaa agtaatcatc gatc
//
LOCUS       DAOQNJ010000542         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_52640_length_1910_cov_7.685175, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000542 DAOQNJ010000000
VERSION     DAOQNJ010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52640_length_1910_cov_7.685175"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PLG52_12695"
     CDS             complement(<1..161)
                     /locus_tag="PLG52_12695"
                     /inference="COORDINATES: protein motif:HMM:NF013804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HNB42355.1"
                     /translation="MTDNIKIIANNRKAGFEYTLIETFEAGISLQGSEIKSIRAGQIS
                     IQEAYVDVE"
     gene            complement(234..1487)
                     /locus_tag="PLG52_12700"
     CDS             complement(234..1487)
                     /locus_tag="PLG52_12700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB42356.1"
                     /translation="MRFNLFKQSDIPEKYRSNFLHLYFDIAWFGVLSGTAVNFLNVYA
                     TRLGASAAQVGLLTAASAVVNLFLAIPAGHWISKRHTGMAVFWSSVLFRIGYFMWIPL
                     PWFLSNQGQIWALIILTFLMAIPLTPLGVGFNALFAEAVPDRYRARVAGTRNVTFAIA
                     YMLTSLVAGYILKNTEFPGGYQVIFVIGAFGAAMSSYHIYHVKPMQDDTPAAPSAPVP
                     ASAPKPDSPRGMTSALRLDIWKSPFKNVLLALFIFHFAHYLSNPLYPIFNVRVLNLND
                     SHLGNGTALYYLSVLLASTQLGRIVHRFGNKRITGWGVAGMASYPLMLAFAHTVWQFY
                     VLSFLGGFLFALVNGSYANYMLERIPADDRPSHLAWYTLMFNFAILTSSLIGPAVAEV
                     IDLVPAMIAFGIARILAGLFLLKWG"
     gene            complement(1554..1805)
                     /locus_tag="PLG52_12705"
     CDS             complement(1554..1805)
                     /locus_tag="PLG52_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNB42357.1"
                     /translation="MSQPNITVYGADWCGDCRRSKRFLTEKNIPFTWVDVDQDQAGEA
                     LVLEKNNGKRIIPTILFEDGTFLAEPSDEALADKLGLPV"
BASE COUNT          520 a          417 c          540 g          433 t
ORIGIN      
        1 ttttccacgt ccacataggc ttcttgaatg ctgatctgcc cggcacgaat ggatttgatc
       61 tcagatcctt gcagggaaat gcccgcttcg aaggtttcga tgagagtgta ttcaaaaccg
      121 gctttacggt tgttggcgat gattttgatg ttatctgtca caagatgatt ttagcatgga
      181 aaattgttgg ggaataagcc acagaacccg cagagatttt gagggctagt tgattagccc
      241 catttgagca ggaacaaccc agcgaggatg cgggcaatgc caaaggcgat catggcaggg
      301 acaaggtcaa tgacctctgc cacagccgga ccgatcaacg agctggtgag gatggcaaag
      361 ttgaacatga gggtatacca cgccaaatgc gaagggcggt catcggcggg gatgcgctca
      421 agcatgtagt tggcatatga accattgacc aaggcaaaca gaaagccgcc aaggaatgaa
      481 aggacgtaaa actgccaaac ggtgtgcgcg aatgccaaca tcagcgggta cgaagccata
      541 cccgccacgc cccagcctgt gatgcgtttg ttcccaaagc gatggacgat gcgccctaat
      601 tgagtggatg ccagcaatac agatagatag tagagcgcgg tcccattgcc gaggtggctg
      661 tcattgaggt tgagcacacg cacgttgaaa atcggataca gcggatttga caggtagtgt
      721 gcaaagtgaa agatgaacaa tgccagcaag acattcttga atggcgattt ccaaatgtcc
      781 aggcgcaggg cggaggtcat gccgcgggga gagtctggtt tgggggcaga ggcaggcacg
      841 ggcgcggaag gagccgcagg cgtatcgtct tgcatcggtt tgacgtggta gatgtgataa
      901 ctgctcatcg ccgcgccaaa ggctccgatc acaaagatga cttgataccc acctgggaat
      961 tcggtgttct tgagaatgta accagcaacg agggaggtga gcatgtaggc aatggcgaag
     1021 gtcacattgc gagtgcctgc cacgcgggcg cgataacggt ctggcactgc ttcggcaaag
     1081 agtgcattga accccacgcc taatggcgta agtggaattg ccatgagaaa ggtcaaaatg
     1141 atgagcgccc aaatttgccc ttggttgcta agaaaccaag gcaggggaat ccacatgaaa
     1201 tagccaatgc gaaagagcac cgaagaccaa aagaccgcca taccggtgtg acgtttgctg
     1261 atccaatgcc cggcaggaat agcaaggaaa agattgacga cggcagaggc tgctgtaagc
     1321 aagccgactt gcgcagcgct cgcccccaag cgcgtggcat acacattgag aaagttaacg
     1381 gcggtgccgc ttagcacgcc gaaccaggca atatcgaaat agagatgaag aaagtttgat
     1441 ctgtattttt caggaatatc tgattgttta aataaattga aacgcataca tgaaattata
     1501 ctcacgtatg cgtttcgaga ttttgcagct tatttgattt tcaggttgtt ttgttagacg
     1561 ggtagaccaa gtttatctgc tagagcttcg tcagatggtt cagcaagaaa ggtgccatct
     1621 tcaaaaagga tggttggaat gatgcgtttg ccgttgttct tttccaaaac aagcgcttca
     1681 cctgcttggt cttggtcgac atcgacccat gtgaagggga tatttttttc ggtcaaaaag
     1741 cgtttagagc gccgacagtc gccgcaccag tctgcgccat agacggtgat gttgggttgt
     1801 gacatgaatt cctccggtga tgaaaactga aatgagtgta cttccgctac cgtcgcgctg
     1861 tcagtgctat taattacatt ttgggaggaa taaaattaca atgtcttcat
//
LOCUS       DAOQNJ010000543         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_53193_length_1899_cov_8.939805, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000543 DAOQNJ010000000
VERSION     DAOQNJ010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53193_length_1899_cov_8.939805"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLG52_12710"
     CDS             complement(<1..611)
                     /locus_tag="PLG52_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNB42358.1"
                     /translation="MDYKDYYKTLGVDRKASTDEIRTAYRKLAMKYHPDKNPGDKKAE
                     DKFKEINEAYQVLSDEQKRAHYDRLGSAYSNYRSTGGRPGDFRWDDWYQQQAAGQRGS
                     VNMEDLFGGSGGGGFSDFFRTIFGEAMRGQGRGNPFVQDSPGYEQEVSISFQEAFDGT
                     TRQLQKQDGKKLTVRIPAGVKTGSKVRVAGAGPDGSDLYLLIQ"
     gene            735..1319
                     /locus_tag="PLG52_12715"
     CDS             735..1319
                     /locus_tag="PLG52_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNB42359.1"
                     /translation="MMKSVCVFCGSSDAVHPSYKKAAFQMGVTLAQNGIQIVYGGGKT
                     GLMGELANGALSVGGAVIGVIITSMNTPALAHTGITRMEVTAEMRSRKARMHELSDGF
                     IAMPGGFGTWDELFETLTWAQTGEHEKPVGLFNVNNFYAPLLAAIDHAVAEGFIFSEH
                     RQAISLETDPAKLIESMRNYEHPHDAVKRWMKAE"
     gene            complement(1402..>1899)
                     /locus_tag="PLG52_12720"
     CDS             complement(1402..>1899)
                     /locus_tag="PLG52_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNB42360.1"
                     /translation="IQDGELINIDVSAELNGYFGDTGASMVVGTPNPEYQKMLDATKA
                     TLDKVLTLVRAGKPLNIIGKTVQQEANKNGYNPIFDLTGHGIGRQLHDKPSAVYNFHK
                     PDDRRTLDNGLVLAIEPFFTTGQGHVVEKSDGWSLKTVDNTIAAQFEHTVIITKGEPI
                     ILTLP"
BASE COUNT          470 a          473 c          466 g          490 t
ORIGIN      
        1 atctggatca acagataaag atcgcttcca tctggacccg cacctgccac gcggaccttt
       61 gaaccagtct tcaccccagc cggaatacgg acggtcaatt tcttgccatc ctgcttctgt
      121 agctgacggg tcgtgccatc aaaggcttct tgaaaactga tgctaacttc ttgctcatac
      181 cccggtgaat cttgtacgaa tggattccca cgcccttgtc cgcgcatggc ttcgccaaag
      241 atggtgcgga agaaatcaga aaatccgccg ccacccgaac cgccgaacaa gtcttccata
      301 ttcacactac cgcgctgacc cgccgcctgt tgttgatacc aatcatccca acggaaatca
      361 cccggtcgcc caccagtgga acgatagttg gagtaggcgc tgccaaggcg gtcgtaatga
      421 gcgcgttttt gttcatcgct caaaacctga taagcttcgt tgatctcctt gaacttatct
      481 tccgcctttt tatcgccagg gtttttgtct ggatgatatt tcatggcgag tttacgatat
      541 gccgtgcgaa tctcatccgt gctggctttg cggtctacac caagggtttt atagtagtct
      601 ttatagtcca tgacgctatt ttcatcccgt caagggacag ggtcaagcgt ccaaaggttg
      661 cacttacata actctaacct gtctgtagtg tggtagagtc agcgaaaatt attctaagaa
      721 gacaaaatga gggcatgatg aaatcagttt gtgtattttg tgggtcttca gatgccgttc
      781 acccctccta caaaaaggcg gcatttcaaa tgggcgtgac ccttgcccag aatggcattc
      841 aaattgtcta tggtggcggc aagaccgggc tgatgggcga actagcgaac ggggctttat
      901 ccgtaggtgg cgctgtgatc ggcgtaatta tcacctcaat gaacaccccc gcattagcgc
      961 ataccggcat cacccgcatg gaagtaaccg ctgaaatgcg atcacgcaag gctcgtatgc
     1021 atgaactatc ggatggcttt attgcaatgc ccggcggctt tggcacgtgg gatgaactct
     1081 tcgaaaccct cacatgggca caaaccggcg aacatgaaaa accagttggg ctgttcaatg
     1141 tgaataactt ttacgcgccc ctacttgctg ccatagacca tgccgttgca gaaggcttta
     1201 ttttttcaga acatcggcag gcaatttcac tggagaccga ccccgctaaa ctgatagagt
     1261 ctatgcggaa ttacgaacac ccgcatgatg cagtgaaacg atggatgaag gctgaataag
     1321 gatcttggat taaacaattt acgcatcagg taatacgaga tttgtattgc ctgatgcgta
     1381 atgatttaac aagcgtgttt tctatggcaa cgtcaaaata atcggctcgc ctttggtgat
     1441 gatgacggta tgctcaaatt gagccgcaat cgtattatcc acggttttca aagaccagcc
     1501 atcagacttt tcgacgacgt gtccctgccc ggtggtaaag aaaggctcaa tggcgaggac
     1561 caagccatta tccagtgtgc ggcggtcatc tggcttgtga aagttataga ccgcggacgg
     1621 cttgtcgtgc agttgacgtc caatgccatg cccggtcaaa tcaaagatgg ggttatagcc
     1681 gttcttgttg gcttcttgtt gaacggtctt gccaatgatg ttgagcggtt tgccagcgcg
     1741 gaccaaggta aggactttat ccaaggtggc tttggtggca tccaacattt tttgatattc
     1801 agggttgggc gtgcccacga ccatagaagc accagtatct ccaaagtagc cattcagctc
     1861 agcagagaca tcaatgttga tcagttcgcc atcttgaat
//
LOCUS       DAOQNJ010000544         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_53470_length_1894_cov_7.015769, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000544 DAOQNJ010000000
VERSION     DAOQNJ010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53470_length_1894_cov_7.015769"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1272)
                     /locus_tag="PLG52_12725"
     CDS             complement(10..1272)
                     /locus_tag="PLG52_12725"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNB42361.1"
                     /translation="MTGTTHQQMLAKYAEAIVKVGLNIRAGQRLIITAAGTRGVPNQF
                     APLVREITKAAYGVGAKYVDVIFADEEMMRIRAQHAPKGSFGEYSKWQIQGILDMINN
                     GDALLSIGGSNPDLLGGLDPEVVGQMQRAHLENWSPISEKVTTNAINWCVVAAAGEAW
                     AHKIFPDLPLNQAQEKLWQAIFETTRIDQPDPVGAWSKHIASLRERALYLQSKQYTAL
                     HYTAPGTDFTVGLPSGHLWISAQSMAQNGVAFTANMPTEEVFTLPDRNRADGVISATF
                     PLSYGGTLIENFQVHFENGRITKVTAKKGEAALQKLVDTDEGSHRLGEIALVPASSPI
                     GKRGHLFYNTLYDENASCHIAIGRAYRFTLAGGTELNDDEFTAAGGNISLNHVDFMIG
                     SPQMDIDGITKDGKHEPVMRQGEWAISL"
     gene            1431..>1894
                     /locus_tag="PLG52_12730"
     CDS             1431..>1894
                     /locus_tag="PLG52_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42362.1"
                     /translation="MLVIEKVDTENKKQVKRFVELPPRLYADCPQWVPPLNVDAYNQL
                     NRKKHPFHEHSDVDFFLAVRDGLDVGRIAVIENKPFNQYHQEKTADFYLFECEDDLEA
                     ATALFNTAFEWAKSRGLTKMVGPKGMGPLDGYGVLVFGHEHRQTMTMLNYNHA"
BASE COUNT          466 a          425 c          506 g          497 t
ORIGIN      
        1 ttgattcggt tacagtgaga ttgcccattc accctgacgc atcaccggct catgtttgcc
       61 gtctttggta atgccatcaa tgtccatttg cggagaaccg atcatgaagt caacgtggtt
      121 gaggctgatg ttgcctccag ccgcggtaaa ttcatcgtcg ttcaactcag ttcctccggc
      181 gagagtgaag cggtaggcgc gtccaatggc aatgtgacag gaggcgtttt catcataaag
      241 cgtgttgtag aagagatgtc cgcgtttgcc aatgggggat gatgctggga cgagggcgat
      301 ttcaccgagg cgatgtgaac cttcatcggt atccactaat ttttgaaggg cggcttcacc
      361 tttcttggct gtgaccttgg taatacgccc attctcaaag tggacttgga agttttcgat
      421 caaagtgccg ccatagctga gcgggaaggt ggcagagatc acaccatctg cacggttgcg
      481 atctggcaga gtaaagactt cttcggtagg catattagca gtaaaagcaa caccattctg
      541 cgccatcgat tgggcactga tccagagatg tccgctgggc aagccgacgg taaagtcggt
      601 gccgggggct gtgtaatgca acgctgtgta ttgcttggat tgcagataca aagcccgttc
      661 gcgtaaagag gcgatgtgtt tgctccatgc accaacgggg tcgggttggt caatacgagt
      721 tgtttcaaag atggcttgcc aaagtttttc ttgcgcttgg ttcagaggaa ggtctgggaa
      781 aatcttatgc gcccatgctt caccagcggc ggcaactaca caccagttga tggcattggt
      841 ggtcactttt tcactaatgg gactccaatt ttccaaatgg gctcgttgca tctgcccaac
      901 aacttcaggg tcaagcccac caagtaggtc ggggtttgag ccgccgatgg aaagcaaggc
      961 atcgccattg ttgatcatgt ccaagatgcc ttgaatttgc catttggaat attctccaaa
     1021 gctgcctttc ggggcatgtt gagcgcggat gcgcatcatt tcttcatctg caaagatgac
     1081 atctacatat tttgcgccaa caccatacgc ggctttggta atttctctta ctaatggtgc
     1141 aaattggtta ggcacaccgc gcgtccctgc ggctgtgatg atcaaacgtt gcccggcgcg
     1201 aatatttaga cctactttta caatggcttc ggcatatttt gccagcattt gttggtgggt
     1261 agttcctgtc ataaatcctc agttaatttg ggagatagtt acaagtatag catggcgaca
     1321 ggggtttttg cacgagggat atccattaca aaagcaatgc acacccacct tttaaaatgg
     1381 ggtggaaggg atatacttgg ctgaaaccta gcctctatag gagcaaaacc atgcttgtta
     1441 tcgagaaagt ggataccgag aataaaaagc aggtcaaacg gttcgtggag ttaccacccc
     1501 gcctctatgc ggattgcccg cagtgggtgc cgccattgaa cgtggatgcg tacaatcagc
     1561 tcaatcgaaa gaagcatccc ttccatgaac attctgatgt ggattttttc ctagcggttc
     1621 gtgacggact ggatgtaggg cggattgctg taattgaaaa caaacctttc aaccaatatc
     1681 atcaagaaaa gacggcagat ttttatttat tcgagtgtga ggatgacctt gaagcggcta
     1741 cggcattgtt caacacagcg tttgagtggg caaaatcacg cggattaacc aagatggtcg
     1801 gaccgaaagg gatgggtccg ttagatgggt atggagtgtt ggttttcggg catgaacacc
     1861 gtcagaccat gaccatgctc aattacaacc atgc
//
LOCUS       DAOQNJ010000545         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_53689_length_1889_cov_9.301527, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000545 DAOQNJ010000000
VERSION     DAOQNJ010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53689_length_1889_cov_9.301527"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PLG52_12735"
     CDS             complement(<1..1197)
                     /locus_tag="PLG52_12735"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB42363.1"
                     /translation="MNIYISLLQLRPKYRLLWLAQVISLAGDWFNTIASVIIVNRYTT
                     SGLALGGLFIARALPPFLLGPVAGVVADRFDRRKVLIASDILRAVIVLCFLLVDRPER
                     LWLMYVLTVLQFSVSTFFEPARAALTPALVETDELLNANTLASITWSAMLTLGGAIGG
                     VTASYFGVPVSLIVDAASFIVSAILVASITGEFRSQMAHALESGWKNFIDGMNYVRKN
                     LDVGFVTLVKGFGQVGSFDIIAALYAARLFPYGQEGATTLGLMFTAFGLGAVIGPLIA
                     NRFGDASANWLRRAILGGFVFMPLAWFIIGTATSFPIVLIGCIIRGMGGSINWTYSDV
                     LLQMTVPDHFLGRVFAFDFGVYTLAVSVSLWLTGSAIDEFNFAPRTIVLILAAGSLLP
                     LAAWSAA"
     gene            complement(1312..1863)
                     /gene="msrA"
                     /locus_tag="PLG52_12740"
     CDS             complement(1312..1863)
                     /gene="msrA"
                     /locus_tag="PLG52_12740"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNB42364.1"
                     /translation="MNTTYETVTLAGGCFWCLEAVYDEVKGVLSVDSGYANGHVKNPS
                     YRAVCNGDTGHAEVVQIKFDPSVITFRDLLNIFFVIHDPTTLNRQGADVGTQYRSGIY
                     YHMPEQKEVAEQTIRELEAQKVWGSRIVTEVEPIKDFYIAEDYHQEYFVRNQSQPYCQ
                     AVVAPKVGKFRKHFLELWKKQAA"
BASE COUNT          549 a          513 c          432 g          395 t
ORIGIN      
        1 agcggcgctc cacgcagcca gagggagcag gctgcctgcc gccagaatta agacgatcgt
       61 acgcggggca aaattgaatt catcgatcgc agagccagta agccagagcg acaccgaaac
      121 cgccagggtg tacacgccaa aatcgaaagc aaaaacacga ccgagaaaat gatcggggac
      181 ggtcatttgc aaaaggacgt cactgtacgt ccagttgatc gagccaccca tcccgcgtat
      241 gatgcacccg atcaatacaa tcgggaaaga tgttgccgtg ccgataatga accatgccag
      301 cggcatgaat acaaatccgc caagaatggc gcgacgcagc caatttgcgc tcgcgtcacc
      361 aaaacgattc gcaatgagcg gaccgatcac agcgcccaag ccaaaggctg taaacatcag
      421 ccctaatgtg gtcgctccct cctgcccgta gggaaagagg cgagcagcat acaaggcagc
      481 aatgatgtca aagctgccta cttgtccaaa ccctttcaca agggtcacaa atcctacatc
      541 cagatttttg cgaacatagt tcatgccgtc gataaagttt ttccagccgc tctccaatgc
      601 atgcgccatc tgcgaacgga attcgccggt gatgctggca accaatatgg ctgaaacaat
      661 aaaactagcc gcatccacaa tgagtgaaac aggcacgcca aaataagaag ctgttacccc
      721 accgatagca ccacccaacg taagcatcgc agaccaagtg atactcgcca aggtattggc
      781 attgagaagt tcatcggttt caaccaaggc tggggtgagc gctgctcgtg ccggttcaaa
      841 aaaagtagaa acactaaatt gcaagacggt gagcacatac ataagccata gccgctcagg
      901 tcggtccacc agcagaaagc aaagcacgat cacggctcgc agaatatcac ttgcaatcag
      961 cactttgcgt cggtcaaagc ggtcagctac aactccggca acgggtccca gcaaaaatgg
     1021 cggaagcgca cgggcaatga ataaccctcc gagcgcaagc ccggatgttg tatagcgatt
     1081 aacaatgatg accgaagcga tggtgttaaa ccaatcgcct gcaaggctaa tgacctgtgc
     1141 cagccaaagc aatctatatt ttggacgaag ttggagaagg gagatgtaga tattcactca
     1201 ggcaattata gccgtgagaa caatcattcc acttcgattt gaaattcgaa taaataaaaa
     1261 gggacgcagc tcagtattca actgcgtccc tttttatatg atgcttacga actacgctgc
     1321 ttgcttcttc cacaattcca agaagtgttt gcggaactta ccaaccttcg gtgcaaccac
     1381 tgcctgacaa tatggctgag attgattccg cacgaaatat tcctgatggt aatcctcagc
     1441 aatatagaaa tccttgatcg gctccacttc cgtgacgatg cggctgcccc agaccttttg
     1501 cgcttccaac tcgcggatgg tctgctcggc aacttccttt tgttcgggca tgtgatagta
     1561 aatacccgag cggtattgtg tgcccacatc cgcaccctga cggttcaatg tggtcgggtc
     1621 gtggatgaca aaaaagatat tcaacaagtc gcgaaacgta atgacggacg ggtcaaactt
     1681 gatctgcacc acttctgcat gacccgtatc accattacac acggcgcgat aactcgggtt
     1741 cttcacatgc ccattggcat agcccgaatc cacagacaac acacccttca cttcatcata
     1801 aacggcttcc aaacaccaga aacatccccc tgctagcgta acggtttcat aagttgtatt
     1861 cataataagt tctccttttt accctgctt
//
LOCUS       DAOQNJ010000546         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_53741_length_1888_cov_8.587016, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000546 DAOQNJ010000000
VERSION     DAOQNJ010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53741_length_1888_cov_8.587016"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLG52_12745"
     CDS             complement(<1..842)
                     /locus_tag="PLG52_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42365.1"
                     /translation="MEEQNNTNHSPYAGRWVALVRGRVVAQGGTPEQALRASQSSRYK
                     ERPEIRFMSVPFNLPPLLEKIVNVLPANTEIYLVGGAVRDLLTNRRSPDFDFAVPSNG
                     ISLARTVANALKADFMVLDDERDTGRVIIIEDGTYTYLDFAAYRGATLEEDLRDRDFT
                     INAIALNLRDITVIDPLNGATDIRAKVIRACSPDSLSNDPVRILRAVRQAAAFGFSID
                     KSTREWMKQAADKINNVSIERVRDEVFKMLGGPKASASIRALEMLGVLQHLMPELLKM
                     KGVT"
     gene            complement(864..1421)
                     /gene="hpt"
                     /locus_tag="PLG52_12750"
     CDS             complement(864..1421)
                     /gene="hpt"
                     /locus_tag="PLG52_12750"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNB42366.1"
                     /translation="MQNYQDFLAEVLIDTASLQKRVAELGKQISADYNGQDLLLICIL
                     RGAVPFMVDLSRHITVPHMMDFMAVSSYGIGRRESSGSARVTLDLQMDIRDHNVLLVE
                     DIVDSGHTIAAVLQMLGTRQPKSLKVCALLDKPERREAHVPIDYLGFSIPNKFVFGYG
                     LDLDEYHRNLNFVGVVDLDRYKPVQ"
     gene            complement(1491..>1888)
                     /locus_tag="PLG52_12755"
     CDS             complement(1491..>1888)
                     /locus_tag="PLG52_12755"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNB42367.1"
                     /translation="STFDAIPVEKASEILSKVAADTTVVGLDEAQFFDAEIVDVAQTL
                     SDRGIRVIVAGLDMDFRGEPFGSMPVFMAKAESVLKLHAICMVCGDEASRTQRLVNGK
                     PARYDEPVVIVGASELYEARCRLHHEVPR"
BASE COUNT          517 a          473 c          457 g          441 t
ORIGIN      
        1 tgggtaacgc ccttcatttt caacaattca ggcataaggt gttgtaaaac gcccaacatc
       61 tctaacgccc gaatggacgc actggctttg ggtccgccaa gcattttaaa gacttcatct
      121 cgtacacgct caatagaaac gttgttgatc ttgtctgccg cttgtttcat ccactcgcga
      181 gtggatttgt cgatactgaa accaaatgct gccgcctgac gcacagcacg caaaatccgc
      241 accgggtcat tggacaagga atcaggtgag caggcacgaa tcactttggc gcggatatct
      301 gtcgcaccat tcaatgggtc tatgacagta atgtcacgta gattaagcgc aatcgcattg
      361 atggtaaagt cgcggtcgcg aaggtcttct tcgagtgtcg ctccacgata ggcagcgaaa
      421 tcgagatagg tatatgtacc gtcttcgata ataatgacac gtcccgtatc acgttcgtca
      481 tcgagaacca taaagtccgc tttaagggcg ttggcaacgg ttcgagcgag agaaatgcca
      541 ttcgaaggca cggcaaaatc gaagtcggga gagcggcggt tggtcaatag atcccgcact
      601 gccccaccga caagatagat ttccgtgttt gcaggaagga cattaactat tttttccaat
      661 aaagggggaa ggttgaaggg aacagacata aaacgaatct ctggtctttc tttatagcgg
      721 ctggattgcg aagcacgcaa ggcttgttct ggtgtaccgc cttgagcaac gacacgccca
      781 cgaacgaggg caacccaacg ccctgcatat ggtgaatgat tggtgttgtt ttgttcttcc
      841 aagatacccc ctgcaccggg cgcttattga acgggtttgt aacgatcgag atctacgacg
      901 ccaacaaaat ttaaattgcg gtgatattca tcaaggtcga gcccataacc gaagacgaac
      961 ttgttgggaa tactaaagcc aaggtaatca ataggcacat gcgcttcgcg acgttcgggc
     1021 ttatcgagca aggcgcaaac ctttagcgat ttgggctggc gtgttccaag catttgcaaa
     1081 acggcagcaa tggtatgacc actatccacg atatcttcaa caagcaaaac attgtggtca
     1141 cgaatatcca tttgaaggtc taaagtaaca cgagcagaac cgctagattc acgccttccg
     1201 attccatatg aagagacagc catgaagtcc atcatatgtg gcacggtgat gtggcggctc
     1261 aagtccacca taaaaggcac cgcgccgcgc agaatgcaga tgagcaacaa atcttgtccg
     1321 ttatagtcgg cactgatctg cttgcccagt tcggcaacgc gtttctgtaa agaagctgta
     1381 tcgataagaa cttcggcgag gaaatcctgg tagttttgca tcaaagtatt ccttgggtat
     1441 ttgagtttaa ttagtttatc actactaaag cccagtgtgg ggcttctcca ctaacgcggg
     1501 acttcgtgat gcaaacgaca gcgggcttca tacaactcag aagcaccaac gatgaccacc
     1561 ggctcatcat aacgggcagg cttgccattc accagacgct gagtcctaga cgcttcatct
     1621 ccacaaacca tacaaatggc atgcagcttc aacacagatt ctgcctttgc cataaacacc
     1681 ggcatggagc cgaatggttc gccacgaaaa tccatatcca aaccagcaac gatgacacga
     1741 atgccacggt ctgaaagggt ctgcgccacg tccacgatct cggcatcaaa gaactgcgcc
     1801 tcatccaaac ccacaacggt tgtgtctgct gcgacttttg agagaatttc actggctttt
     1861 tccacgggaa tggcatcgaa ggttgagc
//
LOCUS       DAOQNJ010000547         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_53742_length_1888_cov_6.762684, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000547 DAOQNJ010000000
VERSION     DAOQNJ010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53742_length_1888_cov_6.762684"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PLG52_12760"
     CDS             <1..992
                     /locus_tag="PLG52_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB42368.1"
                     /translation="VPFLGFAAVALLLPVVGVNAGLLLTFLLLIWQGLGGGFTANAWT
                     SMVSKIIPPESRGTFFGMQAGLANLFISGSAIAAGYLLNALASPWDFAVCFFIASIFF
                     ALSWFALAATREPEDTEKFIPEEKSHFWDDAKKVLSRDTNFNWFLIARILSQFATMGF
                     SFYIIYALREFNMTEVVAGYLTATLTISQTIANIGMGWIGDRVGHRVMLIIGAFSAFL
                     SALLAWNAASIAWFYPIFILTGLANVSMWTISMTMTVDFGNETDRPIYIGLSQTLTAP
                     ATIIAPILGGWIADSAGFVPTFTISTILSLVMVGILIFLVKDPRTSKLVNLQS"
     gene            1008..>1888
                     /locus_tag="PLG52_12765"
     CDS             1008..>1888
                     /locus_tag="PLG52_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNB42369.1"
                     /translation="MSLIQEKTDQAVQILKEQDIDLWLTFVRETSGVRDPVLSFIIGE
                     ADLTWPSALIMTKTRDKIAIVGNLETEALQRLDVFTQIIGYDTGVSGVLRETISKLNP
                     NQIAVNMSRNNVHADGLTHAMYEFLVEYLSGTPYPERIISAEPVINALRGRKTKAELA
                     RIQRAVEITDEIYKKTFAFIKVGMTEIQIADYMQALMKEYGVGYAWPRENNPAVNSGP
                     DKVVGHNGPTDIKVEPGHIIHFDFGVKYEGYCSDIQRIAYVLREGETEAPVEVQRGFI
                     TVRTAIEKSREAMKAGV"
BASE COUNT          463 a          511 c          444 g          470 t
ORIGIN      
        1 gtgttccatt tttagggttt gcagcagtag ctctcttact ccccgtggtt ggcgtgaatg
       61 ccggtctgct gcttacgttt ttgctactga tatggcaagg gcttggcggc ggttttaccg
      121 ccaatgcgtg gacctcaatg gtctccaaga tcattccacc ggagtcacgt ggcactttct
      181 ttgggatgca agccggactg gcgaatctct tcatcagcgg atctgccatc gcagcgggat
      241 acctacttaa cgccctcgcc tctccctggg actttgcggt ctgtttcttc attgccagca
      301 ttttcttcgc cttatcctgg ttcgccctag ccgctacccg cgaacctgaa gataccgaaa
      361 agtttatccc cgaagaaaaa tctcatttct gggacgatgc caaaaaggta ttgagccgcg
      421 ataccaactt caactggttc ttgatcgcac gcatcctctc acaatttgcg accatgggat
      481 tctcgttcta cattatctac gccctacgcg aattcaatat gaccgaagta gtggcgggat
      541 atctcaccgc aacccttacc atttcacaaa cgattgccaa cattggcatg ggctggatcg
      601 gtgaccgggt tgggcatcgc gtcatgctta tcattggcgc attctctgca ttcctaagcg
      661 ctttacttgc gtggaatgcc gcttcaattg catggtttta ccccatcttc attctcactg
      721 gcttggcaaa tgtctctatg tggaccatca gtatgaccat gaccgtagac ttcggcaacg
      781 agacagaccg cccgatttac atcggcttat cccaaacact caccgccccc gcgaccatta
      841 ttgcccccat cctcggcggc tggatcgcag atagcgccgg gtttgtacct acatttacga
      901 tctcaaccat cctttcattg gtgatggtag gcattttgat tttcctcgtc aaagacccgc
      961 gtacgtcgaa actcgtaaat ttacaatcat aaatccaagg agcatctatg tccctcattc
     1021 aagaaaagac cgaccaagcc gttcaaattc tcaaagagca agatattgac ctctggctca
     1081 ccttcgtccg cgagacaagc ggagtccgcg acccggtgtt gagtttcatc atcggtgaag
     1141 ctgacctgac ttggccctcc gcgctgatta tgacaaaaac cagagacaaa attgcgattg
     1201 tcggcaatct tgaaaccgaa gcattgcagc gcttggacgt gttcactcag atcattgggt
     1261 acgatacggg cgtcagcggt gtgctgcgtg agacaatttc aaaactcaac ccgaatcaaa
     1321 tcgcggtcaa tatgtccaga aacaacgtcc atgccgatgg gttgacacat gccatgtatg
     1381 aatttctggt ggaatatctc tcaggcacac cataccccga acgcattatc agcgcggagc
     1441 cggtcatcaa cgccctgcgt ggacgcaaga ccaaagccga acttgcccgc attcaaagag
     1501 ccgtagaaat cacagatgaa atctacaaga agacctttgc ttttatcaaa gttggcatga
     1561 ccgaaattca gatcgcagat tacatgcaag ccttgatgaa agaatatggc gttggctatg
     1621 cctggccccg cgagaataac ccggcggtca attcaggacc cgataaagtg gttgggcata
     1681 acggaccgac cgatatcaag gttgagccgg ggcatatcat ccacttcgac tttggcgtga
     1741 aatacgaagg ctattgctcc gacattcaac gcattgctta tgtgttacgc gaaggcgaaa
     1801 ctgaagcacc agtagaagtt cagcgcgggt ttatcaccgt ccgcacggcg atagagaaat
     1861 cgcgtgaggc gatgaaagct ggagtcat
//
LOCUS       DAOQNJ010000548         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_53840_length_1886_cov_8.445112, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000548 DAOQNJ010000000
VERSION     DAOQNJ010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53840_length_1886_cov_8.445112"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PLG52_12770"
     CDS             complement(<1..1246)
                     /locus_tag="PLG52_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB42370.1"
                     /translation="MKLKLFTMLVILGLVLSACSSGGNAQATESTSGGGGGGTVTLII
                     PEEPTTLNFYLSDAAIVRQVADSTSMTGLVTIDESGNYVPMLADGLPTISDDHLTVTW
                     KLKDGLKWSDGEALTSDDVRFTVEVLSNPDSGALVGTSGFDMISSVETPDDLTTVLTY
                     SEPYPGYLDQFAYGLFPRHATGAPEEMSSWDWNRAPVTAGPFILSNWESGVSITLTRN
                     PYYFEEGKPYLDSIVFAIVPEPAAQTAMMMNGEAQVHLWPGEFKADYDSMLADKAQQY
                     LIPGIWNMAIDFNLSAPFDGDPSAAAPHPILGDIRVREAIASAINYQALQQDVLQGSV
                     GDSTNPFAYGWYECKLPRKYGYDVEAANKLLDEAGWVMGDDGIRVAKGAMYAKDGTRL
                     SLELQGYTAFDPLQLTEEYIVEN"
     gene            complement(1273..>1886)
                     /locus_tag="PLG52_12775"
     CDS             complement(1273..>1886)
                     /locus_tag="PLG52_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB42371.1"
                     /translation="LLSISLGVVMGALSGYFGGLLDSTLMRITDAFLTFPTIFVLIIL
                     GAFLREQQLYILQNSIFVVIIIIAALSWMWPARLVRGLFLVLREKEFVTASRAMGGSD
                     LRIIVRHILPNCVGPILVSGTLQMASAIITESGLSYLGFGVQPPTPTWGSILSTAQNQ
                     VFRAPWLAFYPGLMIFVTVMAINYIGDGLRDAFDPYVIHTKSQ"
BASE COUNT          507 a          478 c          466 g          435 t
ORIGIN      
        1 ggttttcgac gatatattct tcagtgagct gcaaaggatc gaaagcggta tacccctgaa
       61 gttccagaga gaggcgggtt ccatctttgg catacatggc accttttgcc acgcggatgc
      121 catcatcgcc catcacccaa cccgcttcat ccaataattt attggctgct tccacatcat
      181 acccatactt gcgcggaagt ttacattcat accaaccata ggcgaatgga ttggtcgaat
      241 caccgacact gccctgcaat acatcctgct gcaaggcttg gtaattgatg gcgcttgcaa
      301 tcgcttcgcg gacgcgaata tcacccaaga tggggtgtgg agccgctgca ctcggatcgc
      361 catcgaacgg tgcgctcaag ttgaaatcga tcgccatatt ccaaatgcca ggaatgagat
      421 attgctgagc tttgtcagcc agcatgctgt catagtctgc tttgaattcg ccgggccaca
      481 aatgaacttg ggcttcaccg ttcatcatca tagcagtttg cgcagcaggt tcaggcacaa
      541 ttgcaaacac gatgctatcg agatagggtt ttccttcttc aaaataataa gggttgcgcg
      601 tgagggtaat gctcacgcct gattcccaat tgctcaaaat gaaaggacct gctgtaactg
      661 gcgcgcgatt ccaatcccag ctcgacattt cttcaggtgc acccgtggcg tggcggggga
      721 acaatccgta agcgaattga tcgagataac ccgggtaagg ttctgaatag gttaaaaccg
      781 tggtcaagtc atctggcgtt tcgacacttg agatcatgtc aaacccgctg gtgccaacca
      841 acgccccaga atcgggattg gataacactt caacggtaaa gcggacatca tctgaggtga
      901 gtgcttcacc atcagaccat ttcaaaccat ctttcaactt ccaagtaaca gtgaggtggt
      961 catctgaaat cgtgggtagc ccatctgcta acatgggcac ataattccca gattcgtcga
     1021 tggtcactaa cccggtcatc gaagttgaat ctgcaacctg tcggacaatc gcagcatctg
     1081 atagatagaa gttcagcgtg gtgggctctt ctggaatgat aagggtgacg gtgccgcctc
     1141 cacctccgcc gctagtggat tctgttgctt gggcatttcc gccgctggaa caagcactca
     1201 gaaccagacc caaaataaca agcattgtaa acagtttgag tttcatctct tctccttttt
     1261 gatttataaa ctctattgcg atttggtatg gatcacatac gggtcgaacg catctcttaa
     1321 cccgtcgcca atatagttga tcgccatcac ggtgacgaag atcatcaagc ctgggtaaaa
     1381 cgccagccac ggcgcacgga agacttgatt ttgtgcagtg gataaaatgc tgccccatgt
     1441 aggcgtaggg ggctgcacac caaaccctaa ataactcaag ccagactcgg tgatgattgc
     1501 cgaagccatt tgcaaagttc cgctcaccaa gatcggaccg acacaattcg gcaaaatatg
     1561 gcgaacaata atgcgcaaat cgctgccacc catggcacgc gaggcggtga caaattcttt
     1621 ttcacgcaac acgaggaaca gtccgcgtac gaggcgggcg ggccacatcc acgagagggc
     1681 ggcgataatg atgatgacaa cgaagatgct gttttgcaaa atgtacaatt gctgttcgcg
     1741 caaaaaggcg ccaaggatga tgagtacaaa gatggtgggg aaggttagga aggcatctgt
     1801 gatacgcatc agggtcgagt cgagcaggcc gccaaagtag ccacttaatg cacccatgac
     1861 aacccctaac gaaatggata acaaag
//
LOCUS       DAOQNJ010000549         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_54286_length_1877_cov_9.231065, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000549 DAOQNJ010000000
VERSION     DAOQNJ010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54286_length_1877_cov_9.231065"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLG52_12780"
     CDS             <1..231
                     /locus_tag="PLG52_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42372.1"
                     /translation="AQGSADAAVIAAKGEAEALLIQAQAQAEANQVIAQSLTPELVQY
                     QYVQKLSPNVQTIFIPSGNQFILPLPSSPQGQ"
     gene            289..1347
                     /gene="selD"
                     /locus_tag="PLG52_12785"
     CDS             289..1347
                     /gene="selD"
                     /locus_tag="PLG52_12785"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:349..351,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNB42373.1"
                     /translation="MSIPQSGREVRLTTLSACAGUASKLSADALAQVLRPVKDIFDPA
                     AYPDLMVGLESPDDAAIWRLSDDQAIVVTTDFFTPVVDTPYEYGAIAAANSLSDVYAM
                     GGRPFLALNIAALPDHLPAEISGEILRGGAEKAREAGVVIAGGHTVKDKEPKYGLVVI
                     GFVHPQKVISKGGLKAGDVLVLTKPLGAGVTTTALKQEKADEAHVLEAIEWMARLNKN
                     AGMLASEFGVRGGTDITGFGFLGHASEMAEASHVALHFDYSKIPFLSGARGYAERGIF
                     PGGAFDNKSHFEALVKFSAEISEPAQMLLFDPQTSGGLLFGLAKEKLSAFEARAKEIN
                     QPYWVIGEAREGEGIEVG"
     gene            1349..1495
                     /locus_tag="PLG52_12790"
     CDS             1349..1495
                     /locus_tag="PLG52_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42374.1"
                     /translation="MFRVGVLELGVTCAFVALTIIIPLLIARGYAQINKRLKNIENTI
                     AKKK"
     gene            1687..>1877
                     /locus_tag="PLG52_12795"
     CDS             1687..>1877
                     /locus_tag="PLG52_12795"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNB42375.1"
                     /translation="MSISSEIITIIILIVINGVLAMSEAALVASRKARLQQQASEGNK
                     ASALAVKLIEDPNIFLSAV"
BASE COUNT          479 a          417 c          496 g          485 t
ORIGIN      
        1 gcgcaaggtt ctgcagatgc cgccgtgatc gcagcgaaag gtgaggctga ggcgttgttg
       61 attcaggctc aggcgcaggc agaggcgaac caagtcatcg ctcaatcact cacgccggaa
      121 ctggttcaat atcagtacgt gcagaagctt tcgccgaacg ttcagacgat cttcatccca
      181 agcggcaatc aatttattct gccgttgccc agttcacccc aagggcagta atcgagtaca
      241 ctacagacct gacggttccc gtcaggtctg taatctataa aggagcatat gtccattcca
      301 caaagcggac gtgaggtgcg cctcaccact ctttcagctt gtgctggttg agcgtctaaa
      361 ctaagcgctg atgcgctggc gcaggttctg cgccctgtca aagatatctt tgaccctgct
      421 gcatatcccg acttgatggt gggtcttgag tcgccggatg atgcggcaat ttggcgttta
      481 agtgacgatc aagcgattgt ggtaacgaca gatttcttca ctcccgttgt agacactcct
      541 tatgaatatg gtgcaattgc tgcagcaaac agcctctcag atgtctatgc catgggcgga
      601 cgtccgttct tggcgttgaa tattgcggca ttgccagatc acctaccggc ggagatttca
      661 ggcgaaattt tgcgtggtgg tgccgagaaa gcccgtgaag cgggagtcgt tattgcaggt
      721 gggcatacgg tcaaagacaa agagccaaaa tacggcttgg tggtgatcgg gtttgtgcat
      781 ccacaaaaag tgattagcaa gggcggatta aaagccggcg atgtacttgt gcttaccaaa
      841 ccgctcggcg ctggagtcac cacgacggcg ctaaaacaag aaaaagctga tgaggcgcat
      901 gttctcgaag ccattgaatg gatggcgcgt ttgaacaaaa atgcggggat gcttgcttct
      961 gaatttggcg tccgcggtgg gaccgatatc actggcttcg gttttctggg tcacgcctca
     1021 gagatggcag aagcatctca tgtggcattg cattttgatt attcgaaaat cccatttttg
     1081 agtggcgcac gtggttatgc tgagcgtgga attttccctg gtggagcgtt tgataacaaa
     1141 agtcattttg aagctctggt gaagttctct gctgaaataa gtgaacctgc tcaaatgctt
     1201 ttgttcgacc cgcaaaccag cggcggatta ttgtttggtt tggcaaaaga aaaactgtcc
     1261 gcttttgaag cgcgggcaaa ggaaataaac caaccgtatt gggtaattgg agaagcacgc
     1321 gaaggcgaag gcatcgaggt cggttagtat gttccgagtt ggcgtgctgg agttgggtgt
     1381 cacttgtgca tttgtggcgt tgacaatcat tattccgtta ttgattgccc gtggatatgc
     1441 acaaatcaac aaacgtttga agaatattga aaatactatc gcaaaaaaga agtaactttc
     1501 aggtacaata agggcgactt acttagcacg aaacatcatg tccgacattc ccatagaacc
     1561 agaccgtagt tccccctgtc cgtttcaatt agcttggtgg aaatctccac gccgcgaagt
     1621 tctttatgcg cggcttgtat ctttttaagt agtatcaaac gaaattctag agtcccatag
     1681 gaagcaatga gcattagttc tgaaatcatt accatcataa tcttgatcgt catcaatggc
     1741 gttttagcaa tgtctgaagc ggcattggtt gcctcacgca aggcgcgctt gcaacaacag
     1801 gcaagtgaag gcaataaagc ttcggcattg gctgttaagt taatagaaga ccccaatata
     1861 tttttatcgg cagtgca
//
LOCUS       DAOQNJ010000550         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_54772_length_1868_cov_8.970215, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000550 DAOQNJ010000000
VERSION     DAOQNJ010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54772_length_1868_cov_8.970215"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..>1868)
                     /locus_tag="PLG52_12800"
     CDS             complement(207..>1868)
                     /locus_tag="PLG52_12800"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB42376.1"
                     /translation="SLFLDVGQEKVDEFRMKRKDGSEIWVEDHGRYIHDENGNVIYHE
                     GILRDITERVYASEAARKAEEKYRSIFENAVEGIYQSSPDGKFHTVNPAFAAMLGYTS
                     PQEMMLSIKDIANQVYSDAYRRQDFTKQIEEKGEVNGFEYQMRRKDGRLIWVSENARL
                     VRDTKGEILYYEGILEDITARKQNEQRIDNQLKQLNALHAIDNAINSNFDLRTTLDVI
                     LREVTAQLRADATSVLLFNKETHTLDYTAGRGFISKATQHTKLSIGEGYAGRVILDRK
                     MIHIPNLTQTENYRLAEALNLAGENITAYVGSPLIAKGQVVGVLEIFQRAPLAPDPDW
                     FDFLSMISDQAAIAIDNAQLFENLQRSNFDLTLAYDATIEGWSRALDLRDRETEGHTQ
                     RVTNLTVKLARQMGVPDADILHIRRGALLHDIGKMGIPDKILHKPDKLTAEEWAVMRQ
                     HTNYAYQMLTPITYLKPALDIPRCHHEKWDGTGYPDGLKGEQIPIAARIFAVVDVWDA
                     LTSDRSYRDAWTKEKAIEFIRSESGKHFDPKVAETFLSFILKETP"
BASE COUNT          423 a          408 c          499 g          538 t
ORIGIN      
        1 aagctgtaga agctttgatc gaaggtacac atggcgtgat gacgggactg aagggtaaag
       61 gcattgactt tatccccttg ggcgatgtca ttagcaataa gcgcaaggtc aacatggagt
      121 attaccacat ggtgaaggtg ttggcgcgct aaataaagca gcgtagataa aaagacggtg
      181 gatgatttca tccgccgtct tttggtttaa ggtgtttctt tcaaaatgaa agacaggaag
      241 gtttctgcga ctttgggatc aaaatgcttc cccgactctg atcggatgaa ttcgatagct
      301 ttttcttttg tccacgcatc acgataggac ctgtcactgg tcagcgcatc ccaaacatct
      361 actacggcga agattcgtgc tgcaatgggt atctgctcgc cttttagtcc gtcggggtag
      421 ccggtgccgt cccatttttc gtggtggcaa cgtggaatat ccagagctgg ttttagatag
      481 gtgatgggtg tcaacatttg ataggcatag ttagtgtgtt gacgcataac tgcccattct
      541 tcggctgtaa gtttatctgg cttgtgcaag attttatcgg gtatgcccat tttgccaatg
      601 tcgtggagca aggcaccacg acgaatgtgc aaaatatcgg catcgggaac gcccatttga
      661 cgtgccagtt tcacggtcag gttggtgacg cgttgggtgt gtccttcggt ctcgcgatcg
      721 cgtaggtcta aggcgcgaga ccagccttca atggtggcat cgtatgcaag tgtgaggtcg
      781 aagtttgagc gttgtagatt ttcaaagagc tgagcgttgt ctatggcgat agcggcttgg
      841 tctgagatca tgctcaggaa gtcgaaccaa tctgggtcgg gggcgagagg cgcacgttga
      901 aagatctcca gcacgccgac aacttgtccc ttagcgatga gcggagaacc cacataggcg
      961 gtgatatttt ctccggcgag gtttaacgct tcggcgagac ggtaattttc agtttgggtc
     1021 aagtttggaa tgtggatcat cttccggtct agaatgacgc gcccggcata tccttcgcca
     1081 atactgagct tggtatgttg ggtggcttta gagatgaaac cgcgcccggc tgtgtaatct
     1141 aaggtgtggg tttctttatt gaacaacaat acagaggtgg cgtctgctcg caactgtgct
     1201 gtcacttctc gcaagatgac gtcgagggtg gtgcgcagat cgaagttgga attgatggca
     1261 ttgtcaatgg cgtgcaatgc gttcaattgt tttaattgat tgtcaatgcg ctgttcattt
     1321 tgtttgcgag cggtaatatc ttcgagaatg ccttcgtaat atagaatctc accctttgta
     1381 tcgcgcacga gccgggcatt ttctgagacc cagatcagcc ggccatcttt gcggcgcatt
     1441 tgatattcga agccatttac ctcgcctttt tcttcgattt gtttcgtaaa atcttggcga
     1501 cgataggcat cgctatagac ctgattggca atgtctttaa tggatagcat catttcctgc
     1561 ggcgaggtgt atcctagcat ggcagcaaat gcggggttta cggtgtggaa tttcccatcg
     1621 ggcgaacttt ggtaaatacc ttccacggca ttttcaaaaa tgctgcggta tttttcttcc
     1681 gctttgcgtg cggcttctga agcatagaca cgttctgtaa tatcgcgcaa gatgccttca
     1741 tgatagataa cattcccatt ttcatcatga atatagcgtc cgtggtcttc cacccagatc
     1801 tcagacccat ctttgcgctt catacggaac tcatctactt tttcctgccc aacatcaagg
     1861 aagaggct
//
LOCUS       DAOQNJ010000551         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_54775_length_1868_cov_5.748483, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000551 DAOQNJ010000000
VERSION     DAOQNJ010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54775_length_1868_cov_5.748483"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLG52_12805"
     CDS             complement(<1..384)
                     /locus_tag="PLG52_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42377.1"
                     /translation="MDDLDLLKKYEPVLRFAKSERFFPMAVEPYLEKCTLFPSGPSGA
                     AELFGHLNEPLIGKIGNLESHEFFLRFVNKPLNDFDAWIWWGVGSVLALVAGWFTLGV
                     AGVESFIALSLSTALVLFMLASPIRL"
     gene            411..1553
                     /gene="galK"
                     /locus_tag="PLG52_12810"
     CDS             411..1553
                     /gene="galK"
                     /locus_tag="PLG52_12810"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNB42378.1"
                     /translation="MDPKILSQHFQTHFNAKPEIIVRAPGRVNLIGEHTDYNDGFVLP
                     MAIDREVMIALRPREDETVRLFSLDLETESAFELSSLSREGDWIEYPKGIAYELLKAG
                     FKIKGFDAIMTGDVPRGAGLSSSAAVELAVARAFSAVSGFEWNAPAMAKLAQKAENEW
                     VGVNCGIMDQMASAACKEGFALFLDCRSLEIQHAPLPKGVSVVILDTSTRRGLVDSAY
                     NERRSQCEEAARWFGVKALRDVSTQEIGKWNPGSELSEIAFRRARHIVTENERVLEAI
                     DIMRKGNVKRLGELFNASHDSLRDDFEVTNDALNIVVECAREQSSCYGARMTGAGFGG
                     CAVALVAEEKTTEFVKAVSAAYKQRSSLEASIYVCKASEGASILQG"
     gene            complement(1594..>1868)
                     /locus_tag="PLG52_12815"
     CDS             complement(1594..>1868)
                     /locus_tag="PLG52_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42379.1"
                     /translation="GYARCDYRMDADGTIHMLEINPNCGIFYPPHEPGSADFSLLNDP
                     NTDHTKFMKLIISNAQHRQTRIAAERIIKRQQRKRERVPVEQMAYT"
BASE COUNT          496 a          435 c          479 g          458 t
ORIGIN      
        1 caaacgtatc ggcgatgcca acatgaacaa caccaatgcc gttgaaagcg ataaggcaat
       61 aaagctctcc acaccagcca cacccaatgt aaaccacccc gcgaccaacg caagcacaga
      121 accaactccc caccagatcc atgcatcaaa gtcgttcaaa ggtttgttta caaagcgtag
      181 aaaaaattca tggctttcca agttaccgat cttcccgatc aatggttcat tcaaatgacc
      241 gaataactct gccgccccgc ttggtccgct tggaaagagc gtacacttct ccaaataagg
      301 ctctacagcc atggggaaga aacgctcact cttcgcgaag cgcaaaacag gttcatactt
      361 ttttagtaaa tcaaggtcat ccatgaagcc tctatggtat aaacgcagat atggacccga
      421 aaattctctc tcaacatttc caaacccatt ttaacgccaa accggagatc atcgtccgcg
      481 cacctgggcg ggtcaacttg attggcgaac atactgacta taacgacggc ttcgtgctac
      541 ccatggcaat tgaccgtgaa gtgatgatcg cactccgccc gcgtgaggat gaaaccgtcc
      601 gcctcttttc tctggacctt gaaactgaat cagccttcga gttatcgtct ctcagccgag
      661 aaggtgactg gatcgaatat cccaaaggta ttgcctatga attactgaaa gctggtttca
      721 agataaaagg cttcgatgcc atcatgaccg gtgatgtacc acgcggtgct ggactttctt
      781 catccgctgc tgtggagttg gcggttgccc gtgctttttc tgccgtctct ggttttgaat
      841 ggaatgcgcc cgccatggca aagcttgccc aaaaagcaga gaatgaatgg gtgggtgtca
      901 actgcggcat tatggaccag atggcaagcg ctgcatgcaa agaaggcttt gccctgtttt
      961 tagattgccg ttctttggaa attcaacatg cccctctacc aaaaggagtc tctgtcgtta
     1021 tccttgatac atctacccgg cgcgggttgg tagattccgc gtataacgag cggagaagtc
     1081 aatgtgaaga ggcggctcgc tggttcgggg taaaagcgtt gagagatgta agcacacaag
     1141 agattggaaa gtggaacccc ggaagcgagt taagtgaaat tgcctttagg cgtgcccgtc
     1201 acattgtcac tgagaatgag cgcgtgcttg aagccattga cataatgaga aaagggaatg
     1261 taaaacgtct gggtgagtta ttcaacgcca gtcatgacag tctacgggat gattttgagg
     1321 tgacgaatga tgcgcttaat attgtggttg aatgcgcgag agaacagtcc agttgttatg
     1381 gcgcccgtat gactggtgca ggctttggcg gctgcgcagt tgctctggtt gcagaagaaa
     1441 aaactacaga gtttgtaaaa gccgtttcag cggcatacaa gcaaaggtca agtttggaag
     1501 cgagtatcta cgtctgcaaa gccagtgaag gagcaagcat ccttcaggga tagaaaaacg
     1561 ggacactgtg aagtgtcccg ttttttgttt tgattaggta taagccatct gctcgaccgg
     1621 cacgcgttcg cgtttgcgtt gctggcgttt gatgattctt tcagcagcaa tgcgagtttg
     1681 gcgatgttgg gcgttgctaa tgatcaactt catgaacttg gtgtgatccg tgtttgggtc
     1741 attgagcagt gagaaatcgg cagagcccgg ttcatgaggc gggtaaaaga taccgcagtt
     1801 gggattgatt tccaacatat gaatggtgcc atctgcatcc atgcgatagt cacagcgggc
     1861 ataaccat
//
LOCUS       DAOQNJ010000552         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_54829_length_1867_cov_8.546358, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000552 DAOQNJ010000000
VERSION     DAOQNJ010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54829_length_1867_cov_8.546358"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1867)
                     /locus_tag="PLG52_12820"
     CDS             complement(<1..>1867)
                     /locus_tag="PLG52_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB42380.1"
                     /translation="IGNKAIGLVFIGNYEKEHAFDESDVRLLQTVVNAMSVALENARL
                     FDETQHLLKITEDRAAELAIINSVQEGLASKLEMQAIYDLVGDKLAEVMNTLDIDIRI
                     FSPETNQVFFPYLREHGERLEISPSPMRGFAKKIHETRQTIVINENLAERMAELGSVL
                     VPGTQMEKSFMAVPILVGGAALGMVSISNYEKENAFGDSDIRLLQTVVSAMSVALENA
                     RLFDETQRLLKITEDRATELSVINSIQQGLAAELDPQAIIELTGDKLRETLQTEDLGI
                     RLYDAQNNLIHYVYEYIQGERREYPSKPPVPGGTFERIFKTRQPFIRNTLAEREALGL
                     ALLPNTEAAKSSVNVPIIGSDRVIGVIIVQSHEKEYAFSDAQVRLLQTVAASMGVSLE
                     NGRLFDETQRLLKETEQRNAELAIINAVQQALVSNLDMKSIYQAVGRKLTEIFSVQSA
                     AIYTVDFNTKVMTYEFAYEQGKEWEIEPKPATGLHLNVINQVLSSRKSFVVNTNFNEY
                     ASQFADFQSSRSRNPKSLCAVPILIRENSITGISLQHLEKENFFDESALRLLETISSA
                     AGVALENARLFDETQRLLQETEERAAELGAISTVTQALVAETELDNMIQLIGSQMR"
BASE COUNT          408 a          452 c          509 g          498 t
ORIGIN      
        1 cgcatctgac tcccgatgag ctggatcatg ttatccagtt ctgtctctgc caccaaagcc
       61 tgagtgacgg tactgatggc gcccagctca gcggcgcgtt cttcggtctc ctgtaagagg
      121 cgctgggttt catcaaagag acgggcattt tcgagcgcaa ccccagccgc actggagatc
      181 gtctctagca atctcagcgc agactcgtcg aagaagttct ctttttcaag atgttgcagt
      241 gagatgcctg taatcgaatt ctcgcggatc aagatcggca ccgcacaaag tgacttggga
      301 ttgcgtgagc gcgatgactg gaaatcagcg aactgcgagg catactcatt gaagttggta
      361 ttcaccacaa agctcttgcg agaggaaagc acttggttga tgacattcaa atgcagaccc
      421 gtagctggtt tgggttcgat ctcccattct ttgccttgtt cataggcgaa ctcataggtc
      481 atgacctttg tgttgaaatc aacagtatag atcgccgcag attggacact gaagatttcg
      541 gttaactttc tgcccactgc ctgataaatg gacttcatgt ccagatttga gaccaaggct
      601 tgttgaacgg cattgatgat tgccaactcg gcattgcgtt gttcggtttc tttcagcaag
      661 cgttgagttt cgtcgaagag acgtccgttc tcaagcgaga cacccatgct ggcggcaacg
      721 gtctgcaaca aacgcacctg cgcatcgctg aaggcgtatt ctttttcgtg gctttgtacg
      781 atgatgacac caatgacacg gtcactaccg atgatgggca cgttgacgct ggatttggcg
      841 gcttcggtat ttggcaacaa ggcaagtccg agtgcttcac gttcagccag agtattgcgt
      901 ataaagggtt ggcgggtttt gaagattctc tcgaaggtac caccaggcac tggtggtttg
      961 gatgggtact cgcggcgttc accctgaatg tattcgtaca cgtaatgaat gagattgttt
     1021 tgggcgtcgt acaagcgaat gccgaggtct tcggtttgaa gtgtttcgcg cagtttgtct
     1081 ccggtcaact cgataatggc ttgcgggtct aattcggctg ccaagccctg ttgaatgctg
     1141 ttgatcacgg atagttctgt ggcgcggtct tcggtgatct ttaagaggcg ctgggtttca
     1201 tcgaagagac gcgcattttc gagcgccacg ctcatggcag agaccacggt ttggagcagg
     1261 cgtatatcag aatcgccgaa ggcattttct ttttcgtaat tgctgatgct gaccatgccc
     1321 aatgccgcgc cgcccaccag aatgggcacc gccatgaagg acttttccat ttgcgtgccg
     1381 ggcaccagca ccgagccgag ctctgccatg cgttctgcca ggttttcgtt gatcacgatg
     1441 gtctgacggg tttcatgaat ctttttagca aacccgcgca tgggcgaggg ggagatttcc
     1501 agccgttccc catgttcacg caggtagggg aaaaagactt gattcgtctc tggcgaaaag
     1561 atgcggatgt cgatatcgag ggtattcatc acctcggcta atttatcgcc gaccagatcg
     1621 taaatggctt gcatctccag tttagaggcg agaccttcct gcaccgagtt gatgatggcg
     1681 agttcggcgg cgcggtcttc ggtgattttt aagagatgtt gggtttcatc gaacagacgg
     1741 gcattttcga gcgccacact catggcgttg accacggttt gtaacaggcg cacgtcactt
     1801 tcatcgaagg catgttcttt ctcgtaattg ccaatgaaaa ccaagccaat ggctttattt
     1861 cctatca
//
LOCUS       DAOQNJ010000553         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_54870_length_1866_cov_7.799006, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000553 DAOQNJ010000000
VERSION     DAOQNJ010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54870_length_1866_cov_7.799006"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PLG52_12825"
     CDS             <1..898
                     /locus_tag="PLG52_12825"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNB42381.1"
                     /translation="EMLGKEAALFVPSGTMGNLLALLVHCSRGEEVICGDKSHIYVNE
                     AGGMAALGGIYPHPVPNQKDGTLRLEDIRASIQPDDSHRTITRLVCIENTQNMCGGVV
                     LSVEYTKQVGQLAKEYRLKFHIDGARIFNAAAALNVDIKELVAPADSVMFCLSKGLVA
                     PIGSMLVGSKEFIAKAHRLRKMLGGGMRQVGVMAAAGLVSLDKMTGRLKQDHARAKNL
                     YEGLKQVKGLRLEAEPVSNMVYFSLADHITLTENQICAEMKKHGVMVDWSAPRQFRLV
                     THYWVDDAGVEKTVKALGELLK"
     gene            complement(899..>1866)
                     /locus_tag="PLG52_12830"
     CDS             complement(899..>1866)
                     /locus_tag="PLG52_12830"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNB42382.1"
                     /translation="LAKNLVTGIVLFNTTGEVIAEVLVNDDGKTVNPEHPMSLIQDAM
                     KSGQMIYVSPDESTIRTCLPIQSPIRGYGAIWMDTVDDPSKPSTNPNDLQALVNQSAI
                     ALERSLLLVESRRQAKEIKEAYDILEVTYDQTLASLTAALDARDRETEGHSLRVTKLA
                     VRLGETLGYKAKQLKILERGSLLHDIGKIGISDTILHKPGPLTEAEWEIMRLHPDIGA
                     KIVEGIPFLEETIPLIRHHQERWDGSGYPAKLKGTDIPELARLFSVIDAFDALTSNRP
                     YRQKISIKEALEYLKEMSGIHFDPDMVINFEALINQNPEIVQSGD"
BASE COUNT          442 a          384 c          525 g          515 t
ORIGIN      
        1 agagatgctt ggcaaggagg cggcgttgtt cgtcccttcg ggcacgatgg gcaatttgtt
       61 ggcattgctt gtacattgtt cgcgcggcga agaagtgatc tgcggcgaca agtcgcatat
      121 ttatgtcaac gaagctggtg ggatggctgc gttgggcggg atttatcctc atcccgtgcc
      181 gaatcaaaag gatggaacac ttcggcttga ggacatccgc gcctcgattc aacccgatga
      241 ctcacatcgc accattacgc gtttggtttg cattgagaac acgcagaaca tgtgcggggg
      301 tgttgtcctt tctgtggagt acacaaagca ggttggtcaa ctcgcaaaag agtatagatt
      361 gaaattccat attgatggtg cgcgtatttt caacgcggcg gctgctttga atgtggatat
      421 caaagaactt gttgccccag ccgattcggt gatgttctgt ttgagcaagg gcttggtcgc
      481 gccgattggg tcgatgttgg ttggctcaaa ggaatttatt gccaaggcgc accgtctgcg
      541 taagatgtta ggtgggggta tgcggcaggt tggggtgatg gcggcggcag gattagtgtc
      601 gctggataag atgacggggc ggcttaagca ggatcatgcc cgggcgaaga atctgtatga
      661 aggcttgaag caggtgaagg gattaagact cgaggcggag cctgtatcca atatggtgta
      721 tttcagtttg gcagatcata ttacgctgac cgaaaatcaa atctgcgctg aaatgaaaaa
      781 acacggcgtg atggtggatt ggtctgcgcc acgacaattt cgcttggtga cgcattattg
      841 ggtggatgat gcaggtgtgg aaaaaaccgt gaaagccctt ggcgaattgc tgaaataatc
      901 aatcacctga ttgaacaatt tcagggtttt gattgatgag cgcttcgaaa tttatgacca
      961 tgtctgggtc aaaatgaatg ccagacattt ctttcaaata ttcaagtgct tctttgattg
     1021 atattttctg acgatagggg cgattgcttg tcagtgcatc aaaggcatcg atgacagaga
     1081 aaaggcgtgc taactctgga atatcggtgc cttttagttt ggcagggtag ccgctaccgt
     1141 cccaacgttc ttggtgatgg cggataagtg gaatggtctc ttcaaggaag ggaatgcctt
     1201 cgacgatctt tgcacctata tctgggtgca gacgcataat ttcccattct gcttctgtca
     1261 gcggacctgg tttatgtaag atagtatcgc tgatgccgat ttttccaata tcatgcaaaa
     1321 gagaaccacg ctcgagaatt ttgagttgct tggctttata ccctaaggtt tctcccaacc
     1381 gtacggcaag cttggtcaca cgcaaactgt gaccttctgt ctcgcggtct cgggcatcta
     1441 atgcagcagt aagagaagcg agcgtttggt cgtaggtaac ttcgaggatg tcgtacgctt
     1501 cttttatttc ttttgcttgc cggcgcgact cgaccagaag aagtgagcgt tccaaggcta
     1561 tggcagattg attaacaagg gcttgcaggt catttgggtt ggtcgatggt ttggatgggt
     1621 cgtccactgt atccatccaa atggcgccgt atccgcgaat gggagattga atgggcaagc
     1681 aagtacggat cgtcgattca tctggcgaga cataaatcat ctgacccgat ttcattgcat
     1741 cttgaattaa actcatgggg tgttctgggt tgacagtctt gccatcgtca ttgaccaaca
     1801 cctctgcaat aacctctcct gttgtattga agagcacgat acccgtcacc aagtttttgg
     1861 ctaatt
//
LOCUS       DAOQNJ010000554         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_54937_length_1865_cov_9.344751, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000554 DAOQNJ010000000
VERSION     DAOQNJ010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54937_length_1865_cov_9.344751"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLG52_12835"
     CDS             <1..732
                     /locus_tag="PLG52_12835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB42383.1"
                     /translation="SGIGKATALRFAEEGADLVVTGRRVSLGKVVEAECRQKGVRCVF
                     VEADHSKVEDCQRVVETALKEFNRIDILFNNAGIVTKGTAETTSDETWQDTLNINVTA
                     VWKMCQLVLPQMKKQGKGAIVNNGSDWSVVAGKNAFPYIMSKGAVGMMTKAMALDYAR
                     DGIRVNAVCPGDTFVDRWMEKGYFEGSDPVKIEDAIQEASEYLPMGRFGKPEEIANAV
                     VFLASDEASFVTGHLLLVDGGNTAQ"
     gene            785..1444
                     /locus_tag="PLG52_12840"
     CDS             785..1444
                     /locus_tag="PLG52_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNB42384.1"
                     /translation="MIVVSDMMGTLTTGSPFRGLVDWVKHNQSKARANWYLASIAPSY
                     FLAKKGVLDWQDWGHRLMINSLSYIKDATPENVAHAAEWVVEKDLWVKRREDVIARLT
                     KHREEGASIYIASSYFEPFLEPFAKRIGAKGIGTPVELVDGRLRMTSKQMVADERKLE
                     QVLTRLGVERVDVAYGDTILDVPLLEHANHPVAVYPDVKLKKIALERGWEIIGDTPTY
                     E"
     gene            1483..>1865
                     /locus_tag="PLG52_12845"
     CDS             1483..>1865
                     /locus_tag="PLG52_12845"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNB42385.1"
                     /translation="MERTTIRRSRKRRADSKIYLCAPVNSLVEGIYEQNIPFHEIRKH
                     GDMGLGTFNDLDGEMMMVDGEIYRVGSDGKVNLITDDDTCTPFACVTFFQPASDEVLE
                     KEMDYEQFLRWMMELMPSPNIFYAFR"
BASE COUNT          488 a          349 c          548 g          480 t
ORIGIN      
        1 tcgggtattg gcaaggcgac ggcgttgcgg tttgccgaag aaggcgcgga tttggtggtc
       61 acagggcgac gtgtcagctt ggggaaggtt gtcgaagcag agtgcaggca aaaaggagtc
      121 cgatgcgtgt ttgtggaggc agatcactcc aaggtagaag attgtcagag agttgtcgaa
      181 actgcactta aggaatttaa tcgcattgat attttgttta ataatgctgg gattgtgacg
      241 aaggggacag cggagacgac cagtgatgag acttggcaag acacgttgaa tatcaatgtg
      301 acggcggttt ggaagatgtg tcagttggtg ttgccgcaga tgaagaagca aggtaaaggt
      361 gcgattgtca ataatgggtc agattggtct gtggtggcag ggaagaatgc ctttccgtat
      421 atcatgagca aaggcgcggt gggaatgatg accaaggcaa tggcactcga ctacgcacgt
      481 gatgggattc gtgtgaacgc ggtttgccca ggggatacct ttgtagatcg ctggatggag
      541 aaaggttact ttgaagggtc agaccctgtg aaaattgaag atgctattca agaagccagt
      601 gagtatttgc ctatgggtcg ttttgggaaa ccagaagaga ttgctaatgc ggttgtgttc
      661 cttgcttctg atgaagcgtc ttttgtgacg ggacatttgc tcttggtcga tggtgggaat
      721 acagcgcagt gatatactgc aaaagttttt aagtatctag ttgaaagttt tagaaggagt
      781 ttaaatgatt gttgtttccg atatgatggg caccttaacc acgggttccc cttttcgcgg
      841 gttggtggat tgggtaaagc ataaccaaag taaagctcgt gccaactggt atctggcgtc
      901 gattgcacct tcgtattttc tggcaaagaa aggagtactt gattggcagg attgggggca
      961 tcgtttgatg atcaacagct tgtcatatat taaggatgcc acccccgaaa acgtagctca
     1021 tgccgccgaa tgggttgtgg agaaagattt gtgggtcaag cgtcgtgaag atgtgatcgc
     1081 ccgtttgacg aagcatcgcg aagaaggtgc atccatttat attgccagca gttactttga
     1141 accctttctt gagcccttcg ccaagcgcat tggcgcaaaa gggatcggca cacctgtgga
     1201 acttgtcgat ggacgattac gcatgaccag caagcaaatg gtggcggatg aacgcaaact
     1261 ggaacaggta cttacccgcc tcggcgtgga gcgagtggat gtggcgtatg gcgacaccat
     1321 tttagatgtc ccattgttgg aacatgccaa tcaccctgtg gcggtgtacc ccgatgtaaa
     1381 attgaagaag atcgcacttg aacgcggttg ggaaattatc ggcgatacgc caacatacga
     1441 atgatcttgt acggaacagt ttacagagga gaatacattc aaatggaacg aacaacaata
     1501 cgcaggtcta ggaagaggcg cgcagatagc aaaatctatt tatgtgcgcc ggtcaattca
     1561 ctcgtcgaag gcatttatga acagaacatc cctttccatg aaattcgcaa acatggcgac
     1621 atgggcttgg gaacgttcaa tgacctcgat ggcgagatga tgatggtgga cggcgaaatt
     1681 tatcgtgtgg ggtctgatgg caaggtcaat ctcattacag atgatgatac ttgtacgcca
     1741 tttgcctgtg tgaccttctt tcaacccgct agtgacgaag tgctcgaaaa agaaatggat
     1801 tacgagcagt tcctgcgttg gatgatggag ttgatgccat cgccgaatat tttttatgcc
     1861 tttcg
//
LOCUS       DAOQNJ010000555         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_54941_length_1865_cov_5.085635, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000555 DAOQNJ010000000
VERSION     DAOQNJ010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54941_length_1865_cov_5.085635"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PLG52_12850"
     CDS             <1..600
                     /locus_tag="PLG52_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNB42386.1"
                     /translation="ITEEIKMSDYFVHESSYVDEGAEIGVGTKIWHFCHVMPHAKIGE
                     RCNIGQNVLVSSDVIIGMNVKIQNNVSLYTGVIVEDDVFLGPSMVFTNVINPRSHVSR
                     KDEYKTTLVRKGASIGANATIVCGTTLGRYCFVGAGAVVTKDVPDYALVYGTPARVRG
                     WVCQCGEQLTFDADHAVCSRCGDAYRKQDSVVSPERGES"
     gene            597..1697
                     /locus_tag="PLG52_12855"
     CDS             597..1697
                     /locus_tag="PLG52_12855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNB42387.1"
                     /translation="MISLVDLTAQYHSIKKEIDTAVLSTLESGHFILGPQVVKFEESI
                     AAYLGVDHAIGLASGTDALVIALRALNIGAGDEVVIPAYTFFATAGTVMSVGAKPVIV
                     DVDPQSYQIDVSKIEAAITPKTKAIIPVHLYGHPAEMNPILEISRKHGLKVIEDNAQG
                     FGAEYLGKKTGSFGDIGCLSFFPTKNLGAYGDGGAVVTNDPALAEQMRMLRAHGWKKK
                     YYSEMVGYNSRLDAMQAAILQAKFPYLDGWNEKRRELSKRYTEHLAPLGIVTPVEREW
                     AKHVYHLYIIRTSKRDALQAFLKEKGIASEVYYPLPPHLSVPCRKYGYKEGDFSHAEK
                     AAKETLALPLYPEMSLAQQDEVIAAVKEFINQ"
BASE COUNT          474 a          378 c          523 g          490 t
ORIGIN      
        1 attactgaag aaataaaaat gtctgattat tttgttcacg aatccagtta tgttgatgaa
       61 ggtgccgaga ttggtgtggg gacgaaaata tggcattttt gccatgtaat gccgcatgcc
      121 aagatcggtg agcgttgcaa cattggtcag aatgtgttgg tctcttctga tgtaattatt
      181 ggaatgaatg tgaagattca gaataatgtc tctctttaca ccggtgtgat agttgaagac
      241 gatgtatttc ttggaccatc gatggttttt acgaatgtga ttaatccacg cagccacgtt
      301 agtcgcaagg atgaatataa aacaacattg gtgcggaaag gcgcttcgat cggagcgaat
      361 gcgaccattg tctgcggcac gacactcgga cgatattgct ttgtcggtgc aggcgcggtc
      421 gtaacgaaag atgtgcctga ttatgcgctg gtgtatggca ccccggctcg tgtgcgcggg
      481 tgggtatgtc aatgtggtga acagttaacc tttgatgctg atcacgctgt atgctcaagg
      541 tgtggagacg cttacagaaa acaggattca gttgtcagcc cggaaagagg cgaatcatga
      601 tctcgttggt tgatctcacg gcgcaatacc attccatcaa aaaggaaatc gatacggcag
      661 ttttatccac tttggagtca ggtcatttta ttttaggtcc acaggtggtg aagttcgaag
      721 agagcatcgc tgcatacctt ggcgttgatc acgccattgg gctggcttca ggaacagatg
      781 cattggtgat tgcattgcgt gccttgaata ttggcgctgg tgatgaagtt gttatccccg
      841 cttatacatt ttttgccacg gctggcacgg ttatgtccgt tggcgcaaag ccggtgatcg
      901 tggatgtgga cccgcagagt tatcagatcg acgtgagcaa gatcgaagct gcgattacgc
      961 cgaagacgaa ggcgatcatc cctgtccatt tgtacgggca tcccgcggag atgaatccga
     1021 ttctggagat ttcccgcaaa catgggctga aagtcattga ggataacgcg cagggatttg
     1081 gcgcagaata ccttgggaag aagaccggtt cgtttggcga cataggctgt ttgagtttct
     1141 tcccgaccaa gaacctgggt gcatatggcg acggtggcgc ggtggtgacg aacgacccgg
     1201 cgcttgccga gcagatgcgc atgttacgcg cgcatggctg gaagaagaaa tattattcag
     1261 agatggttgg ctataacagc cgcctcgatg caatgcaggc agcgatcttg caagccaagt
     1321 ttccatatct ggacggatgg aacgaaaagc gccgtgagct ttcaaagcgg tataccgaac
     1381 atcttgcgcc gctggggatt gtcacacccg ttgagcgcga atgggcaaaa catgtttatc
     1441 acttgtatat catccgcact tcaaaacgag atgcattgca ggcgttcttg aaagaaaaag
     1501 gaatcgcttc agaagtatat tatccgttgc cgccacatct atcggtgcca tgtcgaaagt
     1561 atggctacaa ggagggcgat ttctctcatg cagaaaaagc tgcaaaagaa acacttgccc
     1621 ttccgcttta ccctgagatg agtttggctc agcaagatga agtgatcgcg gctgtgaagg
     1681 aatttataaa tcaataggat gaatatgaaa ctagaaacga cagagcagga aaattggtcg
     1741 ctaatcatcc gtcctcaacg agcatggtgg gatctgcgtt tgggcgaatt gtggcgttat
     1801 cgtgatttgg tttggttgtt tgtttggcgt gattttgtcg catattacaa gcagacgatc
     1861 ttggg
//
LOCUS       DAOQNJ010000556         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_55162_length_1861_cov_7.535437, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000556 DAOQNJ010000000
VERSION     DAOQNJ010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55162_length_1861_cov_7.535437"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLG52_12860"
     CDS             <1..740
                     /locus_tag="PLG52_12860"
                     /inference="COORDINATES: protein motif:HMM:NF006202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="R2-like ligand-binding oxidase"
                     /protein_id="HNB42388.1"
                     /translation="LSMFVAGEEAVTLDLLPLIQAIAEEGRIEEEMYLTTFLFEEAKH
                     TDFFRRFMDEVAFPSPLGEGLGVGSDLSRYHGDNYRQLFYESLPEALNALRSDPSPAS
                     QIRASVTYNMIVEGVLAETGYQAFFTMLERNDLCPGLRKGIALLKQDESRHIAYGVYL
                     LSRLIAEHPQEWDNLQMQMNMLLPAAIGVIGDAFARYEVVPFGLKEEDFVNYAMSQFS
                     KRYERLEKARGASLDEINKVARENEEA"
     gene            741..1277
                     /locus_tag="PLG52_12865"
     CDS             741..1277
                     /locus_tag="PLG52_12865"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB42389.1"
                     /translation="MSLKPWKIIDSRHPMPKFRVDNCELPNGKPYKAFVLEFDSWANI
                     LAITKNNEVVLVKQYRHGVQELSLELPGGVVDEGESPLEGAKRELLEETGYQAGQIIE
                     VGRLYPNPAIQRNTLYCYLATDVEWVGEQDLDESEEIDVELVPLDEVVEMVRTGKFRH
                     ALNVAVIFHALIHLKKLT"
     gene            complement(1362..>1861)
                     /locus_tag="PLG52_12870"
     CDS             complement(1362..>1861)
                     /locus_tag="PLG52_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNB42390.1"
                     /translation="EKLYTNGITGGCQTSPLFYCPENTVTRAEMAVFLEKGIHNSSFN
                     PGNLSPTFNDTAGHWAEDWIEALKNDGITSGCATGLYCPDNSVTRGQMAVFLLKAKHG
                     AAYTPANVASTFSDTAGHWAEDWIQQLAAEGITSGCGSGIYCPENAVTRAEMAVFLVK
                     TFNLP"
BASE COUNT          448 a          432 c          498 g          483 t
ORIGIN      
        1 tgctttccat gttcgttgca ggcgaagagg cggtcacgct tgacctgctt ccgctgatcc
       61 aagcgattgc tgaagaaggg cgcattgaag aggaaatgta tctgaccacc ttcctctttg
      121 aagaagccaa gcataccgat ttcttccgtc gcttcatgga tgaggttgcg ttcccctctc
      181 ctttagggga ggggctaggg gtggggtcgg atttatcccg ttatcacggc gataactatc
      241 gccagctttt ctacgaatcc ctgcctgagg cgctcaatgc gcttcgctct gacccgtcac
      301 ctgcttctca aattcgggct tcggttacct acaatatgat cgtcgaaggc gtactggcag
      361 agacaggcta tcaagccttc ttcaccatgc tcgaacgcaa tgacctttgc cctggcttac
      421 gcaaagggat tgcattgctc aagcaggatg agtctcggca tattgcgtat ggagtttatc
      481 ttttatcccg cctcatcgct gaacatccac aggaatggga taacctgcaa atgcagatga
      541 acatgctcct gcctgctgcc attggtgtga tcggtgatgc gttcgcaaga tatgaagttg
      601 tcccatttgg tttgaaggaa gaggactttg tcaactatgc catgagtcaa tttagtaaac
      661 gctacgaacg ccttgaaaag gcacgcggcg ccagcttgga tgagatcaac aaagtggcaa
      721 gagagaatga ggaagcataa atgtcactaa aaccctggaa gatcatcgat tcgcgccatc
      781 ccatgccaaa gtttcgcgtg gataattgcg agctgccgaa cggaaaacca tacaaagcat
      841 ttgtcttgga gtttgactcg tgggcaaata tcctggcgat cacgaaaaat aatgaagtgg
      901 tcttggtaaa gcaataccgt cacggcgtgc aggaactttc gttggaattg cccggtggcg
      961 tggtggacga gggcgagagt ccgctggagg gggcgaagcg tgagttgttg gaagagactg
     1021 gttatcaggc tgggcagatc attgaggtgg gcaggttgta tcccaaccct gccattcaac
     1081 gaaatactct ctattgttac cttgctactg atgtggaatg ggtcggtgaa caagacctcg
     1141 acgaatctga agagatcgac gttgaactgg tacctctgga cgaagtggtc gagatggtgc
     1201 gtactggaaa attccgccat gccttgaatg tagctgtgat ctttcacgcg ttaattcatt
     1261 tgaaaaaatt aacttaattt gtttcctggc cattaacaac gtcggcctcc cgatttcagg
     1321 aggccgacac tgttttattc agtaaatacg taatgtgcct gttagggcag attgaatgtt
     1381 ttcacaagga agaccgccat ttcagcgcga gtgacggcat tctctggaca atagattccg
     1441 ctaccacagc cgctggtgat cccctctgca gccagttgtt gaatccaatc ttcggcccag
     1501 tgaccggccg tatcgctgaa tgtggaggca acattggcgg gtgtgtaggc tgctccatgt
     1561 ttggctttga gcaggaagac tgccatttgt ccacgagtga ccgagttatc cggacagtac
     1621 agtcccgttg cacaaccgct ggtgatccca tcgtttttaa gagcttcgat ccagtcttct
     1681 gcccaatgtc cggctgtatc attgaaggta ggagaaaggt tgcctgggtt gaaactagag
     1741 ttatggatac ctttttccag gaagaccgcc atttcagcac gggtaactgt attttcaggg
     1801 caataaaaca gcgggctggt ttggcagccg ccagtaatgc cattggtata gagtttttca
     1861 a
//
LOCUS       DAOQNJ010000557         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_55218_length_1860_cov_7.823823, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000557 DAOQNJ010000000
VERSION     DAOQNJ010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55218_length_1860_cov_7.823823"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLG52_12875"
     CDS             complement(<1..247)
                     /locus_tag="PLG52_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42391.1"
                     /translation="MTNETKQKVREFYDEIGWMQEEDGNYQNARYEDLRPVSREYIHK
                     TRLRVMNGLIPTGRFILDAGSGPVQYEEYKTYSAGYEK"
     gene            complement(257..>1860)
                     /locus_tag="PLG52_12880"
     CDS             complement(257..>1860)
                     /locus_tag="PLG52_12880"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42392.1"
                     /translation="LPFTAEIDWMLRSKNRPRKDHYNLTRLLTSLPDAVKVVKPYAES
                     APRGKNVLFFATLHYWIEQSAYLGLVLAGMGHDVTLLTLPYSEWHKEKDKFTQRQRIL
                     HTHDALKMLSPLVKHISMLDMQSSSRASHGTLHAHTASELPERIQTDIKEISLWDVQY
                     TLMREEVDMNDAGDRALYDLRVERNTFAAKAALQYMQANKPDVVLIPNGLILEMGIVF
                     RVARYLGIDAVTYEFNDQREQIWLAQNSSIMQQDTDYLVEARCSQPMTDAMFERVADL
                     ENARRGARVWGKSKRLWQYVSSQGADATRKQVGLDDRPVVMLAANVLGDSLTLGRDIF
                     ASSMTEWITKTVQYFAKRTDVQLVIRVHPGEKLVPQAKSMATVVKEALPELPSHIHVI
                     GALDKVNTYDLIEIADVGLAYTTTVGLETAMNGRPVISCGQTHYRGRGITIDPNTWDE
                     YYATLEKVLSNIPAHQMNEKQIEFAWNYAYRFFFEYPRPFPWRLMNFWDDLAEHPLES
                     VLSPEGMARFKKTFDFLVCEPFTWD"
BASE COUNT          484 a          464 c          431 g          481 t
ORIGIN      
        1 gcttttcgta gcccgcagaa taggtcttgt attcctcata ctggacagga ccagacccgg
       61 cgtcaagaat gaagcgaccg gtggggatga gtccattcat cacgcgcaga cgggtcttgt
      121 ggatgtattc gcgtgagacg gggcgcaagt cttcgtaacg ggcattctga taattgccgt
      181 cttcttcctg catccagccg atctcatcat aaaattcacg gactttttgt ttggtttcgt
      241 tagtcattta tttttcttaa tcccatgtaa aaggttcgca aaccagaaaa tcaaaggtct
      301 tcttaaatct tgccatgcct tcaggactta acacgctttc cagcgggtgc tcggcaagat
      361 catcccaaaa gttcatcaag cgccatggga agggacgtgg atattcaaag aagaaacgat
      421 aggcataatt ccatgcaaat tcaatttgct tttcattcat ttgatgggca ggaatattgc
      481 ttaacacttt ttcaagagtg gcatagtatt cgtcccacgt atttgggtca atcgtaatgc
      541 cacgcccacg ataatgggtt tgtccgcaag aaatgacggg gcgtccgttc atggcggttt
      601 cgagtccgac cgttgtggtg taggctaaac cgacatcagc aatttcgatt aaatcatacg
      661 tattgacttt atctaacgcg ccaatcacgt gaatatggct cggcagctcg ggcaaagctt
      721 ctttcactac cgtcgccata gacttggctt gcggcaccaa tttttcaccc ggatgaacac
      781 ggatcaccaa ctgaacatcc gtccgcttgg caaaatattg aacggtcttg gtaatccatt
      841 cggtcatgga agaggcgaag atgtcacgcc caagcgtgag gctatcaccc agcacgttcg
      901 cagcgagcat cactactgga cggtcatcca aacctacttg tttgcgagtg gcatctgccc
      961 cttgcgaaga gacatattgc cacaagcgtt tggacttgcc ccacacacgt gcaccacgac
     1021 gagcattttc aagatctgct acacgctcga acatagcgtc cgtcattggc tgagagcagc
     1081 gtgcctctac caaataatcg gtatcctgct gcatgatgga tgaattctgc gccaaccaaa
     1141 tctgttcgcg ttggtcgttg aattcatatg tgacggcgtc aatgccaaga taacgcgcca
     1201 cacggaacac aattcccatt tccaagatca aaccgttcgg gatcaaaacc acatccggtt
     1261 tattcgcttg catatattga agcgcggctt tggcagcaaa cgtattgcgt tccacacgca
     1321 ggtcatacaa agcgcgatcc cctgcgtcgt tcatatccac ttcttcacgc atcaaagtgt
     1381 attgcacatc ccaaagggaa atctctttga tatcggtttg aattctttca ggcaattcac
     1441 ttgcagtatg agcatgcaac gtcccatgag aagcacgcga agaggattgc atatctaaca
     1501 tggaaatatg cttcacgagc ggagataaca ttttcaatgc gtcatgcgta tgaagaatgc
     1561 gctgacgttg ggtaaatttg tctttttctt tatgccattc agaatagggc aaagtcaaca
     1621 gagtaacatc atgccccatg cctgccaaca ccaaacccaa atatgccgat tgttcgatcc
     1681 agtagtgaag cgtggcaaag aacaaaacat tttttccacg cggtgcgctc tcggcataag
     1741 gctttaccac cttcacggca tctggcaagg atgtgagcaa gcgcgtcagg ttgtagtgat
     1801 ccttgcgcgg acgattcttc gagcgaagca tccaatcgat ttcggcagtg aagggcaggt
//
LOCUS       DAOQNJ010000558         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_55538_length_1854_cov_7.900500, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000558 DAOQNJ010000000
VERSION     DAOQNJ010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55538_length_1854_cov_7.900500"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLG52_12885"
     CDS             <1..220
                     /locus_tag="PLG52_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42393.1"
                     /translation="DFANGHWHSAVADAACRCRAVNHSVACHIHGAFAKEGNDRAEYY
                     FDHDVNFRYGWALGIVFIETLEWKVQDV"
     gene            213..644
                     /locus_tag="PLG52_12890"
     CDS             213..644
                     /locus_tag="PLG52_12890"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNB42394.1"
                     /translation="MSDQVKEVLDWNRKIIEEFRENEGRVASFARQPLLLLTHTGAKT
                     GKQRTNPLAYFRDGDRYVIVASKGGAPTNPDWYYNLSANPRASIEVGNEQFEVVAEQA
                     EPVERERIFAMIVSLNPTFKDYENKAGRIIPLMILSPVLAD"
     gene            698..1537
                     /locus_tag="PLG52_12895"
     CDS             698..1537
                     /locus_tag="PLG52_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB42395.1"
                     /translation="MKKQFVLITGANDGIGLATAKAMLEKGFAVIVHARNEDKAKGGV
                     QRLEKEKAGAELIPVWGDLAHMDQVLRLAEQVKQVLPSGNGLDVLINNAGVYEDKRTL
                     TTDGFERTMGINHFAHALLTLKLHETLMAASAPRVLMVSGGVHIGAHLNFEDLDLAKG
                     WSSFGAYAASKLANSLFAAGLAQNKNWNGIWACSLHPGVIRTKMLIRVFGVGGEPLAE
                     GAKTSVYCATATGLESQNGGFFAHSRPAQGSPLIYNKQKVTEFWDLTLRRLKDYTANS
                     SLQ"
BASE COUNT          506 a          394 c          485 g          469 t
ORIGIN      
        1 tgattttgcc aatggtcact ggcattctgc cgtggctgac gccgcttgcc gctgtagggc
       61 tgtcaaccat tcagttgctt gccatattca cggtgcattt gccaaggaag gaaacgatcg
      121 tgccgaatat tattttgatc acgatgtcaa ttttcgttat ggttgggcgt tgggaattgt
      181 ttttatagaa actcttgaat ggaaggtgca ggatgtctga ccaagttaag gaagtattgg
      241 attggaaccg taaaataatt gaggagtttc gcgagaacga aggaagagtg gcgtcttttg
      301 caagacagcc actcttgctg ctcacccata cgggcgcaaa gacgggcaaa cagcggacga
      361 atccgctggc gtatttccgc gatggcgatc gttatgtgat tgtggcgtcg aagggtggcg
      421 cacctacaaa cccagattgg tattacaacc tgagtgcaaa tccacgtgca agtatagaag
      481 tgggcaatga gcaatttgaa gtcgttgccg agcaagccga acccgttgag cgtgagcgca
      541 tctttgccat gattgtttcc ttgaacccga catttaaaga ttatgaaaat aaagcaggta
      601 gaattatccc gcttatgatt ctgtcgcctg tgcttgctga ttaagaagat tcgaattgtg
      661 atgatggtta cacaaaaaac agtcgagata ataaattatg aaaaaacaat tcgtattgat
      721 tactggagcc aatgacggaa ttgggttggc tacagcgaaa gccatgctgg aaaaaggttt
      781 cgctgtcatc gtgcatgccc gtaatgagga caaggcaaag ggcggagtcc aaaggctcga
      841 aaaggaaaag gctggcgctg aactcattcc agtttgggga gacctggctc atatggatca
      901 ggtcttgagg ttagctgaac aagtaaagca agttttgcct tcagggaacg ggctcgatgt
      961 cttgatcaat aatgccggtg tttatgaaga caagcgcaca ttgactaccg atggctttga
     1021 acgcaccatg ggcattaatc atttcgcgca tgcccttctg acgcttaaat tgcacgagac
     1081 tctgatggca gcctccgctc ccagagtctt gatggtcagc ggaggtgtgc atatcggcgc
     1141 gcatctgaat tttgaggacc tggacttggc taaagggtgg agttcttttg gcgcttatgc
     1201 tgcttcaaaa ttagccaaca gcctttttgc cgcgggacta gcccagaata agaactggaa
     1261 tggaatctgg gcttgcagcc tgcatcccgg agtcattcgc actaaaatgc ttataagggt
     1321 ctttggtgta ggtggtgaac cactggctga aggagcaaag acttcggttt actgcgccac
     1381 agcgaccggt ttggaaagtc aaaatggagg attctttgca cacagccgac ctgctcaggg
     1441 aagcccactc atctataaca agcaaaaagt gactgaattc tgggatttga ccctgcgccg
     1501 tctgaaagac tatactgcaa acagcagttt gcagtaactg tgaatcacga ttaatgagat
     1561 gaacttagca agcaaggtaa tcaaagagta cgagaagaaa tcatgtagaa ccattctact
     1621 gataaaccta tcgcctgcgg tcttcgtcca aaaaatcact tcgactacaa tatcacccac
     1681 cttacactta cagaaagaat cgcattatgc aaactattga atttggatta gacaccttcg
     1741 gcgatgtgcc aatggatgac tctggcaaac ccgtttcgta tgcaagggcc atccgtcaag
     1801 tcgttgatga ggctgtattg gcagactccg tcggcgtgga tgtgatcgcg ctgg
//
LOCUS       DAOQNJ010000559         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_56259_length_1840_cov_6.622409, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000559 DAOQNJ010000000
VERSION     DAOQNJ010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56259_length_1840_cov_6.622409"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..>1840)
                     /gene="topA"
                     /locus_tag="PLG52_12900"
     CDS             complement(83..>1840)
                     /gene="topA"
                     /locus_tag="PLG52_12900"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNB42396.1"
                     /translation="AFSHPRNIDMNLVNAQQARRVLDRLVGYSISPILWEKVRGRLSA
                     GRVQSVALRLIVDREREIDAFKPVEYWTIHAEFKPEKLKSNFIAKLVRVDDKEPELPS
                     KETVEPLLYDLETASFSISKVKRGERRRKPAAPFTTSTLQQEASRKLGYTARRTMALA
                     QGLYEGQDVGEGGTTGLITYMRTDSTNVSAIAQSETREFVTGKYGGDFLPAEPPTYKT
                     KSANAQEAHEAIRPTSVMRDPEKVKEFLEPAMYKLYRLIWQRFVASQMEAAVYDTLQV
                     EVTGKTNEHEYLLRASGSAIKFPGFMVVYEEAKNEDAKVEEDEEDVKIPAGVAEGQKQ
                     ELVRLIPEQHFTQPPPRFSEASLVQALEENGIGRPSTYAPTISTIQQRGYALREEKRL
                     VPTDMGIQVNDLMAQYFPEVVDLNFTAHMEEDLDKIADGQMEWVDAIREFYGPFAEDV
                     KKAQAEMPVAKSEPEPIGRKCPDDGGELVIRYGRFGKFISCANFPTCRYTEPWLEKIG
                     VSCPKDGGDMVERRTRKGRTFYGCANYPNCDFTTWKKPLAKPCPKCNGLLVIANKREA
                     QCTNCSESFLLEEIVPENA"
BASE COUNT          451 a          485 c          432 g          472 t
ORIGIN      
        1 attgcccaca tggataaaaa aacgcatcag actcagccat acttgccact acgaccaagc
       61 ctgatgcgaa cactgaaagt ttttatgcgt tctctggcac gatttcttcc aacaagaaac
      121 tttcagaaca attcgtgcac tgcgcctcgc gtttgtttgc tatcaccaac aacccattac
      181 atttcgggca aggtttggct aagggcttct tccatgttgt gaaatcacaa ttcgggtaat
      241 tcgcacaacc atagaaggtg cgtcccttgc gagtgcggcg ttccaccata tcgccgccat
      301 ctttcgggca ggaaacaccg atcttctcca accacggttc tgtatagcga caggttggga
      361 agtttgcgca ggaaatgaac ttgccgaagc gcccataacg gataactagc tcaccgccat
      421 catccgggca ttttcgtcca atcggctccg gctcagattt agcaacgggc atttctgcct
      481 gtgccttttt cacatcctca gcaaacggac cgtagaactc acgaatggca tccacccatt
      541 ccatttgtcc atccgcaatt ttatcgaggt cttcttccat atgagccgta aagttcaaat
      601 cgactacttc agggaagtat tgagccatca agtcattgac ctgaatcccc atatcggttg
      661 gcaccagtcg tttttcttcg cgcaaggcat atccgcgctg ctgaatggtt gaaatagtcg
      721 gcgcataggt agacggacga ccaatcccat tctcttcaag ggcttgtaca agcgaggctt
      781 ctgaaaagcg tggaggaggt tgagtaaagt gctgttcggg aattaagcgg accaattcct
      841 gcttttgtcc ttccgccaca ccggcgggaa tctttacatc ttcttcgtct tcttcaacct
      901 tggcatcttc atttttggct tcttcgtaaa caaccataaa gccagggaac ttaatagcag
      961 acccagaagc acgcaagaga tattcgtgtt cgttcgtctt gccagtcact tcaacctgca
     1021 aagtgtcgta tacagcggct tccatctgag aggcaacaaa gcgttgccag atcaagcgat
     1081 atagcttgta catggcgggc tcaaggaatt ccttgacctt ttccgggtca cgcatcacag
     1141 atgtagggcg aatggcttcg tgagcttcct gtgcattcgc agacttggtt ttgtatgtag
     1201 gcggttctgc cggaagaaag tcgccgccat atttaccggt gacaaactct cttgtttcgc
     1261 tttgagcaat agcagagaca ttagtcgagt cggtacgcat ataagtgata agacctgtcg
     1321 taccaccctc gcctacatcc tgaccttcat aaagcccctg cgccaacgcc atggtacgac
     1381 gggctgtgta gcctaattta cgggacgctt cctgttgtaa cgtagacgtt gtaaatggcg
     1441 cagccggttt gcggcgacgc tctccgcgtt tgacctttga aatggaaaaa gaagcagttt
     1501 cgaggtcgta aagaagcggc tctacagtct ccttgctggg taattccggc tctttatcat
     1561 ccacacgcac gagtttggcg ataaagttcg attttagttt ttcaggtttg aattcagcat
     1621 ggatcgtcca atactcaacc ggtttgaaag catcgatctc gcgctcacgg tccacgatga
     1681 ggcgcaaagc gacagactgc acacgacctg cagagaggcg tccgcggacc ttttcccaca
     1741 aaatagggct gatgctatag cccacaagac gatccaacac gcggcgcgcc tgttgggcat
     1801 tgaccaagtt catgtcaata ttacgcggat gcgagaaagc
//
LOCUS       DAOQNJ010000560         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_56438_length_1837_cov_5.423120, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000560 DAOQNJ010000000
VERSION     DAOQNJ010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56438_length_1837_cov_5.423120"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLG52_12905"
     CDS             complement(<1..273)
                     /locus_tag="PLG52_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNB42397.1"
                     /translation="MSYSLSFSQAIFTVLFVGDKVEQGFFDFVPTKELSAALNIPNPT
                     AVKILGSLANAGIIETREGSRGGVRLAKPAKQITLLDVFNAIEAGKP"
     gene            complement(366..1361)
                     /locus_tag="PLG52_12910"
     CDS             complement(366..1361)
                     /locus_tag="PLG52_12910"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNB42398.1"
                     /translation="MKKVVITEHGSPAVLQIIETPIPEPKANEVRIKVSVTDITWADT
                     MARRGQYPTKNKIPYAPGYDIVGAVDKIGAGVSSSVLNQRMAALLPNMGAYAEYVCVP
                     QSLLVPVPDQLDSAQAVGVILNGLTAYAALHKFAKIKAGEKILITGAAGGLGTMLIQL
                     AKLAGLDVYGSASTGKLTLVQELGATPLDYKTEDIPARIQQLSNGGVDVVIDLAGESS
                     AALSSLCDGGRIITVGSLSMKDKSPMQMLAGLFGTVYRSLMNPKKKIKFFGSLPPMVE
                     KDPAWYRATMSKLFNLVVEQKLKVIIAKRLPLIEATQGHALLESGTVSGKIVLEI"
     gene            complement(1409..>1837)
                     /locus_tag="PLG52_12915"
     CDS             complement(1409..>1837)
                     /locus_tag="PLG52_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42399.1"
                     /translation="FRCSWFMETLPLFVQGKSISLIGNQIHPLHWIAVADYARMVSKS
                     YQTDETLNKELYIFGPEAFTMGEAMKIYADHAGIKVAPMSTTMLSVLGTLTFNTEWKG
                     MSVLMKHYENWGEDGSPEEANRLLGTPRITLKEWCEKNRR"
BASE COUNT          410 a          468 c          455 g          504 t
ORIGIN      
        1 gggcttgccc gcttcgatgg cgttgaacac atcgagcaag gtgatctgtt tggcgggctt
       61 tgccagccga acgccgccac gtgagccttc gcgcgtttcg atgatgcccg catttgccag
      121 gcttcccaat atcttgaccg ccgtagggtt ggggatattc aacgcggcag ataattcctt
      181 cgttgggaca aagtcgaaaa agccttgttc aactttgtcg ccgacaaaaa ggacggtgaa
      241 gatggcttgc gaaaaagata atgagtaact cataactata tatagtttat atttagttta
      301 ttgatttgtc aatagggagt tccgtgcgct ttgtcttcag atcaaaggaa aaatatctgt
      361 tttttctata tttccagcac gatcttcccg ctgaccgtgc cgctttccaa caaggcatgt
      421 ccctgagtgg cttcgatcaa cggcaggcgc ttggcgatga tgactttcaa tttttgctcc
      481 accaccaagt tgaacaactt gctcattgtc gcacgatacc aggcggggtc cttctctacc
      541 attggcggca ggctgccaaa aaatttgatt ttcttttttg gattcatcaa cgagcgatag
      601 actgtgccaa acaagcctgc cagcatttgc atgggacttt tatctttcat ggacaaactt
      661 ccaacggtga tgattctgcc gccatcacac agagaactca atgcagcgga tgattctccg
      721 gcaaggtcaa tcacgacatc cacgccgcca ttcgagagtt gttgaatccg tgcggggatg
      781 tcttcggtct tgtaatccag cggtgtcgcg cccagttcct gaaccagagt caacttgcct
      841 gtcgaagcag acccgtacac atccaagccc gcaagctttg cgagttgaat caacattgtg
      901 cccaatccgc ctgccgcgcc ggtaatcaaa attttttcgc ccgctttgat ttttgcaaat
      961 ttatgcagcg cggcatacgc agtaagcccg ttgagaatca cgccgacggc ttgcgcggaa
     1021 tcaagttgat cggggacagg caccagcagc gactgcggca cgcaaacata ttcggcatag
     1081 gcgcccatgt ttggcaaaag cgccgccatg cgttgattta atacggacga agatacgcct
     1141 gcgccaattt tgtccaccgc gccgacgatg tcatagccag gcgcgtaggg gattttattt
     1201 ttggtggggt attgtccgcg ccgcgccatt gtatctgccc aggtaatgtc ggtgacgctg
     1261 accttgatcc gtacttcatt tgcttttggc tcaggtatgg gcgtttcgat gatttgcagc
     1321 actgcgggtg aaccgtgctc ggtaatgact acctttttca tttcaattct tctcctgttt
     1381 tatacgcaga agtatggaat ttgatgcact acctgcgatt cttttcgcac cactctttca
     1441 gtgttattcg tggcgttccc agcaatcgat tggcttcttc gggcgagccg tcttcgcccc
     1501 agttttcgta atgcttcatc aacacagaca tgcctttcca ttcggtgttg aaggtcagcg
     1561 ttcccaacac ggataacatc gtggtggaca tcggcgcgac ttttatgcct gcgtggtcgg
     1621 cgtaaatttt catcgcttcg cccatcgtga aggcttcggg tccgaaaatg taaagttcct
     1681 tgttaagtgt ttcgtcggtc tgataacttt ttgaaaccat gcgggcataa tctgccacgg
     1741 cgatccaatg cagcgggtga atctgattcc cgatcagcga aatgcttttg ccttggacga
     1801 agagtggcag agtctccata aaccatgaac agcggaa
//
LOCUS       DAOQNJ010000561         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_56931_length_1828_cov_6.026509, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000561 DAOQNJ010000000
VERSION     DAOQNJ010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56931_length_1828_cov_6.026509"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLG52_12920"
     CDS             complement(<1..488)
                     /locus_tag="PLG52_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42400.1"
                     /translation="MKSMSRMLSLTLLLLGLMALVWACAPAEESIAVEPVELEPQAIV
                     DAVTKGGCSACHAIPGIPGAVGVIGPDLASISTVAAEHIADGSYTGKAKTAEEFILES
                     ITNPEAYLSQHCPAGMCQPGLMPATLKDTLTSEEINLIVGYLATLPGGESIMTDANVA
                     VDT"
     gene            complement(807..1508)
                     /locus_tag="PLG52_12925"
     CDS             complement(807..1508)
                     /locus_tag="PLG52_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB42401.1"
                     /translation="MNISLNDLRKISVFQHATDADLDLIFKNSIVRSIEEGEYFFLQG
                     DETKYLYVLVTGQVKLMQSNPNGQQVNLRTIYPWQMFGALGATRGEDAIYPAMAQTFE
                     CSTAIAIPSHFLRTMMETRSYLSFDLMNLMTTYIQELQTRYREIATNRVEQRIANALI
                     RLAQQSGIRSGKDAGIELFFSRKDVSEMTGATVFSVSRLISEWERKSIIKAGRERIQI
                     LDPHALMQIAEGHKE"
     gene            complement(1505..>1828)
                     /locus_tag="PLG52_12930"
     CDS             complement(1505..>1828)
                     /locus_tag="PLG52_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42402.1"
                     /translation="FGAQVAGPRYDAVLHAVFVGFVISMIFGHAPIIFPAILGVPIDF
                     KPAFYIHLGLLHGSLAVRVIADYANLHTLRMWSGLLNEIAILVFIGMTVHSVLQSRKT
                     ELKTP"
BASE COUNT          476 a          428 c          433 g          491 t
ORIGIN      
        1 gtatccactg caacatttgc atctgtcatg atcgattcac cacctggcag ggtcgcaagg
       61 tagccaacaa tcaggttaat ctcttcactg gtgagcgtat cttttagcgt ggcgggcatc
      121 agaccgggtt ggcacattcc agccgggcag tgctgcgaaa gataggcttc cgggttggtg
      181 atcgattcga gaataaattc ctcggcagtc ttagccttgc cggtgtagct gccatccgca
      241 atgtgttctg cggcgacggt ggaaatactt gcaaggtcag gaccaattac gccgacagcg
      301 cccgggatgc cggggatggc atgacaggcg ctgcatccac ctttggttac ggcgtctaca
      361 atggcttgcg gttcgagttc cactggttca acagcgatgg attcttcagc aggcgcgcac
      421 gcccaaacca gagccattaa ccctagcagc agcagggtaa ggctcaacat acgagacata
      481 cttttcatat cattctcctc gttcggaagg ttgtttttgt ttgaggctta tggatagtac
      541 attcaagaag tctttttcat cgagttgata tgactcaacg acataagcaa gcgtgcaaaa
      601 tcgcgcaaaa tggcagccca cacatgctgt tttgaattga ttaaaaacaa caagcgtttg
      661 gggagccttg cttaaagtgt ttgccacagt atcttcgtga atgtttagcg cctcattcat
      721 cggtgcgtac aataatttat ttccccatct ttttcattgc gatagcgcaa tttacgccta
      781 gttagttatt tgtttcacaa tgtcacttat tctttatgcc cctccgcgat ttgcataagg
      841 gcgtgtggat ccaaaatttg aatacgttcc ctgcctgctt tgataatgct ctttcgttcc
      901 cattcactga tcaagcggct gaccgagaag acagttgccc cagtcatttc cgaaacatct
      961 tttcgagaaa aaaataactc aattcctgca tctttgcctg aacgaattcc agactgttgc
     1021 gcaagacgaa ttaatgcatt agcgattcgc tgttcgactc gatttgtggc aatttcccga
     1081 tatctagtct gcaactcttg aatataagtg gtcattaagt tcattagatc aaaagacagg
     1141 tatgagcggg tttccatcat ggtgcgcagg aagtggctgg gaatggcaat cgctgtactg
     1201 cattcaaacg tttgagccat tgcaggataa attgcgtctt ccccacgcgt agcgcccaat
     1261 gccccaaaca tctgccacgg atagatcgtc cgtaaattga cctgctgccc attgggattt
     1321 gattgcatca gtttgacctg tccagtgacc aacacgtaca ggtatttggt ttcgtcgcct
     1381 tgaagaaaga aatattctcc ttcttcaatg gaacgtacga tgctgttttt aaagatcaaa
     1441 tccaaatcag cgtctgttgc atgttgaaag acactgatct tgcggagatc gttcaaagat
     1501 atgttcatgg tgtttttaat tctgtttttc tgctttgtag aacagagtga accgtcatgc
     1561 caataaaaac gaggatggca atttcattca gcagcccact ccacatgcgc aatgtatgca
     1621 ggttggcata atccgcaatg acgcgaacag caagcgagcc atgcaataac ccaaggtgaa
     1681 tataaaaagc gggcttaaag tcaatgggta cgccaagaat ggcggggaag atgatcgggg
     1741 cgtgaccgaa gatcatggag atgacgaagc cgacaaaaac ggcatgaaga accgcatcgt
     1801 atcttggacc tgccacctgc gcgccaaa
//
LOCUS       DAOQNJ010000562         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_57200_length_1823_cov_10.143100, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000562 DAOQNJ010000000
VERSION     DAOQNJ010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57200_length_1823_cov_10.143100"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PLG52_12935"
     CDS             complement(<1..604)
                     /locus_tag="PLG52_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB42403.1"
                     /translation="MNERILIIEDDQAILKLLQRGLAYEGYTVDTATDGRMGLIAARD
                     HTPDLVVLDWMLPGMDGLEVCHRLRQGGSIPILMLTAKDTVQDRIQGLDAGADDYMVK
                     PFNLDELLARVRALLRRTQPERIPVLKFADLSLDTGSRQATRGNRTVALTAKEYELLE
                     LFLRHPKQVLTREVIFDRVWGYDFGGESNVLEVYIRYLRQK"
     gene            complement(647..1297)
                     /locus_tag="PLG52_12940"
     CDS             complement(647..1297)
                     /locus_tag="PLG52_12940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB42404.1"
                     /translation="MKILHRDSSIVVVDKPADLSVLAEGWSKDAPYLAKILEEQFGEI
                     WVVHRLDKGTSGVIVFALTAEAHRSLNIQFEKHEVEKAYHALVNGIPKWDEKVTKFPL
                     RVNVGHKHRTVVDDRNGVPAETRFRLLERYQAAALVEASPMTGRTHQIRVHAYALGYP
                     LVGDILYSAPETNLIARPALHARSLTLTHPENGKRLTFQADYPNDLLNAINLLRGK"
     gene            complement(1278..>1823)
                     /locus_tag="PLG52_12945"
     CDS             complement(1278..>1823)
                     /locus_tag="PLG52_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42405.1"
                     /translation="DYYMTLSSAKGYKEMSVMRFENSFELNPAFQKDIDSQTKVSSRH
                     VTFLILYTAAILFLWFVAVRMFNAAWAYSFFIGMLVLMEVTIHMRHFRNWYFLRVFRS
                     KGGITGGITYAQWFSYQVSAFEFYCFAALYLFFFLLTYSFFFLGGVMICLRIGVSHSN
                     LAKKAERQHSASVAKNENSSS"
BASE COUNT          533 a          477 c          369 g          444 t
ORIGIN      
        1 acttttggcg cagataacga atgtacactt ccaacacatt gctctcgccg ccaaaatcgt
       61 agccccacac acggtcgaag atcacttcac gagtgagcac ttgcttaggg tggcgcagga
      121 ataattccag taattcgtat tccttcgcgg tcaaagcgac ggtgcgattc ccgcgagttg
      181 cctgacgtga ccccgtatcc agtgacaagt ctgcaaactt caaaacagga atacgttctg
      241 gctgggttcg gcgcagaagc gcacgcacac gtgccaataa ctcatccagg ttaaacggtt
      301 tgaccatgta atcatccgca ccggcatcca atccctgaat gcggtcttgc acggtatctt
      361 tggcagtcag catcaaaatc ggaattgaac cgccctgtcg caaacgatgg cacacttcca
      421 aaccatccat gccgggtaac atccaatcca ggacgaccag atcgggggta tggtcgcgag
      481 ccgcgatcaa tcccatgcgt ccatctgtgg cggtatctac cgtataacct tcatacgcca
      541 gaccgcgctg taacaacttt aagatagctt gatcgtcttc gatgatgagg attcgctcgt
      601 tcatggctat actccttact aaaaatatac cataaattca agaggcttat ttgcctctca
      661 gcaaatttat agcattcaat aaatcattag gatagtctgc ttgaaaggtc aacctcttac
      721 cattctcagg atgtgttaat gtcaacgatc gtgcatgcaa tgccgggcgc gcgatcaagt
      781 tagtttccgg tgcgctataa aggatatccc ccaccagtgg ataacccaac gcataggcat
      841 ggacacgaat ctgatgcgtt ctccccgtca ttggcgacgc ctctaccaac gccgcagctt
      901 gatagcgttc cagcagccta aacctcgtct cagccgggac gccattgcga tcatccacca
      961 cagtgcgatg tttatgcccc acattcacac gcaatggaaa tttcgtcacc ttctcatccc
     1021 attttggaat accgttcacc aaggcatgat aggctttttc cacttcatgc ttctcaaact
     1081 gaatattcag cgaccgatga gcctccgccg tcagtgcaaa gacgatcact ccgcttgtgc
     1141 ctttatccaa acgatgcacc acccaaattt cgccaaattg ttcttccaaa atttttgcaa
     1201 gatacggtgc atccttactc cacccttccg caaggacaga gagatcggct ggtttatcaa
     1261 ccactacaat agacgaatca cgatgaagaa ttttcatttt tagctacaga ggcagagtgt
     1321 tggcgttcag ctttctttgc cagattggaa tgagacactc caatacgtaa acaaatcata
     1381 accccgccta aaaagaaaaa gctgtaggta agtaagaaga agaacaaata caaggcggca
     1441 aaacaataga attcaaaagc agatacctga tatgaaaacc actgagcata cgtgatacca
     1501 cctgtgattc cgcctttgct acgaaatacc ctcaaaaaat accaattacg aaagtgacgc
     1561 atatggatgg tcacctccat gagaacaagc ataccgatga aaaaggaata tgcccacgct
     1621 gcattgaaca ttcttacagc aacaaaccat agaaaaagaa tggcagctgt atacaagatc
     1681 aagaacgtga cgtgtcgact tgaaactttg gtttggctat caatatcttt ttgaaatgcc
     1741 gggtttaatt caaagctatt ctcaaagcgc atcacactca tttctttata tccctttgca
     1801 gaagaaaggg tcatgtaata atc
//
LOCUS       DAOQNJ010000563         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_57301_length_1821_cov_7.954134, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000563 DAOQNJ010000000
VERSION     DAOQNJ010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57301_length_1821_cov_7.954134"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PLG52_12950"
     CDS             <1..1235
                     /locus_tag="PLG52_12950"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNB42406.1"
                     /translation="LYSLRLRTPPPTATLSNKPAPKSNRFLWIGAGILACLCLGVVAT
                     GAVGFSIYSLFPKATNTPQILTDAATPDHMSHGSTAEPGSTPQLISFGEFFGVLRFQG
                     NMDQVTVTASLPDPAEGTQYEAWLIDDGGELLISLGILEKNNSGQYNLTFTDPESRNL
                     LDGFNRMEITLETNPDASPNPSDTILYSSGIPAGALTHVRHLLVSFPSNPNQTPMTGG
                     LMGTSSLINEAGTALEKAFEKGDTTAIRLQAETLINLIVGKQSNEYKDWDKSGKISDP
                     SDGFGLLLNGGQTGYIEGTITHAELSASAGDATANIKLHSEHVVISAKNVEGWATQLR
                     DVAKRILESDQVSESDIRLAVSLAEQIYQGVDIDGNESVDPVEGEGGALTAYQHAEYM
                     ADMQVLEGANLFPPPGNP"
     gene            1355..>1821
                     /locus_tag="PLG52_12955"
     CDS             1355..>1821
                     /locus_tag="PLG52_12955"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB42407.1"
                     /translation="MTIVTAETFVQAPLKFAYRAFTNATTIREWLCDVATVAAHPRGR
                     MYLWWAGDFYSSGHYLELEENKRVKFRWYSSIDPAPTEVTVTFTEKDGGVNVRMDHEV
                     PSDEAWKKMAEGFRNNWQDSLRNLKSVLETGIDLRIAERPMLGIVPGDFSPEQA"
BASE COUNT          470 a          465 c          461 g          425 t
ORIGIN      
        1 ctttatattc cctgcgcctc cgcaccccgc cgccaactgc cactcttagc aataaacctg
       61 ctcctaaatc aaatcgcttc ctctggatcg gcgccgggat tctggcatgt ttatgtttag
      121 gggttgtcgc cacaggcgcg gtcggattct ccatttattc cttgtttccc aaagcgacca
      181 ataccccgca aattctcacc gatgcagcca caccagacca catgtcgcat ggcagcacag
      241 cagaacccgg ctccacgcct cagcttatct cttttggtga attctttggg gtgttacgct
      301 tccaaggcaa catggaccaa gtcacagtca ccgcctccct gccagacccg gcagaaggca
      361 cgcaatatga agcctggctg atcgacgatg gcggcgaatt actcatcagc cttggaatct
      421 tggaaaagaa taattccggt caatacaatc tgacgtttac tgaccctgaa agccgcaatc
      481 tattagacgg tttcaatcgc atggaaatca cgctcgaaac caatccagat gccagcccca
      541 acccatccga cacgatcttg tattcctctg gcatccccgc cggtgcgttg actcacgttc
      601 ggcatttgtt agtcagtttc cccagcaacc ccaaccagac ccccatgacg ggcgggctga
      661 tgggaaccag cagtctgatc aatgaggctg ggacagcgct ggagaaagcc tttgaaaaag
      721 gcgatacgac tgcaattcgc ttacaagccg agacgttgat caaccttatt gtgggcaagc
      781 aaagcaacga atacaaagac tgggacaaga gcggaaagat cagtgaccct agcgatggct
      841 ttgggctttt gttgaacggt ggtcagaccg gttatattga agggacgatc acccatgccg
      901 aactttctgc ttcggcgggc gatgccactg caaatatcaa acttcacagt gagcatgtcg
      961 tgatcagcgc gaaaaatgtt gagggatggg caacccagct tcgagatgtc gcgaagcgga
     1021 ttctcgagtc agaccaggtt tctgagtccg atatccgctt ggcagtttca cttgcagaac
     1081 agatttatca gggtgtggat attgacggca acgaaagtgt ggatccggtt gaaggggaag
     1141 gcggggcatt gacggcatat caacatgcag aatatatggc agatatgcag gtccttgaag
     1201 gcgcaaatct tttcccacca ccagggaacc cgtagagtaa acgtcgtgcc tttcgcacga
     1261 cgtttttatt tcaatagaaa acccatgcca gagtaggttg aatcttgttg gcaatcgtta
     1321 tataaagaat aaatacttac tcgaggagag tttcatgact atagtaactg ctgagacctt
     1381 tgtacaagcc cctttgaaat ttgcctaccg agcctttacc aatgccacaa ccattcgcga
     1441 atggttgtgt gatgttgcaa ccgtcgctgc gcacccacgt ggacggatgt atctgtggtg
     1501 ggctggcgat ttctattcca gcgggcatta tcttgaacta gaggaaaaca aacgggtgaa
     1561 gttccgctgg tattcaagca tcgaccccgc tcccactgag gtgaccgtga cgttcactga
     1621 gaaagatggg ggcgtaaacg tccgtatgga ccatgaagta ccttctgatg aagcatggaa
     1681 gaaaatggcg gaaggttttc ggaacaactg gcaagatagc ttgcgaaatt tgaagtctgt
     1741 gcttgaaacg ggcattgact tgcgcattgc agaacgcccg atgttgggaa ttgtgccagg
     1801 cgactttagc ccagaacaag c
//
LOCUS       DAOQNJ010000564         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_57429_length_1819_cov_7.360544, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000564 DAOQNJ010000000
VERSION     DAOQNJ010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57429_length_1819_cov_7.360544"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1087)
                     /locus_tag="PLG52_12960"
     CDS             complement(41..1087)
                     /locus_tag="PLG52_12960"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNB42408.1"
                     /translation="MTTLITPSVPHSDKKIFVSLAAALVLWLAAYNLIQPIANWISFD
                     LLKLSPDSQLGESLAFFLYDVPKILLLLSGMVFLISIVRTFFSPERTRALLGGKREGV
                     GNIFAALLGIVTPFCSCSAVPLFIGFVEVGIPLGVTFSFLIAAPTINEVAIILLFGLF
                     GWKVAGLYVASGLVVAIVAGFIIGRLKMEKYVADFVWKVHRKVNVTEEKLTWSDRIER
                     AWQSVKEIVGKVWLYVVVGIAVGAGIHGYVPEDALASILGAQAWWSVPLAVLIGIPLY
                     SNAAGIVPIVGTLIEKGASVGTALAFMMSVIGLSLPEAVILKRVLKMPLLVTFFVVIA
                     IAIIFTGYLFNLIL"
     gene            complement(1090..1479)
                     /locus_tag="PLG52_12965"
     CDS             complement(1090..1479)
                     /locus_tag="PLG52_12965"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB42409.1"
                     /translation="MKKQPFAQVSKQIAEPLSAIASPQRIAILLAIGKGEACVCHLET
                     ALGLRQAYISQHLMALRKADILQDRREGRYVFYKIKNASLLDLITASASLSGLSAESV
                     SSLINTQVYPSCECPHCVPALIPIASL"
     gene            complement(1611..>1819)
                     /locus_tag="PLG52_12970"
     CDS             complement(1611..>1819)
                     /locus_tag="PLG52_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42410.1"
                     /translation="ALRESLPPLTKPKPETRPEGFPEGQGGWPLYFAPIGFRNNRAFG
                     SLFMLGLLLDVAIRLIPATQNFWR"
BASE COUNT          545 a          452 c          454 g          368 t
ORIGIN      
        1 cctgagccta gaactttgat ggttaacatt tcattttcct ttacaagatc aaattgaata
       61 aatagccagt gaagatgatc gcaatggcga tcacgacaaa gaacgtgacc aaaagcggca
      121 tcttcaaaac acgtttgaga atcaccgctt cgggcaaact taagccgatg acagacatca
      181 tgaatgccag agctgttcca actgaagcgc ctttctcgat caacgttcca acgatgggaa
      241 cgatccctgc cgcattggaa tacaacggaa taccgatcaa caccgccaac ggaaccgacc
      301 accacgcctg cgcccccaaa atagatgcca atgcatcttc tggcacgtag ccatgaatgc
      361 ccgcccccac ggcaataccc accaccacat acaaccagac cttgcccacg atctctttca
      421 ctgattgcca tgcgcgttcg atacgatcgg accaggtcag tttttcttcg gtcacattca
      481 cttttcggtg aaccttccag acaaaatctg ccacatactt ttccattttg agtcgcccaa
      541 taatgaagcc tgccacgatg gcaacgacca accccgaagc gacatacaac cctgctacct
      601 tccagccgaa caagccaaac agcaagatga tcgccacttc attgatggtc ggtgcggcga
      661 taaggaagga gaaagtcaca ccaagcggaa tacccacttc aacaaagccg atgaaaagcg
      721 gaacagccga acatgagcag aacggggtga caattcccag cagagcagcg aaaatgtttc
      781 ccacaccttc tcgcttgccg cccagcaagg cacgagtccg ctcggggctg aagaaggtgc
      841 ggacaatcga tatcaggaag accatacccg aaagcaaaag caggatcttc ggcacatcgt
      901 agaggaagaa agcaagagac tcgccaagct gtgaatcagg ggaaagcttc aagagatcga
      961 aagagatcca atttgcaatg ggctgaataa ggttatatgc agccagccat agaacaaggg
     1021 cggcagccag agaaacaaat atctttttat cagaatgcgg aacagatggg gtaatgagtg
     1081 tggtcatggt tacaaactcg ctatgggaat taaagcaggc acacaatgcg gacattcaca
     1141 cgatggatat acctgcgtat tgatgaggga agaaacagat tcggcggaga gtccgcttag
     1201 ggaggcagag gcagtgatca ggtccagcag agaggcgttc tttatcttgt agaaaacata
     1261 acgcccctcg cggcgatcct ggaggatgtc cgctttgcgg agcgccatca aatgctgaga
     1321 gatgtaagct tggcgtaatc ccagcgcagt ttcaaggtga caaacgcaag cttcgccttt
     1381 gccaatggca agcagaatgg caattcgctg aggagaagca atagcagata aaggttctgc
     1441 gatttgtttg gagacttgag caaagggttg ttttttcata ttcataaacc tgaatatatt
     1501 gtaggctcac attcaaaata ttgaatgtga cgattgtcat cgaaacatca cctctcccct
     1561 gacaacaaaa aagcctcccg gtattgggag gctttgaagt acagattgtt ttagcgccag
     1621 aaattttgtg tggcagggat caggcgaatt gccacatcca gcaagagacc gagcatgaag
     1681 agcgagccaa aggcgcggtt gttgcggaag ccaatgggcg cgaaataaag aggccagcca
     1741 ccctgtcctt cagggaaacc ttcagggcga gtttcgggct tgggcttggt gagaggcgga
     1801 aggctctcgc gtaaagcag
//
LOCUS       DAOQNJ010000565         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_57708_length_1814_cov_10.214895, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000565 DAOQNJ010000000
VERSION     DAOQNJ010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57708_length_1814_cov_10.214895"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLG52_12975"
     CDS             complement(<1..214)
                     /locus_tag="PLG52_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNB42411.1"
                     /translation="MKISNRFNPNADCILFEGDCLDLLKTIPDNTVKLVVTSPPYNLG
                     KEYETRLHLQKYLDQQKKIITECVRIL"
     gene            complement(262..1260)
                     /locus_tag="PLG52_12980"
     CDS             complement(262..1260)
                     /locus_tag="PLG52_12980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNB42412.1"
                     /translation="MKLDAAIPPVQLKQIPAIATAAEETGFAALWTQETQHDPFLPCT
                     LIAEHSANMEIGTAIAVSFARSPANLAYTAWDLAAQSNGRFILGLGTQVKAHIERRFG
                     MTWPESVTGKLREQIQVIRALWDAWQNGSKLNFRGEHYKITLMSPFFNPGKIDNPNIP
                     IYIAGVNQGLARLAGEMCEGFHAHPFNSPRYMNEVLLPAIEEGLQKSGRKRSDLAVSM
                     TPFIATTPEEEGFARMQIAFYASTPSYRSVMDLYGWSETAEKLSGFAAKGEWHEMPML
                     ITDEMLNEFCLMTTQENLATDLKKRYDGIADRLTLYTPFVPGEKDAWWKSLAKAFN"
     gene            complement(1347..>1814)
                     /locus_tag="PLG52_12985"
     CDS             complement(1347..>1814)
                     /locus_tag="PLG52_12985"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HNB42413.1"
                     /translation="IGSLAPDFKLTATNGQSVSLGNFRGKSNVIVFFIRETTCPQCRT
                     HVAQLGKMYEQFKEAGTEVIVILGEGTDNAKDYANGIGLPFPILCDPDRAVYSLYELE
                     KYFLLFQRTASLVVDKDGIVRYLKRTTVPNVWLQESRELLGFILSLDEGQPVV"
BASE COUNT          452 a          424 c          453 g          485 t
ORIGIN      
        1 taagaattct tacgcactca gttatgattt tcttttgttg atctaaatat ttttgtaaat
       61 gaaggcgagt ttcatattct ttgccaagat tgtagggcgg tgaagttact actaatttga
      121 ctgtattgtc tggaattgtt tttagtaaat ctaagcaatc accttcaaat aaaatacaat
      181 ctgcatttgg gttaaatctg tttgatatct tcatgttatt gattatagat caattgttct
      241 gaaattgaga agtgttataa attaattaaa cgctttcgcc agactcttcc accatgcatc
      301 tttttcgccg ggcacgaaag gtgtgtaaag tgtgagtctg tctgcgatgc cgtcgtagcg
      361 tttcttcaag tcggtggcga gattttcctg cgtggtcatg agacaaaatt cgttcaacat
      421 ctcatcggtg ataagcatag gcatctcgtg ccattcgcct ttggcggcaa agccagaaag
      481 tttttcagcg gtctcgctcc agccgtataa gtccatcacc gagcgatagg aaggcgtcga
      541 agcatagaaa gcaatctgca tgcgggcgaa gccttcttct tcgggtgtcg tcgcaatgaa
      601 cggagtcata gataccgcaa ggtcgctgcg tttgcgtccg cttttttgaa gcccctcttc
      661 aatggcgggc aacaaaacct cattcatata gcgcggactg ttgaacggat gcgcatgaaa
      721 accttcgcac atctcacccg cgagccttgc caagccttgg ttgactcctg caatgtaaat
      781 cggaatattg gggttatcga tcttgccagg gttaaagaac ggtgacatga gcgtgatctt
      841 gtaatgttca ccgcggaagt ttaatttgct gccgttctgc cacgcatccc acaaggcgcg
      901 gatgacttga atttgctcgc gcaatttgcc ggtcactgat tcgggccaag tcatgccgaa
      961 acgacgctca atgtgcgcct tgacctgtgt gcccaagccc aaaatgaatc gtccgttcga
     1021 ctgagccgcc aagtcccatg ccgtgtaggc aaggttggca ggcgaacgcg caaacgacac
     1081 agcgatggcg gtccctatct ccatgttcgc agaatgttct gcgatgagcg tgcaaggcag
     1141 gaagggatcg tgttgcgtct cttgagtcca caaggcggcg aaaccagtct cttctgctgc
     1201 agtggcaatg gcaggaattt gcttcagttg gacgggagga atagcggcat ctagtttcat
     1261 agtggtaagt atacagtttg agaagagaat agggaatgct atccgcgtat aaaaataaaa
     1321 gccccgcatt tgcagggctt tgaaccttat acaaccggct gaccttcatc caaactgaga
     1381 atgaaaccca gtagctcacg actctcttgc agccaaacgt ttggcaccgt ggtgcgcttc
     1441 aaatagcgga cgatgccgtc tttatccaca acgagcgagg ctgtgcgctg aaaaagcaga
     1501 aaatattttt caagctcgta caagctatac accgcccgat cagggtcaca caaaattggg
     1561 aagggcaaac cgattccatt tgcatagtct ttggcgttgt ccgtgccttc gcccagaatg
     1621 acgatgacct ctgtgccagc ttctttgaac tgttcataca ttttgccaag ttgggcgaca
     1681 tgcgtgcggc actgtgggca ggtcgtttcg cggatgaaga acacgatcac attgctctta
     1741 ccgcggaaat ttcccagcga aacagactgc ccattggtgg ctgtcaactt gaagtctggt
     1801 gcgagcgagc cgat
//
LOCUS       DAOQNJ010000566         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_58085_length_1807_cov_7.772831, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000566 DAOQNJ010000000
VERSION     DAOQNJ010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58085_length_1807_cov_7.772831"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PLG52_12990"
     CDS             <1..568
                     /locus_tag="PLG52_12990"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNB42414.1"
                     /translation="AIKTLGVIQDVMNRDWLPVVRAWQLNERHYGSLQGLNKSEMAEK
                     FGEEQVKIWRRSYDVPPPALELTDERHPKFDRRYASLTPEQLPATESLKITLERVLPY
                     WTSTLAPEIKSGKRLLVVAHGNSIRALVKYLDNISESEITELNIPTGLPLVYELDKDL
                     KPIKNYYLGDAEEAAKKAAAVANQGKAK"
     gene            656..>1807
                     /locus_tag="PLG52_12995"
     CDS             656..>1807
                     /locus_tag="PLG52_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNB42415.1"
                     /translation="MPLSSRERVQAAIHHEIPDRVPIIIGASNATGIKMVTYRRLKKL
                     LNFEAPERYLYDFPELGTALIDEPILERLHVDVRGVLDREPQHILERNASREPDSMYV
                     NSWGGGVTKAGPDNWFPSYHPLAETHAIEDLEKYPWPDMNDPTRVAHVRAHAQQLHQE
                     NKYALMGTPWLAFPMERAYEMQRMDKFFLNMGRHPDFVVELLKKTGEMCKTLMGHFLD
                     ECGDVIDIIKIGDDLGMQSSLMLSPKMYRNYVKPIHADYISFIKSKTKAKVFFHTDGD
                     VFPLLPDFVEMGIDILNPIQTSAGKMSNLPELKKQFGKDLIFCGAIDTHRVLPFGTPE
                     EVRAEVKRIIDILGEGGGYMLASVHTILDDVPAENILAMVDAVEEFGWYK"
BASE COUNT          500 a          417 c          407 g          483 t
ORIGIN      
        1 cgccatcaaa accttgggcg ttattcaaga tgttatgaat cgcgattggt tgccagttgt
       61 ccgcgcttgg cagttgaacg aacgtcatta cggttcatta caaggtttga acaaatctga
      121 aatggctgaa aaatttggtg aagaacaagt gaaaatttgg cgacgttcat acgatgttcc
      181 gcctccagcc ttggaattga ccgacgaacg tcatccaaaa tttgatcgac gctacgcctc
      241 actgaccccc gaacagctgc cagccaccga gtcacttaag attacgctgg agcgtgttct
      301 cccttattgg acaagcacct tggcacctga gattaaatcc ggtaagcgct tacttgtcgt
      361 tgcccatgga aattccattc gtgcattggt gaaatacctt gataacattt ccgaaagcga
      421 aatcacggaa ttaaacatcc caaccggttt gcccttggta tatgaacttg ataaagattt
      481 gaaacccatc aaaaactatt atcttggcga tgctgaagaa gccgctaaga aagctgctgc
      541 ggtcgccaat cagggcaaag cgaagtaata cgtagtaagt aaacaattca actcagagga
      601 caatgatggt gaataaccag cattgtcctc ttttaatttg ccgaaggaga atgttatgcc
      661 actttcatct cgtgaacgtg ttcaagcagc cattcatcat gaaattccag atcgtgttcc
      721 cattattatt ggtgcaagca atgcgactgg gattaagatg gtgacatatc gccgtttgaa
      781 gaaactgctt aattttgaag cccccgaacg ttatttgtat gattttccag aacttgggac
      841 tgccctgatc gacgaaccta ttcttgaacg cttacacgtt gatgtgcgcg gtgtgttaga
      901 ccgtgagccg caacatatcc ttgagcgaaa cgccagccgc gagcccgact caatgtatgt
      961 caactcgtgg ggtgggggcg ttaccaaagc cggacccgat aattggtttc cctcttatca
     1021 tccactggct gaaacacatg caattgaaga cctcgaaaaa tacccctggc ccgacatgaa
     1081 cgaccctacc cgggttgcgc atgtacgagc tcatgcccag caacttcatc aagagaacaa
     1141 atacgctcta atgggcaccc cttggcttgc atttccaatg gaacgtgctt atgagatgca
     1201 gcgcatggat aagtttttcc tcaatatggg gcgacaccct gattttgttg tagaacttct
     1261 caagaaaacc ggcgaaatgt gtaaaaccct catgggacat tttcttgatg aatgtgggga
     1321 tgtgatcgat attatcaaaa ttggcgatga cttggggatg caatccagtc ttatgctttc
     1381 gcctaagatg tatcgcaact atgtaaagcc cattcatgca gattacattt cttttatcaa
     1441 gtcgaaaaca aaagccaaag tattctttca tactgatgga gatgttttcc cgcttttgcc
     1501 agattttgtc gaaatgggca ttgatatttt gaatccaatt caaacgtcag ccggaaaaat
     1561 gtccaactta ccagagctta aaaagcagtt tggcaaagac cttatcttct gcggtgcgat
     1621 tgacacccac cgcgtccttc cattcggaac accagaagaa gtccgtgccg aagtgaaacg
     1681 catcattgac atccttggcg aaggcggtgg ctatatgctg gcatccgtcc acactatttt
     1741 ggatgatgtc cctgctgaga atattcttgc catggtcgat gctgtagagg aatttggctg
     1801 gtataag
//
LOCUS       DAOQNJ010000567         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_58461_length_1801_cov_6.733104, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000567 DAOQNJ010000000
VERSION     DAOQNJ010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58461_length_1801_cov_6.733104"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1561
                     /locus_tag="PLG52_13000"
     CDS             182..1561
                     /locus_tag="PLG52_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HNB42416.1"
                     /translation="MKTKFFRPAWAVAFVLALAVLVVNVPGIAAALGRVFGYVPEVGL
                     VETTNGLRMLAEPVSSTRDGVTLTITNVFAYPDHVELQYEVSGIAPENDGWQSGESNT
                     NPTAFCGGVNPGSTFSKAGDARLRLPDGSILEREYLGKYPKNAFAMQPVYDTGLPADV
                     TTLTFVLDCIPQARLGAVPEHWELSFNLVDVPAGMVVGEPVTEVPQPVTTEEIPASPL
                     TEPVSVPKVTMTLERIVPMETNTIIYLHFNMENADPSLKSILPEGSYMNAYVIDSLGQ
                     KIPLRGNFALQPSDHKVGSAFEYVTESKPAEGALTVVVDQVIAYYAPLYTNPPLATPE
                     EMTFTFDAGENPQHGQEWDVNAPFTIAGFDFEVDYVKAVSYVDIEPLFDGSQGYEYGY
                     QFSIKADPSLGMIVDMDIMKDNCWLYEEKPLLYTQLCKDAYPSGQVPVTISRMSITIL
                     DDIQAEWKP"
     gene            complement(1572..>1801)
                     /locus_tag="PLG52_13005"
     CDS             complement(1572..>1801)
                     /locus_tag="PLG52_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42417.1"
                     /translation="STFSQAMVIPTSATMEGQLTREASTIIAGGARRASGASSTFEPQ
                     AAIMNGRKKSIKYLFTGFSFKIYTIDSDLLD"
BASE COUNT          459 a          426 c          445 g          471 t
ORIGIN      
        1 ccgtgaagaa atcggtgtat cgtttgcttg agaggctgaa aggacagatg gagtaaacat
       61 gaacgacttt aatccctctt ctgaatttga agaaaagatt cgtaaagcga tggctgcgcc
      121 gaaagcaaag cctgagtttg taaataaatt acgcagtgaa ctggcgagga ggccagtgaa
      181 aatgaaaaca aaatttttta gacctgcctg ggctgtggca ttcgtgcttg ctctggcagt
      241 gcttgtagta aatgtgccgg gcattgccgc agcattagga cgtgtgttcg gttatgtgcc
      301 cgaagttgga ttggtggaaa caacgaatgg cttgcgtatg ctggcagagc ctgtttcttc
      361 aactcgtgat ggcgttacat taaccatcac caatgtattt gcctacccgg accatgttga
      421 gttgcaatac gaagtaagcg gcatcgcgcc cgaaaatgat ggctggcagt ctggtgaaag
      481 caacacaaac cccaccgcat tttgtggtgg cgtgaatcct ggcagcacat tcagcaaagc
      541 aggtgatgca cgtttacgtc tgcctgatgg ctcaatcctt gagcgcgaat acttgggcaa
      601 gtatccgaag aatgcgtttg ccatgcagcc cgtttacgat actggccttc cagcagatgt
      661 gacaacgctt acctttgtgc tcgactgtat tccgcaagcc cgcctcggcg ctgtgcctga
      721 acattgggaa ctctctttca accttgtgga tgtccctgct gggatggtgg tgggtgaacc
      781 tgtgactgaa gtaccacagc ctgttacgac agaggaaatt cccgcctctc ccttaaccga
      841 acctgtctct gttcccaaag tgacgatgac ccttgagcgg atcgtcccaa tggaaaccaa
      901 cacgatcatc tatttacatt tcaacatgga gaatgccgac ccgtctttga agtccatcct
      961 gccagaggga agctacatga acgcctacgt gatcgactca ctgggacaga aaattcccct
     1021 gcgcggaaat tttgccttgc agccatctga ccataaagtt ggcagcgcct ttgaatatgt
     1081 gaccgaatcg aaaccagccg aaggtgcgct caccgtagta gtggatcaag tcattgctta
     1141 ttacgcgccg ctctacacca acccgccgct ggcaactcca gaagagatga ccttcacgtt
     1201 tgacgctggc gaaaatccgc aacacggtca ggagtgggat gtaaatgctc ctttcaccat
     1261 tgctggtttt gattttgagg tcgattatgt gaaggctgtt tcttatgtag acattgaacc
     1321 tcttttcgac ggctcccaag gttatgaata tggctaccag ttttccatca aagcagaccc
     1381 ttcgcttggc atgatcgttg acatggatat catgaaggat aactgctggt tgtacgaaga
     1441 aaagccgctg ctttataccc aattgtgcaa agatgcctat ccaagcgggc aggttccagt
     1501 cactattagc cgaatgtcca tcaccattct tgatgatatt caagctgagt ggaagcctta
     1561 ggacttttat atcaatcaag caaatcactg tcgatggtat aaattttgaa ggagaagcca
     1621 gtgaataaat atttgatact cttttttcta ccattcatga ttgctgcttg cggctcaaaa
     1681 gtcgatgaag cgccagatgc tctcctcgct ccgcccgcga ttattgtaga agcttcgcga
     1741 gttaattgtc cttccatagt agctgaggtt gggatgacca tagcctggga aaatgtcgat
     1801 a
//
LOCUS       DAOQNJ010000568         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_58682_length_1797_cov_9.303100, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000568 DAOQNJ010000000
VERSION     DAOQNJ010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58682_length_1797_cov_9.303100"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1797
                     /locus_tag="PLG52_13010"
     CDS             23..>1797
                     /locus_tag="PLG52_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB42418.1"
                     /translation="MTEQFTFGGEIVWRPSQAQVDGANLTAFMQIWKLPDFDALMQKS
                     THDVAWFTEAVLKFLDIRFYEPYSQVIDLKNGIQFPKWCVDGKMNIVHNCVEKWVENE
                     ADRKKIAIAFEGEDGITKTLTYEDVDKEINKTANALRTLGLGKGDAIGIFMPMTPEIV
                     IALLAIAKIGGIILPLFSGYGAGAIASRMVDADAKALFAADGAFRRGKAVEMKSVADE
                     AAEQIPTLKHMIVLKRTGQDVKMKAGRDHWWHELVDVQNDVCNTEQTSAEDPLMIIYT
                     SGTTGRPKGALHTHCGFPVKAAQDMAFGTDVHQNDVIYWMTDMGWMMGPWLVFGSLIL
                     GATIFLYDGAPDFPTPSRLWELVERHKINQMGVSPTLIRSLIPHGDEYFKKYDLSSLK
                     CFASTGEPWNPDPWMWLFANVGEKRIPIINYSGGTEISGGIVMGNMLLPLKPCAFSAP
                     CPGMDADVVDENGKSVRNAVGELVIKAPWIGMTRGFWKDQQRYLDTYWSRWENVWVHG
                     DFAAIDNDGLWYILGRSDDTIKIAGKRLGPAEVESILVRHEAIVEAAAIGVPHEVKGS
                     ELVLFAVTKPGVERSAVLRQELQEMV"
BASE COUNT          460 a          345 c          533 g          459 t
ORIGIN      
        1 ccaacttgac gaggagatgt tcatgaccga acagtttacg tttggcggtg agatcgtttg
       61 gcgaccttcc caagcgcagg tggatggggc gaatttgacg gcattcatgc agatctggaa
      121 actccctgat tttgatgcgc taatgcaaaa gtcaacgcat gatgttgcct ggttcactga
      181 ggcggtcttg aaatttcttg atattcggtt ttatgaaccg tattcacaag tgattgacct
      241 aaagaatgga attcagttcc caaagtggtg tgtagatggg aagatgaaca ttgtgcataa
      301 ttgcgttgaa aaatgggtgg agaatgaagc tgaccgtaaa aagatagcaa tcgcatttga
      361 aggggaagat ggaattacaa aaacactcac ctacgaagat gtagataaag agatcaataa
      421 gactgccaat gctttgcgaa cattaggatt gggcaaaggg gacgctattg gtatcttcat
      481 gccaatgacg ccagagatcg ttattgcctt attagcaatt gcgaagatcg gcggaatcat
      541 tctgcctttg ttttctggtt atggtgcggg cgctattgcc tcccgtatgg tagatgcgga
      601 tgcaaaggca ctatttgcag cggatggcgc cttccgacgg ggcaaggctg tggaaatgaa
      661 gtcggtggct gatgaagcgg cggagcaaat cccgaccctt aagcacatga tcgtcttgaa
      721 gcggactggt caagatgtga agatgaaagc aggaagagat cattggtggc atgagttggt
      781 cgatgtgcaa aacgatgttt gcaatactga acaaacctct gctgaagacc cgttgatgat
      841 tatttatacc tctggcacca ctggtagacc gaaaggtgcc ttgcatacac attgtggctt
      901 cccagttaag gcagcacagg atatggcgtt tggcacggat gttcatcaaa atgatgtcat
      961 ttactggatg acagacatgg gttggatgat gggtccatgg ctggtatttg ggagtttgat
     1021 tcttggcgcg acgatatttc tatatgatgg tgcgccagat ttcccaacac cgagccgact
     1081 ttgggagttg gtagaaagac acaaaatcaa tcaaatgggc gtttcgccaa cgttgatccg
     1141 ctcgttgatt ccgcatgggg acgagtattt taagaaatat gatctgtctt cgctcaaatg
     1201 cttcgcctca actggagagc cgtggaatcc ggacccgtgg atgtggttgt ttgcaaatgt
     1261 gggcgagaag agaattccga tcatcaacta ttctggcggg acggaaatct ctggcggcat
     1321 tgtaatgggc aatatgttgt tgccactcaa accttgtgca ttttcagctc cctgcccggg
     1381 catggatgca gatgttgtgg atgagaacgg taagtcggtt cggaatgcgg tgggcgagtt
     1441 ggtaataaaa gccccgtgga ttgggatgac gcgcggcttt tggaaagatc aacaacgcta
     1501 tttggatacg tattggagcc gatgggagaa tgtttgggta catggcgact ttgcggcaat
     1561 tgataatgac ggcttgtggt atatccttgg gaggtcagac gacacgatca aaattgccgg
     1621 caagcggctc ggacctgctg aggtggagtc gattcttgtg cggcacgagg cgattgtaga
     1681 ggcggcggca attggggtgc cacacgaggt caaggggagt gaactggtcc ttttcgcagt
     1741 gaccaagccc ggggtagagc gaagcgcggt tcttcgtcag gaattgcaag aaatggt
//
LOCUS       DAOQNJ010000569         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_58736_length_1796_cov_7.314187, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000569 DAOQNJ010000000
VERSION     DAOQNJ010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58736_length_1796_cov_7.314187"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PLG52_13015"
     CDS             <1..996
                     /locus_tag="PLG52_13015"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNB42419.1"
                     /translation="KVGDKATAEVDATRRHDIMRNHTATHLLHKALHEVLSDQAKQAG
                     SLVSPTHLRFDFNHPEAMTADQIERVEKMVNEAVAMDMPVVKVTKSLDEAKKEGAMAL
                     FGEKYGEVVRTISIMEGEKDKYSYELCGGTHLERTSDVGAFIIVSESSAAAGVRRIEA
                     VTGRGAYELIQKRFKTLKQTAGSLKTSLDEVPTKVDALQDEVSELKKELAALRTQAAL
                     STFNSLLANVQTAKDVNVLGAEIPNADVDTLRSLADKFREKYPKNGAAVFVSGSTVIA
                     TVTEDVVKRGVKAGDLITGIGGKGGGRPNLAQGSLSGDVAAALSRLGQVIEAKVK"
     gene            1047..>1796
                     /locus_tag="PLG52_13020"
     CDS             1047..>1796
                     /locus_tag="PLG52_13020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB42420.1"
                     /translation="MLNTSGVKRRQIYLTTFPVGALFVIVFVIVVREKLNFFSEVVGM
                     GSATMILLGSLVLYFQPLKVYTIETSFYFFMALFLPALAGALLTNPNTILNMSPALFA
                     ETINGLTLWVSIIFMGAFLSISPRAFKLLVILVFLVIFLIYVISAVALLQVYKWNFTY
                     LFHFLHSFFALGITMLLISRIGRIQLLHATTDKLTGLLNRYAGSAKLSEEFEYGAGNS
                     TDLAVILVDVDHFKEINDFHGHLVGDKVLKEF"
BASE COUNT          452 a          403 c          456 g          485 t
ORIGIN      
        1 aaagtgggtg acaaagccac agctgaagtg gacgcgaccc gccgccacga catcatgcgc
       61 aaccataccg ctacgcactt gctgcacaag gcgctgcacg aagtattgag cgaccaagcc
      121 aagcaggcag gttcacttgt ctcgccaact catttacgtt tcgacttcaa ccaccccgaa
      181 gccatgaccg ccgaccagat cgaacgcgtc gagaagatgg tcaacgaagc ggtggcaatg
      241 gatatgcccg tggtcaaggt gaccaagtca ctggacgaag ccaagaagga aggtgcgatg
      301 gcgctcttcg gcgagaaata cggtgaagtt gtgcgtacta tcagcattat ggaaggtgag
      361 aaagataagt attcctacga gttgtgcggt ggaactcacc ttgagcgcac gtcggatgtg
      421 ggcgcgttca tcatcgtcag cgaaagctcg gcggcagcgg gtgtgcgccg tatcgaagca
      481 gtcaccgggc gcggtgcgta tgagctcatc cagaagcgct tcaagaccct gaagcagacg
      541 gcaggctcgc tcaagacttc gcttgatgaa gtccctacca aagtggacgc actgcaagat
      601 gaagtctctg agcttaagaa agaacttgct gccttgcgca ctcaggctgc actttctaca
      661 ttcaactcac tccttgcgaa tgttcaaacc gccaaagatg tcaacgttct cggcgcggaa
      721 attcccaatg cggacgtgga tacgctccgc tcgctggcag ataagttccg cgagaagtat
      781 cccaagaatg gcgcggcagt tttcgtttcg ggcagcactg tcattgccac cgtgaccgaa
      841 gatgtggtca aacggggagt caaggctggg gacttgatca caggcatcgg cggcaaggga
      901 ggcggtcgcc ccaatctcgc tcagggtagt ttgagcggtg atgtcgctgc ggcacttagt
      961 cgcctcggac aggttattga agcaaaggtc aagtaaattt aaagggtggt tcgccaccct
     1021 ttttgcttac taggattgat ccgtctatgt taaatacgtc tggcgttaaa cggcgacaaa
     1081 tctatttaac aacttttccg gtaggagcac tctttgttat agtgtttgtt attgttgtcc
     1141 gtgaaaagtt aaacttcttc agcgaagtag tagggatggg ttcggcaaca atgattttgc
     1201 tgggttcctt ggtcttgtac tttcaaccgc taaaagtgta tacaattgaa acgtcattct
     1261 attttttcat ggcgttgttt ttacctgcac ttgcgggggc gttactaaca aatccaaata
     1321 ctattttaaa tatgtcaccg gcattatttg ccgagactat taacggtttg accctttggg
     1381 tatccataat ttttatgggt gcttttcttt cgatttcccc aagggcattt aaactgttag
     1441 tgatccttgt ttttttagtc atatttttga tttatgtgat ctcggcagtt gctttgctgc
     1501 aggtttataa atggaatttt acgtatttat tccattttct acactctttc tttgcattag
     1561 gaattacgat gcttttgatc tcgcggatcg gacgaataca actgctccac gcaacaacgg
     1621 ataaattaac cgggttgctt aatcgatatg caggttctgc taaactaagc gaagagttcg
     1681 aatatggcgc aggaaattca acagatcttg cggttatttt ggtagatgtt gatcatttca
     1741 aagaaataaa tgattttcat ggacatcttg taggagacaa agtcctgaaa gaattt
//
LOCUS       DAOQNJ010000570         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_58934_length_1793_cov_8.185846, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000570 DAOQNJ010000000
VERSION     DAOQNJ010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58934_length_1793_cov_8.185846"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLG52_13025"
     CDS             complement(<1..380)
                     /locus_tag="PLG52_13025"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB42421.1"
                     /translation="MDAKERNEKIELYGRGYDLLKAALAEVPAEAMLFKPEPKEWSVY
                     EVIIHIADSESNGALRARKLIVEPGGSIMGYSQPLWADVLKYHEHNLEDALEVTRLAR
                     KTTYQLLKRIPDIVFDTHWIKHPE"
     gene            complement(445..816)
                     /locus_tag="PLG52_13030"
     CDS             complement(445..816)
                     /locus_tag="PLG52_13030"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB42422.1"
                     /translation="MAKALGVGGIFFKSPDPKRLHAWYAKWLGMKTEEWGLAFYPKDY
                     PANSQTVWSAFDASTTYFDPSKTGFMFNLIVDDLEGALEQVQQGGAEVIGNIEKMEYG
                     SFGWFMDPDGNKVELWEPIKE"
     gene            complement(829..>1793)
                     /locus_tag="PLG52_13035"
     CDS             complement(829..>1793)
                     /locus_tag="PLG52_13035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNB42423.1"
                     /translation="SVSATAINKVCGSGLKAAMMSAQAIRAGDAELFIAGGFESMSRA
                     PYLVSGRMGELKFGNTQMTDSLLNDGLWDPFENWGMGNAAEFIADEYEVTRAAMDEFA
                     LRSHLKAVEAQEAGRFNAEIVPVQIPGRKGEVTTVAHDEGPRKDTSLESLAKLKPVFK
                     ADGKVTAGNSSSMNDGAAATIISSRGYAEKKGLKPLAKIVGYAQAAMEPKYLFAAPAF
                     AMPKLLKKIGWTLKDVDLIELNEAFAAQVLADGYALADQGWDWDKVNVNGGAIALGHP
                     LGASGARVLTTLIYALQNRGLKRGIASLCLGGGEAVAMAIELEN"
BASE COUNT          403 a          513 c          405 g          472 t
ORIGIN      
        1 tattcaggat gtttaatcca atgggtgtca aagacaatat caggaatacg tttcaaaagt
       61 tgataggtgg tcttgcgcgc caaacgtgtc acttcaagcg catcttcaag attgtgttca
      121 tggtatttca acacatccgc ccaaagcggc tggctgtagc ccatgatgct cccgcccggc
      181 tcgacgatca acttgcgcgc acgtaatgcg ccatttgatt cactgtccgc aatgtggatg
      241 atgacttcgt aaacgctcca ctctttcggt tctggtttga agagcattgc ctccgcaggg
      301 acttctgcca atgccgcttt taataagtca tatccgcgac cgtataactc tatcttttca
      361 ttgcgctctt tagcgtccat ttttattgtc tcctcataaa atacaaacgg agacgaacac
      421 cactgcccgt ctccgtttat ttggttattc tttaatgggc tcccataatt caactttatt
      481 cccatccggg tccataaacc atccgaatga gccatattcc attttctcga tattgccaat
      541 cacttccgcg ccaccttgct gaacttgctc aagtgcgcct tccaaatcat ccacgatcaa
      601 gttgaacatg aaacctgttt ttgatgggtc aaagtaagtt gtcgatgcat caaacgcgct
      661 ccatactgtc tgactgttgg ctggataatc ttttgggtaa aacgccagcc cccattcttc
      721 agttttcatc cccaaccact tggcatacca tgcgtggagt cgtttggggt caggtgactt
      781 aaaaaagatg ccgccaacgc ctaatgcttt cgccatgttg gtctactcct aattttccaa
      841 ttcaatcgcc atcgctaccg cttcgccgcc accaagacat aacgatgcaa tgcctcgttt
      901 tagtccgcgg ttttgcaaag cgtagatgag ggtggtcaac acgcgtgcgc cgctggctcc
      961 cagtgggtga ccgagcgcaa ttgccccgcc attcacgttg accttgtccc agtcccagcc
     1021 ctggtcggcg agagcgtaac cgtccgcgag gacttgggcg gcgaaagctt cattcaactc
     1081 gatcaggtca acatctttca acgtccagcc gatcttcttt aacaactttg gcatggcaaa
     1141 tgcaggtgct gcaaaaagat atttcggttc cattgctgcc tgggcatagc caacgatctt
     1201 tgcaagcggc ttgagccctt tcttttcagc atagccacgc gaagagataa tcgtcgctgc
     1261 cgcaccatcg ttcatcgacg atgaatttcc cgccgtcact tttccgtccg ctttgaagac
     1321 gggtttgagc ttggcaagcg actccagcga tgtatccttg cgcggacctt cgtcatgcgc
     1381 cactgtggtc acttcccctt tgcgccccgg aatttggacg ggaacgatct cagcgttgaa
     1441 acgtcccgcc tcttgagcct ctacagcttt gaggtgagag cgaagcgcga attcatccat
     1501 tgcggcacgg gtcacttcgt attcatccgc gataaattct gcggcgttgc ccatgcccca
     1561 attctcgaac ggatcccaca aaccatcgtt gagcagagag tcggtcattt gggtgttgcc
     1621 aaatttaagt tcgcccatgc gcccactgac gaggtacgga gcgcgactca ttgactcaaa
     1681 accgccagcg atgaaaagct ccgcatcacc cgcccgaatg gcttgagcag acatcatcgc
     1741 cgctttcaag cccgagccgc aaaccttgtt gatggcagtc gcactgacag atg
//
LOCUS       DAOQNJ010000571         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_59370_length_1786_cov_10.123050, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000571 DAOQNJ010000000
VERSION     DAOQNJ010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59370_length_1786_cov_10.123050"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLG52_13040"
     CDS             complement(<1..675)
                     /locus_tag="PLG52_13040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNB42424.1"
                     /translation="MNWLEVSLTVNGELAESVADVLARFAPNGVMTEQGVRFVNDEDE
                     GTATGPITVRAYLEVNDQLEETRQKIEESLYYLGMITPVPTPTYKQIADQNWMEAWKQ
                     HYKPILIGEGLLILPAWLDNPDPKRIPIKIDPGMAFGTGTHPTTQLCLELMERSVNSN
                     WRLVIDVGCGSGILSIAALKRGAEKVLGVDIDIESVKNSRENADLNGIGEELTLGQGS
                     VTEVLAG"
     gene            complement(679..1158)
                     /locus_tag="PLG52_13045"
     CDS             complement(679..1158)
                     /locus_tag="PLG52_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB42425.1"
                     /translation="MESRKYPVHIVAAGGFVINSENQVLMLKSPRYDDWEFPGGQIEE
                     GEIIPKGLEREVLEETGIVVRVKNLIGIYSNIGNPPIVNMDFLCEYISGEPKTSNESI
                     DVKWVDRNEVLNLVERKVIRTRLQNMLEFSGEINYLAYSVNPNIKDLSYQEFENRKI"
     gene            complement(1161..1544)
                     /gene="crcB"
                     /locus_tag="PLG52_13050"
     CDS             complement(1161..1544)
                     /gene="crcB"
                     /locus_tag="PLG52_13050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNB42426.1"
                     /translation="MTNILLVGAGGFIGSVLRYLVSGYVQQSANRMDFPFGTLAVNII
                     GCFIIGLLAQFGDKYGTFSNESRAFLVIGVLGGFTTFSSFGNDTVNLIRQDFVMNAVA
                     NVGANVIIGLLAVWLGRTVGQLIWR"
BASE COUNT          440 a          483 c          379 g          484 t
ORIGIN      
        1 ccccgccagc acctcggtca cggaaccctg cccgagggtc aactcctccc ctattccatt
       61 caagtctgca ttctcacggg agttttttac ggactcgatg tcaatgtcaa cccccaatac
      121 cttttctgcg ccacgtttca acgccgcaat tgaaagaata cctgagccac accccacatc
      181 aattaccaat ctccaattac tatttaccga tctctccatc aactccaaac ataattgcgt
      241 cgtcggatga gtacctgtgc caaacgccat gccggggtcg atcttgatcg ggattcgttt
      301 tgggtcgggg ttgtccaacc aggcagggag gatgaggagc ccttcaccga tgagaatcgg
      361 cttgtagtgt tgcttccagg cttccatcca gttttgatcc gcgatctgct tgtaggtggg
      421 agtgggtacc ggtgtgatca tccccaaata atagagcgat tcctctattt tctgtctcgt
      481 ctcttcgagt tgatcgttga cctccaaata tgctcggacg gtgatgggac ccgtggcagt
      541 gccctcgtct tcatcgttca caaagcggac accctgctcg gtcatcacgc cgttgggcgc
      601 aaaacgtgcc agcacatcgg caacagattc ggcgagttcg ccgttgacgg tcaaagaaac
      661 ttcaagccag ttcatgttct atattttcct attctcgaat tcttgataac tcaggtcttt
      721 gatatttggg ttcacactat aggcgagata attaatttcg ccagaaaatt caagcatatt
      781 ttgcaatcgt gtgcgaatga cttttcgctc taccaagttt aacacttcat ttcgatcaac
      841 ccattttacg tcaatgcttt cgttgctggt tttgggttca cccgaaatat attcgcacag
      901 aaaatccatg ttgacgattg gcgggttacc aatgttcgaa tagatcccta tgaggttttt
      961 aactctgact acaattcctg tttcttccaa cacttcccgt tctaaacctt taggaatgat
     1021 ctcgccttct tcaatctgcc cgccaggaaa ttcccaatcg tcgtagcgtg gacttttcag
     1081 catcaaaacc tgattctcgc tgttgatgac aaatccgcct gccgccacga tatgaactgg
     1141 atactttcta ctttccacac ttatctccag attaattgtc caacggttcg acccagccaa
     1201 accgcgagta gtccgattat cacattcgcg ccgacatttg cgacggcgtt catgacaaag
     1261 tcttgccgta tcaagttgac cgtgtcattg ccaaaggatg aaaaggtggt gaagccgccc
     1321 aagacgccga tcaccagaaa ggcgcgtgac tcatttgaga acgtgccgta tttatcgcca
     1381 aactgcgcca gcaaaccaat gataaaacaa ccgatgatat tgaccgccaa cgtaccgaag
     1441 ggaaaatcca ttcgattggc agattgctgc acatatccgc tgaccagata acgcaagacc
     1501 gagccgataa atccgcccgc gccgacaaga agaatgtttg tcaaatttgc tcccaccaca
     1561 gagagcacgg ggcacacaga gaattaaaga ggttttctcc gtgtgctttg tgtgctctgt
     1621 ggttaattct tttcaagttc tttcgctgcc cagcggatca aatccttctc cgcgaacagc
     1681 atgatgggca agccattcac atcgaagtat ttcatctctg aaatctcatg tgacggtttg
     1741 aactcgccgc ccgttatttt acacagatat gtgattccca aatgcg
//
LOCUS       DAOQNJ010000572         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_59621_length_1782_cov_9.434279, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000572 DAOQNJ010000000
VERSION     DAOQNJ010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59621_length_1782_cov_9.434279"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            396..965
                     /locus_tag="PLG52_13055"
     CDS             396..965
                     /locus_tag="PLG52_13055"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HNB42427.1"
                     /translation="MSSLDKAVQTQLENIQKKTGKSLAELAAIAQKSGLTKHGELRDM
                     FKEKLGLGHGDANALVHAVFKSDGTRAAEGKSSDAVLDEIYVGAKASQRPIHEALMKQ
                     INKFGEFEVVPKKGYVSLRRKKQFAMIGPKTNTRFEVGINAKEMKKNPRLVEQPKGSM
                     CNYIVALTDASEVDAELIGWIKLAYEGAD"
     gene            965..1423
                     /locus_tag="PLG52_13060"
     CDS             965..1423
                     /locus_tag="PLG52_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB42428.1"
                     /translation="MSEDFRKNLLNFMTETFESPMGIYLDKNTALFQTLETVSAAEAS
                     IPVGGKCATLAAQVAHVTYYLDVLERYVVHNDTSPADWGEVWRTVSGVTTQEWETSKA
                     KLRSAYQRMVELFKTNPVWNETTMGGAMAIVVHSAYHLGEIRQALCTLKK"
     gene            1442..>1782
                     /locus_tag="PLG52_13065"
     CDS             1442..>1782
                     /locus_tag="PLG52_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42429.1"
                     /translation="MSTNHAHYLVEEYAFHNWLVHHILEGIDHEESLLQLPFESNSTN
                     WILGHIVTNRSHVLEVLGVTHLWQEEVRELYHTGTHNIGHGDKAMRLETLLKYMDDST
                     RMIRRALEPVS"
BASE COUNT          499 a          362 c          489 g          432 t
ORIGIN      
        1 acaggaatct ctacaagggt gggggtgggt ggaacaggtg tgggggtagg aacagcttga
       61 gtggcagtag ccggaatcgg cgtgggggga ggcggcgtac ttttcgttgt gcatcccacc
      121 aaggtgataa tgagtcctgc aagcaggacg aggcggttca tttttcctcc ttttgtcatt
      181 cgtttcaact ctccatcggc gttggagagg caaggcaatt cagggttgaa tcacctgcat
      241 gttctataga agtgtaacag ggaagctaaa gttcagatgc gctcatagaa tggaattagt
      301 actttgtggg gatatatgat tgcaaaatta gaaaatttgt tctataatta taccattgtg
      361 taactcagct gttttattca aaaggagatg agagtatgag tagtcttgat aaagccgttc
      421 aaacacaact ggaaaacatc caaaagaaga ccggtaaatc gctggcggag ttggcagcca
      481 ttgctcaaaa gagtgggctg accaagcatg gcgaactgcg cgatatgttc aaggagaaac
      541 tggggcttgg gcatggcgac gcaaatgcac tggtgcatgc ggttttcaag tctgatggta
      601 cacgcgctgc cgaagggaaa agctctgatg ctgttttaga tgaaatttac gtcggtgcca
      661 aagcctcgca gcgccccatc catgaggcgt tgatgaaaca gatcaacaag ttcggagagt
      721 ttgaggttgt gccgaagaag ggatatgtaa gtttgcggcg caagaagcaa tttgcgatga
      781 tcggtcctaa aaccaatact cgttttgaag tgggtattaa tgccaaagag atgaagaaaa
      841 atccgcgttt ggtagaacaa cccaaaggca gtatgtgcaa ttacatcgtt gccctgacgg
      901 atgccagcga agtggatgct gaactgattg gctggatcaa acttgcatat gaaggagctg
      961 attaatgtcg gaagatttta gaaaaaatct tttgaacttt atgaccgaga cctttgaaag
     1021 ccccatggga atttaccttg ataagaacac ggctctgttt cagacgctcg aaacggtctc
     1081 tgcagcggag gcttcgattc ccgttggcgg gaagtgcgcc acgctcgcgg cacaggttgc
     1141 gcatgtgacc tattatcttg atgtgctgga gcgttatgta gttcacaacg atacatcgcc
     1201 tgccgattgg ggcgaggttt ggcgcacggt gagcggcgtc acgacgcaag aatgggaaac
     1261 gagtaaagcc aaattgcgaa gtgcctatca acgcatggtg gagttattca agacaaaccc
     1321 agtttggaac gaaaccacga tgggcggagc catggcaatt gttgttcaca gtgcctatca
     1381 cctaggagag atacgtcagg cgctttgcac gttgaaaaaa tagagaaaag gaaacataca
     1441 catgtcgacc aatcatgccc attacctagt tgaagaatat gcctttcata actggttggt
     1501 tcatcacatc cttgaaggga tcgatcacga agaaagtttg ttacaactgc cctttgaatc
     1561 caactcaaca aattggattc tcggtcatat cgttaccaat cgcagccatg tactggaagt
     1621 attgggagtc acacacctct ggcaggaaga agtccgcgag ctgtatcaca ccggcactca
     1681 taatattggt catggcgata aagccatgcg cttggaaact cttctgaagt acatggatga
     1741 tagtacgcgc atgattcgca gggcgctgga acctgtcagc ga
//
LOCUS       DAOQNJ010000573         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_59915_length_1777_cov_7.746806, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000573 DAOQNJ010000000
VERSION     DAOQNJ010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59915_length_1777_cov_7.746806"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1777)
                     /locus_tag="PLG52_13070"
     CDS             complement(<1..>1777)
                     /locus_tag="PLG52_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB42430.1"
                     /translation="IAHEGVRPWQILAVTFTNKAAKEMDERVKAILNEQATEGMMLGT
                     FHSICARILRREAEHLPVAQNFVIFDADDQQSLVKSIIREMNLNEKLYRANSVHASIS
                     RAKNELILPDDYPINTYRDEVVKRVYTEYQKRLIASNAVDFDDILVYATQLMENNPTV
                     REKYAQRFRHVLVDEFQDTNLAQYALVKNLASYHKNIFCVGDPDQCFPAGVKIQTPHG
                     QKKIEDIKRGDEVTVASGRGAAMTSKVTHAGKRAFKGNLVRAKTKTGHIIRATPNHIM
                     FARLGMKPGLHYVYLMYRKDKGYRVGIASHARSDGSSLKVGLQVRSNQENAHKMWILK
                     VCSDRNEAHYWEAYYSTAYGIPTTVFHVRGRRMRMSQEYIDRLFAEINTDENANQLLT
                     DLGMDFKYPHYIPQGTYRNIINLRYFGDGRITEESPWHAHRVDLWSSDEKLGKQLKAQ
                     RYNPRIKSRNSWRIGVNRLQYDDIQDVAQKLSEAANDAEIVTGAFLVDKGNSPLANKF
                     SLMPASHLHPSMIVAIEKNGAIIEDEIVEVTHEPYSGTVYDFEVENLHNYIANGVVVH
                     NSIYAWRGADYRNIQRFEQDYPDSQT"
BASE COUNT          400 a          407 c          452 g          518 t
ORIGIN      
        1 gtttgcgagt cgggatagtc ttgctcaaag cgttgaatgt tgcgatagtc cgcgccgcgc
       61 caggcgtaga tggagttgtg aactacaaca ccattagcaa tatagttgtg cagattttcc
      121 acttcaaaat cgtagacagt tccgctgtaa ggctcatggg ttacttctac aatttcgtct
      181 tcaatgattg ccccattctt ttcaatggca acgatcatgg atgggtgtaa atgactggcg
      241 ggcatgaggc tgaatttatt ggcaagcggt gaattgcctt tgtctacaag gaacgcgccc
      301 gtaacaattt ctgcgtcgtt tgcggcttcg ctgagttttt gggcaacatc ctgaatgtca
      361 tcatattgca atcggttgac gccaatgcgc caactgttac gacttttaat gcgagggtta
      421 tacctttgtg cttttaattg cttgccgagt ttctcgtcac tactccataa atccacccgg
      481 tgggcgtgcc agggagattc ttcggtaatt cttccatctc caaagtaacg caggttgatg
      541 atatttctat acgttccttg tggaatgtaa tgcggatatt tgaagtccat gccaaggtcg
      601 gttagtaatt gattggcgtt ttcgtctgta ttaatttcag caaacaggcg gtcaatatat
      661 tcctgcgaca tacgcatcct gcgcccgcga acgtgaaaca cagttgtggg gatgccgtag
      721 gcagttgagt agtaggcttc ccagtaatgg gcttcattgc ggtctgagca aactttcaaa
      781 atccacatct tgtgggcatt ttcctgattg ctgcgaactt gtaagccaac ttttaatgaa
      841 gaaccatcgc tgcgggcgtg agatgcaatg ccaacgcgat aacccttatc ttttcgatac
      901 atcaggtaga cataatgcaa gccgggtttc ataccgagcc tggcaaacat gatgtggttg
      961 ggcgttgctc ggatgatgtg tccagttttt gtttttgcac ggactaaatt gcctttgaat
     1021 gcccgcttcc cggcgtgtgt cactttggag gtcattgctg ccccacgtcc actggcgaca
     1081 gtgacttcat cccctcgttt gatgtcttca attttctttt gcccgtgtgg ggtttgaatt
     1141 tttacgcccg ctggaaaaca ttgatcgggg tcacccacgc agaaaatatt cttatgatag
     1201 gaagcaagat tcttcaccaa agcatactgc gcgaggttgg tatcttgaaa ttcatctact
     1261 aaaacgtgcc gaaagcgctg agcatatttt tcgcgcacag ttgggttgtt ttccatcaac
     1321 tgcgtggcgt acacgaggat gtcgtcaaag tccacggcgt tgctggcgat gaggcgcttt
     1381 tgatattcgg tatacacacg cttcactact tcatcgcggt aggtgttgat ggggtaatca
     1441 tccggcagga tcaactcgtt tttagcacgc gaaatcgaag catggacact gtttgcgcga
     1501 tacaacttct cattcaagtt catctcgcga atgatggact tgaccaaact ctgctggtcg
     1561 tcggcatcga agatgacaaa gttttgcgcc acgggcaggt gctcggcttc gcgtcgcaaa
     1621 atccgcgcac agatggagtg gaaggtgccg agcatcatgc cttcagtcgc ctgttcgttc
     1681 aaaattgctt tgacacgttc gtccatttct ttggcggctt tgttggtgaa ggtgacggca
     1741 aggatttgcc acgggcggac gccttcgtgg gcaataa
//
LOCUS       DAOQNJ010000574         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_60582_length_1766_cov_6.688486, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000574 DAOQNJ010000000
VERSION     DAOQNJ010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60582_length_1766_cov_6.688486"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1766
                     /locus_tag="PLG52_13075"
     CDS             <1..>1766
                     /locus_tag="PLG52_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42431.1"
                     /translation="FHNPDQPPIFLTLETAQYYMAHLVRPLIEERGYFDSVTINRNRL
                     YSQIIDNLNKSAIVGIPYTEIGARLDAAWFGDPAQRRVYADAQECVTLFRQYCLEHNL
                     LDFSLQMEVFKNHLWQNDQARSYLIQTYRHLIYDNVEEDVSSSHDILDQWVPEFESAL
                     LLYDVGAGYKKFLGGDPASGYALSGRCDELFELNESFVSSPNVIQLSNALVSAINGQG
                     EIEADSTQPAMEFIMTHFHPEMLDEVVTKIRLLLEAGTPPSEVVILAPYLSDSLRFSI
                     SNRLDSAGIPWRSNRPSRSLRDEPASKALLTLSALAHPQWNVHPSKFDVAHALMFALG
                     TDLIRSQLLTDIVYGQRELRLSSFDQINPETQERITYALGARYATLREWIETYRNEAP
                     PPLDFFLRRLFGEVLSQPDFGFHTNLDAVRIAASLVESIKKFRNAMEPLQTLNDFDLG
                     MEYITMLQDGVIAAQYLESWRSEDKEAVLVAPAYTFLMMNRPAAFQFWLDPGSDGWSQ
                     RVSQPLTHPYILSRYWDSSSGRVWLDSDEVEMEIETLSRLVGGLLARCREKVFLALSD
                     LGAAGFEQRGALLRAFQKVLQR"
BASE COUNT          411 a          475 c          386 g          494 t
ORIGIN      
        1 tttcacaacc cagaccagcc ccccatcttc cttacattag aaaccgccca gtattacatg
       61 gcgcatttgg tacgtccatt aattgaagaa cgcggctact ttgactctgt caccatcaac
      121 cgcaaccgtc tctattccca gattatcgat aacctcaata aatctgccat cgttggcatt
      181 ccatataccg aaattggcgc tcgcttagat gccgcctggt tcggtgaccc tgcccagcgc
      241 cgggtatatg cagatgcgca agaatgtgtc accttgttcc gtcaatattg ccttgaacat
      301 aatttgctag atttctcact tcaaatggaa gttttcaaaa atcatctctg gcaaaatgac
      361 caagctcgtt cgtatcttat tcaaacctat cgccatctta tttatgataa tgtcgaagaa
      421 gatgtctcgt cttctcatga tattctcgac caatgggtac ctgaatttga gtccgcgctt
      481 ttgctttacg acgtaggtgc aggctacaaa aaatttctgg gtggagaccc agccagcggc
      541 tacgccctga gcggcagatg cgatgaatta tttgagttaa acgagtcatt tgtctcgtcg
      601 ccaaatgtaa ttcaactctc aaacgcactt gtctcagcta tcaatggtca aggtgaaatt
      661 gaagcggata gcacccaacc tgcgatggaa ttcatcatga cacacttcca tcctgagatg
      721 ttggatgaag ttgtcacaaa aattcgcctg ttgcttgaag ccggcacccc gccttctgaa
      781 gtagtaattc ttgcgccata tctatcagac tcgctacgat tctcgatttc gaatcgttta
      841 gattctgctg ggattccctg gcgcagcaat cgtccatctc gttctttgcg cgatgaaccc
      901 gccagcaaag ccttgctcac tttatcggct cttgctcacc cacagtggaa tgttcatcca
      961 tcaaagtttg acgttgccca tgctttgatg tttgcccttg gcacagacct catccgctcg
     1021 cagttgctca ccgatattgt gtatggtcaa cgtgagttaa ggctttcaag ttttgaccag
     1081 atcaaccctg aaacccaaga acgtattacc tacgctttgg gggctcgtta cgcaacattg
     1141 cgcgagtgga ttgaaactta tcgcaacgaa gcgccacctc cgctcgattt tttccttcgc
     1201 cgcttatttg gcgaagtgct ctcgcaacct gattttggtt ttcataccaa cctcgatgcg
     1261 gttcgtatcg ccgccagcct tgtcgaatcg atcaaaaagt tccgcaacgc catggagccg
     1321 cttcaaactc taaacgactt tgaccttggc atggaataca tcaccatgct gcaagatggg
     1381 gtcattgcag cccaatatct cgaatcatgg cgcagtgaag ataaagaagc cgttttggtt
     1441 gccccggcat ataccttcct aatgatgaac cgccccgccg ccttccaatt ctggctggac
     1501 cctggttctg atggctggtc acagcgcgtg tcacagccgc tcacccatcc ctatatcctt
     1561 tcgcgctatt gggattcgtc ttctgggcgc gtttggcttg actcggatga agtcgaaatg
     1621 gaaatcgaga ccctatcccg cctcgttggc ggtttgctcg cccgctgtcg tgaaaaggta
     1681 tttttggcat tgtctgatct tggtgccgca gggtttgaac aacgtggcgc gttattacgg
     1741 gcatttcaga aggtgttaca acgaca
//
LOCUS       DAOQNJ010000575         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_60647_length_1765_cov_10.833918, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000575 DAOQNJ010000000
VERSION     DAOQNJ010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60647_length_1765_cov_10.833918"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLG52_13080"
     CDS             complement(<1..655)
                     /locus_tag="PLG52_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HNB42432.1"
                     /translation="MIIQTAFEGKPNFAIEQPDHTRTCGQLARAFGNADFAQPTPRDL
                     VVYIVEHHDEGWAPIDALREQSPTTGLPHHLTQTPIQYLIQTSKGSPEFNEKHHAYCG
                     LLSSMHTYGLFNGRYGLSDFIFIDKISPEHKASADAMLAYELARQARLKADLGADSTS
                     QAWIEEKALFNNYKLLQFFDTLALYFHTTHTEGRKEGKFLNVPDGKGTDHTISITPRE
                     "
     gene            complement(722..1177)
                     /locus_tag="PLG52_13085"
     CDS             complement(722..1177)
                     /locus_tag="PLG52_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42433.1"
                     /translation="MTKTYGDPKSTIPYQCFMGRWEGLCKTFSPKGDFIESAAVHMDV
                     YWNEIGKSWHLMEDFENLYEVGKTVFHSDISTDGKFCWATSEQLSLNGSELTPYNYVF
                     TIDSKISKTLVHNNHYFIDPNNRRIITHKLREGKTHIFQIQDFVRIQQP"
     gene            complement(1179..>1765)
                     /locus_tag="PLG52_13090"
     CDS             complement(1179..>1765)
                     /locus_tag="PLG52_13090"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB42434.1"
                     /translation="SRITVRQALSDLVYEGLLIRERGRGTFVAPPKMTHSPEERKSLT
                     EFMTEKGIKPGWQVIEKGFVEANKEVASNLKLPHKSRVYRIRRLRLAADEAIGHHTAY
                     LPEAVVGQIDEAGLLDGGSLNYIAHIPQMATSHATRSIEAVAASDLDVKLLHMSLGSP
                     VLMIQRVVLSAAGEPLEFLQARYRGDRFKYQIGD"
BASE COUNT          396 a          417 c          487 g          465 t
ORIGIN      
        1 cttctcgcgg ggtgatgctg atggtgtgat ctgtcccttt gccatcgggc acgttcagga
       61 atttaccttc cttgcgtcct tcggtgtgtg tagtgtggaa atacagcgcc agcgtgtcga
      121 agaactgcag gagtttgtag ttgttgaaca aggctttttc ttcgatccaa gcctgggagg
      181 tggagtccgc tccgaggtcg gctttgaggc gggcttgacg ggctaactcg taggcaagca
      241 tcgcatccgc tgaggctttg tgctcaggtg aaatcttgtc gatgaaaata aaatccgaaa
      301 gaccatagcg tccgttgaac aagccatagg tgtgcatgga cgagagcaag ccgcaatagg
      361 cgtgatgttt ttcgttgaac tcgggcgaac ccttgctggt ttgaatgagg tattgaatgg
      421 gagtctgcgt gaggtgatgc ggcaagcctg tggttggcga ttgctctcgc aacgcatcga
      481 tgggcgccca accttcgtcg tgatgttcaa cgatgtagac aacgagatcg cgcggtgtgg
      541 gttgagcaaa atccgcgttg ccaaatgcac gcgcgagttg accacaggtg cgcgtatggt
      601 cgggctgttc aatggcaaag tttggtttgc cttcaaaggc ggtctggatg atcatggctc
      661 tcctctttga caatagacga tgaaccattg accatggtct gtcgtctatc gtccatcgtc
      721 tctaaggttg ctgtattctc acaaaatcct gaatttgaaa aatatgcgtc ttgccctcgc
      781 gtaatttatg ggtgatgatg cggcggttgt tggggtcaat aaaataatga ttgttgtgaa
      841 caagagtctt ggagattttg ctatcaatgg taaagacgta gttatacgga gtgagttcag
      901 agccattcag actcaactgc tcactggttg cccaacagaa ttttccgtca gtggagatgt
      961 cagaatgaaa aacggtcttg cccacttcgt acagattttc aaagtcttcc atcaagtgcc
     1021 aactcttacc gatctcgttc caatacacat ccatgtgaac agcggcggat tcgataaagt
     1081 cgcctttggg gctgaaggtt ttgcacaagc cttcccatcg tcccatgaag cattgatatg
     1141 gaatggttga tttcgggtcg ccgtaggtct ttgtcatgtt aatctccaat ttgatatttg
     1201 aatcggtcgc cgcgataacg ggcttgcaag aactcaagtg gttcgccagc cgcgctcaac
     1261 accacacgtt gaatcatcag tacaggtgaa cccaaagaca tgtgaagcag cttcacgtcg
     1321 aggtctgagg cggcaacggc ttcgatggaa cgagtcgcat gcgatgtcgc catctgcggg
     1381 atgtgggcga tgtagttgag cgatccccca tccaacaatc ccgcctcatc gatctgacct
     1441 acaacggctt cgggcaaata agcggtatga tgtccgatgg cttcatctgc cgcgaggcgc
     1501 aaacggcgaa tgcgatagac acgcgattta tgcggcaatt tcaaattcga tgccacttct
     1561 ttattcgctt ccacaaatcc tttttcgatg acctgccagc ctggcttgat gcccttttcg
     1621 gtcatgaact cggtcaggct tttgcgttct tcggggctgt gagtcatctt gggcggtgcg
     1681 acgaaggtgc cacgtccgcg ctcgcggatg agcaagcctt catagacaag gtcgctcaac
     1741 gcctgacgaa ctgtgatgcg gctca
//
LOCUS       DAOQNJ010000576         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_60808_length_1762_cov_12.389572, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000576 DAOQNJ010000000
VERSION     DAOQNJ010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60808_length_1762_cov_12.389572"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..942
                     /locus_tag="PLG52_13095"
     CDS             307..942
                     /locus_tag="PLG52_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB42435.1"
                     /translation="MKKTQPTSPRVQRNREVATSAILDTARAIMREEGVAALSMHELA
                     RRMGMRAPSLYNYFNSKMEIYDALFCLGFNLYNTQLQEALKESKTPHDEIRIAIECYM
                     SFALENPELYQLCFERPVPGFVPSKESLELSFGILNNTYTRIEKFKKQFDTKLTAQQI
                     VDMVIAVAHGLTALHLANEPQLPIGQGRFGSLIPTFVRFFETSMPAKGENK"
     gene            939..>1762
                     /locus_tag="PLG52_13100"
     CDS             939..>1762
                     /locus_tag="PLG52_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HNB42436.1"
                     /translation="MTAILSTSNIARAEAIPMTHADEAHRLMTEALERLLSLLESLHA
                     DDWSKPTACTAWNVHDMVAHQAGGYLSGISLREMVRQYSQVPKKGQLPEDAINELQVG
                     ERKNKSDSELIAELKQVGAIAIHNWAYGFNAIKWIAMPHPVGGFMSIRHLMWVIHSRD
                     TWMHRLDICRATNRPFEQTREHDGRIVELVVLDTAKKIKKRLNGRAINLLLTGVAGGT
                     WQIGNGSPVAELEMDALDFNIFVSGRYSFEEGMQRAKISGDTKLVESAFKNFLILY"
BASE COUNT          498 a          439 c          380 g          445 t
ORIGIN      
        1 ccaaaaactt caattggtcc agtgctgcca gactgcataa atgtgtaata ggcatatccg
       61 ccaatccctg ccgccaaaag acagagacag cataacagca caacgattcc tacgatgatg
      121 cttgtcatgc cgccagatga tttattttca agattatcca tgaatgttcc tctctcctaa
      181 aaggttgtcc gatgataccg ccatccgcta aaaaacggat taggacttta ggggtaatgg
      241 tgagccaatc tcttgacaaa ggcaattttt ctaatataat tagaaaaaac taacaatatt
      301 agaaaaatga agaaaacaca gcccacctcc ccccgtgttc aacggaaccg tgaagtcgcc
      361 acctctgcta ttttggatac ggcccgcgct atcatgcgtg aagaaggggt tgctgccctc
      421 tccatgcatg agttggcgcg tcgtatgggc atgcgcgctc cgtcactcta caactacttc
      481 aacagcaaaa tggaaatcta tgatgccctc ttctgtttgg ggtttaacct gtataacact
      541 caacttcaag aagcgctcaa agaatcaaag actccacatg acgaaattcg gattgccatc
      601 gaatgttaca tgtctttcgc cttagaaaac cctgagctgt accaactatg ttttgagcgc
      661 ccggttcccg gttttgtgcc ctccaaagaa agcctcgaac tcagttttgg cattcttaac
      721 aacacctata cacgcataga aaaattcaag aaacaattcg atacaaaact caccgctcaa
      781 cagatcgtgg atatggtcat tgcagtcgct cacggtctca cagctttgca tcttgccaac
      841 gaaccgcaac ttcctattgg gcaaggacgt ttcggttctc ttatccctac tttcgtgcgt
      901 ttctttgaaa cgtctatgcc cgccaaagga gaaaacaaat gaccgccatc ctttcgacat
      961 caaacatcgc ccgcgctgaa gccatcccca tgacccatgc ggatgaagcc catcggctca
     1021 tgaccgaagc cttggagcgg cttttgagcc tgctcgagtc tctccacgct gacgattggt
     1081 ctaaacctac agcctgcaca gcatggaatg ttcacgacat ggtagcacac caggcaggtg
     1141 gatacttaag tggcataagt ttacgagaga tggttaggca atattcccaa gttcccaaga
     1201 aaggacaatt gcccgaagat gccatcaatg agttacaggt gggagaacgc aagaataaat
     1261 ctgattctga attgatcgct gaactaaaac aagtcggtgc tattgccatt cataactggg
     1321 catatggttt caatgctatc aaatggatcg ccatgccgca cccagttggc ggtttcatgt
     1381 cgattcgtca cctaatgtgg gtcattcaca gccgcgatac atggatgcat cgtctggata
     1441 tctgccgcgc taccaaccgt ccttttgagc aaacgcgtga acatgatggg cgtattgtgg
     1501 agcttgtcgt tttagataca gcgaaaaaaa taaaaaaacg tctcaatggt cgtgctatta
     1561 atttactgtt gacaggcgtc gctggcggaa catggcaaat tggaaatggt tctcccgttg
     1621 cagagctcga aatggacgca ctggatttca acatattcgt ctcaggtcgg tactcgtttg
     1681 aagaaggcat gcaacgcgcg aaaatctcag gcgacacaaa actagttgag tccgctttca
     1741 aaaatttctt gatcttgtat ta
//
LOCUS       DAOQNJ010000577         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_61223_length_1755_cov_8.466471, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000577 DAOQNJ010000000
VERSION     DAOQNJ010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61223_length_1755_cov_8.466471"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1755)
                     /locus_tag="PLG52_13105"
     CDS             complement(<1..>1755)
                     /locus_tag="PLG52_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB42437.1"
                     /translation="VVIPLKIEDHILGALDVQSNQPSAFHPIDLLILRALADNVARAV
                     DGAKLYSALSRRANQLTLVSEVSKSVTSTLDLSKLMRDAASLIHEKFGYPHVSLFTVH
                     PNRRMIMYEAGSGKRSKKLEGFTIPLDDPKGIMPWVARHGKTALANDVSKDSRYIQSP
                     LPPKNTKSELCVPLIFNERVVGLLDIQSDQLNAFTEDDRVMFEAVADTMAAAIRNADL
                     YRSEQWRRQVADSLRDVAGLVSSNAGLDEVLEMILAELDRNLPIDVSAIWLLQEGELC
                     LSAVHGIDGAQLENTCLAFPESTYVMAEALESDVPIIRRPEDPMGPAGLTMNFDPTYS
                     SIVAPLRVGDRPLGVLALAHHTSGRYGHEARAIAGTFANYAAVAMENTRLFDAAQEQA
                     YASAALLQVAQAVVSLSDLDEILGSIIRIMPILVGVKRVVLYRLDMEYQTLQASHEYG
                     FSEEEEAVITEKILPVADFPLIVWALEQNILVTYPLKAEAAPLDWFAIQPGSEEEAHS
                     IDADRLLIAVPLTIKHDIFGVLLIEEDENARRFRSRRLEIINGIAQQAALAIQNDLLQ
                     QEMVVRERLETEVQLARQ"
BASE COUNT          476 a          454 c          412 g          413 t
ORIGIN      
        1 tttggcgagc cagttgaact tccgtttcga ggcgttcgcg caccaccatc tcttgttgaa
       61 gcaaatcgtt ctgaatcgcc agcgctgctt gttgagcaat gccattgata atttccaaac
      121 ggcgtgagcg gaaccggcgc gcattctcat cctcttcgat caagaggacg ccgaaaatat
      181 catgcttgat ggttaacggc accgcgatca acaaacgatc cgcatcgatg ctatgcgctt
      241 cttcctccga cccaggctga atcgcaaacc aatccaacgg cgccgcctca gccttgagtg
      301 gataggtcac taatatattt tgctccagcg cccacacaat taatggaaaa tcagcaacgg
      361 gcaaaatttt ctcggtgata acggcttcct cttcttcaga gaaaccgtac tcgtgcgaag
      421 cttgtaaagt ttgatattcc atatcaaggc ggtacaacac aactcgtttg acacccacca
      481 agatcggcat aatacgaata atggagccga gaatttcatc caaatcgctc aaactgacaa
      541 cagcttgtgc cacttgcaac aaagcagcag aggcataagc ctgctcttga gcagcatcga
      601 agaggcgggt gttttccata gcaactgccg cataatttgc gaaagtaccc gcaatcgcac
      661 gcgcttcatg tccataacgc ccagatgtgt ggtgagccaa tgccaagacg cccaatggac
      721 ggtcacccac ccgcaatggc gccacaatgg atgaatacgt tgggtcaaag ttcattgtca
      781 gcccggcagg acccattggg tcttctgggc ggcggatgat aggaacgtcc gactcaagcg
      841 cctcagccat cacatacgta gattcaggaa atgccagaca ggtattttca agttgtgcac
      901 catcaatgcc atgcacagcc gagagacaca actcaccctc ttgaagcaac caaattgcag
      961 aaacatcaat tggaagattg cggtctagct cagcaaggat catttcaagc acttcatcca
     1021 acccagcatt gctggaaacc aagccagcga catctcgcaa actatcggcg acttggcgac
     1081 gccattgctc agaacgatat aagtctgcat tacggatggc ggcagccatg gtatctgcta
     1141 ccgcttcgaa catgacccgg tcatcttctg tgaatgcatt gagttggtct gactgaatgt
     1201 ccaacagacc aacaacacgc tcgttaaaga tcaacggaac acataactct gacttcgtat
     1261 ttttgggagg caaaggcgat tggatgtaac gcgagtcttt tgaaacgtca ttcgcaagcg
     1321 cggtttttcc atggcgtgcc acccaaggca taatgccttt cgggtcatct aatgggatgg
     1381 tgaagccttc aagttttttg ctacgttttc cacttcccgc ttcgtacata atcatgcgtc
     1441 gattcgggtg gacggtgaaa agcgaaacgt gaggataacc aaatttttcg tgaatcaagc
     1501 tagcagcatc gcgcattagc tttgaaagat caagcgtaga tgtaacactt ttacttactt
     1561 cagaaaccaa ggtcagttga ttggcgcgtc ggctcagggc gctatagagt ttggctccat
     1621 ctacagcgcg ggcaacatta tcggcaaggg ctcgtagaat gagcaagtca ataggatgaa
     1681 aagcgcttgg ctgattgctc tgaacatcca atgcgcccag aatatggtct tctatcttga
     1741 gcggaatgac aactt
//
LOCUS       DAOQNJ010000578         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_61340_length_1753_cov_5.810954, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000578 DAOQNJ010000000
VERSION     DAOQNJ010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61340_length_1753_cov_5.810954"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLG52_13110"
     CDS             complement(<1..707)
                     /locus_tag="PLG52_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42438.1"
                     /translation="MNIDAIKELPALRLENVLRRLKIPWMVFAILIAVLVFIASTKPS
                     LELGVDKLVEYSVHDGVITVYILLMWPISKALLTKSLNSLAALVEEDPTVTAPPSSAN
                     EWRIPVLFGLLMAALDISDTENFLSLSPWLFFISSFMVYAVIAWVVYAVFISARLVTH
                     FVSTISKVRIFDVSPYRPVANWCLSISAMIMLGLSISVLMLGGDFLVIQNLLVYILAA
                     ILGVAVFFAGMWSTHAL"
     gene            complement(863..1273)
                     /locus_tag="PLG52_13115"
     CDS             complement(863..1273)
                     /locus_tag="PLG52_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42439.1"
                     /translation="MKNKKPILLAILLLAALACKTLTQDLPATPTSDYVFPEVTTPLV
                     IEPGSLPKGELGADYEVQILVRDNVTPISSVIISEGALPAGLELVFVDNDTSFTIRGK
                     PEETGSFTFTVFVSCFGTMVSGQTAQVDYTIIVE"
     gene            complement(1263..>1753)
                     /locus_tag="PLG52_13120"
     CDS             complement(1263..>1753)
                     /locus_tag="PLG52_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42440.1"
                     /translation="NVLPWVEVNEMMDDEYRQLVVTEALGHYPKASPELNKFALDVFK
                     KTIQVNGFRSFQSIPLPIAKIQVVKEFKVNELLAEAVICLWVEKQHALVESLAQAARE
                     ANIPVPETWSWQQARYGFVQGEDTPEFDQLATDLSAQKSKPESDHYLLAALWLSNSLH
                     EK"
BASE COUNT          481 a          452 c          429 g          391 t
ORIGIN      
        1 atgagcgcat gcgtgctcca catgccagcg aagaacaccg ccacccccag gatggcagcc
       61 aaaatataaa ccagtaggtt ttgtatcacc agaaaatcgc cgcccaacat cagaacggag
      121 attgaaagcc cgagcatgat catggctgaa atgctcaagc accaatttgc cacagggcgg
      181 tacggtgaga cgtcgaagat gcgtaccttt gagatcgttg aaacaaaatg tgtaaccagc
      241 cgggcgctga taaacacggc atataccacc caggcaatga ccgcatatac catgaaacta
      301 ctgataaaga aaagccaggg agataggctg agaaaattct cggtatcgct gatatccagc
      361 gccgccatga gcagaccgaa caacacaggg atgcgccact catttgcaga agaaggaggc
      421 gccgtgacgg tagggtcttc ttcgaccaga gccgccaagg aattaaggct tttggtcagc
      481 aacgcctttg aaatgggcca catcaaaagg atatagaccg taatgacgcc gtcatggacg
      541 gaatattcaa ccaatttatc aacgcctaat tcaagagaag gtttggtaga agcgataaac
      601 acaagcaccg caatcaaaat cgcaaagacc atccatggga tcttaagacg gcgcaacaca
      661 ttttcaagcc gtaaagcggg caattcttta attgcatcta tgttcataac aaagcctctc
      721 tacctgtttt gtgccaagta taagaaataa tagaattaaa tgaccctaca gctttgtttg
      781 gcatctcaat ttgatagaac cccttcagca ctccgtttaa aaacagacag tcactgcgag
      841 gtgactgtct ggtgatttcc acttattcca caattatcgt atagtccacc tgtgcggttt
      901 gcccgctgac catggtgccg aagcaggaga caaagacggt gaaggtgaag ctaccggttt
      961 cttctggctt gccacggatc gtgaatgagg tatcgttatc cacaaagacc aactccaacc
     1021 cggcgggaag cgcgccctct gaaatgatga cgctgctgat cggggtgacg ttgtcacgga
     1081 cgagaatctg cacctcgtag tcagcaccca gctccccctt tggcaaagag ccgggttcga
     1141 tcacaagcgg agtggtcacc tctgggaaaa cataatcgga cgtgggtgtg gctggcaggt
     1201 cttgcgtcaa cgttttgcaa gcaagggctg ccagcaagag gatagcaagc aggatgggct
     1261 ttttattttt catgaaggct attgcttaac cacaaggcag ccaatagata atggtcggat
     1321 tcgggcttgc tcttctgcgc ggacaaatcc gttgccaatt gatcgaactc gggcgtatct
     1381 tcgccctgca caaagccata gcgggcttgc tgccaactcc atgtttcagg gacagggata
     1441 ttcgcctcgc gtgccgcctg tgccagcgac tcgaccaggg cgtgctgttt ttccacccac
     1501 aaacagatca ccgcttcggc aagcaattcg ttgaccttga actctttcac cacctgtatc
     1561 tttgcgatgg gcagcggaat gctttgaaag gaccgaaagc cattgacctg aatggtcttc
     1621 ttgaaaacgt ccaaggcgaa tttatttaat tcaggcgacg ccttggggta gtgaccgagc
     1681 gcctccgtca ccaccagttg acgatactca tcgtccatca tttcgttcac ttccacccac
     1741 ggcaatacat ttg
//
LOCUS       DAOQNJ010000579         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_61655_length_1748_cov_10.012995, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000579 DAOQNJ010000000
VERSION     DAOQNJ010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61655_length_1748_cov_10.012995"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLG52_13125"
     CDS             <1..270
                     /locus_tag="PLG52_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42441.1"
                     /translation="ITLQAFFFVAMRYMLDVIPTLSLLVIIGFWHGFELFKNKKVYII
                     ITTLLLIHTIACSLLISFSGNLETFKILNPELVREMTWTFNNLLK"
     gene            372..1400
                     /locus_tag="PLG52_13130"
     CDS             372..1400
                     /locus_tag="PLG52_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNB42442.1"
                     /translation="MSRFLDFLNTADLNTLTQVSGVNRQLAGNLIAARPFDAEEDALN
                     VKGMGKATLARLQSFAEAQGNGSESSAMVPVEEEAMPAVMEKNPPAQEDAPKKESFFV
                     RLGRAIRSFFVALLKLIMLAILFVSIGAMFYYGLPYIQRTFIAPVEQNTAQIKTMEAE
                     IETLQTQLDEMNSRVSALEGSVEAHTASIEKLEEMQTALETQLEENQNAVLLELKQEI
                     MFTRALDVLARARLYLAQSNFGLAKTDVQTARDLLAELNAEKEDEVLTKAIERIDLAL
                     DNLPDFPVVASGDLEIAWQILVSGNAPASTATATPTLVPTEPATATPTAVDTAATPTL
                     IPSATATP"
     gene            1499..>1748
                     /locus_tag="PLG52_13135"
     CDS             1499..>1748
                     /locus_tag="PLG52_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNB42443.1"
                     /translation="MAYKIKFGTDGWRGVIAEDYTFDNLRRCAQGFASYMLSQGKKGQ
                     WVVVGHDKRFHSENFAAAVAEVLAGNGLKVYLTDGATPT"
BASE COUNT          453 a          437 c          418 g          440 t
ORIGIN      
        1 atcaccttac aagccttctt cttcgtcgcc atgcgctaca tgttggatgt cattccgaca
       61 ctatcccttc tcgttatcat tggcttttgg catggctttg aacttttcaa gaacaagaaa
      121 gtgtatatca tcatcactac gcttttattg attcatacca tcgcatgtag tttgttgatt
      181 tcgttctcag gcaatttgga aaccttcaag attctcaacc cagaactcgt ccgcgagatg
      241 acgtggactt tcaacaacct tctcaaataa cagatatcca cgcctcgtgc gtgacgtggg
      301 cttgcatcta ttacctcaag ctgattcgca tctgaaacct tttgttgtac aatgaccgta
      361 taggagattc catgagccgc tttctcgatt ttctcaacac tgcagacctg aataccctca
      421 ctcaagtatc tggcgttaac cgtcaactgg cagggaatct cattgccgcg cgtcctttcg
      481 atgcagaaga ggatgcgctc aacgttaaag gcatgggcaa agcaaccctt gcgcgcttgc
      541 agtcttttgc tgaggcgcag ggaaatggtt cagaaagcag tgccatggtt ccggtcgagg
      601 aagaggcgat gcccgccgta atggagaaga atccgcctgc acaggaagat gccccgaaga
      661 aagaatcatt cttcgtgcgt ttagggcggg caattcgatc cttctttgta gcattgttaa
      721 agctgatcat gttggcgatc ttgttcgtat cgattggcgc aatgttctat tatggcttgc
      781 catacattca gcggaccttc attgcgcctg tagaacaaaa cacggcacaa attaaaacca
      841 tggaagcaga gatcgaaacg ctccaaactc agctcgatga aatgaactca cgcgtttctg
      901 cacttgaggg ctcggtagaa gctcatacgg cttcgattga aaaactggaa gagatgcaaa
      961 ccgcgctgga aactcaactc gaagaaaatc agaatgcggt tctgttggaa ctgaaacaag
     1021 agatcatgtt cacccgtgcg ttggatgttc tagcacgtgc acggctgtac ttggcacaaa
     1081 gcaattttgg tttggcaaag accgatgtgc aaaccgcacg agatttgctg gctgaactca
     1141 atgccgaaaa agaagatgag gttctaacca aagccatcga gcggattgac cttgcgcttg
     1201 acaacctgcc agacttccct gtcgttgcct caggagattt ggaaattgcc tggcagattt
     1261 tggtctctgg gaatgcaccc gcatccactg caacggcgac acccacgcta gtccccacag
     1321 agccagcaac tgccactccc acagcagtag atacggcagc tacgcctaca ctaattccat
     1381 ctgcaacggc aaccccgtaa cgcaaaactc cgaggcaatg cctcggagtt tttttatgat
     1441 atttaggctc gtggcgggta taatttcgaa agttatcatt cccaagagga gaattctcat
     1501 ggcatacaaa atcaaattcg gcacagacgg ttggcgcgga gtcattgctg aagattacac
     1561 gttcgacaac ctgcgtcgct gtgcgcaggg gtttgcgtcg tatatgcttt cacaaggtaa
     1621 aaagggacag tgggtcgtcg ttgggcatga caagcgtttc cactccgaaa attttgctgc
     1681 agctgtggca gaagtgttgg ctggcaacgg cttgaaggtc tatctaaccg atggcgcgac
     1741 cccaaccc
//
LOCUS       DAOQNJ010000580         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_61712_length_1747_cov_10.726950, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000580 DAOQNJ010000000
VERSION     DAOQNJ010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61712_length_1747_cov_10.726950"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..980
                     /gene="rnc"
                     /locus_tag="PLG52_13140"
     CDS             279..980
                     /gene="rnc"
                     /locus_tag="PLG52_13140"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNB42444.1"
                     /translation="MTQQSNKRDVESASSLNERLGLSFSNFSLLTTALTHRSYANEHQ
                     DGSLDNERLEFLGDAVIDLIVGEWAYRHFPELPEGDLTKIRAQLVKNENLAKFSQQID
                     LGGALRLGRGEKTTGGSHRESVLGSAFEALLGAIYLDAGFEKVRSFILPFIDPYEPTI
                     LEVLNDPKSTFQEKVQAYKMGPIVYHVIRTSGPDHAKTYDVEVEVNGNIMGQGTGTSK
                     ANAEREAARDALKKL"
     gene            1069..>1747
                     /locus_tag="PLG52_13145"
     CDS             1069..>1747
                     /locus_tag="PLG52_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB42445.1"
                     /translation="MPLRLKSLELQGYKTFASRTTFEFAAGVTAIVGPNGSGKSNIAD
                     SLRWVLGEQSYALLRGKKTEDMIFSGSEHRARAGMAQATINFDNTDQWLPLDFTEVSM
                     GRVAHRDGHNEYIINNQQVRLREMNELLAQAGLSERTYTILGQGLVDASLALKADDRR
                     RLFEEAAGVGLYRARRDDALKRLNDTEHNLERVLDIMSELEPRIRSLERQAKRAIEFA
                     RAQADLKV"
BASE COUNT          450 a          478 c          427 g          392 t
ORIGIN      
        1 cctttatctg attcccccgg ttggtatccc caaacgacac gatctcccca ttgccacgca
       61 cccaaaccca tctccaccgc atgcaaaaat ctggtttcat tgctgacttc gttcataagg
      121 gtctccttga atggcgcaga ttttatctta aaaaaccgct ttgccaaaag attcaggtta
      181 caattcggct cgctaaaatc tatataagaa ctcaaggaac gacatctcct cacttaggag
      241 gtaagtggat atcgctcccg gagttctggg aggttcccgt gacacaacaa tccaataagc
      301 gggatgtgga atccgcttcc agcctgaacg agaggctggg tctgtccttt tccaactttt
      361 ccttgctgac aacggcgcta acccaccgtt cgtacgccaa tgaacatcaa gatggctcat
      421 tggataatga gcgcttggaa tttctcggcg acgccgtcat tgacctcatt gtgggggaat
      481 gggcatatcg tcatttccct gagctgcccg aaggtgacct gaccaagatc cgtgcgcaac
      541 tggtgaagaa tgaaaacctc gccaagtttt cgcaacagat cgaccttggt ggtgctctac
      601 ggttgggacg tggcgaaaag accacgggcg gaagtcaccg tgagagcgtg ctgggttctg
      661 ctttcgaagc cctgcttggt gctatttatt tagatgctgg ttttgaaaag gtgcggagtt
      721 ttattttgcc tttcattgac ccgtatgagc ccaccattct tgaagtgctg aatgacccca
      781 aaagcacttt tcaggaaaag gtgcaagcct acaaaatggg accgatcgtc tatcacgtca
      841 tccgcactag cggacccgac cacgctaaaa cctatgatgt ggaagtggaa gtgaacggca
      901 atattatggg gcagggcaca ggcaccagca aagccaacgc cgagcgcgaa gccgcccgag
      961 atgcattgaa aaaactataa atgccgagtg aaacgtgagc gaatcaaacc tcaccttcac
     1021 tagtaaccaa aatcgtaaat ctgaaatcgt aaatcgaaaa atcgccacat gcctcttcgt
     1081 ctaaaatcac tcgaactgca aggatacaaa accttcgcct cgcgcacaac ctttgagttt
     1141 gctgcaggcg tgactgccat tgtcggaccg aacggttcgg gcaaatcgaa catcgcggac
     1201 tcgctgcgct gggtgctggg cgaacaatct tatgcactct tacgcggcaa aaagaccgaa
     1261 gacatgatct tctccggttc tgaacaccgc gcccgcgcgg gtatggcgca agccaccatc
     1321 aactttgaca acaccgacca atggctgccg ctcgacttca ctgaagtttc gatgggacgc
     1381 gtcgcgcatc gtgacggtca caacgaatac atcatcaata atcaacaagt ccgcttgcgc
     1441 gagatgaacg aattgctcgc ccaagccggt ttgagcgaac ggacgtatac cattcttggt
     1501 caaggtttgg tggatgcctc acttgccctc aaagccgatg accgccgtcg tctatttgaa
     1561 gaagcggctg gtgtcggtct ctaccgcgct cgccgtgacg atgcgctcaa acgcctcaac
     1621 gacaccgaac acaacctcga acgcgtgctc gatattatgt ctgagcttga gccgcgcatc
     1681 cgcagtttgg aacggcaggc aaaacgcgcc atcgaatttg cccgcgccca agcggatttg
     1741 aaagtga
//
LOCUS       DAOQNJ010000581         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_61827_length_1745_cov_7.578698, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000581 DAOQNJ010000000
VERSION     DAOQNJ010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61827_length_1745_cov_7.578698"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PLG52_13150"
     CDS             complement(<1..1051)
                     /locus_tag="PLG52_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42446.1"
                     /translation="MQFRLSPIARQTLLIAVITIIAYGLLLPLTGFYWDDWPFAWIAK
                     FLGPAEFNPAFMPFRPFLGPIFYFTTTVIPLNPLAWQIFALVIRFLIGVTAWWTFTQI
                     FPQRKTLAFFAALLMLVFPGYSQHWVALTHINQELIPFLFYLLSFGYTFKALHTKKKT
                     DTVIALLLQLCGIFPTEYFFGVEGIRFFLLFFVFQGAILERFTKTLKVWFPYLLIWFL
                     NAAWLFYYYKFGPYESYEVAAAQSPNLFYFLTQALDALWKAGFYIWAQVLVLTISTLR
                     APASLLTLGLVAISFILLARILLKSTQAEATEKSFAISLLIIGFFGILLGRLPSLAAN
                     LPLTLQSSYDRFMISI"
     gene            complement(1129..>1745)
                     /locus_tag="PLG52_13155"
     CDS             complement(1129..>1745)
                     /locus_tag="PLG52_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB42447.1"
                     /translation="IVLLNIYGISSNSKWNKYTEIKKGSPMPTTSLTHRLAKREDLIA
                     LKALMDAAISENQKAFLDESQIASSRTIMGLDTQLIDDGTYFIVEIDGQLAGCGGWSR
                     RATMYGSDQTPGRSAALLDPAKDPARIRAMYTHPNHVRKGIGKLIITLCEEAAKAEGF
                     TKMELVATLSGEPLYRTCGFEAYENIVDDRGGAGVPLLRMRKSL"
BASE COUNT          471 a          332 c          540 g          402 t
ORIGIN      
        1 tgatggagat catgaaacgg tcgtaggagg attgcagagt taacggaaga ttggctgcga
       61 gtgaggggag acgtccgagc aggatgccga agaagccgat tatcagtagg gagatggcaa
      121 aggatttttc tgtcgcttcg gcttgggtgg atttgagcag gatgcgggcg aggagaatga
      181 atgagatggc aaccaagccc aaggttaaga gagaggcggg cgcgcggagg gtggaaatag
      241 ttaggacgag gacttgagcc caaatgtaga acccggcttt ccagagagca tccagcgctt
      301 gtgttagaaa gtaaaacaga ttaggcgatt gcgcggcggc aacttcgtag gattcatagg
      361 gaccgaattt atagtaatag aaaagccagg cggcattgag aaaccagata agtaaatagg
      421 ggaaccagac tttgagtgtt ttcgtgaagc gttcgaggat ggcgccttga aaaacgaaga
      481 agagcaaaaa gaagcgaata ccttcgacgc cgaagaaata ttcggtgggg aagatgccac
      541 agagctgtag aagtagtgcg atgaccgtat ctgtcttttt cttggtatgc agggctttga
      601 aggtgtagcc gaaagaaagt agataaaaca gaaaggggat gagctcttgg ttgatatgtg
      661 tgagtgccac ccaatgttgg ctataaccgg ggaaaaccag catgagcaaa gccgcgaaga
      721 atgcaagcgt ttttcgttgt gggaagattt gagtgaaggt ccaccaggcg gtgacaccga
      781 tgagaaagcg gatgaccaac gcaaagattt gccatgccaa cgggttaagc ggaatgaccg
      841 tggtggtgaa ataaaagata ggacctaaaa acgggcgaaa gggcatgaac gccgggttga
      901 actcggcagg accaagaaat ttggcgatcc atgcgaaggg ccagtcgtcc cagtagaagc
      961 cagtgagcgg taagagcaac ccataggcga tgatggtgat aaccgcaatg agcaaggttt
     1021 gacgtgctat gggggagagg cgaaattgca tcctttaatt ataagggatg gcgaggttga
     1081 gtcttaattc ttgccctgta agatgtagag ataaggaata caaaattttt acagcgattt
     1141 tcgcattcgc aagagcggaa cgcccgcacc accgcgatcg tcgacgatat tttcgtaagc
     1201 ttcgaatccg caggtgcgat acagtggttc gccagataag gtggcgacca attccatttt
     1261 tgtgaagcct tctgctttgg cggcttcttc acatagggtg atgatgagtt tcccgattcc
     1321 tttgcggacg tggttaggat gcgtgtacat ggcgcggata cgagccgggt ctttggcggg
     1381 gtcgagcagg gcggcactgc gccctggggt ttggtcactg ccatacatag tggcacgtcg
     1441 actccacccg ccgcagcctg ccagttgacc atcgatttcg acgatgaagt aggtgccgtc
     1501 atcgatgagt tgcgtatcca accccataat ggtacggctc gaagcgatct gactttcatc
     1561 gagaaaggct ttttgatttt ctgagatagc ggcgtccatt aaggctttaa gggctatcag
     1621 gtcttctcgt ttggcaagtc tgtgggttag agaggttgtg ggcatggggc ttcctttttt
     1681 aatttcagtg tatttattcc attttgaatt cgaggagata ccatagatgt tgagcaggac
     1741 gataa
//
LOCUS       DAOQNJ010000582         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_62020_length_1742_cov_8.818020, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000582 DAOQNJ010000000
VERSION     DAOQNJ010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62020_length_1742_cov_8.818020"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLG52_13160"
     CDS             complement(<1..413)
                     /locus_tag="PLG52_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance efflux transporter family
                     protein"
                     /protein_id="HNB42448.1"
                     /translation="MLRLISLGSLAALFFSSTFVINRALSLQGGHWAWTSSLRFGFML
                     IFLVIILLIRDGKKALSDVKKVFSKHWLFWVISGSIGFGVFYSLITFSSQYAAGWVVA
                     TTWQTTILATPIVIALFGRKVPRSGLVFTAIIFIG"
     gene            complement(462..1499)
                     /locus_tag="PLG52_13165"
     CDS             complement(462..1499)
                     /locus_tag="PLG52_13165"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNB42449.1"
                     /translation="MSEELLYYTDYLELDKVLNAQHPKSNNDTDETLFIIIHQAYELW
                     FKMVIHELDHVRRIFDTDNVLDNAGHMSIATHKLKRIVKIFEVVNRQVDILETMTPMD
                     FLEFRDLLVPSSGFQSLQFRIIEAKLGLKMEQRHKANYYKNTRPGGFNQRDYDLISAV
                     ESEATLKGLVIKWAERTPFFDEALWHDFQSKYPLEEGEQRFWSDYKQIYKNSLSGSVE
                     LRQFDELERIFYKEGLGEFSVGAMRAVLFIMMYRNLPIFQQPFDLLSTLIDIDEMLSQ
                     FRYKHMLMARRMIGMRSGTGGTSGAGYLEGALRQHYIFKELTEVSTFLVERSHLPGLP
                     NAVKEKMGFNS"
BASE COUNT          451 a          417 c          447 g          427 t
ORIGIN      
        1 atgccgatga agatgatggc ggtgaagacc aatccgctgc gtggaacttt gcgaccaaag
       61 agtgcaatga cgatgggtgt agcgaggatg gtggtctgcc atgtggtggc aacgacccaa
      121 ccggcggcat attgcgagct gaatgtgatg agtgaataaa aaacgccgaa gccgatgctg
      181 cctgaaatga cccagaacaa ccaatgtttg gaaaagactt ttttcacgtc gctcaatgct
      241 tttttgccat ctcgtatgag caagatgatg accaaaaaga tgagcatgaa accgaaacgc
      301 aaagacgatg tccacgccca gtgaccgcct tgtaaactca aggcgcggtt gatgacgaag
      361 gtgctgctga aaaataaagc ggcaagactg ccgagggaga tgagtcgaag catagtttat
      421 gtatgctctg ttcggggctt agccccgaac agagcatatc cttaagagtt gaaacccatc
      481 ttttctttca ccgcattggg taaaccgggc aaatggctgc gctccactaa aaaggtcgaa
      541 acctcggtaa gttctttgaa aatataatgt tgccgtagtg cgccttcaag atagcccgct
      601 ccgctggtgc cgcctgtacc agagcgcatg ccgatcatac gacgcgccat caacatatgc
      661 ttgtagcgga actgcgaaag catctcgtca atatcgatca gtgtggaaag caaatcgaat
      721 ggctgctgaa aaataggcag gttgcgatac atcatgatga acaagaccgc gcgcatcgcg
      781 ccgactgaaa actcgcccaa gccttcctta taaaaaatgc gctccaactc atcgaactga
      841 cgcagctcca ccgagccgga aagactgttc ttgtaaattt gtttgtagtc gctccagaac
      901 ctttgctcac cttcttccag cgggtatttg gattggaagt catgccacag ggcttcatcg
      961 aagaaggggg ttcgctctgc ccacttgatg accagccctt tgagcgtcgc ctcagactcg
     1021 acggcggaga ttaaatcgta atcccgttgg ttgaatccac ccggacgcgt gttcttgtag
     1081 tagttggctt tgtgacgctg ttccatcttg agccccaact tggcttcgat aatgcggaat
     1141 tgcaggcttt ggaatcccga agatggcaca agcaggtcgc gaaactcaag gaagtccatg
     1201 ggcgtcatcg tttcgagaat atcaacctga cggttgacca cctcaaatat ctttacgatg
     1261 cgtttcaact tgtgggtggc gatgctcatg tgtccggcgt tgtccaaaac attatctgta
     1321 tcaaaaatcc ggcggacatg gtcaagttca tgaatgacca ttttgaacca cagctcatat
     1381 gcctggtgaa tgatgatgaa aagagtttca tctgtgtcat tattgctctt tgggtgctgc
     1441 gcgttgagca ctttgtccag ctcaagatag tcggtgtaat acaaaagttc ttcgctcatg
     1501 ggttcctcgc tttggcaggg aagttggttt gtgcctggct gattctatct ttaaatacga
     1561 tggtggacga aagaccagcc acggtctacc gtccaccgtc catcgtctac ggtctgatta
     1621 cagcggctca aaccgcgccg tgaaatgacg caacaactcg ggggcgtagg tgaacttgta
     1681 gccttttact ttatcggcgc gtgacttgat agcggcaaaa caatctgcta cataatcgag
     1741 at
//
LOCUS       DAOQNJ010000583         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_62193_length_1739_cov_10.940618, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000583 DAOQNJ010000000
VERSION     DAOQNJ010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62193_length_1739_cov_10.940618"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1358
                     /locus_tag="PLG52_13170"
     CDS             174..1358
                     /locus_tag="PLG52_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB42450.1"
                     /translation="MSKNQKPSVDPVIIVMLGVIFLAVAIAMIVFQPTSNSINLTADA
                     SVMSQLVVAFVTGITTGGLSCLAVQGGLLASSLAYQIEQDYSAQAKNKKATRTNSAMP
                     IALFLIAKLVAYTLMGALLGWLGSFLTLSPLTRAILMIAIGIFMVGNALRMFNVHPIF
                     RYFSIEPPKFITRYIRRTAKGTDTATPIFLGLLTIFIPCGVTQAMMATALGTGSVLMG
                     AALMFAFTLGTSPVFFIVAYLATELGAKLEKFFMRFVAVVVLILGLVTLDGGLNLMGS
                     PLSLQNMTRGWFAPQAESNAITESVQPSAASDEIILSVENNGYFPAILKAPAGKDLTL
                     SLVTNKTYSCARDFVIPDLNFYELLPDTGTVQVNIPAQKAGSTLFFTCSMGMYTGQIV
                     FE"
     gene            1392..1604
                     /locus_tag="PLG52_13175"
     CDS             1392..1604
                     /locus_tag="PLG52_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNB42451.1"
                     /translation="MIKKTFKIPDMSCTNCAMKIESLEDDLDGVKEVNASYHKLEMVI
                     EYDEAKLTDEQIVTAVKKKGYTAVAA"
BASE COUNT          430 a          453 c          392 g          464 t
ORIGIN      
        1 gccgatgtat tggctgccga aactgccggt gaattgatca tcgaacaacc cggtgtagtc
       61 atcaatatgc ccttcgtcgt ttgggatggc ggtcagcgat aataaactta tgtacggctt
      121 acgcattaca aatttgcgta agccgtaatg tgtaaaaact ttattgaggt cgaatgagta
      181 aaaatcaaaa gccttctgta gacccggtca ttattgtgat gcttggcgta attttccttg
      241 ccgtggctat tgccatgatc gtgtttcagc ctactagcaa ttccatcaac ctcactgccg
      301 atgccagtgt gatgagccaa cttgtagtgg cattcgttac cggcattact acgggcgggt
      361 tgagctgcct tgcagtgcaa ggcggtttgc ttgccagttc acttgcctat cagatcgagc
      421 aggattacag cgcacaagcc aaaaataaaa aagccacgcg tacaaattct gccatgccga
      481 tcgcactctt tcttattgcc aaactcgttg catatacttt gatgggggcg ttgctcggct
      541 ggctgggttc tttcctcacc ttaagtccgc tcacgcgcgc catcctgatg atcgccatcg
      601 gcattttcat ggtgggtaac gcgttgcgca tgttcaacgt ccacccgatc tttcgttact
      661 tttccattga gccgccgaag ttcatcacac gatatatccg ccgaactgcc aaaggcacag
      721 ataccgccac acccatcttc ctcggcttgc tcacgatctt cattccttgt ggcgtcactc
      781 aagcgatgat ggcaaccgct ctcggcacag ggagtgtact tatgggtgca gcattgatgt
      841 tcgccttcac ccttggcacc agcccggtat ttttcatcgt tgcatatctc gccacagagc
      901 tcggcgcaaa acttgaaaaa ttcttcatgc gatttgtcgc cgtggtggtt ttgatcttgg
      961 gactcgtcac ccttgatggt ggactcaact taatgggctc gcccctctcg cttcaaaaca
     1021 tgactcgcgg ctggttcgcc ccacaagcag aatccaacgc tataacagaa tccgtccagc
     1081 cctctgccgc aagtgatgaa attatcctaa gcgtagagaa caacgggtat ttccctgcca
     1141 ttctcaaagc tccagcaggc aaagacctga ccttaagcct cgtcacgaat aaaacctatt
     1201 cctgtgcccg cgactttgtt attcccgatt taaatttcta cgaattgctt cccgatacag
     1261 gtactgttca agtgaacatc ccagcgcaga aagccggctc aaccttgttc ttcacttgct
     1321 ccatgggtat gtacaccggg cagattgtgt tcgaatgata aacgtagggt cgcccctaca
     1381 ttggagatga aatgattaag aaaaccttca aaatccccga tatgagttgt accaactgcg
     1441 ccatgaagat cgagtctctg gaagatgacc tggatggcgt gaaagaggtc aacgccagct
     1501 atcacaaatt ggagatggtc atcgagtacg atgaggcgaa actaacggat gagcaaatcg
     1561 tgaccgctgt aaaaaagaag ggctataccg cagtcgccgc ataagttgac cttttaaaag
     1621 gcattatcaa ggcatgtgga gcaataaatg tgcgcaaccc atttaagctc cacatgccta
     1681 cttaatttaa agattttgtt tgttttcatt ttgacccctt tccgttatac tcgcgccca
//
LOCUS       DAOQNJ010000584         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_62194_length_1739_cov_6.109857, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000584 DAOQNJ010000000
VERSION     DAOQNJ010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62194_length_1739_cov_6.109857"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLG52_13180"
     CDS             <1..407
                     /locus_tag="PLG52_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42452.1"
                     /translation="PVEHPFIYTSIKPICSKGNLKPDMPFTFMLGSRSEGAGQSRVLH
                     TYIGVFIPDHPRLTDAWLNTWKHKVAERGDNWAQHSDVEPHEKSWGLMGAPEDLPIVA
                     KRINNGVLIAWSGLHIRKVFEARLDELKQTLS"
     gene            545..>1739
                     /locus_tag="PLG52_13185"
     CDS             545..>1739
                     /locus_tag="PLG52_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNB42453.1"
                     /translation="MSNEYDLKSVKLPYLAGGVLKLFASLVEGSLQSALIPSLFESAG
                     LTWLRKQQIDEAPTYLPIHYVGELQTKDAAIPETQWPAMPSQTSGFQFASIFDFAKAY
                     RAGTTTPEEVAKKVLDAIEASDKSDAPLRAFIVVNRDEVMRQARESTERFKNGKPLSI
                     FDGVPVAIKDEIDVTPYPTTVGTSFLGKSPAKEDSEVAARLRKAGAMLVGKTNMHEIG
                     INVFGLNPHHGTTRNPYNTAHFTGGSSSGSATAVAAGLVPVAIGADGGGSIRIPAAFC
                     GLVGLKATFGRVSEYGAFPLDWSVAHIGPIAGTATDVTLTYALLAGPDLKDEVSLHQP
                     LPTLESWDNVNLKGLKIGIYRPWFEHAEKEVVDACTAMLKKFEEMGCEIKEIVIPNLE
                     ANRVAH"
BASE COUNT          473 a          435 c          422 g          409 t
ORIGIN      
        1 cccctgtaga gcatcctttt atttacacca gcataaaacc gatctgttca aagggtaacc
       61 taaagcctga tatgcctttc acattcatgc ttggttccag gtctgaaggt gccgggcaat
      121 ctcgagttct ccacacctac attggggtct tcatcccaga ccacccacgg ctgaccgatg
      181 cctggctcaa tacttggaaa cacaaagtag ccgagcgtgg tgataactgg gcacaacatt
      241 ccgatgtgga accccatgaa aaaagctggg gactgatggg agcccctgaa gatttaccca
      301 ttgttgccaa acgcatcaac aatggcgttc taattgcatg gtcagggctg catattcgaa
      361 aagtatttga agcacggcta gacgaattaa agcaaacgct ctcctaacaa ccaaatcata
      421 tgggatacaa ccccgcttcc gcctcatttg aagcggcttc aacatcccaa actgatactc
      481 acactgacaa gtgatacccg cagtgatttg caccacataa cagcattttc cttgaggaga
      541 aacaatgagc aatgaatacg atctcaaatc tgtgaagctg ccctatctgg cgggcggggt
      601 attaaagttg tttgcatcat tagtggaagg atctttgcaa agcgcgttga ttcccagctt
      661 gtttgaaagc gccggtctga cttggctacg caagcaacaa attgatgaag ctcccactta
      721 tttgccgatt cattatgttg gtgaattaca aaccaaagac gcagcgatcc ctgaaacaca
      781 atggcccgca atgccatctc aaacaagcgg atttcaattc gcaagcatat tcgattttgc
      841 caaagcctat cgtgctggaa caaccacacc tgaagaggtg gcaaagaagg tattagatgc
      901 cattgaagcc agtgacaagt ctgatgcgcc attacgggca ttcatcgttg tgaaccgaga
      961 cgaggttatg cgccaagctc gcgaatcgac ggagcgtttc aagaatggca aaccgctcag
     1021 catcttcgac ggtgtgccgg tggcgatcaa agatgaaatc gatgtaacgc catatccaac
     1081 aacagtcggc acatctttcc tcggaaaatc accagccaag gaagattcag aagtggcggc
     1141 gcgtttacga aaagcgggtg ccatgttggt cggcaagacg aatatgcatg agatcggtat
     1201 caatgtcttt gggttgaacc cacatcacgg cacgacccgc aacccataca acaccgctca
     1261 ctttacggga ggaagttcaa gcggttctgc cactgccgtt gcagcaggat tggtaccagt
     1321 tgccatcggt gccgatggcg gcgggtcgat tcgtattcca gcagcgtttt gcggcttggt
     1381 cggcttgaaa gccacatttg ggcgcgtcag tgaatatggt gcatttccct tggattggag
     1441 cgtggcgcat attggtccca ttgctggcac agccaccgat gtcacattga cctacgccct
     1501 tctggcagga cccgacttga aagatgaggt ttctcttcac cagcctttgc ccaccttaga
     1561 gagctgggat aatgtcaatc tcaaaggact caaaatcggt atctatcgtc cgtggtttga
     1621 gcatgcagaa aaagaagttg ttgatgcctg tactgccatg ctcaaaaagt ttgaagagat
     1681 gggttgtgag atcaaagaga tcgtcatccc gaaccttgaa gcaaaccgag ttgcccata
//
LOCUS       DAOQNJ010000585         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_62246_length_1738_cov_9.428402, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000585 DAOQNJ010000000
VERSION     DAOQNJ010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62246_length_1738_cov_9.428402"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLG52_13190"
     CDS             <1..543
                     /locus_tag="PLG52_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNB42454.1"
                     /translation="CEIKEIVIPNLEANRVAHTVTIISEMVQGMSATYDQHHKEHGLD
                     VRINLALGRKFSADDYVKAQRIRTRMIHHFNNAFKEVDVILTPTTAIAAPEIKKGALP
                     DGDSDLSTTVEIMRFATAGNLTGLPAITFPVGYTQKGLPIGLQAMGRAWDEVTLLRLA
                     LASEKVIERKAPQVHFKILE"
     gene            691..1086
                     /locus_tag="PLG52_13195"
     CDS             691..1086
                     /locus_tag="PLG52_13195"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB42455.1"
                     /translation="MAKKAELKTKKNEASVQDFIETVQDEQVRTDCLEIAKMMEQATK
                     AKGKMWGSSIVGFGEQHLVYESGRELDWMKIGFSPRKQNITLYVHLEEGLLKKLGKFT
                     TGKGCLYIKKLADVDKKVLKELIVASATS"
     gene            1083..1379
                     /locus_tag="PLG52_13200"
     CDS             1083..1379
                     /locus_tag="PLG52_13200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB42456.1"
                     /translation="MSLQKDPEALEKKALHRLVHFQNKHVLEVGCGEGRLTFKYAGSA
                     KQVTAFDPDHDALRIARADCPPALRPIIHFTGASASHIPYAKETFDIAILAWSL"
     gene            1398..>1738
                     /locus_tag="PLG52_13205"
     CDS             1398..>1738
                     /locus_tag="PLG52_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42457.1"
                     /translation="MVHALSEIHRVLKPNGTLLDLRPLEDSWSVEVVSSAGWQVAGRL
                     IDMPQGVADDAAANKAMRDVEARGWFIQKISEEFPYFYSWDTPTEMKAFMENEWEGFE
                     KLDEEVYRTTAS"
BASE COUNT          499 a          384 c          441 g          414 t
ORIGIN      
        1 tgtgagatca aagagatcgt catcccgaac cttgaagcaa accgagttgc ccatacggtg
       61 accatcatca gtgagatggt gcaagggatg agcgcaacct atgaccaaca ccacaaagaa
      121 cacgggttgg atgtacgcat caatcttgcg ctcgggcgaa agttcagcgc agacgattat
      181 gtcaaagcgc agcgtattcg gacacggatg attcatcact ttaataatgc attcaaagaa
      241 gtggatgtca ttctgacccc caccactgcc attgccgctc cagagatcaa aaaaggcgct
      301 ctgcctgatg gcgattctga cttgagcacc actgtggaaa tcatgcgctt cgccaccgcc
      361 gggaatttaa ccggcttacc tgctattacc ttcccggttg gttatacaca aaagggttta
      421 ccgattgggc tacaagctat gggacgagca tgggatgaag ttactctact acggttggct
      481 ttagcttcgg aaaaagttat tgaacgtaaa gctccacagg ttcatttcaa aatattggag
      541 tgagttacag aaacatactc aataagttta aactctctac ggcaaaaggc tttttattcg
      601 ccaattaggc aaacttttcc tttccaaatt tagaacaaat aagctaaaat atatcctatt
      661 gtccagccaa gtaaagaaag gataaaaccc atggcaaaga aagcagaatt aaagaccaaa
      721 aagaatgaag ccagcgttca agactttata gagaccgtcc aagatgagca agtgcgcacc
      781 gattgtttag agatcgcgaa gatgatggag caagccacca aagcaaaagg caaaatgtgg
      841 ggctccagca ttgtaggctt tggcgaacag catttggttt atgaaagcgg gcgtgagttg
      901 gattggatga agatcggctt ttcgccacgg aaacaaaata tcaccttgta tgttcatctt
      961 gaagaaggct tgctaaagaa gctcggcaag ttcaccacgg gcaagggatg tttgtacatc
     1021 aaaaaactgg ctgatgttga taagaaagtt ttgaaagagt taattgttgc ctcagcaacc
     1081 tcatgagttt gcaaaaagac cctgaagcgc ttgaaaagaa agcccttcat cgcttggttc
     1141 actttcagaa taagcatgtt ctggaggtcg gctgcggtga agggcgctta acgtttaagt
     1201 atgccggttc tgctaaacaa gtcacggcct tcgaccccga ccatgacgcc ttgcgcatcg
     1261 cccgggcaga ctgtcccccc gccctgcgtc ccatcataca ttttacgggc gcaagtgcaa
     1321 gccatattcc ttacgcaaaa gagacttttg atattgccat cctcgcttgg tcactctgat
     1381 gactggctca tgagagcatg gttcatgctc tgagtgaaat tcaccgggta cttaagccga
     1441 acgggactct gcttgatcta cgtccattgg aggattcgtg gtcggtggag gtggtttcat
     1501 cggcgggctg gcaggtggcg gggcgtttga tcgatatgcc tcagggggtg gcagatgatg
     1561 cagcggcgaa taaagccatg agagacgtag aggctcgcgg atggttcatc caaaaaataa
     1621 gtgaagagtt cccatacttt tattcttggg acacgcccac agagatgaaa gcgtttatgg
     1681 aaaacgaatg ggaggggttt gagaaattgg acgaagaggt ataccgcacg actgcctc
//
LOCUS       DAOQNJ010000586         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_62403_length_1736_cov_8.579417, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000586 DAOQNJ010000000
VERSION     DAOQNJ010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62403_length_1736_cov_8.579417"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1736
                     /locus_tag="PLG52_13210"
     CDS             <1..>1736
                     /locus_tag="PLG52_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNB42458.1"
                     /translation="QLNWDLVVIDEAHRLRNVYKASNKIANAIKQAINPFPKVLLTAT
                     PLQNSLLELYGLVSIIDEYAFGDFASYKAQFARLGNDVNFEALRERLKPICKRTLRKQ
                     VLEYVKYTNRHAVVQEFVPSEDEQRLYDLVSAYLQKDKLYALPASQRALVTLILRKLL
                     ASSTYAISGTLDSLAKRLETAAMEAETVDVPPADLPENWEDFDELADEWDEDEEGEQK
                     EKVRLTPQQIAELKQEIEELREFHRLAVSIVRNSKGEVLLTALRKGFAEASKAQQNQG
                     AAVLQQKAVIFTESRRTQEYLFNVLENTEFAGKVMMFNGTNTDARSKEIYRNWLGKYQ
                     GTDRVSGSPTADMRAALVECFRDEAAIMIATEAAAEGINLQFCNLVVNYDMPWNPQRI
                     EQRIGRCHRYGQKFDVVVVNFLNKSNAADQRVYELLNEKFRLFDGVFGSSDEILGAVE
                     SGVDFEKRIAEIYQKCRSVEQIQFEFDQLQRELEAEITEGQKDAREKLLNNFDQEVVE
                     KVRIQSNDYLDRFNQQLWLLTRSLLKDYAHFNEHGYSFTLHTNPFPGETIHPGPYRMG
                     KHVEEVNTYRVG"
BASE COUNT          473 a          399 c          464 g          400 t
ORIGIN      
        1 ggcagttgaa ttgggatctg gtcgtgatcg atgaagctca ccgtttgcga aatgtgtaca
       61 aggcgtcgaa caagattgcc aatgccatca agcaggcgat caatccattc ccaaaagtgc
      121 ttttgactgc cactcctctg cagaactctt tattggaact ctatgggctg gtcagtatta
      181 ttgatgaata cgcctttgga gattttgcaa gttacaaggc gcaatttgcc cgcttgggca
      241 acgacgtaaa ttttgaggct ttgcgtgaac ggttaaaacc catttgcaaa cgaaccctgc
      301 gcaaacaggt gcttgagtat gtcaagtaca ccaaccgcca tgccgtagtt caagaatttg
      361 tgccatccga agatgaacaa cgtctttatg acttggtatc tgcctatttg cagaaggata
      421 agttatacgc gctgccggcc agccagcgcg cattggtcac tttgatcctg cgcaaattac
      481 tggcgtcttc cacttacgct atttcaggga cattggatag tctggcaaaa cggctcgaaa
      541 ctgccgcaat ggaagcagag accgtagatg ttccgcctgc tgatctgcct gaaaattggg
      601 aagacttcga cgaactggca gatgaatggg atgaagacga agaaggggag caaaaggaaa
      661 aagtacgatt aactccacag cagatcgccg aactcaagca ggagatcgaa gagttacgag
      721 aatttcaccg attggctgtg tccattgtca ggaactcaaa aggcgaagta ttactgactg
      781 ccctgcgtaa aggctttgct gaggcgagca aggcgcaaca gaatcaaggg gcggctgtgc
      841 tccaacagaa ggctgtgatc ttcacggagt cgcgccgaac acaagagtat ctgttcaatg
      901 tgttagagaa caccgaattt gccggcaagg tgatgatgtt caacggtacg aatactgatg
      961 cccgctccaa agagatttac cgaaattggt tgggaaagta tcagggcacg gatcgtgtca
     1021 gtggctcacc cacagcggac atgcgtgccg cattggtgga atgctttcgc gatgaagcag
     1081 ccatcatgat cgctaccgaa gcggcggcgg aaggtatcaa cctccagttc tgcaacctgg
     1141 tggtgaacta tgatatgccc tggaacccac agcgaatcga acagcggatc ggacgctgtc
     1201 atcgttatgg tcaaaagttc gatgtggtcg tggtgaattt cctgaacaag agcaatgcag
     1261 cggatcagcg cgtgtatgaa ctgctcaacg agaagttccg cctgtttgat ggtgtctttg
     1321 gctccagtga cgaaatcctt ggcgcggtag aatctggtgt ggacttcgag aagcgcatcg
     1381 cggagatcta ccagaaatgc cgcagtgtgg agcaaattca atttgagttt gaccaacttc
     1441 aaagggagtt ggaggcggag atcaccgaag gacaaaagga tgcccgcgag aaattgctca
     1501 acaatttcga tcaggaagtc gtcgagaaag tccgcattca gagcaacgat tatctggatc
     1561 gtttcaatca acaactctgg ctgttgaccc gttcacttct gaaggattat gctcacttca
     1621 acgagcatgg atacagtttc actctccaca caaacccctt ccctggcgaa acgatccacc
     1681 caggtccgta tcgtatggga aaacatgtgg aagaggtaaa tacctatcgg gtcggg
//
LOCUS       DAOQNJ010000587         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_63087_length_1726_cov_7.844405, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000587 DAOQNJ010000000
VERSION     DAOQNJ010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63087_length_1726_cov_7.844405"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLG52_13215"
     CDS             complement(<1..319)
                     /locus_tag="PLG52_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42459.1"
                     /translation="MNKTLKAALLVIGILIVGLGSFAGGFVTGHMMPFGGSAFSLGEP
                     PTAVAPPTTSPEQQSATPEDMQVLFAPFWEAWNLVHENYVDQPVDDVALMRGAINGMM
                     QSLG"
     gene            complement(536..778)
                     /locus_tag="PLG52_13220"
     CDS             complement(536..778)
                     /locus_tag="PLG52_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42460.1"
                     /translation="MENSRLAIAALIFILLILGINFVMYGIVRGVTRGGKNDPLLNIM
                     KAMNSTQQKKNDEMEELRRTVDELNKGKEKTNGESE"
     gene            complement(768..1412)
                     /locus_tag="PLG52_13225"
     CDS             complement(768..1412)
                     /locus_tag="PLG52_13225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HNB42461.1"
                     /translation="MYFTTIFFDLDDTLYPSNTGLWKAIKERMNIYMRERMGFPEDQI
                     AYIREKYFLQYGTTLRGLQAHHEIDVQDFLAFVHDLPLKEYLTPNPTLRSIIASLPTR
                     NLIFTNADSAHADRVLTVLGLRDLFEIIVDVNAIDPYCKPMPESFQIAMQAAGETDPS
                     KCVMIDDIHRTTRAAKEAGLFSILYNEEFPAGSADAQLTDWNQFIPILESHHGK"
     gene            1433..>1726
                     /locus_tag="PLG52_13230"
     CDS             1433..>1726
                     /locus_tag="PLG52_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB42462.1"
                     /translation="MPIRLLSSEVSSQIAAGEVVERPASVIKELTENSLDAGATTISI
                     SIADAGRTLLEVADDGHGIPADELELAAARHATSKLTQSDDLFHIQTLGFRGEA"
BASE COUNT          408 a          410 c          460 g          448 t
ORIGIN      
        1 ctcccagtga ctgcatcatg ccattgatgg cgccgcgcat gagcgccaca tcatcaactg
       61 gctggtcaac ataattctca tgaactagat tccacgcctc ccaaaatggg gcaaagagaa
      121 cttgcatatc ctcaggggtg gcagattgct gctctggtga ggtggttggc ggagcaacgg
      181 cagttggcgg ctcgcccagg ctgaaggctg agcctccaaa aggcatcata tgcccggtta
      241 cgaaaccgcc tgcaaaggag cccagtccaa cgatcaatat tcctattaca agcagtgctg
      301 ctttcaatgt tttattcaca aatgcctcca attttggctt tatatcgcac tcggttggga
      361 attacgcttt ttaggataaa aaggaagcgt aactcttaac cgttgggatt atatctacgc
      421 aggaagatac cagtcggtta ttaaagattg ctgaattaat tgttcgattt ctgtaagaat
      481 cgctgttccc gtactcccat cagatacaag acctgaaatg agtgcaagct ggttactatt
      541 cagattcccc gtttgttttt tccttaccct tgttcaactc atcgaccgtg cggcgtaatt
      601 cttccatttc atcgttcttt ttttgttggg tcgagttcat ggctttcatg atattcagca
      661 agggatcatt cttgccacca cgtgtcaccc cgcgcacaat gccatacatg acaaaattaa
      721 ttcctaagat caatagaata aagatcaaag cggctattgc aagtcggcta ttttccatga
      781 tgactctcca aaatggggat gaactggttc cagtctgtga gttgcgcatc ggcgctgcca
      841 gcggggaact cttcgttata tagaatactg aacaagcccg cttctttcgc ggcgcgggtg
      901 gtgcggtgga tgtcgtcgat catgacgcat ttggaggggt cggtttcccc cgcggcttgc
      961 atggcgatct gaaacgactc gggcatgggc ttgcaatacg ggtcgatggc gttcacgtct
     1021 acgatgatct caaagaggtc acgcagacca agcacggtca acactctgtc ggcgtgggca
     1081 gagtccgcat tggtgaagat caggttgcgg gtcgggagag aagcgatgat cgagcgtaga
     1141 gtcgggttgg gggtgagata ctctttcagc ggcagatcat gcacgaaggc gaggaaatcc
     1201 tgcacgtcaa tttcgtgatg ggcttgaagt ccgcgcagag tggtgccgta ttgcaaaaaa
     1261 tatttttcac ggatatatgc gatctggtct tctggaaaac ccatgcgttc gcgcatgtaa
     1321 atgttcatgc gttctttaat ggctttccac aaaccggtat tggatggata cagcgtgtcg
     1381 tcgaggtcga agaaaatggt ggtgaaatac atccggcgat tataatcgtg ccatgcctat
     1441 tcgattgctt tcttccgaag tctcgtcgca gatcgctgca ggtgaagtgg tggaacgccc
     1501 agcttctgtt atcaaagagt tgaccgaaaa ctcactggat gctggcgcta cgaccatttc
     1561 gatttccatt gccgacgccg ggcgaacgtt gctggaagtg gcggatgatg ggcatggcat
     1621 accggctgat gaattggaac tggcggcggc tcgacacgca acctcgaagt tgactcagtc
     1681 cgatgacctg ttccacatcc agaccttggg cttccgaggc gaggcg
//
LOCUS       DAOQNJ010000588         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_64431_length_1706_cov_9.745609, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000588 DAOQNJ010000000
VERSION     DAOQNJ010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64431_length_1706_cov_9.745609"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PLG52_13235"
     CDS             <1..965
                     /locus_tag="PLG52_13235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNB42463.1"
                     /translation="LALLIKMNCSIVIRAYNEAQHLGRLLEGIKQQTIKDVEIILVDS
                     GSTDSTVSIAETYGAKIVHIPSAEFTFGRSLNFGIKAASRELIVIASAHVYPVYPDWL
                     ETLLHPFQDEQIALTYGKQRGYEDSKYSEHQIFHQWYPDASNLDQVTAFCNNANSAIR
                     KRLWDLHPYDETLTGLEDLEWSKWAKAQGHKIAYIAEAEIVHIHNETPKGVYNRYRRE
                     AMALRRIYPESNFNLYDFLRLTITNILSDLWHAVRERVLLKSFSSIFWFRFAQFRGTY
                     MGHRETSLVTPQLRETFYYARERKKKDERKRDVEPIQYNKGNDE"
     gene            1003..1587
                     /locus_tag="PLG52_13240"
     CDS             1003..1587
                     /locus_tag="PLG52_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNB42464.1"
                     /translation="MATPPPNSVHPKSRAEWRKWLEENQARKEGIWLISYKKDTGKPR
                     FDYDESVEEALCFGWVDSKPAKLDEERSMLWFAPRKPKTNWSKLNKDRVEKMIKAGLM
                     APAGLAKVEAAKKDGSWNALDGVEALEIPPDLAKALSKNKTAKEYFEAFPRSVKRGIL
                     EWILNAKRPETRAKRIEETVTLAEKNIRANQWRQ"
BASE COUNT          502 a          424 c          393 g          387 t
ORIGIN      
        1 tcctcgccct attgataaaa atgaattgct ccatcgtcat ccgcgcctat aacgaagccc
       61 agcatcttgg acgtttgctg gaaggaatta aacagcagac cataaaagat gtagaaatca
      121 tattagttga ttcaggctct actgactcaa ccgtttctat cgcggaaact tatggcgcga
      181 agatcgtcca catcccttca gcggagttta catttgggcg ttcgctcaac ttcgggatta
      241 aagctgcaag ccgcgaattg atcgtcatcg ccagtgcgca tgtctatcct gtctatcccg
      301 attggctaga aacgttactc catccatttc aagatgagca gattgcacta acctacggca
      361 agcagcgtgg gtatgaagac tcaaaatatt cagaacacca gatctttcat caatggtatc
      421 ccgatgcgag caacctcgat caggtcactg cgttttgtaa caatgccaac tccgccatcc
      481 gcaagcgttt atgggatttg catccctacg acgaaacgct gaccggcttg gaagatttgg
      541 aatggagcaa gtgggcaaaa gcgcagggac ataaaattgc ctacattgct gaagccgaga
      601 tcgtccacat ccacaatgag acgcccaaag gggtttacaa ccgctatcgc cgtgaagcca
      661 tggcgttgcg acgtatctat cctgagtcga atttcaattt atatgatttc ctgcgcttga
      721 ccatcacgaa tattctgagt gatctttggc atgccgtccg cgaaagagtt ttgttgaaaa
      781 gtttttcatc catcttctgg tttcgctttg cccaatttcg aggaacatat atgggacacc
      841 gcgagacgag tctagtcacg ccgcagttaa gagagacttt ttattatgct cgggaacgaa
      901 agaagaaaga tgaaagaaaa cgggacgtag aaccgataca atacaacaaa gggaatgatg
      961 aataggtaat agaccgttga aaaagactgt ctattatgta ctatggctac tcctcccccc
     1021 aactccgttc accctaaatc acgagcagaa tggcgcaagt ggctggaaga gaaccaggcg
     1081 cgcaaagaag gtatttggct aatcagctac aagaaagata ccggcaagcc gcgcttcgac
     1141 tatgacgaat ccgtggaaga agccctatgc tttggctggg tagatagcaa acctgccaaa
     1201 cttgacgaag aacgctccat gttgtggttt gccccgcgca agcccaaaac gaattggtcg
     1261 aagctcaata aagaccgcgt ggaaaaaatg atcaaagccg ggctcatggc tcctgccggt
     1321 ctggctaaag tggaagccgc caaaaaagac ggctcctgga acgccctcga tggtgtagaa
     1381 gcacttgaaa ttccgcccga ccttgccaaa gccctctcta aaaataaaac agctaaagaa
     1441 tactttgaag cattcccgcg ctcggtcaaa cgcggcatcc tggaatggat actcaacgcc
     1501 aaacgaccgg agacacgggc aaaacgaatt gaagagacag tgacgctagc agagaaaaat
     1561 atccgcgcaa atcagtggcg gcagtaagtc caaagacata taaggatatt cgatataatt
     1621 tccactatgc ctaccctctc tctcaaacct gcttctgaat acaccatccc cttcctcgcg
     1681 gacttgatga ctcgcggatt tgaagg
//
LOCUS       DAOQNJ010000589         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_64569_length_1704_cov_10.653123, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000589 DAOQNJ010000000
VERSION     DAOQNJ010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64569_length_1704_cov_10.653123"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLG52_13245"
     CDS             complement(<1..234)
                     /locus_tag="PLG52_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42465.1"
                     /translation="MDRFEFLKNIKRPTVSQIIFWVVTIALSITAFIVANNFTQCWTF
                     TKLPGIPPAKCGVTTDSTGFNPETGATQEAVLPD"
     gene            complement(247..810)
                     /locus_tag="PLG52_13250"
     CDS             complement(247..810)
                     /locus_tag="PLG52_13250"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNB42466.1"
                     /translation="MKDLFAELTSGDDERAEQAIPAIVDAGAAAIPALLELTYSENTD
                     TRWWAVRALAASPHTQTSDLISTLSDSSPEVRAAAALALSQHPGEEAVSALAQNLRDS
                     DPLAAGLAANALVKIGKAAVPSLIEAAKDGEQGIRILALRALSELRDHRAIPVMMQCI
                     QEDSAVLGYWAQMGLERLGLDMVYIKP"
     gene            complement(817..1035)
                     /locus_tag="PLG52_13255"
     CDS             complement(817..1035)
                     /locus_tag="PLG52_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42467.1"
                     /translation="MVSTELLEILRCPSCVREKDGLLKLHKDAWLICQDCGRKYPIVD
                     DIPVMLIEEGDKWVNTAQDALPVPPPAK"
     gene            complement(1090..>1704)
                     /locus_tag="PLG52_13260"
     CDS             complement(1090..>1704)
                     /locus_tag="PLG52_13260"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNB42468.1"
                     /translation="EALIIYGGRTIKGVGGGVCQVSTTLFRTVFFAGYPVVERYSHAY
                     RVPYYEMDQSGSVDGNLVGMDATVYFPLVDFKFKNDTPYWMLMETNVNVAARTITWKI
                     YSTSDGRSVTWETTGAQNTVPAPETVFEENVDLKTNELKQVDYAAEGADVTVTRTVWR
                     NGAVYFTDQIQTHYEPWAAVCQYGPGTEDPRRQARKSEVCGFSS"
BASE COUNT          414 a          436 c          397 g          457 t
ORIGIN      
        1 atcaggaaga acagcttctt gagtagcgcc cgtttcaggg ttgaaaccgg tgctatccgt
       61 ggtcacacca cattttgcag gtggaatgcc cggcaacttc gtaaacgtcc aacactgggt
      121 gaagttattc gccacaataa acgcggtgat cgaaagagca atggtcacca cccagaaaat
      181 aatctgggaa accgttgggc gttttatatt ttttagaaat tcaaatctat ccattcactc
      241 attacctcaa ggcttaatat ataccatatc caagcctagt cgttctaaac ccatctgtgc
      301 ccaatacccc aacacagctg agtcttcttg aatacattgc atcatgacag ggatagcacg
      361 atgatcgcgc agttctgaga gtgctcgcaa tgccaaaata cggatgccct gctccccatc
      421 ttttgccgcc tcaataagac ttggtacagc agcttttccg atcttgacta aagcattcgc
      481 cgcaagcccc gctgcaagtg ggtcactatc tctcaggttt tgagccaacg ctgaaacagc
      541 ttcttcgccc gggtgctgag acaatgccaa cgcggcagcg gcacgtacct cagggctgga
      601 atcacttagg gtggaaatca ggtctgaggt ctgggtgtga ggcgaagcgg cgagggcacg
      661 cactgcccac caacgtgtgt ctgtgttttc agagtaggtc aattccaata aggctggaat
      721 tgccgccgct cctgcatcta caatagcggg aatggcttgt tctgcgcgtt catcgtctcc
      781 gcttgtaagc tcagcgaaca ggtctttcat ttttatttat tttgcaggcg gcgggacggg
      841 caacgcatct tgcgcggtgt tcacccattt atcgccttct tcaatcaaca tcacggggat
      901 gtcatccaca atgggatatt ttcgtccgca atcctggcag atgagccagg catctttgtg
      961 gagtttcagc aaaccgtctt tttcgcgcac gcagctgggg cagcgcaaga tttcgagtaa
     1021 ttcagtactg accattgttt ctccttgttg ggaaaagatt gtaccatgta ggttttgaca
     1081 agattttatt tatgaactaa agccacaaac ttcgctcttc cttgcttgtc ttcgcggatc
     1141 ttctgtgccg ggaccatatt gacaaaccgc agcccacggt tcatagtgag tttgaatttg
     1201 gtcggtgaaa tatactgccc cattacgcca taccgtgcgt gtgacagtta catcagcacc
     1261 ttcagcggcg taatctactt gtttaagttc gtttgtcttt aaatctacat tttcttcaaa
     1321 gaccgtttct ggggcaggga ccgtattttg tgctcccgtt gtttcccaag tgaccgaacg
     1381 tccatctgat gtggaataga ttttccacgt aatggtgcgc gcagctacat taacattggt
     1441 ttccatcaac atccagtaag gcgtatcgtt tttgaactta aagtcgacca aggggaaata
     1501 gactgtggcg tccatgccaa ccaagtttcc gtccactgag ccgctttgat ccatttcata
     1561 ataaggcaca cgataggcgt gagaataacg ttccaccact gggtaccctg caaagaacac
     1621 ggtgcggaac aaggtggtgc tcacctgaca cacaccgcct cccacgcctt tgatggtacg
     1681 accgccataa ataatgagtg cttc
//
LOCUS       DAOQNJ010000590         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_64645_length_1703_cov_8.520631, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000590 DAOQNJ010000000
VERSION     DAOQNJ010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64645_length_1703_cov_8.520631"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="PLG52_13265"
     CDS             complement(<1..1693)
                     /locus_tag="PLG52_13265"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNB42469.1"
                     /translation="MAKFTREDALEYHRLKGKPGKVAIVPTKPMDTQRDLSLAYSPGV
                     AEPVLEIEKNPADAYEYTSKGNLVAVISNGTAILGLGDRGALASKPVMEGKGVLFKKF
                     ADIDVFDIEVNSHDPDEIIKVVAAIAPTFGGINLEDIKAPECFYIEEELKKMLDIPVF
                     HDDQHGTAIISSAGLANALEIVGKKHEDIRIVISGAGASAISCAELAIRWGVKRENIM
                     LCDSKGVVYKGRKEGMNKYKERFLIETDARTLSDALRGADVFYGLSVANVMTPEMVKS
                     MAKDPIIFAMANPDPEIRPELAKEARKDVIIATGRSDYANQVNNVLGFPFIFRGALDV
                     RAKQINEDMKFAASKALAALAKEDVPDSVIRAYGGEPIKFGREYIIPTPLDPRVLLWE
                     APAVAEMAMKTGMARKTIDIDEYREQLAFRQGKGERIRYFFQNKARSSGGKKRVAFAE
                     GEEQKIIRAAYQIQEEGIATPVLIGRKDVIEKEIKSLSFDYKPEIVDPNNYFKLEAYA
                     KAFYELRQRKGLTVGDALKKVRDVNVLGSMMVKMGDADAFVSGLTYDYPEVIRPAL"
BASE COUNT          436 a          477 c          347 g          443 t
ORIGIN      
        1 gcaatgctgg gcgaatcacc tctggataat cataggtcaa gccagagacg aaggcgtccg
       61 catcacccat tttcaccatc attgaaccca acacattaac atcgcgcacc ttcttaagcg
      121 cgtcaccaac tgtcaaccct ttgcgttggc gcaattcgta gaacgcctta gcatacgctt
      181 ccagcttaaa gtaattatta ggatctacaa tttcaggctt gtaatcgaag ctaagtgact
      241 taatctcttt ctcgatcaca tcttttcgcc cgatcaacac cggagtagca atgccttctt
      301 cctgaatctg atacgccgca cggatgatct tctgttcttc gccttcagca aatgcaacac
      361 gtttcttacc accagaagaa cgagccttgt tctggaagaa gtaacgaatc cgctcgccct
      421 tgccttgacg gaatgccaac tgttcacgat attcgtcaat gtcaatggtt ttgcgtgcca
      481 tgccggtctt cattgccatt tcagctacag caggagcttc ccacaatagc acacgcggat
      541 caagcggagt tgggatgatg tactcacgcc caaacttgat cggctcgcct ccatacgcgc
      601 ggatgactga atcaggcaca tcttcttttg ccaacgctgc cagcgctttc gacgcggcga
      661 atttcatatc ttcattaatt tgcttggcac gcacatccaa cgcaccacgg aagatgaacg
      721 gaaagcccaa cacattattg acctgattag cataatcgga acgacccgtc gcaataatga
      781 catccttacg agcttctttt gccagttctg gtcgaatctc tgggtcgggg tttgccatgg
      841 caaaaataat cgggtccttt gccatactct tcaccatttc gggggtcatc acattggcta
      901 cagacaagcc ataaaaaaca tcagcaccgc gcaaggcgtc agagagagtt cgtgcatcgg
      961 tttcgataag gaagcgttcc ttatacttat tcatgccttc cttgcgtccc ttgtacacca
     1021 cgcccttact gtcgcacaac atgatgttct cgcgcttcac cccccagcga atcgccaact
     1081 cagcacacga gatggcagaa gcgcctgcac cagaaatcac aatgcgaata tcttcatgtt
     1141 ttttaccaac gatttccaag gcatttgcca aacctgccga ggaaatgatt gccgttccat
     1201 gttggtcgtc atgaaagacg ggaatatcca acattttctt aagttcttct tcaatgtaaa
     1261 aacattcggg ggctttaata tcttcaaggt taataccgcc aaatgtgggg gcaatcgccg
     1321 caaccacttt gatgatctca tcggggtcgt gcgagttaac ttcaatatca aaaacgtcaa
     1381 tatctgcaaa cttcttaaag agaacgcctt tcccttccat tacgggctta cttgccaacg
     1441 ctccgcggtc acccagaccc agaatcgccg ttccattcga gattaccgcc actaaattcc
     1501 ccttggatgt gtattcatat gcatccgcag gattcttctc aatttccaac accggctctg
     1561 ctacaccggg agaatacgcc aaacttaggt cgcgttgggt atccattggc ttggtgggaa
     1621 cgatagccac cttaccaggc ttgcctttca aacgatggta ctcaagagca tcttcacgtg
     1681 tgaatttagc catacagcct cca
//
LOCUS       DAOQNJ010000591         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_65537_length_1690_cov_8.271560, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000591 DAOQNJ010000000
VERSION     DAOQNJ010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65537_length_1690_cov_8.271560"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PLG52_13270"
     CDS             <1..603
                     /locus_tag="PLG52_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNB42470.1"
                     /translation="AHHGRIKEARKEAESRSHRRSIKSKMKEATHYNFVKVGNGRLAL
                     NHRPGNKDYALLRELGCTHVVTLLKESESAKTVGAHTQIAGLEWIWLPIPNGNYPQGD
                     VHERMLVAMPKLSQLLDEGRSLLIHCSAGIHRTGTVAYGLLRWRGMERDEAMQVIHQI
                     RPITAEGMMEKRRRWGDENSRQVGSSTSFWTNLLKRFLKK"
     gene            616..1323
                     /locus_tag="PLG52_13275"
     CDS             616..1323
                     /locus_tag="PLG52_13275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB42471.1"
                     /translation="MFLNSEKYYDAIYGTMGKNYVAEAKIIHELVQKHKRTKGNALLD
                     IACGTGSHAGVLSKYYKVEGLDYDKGMLTVARRKYPNLKFQYGNMIDFSLTRKFDIIT
                     CLFSSIGYARTKANLRKSIKTMADHLVSGGILLVEPWFSPTQWNTGRVHTTHVNNDDL
                     KITRMSFSSKKGNISSIDFHYLIGSSAGVEYFTEKHDLGLFTHEEYMTAFQEAKLKTF
                     HNKKGLDGRGLYVGIKA"
     gene            1320..>1690
                     /locus_tag="PLG52_13280"
     CDS             1320..>1690
                     /locus_tag="PLG52_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42472.1"
                     /translation="MSNQTFRSRIRESIQNPTLQTALDNNTERRLQGKDIAFESIPDW
                     RERRQRAHRIRAEVIEHLEQYLDQFITNAQANGAIVHRAKDAAEAINIVLRISNIEHR
                     KPLVAKSKSMVSEEIELNHAL"
BASE COUNT          531 a          383 c          391 g          385 t
ORIGIN      
        1 gcacatcatg ggcggattaa agaggcaagg aaagaagcag agagtcgttc acatcgcaga
       61 agtattaaat caaagatgaa agaagcaacg cactacaact ttgtaaaagt aggcaatggt
      121 cggctggcac ttaatcaccg ccctggcaat aaagattatg ccctgcttcg tgaattaggt
      181 tgtacgcatg tagtaacctt attgaaggaa agcgaatctg caaaaacagt gggagcccac
      241 acccagattg ccggactgga atggatttgg ctgcccatcc caaatgggaa ttatcctcaa
      301 ggtgatgttc acgaacggat gctcgtcgcc atgccaaaac tctctcaact gctggatgaa
      361 ggcaggtctt tgctcattca ctgctcggca ggaatccatc gcacaggcac ggttgcttat
      421 gggttacttc gctggcgcgg catggaacgg gatgaagcca tgcaggtcat tcaccagatt
      481 cgcccgatca cagccgaagg catgatggaa aaacgcaggc gttggggcga tgaaaactcg
      541 cgacaagttg gatcatctac atctttttgg acaaatctac tcaaaagatt tttgaaaaaa
      601 taagaggcag acaccatgtt cttaaattca gaaaaatact acgatgcaat ctacggtacg
      661 atgggcaaga attatgttgc agaagcaaaa attattcatg agcttgttca aaagcacaaa
      721 cgaaccaaag gaaacgcctt attggatatt gcctgtggaa cagggtctca tgccggtgtt
      781 ttaagcaaat attacaaagt cgaagggttg gattacgaca aagggatgtt gaccgttgcc
      841 cgtagaaagt acccgaattt gaagtttcaa tacggcaaca tgatcgattt ttctttgacg
      901 cggaagttcg atatcatcac ctgtcttttc agctcaatcg gctacgcccg gacaaaagcg
      961 aatctccgca aatcaattaa gaccatggca gatcatcttg tctccggtgg catattatta
     1021 gtggaaccat ggttttcacc gacccaatgg aatacaggac gagttcatac tactcatgtg
     1081 aacaatgatg acctaaagat cacacgtatg agtttcagta gcaaaaaagg gaatatatcc
     1141 agcatcgact ttcattacct gatcgggagt tcagccggag tggaatattt caccgaaaag
     1201 catgatctgg gtttattcac acacgaagaa tatatgaccg catttcaaga agcgaagcta
     1261 aaaacattcc ataacaaaaa aggcttggat ggacgcggtt tatacgttgg aattaaagca
     1321 tgagcaatca aacgttccgt tcacgcattc gtgaatcgat ccagaacccg actctccaaa
     1381 ctgcgttaga caacaacacc gaacgccgtt tgcagggaaa agatatcgca tttgaatcca
     1441 tccccgattg gcgcgagaga cgtcagcggg cgcatcgcat ccgtgcggag gtcattgaac
     1501 atctggagca atatctcgat caattcatca ccaacgccca agcgaatggt gcaatcgtcc
     1561 accgtgccaa agatgcagcc gaagccatca acatcgtatt acgaatatcg aatatcgaac
     1621 atcgtaaacc gcttgtcgcc aaatccaaaa gcatggtcag cgaagagatc gaactgaatc
     1681 acgcgctcga
//
LOCUS       DAOQNJ010000592         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_65737_length_1687_cov_10.441789, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000592 DAOQNJ010000000
VERSION     DAOQNJ010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65737_length_1687_cov_10.441789"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PLG52_13285"
     CDS             complement(<1..1076)
                     /locus_tag="PLG52_13285"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB42473.1"
                     /translation="MMSSSRLNYGKTFLLGFGFFGVSVIWGVYNAFVPIFLSEKFNLS
                     PVLIGFFMTLDNIAALFIQPPVGAWSDKLRTPIGRRIPFILIGAPITALAFGLIPIAA
                     VLPLFVACTSTLLLSAALWRTPVVALMPDITPSEFRSQANGIINFMGGIGTIIALQTG
                     GILYKMSPSFPFWLGSGLVLIAALIVYLFIEEPKEYEAAEEQPGMFASLKEVVSDPDK
                     SGLRILLAIFFWFIGFSAVETFFTLYAKNHLGLNEGDGATLLSVLPLFFVLAAIPSGY
                     VAGKLGRKTTIATGLIIITIMLVLLYITPAEALLTGIAPLPLVGIPLVEGGARMLTLA
                     GVLLIIGGIGWAFININSLPMIVD"
     gene            complement(1133..>1687)
                     /gene="trmD"
                     /locus_tag="PLG52_13290"
     CDS             complement(1133..>1687)
                     /gene="trmD"
                     /locus_tag="PLG52_13290"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNB42474.1"
                     /translation="EAIETVLGLASPLSTPEPESNIPIILLTPQGRVFNQSIAKELSQ
                     HKKIALLCGRYEGIDERIRENLVTDQISIGDYVLTGGELPALILIDAVSRLLPNVLGD
                     PTGAEDDSHAMGLLEYPHYTRPPEFRGSKVPDVLLSGDHKKIDKWRREQALLRTLQKR
                     PDMLETAELTKEDKKFLEKLKNQS"
BASE COUNT          455 a          378 c          472 g          382 t
ORIGIN      
        1 aaatccacga tcatgggcag agagttgatg ttgatgaatg cccaaccgat gccgccgatg
       61 atgagcaaaa cgccagccag tgtgagcatg cgtgcgccgc cttccacgag cggaatgccg
      121 accagtggca gaggcgcaat gccggtgagc aacgcttcag caggggtgat ataaagcaag
      181 accaacatga tggtgataat gatcaaacca gtggcaatgg tggttttgcg cccaagcttc
      241 ccagccacat acccagaagg gatggcagcc agcacaaaga acagtggcag gacggagagt
      301 aaggttgcgc cgtcaccttc gttcaagccg agatgattct tggcatagag tgtgaagaag
      361 gtttcaaccg cagagaaacc aatgaaccag aaaaagatgg caagcaaaat gcgcaacccg
      421 cttttatcgg ggtcgctgac tacttctttg agactggcaa acataccggg ttgttcttcg
      481 gcggcttcgt attctttggg ctcttcaatg aaaagatata cgatcaacgc agctatgagc
      541 acgagcccag agcctaacca gaatgggaag ctgggactca ttttataaag tatgccaccg
      601 gtttgcaggg cgatgatggt gccaattccg cccatgaagt tgataatgcc attggcttgc
      661 gagcgaaatt cagagggtgt gatatcaggc attagcgcta ctacaggggt gcgccataaa
      721 gcagcactga gcaagagcgt gctggtacat gccacaaata gcggaagcac tgctgcaata
      781 ggaatcaagc cgaatgctaa ggcggtgatg ggtgcgccga tcaaaataaa tggaatgcgg
      841 cgaccaatgg gcgtgcgcaa tttatcagac catgctccaa caggcggctg aatgaacaat
      901 gccgcaatgt tatcaagggt catgaagaaa ccgattaaca caggggagag gttgaatttt
      961 tcagagagaa aaatgggaac gaaggcgtta tacacacccc agatcacact cacaccaaaa
     1021 aaaccgaagc cgagcaggaa ggtcttgccg tagttcaatc ttgacgatga catcatagcc
     1081 tccaggttgg tttacaggtt ggtaggttta aatgcttaag tctgccaacg ttttaacttt
     1141 gattctttag cttctcaaga aactttttat cttcttttgt gagctctgca gtttcaagca
     1201 tatcggggcg cttttgaagt gtgcgtaaga gtgcttgttc tctgcgccat ttgtcaattt
     1261 ttttgtggtc accagatagg agcacatcgg gcactttgct accgcgaaac tcaggcggac
     1321 gggtgtaatg gggatactcc aacaagccca tggcgtgcga gtcatcttcg gcgccggtgg
     1381 ggtcgccaag tacattgggc aggagacgag aaaccgcatc aattaggatg agcgcgggca
     1441 gttcgccacc agtgagcaca tagtcgccaa tggagatttg gtcggtcacc aagttctcgc
     1501 ggatgcgttc gtcaatgcct tcataacgtc cgcatagcag ggcgatcttt ttgtgttgtg
     1561 ataattcttt ggcaatggat tggttgaaga cgcgtccttg cggcgtaagc aaaatgattg
     1621 ggatgtttga ttcaggctcc ggcgtggaaa gaggcgaagc gagtcccaga acggtttcaa
     1681 tggcttc
//
LOCUS       DAOQNJ010000593         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_65969_length_1684_cov_8.639656, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000593 DAOQNJ010000000
VERSION     DAOQNJ010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65969_length_1684_cov_8.639656"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PLG52_13295"
     CDS             <1..1182
                     /locus_tag="PLG52_13295"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNB42475.1"
                     /translation="LSHFKINSTETVSLIDAANRILGIDILADGDYPPFDNSAVDGFA
                     LRAEDTSASPVTLNVVADIPAGSTPKVTLQAGQAARIMTGAQMPLGANCVIMVEDTDF
                     NAKSAGTTAPKTVSFSKQMKSGENLRLRGADIRTGDAVLKQGRTLKPQDLGLLATLGH
                     AHVTVHKKARVALLSSGDELLDVSAPLEPGKIRDSNSYALAAAVSNLGVDVIRLGVAR
                     DTRESITQLLDQAASENADLILSSAGVSVGAFDYIKEVIDANGKMDFWRVNMRPGKPL
                     AFGEYRGIPFIGLPGNPVSAFVGFEVFVRPVLERLNGKMDGGRQTVRVRCEEEIESDG
                     RESYLRAKIQVKNNIRIAILTGHQGSGNLLSLVQADALLIIPAGVKCVPAGSEVDAIL
                     L"
     gene            1189..1614
                     /locus_tag="PLG52_13300"
     CDS             1189..1614
                     /locus_tag="PLG52_13300"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNB42476.1"
                     /translation="MDKWLYRVMIALVIIGLAVSVYMTIYKISGNDGMCLGSGDCSTV
                     NASRFSEVNGIPVAVFGIVGYAAILIVLFYENKNEFFKRNGTLLAFGMSLTGFLFTLW
                     LVYVELVLLKAICPFCVTSQVSMTLIFIIAVVRLFRNEN"
BASE COUNT          413 a          393 c          431 g          447 t
ORIGIN      
        1 ctttcccact tcaagatcaa ctcaacagaa accgtttctc ttatagatgc ggcaaatcgc
       61 atcttgggaa tagacattct tgcagatgga gactatccgc cgttcgacaa ttcggcagtg
      121 gatggatttg ctctgcgcgc ggaagacacc tccgcctctc ccgtgacctt gaatgtggtt
      181 gccgacattc cagctggatc cacaccgaaa gtgactctgc aagcgggtca agccgcgcgg
      241 atcatgaccg gagcccaaat gccgcttggt gcaaactgtg tcattatggt agaagacaca
      301 gatttcaacg cgaaaagcgc agggacaacc gcccccaaga cagtctcctt ttccaaacag
      361 atgaaatcag gagagaacct tcgtctgcga ggcgcagata tccgcactgg agatgctgtc
      421 ctgaaacagg gtcgcactct caaaccgcaa gacttgggtc tgctcgccac gcttggacat
      481 gcacatgtaa cggtccacaa gaaagcgcga gtggcattgc tatcctctgg agatgagtta
      541 ttggatgtaa gtgctccgtt ggaacccggc aagatccgtg actctaattc ctatgccttg
      601 gcagccgcag tcagcaacct cggcgtagat gtcattcggc tgggtgttgc aagagatact
      661 cgcgaatcta tcacccaact tttggatcaa gccgcaagtg aaaacgcaga ccttatcctt
      721 tcctctgccg gggtgagtgt aggcgctttt gactacatca aagaagtgat cgatgccaac
      781 ggcaaaatgg atttttggcg ggttaacatg cgcccaggca aaccattggc atttggcgaa
      841 tatcggggaa ttccgttcat tgggctgccg ggcaaccccg tttcagcctt tgttggcttc
      901 gaagtttttg tgcgccccgt gcttgaacgg ctgaacggca aaatggacgg aggcaggcaa
      961 acggtccgcg tacgatgcga agaagaaatc gaatcagatg gtcgcgagag ttatttgcgg
     1021 gcaaaaatcc aagtcaaaaa taacattcgt attgcaattt tgaccggaca ccaaggctcg
     1081 ggtaaccttt tgtcactagt acaggcggat gctttactaa tcatccccgc tggtgtaaaa
     1141 tgcgtgcctg ctggttcgga agtagacgct attctattgt gaggaaccat ggataaatgg
     1201 ctttatcgcg tgatgatcgc gcttgtaatc atcggtttgg cagtgtcagt ttatatgaca
     1261 atttataaga tcagtggaaa cgatggcatg tgcctcggct ctggtgattg ctccactgtg
     1321 aatgccagtc gcttttcaga agtgaacgga attcctgtag cagtatttgg tattgtcggc
     1381 tatgctgcca tcttaatcgt tttgttctac gagaacaaaa atgaattttt caaaaggaac
     1441 ggaaccttgc ttgcctttgg catgtcactt actggtttct tgttcacgct ttggttggta
     1501 tatgttgaac ttgtattgct caaagcgatc tgcccgttct gcgtcacctc gcaagtctct
     1561 atgacgttga tctttattat tgcggttgta cgactattcc gaaacgagaa ttaggaggat
     1621 tcgaaatgcc ccgtattaaa tgtcactatg ttgattgtgt ctttttggat gaaggattgt
     1681 gcgg
//
LOCUS       DAOQNJ010000594         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_65971_length_1684_cov_7.681400, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000594 DAOQNJ010000000
VERSION     DAOQNJ010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65971_length_1684_cov_7.681400"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..1269
                     /locus_tag="PLG52_13305"
     CDS             337..1269
                     /locus_tag="PLG52_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42477.1"
                     /translation="MTDEPIKEDPVQKFRKLIASFIPLSRLPKKSTQAEPKEELPVEE
                     IKEEVKVEENKKDALSALPKKSTPQNDMLGSLPKKGALPPKKAEPKPVETPAPAPSPA
                     STPTSKLVPASSSGYSVWGPRLWTTASVISLTINIILMIVLIILVISVYRLKLDMAKI
                     MDASTSVMGLPAGLYSNFEKMERASIQTNVEVNTSIPVKFDLQLNQQTNVVLSQDVTI
                     QNARVTVNTGGLNISQANTTIVLPAGTTLPVNLALTVPVDQQVPVTLNVPVDIKLKDT
                     QLNDPFVGLQEVIKPLYCLLEPTAVNMDGNPICQ"
     gene            1329..>1684
                     /locus_tag="PLG52_13310"
     CDS             1329..>1684
                     /locus_tag="PLG52_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNB42478.1"
                     /translation="MHKNITQVQLKNGMKVMLKEIHTAPIISSWIWYKVGSRDESTGK
                     TGISHWTEHMMFKGTKKFPGGILDKAISREGGRWNASTSRDSTRYYETMPAAKIDLAL
                     RLESDRMTKSIFDAKEV"
BASE COUNT          508 a          461 c          334 g          381 t
ORIGIN      
        1 caacgcgcac tccgctattt gctaattcac gtgccccgaa aagtcctgcg ggtcccgcgc
       61 caatcacggc gacaaaaaat tgactctcca cgttcttcct ccgaaataaa aaaaattaca
      121 ttttagacaa aaactatcag gttttaccac aaacatcaca gaatttacag acaaaaaacc
      181 gttcaattct gttatcacag tcgaatgcta cctgcaaaaa gttacttttc agatgttaat
      241 tccgcattag agatgtttgt gcttgctgta agaaacccac cccatcttca taaacatcta
      301 tgctaatatt aatccatgtg ctaaccaaga ggcagtatga cagacgaacc catcaaagaa
      361 gatcctgtac aaaaattccg aaagcttatt gctagtttca ttccactctc acgcctgccc
      421 aaaaaatcaa cacaggcaga gcctaaagaa gaactacccg tggaagaaat aaaagaagag
      481 gtcaaagtgg aagaaaataa aaaagacgct ctttcagctt tacccaaaaa atcaacgcca
      541 caaaatgaca tgctggggtc gttacccaaa aagggagcgt taccgcctaa aaaagctgaa
      601 ccgaaaccgg tagagactcc ggcgcccgcc cccagccccg cctcaacccc aacctcgaaa
      661 ctggtacctg cttcaagtag cggatactcc gtttggggtc ctcgtttatg gacaacggca
      721 agcgttatct ctcttacgat caacatcatt ctgatgatcg ttcttatcat tctggtgatc
      781 agcgtctatc gtctcaaact agatatggcg aagatcatgg acgcttccac ctccgtgatg
      841 ggacttcccg ctggcttata ttccaacttc gaaaagatgg aacgcgccag cattcagacg
      901 aatgtagagg tcaacacctc gattccggtc aaatttgact tgcaactcaa ccaacaaacc
      961 aatgtcgtct tgagtcaaga tgtgaccatt caaaacgcgc gtgtcaccgt caacaccggc
     1021 gggttgaata tttcgcaagc caacaccacc attgtcctcc cggcgggcac tacattacct
     1081 gtcaacttag cccttaccgt ccccgtagat cagcaagtgc cagtgacctt gaacgtccct
     1141 gttgatatca agctgaaaga cacgcagttg aacgacccct ttgttggctt acaagaagtc
     1201 atcaaaccgc tttactgttt gctcgaaccc accgccgtca atatggatgg caacccgatc
     1261 tgccagtaat acttgcttgt tgtgcaccgt ctgaggatgc aaaataacag aaaatacttt
     1321 ggagttaaat gcataaaaac atcacgcagg tccagcttaa gaatggcatg aaggtcatgc
     1381 tcaaagagat ccatacggca cccatcatct cgtcgtggat ctggtacaaa gtcggctcgc
     1441 gcgatgagtc caccggcaag accggcatct ctcattggac agaacacatg atgttcaaag
     1501 ggacgaagaa atttccaggc ggtattctcg ataaagccat ctcccgtgaa ggcggacgtt
     1561 ggaacgcatc cacttcccgc gattccaccc gctattacga aaccatgccc gccgccaaaa
     1621 ttgaccttgc cctgcgcctc gaatcagacc gcatgaccaa gagcattttc gatgccaaag
     1681 aagt
//
LOCUS       DAOQNJ010000595         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_66182_length_1681_cov_6.062731, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000595 DAOQNJ010000000
VERSION     DAOQNJ010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66182_length_1681_cov_6.062731"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLG52_13315"
     CDS             complement(<1..382)
                     /locus_tag="PLG52_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42479.1"
                     /translation="MDTILQVFQAVGYLLLLVLLLVLWRKFVQRQEVRAFWFLLALAL
                     TINLLGNIAWIIHDMVTGRSLGTFSGIDLFYVSRYVLIGIALWLYPLSLPRKAGIWTG
                     IVTVMVTVLIWVVYFPPAMKLRGGD"
     gene            complement(455..1138)
                     /locus_tag="PLG52_13320"
     CDS             complement(455..1138)
                     /locus_tag="PLG52_13320"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HNB42480.1"
                     /translation="MLSITVTEAWKSAHPGAAIGLLEVSGVDNTIASAELEVRKREVE
                     AQLREKYQNYTRQDFLSIPVIQAYVQYYKRFSKTYHVLQQVESIALKNKNLPTVSPLV
                     DANFMAEVNTLALAAGHDVSKLRGSILIDVAQNGDQMTQMNGASKEIYEGDMVMKDEH
                     GICCCIIYGQDNLSPISPETTHALYVVYTPVGVPVESVEAQLRAIEANIRLFTPSLVV
                     EQSRVLTNS"
     gene            complement(1143..>1681)
                     /locus_tag="PLG52_13325"
     CDS             complement(1143..>1681)
                     /locus_tag="PLG52_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNB42481.1"
                     /translation="LMDAGRLPDTVIACVGGGSNAIGVFSGFVNDEAVELIGVEAGGS
                     GIASGKHAARFGDVTKARVGVIHGTRTYVLQDGDGQIAETHSVSAGLDYAAVGPEHAM
                     LRDNERAFYTSATDEEALNAFQTLCHTEGIIPALESSHAIAEVIKKAPTMRKDQVILV
                     NLSGRGDKDLNTVIKELK"
BASE COUNT          457 a          439 c          360 g          425 t
ORIGIN      
        1 catctccacc gcgaagcttc atagctggcg ggaaatacac tacccagatc aatacagtga
       61 ccattaccgt cacaatacct gtccaaattc cagctttgcg aggcaaagat aaaggatata
      121 accacagtgc aatgccaatc aatacataac gagatacata aaacagatca attccagaga
      181 atgtgcctaa agacctgccg gtcaccatgt catgaatgat ccatgcaatg tttccaagca
      241 gattaattgt gagtgctaaa gcaagcaaaa accaaaatgc gcggacttct tgtcgctgta
      301 caaactttct ccaaagaacc agtaaaagta ctaacagcag taaatatcct actgcttgaa
      361 aaacctgcaa aatcgtatcc attttttctc cgttgcgaat ttacaccgtg cgcaaagtaa
      421 attctaacca tttttcgctt tacaaaagcc actttcaaga attggtgagc acacggcttt
      481 gttctacaac aagagaaggt gtaaacaaac ggatatttgc ttcaatcgct cgtaattgcg
      541 cttccacaga ttcaacggga acacctacag gtgtatacac gacatacaac gcatgagtag
      601 tctctggtga aataggcgaa agattatcct gcccatagat gatgcagcaa caaattccat
      661 gctcatcctt catcaccatg tcgccttcat atatctcttt cgaagcgccg ttcatctggg
      721 tcatctgatc accattttga gctacatcga tcaagatcga gcctcggagc ttggacacat
      781 catgtcctgc cgccaatgca agcgtattca cttctgccat gaaatttgca tcgaccaacg
      841 gcgagacggt tggcaggttc ttgttcttca aagcgatcga ttcgacttgt tgcaacacat
      901 gatatgtttt actaaaccgt ttgtaatatt gaacataggc ttggatgaca gggatggata
      961 aaaagtcttg ccgtgtatag ttttgatatt tctctcgaag ttgcgcctcg acctctcgct
     1021 tgcgaacttc taactctgcc gaagcaatcg tgttatctac gccagagact tccagcaagc
     1081 cgatcgcagc gccagggtgc gcagacttcc atgcttctgt tacggtgatg gacaacatag
     1141 gattacttca attccttgat gacggtattc aagtctttat cgccgcgtcc cgacaggttc
     1201 accaaaatca cctgatcttt gcgcatggtt ggcgctttct tgatgacctc tgcaattgca
     1261 tgagaggatt ccaacgcggg gatgattccc tccgtatggc acaaggtttg gaaagcgttc
     1321 aatgcttcct catctgttgc ggaggtgtag aaggcgcgtt cattatcgcg cagcatggcg
     1381 tgttcgggac cgacagcggc atagtccaaa ccagccgaga cagaatgagt ctcagcaatt
     1441 tgaccatctc catcttgcag gacataagtg cgagttccat gaattactcc aacccgtgcc
     1501 ttggtcacat caccaaagcg agccgcgtgt ttgcctgagg cgattccgct gccgcctgct
     1561 tccacgccga tcaattccac ggcttcatcg ttcacaaatc cgctgaagac gccgatggca
     1621 ttcgacccgc cgcccacaca ggcgatgacc gtatcgggca ggcgtcccgc gtccatgagc
     1681 a
//
LOCUS       DAOQNJ010000596         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_66254_length_1680_cov_8.580308, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000596 DAOQNJ010000000
VERSION     DAOQNJ010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66254_length_1680_cov_8.580308"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLG52_13330"
     CDS             complement(<1..332)
                     /locus_tag="PLG52_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42482.1"
                     /translation="MNQPIQFKQGIGRWAGNAEVFDSKGCFLGNGSDYRNVQALGSGR
                     VRIDVSFVGPFKHSGHYFIQEEGDHRLYEGPANIGYAETLSENLVDANAYWSALGLSQ
                     RFFLMIVDG"
     gene            complement(373..1188)
                     /locus_tag="PLG52_13335"
     CDS             complement(373..1188)
                     /locus_tag="PLG52_13335"
                     /inference="COORDINATES: protein motif:HMM:NF024430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HNB42483.1"
                     /translation="MIIQTTLEGKPNFAIEQPDHTRTCGQLARAFGNADFAQPTPRDL
                     VVYIVEHHDEGWAPIDALREQSPTTGLPHHLTQTPIQYLIQTSKGSPEFNEKHHAYCG
                     LLSSMHTYGLFNGRYGLSDFIFIDKISAEHKASADAMLAYELARQARLKADLGADATS
                     QAWIEEKALFNNYKLLQFFDTLALYFHTTHAEGRKEGKFLNVPDGKGTDHTVSITPRE
                     DGSYVLSPWVFEGDSLEVSVEGRYITAQPQGTDFKAIFDNTPKEKQIYKLVRE"
     gene            complement(1280..>1680)
                     /locus_tag="PLG52_13340"
     CDS             complement(1280..>1680)
                     /locus_tag="PLG52_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42484.1"
                     /translation="RWEGLCKTFSPKGDFIESAAVHMDVYWNEIGKSWHLMEDFENLY
                     EVGKTVFHSDISTDGKYCWATSEQLSLHGSELTPYNYVFTIDSNISKTLVHNNHYFID
                     PNNRRIITHKLREGKTHIFQIQDFVRIQQP"
BASE COUNT          386 a          386 c          458 g          450 t
ORIGIN      
        1 ccatccacga tcatcaagaa gaaacgttga ctcaacccaa gcgcagacca gtaagcattc
       61 gcgtccacca aattttcgct gagcgtttca gcgtagccga tgtttgcagg accttcgtac
      121 aaacgatggt cgccttcttc ttgaatgaag taatgtccgc tatgcttgaa gggaccaaca
      181 aaggacacat caatacggac acgtccgctg ccgagcgctt gcacgttgcg atagtccgag
      241 ccgttgccga ggaaacagcc tttgctgtcg aatacttcgg cgttgcctgc ccagcgtccg
      301 atgccctgct tgaattggat gggttgattc atgcgagtga ccagttatca gtttccagta
      361 atcagtgagt ggttattcac gcaccagttt atagatttgc ttctctttgg gtgtgttatc
      421 gaaaatggct ttgaaatctg ttccctgcgg ttgggcagtg atgtaacgtc cttcgacaga
      481 gacttcaagc gagtcacctt cgaagaccca aggagataag acatatgagc catcttctcg
      541 cggggtgatg ctgacggtgt gatctgtccc tttgccatcg ggcacattga ggaatttgcc
      601 ttccttgcgt ccttcggcat gggtggtgtg gaagtacaac gccagcgtgt cgaagaactg
      661 caggagtttg taattattga acaaggcttt ttcttcgatc caggcttggg aggtggcgtc
      721 cgctccgagg tccgctttga ggcgggcttg acgggctaac tcatatgcga gcatcgcatc
      781 cgctgaggct ttgtgctctg cggaaatctt gtcaatgaaa ataaaatccg acagaccgta
      841 gcgcccgttg aacaagccat aggtgtgcat ggacgagagc aagccgcaat aggcgtgatg
      901 tttttcgttg aactcgggcg agcctttgct ggtttgaatg agatattgaa tgggtgtttg
      961 cgtgaggtga tgcggcaagc ccgtagtcgg cgattgctcg cgcagcgcat cgatgggtgc
     1021 ccagccttca tcgtgatgtt cgacgatgta gacgacgaga tcgcgcggtg tgggttgagc
     1081 aaagtccgcg ttgccaaatg cacgtgcgag ttgaccgcag gtgcgggtat ggtcgggttg
     1141 ttcgatggcg aagtttggtt tgccttcaag tgtggtttgg atgatcatcg tatctccata
     1201 actaaccgct aagcacgcaa aaagcgcgaa gaagatcttt agtttttctt ggcgaccttc
     1261 gcgctcttcg cggtgaatat taaggttgtt gaattctcac aaaatcctgg atctgaaaaa
     1321 tatgcgtctt gccttcgcgc aatttatggg tgatgatgcg gcggttgttg gggtcaataa
     1381 agtaatgatt gttatgaacg agggtcttgg aaatattgct atcgatcgta aaaacatagt
     1441 tatacggtgt gagttcggag ccatgcaagc tcaactgctc actggtcgcc cagcagtatt
     1501 ttccgtcagt ggagatgtca gaatgaaaaa cggtcttgcc cacttcatac agattttcaa
     1561 agtcttccat caaatgccaa ctcttgccaa tctcattcca atacacgtcc atgtgaaccg
     1621 cggcagattc gataaagtcg cctttggggc tgaaggtttt gcacaagcct tcccatcgtc
//
LOCUS       DAOQNJ010000597         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_66647_length_1675_cov_9.825926, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000597 DAOQNJ010000000
VERSION     DAOQNJ010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66647_length_1675_cov_9.825926"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLG52_13345"
     CDS             complement(<1..812)
                     /locus_tag="PLG52_13345"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNB42485.1"
                     /translation="MSPKVRNILLGLAGLVLLAVLVYQIPAVKSRLEWRLDVFQIYVK
                     NVIDPVGPVPTALPVTPLPATPTIAITNTAVVQALPTVTATPTFPPLPAQAGITSPPY
                     EKQTANNCGPATLSMALHLYGWEGSQADISNLIKPVSGDRNVNPEELRYYIRTQAGWL
                     NLEYRVGGNLELLKRLLAANYPVIVETVTSLNPADALGPNDDLWAAHYMLITAYDDAK
                     QEFTIQDSYHGPDLSISYKQLEKDWRPFNNVYLLLYFPQYEEEVKTLLGSDW"
     gene            992..1483
                     /locus_tag="PLG52_13350"
     CDS             992..1483
                     /locus_tag="PLG52_13350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNB42486.1"
                     /translation="MTLDPEALRSAMRAWSAGVTVVTAVHEGEMHGMTVNSFTSISLT
                     PPLLIISLQQNTRTHELVMKSRAFGLTILSSEQSKVSDLFAGRMPEVEDRLATLKTET
                     LVTGSPLIVGGLAWLDCRVLQTYDAGSATLFIAEAVSARGTGSGDPLIYHNREYWKLS
                     KLK"
BASE COUNT          418 a          369 c          428 g          460 t
ORIGIN      
        1 tcccagtctg agccgagaag ggttttcact tcctcttcat attgtgggaa gtacagcaat
       61 aagtaaacgt tgttgaaagg acgccaatcc ttttcgagct gtttgtaaga aatactaagg
      121 tctggaccgt gataagaatc ttgaatggta aattcttgtt tcgcgtcatc atatgcggtg
      181 ataagcatgt aatgcgctgc ccataaatcg tcattgggtc cgagggcatc ggcagggttc
      241 agcgatgtga ccgtttctac aatgaccggg taattcgcgg caagcaggcg tttaagtaat
      301 tcgagatttc cgcccacgcg atattcaagg ttgagccacc ctgcctgtgt gcggatgtaa
      361 tagcgcaatt cttctgggtt gacgttgcgg tcgcctgaga cgggtttgat cagattcgaa
      421 atatcagcct gactgccttc ccatccatac agatgaagtg ccatcgaaag tgtggctgga
      481 ccgcagttgt tggcagtttg cttttcatat ggtggtgatg tgataccggc ttgtgctggc
      541 aaaggcggga atgtaggtgt tgctgtgact gttggtaagg cttgcaccac ggctgtattg
      601 gttatagcaa tcgtcggtgt agcgggcaat ggcgtgactg gcaatgcggt agggacaggc
      661 cccactgggt cgatgacgtt ttttacataa atttgaaaca catccaaacg ccattcaagg
      721 cgggatttaa cagcaggaat ttggtaaacc aacacagcta acagtaccaa acctgccagc
      781 cctagtagga tatttctgac ttttggagac atgcgcgtga gtgtaccacg caagcataaa
      841 ggaagattaa ggtaagtttt tgttttgcca ttgaatcaat gtcttgcaaa gagtattcaa
      901 aattgctgaa actatgcggt ttttggcttg acttgctctt ttacggcaat tatgatgaaa
      961 acgagaaata tctatggaga attcccctgt tatgacccta gaccctgaag ccctgcgatc
     1021 tgccatgcgt gcgtggtcgg caggtgtcac tgtggttact gctgtgcacg aaggcgaaat
     1081 gcatggcatg accgttaatt cattcacatc tatttcgttg accccgccct tgctcattat
     1141 ttctttgcaa cagaatactc gtacgcatga gctggtgatg aaatcgcgcg cctttggttt
     1201 aactattctt tcttctgaac aatcgaaggt ctcggatttg tttgccgggc gcatgcccga
     1261 agtggaagat cgcctagcaa cgttgaagac cgagactctt gtaaccggct ctcccctgat
     1321 cgtaggcgga ctcgcctggt tagattgccg cgtcctccaa acttatgatg cgggttcggc
     1381 tacattattt attgccgaag cagtttcggc tcgtggaact ggcagcggcg accccttgat
     1441 ctatcacaac cgtgagtatt ggaaattatc gaaactgaag taaagaaaac gccaagccaa
     1501 aacgcttggc gttctttgta ttcatgcgag gatgccatgt ccgctttgtt gacagataag
     1561 gaaagacaaa ctcttttgca cattgccaga gtgtctattg aaaatgccgt aaagggaaac
     1621 cctcaatcac ctcttgaaaa agatacgctc acacctgctt tgcgtgaaaa cggag
//
LOCUS       DAOQNJ010000598         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_66882_length_1672_cov_6.294991, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000598 DAOQNJ010000000
VERSION     DAOQNJ010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66882_length_1672_cov_6.294991"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLG52_13355"
     CDS             <1..334
                     /locus_tag="PLG52_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42487.1"
                     /translation="SVVGPEVLTGPMGEVLGLRDSQFIQHLQEVDKLYIAGQAKSHCV
                     AWTAQDLLDDILATDPELAKKVYLLDDCSSAVVVPGVVDHTEAANEAYVRFAKVGMHI
                     VKSTDEIR"
     gene            337..888
                     /locus_tag="PLG52_13360"
     CDS             337..888
                     /locus_tag="PLG52_13360"
                     /inference="COORDINATES: protein motif:HMM:NF014001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HNB42488.1"
                     /translation="MANPTRLSKFISLVLRHRANDFGLHLDEQGFVAFEDLKRLVEEK
                     STDVYSDEDWRKVLNGELDGKKRFEMKDGRIRALYGHSKVERVTYEPVVPPDILYHGT
                     TPQAEKAIRREGLRSMKRQYVHLSANTERALNVGGRRTDNVRLLIVRAREAQQAGIEF
                     YSPEPEHYLAQNIPPQFIDFPKE"
     gene            895..>1672
                     /locus_tag="PLG52_13365"
     CDS             895..>1672
                     /locus_tag="PLG52_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNB42489.1"
                     /translation="MSIFDGKRLTNETFKLDIERMRRGWYSDKYFENINRMLTALSKE
                     GYFYSGNHHNLPEGMSPEQIAVGDIEVEMQWFTRRMGKTTVVGVDKSLEMLKHCSGYF
                     DGDEFMDTSGKLEVWAVQDGTTVKYDGDPSKIQPVIKVRGRYRDFALLETPTLGILTR
                     SSRVATNVYETLVAAKGKPVLFFPARFDVHEVQAADGYAYNIALQRFNKDHAQELGAF
                     VSTDAQGDWWGGAGGGTVAHAAIASFLGDTAEAMMQFSHIL"
BASE COUNT          419 a          326 c          501 g          426 t
ORIGIN      
        1 ctcagtggtc ggtcccgaag tgttgaccgg tccaatgggg gaggtgttgg gtttacgtga
       61 ctcgcaattt attcagcatc tgcaagaagt ggataagctc tacatcgccg ggcaggcaaa
      121 gagtcactgt gtggcatgga cggcgcaaga tttactggat gatattctgg caaccgatcc
      181 cgaactggcg aagaaggtct acctgctcga cgattgttcg tcagcggtgg tggtgcctgg
      241 ggtggtggac cataccgagg cggcgaatga ggcgtatgtc cgctttgcca aggtggggat
      301 gcatattgtc aaatcgacgg atgagatcag gtagagatgg caaacccaac aaggctttca
      361 aaattcattt cattggtctt gcgtcatcgt gccaatgatt ttggcttgca tttggatgag
      421 caaggatttg tagcctttga agatttgaag agattggtcg aagaaaaatc tacagatgtt
      481 tatagcgatg aagattggcg caaagtgttg aatggcgagt tggatggtaa gaagcgtttt
      541 gaaatgaaag acgggcgcat ccgtgcattg tatgggcata gcaaagtgga gcgtgtgaca
      601 tatgaaccgg tagtgccgcc ggatattttg tatcatggca caactccaca agctgagaaa
      661 gccatccgca gggaagggtt gcgctcgatg aagcgtcagt atgtgcatct ctctgcaaat
      721 acggagcgag cattaaatgt gggtgggcgt cgaacggata atgtgcgctt gttgatcgtt
      781 cgcgcaaggg aggcgcaaca agcaggaatt gagttttatt ctcctgagcc ggaacattat
      841 ctggcgcaga atattccgcc ccagtttatt gatttcccaa aggaataaaa agatatgtcc
      901 atttttgacg gtaaacgatt aacgaacgag acttttaaat tagatatcga gcgtatgcgc
      961 cgcggctggt attcggataa atactttgag aatatcaatc gaatgttgac ggcattatcc
     1021 aaggaaggat acttctattc aggcaatcat cacaatttgc ccgaaggcat gtctcctgag
     1081 cagatcgctg tgggggatat tgaagtggaa atgcagtggt ttacacgccg catgggtaag
     1141 acgactgtag tgggggtgga taaatcgctg gagatgctga agcattgctc tggctatttt
     1201 gatggcgatg aatttatgga tacatctggt aagctggaag tatgggctgt gcaggatggg
     1261 acgacggtca agtatgatgg cgacccgagc aagattcaac cggtcatcaa agtgcgcgga
     1321 cgctatcgcg attttgcgtt gttggaaaca cccactttgg gaatcctcac tcgttcaagt
     1381 cgtgtagcca caaatgtgta tgagacgttg gtagcggcaa aaggtaagcc ggttttattc
     1441 tttccggcgc gttttgatgt gcatgaagtg caagccgctg atgggtatgc atataacatc
     1501 gccttgcagc gtttcaataa agaccatgca caggaattgg gcgctttcgt ctctaccgat
     1561 gcgcagggag attggtgggg cggcgcaggc ggcgggacgg tggctcatgc ggcgattgca
     1621 tctttccttg gcgatacggc agaggcgatg atgcagttct cgcacatttt gc
//
LOCUS       DAOQNJ010000599         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_67724_length_1661_cov_11.595268, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000599 DAOQNJ010000000
VERSION     DAOQNJ010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67724_length_1661_cov_11.595268"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLG52_13370"
     CDS             <1..663
                     /locus_tag="PLG52_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB42490.1"
                     /translation="RLHGLHIAKTKEEAEGAEAQEIKERFERVCKDAGVQGSLAIDVG
                     DITQRIIERAVVTDLIIVKLLNPPGSGLSVLNSPFRAIIEKSSRPLITVPKGATPFKR
                     AMLAFDGSDLAKEALFVTAYLAEMWKTEVTVFTAREEKSPQGDLQDYARRYMELHEVE
                     AKYVVSEQKTPRHLPDIAAEYNADLILMGSHSGNKLQQVLVGSMVDITLRESSIPAFI
                     CR"
     gene            745..>1661
                     /locus_tag="PLG52_13375"
     CDS             745..>1661
                     /locus_tag="PLG52_13375"
                     /inference="COORDINATES: protein motif:HMM:NF020028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42491.1"
                     /translation="MTVSASRWDLTNVYPSLDSKQFKAATKKYKSLLDELDAFLKKAE
                     KANSKTEPKKLGKLLGEAVDRFNELFELSGTMSSYLYSFITTDSRNKDAMRIFSEFQQ
                     MSVQGSILQTKFTAWAGTLGKPAIKKAVKTNASAKAHEFILLESAEQSKYLMSEAEET
                     LAAELTLSGGSAFGKLQGTLTSQMTVDFELDGEVKKMPMPALINLRSHPDESVRRRGY
                     EAENKAWADVQETLAACLNGVKGETLVLDRKRGREDNVHASIDAARIDRATLEAMLGA
                     MRDSFPMFRRYFNHKAKLIGKEKLAWWDL"
BASE COUNT          446 a          453 c          405 g          357 t
ORIGIN      
        1 aggctgcacg gtttacacat tgcgaaaaca aaagaagaag ccgaaggggc tgaagcgcaa
       61 gaaatcaaag aacgctttga acgagtctgc aaagatgcag gtgtacaagg cagcctcgcc
      121 attgacgtgg gcgacattac gcaacgcatc atcgaacgtg ccgttgtcac agacctgatc
      181 attgtcaaat tgctcaaccc gccagggtcg gggttatcgg ttttgaactc gcccttccgt
      241 gccattattg aaaaatcatc ccgcccgctt atcaccgtgc ccaaaggcgc cacacctttc
      301 aaacgagcca tgcttgcttt tgatgggtct gaccttgcca aagaagcact cttcgtcact
      361 gcttatcttg ccgaaatgtg gaaaaccgaa gtgaccgtgt tcactgcccg cgaagagaaa
      421 agcccgcaag gcgatttgca agattatgcc cgccggtata tggaattgca tgaagtggaa
      481 gccaagtatg tggtgagtga acaaaaaact ccgcgccacc tgccagatat tgccgctgaa
      541 tacaacgcag acttgatcct aatgggcagc cacagcggaa acaaactaca acaggtccta
      601 gtcggcagca tggtagatat cactttgcgc gagtcgagca ttcctgcttt catttgccgc
      661 tagggtttaa tccctaccag agcaggggga aggtaaaaat caatcacggt atactaagcc
      721 catctttcaa caaggagctt tcgaatgact gtttctgcct ctcgttggga cctaaccaac
      781 gtctatccgt ctcttgattc aaagcaattt aaagccgcca ccaagaaata taaatccctg
      841 ctcgatgagc tggatgcctt tctcaagaaa gccgagaaag ccaactccaa gaccgagccg
      901 aaaaagctcg gcaaattgct cggcgaagct gtagaccgct tcaatgagct atttgagctt
      961 tctggcacca tgagttccta tctttactct ttcatcacaa cggactcacg taacaaagac
     1021 gccatgcgca ttttctctga gttccagcag atgagcgtgc aaggcagcat tttacaaacc
     1081 aagttcactg cctgggcagg gacacttggc aagcccgcca tcaaaaaagc ggtaaagacc
     1141 aatgcttctg ccaaagcgca tgaattcatc ctgcttgaat cggcggagca atccaaatac
     1201 ttgatgagcg aagccgagga gaccctcgcc gccgaattga ccttgagcgg tgggagcgcc
     1261 ttcggcaaac tgcaagggac gcttacctcc caaatgaccg tggactttga gctggacggc
     1321 gaagtgaaga agatgcccat gccggctctc atcaacttgc gctcacaccc cgatgaatcg
     1381 gttcgccgcc gcggctacga agccgagaac aaggcttggg cagacgtgca ggaaactctc
     1441 gccgcttgtt tgaatggcgt caaaggtgaa accctcgttc tcgatcgcaa acgcggacgt
     1501 gaagataacg tccacgcttc catcgacgct gcccgcattg accgtgccac cctcgaagcc
     1561 atgttgggtg ccatgcgcga ttccttcccc atgttccgcc gttatttcaa tcacaaggcg
     1621 aagttgatcg gaaaagaaaa actcgcctgg tgggatctct t
//
LOCUS       DAOQNJ010000600         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_68359_length_1653_cov_9.954318, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000600 DAOQNJ010000000
VERSION     DAOQNJ010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68359_length_1653_cov_9.954318"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /gene="acpS"
                     /locus_tag="PLG52_13380"
     CDS             complement(<1..273)
                     /gene="acpS"
                     /locus_tag="PLG52_13380"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNB42492.1"
                     /translation="MILRTGVDLIETNRIADAIERHGERFLNRVFTAHELSECKGRVP
                     SLAARFAAKEAVAKALGTGIGDVSWLEIEVRSDEKKAPYVVLSGEGE"
     gene            complement(278..1552)
                     /locus_tag="PLG52_13385"
     CDS             complement(278..1552)
                     /locus_tag="PLG52_13385"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB42493.1"
                     /translation="MATKTKSSMPSPEDVYQTTLPNGITVLARSNYNSPSINVGGYLP
                     AGAIFETDEKLGLADFVTAMLMRGTQTRTFDSIYNELETVGASLGFDSGVHNASFGGR
                     ALVEDLPLLMKLLSESLRTPTFPKDEMERLRTQILTGLALRAQDTSDMADLLYEQFLY
                     ADHPYGKPSDGYVETIQAISRKDLETFHRFHFGPRGMVITIVGAIQPKKAVEAVAKFL
                     GDWTVPSQKEPPVLPPLKPLKKTVRKHHQILGKSQSDLVVGTIGPTRKSPDFMAVSLG
                     NSVLGQFGMMGRIGHVVREKSGLAYYAYSSLHSGVGPGSWEVAAGVNPKNLKKALDLI
                     DVELRRFVKNGVTKEELADSQANYVGRLPLSLESNGGMVSAILNIHRYELGMDYYQRY
                     ENMVRSVTRDDVVETARKYIDPERLVIATAGP"
BASE COUNT          405 a          456 c          390 g          402 t
ORIGIN      
        1 ctccccttcg ccagataaaa ccacataggg cgctttcttc tcatccgaac ggacttcgat
       61 ttccagccag cttacatcgc cgattcctgt gcccaatgct tttgccacag cttcttttgc
      121 tgcaaaccgc gccgccaaag atgggacacg ccctttacat tcactcagtt catgcgcggt
      181 aaaaacacgg ttcaggaatc gttcgccgtg ccgttctatg gcatcagcga tacggttcgt
      241 ttctattaag tccacaccgg ttcgcaagat cattactcta cggtcctgct gtagcaatca
      301 ccaaacgctc ggggtcaata tacttgcgag cggtttcaac cacatcatct cgcgtgacac
      361 tgcgcaccat gttttcgtaa cgctggtaat aatccatgcc caactcgtag cgatgaatat
      421 tcaaaattgc gctcaccata ccgccattcg actcaagtga aagcggcaaa cgtcccacat
      481 aatttgcctg actatcagca agctcctctt tagtcacgcc attctttacg aagcgccgca
      541 gttcaacatc aatcaggtca agcgcttttt tcaggttctt cgggttgact ccagccgcca
      601 cttcccaact gcctggtcct accccagagt gcagacttga ataagcataa tatgccaagc
      661 ccgatttttc acgcactaca tgcccaatgc gccccatcat accgaactga cccaagaccg
      721 aattcccaag agacaccgcc atgaaatcag ggcttttgcg tgtgggtcca atcgtgccaa
      781 cgaccaaatc tgattgggat ttgccgagga tttgatgatg cttacggaca gttttcttca
      841 acggcttaag cggaggaaga acaggcggct ctttctgact tggaactgtc cagtcgccca
      901 agaattttgc caccgcctca accgcttttt tcggctggat agcgcccacg attgtaatga
      961 ccatgccgcg aggtccgaag tggaagcgat ggaaagtctc caaatctttg cgcgagatcg
     1021 cttgaatggt ctccacatat ccatcagaag gtttgccata gggatgatcg gcatacagga
     1081 attgctcata gagcaagtct gccatatcag aggtatcttg agcacgcagt gccagcccgg
     1141 tgagaatttg agtgcgcaga cgttccattt catctttcgg gaacgtggga gtgcgcagcg
     1201 attcagagag aagcttcata agaagcggaa ggtcttcaac caaggcgcga ccgccaaaac
     1261 tggcattgtg aacacccgag tcaaagccga ggctcgcgcc gacagtttcc aactcgttgt
     1321 aaatggagtc aaacgtgcgg gtttgggttc cgcgcatgag catggcagtg acaaaatctg
     1381 ccagaccgag tttttcatcg gtttcaaaaa tagcgccagc aggaagatac ccacccacat
     1441 tgatagatgg gctgttgtaa ttggagcgcg ctaaaacagt aatgccattg ggaagtgtgg
     1501 tctggtacac atcctcaggg ctgggcatgg aagacttggt tttagttgcc attacgccgc
     1561 cttccctttg ggaatgtagt tccccaccac acgtttgctc gtctggaaat aaatctgcgc
     1621 cacacgctgc acatctttgg gcgtgacctt ggc
//
LOCUS       DAOQNJ010000601         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_68565_length_1650_cov_9.760502, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000601 DAOQNJ010000000
VERSION     DAOQNJ010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68565_length_1650_cov_9.760502"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..748)
                     /locus_tag="PLG52_13390"
     CDS             complement(38..748)
                     /locus_tag="PLG52_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42494.1"
                     /translation="MLPNLTSQTLPRQELTPEIIQEMFDVFNENFSQTSLDLFTRDLN
                     NKNWVILIRDDEKNIVQGFSTLGIYETEYQGQCITAVYSGDTIIRRAYWGTPELPKKW
                     IHTVLEKTADMPQPVYWLLISSGYKTYRFLTVFYKEFYPCYNQPTPPETQSLMNFLAS
                     QRYGDNFYPDLGVVRFKDGATPLREGVAEVTGERLSDPHVAHYIKVNPHHDQGDELVC
                     LTRVHPDNFTPAGRRMAR"
     gene            complement(790..>1650)
                     /locus_tag="PLG52_13395"
     CDS             complement(790..>1650)
                     /locus_tag="PLG52_13395"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB42495.1"
                     /translation="LPISLLQFGRGCRYVCTFCAVSSYFDHRHFYRSVAQTIEEMKSQ
                     ERNTFFFVDDNIVSDHESAKALFRELIPLKIHWVSQASIDMTRDRELMDLMAKSGCLG
                     NVIGFESIEARSLRSMHKGQNLKGKHGYEAYAEQLEILRDYGMQTWAAFTVGHDYDTP
                     DSILRTLDFALENKFTFAAFNTLLPYPNTALYRKLEAEDRLLYDGKWWLHEEYRYNQA
                     AFRPALMTPDELTEVSYHARKSFNSVGSIFSRAWDFKTNMRNPYRLGVYLVYNPIFRN
                     ETLKKQGIRL"
BASE COUNT          376 a          365 c          494 g          415 t
ORIGIN      
        1 gaagcaaaca accagaggcg gttggcaagt gcggctttca tcgcgccatg cgcctgcctg
       61 cgggggtgaa gttatcggga tggacgcgtg tgaggcagac gagttcgtca ccctgatcgt
      121 gatgcggatt gactttgatg taatgcgcca catgcgggtc ggagagacgt tcaccggtca
      181 cttcggcgac gccttcgcgc aatggggttg cgccatcttt gaagcggacg acgccgaggt
      241 ctggataaaa attatcaccg tagcgttgcg aggcgaggaa gttcatcagg ctttgggttt
      301 cgggcggagt gggctggttg tagcaggggt agaactcttt ataaaaaacg gtgaggaagc
      361 gataggtttt gtaaccagat gagatcagca gccagtagac aggctggggc atatccgctg
      421 ttttttcgag cacggtgtga atccattttt tggggagttc gggcgtgccc caatacgcgc
      481 ggcggataat ggtgtcgcct gaataaaccg ccgtgatgca ttgaccttga tactccgtct
      541 cataaatgcc gagggtggaa aatccctgca cgatattttt ttcgtcgtcg cggatgagaa
      601 tgacccagtt cttgttgttc aggtcgcggg tgaagagatc gagcgaggtt tgggaaaagt
      661 tctcgttgaa gacatcaaac atttcctgaa tgatctcagg cgtgagttcc tgacgcggga
      721 gggtttggct ggtgagattt gggagcatat agattccagt tgtacgtcaa acttaagttt
      781 gccgtacttt tataaacgta ttccttgttt cttgagcgtt tcgtttctga agatcgggtt
      841 atagacgagg taaacgccga ggcggtaagg attgcgcatg ttggtcttga aatcccatgc
      901 gcggctgaag atggagccaa cactgttaaa agatttgcgg gcgtgatatg acacttcggt
      961 caattcatcg ggggtcatca gagcagggcg gaaggcggct tgattgtagc gatactcttc
     1021 atgcagccac catttgccat cgtaaagcag acggtcttcg gcttccagtt ttcggtagag
     1081 ggctgtgttt gggtagggca acagggtatt gaaggcagca aaggtgaatt tgttttcgag
     1141 ggcgaaatcg agcgtgcgca aaatggaatc aggggtatcg taatcgtgcc caacggtgaa
     1201 cgccgcccag gtctgcattc cgtaatcgcg cagaatttcc aactgctcgg cataggcttc
     1261 gtagccatgc ttgcctttca ggttttgtcc tttgtgcatg gaacgcaggc ttctggcttc
     1321 gatggattcg aaaccgatca cgttgccgag acatccgctc tttgccatca aatccatcaa
     1381 ctcgcggtcg cgggtcatgt ctatgctggc ttgcgaaacc cagtggatct tgagcggaat
     1441 caactcgcga aacagtgctt tcgctgattc atgatccgaa acaatgttat cgtccacgaa
     1501 aaagaaggta ttacgctcct ggcttttcat ctcttcgatg gtctgcgcca cactacggta
     1561 gaaatggcga tgatcgaaat acgagctgac cgcgcaaaat gtgcagacgt aacggcatcc
     1621 gcgcccgaat tgcagcaggg agatgggcag
//
LOCUS       DAOQNJ010000602         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_69126_length_1643_cov_12.183879, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000602 DAOQNJ010000000
VERSION     DAOQNJ010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69126_length_1643_cov_12.183879"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="PLG52_13400"
     CDS             complement(<1..1610)
                     /locus_tag="PLG52_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB42496.1"
                     /translation="MIEFKQIVIFLDKMHLFGGLDQNQLSGIANKLVEKEVPAGKEIF
                     KRGEKPDGFYMIFKGKVRVTVPDAEKGERQVAVLYGGDYFGEEALFENRNRSATVRAV
                     EDTTILFLSRQGFDGLLKQYETLKPNFQVSIKSRKLARSTRFKWLGKNEVIYFIARRH
                     KIRLFQALVTPFFSLLGALALIALGFFVSSVIPAAVGVVLVIGVLGWGVWNAIDWSND
                     YYIVTNQRVVWLEKIIFLHDSREEAPLGTILSVGVETDALGRILKYGNVIVRTFVGKL
                     EFEYVDHPQQAADMIREYWERTKAITTRTQMDVMKDTIKQKLGLPLDKRPQVELSPVV
                     PMDEEQVLKTPLWMLALQNLFQLRLEEGGKIIYHKHWIILLQQGWRPFGLFFLGIILF
                     IWRMVVLYNSPDKAFITFNGGFSFKPDTISVVIPLLLLPVAGWVWYEYTDWKNDTFMV
                     TQDEIFDIDRKPFGTEERRSAQIENILSTSYKRKGFIAYMFNYGTVNITVGGTQMAFE
                     DVMDPAAVQADINRRRAQRIAKKNEDAG"
BASE COUNT          438 a          413 c          371 g          421 t
ORIGIN      
        1 ctgccggcat cttcattttt cttggcaatg cgttgagccc gccgacggtt aatgtccgct
       61 tgcacggcag cagggtccat cacatcttca aacgccattt gagtaccgcc tacggtgatg
      121 ttaactgtcc cgtaattgaa catgtaggca ataaacccct tgcgcttgta agatgtgctt
      181 aagatgttct cgatctgagc agagcggcgt tcttctgtgc cgaaaggttt gcggtcaata
      241 tcgaatatct cgtcctgggt gaccatgaag gtatcgttct tccagtcggt gtattcatac
      301 caaacccaac ctgctacagg taataacaaa agtggaatga ccacagagat cgtatcgggc
      361 ttgaaggaaa agccgccatt gaaagtaata aaggctttgt cgggcgagtt atacaacacc
      421 accatacgcc aaataaaaag aataatgcca aggaaaaaga gcccgaatgg acgccaacct
      481 tgctgcaaca aaatgatcca atgcttgtga tagatgatct tgccgccctc ttcaagacgc
      541 aattgaaaga ggttctgaag tgccaacatc cacagtggcg ttttgagcac ttgttcttca
      601 tccatgggca ctacaggaga aagctccact tgcggacgtt tatccaacgg caagcctagc
      661 ttctgcttga tggtgtcttt catgacatcc atttgagtgc gggtggtgat ggcttttgta
      721 cgttcccaat actcacggat catatccgcg gcttgctggg gatgatcgac gtattcaaat
      781 tccagcttgc ccacaaaggt tcgcacgatc acattgccat atttcagaat gcgtcccagt
      841 gcatctgtct caacgccgac ggataagata gttcccaatg gtgcttcttc gcggctatca
      901 tgcaagaaga tgattttctc caaccacaca acacgttgat tggtgacgat gtagtaatcg
      961 ttgctccagt caatagcatt ccacacgccc cagcctaaaa cgccgatcac cagcaccact
     1021 cctaccgccg ccgggatgac agaacttacg aagaagccta aagcgattaa tgccagcgcg
     1081 ccaagcaaag aaaagaatgg tgtgaccaat gcctgaaaga ggcgaatttt atgtctgcga
     1141 gcaatgaagt aaatgacttc gtttttcccc aaccacttaa agcgtgttga acgtgcaagt
     1201 ttgcggctct taatggagac ttgaaaattt ggtttaaggg tttcgtactg tttaagcagc
     1261 ccgtcaaaac cctgacgtga aagaaataga atagttgtat cttccaccgc ccgaaccgtg
     1321 gcagaccgat tgcggttttc aaacagcgct tcttcaccga agtagtcgcc gccgtatagc
     1381 actgctactt ggcgttcacc tttttcagca tctggcacgg tcacacgcac tttcccctta
     1441 aagatcatat aaaagccatc tggcttttcg ccgcgcttaa aaatttcctt cccggcggga
     1501 acttcttttt caacaagctt gttggcaata ccagaaagct gattctggtc caacccgccg
     1561 aagaggtgca ttttatcgag gaagatgacg atttgtttga attcgatcac gttcggattc
     1621 tatcaaactt cacgcacaat ggc
//
LOCUS       DAOQNJ010000603         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_71054_length_1619_cov_6.202046, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000603 DAOQNJ010000000
VERSION     DAOQNJ010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71054_length_1619_cov_6.202046"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLG52_13405"
     CDS             <1..221
                     /locus_tag="PLG52_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNB42497.1"
                     /translation="LAGTPLEVKTEAAHEMVIRLAREEGLFVGISSAAAAVAAIQVAS
                     ELDEGVVVTVFPDAGYKYLSDKTLWEKA"
     gene            222..635
                     /locus_tag="PLG52_13410"
     CDS             222..635
                     /locus_tag="PLG52_13410"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNB42498.1"
                     /translation="MSLKLTNEQVSEIERHIQAAYPGEGAGFLLGVDGEVKGVLPLDN
                     AREEEARYNRFLLTPEDYLKAEMKAMELGVDLIGVFHSHPDCPNVPSEYDREWAQPFF
                     SYIISRVDQGKTVSHRSWRLQEDRSKYDEEQIEVL"
     gene            717..953
                     /locus_tag="PLG52_13415"
     CDS             717..953
                     /locus_tag="PLG52_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNB42499.1"
                     /translation="MDVIKEDQVLDCTGLLCPMPIVKTKKAIDVLESGQVLKMIATDA
                     GSPPDIAAWSRQTGNELLLSTEEGGKFVFFLKKA"
     gene            963..1487
                     /locus_tag="PLG52_13420"
     CDS             963..1487
                     /locus_tag="PLG52_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNB42500.1"
                     /translation="MAKSDPNAPKRLALIASKGTLDWAYPPFILSTAAAAMGWEVGVF
                     FTFYGLTLLKPELTAAVSPLGNPAMPMKMPFGPENFQNISWPMPNLLMANVPGFESLA
                     TNLMKQTFKNKGVASIEELRDMAVELDVRLIGCQMTMDVFGFKKEDFIPQAEIGGAAT
                     FLEYAADADVQLFI"
BASE COUNT          407 a          332 c          457 g          423 t
ORIGIN      
        1 cgctcgcggg cactccgctt gaggttaaga cagaggcggc gcatgagatg gtgattcggc
       61 ttgctcgtga agaaggtctg ttcgtgggaa tctcatctgc agcggcagca gtggctgcga
      121 ttcaagttgc cagtgaactg gatgaaggtg tagtggtgac ggtcttcccg gatgctgggt
      181 ataaatattt gagcgataaa acactttggg agaaagcgta aatgagttta aagttgacaa
      241 atgaacaggt aagcgaaatt gaaagacata ttcaagcggc atacccgggg gagggcgctg
      301 gctttttgct gggcgttgac ggagaagtga aaggggtttt gcctttggat aatgcacgtg
      361 aagaagaagc gcgctataac cgttttttac ttacgcccga agattatttg aaagcggaaa
      421 tgaaagcgat ggagttgggt gtggatttga tcggtgtgtt tcattcgcac cccgattgtc
      481 cgaacgtccc ttcggagtat gaccgtgaat gggcacaacc atttttctct tacatcatat
      541 cccgagtgga tcaagggaag acggtcagtc atcgctcgtg gcgtttgcag gaagatcgtt
      601 caaaatacga tgaagaacaa atagaagtcc tgtaggggcg acccgacaga gtttattggg
      661 caccttgctt tcacttggga gggcgccctt acaatcatat gaggagaatt tttaacatgg
      721 acgtcattaa ggaagaccaa gttttagatt gcacgggatt attatgcccg atgccaattg
      781 tgaaaacaaa gaaagcgatt gatgtattgg aatctggaca ggttttgaag atgatcgcta
      841 ctgatgcagg ctcgccgcca gatattgctg catggagccg ccagaccggg aatgaattgt
      901 tgctttcgac tgaagaaggt ggcaagttcg ttttcttttt gaagaaagcg tagggtaaag
      961 agatggcaaa atcagacccg aatgctccga aacgactggc attgatcgcc agcaaaggca
     1021 ccctagactg ggcatacccg ccttttattc tttccacggc tgcagctgcc atgggttggg
     1081 aagtgggtgt gttcttcacc ttttacggat taaccctgct caagcctgaa ctgactgccg
     1141 cggtctctcc gcttggtaac cctgccatgc cgatgaaaat gccgtttggt ccagaaaact
     1201 ttcagaatat ttcatggccc atgcccaatt tgctcatggc aaatgtgccg ggttttgaaa
     1261 gcttggcgac caatttgatg aagcagacct ttaagaataa aggtgtcgct tccattgaag
     1321 agttgcgtga tatggcggtg gaactggatg tgcgtttgat cggttgccag atgacgatgg
     1381 atgttttcgg cttcaaaaag gaagatttca tcccgcaggc tgaaatcggt ggggcggcaa
     1441 ctttccttga atatgcagcc gatgcggatg tacaattgtt tatatagagc tttttctgtc
     1501 attgcctgta ccgtgcgcag gcacgcgtgc gagggcgttt ttgctctttg cacaaagcaa
     1561 tcccgttact cataattgga ttgcttcgcc gcctaaaagc aagagcggcg gctcgcaat
//
LOCUS       DAOQNJ010000604         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_71529_length_1613_cov_8.415918, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000604 DAOQNJ010000000
VERSION     DAOQNJ010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71529_length_1613_cov_8.415918"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PLG52_13425"
     CDS             complement(<1..977)
                     /locus_tag="PLG52_13425"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNB42501.1"
                     /translation="MHKKIIMLVIVLTLLSGCTMKSPFKASPNIILILTDDMDMTLMP
                     YMPKTNQLIGEQGATFENYFITTPICCPSRATTLRGQYAHNTDILQNSPGFARFFKLK
                     EEENTLNVWLQAAGYRTALFGKYLNNYPVNAGRDYVPPGWDDWGAFIANTDDGDFFYN
                     YTMNENGTLVAYGDAPEDYSTSVIRNKSLTFIEDSVKADSPFFLFVSVYAPHGPATPA
                     PDHPGLDEALTYPQKPSFLEADISDKPQIIQALTQTGDEFDDGDANQAFQKRVASLQA
                     VDDLVADLVQTLEATGQLENTYIIFTSDNGFHLGEHQLPSGKGTPYEED"
     gene            complement(1045..>1613)
                     /locus_tag="PLG52_13430"
     CDS             complement(1045..>1613)
                     /locus_tag="PLG52_13430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB42502.1"
                     /translation="QRVVEVALKEFNRIDILFNNAGIVTKGTAETTSDETWQDTLNIN
                     VTAVWKMCQLVLPHMKKQGSGAIVNNGSDWSVVAGKNALPYIMSKGAVGMMTKAMALD
                     YARDGIRVNAVCPGDTFVDRWMEKGYFEGSDPVKIEDAIREASEYLPMGRFGKPEEIA
                     NAVVFLASDEASFVTGHLLLVDGGNTAQ"
BASE COUNT          399 a          416 c          375 g          423 t
ORIGIN      
        1 atgtcttcct catatggtgt gcccttgcca gatggcagtt gatgttcgcc aaggtgaaag
       61 ccgttatcag aggtgaagat gatgtaggta ttctccaatt gacctgtcgc ctcgagagtt
      121 tgaaccaagt ctgctaccag atcatccact gcttgcagag aggcgacacg tttttgaaat
      181 gcctgattgg catctccatc atcaaactcg tcaccggttt gggtgagggc ttgaatgatc
      241 tgcggtttgt cactgatgtc tgcttcgaga aaggatggtt tttgtggata ggtcagagct
      301 tcatccaagc ccggatggtc aggagcaggc gtcgctgggc cgtgaggcgc gtagactgaa
      361 acgaaaagga aaaagggcga gtcggcttta acgctgtctt cgataaaggt cagcgattta
      421 ttccgaatca cgcttgtgct gtaatcttca ggtgcatcac cataggcaac caatgtgccg
      481 ttctcgttca tggtgtagtt gtaaaagaaa tcgccatcgt cggtgttggc gatgaaggcg
      541 ccccaatcat cccagcccgg tgggacataa tctctgcccg cgttgaccgg ataattattt
      601 aaatattttc caaacaatgc cgtgcgatag cccgcagctt gcaaccagac gttgagcgta
      661 ttttcttcct ctttcaattt gaagaagcgc gcaaagcccg gcgagttttg caaaatatct
      721 gtattatgtg cgtattgtcc gcgcaaggtg gtggcacgtg acgggcaaca aatgggtgtg
      781 gtgatgaaat aattttcaaa ggtcgcgccc tgttcgccaa tgagttgatt agtctttggc
      841 atgtaaggca tcaaggtcat gtccatatca tcggtgagga tgaggatgat attgggcgaa
      901 gccttgaatg gacttttcat tgtgcatccg cttaacagcg taagcacaat gaccagcatg
      961 ataattttct tgtgcatgag tctccgttca aacaaggtga cagtcacttt ggaagtgact
     1021 gtcacctaaa ctttcagcag tatatcactg cgctgtattg ccgccatcga ccagcaagag
     1081 atgacccgtt acgaacgatg cttcatccga tgccaagaac acaaccgcat tggcaatctc
     1141 ttccggtttt ccaaaacgcc ccatcggcaa atattcactc gcttctcgaa tcgcatcttc
     1201 gatcttcacc gggtcagagc cttcaaaata acctttctcc atccaacgat ccacaaaggt
     1261 atcacccggg caaaccgcat tcacgcgaat cccatcgcgc gcataatcca acgccatcgc
     1321 cttggtcatc atgcccaccg cgcccttgct cataatgtat ggaagcgcat tcttgccagc
     1381 caccacagac caatccgaac cgttattcac gatcgcacca cttccctgct tcttcatgtg
     1441 cggcagcacc agctgacaca tcttccagac cgcagtcaca ttgatattca acgtatcctg
     1501 ccaggtttca tcactcgtcg tttcagcagt tcccttggtg acaatgcctg cgttattaaa
     1561 caaaatatca atgcgattaa attccttaag cgcgacttca acgactctct gac
//
LOCUS       DAOQNJ010000605         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_71691_length_1611_cov_10.460797, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000605 DAOQNJ010000000
VERSION     DAOQNJ010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71691_length_1611_cov_10.460797"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PLG52_13435"
     CDS             <1..515
                     /locus_tag="PLG52_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB42503.1"
                     /translation="GLGTQLPYRSATFDLLYCVDAIHHFGDHRAFIAEASRLLKPGGA
                     LAIIGHDPHEGPSHWYIYNYFDHVYDTDLRRYPSGNRVLQWMEQEGLKNISDQTVESI
                     HNIHVGDAVLKDPFLKHSATSQLALLNEADYQTGMERIQNALATAKEKKERIVFRSEI
                     NVKMWLGFKK"
     gene            613..1239
                     /locus_tag="PLG52_13440"
     CDS             613..1239
                     /locus_tag="PLG52_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42504.1"
                     /translation="MKTKLASWLCIFLFLTACSQSGDQPAVTQEPQQLPPTLTSTPEI
                     LPTVTPACIAQEPTQEDIDRSLAFTGQTFEQDDWEESYTVSEDRVSVSWFSNSLGAVA
                     FMEALIFPCGYEEPDLNNYFNDDNWGNVFANYESYELVSECRSDAAGLRLYQFDVESD
                     GVPYIVNYWVKNDTNTRVISFMIITPDDSFDLLDEYSYSIFPQLSNCS"
     gene            1289..>1611
                     /locus_tag="PLG52_13445"
     CDS             1289..>1611
                     /locus_tag="PLG52_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42505.1"
                     /translation="MSSRYDILFQPIKIGPVTAPNRFYQVPHCNGFGWRMPKGMAAMR
                     GMKAEGGWGVVCTEETEIHYTSDLSPYFEGRIWDDADIPTWQLMTDAVHKHGALAGIE
                     LTYNS"
BASE COUNT          421 a          441 c          361 g          388 t
ORIGIN      
        1 gcggcttggg tactcaactc ccctaccgtt ccgctacctt tgacctgctc tactgcgtag
       61 acgccatcca tcatttcgga gaccatcgcg cgttcattgc cgaagcttct cgcttgctga
      121 aaccgggcgg agcgctcgcc atcatcgggc atgacccgca tgaaggtcca tcacactggt
      181 acatctataa ctacttcgac catgtctacg ataccgacct gcgtcgttac ccctctggca
      241 atcgtgtttt gcaatggatg gaacaggaag gtcttaagaa catctcggat caaaccgtgg
      301 aaagtattca caacatccac gtgggcgatg ccgtgctgaa agaccccttc ctcaagcata
      361 gcgccacctc tcagcttgcc ttgctcaacg aagcagatta tcaaaccggc atggaacgca
      421 ttcaaaacgc tcttgctacc gcaaaagaaa agaaagagcg catcgtcttc cggtcagaga
      481 tcaatgtgaa gatgtggctc ggtttcaaaa aataatcaaa gcctgttcgc caagaacgcc
      541 cagctttcga gccgggcgtt tttctttcca catgctatac ttcgagaaaa atccaactgc
      601 acgggatatt ctatgaagac aaaactcgca tcgtggttat gtatttttct ttttctgacg
      661 gcatgttccc agtctggcga ccagcccgcc gtcacgcaag agccgcagca acttcccccc
      721 acactcacat ctacacctga aatccttcct acggtcacac cagcttgcat cgcccaagag
      781 ccaacgcaag aagatattga ccgttcgctc gcatttaccg ggcaaacctt cgaacaagac
      841 gattgggaag aaagctacac cgtatcagaa gaccgtgtat ctgtttcatg gtttagcaat
      901 tcgctgggcg ctgttgcctt catggaagcc ttgatcttcc cctgcggcta cgaagaaccc
      961 gatttgaata attatttcaa tgacgacaat tggggaaatg tatttgcaaa ctatgaaagt
     1021 tatgaactgg ttagcgagtg tcgcagtgat gctgccggct tacgcttgta tcaattcgat
     1081 gtggaaagtg atggcgttcc atacatcgtg aactactggg tcaagaacga taccaacaca
     1141 cgcgtgatca gcttcatgat cattacacca gatgattctt tcgacctgct ggatgaatat
     1201 agttactcga tctttccaca actatcaaac tgttcataac agtcgataga cgatacatac
     1261 aatcgtaaat acatcactgg aaaatctcat gtcctctcgc tacgatatcc tctttcagcc
     1321 catcaagatc ggacccgtca ccgcgccgaa ccgtttttat caggtgccgc attgcaatgg
     1381 ctttggctgg cgtatgccga aaggtatggc agccatgcgc ggcatgaaag cggaaggagg
     1441 ctggggcgtg gtctgcaccg aagagacaga gatccactac accagcgacc tctctcccta
     1501 tttcgaaggg cgcatctggg acgatgccga tatccccaca tggcaattga tgaccgatgc
     1561 tgttcacaag catggcgcat tagctggcat tgaattaacg tacaacagcc a
//
LOCUS       DAOQNJ010000606         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_71868_length_1609_cov_6.888031, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000606 DAOQNJ010000000
VERSION     DAOQNJ010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71868_length_1609_cov_6.888031"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLG52_13450"
     CDS             complement(<1..183)
                     /locus_tag="PLG52_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42506.1"
                     /translation="MRKIIVHEFITLDGVIQAPGSPTEDTDGGFTHGGWTLPYWHDEI
                     GAHFGQIFADADSLLLG"
     gene            complement(241..840)
                     /locus_tag="PLG52_13455"
     CDS             complement(241..840)
                     /locus_tag="PLG52_13455"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNB42507.1"
                     /translation="MIKLAPITLEGHGIRLEPLILEHEADLSAAAADGELWNLWFTSV
                     PKPEETKTYIETALAGYQAGHMLPWAVRELGTGKIVGSTRYHDVVANIDRVEIGYTWY
                     SKSWQKSHVNTVCKLLLLAHAFDTLGCKVVGLRTDNFNFNSQKAIEGLGAKKDGVLRH
                     HAIRRDGSVRDSVMYSILSTEWASVKQHLRFRLSRHGTL"
     gene            complement(895..1383)
                     /locus_tag="PLG52_13460"
     CDS             complement(895..1383)
                     /locus_tag="PLG52_13460"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB42508.1"
                     /translation="MDILDRLIAHDTWTTQQLLKACETLSDDLLDKEFDMDHRSLRET
                     FLHMVENLETWTDLIHERPVQEKQGKSISELRQRLVNASREFTNFARQIGRENRFDDS
                     FWDVLDNPPRLKTFGGAIGHVLTHNMHHRAQIMYLMEKVGLQDHIEGDLLSWESVAFG
                     WA"
     gene            complement(1408..>1609)
                     /locus_tag="PLG52_13465"
     CDS             complement(1408..>1609)
                     /locus_tag="PLG52_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42509.1"
                     /translation="VTFNLRDTKRIHLVFHNPMIPQVKSKILEGDYKDRRMAYFVDMK
                     EIKAKKPLLEKALRDLIKLLDK"
BASE COUNT          426 a          422 c          322 g          439 t
ORIGIN      
        1 cccgagcagc aagctatcgg catccgcaaa tatttgaccg aagtgcgcgc caatttcatc
       61 atgccagtac ggaagcgtcc aaccgccgtg agtgaagccg ccgtcggtat cttccgtggg
      121 gctgcccggt gcttgaatga caccatctaa agttatgaac tcgtgtacga taatttttct
      181 catggtttct cctatctaac attaagggcg acacattggt gaaagtttca aaaaataaaa
      241 ctataacgtt ccatgacgag ataatcggaa tctgagatgt tgctttacgc ttgcccactc
      301 agtggacaga atactataca tgacactatc gcggaccgat ccatctcggc gaatcgcatg
      361 gtgacgtaaa acgccatcct tctttgcgcc cagcccttca atggcttttt gcgaattgaa
      421 gttaaagtta tcggtgcgca gacccactac tttgcatccc aatgtatcaa aggcatgagc
      481 cagcaataac agcttacaaa ccgtattgac atggctcttc tgccagcttt tggaatacca
      541 ggtgtaaccg atctctacac gatcaatatt cgcaaccaca tcatgatagc gagtgctccc
      601 gacaattttt cccgtcccca actcgcggac agcccatggc agcatatgcc ctgcctgata
      661 tcctgccaat gcagtttcaa tataggtctt ggtctcttcc ggttttggca cagaggtgaa
      721 ccaaagattc caaagttcgc catctgctgc cgccgcggag agatcggctt catgttcgag
      781 aatcagaggt tcgagccgaa tgccatgacc ttccaatgta attggagcaa gtttgatcat
      841 ttttaccatc cataatacag agattgtttt ataaattcta cacgactcat tcgatcatgc
      901 ccatccgaat gcaacagact cccaactcag tagatcgcct tcaatatgat cctgcaaccc
      961 caccttctcc atcaaataca tgatctgggc acggtgatgc atgttatgag taagcacatg
     1021 tccgatcgcg ccgccaaatg ttttcaagcg tggcgggtta tctaaaacat cccaaaacga
     1081 atcatcaaaa cgattctcac gaccgatctg acgggcaaag ttggtaaatt cacggctggc
     1141 attcaccagt ctctgcctca attctgaaat tgacttaccc tgcttttctt gtacaggtcg
     1201 ctcatggatc aggtctgtcc aggtctccag attttcgacc atgtgaagaa atgtctcccg
     1261 aagcgagcga tgatccatat caaattcctt gtccaacaga tcatccgaaa gggtttcaca
     1321 agcctttagc aattgctgag ttgtccatgt atcatgcgcg atcaggcggt caagaatatc
     1381 cactagtcaa tttccttttg tacaatttta tttatcaagc aatttgatca gatctctcaa
     1441 cgccttttcc agcaagggtt tcttcgcctt gatctccttc atgtccacaa aatatgccat
     1501 gcgtctatct ttgtaatccc cttcaagaat cttactcttg acctgcggaa tcatcgggtt
     1561 atgaaacaca agatggattc gtttcgtatc gcgcaagtta aacgtcacc
//
LOCUS       DAOQNJ010000607         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_71870_length_1609_cov_6.218790, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000607 DAOQNJ010000000
VERSION     DAOQNJ010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71870_length_1609_cov_6.218790"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1609
                     /locus_tag="PLG52_13470"
     CDS             <1..>1609
                     /locus_tag="PLG52_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42510.1"
                     /translation="PTWQKQFSQLEVFHWMVEWATRPYYSPRGAIYEARLMEASLMNA
                     LHRAGLSFGERLHYWHGNLLFLTDIGHQSIPGNWSLAKRRYLEKCVDLYGDTLATTGI
                     HTNISLPDPLFAWDFMHLSASERGDKHLDDYKSEFYITATRLLRAFASLFIATSASTP
                     MQAQVKDGRAAVILTEFDSIRNLTFPNPREVDLPDLYRSYNDYLQISYDLVRRGVRFG
                     NNNWTPVRARSFSEPVERIISTTSEQLESLYARGLFAAGESTPPDEMAKQVERQNLMA
                     RINLPMGRVEIRVDEGGHSLDLDVANMTFRYLLMLKIYADPKFARGFRYDSEDITRAR
                     TNENLAAKHGLRAEIENPLTGKPIVVRDFLKWTLNEIKPMAQALNMWNDLSPLVEMAE
                     GGKNTAEKIRARLKMEIGNSDEVPMDLLKEFLYEHEAQVKSDVERVCADYNALGSDAS
                     KIAEYVQRGREDARQIPNAPIQYRAKTHAAVELSYPNKTAEILDLAQQLIRIPSVTAC
                     PEERLDEVHRAGSLIDDYLRNAGLDVKY"
BASE COUNT          389 a          435 c          385 g          400 t
ORIGIN      
        1 ccctacgtgg cagaagcaat tcagccaact cgaagtcttt cactggatgg tggagtgggc
       61 gacccgcccg tattattctc cacgtggggc gatctatgaa gcccgtctga tggaagcctc
      121 attgatgaat gctttgcatc gcgccgggct gagctttggc gaacggctgc attattggca
      181 tggtaatttg ctcttcctta ccgatattgg tcaccaatcg attcccggca attggtcttt
      241 agccaagcgc cgctatctcg aaaaatgcgt tgacctctat ggcgacaccc tcgccaccac
      301 aggcattcac accaatatct cgttgccaga cccactcttc gcatgggatt tcatgcatct
      361 ctctgccagc gaacgcggcg ataaacattt ggatgattac aagtctgagt tttatattac
      421 cgccacacgc ctcttacgtg cttttgcttc tctcttcatt gcgacgagtg cttctacgcc
      481 gatgcaggct caggtcaaag acggacgcgc cgcagtcatc ctcaccgagt tcgactcgat
      541 ccgtaatctc accttcccga atccgcgcga agtggacttg cccgacctct accgttctta
      601 taacgattac ttgcaaatct cctatgacct tgtccgccgt ggcgtgcgct tcggtaacaa
      661 caactggaca cccgtccgtg cccgcagttt ctctgagccg gtagagcgca ttatttccac
      721 gacgagcgaa caacttgagt ctctctatgc tcgcggactc ttcgccgcag gcgaatccac
      781 gccgccagat gaaatggcaa aacaggttga acgtcagaac ctgatggctc gtatcaacct
      841 cccaatggga cgtgtcgaaa tccgcgtaga cgaaggcggt catagccttg accttgatgt
      901 tgccaacatg actttccgtt atttgctcat gttaaagatc tatgcagacc cgaaatttgc
      961 tcgtggcttc cgttacgaca gtgaagatat tactcgtgcg cgcaccaatg aaaatttggc
     1021 agccaaacat ggcttgcgcg ctgaaattga aaatccactc acgggcaaac ctatcgttgt
     1081 acgagatttt ctcaagtgga cgttgaacga gatcaaaccc atggcacaag ccttgaatat
     1141 gtggaatgac ttgagtccgc ttgtggaaat ggctgaaggc ggcaaaaata ctgcagaaaa
     1201 gattcgtgcc cgtctcaaga tggaaattgg aaatagcgac gaagtcccca tggacttgct
     1261 caaggaattt ttgtatgagc atgaggcaca agttaaatcc gatgtcgagc gtgtctgcgc
     1321 agattacaac gccttgggta gtgatgcgtc caaaattgct gaatatgtcc aacgtgggcg
     1381 cgaagatgct cgtcaaatcc cgaatgcacc cattcaatac cgcgcaaaga cacacgctgc
     1441 ggttgaactt tcctatccga acaagactgc cgaaattctt gatcttgccc agcaattgat
     1501 tcgcattcca tcggtcactg cctgccctga ggaacgtctg gatgaagtcc accgcgccgg
     1561 gtctttgatc gacgattatt tacgcaatgc gggtttggat gtgaaatac
//
LOCUS       DAOQNJ010000608         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_71943_length_1608_cov_9.026401, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000608 DAOQNJ010000000
VERSION     DAOQNJ010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71943_length_1608_cov_9.026401"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1475
                     /locus_tag="PLG52_13475"
     CDS             84..1475
                     /locus_tag="PLG52_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNB42511.1"
                     /translation="MNKNLENDQNLIPSLAEHTKDIAQNYLNEIDQRPPATAYAQKQN
                     LDLSLTGIGAEKTLDLFMERYGNHMPASNGPRFWGLVTGGTTPASLLGDWLTSVYDLN
                     LSHAGNSPAPDIETEAISMLRELFGLPASFSGTFVTGATMSNFAGLAIAREWVGKHYG
                     KSVSQEGLHAIPPVKILSCEAHASSFKALGMLGLGRNSLKVIPKLPENREAVNVVELR
                     KALEGMNGEPCIIIANAGTVNTVDFDDIQAIVALKSEFKFWLHVDAAFGGFAACSPKF
                     KHLLNGIEYADSITIDAHKWLNVPYDSAMIFTQHRDLQIAVFQNSGAYLGAIAEPVDF
                     IHLSPENSRRMRALPAWFSLMAYGKEGYQDIVERNCAMAERLGEKISTSKEFRLLAPV
                     RMNVVCFTLSGDVDAEKVKAFLSKLRDDGRVFMTPTTYLGTPGIRAAFSNWRTEEKDI
                     EIAWRAMLDCISQ"
BASE COUNT          442 a          377 c          388 g          401 t
ORIGIN      
        1 aatcaagatt tttcgatcac agtattgagg aatatccaat cgagtaaaat cttacgtgaa
       61 aatcaaacct gaaagaagcc cttatgaaca aaaaccttga aaatgaccaa aaccttatac
      121 cttctttagc agaacataca aaagatatag cacaaaacta tcttaacgaa attgaccaac
      181 gcccgcctgc aacagcgtat gctcaaaaac aaaaccttga tctctccctt acgggaatcg
      241 gcgcagaaaa aacattggac cttttcatgg agcgctacgg aaatcacatg cccgccagca
      301 atggaccgcg attctggggt ttggtcactg gcggcacgac tcccgcttcc ctgctcgggg
      361 attggctgac ttctgtttac gatctgaatt tgagccacgc tgggaattct cccgcgccgg
      421 atattgaaac ggaagcgatt tccatgctgc gcgagttatt cggtttgccc gcctctttct
      481 cagggacgtt tgttaccggc gcgacgatgt ctaacttcgc cggtcttgcc attgctcgcg
      541 aatgggttgg gaagcattat ggaaaatcgg tttcgcaaga agggttgcat gccattccac
      601 ccgtcaaaat attaagttgt gaagctcatg ccagctcatt caaggcattg ggaatgcttg
      661 ggctgggaag aaactccctg aaagtcatcc ccaagcttcc cgaaaataga gaagcggtga
      721 acgtcgtcga attgcgaaaa gctttggaag ggatgaatgg cgaaccctgc atcattatcg
      781 ccaatgcagg gacggtcaac accgtggact tcgatgacat ccaagccatt gtggcgttga
      841 aatctgaatt caagttctgg ctgcatgtgg atgcggcatt tggcgggttt gccgcgtgtt
      901 caccaaaatt caagcatttg ctcaacggga ttgaatatgc agacagtatc acaatcgatg
      961 cgcataaatg gctgaatgtg ccttacgact cggcaatgat ctttacgcaa catcgcgact
     1021 tgcaaattgc agtttttcaa aacagtggcg cttatcttgg tgcaattgcc gaaccggttg
     1081 attttatcca tctctcgcca gaaaactcgc gccgtatgcg tgcactgcct gcctggttta
     1141 gtttgatggc atatggcaaa gaaggttatc aagacattgt ggaaagaaat tgcgcgatgg
     1201 cagagagact cggcgagaag atttcaacca gcaaggaatt ccgtctgctc gcgccagtgc
     1261 ggatgaacgt agtctgtttt acgctgagcg gtgatgtgga tgcggaaaag gtcaaggctt
     1321 tcttgtctaa actacgggac gatggacgcg ttttcatgac acccactact tatctgggca
     1381 cacccggaat ccgcgcggca tttagcaatt ggcgtaccga agaaaaagat atcgagatcg
     1441 cctggcgagc aatgctagat tgtataagcc aatagaatca aaaaaactcc gaagcgaatg
     1501 cttcggagtt ttttgaattt actttatggc acaggcaaag cttcgcacag cgcacgataa
     1561 tcttcttttt caaagaacaa tgtccactcc gctgcgctaa agttcttt
//
LOCUS       DAOQNJ010000609         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_72933_length_1596_cov_9.012979, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000609 DAOQNJ010000000
VERSION     DAOQNJ010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72933_length_1596_cov_9.012979"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="PLG52_13480"
     CDS             complement(<1..1237)
                     /locus_tag="PLG52_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB42512.1"
                     /translation="MNNIAASFKKLLQYPSALAGTFVILVMVAVSIYAMVTIPYSEAV
                     RLWRGGEEVWYQNPKFAPPAWFNLFSSKKYAESFAVSTANGEMEKTVVPGNDGVNTYT
                     MTYEFDFKYDVYPQDMIVYVNALFDQKQPFISMEWITPDGRTIRVSNFATAAKSTYRF
                     SQDDKLAAKLRAEDPIPALFSDPDTGEVLKGPYKLVITGTTFEPNSNIDVELVVHGQV
                     YGLAGTDHARRDLTLPLLWGAPVALAFGLIAAIGTSVLTMVIAAVGTWYGGWVDQLVQ
                     RITEVNLVLPFFAIMIMIGTFYSRSIWTIISATIVLNIFTGAILGYRAIFLQVKESMY
                     IEAAKAYGATNWRIIFHYLIPRMIPLLIPGLVQAVPAFVFLEASLAVIGLGDPVLPTW
                     GKIIQDAQANGALYKGYYYW"
     gene            complement(1241..>1596)
                     /locus_tag="PLG52_13485"
     CDS             complement(1241..>1596)
                     /locus_tag="PLG52_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB42513.1"
                     /translation="NYRTFFLIYSSEDYVDMARAKGLHAKDIERRYILRPTLPNIITN
                     FSLLIIALWTGALFTETVFLWPGLGRTLYQAVGLYDTPIIVGSTIIYAYLLAMTVFLL
                     DFIYAAVDPRVRFGN"
BASE COUNT          495 a          364 c          365 g          372 t
ORIGIN      
        1 cccagtagta atatcctttg tataaagctc catttgcttg agcatcttga ataattttcc
       61 cccaagtcgg taggacgggg tctcctaaac cgatgacggc aagagacgcc tcaaggaaca
      121 caaaggctgg cacagcttga accaaacccg gaatcaacaa ggggatcatg cgtggaatga
      181 ggtaatgaaa aataatacgc caatttgttg cgccgtatgc ttttgccgct tcaatgtaca
      241 ttgactcttt gacctgaaga aatatggcac gatacccaag aatggcaccc gtaaagatat
      301 tcaagacaat cgttgcactg ataatggtcc aaatactacg ggaataaaat gtaccgatca
      361 tgatcatgat cgcaaaaaac ggcaacacca aattgacttc ggtaattcgt tgcaccaact
      421 ggtcgaccca gccgccatac catgtgccaa cagcagcaat gaccattgtt aagaccgaag
      481 tgccgatagc tgcaataaga ccaaacgcca atgccacagg agcaccccac aaaagcggta
      541 gggtcaaatc acggcgggca tgatccgtcc ctgccaagcc atagacctgt ccgtgaacca
      601 cgagttctac atctatgttc gagttaggct caaaagtggt gccagtaata acgagcttat
      661 agggtccctt taatacctca ccggtatctg ggtcagagaa caaggctggg atggggtctt
      721 ctgcccggag tttggctgcc agtttatcgt cttgtgagaa gcgataggta gacttggctg
      781 cggtagcaaa atttgaaaca cgaatcgttc gcccatcagg tgtgatccac tccatcgaaa
      841 tgaacggctg tttctggtca aaaagggcat tgacataaac gatcatatct tgagggtaga
      901 catcgtactt aaaatcgaac tcgtatgtca ttgtgtatgt attaacgcca tcgtttccag
      961 ggacaaccgt cttttccatt tcgccatttg cagtgcttac cgcaaaagac tcagcatatt
     1021 ttttgctgga aaataaattg aaccaagcag ggggagcaaa ctttgggttt tgataccaca
     1081 cttcttctcc accgcgccat aaacgcacag cttcagagta tggaatggta accatggcat
     1141 aaatggaaac cgccaccata accaaaatga caaaggtgcc tgccaaggca gaaggatatt
     1201 gaagaagttt tttgaaggaa gctgcgatat tgttcatggc ttaatttcca aaacgcacac
     1261 gtgggtcaac tgcggcataa ataaaatcaa gcaggaaaac cgtcatggcg agtaaatacg
     1321 catagatgat agtagagcct acaatgatcg gtgtatcgta taaaccaacc gcttgataca
     1381 gagttcgccc aagcccgggc caaaggaaga cagtttcagt aaacaaagca cctgtccaaa
     1441 gcgcaatgat gagtagcgaa aagttcgtaa tgatattagg taaggttggg cgcaaaatat
     1501 accggcgctc aatatctttg gcatgcaagc ctttggcgcg cgccatatca acataatctt
     1561 cgctggaata aatcaaaaag aaagttcgat agttgt
//
LOCUS       DAOQNJ010000610         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_73190_length_1593_cov_5.719116, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000610 DAOQNJ010000000
VERSION     DAOQNJ010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73190_length_1593_cov_5.719116"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PLG52_13490"
     CDS             <1..1014
                     /locus_tag="PLG52_13490"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB42514.1"
                     /translation="KCTMETVELNRAFWLDRPTFVTGGTGLVGSWLVKRLVAAGADVV
                     CLVRDWVPQSELARTGLIEKVKVVRGDIRDREILERTLGEYEIDTVIHLAAQTIVTIA
                     NRNPVSTFETNIAGTWNLLEAARRSPKVKQLVMASSDKAYGDQEILPYDEHTPLQGQH
                     PYDVSKSAADLIASTYAKSYDLPVAITRCGNFYGGGDLNWNRIIPGTIRSILRGQRPI
                     IRSDGQYIRDYFYVEDGAAAYMLLAEQLAANPNLKGESFNFSNEIQVAVLEIVSKILR
                     LMNSDLKPQVLNEASNEIRHQYLSAEKARRLLNWHPLFNLDQALSSTIDWYKEFFTHE
                     RTI"
     gene            998..>1593
                     /locus_tag="PLG52_13495"
     CDS             998..>1593
                     /locus_tag="PLG52_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB42515.1"
                     /translation="MSERSDRLRAKILELTAEYVAEEFPEKEFIPGESSVAVAGRVFD
                     AADVQSLVDSSLDFWLTAGRFSRQFERDFAKFFNLRHSILVNSGSSANLLALTCLTSP
                     TLKERQLKPGDEVITVATGFPTTVNPIIQNNLVPVFLDVDIPTYNIDVSQLDAALSSK
                     TRAVMVAHTLGNPYKLEAVTAFCKKHNLWLVEDCCDAVG"
BASE COUNT          391 a          396 c          381 g          425 t
ORIGIN      
        1 aaatgcacca tggaaactgt ggagttgaat cgcgcgttct ggctcgaccg cccaactttt
       61 gttacaggtg ggaccggact agttggttcc tggcttgtca aacgtctcgt cgcagcaggt
      121 gcggatgtag tttgtctcgt gcgcgattgg gtgccacaaa gcgaactggc gcggacgggg
      181 ttgatcgaaa aagtaaaagt tgtgcgcggc gacattcgtg accgtgaaat tttggaacgt
      241 acattgggtg aatatgagat cgatacggtc attcatcttg ccgcccagac cattgtgact
      301 attgcaaacc gcaaccctgt ttctactttt gaaaccaata ttgccggaac atggaattta
      361 cttgaagctg cacgtcgcag ccccaaagta aaacaattgg tgatggcatc ttcagataaa
      421 gcctatggcg accaggaaat tttgccgtat gacgaacata caccgcttca aggacagcat
      481 ccatatgatg tcagcaaatc tgcggcggat ctgatcgcct cgacctatgc caagagctac
      541 gatctgccgg tagcgattac acgctgcgga aatttctatg gcggcggcga cttgaattgg
      601 aaccgcatta tccctggtac catccgctct atcctgcgcg gacaacgccc tattatccgc
      661 tcagatggac aatacatccg tgattatttt tatgtggaag atggcgcagc tgcttatatg
      721 cttttggctg aacagcttgc tgcaaaccca aacctcaaag gcgagtcgtt taacttttct
      781 aatgaaattc aagttgcggt attggagatc gtctcaaaaa ttttacgtct aatgaactca
      841 gacctcaaac cgcaggtctt gaatgaggcc tcgaatgaga tccgccatca atacctgagc
      901 gcagagaaag cccgccgcct cttaaactgg catccacttt tcaatttgga tcaagcctta
      961 tcatctacca ttgattggta caaggaattt tttacacatg agcgaacgat ctgatcgact
     1021 gcgggcgaag atcctcgaat tgaccgctga atatgtcgcc gaggaatttc ctgaaaagga
     1081 gttcatccct ggcgaatcat ccgtggcggt ggcggggcgc gttttcgatg cggcagacgt
     1141 gcaatcactg gtcgactcca gcctcgattt ttggctgact gccgggcgtt tctcacgcca
     1201 atttgagcgc gactttgcga aattctttaa tctgcgccac tccattttgg tgaactcggg
     1261 ttcttctgcc aatttacttg cgttgacttg tctcacctcg ccgacgctta aagaacgcca
     1321 gctcaaaccg ggtgatgaag tcatcaccgt tgccacgggc tttcctacca cggtcaaccc
     1381 aatcattcag aacaatcttg tcccagtttt cttggatgtg gatattccca catacaacat
     1441 tgacgtttct caattggatg cagctttgtc cagcaagact cgtgccgtca tggttgcaca
     1501 tactcttggt aacccatata aacttgaagc agtcactgcg ttttgtaaaa aacataatct
     1561 ctggcttgtt gaagattgct gtgacgctgt tgg
//
LOCUS       DAOQNJ010000611         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_73265_length_1592_cov_10.517892, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000611 DAOQNJ010000000
VERSION     DAOQNJ010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73265_length_1592_cov_10.517892"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1160
                     /locus_tag="PLG52_13500"
     CDS             219..1160
                     /locus_tag="PLG52_13500"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNB42516.1"
                     /translation="MKLTTNNIIIIFGSLILCMLALIPITVLAMPLLETNQPDTQATM
                     QAMVTQTVIALTLNAPTQTPLPPTATPVPATNTPVPTVTTLPTVSYCDWMAFVTDISV
                     PDGTNFSAGETFVKTWRIKNRGTCTWTPDYTLVFNGGDQLGSTTSVRLPGYVSPGQYI
                     DVSITFTAPNNPGSYRGYWIMRNTSGALFGTGDKANAPIYVDIRVRQSDPLPHGTISG
                     NLCYPSEFNPRMTLYIENANTGERIQFAITENQYVYEVLVPAGRYYAYAWAPSYSLEG
                     AYAHDNGLMKSFFVESGKETPFINLCNWGPYGHAKGE"
     gene            complement(1212..>1592)
                     /locus_tag="PLG52_13505"
     CDS             complement(1212..>1592)
                     /locus_tag="PLG52_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42517.1"
                     /translation="HKDFSGVEKFIRSNKRGHLIVPAGSARDMFMYIKNDMVDEAQDF
                     FAKRLEGKADVVKTETLISEGYFGTEISQRFLERVGNVVILPYRYESVWWYEKDKFEQ
                     RFRGHHGGLTPQEMETVLYSMEIG"
BASE COUNT          417 a          394 c          364 g          417 t
ORIGIN      
        1 aggaaggtat cgataaattc atccaaagaa tatttttgag aggcttccag ccaagtgatt
       61 tgcttggtaa atgcggaaag aataagaggg tttaattcat cggaggagat cataggttgg
      121 attttatctt atttttttta gtactttggt actggaaatg atgggaacaa tttgtgccgg
      181 gcaaacgttc taggtgggca aatgaaaaag aggactttat gaaacttacg accaataaca
      241 tcattattat tttcggtagc ctgatcttat gtatgctggc gctgattccg attaccgtgc
      301 ttgccatgcc tttattagag acaaaccaac cagacacgca agccacgatg caggcgatgg
      361 tgacccaaac cgtgattgcc ttaacgctta acgccccgac acaaaccccg cttcctccta
      421 cggcgacgcc tgttcctgcc acgaatacac ccgtgcctac ggtgacaacc cttcctaccg
      481 tcagctactg tgactggatg gcattcgtca cagacatctc tgtacccgac gggacgaatt
      541 tttctgccgg cgagaccttt gtcaaaacat ggcgcatcaa gaatcgcggc acatgcactt
      601 ggacgccaga ttacaccctt gtgttcaacg gcggtgacca gttaggttcc acaacttcgg
      661 tgcgtttgcc tgggtatgta tctccggggc aatacattga tgtgtccatt acattcacag
      721 cgccaaataa ccccggctcg tatcgcggat attggatcat gcgcaacaca tctggcgcgc
      781 tcttcggcac cggtgataaa gccaatgccc ccatctatgt ggacattcgt gtgcgtcaga
      841 gtgacccgct gcctcatggc actattagtg gcaacctatg ttacccgagt gagtttaatc
      901 cgcgcatgac gctttacatc gagaatgcta atacaggcga gcgcattcag ttcgcgatta
      961 ctgagaacca gtatgtgtac gaagtgctag tgcctgccgg acgctactac gcttatgctt
     1021 gggctccgag ctatagcttg gaaggagcat atgctcacga taatgggctg atgaaatcct
     1081 tctttgtgga aagcggcaag gaaacaccgt ttatcaacct gtgtaactgg ggaccatacg
     1141 ggcacgcgaa aggcgaataa aaaatcaaaa aagcgaccta ttacaggtcg cttttttgat
     1201 tgagtatatt gttatccaat ttccatggaa tacaagaccg tttccatctc ttgcggagtt
     1261 aacccgccgt ggtgcccacg aaagcgctgc tcaaatttat ctttctcata ccaccatact
     1321 gattcatagc gatacggcaa aatgaccaca ttcccaactc gctctaaaaa tcgctgagag
     1381 atctctgtcc caaagtaccc ttcagagatg agggtttcgg tcttgaccac atcggctttg
     1441 ccttccaaac gctttgcaaa aaagtcttga gcttcatcta ccatatcgtt cttgatatac
     1501 atgaacatat cgcgtgcaga ccctgccggg acgatcaaat gtccgcgttt gtttgagcgg
     1561 ataaactttt cgacaccaga gaaatctttg tg
//
LOCUS       DAOQNJ010000612         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_73360_length_1591_cov_12.937500, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000612 DAOQNJ010000000
VERSION     DAOQNJ010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73360_length_1591_cov_12.937500"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PLG52_13510"
     CDS             complement(<1..1382)
                     /locus_tag="PLG52_13510"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNB42518.1"
                     /translation="MKSLRWKQLALVALIIVGCTGPGSGSGVGSIFATDTPLPTPSVG
                     ITPAPNVDSAVTAFFDALKGDNYEEMYAMLSQSSRDALTLEDFSKRWTNALNEMSASS
                     FDVTVLSKSITPFNAEVKYSITYKTALVGDIQREMLMRLTNEDSNWKVQWDDALILPE
                     LAGGNLLKMEYSIPARGDIYDSKGLPIATQSDAFAFGIQTDQIDNDMIGALTTELGRL
                     CGLDPLDIQAQIDAAGPGWYLPMCEGTREEAQRLLSINPGGLVIQEYNSRYYFRGGLA
                     PQAVGYTLAISPEQLNQYKRLGYSGSERIGYTGVEQWAEDYLAGKHGGILRVVSPTGQ
                     VIATLGQSQPAPADSVYLTLDSNMQYYAQAAVEYFRGAIVVMEVDTGRIIAMASGPDF
                     DPNVFDSNNRNNQAVGNIFNDTRNPLLNRAAQGQLPLGSVFKIITFSAALESGLFLKD
                     TTYNCQYDFT"
     gene            complement(1401..>1591)
                     /locus_tag="PLG52_13515"
     CDS             complement(1401..>1591)
                     /locus_tag="PLG52_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42519.1"
                     /translation="FKPVSSLEGCRAGVMGLPMCHVVRTLKHFNISSFADVPSACQSL
                     LQYDCKVSPLILNGEIVN"
BASE COUNT          448 a          391 c          347 g          405 t
ORIGIN      
        1 tctgtaaaat catactgaca attgtatgtt gtatctttta agaacaatcc gctttcaaga
       61 gctgccgaga aggtaatgat cttgaaaaca gaccctaatg ggagttgacc ctgagcagca
      121 cggttcagaa gcgggtttct cgtgtcatta aaaatattcc ctacagcttg gttattgcgg
      181 ttattgctat caaaaacatt cgggtcaaaa tcaggtcccg atgccatagc gatgatacgc
      241 ccggtatcga cttccatcac tacaatggca ccacggaaat attccactgc cgcctgagcg
      301 tagtattgca tattgctatc gagagtgaga tataccgaat cagcgggcgc aggttgactt
      361 tgccccaagg tggctatcac ctgccccgtt gggctaacaa cacgtaaaat gccgccatgt
      421 ttgccggcta aataatcttc tgcccattgc tcaacgccgg tataaccaat tcgctcacta
      481 ccactatagc ctaaacgctt gtattgatta agttgttctg gagaaatcgc cagggtgtat
      541 cccacggctt gcggagccaa cccaccgcgg aagtaatagc gtgagttata ttcttgaatg
      601 accaaaccac cagggttaat agagagtaaa cgctgagctt cttcacgcgt tccttcacac
      661 atcggcagat accaaccggg tccggcagca tcgatctgcg cctgtatatc aagcggatcc
      721 aagccacata aacgcccaag ttcggttgtc aatgcgccga tcatgtcatt gtcaatttga
      781 tcggtttgaa tcccaaaagc aaacgcatca gattgagtgg caattggcaa gcctttgcta
      841 tcgtagatat ctccacgggc agggatgcta tattccatct ttagtaagtt gccaccagcc
      901 aattcaggaa ggatcagggc gtcgtcccat tgcactttcc aattgctatc ttcattcgta
      961 aggcgcatca acatttcacg ttgaatgtcg ccaacaaggg cagtcttata cgtaatactg
     1021 tatttaactt ctgcattaaa aggcgtaatg gattttgaga gaacggtcac atcaaaactg
     1081 gatgcgctca tttcatttag agcattcgtc caacgctttg agaaatcttc caaggtcaac
     1141 gcatcgcggc tggactgtga caacatggca tacatttctt cgtaattgtc accttttaac
     1201 gcatcaaaaa acgcagtgac tgctgaatcc acgttaggtg cgggagtgat tccgaccgac
     1261 ggggttggca agggtgtatc ggttgcaaaa atcgaaccga ctccactccc tgagccaggt
     1321 ccggtgcagc cgacaatgat taaagcaacg agagctaatt gtttccagcg taatgacttc
     1381 attacgtttc ctttcaattt ctaattgacg atctcaccgt tcaaaatcaa cggcgagacc
     1441 ttacaatcat actgtaaaag agattggcat gcagatggaa catctgcaaa agatgaaata
     1501 ttaaagtgtt tcaaagtgcg caccacatga cacataggca aacccatcac cccggcacgg
     1561 caaccttcca atgaagagac cggcttaaac t
//
LOCUS       DAOQNJ010000613         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_73704_length_1587_cov_8.406658, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000613 DAOQNJ010000000
VERSION     DAOQNJ010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73704_length_1587_cov_8.406658"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..1104)
                     /locus_tag="PLG52_13520"
     CDS             complement(241..1104)
                     /locus_tag="PLG52_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42520.1"
                     /translation="MNKRTLKNDFLEIEYLTSSLRISGLTPTGKTNLLADMSHQPPIP
                     TPYGDFYFRGGHRLWHAPEAMPRTYAPDTGELKITDLPNGVILETQTEPGTGIRKRIE
                     IQLASDKPSISLTHTLINDGLWSVELAPWTITQLRLGGVAILPMPVGNVDEAGLLPNR
                     NLSFWPYSRINDPRLSLRDDYVIFKADALPAFKLGYFNPHGWLAYYIDGILFRKTFSA
                     QDDLVYPDNNCNAEMYCNHQFVELESLGPLTRLRPGTSISHFENWELVEGLENLSIEI
                     QRVISGSDFFS"
     gene            complement(1112..>1587)
                     /locus_tag="PLG52_13525"
     CDS             complement(1112..>1587)
                     /locus_tag="PLG52_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB42521.1"
                     /translation="VQIIFNIFRQRPAELFFPEALRRKVGILARVPLASGLLTGKLSA
                     KSTFEKDDHRAFNRHGESFDRGETFSGVDYETGLQAVEELRPFIPAGWTMPQFALRWI
                     LIHDAVTCTIPGAKNPAQAEDNVRAADLPSLSAEAMEKIQVVYESRIRSLVHNYW"
BASE COUNT          415 a          365 c          413 g          394 t
ORIGIN      
        1 ggatgttccc ccattatgac atataatgcc tccggtcaca aactgtgctt gcctcctttc
       61 aaaaaacata cctcaacaga ttgatcaaaa catgattatc tccaacgtag cacagatcaa
      121 agcatcagtg tgatgttggc tgcactaaaa ggctgtggat ggggtttcga tcacagcgtt
      181 tttcgagtct gctaaccaaa agaacctcag aaagtgaagg ggaattcatg catgtggttt
      241 tcaggaaaaa aagtcactgc ctgaaattac ccgttggatt tcaattgaaa gattttcgag
      301 cccttcaacc aattcccagt tttcaaaatg gctaatcgat gttcctgggc gtaatcttgt
      361 taatggtcct aggctttcaa gttctacaaa ctgatgattg cagtacattt cggcattgca
      421 attgttatca gggtaaacga gatcatcttg agcggagaat gtcttgcgaa acagaatacc
      481 gtcgatatag tatgccagcc aaccatgtgg attgaaatat cccagcttga atgcgggcag
      541 ggcatcagct ttgaaaatga catagtcatc acgcaggctc aggcgcgggt cattgatgcg
      601 cgagtagggc cagaacgata gattgcggtt gggtaaaagc ccagcttcat ccacattccc
      661 tacgggcata ggcaaaatgg caaccccacc caggcgtagt tgagtgatcg tccatggtgc
      721 aagctcaaca gaccataaac catcattgat aagagtgtga gtgagcgaga tagagggttt
      781 gtccgatgca agttgaattt cgatgcgctt gcgaatgccc gtgccgggtt ctgtttgcgt
      841 ttcaaggatc acgccatttg gcaggtcggt gatcttcaac tcgccagtat caggtgcata
      901 ggtacgaggc atggcttcgg gtgcatgcca cagacgatga ccgccacgaa aataaaaatc
      961 gccatacggt gtggggatgg gcggctgatg actcatatct gccaacagat tcgttttgcc
     1021 tgttggcgtc agtcccgaga tgcgcaacga gctggtgaga tattcaattt cgagaaaatc
     1081 gtttttgaga gtgcgtttgt tcatgatttg atcaccaata attgtgaaca aggctgcgga
     1141 tgcgcgactc gtatacgact tgaatttttt ccatcgcctc agcactcagg gatggcaggt
     1201 ctgccgcccg aacattatcc tccgcctgag ccggattctt cgcgccgggg atggtgcatg
     1261 tgacagcgtc gtgtatcaga atccagcgca gggcaaattg cggcattgtc caaccagcag
     1321 gaatgaacgg acgtaattct tcaacggctt gcaaaccggt ttcgtaatct acgccagaaa
     1381 aggtttcgcc gcgatcaaac gattcgccgt gacgattgaa ggcgcgatgg tcatcttttt
     1441 caaaggtcga tttagcactg agcttgccgg tcaacagtcc actggcgagc ggaacgcgtg
     1501 ccagaatccc aaccttgcgg cgaagcgctt ctgggaagaa gagttcagcc ggacgttggc
     1561 ggaagatgtt gaaaatgatc tgcaccg
//
LOCUS       DAOQNJ010000614         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_73783_length_1586_cov_9.177662, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000614 DAOQNJ010000000
VERSION     DAOQNJ010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73783_length_1586_cov_9.177662"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PLG52_13530"
     CDS             <1..1092
                     /locus_tag="PLG52_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB42522.1"
                     /translation="PYEILCGVLMKRGILLLIGILLLFSALIAGYILTQTYNFNGYVQ
                     PEPIKAPETMLRSANGPVRLSDFQGKIVLLYFGYTFCPDVCPTSLANIKLALADLTPE
                     EAAQVQVIFVSVDPARDTPEALGQYVNMFQDNFIGATGTRDEIDWMVNAFGAKYTIES
                     PDVNGNYNVSHSAFTMVIDRNGFYVLNWAHGTPSTDMSADLHHIINKGIPVSAQILAG
                     PTYTPVVCAVTLIPSHVQNGQWLYEHHCAQCHGMDLAGNPQWQVELADGSHLAPPLNG
                     SGSAWKYSRTDLLAIIKEGRNLEKPIHMPAFKSNLADWEIDFILTYIQSTWDINQLNY
                     QHGFMTLTPNPTSTPAAPLAPLVTVTPSP"
     gene            1173..1445
                     /locus_tag="PLG52_13535"
     CDS             1173..1445
                     /locus_tag="PLG52_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42523.1"
                     /translation="MKNGTCPKCGSNEILSELPLHGGEGHPSYVDIVQPEPPNRPFIW
                     VAQSEQSKFVAYICGACGYSEFYAVRHQALNEGRKKGFKPRFDYTA"
BASE COUNT          386 a          459 c          352 g          389 t
ORIGIN      
        1 ccctacgaaa tcttgtgcgg agttcttatg aaacgtggca tccttctctt aatcggcatc
       61 ttgttattat tctcagccct tatcgctggc tatatcctca cccaaaccta caacttcaac
      121 ggctatgttc agcccgaacc gatcaaagca cctgagacca tgttacgctc cgcaaatgga
      181 ccggtccgct tgagcgattt tcagggcaag attgtgttac tgtatttcgg ctacaccttc
      241 tgcccagatg tttgccccac ctcgttggcg aacatcaagc tggcgctcgc agatttgacc
      301 ccggaagaag cggctcaggt gcaggtcata ttcgtttctg tagacccagc tcgcgacacg
      361 cccgaagcgc tcggtcagta tgtcaacatg tttcaggata acttcatcgg cgccaccggc
      421 acgcgtgacg agatcgattg gatggtgaat gccttcggcg ccaagtacac catcgagtcg
      481 cccgatgtca atgggaacta caacgtcagt cactcggcgt tcacaatggt gatcgaccgc
      541 aacggctttt acgtgttgaa ttgggcgcat ggcacaccgt ctacagatat gagcgcagac
      601 ctgcatcaca tcatcaacaa aggcattccg gtcagtgcgc aaattttggc gggtccaacc
      661 tatacgccag tcgtttgtgc ggtcacgttg attccgtcgc acgtccaaaa cggtcaatgg
      721 ctgtatgaac atcattgcgc acaatgtcac ggcatggact tggctggcaa cccacagtgg
      781 caggtagagt tggcggatgg ctcgcatctg gctcccccgc tcaacggcag tggaagcgcg
      841 tggaagtatt ctcgaaccga cctgcttgcc atcatcaaag aaggacgcaa cctcgagaaa
      901 cctattcaca tgcctgcctt caaatcaaac ctcgccgatt gggagatcga cttcatcctc
      961 acctatattc aaagcacttg ggatattaac caactgaact atcaacacgg attcatgacc
     1021 ctcactccca acccaaccag cacccctgcc gcgcctcttg ccccgctcgt aacggtgacg
     1081 ccctctccat aattttctcc accctttggc ggatactcct ttacaaaccc acaggctatg
     1141 atgagtgcat ctcatacttt aggagcttca tcatgaaaaa cggaacttgc cccaaatgcg
     1201 gctcgaacga aatcttaagt gaacttccct tacatggcgg cgaagggcat ccatcatacg
     1261 tggatatcgt ccaaccagag ccgccaaacc gtcccttcat ttgggtggcg caaagcgaac
     1321 aaagtaaatt tgtggcatat atttgtggtg cttgcggcta ttctgaattt tatgccgttc
     1381 gccatcaagc tctaaacgaa ggtcgaaaaa aaggcttcaa accgcgcttc gattacactg
     1441 cctgacagac tctcaatagt ctttgacagc cctaccctgc tatcctgacc tcaacacaag
     1501 gaggcacggg tatgaaagtc atctctattt ttctggcgct aatcaacttc cttacagggg
     1561 ttttgttaat tctctcctgc atctct
//
LOCUS       DAOQNJ010000615         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_73880_length_1585_cov_7.198693, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000615 DAOQNJ010000000
VERSION     DAOQNJ010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73880_length_1585_cov_7.198693"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..1102)
                     /locus_tag="PLG52_13540"
     CDS             complement(239..1102)
                     /locus_tag="PLG52_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42524.1"
                     /translation="MNKRILKNDFLEIEYLTHSLRISGLTPTGKKNLLADMSHQPPIP
                     TPYGDFYFRGGHRLWHAPEAMPRTYAPDTGELKITDLPNGVILETQTEPGTGIRKRIE
                     IQLAPDKPSATLTHTLINDGLWTVELAPWAITQFNLGGSVILPMPVGNVDEAGLLPNR
                     NLSFWPYSRINDPRLSLRDDYVIFKADALPAFKLGYFNPHGWLAYYIDGILFRKTFSA
                     QDDLVYPDNNCNAEMYCNHQFVELESLGPLTRLRPGTSISHFENWELVEGLENLSSEI
                     QRAISGSDFFS"
     gene            complement(1110..>1585)
                     /locus_tag="PLG52_13545"
     CDS             complement(1110..>1585)
                     /locus_tag="PLG52_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB42525.1"
                     /translation="VQIIFNIFRQRPAELFFPEAIRRKVGILARVPLASGLLTGKLSA
                     KSTFEKDDHRAFNRHGESFDRGETFSGVDYETGLQAVEELRKFIPAGWTMPQFALRWI
                     LMHDAVTCTIPGAKNPAQAGDNVRAADLPSLSDEAMEKIRSVYESHIRSLVHNYW"
BASE COUNT          395 a          373 c          423 g          394 t
ORIGIN      
        1 ggatgttccc ccattatgac atataatgcc tccggtcaca aactgtgctt gcctcttttc
       61 aaaaaacatg cctcaacaga ttgatcaaaa catgattatc tccaacgtag caccgatcaa
      121 agcatcagtg tgatgttggc tgcactaaaa ggctgtagat ggtttcgatc acagcgtttt
      181 tcgaggctgc taaccaaaag aacctcagaa agtgaagggg aattcatgca tgtggttttt
      241 aggaaaaaaa atcacttcct gaaattgccc tttggatttc acttgaaaga ttttcgagcc
      301 cttcaaccaa ttcccagttt tcaaaatggc taatcgatgt tcctgggcgt aatcttgtta
      361 atggtcctag gctttcaagt tctacaaact gatgattgca gtacatttcg gcattgcaat
      421 tgttatcagg gtaaacgaga tcatcttgag cggagaatgt cttgcgaaac agaataccgt
      481 cgatatagta tgccagccaa ccatgtggat tgaaatatcc cagcttgaat gcgggcaggg
      541 catcagcttt gaaaatgaca tagtcatcac gcaggctcag gcgcgggtca ttgatgcgcg
      601 agtagggcca gaacgataga ttgcggttgg gtaaaagccc agcttcatcc acattcccga
      661 cgggcatagg cagaatgacg cttccgccta aattgaattg ggtgatcgcc cagggagcga
      721 gttccacagt ccataaacca tcattgatga gggtgtgggt gagtgtggcg gacggtttat
      781 ctggcgcgag ttgaatttcg atgcgtttgc gaatgcccgt gccgggctct gtttgcgttt
      841 caaggatcac gccattgggc aagtcggtga tcttcaactc gccggtatcg ggcgcgtagg
      901 tgcggggcat ggcttcgggc gcgtgccaca ggcgatgacc gccgcgaaaa taaaaatcgc
      961 cgtacggtgt tgggatgggc ggctggtggc tcatatctgc cagcagattc tttttgcctg
     1021 ttggagtcag ccccgagatg cgcaacgaat gggtgagata ttcaatttcg agaaagtcgt
     1081 ttttgagaat gcgtttgttc atgatttgat caccagtaat tgtgaacaag actgcggatg
     1141 tgcgactcat acacgctgcg gattttttcc atcgcctcat cactcagcga cggcaggtct
     1201 gccgcccgaa cattatcccc cgcttgagcc ggattcttcg cgccggggat ggtgcatgtg
     1261 acagcgtcgt gcatcagaat ccagcgcagg gcaaattgcg gcatcgtcca accagcaggg
     1321 atgaacttac gaagttcttc aacggcttgc aaaccggttt cataatccac accagagaaa
     1381 gtctcgccac ggtcgaatga ttcgccgtga cggttgaagg cacgatggtc atctttttca
     1441 aaggtcgatt tagcactgag cttgcccgtc aacagtccac tggcgagcgg cacacgcgca
     1501 agaataccca ccttgcggcg aatcgcttca gggaagaaga gttcagccgg acgttggcgg
     1561 aagatgttga aaatgatctg caccg
//
LOCUS       DAOQNJ010000616         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_73981_length_1584_cov_7.121648, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000616 DAOQNJ010000000
VERSION     DAOQNJ010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73981_length_1584_cov_7.121648"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1584)
                     /locus_tag="PLG52_13550"
     CDS             complement(<1..>1584)
                     /locus_tag="PLG52_13550"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB42526.1"
                     /translation="AIIGGLAKMLDYWEGEARADGVTEEVIQAVVSRLRSYEKLTPAG
                     RADSLKGLWKRIGETYPEAGQKPKGSGQPQTQNQPQRPPRGDAEENTKANGMDVVSAS
                     DQAQPRQNPPQQHKQRQAPPQRFEAATSAKHSQTPAALGAQLTVLQGVGPKNAESLEK
                     LGMRTLGDMLYYFPRRYEDYSLLKPIQALMYNDVVTVLGTIQSIHNRPIRGGKQNIIE
                     VIINDGTGSLRISFFNQPWLMNRLKQGEIISVSGKIDQYLGRIVMNSPDWESVEMENL
                     HTNRIVPIYSLTERVTQKWLRNQMKQVVTYWSPAVVDALPDITKREAQLVSLGEALTQ
                     VHFPDSQARLKLARERLAFDEIFYLQMGVLRQKRDWQEVEGRRFTVLDEWLGARLAAL
                     PFTLTSAQQAALEDIRKDLDSGKPMNRLIQGDVGSGKTVIAALGASIVVFNGAQAAIM
                     APTSILAEQHYRNFRNLLANEGGLLRPEQIRLLVSNTPDKEKEEIKSLLLTGEISIII
                     GTHAVIEPDVQFKDLQFVV"
BASE COUNT          369 a          431 c          370 g          414 t
ORIGIN      
        1 tcacaacgaa ttgcagatcc ttgaattgca catctggttc gatgaccgca tgtgtaccaa
       61 taattattga aatctcgcca gtcaacaata aactcttgat ttcttccttt tctttatcag
      121 gcgtattact aaccaacaaa cgaatttgtt ccggtcgaag taatccacct tcgtttgcga
      181 gtaggtttct gaaatttcga taatgctgtt ccgccagaat cgaagttggc gccatgatcg
      241 ccgcctgtgc cccattgaaa acgacaatac ttgcgcccaa tgcggctata accgtcttcc
      301 cggaaccgac atcaccttga ataagtcggt tcattggttt gccggaatcc aaatccttgc
      361 gaatatcttc gagggcggct tgctgggcag aggtcagggt gaagggcaag gcagctaaac
      421 gcgcacccag ccactcgtcc aagactgtga agcggcggcc ttccacttcc tgccagtcac
      481 gcttttgtct caacacaccc atctgcaaat aaaaaatttc atcaaaagcc agacgttccc
      541 gagcaagctt taaccgcgct tgtgagtccg ggaaatgcac ctgggtcaat gcctcgccca
      601 acgataccaa ctgcgcctcg cgtttggtta tatcgggcag ggcatctaca actgcgggtg
      661 accaatacgt aacaacttgc ttcatttgat tgcgcagcca tttttgggtg acccgttcgg
      721 tcaatgaata gattggcaca atacggttgg tgtgaaggtt ttccatctcc acactttccc
      781 aatcaggact gttcattaca atgcggccca ggtattgatc gatcttgcca gaaaccgaaa
      841 tgatctcacc ctgcttaagg cgattcatca accagggctg gttaaaaaat gaaatacgca
      901 gcgagccggt tccatcattg ataatgactt cgataatatt ttgcttgccg ccgcggatcg
      961 gtcgattatg aatgctttgg atcgtgccca acaccgtcac aacatcgtta tacatcaagg
     1021 cctgaatcgg tttgagcaat gaataatctt catagcggcg cgggaagtaa tacaacatgt
     1081 cgccaagcgt gcgcatgccc aatttctcaa gcgattcggc attctttggt cccacgcctt
     1141 gcaacacagt caattgagcg ccaagagctg ccggggtctg gctatgtttt gcactggttg
     1201 ccgcttcaaa tcgttgcggt ggagcctgcc tttgtttgtg ctgctgcgga ggattttgcc
     1261 tgggctgagc ctgatctgac gccgaaacca catccatccc attggccttt gtattctcct
     1321 ctgcatcgcc tcgcggcgga cgttggggct ggttctgtgt ctgcggctga cctgaccctt
     1381 taggcttttg acctgcttca gggtaggtct ctccaatacg cttccacaat cctttgagcg
     1441 aatccgcgcg cccggcgggt gtaagttttt cataggagcg caatcgtgaa acgacagcct
     1501 gaatcacttc ctctgtaacc ccgtcagcac gagcctcccc ttcccaataa tcgagcatct
     1561 tggcaagtcc gccgataatg gcag
//
LOCUS       DAOQNJ010000617         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_75336_length_1569_cov_7.771466, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000617 DAOQNJ010000000
VERSION     DAOQNJ010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75336_length_1569_cov_7.771466"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PLG52_13555"
     CDS             complement(<1..1008)
                     /locus_tag="PLG52_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNB42527.1"
                     /translation="MAVIYTPMFETLTGRLNQIFDNLRKRGKLSEADVDAAMKEVRLA
                     LLEADVHFSVVKTFIAKVRERAVGAEVSKALNPGQQVIKIVNEELVATLGEPAPLNLT
                     GPKPRVVMMVGLQGAGKTTVTGKLARLLKSKGERILMVAGDPYRPAAVKQLQQLGERV
                     GVDVEADPSIKPQELAKRALDKAEKGGYSLMLVDTAGRSQMDAQLMDELKAIHANVKP
                     VDTLLVIDSMIGQEALNIAQGFKETIDLTGLILTKMDGDSRGGAAISIRSVTGVPIKF
                     IGTGEKLDALETYDPSRLSSRILGMGDLIGLIEKAEAAYDAETAEKQAKKLMKGDFSL
                     ED"
     gene            1238..>1569
                     /locus_tag="PLG52_13560"
     CDS             1238..>1569
                     /locus_tag="PLG52_13560"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB42528.1"
                     /translation="MATEAIVNDRKEIFGWAMYDWANSAFSTTVGTVFLGPYVASLAR
                     AAAEAAGTSTVSFLGIPVAPDSFLPYCISFSVGMQVLFLPILGAIADYSHLRKTMMRI
                     FATIGAIAT"
BASE COUNT          366 a          394 c          401 g          408 t
ORIGIN      
        1 atcttcaagc gaaaaatcgc ctttcatcaa tttcttggct tgcttttctg cggtttcggc
       61 atcgtaggcg gcttcggctt tttcgatcaa gccgatgaga tcgcccatgc ccaaaatacg
      121 tgaagacaaa cgtgatgggt cataggtttc caatgcatcg agcttttcac ctgtaccaat
      181 aaatttgatc ggcacgccgg tcaccgaacg aatagagatg gcagcaccac cacgtgagtc
      241 gccgtccatt ttggtgagga taagacccgt caggtcgatg gtttctttga aaccttgagc
      301 aatgttcaaa gcttcctgac cgatcatgga atcgatgacg agcagagtat ccaccggctt
      361 gacattggca tggatggctt tcaattcgtc catcaactgc gcatccatct gggaccgacc
      421 ggctgtatcg accaacatca ggctgtagcc gcccttttca gctttatcga gtgcgcgctt
      481 cgcgagttcc tgcggcttga tggatgggtc tgcttccaca tccacgccca cacgttcgcc
      541 aagttgctgc aattgtttga ccgccgctgg gcgatatggg tcgccagcca ccatcaaaat
      601 acgctcgcct tttgatttga gcaaccgagc cagtttgcct gtaactgtcg ttttaccagc
      661 gccttgcaaa ccgaccatca tcaccacacg tggtttgggt cccgtgaggt ttagcggagc
      721 gggttcaccc agggtggcga caagttcctc gttgacgatc ttgatgactt gctgacccgg
      781 gttcaacgcc ttcgaaactt ctgcacccac cgcacgttcg cggactttgg caatgaaagt
      841 tttgaccacg ctaaaatgca catccgcttc gagcaaggcg aggcgcactt ctttcatggc
      901 ggcatcgaca tcggcttcgg agagctttcc gcgtttgcgc aaattgtcga agatttggtt
      961 gagacgtccg gtaagagttt caaacatagg ggtataaatg actgccatta agacaaaatg
     1021 gcaagattcc ttttcagtgt aagaagaggc attttagcgc ggagcaggtt gagggtcaag
     1081 caggaggcgc ggttcttaat ccataaaaat tggcaaaacc attcacagag acgagggttt
     1141 ttattctcac aatgttaacc gaacatctgt gggttgtccg tatataatac gggcagacgt
     1201 gaatccataa catcaatcca gttcgaggag aagaggcatg gcaacggaag cgatcgtcaa
     1261 tgaccggaaa gagatttttg ggtgggcaat gtacgactgg gcaaactcgg cttttagcac
     1321 gactgttgga accgtatttt taggaccgta tgtggcaagt ttggcgcgcg ctgcagcaga
     1381 agcggctgga accagcaccg tttcgttctt aggaattcct gttgcaccag attcttttct
     1441 accgtactgt atttcatttt ctgtaggcat gcaggttttg ttcctgccaa tccttggtgc
     1501 aattgcagat tactcgcact tgcgcaagac catgatgcgc atctttgcga ccatcggcgc
     1561 gattgccac
//
LOCUS       DAOQNJ010000618         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_75845_length_1563_cov_10.062997, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000618 DAOQNJ010000000
VERSION     DAOQNJ010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75845_length_1563_cov_10.062997"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PLG52_13565"
     CDS             <1..603
                     /locus_tag="PLG52_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNB42529.1"
                     /translation="FFTIFYNFAEGLISTYFGAQDETLTLFGFGVDSFIEVASGVGIL
                     AMIFRIRQNPNTSRSQFEQTALRITGTSFYLLAAGLGITGIYNLFTVHKPATTLPGLI
                     ISLVSITVMWALVLGKRKVGHALHSAPILADANCTMVCIYMSVILLASSLIYQFTGFG
                     RIDSLGAFGLIYFSINEGKEAFEKAANVNALCCDDDDCKE"
     gene            626..991
                     /locus_tag="PLG52_13570"
     CDS             626..991
                     /locus_tag="PLG52_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB42530.1"
                     /translation="MPIPLNTILIYAKNMKATAEFYQKYFGFVSTGEVVEGLIELTSA
                     HGGANILIHQAAKSIKLGQVGVKLMFDVEDIETFKKEAAAQGLKFGSTHQANGYSFAN
                     AKDPDKNPITISSRAYRRK"
     gene            1077..>1563
                     /locus_tag="PLG52_13575"
     CDS             1077..>1563
                     /locus_tag="PLG52_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNB42531.1"
                     /translation="MNSLQRIKNVIAGEPVDRTPNLDIFMIRAVHHLGIPLSKYYLDY
                     RVLCEVNMAALRDFELDVVQAISDPYREACDIGLEVEFPYDQMPMHRSLVIREPEDLA
                     KVKFPVQNFGPRMTDRLEGVRAMREQVGDEVAVMGWVEGAFAEAADLRGMTEIMKDVK
                     MR"
BASE COUNT          407 a          387 c          374 g          395 t
ORIGIN      
        1 ttcttcacca tcttttataa ctttgccgaa gggcttatct ccacctattt tggcgcgcaa
       61 gatgagacgc tgacactctt tggctttggc gtcgactcat tcattgaggt tgcatcggga
      121 gtcggcatcc ttgccatgat atttcgcatc cgccaaaacc ccaatacctc ccgttctcaa
      181 ttcgaacaga ccgccttgcg cattacaggc acatcctttt atctgctggc tgccggtctt
      241 ggcatcacag gcatttacaa tctttttacg gtccacaagc cagctacaac tctgccgggg
      301 ctgatcattt cattagtttc catcacggtc atgtgggcat tggtgctggg gaagcgtaaa
      361 gtagggcatg cgcttcattc cgctcccatt ttggcagatg ccaactgcac catggtttgt
      421 atttacatgt ccgttattct gttggcatcc agccttatct atcaattcac aggctttggg
      481 cgaatcgaca gtctcggcgc ctttggtcta atttacttct ccatcaacga aggcaaagaa
      541 gcctttgaaa aagcagccaa tgtcaacgca ctttgttgtg acgatgatga ttgcaaagaa
      601 tagaagcgta ggaacagaaa atcgaatgcc aatccccctc aataccatcc tgatttatgc
      661 aaagaatatg aaagccactg cagagtttta tcaaaaatac tttggctttg tttcaaccgg
      721 cgaagtggtg gaagggctta ttgaattaac gtccgcgcat ggcggggcaa atattctgat
      781 tcatcaggca gccaaaagca taaagttagg tcaagtcggc gtaaagctca tgttcgatgt
      841 tgaagacatt gaaacattca agaaagaagc ggcggcacaa ggtcttaagt ttggcagtac
      901 ccatcaagca aatggttatt cttttgcaaa cgccaaagac ccagacaaaa acccgattac
      961 catttccagc cgtgcttatc ggcgaaagta gggtttcaga taaaaatact gggcgcatac
     1021 gccaagtctg cgcattgggg gtatcataga aaaaaatcct ctcacaaggt ggtcttatga
     1081 actctctgca acgcataaaa aatgtcatcg ctggcgagcc ggtggaccga acccccaacc
     1141 tcgatatctt tatgatccgc gccgtgcatc acctcggcat tccgttatcc aaatattatc
     1201 tggactatcg cgtgttatgt gaagtcaaca tggcagcctt gcgagatttc gagctggatg
     1261 tggtgcaagc catttcagac ccctatcgtg aagcctgcga tatcgggctg gaagtcgaat
     1321 tcccatatga ccaaatgccc atgcacagaa gcctggtcat ccgtgagccg gaagatctcg
     1381 ccaaggtcaa attcccggtg cagaactttg gtccacgcat gacagaccga ttggaaggcg
     1441 tccgcgccat gcgagagcag gttggggatg aagtcgctgt gatgggctgg gtcgaaggcg
     1501 cctttgcaga agccgcagat ttgcgcggca tgaccgagat catgaaggac gtaaaaatgc
     1561 gcc
//
LOCUS       DAOQNJ010000619         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_77119_length_1549_cov_9.050201, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000619 DAOQNJ010000000
VERSION     DAOQNJ010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77119_length_1549_cov_9.050201"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..>1549)
                     /locus_tag="PLG52_13580"
     CDS             complement(123..>1549)
                     /locus_tag="PLG52_13580"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNB42532.1"
                     /translation="RVISAPELDALEKADYDTVEGFRKAAWEAYLAPLTEERNQVMDI
                     LEEIASSTPNHVAELRMLRDRLTALPSFSRRDIHSIAHEALRLLRNESHPSKQVLIQW
                     KHENDKLTEERYSSHLYSGTALKVEEIKPVYSDSSPTMFGFEVINAAFDAALTRDSKL
                     IAFGEDVGKLGDVNQGFKGMQEKYGEFRVTDTGIREATIMGQAIGMAMRGLRPIAEIQ
                     YLDYVLYALQTMSDDLASLHWRTAGGQKAPVIVRTRGHRLEGVWHAGSPMSGILSLTR
                     GMYVLVPRDMTRAAGFYNTLLKSDEPAIVVEVLNGYRVKEKLPDNIGEITLPLGVPET
                     IRQGKDITIVTYGACCRIVLDAAEKLSKVGIDVEVIDVQSLMPFDIHGKIVESLKNTN
                     RILFVDEDVPGGATAYMMQEVIEKQGGYFHLDSPARTLSAKAHRPSYGSDGDYWSKPN
                     AETVFDVVYEMMHEVNPTAFPLFS"
BASE COUNT          402 a          394 c          354 g          399 t
ORIGIN      
        1 aattcattaa tagaatcacc aacttgtttg agccatttgg tgaccgtaac ttcgtccacg
       61 ccttcgccga gccggggtac ataaactttt gtagccatga atttctccag ttggtcgtgc
      121 tatcaactaa aaagtggaaa tgcagtcgga ttcacttcat gcatcatctc gtacaccaca
      181 tcaaacacgg tctcagcatt gggcttgctc cagtaatctc cgtctgagcc atatgatgga
      241 cgatgcgctt tggcagataa tgtacgtgcg ggcgagtcta aatggaaata accgccttgc
      301 ttctcgatga cttcttgcat catataggca gttgccccac cgggaacatc ttcatctacg
      361 aagagaatac ggttggtgtt ctttaacgat tccacgatct tgccatgaat atcgaagggc
      421 atcagcgatt ggacgtcgat cacttccaca tcgattccta ccttgctcaa tttctcagca
      481 gcatccaaca caatacgaca gcacgcccca taagtgacga tggtaatgtc tttgccttgg
      541 cgaatggttt caggaacacc caagggtaat gtaatttcac caatgttatc gggcaatttc
      601 tctttgacac gatacccatt tagaacttca acaacaatag ccggctcatc cgatttcaat
      661 aaggtgttgt agaaaccagc ggcgcgggtc atatcgcgcg gaaccaaaac atacatgccg
      721 cgagtcaaac taagaatgcc agacatgggc gagcctgcat gccaaacacc ttccaaacga
      781 tgcccacgtg tgcggacgat aacaggcgct ttctgtccgc cagcggtgcg ccaatgcaat
      841 gaagcaaggt catcactcat ggtttgcaac gcatacaaga cataatcaag gtattgaatt
      901 tcagcaatcg ggcgtaatcc gcgcattgcc atgccaatgg cttgtcccat aatggtagct
      961 tcacgaatac cggtatcggt cacacggaat tcgccgtatt tttcttgcat ccccttaaaa
     1021 ccctgattga catcgccaag cttgccaaca tcttcaccga aagcaattag ttttgagtca
     1081 cgggtaagtg ctgcatcaaa ggctgcattg atcacttcaa aaccaaacat ggtgggggag
     1141 ctatctgaat aaaccggctt gatttcttcc actttgagtg ctgtgccaga gtacaaatgc
     1201 gaactatagc gctcttcggt cagcttgtca ttttcgtgct tccattgaat gagaacttgt
     1261 ttcgaaggat gagactcatt tcgtagaaga cgcaatgcct catgcgcaat ggaatggata
     1321 tcgcgcctcg aaaacgacgg gagggctgtc agccgatctc tgagcattcg caactctgca
     1381 acatgattcg gagtcgagct ggcaatctct tcgaggatat ccatcacctg attgcgttct
     1441 tcggtcaacg gtgcgagata agcttcccat gcagctttgc ggaatccttc tacggtgtcg
     1501 tagtctgctt tttcgagggc atccagctcg ggcgcggaaa tgacgcggt
//
LOCUS       DAOQNJ010000620         1548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_77226_length_1548_cov_6.098459, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000620 DAOQNJ010000000
VERSION     DAOQNJ010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77226_length_1548_cov_6.098459"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..>1548)
                     /locus_tag="PLG52_13585"
     CDS             complement(71..>1548)
                     /locus_tag="PLG52_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB42533.1"
                     /translation="QGNAIIRRNLMKLRSSFLLLILLISACSLPAATQPTATSTPQPT
                     EQATETPTQAPTTEPPKIPDHASYILDTVIDYDLHRVSVTENIQYPNHTGQKLTSLTL
                     AIAANLWADCFHLAEVSVDGIPLSNYTLNQHRLDLPLQTPLAPDSVLTVTLRYSLSLP
                     YMDQVNSQRARIFGYSDIQMNLVNWYPFIVPFKEGEWIIREPWSHGEYLVYPTADFEV
                     NLKFANPENQPIVAASGFAEPNGDFTRYTLSNGRAFAISASREFQVSQTQVDDVTVYS
                     YYLPIYKNAGEAAMNASAQALQVFSQRFGKYPHKTLSVVIADFKDSMEFSALYFHSRS
                     FYDLYDGTVANYLTTVAVHETGHQWWFDQVANDQAEEPWLDESLTTYSELIYYETLHP
                     DLVSWWWSYRIDFFQPQGKIDIPVYEGQNADTYKVIVYFNGAHFLKDLRERIGDEAFF
                     AFIQDYCAQEKNNIATSDDFFRILDEHTDVDYSDIVRTYFK"
BASE COUNT          390 a          306 c          442 g          410 t
ORIGIN      
        1 tgggtgttgg gattgtactc tttacagatg atagacgata gatgatggtt tatggacggt
       61 ggaacatgat ctatttaaag tatgttctga caatatctga gtaatcgaca tccgtatgtt
      121 catctaaaat gcgaaaaaaa tcgtcgctag tggcgatgtt gttcttttct tgtgcgcagt
      181 agtcttgaat aaaggcgaag aaagcttcat cgccaatgcg ttcgcgcagg tctttaagaa
      241 aatgagcgcc gttgaaatag acaatgacct tatacgtatc tgcgttttga ccttcataga
      301 cgggaatatc gatcttgcct tgcggttgga agaagtcaat gcggtacgac caccaccacg
      361 aaacgaggtc tgggtgcagg gtttcgtagt agatcaattc agaataggtt gtgagcgatt
      421 cgtcgagcca gggttcttcg gcttggtcat ttgccacttg atcgaaccac cactgatgcc
      481 ccgtctcatg gacagccaca gtggtgaggt aattcgctac cgttccatca tagaggtcgt
      541 aaaaactgcg gctgtgaaaa tacagtgccg aaaattccat gctgtctttg aaatcggcga
      601 tgaccacgga aagagtcttg tgcggatatt tgccaaagcg ttgtgaaaag acttgcagag
      661 cttgcgcact ggcgttcatt gcggcttcac ctgcattttt ataaatgggc aagtaataac
      721 tgtagacagt cacatcatcc acttgcgttt gagagacctg aaactcgcgg ctggcagaga
      781 tggcgaaggc acgcccgttt gaaagtgtgt aacgggtaaa gtcgccgttc ggttcggcga
      841 atccgctggc ggcgacgatg ggttgatttt cgggattcgc aaatttcaaa ttgacttcaa
      901 aatctgcggt ggggtaaacc agatattcgc catgcgacca cggctcacgg atgatccact
      961 cgccctcttt gaatggcaca atgaacggat accaattgac caagttcatt tgaatatcgc
     1021 tataaccaaa aatgcgtgcg cgctggctgt tgacttggtc catgtagggg agtgaaaggc
     1081 tatagcggag agtcactgtc aaaacagaat caggcgcgag cggagtctga agcggaaggt
     1141 cgagtcgatg ttggttgagt gtgtagttgc ttagcggaat tccatccacg ctgacttcgg
     1201 caagatgaaa acaatcagcc caaagattag cggcgatggc gagggtgagg gaggtgagtt
     1261 tttgcccggt gtgatttgga tattgaatgt tctcagtcac gctgacgcga tgtaaatcgt
     1321 agtcgatcac ggtatcgaga atgtagctgg catgatctgg aatttttggc ggctctgtag
     1381 ttggagcttg cgtgggcgtt tctgtggctt gctctgtggg ttgcggcgtg gaagttgctg
     1441 tgggttgtgt tgctgcggga agcgaacagg cagagattaa gagaatcaat aacaggaagg
     1501 atgaacgcag tttcataagg ttgcgccgaa ttatagcgtt cccttgat
//
LOCUS       DAOQNJ010000621         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_77791_length_1542_cov_8.154001, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000621 DAOQNJ010000000
VERSION     DAOQNJ010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77791_length_1542_cov_8.154001"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..842
                     /locus_tag="PLG52_13590"
     CDS             198..842
                     /locus_tag="PLG52_13590"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNB42534.1"
                     /translation="MVTKEMNINMAPNLAFAKLASEAQIQKMTKALEANGIKVLIAET
                     GEEAKNMVLGLVPQGAEVYSNMSKTLDTLGLSAEFDKSGRYNAVRPRVLALDRKTQAD
                     EIRKLRTIPDYIVGSVHAITEAGEVLTASGTGSQISSYAYGAGKVIWVVGTQKIVSDL
                     SEGFRRIKEYSHPLEDARMQEAYGVNSSLNKILVLSRDMPGRITIVLVKEELGF"
     gene            923..>1542
                     /locus_tag="PLG52_13595"
     CDS             923..>1542
                     /locus_tag="PLG52_13595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB42535.1"
                     /translation="MSTQTNSPAKQEYSVHPATRMGHVALTVANLENQILFYEKAIGL
                     KLHWREGNKAGLGAGGADLLQLTEEPNLKRYRGGTGLYHFAIVFPNRRELAIAMARLF
                     ALKVPNSPTDHVMTKTTYLSDPEGNGIELYCESPEDGSWTIKDGNYEARWADGRWSDG
                     REALDVDALFTHLKEDDKLDAPVPAETRMGHVHLHVRDIPEAVDFY"
BASE COUNT          429 a          378 c          387 g          348 t
ORIGIN      
        1 cgccgtggat gcaggtccgc tgagccgcgc ccgccagatc gaaggcatgc aattgttgca
       61 cattgtattg cagggaactc tcggcaccaa ttggggcagt gcgcttaaga ttattgcgta
      121 gtcttttgat aaaagcttat ggcacttgca taattcaaat gtaagtgcca tgtatgctgt
      181 aagaaaagaa ggttcgaatg gttacaaaag aaatgaatat caacatggct ccaaaccttg
      241 catttgctaa attagcaagt gaggcgcaaa ttcaaaaaat gacgaaagcc ttggaagcca
      301 atggaataaa agtacttatt gccgaaacgg gtgaagaagc caagaacatg gttctgggtt
      361 tggtcccgca aggcgcggaa gtatattcca acatgtcaaa gacgctcgat acgcttggct
      421 tgtctgccga gttcgataaa tctgggcgtt ataacgcggt ccgcccgcgt gtgctggcgc
      481 tagaccgtaa gacacaagca gacgaaatcc gcaagctacg cacgattcca gattatattg
      541 ttggcagcgt ccacgcgatt acagaagcag gtgaagtgct tacggcttca ggcacaggca
      601 gccaaatttc ctcctatgcc tatggcgcag ggaaagtgat ttgggtggtc ggaacacaaa
      661 agattgttag cgatctaagc gaaggcttcc gccgcatcaa agaatacagt catccgcttg
      721 aagatgctcg catgcaagaa gcctatggcg tcaacagttc gctcaacaaa atattggttc
      781 taagccgcga tatgccaggg cgcatcacca ttgttctggt gaaagaagaa cttgggtttt
      841 agcccgaacc aaagaaagta tttccgtgtt taagtcatct agtacatagg cttagaatca
      901 tggacgaaag ataaaggtat atatgtcaac tcaaacaaac tcccccgcaa agcaagaata
      961 ctcggttcac cccgccacca gaatgggaca cgtcgctctc accgttgcca atttggagaa
     1021 tcagattctc ttttatgaaa aagccatcgg gttgaaactg cactggcgcg aaggcaataa
     1081 agccggtctc ggagccggag gcgcggatct acttcaactt actgaagaac cgaatctcaa
     1141 acgctatcgt ggcggcacgg gcttgtatca ctttgccatc gtatttccca atcgccgcga
     1201 gcttgcgatt gccatggcgc gtttgtttgc gttgaaagtt cccaactcac ccaccgacca
     1261 tgtcatgacc aagaccacct acctcagtga ccctgaaggt aacgggatcg aactgtattg
     1321 cgaatcgccc gaagatggtt cgtggacgat caaagacggc aactatgaag cccgctgggc
     1381 agacggacgc tggagcgatg gacgcgaagc actcgacgtc gatgcgctct tcactcatct
     1441 caaagaagat gacaagcttg atgcccccgt ccctgccgaa acccgcatgg gacatgtcca
     1501 cttgcatgtg cgcgatattc ccgaagctgt cgatttttat ca
//
LOCUS       DAOQNJ010000622         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_78138_length_1538_cov_8.860418, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000622 DAOQNJ010000000
VERSION     DAOQNJ010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78138_length_1538_cov_8.860418"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLG52_13600"
     CDS             complement(<1..764)
                     /locus_tag="PLG52_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42536.1"
                     /translation="MKRFETPYMADWFAISLRWIMLVGLIVSLGLGQRLDASASWPLG
                     LMIVWNLGMTALAGLNIRTQYHRRLNIAMDLILTAAFFWAQGGVRGPAFWAGLLPILT
                     GSIYFELIGAVVAAILFSGFVIYTGTRVDGNITLAFLVAAILSLLGVLFGFLGRRLTD
                     HMRKNRALWVDAEEKKRAIQNERMRAIYELTSTLSTTLSYKRVLDSALDMSASALNPN
                     LDPEQTTSDPLVGAVMLFNAGKLRIGSARRFTNADMR"
     gene            complement(843..>1538)
                     /locus_tag="PLG52_13605"
     CDS             complement(843..>1538)
                     /locus_tag="PLG52_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNB42537.1"
                     /translation="GSGWHYNLIHDLMKTTGCADAQDIRKKYGLQKILTEVNCNIGVL
                     SPRRLAMVTVPAVLGNTFVIKAHASPSSASRFLASLGMLRITYIYRDPRDAMLSAFDY
                     GQRALAKGRPNAFSHLSDFEKSVDFMMEYVHIWEKWMKEKNVLIARYEDLLTNYEVES
                     AKLVDYLKLDANKPEVRAVIEQYRPGANDGQQGLHFYKGKIGRFRDAYTSEQQNVLLE
                     KVSPYLERMNYEK"
BASE COUNT          426 a          398 c          362 g          352 t
ORIGIN      
        1 cgcatatcag cgttggtgaa acgacgagcg gagccaatgc gtaatttacc cgcattaaaa
       61 agcataacag cgcccactaa cggatcgctg gtagtttgtt ctgggtcaag attgggattc
      121 aatgcagaag cgctcatatc gagcgccgag tcaagcacac gtttatagct aagggtcgtg
      181 gaaagcgtgg aagtcaattc gtagatcgca cgcatacgtt cattttgaat ggcacgtttc
      241 ttttcttcgg catcaaccca caaagcccga ttcttgcgca tatggtcagt tagccggcga
      301 cctagaaacc caaagagtac ccctaaaagg ctcagaatcg cagctacgag aaatgcaagg
      361 gtaatattcc catccacccg ggtgccggtg tagataacaa agcctgagaa caaaatggca
      421 gccaccactg cgccaattag ctcaaagtag atggaaccgg tcaaaatagg gagcaatcct
      481 gcccaaaaag caggtccccg aacgccacct tgcgcccaga agaatgccgc tgtaaggatc
      541 aaatccatcg caatgttgag gcggcgatga tattgtgtac ggatgtttaa gccagctaga
      601 gccgtcatcc ccaagttcca aacaatcatg agccccagag gccagcttgc actcgcatct
      661 aatctctgtc caagcccaag agaaacgata agacccacta gcatgatcca gcgcagtgag
      721 atggcaaacc agtctgccat gtagggagtt tcaaatcgtt tcatcgctgc cctatgataa
      781 cgaaaaaggg atgtttcgac aacatccccg acgttacagt cagattacac ctagtgcaaa
      841 tattactttt cgtagttcat acgctccaaa tacgggctta ctttctctaa aagcacgttt
      901 tgctgctcgc ttgtatacgc atcacggaaa cgcccgatct tgcccttgta aaaatgcaag
      961 ccttgctgcc catcatttgc gccagggcga tattgttcga tcaccgcgcg gacctcaggc
     1021 ttgttcgcat ccaacttgag ataatccacc agtttggcag attcaacttc atagttggta
     1081 agtaggtctt cgtaacgcgc gatcaacaca tttttctctt tcatccactt ttcccaaatg
     1141 tggacatatt ccatcataaa atccacgctt ttttcaaaat cggaaaggtg tgaaaaagcg
     1201 ttgggacgtc ccttggcaag agcacgctga ccgtagtcga aggcagagag catggcatca
     1261 cgtggatcgc ggtagatgta ggtaatacgc agcataccca gtgaagccaa gaagcgggaa
     1321 gccgatgaag gactcgcatg cgccttgatc acaaaggtat tgcccagtac ggctggcaca
     1381 gtcaccatcg ccaaacggcg cggagaaagc acaccaatgt tgcaatttac ctcggtgaga
     1441 attttttgca gcccatattt tttacggatg tcctgcgcat cggcacagcc cgtggttttc
     1501 atcaggtcgt gaatgaggtt atagtgccag cccgaccc
//
LOCUS       DAOQNJ010000623         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_78424_length_1535_cov_7.589865, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000623 DAOQNJ010000000
VERSION     DAOQNJ010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78424_length_1535_cov_7.589865"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLG52_13610"
     CDS             <1..243
                     /locus_tag="PLG52_13610"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNB42538.1"
                     /translation="MISFIMKDGRDAAVKVAESTKLFTLAESLGGVESLIEVPAAMTH
                     LSTQGSTLEVDPGLVRLSVGIENVDDLIADLEQALR"
     gene            complement(274..>1535)
                     /locus_tag="PLG52_13615"
     CDS             complement(274..>1535)
                     /locus_tag="PLG52_13615"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB42539.1"
                     /translation="RYQIMNEKMDSSVFLKIPLFKDLPKAELYSLVSELPVEKYQAGD
                     YIFHEGEPGNSLYVVQKGKIDVVLAGGTNDEMLLRTCGPGEYVGEMSLILKQGKRTAA
                     VRVREDSQLWMMTRDKFNECLQRWPHLAYAMVEIISERLDSSNEAAFNDLVKKNQMLQ
                     KAYDDLKAAQAQLIEKERLERELQLAADIQLSILPDVLPDTEEFGFGARILPARQVGG
                     DFYDVFQLKDDRIGVLIGDVADKGVPSALFMARAHALIMAEADIGATPAEVMNLVNSH
                     ITRLQKSTQFVTVLYGILDLKTRIFSYARAGHEPPLVLHTDGSVERIPHSPGMAIGLW
                     EPVTLDEKTVKLSSGDTLLLYTDGMTDCRDPQGDAFGLERIKDKLGELSKQSAQQICN
                     TLLETLQNHQNGAKQDDDVTLVAVKAK"
BASE COUNT          367 a          413 c          366 g          389 t
ORIGIN      
        1 atgatctcgt tcatcatgaa agatgggcgt gatgcagcgg tcaaagtcgc agaatccacc
       61 aagctattca cgttggcaga atccctcggc ggcgtggagt cgcttatcga agtcccggcg
      121 gcgatgacgc atctctccac acaaggttcc acgctcgaag ttgaccccgg cttggtacgt
      181 ctctccgtgg gcatcgaaaa cgtggacgat ctgatcgcag accttgaaca agccttacgc
      241 taaaacgaag acctcccttg cgggaggtct tttttatttc gcttttacgg caaccaaagt
      301 cacatcgtca tcttgcttgg cgccattctg gtgattttgt agggtttcaa gcaatgtatt
      361 gcaaatctgt tgtgcgcttt gcttagaaag ttcgccaagt ttatccttga tgcgttccaa
      421 gccaaatgcg tcgccttgtg ggtcgcggca gtcggtcatg ccgtcggtat ataagagcaa
      481 ggtgtcgcca gaagataact tgaccgtctt ctcgtccaac gtgaccggct cccataaccc
      541 gatcgccatg ccggggctat gtggaatacg ctccacgctt ccatcggtat gcaataccaa
      601 cggcggctca tgcccagcac gtgcatatga aaagatgcga gtcttcaaat ccaaaatgcc
      661 atagagcacg gtcacaaact gcgtggattt ttgcaagcgc gtgatgtgcg aattgaccaa
      721 gttcatcacc tcggcaggag tcgcaccaat atccgcctct gccatgatga gcgcatgggc
      781 acgtgccatg aacagcgcag aaggcacacc tttatcagct acatcaccga tcaaaacccc
      841 aatgcggtca tccttgagtt ggaagacatc ataaaaatcg ccgcctacct gacgggcagg
      901 caaaatccgt gcgccgaaac caaactcttc ggtatcgggc aaaacgtctg ggagaatgct
      961 aagctgaata tcagctgcca attgaagttc acgttcgagg cgttcttttt cgatcaactg
     1021 ggcttgggct gctttcaagt catcataggc tttttgcagc atctgattct ttttcacaag
     1081 atcgttaaaa gctgcttcgt tcgaagagtc caaacgttcg ctgatgatct cgaccatggc
     1141 atatgccaaa tgaggccagc gttgcagaca ttcattgaac ttatcgcggg tcatcatcca
     1201 aagctggcta tcttcccgca cgcggacagc ggctgtgcgc ttgccttgtt tgagaatgag
     1261 actcatctca cccacatatt cgccgggtcc acaggtgcgt agaagcattt catcgttcgt
     1321 gccgcccgcc agaaccacgt cgatcttgcc tttttgaacc acgtataaac tattgcccgg
     1381 ctcaccttca tggaagatgt aatcgcccgc ttgatatttt tctaccggca gttcgctgac
     1441 caaggaatac aactccgcct tgggcaggtc tttgaacagt ggaatcttta gaaagacgga
     1501 actatccatc ttttcattca ttatttggta tctcc
//
LOCUS       DAOQNJ010000624         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_78611_length_1533_cov_7.737483, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000624 DAOQNJ010000000
VERSION     DAOQNJ010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78611_length_1533_cov_7.737483"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PLG52_13620"
     CDS             complement(<1..837)
                     /locus_tag="PLG52_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42540.1"
                     /translation="MRIAIITTHPPSKGSLNEYAYHFVRFLKTKVETVSEIVLLLDEL
                     PQGEEYPSEQRQAQVPVRSVPCWKFNAMDNVLRIRRAVREANPDIVLFNIQFASFGDK
                     KVPATLGLLTPWVLKLSGHKVVVLLHNIMETVNLKSAGFGSNPILEAIIRLFGNIITR
                     FILTADWVAVTIPKYVEILESKYGAQNVLLAPHGSFEEPTNTEPSTMPGGCPTVMTFG
                     KFGTYKTVDLLVEAHAELLKKHPEMRLVIAGTDSPNAAGYLQGVRDKYAHVPNVEYTG
                     YVA"
     gene            complement(847..>1533)
                     /locus_tag="PLG52_13625"
     CDS             complement(847..>1533)
                     /locus_tag="PLG52_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB42541.1"
                     /translation="YNERERIVATIGAIASHVSDLGIPWELLIADDGSKDDTVALCQD
                     LHLANMRVLIAPKNGGKGSAVQRGMLAARGRYVLFADADNSTPIEEVSKLLAKLEKEG
                     FDVAVGSRAADGAEEGKKSLLRQVLSNGLRWIVKYIFFIPVKDTQCGFKMYTRETAQK
                     LHTKQTIMGFSFDLEILYLASKYGYKIAEVPVSWIDAPGSKVDTRKEVQRFLRDLMTV
                     KMNDLKGVYR"
BASE COUNT          395 a          444 c          336 g          358 t
ORIGIN      
        1 tgccacatag ccggtatatt ccacattagg cacgtgggca tatttatcgc gcaccccctg
       61 caagtaacca gccgcattgg ggctgtccgt tccggcaatc accaggcgca tctccggatg
      121 cttcttcaat aattcggcat gcgcctccac caacaggtca acagttttat acgtgccaaa
      181 ctttccaaag gtcatcacgg tcgggcagcc gcccggcatg gtgctgggtt cggtattcgt
      241 tggctcctca aaggaaccat gcggcgcaag cagtacattt tgtgcgccat atttactttc
      301 caaaatttcc acatacttcg gaatggtcac tgcaacccaa tcggctgtca aaataaaacg
      361 cgtaatgatg ttcccaaaca aacgaatgat ggcttcaagg atggggttgc tgccaaaacc
      421 agccgatttc aaattgaccg tttccatgat gttatgcaac agcaccacca ccttgtgccc
      481 gcttaatttc aagacccaag gcgtcagcaa ccccaaggtg gctggcactt ttttatcacc
      541 aaaagaagca aactgaatat tgaacaaaac aatatcagga tttgcttcgc gaacagccct
      601 gcgaatacga aggacattat ccatcgcgtt gaacttccaa cacggcacgc ttcgcaccgg
      661 aacttgggcc tgacgctgct ccgagggata ttcctcaccc tgaggcagtt cgtccaatag
      721 cagaacgatc tctgaaaccg tctccacttt cgtctttaaa aagcggacaa aatgatacgc
      781 atactcgtta agcgagcctt tggaaggcgg gtgggttgtg ataatcgcaa ttcgcataaa
      841 aacctcctaa cgataaacgc cctttaagtc gttcatcttc actgtcatta agtcacgcaa
      901 gaagcgttga acttccttgc gcgtgtccac tttcgaacca ggcgcatcga tccacgagac
      961 cggcacttcg gcgatcttat aaccatactt tgaagcgagg tacaaaatct ccaggtcaaa
     1021 cgaaaaaccc ataatggtct gtttcgtgtg caacttttgg gcagtttcac gtgtgtacat
     1081 tttgaaaccg cattgcgtat ccttgaccgg gataaagaag atgtacttca cgatccaacg
     1141 caaaccattg ctcaaaacct gccgcaacag agactttttg ccttcctctg cgccgtccgc
     1201 cgcccgcgaa ccaacagcga catcgaagcc ttctttctca agcttggcca gcagcttgct
     1261 cacctcttca atgggcgtgg agttatccgc atcagcaaag aggacatagc gcccgcgagc
     1321 cgccaacatg ccgcgctgca cagcactgcc cttgccgcca ttcttcggcg caataagcac
     1381 tctcatgttg gctaaatgca gatcctgaca caatgccacc gtatcatcct tcgagccgtc
     1441 atcggcaatc agcaattccc acggaatgcc cagatctgag acatgcgatg cgatcgcgcc
     1501 gatggtggcc acgatccgtt cacgctcgtt gta
//
LOCUS       DAOQNJ010000625         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_78924_length_1530_cov_9.235932, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000625 DAOQNJ010000000
VERSION     DAOQNJ010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78924_length_1530_cov_9.235932"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLG52_13630"
     CDS             <1..693
                     /locus_tag="PLG52_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB42542.1"
                     /translation="GGISIRNYYATSLPEGVNKPFARQGEMIDYFSELFGPYPFEVYG
                     ALVMDTEFGAALENQTMSIFGIDMVDLEDIEGTEAVVAHELVHQWFGDSVSVGDWGSI
                     WLNEGFASYGEGLWEEHLNGRDALDEWVNGVYEEVKAYPEFYPPPGNPPANDLFNGGV
                     YLRGGLTLHALRLEVGDDVFFEILRTYYDRYKYGNAVTDDFIAVAEEVSGKDLGDFFN
                     AWLYEEQLPELP"
     gene            complement(777..1421)
                     /locus_tag="PLG52_13635"
     CDS             complement(777..1421)
                     /locus_tag="PLG52_13635"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HNB42543.1"
                     /translation="MTASPLFEFALLSFISMFTMVNPIGVVPVFTAMTAKLTPQESHR
                     VARKATFTGAVILFAFALTGQWIFRFFSISVDSLRVVGGVIFFFMGYEMLQARLTRTQ
                     FDDETNHEYINDISITPLGIPIICGPGAITNAILLMNEADTMVEQGIVLGVIAIILLF
                     TYLLLLGAKQVTRIIGENGNKVMLRLMGLIVMVIAVEFFFAGLTPIVRKMLSGG"
BASE COUNT          416 a          359 c          382 g          373 t
ORIGIN      
        1 ggtggtattt cgatacgcaa ttattacgcc accagtttgc ccgagggtgt caataaacca
       61 tttgcgcgtc agggcgagat gatcgactac tttagcgagc ttttcggtcc gtatccattt
      121 gaagtgtatg gtgctttggt gatggacaca gagtttggtg ccgcgcttga aaaccaaacc
      181 atgtccattt ttggaatcga catggtggat ctggaagata ttgaaggcac agaagcagtg
      241 gttgctcatg aacttgtcca tcaatggttt ggtgatagcg tcagtgttgg cgattggggc
      301 agtatttggc tcaacgaagg ttttgcttca tatggcgaag gtctttggga agaacacctc
      361 aacgggcgcg acgcacttga tgaatgggtt aatggtgtgt atgaagaagt gaaggcatac
      421 ccagagtttt accctccgcc tggcaacccc ccagccaacg atctcttcaa tggcggcgtt
      481 tacttgcgcg gcgggctcac gctccacgcc ttacggcttg aggtcggcga tgatgtgttc
      541 tttgagattc tgcgcacata ttatgaccgt tataaatatg gcaacgccgt caccgatgat
      601 ttcatcgctg ttgctgaaga agtcagcggc aaagacctcg gcgatttctt caatgcctgg
      661 ttgtacgaag aacaacttcc tgaacttcca taggataaaa gagacagtcc catgtaatgg
      721 gactgtctct tttacgcttc caacttaact caaacctgct ttagcgctcc gagcctttag
      781 ccacccgaaa gcatttttcg tacgattggt gtcaacccgg cgaagaaaaa ttccacggcg
      841 atcaccatta cgattaaccc catcaaacgc aacatgacct tattcccatt ttccccaata
      901 atgcgtgtga cttgttttgc gccaagtaat aataaatatg taaaaagcaa aataatagcg
      961 atcactccca acacaatgcc ttgttctacc atcgtatcgg cttcgttcat caaaaggata
     1021 gcattggtaa tggcgcccgg tccgcagatg atgggaatgc ccaacggcgt aatggaaata
     1081 tcgttgatgt attcatgatt ggtttcatca tcaaattgag tccgcgtgag gcgggcttgc
     1141 aacatttcat aacccataaa gaaaaagatc acaccaccca ctacccgtaa actgtccaca
     1201 gagatcgaga agaagcgaaa gatccactga ccggtcaacg cgaaggcgaa gagaatcacg
     1261 gcaccggtaa aggtcgcttt gcgtgccacc cgatgcgact cttgcggagt tagcttggcg
     1321 gtcatggcag tgaagacagg tacaacccca atcgggttga ccatggtgaa catcgaaata
     1381 aagctgagca gggcaaattc aaaaagagga gatgcggtca tggcgttgat tttacccgca
     1441 cttgacagga gcctgattcc gcagataatt acccacacaa aggcagtgac agaggaaagt
     1501 aggctgacgg aagccgccag agatgtgcaa
//
LOCUS       DAOQNJ010000626         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_79737_length_1522_cov_7.211997, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000626 DAOQNJ010000000
VERSION     DAOQNJ010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79737_length_1522_cov_7.211997"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..687
                     /locus_tag="PLG52_13640"
     CDS             70..687
                     /locus_tag="PLG52_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNB42544.1"
                     /translation="MSVRNAILGLLAQRPRHGYELHSAFSTVVGGATWDVKPAQIYTT
                     LERLEESGLVQTKSDLGEGREPDRRIYAITRNGHEALKSWFNEGVPTEHQRDEFFIKL
                     MVGLISGEADPARIIQTQRSRLFQEMHDATTQRDTYDPRIEMAQILLVDKAIMHLEAD
                     LRWLDMIEMRLETIKDQPIPEPETRKRGRPKKIDAGSTYPPPSEK"
     gene            769..1512
                     /locus_tag="PLG52_13645"
     CDS             769..1512
                     /locus_tag="PLG52_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB42545.1"
                     /translation="MSNIQIEKLTKTYGSGVTAVAALDHVNLTIREGEFVAVMGPSGC
                     GKSTLLHLLGGLDKPSEGSVLIGGHNLTDLDDDKLTELRRRKIGFIFQFFNLIPVLNA
                     TENAALPVTLDGLKPADAQKKAVEWLTRFGLADRLNNRPDQLSGGQQQRVAIARALVA
                     DPELILADEPTGNLDTRSSDEIASLLRDVSKKYNRTIVMVTHDPRIAAYADRIIFLKD
                     GKVVDETLLERKNGKSADFVAGKVKELGD"
BASE COUNT          380 a          380 c          394 g          368 t
ORIGIN      
        1 tagtaactcg tataatattt tcccgttttg taattagtaa ctcgtatact attttcttgt
       61 gaggctattt tgtctgttcg aaatgccatt ttaggattgc tcgcccaaag acctcggcat
      121 ggatatgagc tgcattccgc gttttccacg gtggtgggcg gcgcgacatg ggatgtgaaa
      181 cctgctcaga tctataccac gttggaacgc ctggaagaat ctgggttggt gcaaaccaaa
      241 tctgacctgg gcgaggggcg tgaaccggat cgacgcatct acgccatcac acgcaatggg
      301 catgaggcgt tgaagagttg gttcaatgaa ggcgtgccga ccgagcatca gcgtgacgag
      361 ttctttatca aattgatggt tggcttgatc tctggtgaag ccgacccggc acgcatcatt
      421 cagacccagc gttcgcggct ttttcaagaa atgcatgatg ccaccaccca gcgtgatacc
      481 tacgacccgc gcatagagat ggcgcagatt cttttagtgg acaaagccat tatgcacctc
      541 gaagcggact tgcgctggct ggacatgatc gagatgcgtt tggaaaccat caaagatcaa
      601 cccatccctg agccggaaac tcgcaaacgc ggcagaccca agaagatcga cgctggctcc
      661 acatatccgc cgccgagtga gaagtaagta tttgttgatc aggtttttgc ttgatgtttc
      721 ttaagcaagc tttattcgtt tgtatggttt tacagaaaga aataaaaaat gtccaatatt
      781 cagatcgaaa aacttaccaa aacctatggc tcaggcgtta ccgcagtagc agcgcttgac
      841 catgttaacc tcaccatccg cgagggcgaa tttgtcgcag tcatgggacc cagcggctgt
      901 ggaaaatcga cgctgttgca tttgctcggc ggcttggata aacccagtga aggcagtgtg
      961 ttgatcggcg ggcacaatct gaccgacctc gacgatgaca agctcaccga actgcgccgc
     1021 cgcaagatcg gtttcatctt ccaattcttc aacttgattc cggttttgaa tgcaaccgag
     1081 aacgcggctc tgcccgtcac gctggatgga ctcaaacctg ctgatgcgca aaagaaagcc
     1141 gtggagtggt tgactcgttt tggcttggca gatcgcttga acaaccgccc cgaccaactc
     1201 tctggcggac agcagcaacg cgtcgccatt gcccgtgccc tggtcgctga cccggagctg
     1261 atcctcgctg atgaacccac cggcaacttg gatacccgct ccagcgacga gatcgccagt
     1321 ttgttacgtg atgtctcgaa aaaatataac cgcaccattg tgatggtgac ccacgatcca
     1381 cgcattgccg cttatgctga ccgtatcatc ttcctcaaag atggcaaggt ggtggatgaa
     1441 actttgcttg aacgaaagaa tggaaaatct gcagattttg tagcaggcaa ggtgaaagaa
     1501 ctgggtgatt gaaatgaagt tt
//
LOCUS       DAOQNJ010000627         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_79846_length_1521_cov_5.329468, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000627 DAOQNJ010000000
VERSION     DAOQNJ010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79846_length_1521_cov_5.329468"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLG52_13650"
     CDS             <1..887
                     /locus_tag="PLG52_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB42546.1"
                     /translation="SGTLSAIIFATVLGWYITDTHLLFSILGVGLGLISIIPPLVVFR
                     ITKEKPSEEQGEPLSWRESMKLTLNNRPFRFVMGLYLLSWTTASILAAMLVYFANYRL
                     RVPEQANYFVLAAQFSAILFIPFWVWVAKKLDKRRAFILGTFTWIAVLLGLSALPADG
                     IALAYVLAALAGSGIATAYVLPWSMIPDIIEHDQLQTGQRREGSYYAFASFFQKLATA
                     LALWGMGQALAMNGYITPDASGSLPIQPDSAIQAIRLFTGPIPTVLLLLAVVFAWNYP
                     ITRESHNETLKVLAEREA"
     gene            889..1197
                     /locus_tag="PLG52_13655"
     CDS             889..1197
                     /locus_tag="PLG52_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42547.1"
                     /translation="MLPRHQTCSICSNLQDHQSATEYVISPEENIDFPKNVLNAIGKL
                     KKVKEISSSTDIFQCPECETYYMYSVSYEYLVSGSGSYDEYNLSRMNDETAKKFLLDD
                     "
     gene            1224..1511
                     /locus_tag="PLG52_13660"
     CDS             1224..1511
                     /locus_tag="PLG52_13660"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNB42548.1"
                     /translation="MILEFKGKIFQWRGPAPYLFLAVPDKESADIKAVSKLVTYGWGV
                     IPVMVTVGKTEWKTSLFPKDGRYLVPIKMLAQKAENLKEGDEIKLRLEMRL"
BASE COUNT          372 a          378 c          366 g          405 t
ORIGIN      
        1 tcagcggcac attgagcgcg atcatttttg caaccgtatt gggttggtac atcacagaca
       61 cgcatttgct tttcagtatc cttggcgttg ggttgggact catctccatc attccgccat
      121 tggtagtgtt tcgcatcacc aaagaaaaac cttccgaaga acaaggcgag ccgctttcat
      181 ggcgcgagtc gatgaagctg accttgaaca atcgaccatt tcgttttgtg atgggtcttt
      241 atcttttatc gtggacaact gccagtattc tcgccgcgat gttggtgtat tttgccaatt
      301 accgtttgcg tgtgccagag caagccaatt attttgtcct tgccgcgcag ttctccgcga
      361 tccttttcat tccattttgg gtgtgggtgg caaagaagct cgacaaacgc cgcgccttca
      421 ttcttggcac tttcacatgg atcgcagtac tgctcgggct ttcggcattg cctgccgatg
      481 gcattgctct ggcgtatgtg ttggcggcgc tggcaggctc tggcattgcc actgcctatg
      541 tgttgccgtg gtctatgatc cctgacatta tcgaacatga ccaactgcaa accggtcaac
      601 gccgcgaagg ttcgtattac gcattcgctt cattctttca gaaacttgcc acagcactcg
      661 cgctctgggg catgggtcaa gccctcgcaa tgaatggata catcaccccc gatgcgagcg
      721 gaagtttacc cattcaacct gattctgcga ttcaagccat ccgcctcttt accggaccga
      781 taccgactgt cctgcttctt ttggctgtag tcttcgcctg gaactatccc atcacccgcg
      841 aaagccataa tgaaacgctg aaagtgttgg cagaacgaga agcgtgacat gctcccccgc
      901 catcaaactt gcagcatttg ttccaatctt caagatcatc agtccgcaac ggagtatgtg
      961 atcagcccgg aagagaatat tgatttccct aaaaacgtac tgaacgccat cggaaaacta
     1021 aagaaggtaa aagagatttc ctccagtact gatatttttc agtgtcccga atgcgagacg
     1081 tattacatgt atagcgtgag ctatgaatat ctggtcagcg gtagcggttc gtatgatgag
     1141 tacaacctgt ctcgtatgaa cgatgaaacc gcgaagaagt ttctgttaga tgactgactg
     1201 caataggcgt tatacttcac actatgatcc ttgaattcaa aggcaagatc ttccaatggc
     1261 gcggaccggc tccctatctc ttcttggcag taccagataa agaaagtgca gatattaagg
     1321 cagtctccaa attagtgacc tatggttggg gcgttatccc tgtcatggtg acggttggca
     1381 agaccgaatg gaagacatcg ctttttccga aagatggacg ttaccttgtg ccgatcaaga
     1441 tgctcgcgca aaaagcggag aatctcaaag aaggcgacga gataaagctt aggctagaaa
     1501 tgcggttgta gacatcacaa c
//
LOCUS       DAOQNJ010000628         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_80343_length_1516_cov_10.266940, whole genome shotgun
            sequence.
ACCESSION   DAOQNJ010000628 DAOQNJ010000000
VERSION     DAOQNJ010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80343_length_1516_cov_10.266940"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..>1516)
                     /locus_tag="PLG52_13665"
     CDS             complement(86..>1516)
                     /locus_tag="PLG52_13665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB42549.1"
                     /translation="EMAEVSAERLAKLIKADGCYLTLWNDVTKHVIPVAAYGTGKDTY
                     ARTSAKESEKTFTQSSLELERTLIVEDVYNTPYASASVVSRFPAKSLLILPLIALEKK
                     LGAVLVSFETPHRFIEEEVQICEQAASLIALALEKFQAMDEAQRRANTSESLRRVGIA
                     VAEQTELKQAVNYVLEQLKQMVPYDSASVQLRNGDELYIIGGQGWGDEQEIIDLRFPI
                     PGDNPNTVVIERGSPYYLPDAGEVYKQFRKPPHNHIRSWLGIPLLVQEKIIGLLAIDS
                     AEPNDFSEQEIMIATEFANQVAVTLENARILKETQTQAVTDPLTGISNRRGLIEAGKL
                     EFSKTHTTNRPFSAIMLDLDYFKSINDTYGHAVGDLVLREFAKRCKSCIREIDYIGRY
                     GGEEFIILLPDAHMKASMNIAERLRSAITKKPIRIDDKEISISASLGVACRDENTTSL
                     ETLIARADQALYIAKHNGRNCVAVSH"
BASE COUNT          378 a          340 c          343 g          455 t
ORIGIN      
        1 cagagcaagc ccaaatgaag gtggaggcgt cttttctatc cgatggggaa aatggtaagt
       61 tggggttgta tcaaatttgg cgggcttaat gactgaccgc tacgcaattt cgcccattat
      121 gcttagcaat atataaagct tggtctgcac gagctatcaa cgtttccagc gatgttgtat
      181 tttcatctcg gcatgccacg ccaaggctgg cagagatact gatttctttg tcgtcaattc
      241 gaatcggctt tttcgtgatg gcacttcgca aacgttcggc gatgttcatg cttgctttca
      301 tgtgagcatc tggtagcaga atgatgaatt cttcgccgcc atatcgtcca atataatcta
      361 tttcgcggat gcaactttta caacgcttgg cgaattcgcg caaaacaaga tcgcccaccg
      421 cgtgcccata cgtgtcattg atagacttga aataatcaag atccaacatg atcgctgaaa
      481 acggacgatt tgtagtatgt gtttttgaaa attcgagctt gcctgcttca atcaacccgc
      541 ggcgattgct aatgcctgta agcgggtcgg ttactgcctg tgtttgggtt tctttcaata
      601 tcctagcgtt ctcaagtgtg actgcaactt gattggcaaa ttccgtggcg atcatgatct
      661 cttgttcgct aaaatcattg ggctcagcac tgtcgatggc gagcaatcca atgatctttt
      721 cctgtacaag taagggaatg ccaagccacg aacggatgtg gttatgcgga ggttttctga
      781 attgtttgta tacctcacct gcgtctggta agtaatatgg agaccctcgt tctataacaa
      841 ctgtgttggg gttatctcct gggatgggga agcgtaggtc aataatttct tgctcatcgc
      901 cccaaccctg acctccaata atatagagtt catctccatt acgaagttgt acagaagcac
      961 tatcgtatgg caccatttgt ttaagctgtt caagaacata gttgaccgcc tgtttaagct
     1021 cggtctgttc ggcaacagcg atgcccactc ttcgcagact ctctgatgta tttgcgcgcc
     1081 gctgggcttc atccatggct tgaaattttt caagagccaa ggcgatgaga gaagctgctt
     1141 gttcgcagat ttgtacttct tcctcaataa agcgatgagg agtttcaaat gaaactagaa
     1201 cggcccccaa ttttttttct aatgcaatta atggcaaaat caaaagcgat tttgctggaa
     1261 aacggctaac caccgaagcc gaggcatagg gcgtgttgta aacatcttca acaataagtg
     1321 ttctttctaa ttcaaggctc gattgtgtaa aggtcttttc actttctttt gcagatgtac
     1381 gagcatatgt gtctttgcct gtcccataag cagcgaccgg aataacatgc ttcgttacat
     1441 cattccataa ggtgaggtaa cacccatctg ctttaattaa ctttgctagg cgttctgctg
     1501 atacttcagc catctc
//
LOCUS       DAOQNJ010000629         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0499
            NODE_81228_length_1507_cov_6.381543, whole genome shotgun sequence.
ACCESSION   DAOQNJ010000629 DAOQNJ010000000
VERSION     DAOQNJ010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810131
            Sequence Read Archive: SRR3501855
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:25:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,706
            CDSs (with protein)               :: 2,706
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81228_length_1507_cov_6.381543"
                     /isolate="AMR_MDS_0499"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PLG52_13670"
     CDS             <1..716
                     /locus_tag="PLG52_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNB42550.1"
                     /translation="GRSVMVGNKRMFEARGISLGSLESEVTRLQSEAKTAMLMAVDNV
                     AAGVIAVADTIKDSSKDAIADLHKMGLKVAMITGDNQKTAEAIAKQVGVDTVLAEVLP
                     EGKSAEVKKLQQEIVNRQSKILNVVAMVGDGVNDAPALAQADVGLAIGTGTDVAMAAA
                     PVTLMSGDLRGVSRAISLSRKTVTTIKQNLFWAFIYNVILIPAAILGFMNPMLAAGAM
                     AFSSVFVVTNSLRLRGVKI"
     gene            765..989
                     /locus_tag="PLG52_13675"
     CDS             765..989
                     /locus_tag="PLG52_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42551.1"
                     /translation="MDSESPVTDHQLPLTKSACGGILKDPSAYPSAMFNGERVYFCNQ
                     ACLKAFLSAPEAFMAGEVEHPLDDDETPES"
     gene            1019..>1507
                     /locus_tag="PLG52_13680"
     CDS             1019..>1507
                     /locus_tag="PLG52_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB42552.1"
                     /translation="MKKIVVLLLAVVLLTLTACAPKAAEMPKAELPAGKAWAEGKEIY
                     FIHTEASDEGVAQALTNMMKSPVILVPSLANVPDESLANVYVFTNGIAGSGPFGFQAD
                     VFDNPPGSDGYTPLRRLNAVTWTDGTKARELTSAADVLAAETVGELTIEQPGVVINMP
                     FVV"
BASE COUNT          391 a          362 c          396 g          358 t
ORIGIN      
        1 aaggaagaag cgtaatggtt gggaacaaga gaatgtttga ggcgagagga atctcgttgg
       61 gcagtcttga atccgaagtg acgcgtcttc aatctgaagc gaagaccgcc atgttgatgg
      121 cggtagataa tgttgcggca ggagtcatcg ccgtagcaga taccatcaag gacagctcaa
      181 aagatgcgat cgctgacttg cataagatgg gcttaaaagt tgcgatgatc actggcgaca
      241 accaaaagac agccgaagcg attgccaagc aagttggcgt cgatacagtt cttgccgaag
      301 ttcttcctga aggcaaatca gctgaggtca agaaactcca acaagaaatc gtcaatcgtc
      361 aatcgaaaat cttaaatgtt gtcgccatgg tcggcgacgg cgtgaatgat gctcccgcgc
      421 ttgctcaagc ggatgtcggt ctggcaattg gaacgggaac cgatgttgcc atggctgccg
      481 cccctgtgac cttgatgagc ggcgacctgc gcggtgtgtc gcgtgcgatc agtttgtctc
      541 gtaagacggt cacgaccatc aaacaaaacc tattctgggc attcatttac aacgtgattc
      601 tcatccctgc cgccattctc ggatttatga acccgatgct cgccgctggt gcgatggcgt
      661 tcagcagtgt ttttgtggtg acgaatagct tgcgattgcg tggtgtgaag atctagtaaa
      721 tggtgaatgg taaatagtga atggtgaata gtgagcatta ggctatggac agcgaatctc
      781 cagttaccga tcaccaatta cctcttacta aatccgcctg cggtggaatc ctgaaagacc
      841 catccgctta cccaagcgcg atgttcaatg gagagagagt ctacttttgt aatcaagcct
      901 gcttgaaagc ctttctgagt gcaccagaag cgtttatggc aggtgaggtg gagcatccct
      961 tagacgatga tgaaacaccc gaatcataaa tagacccatt gaaaaggaaa cccttaacat
     1021 gaaaaaaata gttgttcttc ttctcgcagt cgtactcctt acgttgactg cctgtgcccc
     1081 gaaagccgct gaaatgccaa aagccgaatt gcccgctggc aaagcctggg cagaaggcaa
     1141 ggaaatttat ttcatccata ctgaagcgtc agacgaaggt gtagcccaag cgctcaccaa
     1201 catgatgaag tcacctgtga tccttgtgcc atcgctggca aatgtgccgg atgaatcact
     1261 tgcaaatgtc tatgttttca ccaatggcat tgctggttca ggtccgttcg ggtttcaagc
     1321 tgacgtgttc gataaccctc ccggcagtga tggatacacc ccgctgcgcc gcctcaatgc
     1381 ggtgacatgg acagacggaa ccaaagcccg cgaattgact tccgctgctg atgtgctggc
     1441 tgccgaaact gtcggtgaat tgaccatcga acagcccggt gtcgttatca acatgccctt
     1501 cgtcgtt
//
